LOCUS       DAORPD010000001         7233 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_1898_length_7233_cov_2.434940, whole genome shotgun sequence.
ACCESSION   DAORPD010000001 DAORPD010000000
VERSION     DAORPD010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7233
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1898_length_7233_cov_2.434940"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(39..1001)
                     /locus_tag="PK976_00005"
     CDS             complement(39..1001)
                     /locus_tag="PK976_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRC66302.1"
                     /translation="MKKILVIGSTGQIGSELTLALRKEFGGENVIAGIRKTAPSKQIL
                     ETGPCEVVDATIREQIERAVEKHDIDTIVHMAAILSAVGEQNPMMAWNVNMNGLINVL
                     ECARDRKMDRVLVPSSIAVFGPGTPLDNTPQETILRPTTMYGITKVAGELLGDYYVLK
                     YGLDVRGLRYPGIISHETLPGGGTTDYAVAIYYEAVKNKKYTCFVREDTRLPMMYMPD
                     CLKATISLLKADFNKLRHHCDFNVSAMSFSVKELADSIKKYIPEFEVSYEPDFRQKIA
                     DSWPNSIDDSYAREEWGWKPDYDLDAMTRDMLSALQRKHQQGFI"
     gene            1220..2410
                     /gene="kbl"
                     /locus_tag="PK976_00010"
     CDS             1220..2410
                     /gene="kbl"
                     /locus_tag="PK976_00010"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HRC66303.1"
                     /translation="MYKEFQEFLSKELSAIEEAGLAKNERIIVSPQGAEITLNTGQKV
                     LNFCANNYLGLSNNPKLIEAAKAAMDTHGFGMSSVRFICGTTDLHKKLEAKIAEYFGT
                     EDTILYAACFDANGGVFEPLLSEEDTIISDALNHASIIDGVRLCKAVRYRYANADMKE
                     LEERLKDSQKQRFRLIVTDGVFSMDGNVAPIDKIYELANKYDAMVMIDESHSAGVVGQ
                     TGRGVTELFKLEGKIEIITGTLGKAFGGAIGGFTTGKKEIISMLRQRSRPYLFSNSLP
                     PMVVAAGIRMFDMMKETNDLQDKLHKNTAYFSQRMSEIGFDIKPTQSAIIAVMLYDAK
                     LAQDFAARLLDEGIYVTGFSYPVVPKGQARVRVQISAAHEKEHLDKAIAAFTKVGKEM
                     GIIK"
     gene            2565..3473
                     /locus_tag="PK976_00015"
     CDS             2565..3473
                     /locus_tag="PK976_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66304.1"
                     /translation="MENTNDQKSNKGIIIAGVVILLLAIFFGWKWVSTQSEISQIKKI
                     AEEEKAILRHELDSVMAEHERIKAAYGELADSLRVKDSIIQASALEIKKLLDTKWEYI
                     KVSRKLDQLRLVAQSYLRQMDSLYTVNRQLQEENLQMKEEIIAEQQKAKQLVQEKELL
                     TTKIKQGSALSISSLTAETLRLRSGGKEEFTDKGKRIDKIRVCFIIAENRIAQPGTRT
                     FYVRIADPNGNILTRSRGDEYSFTYQGEQLQYSTMANIDYQNKAVKMCADYNKLPLQE
                     KFISGTYQVAVYLGDDLLGETSFTVK"
     gene            complement(3677..4291)
                     /gene="udk"
                     /locus_tag="PK976_00020"
     CDS             complement(3677..4291)
                     /gene="udk"
                     /locus_tag="PK976_00020"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005802905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HRC66305.1"
                     /translation="MLTIGIAGGSGSGKTTVVKKITQLLPPDSVSVLSQDAYYYDNSH
                     LPPDERVKINFDHPSSIEFPLLNRHIDLLMEGERIPMPIYSYLTCSRAKDTITIYPRK
                     VIIVEGILILTDPDLRSRLVVKMFVDASPDERLMRIIQRDLVERGRSLQQTLEHYQNF
                     VKPMHEQFIEPTKRFADIIVPQGGENHVAIRLLAAQIKERLGLP"
     gene            complement(4389..5552)
                     /locus_tag="PK976_00025"
     CDS             complement(4389..5552)
                     /locus_tag="PK976_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004289786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HRC66306.1"
                     /translation="MAENEGFLKFIVDHLEQFFSYTAFANITVGHIIMICVGLFFIFL
                     AITKEYEPLLLVPIGFGILIGNIPFYPGLKVGIYEDGSVLNYLYFGVVQGIYPPLIFL
                     GIGAMTDFSALLSNPKLILIGAAAQFGIFGAYIAALALGFAPNEAGAIGIIGGADGPT
                     AIFLTSKLAPELLGAIAISAYSYMALVPVIQPPIMRLLTTPKERVIRMKPPRVVSKNE
                     KILFPIIGLLLTCFIVPSGLPLLGMLFFGNLLKESGVTKRLADTAKGPLIDIVTILIG
                     LTVGASTQATTFLTPKCIGIFALGAFSFMVATAAGVLFVKFLNLFLKPEDKINPLIGN
                     SGVSAVPDSARLSQVIGLEYDKTNYLLMHAMGPNVAGVIGSAVAAGILLSFLY"
     gene            complement(5554..5997)
                     /locus_tag="PK976_00030"
     CDS             complement(5554..5997)
                     /locus_tag="PK976_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HRC66307.1"
                     /translation="MKRFKFKINGNTYETEIIDIEDNIATIEINGSRYTVEVDQTLTP
                     VKTPKLMRTPAVPSTDIHPAVSKTSAPIGPKGTGYIKSPLPGVILDILVKEGDYVKIG
                     QKVIVLEAMKMENNIESDKEGRVVSIKKQKGDAVLEGDILIVIGE"
     gene            complement(6039..6920)
                     /locus_tag="PK976_00035"
     CDS             complement(6039..6920)
                     /locus_tag="PK976_00035"
                     /inference="COORDINATES: protein motif:HMM:NF016187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family transporter subunit"
                     /protein_id="HRC66308.1"
                     /translation="MKHFRFFIIIASILPLIGFSQGVNSLKINEILVINDSNYIDDFG
                     ERNGWIEIFNANYNYVDMGGMYLTNDLSNPTKYYIPKGDPRTVIPPRGFIVFYANNKP
                     TRGIMHLNFELKGSKVVAIFSSDGKTLIDKLDIPEEQHPDVTYGRLRDGGPELGYLEK
                     TTPKASNVTEQVATPAEIFGEMDPHGIAITIIAMSVVFVSLALLYILFKNTSKIYSLD
                     LSSIFKRKKGPQKEEEKAKIGESIPGEVAAAIAMALYQYHAQIHDVEKTVLTIRRVSR
                     TYSPWSSKIYGLRKSPR"
     gene            complement(6944..>7233)
                     /locus_tag="PK976_00040"
     CDS             complement(6944..>7233)
                     /locus_tag="PK976_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009128927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRC66309.1"
                     /translation="PTAEIAVMGPKGAIEILYNKKIQEIADPAEREDFILNAEKEYKD
                     KFANPYNAARYGYIDDIIEPRNTRFRVIRAFQALATKKDTNPPKKHSNIPL"
BASE COUNT         2266 a         1455 c         1447 g         2065 t
ORIGIN      
        1 tgcaatgcag ggcttcaatc ttattgtaaa tcaatttttt aaataaatcc ttgctggtgc
       61 tttctttgta atgcactcag catgtcgcgt gtcatggcat cgaggtcata atcaggtttc
      121 cagccccatt cttcacgagc atacgaatcg tctatgctat tgggccagct gtcggcaatt
      181 ttttgacgga aatcgggctc ataagaaacc tcgaattctg gaatatactt tttaatgcta
      241 tcggccaatt ccttaaccga aaaactcata gccgaaacgt tgaagtcgca atgatgccta
      301 agtttattga aatcggcttt taataatgat atagtagcct taaggcaatc gggcatatac
      361 atcatcggta atcgggtatc ttcacgaaca aaacaagtat attttttatt cttaacagct
      421 tcataataaa tagcaactgc ataatcagta gttccaccgc ctggaagtgt ctcatggctg
      481 atgattccgg gataccgcaa tccacgcaca tcgagtccgt atttcaacac ataatagtcg
      541 cccaataact caccggcaac tttagtaatg ccatacatgg tggttggtcg aagaatggtt
      601 tcctggggag tattatcgag aggggtaccg ggtccgaaaa cagcaatact gcttggaacc
      661 aaaactctat ccattttacg atcgcgggca cattccaata cattgataag cccattcata
      721 ttgacattcc aggccatcat gggattttgt tctcccacgg ccgaaagtat agctgccatg
      781 tggacaattg tgtcaatatc gtgcttctcg acagcccgtt ctatttgctc acggatagta
      841 gcatcaacga cttcacaggg ccctgtttcc aaaatttgct tagaaggtgc agttttacga
      901 atgcctgcaa ttacattttc cccaccaaat tctttccgta aggcaagggt gagttctgac
      961 cctatctgtc ccgtagagcc aattactaaa atttttttca tatcgcaatt tttgaattta
     1021 ttataaatgt taatctatta acgatagctc ggcaaaagta cgggattttc gtcatctcta
     1081 aggcagattt aaagatgatt cgagatgaaa tcgcccgtta taaatttccc gaaagaacgg
     1141 aggattaaac catctgttac aaataatcta taattttgca actttaaaaa atcatcttat
     1201 catttaattt aatacagcta tgtataaaga atttcaggaa tttctcagta aagaattatc
     1261 agcaattgaa gaggccggct tagcaaagaa tgagcgtatc attgtgagtc cgcaaggggc
     1321 agaaataacg ctgaacactg gccaaaaagt gttgaatttt tgtgctaata attacttggg
     1381 gctttccaat aatcccaaat taatagaagc agcgaaggct gcgatggata cgcatggctt
     1441 tggcatgtca tcggtaagat ttatttgtgg tactaccgac ttgcataaga aactcgaggc
     1501 taagattgcc gaatattttg gtaccgaaga taccattctt tacgctgcct gtttcgatgc
     1561 aaatggtgga gttttcgagc cattgcttag cgaggaagat acaattatta gtgatgcttt
     1621 gaatcatgct tccatcattg acggagtaag actttgtaag gcagtcagat acagatatgc
     1681 caatgccgat atgaaggaat tggaagaacg acttaaggat tctcagaaac aacgttttcg
     1741 cctcatcgta accgatggcg tattcagtat ggatgggaac gttgctccta tcgacaagat
     1801 ttatgaactt gctaataaat atgatgctat ggttatgata gacgaatcgc attctgcggg
     1861 agtagttggc caaacaggaa gaggagtcac tgagcttttt aaattggagg ggaaaatcga
     1921 gatcatcaca ggcactttag gaaaagcctt tggtggagcc atcggtggtt tcaccacagg
     1981 gaaaaaagaa attataagta tgttgcgaca gcgttccaga ccttacttgt tcagcaattc
     2041 tttacctcct atggttgttg ctgcaggcat ccgcatgttc gatatgatga aagaaacaaa
     2101 cgatttacag gataaactgc ataaaaatac agcttacttt agccagcgta tgtcggaaat
     2161 tggattcgat ataaagccta cacaaagtgc catcatcgcc gtgatgcttt acgatgcgaa
     2221 attggcacaa gattttgcgg cgagactttt ggacgaagga atttatgtaa caggattctc
     2281 ctacccagtg gttcctaaag gtcaagcaag agtaagggtt caaatttcgg cagctcatga
     2341 aaaggaacat ctcgataaag caatcgccgc cttcactaaa gtagggaaag aaatgggaat
     2401 catcaaataa gcgattctta ctttttagcc gatattttca aatttttcac agccccggcc
     2461 aaataccggg gttgtttatt agtatgccaa tatttctatt atatttgcat taaaatcgaa
     2521 agaattgaga atatagttgc caaaaccaat aaaaacttag agaaatggaa aacacaaatg
     2581 atcaaaaatc gaacaaaggc ataatcattg ccggtgtagt cattttgctc ttggcaatat
     2641 tttttggatg gaaatgggtg agtacccaat cagagataag tcaaatcaag aagattgccg
     2701 aagaagaaaa agccatcctc cgacatgagc tcgattctgt aatggccgag cacgaaagaa
     2761 taaaggctgc atatggcgag cttgccgaca gcctgagggt caaagacagc atcattcagg
     2821 caagtgcgct cgaaataaag aaactcctcg atacgaaatg ggaatacatc aaagtttccc
     2881 gtaaactcga ccaactcagg ttagtagctc aatcctatct caggcaaatg gatagcctgt
     2941 ataccgtaaa ccgacagcta caggaagaaa acctacaaat gaaagaagaa atcatagccg
     3001 aacagcaaaa ggccaagcaa ttggttcagg aaaaagaatt gcttacaacc aagatcaaac
     3061 aaggctcggc attgagcatc tcgagcctca ctgccgaaac attacgattg cgaagcggag
     3121 gtaaggaaga atttacagac aaaggaaaac gaatcgacaa aataagagtc tgctttatca
     3181 ttgccgaaaa ccgcattgca caacccggca cacgtacttt ctatgtacga attgccgatc
     3241 ccaatggaaa tattcttacc cgaagcagag gcgatgaata ttcatttact taccaaggtg
     3301 aacaattaca atattctaca atggccaata tcgattacca gaacaaggct gtgaaaatgt
     3361 gcgctgatta caacaaactg cctctacaag aaaaatttat ctcggggacc tatcaggtgg
     3421 ctgtttatct tggagatgat ctccttggag agaccagctt cactgtaaaa taaacttacc
     3481 ctctgttttt gattttactc acctataggg cttttcgcgt attcttacct tattcaactc
     3541 gatgcaagac ctttagatag cgcaagttta taaataatat ccataaggaa ttgcagcggg
     3601 gttcattgtt tatgatccgc agatattcat atataattat tcaacatcat gaagtcgtgc
     3661 aaaagaatgc atcgaattat ggcaatccca gtctttcctt aatttgggcg gcaagcaatc
     3721 ttatggctac atgattttca cctccctgag gaacgataat atcggcaaag cgtttggtag
     3781 gttcgatgaa ttgttcatgc atgggtttta cgaaattctg ataatgctcg agggtttgtt
     3841 gcagcgagcg cccacgttct accaaatctc gctgaataat acgcatcagc cgttcatcgg
     3901 gggaagcgtc cacaaacatc ttcacaacga gcctgcttct taggtcagga tctgtaagaa
     3961 taagaatgcc ttctacaata atgaccttac gaggatatat agtgatagta tcttttgccc
     4021 tgctacaggt aagataactg tatataggca tggggattcg ttcaccctcc atgagtaaat
     4081 cgatatgccg attcaacaga gggaattcga tggaagaagg gtgatcgaaa ttaatcttta
     4141 ccctttcatc gggaggcaga tggctattat cgtagtaata agcatcttgc gacaatacag
     4201 ataccgagtc tgggggaagt agttgggtaa ttttcttgac aactgttgtt tttcctgaac
     4261 ctgaaccacc tgcaattcct atcgtcaaca tagaatcctt tataattgca tttcaaaata
     4321 aattctaaac gcgcaacact ttataatttt ctaaaaatta ccctctgtca tttccaatca
     4381 ttccataatt aatacaagaa gctaagtaaa ataccggcag caacagcact tccaattact
     4441 ccggcaacat ttggtcccat agcgtgcata agcaaataat tggttttatc gtattcgagg
     4501 ccaataactt gagaaagcct ggcactatcg ggcacagcag atactcctga attcccgatc
     4561 aaaggattga ttttgtcttc tggtttcaag aaaagattga gaaatttcac aaataatact
     4621 ccggctgcag tggctaccat aaaagaaaaa gcaccaagag caaaaatacc aatgcacttt
     4681 ggggtaagaa aagtagtagc ctgagtagaa gctcctacag ttagtcctat aagtatagta
     4741 acgatatcga ttaaagggcc ttttgcggta tcggccaggc gtttggtaac tccactttcc
     4801 ttaagtaaat tgccaaaaaa cagcattccc aagagtggaa gacctgaggg gacaataaag
     4861 caggtaagca atagcccaat gatagggaat agaatttttt catttttcga taccactctg
     4921 ggaggtttca ttcggattac acgctctttg ggggttgtaa gcaatcgcat gataggtggt
     4981 tgtatgacgg gtacaagagc catataagaa taggccgaaa tggcgatagc tcccaacaat
     5041 tcaggagcta attttgaagt taagaatatt gcggttggcc cgtcggcacc accaataata
     5101 ccaatagcac ctgcttcgtt gggagcaaat cctaaagcaa gcgcagcaat ataagcacca
     5161 aatataccaa actgcgctgc agctcctatg agaataagct tcgggttcga aagaagagcc
     5221 gaaaaatcgg tcattgctcc tatgcctaaa aagatcagag gaggatatat cccttgaacc
     5281 acaccaaaat ataaataatt caaaacgctt ccatcttcat aaattccaac ttttaatcct
     5341 ggataaaaag gtatattgcc aattaaaatt ccaaatccaa tgggaaccag caacaagggt
     5401 tcatattcct tggtaatagc caaaaaaatg aaaaacaatc ccacacatat catgatgata
     5461 tggccaacag tgatattagc aaaagcagtg taactaaaaa actgctctaa gtgatccact
     5521 ataaatttca ggaatccttc gttttcagcc attttattct ccaattacga ttaatatatc
     5581 tccttccaat actgcatctc ccttttgctt tttaatgcta accacacgcc cttccttatc
     5641 gctttcgata ttgttttcca tcttcatggc ctcgagaacg attacctttt ggccaatttt
     5701 gacataatca ccctctttga ccaaaatatc gagaattaca ccaggcaaag gcgatttaat
     5761 ataacccgtc cctttagggc ctataggtgc actcgtctta gaaacagcgg gatgaatatc
     5821 cgtagaggga actgcaggtg tacgcatgag tttcggtgtc ttcacgggag taagggtttg
     5881 atcgacttcg actgtatagc gagaaccatt gatttcgata gttgcaatat tatcctcgat
     5941 atcaattatc tcggtttcgt aagtgttgcc atttatttta aacttgaatc ttttcatctt
     6001 atttaaactg actttgaata atagaactaa ataaatattt aacgaggtga ttttctgagt
     6061 ccataaattt ttgaactcca tggtgaatag gtacgggaga ctcttcgaat cgtaagaaca
     6121 gtcttttcta cgtcatgaat ctgggcatga tattgatata gagccatagc aatagccgca
     6181 gcaacttcac ctggaataga ttctccaatc ttcgcttttt cttcttcctt ttgaggccct
     6241 ttttttcgtt taaatatcga acttaagtcg agagaataaa tcttggaagt gttcttgaac
     6301 aagatatata gcagggcaag agaaacaaat actaccgaca ttgcaatgat agtgattgca
     6361 atgccatgag gatccatttc cccaaaaatt tcagcaggtg ttgccacttg ctcagttaca
     6421 ttggatgcct taggggttgt cttttcgaga taaccaagct cagggccacc atctctcaag
     6481 cgcccataag tcacatcggg gtgttgctcc tcggggatgt ctaatttgtc gatgagagtt
     6541 tttccatcgg aactgaaaat agcaacgact tttgaaccct tcaattcaaa attcaaatgc
     6601 attattccac gagtaggctt attattggca taaaagacga tgaatccacg gggagggatt
     6661 actgtacggg gatcaccttt aggaatataa tatttggtag ggttggataa atcattcgtc
     6721 agatacatac cacccatatc cacataatta tagttagcat taaagatttc gatccagccg
     6781 tttctttccc caaagtcgtc gatatagtta gaatcgttga ttaccagtat ttcattgatc
     6841 tttaaactat ttacaccctg ggaaaagcca atcaagggta aaatgctggc aataataata
     6901 aaaaaccgaa aatgtttcat ggtttcttat aaatattatt tatttagaga ggaatatttg
     6961 aatgcttttt cggaggatta gtatcctttt ttgtagccag tgcttgaaaa gctctaatca
     7021 ctctgaaacg agtattgcgg ggttcaatga tatcgtctat atatccatat cgagctgcat
     7081 tatatggatt tgcaaattta tccttatatt ctttttcggc attcagaata aagtcctctc
     7141 tttcggcagg atcggcaatt tcttgtattt ttttattata aagaatttct atggcgcctt
     7201 taggacccat aacagcaatc tcggctgtag gcc
//
LOCUS       DAORPD010000002         6544 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_2154_length_6544_cov_2.262136, whole genome shotgun sequence.
ACCESSION   DAORPD010000002 DAORPD010000000
VERSION     DAORPD010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6544
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2154_length_6544_cov_2.262136"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="PK976_00045"
     CDS             <1..207
                     /locus_tag="PK976_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66310.1"
                     /translation="WHNVLPDKNNLFKPLLVNKMEDMTGKTNRILVEILHKNERLCFV
                     QSFTGQSSIETPPNRFSWKVISNI"
     gene            238..765
                     /locus_tag="PK976_00050"
     CDS             238..765
                     /locus_tag="PK976_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRC66311.1"
                     /translation="MKNIVLIALFSLLFLNMEGQDPKQSSKGRSLPSVELKTLDRKTL
                     LTDTLSNHGKPMIISFWALWCKPCIKELTNIGNVYKEWVEETGVRLIAVSIDDARSSS
                     RVKPFVDGKGWEFEVLLDPNSDFKRAMNVNLIPHTFLLDGNGKVVWQHTSYTEGSELE
                     LIEMVRKLNQGETIQ"
     gene            852..2576
                     /locus_tag="PK976_00055"
     CDS             852..2576
                     /locus_tag="PK976_00055"
                     /inference="COORDINATES: protein motif:HMM:NF039629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6029 family protein"
                     /protein_id="HRC66312.1"
                     /translation="MKKLLRITLPFVLLSCSLYVKSQDFIHNAQVSGTFQADANAYVN
                     DKLLHINDSTLNGKRLGINGFALIHYDYKNFSAGLRYEAFLPPLLGFSPDAEGSGIAN
                     WFVNYKVDNFEVTAGNFYEQFGSGMILRSYEEWTLGYDNALDGFRVKFKPYHGIYLKG
                     LMGYQRNYWVKYSEGNRGLVRGIDGELNFNELIEKLGNSPFRLSLEASFVSKYEKMPL
                     KTIIRSILPEGSYEVYQYKLPENVGVWLAAINLGYSNWNLMTEYVRKSENPSALNNYI
                     YREGQALLSVLTYSKKGLGIELSAKRIDNMGFKSRMEEKENVLDINFLPPLNPLHLYS
                     FATMYPYATQPNGEMAVHTGIEYTFPKNTLLGGKYGTNLSLSYSLIKDIEKQPVSADI
                     PIDSTGTLGYTSDFFAFGDRKFYEDLKIQLTKKFSQRLKMIAAYFNQVYDKDVIEGHT
                     NEYGKVYANIGVVDMTYRINNQHSIRLELQTLFTEQDKGNWMGTTIEYTLAPKWFFSL
                     MDEYNYGNDNPDDRIHYYNISFGYTHQSTRISLSYGRQREGLLCVGGICRYVPAYTGF
                     GLTLSSNF"
     gene            2669..3541
                     /locus_tag="PK976_00060"
     CDS             2669..3541
                     /locus_tag="PK976_00060"
                     /inference="COORDINATES: protein motif:HMM:NF033782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28 family outer membrane lipoprotein"
                     /protein_id="HRC66313.1"
                     /translation="MKKHHPTSVIIITWIFTMMQIACDKVDAPYLEHIQTYLDTAQTS
                     DTSYKRIALLEDFTGHKCVNCPQAAQLAKNLQLAHKGQIVVVEIHAGFFAVPDQSGLY
                     IADYRCTEGTSLNDYFNIMQYPTGLVNRKAYEGKIRLLPTSWESAILAELKQEPLAFI
                     QLENTFNPQNRNLRIETDIKILQNLEGEYTYTLYLIENHLISSQKNNNSFVGPVPDIT
                     DYVHNHVLRTSINGIWGETLHPGNGFVKDETIQLIHNFIMSENWNASQCHVVALMSKV
                     GTNEVIQAAEESIE"
     gene            3584..5692
                     /locus_tag="PK976_00065"
     CDS             3584..5692
                     /locus_tag="PK976_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HRC66314.1"
                     /translation="MNRKFLLSLLGIFFFLTQLPQDYAQVLDPVKWSFSSQKINSDEY
                     ELIFTAKIDKNWHLYSQYVPEGGPVPTNFIFEPSSNFKLIGKTTEPRPHKMYDQNFEM
                     EVQYFDGQAIFKQRIKSLTQQSFNVRGTVEFMACDDSRCIPPAEVDFDFAISPSAEPD
                     KAVTGVQKTETAYQLNTDSSFGEISEENIATSANDSVALNTSLGGEQKERHSNLWAFF
                     CLAFLGGLIAIFTPCVFPMIPMTVSFFMHDEENKKQARRQALIYGLSIIVIYTVIGTL
                     VALTLGPNFANFLSTHWLPNLFFFVIFMVFAASFFGAFDLTLPHWMANKADQNAEKGG
                     ILGPFFMAFTLVLVSFSCTAPIVGTILVASASGEILKPIIGMAGFGLAFAFPFTLFAL
                     FPSWLGTLPKSGGWLNSVKVVLGFIEVAFGLKFLSVADQTYHWGILDREIYIAIWIVV
                     FTLMGFYLLGKIRFKHDDEVHHIGVFRLTLVIATFSFVVYLLPGMWGAPLKALSGYLP
                     PQSSHDFDLNQIIRENVSANLNILPQQSNPSVPTEQCENPKYADIFHLPHGLKGYFDY
                     QQGLACAKQLNKPIFIDFTGHGCVNCREMEARVWSDPKVLQRLRNDFVIIALYVDDKT
                     PIPENEWVISSYDGKVKKTIGKKYADFQISKFNVNAQPYYVILDTNGQPLIQPKAYDL
                     NIDNFIQFLDAGKQAFYRKD"
     gene            complement(5862..5934)
                     /locus_tag="PK976_00070"
     tRNA            complement(5862..5934)
                     /locus_tag="PK976_00070"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5899..5901),aa:Lys,seq:ttt)
     gene            complement(6047..>6544)
                     /locus_tag="PK976_00075"
     CDS             complement(6047..>6544)
                     /locus_tag="PK976_00075"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HRC66315.1"
                     /translation="ANIYSPKALERMARIKGIKVEKAEFSIICHDLSQISEYCLPIPN
                     HIFKVMKRLLPGPYTFILKANSNVPRIFQSRKKTIGIRVPDNEIARAIVRELGNPIAN
                     TSIRDEDEILEYTTEPELIYEKYKTLVDIVVDGGYGDNVPSTVLDCTSNEIIIVREGK
                     GPIDF"
BASE COUNT         1960 a         1369 c         1281 g         1934 t
ORIGIN      
        1 tggcacaatg tgcttcctga caaaaacaac ttgttcaaac cacttttagt aaataagatg
       61 gaagacatga ccggcaaaac gaacaggatt ttggtcgaaa ttttgcataa gaacgaaaga
      121 ttatgctttg ttcaatcgtt taccggtcaa tcttccatcg aaactccacc caacagattc
      181 tcatggaaag taatttcgaa catctgatta attatactca ttcaatactt taacgccatg
      241 aaaaacatcg ttctgattgc attattttcc ttattgtttt taaatatgga gggacaggac
      301 ccaaagcaat cttcgaaagg tcgttccttg ccatcggttg aattgaaaac cttagacaga
      361 aaaaccctac ttacggatac tttgagcaac catggcaaac ccatgatcat cagtttttgg
      421 gcgctttggt gtaaaccttg catcaaggag cttaccaaca ttggcaacgt ttataaagag
      481 tgggtcgaag aaaccggagt aagactcatc gccgtttcta tcgatgatgc gcgttcttcg
      541 tcccgcgtaa aaccttttgt cgatgggaaa ggctgggaat tcgaagtttt gctcgatccc
      601 aacagcgact tcaagcgcgc catgaatgta aacctgattc ctcatacctt tctgctcgat
      661 ggcaacggca aagtagtctg gcagcatacc tcctacactg aaggaagcga actcgaactc
      721 atcgaaatgg tcagaaaact caatcagggc gaaaccatcc aataaattag aataatccaa
      781 tattgaccag aatgttcgaa aaatcgagga atcatctaag taataaaaat ttatttcttt
      841 ttattatcga tatgaaaaaa ttactgagaa tcactttgcc cttcgttcta ttgtcatgtt
      901 ctctatatgt aaagagtcag gattttattc acaatgcaca ggtaagtgga acttttcagg
      961 ccgatgccaa tgcctatgtc aatgataaat tactccacat caacgacagt acgttaaatg
     1021 gcaagagatt gggaataaat ggttttgctc ttattcatta tgattacaag aatttttcgg
     1081 caggcttgcg gtatgaagcc tttttgcctc ccttgctcgg attttcgccc gatgccgaag
     1141 gaagtggaat agccaactgg tttgtcaact acaaggtcga taattttgaa gtaactgctg
     1201 gaaatttcta tgaacagttc ggttcgggta tgatccttcg ttcgtatgaa gaatggacac
     1261 ttggctacga taatgcactc gatggattca gagtaaaatt caaaccttat catggaattt
     1321 atctgaaagg cttgatgggc tatcagcgca attactgggt aaaatattcc gaaggaaatc
     1381 gagggttggt aagaggcatc gatggggagt tgaatttcaa cgaactgatc gaaaaactgg
     1441 gaaatagtcc ttttcgattg agtttggaag caagcttcgt aagtaaatac gaaaaaatgc
     1501 ccctaaaaac cataatacgg tccattcttc ccgaaggcag ctatgaggta tatcaataca
     1561 aattgcccga aaatgtggga gtttggttgg cagccattaa tcttggctat tctaactgga
     1621 atctgatgac ggaatatgtt cgcaaaagcg aaaatccatc cgccttgaat aattatatct
     1681 atcgcgaagg ccaggctcta ttgagcgtac ttacttactc gaaaaaaggc ctgggaattg
     1741 aactctcggc caaacgaatc gataacatgg gctttaaatc gagaatggaa gaaaaggaga
     1801 atgttttgga tatcaacttc ctgccacctc ttaatccctt gcacctttat agcttcgcta
     1861 caatgtatcc ttatgcaact caacccaatg gcgaaatggc cgttcacacc ggaatcgaat
     1921 atactttccc caagaatacc ttacttggag gcaaatatgg cactaatctc agcctttcct
     1981 attcattgat caaagatatc gaaaaacaac ctgtttcagc tgatattccc atcgacagca
     2041 ccggcaccct gggctatacc tctgattttt tcgccttcgg agaccgcaaa ttctacgaag
     2101 acttgaagat acagcttaca aaaaagtttt cacagcggtt aaaaatgatt gcagcctatt
     2161 tcaatcaagt ctacgacaaa gatgtaatcg aaggacacac gaacgaatat ggaaaagtat
     2221 atgcaaacat tggagtcgtg gatatgactt atcgtatcaa caatcagcat tccatacggc
     2281 ttgaattgca aactctattt actgagcagg ataagggaaa ttggatggga accacgattg
     2341 aatacacact cgctccaaaa tggttctttt ccctcatgga tgaatataac tatggaaacg
     2401 ataatcccga tgaccggata cattattata atatatcgtt tggatataca catcaatcta
     2461 cccgcatttc tctttcctat ggccgacaaa gagaaggttt actgtgtgta ggaggtattt
     2521 gccgatatgt acccgcttat accggtttcg gactgacact ttcgagcaat ttttaaatcg
     2581 ttttgaaatg tttgaagaat cgaaatatct agaaaatttt ttcaaaacga atattttcaa
     2641 tcaacataat aacaacttat agaacaatat gaaaaaacac cacccgactt cagtcataat
     2701 tattacatgg atttttacga tgatgcaaat tgcctgcgat aaagtggatg ctccctacct
     2761 cgaacatatt caaacatatc ttgatacggc acaaacatcg gatacttcct ataagagaat
     2821 cgcgctatta gaagatttta ccggtcacaa atgtgtgaac tgcccccaag ccgcacaact
     2881 ggcaaaaaat ttacagttgg ctcataaagg tcagattgtc gtggtggaaa ttcatgcagg
     2941 tttttttgca gttcccgatc aatcagggct ctatattgct gactatcgct gtacggaagg
     3001 aacttcattg aacgattatt tcaacatcat gcaatatcct accggcctgg taaaccgaaa
     3061 agcttacgaa ggaaagataa gattgcttcc cacaagctgg gaatcggcca tactggcgga
     3121 gcttaagcaa gaaccattgg ccttcatcca actcgaaaac acatttaacc ctcaaaatcg
     3181 caatcttcga attgaaactg acattaaaat tttacaaaac ctcgaaggag aatataccta
     3241 tacgctttat ctaatcgaaa accatctcat ttcgtctcaa aaaaacaaca attctttcgt
     3301 tggtcctgtg cccgatataa ccgattatgt ccacaatcat gtacttcgga cttccattaa
     3361 tggaatttgg ggcgaaacct tacatcctgg gaatggattc gttaaagatg aaaccataca
     3421 attgattcac aattttatta tgagtgagaa ttggaatgcc agtcaatgcc acgtggttgc
     3481 ccttatgagc aaggtgggaa ccaatgaagt gattcaggct gctgaagaaa gcattgaata
     3541 gcaaaatcct cgagaatatt aacaaattga ataatagcaa tctatgaata gaaaatttct
     3601 tttatccttg cttggaattt ttttcttttt gacgcagctt cctcaagatt acgcccaggt
     3661 gctcgatcca gtaaagtgga gtttttcgtc ccaaaaaata aattccgatg aatatgagtt
     3721 aatttttact gctaaaatag acaagaactg gcatctctat tctcaatatg ttccagaagg
     3781 agggcctgtt cctaccaatt ttatcttcga accttcttcc aattttaaat taataggaaa
     3841 aacaaccgag ccaagacccc ataagatgta cgaccaaaat ttcgaaatgg aagtacagta
     3901 tttcgacggg caagccatat tcaaacaaag aatcaaatca ctcactcagc agagctttaa
     3961 tgttagagga acggtggagt tcatggcatg cgatgattct cgttgcattc ctcctgccga
     4021 agtggatttc gattttgcca tttctccttc tgctgaaccg gacaaagcag ttaccggcgt
     4081 tcagaaaact gaaacggcat atcagttaaa taccgattcg tcttttgggg agatatcaga
     4141 agaaaacatt gcaaccagtg ctaacgatag tgtcgcattg aataccagcc ttggaggcga
     4201 acagaaggaa aggcattcga acttatgggc ctttttctgt ttagcttttc ttggaggatt
     4261 gattgccatt ttcactcctt gcgtttttcc aatgatccct atgactgtta gctttttcat
     4321 gcatgacgaa gaaaacaaga agcaagctag acggcaggcg cttatttatg gtctgagcat
     4381 tatagttatt tatacagtta taggcacttt agtggctttg accttaggac cgaattttgc
     4441 aaactttctg agtactcatt ggttacccaa tctgttcttt ttcgtcatct ttatggtatt
     4501 tgccgcttcc ttttttggag ctttcgatct cactcttccc cattggatgg caaataaagc
     4561 cgatcaaaat gcggaaaaag gagggatttt gggtccattt ttcatggctt tcacccttgt
     4621 attggtttcc ttttcatgca cagctcctat tgtagggacc atccttgtag cttctgcaag
     4681 cggtgaaatc ctcaagccca tcatcggtat ggcaggcttt ggtttagcct ttgctttccc
     4741 ttttaccttg tttgctttat tcccctcctg gctgggtact ttaccaaaat ctggcggatg
     4801 gttgaattcg gtgaaagttg tcttgggttt tatcgaagtg gcgtttgggt tgaaattcct
     4861 gagcgttgcc gatcaaactt atcactgggg tatcctcgac cgcgaaatct atatcgccat
     4921 ctggatcgta gtattcacat taatgggttt ctatctcttg ggtaaaatcc gtttcaaaca
     4981 tgacgacgaa gtccatcata ttggagtatt tcgcttaacg cttgtaattg caactttctc
     5041 gttcgtagtc tatctattgc ccggcatgtg gggtgcacca ctcaaagccc tttcgggcta
     5101 tttgccgcct cagtcatcac acgacttcga ccttaaccag attattcgag aaaatgtaag
     5161 cgccaacctg aatatattac cccaacaaag taatccttca gtccccactg aacaatgcga
     5221 aaatcccaag tatgccgata ttttccattt accgcatgga ttgaaaggat atttcgatta
     5281 tcaacaagga ctagcttgtg caaaacaatt gaacaaaccc atctttatcg attttacggg
     5341 acatggctgc gtaaattgcc gggaaatgga ggccagggtt tggtccgatc caaaagtctt
     5401 gcaaagatta agaaacgact tcgtgattat tgctttatat gtggatgaca aaacacctat
     5461 acctgaaaat gaatgggtta tctcgtcata tgacggcaag gtgaaaaaaa cgattggtaa
     5521 aaaatacgcc gattttcaaa tttcgaaatt caatgtaaac gctcagcctt attatgttat
     5581 tctcgacaca aatggtcaac ctctgataca gcctaaggct tacgatctaa atatcgacaa
     5641 tttcattcaa tttctcgatg caggcaagca ggccttctat cgaaaagact aacctattgt
     5701 cagttttatt atcaaactct gattattttc tcgataatca gagtttttct attattctcg
     5761 aacagaaagc tcgttgggct ctctccgatt atctatagat ttagtgtcga ttttctgcgg
     5821 tagatattaa aaaactccga ttttcgaccg gagaattcaa gtgaccacgc tgggattcga
     5881 acccaggacc ccatccttaa aagggatgtg ctctgcctgc tgagctacgt agtcgtgagc
     5941 actgttttct ctttcgtttt cgggcggcaa aagtagctat ttttattaag ttatcaaaat
     6001 ttgtggattg ttttatttaa aattcaaaaa ggattgacgg aaaagatcaa aagtcaatag
     6061 gaccctttcc ttctctcacg attatgattt cattgctcgt acaatccagc accgtcgaag
     6121 gtacattgtc gccatagccg ccatctacca caatatccac caatgtttta tatttttcat
     6181 aaatcaactc gggttcggtg gtgtattcca aaatttcgtc ctcatcgcgg atcgaggtgt
     6241 tggctatcgg gttgcctaat tcacgcacaa tggcacgagc aatctcattg tcaggaactc
     6301 ttattcctat ggttttttta cgactctgga agatccgcgg aacattgcta ttggctttga
     6361 gaataaaggt gtaaggcccc ggcaaaagac gcttcattac cttgaaaata tgattgggaa
     6421 taggaagaca atactctgat atttgactta agtcatggca aataatagaa aactcggcct
     6481 tttcaacctt tattcccttg attctggcca ttctttccag tgctttcggg ctgtaaatat
     6541 tggc
//
LOCUS       DAORPD010000003         6264 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_2275_length_6264_cov_2.003382, whole genome shotgun sequence.
ACCESSION   DAORPD010000003 DAORPD010000000
VERSION     DAORPD010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6264
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2275_length_6264_cov_2.003382"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /gene="tgt"
                     /locus_tag="PK976_00080"
     CDS             <1..807
                     /gene="tgt"
                     /locus_tag="PK976_00080"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002560222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HRC66316.1"
                     /translation="PEGVVFQSHIDGSRHLFTPERVIDIQRIIGADILMAFDECTPYP
                     CSYDYASKSLQLTHSWLDRCLKRFNETQPEYHYDQSLFPIVQGSVYEDLRLQSAEYIA
                     SKNAPGNAIGGLSVGEPAEIMYQLTEKVCQILPSDKPRYLMGVGTPANILESIALGID
                     LFDCVLPTRNGRNGQLFTLEGIINIKNEKWKNDFSQIDQEGTSFVDHLYSRAYLRHLF
                     VTGEILGPMVASLHNLRFYHWLMEESRKHIMKGDFGEWKNLMIPRLSQRL"
     gene            832..1941
                     /locus_tag="PK976_00085"
     CDS             832..1941
                     /locus_tag="PK976_00085"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HRC66317.1"
                     /translation="MRKNFISFRLTVIDRYIIRKFLGTFFYSIGLLIIIVVVFDVSEK
                     IDDFLTRRAPLRAIIFDYYLNFIPYFINLFSSLFTFIAVIFFTSRLAERSEIIAMLSS
                     GINFFRLLKPYLISATFLVILTLYFSNFLIPHTNIKLRDFEKQYIKNPLRSKSLNLHL
                     QIEPGVFVYFESWDNARKNGYRFTIERIENSKLSYKLMSDQIKWDSIKSKWHIINYTE
                     RFIDTATFEQSLVFGSQKDTTLNLRPTEIIVDKEDMKIMNFNELKAFIEREKLKGSST
                     IKYYETEMHKRIAEPISAIILTLIGMALSSRKVRGGTGMHLGIGIGITFTYILFMQIT
                     TQFSVYGNMAPMLATWLPNIFFGIIAIWLLLKAPK"
     gene            complement(2014..2637)
                     /locus_tag="PK976_00090"
     CDS             complement(2014..2637)
                     /locus_tag="PK976_00090"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRC66318.1"
                     /translation="MKPQGKASREEILEAARKVFIENGYDGARMQEIADEAGVNKALI
                     HYYFRSKEELFQEVFLEAFIKVVPSVMVILNSDEPLFKKIEQFVSKYLDTLNENPLIP
                     GFILHELAHGRQHIDALIRGTGLNPQFFINQIYREIDAGKIKNCDPTQLIINILSLCI
                     FPFVAQPILKGVFFRNREDDYARFMLERKSDVVRFIIASIRKQQEES"
     gene            complement(2777..3874)
                     /locus_tag="PK976_00095"
     CDS             complement(2777..3874)
                     /locus_tag="PK976_00095"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC66319.1"
                     /translation="MRKILLSVLLMSLGGWLSAQSLQLKTLDGKLIANDATLYWGGLP
                     TEYMVARILVSNTSTEAKNVFVKKYEEFAAKDSTATFCWGACFPWFVNVSPEGIVIDA
                     GGTSTDFTADYDAGGVLGHTRLKFTFFVENNPNDSVSFRVEFSPSYLSLRNTEDSIPT
                     ASVLNVVGSYTEVIQYDLILTNHSKETKNVSMHKIHLSVIEGTEMYFCWGDCYPPDVF
                     ELPEPMVMAAGESSQGLSVDYDPKSHSGTSTITYVFYDFDHPADSVWVDFAFDGQAAG
                     IDYLASDILKISYRAFSHELFIEWENEISYGSKNLQVIDMSGKVVFTKTLSSNSRQQT
                     ITLPYLSQGIYMIVINLPDGMVYRNKFLISR"
     gene            complement(3977..4771)
                     /gene="pgeF"
                     /locus_tag="PK976_00100"
     CDS             complement(3977..4771)
                     /gene="pgeF"
                     /locus_tag="PK976_00100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HRC66320.1"
                     /translation="MLIDKDEPICCYFENLSRFDGEILHFVSTRRFLKDQQFSISFNG
                     SLSKDRVRVNRKVLFSKFGLDVSSSVFTTQVHGNKVERISIKEKGRGVNEVLKTCLGS
                     DGMITQHQEICLVIQMADCVPLLFFDPIKKVIATAHAGWRGTVMKIGAKVIEEFRNVY
                     DSDPADILAGIGPSIGPCCYEVGNEVISKVEEAFRDTKGILLNHPKFSRPVFDLWEAN
                     RRILIEKGINPRNIEMANLCTRCHSDMFFSARAGDEGRFGSFIMLK"
     gene            5201..>6264
                     /locus_tag="PK976_00105"
     CDS             5201..>6264
                     /locus_tag="PK976_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66321.1"
                     /translation="MSKILGLDLGVNSIGWAVVDDEKASIIDCGVRIFPEGVENLGQG
                     EKETSKNAQRREKRQLRRHYYRKRLSKIKLLETLIPLKMCPLSLNSLKKWKNWNNELK
                     SDGRKFPNDPEFIEWLKMNPYALRKKALEEDLSLEQLGRIFYHFIQRRGFYSSRKGKD
                     SGKIYQGENNTIGILETEKEIGYNKTLGTTLYEMILPEHQTYIYRTDENRRPLRARNR
                     YTLREMYVKEFHAIWEKQAPHLGLNDKTVTIFKSFHLSGNNDSERNKQRINKIYLQYG
                     IENVTITGKTIKVAQQISLKTLFGGRIEINDETHEILYRSQDSLLFWQRPLRSQKHLL
                     SKCTFEKRIMFDKTTNKWIET"
BASE COUNT         2078 a         1312 c         1112 g         1762 t
ORIGIN      
        1 ccagaaggtg tagtttttca atcgcatatc gacggttctc gccatctatt tactcccgaa
       61 agggttattg atattcagag aattattggt gccgatattc tcatggcttt tgacgaatgc
      121 actccttatc catgctccta cgattatgcc tcgaaaagct tgcaactaac acattcatgg
      181 ctcgaccgct gcttgaaaag atttaacgaa acccagcccg aataccatta cgaccaaagt
      241 ttatttccca ttgttcaagg aagtgtttac gaagaccttc gcctgcaatc ggccgaatac
      301 attgcttcga aaaatgctcc ggggaatgcc ataggaggct tgtcggtagg cgaacctgcc
      361 gaaataatgt atcaacttac cgaaaaagtc tgccaaattc taccatccga taagccacga
      421 tatttgatgg gagttggcac tccggccaat attctcgaaa gcatagcact gggcatcgac
      481 ctgttcgatt gtgtacttcc tacccgcaac ggccgcaatg gacagttatt tactttggag
      541 ggcatcatca acattaaaaa cgaaaaatgg aaaaacgatt ttagccaaat agaccaagaa
      601 ggcacttcct ttgtagatca cttgtattct cgcgcttatc tgcgtcatct tttcgtaact
      661 ggcgaaattc tcggccccat ggtcgcaagc cttcataacc ttcgctttta ccactggctg
      721 atggaagaat cgagaaaaca tatcatgaaa ggcgatttcg gagaatggaa aaacctgatg
      781 atcccccgat tgagtcaaag attgtaaatt cgcactttgt atcccatatt catgaggaaa
      841 aattttatat cattccgact caccgtaatc gatcgttata ttatccgaaa attcctcgga
      901 acatttttct attctattgg cctgctcatc attatcgtcg tcgttttcga tgtttccgaa
      961 aaaatcgatg atttcttaac ccgacgagca cctctgcgag ctatcatctt cgattattac
     1021 ctgaatttca ttccctattt tatcaatctt ttcagttcgc tcttcacctt tattgccgtc
     1081 atttttttta catcacgact tgccgaacgc tccgaaatca ttgcaatgct cagtagcgga
     1141 atcaatttct ttcgactgct caaaccatat ttgatctctg caacattcct cgtcattctt
     1201 acactctatt tctctaattt cctcattccc catacaaaca tcaagcttag agacttcgaa
     1261 aaacaatata tcaagaatcc tctccgaagc aaatccctca atcttcactt acaaatcgaa
     1321 cccggtgtat tcgtttactt cgaatcatgg gacaatgcca gaaaaaacgg ttaccgcttt
     1381 accatcgaaa gaatagaaaa cagtaaactc agctacaaac tcatgagcga tcagataaaa
     1441 tgggattcaa taaaatcgaa atggcacatc atcaattaca ccgaacgctt tatcgataca
     1501 gctacctttg agcaaagcct ggtatttgga agccaaaagg acactactct caatcttcgc
     1561 cccacagaaa tcatagtgga caaggaagac atgaagataa tgaatttcaa tgagttgaag
     1621 gcgttcatcg agagagaaaa actcaaagga agcagtacga taaaatatta cgagacggag
     1681 atgcacaaac gtatagccga acctatatcg gccatcatac ttacattgat tggtatggca
     1741 ctgtcgagca gaaaagttag aggaggaaca ggtatgcacc tgggtatcgg tattggcatt
     1801 acttttactt atattctgtt catgcagatc actactcaat tctcggttta tggaaatatg
     1861 gcgcccatgc tggctacctg gcttcctaat atcttttttg gaattatagc tatctggtta
     1921 ttgctcaaag cccctaaata ataacaggtt tttatcgaca actcactccc aaagaataac
     1981 tctttagagg aaacatcggc caaaaaactc aaatcaactt tcttcctgct gtttacggat
     2041 ggaggcaatg ataaacctga caacatccga cttgcgttcc agcataaatc gcgcgtaatc
     2101 atcttcccgg ttacgaaaga atacaccctt caaaataggc tgagcaacaa aaggaaaaat
     2161 acacaaggaa aggatattaa tgatgagctg ggttggatcg cagtttttga ttttccccgc
     2221 gtcaatttct ctgtatattt gattgataaa aaactgagga tttagtcctg tccctctgat
     2281 caaggcatcg atatgctggc ggccatgagc aagttcgtga agtataaatc ccggaatcaa
     2341 tggattttca ttcaaagtgt cgagatattt cgaaacaaac tgttcgattt tcttgaacaa
     2401 tggctcgtcg gagtttaaaa tcaccatcac cgagggaact accttaataa aggcctctaa
     2461 aaaaacttcc tgaaaaagtt cttccttcga ccgaaaataa taatgaataa gggctttatt
     2521 tacccctgcc tcatcggcaa tttcctgcat gcgggcccca tcgtaaccat tctctataaa
     2581 aaccttgcgg gcggcttcca aaatctcttc ccgactcgct tttccctgtg gtttcatgtt
     2641 ttataccaat caaaatttat atcctaaagc aaaaataatt tttttgaaag aagtttcctt
     2701 aaaaaaacaa aaaaactgcc gaatatttaa aaatttaaaa ctccggcagc cttaaaaata
     2761 tacgataaaa aattatttaa cgactaataa ggaatttatt tcgataaacc attccgtctg
     2821 gcaaatttat tacaatcata tatatgccct gagaaagata aggaagtgta atagtttgct
     2881 gcctgctatt gctggataag gtttttgtaa aaacaacctt tccgctcata tcaattactt
     2941 gaagattttt actgccatat gaaatttcgt tttcccattc gatgaaaagt tcatgactaa
     3001 aagctctgta agaaatcttc aaaatatccg aggcgagata atcgattccg gcggcctgac
     3061 catcgaaggc aaaatcaacc caaaccgaat cagcgggatg atcaaaatca taaaatacat
     3121 aggttatggt tgatgttccc gaatgcgatt ttgggtcata atccacactc agcccttgag
     3181 atgattctcc agcagccata accatgggtt cgggaagttc aaatacatcg ggtggataac
     3241 aatctcccca acaaaaatac atctcagtac cctcaatcac cgacagatga atcttgtgca
     3301 tgctcacatt cttcgtttct ttgctgtgat tggtcaatat caaatcatat tgtatcacct
     3361 ccgtatatga gcccacaaca ttcagaacag aagcagtagg aatggaatct tctgtgtttc
     3421 tcagactaag gtaggaaggc gaaaattcta cacgaaacga aacgctgtcg ttgggattat
     3481 tttccacaaa aaaagtaaac ttcagccgtg tatgacccag tacgccaccg gcatcataat
     3541 cggccgtaaa atcagtagaa gtgcctccag catcaatcac aatgccttcg ggacttacgt
     3601 tgacaaacca tgggaaacaa gctccccagc aaaaagttgc agtagaatcc ttggcggcaa
     3661 attcctcata cttcttcacg aaaacattct ttgcctcggt agaagtattg cttaccaaaa
     3721 ttctggccac catatattcg gtgggcaatc caccccaata aagagtagca tcattggcaa
     3781 tcaactttcc atccaaggtt ttaagctgaa gactctgagc tgataaccag cctcccaaag
     3841 acatcaataa tacagaaagt aaaatttttc tcatcgctta ttaatttggg tttgtaaaat
     3901 gttggttcat gttgcaaaaa tagagatttt catcactcgg tctaaacatt ctgctgtttt
     3961 cccttgattg aaatatttat ttcaacataa tgaaagaacc aaaacggcct tcatcgccag
     4021 cacgagccga gaaaaacata tctgaatgac aacgcgtgca aaggttcgcc atttcgatat
     4081 tgcggggatt tatgcctttt tctatcaaaa ttcttcgatt ggcttcccat aagtcgaata
     4141 cgggccgaga aaatttggga tgattcagca aaatgccttt ggtatcacga aaggcttctt
     4201 ccactttgct tatgacctca ttccctactt catagcaaca tggcccgatg gatggcccaa
     4261 tgcctgccaa aatatctgcc gggtccgaat cgtaaacatt acgaaactct tcgataactt
     4321 ttgcgccaat tttcattaca gttcctctcc aaccagcgtg ggcagtagct ataacctttt
     4381 ttataggatc aaaaaataac aggggaacgc aatcagccat ttgaataacc agacaaatct
     4441 cctgatgttg cgttatcatg ccatcgctgc caaggcaggt ttttaaaact tcgttgacac
     4501 ccctgccctt ctcttttatt gaaatgcgct ccaccttgtt tccatgaacc tgtgtggtaa
     4561 acacagaact actcacatcg agtccaaatt tcgagaaaag gacttttcgg tttactctta
     4621 ctctatcttt cgacaaagaa ccattgaagg aaatggaaaa ctgctgatct tttagaaacc
     4681 ttcgtgtgga aacaaaatgc agaatttccc cgtcaaatct cgaaagattc tcgaaataac
     4741 agcaaattgg ttcatcctta tctattaaca tctaaccctg aatttctgca aaacaaagag
     4801 attccggaca attttgcaaa aaaacatctt tgacgaagac atttgtgcta cctttataga
     4861 aatttaataa atgagctacg acacgaagat tactgatttt attaaatcaa taaatctccg
     4921 acttaaccgg taggatcaaa tggaaaggat aaagtcacaa actaaaaaaa cccctaccga
     4981 agcaggggca taggactaag tccttcggca tatagccttg ttgtgataag ataagattgg
     5041 aataatcttc gtcaacaaag atacaacttt tttattatgc aacactttca taaaaaatat
     5101 ttaattttaa cccatcttct aaggaaaaca aaggtattaa ctggacaaac cacactcaga
     5161 aatctaaaat taaatctaat aatttaaaaa ttaatcaatt atgagcaaaa tccttggact
     5221 cgatttagga gtcaatagca ttggatgggc agttgtagat gatgaaaaag cgtcaattat
     5281 tgactgcggg gttagaattt tccctgaagg agtagaaaat ttaggacagg gggaaaaaga
     5341 gacttcaaaa aatgcacaga gaagagaaaa aagacaactc aggagacatt attacagaaa
     5401 aagattaagt aagatcaaat tacttgagac attgatccct cttaaaatgt gccccttatc
     5461 attaaactca ttgaaaaaat ggaaaaattg gaacaatgaa ttgaaatcgg atggaagaaa
     5521 gtttccaaat gatcctgagt ttatagaatg gttgaaaatg aatccttacg cattgcggaa
     5581 aaaagcatta gaagaagatt taagcttaga acaactcgga agaatctttt atcattttat
     5641 tcagagaagg ggtttttaca gtagtcgaaa aggaaaagat tcaggaaaga tttatcaagg
     5701 ggaaaataat accattggta tactcgagac cgagaaagaa attggttata ataaaactct
     5761 tggaactact ttgtatgaaa tgattcttcc cgagcatcag acttatatct acagaactga
     5821 tgagaataga cgtccgctaa gagcccgtaa ccgatatacc ttaagagaaa tgtatgttaa
     5881 ggaatttcat gctatttggg aaaaacaagc tccacattta ggattaaatg ataaaactgt
     5941 tacgatattc aaatcctttc atctttctgg gaataatgat tctgaaagaa ataagcaaag
     6001 aatcaataaa atttacttgc aatatggcat tgagaatgtt actattactg ggaaaaccat
     6061 aaaagttgca caacagatct ccttaaaaac tctttttggg ggacgaatcg aaattaatga
     6121 cgaaactcat gaaatattat atagaagtca ggatagtctt ttattctggc aacggccatt
     6181 aagatcacaa aaacatcttt tgtcaaaatg tactttcgaa aagagaataa tgtttgataa
     6241 aactaccaac aaatggatag aaac
//
LOCUS       DAORPD010000004         6108 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_2346_length_6108_cov_2.065422, whole genome shotgun sequence.
ACCESSION   DAORPD010000004 DAORPD010000000
VERSION     DAORPD010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6108
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2346_length_6108_cov_2.065422"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..1553)
                     /locus_tag="PK976_00110"
     CDS             complement(135..1553)
                     /locus_tag="PK976_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005635929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HRC66322.1"
                     /translation="MPSTNDPQKTIASSVTISGTGLHTGEFVTLTLKPAPINYGYKFV
                     RSDIEGYPQIEALVDYVNNTDRSTNLQKDNIHISTVEHLLAAIYGSGIDNILIEMDGN
                     EVPILDGSSAPWVDLIHKARLINQKAERVYFELKHNIIHTQSDRKSEIIAIPSDIQRF
                     SAMIQFNSKVLDTQFATLDRIDDFAKEIAPCRTFVFLHELEQLIQHNLIKGGDINNAI
                     VLVDRILSEEELKRLAIYFDRPEVKVLEEGVLNNLELHFMNEPARHKLLDLLGDIALT
                     GFRFKAHVLATRPGHVVNVEFARKMREAYLKEHRKTEHTPQYDPNKPPLLDINDIKKL
                     LPHRPPFLLIDKILEMTDSHVVGLKNVTMNESFFAGHFPDEPVMPGVIQIEAMAQTGG
                     VLVMSTVPDPENYLTYFMKINEVKFRHKVVPGDTLIFSLELISPIRRGLCHMRGKAFV
                     GDKVVMEAEMLAQISKKPTPSN"
     gene            complement(1616..2662)
                     /gene="lpxD"
                     /locus_tag="PK976_00115"
     CDS             complement(1616..2662)
                     /gene="lpxD"
                     /locus_tag="PK976_00115"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HRC66323.1"
                     /translation="MEFTAREIAAMLNADIEGDPETRINNLAKIEESNPGTLTFLANP
                     KYIPYIYTTKASLVLVGKNFEPEHPIQSTLLRVDNPYVAFAFLLEKFNQIFLNRQGIS
                     PQAFIHPSAHIGKNVYIGEFSYIGEDTEIGDEAKIYPLTYVGDHCEIGEKTTLFSGVK
                     VYSMNIIGRECTLHSGVVIGADGFGFAPQGNDYQKVPQIGNVIIKDHVEIGANTTIDR
                     ATLGSTIIHKGVKLDNLIQVAHNVEIGENTVIASQTGISGSTKIGANCMIGGQVGIIG
                     HISIADEVKIAAQSGIGGSITQKGAIVQGSPAFEYKKYQKSYVHFRNLHQLYEKINQL
                     EERLKELEQLNKDE"
     gene            2941..3270
                     /locus_tag="PK976_00120"
     CDS             2941..3270
                     /locus_tag="PK976_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HRC66324.1"
                     /translation="MTLNEAQQKVDQWIRTIGVNYFDELTNLALLMEEVGEVARIMAR
                     KYGQQSAKPEDVSTTLADEFADVLFVLICLANQTGVNLTEAFRSNLEKKTTRDALRHQ
                     NNPKLKK"
     gene            3344..5836
                     /locus_tag="PK976_00125"
     CDS             3344..5836
                     /locus_tag="PK976_00125"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HRC66325.1"
                     /translation="MLKSLCLSMNFLRSYILRSFIKLIPRFLAACFLLNIPMVYAQVL
                     YSISGKVVDAHNGLPLAFVNIQVNDKMVGTVSDIDGNFNFSSSQPIEKLRFSYIGYEP
                     LVLEQSSLKQKIIVKLRKTEIELKEVTVYPGQNPALRIIRNALNNRQRNNIENLPGFS
                     YNAYEKTVFSIKVDSLLLSDTNSLDTNLRQLKEFVNRQHLFIIENYIERQYKSPRKNS
                     EKVVATKISGLKDPIFVFLLSRLQSSTFYEDVFSIGGQNYINPLAENSIDKYFYQLED
                     TIISGQDTTYIIFYRPWKGKNFDGLKGVLHINSNGWAISSVTAEPYQKDENFEIKVQQ
                     LYAQDSNGIWFPRQLNMDLIMNIVQVNAGNKQTPFRIIASGKTYIENFRVIESSVKNI
                     SSHIEIDVIPEAAYRDEDYWQRVRAVPIDEREKETYRVIDSLGAAQNLDKFTYTLDAF
                     MKGKIAIKKVNLDLEKIMHYNAHEGFYAGLGLETNDLFSRRWQTGAYWGYGFKDKTAK
                     WGTFLRLNLSSLHDSYFRYDYRFDKQESGATEFTGRNLSEFDPYTFRNFQINRMDGLN
                     AHQLIFSTRIFEYTNVWLAMSIAQMNPAYDYRFQYQEFAKNETFGFSELSASFRWAYE
                     EKFIRTTHGKVSLGTKFPIVWFQYSKGLDFLPGGDFDYFRSLFDITYSFYTPFVGKTS
                     FRLNGGMSSSNLPAGKLFNARANHADFTLFSPGSFVTMRMNEFYSDRFIALFVQHNFG
                     HLLFKSGKFQPDLEIFSSALFGSLKHPEYHEGISMKVAEEGYFESGLLIHRLIDFGFY
                     NLGLGAAWRYGTYSLSKLSDNLALMLSLRFAM"
BASE COUNT         1824 a         1207 c         1188 g         1889 t
ORIGIN      
        1 gatggatttt agcctcggga tgaatactcg taagtggttg atgcattttt tgcggattaa
       61 tttgcaaatg agtccgggat tactatattc attttaaaca acttaaataa cttaaacaac
      121 ttcgacaaaa ataattaatt cgaaggagtg ggttttttgg aaatctgtgc aagcatctcg
      181 gcttccatga ctactttgtc acctacaaaa gctttccctc tcatgtggca taaaccccgc
      241 cgtattggcg aaataagttc gagagaaaaa ataagtgtat caccgggaac tactttatgc
      301 ctgaatttta cctcattgat cttcatgaag taagtgagat aattttcagg gtcgggcaca
      361 gtactcatta ctaaaacgcc tcctgtttga gccatggctt cgatttgtat gactccaggc
      421 atgacgggct cgtcggggaa atgtccggca aaaaacgact cattcatggt tacattcttt
      481 aagccaacga catgagaatc agtcatttct aatattttat caatcaataa aaaaggaggc
      541 ctatggggta aaagtttttt aatgtcgtta atgtcgagca aaggcggctt attgggatca
      601 tattgaggag tatgttccgt ttttcggtgc tctttcaagt aagcttctct cattttgcgc
      661 gcaaattcca cattaacgac atgtcccgga cgagtagcaa gtacatgggc cttgaatctg
      721 aaacctgtca atgctatatc tcccaaaaga tccaggagtt tatggcgagc aggctcattc
      781 ataaaatgta actcgagatt gttcaaaacg ccttcttcga gtaccttcac ttcaggcctg
      841 tcaaaataaa tggcaagcct tttcagttct tcctctgaca gaatcctgtc aaccaatacg
      901 atggcattgt ttatatcgcc acctttaata agattatgct ggattaactg ttccagttcg
      961 tgcagaaaga cgaaggtacg gcagggagct atttcttttg caaaatcgtc tatcctgtcg
     1021 agagtggcaa attgtgtatc cagaaccttc gagttgaatt gaatcattgc cgagaaacgt
     1081 tgaatatcag aaggaatagc aatgatttcc gactttcggt cggattgggt atgaattata
     1141 ttgtgtttga gttcgaaata tacacgttcg gctttctgat ttatcaaacg ggctttatga
     1201 atcaaatcga cccagggagc cgaacttcca tcgagaatgg gcacttcatt cccatccatt
     1261 tctattaata tattgtcgat tccacttccg tatatagctg caagcaaatg ttcaactgta
     1321 ctgatgtgta tattatcttt ttgcaaattg gtgcttctgt cggtattgtt tacgtagtca
     1381 accaaagctt ctatctgtgg atacccttct atatcgctac gaacgaattt atatccataa
     1441 tttatagggg cgggtttcag agttagggta acaaattccc ccgtatgcag gccggtgccc
     1501 gagatagtta ctgaagaggc tatcgttttt tgtggatcat ttgtcgacgg catttcctta
     1561 tgagattaaa taatttgcaa aggtagaaaa tgtgcaaaaa ctgaagaagt cgaagctatt
     1621 catctttatt gagctgctca agctctttca gcctttcttc taactggttt atcttttcat
     1681 agagttgatg gaggtttctg aaatgcacat aagatttttg gtatttttta tattcaaaag
     1741 caggacttcc ttgcacaatg gctcctttct gggtgataga acctcctatc cccgattggg
     1801 ctgcaatttt tacttcatcg gcaatactga tgtggccaat gatcccaact tgacctccta
     1861 tcatacaatt agctccgatt ttcgtcgaac ccgaaattcc agtttgagag gctattacag
     1921 tgttttcgcc aatttcgaca ttatgagcca cttgtattaa attgtcgagc tttacaccct
     1981 tatgaataat cgtacttcct aaagttgccc ggtcaatagt agtattggca ccaatttcca
     2041 cgtggtcttt aatgattacg ttgccgattt gaggtacttt ctgataatcg ttgccctgag
     2101 gagcaaagcc aaaaccatcg gccccaatca ccacgcccga atgcaatgtg cactccctac
     2161 caataatatt catgctgtaa accttgactc ccgaaaacaa tgtagttttt tcgcctattt
     2221 cgcaatgatc acctacgtaa gtcaaaggat aaatctttgc ctcatcgcct atttcggtat
     2281 cttcgccaat ataggagaat tcaccaatat aaacgttctt gccaatatga gcgctgggat
     2341 gtataaatgc ctgaggcgat attccctgcc tgttgagaaa aatctgattg aatttttcca
     2401 gtaaaaaggc aaaagctaca taaggattgt caactcgcag aagagtggat tgaatggggt
     2461 gttcaggctc gaaatttttg cctacaagta ccaatgaagc tttggtggta tatatataag
     2521 gtatatattt tgggttagct aaaaaagtta atgtacccgg gttcgactct tcgatcttcg
     2581 ccagattatt gatccgcgtt tctggatctc cttcgatatc ggcattaagc atggcagcta
     2641 tttctcgggc agtaaattcc atttttctat cgtattatga gagtacaaaa gtagatgatt
     2701 gtcgtaaata atgaaatttc acatttctac taattttata ccgaaatctc acaataagac
     2761 aatcaaggtg aaaaattttt attctttcct tatgaaattt taacaaagaa ctatatttca
     2821 acacaagaac acctctggga agatcttgtt gttagcatta aataacaaat tatgccacag
     2881 ttttattata aaatcattta ttttgaagtc ctgaatcata aaggtaaaat ttaaagaaat
     2941 atgacgctta acgaagctca acaaaaggta gatcaatgga taagaaccat tggagtaaat
     3001 tatttcgatg aattgaccaa tctggcgtta ttgatggaag aagtgggaga agtagctcgt
     3061 attatggcga gaaaatatgg ccagcagtcc gccaagcctg aagatgtatc caccactctg
     3121 gccgatgagt ttgccgatgt tttattcgtg ttgatatgtc tggccaacca aaccggagta
     3181 aaccttacag aagcatttcg ttcgaatctc gaaaagaaaa ctacacgtga cgctttacgc
     3241 catcaaaata atcccaaact gaagaaataa taacctattc atttattcta actctggatg
     3301 aaaatattcg tttttaattt ttcataaaaa tcgaaatcga ggaatgttga aaagcctttg
     3361 tttgtctatg aattttctgc gttcttatat ccttagaagc tttattaaac tgataccccg
     3421 ctttttagcg gcctgtttcc tcttgaacat accaatggta tatgcgcaag ttctctattc
     3481 tatttcggga aaagtagtgg atgctcacaa tgggttgccc ctggcctttg taaatattca
     3541 ggtaaacgac aaaatggtgg gcacagtgag cgacattgat gggaatttta acttttcctc
     3601 atcacaacct atcgagaaat tacgattttc atatatcggc tatgaaccac tcgtattaga
     3661 acaatcgtca ttaaagcaaa aaattatcgt taagctcaga aaaacagaaa tagaattaaa
     3721 agaggttacc gtttatccag gacaaaatcc agcactacgg atcatccgga atgccttgaa
     3781 caatcgccaa cgcaacaata tcgaaaattt accgggtttt tcgtataatg cttatgagaa
     3841 gacagtattc agcatcaaag ttgattcact tttgctttca gatacaaatt cgctggatac
     3901 taacctgagg caactaaagg aattcgtaaa ccgacagcat ctatttatta tcgaaaacta
     3961 tatcgaacgc cagtataaat caccacgtaa gaattccgaa aaagtagttg ccactaaaat
     4021 ttcgggtttg aaagacccca tattcgtatt tctacttagc cggttgcaat cgagtacatt
     4081 ttatgaagac gtttttagca ttggtggcca aaactatatt aaccctttgg ctgagaacag
     4141 catagataaa tatttttatc aactcgaaga cacaattatc agtggtcagg atactactta
     4201 cattattttt taccgcccat ggaagggaaa gaattttgat ggattgaaag gggttttgca
     4261 tatcaatagc aacggttggg caatttcgtc ggttactgcc gaaccctacc aaaaagacga
     4321 aaacttcgag ataaaggttc aacaactata tgcacaggac tcgaatggaa tatggtttcc
     4381 gcgccagcta aacatggatt tgatcatgaa tattgttcag gtgaatgctg gtaataaaca
     4441 gacgccgttt cgaatcatcg caagcggaaa gacttatata gagaatttca gagttatcga
     4501 aagctcagtg aagaacattt cctcgcatat tgaaatagat gtaatacccg aagctgcgta
     4561 tagagacgag gattattggc agagagtaag agcagtaccc atcgacgagc gagaaaagga
     4621 aacctaccgt gtgatagata gcttaggcgc cgcccaaaat ctcgacaagt ttacctatac
     4681 gctcgatgct tttatgaaag gaaaaattgc tatcaaaaaa gtaaatcttg atctggaaaa
     4741 aatcatgcat tacaacgctc acgaaggatt ttatgccggt ttgggattag aaaccaacga
     4801 tctcttttcc cgtcgctggc aaaccggggc gtattggggt tatggattca aggacaaaac
     4861 agccaaatgg ggaacatttc ttcgacttaa tctttccagc ctgcatgatt cttattttcg
     4921 ctatgattat agattcgata agcaagaaag cggagcaacc gaatttacag gacggaattt
     4981 gtcggaattc gatccttata ccttccgaaa tttccagatt aacagaatgg atggcctcaa
     5041 tgcacatcaa ctcatctttt caacccggat attcgaatat actaatgtct ggttagccat
     5101 gtccatagca caaatgaacc ctgcctatga ttacagattt caataccagg aatttgctaa
     5161 aaacgaaaca tttggttttt ccgagctatc ggcctctttt cgctgggctt atgaggaaaa
     5221 attcattcgt accacacatg gtaaggtttc gctcggaaca aaatttccca tagtttggtt
     5281 ccaatatagt aaaggactcg actttttgcc aggaggagat ttcgattatt ttcgttccct
     5341 tttcgatata acttattctt tctatacccc tttcgtcggg aaaacctcct tccgcctgaa
     5401 tggtgggatg tcttcttcga atcttccggc aggtaaactc ttcaatgcgc gtgccaacca
     5461 tgccgatttt acgctgtttt cgcccggaag cttcgtaacc atgcgcatga atgaattcta
     5521 ttccgaccgt tttatagccc tattcgttca gcataacttt ggtcatctat tattcaaatc
     5581 gggaaaattt caacccgacc tggagatttt ttcttcggct ttgtttggaa gtcttaaaca
     5641 tccagaatat catgaaggca tttcgatgaa agttgctgaa gaagggtact tcgaatcagg
     5701 cctgcttatc caccgtctga tcgattttgg cttctataat ttaggattgg gagccgcttg
     5761 gcgttatgga acgtacagtt tatctaaact ctccgataat ttggctctaa tgctgtcatt
     5821 gcgctttgcc atgtaatcat tcgttgaatc catcctaata ttgtcgatat tgcttcattt
     5881 cgaagttcga catgggttct tgaaaataaa gttggtccct accaattctg aaggttttca
     5941 tgcttgccat aattaggaaa ttctatttcg aaaatactgg cgacctttca taacccaact
     6001 tgagcatttt accctgaaaa aacagggaaa aatagggttt ttgctatgat ttaaaatgtt
     6061 atgttactga ttattaaata tttaaatttt taataatttt tgtagtcc
//
LOCUS       DAORPD010000005         6019 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_2391_length_6019_cov_2.995808, whole genome shotgun sequence.
ACCESSION   DAORPD010000005 DAORPD010000000
VERSION     DAORPD010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6019
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2391_length_6019_cov_2.995808"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /gene="pyrI"
                     /locus_tag="PK976_00130"
     CDS             <1..426
                     /gene="pyrI"
                     /locus_tag="PK976_00130"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009127487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HRC66326.1"
                     /translation="IQNGTVIDHIPSHAVIQVMRILNLIQTDHQVLFGMNLDSKKYGN
                     KGIIKIRDKFFDDVEINKIALVAPTATLIEIRDFEVISKKQAEIPDIVEGIVKCFNPK
                     CITNYEQVRTRFRVIDKQNLRLLCHYCEKITKNENIEFI"
     gene            501..1013
                     /locus_tag="PK976_00135"
     CDS             501..1013
                     /locus_tag="PK976_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66327.1"
                     /translation="MPASLPDLPSLLWEVAMFCIILYPKLAILMKNSFGWLIAGFLFF
                     ELLMIQSCSKENNSSQYAYDLQPHIENLYSSFIYRYNTGYYTADTGYKLLLNPENKEF
                     EIHLTVQDYKSGWMKLSFFDVDQEIVSTDTLRFNYDSILQIPVSIDSLSLKSQIFTGI
                     IYFSLQPSFK"
     gene            1546..2043
                     /locus_tag="PK976_00140"
     CDS             1546..2043
                     /locus_tag="PK976_00140"
                     /inference="COORDINATES: protein motif:HMM:NF012307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HRC66328.1"
                     /translation="MPQGVKFVEITKPNGGLCKLCISTIIDRVIQQAIAQVLSPIYEK
                     TFSDYSYGFRPKRNDRQAVRKASEYVVMGREIVVDIDLKNFFDVINHDRLMYRLSETI
                     GDKVLLKLIRRYLRSGIMADGVLNQCTEGTLQGSPLSPLLSNIVLDEPDKELEKRGTL
                     FCSIC"
     gene            2183..2320
                     /locus_tag="PK976_00145"
     CDS             2183..2320
                     /locus_tag="PK976_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66329.1"
                     /translation="MGYGIEIDGTLTISGISIKRFKDKIHRLTQRNRGRSIAQHRLFE
                     S"
     gene            2394..2612
                     /locus_tag="PK976_00150"
     CDS             2394..2612
                     /locus_tag="PK976_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66330.1"
                     /translation="MDTSQIGMYQAQAMQTGLHPTAIFGTAMGEEVAKLDTCVFWERM
                     VAKVRHTQSPTGHGGQHGSRKLAYTVCH"
     gene            3122..4141
                     /locus_tag="PK976_00155"
     CDS             3122..4141
                     /locus_tag="PK976_00155"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRC66331.1"
                     /translation="MNDSQIETDLFDKFRNLRVLVVGDVMVDSYIWGRVERLSPEAPV
                     PIVDVLDRSNRLGGAANVAINIKSLGAEPILCSVIGKDSPSRTFVRLMKESGLDQDGI
                     LQSNNRITSTKYRIFGNNTQLLRIDEETNCPILPSDQSRLLQIIEFQVQTNHPDIIIF
                     QDYDKGVLTQGIIRDIMQLATEAKIKVCVDPKRINFWAYDDVLLFKPNLKELKEGLKM
                     NHIYQTDEFILKDAIIALQNKINAEIVLVTLSDEGMVIRYSVESEGFQYQSIPAHLRH
                     IADVSGAGDTVISVASLCLAAGFSPLLSAEIANVAGGLVCEEVGVVPINAKRLSEEIE
                     RLNLY"
     gene            4159..4854
                     /gene="ubiE"
                     /locus_tag="PK976_00160"
     CDS             4159..4854
                     /gene="ubiE"
                     /locus_tag="PK976_00160"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HRC66332.1"
                     /translation="MENHRSTQPILFDEIAPRYDFLNHLLSVGIDISWRRKLVNRLPE
                     NNQPYILDVATGTADLAIMAAKRIPCRIIGYDIADRMLEIGERKIAQNSLTHKIQLIR
                     GSVEDLPFADNSFDAVMVAFGVRNFKNLPKGLSEMYRVLKPDGISLILEFSIPENKIV
                     RNLYHFYFFRIFPLIGGWLSKNRKAYTYLPESVQQFPQGKHFERMLKGMGFRRTTFQS
                     LTFGIAILYEAIK"
     gene            4904..5680
                     /locus_tag="PK976_00165"
     CDS             4904..5680
                     /locus_tag="PK976_00165"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HRC66333.1"
                     /translation="MMRQIRKIHFLGFLFLLISVMVYSQEGPSVMNLPKFDRASRHFG
                     FYVSGNQMFFTIQPADKFQQKVWNRDQIPDLVADSARVIGIESSPVPGFSIGLVSNLR
                     LGEYFDLRFLPGLQFGERELHYSVMQFSHADSSIVNISKNINSTFIDFPLYVKYKSKR
                     LNNFRAFVTAGASYKIDLASSTKDKAENPSNVVVKLKKHDFTGDLGVGFDFYTVYFKF
                     STEIKMSYGINDMIYREGNIYTDSIKKLSAKMFQISFTFE"
     gene            5695..>6019
                     /locus_tag="PK976_00170"
     CDS             5695..>6019
                     /locus_tag="PK976_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66334.1"
                     /translation="MDGISNEELFSTLNDKGNLKKEIYQNTERAFELINQAARKISQI
                     SPMKDEHLRIEFNRISPFQSQLFFAGDALIFFMQTDMYEFSRDHDVMRTAYIRQDKTR
                     SYVGII"
BASE COUNT         1803 a         1118 c         1264 g         1834 t
ORIGIN      
        1 attcaaaatg gaacagtaat cgaccatatt ccctcccatg ctgttattca ggtaatgcgt
       61 atcctcaacc tcattcaaac cgaccatcag gtgctctttg gaatgaacct cgatagtaaa
      121 aagtatggca ataagggaat tatcaaaatt agagataaat ttttcgatga tgtggaaatc
      181 aataagatag cccttgtagc ccctactgct acccttatcg aaattcgtga ttttgaagtg
      241 atatcgaaga aacaagccga aatacccgat attgtcgaag gtatcgtcaa atgtttcaat
      301 ccgaaatgca ttaccaatta cgaacaagta cgaacacgct ttagggtgat cgataagcag
      361 aatttgcgtt tgctctgcca ttactgcgaa aagattacca aaaatgaaaa tatcgagttc
      421 atctgacctc tgcttgcctt cttctttgct catcttattg cagatcccaa acaaaggaag
      481 gaggatcatg ttaaccatga atgcctgcgt cgctacctga tcttccttcc cttctttggg
      541 aggttgccat gttttgcata attttgtatc ccaaattggc tatattgatg aaaaattcat
      601 tcgggtggtt gattgctggg ttccttttct tcgaattatt gatgattcaa tcctgttcga
      661 aagaaaataa ctcttcccaa tatgcttatg atcttcaacc acatatcgaa aatctatatt
      721 cttccttcat ttacaggtat aatactggat attacactgc cgatacaggt tacaaactgt
      781 tattgaatcc cgaaaacaaa gaattcgaga ttcatcttac agttcaggac tataaatcag
      841 ggtggatgaa attatctttc tttgatgtag atcaagagat tgtctctact gacaccttgc
      901 gtttcaatta tgactcgata ttgcaaattc cggtttcgat agattctctt agtttgaaat
      961 cacaaatttt taccggaatt atctattttt ctcttcaacc ttcattcaaa taatgaaatc
     1021 ttcgtcgaat atggataatt ctccgatttc ggatgaaatt taaaatatta agtaaattga
     1081 ttattaatca atatgttaaa tgagtatcaa gtctcttttt aagaaataaa ttgttgtttt
     1141 cggaatattc tattcccaaa gggtagccct cacatgtcat tttctcattg attttaagga
     1201 tatttattat tatggcacga tagttgtact ttttttagag ctgttgcagg ttaatacctg
     1261 gtaataccaa accaaactaa actctcttta cctactaatt gtttttgttg tttaagtaat
     1321 tttgattact tttttccggt gcgtagggat acgcgccggt tttttttatg acgggcatac
     1381 attactactt gcaagatgaa agttcttgta cacgccgagt tgggaggagg tgttagcgaa
     1441 tggaagggtg taagcgggat tgatgggatg ggagtaggag aatttgtcga atggtacacc
     1501 cgtaatgggg gcaccttacg tcactcctta ctgagtggca actatttgcc acaaggtgtc
     1561 aaattcgtag aaatcactaa gcctaacgga gggttatgca agttatgtat ttccacgatc
     1621 atcgaccgag tcatccaaca agcgatagcc caggttttaa gtccgatata cgagaagacg
     1681 ttttccgatt atagttatgg ttttcgaccc aaacgtaatg atcgacaggc tgtacgcaag
     1741 gcgagtgaat atgtagttat ggggagggaa attgttgtag atatcgactt aaagaacttc
     1801 tttgacgtta taaatcacga tcgactgatg taccgattaa gcgaaactat cggggacaag
     1861 gtactactta agctgatacg caggtatctt cgaagcggga tcatggcaga tggagtgttg
     1921 aaccaatgca ctgaaggtac tctgcaaggc agtccgctct cgccattact gtctaacata
     1981 gtgttggatg agccggataa ggaattggag aaaaggggga cactgttttg ttcgatatgc
     2041 tgacgattgt aatatttttg tatgcagcca tgaggcaggt gaaagggtat tgcggtcggt
     2101 aagcagtttt atcgaaggca aattaaagct caaggtaaat actgaaaaga gtaaagtttg
     2161 ccttagccga gaagccaaat ttttgggata cggcattgaa atagatggga cgctcaccat
     2221 tagcggaata agtatcaagc gtttcaagga caagatacac aggttaactc aacgtaatcg
     2281 agggcgaagc atagcccagc acaggttatt tgagagctga atcctgtatt acgaggctgg
     2341 ctgatgtatt acgaggcagc gaaatgcgcc tcttttgtac gtgaacttga gggatggata
     2401 cgtcgcaaat tgggatgtat caggctcaag caatgcaaac gggtttacat cctacagcaa
     2461 tttttggaac ggcaatgggt gaagaagtgg caaagttgga tacttgcgtt ttttgggaaa
     2521 ggatggtggc gaaagtcagg cacactcaaa gccccacagg ccatggggga cagcatggtt
     2581 caaggaagtt ggcttataca gtttgtcatt aaaccatgga gcattacact cttagatgaa
     2641 accgtccggt gcgagagcat gctgggtggg gtgagagggc aggggagcaa tctccctacc
     2701 tactcgattt ttcaaggctc tcgattcatt atccataggt ctaaattatc cgtaaaaaat
     2761 ttcgctgttt ttgagttctt tcactttctc catctcattg cttttctctt tctctaaatt
     2821 cagggccaga agaaatgctt ttctcttgtt atattataat gtagtccaaa tcattatatt
     2881 gaattttctc taaggatcgc catcggaatt tcttccttaa taacaaattc ggaatgaatt
     2941 caggtgaata atcattaatg ttttaaatcg aatacgggca agggaaaatt ctttatcgaa
     3001 aactgaggaa aataactttc tatttatcat gctcaaaaaa ccttgatgga ggcgttcatg
     3061 tttatcatgg taaaactgaa aggcaaagtt tatctttgca ttgtaattat aatgcttaaa
     3121 gatgaatgat tcacagatag aaacagatct ctttgataaa tttcgaaatc tcagggtact
     3181 tgtagttggt gatgtgatgg tagattccta tatatgggga agggtagagc gtttatcgcc
     3241 agaggctccg gttcccatag tagatgtttt ggataggagt aacaggttag gaggagcggc
     3301 taatgtagcc atcaatatta aatcgttggg agccgaacct atattatgtt cggtgattgg
     3361 taaggattcc ccatcgagga cttttgttcg actgatgaaa gaatccgggc tcgaccaaga
     3421 cggtattttg cagagcaaca ataggattac ctcgaccaaa tatcgaatat ttggaaataa
     3481 tacacagctt ttaagaatag atgaagaaac caactgccct attttaccga gcgaccaatc
     3541 tcgtttactt caaattattg aattccaggt tcagaccaat catcccgata ttataatttt
     3601 tcaggattac gataagggcg tactcactca ggggataatt cgggatataa tgcaacttgc
     3661 taccgaagcc aaaatcaaag tttgtgtcga tcccaaacga ataaatttct gggcttacga
     3721 tgatgtattg cttttcaaac ccaatctgaa ggaattgaaa gaaggcctga agatgaatca
     3781 tatctatcag accgatgaat tcattctgaa agatgccatc atagccctac aaaacaagat
     3841 aaatgccgaa atcgttctgg ttacgctttc cgatgagggg atggtgataa gatattcggt
     3901 tgaaagtgag ggatttcaat atcagagtat tccggcacat ttgagacaca ttgccgacgt
     3961 ttcgggtgca ggcgatactg taattagtgt ggcaagtctt tgtttggcag ctggattttc
     4021 accgcttctt tcggccgaaa ttgccaatgt tgccgggggt ctggtttgcg aagaggtggg
     4081 tgtagttcca attaatgcaa aacgcttgtc ggaagaaatc gaacgattga atctatattg
     4141 aaaactggat aacgaagcat ggaaaatcat aggagtactc aacctattct ttttgatgaa
     4201 attgcaccca gatatgactt tctcaatcat cttctttcgg taggaataga tatttcatgg
     4261 cgtagaaaac tggtcaaccg tttacctgaa aacaaccaac cttatatact cgatgtagcg
     4321 accggcacgg ccgatctggc tattatggct gcaaaaagaa taccctgccg aatcatcggt
     4381 tacgatattg ctgatagaat gcttgaaata ggggaaagaa aaattgcaca aaattccctc
     4441 acgcataaga tacaactcat tcgaggttct gttgaagacc ttccctttgc cgataattca
     4501 ttcgatgctg taatggtggc ttttggcgta agaaacttca aaaatctgcc gaaaggtctt
     4561 tctgaaatgt accgagtatt aaaacccgac ggaatttcac ttatccttga attttctatt
     4621 ccagaaaata aaatcgtaag gaatctgtat catttttatt tttttagaat atttccctta
     4681 ataggtggtt ggttgtcgaa aaaccgcaag gcatacactt atttgcccga atcggtacag
     4741 caatttcctc aaggtaagca ttttgagagg atgttgaaag gaatgggatt tcgtcgcacc
     4801 acatttcaat cgcttacctt cggcatagcc atactttatg aagctataaa ataaaacaaa
     4861 tcaaaaataa agctctatcg ctttcgttta tccatcaaat cgaatgatgc gccagatacg
     4921 caaaatacat tttctcggtt ttttattcct tctgatctcg gtaatggttt attctcagga
     4981 aggtccatcg gtgatgaatt taccaaagtt cgatcgcgca tctcgacatt ttggattcta
     5041 tgtatctggt aatcagatgt tttttacgat tcaaccggcc gataagtttc aacaaaaagt
     5101 ttggaatcgc gaccaaattc ccgaccttgt ggccgactcg gcccgggtta ttggtatcga
     5161 aagttctcct gtgccgggct tttccattgg tttggtttca aacttacgct taggtgaata
     5221 tttcgacctt cggttccttc cgggacttca gttcggagaa agagagctgc attattcggt
     5281 tatgcaattt tcacatgccg attcgtctat cgtcaatatt tcgaaaaaca tcaattctac
     5341 tttcatcgac tttccgcttt atgtcaaata taaatcgaag cgtttaaata atttccgagc
     5401 ctttgtgact gccggagcgt cttataaaat cgaccttgct tcttcaacaa aagacaaggc
     5461 cgaaaatccc tccaatgtcg tggtaaaatt gaaaaaacat gattttacgg gtgatttagg
     5521 agtaggtttc gatttctata ccgtttattt taaattttcg accgagatca aaatgtcgta
     5581 tgggattaac gatatgatat atagagaagg gaacatctat acggacagca ttaagaaatt
     5641 atctgcaaaa atgttccaaa tttcttttac ctttgaataa ctaatataca cactatggac
     5701 ggaatttcga acgaagaact tttttcgaca ttaaacgata agggcaatct taaaaaagaa
     5761 atctatcaga ataccgaacg ggcttttgaa ttgatcaatc aagctgcacg caagatttct
     5821 cagatttcac ctatgaagga tgagcatttg agaattgaat ttaaccggat aagtccattc
     5881 caaagccaat tatttttcgc tggtgatgcc ttgatatttt ttatgcaaac cgacatgtac
     5941 gagttttcgc gcgatcatga tgtaatgcga acggcttaca tccgccaaga caaaacccgg
     6001 agctacgtgg gcattattc
//
LOCUS       DAORPD010000006         5970 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_2418_length_5970_cov_2.177346, whole genome shotgun sequence.
ACCESSION   DAORPD010000006 DAORPD010000000
VERSION     DAORPD010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5970
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2418_length_5970_cov_2.177346"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            414..1001
                     /locus_tag="PK976_00175"
     CDS             414..1001
                     /locus_tag="PK976_00175"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC66335.1"
                     /translation="MRQVLVFTVYVLSFLWLSWMDGIDNLNAQSYYGFHPRMCLASYP
                     ELNHENYYPPGRDSKGEDIDANHEDAEWFAPEYFFQYSIDKNTGKLTVSCALEKVEVE
                     HNRERGDIDSQSEDSCRCSSSIVIIPNPANDEVILKGACNSSVYVFDFKGRQLLFSRI
                     EDIPRLDIRSLNTGIYSIKVECAEDIYTLQLIIQR"
     gene            complement(1005..2123)
                     /gene="hydE"
                     /locus_tag="PK976_00180"
     CDS             complement(1005..2123)
                     /gene="hydE"
                     /locus_tag="PK976_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HRC66336.1"
                     /translation="MNPKPSIRHLLGKKTFSHDELVILLSADKGEQNLIFQKASEVKQ
                     NNVGNKVYLRGLVEYSNICSKNCFYCGVRSGNKNVRRYKLSESEVLEAAQFVLDQHFA
                     SMVIQAGELSSPQNTCRIGELVRKIKQLSQGKIGITLSLGEQSREIYRYWHDCGAHRY
                     LLRIETSNPDLFQKMHPQDTFHTFEKRLKALEDLRNEGFQVGTGVMIGLPFQTYDDLA
                     FDLEFFKNLDIDMVGMGPYIEHKDTPLYQYKDLLLSPLERLDLSLKMVALLRIIMPDI
                     NIAATTAMETIDPNAKIRALKVGANVIMPNLTPLKYKEDYLLYEGKPNIDEEAQQTLN
                     YLIQSISAIHCEIGWDNWGDSLHFFHRHPENKEIEPHS"
     gene            2297..3814
                     /locus_tag="PK976_00185"
     CDS             2297..3814
                     /locus_tag="PK976_00185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HRC66337.1"
                     /translation="MKILSVDKIREADAYTIANEPIASIDLMERAARQCSQWLLDHLV
                     KDMPVMIFCGTGNNGGDGLAVARHLFENGFKVSVYILRFGSQASPDFLTNLCRLESLP
                     VECMDIASAGDFPEIPPFSYIIDALFGTGLSRKLEGLAVELVSYLNHSRGIIISIDIP
                     SGLFVDTSLKAVSGIAVQADHTLSFLPPKLCFMMPENDIYVGEWHVFDIGLHPEYLQQ
                     AETKYFYSSKDDVTSWLKRRRRFQHKGDFGHALLIAGSEGKAGAAIMAARACVHSGAG
                     LLTVHAPGKVGEALQINLNEAMVSFDEQASFITHLPSFDRYDAVGAGPGLGLHRQTQT
                     ALKLLIQNYRGPLIMDADALNILSENKTWYSFLPPYTILTPHLGEFERLAGKTDNDFE
                     RLKLAKDFALKYNIFLVLKGGVTIIVNPLGQCYFNSTGNPGMATGGSGDVLTGIILSL
                     LAQGYNHGQASVMGVFLHGFAGDLAAEELSQEYMSAVDIIRFLAPAFQSLSKGRG"
     gene            complement(3762..4454)
                     /locus_tag="PK976_00190"
     CDS             complement(3762..4454)
                     /locus_tag="PK976_00190"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HRC66338.1"
                     /translation="MSSPLAFIFDMDGTIVRNHENHLNAWKLFCQNHHIAITEEELLA
                     VFGGKNEDILQKVFVHKLSQKELRQFEEEKEGLYRGLYKPYIKEVEGLSDLLKNLFIA
                     QIPAALATSAPTSNVDMVIDALDIRKYFSCIIDATMVTHGKPHPEIFLKVAECLREKP
                     QNCLVFEDSRVGIEAAINAGCMVAVVKSPFTKERFTGANFLIDDFRDPRIQNLINPVL
                     SIKIEKQGPKTE"
     gene            complement(4456..>5970)
                     /locus_tag="PK976_00195"
     CDS             complement(4456..>5970)
                     /locus_tag="PK976_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66339.1"
                     /translation="NLTWARLSRMGNSLHRTTRKTVIRNNLLFKEGEKLDPYIMAENA
                     QLLRSLPYFSEAYLRPVADEKNPEKVDVILWAKDALSLGLDIYLPSSTLVDFSLFDQN
                     FLGYGNRLSSTVRYNTAKSPVFSLKNLNYRIENIKGTFFYSEADYTNEFNHESIRLTI
                     ARDFLTTQTPFASGLSLTHHEKIQNIFTPYFRQDRLKYFNPSLWIGKAFNVYGDGQPT
                     QLVISSSIDSRLYTQRPYVSADSNKIYYNSSNILLNIAISRNAYYKGNYIYQFGRTED
                     IPYGFLAELTTGPESYEYYNRTYFGFTTGVAKYFNRLGYLSGKFLISSYNRSGKFEQG
                     QWGTHLEYFSNLYIYGPFKLRHFLTLNYCQGMNRLEGESVNVVKELDLLIAQPSDTAF
                     YSGNEKLTLNYSSTLFTPYYFYGFRLAFFDFVNLAYLCGRQQFPEKNKFFSGFGIGAM
                     VRNENLVIKTILLKLGFYPGLPDNRSGVFIEFSGIAPLNFFNYKPHQPLISKYE"
BASE COUNT         1705 a         1059 c         1350 g         1856 t
ORIGIN      
        1 agaaactaac ctattgtcta tgaggaaatt aatcatcgag tatcatcaac gacacatcag
       61 gatggaaagg aacagcgctc caaatataag acatttttta acacaatgca agctttttag
      121 aaaatatttt aaaatattta tttattgtat ttattttcag cgtattaaat ttcgcgagta
      181 tcgagctttc ccttccttat ttttgttttg ggcaataaat ttttgatgtt taatagatta
      241 cgaaattaaa tttcgagatt tctgcaaacc gcattatata tgggttatca aatgatttga
      301 ggggtggcat gcttttatct taacctccga tggagtatga attcgagacg aaaggtgatc
      361 atagtgataa gaaaatgtgt atttttggag cttgagctga aaagatcgaa attatgcgac
      421 aagtattggt atttacagta tatgtgcttt cattcttatg gttaagctgg atggatggaa
      481 tagataattt gaatgcacaa tcatactatg gtttccatcc tcgaatgtgt ttggccagtt
      541 atcccgaact aaaccatgaa aattattatc caccaggtcg agattcgaag ggggaagata
      601 ttgatgcaaa ccatgaagat gcggagtggt ttgcaccgga atatttcttc cagtactcta
      661 tagacaagaa cacagggaaa ctaacggtaa gttgtgcttt agaaaaggta gaagttgaac
      721 acaatagaga gagaggggac attgatagcc aaagtgaaga ttcatgcagg tgttcatcat
      781 ccattgtgat aatacccaat cccgccaacg atgaagtgat tttgaaaggt gcctgtaatt
      841 catcggtgta tgttttcgat tttaagggga gacagttgtt gttcagccgt attgaagata
      901 tacctcgcct cgatatccga tcactcaata cagggatata tagtataaaa gttgagtgtg
      961 cagaagacat atatactttg cagctcatta ttcagagata gttttcaaga atggggttcg
     1021 atttccttat tttcgggatg tcgatgaaag aaatgcagtg agtcgcccca gttgtcccat
     1081 ccaatttcac agtgaatggc agagatactt tgaataaggt aattcaacgt ttgttgggct
     1141 tcttcatcta tattgggttt cccctcatat aagaggtagt cttctttata ttttaaaggt
     1201 gtaagattgg gcataattac gttagctcct actttcagcg ccctgatttt tgcattggga
     1261 tcaatggttt ccatggccgt agtggcggct atgttgatgt cgggcattat gatgcgcagc
     1321 agtgcaacca ttttgaggga cagatcgagt ctttcgagcg gtgacaaaag caaatcttta
     1381 tattggtaaa gaggagtgtc cttgtgttcg atatatggtc ccatgcctac catatcgatg
     1441 tcgaggtttt taaaaaattc gagatcgaaa gcgagatcat cataagtttg aaagggaagg
     1501 ccaatcataa cgcctgtgcc cacctgaaac ccctcatttc ttaagtcttc gagtgccttg
     1561 agtcgtttct cgaaggtgtg gaaggtgtct tggggatgca tcttttgaaa taaatcgggg
     1621 ttagaggttt cgattcgcag caaatagcga tgtgcaccgc aatcgtgcca atagcgatag
     1681 atttcgcgac tttgttcacc caatgaaagc gtgatcccaa tttttccctg cgacagttgt
     1741 ttaatttttc tgaccagttc cccaattcga cacgtgtttt ggggtgatga taattctcct
     1801 gcttgaataa ccatagaagc aaagtgctgg tcgagaacaa attgcgctgc ttcaagtact
     1861 tcactttcac tcaatttgta acgcctaaca tttttattgc ccgatcttac cccgcagtaa
     1921 aaacaattct tggagcagat gttggagtat tcgaccaaac ccctgagata cactttgtta
     1981 ccgacattgt tctgcttaac ttcagaggct ttctgaaaaa ttaaattttg ttcaccctta
     2041 tcggccgata ataagattac caattcatca tgagaaaaag tttttttccc caacaagtgt
     2101 cgaatgcttg gctttgggtt catgcatcat attttctgca aagatagttc acaattctca
     2161 ggtggttaga tttgcgcttt cgataatgat tctttagaat tgcaaaagaa aaattcgtcc
     2221 aaagaatgaa gtttcataat aagttgttat tgaaagacgc tgttatcttc aggaaaatta
     2281 caatggggga taagctatga aaatattgag tgtagataaa atcagggaag ccgatgcgta
     2341 tactatagcc aatgagccca ttgcaagtat tgatctgatg gagagggcgg cgcggcaatg
     2401 ttcgcaatgg ttgcttgatc atttggtaaa agatatgcct gtgatgattt tttgcggtac
     2461 tggaaataat ggaggtgatg gtttggctgt ggcacgtcat ctattcgaaa atggtttcaa
     2521 ggtttcggtt tatattctca ggtttggcag ccaggcttcg cctgattttt taaccaatct
     2581 ttgtcgtttg gagtcacttc ctgtggaatg tatggatatt gcttccgctg gcgattttcc
     2641 ggaaattcct ccgtttagtt acattatcga tgccttattt ggcacaggtt tgagcagaaa
     2701 gctggaaggt ttggctgttg aattggtatc ctacctcaat cattcgagag gtattattat
     2761 ttcgatagat ataccttcgg gattattcgt agatacttca ttgaaagctg tttcggggat
     2821 tgcagtgcaa gccgaccata ccctgagttt tttaccaccc aagttgtgct ttatgatgcc
     2881 agagaacgat atttatgttg gagaatggca tgtgtttgat attggccttc atcctgaata
     2941 tttacagcaa gcggagacga agtatttcta ttcttcgaag gacgatgtta cttcatggtt
     3001 gaaacggcgg cggagatttc agcataaagg agattttgga catgccttat tgattgcggg
     3061 gagtgaaggc aaagctgggg ctgcaattat ggctgcaagg gcttgcgtgc attccggagc
     3121 aggattgttg acggtgcatg ctcctggcaa ggttggcgag gctttgcaga taaacttgaa
     3181 tgaggccatg gtatcgttcg atgaacaagc atcgttcatt acgcatctac cttcgttcga
     3241 tcgttatgat gctgtaggtg ccgggccagg attgggtctc caccgacaaa cccaaactgc
     3301 actcaaattg ctcatacaga attatcgagg tccactgata atggatgccg atgctttgaa
     3361 tattttatcg gaaaacaaaa catggtactc atttttgcca ccttatacca tacttacccc
     3421 tcatctcggg gaattcgagc gactggctgg gaaaaccgat aacgatttcg aacgtttgaa
     3481 actggctaag gattttgcac tgaaatataa tatttttctt gtactgaaag gcggtgtaac
     3541 tattatagta aatcctttgg ggcaatgtta tttcaacagt acgggaaatc ccggtatggc
     3601 aacgggaggg agcggagatg ttttaacggg aataatttta agtttattgg ctcagggata
     3661 taatcatgga caagcatccg ttatgggagt ttttcttcat ggttttgcgg gtgatctggc
     3721 ggccgaagag ctttcgcagg aatacatgtc ggcggttgat attattcggt ttttggcccc
     3781 tgcttttcaa tctttatcga aaggacgggg ttgattagat tttggatgcg agggtcgcga
     3841 aaatcatcga tgagaaaatt cgctccggta aatctttctt ttgtaaatgg cgattttact
     3901 actgctacca tacaacctgc gtttattgcc gcttctatac ctactcgaga atcttcaaaa
     3961 acaaggcaat tctggggttt ttcccttaaa cattctgcga ctttcagaaa tatttcggga
     4021 tgaggtttac catgagtaac catagtggca tcgataatac aggaaaagta ttttcgaatg
     4081 tcaagtgcgt caatgaccat gtcgacattg gaagttggag ccgaagtagc cagagctgca
     4141 gggatttgag caatgaaaag attcttgagt aaatcactca aaccctctac ttcctttata
     4201 taaggcttat atagaccacg atataaccct tctttttctt cttcgaattg cctgagttct
     4261 ttttgagaaa gtttgtggac aaacactttc tgtaagatgt cttcattttt accaccaaat
     4321 accgctaaaa gttcttcttc agttattgct atatggtgat tctgacaaaa caacttccat
     4381 gcattcaaat gattttcatg atttctaacg atagtgccat ccatatcgaa gataaaggca
     4441 agagggctgc tcatattatt catatttgct gatgagaggt tgatgaggtt tgtaattgaa
     4501 gaaattcaag ggagctatac ccgaaaattc gataaaaaca ccacttcggt tatctggaag
     4561 tccaggataa aatccaagtt ttagcaggat ggttttgatg acgagatttt cgttgcgtac
     4621 cattgcccca attccaaaac ccgaaaagaa tttgtttttt tctggaaatt gttgtctacc
     4681 gcaaagatag gccaaattga caaaatcgaa gaaagccagt cgaaagccat aaaaatagta
     4741 tggagtgaag agagtagatg aataattgag ggttaatttt tcattgcccg aataaaaggc
     4801 agtatcgctt ggctgagcaa ttaacagatc aagttctttc accacgttga ctgattcgcc
     4861 ttcgagacgg ttcattccct ggcaataatt caaagtgaga aaatgtcgaa gtttgaaagg
     4921 gccgtaaatg tataggttag aaaagtattc aaggtgcgtt ccccattgac cctgttcaaa
     4981 ctttccgcta cgattgtatg acgaaatcaa gaatttaccc gaaaggtatc ccaaacggtt
     5041 gaaatatttt gccacgcctg tggtgaatcc aaagtaagtg cgattataat attcatagct
     5101 ttcggggccc gttgtgagtt cggcaaggaa accataggga atgtcttcgg tacgtccgaa
     5161 ctggtatata taattccctt tataataggc gtttctcgat atagctatat tgaggagtat
     5221 atttgaggaa ttatagtaga ttttattaga atcggctgac acataaggtc tttgcgtata
     5281 aagtcggcta tcaatactgg agcttattac cagttgggta ggctggccat cgccatatac
     5341 attgaatgct ttgccaatcc ataaagaggg attgaaatat ttcaaacgat cctgtcggaa
     5401 atagggggtg aagatatttt ggattttttc gtgatgagtg agtgataagc cactggcaaa
     5461 aggtgtttga gtggttagaa aatctcgagc aatggtcaga cgaatgcttt catgattaaa
     5521 ttcattggtg taatcggctt cagagtagaa gaaagttccc ttgatatttt cgattcgata
     5581 attgaggttt ttgagagaaa agaccggaga ctttgccgtg ttatagcgca ctgttgatga
     5641 aagacgattg ccatacccca ggaaattctg gtcgaaaagg ctgaaatcta caagagttga
     5701 gcttgggaga taaatatcta atcccaaaga taaagcatcc tttgcccata aaataacatc
     5761 tactttttcg gggttttttt catcggccac ggggcgtaaa tatgcttcgg agaaataggg
     5821 caaggaacgt aataactgag cattctcagc cattatataa ggatcgagct tttctccttc
     5881 tttaaacaat agattgtttc ttattacggt ttttcgcgtg gttctatgta aagaatttcc
     5941 catacgactc aaacgcgccc aggtaaggtt
//
LOCUS       DAORPD010000007         5860 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_2475_length_5860_cov_2.734539, whole genome shotgun sequence.
ACCESSION   DAORPD010000007 DAORPD010000000
VERSION     DAORPD010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5860
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2475_length_5860_cov_2.734539"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="PK976_00200"
     CDS             complement(<1..627)
                     /locus_tag="PK976_00200"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRC66340.1"
                     /translation="MKYKLPKNKWTDKQVEEHWDKVAHKYVKENNKVKYAHDQRFRKM
                     LELFPPLPSGAKILNITSRDAEASIFLQRTYPGIRVINAEISQGLMNEAFRLWPDIEQ
                     IKISTYSSLPFVDEEFDAIVSLETLEHVAEPVAFLKELHRVIRPSGILILSCPPATSE
                     FPYRIFTFLFGGHGEGPHRFLPSKKVKMLLKDTGWQVLHHEGTLLIPAG"
     gene            complement(631..1497)
                     /locus_tag="PK976_00205"
     CDS             complement(631..1497)
                     /locus_tag="PK976_00205"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRC66341.1"
                     /translation="MSNTPFISVIIPAYNEEEGLRNTLDKINSLHLFDRFEFIVVDDG
                     STDQTTFVAQQYPVRLLKHETNKGYGAALKTGIRKAKGEKVIFMDSDGQHDPAFLPKI
                     AQMLQDYDMVIGERTSDSYQVKARTSGKKIIRIVGEYLVEQKLPDFNSGFRGFDRQLI
                     LSLLHIMPNGFSFSTTSTLAFLKEGYSIGTFPIHVKERQGRNSTVKFLKDGPKTFLLL
                     FRMTMLFNPLKIFFPISVIAFIAGLGWGIHGYVVFSRFPNTATILILSSMFLFLIGLL
                     ADQISVMNRRKY"
     gene            complement(1663..3123)
                     /locus_tag="PK976_00210"
     CDS             complement(1663..3123)
                     /locus_tag="PK976_00210"
                     /inference="COORDINATES: protein motif:HMM:NF028437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5106 domain-containing protein"
                     /protein_id="HRC66342.1"
                     /translation="MRNVLFILAVSLFSVLSEVNAQGFQLKVKIKGANDDFLYLAHYF
                     ADKQYMDDTLRKGKGEWFVLEVDSMLTGGLYIIAGEKKNRYFEFLMNNEPRISFETIA
                     DDPVNNMQIKGSSENLAFFRYIHYLGKKQKEIEPYQKIFKRTEGLYPDSASLAKAWMQ
                     KINDEVMSYIKEFIESHEGMFAADFVKASQEPEIPDPPLLPNGRPDSLFAYKYYKAHY
                     FDNLPLNDARLLRTPFYHQRVNTYFTKMVLQHPDSIIAEAKRLIPIASQSADTYRYMI
                     WFLTNFTERSEIMGMDAAFVYMVENYYASGEMDYWINNTVKENLIKKAKKLKPILIGQ
                     TAPELIMLDTFMQPISLHQLKARYTILLFWDPTCGHCKKEIPMLKDFMTRKGKQYDIE
                     VFAVCSDTNMREMKNYIRQYDMDWINVNGPRAYTANYHDLYDIYSTPIIYLLDEKKTI
                     LAKRLTVEQIESFIEHHSRSESGGVLQMKQEYRNNK"
     gene            3245..4552
                     /locus_tag="PK976_00215"
     CDS             3245..4552
                     /locus_tag="PK976_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66343.1"
                     /translation="MTKKILILPFLFLGSFFYSQAQDSIINQLLRTRQKDYSNYIQQR
                     DSIKVRTWEKVVMLNGLAARVIQSDSILIAACLEQLAAGIPSVREQQLLDTITHLKGR
                     IEYMKQSSSPENDLSPRDYVILLGVSLILILLGIFALILLRKLQKANKSGLKTKEELE
                     TFQNTQLEYENKLEEINDELARIYNEREQLLQQITELNRALISEKQNNNQLKDCLETI
                     SNEKQEMQNEMKRLKEKAEKYQNLPDESTINEIITENNLLKSENIQLKQQILELQENI
                     EQLKSELLKVTESSDELLRKSLEEREQKYKMELQNAQQIQLQLEDKIKEIEERLKNIL
                     EEKDILVSEHNFVMESLSSMCDELERSKAQLQSVETEKISLEEQLVNLSPLPQQIDAL
                     NAEIIRWQNENEELKQQIAREIKLRKEIESDIQKIFNRLEGGV"
     gene            complement(4740..4874)
                     /locus_tag="PK976_00220"
     CDS             complement(4740..4874)
                     /locus_tag="PK976_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66344.1"
                     /translation="MDRDLLENILKLDNDEIELQLEEYGELEGRLPKKHKRYNIESFI
                     "
     gene            complement(5518..>5860)
                     /locus_tag="PK976_00225"
     CDS             complement(5518..>5860)
                     /locus_tag="PK976_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66345.1"
                     /translation="RIMFAEECYLERKYDQEYIEWTLKVPAFFPSFKNYVPSALPFSF
                     KSVLRREYSGMLSIVFGFAFIDYLRYYLLNRNFLSVRPSLYILVAALIIALVLRTIKH
                     HTKWLEESGRT"
BASE COUNT         1807 a         1107 c         1166 g         1780 t
ORIGIN      
        1 ccctgcagga atgagcaaag tgccttcgtg atggagaact tgccaacccg tatccttcaa
       61 cagcattttt acttttttgg atggcaaaaa acgatgtgga ccttcgccat gaccgccaaa
      121 aagaaaggta aaaatacgat aaggaaattc acttgtagct ggcggacagc tcaaaataag
      181 aatgcctgag ggacgaatga ctctatgcag ttctttcaga aaagctacgg gttcagccac
      241 gtgttcaagg gtttcgagac ttacgatagc gtcgaattct tcatcgacaa agggtaaact
      301 gctgtaagtc gaaattttta tctgttcgat atctggccac agtctgaaag cctcattcat
      361 caaaccttgc gatatctctg cattgatcac ccgaatgccc ggataagtac gttgcaagaa
      421 aatgctggct tctgcgtctc gactggtaat gttcaggatt tttgcccccg atggtaaggg
      481 agggaacaat tcgagcattt ttcgaaacct ctggtcgtgt gcatatttta ccttgttgtt
      541 ttcctttaca tacttatgtg ccactttatc ccaatgctct tcgacctgtt tatcggtcca
      601 cttgtttttt ggcagtttat acttcatgat ttaatatttc cttcggttca ttaccgagat
      661 ttgatcggcc agtaaaccaa tcagaaacag aaacatgctg ctaaggatga gaatagtggc
      721 tgtgtttgga aaccggctga aaacaacata gccatggata ccccatccaa gacctgctat
      781 aaaagctata acactaatag ggaaaaatat ttttaaagga ttaaacagca tggtcatgcg
      841 gaaaagtaat aaaaaggtct tggggccgtc ttttagaaat tttacagtac tgtttctgcc
      901 ttgacgttct tttacgtgga tggggaaagt gccaatagaa taaccctctt tgaggaaagc
      961 cagtgtagaa gtggtagaaa atgaaaaacc attgggcatg atatgtagta acgacaggat
     1021 gagctggcga tcgaaaccac ggaatccgga gttgaaatcg ggtaattttt gctcaactaa
     1081 atattcacct acaatacgta tgattttttt tccagatgta cgagctttaa cctgatagga
     1141 atcagaagtt cgttccccta taaccatgtc gtaatcctgc aacatttggg caattttggg
     1201 aaggaacgca ggatcgtgct gtccatcgct atccataaag attacttttt cgcctttggc
     1261 tttgcggatt cctgttttca aggctgcccc ataacctttg ttggtttcat gcttgagcag
     1321 acgtacagga tactgctgag ctacaaatgt agtttgatca gtagagccat catccaccac
     1381 aatgaactca aaccgatcga acagatgaag tgagtttatt ttgtcgagag tatttctgag
     1441 gccttcttcc tcattataag ccgggatgat aaccgaaatg aaaggagtat tgctcatgca
     1501 aatgaattcg ttttggcaaa gatactgtct ccatgtggaa tgagaaaata tgcttcagtt
     1561 tgcaggatgg tgaatggtga gttaaaaagt taaaagttga aggcaggtga tatgctggag
     1621 gtatggccta taacacgagt ttctgttttt atgtttacat gtttatttat tattcctgta
     1681 ttcctgcttc atttgaagca cgccaccgct ctcggaacgg ctgtgatgtt ctatgaatga
     1741 ctcgatttgt tcaacagtta gacgttttgc taaaatggtt tttttctcgt cgagcagata
     1801 tattatagga gtcgagtaaa tatcgtatag atcatgatag ttggctgtat aggctcttgg
     1861 cccatttaca ttaatccaat ccatatcgta ctgacggata taatttttca tttcgcgcat
     1921 attagtatca ctgcaaacgg cgaatacttc tatgtcgtat tgtttaccct ttcttgtcat
     1981 gaaatccttt agcatgggaa tttctttttt acaatggcca caagtggggt cccagaataa
     2041 aagtatggta tacctcgctt taagttgatg aagagaaatg ggttgcataa aggtatcgag
     2101 catgatgagt tcaggagcag tctggccaat taaaattggc ttaagttttt tggctttttt
     2161 tattaaattt tcttttacag tattattgat ccagtaatcc atttcacccg atgcataata
     2221 attttccacc atatacacaa aagccgcatc catgcccata atttcgctgc gttcggtaaa
     2281 attggtcaag aaccatatca tataacggta ggtgtcggct gattgactgg ctatggggat
     2341 taaacgcttc gcctcggcaa tgatgctatc agggtgttgt aataccatct tcgtaaaata
     2401 cgtattcact ctttgatgat agaagggggt tctcagtaat cttgcatcat tcagaggcag
     2461 gttatcgaaa taatgcgcct tgtaatattt ataagcaaac agagaatcgg gccttccgtt
     2521 aggaagtaaa ggaggatcag ggatttctgg ctcctgcgaa gctttcacga aatcagctgc
     2581 aaacattcct tcatgtgatt ctataaattc cttgatataa gacatcactt catcattgat
     2641 cttttgcatc caagctttgg ccaaactggc agaatcggga tacaaacctt cggtccgctt
     2701 gaaaatcttc tgataaggtt cgatctcttt ttgtttcttc cctaaataat gaatgtatcg
     2761 aaaaaatgca aggttctctg atgatccttt tatttgcatg ttgtttacag gatcatctgc
     2821 aatagtttcg aaacttatac gaggctcatt attcataagg aactcaaaat atcggttctt
     2881 tttttctccg gctataatat ataacccccc cgtaagcatt gaatcgactt ccagaacaaa
     2941 ccactctccc tttccttttc gaagcgtatc atccatgtat tgcttatcgg caaaataatg
     3001 agcaagatag aggaaatcat cattggcacc ctttatttta accttgagtt gaaaaccttg
     3061 ggcattcacc tcgctcaaca cggaaaatag agacactgca agaatgaata gtacatttct
     3121 catagactga gccttaatga ataaaattaa aatgtaattt taccttaaat acaccacaaa
     3181 aatattactt ttgtgaagaa gcattggtta aactcattaa aaatcccatt attccttcat
     3241 aaatatgaca aagaaaattc tcattcttcc ctttcttttt ctgggctcat ttttttatag
     3301 ccaggcacag gatagcatta tcaatcagtt gcttagaact cgccagaaag actattcgaa
     3361 ttacattcag caacgcgaca gcatcaaggt gcgaacatgg gaaaaagtgg tgatgctcaa
     3421 tggccttgct gccagagtga tccaatccga cagcatttta attgccgctt gtcttgagca
     3481 attggctgcg ggcattcctt cagttaggga acagcaactg ctcgatacca ttactcacct
     3541 caagggaaga attgaataca tgaaacagag ttcatcaccc gaaaacgacc tgagcccccg
     3601 tgattatgtc attcttttgg gtgtatccct catcctgatc ttgcttggaa tttttgcact
     3661 tatcttgctt agaaaacttc aaaaagccaa caaatccgga ctaaaaacca aagaagaact
     3721 cgaaactttc caaaatacac agctcgaata tgaaaataag ctcgaggaaa tcaacgacga
     3781 actggctcgg atttataatg aaagggaaca actccttcaa cagataaccg aactgaacag
     3841 ggctttaatt tctgaaaaac aaaataataa ccaattgaag gattgtttag aaacaatatc
     3901 gaacgagaag caagagatgc agaacgagat gaagcgattg aaggaaaagg ctgaaaagta
     3961 ccagaatctg cctgacgaat cgactataaa tgaaattatt accgaaaaca atctcctaaa
     4021 gtcggaaaat attcaattaa agcaacaaat cttggagctt caggagaata tcgagcaatt
     4081 aaaatcagaa ttactgaaag tcactgaaag ttccgatgaa cttttgcgaa aatctttgga
     4141 agaaagagaa cagaagtata agatggaact acagaacgcc cagcagattc aattacaatt
     4201 ggaagacaag atcaaggaaa ttgaagaaag gttgaaaaat attcttgaag aaaaggacat
     4261 tcttgtttcg gagcacaact ttgttatgga aagcctatct tcgatgtgcg atgaactcga
     4321 aagaagcaag gctcaattgc aaagtgttga aaccgaaaaa atcagtctcg aagaacagct
     4381 tgtgaatctt tcgcctttgc cccagcaaat cgatgctttg aatgctgaaa tcattcgttg
     4441 gcagaatgag aatgaggaac ttaaacagca aatagcccga gagataaagc tgcgtaaaga
     4501 gattgaatcg gatattcaga aaatcttcaa ccgactcgaa ggaggagttt gaactcacag
     4561 agtaaattcc attgctaaca ggttgctctt tcaaaggttt tttaagattt ttgctctgat
     4621 gcattcatag agtttcagtg ccttggggtc gtttccaacc agtttttgtt tctcgagttc
     4681 ttcgattatt ttctcgaaaa gagttaatcc gatttaatgt cgttgatgaa tcgttctttt
     4741 tatataaagc tttcaatatt gtaacgtttg tgtttcttgg gtaatctccc ttctaattct
     4801 ccatattctt ccagttgcaa ttcaatttca tcgttatcga gtttaagaat attttcgagc
     4861 aggtcgcggt ccatgaatat actttctttt aaaaacatcg ccattccctg cctttttgat
     4921 aaataaaatg gagaattctt tatcctataa ttgcatttca atccccttag ttatgacggg
     4981 acaaaagcag catcgcattt caaattgttg tattttcgtg taaacctaac aggttttaga
     5041 tatttgttag gttttatcat taaaataact tggcttaagt gagtctgata gatattacca
     5101 ggtcaggaaa ggaaacttaa aaaattgtgg gtttcggatg aagggagtgt gcctttacca
     5161 cgtgatgaaa gtaatcattg agaataggtg atgctatatc gcaatcttca aaaccacgac
     5221 aacttgattt agtgtcgaat caggagttct gaaattattc gggtatttca taacaaaaag
     5281 attttacaaa actgattcgg gaaaacattt ataacagaaa gcatttcatt ttttcttgtt
     5341 tccaatcctg ccaatggtaa ttagaaaacc aaagtttagg ggtaagatat ttgtcttttg
     5401 ccttttataa atatttacag tatttactct cgctcgagct tttgcaggca gtaggcatat
     5461 ttcgttcatt tttccacaac cctcctaaat ttatttttct atggtggaac tcatctttta
     5521 tgttcgacca gattcttcca gccattttgt gtggtgtttt atggttcgaa gaaccagcgc
     5581 aatgatgagg gcagcaacca ggatatacaa tgaaggtctg acagatagga aatttctatt
     5641 cagcagataa tagcgcaggt aatcgatgaa ggcaaaacca aacacaatgg atagcatacc
     5701 cgaatattct ctccgtaata ccgatttgaa agagaagggt aatgcagaag gaacataatt
     5761 tttgaaggag gggaaaaagg caggaacttt caatgtccat tcgatatatt cttggtcata
     5821 cttgcgttcg aggtaacatt cttcggcaaa cataattctt
//
LOCUS       DAORPD010000008         5841 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_2486_length_5841_cov_2.103180, whole genome shotgun sequence.
ACCESSION   DAORPD010000008 DAORPD010000000
VERSION     DAORPD010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5841
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2486_length_5841_cov_2.103180"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            169..2313
                     /gene="scpA"
                     /locus_tag="PK976_00230"
     CDS             169..2313
                     /gene="scpA"
                     /locus_tag="PK976_00230"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016277082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HRC66346.1"
                     /translation="MKPNFEDISAFSTEQFPEVVAPAEIPSWETPENIRVKSVYTAED
                     LANMEHLQYAAGIPPFLRGPYSTMYVMRPWTVRQYAGYSTAEESNAFYRRNLAAGQKG
                     LSIAFDLATHRGYDSDHPRVIGDVGKAGVAVDSILDMKTLFDQIPLDQMSVSMTMNGA
                     VLPIMAFYIVAGLEQGVKLEQLSGTIQNDILKEFMVRNTYIYPPQPSMRIIGDIFRYT
                     SKYMPKFNCISISGYHMQEAGATADLELAYTLADGLEYLRTGINAGLQIDEFAPRLSF
                     FWGIGMNHFMEIAKMRAARMLWAKIVKQFDPKNPKSMALRTHSQTSGWSLTAQDPYNN
                     VARTCIEAMAAALGHTQSLHTNALDEAIALPTDFSARIARNTQIYLQEETQICRTVDP
                     WAGSYYVESLTHHIAHRAWKLIQEIEDLGGMAKAIETGLPKLRIEEAAARKQARIDAN
                     KDIIVGVNKYRLEKEEPIEVLEVDNTAVREAQIRRLNELKASRDSEAVKKALDAITKA
                     CTTGEGNLLELAVEAAKARATLGEISYACEKVFGRYEAVIKSVSGVYSLESKDDDNFR
                     KACEMADEFARLEGRRPRIMIAKMGQDGHDRGAKVVATGYADIGFDVDIGPLFQTPEE
                     AARQAVENDVHILGVSSLAAGHKTLVPQVIEELKKLGRDDILVIVGGVIPPQDYEFLY
                     EAGVAAIFGPGTVVSEAAIKILEILIKSRKAE"
     gene            2463..4064
                     /locus_tag="PK976_00235"
     CDS             2463..4064
                     /locus_tag="PK976_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HRC66347.1"
                     /translation="MKKSPQIPHTYVIVFAIIFLAAMLTWIIPGGQFSRQTIRTSDGT
                     REVIDAHSFQSIDHQPQTWQIFSAFYKGFIRTPKIIVFILILGGAFWLLNESKAVDVG
                     VYSFLAKTQKFNRYKFFRKIGVNNLIIAVVMLIFSFFGAIFGMSEETIAFVIIFVPMA
                     ISMGYDSIVGVSMCFIGAGIGFAGCLMNPFTLGIAQEIAGLPLFSGIEYRFFIWIIVN
                     IFGIGFVLWYANHVKRNPRLSPVYENDQYWREQHANRQNLSLTPANKSTYLAYSLILS
                     AALVAAILLPINQIRLGNTSFKLPALPIAALLFAFFGWVSLKKSREFFMLNILGFTII
                     YLIIGVTGFSWGLLELATLFFSMAILIGIGLGKTPNQITQSFMAGAKDILSAALVVAL
                     AGGIIVILEDGKIIDTILYSISNSIQGAGKISSLSLMYLFQSAMNAIITSGTAKAALL
                     MPILRDVSDMIGLSQQATVTAFHIGDGFTNLITPTSGVLIGVLEVARIPFVKWFKWAW
                     KFILMMVILGWLLLIPTVTIPLNGF"
     gene            complement(4845..>5841)
                     /locus_tag="PK976_00240"
     CDS             complement(4845..>5841)
                     /locus_tag="PK976_00240"
                     /inference="COORDINATES: protein motif:HMM:NF019365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015926 - glucosidase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HRC66348.1"
                     /translation="PDSFHHIAPTEDQIRPAGINDKERIMGADISFLPQLEAEGMKFY
                     DFGNNIPQDVFEILKKYGFNYIRLRIFYQPENPEGYSPRKGYCNLAQTLEMARRIKKA
                     GMKFLLDFHYSDYWADPGKQYKPKAWDSLPFDILKDSVRNYTVRVLRALQAQNTLPDM
                     IQPGNEINHGLLWPEGHISQPDHLADLLQASISGIKEVAPDIPIMLHLALGGQNDESV
                     FWLNNMFARGIDCDVIGLSYYPRWHCTLDDLKYNMRDLIRRYGKDIIVVEYSQKKEEV
                     AGAVFGMPNDKGKGCFIWEPLNTWEGIFDKNGKPTRWLHAYENIKAMYLDRNLNK"
BASE COUNT         1738 a         1159 c         1245 g         1699 t
ORIGIN      
        1 attgtctctt aatgataaat tcctaatgac aaattatcat tcattaaaaa caatagaagt
       61 acataaaaac ttttgtataa gaattacaat aaaaaagcat tgaattactg gacaaggaaa
      121 gtaaatttct gcaaaactga ataaattaaa agattgaaat aaaatataat gaaaccaaat
      181 ttcgaagaca tttcggcatt ctctacagag cagttccctg aggtagttgc accggcggag
      241 attccttcat gggaaacgcc cgagaacatt cgggtaaaat cggtttatac agccgaagac
      301 ctagccaata tggaacactt gcaatacgca gctggtattc ctccctttct tcgcggccct
      361 tacagtacga tgtatgtcat gcgtccatgg acagtccggc agtatgcagg atattctacg
      421 gccgaagaat ccaatgcctt ttaccgtcgc aatctggccg cagggcagaa gggactttcc
      481 attgccttcg atttggctac tcatagaggc tacgactccg atcatccaag agttattgga
      541 gatgtcggga aagcgggagt tgctgtagat tccatactcg atatgaaaac cctgttcgat
      601 cagattccac tcgaccagat gtcagtttcg atgactatga acggagctgt acttcctatt
      661 atggcctttt atatcgtagc cggactggag caaggcgtaa aactggagca actttcgggc
      721 accatacaaa acgacattct taaggaattc atggtgcgta atacctatat ttatcctcct
      781 cagccttcca tgcgtatcat tggagatatt ttccgctata catcgaaata tatgcccaaa
      841 ttcaactgca tcagcatttc gggatatcac atgcaggaag ccggtgctac tgccgacctc
      901 gagctggctt atacgctagc cgatggcttg gaatatctgc gaactggaat caatgcaggc
      961 cttcaaatcg atgaatttgc gccccgcctt tctttttttt gggggatagg catgaatcat
     1021 tttatggaga ttgccaagat gcgtgctgct cgtatgttat gggccaaaat cgtgaaacag
     1081 ttcgatccaa aaaatcccaa atcgatggct ttacgtacac attcgcaaac atcgggctgg
     1141 agtttaacgg ctcaagatcc ttacaacaat gtagcacgta cctgtattga agccatggca
     1201 gcagcattgg gtcacacaca aagcctgcac acaaatgctc tggatgaagc cattgcactg
     1261 cccaccgact ttagtgcacg tattgcacgc aatacccaaa tctatctaca agaagaaact
     1321 cagatttgtc gtaccgtcga tccatgggca ggctcctatt atgtggaatc attgacacat
     1381 catattgcgc atagagcatg gaaactcatt caagagatag aagacctggg aggaatggca
     1441 aaagccatag aaaccggcct tcccaagttg aggatagaag aagctgctgc acgcaaacag
     1501 gcaagaatag acgccaacaa ggatatcatc gtaggagtga ataaatacag actcgaaaaa
     1561 gaagaaccta tcgaagttct tgaagtagat aatactgccg tgagggaagc ccaaattaga
     1621 cgcttgaacg aactgaaggc cagtcgcgac agcgaagccg tcaagaaggc attggatgca
     1681 attaccaaag cttgtaccac aggtgaaggc aatcttctgg aactagccgt tgaagctgct
     1741 aaagcaagag caaccctggg tgaaatttct tatgcatgtg aaaaagtatt tgggagatat
     1801 gaagccgtga ttaaaagtgt atcaggagtt tattcgttag aatcgaaaga tgatgacaat
     1861 tttcgcaagg cttgcgaaat ggccgatgaa tttgcacgcc tcgaaggtcg aaggcctcgc
     1921 atcatgattg ctaaaatggg acaggatggc catgatcgcg gagccaaagt agtagccaca
     1981 ggctatgccg atattggctt cgacgtggat atagggcctt tgtttcaaac ccccgaagaa
     2041 gccgcccgac aggccgtcga gaatgatgta catatactcg gagtttcttc tctggccgca
     2101 ggtcataaaa ccctcgtccc tcaggtcatc gaagaactca aaaaactcgg acgcgacgac
     2161 attttggtga ttgtaggcgg agttattcca cctcaggatt atgaattttt atacgaggcc
     2221 ggtgtagcag ccatatttgg cccaggtaca gttgtgagcg aagctgctat caaaattctc
     2281 gaaatcctta tcaaaagccg aaaggctgag taaactttat tataagataa agacaatagc
     2341 attcgttccg ttgcattccg atgatataaa tgcgggcaaa gactggcttc tgaaattatc
     2401 atctatacga attcgataaa ttcttctaag tttgagggta gattttagct gcctcatctt
     2461 ctatgaaaaa aagtccacaa atccctcata cttatgtaat tgtttttgcc atcatctttt
     2521 tagcggctat gcttacatgg ataatccctg gggggcaatt ttcacgccag accattcgaa
     2581 caagcgatgg cactcgtgaa gtaatagatg cacattcctt ccagtcgatc gaccatcaac
     2641 ctcagacatg gcaaattttt tcggcatttt ataagggatt tatcagaaca cccaaaatca
     2701 tcgtgtttat tttgattctt ggaggagctt tctggctgct caacgaaagc aaagctgtag
     2761 atgtaggagt ttattcattt ttagcaaaga cccagaaatt caatcgatat aaattctttc
     2821 ggaaaatagg ggtcaataac ctgatcatcg cggtcgtcat gctgattttc agtttttttg
     2881 gagcgatttt cggcatgagt gaagaaacca ttgcctttgt gattattttt gtgcccatgg
     2941 ccatctctat gggctacgat tctatagtag gagtgagtat gtgttttata ggtgcaggaa
     3001 ttggctttgc tggttgcctg atgaatccct ttacgcttgg aattgcacag gaaattgcgg
     3061 gcttgccttt attttcggga atagaatacc gtttctttat ctggattata gtaaatattt
     3121 ttgggatagg ttttgttctg tggtatgcta accacgttaa acgaaatcca cgcttaagcc
     3181 cggtttatga aaacgaccag tactggaggg aacaacatgc caatcggcaa aatttaagtc
     3241 ttacgcctgc caataaatcc acttaccttg cttattctct tattctgagt gctgctcttg
     3301 tggcggctat tcttcttccc ataaatcaaa tcagattggg aaatacaagt ttcaaattac
     3361 ccgctttgcc aattgcggct ctgttatttg cattttttgg ttgggtgagt ttgaaaaaat
     3421 ccagagaatt tttcatgttg aatattcttg gttttaccat aatctattta attattggtg
     3481 taacagggtt ttcgtggggt ttgctcgagt tggctacctt gtttttttca atggctattc
     3541 tcataggtat aggtctgggc aaaacgccca atcaaattac ccaatccttc atggcgggag
     3601 ccaaggacat tctgtcggct gctttggtag ttgctttggc tgggggtatc attgtgattc
     3661 tggaagatgg aaagattatt gacaccattt tatatagtat atccaattct atccagggag
     3721 ccggaaaaat tagcagcctc tccttgatgt atctatttca gtcggccatg aatgcaatca
     3781 taacgagtgg aacggcaaaa gctgccttgt tgatgccaat attgcgtgat gtatcagaca
     3841 tgataggttt atcgcagcag gcaaccgtaa ccgctttcca tatcggcgat ggatttacca
     3901 atctcattac tccaacttcg ggagtattaa tcggagtgct tgaagtagcc cgtatccctt
     3961 ttgtcaaatg gttcaaatgg gcttggaaat ttattctaat gatggttatt ttaggctggc
     4021 tgttgcttat ccctacagta accatcccat tgaatggatt ttaatgtagc agaatttata
     4081 ttatatgaac ttctattaga gaatatcaat gatcggctaa aactactgta ttagccccta
     4141 taaattagga gaatagtata aatataatct atacttttat gcctataaaa aaaagaaaat
     4201 ttaccagaga ggaaaatctt caaattatta agtaaacctc ccagattagt gttaaacaaa
     4261 tccttaaaaa ataaggagta tatcaagtat cctactattc atagaagaag aagtttgaag
     4321 agatgggaga agaaggtttt cacatgaaat gactccaatg catttaaaac gtattcgtga
     4381 attggagaaa gaagtaactg ttttaaagca acttttagcg gtgaaggaac tggaaagcat
     4441 gttaagaaag gtgttttaaa aaaagttagt cttggagaga agaagggaat ggcagctcga
     4501 tacaatcatc ttggtttaaa gcttgaaacc acattggttt tggctgaaat taagcgacat
     4561 caaaattact atcattcctt ggggaaggaa aagaggggaa agaccgagtg aaaccacact
     4621 tattgtaata aaaaaacaga gtagaacgta tacgctttaa gaatgagaac ataggaaaaa
     4681 atgtcaagcg ccgaaacatt ccctcaacca ttatcggtat aacaataatc gttggttgtt
     4741 gcttgtcaac gcaaatttct ctagattttt taaattttgt aaaattatct atgaaatttt
     4801 tcgaataaca agggtctaag cctccacatt gatttttata aatctcattt atttaaattt
     4861 cggtcgagat acatggcctt gatattttcg taggcatgca accatcgagt aggtttgccg
     4921 tttttatcga atatgccttc ccaagtattc aagggctccc agataaaaca accctttcct
     4981 ttatcgttag gcattccaaa tactgccccg gccacttctt cctttttttg agaatattct
     5041 accacaatga tgtctttgcc atatcgcctt atcaagtctc tcatgttata tttcaaatca
     5101 tcgagggtgc aatgccagcg tggataatac gaaagtccaa ttacgtcgca atcaatgcct
     5161 ctggcaaaca tattgttgag ccagaagacc gattcgtcgt tttggcctcc caacgcaaga
     5221 tgtagcataa ttgggatgtc aggtgcaact tccttgattc ccgaaataga agcctggagt
     5281 aagtcagcca aatgatccgg ttgagaaata tgaccttcgg gccatagcaa accgtgatta
     5341 atttcattac ccggctgaat catatcgggt aacgtatttt gagcctgcag ggcacgaagc
     5401 accctaacgg tataatttct tacggaatct ttcaaaatat cgaatggtaa tgagtcccag
     5461 gctttgggtt tatattgttt gcctgggtca gcccaataat cgctataatg aaaatcgaga
     5521 aggaatttca ttcccgcttt tttaattcgt cgggccattt cgagggtttg tgccaagttg
     5581 caatatcctt tcctggggga ataaccttcg gggttttcgg gttgataaaa gattcttaat
     5641 cgaatataat taaatccata tttttttagg atttcgaata catcttgagg tatgttgttg
     5701 ccaaaatcat aaaatttcat tccttcggct tccagttgcg gtaagaaact gatatctgca
     5761 cccataattc tttccttgtc atttatgcct gcaggtctga tctgatcttc ggtaggagca
     5821 atatgatgaa aggaatcagg a
//
LOCUS       DAORPD010000009         5649 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_2578_length_5649_cov_2.522882, whole genome shotgun sequence.
ACCESSION   DAORPD010000009 DAORPD010000000
VERSION     DAORPD010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5649
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2578_length_5649_cov_2.522882"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..3215)
                     /locus_tag="PK976_00245"
     CDS             complement(117..3215)
                     /locus_tag="PK976_00245"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRC66349.1"
                     /translation="MRITDTSVKNPVAIYMIFAGILLFGLLAIFRLPRDLFPDIELPT
                     LTVVTVYPGANTEQVEKEITKELEVVLASTENIKKIQSFSKDNVSTISLQFDWGTDIT
                     EASANARDLIELVKYKLPRDAQQPIIMKINSSVMPVVILGLQTTNEETDISDLIEKVI
                     SPRLQHIDGVGTVMTIAEPENEIAVEIDPLKAEKYHLTIDYIATLMKSLNLNVPAGSL
                     KSGIWDLNVTVKGQVPSIDAIKDLPLTTYMGQVIRLADIAIVHKQFKQKDEIARTHQQ
                     RATALLVQKQSQANALQVYQQVMKEVSKIPMPKETSLYEVFNTTEVIQGSINNITQTL
                     LWGGLWVILVVWLFLRAWRSSFIISLSIPFSMFIALITIIVSGYTINVFTLMSMVVAI
                     GMVVDNSIVVVENITKYLEKGIKKKEASIFATGEMGRAITASTLTTIAVFFPLIFAGG
                     IVGVFFEQFAIIGAATMLGSLLIALTLTPTLSSQFLLEESKQKKHPKIYDTTEKWYVK
                     LEEAYGRLIKWSLHNKILVTIIVAAFIALSVFLINNHIGTDYIPNIDGGDLITVVQMP
                     EGYSANETEKVAMRIEKYIESNVSGIVFGFTVAGQTEQGILSSVGFKENKNLATIVYH
                     LVPSNQRKMSTAEVAKMVEKELEKMPEIEQYHVTGGSIIGSALLGTTKPIEIVISGTD
                     LEILRKYAKMVEDSLMQLPDLKNVQNSLANTKPEFQITLDKERMADLKINPALASMQI
                     RQSLYGTEAGNFDDQGKQIPINVRYLRNEVQSINDIMNIRISSVLGNSYPLREVAKVN
                     LNNGYVEIMHDNQQRVAIVGADINSGTDLGVMVKRVQNVLSHLDTDNQVRIELKGQAE
                     EQAKSFADLRLILIISVLLVYMIMAAQFGHLLQPFIIFLSSPFAVVGSIIAFYLTGTT
                     LNLISFVAIILLMGVVVNNGIVLVDYTNMLVKRGYSLYDALIAAGRSRLRPVLMTTLT
                     TIMGMLPMAINRGLLHEVWAPFGITSIGGLMFATLITLIWIPVLYAIFYAHIQPKKLL
                     NV"
     gene            complement(3227..4285)
                     /locus_tag="PK976_00250"
     CDS             complement(3227..4285)
                     /locus_tag="PK976_00250"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRC66350.1"
                     /translation="MKKSIVSLGFIGIILLGGCNDKNRQNPSNHGGEKPKVEIYRIHT
                     QDVPLNLSYLGSFKPFKEATLGTTIPGKIEKIYTQEGEAVKEGQLLVQMSSELLLQSE
                     IEYKTLEKDFQRVKNLVEKQSISQQDFDHVKARYEAAEAKYNLLRSNTEIRAPFDGIV
                     AKHLAHEGENYFFSASIDLGTSLSTGILQLVKINPIYFEFEVGEKDLSYISKNMVVKA
                     IANTYSHDTIAGWVDFISPTLNSTRATANIRIKINNPNLKFRPGMTGKAFINVISHQV
                     MMIPLQAMMKELNHDAYFVYAVDNEGKIIKKPIKVFQYAGENVVVEGVSEGDEVVISS
                     KKNLAEGIEVEIIPTSND"
     gene            complement(4299..>5649)
                     /locus_tag="PK976_00255"
     CDS             complement(4299..>5649)
                     /locus_tag="PK976_00255"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRC66351.1"
                     /translation="IDSSNLQSAKYSSKSAFANYFPDISFSGTYLRLNKQFQMFKNDL
                     LLPVIPYQAIEAANMQVSISSLNNPQIAPLAFALDPATGMPFTDANGHPVFKNYAWLP
                     ASQSKLGEKDNFLFNLSLTQPIFVGGKIYFANKMAQNAVEIAICKTEIDRQSLLYEVE
                     DLYYKVWITEKKYRLALKYLDLLKEIENDVENYLKEGLITSNQLLMIRSKINQAQVDI
                     FKAENGLSVQKQLLNQKIGRDIKQDFKVEDSLKADFEIIQNILNTQLSIDNRPEVKLL
                     EQKVQLNRNLMRVTQADMMPQLGLTANYFYINPNPYKGLEDEFGDDYALGLSLKIPLF
                     HAFERYNKRQSALLQYKSAQLEMEDSRNLLNIEATYVTNQLKEQLMQIEAARKNVELA
                     RENLRIQTDMFHEGMLKLSDLTEAQTMWLKAETDYYEAYASLLSYHAKLRKSYGILQ"
BASE COUNT         1576 a         1143 c         1104 g         1826 t
ORIGIN      
        1 tatgcgattt tttactttaa cattgtctaa aatttgaaat gcgctaataa ggcattcttc
       61 gagtatttcg atgatttcat ttgcttgggc cacgtcgcaa tgaatattca catgtttcat
      121 acgttcaaga gttttttggg ttgaatgtgc gcataaaata tagcatacaa tacaggaatc
      181 cagataagtg taataagtgt agcaaacatc aggccgccaa tgctcgttat gccgaatgga
      241 gcccaaactt cgtgcaatag gcctctattt atggccatgg gcagcattcc cataattgta
      301 gtaagggtag tcatcaatac gggtcgcagt cgtgatcttc cggctgcaat caaagcgtcg
      361 tagagggaat aaccacgctt gactagcatg ttagtataat cgaccaatac aatgccattg
      421 ttcaccacaa ctcccatcag caaaataata gctacgaacg atatcagatt cagggttgtg
      481 cctgtgaggt agaatgcaat gatcgagccc accacggcaa agggagaaga caggaatatg
      541 atgaaaggtt gaagcaggtg gccaaattgc gcagccatga tcatgtaaac caataatacg
      601 ctgattatca atattaatct aagatcggcg aaactctttg cctgttcttc ggcttgtcct
      661 ttgagttcaa ttcttacctg attatcagta tctaaatgag atagaacatt ttgaactcgt
      721 ttgaccataa ctccaaggtc ggtgcccgaa tttatatcgg ctccaacgat ggctacgcgt
      781 tgttgattgt cgtgcatgat ttcgacatat ccattattga ggttcacctt ggctacttcg
      841 cgtaaaggat aagaattgcc taaaaccgaa gaaattctga tattcataat atcgttgata
      901 gattgaacct cattgcgtaa atatctgaca ttgattggaa tttgtttgcc ttgatcgtcg
      961 aaatttcctg cttcggtgcc ataaaggctt tgacgaatct gcatggaggc taaagcggga
     1021 ttgatcttca agtcggccat gcgttcctta tccagagtta tctgaaattc tggcttggta
     1081 ttggccaaac tgttctgaac atttttgagg tcaggtaatt gcattagtga atcctccacc
     1141 atttttgcat atttgcgtaa aatttcaagg tcagttcctg aaatgacaat ctcgatgggt
     1201 ttggttgtcc caagtaatgc tgaacctatg atagaacctc cggttacatg gtattgctct
     1261 atttcgggca ttttttctaa ttctttttcg accattttgg ctacttcggc ggtgctcatc
     1321 ttgcgttgat tggaaggaac gaggtgatag acgatggtag caagattttt attttcctta
     1381 aatccaacgg atgagagaat accctgttcg gtctgtcctg ctacagtaaa gccaaacaca
     1441 attcccgaaa cattcgattc gatatatttt tcgatcctca ttgcgacttt ttcggtttca
     1501 ttagcgctat aaccttcggg catctgaact acagttatga gatcacctcc atcgatgttg
     1561 ggaatataat cggtgcctat gtgattattg attaaaaata ccgaaagggc gataaaagcc
     1621 gcaacaatga tagttacaag aattttgtta tgaaggctcc attttatcaa tcttccataa
     1681 gcttcctcga gtttaacata ccatttttcc gtagtatcat aaatttttgg atgttttttt
     1741 tgtttggatt cttccaatag gaattggctg ctcagtgtag gtgtcaatgt cagagcgatg
     1801 agcaatgatc caagcattgt agcagctcct ataatggcga attgttcgaa gaatacaccc
     1861 acaataccgc ctgcgaaaat taaagggaaa aacaccgcta tggtagttag tgtagaagct
     1921 gtaatagctc gacccatttc gcccgtagca aatattgaag cctccttttt tttgatgcct
     1981 ttttctaggt atttagtaat gttttcgacc actactatac tattatcgac caccatccca
     2041 atagccacca ccatcgacat caaggtaaac acattaatcg tgtagccgct cacaataatg
     2101 gtaatgagag cgataaacat agaaaaaggt attgagaggc tgatgatgaa gctcgaacgc
     2161 caggctcgta agaagagcca tactaccaga attacccata atcctcccca aagcaatgtt
     2221 tgagtgatat tatttattga tccttgaata acttccgtag tattaaaaac ctcatacaga
     2281 ctagtctctt tgggcatagg gattttgctt acttccttca ttacctgctg ataaacctga
     2341 agcgcattag cctgagattg tttttggaca agaagagcgg ttgccctttg ctgatgagtt
     2401 cgggctattt catctttctg tttgaactgt ttatgtacga tcgcaatatc agccaatcgg
     2461 attacttgcc ccatgtaagt ggtaagcggc aaatccttta tcgcatcgat ggaaggcacc
     2521 tgacccttta cagtaacatt taagtcccaa atacccgatt tcaaagaacc cgccggaaca
     2581 ttcaaattga gacttttcat taaggtggca atataatcga tggttaaatg atatttctcg
     2641 gcttttaaag gatcgatttc tacggctatt tcattttcag gttcagcaat ggtcatcact
     2701 gtgcctactc catctatatg ctgaagcctc ggagaaatta ccttttcgat caggtcgctg
     2761 atatctgtct cctcattggt ggtctgaaga cctagtatca ccactggcat taccgaggaa
     2821 ttgatcttca tgatgatagg ttgttgagca tcacgaggca gtttatactt caccaattct
     2881 atcaagtcgc gtgcattggc cgaagcttct gtaatatcag ttccccagtc gaactgtaag
     2941 gaaatggtgg aaacattatc tttcgaaaaa ctctgaattt ttttgatgtt ttcggtactc
     3001 gccaaaacta cttctaactc cttggttatc tctttttcta cctgttcggt attggcaccc
     3061 ggataaacgg ttacaaccgt aagcgtagga agctcaatgt cggggaacaa atctcttggt
     3121 aaacgaaaaa tagccaataa tccaaataga aggattcccg caaaaatcat atagattgca
     3181 accggattct tgactgaagt gtcggtgatt ctcatagcct tagattttaa tcattggatg
     3241 tgggaatgat ttctacttca atgccctcag ccaaattttt cttgctggaa attacaactt
     3301 catcgccctc gcttactcct tccaccacaa cattttcacc tgcgtattga aaaactttga
     3361 tgggtttctt gatgattttc ccttcgttat cgactgcata tacaaaatag gcgtcatggt
     3421 taagttcttt catcatggcc tgcaagggta tcatcatcac ctgatggctg attacattga
     3481 taaaagcttt tcctgtcatt ccagggcgaa atttgaggtt tggattattt atttttattc
     3541 gaatattagc cgtggcccgg gttgaattca aagtaggact gatgaaatcg acccagcccg
     3601 caatggtatc atgagaataa gtattagcta tggcctttac aaccatattc ttactgatat
     3661 aggagagatc tttttcgccc acctcgaatt cgaagtaaat gggattgatt tttaccaatt
     3721 gcaatattcc tgtcgataag gaagtaccta aatctataga tgccgaaaag aagtaatttt
     3781 cgccttcgtg ggctaagtgc tttgccacta tgccatcgaa aggagctcta atttcggtgt
     3841 tcgaccgcaa taaattatac tttgcttcgg cagcttcgta acgagccttt acatgatcaa
     3901 aatcctgctg actgatggat tgcttctcaa cgaggttttt tactctctga aaatctttct
     3961 cgagagtttt atattctatt tccgactgaa gcaggagttc agacgacatt tgcaccagca
     4021 attgcccctc ttttacggct tcaccttctt gagtatagat cttttcgatt tttccaggga
     4081 tagtagtacc caaagtagcc tccttaaaag gtttaaaact ccccaagtaa gaaagattca
     4141 atggaacatc ttgggtatga atcctgtaaa tttctacctt tggcttttca cccccatgat
     4201 ttgaaggatt ttgtcgattt ttatcgttac agcctcccaa gaggattatt cctataaatc
     4261 cgagagatac tatgcttttt ttcatatttc ttctgttttt attgaagtat tccataagat
     4321 ttacgtaatt tggcatgata ggagagtaaa ctggcataag cctcataata atcggtttct
     4381 gcctttaacc acattgtctg tgcttcagtt aaatctgata attttagcat gccttcgtga
     4441 aacatatcgg tttgaattct caaattttct cttgccagtt ctacattctt tctggcagct
     4501 tctatctgca tcaattgttc cttgagctga ttggtaacat aagtggcctc aatgttcaat
     4561 aaattgcggc tgtcttccat ttccaactga gcagatttat attgtaacaa tgccgactgt
     4621 cgcttattgt aacgttcaaa agcatgaaat aaagggattt tgagcgataa ccccaaggca
     4681 taatcatctc caaattcatc ctcgagtcct ttatagggat tgggattgat atagaaataa
     4741 ttggcagtca aaccaagttg aggcatcata tcagcctgtg taaccctcat gagatttctg
     4801 tttaattgta ctttttgttc gagcagtttt acctcaggcc ggttgtcaat actgagttga
     4861 gtattcaaaa tattttgaat tatttcaaaa tctgccttca aagaatcttc gaccttaaaa
     4921 tcctgtttga tatctctgcc tattttttga ttcaacagtt gcttttggac cgataacccg
     4981 ttttctgcct taaatatatc tacctgggct tgattaatct tcgatcgtat catgagtagc
     5041 tgattgctag taatcaagcc ttcctttaaa taattttcca catcgttttc aatttctttg
     5101 agtaaatcga gatacttaag tgccaatctg tatttttttt cggtaatcca aaccttatag
     5161 tacaaatctt ctacttcgta caataaggat tgccggtcga tttcggtttt gcatatggct
     5221 atttcaacgg cattttgagc cattttattc gcaaaataaa tcttacctcc cacaaaaatg
     5281 ggctgagtca atgagaggtt gaacaaaaaa ttatctttct cgcccaattt tgattggctg
     5341 gctggaagcc atgcataatt tttgaaaaca ggatggccat ttgcatcagt aaatggcatg
     5401 cccgtggcag gatccaaggc aaaagcaaga ggtgctattt gaggattatt cagactggat
     5461 atcgaaacct gcatattagc tgcctctatg gcttggtaag gaattacggg aagaaggaga
     5521 tcatttttga acatttgaaa ctgtttgttc aatctcaaat atgttcccga gaaactaatg
     5581 tcggggaaat aatttgcaaa ggccgactta ctgctgtatt tagccgattg aagattgctg
     5641 ctgtctatt
//
LOCUS       DAORPD010000010         5505 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_2675_length_5505_cov_2.473211, whole genome shotgun sequence.
ACCESSION   DAORPD010000010 DAORPD010000000
VERSION     DAORPD010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5505
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2675_length_5505_cov_2.473211"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            49..1422
                     /locus_tag="PK976_00260"
     CDS             49..1422
                     /locus_tag="PK976_00260"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRC66352.1"
                     /translation="MKIGKIIAILLLFSISESLEAQNKVSMKIDSLLDEYFAVSNSHR
                     ELALSLAENARKLAQKHGEKHNEAVALECLGVQYYYRGDYNKARDFLNQSLEIYNEEN
                     SSTGLASVFTNLALVEQDLRNLNQAHYYYTTSLVYDRNSHDSIGMAFTINNLGTLYLH
                     KGDFQRARAYFQKAYDIGKSVHHLEVIGNSIGNFGLLRLESSNPDSAIPYFRKALSIA
                     KQYGDEYAQAIANINLGLCMIEKSEADSAERYLTIAARTSEELEDNELQIECLLAKAK
                     LMLLLNQPELANRILFSAQKLNNPTERKKTSAQIFTLIGKNLLLMNQPEKARNYFLQS
                     LDIAKEIGALPEMIDAYKHLAMFYSAISMYDSCELYIDLYAKEWSKLTIDTTLPDTSF
                     VYEAKSNNKNILHRISKNSQLSPLKLLVTLVFALTILWAGMIYLPKILLSLRKKTKKD
                     NSNTSAK"
     gene            1474..1881
                     /locus_tag="PK976_00265"
     CDS             1474..1881
                     /locus_tag="PK976_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66353.1"
                     /translation="MKLKHSGFILFLATILIFSITSCEDETVQRPVNQYQVKYEIKNH
                     LKKKCNFYIRYFNPKFDNIVAEYYDSINEWSRELKGKTFDHLYFEAFTVRDSAMFEIS
                     IYVNGQQVVRDVDSCPGPSVCDTNRIKVSYVLQ"
     gene            1924..3378
                     /locus_tag="PK976_00270"
     CDS             1924..3378
                     /locus_tag="PK976_00270"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC66354.1"
                     /translation="MGKGKIFRQFPSTFWAANVMELFERWAWYGIYMVLALYLTGSTD
                     TGALGFSQEQKGSLMGTVTFLLYLLPIITGAIADRYGYKKVLTIAFIILSTGYFSLAH
                     VRSYWSFYVLFLYLSVGAALFKPIITATVSHTTNDQTSSIGFGIFYMIVNIGAFLGPI
                     FASKLRILDWKFVFYLSSAIICINFILLFFFYKEPINKKQHTPILVSIKNIIKNTVTV
                     LADFKLMLFLIIMVGFWAMYLQLFYTLPIFIDQWMDTKILYDAIENFWPALAHIAGTS
                     QRTIAPEILTNIDAFYIVIFQLLVSSLVSRFSPLRAMTNGIMMATIGIALMFAFNNPI
                     YLFFSILIFSLGEMATSPKIQEYVGKIAPPDKTALYMGYSYLPLAGGHIVAGFISGSI
                     FAKIADKSTLLRNDLLSRGIVMPDISPTYTLNHFYSEASSLLHLDSSCLTSYLWQTYH
                     PGRIWILFSSIGLVSFLALLMYDRFFIRQKKILQ"
     gene            3378..4106
                     /locus_tag="PK976_00275"
     CDS             3378..4106
                     /locus_tag="PK976_00275"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRC66355.1"
                     /translation="MKKWFPSIFPALFITILMTAFFIFSNSSKISLIHLGIYPLSAKG
                     LLGIVLAPFIHENANHLYSNIIPFFVLTILLFYFYKDIAYEILGFSWIMTGILVWLGA
                     RPAYHIGASGVVYALMAFHVLSGMVRKNPRLLAISLLIVFFYGGMIWGIFPDFIPGRA
                     ISWESHLAGTITGSILAIYYRKEGPQRPLYSWELEPDEDDEDNEETKQNTPSQNLDKE
                     AEKAEEIMIKYIYKRKNTDEESSG"
     gene            4180..5283
                     /locus_tag="PK976_00280"
     CDS             4180..5283
                     /locus_tag="PK976_00280"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRC66356.1"
                     /translation="MKKNSLLSFPVLLAIMLFSCQKSDQYFLECNLEGLNDGMAYLQV
                     IGEDGIRTIDSTTISNGKFTFKGSQSEPIFAYITLNGKPDRIGLFLENARVSIYGHAD
                     SLEHIKISGSPLNDEYSKFKSMLASYENRQEELYLQYNQARKTNDTLALKNIETLWDL
                     LDKQQNKVIMQFISQHKHSVISPFLIHQRLVYNLNVNKLDSLTSIFPPELENSVYVRM
                     LKKRIQTLRNVEIGKPAPEIALDDTMGILRTLSSFRGKYVLIDFWASWCSPCREESPN
                     LVKAYQRFHDKGFEIFGVSLDTRRDKWVEAIRKDQLTWTHVSDLKGWKSEAAELYGVN
                     SIPHSVLVDPRGIIIQNNLRGKALIDFLEKLIK"
BASE COUNT         1710 a         1109 c         1029 g         1657 t
ORIGIN      
        1 gaatatatcc ctgttcaata agaaataaat tttgccaata aaaaataaat gaagattgga
       61 aaaatcatag ctattctgct tctgttcagt atttcagaat ctctcgaggc tcaaaataaa
      121 gtttctatga agatcgatag tttgctcgat gagtattttg cagtttctaa cagccatcgg
      181 gagctggctc tgtcgctagc agaaaacgcc agaaaattgg cccaaaaaca tggagaaaaa
      241 cataatgaag ccgttgctct tgaatgttta ggggtacaat attattatcg tggagattat
      301 aataaagcac gcgattttct caatcaatcg ttggaaatct ataacgaaga aaactcatcg
      361 acagggctcg ccagtgtttt taccaatctt gccttagtgg aacaggatct gagaaatttg
      421 aatcaggcac attattatta tactacttcg ctggtttacg acagaaacag ccatgatagt
      481 ataggaatgg cctttacaat aaataatctg ggaacactct atctacataa aggagatttt
      541 caacgggcac gcgcttattt tcaaaaagcc tatgatatag gcaaatccgt ccatcatcta
      601 gaagtaattg gaaattccat cggtaatttt ggtctgcttc gcctggaatc ttcaaaccct
      661 gattctgcta ttccttattt caggaaagca ttatctatag caaaacaata tggagatgag
      721 tatgcacaag ctattgcaaa catcaatctt ggcctctgta tgatcgaaaa atcagaagcc
      781 gattcggcag aaagatatct gactatagcc gctcgaacct ccgaagaact ggaagataat
      841 gagttacaaa tagaatgctt attagcaaag gctaaactga tgcttttgct gaaccagccg
      901 gaacttgcca acagaatttt attttcggca caaaaactaa ataatcccac cgaacggaaa
      961 aaaacttcgg cacaaatctt tactctgatc ggaaaaaatc ttttattgat gaatcaaccc
     1021 gagaaagcac gcaactattt tctgcaaagc ctcgacattg cgaaagaaat aggagcactg
     1081 cccgaaatga tagatgccta taaacatctg gccatgtttt attcggccat ttcgatgtac
     1141 gacagctgcg agctttatat cgacctttac gcaaaggaat ggagcaagct gacaatagat
     1201 accacattgc ccgatacatc atttgtatat gaagcaaaat caaacaataa gaatatcttg
     1261 catcgtatat cgaaaaattc acagcttagc cctctaaaat tactcgttac gcttgtcttt
     1321 gccctaacta tcttatgggc aggaatgatt tatttaccta aaatacttct ttcgttgagg
     1381 aagaaaacaa agaaagacaa ttcgaatacg tcggccaaat gattacatat tcaaaacctt
     1441 ttctattttt gtagctgcaa acctatttca actatgaaac tcaaacattc cggttttatt
     1501 ctctttttgg caaccatttt gatattcagt ataacatcct gtgaagatga aacagttcaa
     1561 aggcctgtaa atcaatatca ggtaaaatat gaaatcaaga atcatctcaa aaagaaatgc
     1621 aatttctata tacgctattt caatccaaaa ttcgacaata ttgtggcaga gtattatgac
     1681 tccatcaacg aatggtcgag ggagttgaaa ggaaaaacat tcgaccattt atattttgag
     1741 gcatttacgg taagggatag cgccatgttc gaaatcagta tctatgtaaa tggtcagcaa
     1801 gtggtgagag atgttgacag ttgtccgggg ccatcggtat gcgatacaaa ccggattaaa
     1861 gtctcttacg ttctgcaata gatacgtaaa accctcttta tcagctaaag aagggtaaaa
     1921 aagatgggca agggaaaaat atttcggcaa tttccatcaa ctttctgggc agccaatgtc
     1981 atggaattgt tcgaacgttg ggcatggtat ggtatttata tggttttagc cctctatctg
     2041 acaggttcga ccgatacggg tgccttagga ttcagtcagg aacaaaaagg gagtttgatg
     2101 ggtactgtga cctttttgtt atatctgcta cctatcatca ccggagcgat tgccgacagg
     2161 tatggctata aaaaagttct tactatagct tttatcattc taagcactgg atatttcagt
     2221 ctggctcacg ttagaagtta ttggtcattc tatgttcttt tcctttatct ctcggtagga
     2281 gcagctctat tcaaacctat cataacggcg actgtttcgc atacaaccaa tgatcagacc
     2341 tcatctatcg gatttggaat tttttatatg atcgtaaata tcggtgcctt tttgggcccc
     2401 atctttgcta gcaaacttcg catactcgat tggaaattcg tcttctatct ttcatcggcc
     2461 attatttgca tcaattttat cctattattc tttttctaca aggaaccaat taataaaaaa
     2521 cagcatactc ctatacttgt atctatcaag aacatcatca agaatacggt aacggttctg
     2581 gcagatttca aactaatgct tttcttaata attatggtag gcttttgggc aatgtatttg
     2641 cagctgtttt acactttacc cattttcatc gatcaatgga tggatacaaa aattctatat
     2701 gatgcaatcg aaaatttctg gcctgctttg gcgcatattg caggaacatc gcaaaggacc
     2761 atagcccctg aaattcttac caatatcgat gcattctata tcgtgatatt ccaacttctg
     2821 gtttcgagcc tggtaagccg gttttcgcct ctcagggcga tgaccaatgg tataatgatg
     2881 gcaacaatag gaattgcctt gatgtttgcc tttaataatc cgatttatct tttttttagt
     2941 atattgatct ttagtttggg tgagatggct acttctccaa aaattcagga atatgttggg
     3001 aaaatagccc cacctgataa aacagctctc tatatgggat acagttatct gccacttgcc
     3061 ggcggccata ttgtagctgg cttcatttcg ggctctatat ttgcaaaaat tgccgataag
     3121 tccactctac tacgtaacga tttactttcc agaggaatcg tcatgccaga tatctctcct
     3181 acttataccc taaaccattt ctactccgaa gcctcatcat tgcttcatct cgactcatca
     3241 tgccttactt cttacctttg gcaaacttat catcccggtc gtatctggat attattttca
     3301 tctattggtt tggtttcgtt tttagccttg cttatgtatg ataggttttt cataaggcaa
     3361 aagaaaattc tacaataatg aaaaagtggt ttcccagcat cttccctgcc ctattcatta
     3421 caatattgat gacggctttc tttatatttt cgaattcaag taaaatctcg ttaattcatc
     3481 tgggaattta tcccttaagc gccaaaggac ttttaggtat cgttctggct ccttttatcc
     3541 atgaaaatgc caaccatttg tattccaaca ttattccctt ttttgtactg accatactct
     3601 tgttttattt ttacaaggat attgcctatg agattttagg tttttcatgg atcatgacgg
     3661 gtatactggt ttggttaggg gcacgtccgg cttatcacat tggagcaagc ggagtggtgt
     3721 atgccttaat ggcatttcat gtcttgagcg gaatggtaag aaagaaccca cgcttgctgg
     3781 ccatttcact tcttatagtc tttttttatg gaggaatgat ctggggcatt tttcccgatt
     3841 ttattccagg ccgggctatt agctgggaat cacatctggc gggcacgatt acaggttcca
     3901 tactggctat atattaccgc aaggaagggc cacaaaggcc attatatagc tgggaactcg
     3961 aacccgatga agacgatgag gataatgaag aaaccaaaca aaatacacca tcccaaaatc
     4021 tcgataaaga agccgagaaa gccgaagaaa tcatgataaa atatatttac aaaagaaaaa
     4081 ataccgatga ggagagttcc ggctaaacca tcgaaaatgt aaatccactt tgctaatgca
     4141 caaaatttta taatttcgtg gcaaccaaaa acaattcgaa tgaaaaaaaa ttcactgctt
     4201 tcgtttccgg tcttattggc aatcatgcta tttagctgcc aaaaatccga ccagtatttt
     4261 ctcgagtgca atctcgaggg tttgaatgac ggaatggcct acttgcaggt tattggagaa
     4321 gatggtattc gaacaatcga ctcgacaacc atatcgaatg gaaaatttac cttcaaaggt
     4381 tcacaatccg aacctatttt tgcctatatc actttaaatg gcaaacccga ccgcataggc
     4441 cttttcctgg aaaatgccag agtctcgata tatgggcatg ccgacagttt ggaacatata
     4501 aagatttcgg gttcacccct aaatgatgaa tattcaaaat tcaaatctat gctggcatcc
     4561 tatgaaaatc gccaggaaga attgtatctg caatacaatc aagcacgtaa aaccaatgat
     4621 accttggcac tgaaaaatat cgaaacactg tgggatttac tcgacaagca gcaaaataaa
     4681 gtgattatgc aatttatcag ccagcataag cattcggtca tttctccctt tttgattcat
     4741 caaagactgg tttacaattt aaacgtaaat aaactcgaca gccttacttc gatatttcct
     4801 cccgagctcg aaaactcggt gtatgtacgc atgttaaaga aacgtattca aaccttacgc
     4861 aatgtcgaaa ttgggaaacc cgcaccagaa atcgctctcg acgataccat gggcatttta
     4921 cggactttgt cctctttcag aggaaaatac gtcctgattg atttttgggc atcatggtgt
     4981 agcccatgcc gagaagaatc gcccaatctt gtaaaggcct accaacgttt tcatgacaag
     5041 ggattcgaaa ttttcggcgt atcactggat acacgtcgcg ataaatgggt agaagctatc
     5101 aggaaagatc aactgacatg gacacacgtt tcagacctta aaggctggaa atcggaagca
     5161 gcagaacttt atggggttaa ttccatacct cactccgttc tggtcgatcc ccggggaatt
     5221 atcatacaaa acaatttgag aggtaaagcc ttgatcgatt ttcttgaaaa gctgattaaa
     5281 taaaagatgt ctccatgata gtgcgtttca tttcaaagta atgtccatcg actatctaaa
     5341 atccatcatc ttagataagg ttatgaaagc gaaataaatc agaaagacta cttgtcagaa
     5401 tttcaggcct tatcctacat aataaatgat tggcttaaac ttaatagcac tgaaatatca
     5461 tcaggggctc ccgattccga ttttcacaat ctcacttctt tgagg
//
LOCUS       DAORPD010000011         5444 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_2716_length_5444_cov_2.597328, whole genome shotgun sequence.
ACCESSION   DAORPD010000011 DAORPD010000000
VERSION     DAORPD010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5444
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2716_length_5444_cov_2.597328"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1729
                     /locus_tag="PK976_00285"
     CDS             <1..1729
                     /locus_tag="PK976_00285"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC66357.1"
                     /translation="SNSKTKHQNMKNLRILSLSLLLLLVLPLIAQQPKAQRLVLLEEF
                     TSSTCGPCASVNPTIVQRLQQNPDKFTAIFYHVSWPSPGNDPMYLANTQENNARVNYY
                     GVNSVPYSVIDGNYYTGHPNGWTMTTINNRYAMPSPAEIQLQHYLNAAQDSIFVNMLV
                     ILTDMMTGSQLVAQNVIIEKHIHFNTAPGTNGEKDFYNVMKKMLPGAGGTNLPTPLSP
                     GDYVIMQYSWKLANVYDNNELAAIGFIQNNSSKEVLQTSNSSPAPLTPLYSNDGEILS
                     LSNVAPENCTGKVAPVIRVRNNGSNSLSSITLKYRIDNQPEQEYTWTGNIGFLQSKNI
                     ALPEYLFAPQNSNTLKIYIDKVNQLQDEYRKNDTLTFHLSEPKTATTVLNIWIKTDNK
                     PEEITWSIKTSDGSLVSSGGPYAQASTLIKETIKVESEHCYQFALYDAGGNGLCCANG
                     LGFFTLFDDKNVTIAEGTTFGSEVLAQFYSQSGIGIEDLSKQYLSIIPNPVSHLSMIY
                     FNMNTDGKVNLNIYNLNGSLIFQKVSETLNKGEQKMKLNVERMNSGIYLIEIIMPDKK
                     VLRQRFVVQ"
     gene            complement(2173..3966)
                     /locus_tag="PK976_00290"
     CDS             complement(2173..3966)
                     /locus_tag="PK976_00290"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF011531.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HRC66358.1"
                     /translation="MDNFIVSARKYRPSTFDEVVGQQSIVQTLKNAIRTRQIAQAFLF
                     TGPRGVGKTTTARIFAKTINCQNLTPNFEPCNHCDSCKDFNNNTSLNIFELDAASNNS
                     VDNIRSLIDQVRIPPHHGKYKVYIIDEVHMLSQNAFNAFLKTLEEPPDYVKFILATTE
                     KHKVMATILSRCQVYDFKRITTEDIVHQLQHVAQNEGISYEVEALHVIAEKADGAMRD
                     ALSIFDQLVTYSGCHLTYQKVAESLYVLSYEDYFKMSEDLLGGKISDALILLNDIIER
                     GFEGQMFINGLGKHFRNLWLAKDPASVGLLEVSETTRNFYLTQAQKCSLQFLAKALNL
                     INQCDANYRMSLNKRLAIEITLINICQIENPFTNSKPKAPTTPSLTTEPNAQYSPKSS
                     PAATAAPSSSLSQKSSSLSPQVPKTSASPSQLPKSSPNNGNDSPKNGLSHFGLQTANL
                     RKISNPVEEKKSEKTEENQKHEQQIHDFTEEELLIKWTSFLDALNEKEGALVCSLRMA
                     KPRKMGNNTIVFRVFNSVIENDILSNQDRILDFIRSELSNSQIKLITEITASSENNIK
                     AYTPLERLKELTQRNPAVNEFMQKFNLQPEL"
     gene            4186..>5444
                     /locus_tag="PK976_00295"
     CDS             4186..>5444
                     /locus_tag="PK976_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRC66359.1"
                     /translation="MKTLWGIEHEFTLNEDEFNAWQGPKMIYGGSFQENEVWFDAVSL
                     LFERNIAGVDTGKYSPYTGLFPVYGSDSALPFDAFASTFYMLSRYEEYLPNRKDTHGR
                     FPATESIAYREGFLDKAVVNRWAEMIVAILLKRFPDLHFHKTSYQYVPTIDVDLAYSY
                     LHKGFLRTLGGYVKSLIHADFEDVRSRTHVLMGKERDPFDSFDALHHLHSTYYLKPVF
                     FFLFSDYGQYDKNLSIYNPAFRNLIKKVADYATVGIHPSYACYENPQKLKKEIEDLSQ
                     CLHRQITHSRQHFLRLNFPETYRNLIHCGITDDYSMGFADQPGFRAGTCNSFPFYDLD
                     LDTATSLLIHPFAFMDGTLKDYLKIPKDQVVDFVKPYIEEVKRYSGTLITLWHNETHG
                     GSGRWEGWPEIYETIVKMGASSSIETST"
BASE COUNT         1650 a         1045 c         1098 g         1651 t
ORIGIN      
        1 atcaaattca aagaccaaac accaaaacat gaaaaattta cgaatcctca gtctcagtct
       61 attgctgctt ttagtgcttc cgctgatagc acagcagccc aaagctcaac ggctcgtatt
      121 gcttgaagag tttacctcat caacctgcgg accatgtgct tcggtgaatc caactattgt
      181 tcagcggctg caacagaacc ccgacaagtt taccgccata ttttaccatg taagctggcc
      241 ttcaccgggg aacgatccta tgtatctggc caatacccag gaaaataatg caagagtaaa
      301 ctattatggg gtaaattcag tcccttattc ggtgatcgat ggaaattatt atacggggca
      361 tcccaatgga tggactatga ctaccatcaa taatcgttat gccatgccct cacctgccga
      421 aattcagctt caacactatc ttaatgctgc ccaggacagt atcttcgtga atatgctggt
      481 gattcttaca gatatgatga caggcagtca attggttgca caaaatgtaa tcatcgaaaa
      541 acatattcat tttaatacag ctccgggaac taatggcgaa aaagatttct acaacgtgat
      601 gaaaaaaatg cttcctggag ccggtggaac taatttgccc actcctcttt cgcctggtga
      661 ttatgttatt atgcaatatt catggaaact ggccaatgtt tatgataaca acgaacttgc
      721 cgccattggc ttcattcaga ataattcctc gaaagaggtt cttcaaacat ccaacagctc
      781 tcctgcacct cttactccgc tttatagcaa tgatggagag attctctcct taagcaatgt
      841 agcgcccgaa aattgtacgg gaaaggttgc tcctgttatt cgcgttcgaa ataatggttc
      901 caattccctc agttctatta cattaaaata tagaatagac aaccagcctg aacaggaata
      961 tacatggacg ggcaacattg gatttctaca atcgaaaaat attgctttgc ctgaatattt
     1021 gtttgcaccc caaaattcga atactcttaa aatttatatc gataaggtca atcaattaca
     1081 ggatgaatat cgtaagaatg acacccttac cttccatctt tcagaaccta aaactgcaac
     1141 aacggttctg aacatatgga ttaaaaccga caataaaccc gaagaaatca catggagcat
     1201 caagacaagc gatggcagtc tggtaagctc aggcgggcct tatgcacaag ccagcaccct
     1261 gatcaaagaa actattaaag tggaatccga acattgctat caatttgctc tttacgacgc
     1321 tggaggaaat ggtttgtgct gtgccaacgg attgggcttc tttactcttt ttgacgataa
     1381 aaacgtaacc attgccgaag gtactacctt tgggagtgaa gtactcgctc agttctattc
     1441 gcaaagcgga ataggaatcg aagatttatc gaaacaatat ctatctatca ttcctaatcc
     1501 ggtgagtcat ttatccatga tctactttaa tatgaataca gatggaaaag taaatctcaa
     1561 tatctacaac ttgaacggaa gcctcatctt tcaaaaggtt tcagaaacat tgaacaaagg
     1621 cgagcaaaag atgaagttaa atgttgaacg aatgaattct ggtatttatt tgatagaaat
     1681 tataatgccc gataaaaagg tgttgcggca gcgttttgtc gtgcaataag tcctaatact
     1741 catctcattt ttttcatgac tcttataata aaaacaggct gccctcaaaa gtagcctgtc
     1801 ctttattact aaacaaaatc cattaaatgt taaccaaaac catttaattg gaaacaatta
     1861 atatgccagg gtttaatact aaaagaaaat aatcataatt aattttatat tattattaac
     1921 caatatctta cgtttatatc atcagttcga actctctgga acccttgccg ccactgaaaa
     1981 gtgttattat atgtttcgat tttcaaaaat acatgcaata aatcttactt taaggaaata
     2041 ccctatgaat tccccaaaaa acgataaaaa aaagacagaa cagggccata agaaataagt
     2101 ccggctgttt cccgataact tgcatcaaac aaattaatta aaacttcgaa aaaaaatcga
     2161 tttcgatgaa agttacagtt caggctgaag gttgaatttt tgcatgaatt cgttgacggc
     2221 tggatttcgt tgcgtaagtt cctttaatct ttccaaagga gtgtatgctt taatattatt
     2281 ctcggatgaa gcggttattt cggtgatgag ttttatttga gaattagaga gttccgatcg
     2341 aatgaaatct aagattcggt cttggttact gagaatatca ttttctatta cactattaaa
     2401 cacccgaaat acaatagtat tatttcccat cttgcgcggt ttggccattc taagcgaaca
     2461 aaccaatgct ccttcttttt cattcaaagc atcgagaaag gatgtccatt ttattaagag
     2521 ttcttcttct gtgaagtcat gaatttgttg ttcatgtttc tgattttctt cagttttttc
     2581 cgattttttt tcttcgacgg gatttgaaat ttttctcaag ttagcggttt gtagcccaaa
     2641 atgggagagc ccgttctttg gagaatcatt tccgttgttt ggggaagatt tgggaagttg
     2701 ggagggagag gctgaggttt ttggcacttg gggagatagg gaggaagatt tttgagaaag
     2761 agaagaagaa ggggcagcag tggcagcggg tgaagattta ggggaatatt gagcattagg
     2821 ttcggtagta agggaagggg tggttggcgc cttgggtttc gagttggtaa aaggattttc
     2881 gatctggcaa atattaataa gagtaatttc gatggcgagt cttttattga gactcatgcg
     2941 gtagttagca tcgcattggt tgatgagatt gagtgccttg gccagaaatt gaagagaaca
     3001 tttttgggcc tgtgtcagat aaaaatttct tgtcgtttcc gaaacctcaa gcaatccaac
     3061 cgaggcgggg tctttagcca accaaagatt acgaaaatgt ttgcccaacc cattgataaa
     3121 catttggcct tcgaagccac gttcgatgat atcattcaat aaaataaggg catcgctgat
     3181 ttttccgcct aacaaatcct cactcatttt gaaataatcc tcataactaa gcacataaag
     3241 gctttctgcc actttctggt aggtgagatg acagcccgaa taggtaacca gctgatcgaa
     3301 gatggaaaga gcatcgcgca tagctccatc ggccttctcg gcaatcacat gcaaggcctc
     3361 cacttcataa cttatgcctt cattttgagc aacatgttgg agctggtgga cgatatcctc
     3421 agtagtaata cgcttgaaat cataaacctg gcaacgtgaa agtatagtgg ccattacctt
     3481 gtgtttttcg gttgtagcta gaataaactt tacataatcg ggaggttctt caagtgtctt
     3541 taagaatgca ttgaaggcat tctgcgacag catgtgtact tcatcgatga tgtacacctt
     3601 atattttcca tgatgtgggg gaatacgcac ctgatcgatc agtgaacgga tattatctac
     3661 cgaattgttg gaagcggcat ccaattcgaa aatattcaat gaggtattat tattgaaatc
     3721 tttacacgaa tcacaatgat tgcatggttc gaagttgggg gtgagattct ggcagttgat
     3781 ggttttggca aagattcgtg cagtggttgt tttacctacg ccacgcgggc ctgtaaataa
     3841 aaaagcctga gcaatttgcc gagtacgtat agcatttttc aaggtttgta ctatactttg
     3901 ttgccccact acttcgtcga aagtgcttgg cctgtatttc cttgctgaaa caataaaatt
     3961 atccatgctg taaataaaaa gaatctggcg tcaaacttaa tgctttttta ccaaatgaat
     4021 atatgttggg cctttgtacc tttgtggttt tcattcagtg taactttcgc aagtttttaa
     4081 tcgaaaacat tttattttag aatatttatg ggcaattctg gcgacagatt actgataaaa
     4141 atcccaaaat acagcaagcg acttcaatat acatttcagt ttctgttgaa gacgttatgg
     4201 gggatcgagc atgagtttac gctcaatgag gatgaattca atgcatggca aggtccgaag
     4261 atgatttatg ggggaagttt tcaggaaaat gaagtttggt tcgatgcggt ttccttatta
     4321 ttcgagcgaa acattgcagg ggtcgacacg ggaaaatatt cgccctatac cggtcttttc
     4381 cccgtttatg gttcagactc ggctttgcct ttcgatgcat ttgcctcgac cttttacatg
     4441 ttgagccgtt atgaagaata ccttcccaat cgaaaagata cacatggacg ttttccagct
     4501 acagaaagta ttgcctatcg tgaaggattt ctcgacaaag cggttgtgaa tcgttgggca
     4561 gaaatgatag tagctatctt attaaaaaga tttcctgatt tgcatttcca taaaacttcc
     4621 taccaatatg ttcccactat agatgttgat ttagcttata gttacttgca caaaggattt
     4681 ctaagaactc tcggaggata tgtaaaaagt ttgatacatg cagattttga agacgtaaga
     4741 agccgaacgc atgtattaat gggcaaagaa cgtgatcctt tcgatagttt tgatgccttg
     4801 catcacttgc attccaccta ttacttaaaa ccagtatttt tctttttgtt tagtgattat
     4861 ggacaatacg acaagaattt gtcgatttat aacccagctt tcagaaactt gatcaagaag
     4921 gtggccgatt atgctacggt aggaattcat ccttcgtatg cttgctatga aaatcctcag
     4981 aaattaaaaa aagaaatcga agacctatcc caatgcttgc ataggcaaat tacacatagt
     5041 cgtcagcatt tcttgagatt gaattttcct gaaacctatc gcaacctcat ccactgcgga
     5101 attaccgatg attattcaat gggttttgct gatcaacctg gattcagagc cggtacctgc
     5161 aattcctttc ctttctacga cctcgatctg gatacggcca catctttgtt gattcatcct
     5221 tttgccttta tggatggaac tctaaaagat tatttgaaga ttccaaaaga ccaggtagtt
     5281 gattttgtta agccatatat agaagaagta aagcgttatt caggtacatt gattactcta
     5341 tggcacaatg aaactcatgg gggcagtgga cgatgggaag gctggcccga aatatacgaa
     5401 accattgtga aaatgggagc ttcatcttcc attgaaacat ccac
//
LOCUS       DAORPD010000012         5439 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_2721_length_5439_cov_2.254829, whole genome shotgun sequence.
ACCESSION   DAORPD010000012 DAORPD010000000
VERSION     DAORPD010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5439
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2721_length_5439_cov_2.254829"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1296)
                     /locus_tag="PK976_00300"
     CDS             complement(<1..1296)
                     /locus_tag="PK976_00300"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HRC66360.1"
                     /translation="MKRTKNLIDLLVVAILISVPYLVNSQNFVPYSTTLPNVAQGSCD
                     WADIDNDGYLDLAMMGANSIGQPLTKIFRNNAGVFIDIDANLIGLKNGSLRFGDYDRD
                     GDYDLLVCGSDFSSKPHSLLYKNDNGSFSQSSNTFQGIYNGMAIFVDYDNDGLLDIFL
                     SGDTLSYKGFTRIYHNLGNDNFEEVDFGFPGVISSSLSYGDYDNDGDFDLLLSGDIGG
                     AYYTKLFRNDMGLFTEIPTNIEGVGAGFSLFMDFDKDGDHDILLMGNDLTLTPIFKIY
                     RNDGNGSLQEVFVGITGLALGNISIADYNSDGYPDFATTGKAPGCGAMMSVLFYNNQI
                     GSFWPSSQNLVDLSYSYSSWADFDNDGDSDLLLMGISTSGNPETVLYSNTLNSNGFVP
                     NLPPFAPIDFSSEMNGNELILKWKPSPYNTNGKNIFYNVR"
     gene            complement(1636..2838)
                     /gene="pcaF"
                     /locus_tag="PK976_00305"
     CDS             complement(1636..2838)
                     /gene="pcaF"
                     /locus_tag="PK976_00305"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: protein motif:HMM:NF006551.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HRC66361.1"
                     /translation="MKEAFICDAIRTPIGRYGGTLASIRTDDLAVIPIKALMERNASV
                     NWEEVDDVIFGCANQAGEDNRNVARMALLLAGLSERIPGVTVNRLCGSGMEAIGQAMR
                     MIRTEDADLIIAGGVESMSRAPFVMGKASEAFSRDVQIYDTTIGWRFINKKMEQLYGV
                     DSMIETAENVAGMYHISREDQDIFAYHSQLKAHQAIQSGAMEREITPVEIVRPKSEPF
                     VFSMDEHPRLSSLEKIGTLRPVSRPGGTVTAANSSGVNDGAAALLIASENALKKYKLR
                     PMARIVGVQAAGVPPRIMGIGPVPATQKLLSKLHFSLEDFDLIELNEAFASQSLACMR
                     LLGLKEDDPRINPQGGAISLGHPLGMSGARLITTAVHQMSNNNLHRALCTMCIGVGQG
                     IASIIEKV"
     gene            complement(2853..4184)
                     /locus_tag="PK976_00310"
     CDS             complement(2853..4184)
                     /locus_tag="PK976_00310"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HRC66362.1"
                     /translation="MDSLKEEKNLEESLYERALKVLPGGISRNVIFRQPHPHYVASAH
                     GCYVEDVNGITRIDFVNNIASLVHGHAHPAIVKAVYEQLQKGTAYSMGTEAEIRHAET
                     LCHRVPGFEKIRFMNSGTEAVMSLIKASRAYTGKHKIAKAEGAYHGSYDFAEVSESAS
                     PDNWGPVEQPNSVPHVRNTPPSVLDEVIVFPYNDVERTLAILDRHADEIACVLIDPVP
                     HRIGMIRATDEFVEAVYDWTRKHNTLMAFDEVVCFRINYEGAQALYRVKPDLTALGKL
                     IGGGFPVGALTGKAEIMDVLNPLQTPLRFSLSGTFSANPITMTAGRVAVEMFDKQAVD
                     KVNHLAQEAKEQLMQAAKIAGIPLSVAGAGSMMKMHFRETLPSNYRECYEDANHKKTV
                     NTFLDFMYDHGIILVYSNSCFFSTVMKQEHIDKLSETALEGFKFIKPLLNV"
     gene            complement(4211..4861)
                     /locus_tag="PK976_00315"
     CDS             complement(4211..4861)
                     /locus_tag="PK976_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012458079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HRC66363.1"
                     /translation="MVNIKGWSNDEIARKIAIDIPDGSYVNLGIGLPELVAANIPEGK
                     EIIFHTENGLLGVGPPPPPGQEDDELINAGKKPVTAIPGACFFHHADSFAMIRGGHID
                     FCVLGAFQVSESGDLANWSTGELNAIPAVGGAMDLVAGVPNIFIIMQHITKTGQPKIV
                     RQCTYPLTGRQVVKTIYTNLAVIDVTPHGLEVREIHSGMDRETLQNLTEASLSFRS"
     gene            complement(4855..>5439)
                     /locus_tag="PK976_00320"
     CDS             complement(4855..>5439)
                     /locus_tag="PK976_00320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit A"
                     /protein_id="HRC66364.1"
                     /translation="GAKNLTVISNNTGNGHVGLAALIENLQVKKMICSFPRTAQSVVF
                     PELYAKGMIELELVPQGTLAERIRAGGAGIPAFYTPTTIGTPLAEGKEIRHIDGKSYV
                     LEYALKADFALIKCKTADRYGNLLYNKTARNFGPIMCMAAKTSIVQTSSLVEPGEIDP
                     ENVVTPGIFVKRVVKISNPLSENQLLRENRRYPW"
BASE COUNT         1526 a         1241 c         1082 g         1590 t
ORIGIN      
        1 ccttacatta taaaatatat tcttaccatt agtattatat ggagatggtt tccatttcaa
       61 tatcaactca tttccattca tctccgatga aaagtcaata ggagcgaagg ggggcaaatt
      121 tggaacgaat ccattcgaat tcagggtatt agagtaaaga acggtttcag gattcccgct
      181 ggtagaaatt cccatcaaca ggaggtcgct atcgccgtca ttatcgaaat cggcccaact
      241 agaatagcta tatgacaaat ctacaaggtt ttgagaggaa ggccaaaaag aaccaatttg
      301 attattgtag aacaagacgc tcatcatggc tccacaacca ggcgccttgc ccgttgtggc
      361 aaaatcagga tatccatcgc tattgtaatc ggcaattgaa atgttaccca atgccaaacc
      421 cgtaattcca acaaacacct cctgtaaact accatttcca tcatttctgt atattttgaa
      481 aattggagtc agcgtcaagt cattgcccat cagcaaaata tcatggtctc cgtccttgtc
      541 gaaatccatg aacagtgaga accctgcgcc tacaccttct atattggttg ggatctcagt
      601 aaatagaccc atatcgttgc ggaatagttt ggtataataa gctcctccaa tatctccact
      661 tagcaaaaga tcgaaatcgc catcattgtc ataatctcca taactaagcg atgaactgat
      721 gactccagga aaaccaaaat ctacttcttc gaagttatca tttcccaagt tatgataaat
      781 tcgtgtaaat cctttgtaag aaagcgtatc tccacttaag aatatatcaa gaaggccgtc
      841 attgtcataa tccacaaaga tggccattcc attataaatg ccttggaatg tattggatga
      901 ttgcgaaaag ctaccattat cgtttttata aagcaaacta tgaggctttg agctgaaatc
      961 gcttccacaa accaacaggt cgtagtcgcc atctctatca taatcaccaa atcgtagcga
     1021 gccatttttc aatccaatta ggttggcatc gatatcaata aacacaccgg cattgtttct
     1081 aaagattttt gttaaaggtt ggccaataga atttgcgccc atcattgcca agtcgagata
     1141 gccatcattg tctatatctg cccagtcaca actgccctga gcaacgttag ggagggtagt
     1201 cgaataggga acgaaattct gtgaattcac taaatacgga actgaaatta atattgcaac
     1261 gactaaaaga tctatcaggt tttttgttct tttcataaaa attctcgatg aaatttaata
     1321 aaattttaaa tctataagtc caaccgaaga ggaaacaccc gataggggca atttattgct
     1381 atcgcgcatg caatttatta ttttgtttcg tatgttaaat cgatttacag acagatagtt
     1441 cggtaaacag ggcagcagtt ttctgttttc atggtaatcg ttgaacatgc ggaagttgac
     1501 ataagtggta atgcccatta ttttttcatt cgaatcgatc gtatctgaaa agtccatgat
     1561 aggtctccat tctttgtttt ttataataga tttttcatga accgtcttct aaaaaagtat
     1621 ttaaagaatt ttcgtttata ctttctcgat aatcgaagca attccctgcc ccacaccaat
     1681 gcacatagtg cacaaagctc tatgaagatt gttgtttgac atttgatgta cagctgtagt
     1741 aatcaggcgg gcgccactca ttcctaaagg atgccctaag gatatggctc ctccctgcgg
     1801 attaatccgg ggatcatctt ccttaagccc taacaatcgc atgcaggcaa gcgattgtga
     1861 ggcaaacgct tcgttcagct caataaggtc gaagtcctcg agagaaaaat gaagtttgga
     1921 aaggagtttt tgcgtagctg gtacaggacc aattcccata atacgaggag gaaccccggc
     1981 ggcttgcacg ccgacgattc tggccattgg acgcagctta tattttttca gagcattttc
     2041 agaagcaatc aataaggcgg cggctccatc attcactcca gatgaattgg ctgccgtaac
     2101 tgtcccacca ggcctcgata caggtcttaa agttcctatt ttttctaagg aagataaacg
     2161 tggatgttcg tccattgaaa aaacaaaagg ttcagattta ggccgaacaa tttcaactgg
     2221 cgtaatttcc ctttccatag ctccactttg aatggcctga tgtgccttaa gctgactatg
     2281 ataagcaaaa atatcctgat cttcccggct aatatgatac attcccgcta cattttcggc
     2341 ggtttcaatc atggaatcca ccccatagag ttgttccatt tttttattga taaatctcca
     2401 gcctatagta gtatcataaa tttgaacatc tctcgaaaag gcttccgatg ccttacccat
     2461 gacaaatgga gctcttgaca tgctttctac gccaccagct attatcaaat cggcatcttc
     2521 cgttcgtatc atgcgcatag cctgtcctat ggcctccatt cccgaaccgc acaatctatt
     2581 gactgttacg ccgggaattc tttcgctcaa gcctgccaga agcaaggcca ttcgcgccac
     2641 attacgattg tcttcaccag cctggttggc acatccaaaa attacatcat ctacctcttc
     2701 ccagttcacc gaagcatttc tctccatcag ggccttgatg ggaatcacgg ccaaatcatc
     2761 ggttctaatg gaggccaatg ttcctccata gcgtcctata ggcgtgcgaa tagcatcaca
     2821 gatgaaagct tctttcatgc ttcgtcgttt aatcaaacat tcaacagggg cttgatgaac
     2881 ttaaagcctt caagtgcagt ttccgaaagc ttatcgatat gttcctgttt cattaccgtg
     2941 ctaaagaaac atgaattgga atagacaaga ataattccat gatcatacat aaaatcgaga
     3001 aatgtattca cggttttttt atggttggca tcttcataac actcgcgata atttgagggt
     3061 agtgtttctc taaaatgcat tttcatcata gagcctgcac cggcgacgga taatggaatt
     3121 ccggcaattt tagccgcttg catcaattgt tctttggctt cctgagccag atgatttacc
     3181 ttatctacag cttgtttgtc gaacatttcc actgctacgc ggccagcggt catggtgatg
     3241 ggattagccg agaaggtgcc agaaagagaa aaccgcaagg gggtttgtaa aggattaaga
     3301 acatccataa tttcagcttt accagttaat gctccaacgg ggaaaccacc ccctatcaat
     3361 tttcccaaag ccgttaagtc gggttttact ctataaagtg cctgagctcc ttcgtaattt
     3421 attctaaaac acaccacttc atcgaaagcc atcaatgtat tgtgttttct tgtccaatca
     3481 tacactgctt ccacaaattc atctgtagcc cgaatcattc ctatacggtg aggcacggga
     3541 tcgattaata cacaggcaat ttcatcggca tgccggtcga ggatggccaa ggttctttcc
     3601 acatcattat agggaaagac aattacctcg tcgagcacac tgggcggagt attccttacg
     3661 tgaggtacac tgttgggttg ctcaacaggt ccccaattat caggagaagc cgattcacta
     3721 acttcagcga aatcgtagct tccatggtaa gctccttcgg ctttagcaat cttatgtttt
     3781 cctgtgtaag ctcttgatgc ttttatcaac gacatcacag cctcggttcc gctattcatg
     3841 aacctgattt tttcgaatcc tggaactcga tgacaaagtg tttcggcatg acgaatctct
     3901 gcttcggttc ccatcgaata tgcagttccc ttctgaagct gctcataaac agcttttaca
     3961 atggcaggat gtgcatgacc atgaacgaga gaggcaatat tgttgacaaa atcgatacgt
     4021 gttattccat tgacatcctc gacatagcag ccatgagccg aagccacata gtgcggatgt
     4081 ggctgacgaa agattacatt tcggctgata cctcctggaa gaaccttcag ggcacgctca
     4141 taaaggcttt cttctaaatt tttttcctct ttcaatgaat ccatgataat cgtaaatttt
     4201 atgaataaaa ctatgatcga aaactaagac tggcttcggt gagattttgt aaagtttctc
     4261 tatccatgcc ggaatgaatt tccctcactt cgagtccatg aggggttaca tctatcacag
     4321 ccagattggt atatatggtt ttaaccactt gccttccggt gaggggatag gtgcattgtc
     4381 tcactatttt aggctggccg gtctttgtaa tatgctgcat gatgatgaaa atattaggaa
     4441 cgccagccac aaggtccatg gcgccgccca cggcaggtat ggcattaagc tcgcccgtgc
     4501 tccaatttgc caggtcacca ctttccgata cttgaaaagc acccagtacg caaaaatcaa
     4561 tgtgccctcc tctaatcatg gcaaaactgt cggcatgatg gaaaaaacat gctcctggga
     4621 tagccgttac gggtttcttg ccagcattga tgagttcgtc gtcttcctgt ccgggaggtg
     4681 gtggaggtcc tacccccaat aatccatttt ctgtatgaaa gataatctct tttccttcgg
     4741 gaatattagc agccaccagt tcgggaagtc ctatgcccag atttacataa gaaccatcgg
     4801 gaatatcgat ggcaattttg cgagcaattt catcattgct ccatcctttt atatttacca
     4861 tggatacctc ctattctccc tgagtaattg gttctcactc aatggatttg aaatttttac
     4921 gactcgtttc acgaagatgc caggtgtaac tacattttcg gggtctattt cgccaggttc
     4981 gactaaactc gaagtttgaa caatgcttgt tttagcggcc atacacataa tagggccgaa
     5041 atttcgggct gtcttgttat agagcagatt gccataacga tcggcggttt tgcatttgat
     5101 tagagcaaaa tcggccttga gagcatactc cagaacgtag gatttcccat cgatatgtcg
     5161 tatctctttg ccttctgcta gtggagtacc aatggtggtg ggggtgtaaa aggccggtat
     5221 tccggccccg cccgcgcgta ttcgttcagc aagtgtaccc tgtggcacaa gctccagttc
     5281 tatcatccct ttagcatata attcaggaaa aactaccgat tgggcagtgc gtggaaagga
     5341 acaaatcatc ttctttacct gaagattttc gatcaatgca gccaatccca catgcccatt
     5401 acctgtatta ttactgatga ctgtcagatt tttagctcc
//
LOCUS       DAORPD010000013         5439 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_2722_length_5439_cov_2.209695, whole genome shotgun sequence.
ACCESSION   DAORPD010000013 DAORPD010000000
VERSION     DAORPD010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5439
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2722_length_5439_cov_2.209695"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1285)
                     /locus_tag="PK976_00325"
     CDS             complement(<1..1285)
                     /locus_tag="PK976_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66365.1"
                     /translation="MSEEKLSVRLSKIATEFNVGISTIVEDLAKKGHKIENNPNTRIP
                     AELYDLLRKLYESDKKIKESVEKKSISSIKKEIPSTETRKIKEETSADTESESIIIKE
                     NVIRPPTPPSVKSTIVQPETGSTAFTEKPPIQETIPTTPELETEENSIRSTVQEIIPG
                     PKIVGKIDLEKQKPSKKSTKTATPKVSGKKSPKLQTETPVIEEKKIEEVSTIKEEDEG
                     PPPSLEGKTIQPPTLEQPAEIQTKAEIEEQAGVLESIQKDEPEVQRKIKTETEILEAQ
                     KTRQEVEKIPSSMVKVEPEITEPIPVKKIEPEEKTTKPDNFIPTKVKKLPGPTIIGKI
                     ELEQVKGGKLTGKEPVATSSEVVGKKSKKKKRKRISGESLLEKATTPKSQTPVETHKP
                     VVHKARKRKDKSKDYRGVKEEVKPELTEEEIQKKVK"
     gene            complement(1416..2648)
                     /gene="nusA"
                     /locus_tag="PK976_00330"
     CDS             complement(1416..2648)
                     /gene="nusA"
                     /locus_tag="PK976_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HRC66366.1"
                     /translation="MEHNLITSFTEFKDYKNIDRETLMRILEDVFRHTLIKKFGTDEN
                     FDIIVNIDKGDLEIWRTLTIVEDGNLTDPNKEIEYSEAIKLEPDFEIGEQVYQEIKLE
                     DFGRREILTIRQNLISKVQEYEKDNIYRKYKDKVGEIITGEVYQIWKKEILVLDDEGI
                     ELSLPKSEQIPHDHYHKGDTIRAVVAKVELRNSQPVIILSRTSPLFLEKLIEQEVPEV
                     YDGLITIKKIVRVPGERAKVAVESYDDRVDPVGACVGMKGSRIHGIVRELRNENIDVI
                     NYTSNPSLFIQRALSPAKVSSIVIDEENKRAEVYMKPDQVSLAIGKGGYNIKLAGKLT
                     GYEIDVYRDSDAEYEDVDLQEFADEIDQWIIDTFKSIGLDTAKSVLNESVEELIRRTD
                     LEEETILEVRKILQAEFE"
     gene            complement(2677..3120)
                     /gene="rimP"
                     /locus_tag="PK976_00335"
     CDS             complement(2677..3120)
                     /gene="rimP"
                     /locus_tag="PK976_00335"
                     /inference="COORDINATES: protein motif:HMM:NF002531.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly cofactor RimP"
                     /protein_id="HRC66367.1"
                     /translation="MEIIDLYLEGSDKFLVDLKIKPGNKILVFIDGDHGINIEDCATL
                     SRFIESKLNRDEEDYSLEVSSTGADAPIRLKRQYKRHIGRKFKIILTDKHQVRGELQR
                     VEPEGIVLNIQETIITEKGKKKIHFIEKFFPFENIEEARIELSFK"
     gene            3370..4521
                     /locus_tag="PK976_00340"
     CDS             3370..4521
                     /locus_tag="PK976_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRC66368.1"
                     /translation="MPRILVVDDERAIRNTLREILEYEKYMVDEAQNGQEAIEKVQSN
                     DYDLVLCDIKMPGIDGIEVLDKILEQGETPVIMISGHGTIDTAVEAIKKGAFDYISKP
                     LDLNRLLVSVRNALDRSRLLTETRNLRKRVLKNNQIIGESNAIKNVLAMLERVAPTNA
                     RVLITGENGTGKELVARALHENSPRAHNPFVEVNCAAIPSELIESQLFGHEKGSFTSA
                     IKQRKGDFELANGGTLFLDEIGDMSLSAQAKVLRSLQENKITRVGGEKEIPVDVRIIA
                     ATNKDLKAEIAKGNFREDLYHRLSVIIIHVPSLAERTEDIPLLAKHFMEIISAEMGKP
                     PLEFTPDSLEALKKRKWTGNVRELRNMVERLAILCDRVITAEDVIKYSN"
     gene            complement(4518..5189)
                     /locus_tag="PK976_00345"
     CDS             complement(4518..5189)
                     /locus_tag="PK976_00345"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HRC66369.1"
                     /translation="MLQRKFEDNYRHKGLRKKLVEEIRRKGITSENVLKAIEEIPRHW
                     FLDVAFLEFAYQDKPFPIGYQQTISQPYTVAFQTQLLDIQPMEKVLEIGTGSGYQACV
                     LSKLGAKVFTIERQKKLYQQTKEFLQAAGFSDIRCFYGDGYKGLPAYAPFDKIIITAG
                     APEIPQALIEQLKPGGRLVVPIGKNSMQIMTLLIKNADGSLTKEEHGFFAFVPMLRNK
                     ANDDL"
BASE COUNT         1396 a         1018 c         1126 g         1899 t
ORIGIN      
        1 cctttacctt tttttgaatt tcttcttctg tcaattcagg cttgacctct tcttttactc
       61 ccctgtaatc ctttgacttg tctttcctct ttcttgcctt atgaaccaca ggtttatgag
      121 tttcgacagg agtttgagat tttggtgttg ttgccttttc caggaggctt tcgccgctga
      181 ttcttttcct tttcttcttt ttcgatttct tgcctacgac ttctgatgag gtggcaacag
      241 gctccttgcc ggtaagtttg cctcccttga cttgttcaag ttcgatcttc cctataatag
      301 tagggccagg taactttttt actttggtag gaatgaaatt gtcgggtttg gttgtttttt
      361 cctctggttc tatctttttc accggaatag gttctgttat ttcgggttca acctttacca
      421 tggagctggg aattttttcg acttcttgcc tggttttctg tgcttcaaga atctcggttt
      481 cggtttttat ctttctttga acttctggtt catctttctg aatggattcc aaaactccgg
      541 cctgctcctc tatttcagcc ttggtttgta tttcggcggg ttgttccagt gtaggtggtt
      601 gaattgtttt gccctctaat gaagggggag gcccctcgtc ttcttccttg attgtagaaa
      661 cttcctcgat ttttttctct tctatgactg gagtttccgt ctgtagtttt ggagattttt
      721 tccctgaaac cttgggtgta gctgttttag tgcttttttt agatggtttt tgtttttcga
      781 gatcaatttt gcccacgatt tttgggcctg gtataatctc ttgaacagtt gatcgaattg
      841 aattttcttc ggtctctaac tctggagtag ttggtatggt ttcttgtatt ggtggctttt
      901 cggtaaaagc agtagaacca gtttcaggtt ggacaatagt agattttacc gaaggaggtg
      961 taggaggtct gataacattt tctttaataa tgatactttc actttcagtg tcggctgagg
     1021 tttcttcttt tatcttcctg gtttctgttg agggaatttc ttttttaatg gaggagatac
     1081 tttttttctc tacggattct tttatttttt tgtcagattc atacaatttg cgcaataaat
     1141 catataattc ggcaggaatc ctggtgttgg gattattttc gattttatga cccttcttag
     1201 ccaggtcttc gacgatggta ctaatcccta cattaaattc cgtcgcaatt ttactcagcc
     1261 tcactgatag tttttcttct gacataaatt ttatttatga tacgctataa tttttgcaaa
     1321 attagtcgat taatatgaaa tatcgggtta ttatcatatt tctatgggtt ttggtgaata
     1381 ggagtgatta ttactaaaca tattcgtaaa ttttattatt cgaattcggc ttgcagtatt
     1441 tttctaactt cgagaatagt ttcttcttca aggtcggtgc ggcggatcag ttcttccaca
     1501 ctttcgttta gaacgctttt cgcagtatcg aggccaatag atttaaaggt atcgatgatc
     1561 cattggtcaa tttcatcggc aaattcttgc aaatcaacgt cttcgtactc agcatcgcta
     1621 tcacgataaa cgtctatttc atagcctgta agctttcctg ctagtttgat gttataaccg
     1681 cctttcccga tggcaagcga aacctggtcg ggtttcatgt aaacttcggc tcttttgttt
     1741 tcttcgtcga tgacgatgga tgagactttg gctgggctta aggctctttg gatgaaaagg
     1801 cttggattgg aagtataatt gattacatcg atgttttcgt ttcggagttc acgtacaatt
     1861 ccgtgaatac gcgagccttt catccccaca catgcgccta caggatcgac gcggtcgtcg
     1921 tatgactcta cggccacttt tgccctttct ccaggtactc tcacaatttt cttgatcgta
     1981 atcagtccat cataaacttc cggtacttct tgctcgataa gtttttcgag aaacaggggc
     2041 gatgtacgtg aaaggattat aactggctgg ctattgcgca gttccacttt tgcaactaca
     2101 gcacgtatgg tatcgccttt atgataatgg tcgtgaggta tctgttcgct tttaggcaac
     2161 gaaagctcta tcccttcgtc gtccaacacc agtatttcct ttttccatat ctgataaact
     2221 tctccggtga taatttctcc aactttgtcc ttatattttc ggtaaatgtt atccttttcg
     2281 tattcctgca ctttgctgat cagattctgg cgaattgtaa gaatttcacg gcgtccgaaa
     2341 tcttcgagct tgatttcctg atacacctgt tctccaattt cgaaatccgg ctctaatttt
     2401 atggcctccg aatattcgat ttccttgttg ggatcggtaa gattcccatc ttcgactatg
     2461 gtcagagtcc tccatatttc aaggtctccc ttgtcgatat ttacaatgat gtcgaaattc
     2521 tcgtcagtac cgaatttttt aatcaaagta tgacggaaaa catcttccag gatgcgcata
     2581 agcgtttctc ggtcgatatt cttataatct ttgaactctg taaaagaggt tattaaatta
     2641 tgttccataa actcaaaatt ttatttcgtt gtaaagttat ttaaagctta gttcgatgcg
     2701 ggcttcttcg atattttcga aagggaaaaa tttctcgatg aaatggattt ttttctttcc
     2761 tttttcggtg atgatggttt cttgaatgtt caggacaatg ccctccggtt caactctttg
     2821 aagttctcct ctaacttgat gtttgtcggt caagatgatt ttgaattttc ggccgatgtg
     2881 tcgtttgtat tgccgtttaa ggcgaatggg tgcatcggcg cctgtcgacg aaacttccaa
     2941 agagtagtct tcttcgtcac ggtttagttt cgattcgata aaccgactta gtgtggcaca
     3001 atcctcaata tttatgccat gatcaccatc gataaataca aggattttat tccccggttt
     3061 aattttcaaa tcaaccagga acttgtcgct cccttcgagg tacaaatcaa tgatttccaa
     3121 tattttgaaa ctgtcaatca cttgtgaatt attattttag aaataaaaat tggggattga
     3181 atccccacct ttattttgca ttttccatgc gcataaatcg tttgcaaaat tagtatattt
     3241 taaaattaat caaaattatt agtatagaat gacatgaaat ttttgcaagt ataaattctt
     3301 attaaacagt gaataattaa acggatattg atagattgag taagtttgcc atgaaaattg
     3361 acttgagaaa tgccaaggat tttagttgta gatgatgagc gtgccatccg caataccttg
     3421 agggagattc ttgaatatga gaaatatatg gtggatgaag cgcaaaatgg acaggaagcc
     3481 attgaaaagg ttcaatccaa cgattatgat ttggttttgt gcgatattaa gatgcccggg
     3541 atagatggca tagaggtatt ggataagata ttagaacaag gtgaaacgcc tgttatcatg
     3601 atatcgggtc atggcacaat cgatactgca gtagaagcga ttaagaaagg tgccttcgat
     3661 tatatcagca aacctctcga tttgaatcga ttgcttgtca gtgtaaggaa tgcgttggat
     3721 cgctcgagat tgctcaccga aactcgaaat ctcaggaaga gggttttaaa gaataatcaa
     3781 atcataggcg aatcgaatgc cataaagaat gttctggcca tgttagagag agtagctcct
     3841 accaatgcta gagtattaat tacgggagaa aacggtactg gtaaagaact tgtagccagg
     3901 gcattacacg aaaatagtcc cagagctcat aacccatttg tggaggtgaa ttgtgctgct
     3961 attccttctg aacttatcga aagccaatta tttggacatg aaaagggatc tttcacttcg
     4021 gccatcaagc aaaggaaagg agatttcgaa ctggcaaatg gaggaacttt gtttctggat
     4081 gaaataggcg atatgagctt gtcggctcag gccaaggtgt tgaggtcgct acaggaaaat
     4141 aaaattacac gtgtaggagg agaaaaggaa attccggtag atgtacgaat aatagcagct
     4201 accaataagg atttgaaagc ggaaattgct aagggaaact ttagagaaga tctttatcac
     4261 cgtttgagtg tcatcatcat tcatgtacct tcgttagccg aacgaacaga ggatattcct
     4321 ttgctagcaa agcattttat ggaaattatt tcggccgaaa tgggtaaacc acccctcgaa
     4381 tttacccctg attccctcga agctcttaaa aaacggaaat ggactgggaa tgtgcgtgaa
     4441 ttgagaaata tggtagaaag gctggctata ttgtgcgatc gtgtgattac ggctgaagat
     4501 gtaataaaat attccaatta gagatcatcg tttgccttat ttctcagcat aggaacaaag
     4561 gcaaaaaaac catgttcttc tttcgtaaga cttccatcgg cattcttgat aagcaatgtc
     4621 atgatttgca tggaattttt acctatgggt accactaacc ttcctccagg cttcaactgc
     4681 tcgataagtg cctggggtat ttcgggagct ccagccgtaa taattatctt gtcgaatgga
     4741 gcataagcag gtaagccttt atatccatcg ccataaaaac accgtatatc agaaaatcct
     4801 gccgcttgca agaactcctt ggtttgttga taaagtttct tttggcgttc gatagtaaaa
     4861 acttttgctc caagtttcga taaaacgcag gcttggtatc cagaacccgt cccaatctcc
     4921 agaacctttt ccatgggctg tatatcgaga agttgagttt gaaatgccac tgtataaggt
     4981 tgcgatatgg tttgctggta tccaatcgga aagggtttat cctgataggc aaattcgaga
     5041 aatgccacat cgagaaacca atgacgtgga atttcttcga tagcttttaa tacattttca
     5101 ctggtaatgc cttttcttct gatttcttcg accagttttt tcctcaaacc tttatgacga
     5161 taattatctt cgaattttcg ctggagcata ttttactttg ttcgcactgc gaaattacgt
     5221 ttttagaatg aatttaagca ttccttattc gttaattctt gggctatttt ggagatgaga
     5281 tgatttttcc tacgtttgca ggttttatct tcgatagggg aaataattca tgaataagtt
     5341 gaaaataggt gttttaggtg caggtcatct ggggaagatt catattcgtt gcatacgaga
     5401 aatagaaact tatcggttga tgggttttta cgatccaga
//
LOCUS       DAORPD010000014         5332 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_2787_length_5332_cov_2.727876, whole genome shotgun sequence.
ACCESSION   DAORPD010000014 DAORPD010000000
VERSION     DAORPD010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5332
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2787_length_5332_cov_2.727876"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(162..1313)
                     /locus_tag="PK976_00350"
     CDS             complement(162..1313)
                     /locus_tag="PK976_00350"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007214906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HRC66370.1"
                     /translation="MNPKHLAILGCTGSIGRQCLDVVREHPQSFVVEILAAQGFSKAL
                     VSQAKEFRPKVVAVAKSSDFEVLRQAFEQDHIDVAAGENALAEVVAMPEIDMVVSAIV
                     GFAGLRSVIAAIEAKKHIALANKETLVVAGEWVMNRLKSLQVDLLPIDSEHSAIFQCL
                     EGEKPNKVEKIILTGSGGPFRGMSLERLQNVKPEDALHHPTWNMGPKISVDSATLMNK
                     GLEVIEARWLFHLNPNQIEVIIHPQSVIHSMVQFVDGSIKAQMSIPDMRLPIQYALSY
                     PERLPSSFQRFDFIRNSPLTFEAPNTHVFRNLILAFEALHQGGNMPCILNAANEIAVE
                     AFLQHRIPFLRMPVIIEKCMNMIDFISSPDLSQLIETDRITREVAQGFL"
     gene            complement(1313..2194)
                     /locus_tag="PK976_00355"
     CDS             complement(1313..2194)
                     /locus_tag="PK976_00355"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRC66371.1"
                     /translation="MHINVKNIRTVLFRNIKSKYRLVIQDAETFEEKFSFKLSRLNVF
                     ITLGILIIGLIFLTTYLIAFTSLKEYIPGYSNPDLSRQVYELTLRADSLELLLDQRAT
                     YLENIQRVLTGNVPLRKAADSTIQKHDYSKIDDTRIPEDSILRKEVENQDKYGLKPGY
                     STSIIKSSYISSFNFFRPVNGYLTSHFDPGHGHFGVDIATAEGETVKAILDGTVIQAG
                     WNIETGYTITIQHISNVVSIYMHNSSLLRKQGDIVKAGEPIAIAGSTGVLSTGTHLHF
                     ELWYNGTAIDPKNYIAF"
     gene            2795..>5332
                     /locus_tag="PK976_00360"
     CDS             2795..>5332
                     /locus_tag="PK976_00360"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRC66372.1"
                     /translation="MKSKNFIPKGIIIFLWIVTIIIPTGCSNKKNTFSRRVYHNLTAH
                     YNTWWNGNESLKEAIKDLEKNAKDNYTEVLPVYKLGSKKEATAINSKADRAIEKASKV
                     IQNNSMYFNKKEYNRWIDDSYMMIGKAYFYKQDYASARRTFEFILTRYKDSPLIPEIY
                     LWLGLTNNQMEQFSRSEAELEKFRMLSARKTMPRSMMLFYNKVLADFYLKQNRTEEAI
                     KTLHIATDLSRKKSDKTRLHFILGQLYMGKNDLIKAFQMFQEVIKRTPPYDMQFAAQL
                     NLARCFDVASGEAEKLEKMLQNMLKEEKNKFYQDQIYYALAEIALKKGDRTAAIENLR
                     LSVAKSKDNDFQRVQSSLTLADLYFEKPDYFCSQAYYDTALQALPKDHPTYEALQKKS
                     QVLTELVSNLTVVQTEDSLQRLAQIPENERLKIIDGIIAKYAEEEKKKQEEEMLRQSD
                     LTMLGQGNIQRQMDPSGAWYFYNPSAISLGFTEFKRKWGNRKLEDLWRLSNKQITTAW
                     EEEQTMAFSDSVANDSLLVKISTDPLKRQTYLQNLPLTPEKLRQSNQNIIEALFNAAF
                     IYREGLLDMVNAEKTFEELVERFPDTSLNKHYLLGCYQLVVINEKIENQSKADHYRNI
                     LITNYPTTDYARILGDPGYAHEIERKNNIVNEFYSNTYQAYRSGQYYLVVMNCDEAAS
                     RFPNHPLLPKFEFLKSLAIGKIDVIDSLAVNLQRFIMHYPKHELAAHARNILDKLSKL
                     DPSITSRIQLSGDSISLGTSAEPPKTPYLVETQSDHFVLLMVNPLQTDINALKIRIAD
                     FNERAFRLENFYTSDILLNDTLQIISIGVFKNASKAMDYYHHILDNEY"
BASE COUNT         1657 a         1076 c         1055 g         1544 t
ORIGIN      
        1 ttgaaagcgc gggccgcaag aaaatgacct aactcatgaa ggatcactaa aatactgaga
       61 cctaaaatga gctgaccggt catgataaga atattcataa aattttgtgg tattgatttt
      121 taatttatta cattcaatta acaaacatcg agcctaatgg tttataaaaa tccttgagct
      181 acttctctgg ttattctgtc cgtttcgatg agttgcgaaa gatcgggcga cgaaataaaa
      241 tctatcatgt tcatacattt ttcgatgatt actggcattc gcagaaaagg gatgcgatgt
      301 tgaaggaaag cttcaacggc tatttcgttg gcagcattga gaatgcaagg catatttcct
      361 ccctgatgca aggcttcgaa ggcgagtatt agatttctga aaacatgggt attgggtgct
      421 tcgaaagtga gtggagaatt tctgataaag tcgaaacgct gaaatgacga gggtagccgt
      481 tcagggtagg atagagcgta ctgaatgggc aaacgcatat cgggtatgct catctgagcc
      541 tttattgaac catccacaaa ttgtaccata gaatggatta cgctttgggg atgaatgatt
      601 acttcgattt ggtttggatt cagatgaaac agccatctgg cttcgattac ttcaaggcct
      661 ttattcatca atgtagccga atcgaccgaa attttaggac ccatgttcca tgtgggatgg
      721 tgtaaggcat cttcgggttt cacattttgc aacctttcga gactcatccc tctgaatggg
      781 ccaccagaac ccgtaaggat gattttttca accttattcg gtttttcgcc ctcgagacat
      841 tgaaaaatag ccgaatgttc agaatcgata gggagaagat ctacctggag agattttagg
      901 cggttcataa cccattcgcc tgccaccaca agcgtttcct tattggccaa ggcaatatgt
      961 ttttttgctt ctatggctgc tatcaccgac cgaaggcctg caaaaccgac gatagctgat
     1021 actaccatgt caatttcggg cattgcaaca acttctgcca gcgcattttc accggcagcc
     1081 acatcgatgt gatcctgttc gaaggcctgt cgcagcactt caaaatcact tgatttggca
     1141 acagcaacca ctttgggtct gaattccttt gcctgcgata ccagagcctt cgaaaatcct
     1201 tgagcagcca atatttccac cacaaaactc tgaggatgtt cccgaactac atcgaggcat
     1261 tgacgaccaa tgcttcctgt acaaccaagt atagccaagt gtttgggatt cattagaatg
     1321 ctatgtaatt tttagggtca attgccgtac cattatacca gagctcgaaa tgaagatgtg
     1381 tccccgtgct gagtacacct gtagaacccg caattgcaat gggctcaccc gccttgacaa
     1441 tatcaccttg cttgcgcagt aaagatgaat tgtgcatata aatagaaact acattcgata
     1501 tatgttgtat agtaatggta tatccagtct ctatattcca tcctgcctga atgactgtgc
     1561 catcgagtat agcttttact gtttcaccct cggcagttgc aatatctaca ccaaaatgcc
     1621 catgccccgg atcaaaatgt gaagttagat aaccatttac aggtctgaaa aaattgaacg
     1681 acgaaatata tgaactcttt attatagatg ttgaatatcc aggcttcaac ccatacttgt
     1741 cctgattttc cacctccttg cgcaatattg aatcctcagg tattctggta tcgtctatct
     1801 tcgaataatc gtgcttttga atagtgctgt cggcagcctt acgtaaagga acattacccg
     1861 taagcactct ttgaatattt tccaggtaag ttgctctttg atcgagtaaa agttcgagtg
     1921 aatcagcccg taaagtaagt tcgtaaacct gacgactaag atcgggattg ctgtatccag
     1981 gaatatattc tttgagagaa gtaaaggcaa ttagataggt ggtgagaaaa ataagaccaa
     2041 tgataagaat acccaaagta ataaacacat tgaggcgcga tagcttgaag ctgaatttct
     2101 cctcgaaggt ttcggcatcc tgaataacta atctatactt tgatttaata ttccggaaaa
     2161 gaacagtcct tatgttttta acattgatat gcataattca atctgctacc aggaatattc
     2221 cctgaatcct caaagccatc agttttaaag caatgaataa cttaattcta tctgtaaata
     2281 cttgtatgag aatgcaagtg atttaattgg ccgcaaattt aatgataaac actctacgtt
     2341 ccattttgtg atttatcagc atgaattccc ccaatcgaca cggaaggcaa actggaattc
     2401 tgaagcattg aatttcttat cgatgatgtt attattggtg ataacctact tctgttatgt
     2461 acaatgtttt ttatcaatca gataacattg taattcgtcc agaaaactag caagctaatt
     2521 ctcattgccg ataatttagc cttcaacgat ggtttatcga tctttcggta gatatcacgg
     2581 atggttttta ctgttttaac agtcgacttt gagggttttc ccttttggtt agtaaagaga
     2641 ggtagcggtt ttacaataaa gaatcaccat ttatcggcca aggaggtaaa attgagagtt
     2701 aaagtggcaa tgtttcacca aaccaaggaa atgaaataat tttgcatctt cgaagttatt
     2761 caatatacgt taatttccag aaaaaccgat tattttgaaa agtaaaaact tcatacccaa
     2821 aggtataatc atttttcttt ggattgttac aattattatt cctacgggct gttcgaataa
     2881 gaaaaacacg tttagccgca gagtttatca caatcttacg gctcattaca atacgtggtg
     2941 gaatgggaat gagagtttga aagaagccat caaggatctg gaaaagaatg caaaggataa
     3001 ttataccgag gtgcttcccg tttataagtt gggatcaaag aaggaggcaa ctgctattaa
     3061 ttcgaaagcc gatcgagcca tcgaaaaggc ttcaaaggtt atacagaata acagcatgta
     3121 tttcaataaa aaagaatata accgatggat agacgacagt tatatgatga tagggaaggc
     3181 atatttttac aagcaggatt atgcctcagc tcgaaggact ttcgaattta tcctcacccg
     3241 ttataaagat tctcctctga ttccagaaat ttatctttgg ttaggattaa ccaacaatca
     3301 aatggagcaa ttttcgcgct ctgaagctga attggagaaa tttcgtatgc tttcggctcg
     3361 aaaaaccatg ccacgcagta tgatgttatt ctacaacaaa gttttagccg atttctacct
     3421 aaaacagaat agaacagagg aggccatcaa aacccttcat attgccacgg atttaagccg
     3481 aaaaaaatct gacaagacac gtcttcattt cattttggga cagttgtata tgggaaagaa
     3541 cgatcttatc aaggcctttc aaatgtttca ggaagtcatt aaacggactc ctccttacga
     3601 catgcagttt gcggcacagc tcaatcttgc ccgctgtttc gatgtagctt cgggtgaagc
     3661 cgaaaagctc gaaaagatgc ttcaaaacat gttgaaggaa gagaaaaata aattttatca
     3721 agaccaaatt tattatgctc tggctgagat agccttaaaa aagggagacc gtactgcagc
     3781 aatcgaaaat cttcgccttt cagtagctaa gagtaaagat aatgattttc aaagggttca
     3841 atcctcattg actttagccg acctgtattt cgagaagcct gattattttt gctcacaagc
     3901 ttattatgac actgctttgc aggccttgcc caaagatcat cccacttatg aggctttgca
     3961 aaagaaatct caggttctta ccgaattagt aagcaacctt acagtagtac aaaccgaaga
     4021 tagcctccag cgtttggctc aaatacccga aaatgaacgt ttgaaaatca tcgatggcat
     4081 tattgcaaag tatgccgaag aagaaaagaa aaaacaagaa gaagaaatgc ttcgccagag
     4141 cgaccttacc atgctggggc aaggcaatat tcagcgccag atggaccctt cgggcgcctg
     4201 gtatttttat aacccttcgg ctataagcct cggttttact gaattcaaaa ggaaatgggg
     4261 caaccgaaag ctcgaagacc tttggagact cagcaacaaa caaataacca ctgcgtggga
     4321 agaagaacaa actatggcct tttccgattc agtggctaat gacagcctgt tggtaaagat
     4381 ttctaccgat cccttgaagc gccaaactta tttgcaaaac cttccattaa cacccgaaaa
     4441 acttcgtcaa agcaaccaga atattatcga agcgcttttc aacgcagcct tcatttatcg
     4501 cgaaggattg ctcgatatgg taaatgccga aaaaaccttc gaggaattgg tagaacgttt
     4561 ccctgatacc agtttaaaca aacattattt gcttggttgt taccaattgg tagtgattaa
     4621 cgaaaagatt gagaatcagt caaaagccga tcattaccgt aatattctga ttacgaacta
     4681 tcctactacc gattatgcca gaattttagg cgacccgggt tatgctcatg agattgaaag
     4741 gaaaaataat atcgtcaacg aattttattc gaatacttat caagcttatc gatcgggcca
     4801 atattatctg gtagtaatga attgcgatga ggcagccagc aggtttccca atcatcctct
     4861 tttgccgaaa ttcgaatttt taaagtcatt agctattgga aaaattgacg taatcgattc
     4921 gttggcggtc aatctgcaac gttttatcat gcattatcct aaacacgaac ttgctgctca
     4981 tgctcgcaac attctcgata aactctccaa attggaccct tcgattacct caagaattca
     5041 actatccggt gatagtatat ctctgggtac atctgccgaa cctccaaaaa cgccttatct
     5101 ggtcgaaaca caaagcgatc attttgtact gcttatggtt aatcctctac aaaccgatat
     5161 caatgcattg aaaatcagga ttgccgattt taacgaacga gcctttcgcc tcgaaaattt
     5221 ttatacttca gatattttgc ttaacgacac tttgcagatc atcagcattg gtgtctttaa
     5281 gaatgcttcg aaggccatgg attattatca ccacattctg gataatgagt at
//
LOCUS       DAORPD010000015         5127 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_2898_length_5127_cov_2.101735, whole genome shotgun sequence.
ACCESSION   DAORPD010000015 DAORPD010000000
VERSION     DAORPD010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5127
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2898_length_5127_cov_2.101735"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..851
                     /locus_tag="PK976_00365"
     CDS             <1..851
                     /locus_tag="PK976_00365"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC66373.1"
                     /translation="KWNDGNWTLTDIGGVAKIWNSSNKSYEDVNSNGIIPATQGFFVQ
                     VSASTSLTIPAASRVHSSTNWYKSSDALSIVLAASPADNSSTQKTYIRTEEGTTEGFE
                     FSSDSRFLAGYAPKFYCMVGGEKLSTQSLPTIYNGLELNYGFEKLENVNQYRIQLIEN
                     PYSATLYLKDLKTGSIQDMSQNPVYEFTASEGDPALRFKLSFGSVGIDEPINNNYRLY
                     YSNGIMYMLQMDGHKQIEVYQLNGQLIRKLNSTENFVNLQLAKGIYLVRVINANQAYV
                     QKMIVQ"
     gene            871..1107
                     /locus_tag="PK976_00370"
     CDS             871..1107
                     /locus_tag="PK976_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66374.1"
                     /translation="MKRYIFRLMLFAAILVASLSVYSQPQNPPPPPAGGHGQSTNQSR
                     GGGAPLGTGIGIMLALGGAYGAKKVYDARKNSKE"
     gene            complement(1778..3034)
                     /locus_tag="PK976_00375"
     CDS             complement(1778..3034)
                     /locus_tag="PK976_00375"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRC66375.1"
                     /translation="MISKLRIINDPVYGFIPIPNELIFSLIEHSFFQRMRRIKQLGLT
                     HLVYPGALHTRFHHSLGSMYLMREAIQVLRERGIEISEQESEGALIAILLHDLGHGPF
                     SHSLESAIVRNIGHEHLTGLFLNELNKEYDDALGIGLSMFQGDYERKFFHQLLSGQLD
                     MDRLDYLRRDSFFTGVSEGQIGSERIIKMLNVVDDNLVIEEKGRLSLEHFLSARRIMY
                     WQVYFHKTVLAAETLLIKILNRAKYLAAQGEKLFATTHFLRFLREDFFLCDFQEDPTL
                     LRSFARIDDFDVFSSIKAWTEHPDPILSCLSNYLVDRRLFRIYFSDKPFDHNHLEIAK
                     NRVEKILNLKKGEGDWFIGTGEVCVMAYDQETGPILVKTAQGNIEELISAISSPNNLI
                     PLQPEVKYFLCLPKTVKLNPDSEEEE"
     gene            3290..4255
                     /locus_tag="PK976_00380"
     CDS             3290..4255
                     /locus_tag="PK976_00380"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRC66376.1"
                     /translation="MKSLLKIFVCAIMAIMVSSCVSQKKYTETEQKRRQCEEELIAQK
                     SQNNDLIADNTELKVRLERLNKNFQRLINDTIRIGQNLRDFQADYERLKFSYNELLDI
                     AGKTEAGNKAEIQRIMAELQSSQEKLIHKQDSLQALEAELAVKQKHLNELQKILNQKD
                     SIVNALRQKVSQALLGFEGKGLSIEIRNGKVYVSLEESLLFRSGSWEVNDRGISALQK
                     LAEVLASNPDINVLIEGHTDNVPYRGSGQVKDNWDLSVMRATAIVKVITRNPGVHPSR
                     LTAAGRSEYAPLATNSTSEGRAKNRRTEIILTPKLDELFQIIETH"
     gene            complement(4478..>5127)
                     /locus_tag="PK976_00385"
     CDS             complement(4478..>5127)
                     /locus_tag="PK976_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HRC66377.1"
                     /translation="VKVELRMIHSEFLTQENLSEKLSGLHGILVAPGFGIRGIEGKIL
                     AIHYARTRGIPFLGICLGMQCAVIEFAQNVMGLKKANSTEFDPRTPDPVIDIMEQQKK
                     ISGMGGTMRLGAYPCQLMEGSKSRNIYKVPLILERHRHRYEFNNKYLEQFEQAGMIAA
                     GINPKENLVEIVEIPKHPWFIGVQFHPEFKSTVASPHPLFISFVEAACLHLEQNT"
BASE COUNT         1594 a         1063 c          953 g         1517 t
ORIGIN      
        1 taaaatggaa cgatgggaat tggacactta cagatattgg tggagtagca aaaatttgga
       61 attctagcaa taaatcttat gaagatgtca attcaaatgg cataatacct gccacccaag
      121 gattttttgt acaggtaagt gcttcaactt ctctcactat accagcagct tcacgagttc
      181 acagttcaac caactggtac aaatcatcgg atgctttgag tattgtttta gccgcttcgc
      241 cagccgataa ttcttcaacc caaaaaacct atataagaac agaagaagga actactgaag
      301 gtttcgaatt ttcatccgac agccgtttct tggcgggtta tgcaccgaag ttttattgca
      361 tggttggagg cgaaaagctt tctacgcaat ccttacccac aatctataat ggtctcgagc
      421 taaattatgg ttttgagaaa ttggaaaatg taaatcaata tcgtatccaa ctcatcgaaa
      481 atccttattc tgccacactt tacctgaaag atttaaaaac tggaagcata caagatatga
      541 gtcaaaaccc ggtttatgaa tttacagctt cagaaggcga tcctgcgctg cgtttcaaat
      601 taagctttgg aagcgtaggt atagatgaac cgattaataa taattaccgg ctctactatt
      661 ccaatggtat aatgtatatg ctgcaaatgg acggacataa acaaatcgag gtttatcaac
      721 tcaatgggca attgataaga aaactcaatt ctaccgaaaa ttttgtcaat cttcagcttg
      781 ccaaaggaat ctatctggta agagtaatta atgccaatca ggcttatgta caaaaaatga
      841 ttgttcaata atatataaat ctataaactt atgaaaagat acatttttcg attgatgtta
      901 ttcgcagcta ttttagttgc ttccctttcg gtttattctc aaccacaaaa tcctcctcct
      961 cctcctgcag gtggtcatgg acaatctact aatcagtcaa gaggtggtgg tgctccttta
     1021 gggacgggta ttggtataat gcttgcctta gggggtgcct atggagctaa aaaagtttat
     1081 gacgctcgca aaaattcaaa ggagtaaacc ttacataaat ctcttttcat ggctaaagag
     1141 gctgctctca tcttgggagc gacctctttc gtctttatga tcatgattgt ataagaatct
     1201 tatttttatt tcagatttat tttagaattc ctgattcaaa aatagatcgg tttatcgtgt
     1261 ttatgcaaac aagcgcatag ccttcacaaa actgaatact taagtgcatt gagttgttga
     1321 ggtgaactcc ttttgtcaga atctcttgat gctgttttaa aatttccctt tttgataaga
     1381 taataaatgg cagcctcacc aatgccaggt tctttcgctg tgctgtatta cgaacactcc
     1441 ttattcatcc tcgcctgtgc ttcccgcttc atcgattctg taaacttgta acactgccca
     1501 cgatgctttg cgcgattaac aaacccagtc tgcacctttc tctttcagtg atttgacaca
     1561 actctgaaca ttccttacgt gaatacccag ggggctgttt aagaaaaggc aatcgaagtc
     1621 gtacgtccga aaatttttca tcggggaaat gcccattcga atcatcatca tatttttaaa
     1681 aatattgaaa ataaaaataa agagtcgaaa gtttcaaatt tatctccctg cctatatatt
     1741 tcttcctcgc cttgaatctc ttccattcaa aaaatcttca ttcttcttct tcagaatcag
     1801 ggttcaattt cactgtttta ggcaagcata aaaaatactt tacttcgggt tgaagaggta
     1861 ttaaattatt gggtgatgaa atcgcagaaa ttaattcctc gatatttccc tgtgctgttt
     1921 ttaccaaaat aggcccagtt tcctgatcat aagccatgac acatacctca cctgttccta
     1981 taaaccaatc tccttctcct tttttgagat tcaatatttt ttccactcta tttttagcaa
     2041 tctctaaatg attatgatcg aagggtttat ccgaaaaata aattctaaaa aggcggcggt
     2101 cgaccagata attgctcaaa caggataaga tagggtcggg gtgctcagtc caggctttta
     2161 tcgaggaaaa cacatcgaaa tcatcgatgc gtgcaaaaga ccttagcagt gtaggatctt
     2221 cctggaaatc acataggaag aaatcttctc gaagaaatcg taagaaatgc gttgtagcaa
     2281 acagtttctc gccctgagct gctaaatact tggctctatt taaaatttta atgagtaggg
     2341 tttcggcagc aagcacggtt ttgtgaaaat atacctgcca atacataata cgccttgccg
     2401 aaagaaaatg ctcgagcgac aaccggcctt tttcttcgat taccaggtta tcatccacta
     2461 catttagcat tttaataata cgttccgagc caatttgacc ttccgatact cccgtaaaaa
     2521 aactatccct tcgaagataa tccagacgat ccatatcgag ttgccccgat aagagctgat
     2581 ggaaaaattt cctctcataa tcgccttgaa acatggataa acctattccc aatgcatcat
     2641 catattcttt attcagctcg ttcaggaaaa ggcctgtcaa atgttcatga cctatattcc
     2701 taacaattgc actctccaat gaatgcgaaa atgggccatg acccaaatcg tgcaacagaa
     2761 ttgctatcaa tgctccttcg ctttcctgct cgctgatttc tatcccacgc tcacgtaaaa
     2821 cctgaatggc ttccctcatc agatacatcg aacccagtga atgatgaaaa cgcgtatgca
     2881 gcgctcctgg ataaaccaaa tgcgtaagcc ccaactgttt tatccttctc atacgctgaa
     2941 aaaacgaatg ctcgatcagg ctaaaaatta gctcgttagg tatagggata aacccataaa
     3001 ccgggtcatt gataattcta agcttactaa tcaatccctt tgctatttat ctttatgcaa
     3061 acataatcaa tcattctatt ttccaataat atcatcagaa tgataattga acgaaagcta
     3121 tttatttttg caagtatgaa acgaagatcc cgttctatat gataactgtc ttatatatac
     3181 tacaagatgg aatgggggtt atttaaatcg cgcagtcaaa aaatctctta taaagacgat
     3241 ttgtaatagt ttaacgttat ttttttgaat caaataaaat cgacaccaga tgaaaagttt
     3301 actgaaaatc ttcgtttgtg caataatggc catcatggtt agttcatgtg tttcccagaa
     3361 aaaatatacc gaaaccgaac aaaagcggcg ccaatgcgaa gaggagctta ttgcccaaaa
     3421 aagtcaaaac aatgatttga ttgccgataa taccgaactc aaagtacgcc tcgaaagact
     3481 aaataagaac tttcagcgtt taatcaacga cactatccgc atagggcaga atcttcgcga
     3541 ttttcaagct gattatgaaa gattgaaatt ttcgtacaac gaattgctcg atattgcggg
     3601 gaagacggaa gccggcaata aagccgagat tcagagaatt atggctgaat tgcagtcatc
     3661 tcaggaaaaa ttgattcata aacaagattc attacaagca cttgaagcag aattggctgt
     3721 taagcaaaag catttgaatg agttgcagaa gattctaaat caaaaagatt ccattgtcaa
     3781 tgcattgagg caaaaagtct cgcaagcttt gttaggtttc gagggcaaag gcttgagcat
     3841 cgaaattcgt aatggtaagg tttatgtttc gctggaagaa agcctgcttt tcaggagtgg
     3901 ttcatgggag gtaaacgaca gagggatttc tgctcttcaa aaactggctg aggttcttgc
     3961 ctctaatccc gatatcaatg ttcttattga aggccatacg gataatgttc cctatagagg
     4021 aagtggacag gtaaaggaca actgggatct cagcgtaatg agggcaacag ctatcgtaaa
     4081 agtcattact cgtaatcccg gagttcatcc cagccgtttg actgctgctg gaagaagcga
     4141 atatgcgcct cttgctacta acagcacaag cgaaggcaga gcaaaaaacc gccgaaccga
     4201 aatcattctt actccaaaac tcgacgaact ctttcagatt atcgaaacac actaacacat
     4261 gtggtcagga gttcaaggtt tctgatggat ggcgagaagg gattggtgag ataaaagtta
     4321 aaagttgaaa ggatgagatt gaatatatga gcattaaaaa atatttcata taatcttatt
     4381 cgagctgact ttccaattac ctgtcttaaa agttccctta tcttctcgtc ccctcgtacc
     4441 tcaggcaccg aatctcccaa tatcctgaca acacttatca agtgttttgt tcgaggtgca
     4501 gacaggcagc ttctacaaag cttataaata atggatgagg cgaagccact gtacttttaa
     4561 attctggatg aaattgaacg cctataaacc atggatgctt tgggatttcg acgatttcaa
     4621 ccaaattctc tttgggattg atgccggcgg ctatcattcc ggcttgttcg aactgttcga
     4681 gatatttatt attaaactcg taacgatgcc tatgtcgctc gaggataagt ggaaccttat
     4741 aaatatttct tgatttcgat ccttccatca actggcaggg ataagcccct aaccgcatgg
     4801 ttccacccat acccgaaatc tttttctgct gttccatgat gtcgatcaca gggtcaggtg
     4861 tacgtggatc gaactcggta gaattggcct tcttgagtcc cattacattt tgggcaaact
     4921 ctattacagc acattgcata cccagacata ttcccaaaaa cggaattcct cttgttcgag
     4981 cataatggat ggccagaatc ttaccctcga taccccgaat tccaaatcca ggtgcaacta
     5041 atataccatg aagccccgat agtttttccg aaagattttc ttgggtcaaa aattccgaat
     5101 gtatcattcg cagttcaacc ttaacct
//
LOCUS       DAORPD010000016         5001 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_2991_length_5001_cov_2.104933, whole genome shotgun sequence.
ACCESSION   DAORPD010000016 DAORPD010000000
VERSION     DAORPD010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5001
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2991_length_5001_cov_2.104933"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            8..81
                     /locus_tag="PK976_00390"
     tRNA            8..81
                     /locus_tag="PK976_00390"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42..44,aa:Arg,seq:tct)
     gene            391..900
                     /locus_tag="PK976_00395"
     CDS             391..900
                     /locus_tag="PK976_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HRC66378.1"
                     /translation="MGKEAIKILSVDRDELLKMLNAALAEEWLAYYQYWIGARLMEGP
                     MRAEIEPELLLHATQELNHAVLLVNRIIQLEGTPILNPNDWSKLAHCQYEEPKDPYIE
                     VILDQNLKGERCAIERYEQIANFTSGKDHATYQMAVQILNEELEHEHDIQDWINDIHR
                     MKEEWKKLR"
     gene            1133..1789
                     /locus_tag="PK976_00400"
     CDS             1133..1789
                     /locus_tag="PK976_00400"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HRC66379.1"
                     /translation="MDNKSTGIIWDWNGTLLDDVSLCIDVMNSLLNDRHLPLLNHERY
                     REIFDFPVIEYYKKLGFNFNVDPFEVIGLEFMKRFLEHIEKVPLMDGAVEILEWFDNK
                     GYRQYILSAMEQQSLDKILLDKGIHPYFEAISGINNHYGGGKMEEGRHFITDNKMNPN
                     EAYLFGDTIHDFEVGDRLGMKVVLIAQGHQSKQRLIETKAVVVDNFEQLKKWFEINGK
                     "
     gene            1843..2094
                     /locus_tag="PK976_00405"
     CDS             1843..2094
                     /locus_tag="PK976_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66380.1"
                     /translation="MKTTILGLKITQRAEKSTEVQDVLTKFGCSIHTRLGLHEASEDY
                     CSQEGLIILELVGDVGEMLKLEEALKQIPGVEVQKMVFD"
     gene            2097..2858
                     /gene="truA"
                     /locus_tag="PK976_00410"
     CDS             2097..2858
                     /gene="truA"
                     /locus_tag="PK976_00410"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HRC66381.1"
                     /translation="MDNQRYFIHLAFDGTNYCGWQRQKNATSIQQEIEEKISILLKKP
                     IDITGCGRTDTGVHASSFYAHFDFDPMPKQQLIILSYRLNHILPLDIAISEILPVKPE
                     AHARYDALWRTYRYYISLRKNPFTRNYRWFRSQCPDIEKMNQLAEILLAVKDFTSFSK
                     LHSDTENNLCTLTQAFWEIRKDELVFTITANRFLRNMVRAIVGTLWQLGKEENAVDHL
                     REIIAKRDRSAAGPSVNPHALFLEEIIYPDDIFLV"
     gene            complement(2855..4093)
                     /locus_tag="PK976_00415"
     CDS             complement(2855..4093)
                     /locus_tag="PK976_00415"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectonucleotide
                     pyrophosphatase/phosphodiesterase"
                     /protein_id="HRC66382.1"
                     /translation="MKLDRRIFFKAFSLLVALSIILGLSFGCNRARQADEKRYLVVLS
                     LDGFRWDYPQYFSTPNLDKMAQEGVHAHSLIPCFPTKTFPNHYSIATGLYPDHHGIIQ
                     NSFYVPAIGTYEPTNRQSIMNGAFYEGEPIWLTAENQGMKSACCYWVGSEAPIKGKYP
                     SIWLRYNQDLPFTSRIDSVIAWLQLPSEKRPHLIMWYMHQPDKIGHHEGPLAASTGRM
                     VSYLDSLVGVFRARLNELPIAKKVNFVVVSDHGMAKVNESNNLYVDQLVPRYWITMAC
                     GGEVQMNLDGIPAYKDSLYQRLKKVNHLTVWKKEEVPQRLHYSTNPRIYDLVVLADTG
                     WNIMLTHPAKILPGHHGYDNVYPDMHGIFYGVGPAFKNGYERPSFENIHLYPLFAHIL
                     KLKPAVVDGRLKEVEDILKD"
     gene            complement(4274..>5001)
                     /locus_tag="PK976_00420"
     CDS             complement(4274..>5001)
                     /locus_tag="PK976_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66383.1"
                     /translation="KSKNIHERVLSHLYNETTRKAVEMKHAVADISYEITGSDLIAQL
                     LESEEIKKHKPLYNRQQRRTGFRIGLFTYFDEKGYMRLKVDKLSDGLLPVTTFNSVMM
                     AKRALFALCDEYHLCQKLCGLYKTGGACFKHGIGQCMGACIGLESPADYNTRVEAAIA
                     PIKFEHENFFIIDKGRRSDEYSVVEVRHGAYLGFGYFDGTDFDGTADYLHSCIHRCID
                     TRDARHIILSGLKHHEYKRLLVY"
BASE COUNT         1596 a         1020 c         1016 g         1369 t
ORIGIN      
        1 ccaaaatggt ccggtagctc agttggatag agcagcagcc ttctaagctg cgggtcgggg
       61 gttcgaatcc cccccggatc actcccgatg aaacttatat ttgtccctct ccctcttatt
      121 tcaccgaaat cgttttatca attgcttaaa tggcaactcc tttatcaatt cgtaaaacct
      181 tatgagagcc tatcgagaat attacaatga aaatatttcg atatacgttt taaagggcct
      241 tattcgtaaa taaatttttt cagcctgttt catcaataga aaaattttta taaatttgta
      301 atcagaattt attggatcga aacgaggcga tataaaggaa actctggttt taccatatca
      361 aatagttaac aactaaaaaa cagatggatc atgggaaaag aagctattaa gattttatca
      421 gtcgaccggg atgagctcct gaagatgttg aatgccgcct tagcggaaga atggcttgcc
      481 tattatcaat actggattgg ggcaaggttg atggaagggc cgatgagggc cgaaattgaa
      541 cctgaactgc tgctgcatgc tacacaggaa ttaaatcacg ccgtattatt ggttaacaga
      601 attatccagc tggaaggaac acccattcta aatcccaacg actggagcaa gttagctcac
      661 tgccaatacg aagaacccaa agacccttac atagaggtta tactcgatca aaaccttaaa
      721 ggagaaaggt gcgctatcga gcgttacgaa caaatagcca actttaccag tggtaaggat
      781 catgcaacct accagatggc cgttcaaatc ctcaacgaag aactcgaaca cgaacacgac
      841 attcaggact ggattaacga cattcacaga atgaaagaag aatggaaaaa actccgataa
      901 accggagttc tttctataac atgctaaaaa acaccactaa actctactct caatttttga
      961 ggcatttact atcttcagct gccttatttc ccgaattctt ggggtggctg aagatttttc
     1021 aaaagaaccc tcttaaatca aaagacgaag gttcaggttg aaccggccaa ataaaacgtc
     1081 ttctattgca aaaataaaaa cctaattgtt gtaatcagtt aaagatacct cgatggacaa
     1141 caaatctacg ggcattatct gggattggaa tggcacattg ctcgatgatg tttcattatg
     1201 catcgatgta atgaattcat tgctaaatga ccgacatctt cctctgctaa atcatgaaag
     1261 atacagggag atttttgatt tccccgtaat agaatattat aagaaattag gattcaactt
     1321 caatgtggat cccttcgagg tgattggtct tgaatttatg aagaggtttt tagaacatat
     1381 agaaaaagtt ccgctgatgg atggtgctgt tgaaatactc gaatggttcg acaataaagg
     1441 ttaccggcag tacattcttt cggccatgga acaacaatcg ctcgataaaa tattgctcga
     1501 caaaggtatt catccttatt tcgaagctat ctcaggtatt aataatcact atggcggagg
     1561 aaaaatggaa gaaggcagac attttatcac cgacaacaaa atgaacccca acgaagctta
     1621 cctgtttggc gacactatcc atgattttga agttggagat cgtttgggga tgaaggttgt
     1681 gctaatagct cagggtcatc agtcgaaaca acgtcttatc gaaacaaaag ctgttgtggt
     1741 ggataatttc gaacaactca agaaatggtt cgaaattaac ggtaaatgat gtaaaaatta
     1801 agaaaaagga attaaatttg ccaaaaacca aaaaatattc ttatgaaaac gacgatttta
     1861 ggacttaaaa ttacgcagag agccgaaaaa tcaaccgaag tacaggatgt tttgaccaaa
     1921 tttggttgtt ctattcatac gcgactggga ttacacgaag caagcgaaga ttattgcagc
     1981 caggaaggcc ttatcatttt ggaactggtt ggcgacgtag gagagatgct gaaattagaa
     2041 gaagcgctca agcaaattcc aggcgttgaa gttcaaaaga tggtgttcga ttaagggtgg
     2101 acaaccaaag atattttatt catcttgcct ttgatggcac caattattgt ggatggcaac
     2161 gccagaaaaa tgctaccagc atccagcagg agatagaaga aaaaattagc attcttttaa
     2221 agaaacctat tgacattaca ggttgtgggc gcaccgatac aggggtgcat gcttcctctt
     2281 tttatgccca tttcgatttc gatcctatgc caaaacaaca gctgataatt ctttcctatc
     2341 gactcaacca cattctaccc ctcgatatcg ccatttcaga aatcctgcct gtaaaacctg
     2401 aagcgcatgc ccgatatgat gcgctttgga gaacttaccg atattacatt tccctgcgaa
     2461 aaaacccatt tacaagaaat tatcgctggt ttcgatcaca atgtccagat atcgaaaaaa
     2521 tgaatcaact ggctgaaatt cttttggcag tcaaagattt tacgagtttt tccaaacttc
     2581 attcggatac cgaaaataac ctttgcacgc ttactcaggc tttttgggaa atcagaaagg
     2641 atgagcttgt ttttaccatc acagccaacc gtttcttacg caacatggta agagccattg
     2701 taggcacgct ctggcaattg ggaaaagaag aaaatgccgt tgatcacctt cgtgaaatta
     2761 tagccaaaag agatcgttcg gctgccgggc cttctgtaaa ccctcatgct cttttcctcg
     2821 aagaaatcat ttatcccgat gatatcttcc ttgtttaatc tttcaatata tcctctactt
     2881 ctttgagccg accatctact actgcaggtt ttagtttcaa aatatgagca aataagggat
     2941 acagatgaat attctcgaaa cttggccttt cgtaaccatt tttgaaagca ggccccactc
     3001 cgtaaaaaat tccgtgcata tcaggataaa cattgtcgta accatgatgg ccaggaagaa
     3061 tttttgccgg gtgagtaagc atgatgttcc aacctgtatc cgctaaaaca actaaatcat
     3121 aaatacgggg attcgtacta taatgcaaac gctgaggcac ttcttccttt ttccaaaccg
     3181 tgagatggtt tacttttttt aaacgttgat ataaagaatc cttataggcc ggaataccat
     3241 ccagattcat ttgaacttcc ccaccacagg ccatggtgat ccagtaacga ggcacaagtt
     3301 ggtccacata caggttattg ctttcgttaa cttttgccat tccatggtcg gaaaccacaa
     3361 caaagtttac tttctttgct atgggcaatt cgttgagtcg agcacgaaaa acgcccacca
     3421 gtgaatcgag ataggaaacc attctgccgg tcgaagccgc aagcggtcct tcatgatggc
     3481 caattttatc gggttggtgc atataccaca tgatcagatg aggtcgtttt tcggaaggca
     3541 attgcaacca tgcaataact gaatcgatgc gggaggtaaa aggcaaatcc tgattgtagc
     3601 gaagccaaat agatgggtat ttccccttga tgggagcttc actgcctacc cagtaacaac
     3661 aagccgactt catgccttga ttttctgctg taagccagat gggctcgcct tcataaaatg
     3721 ctccattcat aatgctctgc cggttagttg gttcataagt cccaatcgca ggtacataaa
     3781 atgaattttg tataattccg tgatggtcgg gatataagcc cgtggcaatg gaataatggt
     3841 tggggaaagt cttggtaggg aaacaaggaa taagagaatg cgcatgcacc ccttcttggg
     3901 ccatcttatc gagattggga gtagaaaaat actgaggata atcccatcgg aaaccatcca
     3961 gtgaaagaac caccagataa cgtttctcat cggcttgccg cgccctgttg cagccgaaag
     4021 ataaccctaa aattatagat agtgcaacaa gcaggctaaa cgctttaaaa aaaatacgcc
     4081 ggtcgagttt catgatttct atgtttttcc gaacaaaaat aagaagagca tctcaaacat
     4141 aagttaattc aatttaaaga aataattaca tgtttgaagc ctttgaattc aaatagctga
     4201 taaactgtaa aatataaaaa atagtcgatt atcgaaaatg aggtaaaaaa gttttatgaa
     4261 ttgaatcaga tgctcaataa accaacaaac gtttatattc atgatgttta agaccggata
     4321 ggatgatatg tcgagcatcg cgggtatcga tgcaacgatg aatgcaggaa tggaggtaat
     4381 cggctgttcc atcgaaatcg gttccatcaa aatagccaaa acccagataa gcaccatgcc
     4441 taacttctac cacggaatat tcatcggagc ggcgcccctt atcgatgata aaaaaatttt
     4501 catgttcgaa tttaatagga gcaatggccg cttctacacg agtgttgtag tcggcagggg
     4561 attccaatcc tatgcaggct cccatgcatt gaccaatgcc atgcttaaaa caggcacccc
     4621 cagttttata caaaccacaa agcttctgac acagatgata ttcatcgcat aaagcaaaga
     4681 gagcacgctt ggccatcata accgagttga aggtagtaac aggaagtaaa ccatcggata
     4741 atttatctac tttcagtcgc atatagccct tttcgtcgaa ataggtaaaa agtcctatac
     4801 ggaaacctgt ccgtcgctgc tgacggttat acaagggttt atgttttttt atttcctccg
     4861 attcgagtaa ctgtgcaatg agatcgctgc ctgtaatttc gtaagaaata tcggcaacgg
     4921 catgtttcat ttcaacagcc ttgcgtgtag tttcattata gaggtgcgac agcacccgct
     4981 catgaatgtt tttacttttg c
//
LOCUS       DAORPD010000017         4937 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_3039_length_4937_cov_2.405162, whole genome shotgun sequence.
ACCESSION   DAORPD010000017 DAORPD010000000
VERSION     DAORPD010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4937
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3039_length_4937_cov_2.405162"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="PK976_00425"
     CDS             <1..208
                     /locus_tag="PK976_00425"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lauroyl acyltransferase"
                     /protein_id="HRC66384.1"
                     /translation="FRIPVYYFGIRRLERGRYEVFAEQISSNPHQSEFGEITRNYVRI
                     LEQEILNHPDNWLWSHRRWKNLAE"
     gene            complement(221..775)
                     /locus_tag="PK976_00430"
     CDS             complement(221..775)
                     /locus_tag="PK976_00430"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HRC66385.1"
                     /translation="MKKFYHFLLKMLGWTIEGGCPEGNKFIIIQAPHTSLWDFVYGWL
                     FSRAIGIKPKFLIKSKYFFWPLGPILKMLGGVAVYPSKDTRFVKHLVQQIHSSKNFIL
                     VITPEGTRKKVKRWKTGFYQISLETGLPILLGRIDYSNKFIHLGNVFYPTGNMEADLR
                     KIQGYFRASWAKHPERYYEWPPTD"
     gene            complement(815..1267)
                     /locus_tag="PK976_00435"
     CDS             complement(815..1267)
                     /locus_tag="PK976_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66386.1"
                     /translation="MEQNSLKKKIYEVAVETLESAAERTRLAIEEAIQAAQEEVNTLD
                     IYESYRSQYLQKSEMFVSQYNKILEQLDILRKINPEEESQNIEFGSIVITDRQKIFVA
                     VGLGKIKVNGDTWFVISPQVPIFEAMKGKKAGDEFIFNNIKHKILQVS"
     gene            1601..2146
                     /locus_tag="PK976_00440"
     CDS             1601..2146
                     /locus_tag="PK976_00440"
                     /inference="COORDINATES: protein motif:HMM:NF028012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5063 domain-containing protein"
                     /protein_id="HRC66387.1"
                     /translation="MDNKFSHPVYHRNVVEMLALAFEYCRFMEKLESIDKEGLASYLL
                     KVLPLLYVKGELLPEIEAEHPEANERFVIEEEWEGLFNYLRLILDSDDIYYTFDPPAY
                     GWGESLRTSLAEHLADIYQDMKDFVLLYQKNSLAAKENAVAECRRLFLEHWGPRVASC
                     LLAFHFMYHGGEKSYFTDPEQ"
     gene            2210..4171
                     /locus_tag="PK976_00445"
     CDS             2210..4171
                     /locus_tag="PK976_00445"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRC66388.1"
                     /translation="MKLSIIIVNYNVEYFLEQCLHSVRRACKNLEAEIWVVDNNSVDG
                     SLMMLNEKFPEVKIIANKENLGFAKANNQAIREAEGEYILLLNPDTLVEEDTFEKTIA
                     FMGSHPDAGGLGVKMVDGKGKFLKESKRGLPTPSVAFFKMTGLARIFPRSPLFARYYL
                     GHLSDDEIHEVEILSGAFMLLRREVLQKTGLLDEDYFMYGEDVDLSYRILKAGYKNYY
                     FPLTRIIHYKGESTRKSSVNYVFTFYQAMAIFARKHFSAGLARRFNFFINLAIFLRAS
                     LSLLRRFAQYVFLPLLDAALVYGGILVMKNYWEGFIFQGYGQYPPLFVYIILPAYIVV
                     WLLSVYLSGGYDKPFRLGKVIQGIALGTVIILAAYALLPENLRFSRALILFGALWAII
                     SMILTRVILHLSGIDQYHLAVNENKRFLIVGKNNECDRVAEILRNGIHRPAFIGYVAI
                     DPLQQQANGFIGNVQQMNEIIRIYGIDEIVFCASDLTAQFIIDQMLKLQDKSIEFKIA
                     PPESYSIIGSNSISTSNDPFIVDISSITSRRNKRNKRMADLFLVFLLMLTLPVNIWWV
                     ENKFGYLKNLFLVLIGRKTWVGLMDMPGWGEPSSAVKPGVLCPDDAYPSEHLTSQTAF
                     NLNLLYARNYRPLDDFSIVFKGFRNLGRI"
     gene            4337..>4937
                     /locus_tag="PK976_00450"
     CDS             4337..>4937
                     /locus_tag="PK976_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRC66389.1"
                     /translation="MNLKLTKPLAFIDLETTGLNVGHDRIVEISILKVYPDGTTKSYT
                     RRINPTIPIPAEVTRIHGITDDDVKHEPEFSSLGAEIAQFIKDCDLAGYNSNKFDIPL
                     LMEEFLRTGIEFDLKGRKLIDVQNIFHKMEPRNLEAAYRFYCNKDLVNAHSAEADIQA
                     TYEILLAQIERYAGKEVEMTQDSSPLLFDNTTSTLHQLSF"
BASE COUNT         1407 a          971 c         1002 g         1557 t
ORIGIN      
        1 ttttcgcatt ccggtttatt attttggaat cagaaggttg gagagaggca gatatgaagt
       61 ttttgccgag caaatttctt cgaaccctca ccaaagcgaa tttggagaaa tcacaaggaa
      121 ttatgttcgt attcttgaac aagaaatttt aaatcatcct gataattggt tatggtctca
      181 tagacgatgg aagaacttgg ccgaatgata aagattagtc ttaatcggta ggtggccatt
      241 cgtaatatct ttcaggatgc ttggcccatg aggctcgaaa atatccctga atctttcgca
      301 ggtcggcttc catattaccc gtaggataaa agacgttccc aaggtgtatg aatttattgg
      361 aataatcaat ccttccaaga agaatgggca agcccgtttc aaggcttatt tgataaaatc
      421 ctgttttcca gcgtttcacc tttttacgcg taccttcggg agtaattacc aggatgaaat
      481 ttttagaaga atgaatctgt tgcacaaggt gtttgacaaa acgagtatcc ttactaggat
      541 agaccgcaac cccacccaac attttcaata taggtccgag aggccagaaa aaatatttcg
      601 atttgatgag aaacttgggt ttgattccta ttgccctcga aaataaccat ccataaacaa
      661 aatcccacaa actggtatgt ggtgcctgaa tgatgataaa cttatttcct tcgggacaac
      721 ccccttcaat cgtccagcca agcattttga gcaaaaaatg gtaaaatttt ttcatgcggc
      781 ttgttttgga aggatagtca atatagaaaa taaactatga aacttgcaat atcttatgtt
      841 ttatgttgtt gaaaatgaat tcgtctcctg cttttttccc tttcatggct tcgaagatgg
      901 gcacctgagg tgaaattacg aaccaagtat cgccatttac cttaatcttg cccagaccaa
      961 ctgctacaaa tatcttctgg cgatcggtaa tcacaatcga accaaattct atattttgtg
     1021 attcctcttc tggattaatc ttccttaata tatcgagttg ttccaatatt ttattgtatt
     1081 gactgacaaa catttcgctc ttctgaagat attgcgatcg gtaggattca tagatatcca
     1141 aagtatttac ctcttcctga gcagcttgta tggcttcttc aatcgcaagc ctcgttctct
     1201 cggcagccga ttcgagagtt tctacagcta cttcatatat cttcttcttt aatgaattct
     1261 gttccataat cggtttaact tgaataggat aaaaaaaaac ctcccgacaa aggagacatt
     1321 ttatgccggg aaaggtttct ttgcaaatat ataaagaaat gaaatatgca aatgaatttt
     1381 aataaaaaat ttataagtgt ttgcgctcat ctttgccatt cattcctccc tgttttttta
     1441 ccaatttttt tcttctaaaa gctcgattta tgcgccgatt ctttgttcgt tgtttaacga
     1501 tcgtaatttt tggagaaggt tcgggaaaat tttagtcaag gtttttatag agatggtatc
     1561 catagaaagc ttacctttgt tgaggtgaat gtgtaatatt atggataaca aattcagtca
     1621 tcctgtttac catcgtaatg tagtggagat gctggcattg gcctttgagt attgccggtt
     1681 catggaaaaa ttagaaagta tagataagga aggtctggcc tcatatttac tcaaagtatt
     1741 gccgctgcta tacgtgaaag gagaattatt gcccgaaatc gaagctgagc atcctgaagc
     1801 caatgaacgt ttcgtgatag aagaagaatg ggaagggctt tttaattatc tcagactcat
     1861 attagattcc gacgatattt attacacctt cgatcctccg gcttatggct ggggagaaag
     1921 tttaaggacc agtctggcag agcatctggc cgatatttat caggatatga aagatttcgt
     1981 gttattgtat cagaagaatt cgcttgctgc caaagaaaat gcagtagccg aatgccgccg
     2041 gcttttcctc gaacattggg gccctcgtgt cgcatcttgt ttgcttgctt tccattttat
     2101 gtatcatgga ggcgaaaaat cttattttac cgacccagag caataagata ttttatgcta
     2161 tcgttaatta ggctaacata ttcatttatg cttttctacc tttccttcga tgaaactttc
     2221 aatcattatc gtcaattata acgttgaata ttttcttgaa caatgcttgc attcagtaag
     2281 gcgagcctgt aaaaacctcg aggcagaaat atgggtggtc gataacaata gcgtggatgg
     2341 ctcgttgatg atgctgaacg aaaaatttcc cgaagtaaaa ataattgcca ataaagaaaa
     2401 cctggggttt gccaaagcaa ataatcaagc tatacgtgag gcagaaggag aatatattct
     2461 cttgctgaat cccgataccc tagtggaaga ggacacattc gaaaaaacca tagcctttat
     2521 gggttcacat cctgatgcag gtgggttagg agtgaagatg gtagatggca agggaaaatt
     2581 cctgaaagaa tctaaacgag gcttacctac tccttcggtg gcttttttca agatgacagg
     2641 actagcaaga atatttcccc gcagtccttt gtttgcccgt tattatcttg gccatctttc
     2701 cgatgatgag attcacgaag ttgaaatcct ttcgggtgct tttatgcttt tgagacgtga
     2761 agtgcttcaa aaaaccggtt tgctcgatga agattatttc atgtatgggg aagacgttga
     2821 tttgtcgtac agaattttga aggccggata caagaattat tactttccac tcacgcgtat
     2881 cattcattac aagggtgaaa gtacacgtaa gagctcggtc aattatgtct ttacgtttta
     2941 tcaagccatg gccatttttg ccagaaagca tttttcggcc ggcttggcac gcaggttcaa
     3001 cttttttatc aatctggcta tttttttgag ggctagtctt tctcttttgc ggcgatttgc
     3061 tcaatacgtt tttttaccct tgctggatgc tgccttggtt tatggtggta ttctggtgat
     3121 gaagaattac tgggagggtt ttatctttca gggatatgga caataccccc cacttttcgt
     3181 ttacatcatt ttaccggctt atattgttgt ctggctactt tcggtgtatt tgagcggagg
     3241 ctacgacaaa ccctttcgtt taggaaaggt tattcaggga attgcgctgg gtactgtgat
     3301 tattttggct gcttatgcgt tattgcccga aaatttgaga ttttcgcgcg ctctgattct
     3361 ttttggtgca ttatgggcta ttatcagcat gatactgact cgcgtaattc ttcatttgag
     3421 tggtatcgat caatatcacc tggctgtaaa cgagaataaa cgatttctga tagttgggaa
     3481 aaataacgaa tgcgatcggg tggccgaaat cctgcgtaat ggaatccaca gaccggcttt
     3541 tattggttat gtagccatcg atccccttca gcaacaagcc aacggtttta tcggaaatgt
     3601 acaacaaatg aacgaaatta ttcgaatcta tggtatcgat gaaatcgttt tttgcgccag
     3661 cgatcttacg gctcaattca tcatcgatca aatgttgaaa cttcaagata aatccataga
     3721 attcaaaatt gcacctcccg aaagctattc tatcattggc agcaattcta taagcacttc
     3781 gaacgatcct tttatcgtcg acatcagcag cataaccagt cgtcgcaaca agcgtaataa
     3841 gcggatggca gatctcttcc tcgttttctt gctaatgctc actcttcctg ttaatatttg
     3901 gtgggtcgaa aataaattcg gatatctgaa aaatcttttc cttgtgctaa taggtcgcaa
     3961 aacctgggta ggcctcatgg atatgccggg ctggggagag ccatcttctg cagttaaacc
     4021 tggagtttta tgccctgacg atgcctatcc ttccgaacac ttaacatctc aaaccgcgtt
     4081 caaccttaat ttgctctatg cccgaaatta cagacctttg gatgatttca gtatcgtatt
     4141 caaaggcttt cgcaatcttg gacgaattta aatcggctga tttcgtatga atctcgtctt
     4201 acttattgat gacagaaaac tcaaaaaccc aaatttttcg tttatggaat cgtcgatggt
     4261 ttatcatgga ccaattcagg tctatacgtt atttattcta attttgcctt agaaatgaat
     4321 tatttgctaa aggaaaatga atttaaaact aaccaagcca cttgcattta tcgatcttga
     4381 aaccactggt ttaaacgttg gccacgatag aattgtcgaa atcagtattc tgaaggtata
     4441 tcccgatggg acaaccaaat cctatactcg ccgcataaat cccaccattc ctatcccggc
     4501 cgaggtgaca cgcatacatg ggattaccga cgatgacgta aaacatgaac ctgaattttc
     4561 gagtttaggt gcagagattg cccaatttat aaaggattgt gatctggcag gttacaatag
     4621 caataaattc gacatacctc tactcatgga agaatttctt agaactggga tagagttcga
     4681 tttaaaaggc aggaagttga tcgatgtgca gaacattttt cataagatgg aacctcgaaa
     4741 cctggaggct gcatatagat tctattgcaa taaggacctt gtaaatgccc actcggccga
     4801 agccgacatt caggctacct atgaaatcct tctggctcag attgaacgat atgccggcaa
     4861 agaagtcgag atgacccaag attccagccc gctcttattc gacaacacca cttcaacctt
     4921 gcatcagctt tcttttc
//
LOCUS       DAORPD010000018         4839 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_3111_length_4839_cov_2.363712, whole genome shotgun sequence.
ACCESSION   DAORPD010000018 DAORPD010000000
VERSION     DAORPD010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4839
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3111_length_4839_cov_2.363712"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1759
                     /locus_tag="PK976_00455"
     CDS             <1..1759
                     /locus_tag="PK976_00455"
                     /inference="COORDINATES: protein motif:HMM:NF015926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HRC66390.1"
                     /translation="SEFLKEIDFQGVDYDLLSREKLVEVLKSLVDNDDINGIKTQVGL
                     IKVTFIRKTKERQKQELESYIASGGREDEFQYQPDDIEHQFDAVFDIYKEKKLQFAEE
                     LERQKQQNLEAKLAILDELRALINSEETLKKTYDEFRNLQEKWRQIGMVPKSEVNTLW
                     QNYHFLVEKFFDKVKINKELKDLDLKKNLESKIELCEKAEELLLEPNVMKSFKELQKY
                     HEQWREIGPVPSEKRDEIWERFKAATEKINARRREYYDKLQDELRNNYESKQALIEKA
                     REIIETPAETIRQWQENTRAIDELFKIWRSVGPAPRKVNDQIWNQFKELLDQFYKQKS
                     EFFSKIKEQQLHNYNLKLDLCVQAEALRDSTDWKNTTKEMINLQKEWKKVGPVPRKQS
                     EKIWKRFRTACDQFFKAKEEYFSNIHQNEKENLRLKEELIKQVEEYPYTDDKAENLRV
                     IKEFQRKWVEIGYVPLNEKERLQNSFRKAIDKQLDRLNISPIEVDAMSYKLRFEQIKD
                     LPDGHKTIIREINFLQNKAAKMTEDVTLWENNLGFLASSKNADILRQEFERKIHKTKQ
                     EIKLIEAKVKFLKEELNKK"
     gene            2096..3121
                     /locus_tag="PK976_00460"
     CDS             2096..3121
                     /locus_tag="PK976_00460"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HRC66391.1"
                     /translation="MNKILDSFDRKSPLKWLVVIGLVVIIGFIIHAHIEKPLIDEIIG
                     DDFVKQGDMKVVPVPIPKKLSFAGEEVPLNLFYVRESLDRELTVNTYWHSQSLLLFKR
                     AHRYFPVIEPILKKNDIPEDFKYMILAESGFLHNISIAGAAGYWQFIKSTAQEYGLEV
                     NQYVDERYNLEKSTEAMCKFLKKAYEKYGTWTLAAAAFNGGMGRFDRIVQSQKTTNYY
                     EMYMNQETSRYIFRILAFKLIFEHPERYGYYFKKENLYPPIPSKEIVVKEDISNLIEF
                     AQKQGINYRILRELNPWISDTSLIVSPNKRYRIKIPTGNYKNYDEMIREEVNKEASVP
                     DNSYDKD"
     gene            3108..>4839
                     /locus_tag="PK976_00465"
     CDS             3108..>4839
                     /locus_tag="PK976_00465"
                     /inference="COORDINATES: protein motif:HMM:NF036483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRC66392.1"
                     /translation="MTRIEEGIPKDILLQKEDNLEVYGARVHNLRNIDVIIPRDKFVV
                     ITGLSGSGKSSLAFDTIYAEGQRRYLETFSAYARQFIGNLERPDVDKIVGLSPVISIE
                     QKSSGSNPRSTVGTITEVYDFMRLLFARIAKAYSYMTGELMVKYSESNLIEIIRKEFE
                     DCKVDILAPLVRGRKGHYRELIDSLRKQGYSRIRVDGDIKSLSEIRQLDRYKTHDIEL
                     IIDRIKIGGSSEERLTSSVQTALKQGKGTLIVFNESDGSMRYLSRNLMCPTTGIAYED
                     PEPNSFSFNSIYGACPKCSGLGRISEIDLEKIFPDKNKTTRQGGIAPLAEYRDNWIFR
                     QIEGIGQIYGFHLDTPIKDLSEQALNAILYGVDGGFKVRKEYDSGVIQTYTLEFDGIV
                     DFIIRQYQESETASIRKWVGEFLNKVTCPVCNGSRLKKESLWFKVDGKNIAELAMMDL
                     GSLDHWFGQLPERLQGNDRIIGQEIIREIRSRLWFLLELGLGYLTCDRPAYSLSGGES
                     QRIHLATQIGSQLTGVLYILDEPSIGLHQRDNHRLIKALKNLRDIGNSIIVVEHDKEM
                     IVSADYVVDLG"
BASE COUNT         1585 a          867 c         1084 g         1303 t
ORIGIN      
        1 atctgaattt ttgaaagaaa tagattttca gggagttgat tatgatctgc tttcgagaga
       61 aaaacttgtg gaagtgctca aatcgcttgt agataatgat gatattaatg gtatcaagac
      121 ccaggtaggg cttattaagg ttactttcat acgtaaaaca aaggaacgtc agaagcagga
      181 attagaatca tatatagctt caggagggag agaagatgaa tttcaatatc agcccgatga
      241 tattgaacat caatttgatg ccgttttcga tatttacaag gaaaagaagc ttcagtttgc
      301 ggaggagctg gaaaggcaga agcagcagaa tttggaagcc aaactggcca ttctggatga
      361 attgagggct ttgattaatt ctgaagaaac actgaagaag acttatgatg aattcagaaa
      421 tcttcaggaa aaatggcgtc aaataggaat ggttcccaag tcggaagtta ataccctttg
      481 gcagaattat cattttttag ttgagaaatt tttcgacaag gtaaaaatca acaaagaatt
      541 aaaagacctt gatcttaaga agaatttgga aagcaagata gaactttgcg aaaaggcgga
      601 agagctttta ctggagccta atgtaatgaa gtcgttcaag gagcttcaga aatatcacga
      661 gcagtggcgt gagattggcc ctgttccaag tgaaaaaaga gatgagatat gggaaagatt
      721 caaggcagct accgaaaaaa tcaatgcccg acgaagagaa tattacgata aactacagga
      781 tgaacttcgc aataactatg aatcgaaaca ggccttgatc gaaaaagcca gggagattat
      841 cgaaacacct gcggagacta tccgtcaatg gcaggaaaat acacgtgcca tcgatgaatt
      901 attcaagata tggcgtagcg ttggccctgc acctcgaaaa gtgaacgatc aaatctggaa
      961 ccagttcaaa gagcttctcg accagtttta taaacaaaaa agtgagttct ttagcaagat
     1021 aaaagaacaa caattgcaca attacaacct gaagctcgat ttatgtgttc aagcagaggc
     1081 tttaagggat agcaccgatt ggaaaaatac gaccaaggaa atgatcaatc tgcagaaaga
     1141 gtggaaaaag gttggaccgg ttcctcgcaa gcaatctgaa aaaatctgga aacgcttccg
     1201 taccgcctgc gaccaattct ttaaggctaa ggaagaatat ttttccaaca ttcatcaaaa
     1261 tgaaaaagaa aaccttcgtc tgaaagaaga actcatcaaa caggtggaag aatatcctta
     1321 taccgacgat aaagccgaaa atttacgggt aatcaaagaa tttcaacgta aatgggtcga
     1381 gataggatat gtacccctga atgaaaaaga acgcctgcaa aatagcttca gaaaagcaat
     1441 cgataaacaa ctcgaccgcc tcaatatcag ccccatcgag gtagatgcca tgtcgtacaa
     1501 actgcgattc gaacagatta aagacttgcc cgatggacat aaaaccatca tcagagaaat
     1561 taacttcctg caaaacaaag cagcaaaaat gaccgaagat gttaccctct gggaaaacaa
     1621 tctgggcttt ttggcgagtt cgaaaaatgc cgatatcctc cgtcaggaat tcgaacgcaa
     1681 aattcataaa accaaacagg aaatcaaact tatcgaggca aaggtgaaat ttctgaaaga
     1741 ggaactgaat aagaaataaa tcagtttagc aatttgtttt cttgttgtgc tttcaactga
     1801 agtgcaaaag agaaaagtcc taaaaattat ggaaaaagat gctcagctct ttattcggtg
     1861 aatgatctgt tcatcagcgc aggttcatat ttgagctttg aattcaaagc catttcggca
     1921 tgcggtaaac tctggcctga aatttgagtt ttgtaacctg ctttcatcac tggttttagt
     1981 attcataatg aactcctgaa atgggagata acttggttac ttatcataaa aggagatatt
     2041 gtataacttt gcaagctttc gtaaaaacaa ttttggttta ctttgattct tgataatgaa
     2101 taagatactt gattcttttg ataggaagtc gcctttaaaa tggcttgtgg ttataggttt
     2161 ggtcgtgata ataggattta tcatacatgc ccatatagaa aagcctctaa tcgatgagat
     2221 cattggggat gattttgtaa aacaaggtga catgaaagtc gtacctgtcc caattccgaa
     2281 aaaactttca tttgctgggg aagaagttcc tctgaatttg ttctatgtta gagaaagcct
     2341 cgacagagag ctcacggtga atacatactg gcattcgcag tcattattgt tgtttaaaag
     2401 agcccatcgt tatttccctg taatcgaacc tattctaaag aagaacgata ttcctgaaga
     2461 cttcaaatac atgatactgg cggagagtgg ttttcttcat aatatttcga tagctggtgc
     2521 tgctggctat tggcaattca taaaatccac tgctcaggaa tatggccttg aagtcaacca
     2581 gtacgtagat gaaagatata atctggagaa atctaccgaa gccatgtgca agtttttaaa
     2641 aaaggcctac gaaaaatatg gaacatggac tttagctgcg gctgccttca atggaggcat
     2701 ggggcgtttc gaccgtattg tacagtcgca gaaaactacg aattattacg aaatgtatat
     2761 gaatcaggaa acttcgaggt atatttttcg gattctggct tttaagctta tttttgaaca
     2821 tcccgagcgg tatggttatt attttaagaa ggaaaacctt tatccgccaa ttccaagcaa
     2881 agaaattgta gtaaaggaag atatctccaa tcttattgaa tttgctcaaa aacaaggcat
     2941 taattatcgc attttgcgtg aattgaatcc atggatatca gacaccagtt tgattgtttc
     3001 accgaataag agatatagaa ttaagattcc tacagggaat tataaaaatt acgatgaaat
     3061 gatcagggaa gaggtcaata aagaagcatc tgtccccgat aatagttatg acaaggattg
     3121 aagaaggtat accgaaagac atattactgc agaaagagga caatctggag gtttatggtg
     3181 cccgtgtgca taatttgcgt aatatcgacg ttattattcc ccgagataaa tttgtggtga
     3241 ttaccggttt gagcggcagt ggaaaatctt ctcttgcttt tgataccatc tatgcagaag
     3301 ggcaaagaag atacctcgag acattttcgg cttacgcccg tcaattcatt ggcaatctcg
     3361 aacgccccga cgtggataag attgtgggct taagtccggt gatttcgatc gaacagaaga
     3421 gttcgggttc taatccacgc tctaccgtcg gaaccattac cgaggtttat gatttcatgc
     3481 gtctgttatt tgctcgcatt gcaaaggctt attcctatat gacgggcgaa ctcatggtca
     3541 aatacagcga aagcaacctt atagaaatta ttcggaaaga gtttgaagat tgcaaggtag
     3601 atattcttgc tcccttagtt agagggcgta agggtcatta ccgcgaactc atcgattcgt
     3661 tacgcaaaca gggatattcg agaattaggg tagatggcga tataaaatct ttatcggaaa
     3721 ttcgtcagtt agaccgttac aaaacgcatg atatcgagct catcatcgat aggataaaaa
     3781 ttggtggaag ctcggaagaa cgtctgacct cctcagttca aactgcattg aaacaaggca
     3841 agggaaccct tatcgttttc aatgaatctg atggcagtat gcgctatctc agccgcaatc
     3901 tgatgtgtcc gaccactgga attgcttacg aagaccccga accgaattct ttctcgttta
     3961 attctattta tggagcatgc ccaaagtgta gcgggcttgg ccgtatcagc gagatcgatc
     4021 tggaaaagat tttccccgac aagaataaaa ccacacgtca gggtggaata gccccactgg
     4081 ccgaatatag agataattgg attttcaggc agatagaagg aattggccag atctatggtt
     4141 ttcatctcga tactcctatc aaagaccttt ctgaacaggc tttaaatgct atcctctatg
     4201 gagtcgacgg aggcttcaag gtcagaaagg aatacgatag tggagttata cagacttata
     4261 cccttgagtt cgatggtata gtcgatttta ttattcgaca gtatcaggaa tcggaaactg
     4321 cctcgattcg taagtgggta ggagaattcc tgaataaggt gacctgcccg gtgtgcaatg
     4381 gaagccgatt gaagaaggaa tcattatggt tcaaggtaga tggtaaaaat attgccgaat
     4441 tggccatgat ggacctcgga agtctggatc attggttcgg acaattaccc gaaaggcttc
     4501 aggggaatga tcgcatcata ggtcaggaaa ttataagaga gataaggtcg cggttgtggt
     4561 ttcttctgga acttggctta ggctatctta cctgcgacag acctgcctat agtctatcgg
     4621 gtggtgaaag ccagcgcatt catctggcta ctcaaattgg aagccagctt acgggtgttt
     4681 tatacatact tgacgaacct agcataggtt tgcaccagag agataatcat cgcctcatca
     4741 aagccttgaa gaatttgcgc gatattggca attccattat tgtagtcgaa catgataagg
     4801 agatgatagt atcggccgat tatgtggtag acctcggtc
//
LOCUS       DAORPD010000019         4677 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_3248_length_4677_cov_2.831891, whole genome shotgun sequence.
ACCESSION   DAORPD010000019 DAORPD010000000
VERSION     DAORPD010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4677
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3248_length_4677_cov_2.831891"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..1027)
                     /locus_tag="PK976_00470"
     CDS             complement(68..1027)
                     /locus_tag="PK976_00470"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HRC66393.1"
                     /translation="MENYFRNLKLIEILIKWKWHLSIIVVATIVLSVIFSSPSFIKPR
                     YKSSAVLYPSNIKEYSDESLTEQMLQIMQSTDIRDSLIQKFSLIKHYKIDTTKKYWKS
                     NLYWNYNHNVKINKTQYEAVLIEVYDFDPYQARDMVNSIINFYNRKIASLHNEKFNEV
                     VAMYERYLTRKSHELDSLKDLMRDLGVNYNLLDFSFQTQQVVKGYLGTTDFGGGRINT
                     NEVQRIRKNMEEKGGDLIMLQELIRQEAANYALIKKDYDRAYMDHDRQFTYSNIISSP
                     QVPDKKATPVRWIIVLVSTLSVLLLSVIVIGIIENSRLKKENA"
     gene            complement(1120..2157)
                     /locus_tag="PK976_00475"
     CDS             complement(1120..2157)
                     /locus_tag="PK976_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66394.1"
                     /translation="MTKDYSSEKLFLFLSIWGKPLLIVTIIAFFASLIFSSSYFIKPK
                     YKSTLIMYPVSTSSISKALLSNTYGIQQDVLEFGAEEQAEQMLQILNSNLIRDRIIAK
                     YDLANHYGIKPREKYRLTKLYKEYENNITFRRTENMAIKVSVLDHDPQTAADIANDIA
                     ALFDSTKTAMQRERALKAFHIVEGEYLKKQAEIQQMEDSLVKLRELGVFDYETQTEMI
                     NQQLAVEIARGNTRGIQALQAQLDVLARYGGAYVSIRNQLEFEKKQLSDLKAKYEEAR
                     VDAFEFLPQKFVVTTAYKAEKKSYPIRWLIVTLTVISSLFLATIIIIVLENYSSLKAK
                     KKISNPIILSP"
     gene            complement(2258..3676)
                     /locus_tag="PK976_00480"
     CDS             complement(2258..3676)
                     /locus_tag="PK976_00480"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HRC66395.1"
                     /translation="MGIDRSVQNLVGFEEYGFYTTILNFSFLFNILLDFGITNYNNRN
                     IAQNRHLLTKHFSNILVLRFLLAALYFVVIMLTGLIIGYNSRQMSLLAVVGFNQFLLA
                     LILYLRSNISGLLMFRTDSILSVLDRFLMIVFVGYLLLNQHYRLHFRIEWFVYTQTLA
                     YLLTCLVALMIVWRKAGKTKLHWNFPFFVMIIKQSIPFAILALLMATYNRIDPVLLER
                     LIPDPQGNHQVGIYAAAYRLLDAFNMVAYLFSVFLLPTFATLIKHKGDIIPFIKFSFS
                     ALFVFSILLAFTALYYSPELMQLLYPQHTDESLAEFQARLNQSSLIFTLLMFAFISIS
                     SSYVFGTLLTANGNLKQLNIIAAICVAINLSINLLLIPRIMATGSAIASLSAQTFSAI
                     AQVILVVKIFKIRVNKRYLTTLVVFTLVSLIAGFVAKQIGLSWLHGMCLLWVMGLITA
                     MGLNLINVKMLMKAMTEKANNI"
     gene            3835..>4677
                     /locus_tag="PK976_00485"
     CDS             3835..>4677
                     /locus_tag="PK976_00485"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HRC66396.1"
                     /translation="MRILVNTRLLLYDKLDGIGWFAFETLKRITAEHPEYEFIFVFDR
                     PFHPDFVFSSNVRAVVAPPPTRHPVLIFLWFELSIPFLIRKFRPDVFLSPDGFLSLSA
                     KVKSLTVIHDLNFEHNPQDLPWKYRVFYRFFFKRYAKKASRIATVSEFSASDISQHYS
                     VIRDKIDVVYNGVAEGFKPLPPERIREIREEFSEGDPYFIFVGSIHPRKNIINLLKAF
                     DLFRLRTSYPMNLLIVGKKQWCSSSIEETHRKMKYQKDVIFTGRLDVDKLQQVVASAF
                     ALTYV"
BASE COUNT         1397 a          830 c          980 g         1470 t
ORIGIN      
        1 atgaggtata accacttcac cttgtatttt tcgatagagc tgattttatg aaaagcagct
       61 gacataatta ggcattttct ttttttagcc ggctgttctc aataatgcca atcactataa
      121 ctgataagag cagaacactc aaagtagata cgagaacgat gatccatctt actggagttg
      181 cctttttatc gggtacctga gggcttgata tgatgttcga ataggtaaat tgacggtcgt
      241 gatccatgta agctcgatcg taatcttttt taattaaagc ataattggcg gcttcctgtc
      301 ttatcaattc ctggagcata ataagatctc cacctttttc ttccatgttt ttgcgaatac
      361 gctgcacttc gttcgtattg attcttcctc ctccgaagtc ggttgttccc agataaccct
      421 tcaccacttg ctgcgtttga aagctaaagt cgagcaggtt gtaattcacg cccaagtcgc
      481 gcatgaggtc tttgagggaa tccaactcat gtgattttcg tgtaagatag cgttcgtaca
      541 tggccaccac ttcgttgaat ttttcgttat gaaggctggc aatcttacga ttatagaaat
      601 tgataataga attcaccatg tctctggcct gatagggatc aaaatcgtac acctcgataa
      661 gaacggcttc gtattgtgtt ttgttgattt ttacgttgtg gttgtaattc caataaagat
      721 tcgatttcca atatttcttg gtggtatcga ttttataatg tttgataaga ctgaattttt
      781 gaattaagct gtcgcgtata tcggtggatt gcatgatctg caacatctgc tcggtgaggc
      841 tttcatcgga atattctttg atattggaag gatacaatac ggctgaagat ttgtatcggg
      901 gtttgatgaa tgaaggactc gaaaagatta ctgagagcac gatggtagcc acgactatta
      961 tacttaaatg ccatttccat ttaatcagta tttcaatcag tttgaggttc ctaaaataat
     1021 tttccatggt cagcaaatta aaggtaattg gttcaggtat gaagttttta agaaaaatga
     1081 gaaatttatg agatttattg tgcagataag tatcaaaatt tagggggaaa gtattattgg
     1141 gtttgaaatt ttttttttag ctttcaaaga ggagtagttt tcaaggacga tgatgatgat
     1201 tgtcgccagg aataaggatg aaataacagt aagtgtaacg atgagccatc ggatgggata
     1261 ggattttttt tcggccttgt aagctgtagt aaccacgaat ttttgtggca ggaattcgaa
     1321 ggcatctaca cgggcttctt cgtatttggc cttgaggtca ctcaattgtt ttttttcgaa
     1381 ttcgagctga tttcgtatcg atacataagc tcccccataa cgtgcaagga catcaagctg
     1441 ggcttgtagg gcttgaatcc cgcgagtatt tcctctggca atctccacag ctaattgctg
     1501 attgatcatt tcggtttggg tttcatagtc gaacactcct aattctctga gtttaaccag
     1561 cgaatcttcc atttgctgaa tttcggcttg ctttttaaga tattctcctt ctacaatatg
     1621 aaaagccttc agggctcttt cgcgttgcat ggcggttttt gtactgtcga acaaggctgc
     1681 aatatcattg gctatatcag ctgctgtctg agggtcatga tcgagaacgg aaacctttat
     1741 ggccatattt tcagtgcggc gaaaagtaat gttgttttca tattctttat aaagcttggt
     1801 gagcctgtat ttttcgcggg gctttattcc ataatggttg gctaaatcat attttgcaat
     1861 gattctgtct ctaatgagat tcgagttgag tatctgcaac atttgttcgg cttgttcttc
     1921 ggcaccgaat tcgagtacat cctgttgaat tccataagtg ttggataaca atgccttcga
     1981 aatactgctc gtcgaaacag gatacatgat cagggtcgac ttatactttg gcttgataaa
     2041 atatgaggaa gaaaatatga gtgaagcaaa gaatgcaata attgttacaa ttagcaatgg
     2101 tttcccccat atcgaaagga atagaaataa cttttccgaa ctgtagtctt ttgtcacttt
     2161 tgttgtatca tctgtcatat atggccttaa ctaggcaatg ttaaatgagg ggtcaaaatt
     2221 aagaaaaaaa gaatataaat cgataaagaa ttgatgctta tatattattt gccttctcgg
     2281 tcatggcctt catcaacatt ttaacattga tcaaatttaa gcccatggcg gttataaggc
     2341 ccatcaccca taaaaggcac atcccatgta accatgaaag ccctatttgt ttagcgacaa
     2401 aacccgctat aagtgaaacc agtgtaaaaa caaccaatgt ggtgagataa cgtttattta
     2461 cacgtatctt gaatattttg actaccagaa taacttgagc aatcgcagaa aaggtttgtg
     2521 ccgagagact tgcaattgcc gaacccgtag ccatgatacg aggaataagt aatagattga
     2581 tagaaagatt tatagctacg catatggcag ctattatatt cagttgtttt agatttccgt
     2641 ttgctgtgag taaggttcca aaaacgtaag aagacgatat ggaaatgaat gcaaacataa
     2701 gaagagtgaa gatcagggaa gattgattca gacgtgcttg aaattccgcc aaactctcgt
     2761 ccgtatgttg tggatacaac aattgcatta attcgggaga gtaatagagt gcagtaaagg
     2821 ctaacaagat tgaaaagacg aataatgcag aaaatgaaaa ttttataaac ggtataatgt
     2881 ctcctttatg tttgattaaa gtggcaaagg tgggtaaaag aaaaactgag aagagataag
     2941 ctaccatatt gaaagcgtcg agcaaacgat aggcagcggc ataaattcct acttgatggt
     3001 tgccctgggg atcgggaatg agcctttcga gcaacacggg atcgattcga ttgtaagtag
     3061 ccattaaaag agccaggatt gcaaagggaa tgctctgctt gataatcatc acaaagaatg
     3121 gaaaattcca gtgtagcttg gtttttcctg cttttctcca aacaatcatc aaagctacga
     3181 ggcatgtgag caaataagct aacgtttgcg tatatacgaa ccattctatt cggaaatgta
     3241 acctgtaatg ctggttgagt aataaataac ccacaaaaac gatcatcaga aatctgtcga
     3301 gtaccgaaag gatgctgtcg gtgcggaaca tgagcaaacc tgaaatattg ctacgtaagt
     3361 aaaggatcaa tgcaagtaga aattggttga atccaacgac tgccaataaa ctcatctgtc
     3421 ggctgttgta cccaatgatc aaccccgtga gcatgattac tacaaagtag agagccgcta
     3481 aaagaaatct caatacaaga atattggaaa agtgtttggt gagtaaatgt cggttttggg
     3541 caatatttct attattataa ttggttatgc caaaatcgag cagtatgtta aaaagaaagg
     3601 aaaaatttaa aatagtggta taaaacccat actcttcaaa acctacaagg ttctgaacac
     3661 ttctgtcgat tcccaatatc cagaaaggtt tgattaggag attgagaaaa agtaaaagcc
     3721 acaggttgga aataaatttt ttttgcatcc ctgcaaaagt aggaaatacc ctttcttctt
     3781 gtcagaattg tctaaaattg ttaagattcc tatttttgca aaaatgaaaa tcctttgcgt
     3841 attctggtca atacaagatt gcttctgtat gataaactcg atggtattgg atggtttgct
     3901 ttcgaaactc taaaacgtat tactgccgag catcctgagt atgagtttat ttttgtgttc
     3961 gacaggcctt ttcatcccga ttttgtcttc tcatccaatg taagagccgt ggttgctccc
     4021 cctcccacac gccatcctgt tttgatattt ttgtggttcg agctaagcat tcctttcctg
     4081 attcgtaaat tccgtcccga tgtatttctg tcgcccgacg gttttttatc gctttcagct
     4141 aaagtgaaat ctcttacagt gattcatgat ttgaattttg aacataatcc tcaggattta
     4201 ccttggaaat atagagtttt ctatcgtttc ttttttaaac gctatgcaaa aaaagccagt
     4261 cgtattgcta cagtatccga attttcggcc agcgacatca gccagcatta ttccgtcatt
     4321 agagataaga tcgacgtggt atacaatggt gtagccgaag gattcaagcc tttgccgcca
     4381 gaaagaatca gagaaattag agaagagttt tccgaaggag acccctattt tattttcgtg
     4441 ggttctatcc atccgcgaaa aaatatcatt aatcttctga aggccttcga tttattcagg
     4501 ctgcggacat cttacccaat gaatctgttg atcgtaggga agaagcaatg gtgtagttct
     4561 tccatcgaag aaacccatcg aaagatgaaa taccaaaaag atgttatttt tacgggaagg
     4621 ttggatgtgg ataaacttca gcaagtggtt gcttctgcct ttgccttgac ttatgtg
//
LOCUS       DAORPD010000020         4646 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_3278_length_4646_cov_2.604443, whole genome shotgun sequence.
ACCESSION   DAORPD010000020 DAORPD010000000
VERSION     DAORPD010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4646
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3278_length_4646_cov_2.604443"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(155..1147)
                     /locus_tag="PK976_00490"
     CDS             complement(155..1147)
                     /locus_tag="PK976_00490"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HRC66397.1"
                     /translation="MPDFSGDAEMNLMKSTTWVNIALLLVFSGGKTLAQQEPQFTHNV
                     FTRMAINPAYAGSSGDISVTGLMRQQWVGFKDMDGEKVAPQTYLLTADMPVSLVHGGL
                     GISITSDQLGFENNVGVRLNYAYRTVAGNGDLAFGPVIGFLNKSIDFGKLKPTQSGDP
                     VLGSAEESTMMMDVGLGAYYEVKNKYFLGISTSQLIQTSSSIGVDATNYNLKRHYYAH
                     GGYIYTLPDNPLIKLQPSLFIKTDFVSTQFDVNLSALYNERFCGGISYRLQDAIAFLL
                     GLSYKSLIFGYSYDLTTSRLGSAGSSGSHEIFLNYRFKLQLDKNPRSYRNTRYL"
     gene            1524..2429
                     /locus_tag="PK976_00495"
     CDS             1524..2429
                     /locus_tag="PK976_00495"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HRC66398.1"
                     /translation="MQKRRAIAKEIIVSYGLIVIGAFIMAAGFVLFINPYKIVPGGVY
                     GIGIVVHYITKGLIPFWPEGIPIGLVGLSLDIPLILLGMHLLGTKFGTKTILGSFLMS
                     SFMMVLTYFIGEHDPLGLQEDILLSCIFGGLTVGLGLGLIFRSRATSGGSDIIAMILS
                     KYSKLPVGQLLILVDSTIVLIGLATFKDWRIPLYSWIVIYITGKVIDLVVEGVNFEKS
                     VFIISDHPEAIRQKIIYDLRRGGTFIEGEGMYNGKPRRIIHTVMSRREVSLLLDHVRR
                     IDPDAFITVTDASEILGEGFKSLNE"
     gene            2454..3347
                     /locus_tag="PK976_00500"
     CDS             2454..3347
                     /locus_tag="PK976_00500"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HRC66399.1"
                     /translation="MAFISVETFPSKRWFAAYGKILLGSLLVAFGYVLFITPYKIVPG
                     GVYGISIIIHYLLGTPVGLTALAFNIPITLLATKVLGPRYGAKTVTGFILTSLFIDGL
                     TYFLGNDPLKISDEMLMSSLYGGLLIGTGVGLIFRARASCGGTDVIAMMISKFIHQPV
                     GQIMIYVDSLIVLGGFIAFKDWKIPLYSLVTIFMVGRMVDVVMEGLNYNKCLLIVSDK
                     QKEISQRIIHDLKRGGTIFQSKGIYTGKEHSLIFTVMSRSEMIKLKSFIRQIDPKAFI
                     TVMDTSEILGKGFRPLNENID"
     gene            3653..4327
                     /locus_tag="PK976_00505"
     CDS             3653..4327
                     /locus_tag="PK976_00505"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRC66400.1"
                     /translation="MKTIRKILCFSVLLLIGSSIYAQDNEPGGFRKFSIGFDFFTDFW
                     QNPPSVLNAKWYNRGSNVNILINNKMGESRFDFSYGVSFGSHNLYTDAWIKDVNQKSV
                     FEKIPDTLDHKKYKITLAYCDFPLEFKYRAKNKWRYYAGIKLGFLINHHSKYIGLDPE
                     TYAYNVKIKRDDVRYVNFWRYVVYARVGYKWFNVFGQYSLNHTFKDGQGPDMYPISVG
                     ISLMPF"
     gene            complement(4314..>4646)
                     /locus_tag="PK976_00510"
     CDS             complement(4314..>4646)
                     /locus_tag="PK976_00510"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRC66401.1"
                     /translation="NPLLLTLPLILIVSILIDWAVNKFWKISIHMTSLGGLSSIIIFF
                     SWHRHDIFPRIVLILLLIGILLGWARWRLKAHTFMQVILGWINGFVIALACLKYLPDV
                     FSSLFKMA"
BASE COUNT         1400 a          842 c          922 g         1482 t
ORIGIN      
        1 ggtcaattcc ccgctgcctg tattgcggca gctagccaaa gcaatgattg caacaactaa
       61 atacagcagt tttttcattt ttatcaacgg ttataatcga ttaaaactta ttttttcaaa
      121 tcaaaaattc tgatggcaca aaaataaaaa aaactcataa gtatcttgta tttctataac
      181 tgcgaggatt tttatcgagc tgcagtttga aacggtaatt cagaaaaatt tcatgactgc
      241 cagaacttcc cgcacttcca agacgagaag tagttaaatc gtaggagtag ccaaaaataa
      301 gacttttgta actcaaccct aataaaaatg caatagcatc ctgcaatcgg tagctgattc
      361 caccacagaa tctctcattg taaagggcag atagatttac gtcaaactgt gtagaaacga
      421 agtcggtttt tatgaagaga ctgggttgaa gtttgattaa aggattatcg ggtaaagtat
      481 aaatatatcc cccatgagca tagtaatggc gtttcaggtt gtagttcgtt gcatcgacgc
      541 cgatgctcga gcttgtttga ataagctgtg aagtagaaat gcctaagaag tatttgtttt
      601 tcacttcgta ataagccccc aggccaacat ccatcatcat tgtactttcc tcagcactgc
      661 ccaacacagg atcaccactc tgggtaggtt tgagtttgcc gaaatctata gatttattta
      721 aaaaaccaat caccggccca aatgccaggt ctccatttcc ggcaacagtt ctatatgcat
      781 aattcaaacg cacacctaca ttattttcaa agccaagctg atcggaagtg atagagattc
      841 caagacctcc gtgaaccaaa cttacgggca tatctgccgt gagaagataa gtttggggtg
      901 ccaccttttc accatccata tctttgaacc caacccattg ctgacgcatc aaacctgtta
      961 cagaaatatc gccagaactt cctgcataag caggattaat ggccatccgc gtaaacacat
     1021 tatgggtaaa ctgaggctct tgctgggcta atgttttacc cccactaaaa accagtaaga
     1081 gtgcaatatt cacccatgtt gtgcttttca ttagattcat ttccgcatcc ccactgaaat
     1141 cgggcataaa ataacacata aaatttcgac tatacaataa atcttctaaa atttcatccc
     1201 tttctccgat aacaactttc ccgatcgatt cttcattatc aagttattat acatttcatt
     1261 ccgttcgatc aaaaaggtaa aatcgttcaa ctgaggtttg aaaacttcgg tacaccacaa
     1321 ttaactacaa atatagtcta tttattgtaa accaaactaa tttttagccc cttgaaggaa
     1381 aaatttttga tatttttatc gaagggctca tacaaacatt gaacaaatac gtactatttc
     1441 ggagttcgaa agatatgttc tctttagttt aggttttgta attttgcaaa atattcaata
     1501 tttcaaagat ttagatactt tttatgcaaa aaagaagggc aatcgccaag gaaatcattg
     1561 ttagttatgg ccttattgtg atcggagcct ttatcatggc ggccggtttc gttctattta
     1621 tcaatcctta caagatagtt ccgggaggag tatatgggat tggtattgtg gtgcattata
     1681 ttaccaaagg attgattcca ttctggcccg aagggattcc cattgggcta gttggattaa
     1741 gtctagatat acctcttatt cttttaggaa tgcatttgct cggaacaaaa tttggtacga
     1801 aaaccattct gggctctttt ttgatgtcgt catttatgat ggttttgact tattttatag
     1861 gagaacacga ccctttaggc ttgcaggaag atattctgtt gagctgtatt tttggaggac
     1921 tcactgtagg attaggctta ggattgattt tcaggtcgag agcgacttca ggaggatcgg
     1981 atattattgc catgattctt tcaaaatatt caaaacttcc tgtaggacaa ttgcttatat
     2041 tggttgactc taccattgtc ctgataggtt tggcaacttt taaggattgg cgtattccat
     2101 tatattcttg gatagtaatt tatatcactg gtaaagtgat tgacctggtt gtggaagggg
     2161 taaatttcga gaaatcggta ttcattattt ccgatcaccc cgaagccatc cggcaaaaga
     2221 ttatttatga cctgagaaga gggggtactt ttattgaagg cgagggaatg tacaatggca
     2281 aaccccgtag aattatccat acagttatgt cgagacgtga ggtttcgctt ttactcgatc
     2341 atgttcggag aatcgaccct gatgccttta ttaccgtaac tgatgccagt gagatcctgg
     2401 gtgaaggatt caaatcttta aacgaataga acaattataa ttcccgaatt tctatggcct
     2461 ttatatctgt cgaaacattt cccagcaaac gatggttcgc ggcttatggc aagatccttt
     2521 tagggtcgtt gcttgtggcg tttggatatg ttttgttcat cactccttat aaaatcgtgc
     2581 ccgggggtgt ctatggaata agtattatca ttcactattt attgggcact cctgtgggtt
     2641 taactgctct ggcttttaat atccccatta ctctccttgc cacgaaagtt ttaggcccca
     2701 gatatggagc aaaaacagtt accggattca ttctgacttc tctgtttatt gatggattaa
     2761 cttattttct tggtaatgat cctctgaaaa ttagcgacga aatgcttatg tcgtcgttat
     2821 atggcggatt gttgatagga actggggttg gtttgatatt cagagccaga gcttcgtgtg
     2881 gaggcactga cgtgattgct atgatgattt cgaaattcat tcatcagccc gtggggcaaa
     2941 ttatgatcta tgtggattct ttgattgtac tgggaggttt tattgctttc aaagattgga
     3001 agattccact gtattctctg gtaactattt tcatggtggg caggatggtg gatgtcgtga
     3061 tggaaggatt gaattacaac aaatgtttgc tgattgtatc ggacaagcaa aaagaaattt
     3121 cgcaaagaat cattcatgat ttaaaaaggg gcggaactat tttccaatcg aagggaatct
     3181 atacgggtaa ggaacactct ttaatattca cagtgatgag ccgaagtgaa atgatcaaat
     3241 taaagtcgtt tattcgtcaa atcgacccta aggcttttat taccgtgatg gatacaagcg
     3301 aaattttagg caagggattc cgtcctctta acgagaatat agattaatga tactttttca
     3361 ggcctttagg cttttgttga tttaaaaaat gattcgatgc taggatgatt ctccgaatat
     3421 ataagattaa gatcaagaca tagcatggca atccgactcg attccaatgg aaactatgtt
     3481 ctttgcatgt gttgacttca ttctgaaatt tttcgacttg gcagttctta aaataatcat
     3541 ggaggaatgc cgtttcttga tttattctat atagattact cattctatcg atgtgtttcc
     3601 gaatagtgaa tgtcaataat tttatagttt tgccatataa tatcatttat ttatgaaaac
     3661 tatccgtaag atactgtgtt tttcggtttt attattgata ggttcaagca tttatgctca
     3721 ggataatgaa ccgggaggct ttcgaaaatt ttcgattggt tttgatttct ttaccgattt
     3781 ttggcaaaat ccgccttcag tgcttaatgc aaaatggtac aaccgtggtt ccaatgttaa
     3841 catcctcatc aataacaaga tgggtgaaag ccgctttgat ttttcttatg gagtgagctt
     3901 tggatcgcat aatttatata cggatgcatg gattaaagat gtgaaccaaa agagtgtatt
     3961 tgaaaagatt cccgatactc tcgatcataa aaaatacaag attacacttg cttattgcga
     4021 cttccctctc gagtttaagt acagggctaa aaataaatgg cgttattatg ccggcatcaa
     4081 gctgggtttt ttaatcaacc atcattcgaa atacattggc ctcgatcccg aaacgtatgc
     4141 ctacaatgtg aaaattaaaa gggatgatgt acgttatgta aatttctgga gatatgttgt
     4201 ttatgcccgt gtcgggtata aatggttcaa tgtatttgga caatattcct tgaatcatac
     4261 cttcaaagat gggcaaggcc ctgatatgta tccaatatcg gtgggaataa gccttatgcc
     4321 attttaaaaa gcgaagaaaa aacatcaggt aagtacttta gacaggctaa agcaataaca
     4381 aaaccattta tccatcccag aattacttgc atgaaagtat gtgccttgag ccgccagcga
     4441 gcccatccta aaaggatgcc tataagcaat aaaattagaa ctatccgggg aaaaatatca
     4501 tgtcgatgcc acgaaaaaaa tattattatt gaggataatc cacccaaact ggtcatgtga
     4561 atactgattt tccaaaattt attgactgcc caatctataa ggatactgac gattaaaatc
     4621 agaggtaatg tcagaagaag aggatt
//
LOCUS       DAORPD010000021         4604 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_3310_length_4604_cov_2.512420, whole genome shotgun sequence.
ACCESSION   DAORPD010000021 DAORPD010000000
VERSION     DAORPD010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4604
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3310_length_4604_cov_2.512420"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="PK976_00515"
     CDS             <1..228
                     /locus_tag="PK976_00515"
                     /inference="COORDINATES: protein motif:HMM:NF012479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66402.1"
                     /translation="YLKILRFYSFYPVKVKSKCGLTPQYEKLQQFHDNYHDKNFQIVA
                     FPVNNFLNQESGTESEIRNFCTTNNDGVILR"
     gene            646..2040
                     /locus_tag="PK976_00520"
     CDS             646..2040
                     /locus_tag="PK976_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HRC66403.1"
                     /translation="MLKSQIYSSRRKALRELMGNGIAFLPGNTEAPINYPANTYHFRQ
                     DSNFLYFFGLNQADLVGMIDLDKGIDILFGDEPSIDDIIWMGTQPSMKERASLVDVSD
                     TRSLSQLSTYLSDAIRAGRKIHYVPPYRAETAHWISVLLGIKPMFLKSYASLELTKAI
                     IQLREIKKEEEIEEIEKMIGVARLMHTTAMRMAKPGIIERQIFGRMEGIAFENANGTS
                     FPTILSINGQTLHNHYYGNVLTEGRLIVSDAGCESEDGYCSDITRSTPVGGKFNSRQK
                     EIYEIVLNANLKAIETAKPNIFYRDVHWEASRIIAAGLKELGLMKGDVDEAVATGAHA
                     LFFPHGLGHQMGLDVHDMEGLGEDLVGYDAEISRSIQFGLGLLRMAKRLKPGHIVTDE
                     PGIYFIPALIQQWKAQNKFDEFIDYQKVETYLDFGGIRIEDDILITENGCRVLGPFIP
                     KTVKEVEEEAAKSF"
     gene            2356..3570
                     /locus_tag="PK976_00525"
     CDS             2356..3570
                     /locus_tag="PK976_00525"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66404.1"
                     /translation="MYFRDIPGMDVLKGKLIESVNNKRISHTQLFFGPEGSASLGLAI
                     AYGRYIQCRQPIHNDACGKCPSCLKYNKLQHPDLHFIFPVAKKGKSSDTEEDEIVGKT
                     GDEPSSLDFMREWREFILSNKALVSYKEWLDFAGLGKRALIYTRDTREIFKRLNYKSY
                     ESEYKILIIWYPEKFFHSASHRLLKALEEPPEKTVFILVSENKDEILPTILSRCQSTF
                     VPPFTQNELKQALQSNLHLPEDQALHYASVADGNFIVARQLANENSDFHNYFDFFANW
                     FRYCYNLKYKPKKSEAKEAPYFVDALKIFESLGQEGQKQFLRFGIQLLRNSLFFSMDT
                     SQLVKLEGEEQLFARNFSPFIHSRNIALYSDAFGKAIMALERNSSPNMVFTNLTMSIH
                     QLITLAKAKSVS"
     gene            3659..>4604
                     /gene="ricT"
                     /locus_tag="PK976_00530"
     CDS             3659..>4604
                     /gene="ricT"
                     /locus_tag="PK976_00530"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HRC66405.1"
                     /translation="MTKKIDINNPLFSRGCTCLPTYAEQNVYSNGCAKFSTDDYLSEI
                     PPSGYETDFEFAEIRLKNNHKEFMHVPSELKLHQGDIVVVDGNPGHDIGIISLTGHTA
                     RLQMKAKNIDPERTELKKVFRLARPADIEKWIAVSSREDKTMLQTRGIVEDLNLSMKV
                     NDVEYQGDGSKAIFYYTADDRVDFRELIKILADRFKIRVEMRQIGIRQEASKVGGIGP
                     CGRELCCSIWINRFHTVSTQAAKIQQLAPNPQKLTGQCGKLKCCLNYEYEAYMDALKE
                     FPDMELVLKTQKGEAVCQKIDVFKKLMWYAYVKDPNNFL"
BASE COUNT         1426 a          872 c          984 g         1322 t
ORIGIN      
        1 tatttgaaga ttttaagatt ttattcgttt taccctgtaa aggtaaagag taaatgcgga
       61 cttactccac aatacgagaa gcttcaacaa ttccacgata attaccacga taagaacttc
      121 caaatagtcg ctttcccagt caataacttc ttaaatcaag aatctggaac cgaaagtgag
      181 attaggaatt tttgtactac taataatgat ggtgtaattt tacgatgatg tggaggattt
      241 tgatcaaagg gagagaacat gcattcaatc tattgctggt tgaatggtaa atcagacaat
      301 ggggtcatgg atttggaagc aaaatagaat tttaagaaat gccttatcga tgaaaatggc
      361 cgtttattaa agatgttgcc gccatatact tcgcctctcg atgactaaat cattcgatgg
      421 attactaacg ctgctgaata agaaatatag gatgacgtca taccattggc gatagaaggt
      481 ggtgtttttt aacttaaggt cgtcagtatt tggataattt gccaaacaga aatcttactt
      541 tgagagaaag atttaattta gcgtcatcaa ttttacttgt gaaaataagt tttaaataat
      601 gcaggctcaa ataatctttc tataaatcgt aaaaaccata gttctatgtt aaaatcacag
      661 atttattcca gtcgtcgcaa ggctttgcgt gaattgatgg ggaatgggat agcattcctt
      721 ccgggcaata ctgaagcacc aataaattat cccgccaata cgtatcattt caggcaggat
      781 agtaacttcc tctatttttt tggcttgaat caggccgacc ttgtggggat gatcgatctc
      841 gacaaaggaa tagatattct ttttggcgat gaacccagta ttgacgatat tatatggatg
      901 gggacccagc cttcgatgaa ggaaagagct tctctggtag atgtttctga tacacgctct
      961 ctgagccaat tgtctacata tctatcggat gctatacgag caggaaggaa aattcattat
     1021 gttcctcctt atcgtgccga gactgctcac tggatttccg ttctattggg catcaaacca
     1081 atgttcctaa agtcttatgc ttcattggag ctaacgaagg ctatcatcca actgcgcgaa
     1141 ataaagaaag aagaagagat tgaagaaatc gaaaagatga taggcgttgc ccgcttgatg
     1201 catacgacgg ctatgcgcat ggccaagcct ggcatcatcg aaaggcagat ctttgggaga
     1261 atggaaggca ttgctttcga gaatgctaat gggacgagct ttcctaccat tttatccatc
     1321 aatggacaaa cgcttcataa tcattattac ggcaatgttc ttaccgaagg tcgcttgatt
     1381 gtttccgatg ccggatgtga atcggaagat ggttattgca gtgatattac ccgttctact
     1441 ccagttggtg gaaagttcaa cagccgacaa aaggagattt atgagattgt gttgaatgct
     1501 aatttaaaag ccatagaaac agcaaaaccc aatatttttt accgtgatgt tcattgggaa
     1561 gcatctcgaa tcattgcagc aggcctaaag gaattgggtt tgatgaaagg agatgtggat
     1621 gaggccgtag caactggtgc tcatgctttg ttttttccgc atggccttgg tcatcagatg
     1681 ggtctcgatg tacatgacat ggaaggttta ggagaagacc tggtgggata cgatgcggag
     1741 attagccgta gcattcaatt tggattgggt ttactacgaa tggcaaaacg tttgaaacct
     1801 ggccatatag ttaccgatga gcctggaatt tactttattc cagcactcat tcagcaatgg
     1861 aaggctcaaa ataagttcga cgaatttatt gactaccaaa aggttgaaac ttatctcgat
     1921 tttggaggca ttcgcattga agacgatatc ttaattaccg aaaatggttg tcgggtttta
     1981 ggccctttca ttcccaagac ggtaaaagaa gtggaagaag aggcagcaaa atcgttttga
     2041 gagacataga acgaatagga ttttaggagc ttacgatttt ttcatcatag ggtaaaatta
     2101 aaaagccttg gatagcgcca ataaactcca ttaatatttc atgtttcttt tgtcgattac
     2161 aatccacaaa aaaatcttta tgaatcggaa tatgagtatt ccgagggcgt agtgataaaa
     2221 taaaaacgca cactaaatac tctcatcaga gcactcaatt gccaaaagtc agatttgttg
     2281 agttaaagaa gaaagaatgt ggggataaat cagtttggaa tatctttgcc ttgaaatcgt
     2341 atattaaaaa atcgaatgta tttcagggat attccgggaa tggatgtgct caagggaaag
     2401 ctcatcgaaa gtgtaaacaa taaacgtata agccataccc aacttttctt cgggcccgaa
     2461 ggttcggcaa gtctgggact ggccatagct tatggcagat atattcaatg caggcaacct
     2521 atccacaatg acgcatgcgg caaatgcccc tcctgtttga aatacaataa attacagcat
     2581 cccgatttgc attttatctt tccggtagcc aaaaagggca aatcatccga tacagaagaa
     2641 gatgaaatcg tcgggaaaac tggagatgag ccttcaagcc tggattttat gagggaatgg
     2701 agagaattta tcctctccaa caaggcactt gtaagttata aggaatggct cgattttgca
     2761 ggcttaggaa agcgtgcact gatttataca cgtgatacaa gggagatatt caaacggctt
     2821 aattacaaga gttacgaatc agaatacaag atattgatta tctggtatcc cgagaaattt
     2881 tttcactctg cttctcaccg cctgctcaag gcactcgaag agcctcccga gaaaacagtt
     2941 tttattttgg tttccgagaa taaagatgaa atccttccca ctattctttc gcgctgtcag
     3001 tccacttttg tccctccatt tactcagaat gagctcaaac aggcgttgca gagcaatctt
     3061 catctacccg aagaccaagc cctgcattat gcctcggtgg cagatggcaa cttcatagtc
     3121 gcacggcagc ttgccaacga aaacagcgat ttccataact atttcgattt tttcgccaat
     3181 tggttccgct attgctacaa tctcaaatac aaacctaaaa aatcggaagc aaaagaagct
     3241 ccttatttcg tagatgcctt aaaaatcttc gaatccttgg gccaagaggg gcaaaaacaa
     3301 tttctgcgct ttggaattca attgttacgc aatagcttgt tctttagcat ggataccagt
     3361 caactcgtca aactcgaggg tgaggaacaa ttgtttgccc gtaatttttc gcctttcatt
     3421 cattctcgaa acattgcttt atactccgat gcttttggaa aagccatcat ggctcttgaa
     3481 cgaaacagca gtccaaatat ggtttttacc aatctcacta tgagcattca tcagctcata
     3541 actttggcaa aggcaaaatc ggtgagttag ttttatgtaa ttttgtaaaa tatttctttg
     3601 cgccttcaaa tgtgtaaatc tattttttga aatgatttcg taatagcctt gtttgccgat
     3661 gactaaaaaa attgatataa acaatccatt attttcgaga ggttgcactt gccttccaac
     3721 ctatgccgag caaaacgttt attctaatgg ttgcgcaaag ttttcgaccg acgattatct
     3781 ttccgaaata cccccttcgg gatatgaaac agatttcgaa tttgccgaga ttcgcttgaa
     3841 aaataatcac aaagaattta tgcatgttcc atccgaacta aagcttcatc agggcgatat
     3901 agtggtggta gatggcaatc ctggccatga tatcggaata attagtctta caggccatac
     3961 ggctcgtctg cagatgaaag ctaaaaatat agatcctgaa cgtacggaat tgaaaaaagt
     4021 atttcgtttg gccaggccgg ccgatatcga gaaatggata gcagtgagct cacgtgaaga
     4081 taaaactatg ttacagacac gaggcatagt cgaggatctg aatctctcga tgaaggtaaa
     4141 tgatgtagaa tatcagggtg atgggagtaa agccatattt tattatacag ccgatgatag
     4201 agtcgatttc cgtgaactca taaaaattct tgccgatcgt ttcaagatta gggtagaaat
     4261 gcgccagata ggtataaggc aggaagccag caaagtgggt ggcattggcc cttgtggccg
     4321 tgaactttgc tgctctatct ggataaatcg tttccatacc gtgtcaaccc aggcggctaa
     4381 aattcaacaa cttgcaccca atcctcaaaa acttactggc cagtgtggaa agctcaaatg
     4441 ctgcctgaat tatgaatatg aggcttatat ggatgccttg aaggaatttc ccgatatgga
     4501 actcgttctg aaaacccaaa aaggtgaggc tgtttgccag aaaatcgatg tctttaaaaa
     4561 actaatgtgg tatgcttacg tgaaagaccc caataacttc ctcg
//
LOCUS       DAORPD010000022         4508 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_3385_length_4508_cov_2.374579, whole genome shotgun sequence.
ACCESSION   DAORPD010000022 DAORPD010000000
VERSION     DAORPD010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4508
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3385_length_4508_cov_2.374579"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="PK976_00535"
     CDS             <1..400
                     /locus_tag="PK976_00535"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HRC66406.1"
                     /translation="EPSLYQKLELPAQKLGSAFQKVNFLRDIKSDTQNLGRYYFPEMS
                     TMSLNQQSKKIIEHSIEQDFKVAFPGIRQLPGRSKMAVAIAYYYYNSLFIKIIHSSPE
                     KILSQRIRISNIKKYLIMVKVCILYFFKRI"
     gene            411..968
                     /gene="idi"
                     /locus_tag="PK976_00540"
     CDS             411..968
                     /gene="idi"
                     /locus_tag="PK976_00540"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF002995.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HRC66407.1"
                     /translation="MSDKVILVNINDKIIGEIAKIEAHEKALLHRAVSVIIFNSKGQM
                     LLQKRALNKYHSAGLWSNTACTHPFPGEDNKTAAQRRLKEEMGIHAEVFKIFDFIYHE
                     KLDNGLTEHEFDHVYIGISDEPPQPDPNEVSDFDYFERNFLKNELSSSPKHYTVWFAI
                     LFDRVYDFIDQQHLRERLITYNQTS"
     gene            1179..3500
                     /locus_tag="PK976_00545"
     CDS             1179..3500
                     /locus_tag="PK976_00545"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRC66408.1"
                     /translation="MRKFIVLSSCCMIWGVFIFSGLKSWAQEASIRGVIYEKESGEPA
                     IFVNVFLGKTTLGSATDVNGYYIINNIPAGSYNLMVTGIGYDSLVVPITIRKGEIITR
                     KLYISKKSIALEGVSISAEREAARTETRTSVVKVTPRQIKQIPSIGGQPDLAQYLQVL
                     PGVVFTGDQGGQLYIRGGAPIQNKLLLDGMVIYNPFHSIGLFSVFDTDILRNADIYTG
                     GFGAEFGGRISSVMDLTTRDGNKKRLSGKIDASTFGAKLLLEGPISKQKEDNSGSSSF
                     ILSVKNSYLEETSKTLYKYVNEDGLPFNFFDIYGKVSFNADNGSKVNLFGFNFTDRVN
                     NYKALSDFHWDAIGGGTNFVVIPGKTPVLLEGVISYSNYKVSLKETGKNDRTSQIGGF
                     NAGLNFTYFINKDELRYGFEFQGFNTDYYFFNSLNRVIQQKENTTEIGPYVKYKMHRG
                     KFLIEPGLRIQWYTSLSNISPEPRLAIKYNVNDRLRIKGAGGFYSQNLISATSDRDVV
                     NLFYGFLSGPDNLPEEFNGEKVTDKLQKAWHTIGGIELDLSNNVTMNIEAYYKKFTQL
                     TNINRNKIFDDTEIFADQPDELKNDFIIETGDAKGLDISIKFENDRFYFWGVYSLGYV
                     SRFDGNNTYSPHFDRRHTINLVAAVYFGSARDWELSSRWNFGSGFPFTQSQGYFEKIT
                     FENGIYSNPATENGNLAILYAELNTGRLPVYHRLDIDLKHKWELSQHSHLQLNIGATN
                     IYDRENIFYFDRIAFERVYQLPVMYNVGFTFSF"
     gene            complement(3507..4352)
                     /locus_tag="PK976_00550"
     CDS             complement(3507..4352)
                     /locus_tag="PK976_00550"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRC66409.1"
                     /translation="MIYKKISDYNVPAVGLGTWEMGGGEQADYSHDRHWITIIRSAIE
                     NGLTHIDTAEMYGNGHTEELVGEAIKGFPRQNLFITTKVWLTHLRSNDLFSSFEKSLR
                     QLQTDYIDLYLVHHPNPDIPLEETLMAMNRLVEQKRVRAIGVSNFDISLIKEAVRISS
                     VPISAVQIEYSLLARNSGIYTQNQEKEIIPYCQENQIAVIAWRPLGKTNYIRLATNDV
                     IKKIAEKYHATPAQIALIWLIAQKNILAIPKTGSLEHLIENSKASDIDLSMEDGKLLR
                     EAFEE"
BASE COUNT         1394 a          931 c          830 g         1353 t
ORIGIN      
        1 tgaaccgagc ctttatcaaa aactcgaact gcctgcacaa aaattagggt cggcttttca
       61 aaaggtaaac ttccttcgcg atattaaatc agatactcaa aacttaggca gatactactt
      121 tccagagatg tctaccatga gcctgaacca acaaagcaaa aaaataatcg aacattctat
      181 cgaacaagac ttcaaagtgg ccttccctgg cattcgacaa ctacctggtc gttcaaaaat
      241 ggctgtggcc attgcatatt actattataa cagccttttt ataaaaatta ttcactcttc
      301 acccgaaaaa attctctcac aaagaataag gatttcaaac atcaaaaagt atttaataat
      361 ggtcaaagtt tgtattttgt attttttcaa acgtatctaa tctatcaatt atgagcgata
      421 aagtgatttt agtaaatata aacgataaaa ttattggaga aattgccaag attgaagcac
      481 atgaaaaggc gttattgcac agagcggttt ccgtgataat cttcaattcg aaaggccaga
      541 tgcttttaca gaaacgagca ttgaacaaat atcattcggc tggtttatgg tcaaatactg
      601 catgtacaca ccctttccct ggcgaagaca acaaaacagc agcacaacgc aggctgaaag
      661 aagaaatggg aatacatgcc gaggttttta aaatattcga tttcatttat catgaaaaac
      721 tcgataatgg tttgactgaa catgagtttg atcatgttta tataggaatc tctgacgaac
      781 caccacaacc cgatcccaat gaagtaagtg attttgacta ttttgaacgt aatttcctga
      841 aaaatgagct atcttcttct cctaagcact atacagtatg gtttgcaata ctttttgaca
      901 gggtatatga ttttattgat cagcaacatt taagggaaag gcttataact tacaatcaga
      961 cttcataatg attgagctat acatagacaa attttctctt ctactttaaa ccctatcttt
     1021 cattatgtat ctatttcata tacttttagt tttgctctta tgcaggcgat gtttttgcct
     1081 catttatcaa gccattcatc atatccccaa ttggggaagt atgactcttt ttataccttt
     1141 gtgatccaat tttacttacg tcagataata atgatattat gaggaaattt atcgttcttt
     1201 cgtcctgttg catgatttgg ggtgtgttta tattttcggg attaaaatca tgggcacaag
     1261 aagcaagcat tagaggagta atttacgaga aagaaagtgg cgaacccgcc atttttgtta
     1321 atgtttttct cggcaaaaca acccttggtt cggctaccga tgtaaatggt tattacatca
     1381 tcaacaatat tcctgccggc tcgtataatc tcatggtaac aggcatagga tacgatagtt
     1441 tggtggttcc gattaccata agaaaagggg aaataatcac acgaaaactt tatatttcca
     1501 aaaagtccat agctttggaa ggggtgagta tttcggcaga gagggaagct gcgcgaaccg
     1561 aaactcgcac ctcggtggtt aaggttactc cacgccagat taagcaaatt ccaagtatag
     1621 gaggccaacc cgaccttgca caatatctac aggtcttacc cggggttgta ttcaccggcg
     1681 accagggagg tcagctgtat attcgtgggg gtgcacctat acaaaacaaa ctccttctcg
     1741 atggaatggt catctataat ccctttcatt ccatcggttt gttctcggta ttcgacaccg
     1801 atattcttcg caatgccgac atttacactg gaggattcgg ggcagaattt ggcggcagaa
     1861 tttcttccgt catggacctc accacccgcg atggcaataa aaagcgcttg agtggaaaaa
     1921 tcgatgcctc tacctttgga gcaaaactgc tgctcgaagg acctatcagc aaacaaaaag
     1981 aagacaattc gggaagttcg tcctttattc tttcggttaa aaattcctat ctcgaagaaa
     2041 cttctaaaac cctttacaaa tacgtaaatg aagatggact tccatttaac ttcttcgata
     2101 tttatggcaa agtatctttc aatgccgaca acggaagcaa agtgaacctt tttggtttta
     2161 atttcaccga tcgggtaaac aattataaag ccttatctga ttttcattgg gacgccattg
     2221 gtggaggcac aaacttcgtg gtaattcccg gaaaaactcc cgtactcctc gaaggagtta
     2281 tttcttattc taattataag gtaagcctca aagaaaccgg taaaaacgat cgtacaagcc
     2341 agataggcgg tttcaatgcg ggtttgaatt tcacctattt tatcaacaag gatgaattac
     2401 gttacggctt cgaatttcaa ggtttcaaca ccgattatta tttcttcaat tccctcaacc
     2461 gggtcattca acaaaaggaa aatacaaccg agattggccc ttacgtaaaa tataagatgc
     2521 atcgcggaaa atttctcatc gaacccggat tgagaataca atggtatact tctttaagca
     2581 acatatcgcc tgaaccacgt ttggccatca aatataatgt gaatgatcgc ttgagaatta
     2641 aaggcgcagg aggcttctat tcccagaacc tgatcagtgc tacaagtgat cgagatgtag
     2701 tgaatctctt ctatggattt ctttcaggcc ccgacaacct ccccgaagaa ttcaatgggg
     2761 aaaaggtcac cgataaattg caaaaagcat ggcataccat aggaggtata gaactcgacc
     2821 tatccaataa tgtaaccatg aatatagagg catactataa aaagtttacc caactcacca
     2881 atatcaaccg caataagatt tttgacgata cagaaatatt tgccgatcag cccgatgaat
     2941 tgaagaatga tttcatcatc gaaaccggag atgctaaagg tttggatatt tcgataaaat
     3001 tcgaaaacga cagattctat ttctggggag tttattcatt gggatatgta agtcgattcg
     3061 atggaaacaa tacctattct ccacatttcg acaggcggca taccataaat ctggtcgccg
     3121 ctgtatattt cgggtcagca cgcgactggg aacttagctc tcgctggaac ttcggctcgg
     3181 gattcccctt cactcagagc caaggttatt tcgaaaaaat aactttcgaa aatggaatct
     3241 attccaaccc ggcaaccgaa aacggaaatt tagccatcct atatgccgaa ttgaacactg
     3301 gccgcttacc cgtttatcat cgcctcgaca ttgatttgaa acataaatgg gaactcagcc
     3361 aacattctca tttgcaactg aatattggag ctacaaatat ctacgaccgc gagaatatct
     3421 tctatttcga ccgtattgcc tttgaacgtg tctatcaatt gccagtcatg tataacgttg
     3481 gcttcacctt ttctttctaa tctattctat tcctcgaatg cctcacgtaa tagctttcca
     3541 tcttccatcg agagatcgat gtcggaagct ttcgaatttt ctattaaatg ttctaatgaa
     3601 ccagtttttg gaatagctaa tatatttttt tgtgcaatga gccaaatgag tgcaatttga
     3661 gcaggagtag catgatactt ttcagcaatt ttttttatta cgtcattggt tgccaatcgg
     3721 atataattgg tttttcccaa tggacgccat gcaattaccg caatttgatt ttcctgacaa
     3781 tagggaataa tttctttctc ctgattttga gtataaatcc cagaatttct cgctaaaagg
     3841 ctatattcta tctgtactgc cgaaattgga actgaggaaa ttctcaccgc ttcttttatc
     3901 aaggagatat cgaaattact cacccctatg gctcttactc gcttctgctc aaccagccta
     3961 ttcatggcca tcagggtttc ctccaaagga atatcaggat tgggatgatg aacaagatag
     4021 agatcgatat aatctgtctg aagctgacga agacttttct cgaatgagga gaataaatca
     4081 ttggaacgaa gatgagtaag ccagactttg gtagtaataa acaggttctg acgtggaaac
     4141 ccttttatgg cttcccctac aagctcctcc gtatgaccat tgccatacat ttcagctgtg
     4201 tcgatatgtg taaggccatt ttcaatagcg cttcgaataa tagttatcca atgcctgtcg
     4261 tgagaatagt cggcctgttc gccacctccc atctcccaag ttcctaaacc gactgccgga
     4321 acgttataat ctgaaatctt tttgtaaatc aatgctttaa aaacggttag attttataaa
     4381 atcaagcaac taaaaataaa actttacccg catctggtat aatcttcaat cctccatcat
     4441 gtatttcgta aaattcctaa tttcgttata cataattact tgttttaatc atgtcttcat
     4501 aggctttc
//
LOCUS       DAORPD010000023         4491 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_3400_length_4491_cov_2.126240, whole genome shotgun sequence.
ACCESSION   DAORPD010000023 DAORPD010000000
VERSION     DAORPD010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4491
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3400_length_4491_cov_2.126240"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="PK976_00555"
     CDS             <1..188
                     /locus_tag="PK976_00555"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295947.1"
                     /note="Catalyzes the hydrolysis of AMP to form adenine and
                     ribose 5-phosphate using water as the nucleophile; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HRC66410.1"
                     /translation="STGALLLVSDQPMIPDGVKTEEKDREVSLHFTELHLRIGIDSLR
                     QLINNGLTVKHLIFDLE"
     gene            213..1226
                     /gene="holA"
                     /locus_tag="PK976_00560"
     CDS             213..1226
                     /gene="holA"
                     /locus_tag="PK976_00560"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HRC66411.1"
                     /translation="MTFEQIYTDLKNKIYKPLYLFYGDESFYIDFLTDYMTGNILKEE
                     EKDFNLSVFYGLNTTGSSIVDRARRYPTMADYNLVIVREAQQLRSELGQLSQYALHPS
                     SSTILTVALKLQSSRKHELLKDFKKAVDKFGVSFESKKLYDDKIPQWIRKYLSARGYT
                     ITDHACQMMADDLGNDLSLIVNECAKLIINLPKGSNITPEIIEKYIGISKDFNPFELC
                     RTISQRNKFGTAQIVLYFASNPKENPPIKISTVLFNYFQKLWIYHQLNDKKASVAAPI
                     LGVNPFFVKEYAAAARNFNLPQCSQVLQLISEYDLKFKGVDIDQADPVEMMKEMIYKI
                     LSV"
     gene            complement(1223..>4491)
                     /locus_tag="PK976_00565"
     CDS             complement(1223..>4491)
                     /locus_tag="PK976_00565"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HRC66412.1"
                     /translation="VLITDLKLMALPNGQYEYRGNLATDNFDLGRILEVPQLIGAVSM
                     AGDVEGKGFSLDSANVRLNVMVQKFEFNRYPYKNLTVRGELSKKQFQGEINIDDPNLN
                     LNFDGSIDLSEDIPEYNFTAGVSHAYLQNLGMIDRGDSLCELNFELRANFLGSHLDNL
                     EGSIDLTEVNYTEKGKDYSANRIFLNTLLTPTGERTLSLLSPFFDASVRGEIQFAQLG
                     YSFSLFFNNYLNSFHNLGQTLSDTTGQNFVYDIRIKKLNELTALFLPWLKVANNSRIK
                     GQFDTKDRILSLEAQTDTLSVYGVKMNNLSLRSQSFQDAITLETNIRKVNFVEMKKDS
                     ILYFGLDKFDLTANIAYDSIRYNMKWGLLPEDSSNYGNLKGYLSFSNYPEIFMQFIES
                     SFNLDDNIYVIQPDNKIIFRKQEIDIHRLEITSENESLYIDGAYSSDSTKMLNAQFHN
                     FSLSHADFFLQPFGLQIGGLLQGHIQLHQENNKPLIQAEMRIDHLAVNQQNLGFMELT
                     SLWNTKSKALFVKAKSTLVNNEFQSHPIDLSGYYYPSDPKQNFDIKLKTENFNLKPIQ
                     PFLNMAIAELEGFLSSNIELEGSVKSPEIHGKLKFIRAKIRPKFFNTTYSFSDEIIVE
                     PSRIIFNKLKINDSLGNVAFCDGYIAHKNFQDMYLDIRLQPENFILFNAEPQQNKYVN
                     GIAFISGQIRVSGSINNLNIEANAITEKGSNIYVPLSSTVDVRSSSFLYFKQPEDTLK
                     LKKVERTNLNVSGFNVFSVLTLTPQARIKVSLPQDAGTIEGTGEGMITLEVNSRGDLN
                     INGQYEIQSGSFLFNLQNILSRMLEIEKGSTIHFSGDPLDAQINLKASFTTRTTLSGL
                     GLDLDSTVTSARIPVKTIIHLQNKLLNPFITFSIQFPKIDPDIERAVYAKLDTTNQMV
                     MTQQFVSLLVLNNFSFAVTNKSFGNSLGISSFQMLSNQISNLLSQISQDFDIGINYRP
                     GDAISAQELELALSTQLFDQRVTIDGNLGVVGMQNANKTSNVVGDINVEVMLTRDGKL
                     RLRAFNRSNTTELLTVSAPYTQGIGLFYRKDFNNLAELFRKKRKIIQEESLNISTSAN
                     "
BASE COUNT         1366 a          883 c          869 g         1373 t
ORIGIN      
        1 tttctacagg cgccttactt ttggtatccg atcaacccat gattcccgat ggagtgaaaa
       61 ccgaagaaaa agaccgggaa gtttctcttc attttaccga acttcacctt cgcattggga
      121 tagattcact acgccagctc atcaataatg gtctcacggt gaaacatctg atcttcgacc
      181 tcgaataaat tctccccgat tttctattca aaatgacttt tgaacaaatc tataccgatt
      241 taaaaaacaa gatttataaa cccctttatc tattttacgg ggatgaatcc ttttacatcg
      301 attttctgac cgactacatg acagggaata ttctcaagga agaagaaaaa gatttcaatc
      361 tttcggtatt ctatggttta aatactactg gttcgtcaat cgtagacagg gctcgccgtt
      421 atccaaccat ggccgattac aacctcgtta ttgtgcgaga agcgcagcaa ttacgctcgg
      481 aactgggcca gctaagccag tatgcccttc atccaagctc ttctacaatt ttgacagttg
      541 ccttgaaatt gcaatccagc aggaaacatg aattgttgaa agatttcaaa aaagccgtcg
      601 ataaattcgg agtatccttt gaatcgaaaa aactttacga tgataagatt ccacaatgga
      661 ttcgaaaata tctgagtgcc aggggttata ctattacaga tcatgcctgc cagatgatgg
      721 ccgacgacct tggcaacgac ttgagcttaa tcgttaacga atgcgccaaa ctcatcatca
      781 atctgcccaa agggtcaaat ataacccccg agattatcga aaaatacatt ggcattagca
      841 aggatttcaa tcccttcgaa ttatgcagga ctatttcaca acgcaacaaa ttcggcactg
      901 ctcaaatcgt tctttatttt gccagcaatc caaaagaaaa tcctcctata aaaatcagta
      961 cagttttatt taattatttc caaaaattat ggatttatca tcaattaaat gataaaaaag
     1021 cctcagtagc tgctcctatc ctgggagtaa atcctttttt cgtgaaagaa tatgctgcag
     1081 ccgcgcgaaa tttcaatctc cctcaatgca gtcaggtttt gcaacttata agcgaatatg
     1141 atttgaaatt caagggtgta gatatcgatc aggctgatcc ggtggaaatg atgaaggaaa
     1201 tgatctataa gattctatcg gtttaattgg cagaggtaga gatattcaga gattcttcct
     1261 gaatgatttt acgttttttc ctgaacagtt cggccagatt attaaaatct tttctgtaaa
     1321 acagtccaat gccttgggta taaggggccg aaacggtaag taattcagta gtattggatc
     1381 ggttgaaggc tctgagacga agtttaccat cgcgtgtgag cattacttct acattgatat
     1441 cacccaccac attgcttgtt ttgtttgcat tttgcattcc caccacgccc aaattcccgt
     1501 cgatagttac acgttgatca aaaagttggg tgcttagggc aagttcgagt tcctgagccg
     1561 aaatagcatc gccaggccga taatttatcc cgatatcgaa atcttgacta atttgagaca
     1621 atagattgct gatttgattc gaaagcatct ggaacgaaga gattcccaag ctatttccaa
     1681 atgatttatt ggtaactgcg aagctaaaat tattcaacac cagaagggat acgaactgtt
     1741 gcgtcataac catctgattg gtagtatcga gtttggcata aacggctcgt tcgatgtcag
     1801 gatcgatttt tggaaattga atgctgaagg tgataaaagg attcaacagc ttattttgaa
     1861 gatgtataat ggtttttaca ggtattcgag ccgaagtgac ggtagaatcg aggtctaatc
     1921 ccaatccact aagggtagta cgcgtggtaa atgaagcttt taaatttatt tgagcatcca
     1981 acggatcgcc agaaaaatgt atggtcgaac ccttctcgat ttcgagcatt cggctgagga
     2041 tattttgcag attaaacagg aatgaacctg attgaatttc atattggcca ttgatattga
     2101 gatcgcctct cgaatttact tctaacgtaa tcataccttc gcccgtcccc tctatggttc
     2161 cggcatcttg tggaaggctt acttttatcc tggcctgagg agttagtgtc aataccgaaa
     2221 aaacattgaa accagaaaca ttcaaattgg tacgttccac cttcttcaac ttcaaagtat
     2281 cttcgggttg tttaaaatat aagaaactcg aagaacgaac atccaccgtc gacgacaatg
     2341 gcacgtaaat attgcttccc ttctcggtaa tggcattggc ttcgatattc aagttattta
     2401 tagaacccga aacgcgaatt tgacccgaaa taaaggcaat tccattcaca tatttgtttt
     2461 gttgaggctc ggcattgaac aaaatgaaat tttcaggttg caatcgaata tccaaataca
     2521 tatcctggaa attcttatgg gcgatatatc catcacaaaa agcaacattc cccaacgaat
     2581 catttatttt cagtttgttg aaaattattc tcgaaggttc gactataatt tcatccgaaa
     2641 aagaataggt agtattgaaa aatttcggac gaatcttggc acgaataaac ttcaactttc
     2701 catgaatttc aggactttta actgagcctt cgagttcgat attcgagctt agaaaacctt
     2761 cgagttcagc gatagccatg tttagaaaag gttgaatggg cttcagattg aaattttccg
     2821 ttttcagttt gatgtcgaaa ttctgttttg gatctgaagg ataataatat ccggataaat
     2881 cgatgggatg agattgaaat tcattgttaa ccagagttga tttagccttt acaaagaggg
     2941 ctttcgattt tgtattccac aaggaagtga gttccataaa acccagattt tgttgattga
     3001 cagccagatg atcgattcgc atctcggctt gaatcaaagg tttgttattt tcctgatgaa
     3061 gctggatgtg tccttgaagc aagcctccta tttgtaaacc aaagggctgc aaaaagaagt
     3121 cggcatgaga aagactgaaa ttatggaatt gtgcattaag cattttagtg ctatcggagc
     3181 tgtaggctcc atcgatgtat aaactctcgt tttctgaagt gatctcgagt cgatggatat
     3241 cgatttcctg tttccgaaag attattttat tgtcgggttg aattacgtag atattatcat
     3301 ctaaattgaa cgaagattct atgaattgca taaaaatttc aggataattc gaaaacgaca
     3361 ggtaaccttt caggtttcca taattggatg aatcttcggg taagaggccc catttcatat
     3421 tgtatcggat actatcgtat gcaatattgg cagtaaggtc aaatttatcg aggccaaaat
     3481 acaagatact atccttcttc atttctacaa aatttacttt tctaatattg gtttccaatg
     3541 taatagcatc ttgaaaggat tggctgcgca aggaaagatt attcatcttg acgccgtaaa
     3601 cgctcaaggt atcggtttgt gcttcgaggc ttaaaatccg gtctttagtg tcgaattgtc
     3661 ctttgatgcg gctattattc gcaactttta gccatggcag gaatagtgcg gtcaattcgt
     3721 tcagcttttt tattcttata tcatagacga aattctggcc tgtggtgtcg ctaagggttt
     3781 gacccaaatt atgaaatgaa ttcaaataat tattaaaaaa taacgaaaat gaatatccta
     3841 attgggcaaa ttgaatttct cctctcacac tggcatcgaa gaatggagaa aggaggctca
     3901 aagtacgttc accagtcgga gtaagtaacg tatttaaaaa aatgcgattg gccgaataat
     3961 ctttcccttt ttcggtataa ttcacttcgg ttaaatctat agaaccttcc agattgtcga
     4021 ggtgtgagcc caagaaattt gccctgagct cgaaatttag ttcacataac gaatcgccgc
     4081 gatcgatcat tccaagattt tgcaaatacg catgcgaaac ccctgcagta aagttatatt
     4141 cgggtatatc ttcgctcaag tcgatcgacc catcgaaatt gagattcaag ttgggatcat
     4201 cgatgttgat ttcaccctga aattgctttt tggatagttc tccacgcacg gtcagatttt
     4261 tataaggata acgattgaat tcgaattttt gaaccataac attcaatctt acatttgcgc
     4321 tatcgagact gaaacccttg ccctcgacat ctccagccat acttaccgcc cctattaatt
     4381 gcggaacttc taaaatcctt cccagatcaa aattatctgt cgccaaattt ccacggtatt
     4441 catattgacc atttggaagt gccattagtt tcaaatcagt aatcaacaca c
//
LOCUS       DAORPD010000024         4489 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_3401_length_4489_cov_2.425124, whole genome shotgun sequence.
ACCESSION   DAORPD010000024 DAORPD010000000
VERSION     DAORPD010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4489
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3401_length_4489_cov_2.425124"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            312..941
                     /locus_tag="PK976_00570"
     CDS             312..941
                     /locus_tag="PK976_00570"
                     /inference="COORDINATES: protein motif:HMM:NF021759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HRC66413.1"
                     /translation="MTIRERSSIEKNLAPLLPEAALHQVVDLLFRFQVDLVVTYPRSG
                     RLGDYLYNTKNNRHRISININLNRYQFLITLLHEFAHLLVQERFKTVVRPHGKEWHAA
                     FCEISMPFLRDDIFPLDVKAAFGAHLKSRYGSAYADKKLSKILERYNGIEQNRYKVEL
                     GTLPVSSKFFLSGRSFQCISRQGNVILCQELKTGTIYRMSSSVFVKPYL"
     gene            996..2090
                     /locus_tag="PK976_00575"
     CDS             996..2090
                     /locus_tag="PK976_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HRC66414.1"
                     /translation="MNPNDYCVIMAGGIGTRFWPISRADHPKQFIDILGTGQTLIQQT
                     FQRFKPLCLTQNIFVVTNEEYFSMVREQLPELPPENILCEPSGRNTAPCIAFASYHIA
                     SINPSANIVVAPSDHIIQDMQRFHEVIGTALRTVREHNWLLTLGIQPSRPDTGYGYIQ
                     FVDEIIPGVDSRIKKVKTFTEKPNLELARAFLASGDFLWNAGIFIWNLQTILQALEAY
                     LPEVASLFNEGKEIFNTPEEKAFIEHVYTHCKSISIDYGVMEKADNVYTLMSDFGWSD
                     LGTWASLYEVRQKDSDGNSIVGNQVMSYDTHNCIIHVPDEKLVVIQGLNNCIVVEDEG
                     MLLICNKNDEQQIRSIVNDLKSRKYINLKR"
     gene            2106..4265
                     /locus_tag="PK976_00580"
     CDS             2106..4265
                     /locus_tag="PK976_00580"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HRC66415.1"
                     /translation="MIFIKKLYLTLIACCVFCTGLYADEGMWLPYLLDQLQIVRMQSM
                     GLKLSAEDIYSINHSSLKDAIVIFGRGCTGEVVSADGLVFTNHHCGFGAIQSSSSVEN
                     DYLKEGFWARSYKEEIPISGLTVQFLMFVQDVTHDMLEGVDEEMPETQRDSVLKINEK
                     HILEAIPAQDGLGGVVKEFYAGNQFYLFVYQSYKDIRLVGAPPTAIGKFGGDTDNWMW
                     PRHTGDFSVFRIYTSPSGKPTTYQVNNIPLKTNRFLTINAGGIKEGDFTMIMGYPGRT
                     DRYITSWGVDQALNITNPSIVKIRDKKLEIIRNSMATSDEIRIKYAAKYASISNYWKY
                     YIGQNKGLKRLKIYEKKKQQEKEFAEWIAADTYRFNRYGEVLDSFEQAYRLLNQTAKP
                     SVYIREAAFGPEIIAFASRFASLASTEMKDSKDINAANLHTNAKGMGDSFFKNFDLAT
                     DKKLFAAMMNMYYNDIDPGFIPNILSNANKKYKGQWDKYADEVYEKSMFSSMEAFSKF
                     LLNPSASSLKDDPVYQLALAFREISRKIEAMNIQAKDQLKRSKRLYMEGIQQMGAGNP
                     FYPDANQTMRLTYGQVLDYQAADAIEYSYFTTHFGILEKEEPSNPDFQIPQRLKEVFN
                     RKDFSPYYKNDTVVACFLTNNDITGGNSGSPVLNGKGELIGLAFDGNWEAMSGDIAYE
                     PELQRTINVDIRYVLFLIDKYARSNHIMRELKIEYER"
BASE COUNT         1381 a          810 c          978 g         1320 t
ORIGIN      
        1 gaccacctgc cagaagatga aatttcatcc agcgattaat tcttgcgatc ataataattt
       61 tcggcgtttg tggttaatta aatggaaatt tactataaag gaaatatttt tcatgaagaa
      121 accgttgcgg aattatttga aaacacatac aatagtatta gggctaaggt ttttaggtat
      181 gatttttctt ttcgtatggg ttgtttcttg ccagacttcg agcaaggccc agcacagaaa
      241 gacaaaaaag aaatcaggag attgcgactg cccgtcgttt actcaagtga gagttgtaaa
      301 cccaatgatt tatgacgata agggaacgga gttcaatcga gaaaaatctg gcgcctttat
      361 tgcctgaagc tgcccttcat caggttgttg atctattgtt ccgttttcag gtcgatttgg
      421 tggtaacata tccccgcagt ggaagattgg gcgattattt atacaataca aagaataaca
      481 ggcatcgcat cagcataaat atcaatctca accgctatca gtttttaatc actcttttac
      541 atgaatttgc ccatttgctg gttcaggaaa gattcaaaac agtggtaaga cctcacggga
      601 aggaatggca tgctgctttc tgcgaaattt cgatgccttt tcttcgtgat gatattttcc
      661 ccttagatgt caaggcagct ttcggtgcac atttgaagag ccgttatggt tccgcttatg
      721 ccgataaaaa attgagtaaa attcttgaaa ggtataatgg aatcgaacaa aatcgctata
      781 aggtcgaact gggtactttg ccggtttcaa gcaaattttt tctctctggg aggtcgtttc
      841 aatgtataag ccgtcagggc aatgttatac tttgtcaaga acttaaaaca ggaacgattt
      901 ataggatgag ttcttccgtt tttgtaaaac cttatttgta gaaaaatgaa atggttacat
      961 ttgcacaaac agccaaaaaa taaaattatt gcgaaatgaa tccaaatgat tattgtgtaa
     1021 ttatggcagg tggcattgga acgcgtttct ggcccataag tcgtgccgat catcccaagc
     1081 aattcataga tattcttgga actgggcaaa cacttatcca gcaaactttc caaaggttta
     1141 agcccttatg cttaacgcaa aatatttttg tggtcacgaa cgaagaatat ttttcaatgg
     1201 taagggaaca acttccagaa ctacctcccg agaatatttt gtgcgaacca tcgggacgca
     1261 ataccgctcc atgtattgct tttgccagtt atcatattgc aagtatcaat ccctcggcta
     1321 atattgtggt tgctccttcc gatcatatta ttcaagatat gcaaaggttt cacgaagtca
     1381 ttggaactgc attaaggact gtaagagaac acaactggct tcttacgctg ggtattcagc
     1441 caagcaggcc cgatacgggc tatggttata ttcaatttgt tgatgaaatt attcccggag
     1501 tagattctcg tatcaagaaa gttaaaacct ttaccgaaaa acccaatctt gaacttgcaa
     1561 gagctttcct tgccagcggt gattttctgt ggaatgcagg catctttatc tggaacctgc
     1621 agacgattct ccaagccctc gaagcttatc tgcccgaagt tgcctcccta ttcaacgagg
     1681 ggaaagagat tttcaatacc cccgaagaaa aagcattcat cgaacatgtt tatacccatt
     1741 gcaaaagcat ctccattgat tatggcgtaa tggaaaaagc cgataacgtt tatacgctga
     1801 tgtcggattt cggctggtct gatttgggaa cctgggcttc attgtatgaa gtacgtcaga
     1861 aagattcaga tggaaactcc atcgttggaa atcaagttat gagctacgat acacataatt
     1921 gcatcattca tgtaccagat gaaaagcttg tagtaataca gggattgaac aattgtattg
     1981 tggtggaaga cgaaggaatg ttgctgattt gcaataagaa tgatgaacag cagatccgta
     2041 gcatagtgaa tgatttaaaa tcgagaaaat atattaatct taaacggtaa tcaaagatct
     2101 tgaatatgat ttttattaaa aaactgtatt taaccctgat agcttgttgt gttttttgta
     2161 ctggccttta tgctgatgaa ggaatgtggc ttccttattt actcgatcag cttcagattg
     2221 tacgcatgca atccatgggg cttaaacttt cggccgaaga tatttacagc attaaccatt
     2281 cgagcctgaa ggatgccata gtaatttttg gccgtggatg cacgggcgaa gtcgtatcgg
     2341 cagatggttt ggtttttacc aatcatcatt gtggctttgg ggcgattcag agttcgagtt
     2401 ccgttgaaaa cgattatttg aaggaaggtt tctgggcaag aagttacaag gaagaaatcc
     2461 caatatctgg tctcacggtc caatttctta tgtttgttca ggatgttaca catgatatgc
     2521 tcgaaggagt tgatgaagaa atgcccgaaa ctcagcgtga ttccgttttg aaaatcaatg
     2581 agaaacatat tctcgaggct attccggctc aggacgggtt gggtggagtt gtgaaagagt
     2641 tttatgccgg gaatcagttt tatctttttg tatatcaatc ttataaagat attcgcctgg
     2701 tgggagctcc tcctactgca ataggaaaat ttggaggaga taccgataac tggatgtggc
     2761 ctcggcatac gggcgatttt tccgtcttta gaatttatac tagcccaagc ggaaaaccta
     2821 ctacctatca ggtaaataat attcctctga aaaccaatcg attccttact attaatgccg
     2881 gagggattaa agaaggagat tttaccatga ttatgggcta tccaggtcgt accgaccgat
     2941 acattacttc ttggggagtg gatcaggctt tgaatatcac aaatccttcc atagtaaaaa
     3001 tacgcgacaa gaaactcgaa atcattcgaa acagcatggc aacgagcgat gaaatcagaa
     3061 taaaatatgc tgccaaatac gctagcattt ctaattattg gaaatattac atcggccaaa
     3121 acaagggtct gaaacgatta aaaatctatg aaaagaaaaa acaacaggaa aaggaatttg
     3181 ccgagtggat agcggccgat acttaccgat ttaacagata tggtgaagtt ctcgatagct
     3241 ttgaacaggc ctacagacta ctcaaccaaa cggcaaaacc tagcgtttac atcagagagg
     3301 ctgcctttgg ccctgaaatc attgcctttg cttctcgatt cgcatccctt gcttctactg
     3361 agatgaaaga tagtaaagac attaacgcag ccaatcttca tactaacgcc aagggaatgg
     3421 gtgattcttt cttcaaaaac ttcgaccttg caacggataa gaaacttttt gctgctatga
     3481 tgaatatgta ttataatgac atagaccctg gtttcattcc gaacattctt tcgaatgcca
     3541 ataaaaaata taaaggccag tgggacaaat atgcagatga agtgtatgaa aaatcgatgt
     3601 tttcgagtat ggaagctttt tcgaaatttc tgttaaatcc ctcagcttca tcccttaagg
     3661 atgatcctgt ctatcagctt gccctggctt ttagagaaat cagcaggaaa atcgaagcaa
     3721 tgaacattca ggctaaagat caattaaaaa gaagcaaacg gctatatatg gaaggcattc
     3781 agcaaatggg agctggtaat ccgttctatc ccgatgccaa tcaaaccatg cgtcttacct
     3841 atggtcaggt attggattat caggcggccg atgctatcga gtatagttat tttacgacac
     3901 atttcgggat tttggaaaaa gaagaacctt cgaatcctga ttttcaaatt cctcaacgat
     3961 tgaaagaggt ttttaaccgt aaggactttt cgccttatta taaaaacgat actgttgttg
     4021 cttgttttct tacaaataat gatattacgg gtggaaacag tgggagtcca gttttaaatg
     4081 ggaaaggaga attaattggg ctggcattcg atgggaattg ggaggctatg agcggtgata
     4141 ttgcttatga acctgaactt caacgtacca ttaatgtgga tattcgctac gtattgtttc
     4201 ttatcgataa atatgcccgg agtaatcata taatgcgtga gctgaaaatt gagtacgagc
     4261 ggtaagccaa gtcgataaat tacaatggtg tgattttttt atcatctttg ctaatttgtg
     4321 agatggggga atggtgattc ccgaaaagaa aaaagtatgg cgggataaga gttctgtgat
     4381 caggtgatat atagtgcaag atggatggtg attaaaaatg gatggtgatt aaaagaggaa
     4441 agccggcaat aatgactaac ttatttcgga cgccgagagg gatgcctgg
//
LOCUS       DAORPD010000025         4436 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_3450_length_4436_cov_4.403333, whole genome shotgun sequence.
ACCESSION   DAORPD010000025 DAORPD010000000
VERSION     DAORPD010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4436
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3450_length_4436_cov_4.403333"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            346..1110
                     /locus_tag="PK976_00585"
     CDS             346..1110
                     /locus_tag="PK976_00585"
                     /inference="COORDINATES: protein motif:HMM:NF023521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3575 domain-containing protein"
                     /protein_id="HRC66416.1"
                     /translation="MKRSIVLGLTIFLSFSLFSQNSTSLKPDYLSYGLLDGQTIVKFN
                     LLGAAVRNYSFYAERILSKRVSVLLSYNVMPKGGVPYIGQFTDDESAKSIRLGTFSFT
                     PEVRFYLASGYGGGFYIAPYYRYERFTLYQLSTTFTDDNDDERTIDVDGSLNTHSGGI
                     LLGCQWLVGSKKNIVLDWSILGVHGGKSSGDFTGITDETISPEDQQTLKQDIEDGLED
                     IPMIKSEVSVDEHSANIRIDGPWAFIRTSFSIGYRF"
     gene            1377..2906
                     /locus_tag="PK976_00590"
     CDS             1377..2906
                     /locus_tag="PK976_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HRC66417.1"
                     /translation="MKYNSQAKQLRLDIFSGTLEKSLDPSNRWYKLANLMPWDKIERI
                     YNKSLRNQHSGGGNKPARMVIGALIIKHKMNLSDEETIRLIAENPYMQYFIGLEEFTS
                     KPVFDSSLFVYVRKRMNVESINEMSLAMMEAEWGKGVESEGGGGNESDNADKKKDEQK
                     NEDDSFVDEEGMLHKGSIKIDATCCDVEVRYPTDLDLLNDAREVSERLIDKLCRLSGS
                     PKPRTHRKRARHHYLQVIKKKRKSKQLIRKGIKQQLGYLCRNLRSITDIVSKGSTSMF
                     DSLTKKEKQWIGTIIKVYHQQKGMYDTHTHQCPDRIISVFQPHVRPIVRGKSKSKVEF
                     GSKIGASVVGGYTFIDHFSWDAYNESEDMERHIKAYYERFGYLPTTCYADKIYMNREN
                     RALLKRYHIRAAGKPLGRPSKEMQTEVCRREAAKDEGKRNEVESTFGTDKRVYRVNNV
                     RAKLPDTADVWTAACFFVKNVMKFLKGLLFVLFEKMLLEVKKKFSAINRELLFSLKTV
                     A"
     gene            complement(3483..>4436)
                     /locus_tag="PK976_00595"
     CDS             complement(3483..>4436)
                     /locus_tag="PK976_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HRC66418.1"
                     /translation="CGDHAILSCLYKAMAELNIEPHQTAVISGIGCSSRLTYYMNAYG
                     IHGIHGRALAIATGVKVANPKLSVWVATGDGDSLAIGGNHFIHAIRRNVDINVLLFNN
                     KIYGLTKGQYSPTSDRGFVSKSSPFGTVEDPFIPAELCFGARGNFFGRAIDVDLKNTT
                     DLMVAAARHKGTSIVEILQNCVIFNNGIHNKISDKTFRSEKTILLKHGEKMIFGANND
                     KGIVAEGWNLKAVTIGQDGYTLDDILVHNATTVDSTLHLKLAMMGYENDLPVALGVIR
                     SVGAPTFEAEIEKQIAEVQQKKPKQTFTDFLLHSSNVWEVK"
BASE COUNT         1416 a          831 c          946 g         1243 t
ORIGIN      
        1 taaataaaca acaaaaaagt ataaaaacaa aaaacactca aaaaaatcaa attccaagat
       61 atttaaatta aaataaacat aataaaagat taaatataaa attatatgaa aataacgaat
      121 taatcctaaa aataataata agaaaaaaaa taaaaataaa actataataa acataaaatt
      181 caggtctgaa atttccccaa gttcgacgaa agaaatattt gtaatacgtt aattattaag
      241 tatcatactt atttctactc tatgcgataa ataaatgcta atttattttt ttgttgagaa
      301 aaattatata aatttgcttc ggattaaaac taaattcgag gccttatgaa aagaagtatt
      361 gtcctggggc taacgatatt tctgtcgttt tccctgtttt cgcagaacag cacgtcactc
      421 aaacctgatt atttgtctta cggtcttttg gatggccaaa ctatcgttaa gttcaatttg
      481 ttgggagcgg ccgttagaaa ttattccttt tatgccgaac gaatcctcag taaaagagtt
      541 agtgtgcttt tgagttacaa tgtcatgcca aagggaggag tgccctatat cgggcaattc
      601 accgatgatg agtctgcaaa aagcattcga ttgggaacat tttcgtttac tcctgaagtt
      661 cgtttttatt tggcttcggg ctatgggggc ggattttata tagcgcctta ttaccgatat
      721 gagcgattta cgctttatca actttcgacg acttttaccg atgacaatga tgacgaacgc
      781 actattgatg tggatggctc actcaatacc catagtggtg ggatcctttt gggatgtcaa
      841 tggttggtgg gctccaaaaa gaacattgtc ctggattggt ctattctggg cgttcatggc
      901 gggaaaagtt cgggtgattt tacgggcata acggacgaaa ctatttcgcc agaagatcaa
      961 caaacactaa agcaggacat tgaagatggg ctggaagata ttccaatgat taaaagcgag
     1021 gtaagcgtag acgaacattc tgccaatatc aggatcgatg gtccgtgggc ttttattcga
     1081 acttcgtttt cgatcggata cagattctga aatttgcttt aaaaatagaa aaaatgggta
     1141 tcgcttgatt atggtactca ttttataaaa cagattctaa tgaagcgtta tatatacagt
     1201 tacagaaatg aggtcattaa aacagttgta gctatttttt ttgttgttat cataatctca
     1261 ttaataatta gggtctcctg aattcggtca atcaattgat atttaacaaa ataagttcgt
     1321 attttcttgt tattttcggg aaaaagtcgt acatttatgg gcaaaacggc tacattatga
     1381 aatacaactc gcaagcaaaa caactacgac ttgatatttt ttcgggcaca ctcgagaaat
     1441 cgctcgatcc atccaaccgg tggtataaat tggcgaacct gatgccttgg gacaagattg
     1501 aacgcatcta caataagagt ctgcgcaacc aacatagcgg aggaggcaac aaacctgccc
     1561 gcatggtaat tggagcattg atcatcaagc acaagatgaa tctttcggac gaagaaacca
     1621 tccggctcat tgccgagaat ccttacatgc agtattttat cggattggaa gagtttacga
     1681 gtaaaccggt attcgattcg agtttattcg tttacgttcg caaacgcatg aatgtcgaat
     1741 caatcaacga aatgtcgtta gccatgatgg aagcagaatg gggaaaaggc gtagaatcgg
     1801 aaggaggcgg aggaaatgaa agcgataatg cggataagaa gaaagacgaa cagaagaatg
     1861 aagatgacag ttttgtcgat gaagaaggca tgcttcacaa agggagcata aagatagatg
     1921 ccacgtgttg cgatgtggaa gtcaggtatc ccacggatct tgacctgctc aacgatgccc
     1981 gagaagtcag tgaacgtttg atagacaagc tgtgccgctt aagtggcagt ccaaaaccac
     2041 gaacacatcg caaaagagca agacaccact atcttcaggt aatcaagaaa aagaggaagt
     2101 cgaaacaact catccgaaag ggaatcaaac agcaattggg ttatctgtgt cgtaaccttc
     2161 gaagcataac cgatatcgta tcgaaaggct ccacctccat gttcgattcg ctgacgaaaa
     2221 aagagaagca atggatagga acgatcatca aggtttatca tcaacagaaa gggatgtatg
     2281 atactcacac ccatcagtgc cccgacagga ttatcagtgt gtttcaaccc catgttcgcc
     2341 ctattgtcag agggaaatcc aaatcaaaag tagagttcgg ttcaaagata ggagccagcg
     2401 tggttggcgg ttacactttc atcgatcact tcagttggga tgcctataac gagtcagagg
     2461 atatggagcg gcacatcaag gcctattatg aacgtttcgg atatcttccg actacctgtt
     2521 atgccgacaa gatttacatg aacagggaaa atcgggcgct tttgaagcgt taccacataa
     2581 gagcagccgg caaaccgttg ggcaggcctt ccaaagagat gcagacagaa gtctgccgga
     2641 gggaagcagc caaagacgag gggaaaagga atgaagttga atctaccttt ggaacggaca
     2701 aaagagtcta tcgggtaaac aatgtgcggg ccaaacttcc cgatactgcc gacgtgtgga
     2761 cggcagcatg tttctttgtg aagaatgtta tgaagttcct caaagggctt ctttttgtct
     2821 tattcgaaaa gatgctgttg gaggttaaga aaaaattttc tgccataaat cgggaacttc
     2881 tcttctccct aaaaactgtg gcttgatata tatgaattta aacggaatta ttcagtgaac
     2941 cctaattaaa acgtaagcct gataaattta gtttatcgac ttgctaactg gtgtcatgta
     3001 tcattttttt tgtgcgggga aagtgtatcg ctttccccgt ttcaattgtg ctttcgaagg
     3061 ggattttccg ggcatgtttc gcaatcattc caaccataaa cgacctctta tcatattaac
     3121 tatttatttg ttttagttat atatattaat aaattggtgg gacacgaccc aagtacgacc
     3181 catgtacgac gcaaatacgg acttggtgtg tatgtactca gcatggagat taccgaaaaa
     3241 tcataggttt aataaagata tatttacaat ataggcgaat ttataactta attttatatt
     3301 gttatcgggc aaatggaccg gctcttttca aagaatatga tgagacgctg tctcctgatt
     3361 gcgaaacaga atagggttac aatttttacc aaaacaggat ttgctgcgaa aaagggaaaa
     3421 aagaaaagag gtcgtctcaa aagtgaggca acctctttat cattactaca gtatatcata
     3481 tttcatttca cttcccacac attcgacgaa tgcaacaaaa aatcggtaaa cgtttgtttc
     3541 ggtttttttt gttgtacttc ggcaatctgc ttttcgattt cagcttcgaa agttggagca
     3601 ccaacagaac ggattacgcc cagagccaca ggaagatcat tttcataacc catcatggca
     3661 agttttagat gtaatgtgct atctacagtt gtggcattgt gtaccaaaat gtcatccaat
     3721 gtatatccgt cctgtccgat cgtaacggct ttcaaattcc atccttcggc aacgattcct
     3781 ttatcgttat tagcaccaaa aatcattttt tcgccatgtt tcaacaaaat ggttttttcg
     3841 gagcgaaatg ttttatctga aatcttatta tggataccat tattgaagat gacacagttt
     3901 tgaagaatct ctacgatgga tgttcctttg tgtcgagctg cggcgaccat caaatcggtt
     3961 gtgtttttca gatcgacgtc gatggctctt ccaaagaaat tgccacgcgc accaaaacaa
     4021 agttcggcag gaataaaagg gtcttcaaca gttccgaaag gtgaggattt cgaaacaaat
     4081 ccgcggtcgg aagtaggaga gtattgtccc ttcgtcagtc cgtagatttt gttgttgaac
     4141 aacaatacat tgatgtccac attacgacga atggcatgaa taaaatgatt tcccccgatt
     4201 gccaaagaat cgccatcgcc ggtggctacc caaacactca gtttgggatt ggcaactttc
     4261 actccggtag cgatagccaa agcgcggcca tgaatgccat gaatgccgta agcgttcata
     4321 taataggtca atcgtgatga gcaaccaata cctgaaatga cggccgtttg gtgaggttcg
     4381 atatttaatt cggccatcgc tttgtagaga caactcaaga tggcatgatc accaca
//
LOCUS       DAORPD010000026         4392 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_3502_length_4392_cov_2.198985, whole genome shotgun sequence.
ACCESSION   DAORPD010000026 DAORPD010000000
VERSION     DAORPD010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4392
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3502_length_4392_cov_2.198985"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            428..913
                     /locus_tag="PK976_00600"
     CDS             428..913
                     /locus_tag="PK976_00600"
                     /inference="COORDINATES: protein motif:HMM:NF022479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HRC66419.1"
                     /translation="MISIDSVKLIETMLAPGIMISACGLLLLGIHNKYSIVVNRIRVL
                     EEEKRSLIPGFVEKTLNIYQSKRLESIQSQIVRFEYRVKLIRNVVFFYTLSVALFVLS
                     SLSIGLSYIIESRWAESFSMAFFLAGMISVLIGIIFAAREVWKGYEIVQIEVQESRIP
                     E"
     gene            922..2262
                     /locus_tag="PK976_00605"
     CDS             922..2262
                     /locus_tag="PK976_00605"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRC66420.1"
                     /translation="MEQQDTIIHNGLLLTMDREARILPHHSIRIIDGIIQEILPVESS
                     KDDHGIRVINASGSLVMPGFINCHTHAAMTLLRGFADDKPLHEWLSDYIWPAEAVFMN
                     RENIALGTRLAILEMIASGTVMFSDMYFFEDEVARACEEAGMRVLLGEGLLGNHTPHF
                     TTPYQGIDFTETLAEQYKNHSLIEVAYAPHAVYTCPREILSEVALRSEKNHLPVHIHL
                     AETAEEVENCIASTGKHPVDYLDEIGLLDQRLIAAHVVHVEEEHLKLLTHKGVRIAHN
                     PSSNLKLNSGFPPIHKQIQYKIPLGLGTDGAGSNNNLSIPVEMRHTALVDRLLHQNRE
                     SDQAFQIVKMATIGGAKVLGADQITGSLEAGKAADLIIIPLDKPHLQPIYNIYSTLVF
                     SLHSSDIDTVMINGKIVMKDRKILTLDEESLLKDLKRVAGDIREKFKMKNHLAY"
     gene            2268..3563
                     /gene="rimO"
                     /locus_tag="PK976_00610"
     CDS             2268..3563
                     /gene="rimO"
                     /locus_tag="PK976_00610"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HRC66421.1"
                     /translation="MESKGIKIYTLGCYKNQVDSEKLAGKIESAGINAMHTDKFEGNT
                     LIINTCGFIKDAKEESIEVIMQAIEAKKRGRIGRIVVMGCLSERYKKELREEIPEVDA
                     WFGVESIEQIIQWLNLPEYRERRIIRKLANPPHYAYLKIAEGCSRRCSFCAIPNIRGS
                     HRSRPIDEIMEEAEGLASKGVKEIILISQDLTSYGIDLYKKQMITQLVKHLSESNLFP
                     WIRLHYLFPTSFPEDLLHLMATKDNICPYIDIPLQHISERILKSMHRGIDKASTMALI
                     EKFRTLVPHAALRSTFIVGYPGETEKEFDELLNFINEVRFERLGVFTYSEEEGTPAAL
                     LKDDVNEFTKKRRAELLMEIQSEISFEKNESLIGKTMKVLIDRKEEKYFIGRTIYDSP
                     EVDNEVIIDATHTSLQVGQFYPIIIEKADLYETFGKPYK"
     gene            3579..4313
                     /locus_tag="PK976_00615"
     CDS             3579..4313
                     /locus_tag="PK976_00615"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HRC66422.1"
                     /translation="MKHLRTFIFISVICLSMKPVFAQTYDSIKNTRDDKGSKQGYWEE
                     NINRQLEKGWYVDNKREGVWRTYYPKGFLHEVIEYKDGKRGGLRLEIDSRGYLAVEEY
                     FENDQLHGRSINYSMGAHPRVEQWYKNGQPDGLKKIYYDNGKVQEESNYKNGKRHGIS
                     RWYNPKGQVIAEFNYFEGEFDGVQKTYNDAGILIKSEEYSKGVLHGNYSEWDDAGNLI
                     ISGQYKNGEKVGKWKEYKGEKEEEKE"
BASE COUNT         1400 a          854 c          950 g         1188 t
ORIGIN      
        1 cgacagatct gttatctcga tacaccccga tggagtactt gacgatcgat ggataatagg
       61 tggccaaaca gggctgagct tgtcgaagcc cgtatcgttg ccataataaa tcaatttcgt
      121 gtgctctgct attttggttt ctttcccttt ccctttctct atccccttcc ctatccctat
      181 ctcacttttc cctatttttt cttttatcta aaattcaagg atagaatgaa ttctttttat
      241 tatattttat tgtaatccaa attattacat catattattc tattgaccgt tgtcagaatc
      301 tccacactaa taataaattt gagttaaacc ttgtgatgga aggaaatttc gaaaggataa
      361 agatgactct ggttggttgt tatgttctga ctaattttgc accataatca ataaatctca
      421 agaaagaatg atatcaattg attctgttaa gcttatcgaa accatgctgg cgccgggtat
      481 tatgatttcg gcctgtggac tacttttatt aggcattcac aataaataca gcattgtagt
      541 aaatcgcatc cgggtactcg aagaagaaaa gcgaagcctc attcctggtt ttgtggaaaa
      601 aaccctgaat atataccaaa gcaagcgttt agaatccatt cagtcgcaaa ttgttcgttt
      661 cgaatacaga gtgaaactca ttcgcaatgt agtgtttttt tacaccttgt cagtagcctt
      721 gtttgtactg agttcattga gcattgggtt gagctatatt atcgagagca gatgggctga
      781 atccttttct atggctttct tcctggcagg tatgattagc gtattgatag gaattatttt
      841 tgctgcccgt gaagtatgga agggatatga aattgttcag atagaagtgc aggaaagtag
      901 aattccggaa taaacccaaa aatggaacaa caagatacaa tcatacacaa tgggcttctt
      961 ttgacaatgg atagagaagc tcggattttg ccccatcaca gcattagaat tatcgacgga
     1021 attatccaag aaattcttcc agtcgaaagt tcgaaagatg atcatggtat tcgagtaatc
     1081 aatgcttcgg gcagtttggt aatgcctgga ttcatcaatt gtcatactca tgcagccatg
     1141 actcttttgc gtggttttgc cgacgacaag cctttgcatg aatggttgag cgattatatc
     1201 tggccagccg aagccgtatt catgaatcgt gaaaatattg ctctgggaac aaggctggca
     1261 atcctcgaaa tgatagcctc cggaacggta atgttttccg atatgtattt cttcgaggac
     1321 gaagtagcca gagcttgtga agaagcaggt atgcgtgttt tattaggtga aggattattg
     1381 ggtaaccaca ccccgcattt taccactcct tatcagggaa tcgattttac tgaaacactg
     1441 gccgaacaat ataagaatca ttcactcatc gaggtagcct atgcacctca cgctgtttac
     1501 acctgtccaa gagaaattct gagcgaagtc gctctgcgtt cagaaaagaa tcatttaccg
     1561 gtacatattc atctggccga aaccgccgaa gaagtcgaaa actgcattgc atcgacgggg
     1621 aaacatcctg tggattatct cgatgaaata ggtttgctcg atcaaaggtt aatcgctgct
     1681 catgttgtcc acgtcgaaga agaacatttg aaacttctga ctcacaaggg agttcgtatt
     1741 gcacacaatc cctcgagcaa cctgaaacta aactcaggct tccctcctat tcacaaacaa
     1801 atacaatata aaattccttt aggactgggc accgatggag ctggttcgaa caataatctt
     1861 agcatcccgg tcgaaatgcg tcatactgcg ctggtagacc ggttgctcca tcaaaaccgc
     1921 gaatcagacc aggcattcca aatcgttaaa atggctacta ttggaggagc caaagtttta
     1981 ggagccgacc agattacagg atcgttagaa gctggcaagg cagccgattt gattatcata
     2041 ccactcgata aaccccatct tcagcccatc tacaacattt attctacact tgtttttagc
     2101 ttgcattcat ccgatatcga taccgtgatg atcaacggta aaattgtgat gaaagatcga
     2161 aaaattctca cgctcgacga ggaatcactg ttaaaagact taaagagggt agcaggagac
     2221 atcagggaaa aattcaaaat gaagaaccac ctcgcatatt gaaataaatg gaatcgaagg
     2281 gtataaagat ttatacttta ggatgttata agaatcaggt agatagtgaa aaacttgctg
     2341 gcaagataga atcggcagga ataaatgcaa tgcatactga taagttcgaa gggaatacat
     2401 tgattatcaa tacatgtgga tttattaaag atgcaaagga ggaatccata gaggtcatca
     2461 tgcaggccat agaggctaaa aagagaggca ggattgggcg aatcgtcgtt atgggttgcc
     2521 tgtcggagcg ctacaaaaag gagctacggg aagaaattcc cgaagtggat gcatggtttg
     2581 gtgtggaatc cattgaacag atcatacaat ggctcaattt gcctgaatat cgagaaagaa
     2641 gaattattcg caaattagcc aatcccccgc actatgccta tctgaaaata gcggaaggtt
     2701 gcagccggag atgttcattt tgtgccattc caaatattcg tggctcacat cgttcgcgcc
     2761 ccatcgacga aatcatggaa gaagccgaag ggctcgcctc aaagggagtt aaagaaatta
     2821 tcctgatctc tcaagatctc acttcgtatg gaatcgatct atataaaaag cagatgatta
     2881 ctcaactggt gaagcatctg agcgaaagca atctcttccc gtggatcagg cttcattatc
     2941 ttttcccgac ctcttttcca gaagacctct tgcatttaat ggcaacaaaa gacaacatct
     3001 gtccttatat cgatatcccc ctgcaacata tttcagaaag gattctgaaa tccatgcata
     3061 gaggaattga taaggcttct actatggctc tgatcgaaaa attcagaact ctcgtgcctc
     3121 atgccgcact tcgttcgaca ttcatcgttg gatatcctgg cgaaaccgaa aaagaattcg
     3181 acgaactttt aaactttatt aatgaagtca gattcgaacg cctgggtgtt ttcacctatt
     3241 cggaagagga aggaacaccg gcagccctcc tgaaagatga tgtgaacgaa tttactaaaa
     3301 aacggcgagc agaattactc atggaaattc aatcggaaat cagtttcgaa aaaaatgaat
     3361 cacttatcgg aaaaaccatg aaggtgctta ttgatagaaa agaagaaaaa tatttcatag
     3421 gacgtacaat ttatgattct cccgaggtgg ataatgaagt gataatagat gctacgcata
     3481 catcactaca agtcggccaa ttctatccga taatcatcga aaaagccgat ctttacgaaa
     3541 cttttggaaa accctataaa tgattaaacc attgcaatat gaaacactta agaactttta
     3601 ttttcatatc agtaatttgt ctgagcatga aacccgtttt tgcccaaacc tacgactcca
     3661 taaaaaacac tcgagacgat aaaggctcaa agcaagggta ttgggaagaa aacatcaatc
     3721 gccaactgga gaaaggatgg tacgtcgaca acaaaagaga gggcgtttgg agaacttatt
     3781 atccaaaggg atttttacac gaagtgatcg aatataaaga tgggaagcgc ggtggtttga
     3841 ggctcgaaat agatagccgt ggatatctgg cagtggaaga atacttcgaa aatgaccagc
     3901 ttcatggccg ttctatcaat tactccatgg gagctcatcc tcgggtagag caatggtata
     3961 agaatggaca gcccgatggc ttgaaaaaaa tttactacga caacggcaaa gtgcaggaag
     4021 aatcgaatta caaaaatggg aaacgtcatg gcatatcacg ctggtacaat cccaaggggc
     4081 aggtgatagc tgaattcaac tactttgaag gtgaattcga tggtgtgcaa aaaacttaca
     4141 acgatgcagg aatactcata aagtcggaag aatattccaa gggtgttctc catggaaatt
     4201 attcagaatg ggacgatgca gggaacctga ttatttcggg ccaatacaag aatggtgaaa
     4261 aagtagggaa atggaaagaa tacaagggag agaaggaaga ggaaaaggaa taaagaattt
     4321 tggccgcttg tgcatggagc tcatcattca ttttactcac attgtggctt gtatttgatg
     4381 ggtgtggagc ag
//
LOCUS       DAORPD010000027         4370 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_3519_length_4370_cov_2.101738, whole genome shotgun sequence.
ACCESSION   DAORPD010000027 DAORPD010000000
VERSION     DAORPD010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4370
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3519_length_4370_cov_2.101738"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2220
                     /locus_tag="PK976_00620"
     CDS             <1..2220
                     /locus_tag="PK976_00620"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC66423.1"
                     /translation="NSDESQWRTANYIDGEGQLQPLNGFVWDVKVGSNGIVVASIESK
                     VYVSELGQPNGFINQSTKQVLPDTVLNPEKLPSIGLGRIELAVAPSNPDYIYACASDP
                     YGYFDNIYRSIDKGITWEIIFPGHSSLLPDIFVVNNVAYGLVANTIVVYPNNPEEILV
                     GGINMWHGKFINEGYYYWGTDAISSYATSKFSPLYLHELHHTYVFKPNDPKTFLIGTD
                     GGISTNIDGELYFKSLNRTYMTSQFVTLNVNGFGNPIGGTLGNGIQYIGDGTNSPIGA
                     VEVWNGDFNNSGEGGYVAISKINPKVFILSLKGGPFRRTEDKGFSFSTTFLNSGMSAP
                     ANTYCPMILWESFNDPTSMDSTRFKASKDITEGEEIIVRSQIYDYPFKTIAPHDIHKG
                     DVVYFKDPLQAKTFLALGDKVYYTKEILDFTKEPEWWQVAKVVGTVSCMSLSADANHL
                     YVGTESGNVFRISNLTHANSAATASITSATCVVATQQIKSFPNRIVTSIAVDPKNPQH
                     ILVTLGNYGFDEYVYNVTNALDSLPKFTSVQTNLPHGPVYSSLIELNNSNLIILGTEY
                     GIFLTNNISAPNWVNDNGPMGRIPVKVLLQQTVNGGGIFVPSDDPNIPGVYYPEITNY
                     GVIYAATYGRGVYSSNFFVGIDEPNIDTPPTNAQLAIYPNPVRNELSFTLKGDYASEV
                     FIEIYDFSGRKSKENHLKIQPGSNTFTMDVSNLKRGTYILRCISDTSISTGKFIIVK"
     gene            2252..3730
                     /locus_tag="PK976_00625"
     CDS             2252..3730
                     /locus_tag="PK976_00625"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC66424.1"
                     /translation="MKRIFTFFFLLIGFSVIAQPKIYTPTLLLPNNGATNQMPDVTLS
                     WSAVTGLGVIKYKLEIDDNPDFTSPKVFYTEFLTGYTMSNLHYGVQYHWRVKAYDLAT
                     NDSSYWSATRNFTVFSKVDNKKPENKTKEITPLATLEWKDRLGPNLVTGNTYFNYILD
                     TVETFDSPMAYLGTVSGSVYKATTPKMRFGTRYFWKVRAINEKDTSNWSDAWYFRTLD
                     TIVLKDPANNATNQQINLTLSWNAANGVTKYDYQICTNPEFDEPLHFITDKTSVIPSG
                     ITFGGHYYWRVRGRHTSDTSSWGVTRLFSIIPYPILKSPANGDTNVSTQPLFSWEPMT
                     GIESYYLQYDKDPNLTDGFISPPIGDSLSNYRCPYILEENTVYYWRMIATANSDTSQW
                     SPVFSFTTLKGIGMIENDLDKISIYPLPANNYLNIRFPSTMSGQATIQLIDLVGQKVI
                     ELTAEINSQNPYKLNVSNLKSGIYILRISNHGNSFIRKVSIE"
     gene            complement(3869..4042)
                     /locus_tag="PK976_00630"
     CDS             complement(3869..4042)
                     /locus_tag="PK976_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66425.1"
                     /translation="MISKEANKMKDKIKIMKEKIALILLFVFLIGMIASCKSEERCAA
                     YGEAKKYQVEKRQ"
BASE COUNT         1336 a          970 c          770 g         1294 t
ORIGIN      
        1 aacagcgatg aatcccaatg gcgaacggca aattatatag atggggaagg acaattgcag
       61 cccttgaatg gtttcgtttg ggacgtgaag gtgggaagca acggaattgt agtggcttcg
      121 atcgaaagta aggtttatgt ttcggaatta ggccaaccta atggctttat taatcaaagt
      181 acaaaacaag tgcttcccga taccgtttta aatccagaaa aactcccctc catagggctt
      241 ggacgtatag aattagctgt ggctccttcc aatcccgatt atatttatgc ttgcgctagc
      301 gatccttacg gctatttcga caatatttat cgttcgattg ataagggtat tacatgggaa
      361 attattttcc caggccattc ttctctgcta cccgatatct ttgtcgtgaa caacgtagct
      421 tatggtctgg tggccaatac tatcgttgtt taccccaaca atcccgaaga aatattagtt
      481 ggaggcataa atatgtggca tggcaaattt atcaatgaag gctattacta ttggggtaca
      541 gatgctatat caagttatgc tacgagtaaa ttctctccat tatatctcca tgagcttcat
      601 catacctatg tttttaaacc caatgatcca aaaacctttc ttataggcac agatggtggc
      661 atttctacta acatcgatgg agaactttat ttcaaaagtt tgaaccgcac ctacatgact
      721 tcacaattcg ttacactcaa tgtcaatggt ttcggaaatc ctattggagg tactctgggg
      781 aatgggatac aatatatagg cgatggcacc aattctccca taggcgcagt ggaagtgtgg
      841 aatggtgact ttaacaacag cggagaaggc ggttatgtag ccatttcaaa aatcaatccc
      901 aaggtattta tcttatctct taaaggtggg ccattccgcc gtacagaaga taaaggattc
      961 agtttctcta ctaccttcct caatagtggt atgtcagccc cggcaaatac ttactgcccc
     1021 atgatactct gggaatcgtt caatgaccct acaagcatgg attccactcg ttttaaggca
     1081 tctaaagaca ttaccgaagg agaagaaatc attgttcgta gccaaattta tgattatcca
     1141 ttcaaaacca ttgcccctca cgatatacac aagggcgacg tagtttattt taaagatcct
     1201 ctacaagcaa aaaccttcct cgctttgggc gataaagttt attataccaa agaaatcctc
     1261 gattttacaa aagaacctga atggtggcaa gtagctaagg tagtaggcac tgtttcgtgc
     1321 atgagccttt ccgccgatgc caatcacctt tatgtaggaa cagaaagtgg taatgttttc
     1381 cgcatatcta acctcacaca cgccaactcc gcagccactg cttcaattac ttcggccacc
     1441 tgtgtagtag ctactcaaca aatcaaatct ttccctaatc gtatagttac ttccattgca
     1501 gtcgacccca aaaatcctca acatatcctg gtaactcttg gtaactatgg attcgacgaa
     1561 tacgtatata atgttaccaa tgctctagat tcattaccca aattcacttc tgttcagact
     1621 aatcttccac acggaccagt ttattcgtct ttgatagaat tgaacaattc caatttaata
     1681 attctgggta cggaatatgg catctttctt accaacaata tttccgctcc aaactgggtg
     1741 aacgacaatg gccccatggg tagaattcct gtcaaggtct tattacagca aactgttaat
     1801 ggtggtggta ttttcgtgcc ctctgacgat ccaaatattc cgggagttta ttatcctgaa
     1861 atcaccaatt atggagtgat atatgctgct acatacggga gaggtgttta cagctctaat
     1921 ttcttcgtcg gaatcgacga acccaatata gataccccac ccactaatgc ccagcttgcc
     1981 atttatccga atcccgtccg aaacgaactc tcatttaccc taaaaggaga ttatgcttca
     2041 gaggttttca tcgaaatcta cgacttcagt ggaagaaaat caaaagaaaa tcatctgaaa
     2101 attcagccag gctccaatac gtttaccatg gatgtttcca atctgaaacg cggtacttat
     2161 atcctgcgtt gcatctccga cacctctatc tcgacgggta agttcatcat cgtaaaatag
     2221 aagtcttata atttttaaat agcatctaac aatgaaaaga atttttactt tcttctttct
     2281 attaatcggg ttttcggtaa tagctcaacc taagatttat acacccaccc tgcttttgcc
     2341 taataatggt gctacaaacc aaatgcccga cgttacattg agctggtcgg cagttacagg
     2401 tttgggcgta ataaagtaca aactcgaaat tgatgataac ccagatttca cctctcccaa
     2461 ggtattctat accgaatttc tgacgggtta taccatgagt aatcttcatt acggggtaca
     2521 ataccactgg agggtaaagg cttatgatct ggccactaat gattcttcat actggtcggc
     2581 tacacgaaac tttactgtct ttagtaaagt agataataag aaacccgaaa acaagacaaa
     2641 ggagattacg ccattagcca cgctggaatg gaaagaccgt ttggggccta atctggttac
     2701 gggaaataca tatttcaact atatcctcga taccgtagaa actttcgaca gtcctatggc
     2761 atatttaggg acagtgagcg gcagcgttta caaagccact acacccaaaa tgcgtttcgg
     2821 aactcgttat ttctggaagg taagagccat aaacgaaaaa gatacaagca attggtcgga
     2881 tgcatggtat ttcaggacac tggataccat cgttttaaaa gatcccgcca ataatgctac
     2941 aaatcaacag attaatctga ctttgtcatg gaatgcagcc aatggcgtta caaaatacga
     3001 ctatcaaata tgcaccaatc ccgaatttga tgaaccttta cattttataa ccgacaaaac
     3061 cagcgtaatt ccatcgggta tcacctttgg aggtcattac tattggagag tgagaggaag
     3121 acacacctcc gatacttcca gctggggcgt aacacgttta ttctctatca taccttatcc
     3181 catactgaaa tctccagcaa atggcgatac taacgtgagt acccaacctc tcttttcatg
     3241 ggaaccaatg acggggatag aatcttatta cctacagtat gataaagatc caaatcttac
     3301 cgacggattc atcagccctc ccataggtga ttccctatca aactacagat gcccttatat
     3361 actcgaagaa aataccgtct attattggag aatgatagct acagccaatt ctgatacttc
     3421 tcaatggtca cctgtattct cgtttactac cttaaaaggg attggaatga ttgaaaacga
     3481 tttggacaaa atcagcattt accctctccc agctaataat tacctgaaca tacgcttccc
     3541 ctctaccatg tcaggccaag ctacaattca acttatcgac ctggttggac aaaaagtgat
     3601 cgagcttaca gctgaaatta attcacaaaa cccttacaaa ctcaatgttt cgaatttgaa
     3661 aagtggaatt tatatccttc gcatttctaa tcatgggaac tcattcatcc gaaaagtctc
     3721 catagaataa cagaaatgtt ctgataccaa aaaagaaaag ggctgcatcg aaaacagccc
     3781 ttttcttttt ttttagacca ccctgtgttc gaaataaata actttcctct tattagcagt
     3841 gcttaaaata tagctaaatc accgaaatct attgacgctt ttctacctgg tatttcttgg
     3901 cttccccata agccgcacat ctttcttccg acttgcacga agctatcata cctattagga
     3961 atacaaacaa taaaatgaga gcaatcttct ctttcattat ctttatcttg tctttcatct
     4021 tgtttgcctc tttagagatc atgtttcaat tttctataac tgtataattt actaaaaaaa
     4081 ttcggtcttt aaagcacgga cattcattca aaatttcagg ctacaaaata aatgcttatt
     4141 atgataaaat tcaacttttt attgttaatc ttcggtaaat ttttcactcc aacgctgaga
     4201 agtcaacatt catcgaagtc tgtttctatt tctcgtcgat atgttttcat ttccttctat
     4261 ctttcaatcg gcctttatcg agatgatttc aggtaaaaaa ccactctttc accatcgaaa
     4321 ataattgcaa acgaaccgtt tattcagatg aagattctcc ccaccccgaa
//
LOCUS       DAORPD010000028         4366 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_3522_length_4366_cov_2.060079, whole genome shotgun sequence.
ACCESSION   DAORPD010000028 DAORPD010000000
VERSION     DAORPD010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4366
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3522_length_4366_cov_2.060079"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2377
                     /gene="pheT"
                     /locus_tag="PK976_00635"
     CDS             <1..2377
                     /gene="pheT"
                     /locus_tag="PK976_00635"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HRC66426.1"
                     /translation="LEVEAIETIEFIKGGLKGVVVGQVVSCQKHPDSDHLHLTKVDIG
                     NQEPLSVVCGAPNIAEGQKVLLAKVGTTLYFKNEPMAIKKAKIRGVVSEGMICAEDEL
                     GLGTSHEGILVLPPDAPIGMPAAQYFHLKEDVVFEIGLTPNRTDATSHIGVARDLHAS
                     IIARRPEIPSHFQKPSVENFKIDNHDFEIEVEIADAQACIRYSGLTLTNVRVGESPGW
                     LKELLQSVGIRPINNVVDITNFVLMETGQPLHAFDANKIKGRRVIVKTLPEGTPFTTL
                     DGIERRLDSTDLMICNAEEGMCIAGVFGGLESGISASTQSVFLESACFNPSYIRRTSR
                     RHQLFTDASFRYERGSDFNITIYALKRAAILLQEIAGANVSSEIKDAYPSEIEPVKVD
                     LRYSQVKRLVGQFIPKRRILSILQNLEYQILRESPQSLLISVPSCRVDVTREADVIED
                     ILRINGYNRIAFREELHTSLSYFSHPGPEQVKWEIAGLLAGNGFYEVINNSLSFSQHY
                     TLWPEDQRRTLIKLFNPLSRELNVMRGDMLPGLLENVAFNLNHQIDDLKIFEFGKIYR
                     FDGNLDPNADVSVRYTEPQQLAIAVTGLRENESWEKDHRRADFYYLHSILRKVLQRVG
                     VNLRLLKIVESQIPFYDQGIELFFGNELLAWVGIVAKAMLQKFDIRQEVVAAVVNWDK
                     VSSFPREMNIEFEEIPRFPEVRRDLALLLDNHIKFAEIEALAWKTENRLLRQINLFDI
                     YVGENIGPGKKSYAISFILQDKEKTLTDEDIDKTMAKLITAFEQKLGAQLR"
     gene            2577..4223
                     /locus_tag="PK976_00640"
     CDS             2577..4223
                     /locus_tag="PK976_00640"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HRC66427.1"
                     /translation="MNNNKTLVYSVSPSGEEFPLPQNDDYIAERKRIESLVGEARSER
                     KEIVVVMGLGFVGAVMAAIVADTRNSMGEYSKFVIGCQRPSKRSYWKIPILNTGVSPI
                     KADDPEVDELIHRTVVEKQTLTATFNDDCLEFADVVVVDVQCDFTKFELGNMRSGTAD
                     MAALEATMKTIGKKIPPQCLVLIETTVAPGTTEFVAWPILKKAFARRGIESQPLLSHS
                     FERVMPGKEYVASIRDFWRVASGCNLEARQRVEKFLREVVNTEKFPLTIMDRPIESET
                     TKIVENSYRATILAYLNEWSLFAERNGVDLIKVIDAIKMRPTHSNIIFPGPGIGGYCL
                     PKDGGLGYWAYKHILGFDDADKLFRITPEAIDINDTRSLHVAELTRDALRNMGRYIAG
                     SDVLVCGASYRQDVGDTRYSGSEIIVRKLTEMGADIRVHDPFVDVWYEFVNQDENAHS
                     WKRFFHYQDGLKNLQILKDLPKAMEGVEAVVLAVPHAPYKSLSPDDAVKWAGGKIAVI
                     DCFGILPDDKIRRYFQLGCEVKALGRGHIQRIKREVRGLE"
BASE COUNT         1262 a          884 c          961 g         1259 t
ORIGIN      
        1 cctcgaagtc gaagccatcg aaaccataga atttataaaa ggagggctca agggcgttgt
       61 agtaggtcag gtagtctctt gccagaaaca tcccgatagc gaccacctcc atcttacaaa
      121 agtggatata ggaaaccaag aacctttgtc ggttgtttgt ggagctccta atattgcaga
      181 aggacagaag gtgttactcg ctaaagttgg aactactctt tattttaaaa atgaaccaat
      241 ggcgattaaa aaggcaaaga taaggggagt ggtttctgag ggaatgatct gtgccgaaga
      301 tgaattgggt cttggtactt cacacgaagg cattctggtt ttgcctcccg atgctccgat
      361 cggtatgcca gccgcccaat attttcatct caaagaagat gtggttttcg aaataggttt
      421 gactcctaac cgcaccgatg ctacttcaca tataggtgtt gcacgtgacc ttcacgctag
      481 cataatcgcc cgcaggcccg aaattccatc tcattttcaa aaaccttcgg tcgagaattt
      541 caaaatcgat aatcatgact tcgagatcga agtggaaatt gcagatgctc aagcttgcat
      601 tcgttattcg ggactcaccc tgacaaatgt tcgagtagga gaatcgcctg gctggctcaa
      661 agaactgttg cagtcggtag gaatcagacc cattaataat gtagtcgaca ttaccaactt
      721 cgttttgatg gaaactggcc aacccctgca tgcctttgat gccaacaaaa tcaagggaag
      781 aagagtgatt gtgaaaacct tacctgaagg aactcctttt acaactctcg atggcatcga
      841 acgaagactc gattctaccg acctgatgat atgcaatgcc gaagaaggaa tgtgcattgc
      901 tggtgtattc gggggccttg aatctggtat ctctgcttcc acacaaagtg tttttctcga
      961 aagtgcatgt ttcaatcctt cctatatcag aagaacttct agacggcatc aattatttac
     1021 ggatgcttca ttccgttacg aaagaggaag cgattttaac attaccatct atgcattgaa
     1081 acgcgcggca atacttcttc aggaaattgc aggggctaat gtttcgtctg aaatcaagga
     1141 tgcctatcct tctgaaattg aacctgtcaa agtcgatttg cgatattctc aagtgaaacg
     1201 tcttgtgggt caattcattc ccaaacgacg gatactatcc atactccaaa atttagaata
     1261 tcaaatttta agagaatcgc cgcaatcatt actgataagc gtgccaagtt gccgtgtcga
     1321 tgtaacccgc gaagccgacg taatcgaaga tattcttcgt attaatggtt ataatcggat
     1381 tgcttttcga gaagaattgc atacttcact ctcctatttc tctcatcctg gtcctgagca
     1441 ggttaaatgg gaaattgccg gccttttggc cgggaatggt ttttatgaag taataaataa
     1501 tagcttgagc ttttcacagc attatacact ttggcctgaa gatcaacgga gaactctcat
     1561 aaaattgttc aatcctctta gccgcgaatt gaatgttatg cggggggata tgctacctgg
     1621 acttctcgaa aatgttgcct tcaatttgaa ccatcaaatc gacgatttga aaatttttga
     1681 atttgggaag atatatcgtt tcgatggaaa ccttgaccct aatgctgatg taagcgttcg
     1741 atatactgaa ccccagcagc ttgcaatagc agtaactggt ttgcgggaaa atgagagctg
     1801 ggagaaagac catcgtcggg ccgattttta ttacctgcat tctattctta gaaaggtatt
     1861 gcaacgtgta ggtgtcaatt tgcgcttgct gaaaatcgtt gaatcccaaa ttccttttta
     1921 tgatcaaggt atagaactgt ttttcggaaa tgagcttttg gcatgggtag gaattgttgc
     1981 caaggcaatg ctccaaaagt tcgatatacg tcaggaagtt gttgcagccg ttgtaaactg
     2041 ggataaagtt tcatcattcc ccagagaaat gaatatcgaa ttcgaggaaa tacctcgttt
     2101 tcctgaggta cgtcgtgatc tggctctttt actcgacaat catataaaat ttgctgaaat
     2161 agaagctctg gcttggaaaa ccgaaaatcg cctccttcga caaatcaatc ttttcgatat
     2221 ctatgtaggt gaaaacatcg gaccaggtaa aaaatcctat gcaataagtt ttatccttca
     2281 agataaggag aaaacgctaa cggatgagga tatcgataaa accatggcaa aacttatcac
     2341 ggcattcgag cagaagttgg gtgcacaact gcgctaatac cccaaaactc gaatcctcga
     2401 tttggtttta taccctaaga cccaatgacg aacttattca ttattatcca attaattgga
     2461 aggatagtat actgttcatc taaaagattt ttaacataaa ctttgtcctt ttgcgatttt
     2521 tatctacttt ggtggcctta ttctaaaatc tattcttatc taaatttcaa taaaatatga
     2581 ataacaacaa aaccttggtt tattctgtca gcccttcagg tgaagagttt ccactacctc
     2641 aaaatgatga ttatattgct gaaagaaagc gtattgagtc attagttggg gaagctcgta
     2701 gtgaaagaaa ggaaatcgtt gtggtaatgg gattaggttt tgtaggagcc gttatggcgg
     2761 caatagttgc cgatacccgc aatagcatgg gagagtattc gaaatttgta ataggatgcc
     2821 agcgtccaag caaaagaagc tattggaaaa ttccgatttt aaacactggt gtttcaccta
     2881 ttaaagccga tgatccggag gtggatgaat tgattcatcg gaccgttgtt gaaaagcaaa
     2941 cattgacggc tactttcaat gatgattgtc tcgaatttgc cgatgttgtc gtcgttgatg
     3001 tacagtgtga ttttactaaa ttcgaactcg gaaatatgcg ttcaggcact gccgatatgg
     3061 ctgccctcga agccaccatg aaaacgattg gtaaaaagat accgcctcag tgcctcgtac
     3121 ttatcgaaac taccgtagcg cctgggacta ccgaatttgt tgcctggcct atactgaaaa
     3181 aagcatttgc cagacgtgga atcgaatccc aaccattgct atctcatagt tttgaacgtg
     3241 tcatgcccgg gaaagaatac gtcgccagta ttcgggattt ctggagagta gcctctggct
     3301 gcaaccttga agctcgccaa agagtcgaaa aatttctcag agaagtcgtc aataccgaga
     3361 aatttccttt aaccatcatg gatagaccta tcgaatcgga aaccaccaaa attgtcgaaa
     3421 attcctatcg ggctaccatt cttgcctatc ttaatgaatg gagtctgttt gctgagcgta
     3481 atggagtcga cctgatcaaa gtgatcgatg ccattaaaat gaggcctaca cacagcaata
     3541 ttattttccc cggccctggc attggtggtt actgtctgcc aaaagacgga ggattaggct
     3601 attgggctta caaacatatt ttgggattcg atgacgccga taaacttttt cgcattaccc
     3661 ctgaagctat cgacatcaat gatacaagaa gtttacatgt agccgaactc acccgcgacg
     3721 ccttgcgtaa tatgggacgt tatatagccg gctctgacgt gcttgtttgt ggggcctcct
     3781 acaggcagga tgtgggcgat acccgttaca gcggaagtga aattatcgtt aggaaactta
     3841 ctgaaatggg cgccgatata cgcgtacacg accccttcgt ggatgtttgg tatgaatttg
     3901 tcaatcagga tgaaaatgca cattcatgga aacgtttctt ccattatcag gatggattga
     3961 aaaatttgca gattctcaag gatcttccta aagccatgga aggggttgaa gcagtcgtat
     4021 tggctgtacc tcacgcacct tataaaagcc tttcacctga cgatgcagta aaatgggcgg
     4081 gaggtaaaat tgcggttatc gattgttttg gtattcttcc tgatgacaag attcgccgct
     4141 atttccagct cggttgcgag gtaaaagcct taggaagagg ccatattcag agaattaagc
     4201 gtgaagtcag gggactggaa taatttggtt tcgtacatcc gaggaagtct ccatttcatc
     4261 agttagaatt tttatactga gagagcttta ttttgacaag gtttttaccg agtgtaattc
     4321 cttttcctgt tttccgcact tttttgcaac acccttataa gtattt
//
LOCUS       DAORPD010000029         4315 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_3570_length_4315_cov_2.600000, whole genome shotgun sequence.
ACCESSION   DAORPD010000029 DAORPD010000000
VERSION     DAORPD010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4315
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3570_length_4315_cov_2.600000"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1302)
                     /locus_tag="PK976_00645"
     CDS             complement(<1..1302)
                     /locus_tag="PK976_00645"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HRC66428.1"
                     /translation="MENLSITHQLFRMSKLPFIQGYLLKKVDKYLYDIIVEENKRNLE
                     SVKMRKYHFISAMMHSAMKNVRKGRISTYAIQRLNEVLVENAFFKAPEQMKNAKEAFK
                     DKFGYDAPSFITLSPTQACNLKCSGCYASSDPHAMASLPYSIVDRLTGEVHDSFGSRF
                     ITISGGEPFLYKSEGHTLIDLFDKYKDMFFLVYTNGTLITKELAHELARVGNATPAIS
                     VEGFEKETDDRRGKHVHKRILETFHNLRKSGVPFGISVTASNNNIQTLLQDKFYDYYF
                     DGLGATYMWLFHFFPIGRGKEQFDLMLKPADRLKLYEMWEKQIAQKKHCIADFWNSGV
                     LTCGCIAYGGNRGFLYIDWNGNIMPCVFVPYYQHNIIDLYNSGKDLTYALQSDFMKNG
                     RKWQQEYGLNNQKSPNNWLMPCSIRDHYDNFRKNILTPEAKG"
     gene            1836..3350
                     /locus_tag="PK976_00650"
     CDS             1836..3350
                     /locus_tag="PK976_00650"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HRC66429.1"
                     /translation="MRELLIDAADKYGKNTAYIYRDKEYDFITLKDCSLQLANSLLDM
                     GFRKNDKIAIYLPNCIEYIYSYFAIYSLGCVAVPIDFALSLTEVVSISNHCGLKGIIT
                     TKQAKFNLFELKGRISTLKEIITIEDDSRYVSFGNLTKSGKDGLNDQNIDSQMFSSIF
                     YTSGTMGRPKGALWNYEHIHLGAEQLKYFTTYEYLKTILRIDETERTIAAVPLSHSAG
                     ILFVMTAVKYGMSHVIMPRFSPLDFIKMVDKWKVTGVFMVPPMYYAILYLKEVESYSL
                     DSLRWANVFGAPSSYDLMMRFQKLCPNAVILNGWGMTEIIPPISASSPENIKSVGRHV
                     PNVELKIFDSKGNAISTGEVGELVARGKSVFCGYYNEPELNQEAFKDGWFYTGDLGRF
                     DEKGNLYIVGKSKDTIKVGGELVWAAEIEEILLKHPCIKEAAAVGVPDELRGEVVKCY
                     IVLKEGIVLPQKYVMEFLREHLSRFKMPRQIEYLDELPKTGSGKINKAALIKRN"
     gene            3352..4251
                     /locus_tag="PK976_00655"
     CDS             3352..4251
                     /locus_tag="PK976_00655"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRC66430.1"
                     /translation="MSIHSNTLQKSLGRFFIELSLALTRILPKTTFRALLHTLSAVLI
                     PKKFKERAKTSLRILYGDTVEDKTLDKKAKELIEFFLNQAVEMAYYCKMGGMPAYEDF
                     IEDISGKEYLEEALKQQKGVIALTAHIGNFTMLTHTLTLAGFPNCATIMREQSDVKTE
                     KLFTELRTEIGIKWINENESKSNVLALVKFLKKNGILIMLSDQRLIGGVKVRFLRREK
                     LTAKGPAALALRVGCPVLPIYSIVTSNGKNHLVIKPPIPLIKTGDTGKDIFENTQIFM
                     DEIGNIIKEHPDQWYAFSRWWRV"
BASE COUNT         1359 a          759 c          908 g         1289 t
ORIGIN      
        1 accttttgcc tccggggtaa gaatattttt tcgaaagtta tcatagtggt cgcgtataga
       61 gcaaggcatg agccagttgt taggcgattt ttgattgttt aatccatatt cctgttgcca
      121 ttttcggcca tttttcatga aatcggattg aagggcataa gtgagatcct tgcctgagtt
      181 gtataaatcg atgatattgt gttgatagta agggacgaac acacagggca tgatgtttcc
      241 attccagtcg atatagagga agccgcgatt tccgccatag gcaatacatc cgcaggtaag
      301 cactccgcta ttccagaaat cggctatgca atgttttttc tgggcaattt gtttttccca
      361 catctcgtaa agtttgaggc gatcggcggg ttttagcatg aggtcgaatt gttctttgcc
      421 tctgccaatc ggaaagaaat gaaacagcca catataggtt gctcccaatc catcgaaata
      481 ataatcataa aatttgtcct gaagtaaggt ttggatatta ttattggaag cagtgactga
      541 aattccgaag ggtactccag atttgcggag gttatggaat gtctcaagga ttcttttatg
      601 gacgtgtttg ccacgtctgt catcggtttc tttttcgaat ccttccaccg aaatggcggg
      661 agtggcattg cctactcgtg caagttcatg tgctaattct ttggtaatca gtgtgccatt
      721 cgtataaaca aggaagaaca tatccttata tttatcgaat aaatcgatta aagtatgtcc
      781 ttcgctttta taaaggaaag gttcacctcc actgatggta ataaaacggc ttccaaagga
      841 atcatgaact tcgccggtta aacggtcgac gatagaataa gggagactag ccatagcatg
      901 aggatcggag ctggcataac atccgctgca ttttaaatta caggcctgag taggtgaaag
      961 cgttataaaa ctgggggcat cgtagccaaa tttatctttg aatgcctctt tggcattttt
     1021 catttgctcg ggcgctttga aaaaagcatt ctcaactagc acctcattga gcctttgaat
     1081 ggcataggtg gatattcgcc ctttcctcac attcttcatc gccgagtgca tcatggccga
     1141 aatgaaatga tacttcctca tttttaccga ttcgagattt cgcttatttt cttcgacgat
     1201 aatatcataa agatacttat cgactttttt gagcaagtat ccctgaataa aaggtaattt
     1261 cgacattctg aacagctgat gggtaatcga caaattttcc atgatatatg tttttaaaac
     1321 gttctaattc aattattccg taaacaaaac ccggtcaaaa ttcttcattc cattttgcct
     1381 cttttgaaaa ccatgacaaa tttaagcaac aatgaagaaa atgagataca aaatcgtaat
     1441 caaattcaag ataattctgt tataaattgt tgaagaaatt atttagagaa taaaaatgaa
     1501 accgtatata tgttttaaag gattatataa aataaaaatc gcgaagtagc atcaattgta
     1561 ttttcttcga tagtagtagg cacctttcgt aaatttaact aaaggaaaga taaagaaagg
     1621 gttatgagta ttaaagtaaa atcgataaat tagggttaat caatgttttg atttgcattc
     1681 cgcatgggga tataaatctt attttatatg tatattaata taagataata ccttaactgt
     1741 agtatttggt tattttaatt taatcttacc tttaattttt ttcatgtact tttgctaagc
     1801 acgtataaaa gcgcttaatg gagaaaaata tggacatgag agaacttttg attgatgcag
     1861 cagacaagta tggcaaaaac acagcctata tttatcgaga taaagaatat gatttcatca
     1921 cccttaaaga ttgttcttta caactggcca atagtttact cgatatgggt ttcaggaaaa
     1981 atgacaaaat tgccatatac ctgcctaatt gtatagaata catttattct tattttgcca
     2041 tttattcact gggttgtgtt gcggtaccca ttgattttgc tttaagtctc acagaagtgg
     2101 tttccatatc gaatcattgc ggtttgaaag ggattatcac tactaagcag gcaaaattca
     2161 atcttttcga attgaagggg cgcattagta cgcttaaaga aatcattacg atagaagatg
     2221 attcgcgata cgtatcattt ggtaatctga caaaaagcgg gaaagatgga ttgaacgatc
     2281 agaacattga ttcccaaatg ttttcgagta ttttctacac ttcgggtact atgggcaggc
     2341 ccaagggcgc cttatggaat tacgagcata ttcatttagg tgccgaacaa ctgaaatatt
     2401 ttaccaccta cgaatatctg aaaaccattt taaggataga tgaaaccgag cgtacaatag
     2461 ctgcagttcc tctatctcac tcagcaggga tcttattcgt gatgactgcg gtaaaatatg
     2521 gaatgagcca tgtgattatg cctcgttttt cacctttgga ttttataaag atggtagaca
     2581 aatggaaagt gacgggcgta tttatggttc cccccatgta ttatgccatt ttgtatttaa
     2641 aggaagtgga atcgtattct ttggatagtt tacgttgggc gaatgtattt ggggcaccat
     2701 catcttacga tttgatgatg cgttttcaaa aactttgccc caatgccgta atattaaatg
     2761 gttggggaat gactgagatt attccgccca tatcggcttc ttcgcctgaa aacatcaagt
     2821 cggtaggcag gcatgtaccc aatgtggaac tgaaaatttt cgattcgaaa ggaaatgcaa
     2881 tatctaccgg agaagtcggc gagctggttg ccagaggcaa atccgtattt tgtggatact
     2941 acaatgaacc cgagttaaat caggaggctt tcaaggatgg atggttctac actggcgact
     3001 taggccggtt cgacgagaag gggaatttgt atatcgttgg aaaatcaaaa gatacaatta
     3061 aagtgggagg cgagttggtt tgggcggccg aaatcgaaga gattctgctg aaacatcctt
     3121 gcatcaaaga agctgctgct gttggtgttc ctgatgaact gagaggcgaa gtggtgaaat
     3181 gttatatagt attgaaagaa ggtattgtgt tgcctcaaaa atatgtgatg gaattcctca
     3241 gggaacacct ttcgagattc aaaatgccaa ggcaaataga atatctcgat gagcttccca
     3301 aaactggctc gggaaaaatc aacaaggctg ctctgatcaa acggaactga gatgtctata
     3361 catagcaata ctttgcagaa atcgctggga aggtttttta tcgagctatc attggcactt
     3421 acaagaatcc tgcccaaaac aaccttcaga gcactcctgc atacattatc tgcggttttg
     3481 attcccaaaa aattcaaaga aagagccaaa acaagcctga gaattcttta tggggataca
     3541 gtagaagata aaactttaga taagaaggcg aaagaattga ttgagttttt cttgaatcaa
     3601 gcagtcgaaa tggcttacta ctgcaagatg ggtggtatgc ctgcttatga agattttatc
     3661 gaagatatca gtggaaagga atatttagaa gaagccttga aacagcagaa aggcgttatt
     3721 gctcttacgg ctcatattgg aaattttacc atgctcactc atactctcac cctggcagga
     3781 ttccctaatt gtgccactat catgcgagaa caaagtgacg taaagacaga aaaacttttt
     3841 acagaactca gaactgagat tggtataaaa tggataaatg agaacgaatc gaagagtaat
     3901 gtactcgctc ttgtcaaatt cttgaaaaaa aatggcattt taattatgtt aagcgaccaa
     3961 agattgatag gaggagtaaa agtaagattc ttaagacgtg aaaaacttac ggccaaagga
     4021 cctgcagcct tggctctgag agtgggctgc ccagtattac ccatatacag tatagtgaca
     4081 agcaacggca aaaaccatct cgtcataaaa cctcctatcc cattgataaa aactggtgac
     4141 acaggaaaag acatcttcga aaatacacag atttttatgg acgagattgg aaacatcata
     4201 aaagaacatc ccgatcaatg gtatgcattc agccgatggt ggagagtttg agaaacttat
     4261 cccaacttga gcgccttagg gttgtaactt ccttgtatac agcaagttga ccttt
//
LOCUS       DAORPD010000030         4296 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_3594_length_4296_cov_2.339543, whole genome shotgun sequence.
ACCESSION   DAORPD010000030 DAORPD010000000
VERSION     DAORPD010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4296
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3594_length_4296_cov_2.339543"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="PK976_00660"
     CDS             <1..585
                     /locus_tag="PK976_00660"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HRC66431.1"
                     /translation="VALKIFYSTPMFSNSSILYFLEQIRDDSHIESHKRMAQNLLERF
                     TRLKPGSLLPDKVVKSYKGDSIRLREYSQKPLYIVFARKECLTCLANLEMLRPLSVKF
                     KDQVNFLAIFTSHDTLADARTIEQMNYPWPSVLGGRNYELLRAFEAYALPLEMLISPG
                     GIIAAYPAYRVNEGLEITLQKMLMRALPSGSLHH"
     gene            complement(530..1060)
                     /locus_tag="PK976_00665"
     CDS             complement(530..1060)
                     /locus_tag="PK976_00665"
                     /inference="COORDINATES: protein motif:HMM:NF015559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HRC66432.1"
                     /translation="MNLDVRPTTDMAKVGLFNVIENAWEMEGLHVLDLFAGTGSISFE
                     FISRGSVEVVAVDNNPRCIDWMNEASKGMGFDNLLLVHTDVFSYLQKPVQQFDIIFAD
                     PPYDFSKTTFIPDLIFRNHFLYPGGWLIIEHPKKIDFSHHPFFIKKKNYGRVNFSFFS
                     DEGSQTVERASTSSAK"
     gene            complement(1096..1986)
                     /locus_tag="PK976_00670"
     CDS             complement(1096..1986)
                     /locus_tag="PK976_00670"
                     /inference="COORDINATES: protein motif:HMM:NF024271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3822 family protein"
                     /protein_id="HRC66433.1"
                     /translation="MNETVKTLYQVSDHSFNSNQTQDYHLSILLSGDGFSYVAGNKEP
                     RRLIELGSFEFVIPLDVNRALRNEKALCLFSNFVIEHAWLGNPFGHSGIWIENRYSTL
                     IPEALYDEHALHEYLSFNLQIPQDYILRSEYIPSIEAWNVFALPAEWQKQIAFILPSV
                     KFHHASSCFIEAIIAHGNIGELNENVFIYPHRFYFEMAYTVKNKLIFYNTFPYSTQED
                     FIYFVLFGFEQLGLNPEIVPVILMGEIDTGSLLHLILIRYVRNVNFASRNKDVIYTYA
                     FDQIPSHYFLNIIYSLQCEL"
     gene            complement(2020..2616)
                     /locus_tag="PK976_00675"
     CDS             complement(2020..2616)
                     /locus_tag="PK976_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66434.1"
                     /translation="MKNAIRISLGIVIIVLCYLIYDSIMTPLRFEKEKNYRYGVIIQK
                     LKDIREIEKMYKNANKKYCGSFDTLFVFAKTAKIPVVRMIPDPTDTTFTRSISDTIGY
                     TLVYDSLFGKREGFRIEDIAINPFNPEVKIKLEAGFIDKGGLKVPVFMASALNVDILK
                     GLDEQLIRNYNASLEALNKFPGLKVGSMTEVSIDGNWE"
     gene            complement(2969..>4296)
                     /gene="rho"
                     /locus_tag="PK976_00680"
     CDS             complement(2969..>4296)
                     /gene="rho"
                     /locus_tag="PK976_00680"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008999884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HRC66435.1"
                     /translation="NGNDNDKEENIASEERLEPEAAVESSLTDKLDYKEIIARERAQR
                     EINLRYQRTPFEKYERKRDDFYPFSMEGIVSSEGVLELMPEGHGFLRSSDYNYLPSPD
                     DIYMASNQIKMLGLKTGDTIKGMIRPPRDNEKFFPLVKVELINGLQPEKIRDRVPFDF
                     LTPLFPYKKFKLTGHPDATLTSRIIDMFAPIGKGQRGLIVAQPKTGKTMILKEIANAI
                     AFNHPDVYLIVLLIDERPEEVTDMQRSVKAEVISSTFDEPAERHVRISGIVLEKAKRL
                     VECGHDVVILLDSITRLARAYNTVSPASGKVLSGGVEANALQKPKRFFGAARQIENGG
                     SLTIVSTALIDTGSKMDEVIFEEFKGTGNMELQLDRKLSNKRIFPAIDIVASSTRRDD
                     LLLPKEVLQRVWILRNHLADMTSQEAMEFLQDKMKFTQSNEEFLASMNG"
BASE COUNT         1250 a          832 c          892 g         1322 t
ORIGIN      
        1 gtggctttga aaatatttta ttccacccct atgttttcca actccagtat cctttatttt
       61 ctggagcaga tacgggatga ttcgcacata gagagtcata agcgaatggc tcagaatcta
      121 ctcgaaagat ttacaagatt gaaacccggg agtttgctac ctgataaagt tgtgaaaagt
      181 tataagggag acagtattcg attgcgcgaa tatagccaga aaccattgta tattgtattt
      241 gcacgtaagg aatgcctgac atgccttgcg aatctcgaaa tgctaaggcc cctttcagta
      301 aaattcaagg atcaagtaaa ttttttggcc atttttacaa gtcatgatac acttgcagat
      361 gctcgaacaa tagagcagat gaattatccc tggccgagcg tattaggagg gcggaactac
      421 gaattgcttc gagcttttga ggcctatgcc ttaccacttg aaatgctaat ttctcccgga
      481 ggtatcatag ctgcttatcc agcttaccgg gtaaatgaag gtcttgaaat tactttgcag
      541 aagatgttga tgcgcgctct accgtctggg agccttcatc actgaaaaaa ctaaaattta
      601 cccttccata attcttcttt tttatgaaga atggatgatg agaaaagtct attttttttg
      661 gatgttcgat gatgagccat ccaccgggat acaagaaatg attccgaaaa atcaagtctg
      721 gaataaaggt agttttcgaa aagtcataag ggggatcggc aaaaatgata tcgaattgct
      781 gcacaggctt ttgtaaatag gaaaacacgt cggtatgcac cagcaataaa ttatcgaaac
      841 ccattccttt tgaggcttca ttcatccaat cgatgcatct aggattatta tcgaccgcaa
      901 ccacttcgac acttcccctc gaaatgaact caaacgaaat gcttcccgta cctgcaaata
      961 aatcgagcac gtgaaggcct tccatctccc atgcattttc gatgacattg aaaagcccca
     1021 ctttggccat atcggtagtt ggccttacat cgagattcat tggaggatga agtattttgg
     1081 cctttcgttt ccctcttata attcgcattg caaagaatag ataatgttca gaaaataatg
     1141 agaaggtatt tgatcaaaag cataggtgta aataacatct ttgtttcggc tggcaaaatt
     1201 aacatttcga acgtaacgga tgaggatgag atgaagtagt gaaccggtat cgatttcacc
     1261 catcagtatt accggcacta tctcaggatt caggccaagc tgttcgaaac cgaatagaac
     1321 gaaatagata aaatcttctt gggtagaata agggaaggta ttataaaaga tcagtttgtt
     1381 tttaacagta taagccatct cgaaataaaa gcgatgggga tagatgaaca cgttttcatt
     1441 caactcaccg atattgccgt gggctattat tgcttcaata aaacacgatg aagcatgatg
     1501 aaacttaact gaaggcaaga taaaagcgat ttgtttttgc cattcggctg ggagggcaaa
     1561 tacattccat gcttctatgg agggtatata ttcacttctt aatatataat cctgaggaat
     1621 ttgcaggttg aagcttaaat attcgtggag ggcatgttca tcgtaaagtg cttcaggaat
     1681 tagcgtggaa taacgatttt cgatccatat tcctgaatgt ccaaatggat ttcctaacca
     1741 agcatgctcg atcacaaaat tcgaaaatag acataaggcc ttttcatttc tcaaggctct
     1801 gtttacatcg aggggtatta caaactcaaa actgcctaat tcaatcagcc ggcgaggttc
     1861 tttattcccg gctacatacg aaaatccatc ccccgaaaga aggatggata aatgatagtc
     1921 ttgcgtctga ttggaattga aactgtggtc gctaacttga taaagggttt ttactgtttc
     1981 attcacatca gcaagttttt gtaaccggcc tgggtaggtc tactcccagt ttccatcaat
     2041 ggatacttcg gtcatcgaac ctactttcag acctgggaat ttgttcaaag cttctaagga
     2101 tgcattataa ttacgaatga gctgttcatc taatcctttg agaatatcta cattcagcgc
     2161 cgaagccata aacacaggta ctttaagccc tcccttgtcg ataaaaccgg cttcaagttt
     2221 tatctttacc tcaggattga aaggattgat agcgatatcc tctattctga atccttcccg
     2281 tttcccgaat aaagagtcgt aaaccagtgt ataaccaatg gtatcgctaa tggagcgagt
     2341 aaaggtagta tcggtaggat cggggatcat acgaactacg ggaatcttgg cagttttagc
     2401 aaaaacaaag agggtgtcga aactgccaca atatttttta ttagcattct tatacatttt
     2461 ttcgatctca cgtatatctt ttagtttttg aatgatcact ccatatcggt aattcttttc
     2521 cttttcgaaa cggagaggag tcattatact gtcatagatc aagtagcata gaactatgat
     2581 gactattccg agggagattc tgatggcatt cttcatagga tttaaatttt tatgcgatgc
     2641 aaaaatagaa gttttttata aaaaaaaata ctcgatatat aacgataaaa gaattatttt
     2701 attgagagaa gacaattcga tttttagcaa ttcaaaactt attgattatc aacataaaat
     2761 ttcgaacact tattttaatg ataccaaaga agccaattgc aatcgaattt ttgcttgatt
     2821 aaattgagtg tctatctatc gacttcatcg tgattgatgt tttgtaggaa gtcatctcga
     2881 aaatttcgat gaagcaattc tatttgtaga agtgaatgtt cttcgataaa tttcgtggaa
     2941 tacgatttag gtaataagtc gaaagtcatc aaccattcat cgaagccagg aattcttcgt
     3001 tggactgggt aaatttcatt ttatcctgca aaaattccat ggcttcctga gaagtcatat
     3061 ctgcaagatg attccgtaga atccacactc tttgcagaac ttctttgggc agcagtaaat
     3121 cgtcgcggcg ggttgaggaa gcgacaatgt cgatagcagg gaagatgcgt ttattggaga
     3181 gtttacggtc gagttgcaat tccatgtttc cggtgccttt gaattcttcg aagatcactt
     3241 cgtccatttt cgaacctgta tcgatgagtg cagtgctcac aatagtgagt gagccaccat
     3301 tttcgatttg tcgtgcagca ccgaagaagc gtttgggttt ttgcagggca ttggcttcga
     3361 ctcctcctga aagtactttg cccgaagcag gcgatacggt attgtaagct cttgctaaac
     3421 gagtaatcga atcgagcaga atgaccacat catgtccaca ttctaccaaa cgtttggctt
     3481 tttcgaggac gatacccgaa attctgacat ggcgttcagc aggttcatcg aaagtagagc
     3541 taattacttc tgcttttacg cttcgttgca tatcggtaac ttcttcgggg cgttcgtcga
     3601 ttaaaagtac aatcagataa acatcgggat gattgaaggc aatggcattg gcaatttctt
     3661 tcagaatcat tgtcttgcct gtttttggct gggctacgat caatcctctt tgtcctttgc
     3721 cgatgggggc aaacatgtcg ataattctcg aagtaagtgt agcatcggga tgcccagtga
     3781 gcttgaattt tttataaggg aacaaaggtg tcagaaaatc gaaagggaca cggtcgcgga
     3841 tcttttccgg ttgcaaccca ttgatcaact ctactttcac cagaggaaaa aacttctcgt
     3901 tatctcgggg aggcctgatc attcctttta tggtatctcc cgttttaaga cccagcattt
     3961 tgatttgatt ggaagccata tagatatcat cgggtgaagg aagataattg taatcggaag
     4021 aacgcagaaa tccgtgtcct tcgggcatta gctcgagtac accttcgctg ctgactattc
     4081 cttccatgct gaatgggtag aaatcatcgc gtttacgttc gtatttttcg aaaggtgtcc
     4141 gctggtaacg caggtttatt tctctttgag ccctttctct ggctataatt tctttataat
     4201 ccagtttatc cgttaaagaa gattcaaccg cagcttctgg ttcgagtctt tcttcgcttg
     4261 caatgttttc ttctttatcg ttatcatttc cgtttt
//
LOCUS       DAORPD010000031         4250 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_3639_length_4250_cov_2.461979, whole genome shotgun sequence.
ACCESSION   DAORPD010000031 DAORPD010000000
VERSION     DAORPD010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4250
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3639_length_4250_cov_2.461979"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="PK976_00685"
     CDS             complement(<1..397)
                     /locus_tag="PK976_00685"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HRC66436.1"
                     /translation="METHFTGKPDIVMGKSLLCRIKTQFTVYACLLRWINLLMILLIV
                     LIIRYGFFLPLYMAIGLASPLSHTIYFLVVLSIVLITAAGYIVNDIYDQKIDRLNKPT
                     RLVIGQAVSVSRGWILYVALNIFGLISGGI"
     gene            complement(375..854)
                     /locus_tag="PK976_00690"
     CDS             complement(375..854)
                     /locus_tag="PK976_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005936823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonate 8-phosphate
                     phosphatase"
                     /protein_id="HRC66437.1"
                     /translation="MFDYDGVLTTGEIFILPDGEVLRGGYVRDGYAIQHAIKNGYRIV
                     IISGGKSDSMVHRLNALKVNDFFLGIADKLSIFNEYIHLHHIEPQGVLYMGDDIPDLT
                     VMQQVGLPCCPADASEEIKAISLYISHREGGKGCVRDVIEQVMKLQGKWMNGDAFYW"
     gene            complement(890..1681)
                     /locus_tag="PK976_00695"
     CDS             complement(890..1681)
                     /locus_tag="PK976_00695"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HRC66438.1"
                     /translation="MEKTENEVIIIGAGNVGTHLAHQLDKKNFTIRGICSRTIQSARQ
                     LAKYYVCESWTNAALIPPDAQYYFICVNDEAIAKVADAMPQTNGLVMHTSGSTPIEVL
                     SRFPNHGVFYPIQTFTKNHETDFSLIPICIEASNKKNLNILKKLALQFSNNIIEANSA
                     QRKHIHLSAVFACNFTNYMYVIAHDILNEQKLPFSILLPLILESFEKVTLMEPWQAQT
                     GPAVRGDSSIIEEHIRMLSENPDYEVIYRIISEQITKRKNKEKTE"
     gene            complement(1734..3038)
                     /locus_tag="PK976_00700"
     CDS             complement(1734..3038)
                     /locus_tag="PK976_00700"
                     /inference="COORDINATES: protein motif:HMM:NF028426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5103 domain-containing protein"
                     /protein_id="HRC66439.1"
                     /translation="MKNAFASILLIIIVFSSILAQDDIAFYSQKALRTQNRIYNPDIK
                     TVLIFPTGYPLEMPVISLNSDKTLQLQFDDLAGGVKNFQYTFLHCDANWEPSQLRMNE
                     YMEGFDSDEIRDYKFSFNTTTSYTHYSLIFPNDRIRLTKSGNYLLVVYLDSPTQPEFS
                     LRFIIYEPRVIIQDVKIGRAHLPAYMNTKHEVDFTIRPVKYKIPVPDRDLTIVILQNW
                     RWDNALTIKQPRNITPDLLDYDYEEENLFDAGNQYRSVDIKSLRYRSEYIADILYLAD
                     GYHVVMQLDRIKAGKPFVNDPDLNGRLYIKTEDMEHSETEADYAMVHFSLPLGYPLAH
                     ASIFIMGELTGNSLQPEAKMIYNYETMRYEGSLRLKQGYYNYIYVVANHESGAGDTSI
                     IEGNFWETDNEYTILVYHREPGEIFDKLVGYYQSGNEYSPKQ"
     gene            complement(3662..3859)
                     /locus_tag="PK976_00705"
     CDS             complement(3662..3859)
                     /locus_tag="PK976_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66440.1"
                     /translation="MKRRKGSKEVFDGYFKFEGRYIWYYGYKTKGNDTLHLYLEEKLH
                     VEEEKDFLERIENGSKVYTIE"
BASE COUNT         1239 a          815 c          818 g         1378 t
ORIGIN      
        1 ctattccccc tgaaatcaaa ccaaaaatat tcaaagcaac atataggatc catcctcggc
       61 ttacactcac cgcctgtcct attactaacc ttgtaggttt gttcaggcga tctattttct
      121 ggtcgtaaat atcatttact atatagcctg ctgcggtgat tagcacaatt gacagtacga
      181 cgaggaaata tattgtatgg ctgagaggcg aagctaatcc tatagccata taaagcggca
      241 ggaagaagcc ataacgaatg atgaggacga tgagcaatat catcaatagg ttgatccacc
      301 gtaaaaggca tgcataaaca gtgaactggg ttttaattct gcagagtaag gattttccca
      361 taacaatatc tggcttacca gtaaaatgcg tctccattca tccatttccc ttgaagcttc
      421 atcacctgct cgatcacgtc tcttacgcaa cctttgcctc cttctctgtg agaaatgtaa
      481 agagagattg cttttatttc ttcagaggca tcggcagggc agcaaggtaa gccaacctgc
      541 tgcattacgg tgagatcggg aatatcatcc cccatataca aaacaccctg aggttcaata
      601 tgatgaagat gaatatattc attgaagatg gagagtttat cggcaatgcc taagaaaaag
      661 tcatttactt ttagggcatt caggcgatga accatcgaat cactcttgcc tccgctaatt
      721 attacaatcc gatagccatt ctttatggca tgctgaatgg cataaccatc acgcacatat
      781 cctcctctta gaacttcacc atcaggtaga atgaaaattt ctcccgtggt aagcacacca
      841 tcatagtcga acatgaacgt agtaatctga tgaagtaatt ctttataatt cactctgttt
      901 tttctttatt ttttcgtttg gtgatttgtt cagagataat gcgataaata acctcataat
      961 cgggattttc ggaaagcatt cttatatgtt cttcaataat tgaagaatcg cctctgacag
     1021 ccgggccggt ttgggcctgc catggttcca tcaacgtaac cttttcgaat gattcgagta
     1081 taagaggcaa aagaatagag aaaggcaatt tttgttcatt gagaatatcg tgtgctatca
     1141 catacatgta attggtaaag ttgcaagcaa aaacggctga taaatgaatg tgttttcttt
     1201 gagccgaatt ggcttcgata atgttgttcg aaaattgaag ggctaatttt ttaagaatat
     1261 tgaggttctt tttattagag gcctcgatgc aaatcgggat cagcgaaaaa tcggtttcgt
     1321 gatttttggt aaaggtttgt ataggataaa aaacgccatg attagggaaa cgtgacagga
     1381 cttcgatagg tgtcgagcct gaagtatgca taaccaagcc gttggtttgg ggcatggcat
     1441 ccgccacctt tgcaatggct tcatcgttca cgcatataaa ataatattgt gcgtcgggag
     1501 gaattaaggc tgcattcgtc catgactcgc atacatagta ttttgccagt tgccgtgccg
     1561 actgaatcgt acggctgcat attcccctga tggtaaaatt ttttttgtcg agttggtgtg
     1621 ccagatgagt ccctacgttg cctgccccaa taataatcac ctcattctcg gttttttcca
     1681 ttcttcaaaa aatttataca aaagtaattc tttaaaaata tctcatcctt cccttattgc
     1741 tttggactgt attcattacc cgattgataa taacctacaa gtttgtcgaa tatttcacct
     1801 ggttcgcgat gatagaccaa aatggtgtat tcgttgtcgg tttcccagaa gtttccttcg
     1861 atgatggaag tatcacctgc accggattca tgattggcca cgacataaat ataattataa
     1921 tacccttgtt tcaatcgtaa acttccttca tatcgcatag tttcgtaatt ataaatcatt
     1981 ttggcttctg gctggaggga atttcctgta agttcaccca taatgaaaat gcttgcatgt
     2041 gccaatgggt atcccaatgg aagtgaaaaa tgtaccatgg cgtaatcggc ttcagtttcg
     2101 ctgtgttcca tgtcttctgt tttgatgtat aaacgaccgt tgaggtcggg atcgtttaca
     2161 aacggcttgc ctgccttgat tcgatcaagc tgcatcacta catggtaacc atcggcaaga
     2221 tagaggatgt ctgcgatata ttccgaacga tagcgtaaac ttttaatatc gaccgagcga
     2281 tattgattgc ctgcatcgaa taaattctct tcttcataat cataatccaa caagtcgggt
     2341 gtaatattgc ggggctgttt tattgtaagc gcattgtccc aacgccagtt ctggagaatc
     2401 acaatggtga gatccctgtc cggtacagga atcttgtatt tcaccggccg aattgtgaaa
     2461 tcaacttcat gtttggtatt catataagca ggtaaatgtg ctctgccaat tttaacatcc
     2521 tgaattatta ctctgggttc ataaattatg aatcgtaagg aaaactctgg ctgagtaggc
     2581 gaatcgagat aaactacaag aagataattt cctgatttgg tgagcctgat acgatcgttc
     2641 ggaaatatta acgagtaatg cgtataggag gtagtggtgt tgaatgaaaa tttgtaatca
     2701 cgaatctcat ccgagtcgaa tccttccata tactcattca tccttagttg agatggttcc
     2761 caattggcat cgcaatgaag gaaggtgtat tgaaaatttt tgactccacc tgccaaatcg
     2821 tcgaattgca gttgtagcgt tttgtccgaa ttaagcgaaa ttacaggcat ttctaagggg
     2881 taacccgttg gaaagatcaa aacggtttta atgtcgggat tgtatattct gttttgcgtt
     2941 cgtaatgcct tttgactata aaacgctata tcatcctgag ccaatatcga actaaaaaca
     3001 atgattatca atagaataga agcaaatgcg tttttcatac ttgtattttt attatgcgaa
     3061 gataagaaag agcagtgaat acgacgaatt tattcgaaaa aaatctctgt tccaacactt
     3121 actcacttca cttctattat tcggacccct tgattcggct taaatcgaga gttctgaatt
     3181 gtcacaagaa taagtagatc gaaaattatg cttccggaat cgttagatgg tttggaaata
     3241 agtttattta agacctaatt cgcaaaattt acgttttagg cagctacaat tttttcaaaa
     3301 ggttagctcc tcccttggta attttaatgg tattttattt atcgttgatt ttcattcgtt
     3361 tttattctgg ttagaaaatt aatcaagcaa aaagtgaaaa attttccatt gagttcatgt
     3421 tttgtcaaca attgatatat tctataatat cagtgtaagg caatgaaatt gataaacatc
     3481 cagccttcga gtgcaaattc gttttgcatg taactcatat cggcttctac tacgtccttc
     3541 aaggtgtcaa tcatttgctc cgcctctcca cgacttttat attcgagtaa atcttctgtg
     3601 gcgtcttttc agcatttgta agcagagcaa tcgtaccaaa ctctggattc tttttgttga
     3661 atcattctat ggtgtaaact tttgatccat tttctattct ctccagaaaa tccttttcct
     3721 cttcgacatg tagtttttct tccagataca ggtgaagcgt atcattgcct tttgtcttgt
     3781 aaccgtaata ccaaatatac cgaccttcga acttgaaata accatcgaaa acttccttgc
     3841 ttccttttct ccttttcata taattaatct atgagtgaag aaagcctttt caaagagata
     3901 ataaacttta gttaaaacat acaggaaagt cgaatttttt tatttaaaat agaagatggg
     3961 attttggatc gcccatataa aatggctgac gatctttttt gttctgtttg gctaaaagcc
     4021 agatggaaaa acaatgatgg cagtactcag taaaatatta tataatttga tggtttgtca
     4081 tgttgaatca ttaccgaaat ttatggaatt tggatttcga ttgatcaggc cgaccatttt
     4141 ccgggaatag tagctccaca acgaggacaa cggccatttt ctatcaccat tttttttacc
     4201 ttgaatccta tcctttctac taaagaggtt ttgcattttg ggcaaaaggt
//
LOCUS       DAORPD010000032         4234 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_3658_length_4234_cov_2.326633, whole genome shotgun sequence.
ACCESSION   DAORPD010000032 DAORPD010000000
VERSION     DAORPD010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4234
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3658_length_4234_cov_2.326633"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="PK976_00710"
     CDS             complement(<1..445)
                     /locus_tag="PK976_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HRC66441.1"
                     /translation="MFCINNQNTNPYFNLASEEYLLKNYEEDVFMLWRNEPSIIIGKH
                     QNALAEINIEYVKENHIKVVRRITGGGAVFHDLGNLNFTFISNSPNSKPVMNFKKYTQ
                     PIVDVLVNLGVDARFEGRNDIVIDGKKISGNAECVYKNRFLHHGTI"
     gene            639..1832
                     /locus_tag="PK976_00715"
     CDS             639..1832
                     /locus_tag="PK976_00715"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRC66442.1"
                     /translation="MSKSLSEILFEEGEERERYYNAVSPPLIQTSNFVFSTVEQMRRA
                     LSDEYTHSVYSRGNNPTLAILRNKLAKLEGAEDALVFSSGCGAIAAALFSQLRHGDHL
                     VCIRKPYNWVAHFCRIILPKYGIQTTFVEEGSARAYEAACQPNTKLFYLESPNTFTFE
                     ITDIRAVAEIAKSKGIVTVIDNSYSTPLYQSPLSLGIDLVLHSASKYLAGHSDVIAGV
                     LAGSKEMIGKIYNSEYLAFGAVLPPFEAWLMLRGLRTLEIRLQRSSSNAKALIEAIEN
                     HPRIKRILYPFHSQHPKNEIARSQMKDCSGLFSIELEADTPDEVILFCNSLKHFLKAV
                     SWGGFESLVDPAITVYNFDHPELNELSWRTVRFYCGLESPEILIDDVLQALNILDNGA
                     TNRQK"
     gene            1834..2202
                     /locus_tag="PK976_00720"
     CDS             1834..2202
                     /locus_tag="PK976_00720"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HRC66443.1"
                     /translation="MARLLKSFEYAGRGLLEMIRSQMNARIELFILTVVMIAGFVFKI
                     RVNEWITIILVSGLVLAAEAFNSAIENLANAISLRQFDSKIKKIKDLAASGVLISAVC
                     AFVIGLIIFIPYIVRFFVSL"
     gene            2199..3047
                     /locus_tag="PK976_00725"
     CDS             2199..3047
                     /locus_tag="PK976_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HRC66444.1"
                     /translation="MILIVDSGATKAEWRLLLENGDVQAANTTGINPYFIRSESIGNI
                     LMNELPDNWIHESKIGKIYYYGAGCGSEIQEKSIEKALKAIFPEANLFISSDLLGAAR
                     ALWQRDSGYTAILGTGSNAGYFNGQQIEKGIKSLGFILGDEGSGADLGKRLLKRYLEG
                     KFNQDLAENFYQQFPFDLADFLDAIYSKPFPNRFMAGFVPFMKENSNEIEIQLIVEEA
                     FDDFFGLVQTIAQSSFRKSIRFTGSVAWAFEEILLGVAAQHHFEVSLVVKNPIDLLVK
                     FHHAQP"
     gene            complement(3051..3641)
                     /locus_tag="PK976_00730"
     CDS             complement(3051..3641)
                     /locus_tag="PK976_00730"
                     /inference="COORDINATES: protein motif:HMM:NF024724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRC66445.1"
                     /translation="MNNDEKNRLASIVLAPYIQKAYALISISRKTGGNQFRHAMATMS
                     ILIDYHYIDSVLLKAAILHDLLEDFHEAREENIREIQDEDVEEVIRLVKEVTRHFPES
                     KREFYERLLHQGSKKAKILKCADVISNLTDLHIDNISLNKIKTNLNLYEKFILPMALQ
                     VNHNMHLEIEDLIQSRRLYVKSYHKDWFSTLLKRNA"
     gene            complement(3989..4063)
                     /locus_tag="PK976_00735"
     tRNA            complement(3989..4063)
                     /locus_tag="PK976_00735"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4026..4028),aa:Val,seq:tac)
BASE COUNT         1237 a          747 c          873 g         1377 t
ORIGIN      
        1 atatggtgcc atgatgtaaa aaccggtttt tataaacgca ttctgcattt cctgaaattt
       61 tctttccatc gattacgata tcattccggc cttcaaaacg agcatcgact cctaaattaa
      121 ccagaacatc cactatgggc tgagtgtatt ttttgaaatt catcaccggc ttgctattgg
      181 gtgaattcga aatgaaggta aaatttaaat tgcccaagtc gtgaaatact gctcctccac
      241 ccgtgatacg ccttacaacc tttatatgat tttcttttac gtattcgata ttgatctctg
      301 ccagagcgtt ttgatgtttt ccaataataa tagaaggctc attcctccaa agcataaaaa
      361 catcttcctc gtagtttttc agaagatatt cttcgcttgc taaattgaaa taagggttag
      421 tattttgatt gttaatgcaa aacataatta tttagattaa tttttaataa gcagcgaaat
      481 taataaaact tcgaaatatt tgagtctaag tcaaaaaact cgtaaataca agaagtaatt
      541 gaatgagacc ttgataaaac tttgacattc tccgatttta tttggataat tttgtgctcg
      601 atattatcat tttaagaaat gcttcaaaat tttgaattat gtcgaagtcg ctgagtgaaa
      661 ttctgttcga agaaggagaa gaacgtgaaa ggtattacaa tgccgtaagc cctccattga
      721 tacaaacttc caactttgtt ttttcgacgg tagagcaaat gcgtcgtgca ttgagtgatg
      781 aatatacaca ttcggtatat agcagaggaa ataatccaac tttagccatt ttacgtaata
      841 aactggccaa acttgagggc gccgaagatg cgctggtttt ttcgagtggc tgcggtgcca
      901 tagctgcagc tttgtttagc cagttacgcc atggcgatca tctggtttgc attcgaaaac
      961 cctataactg ggtagcgcat ttttgcagga taattttgcc gaaatatggc attcagacta
     1021 ctttcgttga ggaaggcagt gcgcgagctt atgaagctgc ctgccagccc aataccaaat
     1081 tattttatct tgagtcgccc aatactttta cgttcgagat aaccgatatc agagccgtag
     1141 ccgaaatagc caaatcgaaa ggcattgtga cagtcatcga caatagttat tctactcctc
     1201 tttatcagtc gcctttatcc ttagggattg accttgtatt gcattcggca tcgaaatatc
     1261 ttgcgggtca tagtgatgta atagctggag ttttagcggg tagcaaggaa atgattggca
     1321 aaatttataa ttcggaatat ctggcctttg gtgcagtgtt acctccattc gaggcttggt
     1381 tgatgctcag gggcttacga accctcgaga ttcgccttca gcgaagcagt tccaatgcta
     1441 aagcacttat cgaggccatc gaaaaccatc ctcgcataaa aaggattctt tatccttttc
     1501 attcgcaaca tcctaaaaac gaaatcgctc gaagccagat gaaagattgc agcggtttgt
     1561 tttcgatcga attggaggca gatactcccg atgaagtaat tttgttttgc aatagtttaa
     1621 agcatttttt gaaagcggtt tcgtggggag gattcgaatc tttggtcgat cctgctatca
     1681 ccgtttataa ttttgaccat cctgaattaa atgagctctc ctggcgtact gtgcgctttt
     1741 attgcggatt ggaatcacct gaaatattaa tagatgatgt attacaggcc ttgaatattc
     1801 ttgataatgg agcaacaaac aggcaaaagt agaatggcaa ggctgctaaa aagcttcgag
     1861 tatgcaggcc gaggcctctt ggagatgata agaagtcaga tgaatgcgcg aattgaatta
     1921 tttatactca ccgttgtgat gattgcaggt tttgtcttca agattcgggt caatgaatgg
     1981 ataacgataa tcctcgtttc aggcttggtt ttagcggccg aggcattcaa tagcgctata
     2041 gaaaatctgg ccaatgcgat atcgttacgc cagttcgatt ccaagattaa aaagataaaa
     2101 gaccttgcag ccagtggggt tttaatttct gctgtttgtg catttgttat aggtttgatt
     2161 atttttattc cttatattgt tcggtttttt gtatccttat gattttaatc gttgatagtg
     2221 gagctacgaa ggccgaatgg cgtttattgc tcgagaatgg ggatgtgcaa gctgcaaata
     2281 ccacgggaat caatccttat tttattcggt cggaatctat tggtaatatt ctgatgaatg
     2341 aattgcctga taattggatt catgaaagta aaatcgggaa gatttattac tatggtgccg
     2401 gatgcgggag tgagatacaa gaaaaaagta tcgaaaaagc tttgaaagcc atatttccgg
     2461 aggcgaattt gttcatttct tccgaccttt taggagcggc aagagctttg tggcagaggg
     2521 attctggtta taccgctatt ttaggtacgg gttccaatgc aggatatttt aatggtcagc
     2581 aaatcgagaa agggataaaa tcgttgggtt ttatcctggg tgatgaaggc agtggcgctg
     2641 atttggggaa acgcttgctc aaaagatatt tagaaggaaa attcaaccaa gatttagcag
     2701 aaaattttta ccagcaattt cccttcgacc ttgctgattt tttagatgcc atctattcga
     2761 aaccttttcc taatcgtttc atggcagggt ttgttccatt tatgaaggaa aactcgaacg
     2821 aaatagaaat ccagttgatt gtggaggaag cattcgatga tttttttgga ttggtgcaaa
     2881 cgatagcaca atcttctttt cgaaagtcga tacgattcac cggttcggtg gcctgggctt
     2941 tcgaggaaat tttactcgga gttgctgcgc aacatcattt cgaggtgtca ttggtagtaa
     3001 aaaaccctat cgatcttttg gtaaaatttc atcatgctca gccataaata tcaggcattt
     3061 ctttttaaaa gagtagaaaa ccaatcttta tgatatgatt ttacatagag acgcctcgat
     3121 tgaataagat cttcgatttc gagatgcata ttatggttta cttgcaatgc catggggaga
     3181 ataaactttt cgtaaagatt cagattggtt ttaattttat tcaaggaaat attgtcgata
     3241 tgaagatcgg taagattgct gattacgtcg gcacatttca atattttagc ttttttggag
     3301 ccctggtgga gcaacctttc ataaaattct ctttttgatt ctggaaagtg tcgcgttact
     3361 tccttaacaa gccgtatcac ttcttccacg tcttcgtctt gaatctcgcg tatattttct
     3421 tcccttgctt catgaaaatc ttcaagaaga tcgtgaagta tggcagcttt taaaagtaca
     3481 gaatcgatgt aatgataatc gatgaggata ctcatggtag ccatcgcatg cctgaactga
     3541 ttccctccag ttttacggct gatgctaatc aaggcatagg ctttttgaat atatggggcg
     3601 agcacaatag atgcaagcct atttttctca tcgttattca tgaatttttt ctttgtttct
     3661 gataggagaa agtactcttc aatgttctga agatgaatga ataaaaaaag atttaaatag
     3721 ataaaatttt ttatttctat aacttatcta caaaaaatct gccatataat cgatttgtta
     3781 catatggttt agaatcagca ttcgatatag ggaaagggtc gtcgacgcag attgagataa
     3841 attccgaaat cgaatctgcg attttatcat cttatctttt aaaaggcgat cgaatcattc
     3901 cgcttggtta tttcaaagag atgataaaat atactggtat aaaaaaacag gagaatgcct
     3961 atctataaac attctcctgt ttttattgtg ggagctatag gagtcgaacc tatgaccctc
     4021 tgcttgtagg gcagatgctc taaaccaact gagctaagct ccccttcgat attgcgctgc
     4081 aaagatagag cataatcttc gttgtttcca aattttttcg atataattct tggaatttcc
     4141 ttctatggga tgagattttt tgttaattag aagaatatca aattcagaac tcctgatcct
     4201 ataattgcat atcaatattt tacaaaagta ccac
//
LOCUS       DAORPD010000033         4230 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_3663_length_4230_cov_2.259880, whole genome shotgun sequence.
ACCESSION   DAORPD010000033 DAORPD010000000
VERSION     DAORPD010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4230
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3663_length_4230_cov_2.259880"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /locus_tag="PK976_00740"
     CDS             complement(<1..900)
                     /locus_tag="PK976_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HRC66446.1"
                     /translation="MAKRDYYEVLEVSRNATGEEIKKAYRQKAMKFHPDRNPGDKDAE
                     EKFKEAAEAYEVLSDPEKRSRYDQFGHEGVGGAAGGGFSGHGMTMEDIFSNFGDIFES
                     FGGFGGFGGFGGSSRTRSRKTASHGTNLRVKVSLTLEEIAKGAHKKLKVNKYIKCKAC
                     NGTGARAGSSPHVCPTCRGTGQIIQTTNTFLGRMQTTSTCPQCGGEGRIITDKCPECY
                     GDGIVKGEEIVEVDIPAGVTDGMTLSLSGKGNAGPKGGLSGDLLIVIEELPHPDFQRQ
                     GNHLLYELYISIPDAILGTTVEVP"
     gene            complement(931..1608)
                     /locus_tag="PK976_00745"
     CDS             complement(931..1608)
                     /locus_tag="PK976_00745"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HRC66447.1"
                     /translation="MQPQDLKKPEEKNSMNNDHLDENEEQTNNDLHEDRSMQELNDKV
                     TRGKRRNKHGKTSTNESEVIELEAKMKELEQANEKSEEEIAEWKDKFLRLFSEFDNFK
                     KRNARERLELIKSANSSLIVKLLPVLDDFERALDSLNQNDDEVLKPYHDGIELIYNKF
                     YQILEKEGLKPIGNVGETFDTDYHEALTMIPAPNEEMKGKVVEVIEKGYMLHDKVIRF
                     AKVIVGS"
     gene            complement(1764..3089)
                     /gene="murA"
                     /locus_tag="PK976_00750"
     CDS             complement(1764..3089)
                     /gene="murA"
                     /locus_tag="PK976_00750"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014775204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HRC66448.1"
                     /translation="MDYNNKNRQVYRIKGNKTLSGEITPQGAKNEALQVICATLLTPE
                     EVIIHNVPHILDVLRLIELLEGLGMKAKRIGEHSWSFQADDIHPEYMQSREYHQKARA
                     LRGSMMVVGPVLARFGKAYSPRPGGDKIGRRRVDTHFNGLQKLGAKIGENAATHIPFL
                     EASKLKGTYILLDEASVTGTANIVMAATLAEGTTTLFNAACEPYVVQLCKMLNRMGAR
                     ISGIGSNLLTIEGVEHLGSTTHTLMPDMIEVGSFIGLAAMTQSQLIIKNTNYRSLGII
                     PEIFRRLGVETFPDNNDIIVPTKEHYEIESFCDGSIMSISDAPWPGFTPDLISIALVV
                     STQAKGSVLIHQKMFESRLFFVDSLIEMGARIILCDPHRATVIGLDWKYRLRGICMSS
                     PDIRAGVALLIAALSAKGESIIENIEQIDRGYENLDARLQVLGADIERI"
     gene            complement(3094..3717)
                     /locus_tag="PK976_00755"
     CDS             complement(3094..3717)
                     /locus_tag="PK976_00755"
                     /inference="COORDINATES: protein motif:HMM:NF025488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HRC66449.1"
                     /translation="MEYNTTRGPLKMAEYGRNIQKMVEYIIQIPDREERTRAAQALVK
                     CMEQITPEQKDYTDYKRKLWDHLHIISDFRLDVDATYPLPSKEQVELKPQPIPYSDNK
                     IKYRHYGRNVQKMIDYVSTLDTDHPDRKAMIRVIGNSMKMAYLTWNRDNVADDVILEQ
                     MKELSDGKIDTEGIKLAPTNELLQIIRQQQKLQPVAKPGNKKKRKHK"
     gene            complement(3855..>4230)
                     /locus_tag="PK976_00760"
     CDS             complement(3855..>4230)
                     /locus_tag="PK976_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66450.1"
                     /translation="SLNYCEPSRFLDEIEEEFIESGAKKVSWNPPFASVKSPKTIPTK
                     QPDFSKLKKISPEDVNSPLNPDTEELIRCLQTGMQVRHERFGQGKVISLEGEGANKKA
                     TVFFPGIGQKQLLLKFAKLKII"
BASE COUNT         1032 a          936 c          884 g         1378 t
ORIGIN      
        1 aggcacttcg acggttgtac cgagaattgc atcgggtatg ctgatatata attcgtaaag
       61 taggtgattc ccttggcgtt gaaaatcggg gtgagggagt tcttctatca cgatcagcag
      121 gtcgcctgac aggcctcctt taggacctgc attgcctttg ccgctgaggc tcaaagtcat
      181 tccatcggtt acgccagctg gaatatccac ttctacaatt tcttctcctt ttacgatgcc
      241 atcgccatag cattcggggc atttgtcggt aataattcgg ccttctccgc cacactgggg
      301 acatgtagaa gtggtttgca tacggccaag aaaagtattg gtagtttgaa tgatttgccc
      361 tgtacctcgg caggtaggac aaacatgggg cgagcttccg gctctcgctc ctgtgccgtt
      421 gcaagcctta catttaatgt acttattgac tttcagtttc ttatgagccc ctttggcgat
      481 ttcttccaaa gtaagagaaa cctttacccg caaattggtt ccatgactgg cagttttacg
      541 gctacgtgta cgcgagcttc ctccgaatcc cccaaaaccg ccaaaaccac cgaacgattc
      601 aaaaatatca ccaaagtttg aaaaaatatc ctccatcgtc ataccatggc cgctgaaacc
      661 tccgccagca gctccgccaa ccccttcatg cccaaactga tcgtatctac ttcttttttc
      721 gggatcgctt aaaacctcat aagcttctgc tgcttccttg aatttctctt ccgcatcctt
      781 atcacctggg ttgcgatcag gatgaaactt catggctttc tgcctgtagg ctttcttgat
      841 ttcctccccc gtagcattgc gcgaaacttc taaaacttca taataatctc ttttggccat
      901 atataatcta ccttcctttc cttccattct ttaactgcca actatcacct tggcaaagcg
      961 tattactttg tcatgaagca tatagccttt ttcgattact tcgaccacct ttcccttcat
     1021 ttcttcattg ggtgcaggta tcatcgtcag ggcttcatga taatctgtat cgaaggtttc
     1081 acccacgttg ccgataggtt tcaatccttc cttctccaat atttgataga atttattata
     1141 gattaattct ataccatcgt gatagggctt caaaacctca tcatcgtttt gattcaaaga
     1201 atcgagagca cgttcgaaat catccagcac cggcaataat ttgactataa ggctgctatt
     1261 agcgctttta atgagttcca atcgctcacg tgcattacgc ttcttgaaat tatcaaactc
     1321 ggaaaataat cttaagaact tatctttcca ttcggcaatc tcttcttccg atttttcatt
     1381 tgcttgttcc aattctttca ttttagcctc caattctatt acttcggatt cattggttga
     1441 agtcttgcca tgtttattcc tccgtttacc ccgtgttact ttatcattta attcctgcat
     1501 cgaccgatcc tcatggaggt cattgttcgt ttgctcctca ttttcgtcga gatggtcatt
     1561 attcatagaa tttttttctt cgggtttctt tagatcttgt ggttgcatat tatctggatt
     1621 gatcatacac attatctgct tttcaacatt aatttgactc tttattcaaa gtaatttcac
     1681 tggtttatct ttgataacca caggttgttt gcaaaaataa tgcctttgga gagtaaaggt
     1741 caaaatggca gaacgaagcg gatctaaatg cgttcaatgt cagctccgag gacttgcagg
     1801 cgggcatcca aattttcgta acctctgtca atttgctcta tattttcgat aatgctttca
     1861 cctttggcag aaagggcagc tataaggagg gcgactcctg cgcgaatgtc gggtgagctc
     1921 atgcagattc cgcggaggcg gtatttccag tcgagtccta tcaccgtagc tcggtggggg
     1981 tcgcaaagaa taatgcgtgc acccatttcg ataagactat ccacgaagaa taaacgactc
     2041 tcgaacattt tttgatgaat caacacactt cctttggctt gagtggaaac gaccagagca
     2101 atgctgatca aatcgggtgt aaatccaggc cagggtgcat ccgaaatact catgatggat
     2161 ccatcacaga aagattcgat ttcgtaatgt tccttagtag gtacgatgat gtcgttatta
     2221 tcggggaagg tttcgactcc caaacgtcga aaaatctcgg gaatgattcc taatgatcga
     2281 taattggtat ttttaatgat caattgggat tgggtcatgg cagcaaggcc aataaagctt
     2341 cccacttcga tcatgtcggg catcagggta tgggtggtac tgccaagatg ttcaactccc
     2401 tcgatagtca gtaagttgga tcctatgcca ctgatgcgtg cacccatgcg attgagcatt
     2461 ttgcaaagct gaactacgta aggttcgcaa gctgcattga aaagtgtggt agtaccctca
     2521 gccagtgtag ctgccatgac gatattggcg gttccggtta ccgaggcctc gtcgagcaga
     2581 atataagttc cctttagttt actggcttcg aggaaaggga tatgagtggc ggcattttct
     2641 ccaatctttg ctccgagttt ttgaagtcca ttaaaatgtg tatccactct acgacgtcct
     2701 atcttatcgc caccaggcct gggagaataa gccttgccaa atcgtgcgag tacaggccca
     2761 accaccatca tcgagcctct caaggccctg gctttttgat gatactctcg gctttgcatg
     2821 tactcaggat gtatatcgtc ggcttgaaaa ctccatgaat gttctccaat tcttttcgct
     2881 ttcatcccga gtccttcgag cagttcgata agcctcagca catcgagaat atgcgggaca
     2941 ttatggatga tcacttcttc gggagtaagc agggtagcac agatcacctg aagagcctca
     3001 ttcttggctc cttggggtgt tatttctcct gagagtgttt tattgccttt tattctataa
     3061 acttgtcggt ttttattatt atagtccatt gttctatttg tgttttctct ttttcttatt
     3121 tcctggtttg gcaacaggtt gtaatttttg ctgctgcctg attatttgta gtaattcatt
     3181 ggtaggtgcc aatttaatgc cttcggtgtc aatttttcca tccgatagtt ctttcatttg
     3241 ttcaagaatc acatcatcgg ccacattgtc cctattccat gttaagtaag ccattttcat
     3301 actgtttcca atcacacgta tcatcgcttt acggtcggga tggtcggtat caagggtgct
     3361 cacatagtcg atcattttct gcacattacg accataatgc ctgtatttga tcttgttatc
     3421 tgaataggga ataggttgtg gttttagctc aacttgttct ttcgaaggca aaggataagt
     3481 agcatccaca tcgaggcgga aatcggaaat gatatgcaaa tgatcccaga gtttccgctt
     3541 gtagtcggta taatcttttt gttcaggagt aatttgttcc atgcatttta ccagagcctg
     3601 ggctgcccgc gtgcgttctt ccctgtcggg aatctgtatg atatattcga ccatcttctg
     3661 gatgttgcgg ccatattcgg ccattttgag cggacctcga gtagtattgt attccatgtt
     3721 gttttattca gtgaaacgaa aaaaattgat tcaaatgagg ctaatagatg taaaaaattt
     3781 gagttgttct taataaaggg aacgaataat gtctttctta ttgttcggaa gatagtgaga
     3841 gaaatataat ataatcagat gattttcagt tttgcaaatt taagtaaaag ttgtttttga
     3901 ccaattccgg ggaagaagac tgttgccttt ttattggcac cttctccttc gaggctaatt
     3961 acctttcctt gtccaaagcg ttcgtgtcga acctgcatgc cggtttggag gcaacggatc
     4021 aattcttcag tatcggggtt caagggtgag ttcacatcct caggagaaat ttttttaagc
     4081 ttcgaaaaat cgggttgttt agtagggatg gtttttggtg attttacgga tgcaaaaggt
     4141 ggattccatg aaactttttt tgcacccgat tcgatgaatt cttcctcgat ttcatcgaga
     4201 aagcggcttg gttcgcaata attgaggctg
//
LOCUS       DAORPD010000034         4228 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_3667_length_4228_cov_2.597652, whole genome shotgun sequence.
ACCESSION   DAORPD010000034 DAORPD010000000
VERSION     DAORPD010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4228
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3667_length_4228_cov_2.597652"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(236..2536)
                     /locus_tag="PK976_00765"
     CDS             complement(236..2536)
                     /locus_tag="PK976_00765"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 protein"
                     /protein_id="HRC66451.1"
                     /translation="MKKNCLLFFFTFTVSIAVFSQTVISIIPQPVKIEMKSGEFRFGP
                     ETKIMYMDESLMQLAQYARQQWKFWSGWDGEIQQIKHEKLEGNFVLQIIQPAEKELST
                     EGYGIEITSQNVFLRSNTQAGLIYAIQTLNQLMSANPSQVLPACIIIDYPRFRYRGLH
                     LDVARHFMPADFIYRLLDEMAFHKMNVFHWHLVDDQGWRLEIEKYPKLTSIGAWRPDM
                     EDLDWNARPLQCQPTQEMYGGFYTQDEVRKIVKYAADRNITIIPEIEMPAHVMSALAA
                     YPQFSCSGKNLGVPSGSVWPITHIYCAGNDSTFIFLRDVLIEVMEMFPSIYIHIGGDE
                     ADKSEWNKCPKCQQRMQTENLKNVDELQSYFVQCIEKFLNANGRIMIGWDEILEGGLA
                     PNVVVMSWRGEEGGIAAARLKHNAIMTPASHCYFDYYQGDPAVEPLSIGGYIPLKRVY
                     SYEPVPQELSLEESHYILGAQANTWTEYIRTPQHAEYMIFPRLAALAEVVWSPNNLRN
                     WEDFSRRLENQFKCYDKLGIYYARSAFQARIISQPDSVRRCVEISIETEAPYGEIHYT
                     TNGKDPTDSSPVYTGPFALCESATVKAGVFHGGKCMGAIIAKKFSLHKAFGAKMTFQI
                     PSASRYNGNLGDGVWGSTNYRDGNWKGFLGDDAIIIIDLGKPTKIRSIKVDALQASGS
                     WIFFPEKAICELSKDGKEWKSVGEKMNEHSWQEPVPAIQLFGFENLRENVRFVRLKMK
                     NRGTCPLGHAGEGQPAWIFISEVEIL"
     gene            complement(3287..3931)
                     /locus_tag="PK976_00770"
     CDS             complement(3287..3931)
                     /locus_tag="PK976_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66452.1"
                     /translation="MKCILSTIIACMMIIKCIGQEEIKNPVLNKSLNKYVIGFIPSKA
                     NNIFGIAIGPFGSEVICDKPYTKYSHGLNIQIPGQGILQTFYIFNSPFKQVYKNKIID
                     DEFIKKDTSFKRAIHNGLILSLLGTFTDQVNGVSISGWMSLGKNINGVSINLLWNLYY
                     EINGLSIGIFNSTLVMNGIQIGFINRTVKLKGVQVGLWNRNEKRSLPLINWNFK"
BASE COUNT         1317 a          817 c          758 g         1336 t
ORIGIN      
        1 accagcgaga ctaaagtctt agatacaccc agactttttg caatttctgc cagtgagatt
       61 tttttagcca taaactttat ctccgaatgc aaatgtaaaa aattttaact aaaacgtttt
      121 agctaataac aagaaaaaat tatcaatatc atcaatcaaa taaagtaatt caatccgaac
      181 gttagatacg cttttatatt caatgcattc tattggatga aataagaagg aatcattaca
      241 gaatctccac ttcactgatg aaaatccatg cgggttggcc ttcacccgca tgaccaagag
      301 ggcatgtacc gcgatttttc attttcagcc tgacgaaacg aacattttcg cgcaaattct
      361 cgaagccaaa taattgaatt gcaggcacag gttcttgcca tgaatgctcg ttcatttttt
      421 ctcccacgga tttccattcc tttccatcct tcgaaagttc acatattgcc ttttcgggaa
      481 agaatatcca tgagcccgaa gcctgaagag catctacctt aatggaacgg attttcgtcg
      541 gtttgcccag atcgatgatt attatggcat cgtcacccag aaatcctttc cagttgccat
      601 cccggtaatt tgtgcttccc caaacgccat ctcccaaatt tccgttgtaa cgcgaggcac
      661 ttggaatctg aaacgtcatc tttgctccga aagccttatg cagactgaat tttttagcga
      721 tgatagctcc catgcatttt ccaccatgaa agacacctgc cttcaccgta gccgattcac
      781 aaagagcaaa tgggcccgta taaaccggcg agctgtccgt tggatctttt ccattggtgg
      841 tataatggat ttcgccgtat ggggcttccg tttctatgga tatttccacg caacgtctca
      901 cactgtcagg ctggctgatg attcttgcct gaaatgcact tcttgcataa taaattccta
      961 atttatcgta acacttaaac tgattttcga gtcgacggct gaaatcttcc cagttacgaa
     1021 ggttattggg cgaccaaacc acttcagcca aggcggcaag gcgtggaaaa atcatgtatt
     1081 cggcatgctg aggagtacga atatattctg tccaggtgtt ggcctgagca ccaagaatat
     1141 aatgggattc ctccagactg agttcctgag gaacaggttc gtaactataa actcttttca
     1201 gagggatata gcctccaatg gacaggggtt ctacggcagg atcaccctga taataatcga
     1261 agtagcaatg gcttgctgga gtcatgatgg cattgtgctt cagtcgagct gccgctattc
     1321 caccttcctc acctctccat gacatcacaa ccacattggg agccaatcct ccctctaaaa
     1381 tctcatccca acctatcatt atccgtccgt tagcattcag gaatttctct atgcattgaa
     1441 caaaataact ctgaagctca tctacatttt tcaggttctc ggtttgcatt cgttgctggc
     1501 acttcgggca tttgttccat tcagacttgt cggcttcatc gccaccaata tgaatgtaga
     1561 tcgaagggaa catctccatt acttcgataa gcacatctct taggaaaata aaggtagaat
     1621 cgtttcctgc gcaataaata tgtgtaattg gccacacact gcctgaagga acacccaaat
     1681 ttttgcccga gcatgaaaac tgtggataag ccgccagagc cgacatcacg tgggcaggca
     1741 tttctatttc aggaataatg gtgatattgc gatcggcagc atactttacg atttttctca
     1801 cttcgtcctg agtatagaag ccaccataca tctcttgtgt gggttggcat tgtaacggtc
     1861 gagcgttcca gtccaaatct tccatgtcgg gacgccatgc gccaatgctt gtaagtttgg
     1921 gatatttctc gatttcgagc ctccatcctt ggtcatctac caaatgccag tgaaatacat
     1981 tcatcttgtg gaaagccatt tcatcgagca aacgatagat aaaatctgca ggcatgaagt
     2041 gtcgagctac gtcgagatga agtcctctgt aacgaaatcg tggataatca ataatgatgc
     2101 aggcaggcaa tacctgcgaa ggattcgcac tcatgagctg atttaaggtt tgaatggcat
     2161 aaataaggcc agcctgagta ttggaacgta gaaacacatt ttgtgaagta atttcgatgc
     2221 catagccttc agtacttaat tctttttcgg cgggttgtat aatttgcaat acaaaatttc
     2281 cttccagctt ttcgtgttta atctgttgta tttcaccatc ccaacccgac cagaatttcc
     2341 attgctggcg ggcatactgg gcaagctgca tgagcgattc atccatgtac ataattttag
     2401 tttcagggcc aaatcgaaat tctccggatt tcatttctat tttcacaggt tgtggaatta
     2461 tcgaaatcac ggtttgagag aatacggcaa tggaaactgt aaaagtgaag aagaaaagaa
     2521 gacaattttt tttcataagg ctttaagttt tattgaagtc gtatcgacat aaaccgataa
     2581 gataatgagg ttcatttttg acagtttatc agaataaatg aaaatttgga tatcatcgaa
     2641 aggcgcattt ttattattta ggaaaatgtg caaaatacgg aaataaaaca ttacaataaa
     2701 tttttaacac gaaaaaaatt ataattttaa agttaagcca aagttttttc aaattaatag
     2761 aaaaagatat tggagagaga gcttgagaaa accagagttg ttaatcttca tgatcgagct
     2821 tcgaagcaag tattttatga tcaaatgtag caatttttgc caacaaccat tcatttcctc
     2881 agtcaaaagg ctgtgaagtg tattttttaa ttgatgatgt ccattgattt aaaaaaatca
     2941 tttgcttatc attaatctaa atatggttaa ccacagaggg cgatatagtg aatatctcag
     3001 agtaggttga ttcctcttga aaaggaaatg gaagcagatt attttgtaat tttaaaatga
     3061 tatttgaggt taatctggag ctttttcaaa agatataaaa gatgggatta gtattttata
     3121 attgcaagcg gcgttgaaaa gcaacttcag aacatgttcg taaatagaat taaaaaaaga
     3181 aaacttacgg gaaaatttat cattaaaatt gttctttgaa ataaaagttc attaaggggt
     3241 caccatctcc gattgagggg gtcaatagga ctgaaatatc tattatttat ttaaaattcc
     3301 aatttattag tggtaaagat cttttttcat tcctattcca aaggcctact tgcactccct
     3361 ttaatttaac ggtacgatta ataaatccaa tttgaatacc attcataacc agggtactat
     3421 taaatatgcc gatcgaaagg ccattaattt catagtaaag gttccataac aaattaattg
     3481 acactccatt aatattcttg cctaaactca tccatccaga tatagaaacg ccattaactt
     3541 gatccgtaaa agtgcccaat aaggataaaa ttaaaccatt gtgaattgct cttttaaatg
     3601 aggtatcttt tttgataaat tcatcatcaa ttattttatt tttgtatact tgcttaaatg
     3661 gtgagttaaa gatataaaat gtttgtaaaa taccttggcc gggtatttga atattaagtc
     3721 catgtgaata tttagtatat ggtttgtcac aaattacttc tgaaccaaat ggtcctattg
     3781 caattccaaa tatattattt gcttttgagg gaataaatcc aataacatat ttgttcagac
     3841 ttttattcag aaccggattt tttatttctt cttgacctat gcatttaatg atcatcatac
     3901 atgcaataat agtacttaaa atacatttca taataattct ttttaaatta aggtggggag
     3961 ttccctacct taaaattatt ttgagccaac tttaatatga aaaaacattt acaactgtac
     4021 tgccatttct accatgagaa aaactgacag ggtaaacacc ttgcctaact agagtctgtc
     4081 catacagtca tcaaaatttt cagtaaaagt ataaacttct tatgaaagtt tacctttttg
     4141 aagttattct tggtcaattt tataattttt cagtctaaaa ttaagaactt cttttcaaac
     4201 tcctctaaaa attaagtatt ttttatta
//
LOCUS       DAORPD010000035         4222 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_3675_length_4222_cov_2.910007, whole genome shotgun sequence.
ACCESSION   DAORPD010000035 DAORPD010000000
VERSION     DAORPD010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4222
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3675_length_4222_cov_2.910007"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="PK976_00775"
     CDS             complement(<1..229)
                     /locus_tag="PK976_00775"
                     /inference="COORDINATES: protein motif:HMM:NF012274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HRC66453.1"
                     /translation="MSLIINEKKLLGINGLGRIGKLTLWYHLIQRKFDGIVVNIGREV
                     GKSLDDLVQIILNDSTYGRLDHFLYGHSGKKC"
     gene            complement(261..4163)
                     /locus_tag="PK976_00780"
     CDS             complement(261..4163)
                     /locus_tag="PK976_00780"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HRC66454.1"
                     /translation="MTDVCISDFWIYKDLPDKHQVIQFIYEIFDSLLGENLSEENAKR
                     LVYVFLDFFSKNYNSFGEKDKLVFEFIDILKKNYEKRSLSYLSNIGQFKKSLQKAAYN
                     KTTSGPVIDFMRLLIKNNIVFWDTTTRIEEWYRNNSDKMSADYSDVIKSLGKDFFNDY
                     YQKLNEADSWESLCELAFTFSDIIEEFRSKIENFNKATEQFSYIFYLLQCPGMAFHKD
                     FLLMDLNRAIKRISKELNEDECIESIDKLFSLFSNFNKTHMSLILDSILALGKEIINI
                     NNKKIIHYLEDRIIEFGFVSPGITYLDNDWELKVDPNHVKNTRIWLELIEFNPEMMKK
                     LLSALIINLRIGGIFIYDSDLFQKDVSKLLNSKISPIYKQIKQLCRIFPVYYNEIGAE
                     GLIREISTKIDEISYRNDKLIHFLRKQIHTEGNSSHVQITMEIIRFWYDLDKKRLGKI
                     IPPNVLDTIEIKGKWVKGVHQVLRKISQFSGCTLDELLTKEQDDIEIFLSSINHDDKD
                     DLERVALIFELYRLLKEKYRFETTKIIAVLKRYHFITNDDIESLEAHLNQNDPIGALK
                     EIYSLMIKLNEIIFDPEISEGWESIFYKRHVAAGIPSMYGYYRETKFEALGLTFRLEK
                     VASQLVSTIISEINTEYFTAKTLKKIYSILQLLREGLSLDGIYDQGFDSNLKIFQNSL
                     TSESFTINQYINIFQFMEMNIKEIINKYFISPYESLLKIIIPQYVPQSNTMSADALNK
                     LIIRKSEIFYRELLSSAFLIQYLDNFIGALLNNLRNLINNLSDTEIRSMLSYDPDMVI
                     SPLYHETPALDNQAFIGSKAYFLKKLYLNQYPVPPGFVLSTEVFRRVNLILKLPSLNS
                     EIDQFIKTYVTELENMSGLKFGDTKKPLLLSVRSGAAISMPGAMLTLLNVGLNDEITE
                     SLSKCNNFGWTSWDCYRRLLQSWGMAHNLDRNDFDQIMLDYKQKYNVAQKIDFSPEMM
                     REIAFAYKQLLIDNNIYFEEDPFLQLKKAIISVFYSWDTPRAKVYREYMHIAEEWGTA
                     VIVQQMVFGNLHNESGSGVVFTHDVHDPSSGISLSGDFTFLSQGEDIVAGLVNTLPIS
                     EKQRKEFYHKSPFSLETKFPKIYCRLKEIAHELIEIHGFGHQELEFTFETSDPKDLYI
                     LQSRDMRVMKQNSVEVFATSAKKMQLVGCGIGIGNKVLNGVIVFDSHDLQVLKQESPH
                     KNAILVRPDTVPDDIEMIFECEGLLTAKGGATSHAAVTAASLGKIGVVNCDSMIVFEK
                     EKKCIINGNTFTSFDLIAIDGNKGLVYKGNYPIKIQEL"
BASE COUNT         1277 a          765 c          721 g         1459 t
ORIGIN      
        1 gacatttttt ccccgaatgc ccatacaaaa aatgatcgag cctaccgtaa gttgaatcgt
       61 tgagaatgat ttgaacaaga tcatcaagac ttttgcctac ttctctacca atattaacga
      121 caataccgtc gaattttctt tgaatgagat gataccataa ggttagtttt ccaatacggc
      181 cgaggccatt aattcctaat aatttttttt cattgataat tagtgacata acggtttatt
      241 ttggtttatt acaaaattat ctataattcc tgaatcttga taggataatt tcctttgtat
      301 accaaccctt tgtttccgtc gatagcaatg agatcaaatg aagtaaaagt atttccatta
      361 ataatacatt ttttttcttt ttcaaaaacg atcatgctat cgcaatttac tacaccaatc
      421 ttccctaatg atgctgcagt aacggctgca tgtgaagtgg ctcctccttt tgctgttaat
      481 aatccttcgc attcaaaaat catttctata tcatcgggga cagtatctgg tcgtaccaag
      541 attgcatttt tgtgaggact ctcctgtttg agtacttgca gatcatgcga atcgaataca
      601 atgactccat ttaaaacttt gtttccaatg cctattccac atcctacgag ttgcattttt
      661 tttgcggaag tagcaaaaac ctcaacagaa ttttgtttca taactctcat gtctcttgac
      721 tgtaggatgt ataaatcttt ggggtcggag gtttcgaatg taaattctaa ttcttgatgc
      781 ccaaatccat gaatctctat caattcgtga gcaatttctt tgagtctgca ataaattttg
      841 ggaaattttg tttcgagcga gaaggggctt ttgtgataaa attccttgcg ttgcttttcg
      901 ctgatgggta aagtgtttac taatccagca acaatatctt cgccttggct tagaaaagtg
      961 aaatcgccag acaggctgat gcccgaagat ggatcatgaa catcatgggt aaatacgaca
     1021 cctgaaccag attcattgtg tagatttccg aaaaccatct gttgaactat tactgcggtt
     1081 ccccactctt cggcaatgtg catgtattct ctataaacct tagctcgcgg cgtatcccat
     1141 gaataaaata ccgaaataat ggctttcttc aattgtaaga atggatcttc ttcgaagtaa
     1201 atgttattat ctataagtag ttgtttataa gcaaacgcaa tctcacgcat catttccgga
     1261 gaaaagtcga ttttttgagc tacattgtat ttttgtttat aatcgagcat aatctggtcg
     1321 aaatcatttc tatcgaggtt gtgagccatc ccccatgatt gtaaaagacg acgatagcag
     1381 tcccatgaag tccacccaaa attgttgcat ttgctcaaac tttcggttat ttcgtcgttt
     1441 aatccaacat tcaggagagt taacatagct cctggcatag aaatggctgc acctgaacgc
     1501 acactcaata ataatggttt tttggtatct ccaaatttta aacctgacat gttttccagc
     1561 tcagtaacat aagttttgat aaactggtca atttcagaat ttaaggaagg taatttcaag
     1621 atcaaattta ctcttctgaa aacttcggta ctgagaacaa atcctggggg aacagggtat
     1681 tgatttaaat aaagcttttt aaggaaataa gcttttgacc ctataaaagc ctggttgtcc
     1741 aaggccggtg tctcatggta gagtggactt atgaccatat cgggatcgta gcttaacatg
     1801 ctacggattt ctgtatccga gagattgttg attaggtttc tcaaattgtt cagcagagct
     1861 cctataaagt tatccagata ttgaatgagg aaggctgaag agagtagctc cctataaaat
     1921 atctccgatt ttcgtattat cagtttattg agagcatctg cactcatggt attcgattga
     1981 ggtacgtatt ggggaataat aattttcaaa agagattcat aaggactgat aaaatattta
     2041 ttgatgattt ctttgatatt catttccatg aactggaaga tgttgatgta ctgattgatg
     2101 gtgaaacttt cggaagtgag gctgttttgg aatattttta aattggaatc gaatccctgg
     2161 tcgtagattc catctaaact caaaccctct cttaatagct gcaaaatcga ataaatcttt
     2221 tttagtgttt tagcagtaaa atattctgta ttgatttcgg agattatagt cgaaactaat
     2281 tgagaagcca ctttttcgag ccggaaagtg agccccaggg cttcgaactt cgtttcgcga
     2341 taataaccat acatcgaagg aataccagca gctacatgcc ttttataaaa tattgattcc
     2401 caaccttcac taatttccgg atcaaatatt atttcattca attttatcat taacgaataa
     2461 atttctttta aggctccaat agggtcattc tggttcaaat gagcctcaag gctctcgatg
     2521 tcgtcattcg taatgaaatg gtatcgtttt aaaacagcaa ttatcttggt agtttcgaat
     2581 cgatattttt cttttaaaag cctgtacaat tcaaatatca aagccactct ttccaaatca
     2641 tccttatcgt cgtgatttat ggatgaaagg aatatctcta tatcgtcttg ttcctttgtc
     2701 aataattcat cgagggtgca accagagaat tgggaaattt ttctaagaac ttgatgaact
     2761 ccctttaccc atttcccttt tatttctatt gtatcgagta cattgggagg aattattttt
     2821 ccgagtctct tcttatctaa atcataccag aaccttatga tttccatggt tatctgtaca
     2881 tgtgaactat ttccctcggt atgtatctgt ttgcgtagaa aatgtataag cttgtcgttt
     2941 ctataagata tctcgtcaat ttttgtggaa atttcgcgta ttaagccttc cgctcctatc
     3001 tcattgtaat acacagggaa aatacggcat agttgtttaa tttgtttata gataggagat
     3061 attttagagt tgaggagttt ggatacatct ttttgaaaaa gatcggaatc gtaaataaat
     3121 ataccgccaa tacgcaaatt gatgattaaa gcagagagaa gctttttcat catctcgggg
     3181 ttaaattcga tcagttcgag ccatatcctg gtgtttttaa catgatttgg atctaccttt
     3241 aactcccaat cattgtctaa ataggtaatt cccggactaa caaaaccgaa ttcgattatc
     3301 ctgtcttcga gataatgaat tatcttttta ttgtttatat tgatgatttc ctttcccaaa
     3361 gccagaatgg agtctaagat aagactcata tgcgtcttat tgaaattgga aaaaagagaa
     3421 aataatttat cgatggattc gatgcattcg tcttcgttca attccttgct tattcttttg
     3481 atagccctgt tgagatccat caataggaaa tctttatgaa aggccatgcc agggcactgc
     3541 aataaataaa aaatataaga aaattgctcg gtagctttat taaaattttc tattttgctt
     3601 ctgaattctt ctataatatc ggaaaacgta aaagccagtt cacacaatga ttcccatgaa
     3661 tcggcttcgt ttaatttctg ataataatca ttgaaaaagt ccttccctaa tgattttatt
     3721 acatcggaat aatcggccga catcttatca ctattattcc tgtaccattc ttcgattcga
     3781 gttgtagtat cccaaaaaac aatattattt tttatgagta gcctcataaa atcaattaca
     3841 ggtccagagg tcgttttatt ataagctgct ttttgaagac tttttttgaa ctgcccaata
     3901 ttacttaaat agctcaacga tcttttttcg tagttttttt tgagaatatc gataaattca
     3961 aacaccaact tatccttttc gccaaaagaa ttatagtttt tcgagaaaaa atcgaggaaa
     4021 acataaacta agcgttttgc attctcttct gataaatttt cccccaacaa agaatcgaat
     4081 atttcataaa taaattgaat aacttgatgt ttatcgggta aatccttata tatccagaag
     4141 tcacttatac agacatctgt caaaaggtcg atcaattttt tactattatg atctggtttc
     4201 ttgagttcct cgaaaaaatc ct
//
LOCUS       DAORPD010000036         4175 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_3722_length_4175_cov_2.182282, whole genome shotgun sequence.
ACCESSION   DAORPD010000036 DAORPD010000000
VERSION     DAORPD010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4175
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3722_length_4175_cov_2.182282"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="PK976_00785"
     CDS             complement(<1..676)
                     /locus_tag="PK976_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66455.1"
                     /translation="MAIEIKEISGRKEIKKFVCFPFPLYKGNPFWVPPLIFDEVNALD
                     PRKNPSFEFCEAKLWMAYKDGRAVGRIAGIINHRANQIWNERNARFGWLDFIDDFEVS
                     SALFAQVENWAKSMGMKAVQGPLGFSDMDKEGMLIEGFEELGTIATYYNHPYYPIHTE
                     KAGYQKDVDWLEYELNAEVTVPEKFIRVGQIAQERYKLSIVQFKKKKDLLKYAHQIFE
                     VLNESFR"
     gene            complement(688..1842)
                     /locus_tag="PK976_00790"
     CDS             complement(688..1842)
                     /locus_tag="PK976_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HRC66456.1"
                     /translation="MNTIEIKPVKNVKDLQQFVAFPNILYKGDPHYVPVIVEDGIVTL
                     SSKHNPIIKDCKTAYWMAFRDGKMVGRIAGIINNEFIYKWGKRYARFGWMDFIDDPRV
                     AAKLFETVENWAIQNGMEAINGPLGFTDLDTSGLLIKGFEYKATILGRYNYPYYQKYI
                     EDLGYRKDADWIEYQIDIPDAMPRKLEQIAHYVTQRHNLIPHTFKNINEAFPYVNQIF
                     DIVNQIYGLVYGYVPITKEIGDFYISRYKYLLDPELISIITDRADKVIAFGVAMPSYS
                     DVFIKSKGNMLRLKWLLFKGEHKKTDTVDMYYIGVRPGYIRKGISSVLMYEIGEKIIE
                     RGYRYAESNFEYESNFAVRSLWRHFSHRQHKCRRCYQKYFLSSTRSEDSI"
     gene            complement(1867..3054)
                     /locus_tag="PK976_00795"
     CDS             complement(1867..3054)
                     /locus_tag="PK976_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRC66457.1"
                     /translation="MKLLQQKMSKYDIPQKAQKMGVYPYFRVIESEQDTVVKINGRDI
                     LMFGSNSYLGLTNHPMIKEAAKKAIDKYGSGCAGSRFLNGTLDIHIKLEEKLAEFVQK
                     DAAIIFSAGFLVNAGVIPTLTGRDDYIILDELDHASIIEGRRLSFSQAFKYRHNDMSS
                     LEKILKQCKPDKIKLIVVDGIFSMEGDIVDLPGIVKLAKQYNATVMVDDAHSLGVIGK
                     NGSGTASHFGLEEDVDLIMGTFSKSLASIGGFIASDFYTINYLKHNCRSLIFSASIAP
                     AATGSVLAALEIIKNEPERIEKLWENTHYAMKGFKELGFDTGRSETPVIPLYVRDDAK
                     TFMFTRMLLDEGVFVNPVVSPAVPSDSTLIRFSLMATHTYQQIDQALEACQKVGRKLN
                     LID"
     gene            complement(3143..3856)
                     /locus_tag="PK976_00800"
     CDS             complement(3143..3856)
                     /locus_tag="PK976_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRC66458.1"
                     /translation="MSKGKLFLIPAPISINSPFGWDVKTLSIITEMRYFIVEEIRTAR
                     KLLRRVIPSFPIDECTFQILNEHTPPADIPTMLQPMMKGHDMGLMSEGGIPCIADPGA
                     VLVWMAHCKNIQVIPLPGSSSIFLALAASGFNGQNFCFHGYLPVEKIARKQRIIELEQ
                     KAKHQTQIFIETPYRNISLLGSIMQYCQPTTNLCIASGLLSPNEFIKTMEIKQWKAFT
                     PPIHRVPAVFLLGNVYLTI"
     gene            complement(3853..>4175)
                     /locus_tag="PK976_00805"
     CDS             complement(3853..>4175)
                     /locus_tag="PK976_00805"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRC66459.1"
                     /translation="SGMIVPSIHPVSIADGLLTSLGSRTFPVIIENVTEILRVSEEKI
                     VKAMQLIWERMKIIIEPSAAVSFGAVLQYPEIFHSSKTVIILSGGNVDLGCLPFAAIK
                     ENMR"
BASE COUNT         1173 a          857 c          869 g         1276 t
ORIGIN      
        1 gacggaagga ctcgttaaga acctcaaaga tttggtgtgc atatttcagc agatccttct
       61 tctttttgaa ttgtacaata ctgagcttgt agcgttcctg agcaatctgt cctactctga
      121 tgaacttttc gggaacggta acctcggcat tcagttcata ttcgagccaa tcgacatctt
      181 tctgataacc tgctttttca gtatgaatgg gataataggg atgattatag taagtagcaa
      241 tagtccctaa ttcttcgaag ccttctatga gcataccttc cttatccatg tcggaaaatc
      301 ccagtggtcc ttgtacggct ttcattccca tgctttttgc ccaattttcg acctgagcaa
      361 ataacgcaga agatacttca aaatcatcga taaaatcgag ccagccaaaa cgggcattcc
      421 gttcgttcca tatctgatta gccctatgat tgatgattcc ggctatgcgt cccactgctc
      481 gtccatcctt ataggccatc cataatttgg cctcacaaaa ttcgaaggaa ggatttttcc
      541 tcgggtcgag agcattcact tcatcgaaaa taagaggggg aacccagaaa ggattccctt
      601 tgtaaagggg aaatggaaag catacaaact tcttgatttc ttttcgtcct gaaatttctt
      661 tgatttcgat tgccataaga ataagtttta tatgctatct tctgaacgtg tgctacttaa
      721 gaaatatttc tgatagcatc tgcggcattt atgctgacga tgtgaaaaat gtctccaaag
      781 cgatcgcacc gcgaaattgc tttcatattc gaagttgctt tctgcataac gataaccacg
      841 ttcaataatt ttttccccaa tctcatacat caataccgaa ctgattcctt tgcgaatgta
      901 tcccggacga actcctatat aatacatatc gaccgtatcg gtttttttat gttctccttt
      961 aaaaagaagc catttaagac gaagcatatt ccctttcgat ttgatgaaca cgtcgctgta
     1021 tgagggcata gcaaccccaa aggcgattac tttatcggct cgatcggtaa taatggaaat
     1081 taattctgga tcgagaagat atttataacg ggaaatatag aaatcaccaa tttctttggt
     1141 aatagggaca tacccataaa ccaggccata tatttggttt acgatatcga agatttgatt
     1201 gacgtaaggg aaggcttcgt ttatattttt gaaggtatgt ggtatcaaat tatggcgctg
     1261 agtaacgtaa tgagctattt gttccaattt acgaggcatg gcatcgggaa tatcaatctg
     1321 atattcgatc cagtcggcgt cttttctata gcccaagtcc tctatatatt tttgatagta
     1381 gggataattg tatctaccca atattgtggc cttatattcg aaacctttga tgagcaaccc
     1441 tgaggtatcg agatcggtaa agccaagtgg accattgatg gcttccattc cattttgaat
     1501 agcccaattt tctactgttt cgaagagttt ggcggctacc cgagggtcgt cgataaaatc
     1561 catccatccg aaacgagcgt aacgctttcc ccatttgtaa atgaattcat tgttaatgat
     1621 tccggcaata cgtcctacca ttttcccgtc ccgaaaagcc atccaatagg ctgttttgca
     1681 atcttttata attggattgt gtttggaaga aagggttact ataccatctt cgacgataac
     1741 gggtacataa tgaggatctc ctttatagag tatgttgggg aaagcaacga actgttgaag
     1801 atccttgacg ttttttaccg gtttaatctc tattgtattc atggtgaggc atcctaaagg
     1861 ttgacttcaa tctatgaggt tgagctttct gcctactttt tggcaagctt ccaaggcttg
     1921 gtcgatttgt tgataggtat gggtggccat cagcgaaaaa cggatgaggg tcgaatcgct
     1981 gggcacagca ggcgaaacta cgggatttac aaaaactcct tcatcgagca acattcgtgt
     2041 aaacatgaag gtttttgcat cgtctcttac ataaaggggg ataacgggag tttccgacct
     2101 cccagtatcg aaacctaatt ccttgaaccc tttcatggcg taatgggtat tttcccaaag
     2161 cttttcgatg cgttcgggtt cattcttaat gatttcgagt gctgccaaaa cgctacctgt
     2221 agcagcagga gcaatgctgg cgctaaatat caatgaacga cagttgtgct tcaaataatt
     2281 gatggtataa aaatcggaag cgatgaaacc tcctatcgaa gccaggcttt tgctgaatgt
     2341 acccatgatg aggtcaacat cttcttccaa tccgaaatgg ctggcagtgc ccgaaccatt
     2401 tttgccaatc actcctaacg aatgagcatc atcgaccatt accgtagcat tatattgttt
     2461 tgccagtttt acgatgccag gtaaatctac aatgtctcct tccatactga agataccatc
     2521 taccacaata agtttgatct tgtcgggttt acattgtttg aggattttct cgagcgaaga
     2581 catatcatta tggcggtatt taaaagcctg agaaaacgat agacggcgac cctctatgat
     2641 cgaggcatgg tctaattcgt cgaggataat ataatcgtca cgtccagtta aagtgggaat
     2701 gactcctgca ttgactaaaa aacctgcact gaaaataatg gctgcatcct tctgaacgaa
     2761 ttctgctagt ttttcttcca gtttgatatg aatatcaagc gtaccattca aaaatcttga
     2821 accagcgcat ccacttccat atttatctat ggctttcttt gcggcttcct taatcatggg
     2881 atgattggta agtccgaggt aactattcga accaaacatt aatatatccc ttccattgat
     2941 tttcaccact gtgtcctgct ccgattcaat gacccgaaag taaggataca cacccatttt
     3001 ttgggctttc tgtggaatgt cgtacttcga catcttctgc tgtaatagct tcatactgtc
     3061 ttgataatag gttttagtta agattcgaca aaaactggca aatatatgga aactttactt
     3121 aaggcaattt cttattgctc cctcatatcg taagataaac atttccaagt agaaacactg
     3181 cgggtactcg atgaatagga ggggtgaatg ctttccattg ttttatttcc atggttttta
     3241 taaattcgtt gggggagagt aggcctgaag caatgcataa attggtggtg ggttggcaat
     3301 actgcatgat tgaacccaag agagagatat ttcggtaggg cgtttcgata aagatttggg
     3361 tttgatgttt tgctttttgt tccagttcaa ttattcgttg tttacgtgca attttttcga
     3421 cgggcaggta gccatgaaaa cagaaatttt gtccgttgaa acctgatgca gctaaggcta
     3481 aaaaaataga agaagaaccc ggtaaaggga taacctgaat atttttgcag tgagccatcc
     3541 aaactaaaac ggcccccgga tcggcaatgc aaggaattcc tccttcgctc atcaatccca
     3601 tatcgtgtcc cttcatcatg ggttggagca tagtgggaat atctgccggt ggggtatgtt
     3661 cattaaggat ttggaatgtg cattcgtcga tagggaagga gggaatgacc ctgcgcagga
     3721 gttttcttgc ggtgcgaatc tcttctacaa taaaatatcg catctccgtg ataatcgaaa
     3781 gggtttttac atcccagcca aagggcgaat tgatggaaat aggggcggga attaaaaata
     3841 attttccttt actcatctca tattttcttt gatggctgca aagggaaggc aaccaagatc
     3901 gacatttcca cccgaaagga tgatgacagt cttagaactg tggaatattt ccggatattg
     3961 aagaaccgcc ccaaacgaaa cggcagccga cggttctata attattttca ttctttccca
     4021 gatcaattgc attgccttca cgattttttc ttcgctaacc ctcaggattt cagtaacatt
     4081 ttctataata acaggaaaag ttctcgagcc cagtgaggtg agcaaaccat cggcaatgct
     4141 tacgggatga atggaaggga caatcatacc actat
//
LOCUS       DAORPD010000037         4148 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_3754_length_4148_cov_2.423161, whole genome shotgun sequence.
ACCESSION   DAORPD010000037 DAORPD010000000
VERSION     DAORPD010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4148
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3754_length_4148_cov_2.423161"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1899
                     /locus_tag="PK976_00810"
     CDS             <1..1899
                     /locus_tag="PK976_00810"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRC66460.1"
                     /translation="ENLPPISESKPLPEGPFVQETRGEIAALRTYQDLLKNPYDEKLF
                     EYYVTASILKIGLDGSRQPFLPPAMYQNLSYSPDGKYILVSTIQKPFSYNVPYSYFSI
                     KYSIHSSSGEFLNDFYLRPVVEDVLPMFDACEKGRRAIQWRNDRPAELCWVEALDNGD
                     PRIETNARDAIFLQNADFKTPSVKIATTLYRFSGIIWANDQLAVLKEYWYKNRRSLTS
                     FISPAFPMSDKKIVFDLSSEDLYADPGDFLTQRNEFNEWILRFSANNKYLYLEGEGYS
                     PEGNRPFLDEFDVSTFSKRRLWQAEGKSNYESIVRVVDPKQPTMITTIESKYEVPNLF
                     MRTGKKLRQLTFFTSPYGELQSKIKSEKIFYKRNDGTELLGTLYLPATYDPVKEGPLP
                     LLMWAYPREYKDAQRAGQVKDSPHQFVRFHYGSPVFWALRGYAVLDEASFPIIGENAA
                     QPNDTFIEQLVEDARAAIDYLAKRGIVDHERVAIGGHSYGAFMVANLLAHSDLFAAGI
                     ARSGAYNRTLTPFGFQSEERTFWEAPEVYLKMSPFVYADRIKSPLLLIHGEADNNSGT
                     FTLQSERLFAAINGLGGKARLVILPYESHGYSARENILHTLWETDVWLEKYLKNKEME
                     STPRPIQE"
     gene            complement(1890..3131)
                     /locus_tag="PK976_00815"
     CDS             complement(1890..3131)
                     /locus_tag="PK976_00815"
                     /inference="COORDINATES: protein motif:HMM:TIGR03991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sugar nucleotidyl transferase"
                     /protein_id="HRC66461.1"
                     /translation="MRIGIFEDEYYRYFQPLARLRPIFDLRCGIYLLREKTSIRFPNI
                     PTDYFVRDILKSLMQETYASIRINEVVSSSYLFINARLLADSSFYSFLHSAEDGQYIL
                     IDGELAAARLNATHLSLLAFDEEGLLVFPSDDRLKIISYDKPGSKMAHYIWDLISENG
                     KQLIDDFQLSPGSGYCGGQLFEGVHLIKEENIFIGKNSKIYPGVTISAENGQVFIGDE
                     VTIMPNSYIQGPCSLGEKTLIKAGSKIYGNTSIGHNCKIGGEIEGSIIHSFSNKQHDG
                     FLGHAYLGQWVNLGANTNNSDLKNNYSTIKAYANGALTDTGLLFLGLIMGDHSKTGIN
                     TMFNTGTMVGIMCNIFGADFPPKYIPDFSWGGKNGLELYKIENALQTAHIVMSRRNQT
                     LSAEMENLIKYYYNQALLNYS"
     gene            3282..>4148
                     /locus_tag="PK976_00820"
     CDS             3282..>4148
                     /locus_tag="PK976_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010538104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HRC66462.1"
                     /translation="MNENTNLNPNPLVEYLNKPNSEFTKADIIRYIEDNDINMLNFRY
                     VGGDGRLKTLNFVIHDYQHLDTLLSAGERVDGSSLFKDIEAGSSDLYVIPRYRTAFVN
                     PFTEIPTMDILCSYYTKEGTPYEDAPENILRKSHEIFKQRTGLSFEAMGELEYYLIRP
                     RSELYHADDQKGYHESTPFCKGDGLRRQAMLYIAQTGGFIKYGHSEVGNFSLGDFDYE
                     QNEIEFMPVAVEDAADQIVIAKWVLRNLAYRNNVNLTFAPKITVGKAGSGFHIHTRLM
                     KDDENMMIENGQL"
BASE COUNT         1249 a          793 c          870 g         1236 t
ORIGIN      
        1 gaaaatcttc ctccgatatc cgaaagcaaa ccgcttccag aaggcccttt tgttcaagaa
       61 acccggggag aaatagccgc attgcggacc tatcaggatt tgctcaagaa tccatatgat
      121 gaaaagttat tcgagtatta tgttactgct tccattttga aaattggatt agatggttca
      181 cggcagccat ttttacctcc tgccatgtat cagaacctga gctattcgcc cgatgggaaa
      241 tatattctgg tctctaccat tcagaaacca ttctcatata atgttcctta ttcctatttt
      301 tctattaaat attccattca cagcagtagt ggagaattct tgaatgattt ttatttgcgt
      361 ccagtagtcg aagatgtttt gcctatgttt gatgcatgtg aaaaagggcg aagggcaata
      421 caatggcgaa atgacaggcc cgccgagctt tgctgggtcg aagcactcga caatggagac
      481 cccagaatag aaaccaatgc aagagatgct atttttttgc agaatgccga ttttaaaacc
      541 ccatcggtaa aaattgcaac taccctgtat cgtttctcgg ggatcatctg ggcaaacgac
      601 cagttagctg tcctgaagga atattggtat aagaatcgta gaagtttgac gagctttatt
      661 tcgcccgcat ttccaatgtc ggataaaaag attgttttcg atctgtcgtc ggaagatctc
      721 tatgccgatc caggagactt tcttacacag agaaacgaat tcaatgaatg gatactgagg
      781 ttttcggcaa acaataaata tctttatttg gaaggggaag gctatagccc cgaaggcaat
      841 cgtccatttt tagatgaatt cgatgtttca acttttagca agcgacgttt atggcaggcc
      901 gaaggaaaat cgaactacga aagcatcgtc agggtagtcg atcctaaaca accaaccatg
      961 attactacaa tagagagcaa atatgaagtt cccaatcttt ttatgcgcac cggcaagaaa
     1021 cttcgacagc taacattttt taccagtcct tatggtgaac ttcaatcgaa gattaaatcg
     1081 gaaaagattt tttataagag gaatgatgga acagagctat tgggtacgct ttatttgcca
     1141 gctacttacg atccggtcaa ggaagggcct ttgccattat tgatgtgggc ttatcccaga
     1201 gaatataaag atgctcagag agcgggacaa gtgaaagatt ctccccatca attcgtacgc
     1261 tttcattatg ggtcgccggt attctgggca ttgagaggtt atgctgttct cgatgaagca
     1321 agctttccta ttatagggga aaatgctgcc cagcctaatg atacttttat cgaacaattg
     1381 gttgaagatg ctcgtgcagc tatagattat cttgccaaaa gaggaattgt cgatcatgaa
     1441 agggttgcca ttggagggca ttcgtatgga gcattcatgg tagccaatct gctggctcat
     1501 tccgatttat ttgctgccgg gattgctcga agtggagcct acaaccgaac ccttactcca
     1561 tttggtttcc agtcggaaga acgaacattc tgggaagccc ccgaggttta cttaaagatg
     1621 tcgccctttg tatatgccga taggattaaa tccccacttc ttttgattca tggagaggcc
     1681 gacaataatt cgggaacttt taccttgcaa agcgaacgct tgtttgcggc tatcaatggg
     1741 cttggaggga aagcgcgttt ggttattctg ccttacgaaa gccatggata ctctgccagg
     1801 gaaaatatcc ttcatacttt atgggaaacc gatgtctggc tcgaaaaata tttgaagaat
     1861 aaggaaatgg aatcaactcc cagacctatt caggagtaat tcagtaaagc ctgattataa
     1921 tagtatttga taagattttc catttcggcc gaaagggtct gatttcgcct gctcattaca
     1981 atgtgtgcag tttgtaatgc attttcgatt ttgtaaagtt ccaatccatt tttacctccc
     2041 caggaaaaat cgggaatgta tttgggaggg aaatcggctc caaagatatt gcacattata
     2101 cccaccatag ttccagtatt gaacatggta tttattccgg ttttggaatg atcacccata
     2161 atcaagccca gaaaaagcag gccagtatcg gtgagtgcac cattagcata agctttgatg
     2221 gtcgaatagt tgttttttag atcggaatta ttggtgtttg ctcccagatt cacccactgc
     2281 ccaagatagg catgtccaag aaatccatca tgctgtttgt tgctgaaaga atggataatg
     2341 ctaccttcaa tttctccacc tatcttgcag ttatgtccaa tacttgtatt gccgtaaatt
     2401 tttgaacccg ccttaatcag agttttttct ccaagcgagc aaggaccctg aatgtagctg
     2461 tttggcataa tggttacttc atcaccaata aaaacctggc cattttcggc tgaaatagtt
     2521 acaccgggat aaattttcga attttttcct ataaatatat tttcttcttt gatcaggtga
     2581 accccttcga ataattgacc accacaataa ccgctaccag ggctcaactg aaaatcgtcg
     2641 ataagctgtt tcccattttc agaaatcaaa tcccagatat aatgagccat tttagaaccc
     2701 ggcttgtcgt aggaaataat ttttaatcgg tcatcacttg ggaaaaccag tagcccttct
     2761 tcatcgaagg ccagcaggct caaatgagta gcattcaacc tggctgctgc aagctcacca
     2821 tcaattagaa tatattggcc atcttctgct gaatgtagga atgaataaaa cgaagaatcc
     2881 gccaataagc gagcattaat gaataaatac gacgaagaaa ctacttcgtt tatgcgtatg
     2941 gaagcgtaag tttcttgcat cagagatttt aagatatcac gcacaaaata atctgttgga
     3001 atattgggaa atcttatgct ggttttttct ctgagcaggt atattccaca cctcagatcg
     3061 aatattggtc ttaaacgggc taaaggttga aaataccggt aatattcatc ttcaaatatc
     3121 ccaatccgca taaagtataa tttttacaaa tgtaattaca taagtggaaa taagaatgca
     3181 aatttgaaaa attaagtcct caatgagaat ttagatgttt cttatcatag aaagtcctaa
     3241 ttttgctgaa aattcaataa ttttaatata ttttaatcgt catgaatgag aatacgaact
     3301 taaatcccaa tcctcttgta gaatatctta acaagccaaa cagcgaattc acgaaggctg
     3361 atatcattag atatatagag gataatgata ttaatatgtt gaatttcagg tatgttgggg
     3421 gtgatggcag actaaagaca ttaaattttg tgattcatga ttaccagcat ttagatactt
     3481 tgttgagcgc tggtgaaaga gtggatggat caagtttgtt caaggatata gaagcgggtt
     3541 cgagtgattt gtatgttatc ccaagatatc gtacggcttt tgtcaatcca tttaccgaga
     3601 tacccacgat ggatatttta tgttcatatt ataccaagga aggaactcct tatgaagatg
     3661 ctcctgaaaa tatcctgcgt aaatcgcatg aaattttcaa acagcggaca ggcttaagtt
     3721 tcgaagccat gggcgagctt gaatattacc ttattcgtcc acgttccgaa ttatatcatg
     3781 ccgatgatca aaaaggatat catgaatcga ctccattttg caaaggagat ggtttgcgca
     3841 ggcaagccat gctttacatt gctcaaacag gtggttttat caagtatggc cattctgaag
     3901 taggaaactt ttctttggga gattttgatt acgagcagaa tgagatcgaa tttatgcctg
     3961 tggctgttga agatgctgcc gatcaaattg tgatcgccaa atgggtttta cgaaatttag
     4021 catatcgcaa taatgtgaat ttgacttttg caccgaagat tacagttggg aaagccggca
     4081 gtggatttca tattcatacc cgattgatga aggacgatga aaacatgatg attgaaaacg
     4141 ggcaattg
//
LOCUS       DAORPD010000038         4132 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_3770_length_4132_cov_2.162620, whole genome shotgun sequence.
ACCESSION   DAORPD010000038 DAORPD010000000
VERSION     DAORPD010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4132
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3770_length_4132_cov_2.162620"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2003)
                     /gene="rpoB"
                     /locus_tag="PK976_00825"
     CDS             complement(<1..2003)
                     /gene="rpoB"
                     /locus_tag="PK976_00825"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HRC66463.1"
                     /translation="MAHKNIDRINFGTTKLTIQYPDFLDVQLQSFKDFFQLETIPENR
                     VNEGLYKVFSENFPISDARNNFVLEFLDYFIDPPRYSIDECLERGLTYSVPLKAKLKL
                     YCTDPEHEDFETIIQDVYLGMIPYMTPKGTFIINGAERVVVSQLHRSPGVFFGQNYHA
                     NGSILYSARIIPFKGSWMEFTTDINNVLYAYIDRKKKLPVTTLLRAIGYESDRDILAI
                     FDLFQEVKVTKTTAKKLIGQKLAARVVRTWIEDFADEDTGEVSSYERTEVIIERETIL
                     EQAHIDKILEFDVKTVLIHKESPDQMDYSIIFNTLQKDPANSAKEAVEFIYRQLRSAD
                     PPDEETARGIIEKLFFSDKRYDLGDVGRYRINKKLGLDIPDEVKVLTREDIIAIIKYL
                     VAMVNNRAEIDDIDHLSNRRVRTVGEQLYGQFGVGLARMARTIRERMNVRDNEVFTPT
                     DLINAKTLSSVINSFFGTNQLSQFMDQTNPLSELTHKRRISALGPGGLTRDRAGFEVR
                     DVHYTHYGRLCTIETPEGPNIGLISSLCVYAKINNLGFIETPYYKVENGKVLMKEPPI
                     YLSAEEEEKKVIAQASTPIDEEGNLIPDKIKVRYLADYPIVSPQEVDLIDCAPNQIAS
                     IAASLIPFLEHDDANRALMGSNMMRQAVPLLNPEVPIIGTGIE"
     gene            complement(2132..2515)
                     /gene="rplL"
                     /locus_tag="PK976_00830"
     CDS             complement(2132..2515)
                     /gene="rplL"
                     /locus_tag="PK976_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HRC66464.1"
                     /translation="MADLKAFAEQLVNLTVKEVNELAKILKEEYGIEPAAAVAVAAAP
                     GASTGAAQETVEEKTSFDVILKDAGKEKLKVVKLVKELTSLGLKEAKDLVDAAPKPIK
                     EGISKDEAEGLKKSLEEVGAIVEIN"
     gene            complement(2595..3116)
                     /gene="rplJ"
                     /locus_tag="PK976_00835"
     CDS             complement(2595..3116)
                     /gene="rplJ"
                     /locus_tag="PK976_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005791518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HRC66465.1"
                     /translation="MRKEDKSRLIDVLTEQIKESNYFYITDASDLNCEITNQLRRLCF
                     KQNIQMKMVKNSLLKKAMERSGRDFSPLYEVLNGHTAVLFSETGSAPARLIQEFRQKT
                     KNPKPLFKGAYIEESFYIGEEQLDALANLKTKNELIGDIIALLQSPALNVISALQSGG
                     GKLAGIVKTLSER"
     gene            complement(3156..3851)
                     /gene="rplA"
                     /locus_tag="PK976_00840"
     CDS             complement(3156..3851)
                     /gene="rplA"
                     /locus_tag="PK976_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HRC66466.1"
                     /translation="MAQLTRKRKEALAQYNRNKAYTLTEACELVKKITYTKFDASVDM
                     DVRLGVDPRKPNQMVRGMVTLPHGTGKTIRVLVLCTPDKEEEAKAAGADYYGLDEYIN
                     KIKNGWTDIDVVITTPNVMPKVGALGRILGPRGLMPNPKSGTVTMDIATAVKEVKAGK
                     IEFKVDKYGIIHAPIAKVSFDANKIYENAKELLQAIIRLKPAAAKGTYIKSIYLSSTM
                     SPGIQVDPRSITA"
     gene            complement(3907..>4132)
                     /locus_tag="PK976_00845"
     CDS             complement(3907..>4132)
                     /locus_tag="PK976_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009752358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HRC66467.1"
                     /translation="PVVVQLFEAVKVQKGSAEPNRNKIGSVTWDQVHEIAAIKMPDLN
                     CFTIESAMKMVAGTARSVGITITGNPPFAD"
BASE COUNT         1097 a          870 c          848 g         1317 t
ORIGIN      
        1 tcttcgatgc ctgttcctat gataggtacc tcagggttaa gcagaggaac agcctgtcgc
       61 atcatgttcg accccatcaa ggcgcggttg gcgtcgtcat gttcaaggaa aggaatgagc
      121 gaagcagcaa tggatgcaat ctggttaggg gcgcagtcga tcaagtcgac ttcctgtggg
      181 cttacgatag gatagtcggc gagatatcta acttttatct tgtcgggaat caggttccct
      241 tcttcgtcta taggcgtact ggcctgggca ataacctttt tttcctcttc ttcggcactt
      301 aaataaatgg gaggttcttt cattaaaacc ttaccatttt caactttgta atatggagtc
      361 tcgatgaaac cgaggttatt gatcttggca taaacacata gagaagaaat gagtccgata
      421 ttgggtccct caggagtttc gatagtgcac aagcgtccat agtgggtata atgcacgtct
      481 ctaacttcaa aacctgcccg gtcgcgagtc aaacctcccg gtcccaaagc ggaaatcctt
      541 cttttgtgtg taagctccga aaggggattg gtttggtcca tgaactgcga caactgattg
      601 gttccaaaga aagaatttat tacggaggaa agtgttttgg cattaatgag atcagtaggt
      661 gtaaaaacct cattatcacg gacattcatc ctttcgcgta tggtacgtgc catacgtgcc
      721 agcccaacgc cgaattgtcc atataattgt tcacctacag tacgcacacg gcgattactc
      781 aaatggtcaa tatcatcgat ctcagccctg ttgtttacca ttgcaacaag gtatttgata
      841 atggcaataa tatcttctcg ggtgagcact ttaacctcgt caggaatatc cagaccgagt
      901 tttttgttga tgcgatatct tcccacatca cccaaatcat agcgtttgtc ggagaaaaac
      961 agtttttcga tgattcctct ggctgtttct tcatcaggtg gatcggcgct acgcaactga
     1021 cgatagataa actcgactgc ttcttttgcc gaattggcag ggtctttttg caaagtattg
     1081 aagatgatgc tgtagtccat ttggtcggga ctttccttat ggataagtac tgttttgaca
     1141 tcgaattcca gtatcttatc aatatgtgcc tgctcgagta tggtttctct ttcaattatg
     1201 acttccgtac gttcgtaaga agaaacttct ccggtgtctt catcggcaaa gtcttcgatc
     1261 catgttctaa ccacacgtgc agcaagtttc tgccctatca gtttctttgc cgtagttttt
     1321 gtaaccttta cttcctgaaa taggtcgaaa atagctaaaa tatctctatc ggattcatat
     1381 ccaatggctc tgaggagagt agtaacgggt aattttttct ttctatcgat ataggcatag
     1441 agcacgttat tgatatcggt ggtaaattcc atccatgaac ccttgaaagg gatgatacgt
     1501 gccgaataca gaatacttcc attggcgtga tagttttggc cgaaaaatac tcctggcgat
     1561 ctatgcaatt gcgaaacaac tactctttca gctccgttga tgataaaagt tcccttagga
     1621 gtcatgtatg gaatcattcc caaatacaca tcctgaataa tagtttcgaa gtcctcgtgt
     1681 tcgggatcgg tacaatacaa tttcagtttc gccttgaggg gaacactgta agttaagcct
     1741 ctttccaaac attcatcgat agaatatctg ggaggatcga taaaataatc aagaaattcc
     1801 aatacaaagt tgttgcgggc atcagaaata gggaaatttt cggaaaaaac cttataaagc
     1861 ccttcattga cgcggttttc aggaattgtt tccagttgga aaaaatcctt gaacgactgc
     1921 aactgaacat caagaaaatc agggtattgg atggtgagct tcgtggttcc gaagttaatc
     1981 ctgtctatgt ttttatgcgc caaggtatta ggttttttag ttcgaacgga aaatgaatta
     2041 aagaacaata cagtaacatg aaaaaagaga taaaccaccg tccttgtgtg ccaaggacgg
     2101 cagttttcag aggaggaaca aagaatagta tctaattgat ttcaacaatt gcgccaactt
     2161 cttcgagaga cttcttaagt ccttcggctt cgtctttcga gatgccttcc ttaataggtt
     2221 ttggagcagc atcaaccagg tccttagctt ctttaaggcc taagctggta agttccttca
     2281 ccagtttaac cacttttaat ttttcttttc cagcatcttt caaaattaca tcgaaagaag
     2341 ttttttcttc aacggtttct tgtgcagcac cggtcgaagc cccaggagca gcagcaactg
     2401 ccacagcggc agcaggttct attccatatt cttctttaag aatttttgca agttcattga
     2461 cttccttaac agttaggtta actaattgtt ctgcaaaagc tttcagatct gccattttta
     2521 attgagtatt aagtgttttt aaaatttgtt ttacaatttg aaaatttggt tacatagggt
     2581 taaaattcca aatatcacct ttctgagagg gttttaacga ttcccgccaa tttgcctcca
     2641 cccgactgca aagcagaaat cacatttaat gcaggcgact gcaacaaagc gatgatatca
     2701 cctatcaatt cattcttcgt cttcaggttt gccagcgcat cgagctgttc ttcaccaata
     2761 tagaatgatt cttctatata tgctccctta aaaagcggtt tagggttttt ggttttctgc
     2821 cggaattcct gaatgagccg agcgggtgca ctgcctgttt cggaaaacaa aaccgcagta
     2881 tgtccattta gcacttcgta aagagggcta aaatcacgtc cactacgttc catggctttc
     2941 ttcaacaagc tgtttttcac cattttcatc tggatattct gtttgaaaca taaccgcctt
     3001 aactgatttg tgatctcaca attaagatca gaggcatcgg taatatagaa ataattactc
     3061 tccttgatct gttcagtcaa tacgtctatg agtcggcttt tatcttcttt tctcattatg
     3121 catttttttt ggcattttga aatagatttt attcatcagg cagtaatcga acggggatcg
     3181 acttggatgc ccgggctcat tgtgctcgac aaataaatac tcttgatgta ggtgcccttt
     3241 gcggcagctg gtttcaatcg gatgattgcc tgaagcagtt cttttgcatt ttcatagatc
     3301 ttgtttgcat cgaacgaaac tttggcaatt ggagcgtgga tgattccata tttatccact
     3361 ttgaattcga ttttacccgc ttttacttct ttaactgccg tagctatatc catagtaaca
     3421 gtacccgact ttggattggg cataaggccg cgagggccaa gaatcctccc caaagcgcct
     3481 actttaggca ttacattggg tgtagtaata acgacatcaa tatcggtcca gccgtttttt
     3541 attttattga tgtattcgtc cagtccgtag taatcagcgc cagctgcttt agcttcttct
     3601 tccttatcag gtgtacatag caccaataca cgtatggttt tccctgttcc atgtggaaga
     3661 gtaaccatac cacgaaccat ctgattgggt ttacgaggat cgacacctaa gcgtacatcc
     3721 atgtcaaccg atgcatcgaa tttggtataa gtaatctttt tgacgagttc acaggcttcg
     3781 gtcagtgtat aagccttgtt cctgttatat tgtgctaaag cttctttgcg ttttcgtgta
     3841 agttgtgcca ttttttaaaa cttgatggtt tgatgtcaag tgctttattt aattaatgtc
     3901 agaatattaa tcagcaaaag gaggatttcc tgtgatagta attccaacgc tgcgtgcggt
     3961 accggcaacc attttcattg ccgattcaat cgtaaagcaa tttaggtcgg gcatttttat
     4021 tgcagcaatt tcatgtacct gatcccaggt tacgctgcca attttattgc gattgggttc
     4081 tgccgaacct ttctgaactt taactgcttc gaataactgt accactaccg gt
//
LOCUS       DAORPD010000039         4084 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_3827_length_4084_cov_2.590221, whole genome shotgun sequence.
ACCESSION   DAORPD010000039 DAORPD010000000
VERSION     DAORPD010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4084
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3827_length_4084_cov_2.590221"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="PK976_00850"
     CDS             <1..699
                     /locus_tag="PK976_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HRC66468.1"
                     /translation="AMGREAGHLAFGIGASCHYPMIIIPEMFNKTEITIDKILRLIIS
                     AMIKRKLLGISYGGAIISEGVFHFLSDEEILSTGINFTFDEHGHPELGNVSKAHIFNL
                     LLQQRLRQLNIPIKSRPVELGYELRCVRPIGFDLMYCNLLGIGVKRLFDQGYSGCMVT
                     CDAKGDISPLFLKDVADENGKVKPRLVNIESEKVKMVFEWNTQYIEPEDYKEAQKYLP
                     NPEIYDFKKILHWD"
     gene            750..1616
                     /gene="rfbA"
                     /locus_tag="PK976_00855"
     CDS             750..1616
                     /gene="rfbA"
                     /locus_tag="PK976_00855"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007901685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HRC66469.1"
                     /translation="MKGIILAGGSGTRLHPLTLALSKQLMPVYDKPMIYYPLSVLMMA
                     DIREILIISTPNDLPNFERLLGDGSQIGCRFSYAVQEIPNGLAQAFVIGEKFIESDKV
                     CLILGDNIFYGRGLQELLLASNDPEGGIVFAYHVSDPERYGVVEFDENFKAISIEEKP
                     KVPRSNYAVPGLYFYDNSVVKIAKNIKPSARGEYEITDVNRHYLVKGKLKVGLLSRGI
                     AWLDTGTFESLTQASQFVEVIQNRQGLLIGSIEEVAYRRGFITKDQLLELAEPLLKSG
                     YGRYLQRIAETE"
     gene            1635..2621
                     /locus_tag="PK976_00860"
     CDS             1635..2621
                     /locus_tag="PK976_00860"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRC66470.1"
                     /translation="MDTEIKSIEQIHELINKEIESLPFSGYPSNLYDPIAYMMHQNGK
                     RLRPALALIACNMFGGDLKKILRPAIGIEIFHNFTLVHDDIMDQSPIRRNHETVYRKW
                     GTNVAILVGDTMFALAYEFLIQTDEGFLGEILRLFNKTAIQVCEGQQLDIEFENRTDV
                     SIDEYIEMIREKTAVLLGTSLMTGAIVARTPKQQAEQLYQMGIKMGLAFQLMDDILDC
                     YAQQDKFGKSIGNDIMTNKRTYLFLQAYQKANPSQRIELDECFGNNTMPADEKISRVK
                     HIYDDLKVGDDGYALADGYFSDALRLLDELPVEDNRKIGLKTLIERLAVRQV"
     gene            complement(2601..>4084)
                     /locus_tag="PK976_00865"
     CDS             complement(2601..>4084)
                     /locus_tag="PK976_00865"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRC66471.1"
                     /translation="PVNTFSSGWQMRVELAKILLRNPSVILLDEPTNHLDIEAITWLE
                     EWIQNFQGALVVVSHDRAFLDNVTHRTIEISQGKIYDFKSSYSEYVVIREEQLEQQLA
                     AYENQQRQIAQIERFIERFRYKATKARQVQSRIKLLEKMDKIEIDGLDRSSIHFRFPP
                     PPSSGKIVVEIKNLSKKYDNKVVLQNIDFTLVKGEKVAFVGRNGEGKSTLSKIIAAGL
                     DYEGIFRLGYNVKIGYYAQNQSELLDLNKTVYETLDETAKGEIRTRLKSILGSFLFSG
                     EAIEKKVEVLSGGEKARLALAKLLLEPVNLLVLDEPTNHLDMLSKDILKAALLDYSGS
                     MIIVSHDRDFLQGLTHRVIEFKQHHINEYLGDIYDFLSARQIDTLRELDTENKIIQNN
                     QILQQSQNKIEYEKRKAHERKRRKIISEIEKCEEAVFQTDNELAEMEHKLANPAQYQD
                     TINSKDFFNRYDYLKKKSERLMTEWEDLHLMLEELDHSDLTDS"
BASE COUNT         1175 a          814 c          872 g         1223 t
ORIGIN      
        1 gccatgggcc gtgaagccgg acatttggct ttcggcattg gagcctcatg tcattatcct
       61 atgattatca ttcccgagat gttcaataaa accgagataa ctatcgataa aattctgcgt
      121 ttgattatct cagccatgat aaaacgtaaa ctactaggca tatcttacgg tggggctatc
      181 attagcgaag gtgtgtttca tttcctgagc gacgaagaaa ttttgagcac aggcatcaat
      241 tttactttcg atgaacatgg ccatcctgaa ctgggcaatg taagcaaggc acatatcttt
      301 aatctattgc tccagcaaag gcttcgccag ttgaatattc ccatcaagag ccgccctgta
      361 gagctgggtt acgaattgcg atgcgttcgg cctataggct tcgatcttat gtattgcaat
      421 cttttaggaa ttggagttaa acgactgttt gatcagggtt attcgggttg tatggtcact
      481 tgcgatgcaa aaggtgacat ctctccacta tttctgaaag atgtagccga tgaaaatgga
      541 aaagtaaaac ctcgcctggt aaatatcgaa tcggaaaagg taaaaatggt tttcgaatgg
      601 aacacccaat acatcgagcc tgaagattat aaagaagctc aaaagtattt acccaaccct
      661 gaaatatatg attttaaaaa aattctccat tgggattgat catctgtttt taaatgtatg
      721 cttgaaattc acgatttaaa atctcataga tgaaaggaat tattttagct ggcggctcgg
      781 gtacgaggct tcatccgctt acattggccc tgagtaagca attgatgcct gtttatgata
      841 aacccatgat ctattatcca ttatccgtat tgatgatggc cgacatcagg gaaattctca
      901 tcatttccac tcctaatgat ttacccaatt tcgagcgatt gttgggcgat ggttctcaga
      961 taggatgccg gttttcgtat gccgttcagg aaatacctaa tggtttggca caggcttttg
     1021 tcattggaga aaaatttatc gagtccgata aggtttgcct cattttaggc gataatattt
     1081 tttatggacg agggttacaa gaactgcttt tagcttccaa cgatcctgaa ggagggatag
     1141 tttttgccta tcatgtaagc gatcccgagc ggtatggtgt agtagagttc gatgagaatt
     1201 tcaaggccat cagcatcgaa gaaaaaccta aggtaccacg ctcaaactat gctgtgccag
     1261 gtttgtattt ttacgataat tctgtagtga aaatagctaa gaatataaaa ccaagtgcac
     1321 ggggagaata tgaaattaca gatgtaaacc gccattatct cgtgaaaggc aaattaaagg
     1381 taggtttgct aagccgcggt attgcatggc tcgatacagg gacattcgaa tcgctgactc
     1441 aggcttctca gtttgtcgaa gtaatccaaa atcgccaagg tctattgata ggaagtatcg
     1501 aagaagtggc ttaccgtcgc ggatttatca ctaaagatca actccttgag ttagccgaac
     1561 ctctgctcaa aagcggttat ggacgatatt tacaacgaat tgctgaaacc gaataaaact
     1621 caaaatcaac taaaatggac acggagatca agagtataga acaaatacat gaactcatca
     1681 ataaggaaat cgaaagtctc ccctttagcg gttacccttc taacctttat gatcccattg
     1741 cttatatgat gcatcagaat ggtaaaaggc tcagacctgc tctggcttta attgcctgca
     1801 atatgttcgg cggagatttg aaaaaaattc tacgaccggc cataggaatc gagatctttc
     1861 acaattttac attagtacac gatgatatca tggaccagtc gcctatacga cgaaatcatg
     1921 aaaccgtgta taggaaatgg ggtactaatg ttgccattct tgtgggtgat accatgtttg
     1981 ctcttgctta tgaatttctg atccaaaccg atgaaggctt tttaggtgaa attttgcggc
     2041 tgttcaataa aacggctatc caagtatgcg aaggccagca actcgatatc gaattcgaaa
     2101 accgaaccga tgtgagcata gacgagtata tcgagatgat acgggaaaaa accgcagtac
     2161 tgcttggtac ttcactgatg actggagcta tagtggccag aaccccaaaa caacaggccg
     2221 aacagcttta tcagatggga ataaaaatgg gtttagcctt tcagctaatg gacgatatcc
     2281 tagattgtta tgctcaacag gataagttcg ggaaaagtat cggtaatgat attatgacca
     2341 ataagcgcac atatttgttt ctgcaagctt atcaaaaagc caatccatcc caacgcatcg
     2401 aactggatga atgtttcgga aacaatacca tgcccgccga tgaaaaaata tcgagagtaa
     2461 aacatattta tgatgattta aaagtgggtg atgatggtta tgccttggcc gatggatatt
     2521 ttagcgacgc ccttcgcctg ctcgatgaat tgccagttga agacaataga aaaattggat
     2581 tgaaaacact tatcgagcgt ttagctgtcc gtcaggtctg agtggtcaag ctcttcgagc
     2641 ataagatgca aatcttccca ttctgtcatt aagcgttctg actttttttt cagataatca
     2701 tatctgttga agaaatcttt actgttgatg gtatcctgat attgagcagg gttggcaagt
     2761 ttgtgttcca tctcggccaa ctcgttatcg gtttgaaaaa ccgcctcttc gcatttttcg
     2821 atctctgaaa taatttttct ccttttacgc tcgtgtgctt ttcgtttttc gtattctatc
     2881 ttattctggc tttgctgtag gatttgatta ttttggatga ttttattttc agtatcgagc
     2941 tcacggagag tatcaatttg gcgggccgag aggaaatcat agatatcgcc cagatattca
     3001 ttaatatgat gttgtttgaa ttcgataact ctatgggtga gtccctgtaa aaaatccctg
     3061 tcgtgcgaaa caatgatcat ggaaccagaa taatccagca aggcagcttt caaaatatct
     3121 ttcgaaagca tgtcgaggtg gttggtgggt tcatctaaaa caaggagatt tacgggttcg
     3181 agaagcagtt tagccaaggc aagtcgggct ttctcgcctc ctgaaagcac ctcgactttt
     3241 ttttcgatgg cttctccact aaacaaaaag ctccccaata tagatttcaa ccttgttctt
     3301 atttcaccct ttgccgtttc gtctaaggtc tcgtaaacgg ttttgttcag atcgagcagt
     3361 tccgattgat tttgggcata atagccaatt ttaacattgt atcccaggcg aaaaatacct
     3421 tcataatcga ggcctgccgc aatgattttc gataaggtgg atttcccttc cccatttctt
     3481 cccacaaaag ccactttttc gcctttcaca agggtaaaat cgatattttg aagcacaact
     3541 ttgttgtcgt actttttact gagatttttt atttctacaa cgattttgcc acttgaagga
     3601 ggaggcggaa aacggaaatg aatactggat cgatcaaggc cgtcgatctc tattttatcc
     3661 attttttcga gcaacttgat gcgcgactgc acctgacgag ccttggtagc cttataacga
     3721 aaacgctcga taaacctttc gatttgagca atttgcctct gttgattttc ataggctgca
     3781 agctgttgtt caagctgttc ttctcttatt acgacatatt ccgaatagct cgatttaaaa
     3841 tcataaattt ttccctgact gatttcgatg gtgcgatggg ttacattatc gaggaaagcc
     3901 ctatcgtgcg aaaccactac aagagccccc tgaaagtttt gtatccattc ttcgagccac
     3961 gtaattgctt cgatgtcgag gtggttggtg ggttcatcga gaagaatgac cgaaggatta
     4021 cgcagaagaa ttttggccag ttcgactctc atttgccagc cactactaaa agtattcaca
     4081 ggcc
//
LOCUS       DAORPD010000040         4077 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_3836_length_4077_cov_2.437593, whole genome shotgun sequence.
ACCESSION   DAORPD010000040 DAORPD010000000
VERSION     DAORPD010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4077
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3836_length_4077_cov_2.437593"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="PK976_00870"
     CDS             <1..685
                     /locus_tag="PK976_00870"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HRC66472.1"
                     /translation="YTPKCLLKIRNKTILGMILDNLIENQIFQIVIVTGFESAQIQQY
                     VKKNYPQLNIEFVYNSDYEFTNNAYSLLLTQKAIEGHSLLLLDGDIVFDPLIIDKLVH
                     CKQENSLALNKAHKNFLSSEEIKVRITKDNYIVEISKDINPILAAGESAGIQLFSVAT
                     TNKLYQILHQQIDYQQHINEFYESAFEELIHRGEKIYAMDIAPFQSMEIDTETDWRKA
                     NNSFNINIL"
     gene            699..1535
                     /locus_tag="PK976_00875"
     CDS             699..1535
                     /locus_tag="PK976_00875"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HRC66473.1"
                     /translation="MVSNFITISIVGLLAGFIFSVPVAGPISILITSNALKGKFWYSL
                     KAAIGASFADFFYVFISMLGLTHFYEWYKPAIPYILLVGAVFVFVIGFRITKTRLKVE
                     NLNDNSILKSKYHNRGGFRTGLFLNFLNPTLFFGWLSSTFITFSLVASLGFNMGGLED
                     SFQESINAVDHSMLQKPSLFTPSLKDSLSYNAPESVEHEAYTTAKAPVTRWYHYTLSA
                     LYAFFVSIGSITWFYIYVSFLVKNRHRINISTLTNVIRGLGFILIGISIYLLGRGIDL
                     LT"
     gene            1547..2251
                     /locus_tag="PK976_00880"
     CDS             1547..2251
                     /locus_tag="PK976_00880"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HRC66474.1"
                     /translation="MKFAIIAAGEGSRLRKEGLYIPKPMIEIGGMTLIDRLIDRAKRY
                     EFSTACIIVNDIYPELANWFRDKDYGIFIQLIVKSTPGSMHSFYELSQFLRDDDFILT
                     TVDPIYTDESFRYFLEFIDTHPHADGIMAATNYVDDEKPLWIICNNRMQIVEFSDTQK
                     ESSLVSAGFYYFKPLVINFLEKAMQQGMFRMRDFQRFLLKQGCSLIACNVGKVIDIDH
                     TSDILQAELMLKSKNS"
     gene            2409..3041
                     /locus_tag="PK976_00885"
     CDS             2409..3041
                     /locus_tag="PK976_00885"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRC66475.1"
                     /translation="METKQKIIDTAIELFQQNGIKSVTMDWIASEAGVSKRTIYEIFT
                     KKAELLKACMDKITEENLNALHQIRQNSTCFFETIKQLEEFHIQSLQRFHPNFFLDVQ
                     KSFPSLWKKMNDQNDTMKMSEIMYFLKESIMNGLIRADLHITFISRVILELFKLAFSI
                     ELDNDTRIDRKNIYKDLIINYFRGIATDKGLKELEQLEHQEVQFSNSAYN"
     gene            3052..3903
                     /locus_tag="PK976_00890"
     CDS             3052..3903
                     /locus_tag="PK976_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66476.1"
                     /translation="MNLHFAAISRGKRYSPNHITNDARILQLTIEELEKQGHSVTVYD
                     EDEIRPDLIDTPYIFNMIRGTRSLGVLQELEANGALVINSTEAILNCYRKNLATLLPP
                     ANIPIPSSIVIDTYMPLPEIPFASNPFKVWIKRGDVHAIHREDVTTVYSIEELNEIIL
                     EYRQRGIHSAVIQQHVDGPVIKFYAVRNTPFFHWYFHDSTFTMQINEIKLKYLAAKCA
                     ETLKVDVYGGDAIVSPDGKIQIIDLNDWPSFAPVRDEAAKYIAQVIINKAYSHSVELN
                     LHSFPSI"
BASE COUNT         1297 a          803 c          723 g         1254 t
ORIGIN      
        1 ttatactccc aaatgtttgc tcaaaatcag aaacaagacc attttgggaa tgatactcga
       61 caacctgatc gaaaaccaga tttttcaaat cgtaatcgtt accggcttcg aatcggctca
      121 aatccaacaa tatgtaaaga aaaactatcc tcaactcaac atagaatttg tatataattc
      181 cgattatgaa ttcaccaaca atgcttactc tttactactt acccaaaaag ctatcgaagg
      241 acatagctta cttttgctcg acggagatat tgttttcgat cctttgatta tcgataaact
      301 tgttcattgt aaacaagaaa attctctggc tttgaacaag gcccataaaa actttttaag
      361 cagtgaagaa atcaaggtga gaattaccaa agacaattat atcgttgaaa tcagtaaaga
      421 tatcaatccg attttagccg caggagaatc ggcaggtatt cagctttttt cggtagctac
      481 tacaaacaag ctctatcaga ttcttcatca acagattgat tatcaacaac atatcaatga
      541 attttatgaa tcggcattcg aggaattaat ccatcgggga gaaaaaattt atgctatgga
      601 tattgctcct ttccagagca tggaaataga tactgaaacc gattggcgca aagcgaacaa
      661 ttcatttaat atcaatattt tataaatcga attaaaaaat ggtcagcaat tttatcacta
      721 tctctattgt aggtttattg gcgggattca tattttcagt gccggttgct ggccctatca
      781 gtattttgat tacttccaat gctctcaaag gaaaattttg gtatagcctt aaagcagcca
      841 taggagcttc gtttgccgat tttttctatg tttttatctc catgctcggc ttaacccatt
      901 tttacgaatg gtataaacct gctattccat atattctatt ggttggagcg gttttcgtat
      961 ttgtaattgg atttagaatt actaaaacca ggttgaaagt tgaaaacctt aacgataatt
     1021 ctattctgaa aagtaaatac cataatcggg gaggatttcg cacaggattg ttccttaatt
     1081 tcttaaaccc cacacttttt tttggatggc tttcctctac ttttataact ttttcgcttg
     1141 tagcttctct tggttttaac atgggaggat tggaagattc tttccaagaa agcataaacg
     1201 cagttgatca ttccatgtta caaaaaccct ctctatttac accttcctta aaagatagcc
     1261 tttcttacaa tgccccggaa tcggtagaac atgaagccta cactacggct aaagctcccg
     1321 ttacacgctg gtatcattac accttatcgg ccttgtatgc gttttttgta tccattggta
     1381 gtattacatg gttctacatt tatgtctcgt ttttagtaaa aaaccggcat cgcatcaata
     1441 tcagcacact tacaaatgtt atccgcggcc tgggttttat attgataggt atatctatct
     1501 atcttttagg tcgcggtatt gacctactca cttaatctac tcgtcgatga aatttgccat
     1561 tatagcagca ggtgaaggca gtcgcctgcg taaagaaggc ctttacattc ccaagcccat
     1621 gatcgaaatc ggtggaatga cactgataga ccgtttgatc gatcgtgcga aacgatatga
     1681 attttcaact gcctgcatca tcgtaaacga tatctatccc gaactggcga actggtttcg
     1741 agataaggat tacgggatat tcatccagtt aattgtcaaa tcgactccag gttcaatgca
     1801 tagtttttat gaacttagtc agtttcttcg cgatgatgat ttcatcctga ctacagtcga
     1861 tccaatttat accgacgaat cgttcagata ctttctagag tttatcgaca ctcatccaca
     1921 tgccgatggc ataatggctg ccacaaatta tgtagatgac gaaaagccac tttggattat
     1981 ctgcaacaat agaatgcaaa ttgtagaatt ttccgacacg caaaaagagt cgtcgttagt
     2041 gagtgccggt ttttactatt tcaagcccct cgttataaac ttcctcgaga aagcaatgca
     2101 acagggtatg tttcgtatgc gcgatttcca gcggtttctt ttaaaacagg gatgttctct
     2161 gattgcctgc aacgttggca aggtaataga catcgatcat acaagcgata tccttcaagc
     2221 cgaactcatg ttaaaatcaa aaaatagtta aaatttaaaa tggtttatag tatattaaat
     2281 atctaatata gaataatcta acctaattta gcagtcattg aactttagct atacttacct
     2341 atttcgtaaa gaaaactttt caaaatgaaa aagttttgta cttttacgcc tccaatcgtg
     2401 atgatggaat ggaaacaaag caaaagatca ttgataccgc cattgagctg ttccaacaaa
     2461 atggaatcaa atcagttacg atggattgga tagcaagcga agcaggagtt tccaaacgta
     2521 ccatttacga aatttttacc aaaaaggccg aacttttgaa agcctgtatg gataaaatta
     2581 cagaggaaaa cctcaatgca cttcatcaga tccgacaaaa ttccacttgt tttttcgaaa
     2641 ctatcaaaca actcgaagaa tttcacatac aatcgttaca gcgttttcat ccaaattttt
     2701 ttctcgatgt tcagaaatct ttcccaagcc tgtggaaaaa aatgaacgat cagaacgata
     2761 ccatgaaaat gagtgaaatt atgtattttc tgaaggaaag catcatgaat ggcttgatta
     2821 gagccgatct tcacatcaca ttcattagcc gggttatact cgaacttttt aaacttgcct
     2881 tttctattga attagataat gatactcgca tcgatagaaa aaatatttat aaagacttga
     2941 ttattaacta ttttagaggg atagcaactg acaaaggatt gaaagaactc gaacaattag
     3001 aacatcagga agttcaattt tcaaattctg cttacaacta aatcagcgat tatgaatcta
     3061 cattttgccg ccatatcgag agggaaaaga tactcaccca atcatattac caacgatgct
     3121 cggattcttc aattaaccat cgaagaactt gaaaagcagg ggcatagtgt tacggtttac
     3181 gacgaggatg aaataagacc cgatttgatc gacacaccct atattttcaa tatgatacga
     3241 ggcactcggt cgctcggggt attacaggaa ttggaagcca atggcgcttt ggtcatcaat
     3301 tctaccgaag caattctcaa ctgctaccgt aaaaaccttg ccactttatt acctcctgcc
     3361 aatattccca ttccctcctc cattgtgata gatacttata tgcctctgcc cgaaattcct
     3421 tttgcctcca atccattcaa ggtatggata aaacgaggtg atgtccatgc cattcatcgc
     3481 gaggatgtaa ccactgtata cagtatcgaa gaattaaacg aaatcattct cgaatatcga
     3541 caaagaggca ttcacagtgc agtgattcaa caacatgtag atggaccggt tataaaattt
     3601 tatgcagtaa ggaatacacc gttttttcac tggtattttc atgatagtac ctttaccatg
     3661 cagattaatg aaataaaact gaaatatttg gcagccaaat gtgccgaaac cctaaaggtt
     3721 gatgtatatg gaggtgatgc tattgtttcg cccgatggta agattcaaat tattgacttg
     3781 aatgattggc cgagttttgc tcctgtacgt gacgaagctg ctaaatacat cgcacaagta
     3841 attataaaca aagcttattc acactccgta gaactcaact tgcacagttt tccgagtata
     3901 tgaagcaaag cgaattacaa aatactttta aatcatcgga tactgaggaa gcgatcgata
     3961 tttactttta tcgaagatta ggttacaggg tagccttatt ttcggcaaag cacaagatac
     4021 gtccgaatac catcaccatt atcagcatat tttggggtgt tgtggcgggt catcttt
//
LOCUS       DAORPD010000041         3930 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_4019_length_3930_cov_2.451097, whole genome shotgun sequence.
ACCESSION   DAORPD010000041 DAORPD010000000
VERSION     DAORPD010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3930
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4019_length_3930_cov_2.451097"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /gene="greA"
                     /locus_tag="PK976_00895"
     CDS             complement(<1..342)
                     /gene="greA"
                     /locus_tag="PK976_00895"
                     /inference="COORDINATES: protein motif:HMM:NF015414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HRC66477.1"
                     /translation="MSEIQYYTKQGLESLKQQLDYLTTVERSRISQMIAEARDKGDLS
                     ENAEYDAAKEQQGLLELKISKLQNLLLNARIIDDVDIDVHQVSLFTTVKLKNIKTGAI
                     TSYSIVPESEAN"
     gene            complement(444..1508)
                     /locus_tag="PK976_00900"
     CDS             complement(444..1508)
                     /locus_tag="PK976_00900"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HRC66478.1"
                     /translation="MKQIYKYISAIVLAIIIFNPFEEIQAGNKDRSGQAGASELLINP
                     WARSSGWGGVNTASVRGLEAMFGNVAGVAHTGSTELIFAQTQWLKGTDITISAFGLTQ
                     RISESGVLGLSVMSMSIGDIMITRTDLPEGGIGTFSPSYLNVGISYSQAFSSSIYGGV
                     LIRIINESISDASAGGIAIDAGIQYITGDQENIKFGIALRNVGPTISFSGDGFATRVS
                     IPGQETLFTLTERQNKFELPTTMSIGAAYDFLFGGSRFTLAGNFQSNAFSNDLFMLGG
                     EFSFRDYVMLRAGYAYEDGIFEDIESSNKITVSKGFSGGLTVQVPFNKEKGSNFSVDY
                     SYRSTEHFDGTHTIGVRIAL"
     gene            complement(1562..>3930)
                     /locus_tag="PK976_00905"
     CDS             complement(1562..>3930)
                     /locus_tag="PK976_00905"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC66479.1"
                     /translation="APDLTIQELDRELVIYITNKKTSNNHREGYVERDITIKTGDSVY
                     RFEGYQIFQLKDASVSVESIHDPAKARLVFQCDIRNGVTKLVNHYPDQAIGYSVPVVE
                     VAGGDNGISHVFRIREDAFATGDNRLVNHKQYYYLAIAYAYNEFKKYTVEPTGLDGQK
                     KPYLAGRKNIKVYTGIPHKTVNGLVTQSNFNDGPVITRIQGQGNGGMSLELSQATIDE
                     ILSKPMASIDNTYGSPDYPIAYQPIYQPGMGPLNITVIDPLNVKKGRFSLQFIRNVEQ
                     NGVLTNAAWIMKDEEGKIYRSDSTINYYYQQLFLDLGLAVHVYQVLYPGDSLSINNGF
                     IESNLIYADSSRRYLSGVYDNNIPSSPQNWIRAGTYKDSENPKYNDWNMSSSPQKPWD
                     ANEYYEKVIGGMWSPYGLCAYHDQSNEGPAYNVISKNLDPLSKLASVDIVLTPDKSKW
                     TRSVVIEMCSDPKLSEGGVKRFNIRAAPSVNKEGKKADPNSPPSTNPDDPNYISAVGF
                     SWFPGYAINLETGERLNIMFGEDSWLSGHNGRDMLWNPTSTLYENNQPIFGGKHYVYV
                     MQHTQIIIQGDSIYEFPAYDGCRYISLILQRSPENIMKTAVYGSTMWVGMPIMDGTHS
                     ENWLSNDARFRIRVSKPYLRYYSRPLPPGSTDTTNNNFPLYGFSTEGLEPIYNDIVKI
                     QKDLDLINIVPNPYYAYSSYENNALDNRVKFTNLPKKCIITIYNMSGTLIRQFTKDDP
                     TTYLDWDLKNFAGIPVAGGVYIVHVKSDGGERILKWFGALRPIDLNTF"
BASE COUNT         1100 a          831 c          810 g         1189 t
ORIGIN      
        1 attggcttcg ctttcgggaa caatagaata ggatgtaata gcacccgttt tgatattttt
       61 caactttacg gtagtaaaga gggaaacctg atgtacatct atgtcaacat catctattat
      121 acgagcattc agtaagagat tctggagttt cgatatcttt aattccaata atccctgttg
      181 ttctttagct gcgtcgtatt cagcattttc tgataaatcg cctttatcgc gggcttcggc
      241 tatcatctga gaaattctcg acctttccac agtagttagg taatcgagct gttgtttcaa
      301 tgattccagc ccttgttttg tgtaatactg aatttcgctc atgttgtaaa caatttacct
      361 tggttttttg tactaaagaa aagaagaccc tttttgcatg aaagggtcct cttttttgat
      421 gttgcaaaat tactaaaaag tccttacaaa gctatccgaa ctccaatagt atgggtgcca
      481 tcgaaatgct cggtcgatct gtaagaataa tcaactgaga agttcgagcc tttttccttg
      541 ttgaaaggaa cttgaactgt caatccgcca ctgaatcctt tcgaaacagt gattttatta
      601 ctgctttcta tatcttcgaa gataccgtct tcgtaggcat atccagctct gagcataaca
      661 taatctctaa aagaaaattc tcctcccagc atgaacaggt cattactgaa agcgttggat
      721 tgaaaatttc cggcaagggt aaatctcgaa ccaccgaaaa gaaaatcgta agctgctcca
      781 atgctcatgg ttgtgggaag ctcgaatttg ttctgacgtt ccgtcaaggt aaataaagtt
      841 tcttggcctg gaatactcac gcgagttgca aaaccatctc ccgaaaatga gatagttggc
      901 cccacatttc tcagagcaat tccaaacttg atgttttcct gatctcctgt aatgtattgg
      961 atacctgcat ctatggcaat tccaccagcc gaagcatctg aaatagattc attgataatg
     1021 cggatcagaa cacctccata aatgctggag gaaaaagcct gagaataaga aattcctaca
     1081 ttgagataac tgggagaaaa cgtacctatt ccaccttcgg gaaggtcggt tcgggtaatc
     1141 atgatatcgc ctatggacat cgacattaca ctcagtccca aaacacccga ttcactgatt
     1201 ctttgagtca aaccaaaagc tgaaatggta atatcggttc ctttcaacca ttgagtttgt
     1261 gcgaatatca attcagtgct gcccgtgtga gcaacacctg ctacattgcc aaacatggcc
     1321 tcgaggcctc tgaccgaggc cgtattcaca ccaccccagc cgctgctgcg agcccaggga
     1381 ttgataagaa gttctgatgc tccggcttgt ccggaacgat ccttgttgcc ggcctgaatt
     1441 tcctcaaagg ggttaaagat gataatggcc agaactattg cggaaatata tttataaatc
     1501 tgtttcatac cttttgtttt ttgagtgatt aaacgtttag catttatcat atcgttgaat
     1561 tttagaaggt attaagatca ataggccgca aagcaccgaa ccatttcaga attcgctcac
     1621 ctccatcgct tttgacgtga acgatgtaaa cccctcctgc aacgggaatt ccggcaaaat
     1681 tcttcaaatc ccaatcgagg taagtggttg gatcgtcttt agtaaactga cgtataagag
     1741 ttccactcat attgtaaatc gtaataatgc attttttggg caggttggta aatttcaccc
     1801 tgttatccag tgcattgttt tcgtaagaag aataggcata ataaggattg gggacaatat
     1861 tgatcaaatc gaggtccttc tggattttta caatatcatt gtaaataggc tcgagacctt
     1921 cggtcgagaa tccatacaat gggaagttat tattggtcgt atcggtactt cccgggggta
     1981 aaggtctgga ataataacgc aaatacggct tagaaaccct tattctgaaa cgggcatcgt
     2041 tcgaaagcca attttcggaa tgcgtgccat ccatgatagg cattccaacc cacatggtgc
     2101 ttccatatac cgctgttttc atgatgtttt cagggctacg ctgcaatatc aggctgatat
     2161 atctgcatcc gtcgtaagca ggaaattcat agatcgaatc accctgaatg attatttggg
     2221 tatgttgcat gacatataca taatgtttgc caccaaaaat gggttggtta ttttcgtata
     2281 atgtcgaagt aggattccag agcatatctc tcccattatg gccgctcagc cagctatctt
     2341 cgccaaacat aatgttgagt cgttcacccg tttcgaggtt gatggcataa cccgggaacc
     2401 aactaaatcc aactgcagaa atatagtttg gatcatcggg gttagtactg gggggagaat
     2461 tgggatcggc tttcttgcct tccttgttca ccgaaggtgc agctcttatg ttaaaacgtt
     2521 tgacacctcc ctccgaaagt tttggatcgg aacacatttc aataaccaca gatcgggtcc
     2581 atttcgattt gtctggtgtt agcacaatat ctacactggc aagcttcgat aagggatcga
     2641 gattctttga gataacatta tatgcagggc cttcattgct ttgatcatgg taagcgcaga
     2701 ggccataagg cgaccacata cctccgatta ccttttcgta atattcattg gcatcccaag
     2761 gcttttgagg agaactcgac atattccagt cgttatattt gggattttca ctatccttgt
     2821 aagtcccagc acgtatccag ttttgaggac tgctggggat attgttgtcg taaactcccg
     2881 aaagataccg gcgagaactg tcagcataaa tcaaatttga ttcgataaaa ccattgttaa
     2941 tagagagcga atcgcccggg tacaaaacct gataaacatg aacggcaagt cctaaatcga
     3001 ggaaaagctg ttgatagtaa taattgatcg tgctatctga tctataaatc ttcccttctt
     3061 catctttcat gatccaggct gcgtttgtca gcactccatt ctgttcgacg tttcttatga
     3121 attgcaggct gaatctacct ttttttacat tcaagggatc gattacggtg atgttgagag
     3181 gacccatgcc tggctgatat ataggttgat aggcgatggg ataatcggga ctgccataag
     3241 tattgtcaat ggaagccata ggtttggaca aaatctcgtc tattgtggct tgcgaaagtt
     3301 ccaatgacat tccaccattt ccttgaccct gaattctcgt aataactggg ccatcattaa
     3361 aattagattg agtaaccaat ccattgactg tcttgtgagg aatacccgta taaaccttta
     3421 tattcttacg tccagccaga tatggttttt tctgaccatc taatcctgta ggttctaccg
     3481 tatatttctt aaattcatta taagcatagg caatggctaa atagtaatat tgtttgtgat
     3541 tgaccagacg attatctccg gtagcaaacg catcttccct tattctgaat acatgactga
     3601 taccattatc accgccagca acttcaacaa cgggtacgct atagcctata gcctgatcgg
     3661 gatagtggtt gaccagtttg gtaacgccat tacgaatatc gcattgaaat accagtcttg
     3721 ccttagcagg atcgtgtatg ctttcgaccg aaactgaggc atccttcaat tgaaatatct
     3781 ggtatccttc gaaacggtag accgaatcac ctgttttaat ggtgatatcg cgttcgacat
     3841 aaccttcacg atgattgttg gaagttttct tattggtgat gtaaatgaca agttcgcggt
     3901 cgagttcctg aatagtcaaa tcgggagcat
//
LOCUS       DAORPD010000042         3925 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_4026_length_3925_cov_2.780103, whole genome shotgun sequence.
ACCESSION   DAORPD010000042 DAORPD010000000
VERSION     DAORPD010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4026_length_3925_cov_2.780103"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="PK976_00910"
     CDS             complement(<1..662)
                     /locus_tag="PK976_00910"
                     /inference="COORDINATES: protein motif:HMM:NF012449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HRC66480.1"
                     /translation="MRTKIVATLGPASFDKSVIERMTKAGVNVFRLNFSHGDHNILRQ
                     IIHYIRELGPAYHHRLAILGDLQGPKIRIGEVENNAINLRQGQEIQLGGGSGLSKEDI
                     LWINYPALTEDVKPGDQVLLDDGKLSLIVKEKLPSGFLRAEVLVGGILTARKGVNLPD
                     SHLTLSALSEKDLTDLRFAYQNQLDWIALSFVRSSEDILKLRENIQFLGRNWNPGIIA
                     KI"
     gene            complement(959..1951)
                     /locus_tag="PK976_00915"
     CDS             complement(959..1951)
                     /locus_tag="PK976_00915"
                     /inference="COORDINATES: protein motif:HMM:NF013383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HRC66481.1"
                     /translation="MNTENTMKFRKIVIVGSGSIGTAMAQALASNDELDTTILSIEPE
                     VIDEINHFHRNSKYFPGFRLASEVKATADKSVLREADLLFLALPSVAVIPYIRENSSL
                     LPPHVVLINLAKGFDQGGQTISEGLIKEFPFRAGAMKGPSFAKEMINGSPTAFTVGCH
                     DNTLWPLFEELFANTQVYLDFSKDIKGVELLSILKNIYAIAVGIMDAQFNTANMRFLF
                     LTRAFNEMRSIHILFGGKKTTMFKYCGFGDFGLTALNDLSRNRTLGLLIGKGYFTEQI
                     SEKVLLEGKMAVNIFCGQIIHSKHLNTFPIMSGLYHIFSNQAEVPEFVEQMLKK"
     gene            complement(1948..3429)
                     /locus_tag="PK976_00920"
     CDS             complement(1948..3429)
                     /locus_tag="PK976_00920"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HRC66482.1"
                     /translation="MFDKFRLSVKQVFIYSLGNIFSKLVGFVLLPLYSQHISISDYSS
                     LILIEPIWQLLSAVLSFSLPTALLRWLAPEKDPKRQGSMVFTTLMALVFILLAFNLLA
                     FPFNAWLNHRGVYGDSQFKLYLDISFLLVTFDVLNLLVLSLLRFHEKPWQYIILNTLK
                     LTVNLGLNVYFIVGRGMGVEAILLSQLIASFLLNVFSFPFLLKHLVPKFESGPLKEML
                     RYGFPLIFTSISAIILSLGDRYVVQHFLTRSDTGVYGIGYKFGGVINMFIIQSFQLGF
                     LPIAYKMLEDAEAKRFFSRIFTYYFGILMISAFALSIFSHEIVAIFTLGNVDYMRAYV
                     VIPLISFGFVLKGAQTYFSFGLHYVKKTKFNAWIVMLCAIFSIGMNILLVPKMGIYGA
                     ALIMLVSNGLLAIFFYFFSQKFYYIHFEGIRLAKVLITGLSLFAIGYFVPVGNLLASI
                     LFKLLLLIAFPLILYLLKFFNVDEINRLRQIFRSLYIKKSFKE"
     gene            complement(3430..>3925)
                     /locus_tag="PK976_00925"
     CDS             complement(3430..>3925)
                     /locus_tag="PK976_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66483.1"
                     /translation="FSSELLEHLPNEIFQKTIQEFKRISKKYIYITVPNRENPDKLMI
                     RCPECKYIFNRPNHLRSFKSEDFTVLFPEYKKIAEFTFGKPVRYYHPALLRLKRRWIP
                     SISWIPYFWIDQNNRTAICPQCNHKFYYFYKFHPLGFLIDGINVIISPKRPYWLMVLL
                     EKNS"
BASE COUNT         1320 a          749 c          720 g         1136 t
ORIGIN      
        1 tctatcttgg caatgattcc tggattccag ttgcgaccta aaaactgaat attttctctc
       61 aattttaaaa tatcttccga acttctcaca aacgacaagg caatccaatc gagttgattt
      121 tgataggcaa atcgcaaatc tgtcaaatct ttttcgctca gtgccgaaag agttaaatgg
      181 ctgtcgggta aattcactcc tttacgagct gttagaatgc cacccactaa tacctctgcc
      241 cgtaaaaacc ccgaaggaag tttttctttt actatcagac taagcttgcc atcgtcgagt
      301 aatacctgat cgccaggttt aacgtcttca gtcagtgctg gatagtttat ccaaagaata
      361 tcttctttag acaaacccga gccgcctcca agctgaattt cttgaccttg ccgaaggttg
      421 atggcattat tttctacttc tcctatacgg atctttggac cttgtaaatc gcctaaaatg
      481 gccaaacgat gatgataagc aggtcctaat tctcgtatat aatgaattat ctggcgaagt
      541 atgttatgat cgccatgcga gaaattcaac ctgaaaacat ttacacccgc tttggtcatc
      601 ctttcgatta ccgacttgtc gaaagacgca ggacctaagg tagcgactat ctttgtgcgc
      661 atatctttat ggtatataga gtaaaacaaa actaaatgag gccgaatacg agtattttaa
      721 ggtttatgcc gtatcaacga tgagaatttt tcatttttat gtaagggggt caaagttcta
      781 aaaaaacagt catttctgca ccatttatat tttgaacata tatcttgaat cggttacttt
      841 taatgtgctt aatctgcata attcgagcga tatagattaa ccgaaaaata cattgtattg
      901 aaaggcatat tccaattcaa taagccctta gagaggatac ttaacactgt tattgcattt
      961 atttttttaa catctgctca acaaattcag gaacttcggc ttggttgcta aagatatgat
     1021 aaagccctga cataatcgga aaagtattca aatgcttgga atgaataatc tgaccgcaaa
     1081 atatattaac ggccatttta ccttcgagca gtaccttctc ggaaatctgt tcggtaaaat
     1141 aacctttccc gataagcaga cccagtgtac ggtttcgtga aaggtcgttc aatgcggtaa
     1201 gcccaaaatc gccaaaaccg caatacttga acatggtagt tttcttccct ccaaacaaga
     1261 tatgaatgga acgcatttcg ttgaaggcac gagtgagaaa caagaagcgc atattggccg
     1321 tattgaactg ggcatccatt atgcccacgg ctatggcata gatatttttc aagatactca
     1381 atagttcgac ccctttgatg tctttcgaaa aatcgagata tacctgagta ttggcaaaga
     1441 gttcctcaaa aagaggccac aatgtattat catggcatcc cacggtgaaa gctgtaggac
     1501 ttccattaat catttctttt gcgaaagaag gacctttcat agctcctgct cggaatggaa
     1561 actccttgat caatccttcc gaaatggttt gccctccttg atcgaatccc ttagcaagat
     1621 ttattagtac aacatgagga ggcaaaagag aagaattttc acgaatatat gggattacgg
     1681 caaccgatgg cagagctaaa aacaacagat cggcttctct tagcacagat ttatctgcgg
     1741 tggctttcac ttcactggcc aacctaaaac cagggaaata tttcgaattt ctatggaaat
     1801 ggtttatttc atcaattacc tcgggttcaa tggaaagaat agttgtatcc aactcgtcat
     1861 tgctggcaag tgcttgagcc atagcagttc caatagaacc cgagcccact atgactattt
     1921 tcctgaattt catcgtattt tctgtattca ttctttgaac gatttcttga tatagagact
     1981 gcgaaaaatt tgccgtaaac gatttatctc atctacatta aaaaatttca acaaatataa
     2041 aatcaaaggg aaagcaatta acaacaataa tttaaagagt atgctcgcta aaagattccc
     2101 aaccggtaca aaataaccaa tggcaaataa gcttaatccg gtgatgagta cctttgccaa
     2161 acgaatccct tcgaaatgga tataataaaa cttctgcgaa aaaaaataga agaaaatagc
     2221 taacaatcca ttggaaacaa gcatgatgag tgctgctcca taaataccca ttttaggaac
     2281 cagtaaaata ttcattccaa tgctaaaaat tgcacaaagc attacaatcc atgcgttaaa
     2341 ctttgttttt tttacataat gcaaaccaaa agagaagtaa gtttgtgcac ccttgagtac
     2401 aaatccaaaa ctaatgagtg gaataactac atatgctctc atataatcta cattgcccag
     2461 cgtaaatatg gctactattt catgcgaaaa aatggaaaga gcaaatgccg aaatcatcaa
     2521 tattccaaaa taataagtaa aaatacgaga gaagaatctt tttgcttctg catcttcaag
     2581 cattttgtag gcaataggca aaaaacccaa ctgaaacgat tgaatgatga acatgttgat
     2641 aacacctcca aacttgtaac ctattccata aacccctgta tcgcttcgag tgaggaaatg
     2701 ttgtaccaca tagcggtcgc caagagaaag tatgatggcc gaaatgcttg taaatatcaa
     2761 tgggaagcca tatctcaaca tctcttttaa aggacctgat tcaaactttg gtacaaggtg
     2821 ttttaaaaga aacgggaatg aaaaaacatt caaaagaaaa ctggctatga gctgactcaa
     2881 caaaatggct tctaccccca ttccccggcc tacaatgaaa taaacattca atcctaaatt
     2941 tacggtgagt ttaagagtat taagaatgat atactgccaa ggtttctcgt gaaatctcaa
     3001 taaactaaga accaataaat taagtacatc gaaggtgacc agtaagaacg aaatatccag
     3061 atataattta aactgagaat cgccataaac tccacgatga tttaaccatg cattgaaagg
     3121 gaaagctaat aagttaaatg ccagcaatat aaacaccaaa gccatcaatg ttgtaaaaac
     3181 catacttcct tgccgtttag gatccttctc aggagctaac catcttaata atgccgtggg
     3241 aaggctaaac gacagaacag ccgaaagaag ctgccaaatt ggttctatca agatgaggga
     3301 agaataatcg ctaatggaaa tatgttgcga ataaagtggc agtaaaacaa agcctaccag
     3361 tttggaaaat atatttccaa gactatagat aaagacctgt tttaccgata atctaaattt
     3421 gtcgaacatc taactgtttt tttccagtaa aaccattaac cagtaaggtc gtttggggga
     3481 gattatcacg ttgataccgt cgatgagaaa tcctaaagga tgaaatttgt agaaataata
     3541 gaatttatga ttgcattgtg ggcatatagc cgtacgatta ttttgatcta tccaaaagta
     3601 aggaatccat gagatggaag gaatccatct gcgtttgagc ctcaataagg ctggatgata
     3661 atatctcacg ggttttccaa aagtaaattc ggctattttc ttatattcgg ggaaaagaac
     3721 cgtgaaatcc tcgcttttga aagatctgag gtgattgggt cggttgaaaa tatatttaca
     3781 ttcagggcag cgaatcatta atttatcggg gttttcgcgg ttgggtacgg taatatatat
     3841 atattttttt gatattcttt tgaattcttg tattgttttt tggaatattt cattgggaag
     3901 atgttcgagc aattcggatg aaaag
//
LOCUS       DAORPD010000043         3896 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_4059_length_3896_cov_2.500130, whole genome shotgun sequence.
ACCESSION   DAORPD010000043 DAORPD010000000
VERSION     DAORPD010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3896
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4059_length_3896_cov_2.500130"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1703
                     /locus_tag="PK976_00930"
     CDS             <1..1703
                     /locus_tag="PK976_00930"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HRC66484.1"
                     /translation="FIPCVNPDGYEFNHLVSPNGGGMWRKNRRLNPDNSYGVDLNRNY
                     GYMWGYDDLGSSPVPSSETYRGPSAFSEPEIQMIRDFAQLYDFNLVFNYHAYSNTLLY
                     PWGYITDTTAENPIFKNFAFKLTNYNANAYGPASLMLYLVNGNSDDWYYAGQVNQQRA
                     FSFTPEIGNNMQGFWPSIDQIILLCQDQVEANFLAIRFGSRYGEITQHNKLFFSQNQN
                     FVSFHFKRYGLEEGVTYKVSLLPLSNLIESVGQPVYFNHPELLISYSDSISFSVSKDI
                     LPGDEIKILLTLEDDYFTHSDTLTLIFGIPYPIFTDECTTMGNWSSNKWGNNSFVYNS
                     PPSSITDSPVGNYSSNANTSITSTQEFDLTKAKAAVLSFYALWDTERRYDFVQVFASI
                     DQGQHWTALQGKYSSPSSNPLVMDQPVYQGTNLQWVNEEINLSPFTGQKLKIKFALKS
                     NQFINKDGFYFDDISLQIIDKSTGITPSEQNNQLLYTIFPNPANGALHIRPANPHTAQ
                     SVQVSIYNIFGKLFLRQSFPSSIRRMDVNLENLPSGVYFISIEAGVEQVQWEKLLISH
                     "
     gene            1829..3346
                     /locus_tag="PK976_00935"
     CDS             1829..3346
                     /locus_tag="PK976_00935"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005863410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HRC66485.1"
                     /translation="MNHEERFKNIIAHAKEYGFIFPSSEIYDGLAGVYDYGQNGVELK
                     NNIRQYWWKAMVQFHENIVGLDSAIFMHPAIWKASGHLDKFNDPLIDNKDSKKRYRAD
                     NLIEDHMAKIESKIAKEIEKAAKKYGDSFDEEQFVNTNRRVLEYKKQLQEIHHRFNTA
                     MDKNDLIELRQLILDLGIVCPVSGTRNWTEIRQFNLMFNTAMGSTSEDATTVYLRPET
                     AQGIFVNYLNVQKTGRMKIPFGIAQAGKAFRNEIVARQFIFRMREFEQMEMQYFIRPG
                     TELTWYEFWKEERLKWHLSLGFPREKYRFHDHEKLSHYANAACDIEFEFPFGFKELEG
                     IHSRTNFDLNAHQQYSGKKIQYFDPEINESYTPYVVETSIGLDRMFLAIISHAYHEET
                     LDDGSERVVLRIPPILAPYKAAVLPLVAKDGLPEKALEIMDILKYTFMCQYEEKDSIG
                     RRYRRQDAIGTPYALTIDHQTLEDDTVTIRERDTMLQDRVIIDKLPEIIQGKLQI"
     gene            complement(3430..>3896)
                     /locus_tag="PK976_00940"
     CDS             complement(3430..>3896)
                     /locus_tag="PK976_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005468535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRC66486.1"
                     /translation="ETVMELVKDACEERIFPVGRLDRNTTGLLLLTNDGELAKKLTHP
                     THRIRKIYEVELDRPLTKSDMMRIASGIQLEDGVAEVDAIAYVGKGEDRTKIGIELHS
                     GKNRVVRRIFEKLRYHVKKLDRVAFGTLTKKNLPRGHWRYLTEHEVNFLKML"
BASE COUNT         1148 a          845 c          771 g         1132 t
ORIGIN      
        1 ttttcattcc ctgtgtaaat cccgatggtt atgaattcaa ccatctggtg agtccaaatg
       61 gtggaggaat gtggaggaaa aaccggcgcc taaacccgga taacagttat ggtgtagatt
      121 tgaatcgcaa ttatgggtat atgtggggct atgatgatct gggttcgagc cctgtccctt
      181 catccgaaac ttataggggt ccatcggctt tctcggaacc agaaatacaa atgattagag
      241 acttcgctca actttacgac tttaatcttg ttttcaatta tcatgcatat agcaataccc
      301 tgttatatcc ctggggctac attacagaca caaccgccga aaaccctatc tttaaaaact
      361 ttgccttcaa gcttaccaat tataatgcta atgcttatgg accggcaagc cttatgcttt
      421 atctggtgaa tggtaattcg gacgattggt attatgctgg ccaggtgaat cagcaaaggg
      481 ccttttcgtt cactccagag atcggcaaca atatgcaggg attttggcct agcatcgacc
      541 aaatcattct tctatgccag gatcaggtag aagcaaattt tctggcaatt cgtttcggaa
      601 gccggtatgg tgaaattact caacacaaca aattgttttt ctctcaaaac caaaactttg
      661 tcagttttca tttcaaacgt tatggactcg aagaaggagt tacctataaa gttagcctgc
      721 tacccttaag taatctgata gaatcagtag gtcaacccgt ttatttcaac catccggaat
      781 tgctcatctc ttattctgat agcatttcct tttctgtctc gaaggatatt ttaccgggtg
      841 atgaaataaa aattcttttg acgttagaag atgactattt cacgcattcc gacacgctta
      901 ccctgatttt tggtattccc tatccaattt ttaccgacga gtgtacaacg atgggaaact
      961 ggtcatcgaa caaatggggc aacaacagtt tcgtttacaa cagtccacct tcttccatta
     1021 ccgactcgcc tgttggaaat tattcgagca atgccaatac ttctattaca tctactcaag
     1081 aattcgatct aaccaaggcc aaggcggccg tgctttcgtt ttatgctctg tgggatacgg
     1141 aacgtcgtta tgatttcgtt caggtgtttg catcaattga ccaaggtcaa cattggactg
     1201 ctttacaggg aaaatattct tcacctagtt cgaatcctct cgtgatggac caacccgttt
     1261 atcagggaac caacctccaa tgggtgaatg aagaaatcaa tctaagccct ttcacagggc
     1321 aaaaactaaa aatcaaattc gccctcaaat caaatcagtt tattaacaag gatggattct
     1381 atttcgatga tatttcacta caaattatcg acaaatccac aggaataact ccatcagaac
     1441 aaaataatca actcctttac accatatttc ccaatcctgc caatggtgca ctgcatattc
     1501 gccccgccaa tcctcatacc gctcaaagtg tccaagtcag catttataat atttttggca
     1561 aattattttt acgacaatct tttccctctt caatccgccg aatggacgta aacctcgaaa
     1621 acctgccttc aggagtctat tttataagta tcgaggcagg tgttgaacaa gtacaatggg
     1681 agaagcttct tatttcgcac taaaccttgt aattattatt gtccctcgat cgtattgtca
     1741 acccgtgcat ttcgttaatt agtcgaaatg aataagcttg tgttgaaaaa tttaacagaa
     1801 cagtgaaaag ctctattttt gcggcatgat aaatcacgaa gaacgattca aaaacatcat
     1861 agcccatgcc aaagagtatg ggtttatatt tccatcgagt gagatttatg atggtttggc
     1921 cggtgtatat gattatggac agaacggagt agaattaaag aacaacatcc gccagtactg
     1981 gtggaaagcg atggtgcaat ttcacgaaaa tattgtaggc ctcgattctg ccatcttcat
     2041 gcatcctgcg atatggaaag cctcaggtca tctcgacaaa ttcaacgatc ccttgatcga
     2101 caacaaggat tcgaaaaaac gctaccgtgc cgacaatctt atcgaagatc acatggctaa
     2161 gatcgagagc aagatagcaa aggaaataga aaaagctgcc aaaaaatatg gtgacagctt
     2221 cgacgaggaa cagtttgtca ataccaatcg gagggttctc gaatataaaa agcaactcca
     2281 agaaatccat catcgcttca atactgcgat ggataaaaac gatttgatag aacttcggca
     2341 attgatcctc gacttgggaa tagtttgtcc cgtatcgggc acgcgcaact ggaccgagat
     2401 cagacagttt aatctgatgt tcaacacagc catgggttcg actagtgaag atgcgactac
     2461 tgtttattta cggcctgaga cagcacaagg tatattcgtc aattatctga atgttcaaaa
     2521 aacggggcgc atgaagattc ctttcggaat tgcccaggcg ggtaaagcat ttcgcaacga
     2581 aatcgttgca cgtcagttta tttttcgcat gcgcgagttc gagcaaatgg agatgcaata
     2641 ttttattcgt cctggcacag aattgacttg gtatgaattc tggaaggaag aacggctgaa
     2701 atggcatttg tccttaggtt tcccccgtga aaaatatcgt tttcatgatc acgaaaaact
     2761 atcacattat gccaatgctg cctgtgatat agaattcgaa tttccttttg gattcaaaga
     2821 acttgaaggc atccattcgc gcacaaattt cgaccttaac gcacatcagc aatattcagg
     2881 gaaaaagatc caatatttcg atcctgagat caacgagagt tatacccctt acgtcgttga
     2941 aacctccatt ggtttggatc gtatgttcct tgccattatt agccacgctt atcatgaaga
     3001 aaccctggat gatggttcgg aaagggtggt tctgcgtatt cctcctattc ttgccccata
     3061 caaagcggct gtgctcccat tggttgccaa ggatggattg ccagaaaaag ccctcgagat
     3121 aatggatatt ttaaagtata cattcatgtg tcaatacgaa gaaaaagact ccattggccg
     3181 tagatatcgc agacaagatg ctattggtac gccctatgcc ctcaccatcg atcatcaaac
     3241 tcttgaagat gatactgtaa ccatacggga gcgggatact atgttgcaag accgcgttat
     3301 tatagataaa ctacccgaaa ttattcaggg gaagctccag atataagatt ataaatgagt
     3361 gagggctatt ctattatttt ctttgagagc caaaaacaaa gagaaaaact attattcttt
     3421 cattaccgat caaagcattt ttaaaaaatt aacctcatgt tcggtgagat aacgccaatg
     3481 tccacggggg aggttctttt tggttaaggt accaaaggcc acgcggtcga gtttttttac
     3541 atgatatctc aatttctcga agatacggcg tacgaccctg tttttaccgc tatgaagttc
     3601 aattcctatt tttgttcgat cttcgccctt tcccacataa gcaatggcgt caacctcggc
     3661 cactccatct tcgagctgta ttcccgaggc aatacgcatc atgtcacttt ttgtaagtgg
     3721 tctgtctaat tctacttcat aaatcttgcg gattcgatga gttggatggg ttaatttttt
     3781 ggccaattct ccatcattgg tcaatagcaa aaggcctgtc gtattcctgt cgaggcggcc
     3841 aacgggaaaa attctttcct cacacgcatc ttttactaat tccattacag tttccc
//
LOCUS       DAORPD010000044         3882 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_4083_length_3882_cov_2.307290, whole genome shotgun sequence.
ACCESSION   DAORPD010000044 DAORPD010000000
VERSION     DAORPD010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4083_length_3882_cov_2.307290"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2608)
                     /locus_tag="PK976_00945"
     CDS             complement(<1..2608)
                     /locus_tag="PK976_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66487.1"
                     /translation="MKTSPFICFILFLFLAQASFAGTQDTVWLKWKKDTSRNIQATNR
                     ILEELAFENGLSYRKIKLGYVLYHQKQINDSSVGKWKVVLHHITGPTKYLGYDLSDFL
                     LPLYLQAYLQHSFHGIIYTQEVKLEHDTVILEFSPKVNLSTDSLSLHFRNANLDEEIE
                     RQLKRVMDEIKTYRGLCASSLYFENVLRQGYNPHDPRISDILAELIEGIRVKESLDSM
                     FSALILPLEKTDPCNLYNSLKAIDIEIYRHRLAFERLLDTDPLSPNDLTPLSDRIFGF
                     WISWIRHEEFSNPLNNGVIAEVARLDPHGKWIEMAHELLHSALLKMGYTNEDEFLKNL
                     SRQLTKKMLQEGEALNKQTLFIQANIILNNALKIAQAADLEDETQAVESQIKVSVRGL
                     YNTYLKVARDGILNGNIVMGEFYLKKALEYQSENREILLGSQPSKSVYEIFSDVCLDK
                     AIILNKEGKFEQALPFLEKAMEYAGRQSIYLRQQEIKQTLSKTLQEIYRQKVISALSS
                     MQLGNYIEGKRLLDEALTMKKTNATSIESIPDEDSAFHLYQKASLYVVMDSLIQISNE
                     LPLDSLLNATSIILLRYAENLADDSLICKKKLELGKLQFERMMAAVGSLLWDFKIEEA
                     HQLWLNANKMIPQWNLQDCSEINERLNEMAERIEIQECRFARYRLELFEYKVRSAFQE
                     GDYLMGDSIADSALQIKTSVADCPLDDSGIAGLLHQYHSLIEYNRLLEQLTTTYETLL
                     ADSLEKLRLKLYSLISSDSSISREFPLPDTIWLVELHPSEKIINYYFENLKEENRWSD
                     ILNLIEKFRQSGLSADWFETWQENLGHHLARIDKIPAKKEASLEYLDQMLPDRVWYKT
                     LRKAYLKEKLGWR"
     gene            complement(2625..>3882)
                     /locus_tag="PK976_00950"
     CDS             complement(2625..>3882)
                     /locus_tag="PK976_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66488.1"
                     /translation="PHLHFELRETSSQAAVNPLQFGINVKDWIRPTITLLKVYPDDFG
                     SLVNGSPHPIKLNLTGWGPYYRLRNKDTLFIAGNAWFGIKTHDLLNDEPNHNGVKSVE
                     LFIDSIPVFAIEFNRLYFDKGRYINALCDYSEIVLNKQWIIQTRKAPYSRLQNIYRIL
                     LNNGSYAFLPDNIYHLKYVVSDHYGNESILRFVVKGIPKVYAPIRPFVPSHIQFSGDR
                     EASYDTVDFCFWAPIGAFYDTVNFSYKRITSGSAFFSDFHRIHTPTTPIHSSAKISIR
                     PNRLPSRLHHKAIIVKVNQGNKPSAMISKWEGSSLKASIRSFGTYAVLADTLPPVIKN
                     LNFKNGQNIKNLPRLNVRISDDLSGIDSYIGSINGEWILLEYDAKNNLLTYFPDEMLS
                     TGENLLHIRVTDNVGNFSEATYNLVY"
BASE COUNT         1131 a          717 c          835 g         1199 t
ORIGIN      
        1 ctctccatcc gagcttctcc tttaaatagg ctttgcgtaa ggttttatac catactctat
       61 cgggaagcat ttgatcgaga tattcaagtg aagcttcttt tttagctggt attttgtcta
      121 ttctggctaa atggtggccg aggttttctt gccatgtttc gaaccaatca gcgcttaatc
      181 ctgattgacg aaatttttct attagattca atatgtcact ccagcggttt tcttctttaa
      241 ggttttcaaa ataataatta attatttttt cggagggatg aagctcaacc agccagatgg
      301 tatcgggaag tggaaattct ctggagatag aagagtcgga gctaataagg gaatacaatt
      361 ttaagcggag tttttcgagt gaatcggcca gaagtgtttc gtatgtggtg gttaattgct
      421 ctaacagccg attatattca atcagtgaat gatattgatg gagtaatccg gcaatacctg
      481 aatcatcgag cggacaatca gcaactgaag tttttatttg cagggcagaa tcggctattg
      541 aatcacccat taaataatct ccttcctgaa aggccgaacg tactttgtat tcgaaaagct
      601 ctaagcgata tcgggcaaaa cggcattctt gaatctcgat acgctccgcc atttcattca
      661 acctctcgtt tatttcgctg caatcctgta aattccattg aggaatcatt ttattggcat
      721 tcagccataa ttgatgtgcc tcttcgatct tgaaatccca aagtaagctt cctactgcag
      781 ccatcattct ctcgaattga agtttcccta attcgagttt ctttttacag atgagggaat
      841 catccgcaag gttttcagca tatcgtaaaa gaattatgga agtggcattc aacagggaat
      901 cgagaggaag ttcattcgaa atttgaatga gagaatccat gacaacataa agagaggctt
      961 tttgataaag gtgaaatgca gagtcttcat caggaataga ctctattgaa gtagcatttg
     1021 tttttttcat cgtgagtgct tcatcgagta atcttttccc ttcgatataa tttcccaatt
     1081 gcatggagct taatgcggag attacttttt gtcgatatat ttcctgaagc gttttggata
     1141 gtgtttgttt aatttcttgc tgccgtaaat aaatgctttg acgacctgca tattccattg
     1201 ctttttctaa aaaaggcaaa gcctgttcga acttcccttc tttattcaat attatagctt
     1261 tatcgaggca aacgtcggaa aaaatttcat atactgattt agacggttgg ctccctaata
     1321 ggatttcacg gttttcagat tggtactcca gagctttttt cagatagaat tcgcccatga
     1381 caatatttcc atttagaatt ccatcgcggg ccaccttcag ataggtattg tataaccctc
     1441 ttaccgatac tttaatttga gactcgacgg cctgggtttc atcttccaaa tcagcagcct
     1501 gagctatttt cagcgcatta ttcagaataa tattggcttg gataaataga gtttgcttat
     1561 tcaatgcctc gccttcttgt agcatttttt ttgtcaattg tcggcttaga ttttttagaa
     1621 attcatcctc gtttgtataa cccattttca agagagcaga atgaagcagc tcatgcgcca
     1681 tttctatcca tttcccatgt ggatcgaggc gggcaacctc agcgataact ccgttgttca
     1741 gcggattaga aaattcttcg tgccgaatcc atgaaatcca gaaaccaaaa atacggtctg
     1801 acaaaggggt taaatcgttg ggcgataggg gatcggtatc gagtaatcgt tcgaaagcca
     1861 gtctatggcg gtagatttca atatcgatag ctttcagcga gttatacaag ttgcatggat
     1921 cggttttctc caagggaaga attaaggcag agaacatgga atcaagagat tctttcacac
     1981 ggattccttc gatgagctcg gctaaaatat cagaaatacg aggatcgtga ggattgtatc
     2041 cctggcggag gacattttcg aaataaaggc tcgaggcaca aagcccacgg taagtcttta
     2101 tttcatccat cacccttttc agctgccttt ctatctcttc atcgagatta gcatttcgaa
     2161 aatgcagact taggctatct gtcgaaagat taaccttagg cgaaaactct aaaattactg
     2221 tatcatgctc cagtttgacc tcttgggtgt aaattattcc atgaaaagaa tgctgaagat
     2281 aggcttggag gtacaatggc aaaagaaaat cagaaagatc ataaccaagg tattttgtcg
     2341 gaccggtaat atgatggaga acgactttcc atttaccaac cgaagaatca tttatttgtt
     2401 tttgatgata taaaacgtaa cccagtttta ttttacgata gctaagtcca ttttcgaatg
     2461 ccagttcttc gaggattcga ttggttgcct gaatgtttcg ggaagtatcc tttttccatt
     2521 tcagccagac agtgtcctga gttccggcaa acgaagcctg agcaaggaag agaaagagga
     2581 tgaaacagat aaatggagag gttttcattg attgaaagat tgaatcaata aacgagatta
     2641 taagttgctt cgctaaagtt cccaacgttg tcggttaccc tgatatggag taaattctca
     2701 cctgtcgaaa gcatttcgtc gggaaaataa gtgagcaaat tatttttggc atcatattcc
     2761 agtaagatcc attccccatt gatgctgcct atgtatgaat caatgcctga gaggtcgtcg
     2821 gatattctca catttaagcg aggtaaattt tttatgtttt gtccattttt aaaattcaga
     2881 tttttgataa ccggaggcag tgtatcggct aaaactgcat aagtaccgaa tgaacgtatg
     2941 gaagccttaa gggaactgcc ttcccatttg ctaatcatgg cagaaggttt gtttccttgg
     3001 tttactttca caataatggc tttatgatga agacggcttg gtaagcggtt ggggcgaatg
     3061 gaaatttttg ctgaagaatg aataggcgtt gtaggtgtgt ggatgcggtg aaaatcggag
     3121 aaaaatgctg atcctgaggt tattcgttta taagaaaaat ttacggtgtc gtaaaaggct
     3181 ccaatgggcg cccagaaaca gaaatccacg gtgtcatacg aagcttcgcg atctcccgaa
     3241 aattgaatgt gagatgggac gaatggacga atgggagcat aaacctttgg tattcccttc
     3301 actacgaaac gaagaatgga ttcgttgcca taatgatcgc taaccacata tttaagatga
     3361 tagatattat cgggcaaaaa tgcatatgag ccattattta acaagattct atagatattt
     3421 tgaagacggc tgtaaggagc tttacgcgtt tgaatgatcc attgcttgtt gagaacgatt
     3481 tcggaataat cgcataaagc attgatatat cgccctttat cgaaatacaa acgattgaat
     3541 tcgatagcga aaactggaat agaatcgatg aacaattcaa ctgatttcac gccgttatga
     3601 ttaggttcgt cattcagaag atcgtgggtt ttaattccga accaagcatt tccagcaata
     3661 aacaaagtat ctttgttacg aaggcgataa tagggccccc aacctgtgag gtttagcttt
     3721 atggggtgag gagaaccatt gactagtgaa ccaaaatcgt cgggataaac tttcagcagg
     3781 gtaatagtag gccgaatcca gtctttcacg tttattccaa attgtaaagg attgacggct
     3841 gcctgtgaag aagtttcgcg aagttcgaaa tgaaggtgag gc
//
LOCUS       DAORPD010000045         3849 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_4124_length_3849_cov_1.940169, whole genome shotgun sequence.
ACCESSION   DAORPD010000045 DAORPD010000000
VERSION     DAORPD010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4124_length_3849_cov_1.940169"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="PK976_00955"
     CDS             <1..450
                     /locus_tag="PK976_00955"
                     /inference="COORDINATES: protein motif:HMM:NF014655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HRC66489.1"
                     /translation="VLFFFIRNNERTEISLFIIAGLGHESIVKSSEHKTSFIENVRDI
                     EFFRTFMAGKIWEDYMEETKEKTIAQEVTGVIGVKDLILFNDDVNTFEYVIECLMEVC
                     QHTFEQAEQCATIAHLKGKCPIKMGCYEELKPICDELIRRKLTVAIK"
     gene            455..919
                     /locus_tag="PK976_00960"
     CDS             455..919
                     /locus_tag="PK976_00960"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HRC66490.1"
                     /translation="MSWSIIIILIVVGLIFMVLEILVIPGTSVGGIIALACVGIAIWQ
                     SFAAHGMIAGSITLGITLAVSFIVLYFSLKSNTWKRLMLTQQNTAKVNVINEDIVKPG
                     IQGLTLSRLAPAGKIEIEGQEYEAHTFGEYVDPGQKVIVVKIEYNKIYVKPI"
     gene            930..1934
                     /gene="floA"
                     /locus_tag="PK976_00965"
     CDS             930..1934
                     /gene="floA"
                     /locus_tag="PK976_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861713.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HRC66491.1"
                     /translation="MSSTLLIIIIGLVALFGLWIIFYFIPVGLWFSALVSGVRISLIQ
                     LILMRWRKVPPSVIVNNLIAATKAGLKINANDLETHYLAGGRVKSVVNALISAEKANM
                     GLDIKTATAIDLAGRDVFEAVQMSVQPKVIDTPPVTAVAKDGIQLIAKARVTVRANIR
                     RLVGGAGEETILARVGEGIVSSIGSAESHKIVLENPDSISKLVLQKGLDSGTAFEILS
                     IDIADIDVGKNIGAVLQMDQANADKNIAQAKAEERRAMAVALEQEMKAKAQEARAKVI
                     EAESEIPKAIAEAFRNGNLGIMDYYRFENLKADTKMRESIATPPAKPKNTKDDDLTQQ
                     "
     gene            complement(2120..>3849)
                     /locus_tag="PK976_00970"
     CDS             complement(2120..>3849)
                     /locus_tag="PK976_00970"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HRC66492.1"
                     /translation="EFNPIYSWTEWNHAYIDMQKIYLVPLSKLTTSPFAPTTELNASA
                     IEKKPEPQAKKDETKSPKASPEVKIDFDGIETRSIAIEVDPGRFWNLAPTEKGIYYNR
                     TNSKESKALLCYFDLNNKKETVIGEYHAFVLAPDGKKMMIAARGKYYIIDAPQSKIQP
                     EESLNLSDMKVFVNLKQEWAEAFKESWRQMRDFFYDLNMHGVNWKAIYQKYEPMVKYV
                     NNRNDLNYIIGEMVGELNIGHAYVGGGDKPEPQRIKTGLLGARLSKDASGYFKIDKIL
                     AGENWDPSLRSPLTEPGLNIREGDFILAVNGQPTNQMLDIYISLVGKAGSLVELSIAS
                     TPDPSASRKVIVKPIESEAGLYYFNWVRSNIEKVNKATNGQVGYIHIPDMGVEGLNEF
                     AKYFYPQLDKRALIIDDRGNGGGNVSPMIIERLRREVATFSVQRNGAVTTRPSQMIWG
                     PKVLLIDRYSASDGDLFPYQFKYYKLGKTVGQRTWGGVVGIRGTLPIIDGGMLNKPEF
                     APYDVEGKSFIIEGYGVEPDIEIWNDPAKEYEGVDEQLNKAIEVILEELQNWPEQFPP
                     VPPYPDKSK"
BASE COUNT         1129 a          803 c          821 g         1096 t
ORIGIN      
        1 gtcctattct tcttcatcag gaataatgag agaacagaaa tttctctctt cattattgcc
       61 ggactcggac atgaatcgat agtgaagtca tccgaacata aaacttcatt tatcgaaaat
      121 gttcgagaca ttgaattttt ccgtactttt atggccggta agatttggga ggattatatg
      181 gaagaaacca aggaaaaaac tattgctcaa gaggtaacag gggtaatagg ggtcaaggat
      241 ctgatcctat tcaacgatga tgtgaacacc tttgaatatg tgatagaatg tttgatggaa
      301 gtttgccagc acaccttcga acaagcggaa caatgcgcta ctattgcaca cttgaagggg
      361 aaatgcccca taaaaatggg atgttatgag gaacttaagc ctatatgcga tgaattgatc
      421 cgacgtaaat taaccgtcgc aatcaaataa ccccatgagt tggagtatta tcatcatttt
      481 aattgtagta gggcttatat ttatggtcct cgaaattctg gtgattcccg gaacttcggt
      541 aggaggtatt attgcactgg catgtgtagg cattgctata tggcagagtt ttgctgccca
      601 tggaatgata gccggttcga tcactttagg tattacattg gctgtcagtt tcatagtgct
      661 ctatttttct ttgaagtcga atacatggaa gcggctcatg ctaacccagc aaaatacggc
      721 caaggtgaat gtcatcaatg aagacatcgt aaaacccggt atacaggggt taaccctttc
      781 acgactggca cctgctggaa agatcgaaat tgaaggtcag gaatatgaag ctcatacatt
      841 cggagaatat gtcgatcctg gccaaaaggt gatagtcgta aagatagaat acaataaaat
      901 ttatgtaaaa ccaatttaat caacatttta tgtctagtac actattaatt attattatag
      961 ggctggtagc tctattcggt ttatggatta ttttttactt tattcctgta ggtttatggt
     1021 tttcagctct ggtgagcgga gttagaatct ctttgataca gcttattttg atgcgatgga
     1081 gaaaagtacc tccctcggtc attgttaata atttgattgc tgccacaaaa gctggattga
     1141 agatcaatgc taatgatctt gaaacacatt atttagctgg aggacgcgta aaatcagtgg
     1201 tcaatgcttt gatctctgcc gaaaaagcca acatgggttt ggacatcaag acggccacag
     1261 ccatcgattt ggctggaaga gacgtatttg aagccgtaca aatgtcggtt cagcccaagg
     1321 taatcgatac tcctccagtt acggccgttg cgaaagatgg gattcaactt atagccaaag
     1381 ccagagtaac cgtgagagca aacatcaggc gactggttgg gggtgcaggc gaagaaacta
     1441 tactagcccg agtaggagaa ggcatagttt cgagcattgg ctcggccgaa agccataaaa
     1501 tcgtgctcga aaacccggat tccatctcta aactggtgct gcaaaaagga ctggactcag
     1561 gcacggcctt cgaaatcctt tctatcgata ttgccgatat cgatgtaggg aagaatattg
     1621 gagccgttct tcagatggat caggccaatg ccgataaaaa catcgctcaa gccaaggccg
     1681 aagaaagaag ggctatggca gtagcactcg aacaggaaat gaaggcaaag gcacaagaag
     1741 cccgcgctaa ggtcatcgaa gcagaatcgg aaatccctaa agccattgcc gaggctttcc
     1801 gcaacggcaa cttgggcatc atggattatt accgtttcga aaacctgaaa gcagatacga
     1861 aaatgcgcga aagcattgcc accccacccg ctaaacctaa aaataccaaa gatgacgact
     1921 tgacacaaca gtaatgagag atcggtggat ggtgattagt ggatggcggt tagagtttgt
     1981 gagttgtaag ttgtgtgtta taagttgtta attggttatc aatttgtcaa taggcgtttt
     2041 acgtattcca ttagcttatt aaaaacgcac tgcgcgttat caatttacaa cacgcagtgc
     2101 gtttcattta tttatttatt tatttcgatt tatctggata aggtggaaca ggtgggaact
     2161 gttcgggcca gttttgcaat tcctcgagta ttacctcgat agccttattt aattgctcat
     2221 ctactccctc gtattcttta gccggatcgt tccatatttc tatgtcgggt tcaacaccat
     2281 aaccttcaat aatgaaggat tttccttcca catcataagg agcaaattcg ggcttattga
     2341 gcatgccacc atctataatg ggcaaagtac cacgaattcc gactacacct ccccatgtac
     2401 gctgtccaac ggttttcccc aatttataat atttaaactg ataagggaaa aggtcgccat
     2461 ccgaggctga atagcggtca ataagcaaaa cctttggacc ccaaatcatt tgtgaaggac
     2521 gtgtagtaac cgctccattc ctttgaacac tgaatgtagc cacttctcgc ctcaatcttt
     2581 cgataatcat aggagaaaca tttccacctc cattgccacg gtcgtctata atcaaggcac
     2641 gtttatccag ttgggggtag aaatactttg caaactcgtt caatccttca actcccatat
     2701 cgggaatatg gatataccca acttgcccat tggtagcttt gttaaccttt tcgatatttg
     2761 atctaaccca attgaaataa tacaaacccg cttcgctttc aatgggtttt acgatcactt
     2821 ttcttgatgc tgaaggatca ggggtggaag caatgctcaa ttctaccaaa gagcctgcct
     2881 tacccaccaa agaaatataa atatcgagca tttgattggt aggctggcca tttactgcaa
     2941 gaataaaatc gccttccctg atattcaaac ccggctcggt gagaggtgag cgcaaagaag
     3001 gatcccaatt ttctccagcc agtatcttat caattttaaa ataacctgaa gcatccttac
     3061 tcaaacgcgc ccccagaagc ccagttttta ttctttgagg ttcgggtttg tcgccacccc
     3121 ccacgtaagc atgtccaata ttcaactcac caaccatttc accgataata taattcaaat
     3181 cattgcggtt gttgacatac tttaccatgg gttcatattt ctgatagatg gctttccaat
     3241 ttacgccatg catgttgaga tcatagaaaa agtcgcgcat ctgtcgccac gactccttaa
     3301 aagcctcggc ccactcctgc ttcaaattca caaaaacctt catatctgat aaattgagcg
     3361 attcttcggg ttgaatcttt gattgggggg cgtcaataat ataatatttc cctcgagcag
     3421 caatcatcat cttcttacca tctggagcaa gaacaaaggc atggtactct ccaataacgg
     3481 tttccttctt attattcaag tcgaaataac aaagcaaggc tttcgattcc ttagaattag
     3541 tacgattgta atatatccct ttttcggtag gtgcaagatt ccagaaacga ccgggatcga
     3601 cttcaatggc aatagaacgg gtttcgatcc catcgaaatc gatcttaacc tcaggggagg
     3661 ccttaggcga tttagtttcg tccttttttg cctgaggctc tggtttcttt tctatggcag
     3721 aagcattcaa ttccgtggtt ggggcaaagg gagaagttgt aagttttgat aaaggaacca
     3781 gataaatttt ctgcatgtcg atgtaagcat gattccattc tgtccatgaa taaatgggat
     3841 tgaactcac
//
LOCUS       DAORPD010000046         3837 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_4150_length_3837_cov_2.510841, whole genome shotgun sequence.
ACCESSION   DAORPD010000046 DAORPD010000000
VERSION     DAORPD010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3837
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4150_length_3837_cov_2.510841"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1941
                     /locus_tag="PK976_00975"
     CDS             <1..1941
                     /locus_tag="PK976_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66493.1"
                     /translation="TYFWQLMRGLTNFHIRELDKSNPVLVSRDGVFQDTIYYYRKYDA
                     NSQRTFDRNLRKKLGLPVDGLDFINIDSYDFASKTINYYDKDGNLKTVTMGEDLFDVS
                     LFDADELLNGGNSYVAYYGYDYTGKKLSSQPSFEDFLTKKNADGEFLREVPAFRPNYL
                     ATYIQDKFAFKDLIFNVGLRVDRYDANQLVLKDPYSIYPTLSVKEVQKIGDQIVSHPG
                     NMGSDYVVYVDNATNPTTITGYRNGSVWYNATGIEIADPYILDAGSGVTPYLRNPSNQ
                     TLQKEAFQDYDPQWAFMPRISFSFPISDEALFFAHYDILTQRPQGENRFDITDYYFFK
                     TRGTTLDNPNLKSPKTIDYELGFKQKLTNASALTLSAYYKEIRNEIQIYRYAGAYPRD
                     YISYNNIDFGTVKGFTISYDLRRTSNVTMRASYTLQFADGTGSSPTTAAALIAARLPN
                     LRTPMPLAWDRRHSFNVNIDYRYGEGKEYNGPRISRKNKPPVELLRNTGVNFTVIGGS
                     GTPYTRSENITSAVSGGTRILKGSFYGSRLPWQFRIDAKLDKDIHIVWKKKEGEKPKE
                     SMLNIYLQMLNIFNFKNVMAVYPATGNPDDDGYLAAAEWQKEINQQLNPDTYRQLYRI
                     YINNPSNYSSPRMIRLGLIFNF"
     gene            2002..>3837
                     /locus_tag="PK976_00980"
     CDS             2002..>3837
                     /locus_tag="PK976_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66494.1"
                     /translation="MMKMYQRILLIATIASLISINIYGEEFVGDKLKQSKNVKASTAG
                     CTAASAYQFLDINNVRCRINTGGDMWWNLDTGAGSRGQYFIPANTTKSSLFVGSLWIG
                     GLDVNDQLKLAALRYRQIGNDYWTGPLTIDGTAAVDAAVCAEYDKFFVISRAEVDEFL
                     GWWYSTNRSEEYPGYSIPKSIMDWPAHGDPSKFQSYYLAPFYDMDGNGEYDPNQGDYP
                     YYDIPNELCHSPVQTMEGINGIVKGSILADQVLKGDKTLWWVFNDKGNIHTESSGQAI
                     GLEIRAQAFAFSTNDEINNMTFCSYEIINRSTFTLTQTYFAPNTDPDLGYAWDDYVGC
                     DVLRGMGYCYNGKAVDGTGQSEAYGDQPPAVGIDFFQGPYLDPDGYDNPSFLADGILG
                     PSFNGDCSIVNFDSTVIVMTYGKPGEEKTGNFMVRSAAINGVNFGNGIVDDERFGMRR
                     FVYYNNAGGIQGDPSIAAEYYNYLRGIWKDNTKMVYGGNAHPSNPGAVGPETDFMFPG
                     DSDPCNWSTGGAPPNGGLNQNGLYWTEVLAGNAPSDRRFVQSAGPFTLEPGAVNYITF
                     GVPWARAISGGPYASVELLRVVDDKCQALFDNCFKVLDGPDAPDLT"
BASE COUNT         1087 a          845 c          824 g         1081 t
ORIGIN      
        1 acctatttct ggcaattaat gagaggactt actaacttcc atattcgtga attggataaa
       61 tctaacccgg ttcttgtttc ccgcgatggg gtctttcagg atactatcta ttattacaga
      121 aaatatgacg caaattcaca acggactttc gaccgcaatc tgcgtaaaaa attaggtctt
      181 ccagtagatg gccttgattt tatcaatatt gattcgtatg attttgcttc gaaaaccatc
      241 aattattacg ataaggatgg aaacttgaaa accgttacca tgggtgagga tctattcgat
      301 gttagtctct tcgacgccga tgagctatta aatggaggca attcgtatgt agcttactat
      361 ggatatgatt atacgggtaa gaaactctcc agtcaaccat cattcgaaga tttccttacg
      421 aagaagaatg ccgatgggga gttcctacgt gaagttcctg cttttcgtcc caattacttg
      481 gctacctaca ttcaggataa attcgctttt aaggacctga tattcaatgt gggcttacgt
      541 gtggaccgct acgatgccaa tcagctcgtt ttaaaagacc cttactcgat atatccaacc
      601 ctttcggtga aagaggttca aaaaatagga gaccaaatag ttagtcatcc aggaaatatg
      661 ggctctgatt atgtggttta tgtggataat gccaccaatc ccaccaccat tacaggctat
      721 cgtaatggca gtgtatggta taatgccact ggaatcgaaa tagccgaccc ttacattctg
      781 gatgccggtt ctggagttac tccctactta cgaaatccgt ccaatcaaac cttacaaaag
      841 gaggcattcc aagattatga cccgcaatgg gccttcatgc ctcgaatctc tttctcattc
      901 cctatttccg acgaggcttt gttctttgcc cattatgaca tccttaccca gcgtcctcag
      961 ggagaaaacc gattcgatat caccgattat tacttcttta aaacccgtgg aactaccctc
     1021 gataatccga atctgaaatc tcccaaaacc attgactatg aattaggttt taagcaaaaa
     1081 ttgacaaatg cttcagccct taccctatcg gcatattata aagaaatccg gaatgaaata
     1141 cagatttatc gttacgcggg agcttatccc agagattata tatcctataa caatattgac
     1201 tttggtactg taaaaggatt taccattagt tacgatttga gacgtacgag caatgtaacc
     1261 atgcgtgctt cctatacctt gcagtttgcc gatggtacgg gttcgagccc aaccactgcc
     1321 gctgcactca tagctgcacg cctgccaaac cttcgtacac ctatgcccct tgcatgggat
     1381 agacgccata gcttcaatgt caatatcgat tatcgctatg gagaagggaa agaatacaat
     1441 gggcctcgta tcagccggaa aaacaagcct cctgtcgaac tcctcagaaa tacaggcgtt
     1501 aatttcacgg taattggtgg ttcgggaacg ccttacaccc gttcggaaaa cattacttcg
     1561 gcagtaagcg gtggtacacg tatcttaaaa ggaagcttct atggctcaag actgccatgg
     1621 cagttccgaa tcgacgctaa gctcgataaa gatatccaca tagtctggaa aaagaaagaa
     1681 ggagaaaagc ctaaggaatc catgctgaat atttatttgc aaatgctcaa tattttcaat
     1741 ttcaagaatg tgatggccgt ttatccagct acaggcaacc ccgacgatga tggttatctt
     1801 gctgccgccg aatggcagaa ggaaatcaat cagcagctca atcccgatac ctatcgccag
     1861 ctctatcgta tctatatcaa taatccatct aattacagca gtccacgaat gattcgcctg
     1921 ggcttgatat tcaattttta atcttacatt attgaaagat gatgtataag ctttgatatg
     1981 ataataaaca aaacgaataa tatgatgaaa atgtatcaac gcattctact aatcgccacg
     2041 atagcttcgc ttattagtat aaatatctac ggtgaagaat ttgtgggcga caagttaaaa
     2101 caatcgaaaa atgtaaaggc aagcacagca ggatgtactg cagcttctgc ttaccaattc
     2161 ctcgatatca ataatgtgcg ctgccgcatt aatacaggag gagatatgtg gtggaatctc
     2221 gatacaggtg ctggaagccg tggtcaatat tttatacctg ccaataccac taagtcttct
     2281 ctttttgtcg gctctttgtg gattggtggc ctcgacgtca acgatcagtt aaagcttgct
     2341 gctttgcgtt atcgacaaat tgggaacgat tattggactg gtcctcttac cattgatggc
     2401 acagcggctg tggatgctgc cgtctgtgct gaatatgaca agtttttcgt catcagccgt
     2461 gccgaagtag atgaattcct cggatggtgg tacagtacga atcgttccga agaatatccc
     2521 ggttacagca ttcccaaatc catcatggat tggccagctc atggcgaccc atcgaagttt
     2581 cagagttatt acctcgctcc attttacgat atggatggga atggagagta cgaccccaac
     2641 caaggagact atccctatta tgatattccc aatgaacttt gccatagccc cgtacaaacc
     2701 atggaaggga tcaatggcat cgtaaaaggg agtatcttgg ccgaccaggt tttaaaagga
     2761 gataaaaccc tatggtgggt atttaatgat aaaggaaata tacataccga atcgagcgga
     2821 caggctattg gtcttgaaat tcgtgcacag gcttttgctt tctccacaaa cgacgaaatc
     2881 aacaacatga ctttctgcag ttatgaaatt atcaaccgtt ccacttttac cttaacccaa
     2941 acctatttcg ctcctaatac tgaccctgat ttaggttatg catgggatga ttatgtagga
     3001 tgcgatgtat tgagaggcat gggatattgc tataatggaa aggccgttga cggaacaggt
     3061 caatcagaag cctatggcga tcaacctcct gccgtgggga tagacttttt ccaagggcct
     3121 taccttgacc ctgatgggta tgataatcct tctttcttgg cggatggcat tcttggtcct
     3181 tcatttaacg gagattgctc catcgtgaac ttcgattcta cggttattgt aatgacttat
     3241 ggtaaacccg gtgaagaaaa aacggggaat ttcatggtga gaagtgcagc catcaatgga
     3301 gtgaattttg ggaatggaat cgttgacgac gaacgctttg gaatgcgcag gttcgtttat
     3361 tataataacg ccggtggtat tcagggcgac ccaagtatag ccgctgaata ttataattac
     3421 ctacgtggaa tttggaaaga caatacaaaa atggtttatg gcggaaatgc acaccccagc
     3481 aatcccggag ccgttggccc cgaaaccgat ttcatgtttc ccggtgattc cgatccctgt
     3541 aactggtcaa ctggtggtgc acctcctaac ggcggtctta accagaatgg gctatactgg
     3601 acagaagttc ttgctggaaa tgcaccttcc gaccgtcgtt ttgttcaatc agcaggtcca
     3661 tttactcttg aacccggagc cgtgaattat atcacttttg gtgtaccatg ggctagagcc
     3721 atttccggag gaccttatgc ctccgttgaa ctcctgcggg tagttgatga caagtgtcag
     3781 gctcttttcg acaattgctt taaagttctc gatggtcccg atgctcccga tttgact
//
LOCUS       DAORPD010000047         3755 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_4279_length_3755_cov_2.058378, whole genome shotgun sequence.
ACCESSION   DAORPD010000047 DAORPD010000000
VERSION     DAORPD010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3755
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4279_length_3755_cov_2.058378"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..949)
                     /locus_tag="PK976_00985"
     CDS             complement(<1..949)
                     /locus_tag="PK976_00985"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HRC66495.1"
                     /translation="MDHTISSLATIVTPIWVSLTKPDDMIRFLLTDSRRLVEAKSTLF
                     FAITTSRNDGHHYVEDLFKKGVRNFVVTNTQLFPNLLGHANFIQVENTINALQKIAAA
                     HRAEFQIPILGITGSNGKTIVKEWLTQMILTKLCVCASPDSYNSQIGVPLSVWQLNDY
                     HQLGLFEAGISQPHEMESLQKIIKPNYGIFTNIGQAHSENFGTLEEKTIEKTKLFRET
                     EVLIYCRDHELIHKVVKENLKSKTKLITWGENPESTIRIIQKTRNGKTTSLTLVNNQQ
                     KIQLELPVSDMASIENAMHCYTFLDCLGWDDKTKAEAILQ"
     gene            complement(964..2088)
                     /gene="pdxA"
                     /locus_tag="PK976_00990"
     CDS             complement(964..2088)
                     /gene="pdxA"
                     /locus_tag="PK976_00990"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005847333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HRC66496.1"
                     /translation="MDNDILNLDPYENSDLQEPEMIRIGFTQGDINGIGYEIIMKALS
                     EIGTPYSFIPILYGSSKIASYHRKTLSIPDLPFNHVRKASQALPHKVNIVNITDKEIR
                     VNLGQSTPEAGEMAYLSLHQACRDLEDGLLNVLVTAPINKYNIQSPEFHFPGHTEFLA
                     NRFRAANPIMTMVYNNLRIGFVTNHVPVRQIADNITTDLVYNKIKALNLSLFQDFGIS
                     HPKIAVLSLNPHAGDRGLLGSEENEIISPAIHQAFEEGILALGPFPADGFFGMDHYRK
                     FEGILAMYHDQALPVFKILAQDYGVNFTAGLPFVRTSPAHGTAYDIAGKDKASHHSMR
                     EAIYLAIDVFKNRLEYARITANPLRSPIQQNSEETEEEEI"
     gene            complement(2090..3550)
                     /locus_tag="PK976_00995"
     CDS             complement(2090..3550)
                     /locus_tag="PK976_00995"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HRC66497.1"
                     /translation="MNILLKQQYTSEDFNTHCAFLFPSLEDIPEKWLSTQELNFVKRV
                     NTNSPDETVIPVHRANYSWFCILPKPNENPAKKLENIRLAGYQLTQFLHKERIDELHI
                     SSFHASSLDLFALAEGIVLSSYAFQKYQNNEKKRKHQLQTLDILSENFDYKQLKELEI
                     ITEAVYLCRDLVNEIPSELNAKSLAEILKQKAEEVGIRAEVMNKKKIESLKMGGLLGV
                     NKGSTIPPTFTILEWKPFNFINEKPIILIGKGITFDSGGMNIKTGTYMENMKSDMAGA
                     ATVASVVIAAARLSLPLNVMAFVPATDNRINSDAMVSGDILTMYNGKTVEVVNTDAEG
                     RLILADALSYANKFDPFLTITAATLTGAAARAIGKYGIVAMESHARTQFDLLKQSGEE
                     VYERLVEFPLWEEYFDLIKSDVADIKNSGPAEAGAITAGKFLEYFTTYPFIHLDIAGT
                     SFTNKNDGYRGIGATGIGVRLLISFLQNVIQNHQNS"
BASE COUNT          987 a          713 c          843 g         1212 t
ORIGIN      
        1 gctgaagtat ggcctcggcc tttgttttat cgtcccatcc taaacaatct aagaaggtat
       61 aacagtgcat ggcattctct attgaagcca tatcgcttac cggtaattcc aactgtattt
      121 tctgttgatt attgaccaat gtaagggaag tagttttgcc gtttcttgtt ttttggatga
      181 ttcgaattgt cgattccgga ttttctcccc aggtaatgag ctttgtcttt gatttcagat
      241 tctccttcac taccttatga atcagttcat gatctctgca atagatcagc acttccgttt
      301 cccggaagag ttttgttttt tctatggttt tttcttcgag ggtgccgaag ttttcggaat
      361 gagcttgtcc aatattggta aaaattccat agttgggttt aataattttt tgaagtgatt
      421 ccatctcatg gggttgagaa atccctgcct cgaataatcc cagttgatgg taatcgttta
      481 attgccaaac cgaaagaggt acaccaattt gagagttgta gctatcggga ctagcgcaaa
      541 cgcaaagttt agtcaaaatc atctgggtga gccattcttt cactattgtt tttccgttac
      601 ttcctgtgat tccaagtata gggatctgaa attcggcccg gtgagcagca gcaatctttt
      661 gtaatgcatt tatggtattc tctacctgta taaaattggc atgtcctaat aaatttggga
      721 agagttgagt attggtgacg acgaaatttc ttactccctt tttgaaaaga tcttctacat
      781 agtggtggcc atcgtttcgt gatgtagtaa tagcaaaaaa aagtgtagat tttgcctcta
      841 ccaatcgtcg gctatctgtc agcagaaatc gtatcatatc atcaggcttt gtcaaggaaa
      901 cccagatagg agtaactatg gttgcaagtg atgaaatggt atgatccatt gctgacctgt
      961 gagttatatt tcttcctctt cggtttcttc ggaattttgc tgaataggtg aacgaagtgg
     1021 attggcagtt atccgggcat attcgaggcg gttcttgaaa acatcaatag ccagataaat
     1081 cgcttctctc atggaatgat gcgaggcttt gtctttccct gcaatgtcgt aagctgtgcc
     1141 atgggcgggt gatgttctta cgaaaggaag tccagccgta aaatttactc catagtcttg
     1201 ggctagaatt ttgaatacgg gtaatgcttg atcatgatac atggccaata ttccctcgaa
     1261 tttcctatag tggtccatcc caaaaaatcc atccgcagga aaaggtccaa gagcaagaat
     1321 accttcttcg aatgcttggt gaatggcggg gctaatgatt tcattttctt cgctgccgag
     1381 taatccgcga tcaccagcat ggggattaag gcttaataca gcaattttag gatggcttat
     1441 gccaaaatcc tgaaataagg ataaattcaa tgcctttatt ttattgtaaa ccaggtcggt
     1501 agtgatatta tcggcaattt gacgcacagg aacgtgattg gtaacgaacc ctatccttag
     1561 attattgtat accatggtca tgatgggatt ggcagcacga aagcggttgg ccagaaactc
     1621 cgtatgtccc gggaaatgaa actctggaga ttgaatattg tacttattaa tcggagcagt
     1681 aaccaaaaca ttcaatagcc catcttccaa gtcacggcag gcctgatgga gtgagagata
     1741 ggccatttca cctgcttcag gtgttgattg tcctaaattt accctgattt ccttatcagt
     1801 aatattgacg atatttactt tgtgaggaag ggcttgagaa gccttacgta catgattgaa
     1861 tggcagatcg ggtatgctga gggtttttcg gtgataggaa gctattttgg atgagccata
     1921 cagaatagga ataaaactat agggcgttcc aatctcggaa agagctttca taattatttc
     1981 atagccaatt ccattgatat cgccttgggt aaatccaatc cgaatcattt ccggctcctg
     2041 aagatcggaa ttttcataag ggtcgaggtt caagatatcg ttatccatat taagaatttt
     2101 ggtggttttg aataacattt tgcaggaagg atataagcag tctcacaccg attccggtgg
     2161 ctccaattcc cctatatcca tcgtttttat tggtgaacga agttcctgca atatcgaggt
     2221 gaatgaaagg gtaagtggta aaatattcga ggaacttgcc tgcggtgatg gctccagctt
     2281 cggctggccc tgaatttttt atatcggcaa cgtcggattt tatcaagtcg aaatattctt
     2341 cccatagggg aaattctacc agccgttcgt atacctcttc tccactttgc ttgagcaagt
     2401 cgaactgggt tctggcatgt gattccattg ccactattcc atatttacca atggctctgg
     2461 cggctgcacc tgtgagtgtg gccgcagtaa tcgtaagaaa aggatcgaat ttattggcat
     2521 agcttaaggc atcggccaga atcaatcttc cttcggcgtc ggtgttcacc acctctactg
     2581 tttttccgtt atacatcgta agaatatctc ccgagaccat ggcgtcggaa ttgatgcgat
     2641 tgtcggtggc tgggacgaat gccatgacat tcaggggcaa tgatagtctg gctgcggcaa
     2701 ttacaacgga agctacagtg gcggcacctg ccatgtcgga tttcatattc tccatataag
     2761 tgccagtctt gatgttcata cctccactat cgaaagtgat tcccttgccg attagaatga
     2821 tgggtttttc gtttataaag ttaaaaggtt tccattcgag tatggtgaat gtgggcggta
     2881 tcgtacttcc tttgtttact cccagtaagc ctcccatttt caaagattct atctttttct
     2941 tgttcataac ctcggctctt atgccaacct cttcggcttt ttgttttaga atttcagcca
     3001 gacttttggc gttcaattca ctgggaatct cattcaccaa atcgcgacat aagtaaacag
     3061 cttcagtaat gatttcgagt tctttcagtt gtttgtaatc gaaattttct gataaaatat
     3121 cgagggtttg caattggtgc tttctctttt tttcgttatt ttgatatttc tgaaaagcat
     3181 agctacttaa tacgatgcct tcggccaatg caaatagatc aagtgaagaa gcatgaaaag
     3241 aagaaatatg cagttcgtcg attctttcct tatgtaaaaa ttgagtgagc tgataacctg
     3301 ctagtcggat gttttcgagt tttttcgctg ggttttcatt tggcttaggc aagatacaga
     3361 accatgaata attggctcgg tgaacaggga taaccgtttc atcgggtgag tttgtattta
     3421 ccctttttac aaagttcaat tcttgcgtac tcagccattt ttcgggaata tcttcgagtg
     3481 atgggaaaag aaatgcacaa tgagtattga aatcctcaga ggtatattgt tgttttagca
     3541 ggatattcat tgggtttatt gcttctagtg cgcaaagtta aaggaattcg gataaacttt
     3601 cataatttct ttttccgatc cctggcagag gctgaatatg aatgagtggg tggttgattt
     3661 cagtggaagg ataaatacag ttattttaat gaattaacgg aggggaggtg ttctggggaa
     3721 aatgtgggaa gccgccccga aaaatttcga ggcgg
//
LOCUS       DAORPD010000048         3668 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_4417_length_3668_cov_2.487130, whole genome shotgun sequence.
ACCESSION   DAORPD010000048 DAORPD010000000
VERSION     DAORPD010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3668
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4417_length_3668_cov_2.487130"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..814)
                     /locus_tag="PK976_01000"
     CDS             complement(176..814)
                     /locus_tag="PK976_01000"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRC66498.1"
                     /translation="MQRGLIVFSMKDLERMKERVAHLPMKIEENEISVLPVAEYQGEI
                     VVIERLRNLSQALEEIYSWGYVGFDTETRPNFHKGENHKPALMQVAGARKTFLFRLNK
                     LGFPPLLRSFISDENIIKVGLALENDLPELTLHQKFSPAGMVDLNQLCPQLGFQNIGA
                     RKLTAIFLKVRLKKSQQTSNWENSILTEAQLKYAALDSWICREIYSILMGIV"
     gene            complement(825..3389)
                     /locus_tag="PK976_01005"
     CDS             complement(825..3389)
                     /locus_tag="PK976_01005"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRC66499.1"
                     /translation="MKTKPLFIADFRIFILFISIVLTSYTLFSQKKPMAQVSGYISNQ
                     HGEPLELVNISIEGLPGGTSTDRNGFFQLRVPAEIEVNLIVSFIGFKTERIPLHLHQG
                     EKITIRRTLEIQNTLLSPVSIEDHQIRSSSLTRIDPKAATTIPTLGGGIEALIKTMPG
                     VTSSNELSSQYSVRGGNYDENLVYVNDVEIYRPFLIRSGQQEGLSFLNPDLVSSILFS
                     AGGFEAKYGDKMSSVLDIQYKKPAQFGGSASLSLLGGSFQIEGISKNKKLNYLLGIRQ
                     KTNQYILSSQQTKGDYKPSFTDVQANVNYNLHPRLEISALGNFSINLFRLVPHDRETS
                     FGTIQEAYRLRVFFEGQEDDKFVNGMGAITATWKPCDPLRLKFITSAFRTVETETYDI
                     LAQYWIGDLETDQTNGGSGKVVEERGVGTYLNHARNRLEATVASFEHKGTFLLPNNNY
                     MNWGVKYQHESIKDKLNEWTLIDSAGFALPLTIDSVGYTHPQIQPPLQIPMNEYLYSK
                     NTLNSNRLSAYFQRSWNFISDSTKFSLTAGIRSQYWDLNHQWLFSPRATLSITPNWKK
                     DIIFRISTGYFYQPPFYRELRDYQGIIHTDVKAQKSVQVVAGADLNFKAWSRNFKFVA
                     EAYYKYFDHLIPYDIDNVRIRYYAKNNAHGYAAGIDLKVNGEFVKGIESWASLSLMKT
                     MEDIEDDYYWEYYNQAGEKIIPGFTTDTRAVDSIKIEPGYLPRPSDQRVSFNLFFQDY
                     LPNNPTYKMHLNLVFGTGLPFGAPKSPRYQHIYRTPSYRRVDIGFSKLLKSEASNAPG
                     FLQYFKSIWITAEVFNLLQFNNTVSYSWISDVNGRRYAIPNYLTPRLVNLRLIFNF"
BASE COUNT         1042 a          706 c          786 g         1134 t
ORIGIN      
        1 tacctaccca atcaatgaaa gatgaatgtc agaccctact cattgaatct tgtctttaga
       61 agagattcag cggttgcttt tggtttcatc gataagcttc aacatctatg catcaaaaat
      121 gatggcgtat agatttggac agttgaaaaa atgaaggaat cgttaatctc tgggtttata
      181 ctatacccat gaggatagaa tatatctcac gacagatcca agaatcgagt gctgcgtatt
      241 tcaattgtgc ttcggtaagg attgaatttt cccagttgct tgtttgctga ctttttttga
      301 gccttacttt taggaaaata gcggttaatt ttctcgctcc aatattttga aaaccaagtt
      361 gtggacataa ttgattgagg tcgaccatgc cggcaggcga aaatttctgg tggagtgtaa
      421 gttcgggaag atcattttcg agggctaagc ctacttttat gatgttttcg tcgcttataa
      481 acgagcgcaa taaaggtgga aaccccagtt tattgagccg aaacagaaag gtttttcgag
      541 cgccggcaac ttgcataagg gccggtttat ggttttctcc cttatgaaaa ttcggacgtg
      601 tttcggtatc gaaacctaca taaccccatg aatagatttc ttccaaagcc tgacttagat
      661 ttcttaatcg ttctatgact acgatttctc cttgatattc agccacaggt aagaccgata
      721 tttcattttc ttctattttc atcggaagat gagccactct ttctttcatc ctttccagat
      781 ccttcattga aaatactatc aggcctcttt gcatttccct tggattaaaa attgaatatc
      841 aagcgcagat tgaccaaacg tggtgtaagg taattaggta tggcatatct tctgccattg
      901 acatcggaaa tccatgaata ggaaactgtg ttattgaatt gcaaaagatt aaacacttcg
      961 gcggtaatcc aaatagattt gaaatactga agaaatcctg gtgcattgga agcttcgctt
     1021 ttgaggagtt tcgaaaaacc aatatcgact cttctatagg aaggagtgcg gtaaatatgc
     1081 tgataacggg gcgattttgg agctccaaag ggaagtcctg tgccaaaaac cagattcaaa
     1141 tgcatcttgt aggtgggatt attgggaagg taatcctgaa aaaacagatt aaagcttact
     1201 ctttgatcgg aaggacgagg taaataacct ggctcaattt ttatactatc aacggctctg
     1261 gtatcggtag taaatcctgg aataattttt tcacctgcct gattataata ttcccaataa
     1321 tagtcgtctt caatatcttc catcgttttc atcagagaaa ggcttgccca tgattctatt
     1381 ccctttacga attcaccatt taccttcaaa tcgattcctg ctgcgtatcc atgcgcattg
     1441 ttcttcgcat agtagcggat tcttacattg tcgatgtcgt agggaataag atgatcgaaa
     1501 tacttgtaat aagcttcggc aacgaattta aaatttcgac tccatgcttt aaagttcaaa
     1561 tcggcacctg ccacaacctg aacagatttc tgagccttta catctgtatg aatgattccc
     1621 tgataatcgc gcagttccct ataaaatgga ggttgataaa aatatcccgt cgaaattcga
     1681 aagatgatgt cttttttcca gttgggtgtt atggaaagcg tggctcttgg gctaaataac
     1741 cattgatgat tgaggtccca atattgagac cttattcctg cagtaagtga aaacttcgta
     1801 ctgtcggaaa tgaagttcca tgaacgctga aaataagcag ataaacggtt agaattcagt
     1861 gtattttttg aataaagata ttcgttcatg ggaatttgga ggggaggttg aatttgaggg
     1921 tgggtataac ctaccgaatc gatggtaagt gggagggcaa agcctgccga atcgatcaaa
     1981 gtccattcgt tgagtttatc tttgatgctt tcgtgttgat attttacacc ccagttcatg
     2041 taattattat tgggcagcag aaaagttccc ttatgctcaa atgaggctac ggtggcttcg
     2101 aggcggttgc gagcgtgatt gaggtaagta cctactccac gttcttcgac cactttgcct
     2161 gagcctccat tggtttggtc tgtttccaaa tcacctatcc agtattgtgc taaaatgtcg
     2221 taagtttctg tttcaacggt tctgaatgcc gaggtaatga atttcagccg taaaggatcg
     2281 cagggtttcc atgtagccgt aatggctccc atgccattca caaacttgtc gtcttcctga
     2341 ccttcgaaaa agaccctcaa acgataggct tcctgaatgg taccaaatga tgtttcacga
     2401 tcatgaggta ctaagcggaa aagattgatg gaaaagttac ccagtgccga aatttcgaga
     2461 cgtggatgga ggttatagtt cacattagct tgtacatcgg tgaaggaggg tttgtaatct
     2521 cctttggttt gctgagaaga aagaatatac tggttggttt tttgacgaat acccagcaaa
     2581 tagttcaatt tcttgttttt cgagatgcct tctatctgaa atgagccgcc cagcaggctc
     2641 aaggaagcgc tgcctccaaa ctgtgcaggt tttttatatt gtatgtcgag caccgacgac
     2701 atcttatcgc catatttggc ttcgaaacca cccgccgaaa ataagataga agatacaagg
     2761 tcggggttga gaaaactcaa tccttcctgc tgccccgaac gtatgaggaa aggcctataa
     2821 atttcaacat cattcacata aacaaggttt tcgtcgtaat tgcctcctct taccgagtat
     2881 tgcgaactca attcattact ggaagttacg cccggcatcg tcttaatgag agcttcaatt
     2941 ccgccgccaa gggtaggaat ggtagttgcc gcttttggat cgatgcgggt aaggcttgat
     3001 gaacgaattt gatgatcttc gatagaaacg ggagaaagta aggtgttttg aatctccaac
     3061 gtgcgacgaa tagtgatttt ttcaccttga tgaagatgga gaggaatgcg ttcggttttg
     3121 aaacctatga atgaaactat cagattcacc tcgatttcgg ccggaacccg taattgaaaa
     3181 aagccgttcc tgtcagtcga agtcccgcca ggtaatcctt ctatagagat atttacgagt
     3241 tcgaggggtt ctccatgctg attcgatata tagcccgaaa cctgagccat tggctttttc
     3301 tgactgaaaa gtgtatacga ggttaaaaca atactgataa acaggataaa tattcgaaaa
     3361 tcagcaataa ataaaggctt cgttttcaat ttcagtttct gttgaaaata ggtttgcgag
     3421 aggccaaaat taaaagtttg gcaattagtt gccaaacctt tcatctttaa acggcttagt
     3481 tgataaaaaa gtacacattg gtaaacaaaa taaaaagtcg ccgaatttta cagcgacttt
     3541 tttgcaattc actatttttc ttgttttctt tattcaacca cgatgattcc tttgcggctg
     3601 gcatccttta aggcagcata aatacccttc ttatggtcgg tggttttatc attatgtttt
     3661 cggttgtt
//
LOCUS       DAORPD010000049         3645 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_4447_length_3645_cov_2.513649, whole genome shotgun sequence.
ACCESSION   DAORPD010000049 DAORPD010000000
VERSION     DAORPD010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3645
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4447_length_3645_cov_2.513649"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            426..1697
                     /locus_tag="PK976_01010"
     CDS             426..1697
                     /locus_tag="PK976_01010"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HRC66500.1"
                     /translation="MKTKKWIILLSLLFMGIIRVYSQDIEYARFIVKTLTSSEYKGRG
                     YIGKGDKKAAEFIVGQFEKYRLQPFGKDYLQKYSFSINTFPEKMEVVVDDRKLMSGKD
                     YVISLASKGVKASYELEWLIDDSIPESSRWKELCSIDLTHKLVVTDEFHKNLVEVNLL
                     GAAGYVFLKDSNEIISWKVSNGRILKNFLVMDIQKEKLGKTTQRISVEFKNKYEENYQ
                     TQNVIGMIRGLGSPDTFLVISAHYDHLGMMGEKTMYPGANDNASGVGMMLDLARHFSL
                     PGNEPYYSLVFIAFSGEEAGLMGSNYYAEHPLFPLAQIKFLINLDMIGSGSEGIGVVN
                     GQHLKKAGLLLQQLNDKGKYLSEVALRGESKNSDHFPFYNKGVSCFFIYTMGKEWPYY
                     HTPEDTCCPPFTAYIGLFDLLNEFIVKVRFL"
     gene            complement(1959..2855)
                     /gene="gldN"
                     /locus_tag="PK976_01015"
     CDS             complement(1959..2855)
                     /gene="gldN"
                     /locus_tag="PK976_01015"
                     /inference="COORDINATES: protein motif:HMM:TIGR03523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldN"
                     /protein_id="HRC66501.1"
                     /translation="MLKMYRKIAVVILGLSLYCWGSEAQVIEPPKVKPMERPWPKIHT
                     PNRKRPVPYAPVREADVIWAKRIWRVIDLREKQNLPLYYPLRPVRDRKSLASVLFEAV
                     ITPQQENPYGSNYLTAYTDEDLAERYTTVDDVKRRLSFEYRDTYIDSLGNETTQEKVY
                     ELKAEDVVKYFIIEDWFFDKRRSQLDVRIHAICPVYYRFIMSNGQKQYLEDPSPTFWI
                     YYPEARFWLVNNEAYNPKNDAEWRTYDDIFTKRKFASYIYKEENVYDRMISDYASGLD
                     ALLESERIKSEILQFEQDLWEY"
     gene            complement(2862..>3645)
                     /locus_tag="PK976_01020"
     CDS             complement(2862..>3645)
                     /locus_tag="PK976_01020"
                     /inference="COORDINATES: protein motif:HMM:NF023503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GldM family protein"
                     /protein_id="HRC66502.1"
                     /translation="GIDKWLPSYASGASNVTSGELGMAHLKISTAGMDYGPKKFAGVI
                     TILNPLGVEEQYEFADEFVVQEPVAVISLDKMSVLYIGLDNPITVSVPGIPPDNLDIK
                     ISGPAEVKRLSAGKFEIRPTSPGSLKMQVYANIENTSRLMGERTLRLLPVPDPTIVVG
                     GKTAGQSIALADLQNAKVDAVLKGFLFDDVRFVVTRFEVLASIGGLTQSVTVKGNMMN
                     DEAKSMLRKLGSNQQVIISNISVRGPDGKDRPLSTAVNLKTI"
BASE COUNT         1107 a          650 c          744 g         1144 t
ORIGIN      
        1 gtctcgtgtc tctttgtgaa tgattttaac ccctaaaatc ccaatttata cgtaattaaa
       61 taaaggaaga ttgctgtata tgtttgtata cgaccatctt attataattt tgcaattgtg
      121 tatttaggta atttttagca ttttaccttt aaaatttcag aactcatgat ttggtgttaa
      181 tcaggagttc tgaatttata aaaccaaact cactattttt gataaacatt ctatacattg
      241 aatcagtgcc ttaatatcaa gataagggat agattggatc atatagcatg atgtgacatt
      301 cttcaaatct tgaatatatt aaaagtttgg tttttcttct ttggtgtttt tacaaaatgc
      361 tttaatttag ccatgtttta tgaataaaca ttgcaaaaca acttaacact gcaaacagag
      421 agtaaatgaa aacaaagaaa tggattattc ttctttccct gttattcatg gggatcatca
      481 gggtatattc gcaagatatc gaatatgcac ggtttatagt aaaaaccttg acttcgtcag
      541 aatacaaagg acgagggtac ataggcaaag gcgataaaaa agcagcggaa ttcattgtag
      601 gacagttcga aaaatatagg ctgcaacctt tcgggaaaga ttacttacag aaatactcct
      661 tcagcatcaa tacctttccc gagaaaatgg aggtggtggt agatgatcgc aaattaatgt
      721 cagggaagga ttatgtgata tcattggcat ctaagggagt aaaagcctct tacgaattag
      781 aatggcttat cgacgacagc attcctgaaa gtagtcgctg gaaggaatta tgtagtatag
      841 atttaacaca taaactcgtt gttactgatg aatttcataa aaatcttgtt gaggttaatt
      901 tattgggcgc agctggatat gtattcctca aagacagcaa tgaaattatc tcatggaagg
      961 tttctaatgg tagaattttg aaaaattttt tagtgatgga tattcagaaa gagaagttag
     1021 ggaaaacaac tcagagaata tccgttgaat tcaagaataa atatgaagag aattatcaaa
     1081 ctcagaatgt gataggaatg ataagaggat tagggagtcc agatactttt ctagtgattt
     1141 cggcccatta tgatcattta gggatgatgg gcgaaaagac catgtatccg ggggccaatg
     1201 acaatgcgag tggtgtaggt atgatgctcg atttggcccg acatttcagt ttgcctggca
     1261 atgaaccata ctattctctt gtatttattg ccttcagtgg agaagaagct ggattgatgg
     1321 gctcaaatta ttatgcggag catcctttat tcccattagc acaaatcaaa ttcctcatca
     1381 atctcgacat gataggcagt ggaagcgaag gcattggagt agttaatggc cagcatttga
     1441 agaaagcagg cctgttgctt caacaattaa acgataaggg taaatattta tcagaggtgg
     1501 ctctgagggg agaatcgaaa aacagtgacc atttcccttt ttataacaaa ggggtttctt
     1561 gtttctttat ttatactatg gggaaagagt ggccttatta tcatactcct gaggatacct
     1621 gttgcccacc tttcactgct tacataggat tgttcgatct tttgaatgaa tttatcgtta
     1681 aggttaggtt tctttaataa aggttgtcta tgcaaataga tcatttactt tatattagat
     1741 atccaaaagg gcatctacca aaattcaaag tctaaaaagt ttcgatggtc aaggttactc
     1801 ttatggttag agatttgaca taaagtttgc cttgcttcgg tttaaggcgc agacagaagg
     1861 ctatatatga aaaaaatgaa ggggtaaagg gtttttcctg ataataaaaa agcccggttt
     1921 taaaaccagg ctaataaaag ggaagcacaa atgggaaatt aatattccca caaatcttgt
     1981 tcaaactgca gtatctcact ttttattctt tccgattcta agagagcatc taatccagag
     2041 gcataatcgg atatcattct atcgtaaacg ttttcttctt tatagatata gcttgcgaat
     2101 tttcgttttg tgaatatatc gtcgtaggta cgccattctg cgtcattctt tggattatag
     2161 gcctcattgt ttaccaacca gaaacgagct tcagggtaat aaatccagaa agtaggagaa
     2221 gggtcttcga ggtattgttt ttgaccgttg ctcataatga aacggtaata aaccgggcag
     2281 attgcatgaa tacgcacatc gagctggctt cgtcttttat cgaagaacca gtcttctata
     2341 atgaagtatt ttaccacatc ctcggctttc agttcataga ctttttcctg tgtggtttca
     2401 tttcccaatg aatctatata ggtatcgcgg tattcgaaac taaggcgtct tttgacgtca
     2461 tccacagttg tatagcgttc ggctaaatct tcgtcggtat atgcagtcaa gtaattcgag
     2521 ccataaggat tttcctgttg aggagtaata acggcttcga acagtacaga agccagtgat
     2581 tttcgatcac gaacgggacg aaggggataa tataggggta aattttgttt ttcccttaaa
     2641 tcgatgactc tccaaatccg tttagcccat atcacgtcgg cttcacgcac aggtgcataa
     2701 ggcacaggcc tttttcgatt gggtgtatgt atcttgggcc agggcctttc cataggcttc
     2761 actttaggag gctcaattac ttgagcttcg cttccccaac aatataatga aagacctaaa
     2821 ataacaacag caattttgcg atacattttc aacattatat tttaaattgt tttcaagttt
     2881 acggccgtac tcagtggcct atccttacca tcgggtcctc tcactgaaat gttactgatg
     2941 ataacctgct ggttactgcc taattttctc aacatgcttt tagcttcatc attcatcata
     3001 tttcctttta ctgttacact ttgagtaagt ccaccaatgc tggcgaggac ttcgaatcgt
     3061 gttacgacaa atcgtacatc atcgaaaagg aaacctttga ggacggcgtc tactttagca
     3121 ttttgcaaat cagccagtgc tatggattgt ccggcagttt tccctcccac aacgatggta
     3181 ggatcgggta cggggagaag acgcaatgtg cgttcaccca ttaagcgaga agtattttcg
     3241 atattcgcat aaacctgcat ttttaaacta cctgggctag taggtctaat ttcgaattta
     3301 ccggctgaaa ggcgtttaac ttcagcaggt ccagaaattt taatatcgag gttatcgggt
     3361 gggattcctg gtacagagac tgttatagga ttatctaaac cgatataaag aaccgacatt
     3421 ttatcgaggg agataacagc cacaggctct tgaactacaa attcatcggc aaattcatat
     3481 tgctcttcca ctcctaaagg attcaagatg gtaatcactc cagcaaattt tttagggcca
     3541 taatccattc cggcagtgct gatttttaag tgggccatgc ccaattcacc ggaagtaaca
     3601 tttgaagctc cacttgcgta agaaggcaac catttgtcta ttccg
//
LOCUS       DAORPD010000050         3636 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_4465_length_3636_cov_2.517174, whole genome shotgun sequence.
ACCESSION   DAORPD010000050 DAORPD010000000
VERSION     DAORPD010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3636
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4465_length_3636_cov_2.517174"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="PK976_01025"
     CDS             <1..665
                     /locus_tag="PK976_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66503.1"
                     /translation="IAVTCIFAGFSIWSFYHLPLIDFLPWKKGNIVSTVSQQSSEYYV
                     TYRNKTTGEEREFPSNDFPYKDSIWMSQWEFVSSRVVSHPSSDNTLLLISDTLGNDVS
                     LYYLNYPDFLFIATIYDLHSAPDKSLKKLSDFIRSAENSGYTCIILTSALNKEIEDFA
                     LKYDIQAEIFNIDDITLKMMVRANPGLILIKNGVIIDKWNVRSMPDFNKIEKKHLKLT
                     NE"
     gene            662..1204
                     /locus_tag="PK976_01030"
     CDS             662..1204
                     /locus_tag="PK976_01030"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRC66504.1"
                     /translation="MIPRIIIKLNKFTLRPFSWADTTSLANYANDSRVATFLRPSFPY
                     PYTSKDAEEYIYFCLNAQDAFVLAIDINHEAVGSIGVQYIQFVGKSTWELGYWLGVPF
                     WNQGIMSDAVKNFIHYLAFYLNTDEIIAKVYSLNIGSIKVLENNGFKKLNFNETTRIR
                     TGQLVEEWVFMKNLERHIFR"
     gene            1407..2183
                     /locus_tag="PK976_01035"
     CDS             1407..2183
                     /locus_tag="PK976_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66505.1"
                     /translation="MKTLILSDQEASELQEFYMRELARAQRRLSHCQQILSKFESPVE
                     ITEIRPKQVTATNEPIAGEVYTEKKKRGRPRKDQQTAGQAIAAPEAALAAPKKIGRPR
                     KEKPVLEKGETPETEVSKNIPQPFETESLAQEMPTEVVEENIEIQTSKKLKKAGKKAD
                     KVETKSSKKTIKQSEELGIKIPWTSFIMDTLKNATHPLTAKEIANIAIKQYEIPDKKT
                     NYVRNTIAACLSGLATRSKKIDAIATEAKVKAYLLPPSEN"
     gene            complement(2292..3206)
                     /gene="xerD"
                     /locus_tag="PK976_01040"
     CDS             complement(2292..3206)
                     /gene="xerD"
                     /locus_tag="PK976_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HRC66506.1"
                     /translation="MQTQWNEWIKGFRAYLTLEKGLSANTLEAYVHDVQLLTEYFEIT
                     SIVKSPAEVSREDLSRFVEYIYQLGLEASSQARIISGIRAFFKYMLLEGTLNENPAEL
                     LEIPRLQRKLPVVLSEEEIDRLIRIIDVSRPEGQRNKAIIETLYACGLRVSELIELRI
                     SRLYLEEGYIRVIGKGNKERFVPIGEEAIHNIVLYIENVRNTLKVQKQWSDIVFLNRR
                     GGKMSRVMVFLIIKDLAAKAGISKKISPHTFRHSFATHLVENGASLRAVQDMLGHASI
                     TTTEIYTHLDKEYLRRTLLDFHPFYVKK"
     gene            3403..>3636
                     /locus_tag="PK976_01045"
     CDS             3403..>3636
                     /locus_tag="PK976_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019152050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRC66507.1"
                     /translation="MKIICIGRNYKAHVEELHNRLPENPIFFMKPETSLLPRHHPFYF
                     PEFTKELHYECELVLKICRLGKHIDERFALGYVE"
BASE COUNT         1221 a          694 c          623 g         1098 t
ORIGIN      
        1 tcatagcagt aacctgcata tttgccggat tttcgatctg gtcattctac catttacctc
       61 ttatcgattt tttgccttgg aaaaaaggaa atatcgtttc aacggtaagt caacaatctt
      121 cagaatatta tgtaacatat aggaataaaa ccacaggaga ggaaagagaa ttcccttcca
      181 atgattttcc gtataaagac tctatatgga tgtcccaatg ggaattcgtg agttcgagag
      241 tagtaagcca tccttcatca gataatacac tattgcttat tagtgatact ttaggcaatg
      301 atgttagcct ttactacctc aattatcccg attttctttt tattgccaca atttatgatt
      361 tacattcagc acctgataaa tccttgaaaa agttgtctga tttcattcgt agtgcagaaa
      421 attcgggata tacatgcata atactaactt cagctctcaa caaagagata gaggattttg
      481 cactaaaata tgatatccag gctgaaattt tcaatatcga cgatatcaca ctcaaaatga
      541 tggtaagggc aaatcctgga ttaattttaa taaaaaacgg ggtaattatc gataaatgga
      601 atgttcgatc aatgcccgat ttcaataaaa tcgaaaaaaa gcatcttaag ctaacaaatg
      661 aatgattcca agaatcatca tcaaactcaa caaatttacc ttaaggcctt tttcatgggc
      721 cgatacaaca tcactggcaa attatgccaa tgattcaagg gtggcgactt ttttgcggcc
      781 ttcctttcca tatccctaca ccagtaaaga cgcagaggag tatatttatt tctgcctgaa
      841 tgcacaagat gcatttgttt tggccatcga tataaatcat gaagctgttg gttcgattgg
      901 tgttcaatac atacaattcg tgggtaaatc cacatgggaa cttggttatt ggcttggagt
      961 cccgttttgg aatcaaggaa ttatgagtga tgccgtaaag aactttattc attatttagc
     1021 attctattta aataccgacg aaatcatagc aaaagtttac agtttaaata ttggctcaat
     1081 taaggtattg gaaaacaatg gtttcaaaaa actaaatttc aatgaaacaa caagaattag
     1141 aacagggcaa ttagtagagg aatgggtttt tatgaaaaat ttagagcgtc atatttttag
     1201 ataatttatt gattaaaaat ctattatatt ttctttcggt tcaagaaagt taaattaatg
     1261 attttttttt acaaaagatt gcatttttaa aaattattta tatttttgta gcctgaattc
     1321 agatacaagt ttttctttgc aaaacattta tcttattctc tgaaaataaa taaattatca
     1381 ctcaaaacta ttaaacacct taccagatga aaacattaat tcttagtgat caggaagcca
     1441 gtgaactgca agaattctat atgagggaac tggcccgcgc gcaacgcaga ttatcgcatt
     1501 gccaacagat tctcagtaaa ttcgaatcgc ctgttgaaat taccgaaatt aggccaaaac
     1561 aggttactgc tacgaatgaa cccatagcgg gtgaagtata taccgaaaaa aagaaaaggg
     1621 gaaggccaag gaaagatcaa caaactgccg gtcaggccat tgctgcgcct gaagccgccc
     1681 ttgcagcccc taagaaaata ggtcgtccaa gaaaagaaaa acctgttttg gaaaaaggtg
     1741 aaactccaga gactgaagtt tcaaaaaaca tacctcagcc ctttgaaacc gaatcacttg
     1801 ctcaagaaat gcctacagag gttgtggagg aaaacataga aattcaaacc tcgaaaaaac
     1861 taaaaaaagc aggaaagaaa gccgataagg ttgaaacaaa aagctcgaaa aaaacgataa
     1921 aacaaagtga ggagctgggt ataaaaattc cttggacgag ttttattatg gataccctta
     1981 aaaatgccac ccatcctctg actgcaaagg aaattgcgaa tattgccatc aaacaatacg
     2041 aaattcctga taaaaaaacg aattatgtaa ggaatactat tgcagcctgt ctgtcaggtt
     2101 tggctactcg tagtaaaaaa atcgatgcta ttgctaccga agcgaaagta aaagcttacc
     2161 tcttaccccc ctccgaaaat taattttttc cggtgtattg attgttattc acaatttttt
     2221 tgtatctttg tagcgctaaa ataataaata tttttttgac ataggatagg cgcttccttt
     2281 acgggaagcg tctatttttt tacgtaaaaa ggatgaaaat ccagcagcgt tcgacgtaaa
     2341 tactccttgt cgagatgtgt ataaatctca gttgtagtga tggatgcatg ccccaacata
     2401 tcttgtacgg ctctgaggct tgccccattc tcgaccaaat gagtagcaaa tgaatgccga
     2461 aaggtatgtg gacttatttt tttcgaaata cctgcctttg ctgccaaatc tttgattatt
     2521 aaaaatacca ttacacgact catcttgccg cctcttcgat tcagaaaaac tatatcactc
     2581 cactgctttt gaaccttcaa tgtattcctc acattctcga tgtaaagcac tatgttatga
     2641 atagcttctt ctccaatcgg aacgaaacgt tccttgttgc cttttccaat cacacggata
     2701 tagccttctt ccaaatacaa acgcgaaatc ctgagctcga tcagctccga aacacgtaaa
     2761 ccgcacgcat acagcgtttc aatgatggct ttgttgcgtt gcccttcagg ccgactcaca
     2821 tcgataattc gaattagtcg atcgatttct tcttctgata aaactacagg aagtttcctt
     2881 tgtaaacgag gtatttctaa taattcagcc ggattctcat tcaaagtgcc ttcaagcaac
     2941 atatacttga aaaaagcacg aattcctgaa ataattcttg cctgagaact tgcttcaagc
     3001 cccagttgat aaatgtattc cacaaaacgg ctaagatctt ctctgctgac ctcagctggt
     3061 gattttacta tcgaagttat ttcaaaatat tcagttaaaa gttggacatc atgaacataa
     3121 gcttccaaag tattagctga aagacctttc tcaagagtca agtaagccct gaatcctttt
     3181 atccactcat tccattgagt ttgcataagc aaaaataaat cataattatc aaatttgcct
     3241 ctagttttag taaaacagtc aaatcttgta aacaataaac atcattattc ttagatttgc
     3301 aaaccattga attctataga aaattcattt ttcataccga tccgaatgtt ggcgttttgg
     3361 tttactttat catcgattaa agattaaata atcaaactcg aaatgaaaat catctgtata
     3421 ggaagaaatt acaaagcaca tgtggaagaa ttgcacaatc ggcttcccga gaatccgatt
     3481 tttttcatga aacccgaaac ctcccttctg ccgcgtcatc atcctttcta ttttccggaa
     3541 tttacgaaag aacttcatta tgagtgcgag cttgttttaa aaatttgccg tttgggcaaa
     3601 catatcgacg aaagatttgc ccttggttat gtggaa
//
LOCUS       DAORPD010000051         3631 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_4477_length_3631_cov_2.711130, whole genome shotgun sequence.
ACCESSION   DAORPD010000051 DAORPD010000000
VERSION     DAORPD010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4477_length_3631_cov_2.711130"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="PK976_01050"
     CDS             <1..190
                     /locus_tag="PK976_01050"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018357478.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the B subunit is part of the
                     catalytic core of the ATP synthase complex; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HRC66508.1"
                     /translation="LTDYDQRALDFAREYSEYLLAIDVNIDIDKMLDTGWELFARHFN
                     QAEVGIKQELVDKYWPVK"
     gene            254..856
                     /locus_tag="PK976_01055"
     CDS             254..856
                     /locus_tag="PK976_01055"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007761831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HRC66509.1"
                     /translation="MAVKFQYNKTSLQEMNKQLRIRLRALPTLKNKESALRAEVKRAK
                     DYATSLDEKLSAAMAEYNEMVRLWNEFNPGLVVIHDVDLGIKKIAGVRIPILQQIHFE
                     IKPFSLFNQPVWYMDGILILEKLASIAIEREFYYAKMKLLDHARKKTTQKVNLYEKVQ
                     IPGYEEAVIKIKRFLEDEENLSKSSQKILKNRFDQKEVAV"
     gene            883..2658
                     /locus_tag="PK976_01060"
     CDS             883..2658
                     /locus_tag="PK976_01060"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HRC66510.1"
                     /translation="MVYHEDYERFLKGLRSVGVIDIVKRKKELDKDAAERLALQKQVA
                     ETIKLLKKHKKPEITAEAPSEGDGMKIFEHIRDLQAEVESLNQQLATLKKEIEYTEPW
                     GDFDPQIIEKLRKAGLRISFYIIPSRKFDPQWLSKYNIGVVNEQPGVIYFILVNEGQE
                     EIDLPAEEVKAPERPLSELLKKQEAIQTRLSEIEEEFARLAASSIPLLQKTLHQIASE
                     VDYHVVVNNTLSEAEGKLKILEGFAPVETKETVEKYCEENGIFYIETQPDPNEKVPIL
                     LRNNSFAKLFEPIGHLYSLPKYGELDLTPFFAPFFMMFFGFCLGDAGYGLFIFIAATL
                     FKKFKAKPSFKPYVTLAQYLSLATFIFGAISGTFFGISLIDADIPIVENIKSYFFDSN
                     KMLVLSLILGAVQIVFGMCIKVVNITHQEGFRNALSLVGWLILILGLLIRQGLVSAGV
                     LPKGETIVLYSILGVSCLLIFFLNDLKTNVFVRIGKGLWDVYGMATGIFGDLLSYIRL
                     FALGISSSILGIVINNVAMSILGIPYIGPLLFVVFLVIAHLGNIAISSLGAFVHPMRL
                     TFVEFYKNAGFKGGGKQYKPFAEKR"
     gene            2722..3183
                     /locus_tag="PK976_01065"
     CDS             2722..3183
                     /locus_tag="PK976_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007560291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HRC66511.1"
                     /translation="MTTAVILAYVGLAMMLGLAGIGSAIGVTICGNATIGALKKNEEA
                     FGPYMLLSALPGTQGLYGFGGFFIINAKLTPQFIASMTLGQGMAIFAAGLVLGGVGLY
                     SAIRQGQVCANGIAGIGSGYDVFGKTMVLAVFPELYAIISFAATFIISAAL"
     gene            3265..3630
                     /locus_tag="PK976_01070"
     CDS             3265..3630
                     /locus_tag="PK976_01070"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HRC66512.1"
                     /translation="MEIKPTVVLGVSRNPERMANQAVEALLNHGHPVYAIGKEDFIWG
                     KIVVHSTWDQAVDIHTVSIYLRPELQKVHQQRILSVNPQRIIFNPGTYNPELAIAASE
                     RGIEILEECTLVLLSSGQF"
BASE COUNT         1089 a          665 c          750 g         1127 t
ORIGIN      
        1 ccttacggat tacgaccagc gtgcactcga ttttgccaga gaatattccg aatatcttct
       61 ggctattgat gtaaacatcg acatcgataa aatgctggat acaggctggg aattgtttgc
      121 acgccatttt aaccaggccg aagtgggcat aaaacaagaa ctcgtcgata aatactggcc
      181 tgtaaaataa aacacaatat gtaaagaatg gaaaattttc caatgataaa gctataaaag
      241 taatgagtag aaaatggcgg taaaatttca atataataag acttctcttc aggagatgaa
      301 taagcagctc cggatacgcc tgcgagcatt gcctacttta aaaaacaagg aatcggcatt
      361 aagagcagaa gtgaaacgag ccaaggatta tgccacaagc ctcgatgaaa agttgtctgc
      421 tgctatggcc gaatacaatg aaatggtgcg tttatggaat gagttcaacc ccggattggt
      481 tgtcattcat gatgttgact tggggattaa gaaaattgcc ggagtgcgta tccctatcct
      541 tcagcaaatc catttcgaaa taaaaccctt cagcctcttc aatcaaccag tttggtacat
      601 ggatggcatc cttattctcg agaaacttgc ctctattgct atcgaaagag aattttacta
      661 cgccaagatg aaattgctcg atcatgcccg taaaaaaact acccaaaaag taaatcttta
      721 cgaaaaagtt cagattccag gttatgaaga agctgttatc aaaataaaac gcttccttga
      781 agacgaagaa aacctttcca aatcttcgca gaaaatcttg aagaatcgat ttgaccaaaa
      841 ggaggttgcg gtatgatagt ccccatgacc aaattcattt ttttggtata tcatgaagat
      901 tatgaacgat ttctaaaggg cttgcgatct gtgggtgtta tagatatcgt aaagcggaag
      961 aaggaactcg acaaagacgc agctgaaagg cttgccttgc aaaagcaggt tgctgaaacc
     1021 atcaagttgc ttaaaaagca taaaaaaccc gaaatcacag ctgaagcgcc ctcagaaggg
     1081 gatggaatga aaatatttga acacattaga gatttacagg cagaggtcga gtctttaaat
     1141 cagcaactcg ctacattgaa aaaggaaatt gaatatactg aaccctgggg agatttcgac
     1201 cctcaaatta tcgaaaaact tcgtaaagca ggattgagaa tatcttttta tatcatacct
     1261 tctcgtaaat tcgatcctca gtggttatcg aaatacaata ttggggtggt gaatgaacag
     1321 ccgggtgtta tttatttcat tcttgtgaat gaaggtcagg aagagattga tttgccggcc
     1381 gaagaagtta aagcacccga acgtcctctg agtgaattgt taaaaaaaca ggaagctata
     1441 cagacacgcc ttagcgagat tgaagaagaa tttgcaaggc tggctgcgag ctctattcct
     1501 cttttgcaga aaacattgca tcaaattgcc tccgaagtcg attatcatgt agtagtaaac
     1561 aacactttat cggaagcaga agggaagttg aagattttag aaggttttgc ccctgttgaa
     1621 acaaaagaaa ccgttgaaaa gtattgcgaa gaaaatggta ttttttatat cgaaactcag
     1681 ccagatccaa acgaaaaggt tcctatcctc ctgcgcaaca atagtttcgc gaaattattc
     1741 gagcccattg gccacttata ttctttaccc aagtatggcg aactggatct tactccattc
     1801 tttgcccctt tctttatgat gttttttggc ttctgcttag gtgatgctgg ttacggattg
     1861 tttatattta ttgctgctac cttgtttaag aaattcaaag ctaaaccttc attcaaacct
     1921 tatgtaactc ttgcacagta tcttagttta gctaccttta ttttcggagc aatttcggga
     1981 accttcttcg gtatcagtct tatagatgct gatattccca ttgtggaaaa tatcaaatcc
     2041 tatttcttcg atagtaacaa gatgctggtg ctctctttga tacttggagc agtgcaaatt
     2101 gtattcggaa tgtgtatcaa agttgttaat attactcatc aagaagggtt tcgaaatgca
     2161 ctttcattgg ttggctggtt gatccttatt ttgggacttt taatccgtca aggattggtt
     2221 tcggccggcg ttctacctaa aggagaaacc atcgttctat atagtatttt aggagtttcc
     2281 tgcttattga tctttttcct aaatgattta aaaaccaatg tttttgtgag aattgggaaa
     2341 ggtttatggg atgtatatgg aatggcaacg ggtatatttg gcgatttact ctcttatatt
     2401 cgactttttg ccttgggaat ttccagttcc atacttggaa tagtaatcaa taacgtggct
     2461 atgtctattc ttggaattcc ctatatcgga cctcttcttt tcgtggtatt ccttgttatt
     2521 gctcacttgg gcaatattgc cattagttct cttggcgctt ttgttcatcc tatgcgtcta
     2581 acctttgttg aattttacaa gaatgctggt ttcaaaggag gaggaaaaca atataaacca
     2641 tttgccgaga aacgataatt tatagtctat gcgacaaatc agattcatca ataaaccaat
     2701 catttaaaac ttttttcgat tatgacaact gcagtaattt tagcttatgt aggacttgcc
     2761 atgatgcttg gattagcagg tattggcagt gctattgggg taactatttg tggtaatgcg
     2821 accatagggg ctctgaaaaa aaatgaagaa gcctttggtc catatatgtt actgagtgct
     2881 ttgcccggaa cacaaggttt gtacggtttt ggaggtttct tcatcatcaa tgcaaaactt
     2941 acaccccaat ttattgctag tatgacttta ggccaaggta tggccatttt tgctgctggc
     3001 cttgtacttg ggggtgtagg cttgtattcg gctatacgtc agggacaagt atgcgccaat
     3061 gggattgctg gaataggttc gggttacgat gtttttggta aaacaatggt tttggccgtt
     3121 ttccctgaac tttacgccat catttcattt gcagctactt ttatcatcag cgcagctttg
     3181 tagtattttt accaaagtat tatcgtattt cctcccaact ggttatggat gacttctcct
     3241 taatcagttt ttcattcacc aattatggaa ataaaaccta ccgttgttct tggggttagc
     3301 cgaaaccctg aacgcatggc taatcaggcg gttgaagctt tattaaacca tggacatcct
     3361 gtttatgcca ttggaaaaga ggattttatt tggggtaaaa ttgtggttca ttcaacctgg
     3421 gatcaagcag tagatataca tactgtttct atctaccttc gaccggaatt acaaaaggta
     3481 catcagcaaa ggattctatc agtgaatcct caaagaatta tctttaaccc aggaacatat
     3541 aatcctgaat tagcaatagc tgcttctgag agaggtatag aaattttgga agaatgtaca
     3601 cttgtattgc tttcttcagg ccaattttga a
//
LOCUS       DAORPD010000052         3567 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_4595_length_3567_cov_2.126139, whole genome shotgun sequence.
ACCESSION   DAORPD010000052 DAORPD010000000
VERSION     DAORPD010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3567
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4595_length_3567_cov_2.126139"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="PK976_01075"
     CDS             <1..334
                     /locus_tag="PK976_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66513.1"
                     /translation="IYSWTARTTFNKEELSAGGIFVVEHTIAEKNYLKANHRLLFDLS
                     ENSGGKMVWPVQTDSIPQWLKKMGNTATKVYTEESYVKLNDLGWLLALMIILISGEWA
                     IRKWSGIL"
     gene            370..1665
                     /locus_tag="PK976_01080"
     CDS             370..1665
                     /locus_tag="PK976_01080"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRC66514.1"
                     /translation="MNTTVIYLFYAFLAILILSFAAERILDWLNIRNWPKVLPDLIRD
                     IFPEEKFMEARQYQLVNYKVGFIEATITSIITIGFLYFGGFAWLDGVVSSFSSNLIVQ
                     SLIFFGIIAVATFIIRLPFDIYDTFVIESRFGFNTTTPRIYITDQLKSALLTILIGVP
                     LFSLIVFLYQKTGEYFWVLVWLVISIFALFMSLFYSSLIVPLFNKQIPIPEGELRTTI
                     EEFAHKAGFKVKDIYVIDSSKRSSKANAYFTGLGPEKRIVLYDTLIKDFSIDEILAVL
                     AHEIGHYKHHHTTIGLITSIVQTGLILFVFSLFVKPGSELTEVLCNAAAGARQVTPSF
                     YMGLIGFTLLFSPISAIIGLIFNSISRRHEFQADAYVAEHQMQGYLQSALKKLSVNHL
                     SNPFPHPLYVKVYYSHPPLIERLKALENTSRREPQGNGF"
     gene            complement(1887..>3567)
                     /locus_tag="PK976_01085"
     CDS             complement(1887..>3567)
                     /locus_tag="PK976_01085"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HRC66515.1"
                     /translation="LDLVFSGKIWIDTKNYALRRLDLEITSSVNINFIDKYKIQQDYE
                     RQSTGHYLPTKTRVLVDLQQTSDSAVGFIGKYYIANKDYIINQPRPPSFYDRNVEVDM
                     AAKTYSDDFWNEQRKEMIADHEQVKSSLEAIDSLKKAPQITFIRRALRIIWDGYYNFG
                     KIELGHWYTMFGNNPIEGFRFQTSVRTNINWNYYWILSGYGAYGFKDETFKYRLQVER
                     FLNRSKWEKIGLRYSYDMMRLGVDPEFLESHVFLNYIFLFSSQFGYLHRMSLSQDARI
                     WYETDHWRGWNTKLMIRTKTFHSQGDYYFGYYDDNDLIRTKYRTSEFHLITSYSKRRT
                     WLVENNWRVGAEALKSQLWTFHATLGFKNVLGSDFTFQKFSLNVMHKWRTGYFGQLDY
                     SLTGEVTLGKVPYPEAIIPQGNEPVFAAEKAFNMMNFFEFVADKSISGILLHHFDGLF
                     FNRIPLLKHLKWREVAGFNFVFSTYGKKNYIRTNDNPKGLLAPSINGNPITEFRIMDF
                     GKPYMEVSYGIENILKIIRVQAFHRLSYLDPGPDGKKIPKFMIKASFFIRL"
BASE COUNT         1015 a          627 c          782 g         1143 t
ORIGIN      
        1 aatctattca tggactgcca gaaccacctt caacaaggaa gaattatcgg ctggggggat
       61 ttttgttgta gaacatacaa ttgccgaaaa aaactactta aaggctaatc atcgtcttct
      121 tttcgatttg agtgaaaata gcggaggaaa aatggtctgg cctgttcaga ccgatagtat
      181 tccccaatgg ttgaaaaaaa tgggaaatac cgctactaaa gtctataccg aagaatctta
      241 tgttaaactg aatgatttag ggtggctttt ggcattgatg atcatcctca tatcgggaga
      301 atgggcaata aggaaatggt cgggaatatt atgatcagat gaggcgaata aaaatttaga
      361 tcaataaata tgaatactac tgttatttat ttgttttatg ctttcttggc tattttaata
      421 ctgagttttg cagccgaaag gatcctggac tggttgaaca ttaggaattg gcccaaggta
      481 ttacccgatc ttattcgaga tatatttccg gaagagaaat ttatggaagc ccgacaatat
      541 cagttagtaa attataaagt aggattcatc gaagcgacga ttacttctat cattaccatt
      601 gggtttctat attttggtgg atttgcatgg ctggatggag tagtaagttc cttttcatca
      661 aatttaatcg tacagtctct gatttttttt ggaattatag ctgtagccac atttatcatc
      721 aggcttccgt tcgatatcta tgataccttt gtgatagaat cgcggtttgg attcaatacc
      781 actactccca gaatctatat tactgaccag ctaaagtcgg cattgttgac catcttgatc
      841 ggagttccat tgttttcgct tattgttttt ctttatcaaa agaccggaga atatttttgg
      901 gtactggttt ggttggtgat tagtattttt gcattattta tgtcgttgtt ttacagtagt
      961 ttgatagtgc ctttattcaa caagcaaatc cccatacccg agggagaatt acgcactacc
     1021 atcgaagaat ttgcccataa agcgggattt aaggtgaagg atatttacgt gatcgacagt
     1081 tcgaagagga gcagtaaggc caatgcttat tttactggcc ttggacccga aaaaagaatc
     1141 gttttgtatg atacgctgat aaaagatttt agtatcgatg agattctggc tgttttagct
     1201 catgaaattg gacattacaa gcatcatcat accacgatag ggcttataac gagcattgtt
     1261 cagactgggt taatattgtt cgtattttct cttttcgtta aacccgggtc ggaattgacg
     1321 gaagttttat gcaatgctgc agcaggtgcc aggcaagtaa ctccttcatt ttatatggga
     1381 ttaattggct ttactctttt gtttagccct atctcggcca tcatcggatt aatcttcaat
     1441 tctatatcac gcagacatga atttcaggcc gatgcctatg tagcagaaca tcaaatgcag
     1501 ggctatttgc aatcggctct taaaaaatta tctgtcaatc atttgagtaa tccctttccg
     1561 catcctttgt acgtaaaagt gtattattct catcctccat tgatagaaag actgaaggca
     1621 ttggaaaaca ctagtcgaag agaacctcaa gggaatggtt tttagaaata gaagctacgg
     1681 gcttaatttt tcggaagaaa gattggctaa gtacaaatac tgagataata tgatttctac
     1741 tttctcattt aattcttagc ccgaatttgc catgagggta gagattcaat ataagaaaga
     1801 ataggggaaa catcgagaat tattttcttt tttcgaggtt tcacagtttc gaagaattac
     1861 atagctttat ataaaatagg ggagggttac aatcggatga agaaactggc cttgatcatg
     1921 aatttaggta tttttttgcc atcgggaccc gggtcgaggt atgataatcg atgaaaggcc
     1981 tgaacacgta tgattttgag gatgttttct ataccatatg aaacttccat gtagggtttg
     2041 ccaaagtcca tgatacggaa ctctgtgatt ggatttccgt tgatagaggg agccaataaa
     2101 ccttttggat tatcgtttgt acgtatataa tttttctttc cataggttga gaagacgaaa
     2161 ttgaatcctg ccacttctct ccatttgagg tgtttgagca gcggaatacg gttgaagaac
     2221 aggccatcga agtggtggag taaaattcca ctgatggatt tatcggcaac gaattcgaaa
     2281 aaattcatca tgttgaaggc tttctcagca gcgaatacgg gttcattgcc ttgagggata
     2341 atggcttcgg gatagggaac ttttcccaga gtaacttctc cggttaatga gtagtcgagt
     2401 tggccgaaat aaccagttcg ccatttatgc atgacattta gggaaaattt ctggaaagtg
     2461 aagtcactgc cgagaacatt tttaaatccc aatgtagcat ggaaagtcca taattgtgat
     2521 ttaagtgctt cggcaccaac gcgccagttg ttttccacca gccatgttct tcttttcgaa
     2581 tagctggtaa taagatgaaa ttcggaagta cggtattttg tccgaatgag gtcattatcg
     2641 tcgtaatagc caaaatagta atcaccttga gaatggaagg ttttggttct tatcatcaat
     2701 tttgtattcc agcctcgcca gtggtcggtt tcgtaccaga tacgggcgtc ttgcgagagg
     2761 ctcatcctgt gaagatatcc aaactgagaa gaaaagagga agatataatt gaggaataca
     2821 tgggattcga gaaactccgg gtcgactccc aggcgcatca tatcatacga ataacgtagt
     2881 cctatctttt cccatttgct gcggtttaag aatcgttcta cctgcaaacg gtatttgaaa
     2941 gtttcgtctt tgaatccata agcgccatag cctgaaagta tccagtagta gttccagttg
     3001 atattggtcc ttactgaagt ttgaaatcga aacccttcga tggggttatt gccaaacatg
     3061 gtgtaccagt gcccaagttc tatctttcca aagttgtagt agccatccca gatgattcga
     3121 agagctcttc gaataaatgt aatttgaggg gcttttttga gtgaatcgat tgcctcgagc
     3181 gaacttttta cttgttcatg atcagctatc atttctttgc gctgctcgtt ccaaaagtcg
     3241 tcggaatacg tcttggctgc catatccact tcaacgtttc tatcgtaaaa tgaaggaggt
     3301 cggggttgat tgatgatata atctttgttg gcaatatagt atttaccgat gaagcctaca
     3361 gctgaatcgc tagtttgttg cagatcgacc agaactcttg ttttggtagg cagatagtgg
     3421 cctgtagatt gtcgttcata atcttgttga attttgtatt tatcgatgaa gttgatattc
     3481 acggagctgg taatttccag atcgaggcgg cgtagggcgt aatttttcgt atcgatccat
     3541 attttccctg aaaaaaccag atcaagg
//
LOCUS       DAORPD010000053         3559 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_4608_length_3559_cov_2.180365, whole genome shotgun sequence.
ACCESSION   DAORPD010000053 DAORPD010000000
VERSION     DAORPD010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4608_length_3559_cov_2.180365"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="PK976_01090"
     CDS             <1..285
                     /locus_tag="PK976_01090"
                     /inference="COORDINATES: protein motif:HMM:NF037202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66516.1"
                     /translation="GRELTPEVILSCIYAVFYSIIYREKYTELLKIDFSRVPFPKDYK
                     VFSKMAALVNELKDLHLMQSGRLDKLVSKYGGESDRIDMIVYRDSERRFI"
     gene            1165..2748
                     /locus_tag="PK976_01095"
     CDS             1165..2748
                     /locus_tag="PK976_01095"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HRC66517.1"
                     /translation="METKRAFLCSLILFFFLYSAEMRGQDSLSSNLIIQYEFNGDGTN
                     IVNTKYYLKIGDETKNYDFKWMPGRDGSERSALLFTGNSNGLKTSLNISPQILPELTY
                     IAWVYDRPDGFLFGSTLPVNSEKKFSSRSLQFSKGTVIAGFNKLDTLKKPYFASLQTS
                     KLKKDKWNFIALTVSSVDSVLRLYVNNEYFESKGVCYTPNNSFLIIGNTVNSPYTRPY
                     RGRVDEIRVYNKALTAAEISLISGFSISNAQERLERNATLKKALIAGLIAFLFLSVVY
                     MIVVLITERRYRPIKVSSFRSDSNKAPIDRKTEEANKLASDCVENAFETWETVSEEGG
                     QVLKSPTKHKHFKTTYKALAEAKSYNPDDIDVINRMNELGNLLNKLSKRKFYGNWFLA
                     ILTLIFPIVYFLIERNHLDTKGMIGVGVMLLPALAYILSNFAPNYVVANRTNKVGGIF
                     GKVIAFIMGAAASAVAVDYYTETTWSDGSKTTEYDTGSNAISLVIWVLLIIAAILLSM
                     ILAIIAAIIAFFRNYIFYI"
     gene            2901..>3559
                     /locus_tag="PK976_01100"
     CDS             2901..>3559
                     /locus_tag="PK976_01100"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HRC66518.1"
                     /translation="MTKLSVNINKIALLRNARGGNLPDVVKAAMDCERFGAHGITVHP
                     RPDERHIRYSDVRAIKLVLTTEFNIEGYPIPKFLDLVLEVKPAQVTLVPDPPDALTSN
                     AGWDTIKNESFLREVVTELKNHGIRTSIFIETDARMIENAAKIGTNRIELYTERYAAS
                     YLMDAPKAIQPYVEAAQVALKCELGINAGHDLNLQNLKFFTQNIPGLLEVSIGHALIC
                     DA"
BASE COUNT         1118 a          617 c          669 g         1155 t
ORIGIN      
        1 ggtcgtgaac ttacgcccga agtaattctg tcctgcattt acgctgtttt ttacagcatt
       61 atttatcgtg agaaatatac cgagcttttg aaaattgatt tctcgcgtgt gcctttccca
      121 aaagattata aggttttcag caaaatggct gctttggtga atgagttgaa agatttgcat
      181 ttgatgcaaa gtggacgatt agataaactt gtatcaaaat atgggggtga aagtgatagg
      241 attgacatga ttgtttatcg tgattcagaa aggcgattta tataaacgat gaaaaatatt
      301 ttgataatgt acagcctgaa gtttggaact atgagattgg aggatatcag gagctttata
      361 aatttctgaa agataggaaa ggaagaaata tatatgaggc tgtgaattat tgcaaaatca
      421 ttaccgcatt gagcctcaac attataacac agcaagaaat tggcaagacc tatcccgaag
      481 ttgaaaagag gatcatcgaa ttttaaaggg aaagttttta gactatccca tacagaagaa
      541 tttaaaatcc aaaaagataa gattactctt gatagcaatc gttaagcggg ttggtatttc
      601 attgctttca tccgtgttaa aatgatgata tcagaaaagg ggattcttga cgcttcatag
      661 atcataattt ggtaagaggt gcccctgaaa cctaattata attttcggct atacaatgtt
      721 ttatgccaac tactaaaaaa aatttcagaa atttcataat aaatatttgt aaacagaaaa
      781 gaattcatac gtttgtggtt tctttcgaaa aattgttttt tgaggtgaaa tagattgaag
      841 ataaaaattc aaaagatcta ttctttccta taatagacaa atcagaacat tacaataaat
      901 atttcaaata ttataaaatt atattatcct ttttcaactt attgtaaatt attaaattaa
      961 ttgtaatcta caaattggtt tcttaaaatt tttcttagtt ctttaaaaat cttttaattt
     1021 tattttttgt catagcatta ttttatttat tttttttgat ctgtctatca aattttaaca
     1081 tgaatttttt atattaacga atgacatttt tatacaattt cttcaaaaaa taaacatctt
     1141 tattaaattt aaataatttt ttatatggaa actaaacgag cttttttgtg ttcactgata
     1201 ttatttttct ttttgtattc tgctgaaatg agggggcagg atagtttatc gtccaacttg
     1261 atcattcaat acgaattcaa tggagacggt acgaatatcg ttaacaccaa gtattatctc
     1321 aagattggtg atgaaacgaa aaactacgat ttcaaatgga tgcccggacg agatggatcg
     1381 gaacgttcgg ctcttttatt tacaggcaat tcgaatggat tgaaaacatc cctgaatatt
     1441 tcgccacaaa tattacccga gcttacttat atagcctggg tttatgatcg tccggacgga
     1501 tttcttttcg gatcgacact tccagtaaat tcggaaaaaa agttttcttc tcgttcgctt
     1561 cagttttcga aaggaactgt cattgccgga ttcaataaat tggatacttt gaagaaaccc
     1621 tattttgctt ccttgcaaac ctcgaaacta aaaaaggaca aatggaattt cattgctcta
     1681 acggttagtt cagtcgattc tgtattgcgt ctttacgtca ataatgaata tttcgaatcg
     1741 aaaggagtat gttacactcc taacaatagc tttttaatca ttggaaacac tgtgaattct
     1801 ccttacacca gaccttatcg gggtcgtgtg gatgagattc gagtttataa taaagctctt
     1861 acagccgctg aaatttcact catcagtggc ttttcaattt caaacgcaca ggaacgatta
     1921 gaacgaaatg ctacgctcaa aaaggcatta atagctggct tgattgcttt tcttttcttg
     1981 tcggttgtat atatgatagt agtgttgata accgaaagac gttatcggcc cattaaagta
     2041 tcctcatttc ggagcgatag caacaaggca cctattgatc gaaaaactga agaggcgaat
     2101 aaattggcat ctgattgcgt tgaaaatgca ttcgaaacgt gggagaccgt gagtgaagaa
     2161 ggcggtcagg ttttaaaatc tcctacaaaa cataagcatt ttaaaaccac ttacaaagct
     2221 ttggctgagg caaaatcata caaccctgat gatatcgacg tcatcaaccg aatgaacgaa
     2281 ttgggaaacc tacttaataa actaagtaag aggaagtttt atggcaactg gtttttggct
     2341 attttaacgt tgattttccc cattgtttat tttttaatcg aacgtaatca tctcgataca
     2401 aaagggatga taggagtggg agtaatgtta ctaccggcac ttgcttatat tttgtcaaat
     2461 tttgctccca actatgttgt ggccaatcgc accaacaaag tgggaggcat tttcggcaag
     2521 gtcattgctt ttattatggg tgcggcagca tcggcagttg ccgtcgatta ctataccgaa
     2581 acaacatgga gtgatggaag taaaacgact gaatacgaca caggctcaaa tgccatttct
     2641 cttgttattt gggttttact cattattgcg gccatcctac tttctatgat tttggcaatt
     2701 atagctgcca tcattgcttt tttccgcaac tatatttttt acatttagag ggtgagcttt
     2761 ttgttatgac caaaattctt ttatactcga gaattttacc tcaaaacgtt tttcccacac
     2821 taagggtcta aaacttccgt catgttttgc ctggagagat taacagtatt tttgtaaaat
     2881 caattggata gttcatcgat atgacaaaat taagtgtaaa catcaacaaa attgcccttc
     2941 ttagaaatgc tcgtggaggc aatcttccgg atgtagtaaa agcggctatg gattgtgaac
     3001 gttttggtgc acatggaatt acagtacacc cccggcccga tgaaaggcac atccgctaca
     3061 gtgatgtcag agccataaaa ctcgtcctta ccaccgagtt caatatcgaa ggatacccaa
     3121 ttccaaaatt tctggatttg gtactcgagg ttaaaccggc tcaggtaaca cttgttcctg
     3181 acccacctga tgcgcttacc tccaatgctg gatgggatac gataaaaaac gaatccttcc
     3241 tcagggaagt agttactgag ttgaaaaatc atggaattcg cacctccata tttatcgaaa
     3301 ctgatgcacg catgatagaa aatgctgcca agattggcac caatcgcatc gagctataca
     3361 ccgaacgtta tgctgcctcg tatcttatgg atgcccctaa agctattcag ccttatgttg
     3421 aagcggcaca ggtggccttg aagtgtgaat taggaatcaa tgctggtcat gatctcaatc
     3481 ttcagaattt aaagtttttt actcaaaaca ttccgggcct ccttgaggtt tcgattggtc
     3541 atgccctaat ttgcgatgc
//
LOCUS       DAORPD010000054         3544 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_4643_length_3544_cov_2.887074, whole genome shotgun sequence.
ACCESSION   DAORPD010000054 DAORPD010000000
VERSION     DAORPD010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4643_length_3544_cov_2.887074"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(370..1536)
                     /locus_tag="PK976_01105"
     CDS             complement(370..1536)
                     /locus_tag="PK976_01105"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HRC66519.1"
                     /translation="MKQNVFDPIRSFRDEEVHSYLSKIIDEPLFHEALAYQFGKEKVP
                     YWLDKITTFQSIHELQQELLLPLLENLLRESVEKLTYSGTEALHKNTSYLYISNHRDI
                     LMDSFILNYILFIEGFDTIESAIGNNLFVMPWIEHLARLNKSFVVKRNLEGKALYEAS
                     LILSLYMKDTLFRRQQSLWIAQQEGRSKNGDDFTQPSLIKMLLMASDKGEESNLLNQY
                     HIVPVSVSYEYDPCDVYKCWDAYQKSIGKSFEKTNNMRLNEMKEGLIEFKGKVHFSVT
                     HPMIFLFQEFKMREAIQHVATAIDNEIQSHYIIYPFHWYCYDKSNYSDRYYNEYAGKE
                     NDFETRINHQLQKIEKFFTQTNEEDIMRSYLYGFYANIVKNYLAKTKSASCLNF"
     gene            complement(1743..>3544)
                     /locus_tag="PK976_01110"
     CDS             complement(1743..>3544)
                     /locus_tag="PK976_01110"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC66520.1"
                     /translation="GAFGGGEVIYSNNSGETWQTVTNNLPNSYPFNDIEIAGHTVYLG
                     GGQLFSSQHLGLYTCKLGEFAWQNISSNFPLKVVAKVLVSPVDSLKIYAGTDGDGVYF
                     STDGGLNWDYSSTGASNFSITDMIFYPNNPEEILIGCKSLAVYRSQNAGINWENYSNG
                     IATLPINDIAVDPLDCNNIIVAFEALNSGGCYMSNDGGNTWNPVSGLPATRFSAVTYD
                     IHGYVYAVSRGPSSVAQEGIYKSTDGGITWTNTGPNIGPLFETELWTIEQTPDQPGLL
                     FIGGNHFGVAGYAATIYRTLDAGQSGWEEVYIGADQMKIHALEIAPFSDEQLIYAGYV
                     VYGGNGSLLKSTDQGNHWVEINNGLPNNCCQTYAIAVDPSNPQKVFAGSGYYAAGYYI
                     VKTEDGGENWSPIISLPSQVNCLASIEIPIIKNREKISLDGDPRIIYAGTSSNGVFLS
                     DDDGMSWVDASEGLPANTYVSNFSDPFLTGVELKINASTLGHSGYSTTIAILEGINEL
                     SKEDVLTWNEPNPFSQHTTIHFTLKKNSMVRLSIVNMNGQVIATLLDQKMSSGEHQFI
                     WNAASHGKKLARGLYFYRLEVDGKNYFNKLIVQ"
BASE COUNT         1072 a          712 c          679 g         1081 t
ORIGIN      
        1 attttgggac acccaaaatt acaaaattaa tattgattat caatatatta tatcggtgtc
       61 ccgaaaaagt gcggaaaaac aagaaaaagt ctgtgagtta ttgaacgaca cagaaaccat
      121 tttccccggt acaaatatga cacttgtctt caaaactgcg acaacctgat taacaccgaa
      181 tcaggagttc tgagtttctt caccgaaata aaacgagtga aaatcaacga taaatggtat
      241 accgctgaaa ttaccaagaa aactaataac cttttgaaaa aattaggact attattacgt
      301 aaattttgcg aagttaacga tcaaactttc agtctaaaag aatcctattc tgaaaacaat
      361 gtctctcgtt taaaaattga gacaggaggc tgatttggtt ttcgccagat aattcttgac
      421 gatatttgcg tagaagccat agagataact tcgcatgata tcttcttcgt tggtttgtgt
      481 aaagaatttt tcgatttttt gcaactgatg attgatgcgg gtttcaaaat cattttcctt
      541 accagcatat tcgttataat aacgatcgga ataatttgac ttgtcgtaac aataccaatg
      601 aaaggggtaa attatgtaat ggctttggat ttcgttatcg atagcagtgg ccacatgttg
      661 gatagcttct cgcattttga attcttggaa taaaaaaatc atgggatggg taacagaaaa
      721 atgtacttta cctttgaact cgatcaaacc ctctttcatt tcgttgagtc gcatgttgtt
      781 tgttttttcg aaagactttc cgatagattt ttgataggca tcccagcatt tatagacgtc
      841 gcaagggtca tattcgtatg aaaccgatac aggtacaata tgatactggt tcagcaggtt
      901 cgattcttca cctttatcgg aagccatgag gagcatttta atcaatgaag gttgggtaaa
      961 atcgtcgccg tttttactac gtccttcctg ctgggcaatc cataacgatt gctgccgtct
     1021 aaataacgta tccttcatat atagagacag gataagcgaa gcttcgtaga gcgccttgcc
     1081 ttccaaattt cgtttaacca cgaagctttt gttcaggcgg gctagatgct caatccaggg
     1141 catcacaaac agattgtttc caatggcaga ttcaatggta tcaaagcctt cgataaagag
     1201 gatgtaattt aatatgaagc tatccatcaa aatatctcta tggttggaaa tatagagata
     1261 ggaagtgttc ttgtgaagag cttccgtacc actgtaagtc aatttttcga ccgattcccg
     1321 caacaaattt tccagcaggg gtagaagcaa ttcctgctgc aattcatgga tcgactggaa
     1381 ggtagtgatc ttatccaacc agtaaggaac tttttctttt ccgaactgat aggctaaagc
     1441 ttcatgaaat aatggctcat cgatgatttt ggaaagatag gaatgcactt cctcatctct
     1501 aaatgagcgt atgggatcga atacattttg tttcatgcgg cgaaattaat aagattattg
     1561 aaacacatca tcatttgatt tgagtcggcc ttcaaaatcc acgccagaat gagtaatgac
     1621 agagaattca atagtgaaat aatttctctg aaaattttaa aaaagatatt cctccattca
     1681 aattcgaaaa ggggagacta agctccccct gaatgttcca ttatgtatcg tatcaaatat
     1741 ttttactgta cgattaattt attgaaataa tttttaccat ctacttcaag gcgatagaaa
     1801 taaagtcctc gcgctaattt tttaccatgg ctcgcagcat tccatataaa ctggtgttct
     1861 cccgagctca tcttttgatc gaggagggtg gcaattactt gaccattcat atttacgata
     1921 gacagacgca ccattgagtt ctttttcagt gtaaaatgaa tggttgtgtg ttgagaaaat
     1981 ggattgggtt cgttccaggt aagcacatcc tctttgctta attcattgat gccttccaga
     2041 attgcaattg tcgtactgta gccgctatgg ccgagggtgc ttgcatttat tttcaattcg
     2101 acacctgtta agaaaggatc ggaaaagttc gaaacgtaag tgtttgctgg aagtccttcg
     2161 ctggcatcca cccacgacat tccatcatcg tcagatagga acactccgtt ggatgaggtt
     2221 ccagcataaa taattcttgg atctccatcg agagaaatct tttcccgatt cttgataatt
     2281 ggaatttcaa tagaagccaa acaattcact tgagaaggta aggagattat tggactccaa
     2341 ttttcacctc catcttctgt ttttactata taatatcctg ctgcataata accacttccg
     2401 gcaaaaacct tttgtggatt cgaaggatca acagctatag cataagtttg gcaacaatta
     2461 ttgggcaagc cattgttaat ttctacccag tgatttccct gatcggtgct tttcaacaaa
     2521 gatccattcc ctccataaac cacataaccc gcatagatca attgttcatc gctaaagggc
     2581 gcaatttcga gtgcatggat cttcatctga tctgcaccaa tataaacttc ttcccaaccc
     2641 gattgtccag catcgagggt tctataaata gtagctgcat aacctgcaac tccaaagtga
     2701 tttccaccta tgaaaagcaa tccgggttga tcaggggttt gttcgattgt ccagagttct
     2761 gtttcgaata atggcccgat attgggtccg gtattcgtcc aagtaatacc tccatcggtt
     2821 gatttataga taccttcctg agccaccgat gaagggcctc ttgaaacggc atacacgtaa
     2881 ccatgaatat cgtaagttac ggccgagaat cttgttgcag gaagacccga taccggattc
     2941 catgtatttc cgccatcatt cgacatataa caacctcccg aattcaaggc ctcgaatgcg
     3001 acgataatgt tattacagtc gagtggatcg acggcaatgt cattgattgg aagtgttgca
     3061 atgccattac tgtaattttc ccagtttata cccgcgtttt ggcttctata tacagccaaa
     3121 cttttgcatc caattaatat ttcttcagga ttattagggt aaaatatcat gtcggtaatc
     3181 gagaaattag aagctccggt agaagaataa tcccaattga gaccaccatc tgtcgaaaaa
     3241 tataccccat caccatcggt tcccgcgtaa atcttcaaag aatctacagg gctaacgagt
     3301 acttttgcaa ctaccttcaa aggaaagttg gatgagatat tctgccatgc aaattcaccc
     3361 agtttgcatg tataaaggcc taaatgctgg ctcgaaaaaa gttgtccacc tcctaaataa
     3421 actgtatggc ctgcaatttc gatatcattg aaagggtatg aattgggaag attatttgta
     3481 actgtttgcc atgtttcgcc cgagttattc gagtaaatta cttcacctcc tccgaatgca
     3541 ccat
//
LOCUS       DAORPD010000055         3529 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_4678_length_3529_cov_2.162637, whole genome shotgun sequence.
ACCESSION   DAORPD010000055 DAORPD010000000
VERSION     DAORPD010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4678_length_3529_cov_2.162637"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..1225)
                     /locus_tag="PK976_01115"
     CDS             complement(56..1225)
                     /locus_tag="PK976_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009038820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HRC66521.1"
                     /translation="MPSKIVFFLFLLPIILLSRSVNGQNIIIPGAWKTDSYFSSLGGD
                     RAIGVVANASSMIGEKHLIDSMISAGINVKKIFAPEHGFRGEAEAGEFIQNTIDEKTG
                     LPIISLYGNQKKPSSKEMSGLDVIVFDIQDIGVRFFTYISTLHYMMESCAKEGIPLLV
                     LDRPNPNGDYIDGPVLDLSFRSFVGMNPIPIVYGMTIGELARMINGEGWLRNGMKCEL
                     IVIPLDGYTHSTPVILDREPSPNLPNTQSIRLYPSLCLFEATDISVGRGTDFPFQVIG
                     FPAPIFGNFTFVPQAIPGKSIHPLHKGDTCYGIDLRRDSLHTRFTLKFILDFYHLSNW
                     GEKFFKRPDFFDKLAGSSLLRSQILDGLDENQIRESWQPQLQEFMLKRKPYLLYP"
     gene            1552..1746
                     /gene="rpsU"
                     /locus_tag="PK976_01120"
     CDS             1552..1746
                     /gene="rpsU"
                     /locus_tag="PK976_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006045671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HRC66522.1"
                     /translation="MIIVPVKEGDSIDRALKKFKRKFEKTGVMRELRERQKYTKPSIR
                     RREEKTRAIFIQKLRDQELI"
     gene            1836..2723
                     /locus_tag="PK976_01125"
     CDS             1836..2723
                     /locus_tag="PK976_01125"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRC66523.1"
                     /translation="MIESFITYLEREKRYSKYTCTSYSIDLQQFESYCQLQYGHSDID
                     TVSSDVIRSYVVWLIEKDYSHRSIHRKIISLRQYYRYLIRLGYIHANPARDVPVPKIP
                     ERIPMFVSEENMKNGGNVASKRKNDYETIRDKLIIEMLYATGMRVSELIHLRICDINL
                     EGMNIKVMGKRSKERLIPIGEKLADHLRQYLEVLRRFGFVASPESWLFSTRKGQKIYP
                     LLVYRVVNEFLSKVTTLEKRSPHILRHSFATQLLNAGADLNAIKELLGHSSLASTQIY
                     THSDFETLKKIYQQSHPKA"
     gene            2740..3039
                     /gene="raiA"
                     /locus_tag="PK976_01130"
     CDS             2740..3039
                     /gene="raiA"
                     /locus_tag="PK976_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009138111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HRC66524.1"
                     /translation="MNVNINSVHFKADKKLESFITEKVGKLNQYFDGIIGSEVVLKLE
                     NTDTPENKVTEIRLQIPGNDLYARKQCATFEEATGEAVDALKVQLKKYKAKLKGN"
     gene            3167..3248
                     /locus_tag="PK976_01135"
     tRNA            3167..3248
                     /locus_tag="PK976_01135"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3201..3203,aa:Tyr,seq:gta)
     gene            3265..3337
                     /locus_tag="PK976_01140"
     tRNA            3265..3337
                     /locus_tag="PK976_01140"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3298..3300,aa:Gly,seq:tcc)
     gene            3347..3421
                     /locus_tag="PK976_01145"
     tRNA            3347..3421
                     /locus_tag="PK976_01145"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3379..3381,aa:Thr,seq:ggt)
BASE COUNT         1145 a          658 c          721 g         1005 t
ORIGIN      
        1 gtatggcaaa atacactgca agaattccaa aaaccaaggt agggctcata actaattaag
       61 gatatagcaa atagggttta cgtttgagca tgaattcctg taattggggc tgccagcttt
      121 cacgtatctg attttcatcg agaccatcga gaatctgcga cctcaataac gaagatcctg
      181 caagcttgtc gaagaaatcg ggacgtttga aaaatttttc tccccaattc gataaatgat
      241 aaaaatctaa tatgaatttc agtgtgaatc gagtatgtaa agaatcccgc cgaagatcta
      301 ttccatagca agtatcgcct ttatggagag gatgaataga ttttcctgga attgcttgag
      361 gtacaaaagt aaagttacca aaaatgggag cagggaaacc tataacctga aatggaaaat
      421 cagttcccct ccctaccgag atatcagtgg cttcgaacag gcaaaggctg ggatagagac
      481 gtatgctttg cgtattggga aggttcggac tgggttcccg atcgagaatt acgggtgtgg
      541 aatgcgtata tccatctaaa gggattacaa tgagttcaca tttcattccg tttcttaacc
      601 atccttctcc gttgatcatt cttgccagtt cgccaatcgt catcccataa actatgggaa
      661 taggattcat tcctacaaat gaacggaaag ataaatcgag cacagggcca tctatataat
      721 ctccattggg attgggcctg tcgagaacca acaatggaat accctctttg gcacagcttt
      781 ccatcatata atgaagagtt gaaatataag taaaaaatct aacccctata tcttgaatat
      841 cgaacactat gacatcgaga ccactcattt ccttgctcga aggttttttc tggttaccat
      901 acaaagaaat aattggtagt cccgtttttt catcaatagt attttgaatg aattccccag
      961 cctcggcctc tcctctgaat ccatgttcgg gagcaaaaat tttttttaca ttaattcctg
     1021 ctgaaatcat gctatcgata agatgctttt cgcctatcat ggaagaagca tttgcaacta
     1081 ctcctatggc tcgatcaccg cctaacgaag aaaaatatga atctgttttc catgcgccgg
     1141 gaatgattat attttgacca ttaaccgatc tgcttaacaa aatgataggc aacaaaaaga
     1201 gaaagaaaac tattttagaa ggaataggct tcattatgtg gcaaaattat aaagaacgat
     1261 acataatgaa caacgaatca cggatcgcat tcactcctaa tggggctcga ataatgcggg
     1321 tttgtcttcg aaaatgttga catgccgccc aaaatgatgc tcgaacgaaa ttttttcatc
     1381 ttcatataac tcagtccccg ctatctctca tcctccattt tacccattca caagaagcca
     1441 cgaataacta tagatatcat caatcaacat tctcacattg ctcaaccaat aaaaattgta
     1501 taactttgca gccctaattt ttattcacta attaaaagcg aggtattttt tatgattatt
     1561 gttcctgtta aagaaggcga cagcatcgac cgagcgttga agaaattcaa gcggaagttc
     1621 gaaaaaaccg gtgtgatgcg tgaattacgc gaacgccaaa aatatacgaa accttccatt
     1681 agacgacgcg aagagaaaac tcgagccatc tttattcaaa aactcaggga tcaagagttg
     1741 atttaagatt cttgaaatta caggaagaaa actatccgga cagaaatttt tttctatccg
     1801 gatttgttgt aattttatcc caagctaaat tttggatgat agaaagtttt atcacatatc
     1861 tggaaagaga aaaacggtat tcaaaataca catgtacctc ttattcgatt gatttacaac
     1921 aattcgaatc ttattgtcaa ttgcaatatg gccactcgga tatcgatact gtaagttctg
     1981 atgtgatcag aagttatgtg gtttggttga tagaaaagga ttattctcat cgaagcattc
     2041 accggaagat tatttcgttg cgtcagtatt acaggtattt gattagattg ggatatattc
     2101 atgctaatcc ggctcgagat gttcctgtac caaagattcc agaacgaatt cccatgtttg
     2161 taagtgaaga aaacatgaaa aatgggggta atgttgcgtc taagaggaag aatgattatg
     2221 aaaccatacg ggataaatta atcatagaaa tgctatatgc cacaggaatg cgtgtttcgg
     2281 aattgattca tctaaggatt tgcgatataa atcttgaggg gatgaacatc aaagtgatgg
     2341 gaaagcgaag caaagaaaga ttgattccta ttggagaaaa attagcagat catttacgac
     2401 aatatctcga ggtgcttcga cgttttggtt ttgtagcttc tcctgaatcg tggttatttt
     2461 cgacccgaaa aggccagaag atttatcctt tgcttgttta tcgagtagta aatgaattct
     2521 tgagtaaagt aactacattg gaaaagagga gtccacatat tctgaggcat agttttgcca
     2581 cacagttatt gaatgccgga gccgacctga atgccatcaa ggaattgctg ggtcattcta
     2641 gtttggcgtc cacccaaatt tatacacatt ctgactttga gacgttaaaa aaaatatatc
     2701 aacaatcaca ccctaaagct taaaagaaag gagtaaatca tgaatgtaaa catcaactca
     2761 gtacatttca aggccgacaa gaagctggaa tcatttatca cagagaaggt tggaaaattg
     2821 aatcaatact tcgatgggat cattggaagt gaggtcgtat tgaagttgga aaataccgac
     2881 acacctgaga ataaagttac ggaaatcagg ttgcagatac ctgggaatga tctatatgca
     2941 cggaagcaat gtgccacatt tgaggaagca acaggagaag ccgttgatgc tttgaaagtc
     3001 cagttgaaaa aatataaggc caaactcaag gggaactaaa aattttgttc aaaaagtttg
     3061 gttcgtatcg taataagatt tacttttgca gtcccttttt tgggactgtt tttttatagc
     3121 ccgtgatggg gggtttaaaa attttttaat gatagaataa agctgagggg agattccaga
     3181 gcggtcaaat ggggcagact gtaaatctgt tggcagtgcc ttcggaggtt cgaatcctcc
     3241 tctccccacc gaatgaagaa acaggcggaa gtagctcatc tggcagagcg aaagccttcc
     3301 aagctttagg tagcgggttc gaatcccgtc ttccgctctt agaaaagccg atgtagctca
     3361 gcggtagagc acttccttgg taaggaaggg gtcacgagtc caattctcgt catcggctcc
     3421 aacaatgaca cgttcatctt tgataacttt tttcaactaa taaatttttt ctatggcaaa
     3481 agaaaaattc gatcgttcca aacctcatgt gaatattgga acaattggt
//
LOCUS       DAORPD010000056         3508 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_4713_length_3508_cov_2.456125, whole genome shotgun sequence.
ACCESSION   DAORPD010000056 DAORPD010000000
VERSION     DAORPD010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4713_length_3508_cov_2.456125"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="PK976_01150"
     CDS             <1..467
                     /locus_tag="PK976_01150"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HRC66525.1"
                     /translation="LPRRFAAALTLKQQVDSLLHQGEANILIMGDLNDNPFDISVKDV
                     LQACIDTANCESSLINLMAPLMKTQNAGSHKFQGQWDIIDNIIVSKTLYEGKLHLRAR
                     KAKIFAPPFMLTKDKNYTGKKIYRTYNGMKYEGGYSDHLPVITFLAVKRPEI"
     gene            complement(509..1489)
                     /locus_tag="PK976_01155"
     CDS             complement(509..1489)
                     /locus_tag="PK976_01155"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HRC66526.1"
                     /translation="MANLNTNYMGLNLRSPIIAGSSGLTSSLKNIVEIERQGAGAVVL
                     KSIFEEQIRFEAHQAHSFQQSPYPEAWDYIHQYTQSHELDKYLRLIFDAKENTSFPII
                     ASINCITAAQWTSFARQIEQAGADALELNIFILPSDPFRSSEENEKLYFDIAKAVLRE
                     LNIPVSIKLSWYFSSLTRTFLQLSWTGIKGMVLFNRFYSPEIDIDTLEVTSGHVFSQP
                     GELNLPLRWIAMLSDRMHCDLAGSTGVDSGEEALKLLLVGAKAVQMVSALYRYGFSRI
                     ADTNQQIGSWMDQRGYESLEQIIGKMSLKNIENPASYERVQFMRHFAGVE"
     gene            complement(1846..2547)
                     /gene="radC"
                     /locus_tag="PK976_01160"
     CDS             complement(1846..2547)
                     /gene="radC"
                     /locus_tag="PK976_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HRC66527.1"
                     /translation="MKKKEESKSIHQWAEDDRPREKMLKKGPDSLSNAELLAILISSG
                     NIEESALDLAKRILQDHQNNLTALSRLELSDLMKYKGIGMAKAVTIKAALELGKRRLS
                     EEAFEQGKIQSSKDAYLIFRKYLEDNHYERFYALFLNKANSPLGEPILIGEGGTDGTV
                     VDPKRIMRLALEKFASSLIIAHNHPSGNLQPSAADINLTEKIAAAGKLLDIVLLDHLI
                     IGINNYYSFAEARKL"
     gene            complement(2814..>3508)
                     /locus_tag="PK976_01165"
     CDS             complement(2814..>3508)
                     /locus_tag="PK976_01165"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HRC66528.1"
                     /translation="TGIMPPKKRNGHFVKPFDPLSTHNQGFIEGNAWTYSLFVPHDID
                     GLIQLMGGANKLEAYLDTLFTMSLPEEYYENTEDITKDGVMGNYVHGNEPGHHIPYLY
                     NYTSHPWKTGLQVEKILKTFYRNSPSGLCGNDDCGQMSAWYIFSSLGFYPICPGSTEY
                     TIGRPAFEEINFQLENGHNIHIVTKNFRSDIPYVQQVLLNGKPLSTPFIHHSDLKDGA
                     QIEFIMGKSPKI"
BASE COUNT         1068 a          776 c          630 g         1034 t
ORIGIN      
        1 cactccctcg gcgctttgcc gccgcgctta cacttaaaca acaagtagat tctttgcttc
       61 atcaaggcga agccaatata ctgattatgg gcgatttgaa tgataatcct ttcgacattt
      121 cggtgaaaga cgtgcttcag gcctgcatag atacagcaaa ctgcgaatca tctcttatca
      181 atctgatggc tccattgatg aaaacgcaaa atgcaggtag tcataaattt caaggtcaat
      241 gggatattat cgacaatata attgtttcga aaactttata cgaaggcaaa ttgcacctga
      301 gagcccgaaa agcaaaaata tttgctcccc ctttcatgct gacaaaagac aaaaattata
      361 ctgggaaaaa aatctatcgt acttataacg gcatgaaata cgaaggtgga tatagcgatc
      421 acttgcctgt cattaccttt cttgcggtaa aaagacctga gatataaatg atcgaataaa
      481 gatttcatca ataagaatca tgaaaaatct attctactcc tgcaaaatga cgcataaatt
      541 ggacacgttc gtatgaagca ggattttcta tatttttcag actcatttta ccaataatct
      601 gttcaagtga ttcatatcca cgctggtcca tccaagaacc aatttgctgg ttagtatcgg
      661 caatacgtga aaaaccataa cgatagagag ccgacaccat ttgtacggct tttgcgccaa
      721 ccagtaagag tttcaaagcc tcttcgccac tatccacacc cgtcgaaccc gccaaatcgc
      781 aatgcattct gtcgctcagc atggcaatcc agcgcaaagg gagattgagc tcaccaggtt
      841 gactgaacac atgcccgctg gttacttcca aggtatctat atcaatctcg ggactgtaaa
      901 aacgattgaa aagtaccatc cctttgattc ctgtccatga taactgtaaa aacgtcctgg
      961 tcaaactcga aaaataccag ctcagcttaa tgcttacagg aatattcagt tccctgagta
     1021 cagcctttgc tatatcgaaa taaagtttct cgttttcttc actactcctg aaagggtcgg
     1081 aaggaaggat aaatatattt aattcgagtg catcagcacc cgcttgttcg atctgccggg
     1141 caaaactcgt ccactgagca gcagtaatac agttaatgct tgcaatgata gggaaagacg
     1201 tattttcctt agcatcaaaa atcaatcgaa gatatttatc gagttcatgc gattgagtgt
     1261 attgatgaat ataatcccat gcttcaggat aaggcgattg ctgaaaagaa tgggcctgat
     1321 gcgcctcaaa cctgatttgt tcttcgaaaa tggatttcaa tacaaccgca cccgcgccct
     1381 gccgctcgat ctcgacgatg ttctttagag aagaggttaa tcctgaacta ccagctataa
     1441 tagggcttct caaattcaag cccatataat tcgtattcaa attggccata tcacgaaaat
     1501 tttaatcttg atttttcgaa ccacaaacct aaataatttt cccttcgttt ggatgatttc
     1561 ggcaaacttc attctcaatg aatgttcgaa ccattgaaat tatcattcct gcgaatccat
     1621 caaataattc agacagacaa tcgaattccc ttatctgatg agatttccgt gctttctttt
     1681 tgacaagaag attttcttga tcccactcga ctaaatcgaa aacataaggc aacaaactct
     1741 tattcaggaa ggtaaattca cattcaacac atcgaagaac ctccacaaat acgtttcttt
     1801 ttcaaacaac cattcctttc gagaaatatg ccatgaagca gaatttcaaa gttttcgtgc
     1861 ttcggcaaaa ctgtaataat tgttgattcc aataataaga tgatcgagaa gaacaatatc
     1921 gagcaactta cctgcggcag caatcttttc ggtaagatta atatcggcgg cacttggttg
     1981 aagattccct gagggatgat tatgtgcaat gatgagagaa gaagcaaact tttcgagggc
     2041 caaccgcatg atgcgcttgg gatcaactac cgttccatcc gtccctcctt cacctatcaa
     2101 aatcggttcg cctaaaggac tgttggcctt gttcaagaac aatgcataaa atcgctcata
     2161 atgattatct tctaaatatt tcctgaaaat cagataagca tccttgctcg actgaatttt
     2221 cccctgttca aaggcttcct cggagagtcg gcgcttgcca agctccaaag cggcctttat
     2281 ggtaacggcc tttgccattc caattccttt atatttcatc aaatccgata attccagtct
     2341 ggaaagagcc gttaaattgt tctggtgatc ctgtaagatg cgctttgcca aatctaaagc
     2401 actctcttca atgttacctg aagatattaa aatagccagt aattctgcat tgctcaatga
     2461 atcgggtccc tttttcagca tcttttcccg aggccggtcg tcctctgccc attgatgaat
     2521 actcttactc tcttccttct ttttcataag attttcgttt tgccgaagta aaattaaata
     2581 ctttactcga ttcctatggt tacatcttta ggggattaaa aatttccaaa atcctcatca
     2641 tactgaaatc tccgtctata aaaattttca gcctgctcga attgtgcatt cacatcaatc
     2701 ccaagctaca aatcctcaca ttagctccag aatcaaaatg aattgcattc ttttatcttt
     2761 ctatttattt gatacttcag aatcccagat ggattcaaat catcgaaaac ttttcaaatt
     2821 ttaggactct tccccatgat aaattctatc tgagctccat ctttcaaatc agaatgatga
     2881 ataaatgggg ttgaaagggg tttgccgttg aggagaacct gctgtacata aggtatatca
     2941 gaccgaaaat ttttcgtaac gatatgaata ttatgaccat tctcaagctg gaagttaatt
     3001 tcctcaaaag caggcctgcc tatggtatat tcggtgctac ccgggcaaat cggataaaaa
     3061 cccaatgaac taaagatata ccaggccgac atctgaccgc aatcatcatt gccacaaagg
     3121 cctgatggag aatttctata gaatgttttc aaaatttttt cgacctgcaa tcctgttttc
     3181 caaggatgcg aagtgtaatt atacaaataa ggaatatggt gcccgggttc atttccatgg
     3241 acataatttc ccattacccc atctttagta atatcttcag tattttcgta atattcttcg
     3301 ggcaacgaca tggtgaacaa cgtatcgaga taggcttcga gtttatttgc gccgcccatt
     3361 agctggatta agccatcgat atcatgaggt acaaagaggc tgtaagtcca ggcattcccc
     3421 tctataaatc cctgattatg cgtcgacaga ggatcgaaag gtttcacgaa gtgtccatta
     3481 cgttttttag ggggcataat tccggttt
//
LOCUS       DAORPD010000057         3481 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_4765_length_3481_cov_2.457677, whole genome shotgun sequence.
ACCESSION   DAORPD010000057 DAORPD010000000
VERSION     DAORPD010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3481
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4765_length_3481_cov_2.457677"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1193
                     /locus_tag="PK976_01170"
     CDS             <1..1193
                     /locus_tag="PK976_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66529.1"
                     /translation="WPHNVKKLLRQLNVPIIPIHFSGYNSLLFYSLGLINPKLRSLRL
                     PAEVLTKSGKVIKVTIGKAIHPKEYINLSLNDFGDILRTKIYSLDYSFSNKIEYRATN
                     LVYNNEKSFTNDSLVQSEINKIEKELILQLDDFQFYFTQSNKILYSVRSFLKSKEKIY
                     YTNKFKNNIATIDELYNESRYAFVWDRKNSTIVYACRIDLGKEIIENHRMKCIFINNV
                     FKLNTEVKSLVNNSILFDELYFANDYRGNNIFYITLWESIFEIFRNLKYHYLISLFHI
                     PYIYQKETTSLFINFLKENFFDNTFSEHIKSKNRSTLPFPKRLSKKAFLDFTGKNFNK
                     LEEFLTDLEPTFEIDPIICQKLYLMHSTILGLNINTKKSSYIDTLFLTDITMVPENFR
                     QLLE"
     gene            1250..>3481
                     /gene="ileS"
                     /locus_tag="PK976_01175"
     CDS             1250..>3481
                     /gene="ileS"
                     /locus_tag="PK976_01175"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HRC66530.1"
                     /translation="MSKKYREYKCLDLSKICNEILNVWDQDHTFQKSLEKTKTGEPFI
                     FYEGPPSANGVPGIHHVMARTIKDIVCRYKTLKGYYVERKAGWDTHGLPVEIEVEKKL
                     GITKDDIGNKISIVDYNNECRKEVMKYKNLWDELTIKMGYWVDLEHPYITFDNKYIES
                     VWYLLLQLYHKGLLYKGYTVQPYSPAAGTGLSTHELNQPGCYKMVKDNSVIAQFKIIK
                     DEKSLFLFRLTQYEIFCLAWTTTPWTLPSNTGLAVGENISYSLVETYNPYTGLAEAVI
                     GATQLLPTILDFRNKDLDFDSYKPGDKKIPFRIVAEFLGKELEGIRYEQLLPYAKPKD
                     GDPFRIVLGDFVSTVEGTGIVHIAPSFGADDFKVGKQYNLGYLTLVDKKGKFTSEMGE
                     FAGRYVRPEYEPDGDHSRGPVDIEIIVKLKKENKAFRSEKYEHSYPHCWRTDKPILYY
                     PLDSWFIKTTAMKDKMIEENDKILWKPQSTGTGRFGNWLENLVDWNLSRTRFWGTPLP
                     IWRTKDNQEEICIGSVEQLFLECEKSVESGFMKVNPFKDCKNASTGKIDYNSFDLHRP
                     FVDEIILLSPLGKPMYRESDLIDVWFDSGAMPYAQFHYPFEREDELDLFFPADFIAEG
                     VDQTRGWFYTLHAIAVMIFGKAAFKAVVSNGLVLDKEGNKMSKRVGNVVNPFTTIENY
                     GPDATRWYLITNSNPWDNLKFDENGIIDVSRKFFSTLFNTYAFFALYANIDNFQYTEE
                     DMPI"
BASE COUNT         1193 a          551 c          651 g         1086 t
ORIGIN      
        1 catggccgca caacgtaaaa aaattgcttc gacaattgaa tgtaccaata attcctattc
       61 acttttcagg atacaattca ctgttattct attctttagg cttaatcaat ccaaaattga
      121 gaagtttaag attacctgca gaagttctta caaaatcggg gaaagtaatt aaagtaacca
      181 ttgggaaggc cattcatcca aaagaataca taaatttatc tcttaacgat tttggagaca
      241 ttttgagaac aaaaatatac agtttggatt attcatttag caataaaatt gaatatcgag
      301 ctacaaactt agtttataat aatgaaaaat catttacaaa tgactcctta gttcaaagtg
      361 agataaataa aatcgaaaag gaattaattc tgcaattaga tgattttcag ttctatttca
      421 cacagtctaa taaaattctt tattctgtaa ggagtttctt gaagagtaaa gaaaaaatat
      481 actatactaa taaatttaaa aataatatcg ccaccattga cgaattgtac aatgaatcgc
      541 gttatgcctt tgtttgggat agaaaaaatt ctacaatagt ttatgcttgc agaattgact
      601 taggcaagga aattattgaa aatcaccgta tgaaatgtat ttttataaat aatgttttta
      661 aattgaatac agaagtaaaa tcattagtaa ataattcgat actatttgat gaattgtatt
      721 ttgctaacga ttaccgggga aataatattt tttatataac attatgggaa agtatatttg
      781 aaatttttcg aaacctgaag tatcattatt tgatttcttt gtttcatatt ccttatattt
      841 accagaaaga aaccacatca ttatttataa actttttgaa agaaaacttt tttgacaata
      901 ctttctcgga acatatcaaa agtaaaaatc gttcgacttt gcctttcccg aagcgattgt
      961 cgaaaaaagc gtttttagat tttactggaa agaattttaa taaactggaa gaatttttaa
     1021 cggatttaga acctacattc gaaatagatc caataatttg ccagaaacta tatctcatgc
     1081 attccaccat actgggatta aatatcaata caaagaaatc gagttatatc gatactttat
     1141 ttttaaccga tatcactatg gttcctgaaa attttcggca attgttggaa tgaaattgat
     1201 aattaaaaac tcaattgtag cttttcaaaa gatttaataa ataaaatcta tgagtaagaa
     1261 atatagagaa tataaatgtt tagatttaag caagatatgt aatgaaattc taaatgtttg
     1321 ggaccaagat catacctttc agaagtcatt ggagaagaca aaaacgggag aaccatttat
     1381 tttttatgag ggccctcctt cggccaacgg agttccaggt attcatcatg taatggctcg
     1441 aacaatcaaa gatattgtat gtaggtataa gacattaaaa ggctattatg ttgaaaggaa
     1501 ggccggttgg gatactcatg gcttgcctgt tgaaatagaa gtagaaaaaa aacttggaat
     1561 taccaaggat gatataggga ataaaatatc cattgtcgat tataacaatg aatgccggaa
     1621 ggaagtaatg aaatataaaa atttatggga cgaattaacc ataaaaatgg gctattgggt
     1681 tgatttggaa catccctata taactttcga taataaatat atcgaatcag tttggtattt
     1741 attattgcag ctttaccata aagggttatt atataaaggt tataccgttc aaccgtattc
     1801 acctgctgct gggacaggct tgagtaccca tgaattaaat cagcctggat gttataagat
     1861 ggtgaaagat aactccgtta ttgcacaatt taaaatcatt aaggacgaaa agtccttatt
     1921 ccttttccgg cttactcaat atgaaatttt ttgccttgcc tggacaacta ctccatggac
     1981 tttgccttct aatacaggct tggccgtggg tgagaatata agttatagtt tagttgagac
     2041 ctataatccg tacactggac tagccgaagc ggtaattggt gcaacccagt tgctaccaac
     2101 tattctggat tttaggaata aggatctgga ttttgattct tacaaacctg gtgataagaa
     2161 aatacctttt agaattgtcg cagagttctt gggcaaagag ctcgaaggta ttcggtatga
     2221 acagctattg ccttatgcca aaccaaagga tggggatccc tttcgtatcg tattaggcga
     2281 tttcgtctcg actgtggagg gaaccgggat cgtacatatt gcacccagtt ttggagccga
     2341 tgactttaaa gtagggaaac aatataattt gggctattta accttggttg acaaaaaggg
     2401 aaaatttact tctgaaatgg gagaatttgc tggtagatat gtaagaccag aatacgaacc
     2461 cgacggcgat cattccaggg gcccggtaga tattgaaatt atagtgaaac tcaaaaaaga
     2521 gaataaagct ttcaggtcgg aaaaatacga acatagctat ccacattgct ggcgcaccga
     2581 taaacccata ctttattacc cactcgattc atggtttatt aaaactacag ccatgaagga
     2641 taaaatgatc gaagaaaacg acaaaatttt atggaaacct caatcaaccg ggacgggtcg
     2701 gtttggcaac tggctcgaaa acctcgttga ttggaatttg tcgagaacga gattttgggg
     2761 aactccgctt cctatctggc gtacaaaaga taaccaagag gaaatctgta taggttcagt
     2821 agaacaactt ttcctggaat gtgaaaaatc tgtcgaatcc ggttttatga aggtaaaccc
     2881 ttttaaagat tgtaaaaatg cctctacggg taaaatcgat tataattcat tcgatcttca
     2941 tagacctttt gtcgatgaaa ttattttgct ttcccctttg gggaagccaa tgtacagaga
     3001 gagcgatcta atcgatgtgt ggttcgattc aggtgcaatg ccttatgctc agtttcatta
     3061 tccatttgaa agagaagatg agttagactt gtttttccca gctgatttca ttgccgaagg
     3121 tgtagatcaa acacgaggat ggttttatac gcttcatgcc attgccgtga tgatttttgg
     3181 aaaagccgct tttaaggctg ttgtttcgaa tggtctagtc ctcgataagg aaggaaacaa
     3241 gatgtcgaaa cgtgtcggca acgttgtgaa tccatttact acaattgaaa attatgggcc
     3301 agatgctact cgatggtatc tcataaccaa ttcgaatcct tgggacaatt tgaaattcga
     3361 tgagaacgga attatagatg taagcagaaa attcttcagt acattattta atacctacgc
     3421 tttctttgct ctttatgcca atatcgataa tttccaatac accgaagagg atatgccaat
     3481 c
//
LOCUS       DAORPD010000058         3458 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_4809_length_3458_cov_2.520129, whole genome shotgun sequence.
ACCESSION   DAORPD010000058 DAORPD010000000
VERSION     DAORPD010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4809_length_3458_cov_2.520129"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1072
                     /locus_tag="PK976_01180"
     CDS             <1..1072
                     /locus_tag="PK976_01180"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005468979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HRC66531.1"
                     /translation="LNEAIANRGHVLQGGKLMDEQKYLHPNDDVNKSQSSNDTFPTAM
                     HIAAYKAVVEVTLPGMQKLRDTLNEKAKQYKNVVKIGRTHFMDATPLTLGQELSGYVS
                     QLDHCMVAIKNTLKHLSELALGGTAVGTGLNTPKGYDVLVAKHIAKLSGHPFVTAKNK
                     FESLATHDAIVETHGALKTAAVALMKIGNDIRMLASGPRCGIGEILIPENEPGSSIMP
                     GKVNPTQSEALTMAMAQVMGNDVAINIGGMSGHFELNVFKPVIIYNFLMSARLIGDAC
                     MSFDEHCAVGIEANLPRIKQNLDNSLMLVTALNTKIGYYKAAEIAKKAHKEGLTLKQA
                     AMALGYVSDKDFDKWVKAEDMI"
     gene            complement(1211..1369)
                     /locus_tag="PK976_01185"
     CDS             complement(1211..1369)
                     /locus_tag="PK976_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66532.1"
                     /translation="MWLKSQINDNYFKKAYLSSISEMRQKTLENPRGFCGLDGTRTRD
                     LRRDRAAF"
     gene            complement(1449..2270)
                     /locus_tag="PK976_01190"
     CDS             complement(1449..2270)
                     /locus_tag="PK976_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005935476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HRC66533.1"
                     /translation="MNLIEPFGYVFFQHALLASVFSAIICGFIGTYIVSRRMVFISGG
                     ITHASFGGMGLAYFFGFNYLLGAAVFALVSALVVEYLSKRTEVREDSAIGMLWSLGMA
                     IGIIFTFLSPGYAPNLMSGLFGSILAVSNTELWLMAVLAIIIILFFIQFFPAILAVAF
                     DMEYASTHHIPVNFINYSLISLIALAIVLNIRVSGIILVLAMMTIPPVTANLLTKRLK
                     PMILLSIALGLAGNVLGLIFSYLLNLPSGATIIVTHILIFGIVRLCISLRETHTA"
     gene            complement(2257..3045)
                     /locus_tag="PK976_01195"
     CDS             complement(2257..3045)
                     /locus_tag="PK976_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HRC66534.1"
                     /translation="MTRNKLIEISDLSAGYYGKPVIYHIHLTVYEGDFIGIIGPNGGG
                     KTTFIKTIAGIIKPLSGMLIFYSPEGQAIEKPKLGYLPQVNTFDKSFPISVEEVVLTG
                     LLSAKGRLRRFRANDKEAVHEVLERLKISHLASKPIGELSGGQMQRVFLARAIVSQPW
                     ILLLDEPSTYADNRFEAELYELLVQLNEKMTILMVSHDLGMIPSYIKSIACVNGTLHY
                     HPTPEITPEILRVYNCPIELIAHGIVPHRVLSTHQENSSTNESH"
     gene            complement(3103..>3458)
                     /locus_tag="PK976_01200"
     CDS             complement(3103..>3458)
                     /locus_tag="PK976_01200"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HRC66535.1"
                     /translation="EAYIHQRLDSVSQHSFMIFHPSLTYLARDFNIQQIAIEERGNSP
                     STRRMKEIVELARSKGITMILYQSQFDSGNAATIAKEIGGTAVPFDPMAYDWYQNMIK
                     LTDLLYQGLNPSNKK"
BASE COUNT         1070 a          787 c          722 g          879 t
ORIGIN      
        1 cctcaatgag gcgatcgcca acagaggaca cgtattacag gggggcaagc tgatggacga
       61 acaaaaatac ctccacccca atgacgatgt aaataagtcg cagtcatcca acgatacctt
      121 ccctacggcc atgcacattg cggcttacaa agcagtagtc gaagtgacct tgcctggtat
      181 gcaaaagcta agagataccc tcaacgagaa ggccaaacaa tacaaaaatg tggtgaagat
      241 tggtcgtacc cacttcatgg atgccacccc acttaccctg ggtcaggagc tcagcggcta
      301 cgtttcacag ctcgaccatt gtatggtggc gatcaaaaat accttaaagc acctttctga
      361 actggccctt ggtggtacag ccgtaggtac aggtctcaac acccccaaag gttacgacgt
      421 actggtggcc aaacacatcg ccaaactcag cggtcaccct tttgtgacag ccaaaaataa
      481 gtttgaaagc ctggctaccc acgatgccat tgtagagacc cacggagccc tgaaaaccgc
      541 ggctgtagcc ctcatgaaaa taggcaacga catccgcatg ctggcttccg gccccaggtg
      601 tggcatcggt gaaatcctca tccctgaaaa tgagccgggt tcttccatca tgcccggcaa
      661 ggtaaaccct acccagtcag aggcccttac tatggctatg gcccaggtta tgggcaacga
      721 tgtagccatc aacataggcg gcatgagtgg tcactttgag ctcaatgtgt ttaaacccgt
      781 catcatctac aactttctga tgtcggcccg cctcatcgga gacgcctgca tgagttttga
      841 cgaacactgc gccgttggta tcgaagccaa cctgccccgc atcaaacaaa acctcgacaa
      901 cagccttatg ctggtaaccg ctttaaatac caaaatcggt tactacaaag ccgccgagat
      961 cgccaaaaaa gcccacaaag agggcctcac cctcaaacaa gcagcgatgg cgctcggtta
     1021 cgtttccgac aaagacttcg acaaatgggt aaaggccgaa gacatgattt aagtagatca
     1081 aaaatatata tatcaccctg tatttcagca ccttaacaaa aagaaaaaaa tattttctca
     1141 gagtacccaa aaaagatgtt tttttatatc tttgcaccgc tgaaatagca aggattggta
     1201 gttcagttgg ttagaatgcc gccctgtcac ggcggaggtc gcgggttcga gtcccgtcca
     1261 gaccgcaaaa tccccgagga ttttcgaggg ttttttgtct catctctgaa atacttgaca
     1321 ggtaagcctt cttgaaataa ttatcattga tttgactttt taaccacatt gaataagtaa
     1381 aaaattctaa ggtctttaca gaaattcaat gagcaggaga aaaatccggg tagcaagttt
     1441 ttgaaattct atgctgtgtg ggtttctcgt aatgaaatac aaagccttac gattccaaaa
     1501 atcaggatat gcgtgacaat aatagttgct cccgaaggaa gattcaacag gtaggagaaa
     1561 atcaggccaa gtacgtttcc agcgagtcct aaagctatgg ataacagaat catgggtttc
     1621 aatcgtttag tcagcagatt ggccgttaca ggaggaatgg tcatcatagc cagaacgagt
     1681 ataatgccag aaactctgat attaagtaca atagccaggg caatcagcga aatgagcgaa
     1741 taattgatga aattcacagg aatatgatgg gtactggcgt attccatatc aaaggccacg
     1801 gctagtattg ctgggaaaaa ctgtataaaa aacaaaataa taatgatggc gagcacagcc
     1861 atcagccaaa gctcggtatt gcttaccgca agaatgcttc caaacagacc cgacatcaga
     1921 ttgggagcat aaccgggcga aagaaacgta aaaataattc ctatggccat tcccaacgac
     1981 cataacatgc caatggccga atcttctctt acctcagttc gtttggacag atattcaaca
     2041 accaaagcag aaacgagtgc aaaaacagcc gccccaagca gataattaaa accaaagaaa
     2101 taggccaaac ccattccacc aaacgaggca tgtgtgatgc ctccactgat gaaaaccata
     2161 cggcgtgaaa ctatgtaagt tcctataaaa ccacagatga tggccgaaaa gaccgaggct
     2221 aaaagtgcat gttgaaaaaa tacgtaccca aaaggttcaa tgagattcat ttgtagaaga
     2281 attttcctga tgtgtggata gaaccctgtg cggaacaatc ccatgggcaa tcaattcgat
     2341 aggacaattg taaactctca aaatctcggg tgtaatttca ggagttgggt gataatgcag
     2401 cgttccgttt acacaagcta tcgattttat ataggatgga atcattccca gatcatggga
     2461 gaccataaga atagtcatct tttcgttgag ctgaactaat aactcataca gctcggcttc
     2521 gaatcgatta tcggcataag tggaaggctc gtcgagaagt aaaatccaag gttgcgaaac
     2581 gatggctctg gctaaaaata cacgctgcat ctggccacct gataattctc caataggttt
     2641 cgacgccaaa tgacttatct ttaatctttc cagaacttca tgtacagcct ccttatcatt
     2701 tgctctaaaa cggcgcaatc ttcctttagc cgacaataaa cccgtaagaa cgacttcttc
     2761 taccgatatg ggaaacgact tgtcgaaagt attcacttgt ggcagataac ccaatttggg
     2821 tttttctatc gcttgccctt caggactgta aaatatgagc atcccactca aaggttttat
     2881 gatgcctgca attgttttta taaaagtagt tttcccaccc ccatttgggc caattatacc
     2941 aataaaatca ccttcataaa ccgttaggtg aatatgataa atcactggtt tgccataata
     3001 tcctgcgctc aggtcgctaa tttctattaa cttatttctt gtcaaaattt ataggcgttt
     3061 tatttcaatg aattgatttt tcctgaatta atcgtaattg tatcattttt tattagatgg
     3121 gttcaaacct tgataaagca agtcggtcaa tttgatcata ttctgatacc agtcatatgc
     3181 cataggatcg aaaggaactg ctgtaccgcc aatttctttg gctatggttg cagcatttcc
     3241 cgaatcgaac tgactttgat agagtatcat agtgatgccc ttgctgcggg ctaattctac
     3301 gatttccttc attcttctgg tcgaaggaga atttccgcgt tcttcaatag caatttgttg
     3361 gatattaaaa tctctggcca gataagttaa agaaggatga aaaatcatga agctgtgttg
     3421 tgaaacacta tcgagccgct gatgtatgta agcctcca
//
LOCUS       DAORPD010000059         3456 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_4813_length_3456_cov_1.879153, whole genome shotgun sequence.
ACCESSION   DAORPD010000059 DAORPD010000000
VERSION     DAORPD010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4813_length_3456_cov_1.879153"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="PK976_01205"
     CDS             <1..484
                     /locus_tag="PK976_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRC66536.1"
                     /translation="KAYRNEVVKAIEIYGEMHRYIPVIAKWAGFVKIGEKVVQHYPRK
                     HGKSKYGLNRFINGFLDLISISFIGRFGKKPMHFFGLIGTALFFIGFLIACYLAYAKF
                     FLAEYKMTQRPLFYFGLLSMILGTQLFTAGFVGELISRTSHDRNKYLIEKRTGFDRSI
                     "
     gene            468..1607
                     /locus_tag="PK976_01210"
     CDS             468..1607
                     /locus_tag="PK976_01210"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRC66537.1"
                     /translation="MTDQSDIEIFILGTTYPLRGGLATFNERLAQAFKDAGYRVTIYN
                     FSLQYPSLFFPGKTQYSTDPPPVGLNILTKVNSINPFNWIKVGREIKNKRPLLLVLRY
                     WMPFMAPCMGTIARIVKKNHFTKIVAIIDNIIPHEARFFDTCLSKYFLGSCHGFVAMS
                     KSVLEDIGRFGNRKPRKFYPHPLYDTYGKPLSREEACQKLDLDPSFRYMLFFGFVREY
                     KGLDILLQAFTDKRFEKLPVKLIIAGEFYTNAEPYRKMISDLNLSERIVMHNSFISNS
                     EVPAWFCASDIIVQPYKSATQSGVTQIAYYFEKPMIVTNVGGLPEMVPDGKVGYVVEP
                     NPKSLADAIYKFYSLGNTNEFVQHIKKEKQKFTWEGLIRTFEEFL"
     gene            2266..3288
                     /locus_tag="PK976_01215"
     CDS             2266..3288
                     /locus_tag="PK976_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HRC66538.1"
                     /translation="MIYRSKAPLRIGLAGGGTDVSPFCDLYGGAVLNATINLYARASI
                     ICDDSSHIVLHAIDRGFRREYALSEEIIPEKGLELHAGVYNRIVRQFTHQPVAMRLET
                     YVDVPPGSGLGTSSALVVAILGAFTEMLSLPLGEYDMAQLAYEIERQDLRMPGGRQDQ
                     YAATFGGFNFMEFYGDNKVIVNPLRIHQRYLDELTHDLVLFYTQTRRDSSRIIENQAE
                     AIASHHQLRTKATIKLKEQAFMMKEALLKGELRRLGEILNFGWEHKKQIADGITNPQI
                     DSIYSTALQNGAIGGKISGAGGGGFMMFYCPENHRYRLAEALKPFGGFVVNYEFTQQG
                     LSTWSF"
BASE COUNT         1049 a          657 c          668 g         1082 t
ORIGIN      
        1 gaaggcctac cgcaacgagg tggtaaaagc tattgagatt tatggagaaa tgcatcgtta
       61 tattcctgtt attgccaaat gggctgggtt tgtaaagatt ggagaaaaag tcgttcaaca
      121 ctatccccgt aaacatggga aaagtaagta tggactaaat cgatttatca atggatttct
      181 cgatttgatt tctattagtt ttattggaag gtttgggaaa aaacccatgc attttttcgg
      241 tctcataggt acagctctgt ttttcatagg atttcttata gcctgctatc tggcttatgc
      301 taaatttttc ctggcggaat ataaaatgac ccagaggcct ttattctatt tcggacttct
      361 ttcgatgata ttgggaactc aattgtttac agcaggattt gtaggagagc ttatttcccg
      421 aacatcgcac gatagaaata aatatttaat cgaaaaacga accggatttg accgatcaat
      481 ctgatattga aatatttatt ttaggaacca cctatccttt acgcggagga ttagccacct
      541 tcaacgagcg acttgcgcag gcttttaagg acgcaggata tagagttact atctacaatt
      601 ttagtcttca atatccttct ttattctttc cgggcaaaac tcaatattct accgatcctc
      661 cgcctgttgg gttgaacatt ctcactaaag tcaattctat aaatcccttc aactggatta
      721 aagttggacg cgaaatcaaa aacaaacgac ctcttctgtt ggttcttcgt tactggatgc
      781 cttttatggc accttgtatg ggtacgattg cacgaatcgt caaaaaaaat cattttacca
      841 aaatcgtagc catcatcgac aatattattc ctcatgaagc acgttttttc gatacctgct
      901 tatcgaaata ttttttggga agttgccatg gctttgtggc catgtcgaag tcggtgcttg
      961 aagatattgg ccgcttcggc aataggaaac cacgaaaatt ttatcctcat cctttatacg
     1021 atacttatgg caagcccctt tcgcgtgagg aagcctgtca aaagctcgat ctcgatccat
     1081 ctttcaggta tatgttgttt tttggctttg ttcgtgaata caaaggactc gatattcttt
     1141 tacaggcctt caccgacaag cgtttcgaaa aactacccgt aaaactgatc attgctggag
     1201 aattttacac taatgccgag ccttatagaa aaatgataag cgatctgaat cttagtgaac
     1261 gaatcgtgat gcataattct tttatcagca attccgaagt tcctgcctgg ttttgtgctt
     1321 cagacatcat tgtccagcct tacaaatcgg ctactcagag tggtgtaacc cagattgctt
     1381 attatttcga aaaacccatg atagtaacta atgttggagg cttgcccgaa atggtacccg
     1441 atggtaaagt aggctatgta gttgaaccca accctaaatc tttagccgat gctatttaca
     1501 aattctattc cttaggtaat acaaatgagt tcgttcaaca tataaagaaa gaaaaacaaa
     1561 agtttacctg ggagggactc atcagaactt tcgaagaatt tctataacaa ggaaatgaac
     1621 catttttaca ttcaattgat tttcaagcaa ttatcgaaat cttttccgtc ttgaatttcg
     1681 ttatgtattg tggccttttc gctttgtaga tcaattttat cctcgatatt cgcataataa
     1741 ttcgtttcaa aaaaatacca taaaaaattt ccatagaata atttcatggg tttgtaaaat
     1801 tctaaacttt tccttgatga tgatcattga tatcatacaa tctcaccatg atcgtcttct
     1861 atgtcgcctt aatttttctc gacaggtaaa agttgagaat catgatgcag agtatatcat
     1921 ttgccacagc cgttatcatg catgttaaaa ccttttaatt tatccaaggt gtacattctg
     1981 gcacaaacaa cttatacttg cgaacacaac cgtgacaaac ctcggaagcc agatgtaaca
     2041 attacatcag catgcacttc tgcaaggagt atattctcga aaaaatcgat cgcttgctca
     2101 ataaataaat gttgaggcaa taaaattgag tttccgacgc atgcaatgga actaaagtca
     2161 gtcagccctt cattaccgaa aaggtcgtaa ttttacattc tcatttcaac gaatgaaagt
     2221 tacacgaaat atcttcaaaa aatatatttg acaaaaccta ggcaaatgat atacagaagt
     2281 aaagctccat tacgaattgg ccttgcagga ggaggaaccg atgtatctcc tttttgtgat
     2341 ctatatggag gagcggtgct caatgctacc atcaatcttt atgcaagagc ttccattatt
     2401 tgcgatgatt catcgcacat tgtcttacat gccattgaca gaggttttcg cagagagtat
     2461 gctctatcag aagaaattat tcccgaaaag gggctcgaac ttcatgcagg agtttataac
     2521 cgaatagtta gacagtttac gcatcaacct gttgctatgc gattagaaac ttacgtcgat
     2581 gtgccgccag gttctgggtt gggaacttcg tcagcactcg tagtagccat actcggagct
     2641 tttactgaga tgctttcatt gcctttagga gaatatgata tggctcaatt ggcttatgaa
     2701 atcgaaagac aggatttaag aatgcccggt ggccgtcaag accaatatgc agctactttc
     2761 ggtggtttta actttatgga attttatggt gataataaag tcatagttaa tccacttcgt
     2821 attcatcaaa gatatttaga tgaacttacg cacgatttgg tgctatttta tacacaaacc
     2881 cgtcgcgatt cgagccgtat catcgaaaat caggcagaag ccattgccag tcatcatcag
     2941 cttcgtacca aggccactat aaaattgaag gaacaggctt ttatgatgaa agaagctcta
     3001 ttgaagggcg aattgagaag attaggcgaa atcttgaatt ttggctggga acataagaaa
     3061 caaatcgctg atggaattac caatccacaa attgatagta tctatagcac tgctttacaa
     3121 aatggagcta ttggaggtaa aatttcagga gccggtggtg gtggattcat gatgttctat
     3181 tgtcccgaaa atcatagata tcgattggct gaagctctta aacctttcgg aggttttgtc
     3241 gtgaattacg aattcaccca acagggtttg tctacttggt cgttttaatt aaatatttta
     3301 cttgaagtat ggaaaagttt attatttcaa gaatcgaaga atctatcgtc ctgaaacaga
     3361 atatcatcaa caatagagag cttttgcaaa atcttgctct tttgacagaa gcctgtattc
     3421 gatgtttcaa ggaaggaggc aaggtacttt tttgcg
//
LOCUS       DAORPD010000060         3390 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_4926_length_3390_cov_2.561319, whole genome shotgun sequence.
ACCESSION   DAORPD010000060 DAORPD010000000
VERSION     DAORPD010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4926_length_3390_cov_2.561319"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..1331
                     /locus_tag="PK976_01220"
     CDS             159..1331
                     /locus_tag="PK976_01220"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC66539.1"
                     /translation="MKYLTRTIWILSLVSLFTDIASEMLYPVMPIYLKSIGFTVVLIG
                     VLEGLAEATAGLSKGYFGKLSDEKGKRVPFIRIGYGLSAISKPLMAVFIYPLWVFFAR
                     TLDRLGKGIRTGARDALLSDESTPETKGRIFGFHRSLDTVGAAIGPLFALLFLYFYPN
                     QYKTLFFIAFIPGLLAIVLTFLIKEKPSIQKSSHNKVSFFSFINYVKTSNNTYKKIIA
                     GLLFFTLFNSSDVFLLLKIKEAGLNDTWVIGTYIFYNMVYALASYPLGIIADRLGLKK
                     IFVFGLLMFSIVYFGFAFNTKFTIFLGLFVVYGIYAAATEGISKAWITNVSPKGETAT
                     AIGAYTAFQSVFTMLASSLAGLLWYNFGATVALLTTAITSLITVIYFIFVVKPKTV"
     gene            1397..2182
                     /locus_tag="PK976_01225"
     CDS             1397..2182
                     /locus_tag="PK976_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66540.1"
                     /translation="MISSSNSIDAHTDEKFAPDSLQTLLVRILSGSDTIDDSILYFPR
                     SIELRLITNNEKAEIFYSLKNTINEIGFLQYHQPLLFNSSTTLLAYAILPSHIKSTMK
                     ELRLVKIENLFDFQIFCDERRCCQSSRRYKLFDGVTDNSETAENGNDYLCLKGSKIEF
                     WVDFGIKRQVNEVKFHLYVAETKNLILPSILVEGSENGYEFYPLGQKRFSVTFNKSFW
                     VKVELQPAKVRYIRIGFYNSSQSIENDCILIDEIHFDSKEEEF"
     gene            complement(2277..2927)
                     /locus_tag="PK976_01230"
     CDS             complement(2277..2927)
                     /locus_tag="PK976_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013263964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC66541.1"
                     /translation="MMKVENIHKRYGTVEVLKGIHLEVYSKEILAIVGSSGAGKSTLL
                     HIMGTIEKPDQGKVFFKQMDLFKMKEKQLSAFRNQQLGFVFQFHHLLFEFTAIENVCI
                     PALIAGKDKNAIKKKAEEWLAFFGLAHRKDHKPTELSGGEQQRVAVARALINDPPLIL
                     ADEPSGNLDSKNAEELHRLFFELRNQYEKTFVIVTHNPSLSDLCDRRLLIKDGILQ"
     gene            complement(3096..>3390)
                     /locus_tag="PK976_01235"
     CDS             complement(3096..>3390)
                     /locus_tag="PK976_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66542.1"
                     /translation="RKGGYKGSTYGIDIIGKLYPYHNFYLEANYSNLNTGLSSIGVVK
                     DRIWTKALLIGGGYSQQLSEKVFLNFSILWDVIGEEWYPFDNPLIRAGIVTRL"
BASE COUNT          994 a          613 c          606 g         1177 t
ORIGIN      
        1 taactaactg aataagtggt atataaaaat gtttaggaac tttttagaat tttgtcggtt
       61 aatagtgatt atttatatat ggcataaatt accgcttctt tgaaagttgc tatcaagatt
      121 tttctatatt tgcaggtttg aggaatgaaa atttctttat gaaatatcta actcgtacca
      181 tctggatatt atcgcttgta agtttattta ctgacattgc aagtgaaatg ctttatccag
      241 ttatgccgat ttatttaaaa agcataggat ttacagtagt tttaattggc gtattggaag
      301 gtttggccga agcaaccgcc ggtttgagca agggttattt cggtaaactt tccgatgaaa
      361 aagggaaaag agttccattt atcaggattg gatacggcct gagtgcgata tctaagcctt
      421 tgatggcggt ttttatatat cccttatggg tgttctttgc ccggacactc gaccgcttag
      481 gaaagggcat acgtaccgga gcacgcgatg cattgctttc cgacgaatcc acacccgaaa
      541 ccaaaggtag gatttttggg tttcatcgtt ctttagatac cgtgggagcc gctattggtc
      601 ctttgtttgc tctccttttt ctttattttt atcccaatca atataaaacc ctctttttta
      661 ttgcttttat tccaggttta ctggctattg ttcttacttt cctcatcaaa gaaaaacctt
      721 ctattcaaaa atcctcccat aataaagttt cttttttctc attcatcaac tatgtgaaaa
      781 cgagcaataa tacttataaa aaaattatag ctggtttatt gttctttacg cttttcaaca
      841 gttccgacgt ttttttatta ctcaaaataa aggaagcagg acttaacgat acctgggtga
      901 ttggaaccta tattttctac aacatggttt atgcattggc ttcatatccc ttgggaataa
      961 ttgccgacag attggggctg aagaaaatat tcgtttttgg tctcctaatg ttctccatcg
     1021 tttattttgg ctttgccttc aatacaaaat ttactatttt tttgggatta tttgtggttt
     1081 atggtattta tgctgctgct accgagggaa ttagcaaagc atggattacc aatgtttctc
     1141 caaaaggaga aacagctacg gccattggag cttatacagc ttttcagagc gtttttacca
     1201 tgctggcgag ttcattggct ggcttgcttt ggtataactt tggcgctacc gttgctttgc
     1261 ttactaccgc aataacttcc ctgattacag taatatattt tattttcgtg gtaaaaccaa
     1321 aaacagtttg atgatcctac acaataaaac ttcttgctta cttacaatta ttcaattttt
     1381 aacaatttac gcattggtga tttcgtcatc caattctatc gatgcccata ctgacgaaaa
     1441 atttgcacct gatagcctac aaactttatt agtccgaatt ttatcgggaa gcgataccat
     1501 tgacgattcc attctgtatt ttccccgatc gatagaatta agactgataa ccaacaacga
     1561 aaaagcagaa attttttatt cgttgaagaa tacgataaat gaaataggat tccttcaata
     1621 ccatcaaccg ttgttgttca attcttctac tacacttctg gcttatgcta tattaccatc
     1681 ccatataaaa agtactatga aggaattgag gcttgtaaaa attgaaaact tatttgattt
     1741 tcagattttt tgcgacgaga ggagatgttg ccagagttca cgacgttaca aattgttcga
     1801 tggcgtaacc gacaattcag aaactgcaga aaatggaaat gattacctgt gtttaaaagg
     1861 atcaaaaatt gagttttggg tggattttgg gataaaacga caagtaaatg aagtaaaatt
     1921 ccatctttat gtggcagaaa cgaagaacct tatcttacct tcaatattgg tagaaggttc
     1981 agaaaatggt tatgaatttt atcctctggg acaaaagaga ttttctgtta cttttaataa
     2041 atctttttgg gtgaaagtgg aattacagcc tgcaaaagtt cgttatatca gaattggttt
     2101 ttataattca tcacaaagta ttgaaaatga ttgtattttg atagatgaaa ttcatttcga
     2161 ttcgaaagaa gaagaatttt aaggtatcgg tataatgttc attttcacct agtacagttt
     2221 gcatcagata agagacaatg ttttcgagaa gaagaaaatg gaaatttcac gaaaaatcat
     2281 tgtaatattc catccttgat taacagacgc ctgtcacata gatcggaaag agaaggattg
     2341 tgagttacaa tgacgaaggt tttttcgtat tggtttctta attcgaaaaa cagccgatgg
     2401 agttcttcgg catttttgct gtcgaggttt cccgagggtt catcggccag aataagggga
     2461 ggatcattga ttaaagcgcg tgctacggca acacgttgtt gttctccacc cgaaagttca
     2521 gtgggcttat ggtctttgcg gtgggctaat ccgaaaaaag ccaaccattc ttcggctttt
     2581 ttctttatgg cgtttttatc ttttcctgca ataagagctg gaatacatac attttcgata
     2641 gcggtgaact cgaaaagcaa atgatgaaat tgaaaaacga atcctaattg ctgattacga
     2701 aaggccgata attgcttttc cttcatcttg aataaatcca tttgtttgaa gaaaactttt
     2761 ccctggtctg gtttttcgat tgttcccata atatgcagta aggtagattt cccagcaccc
     2821 gaggacccta cgattgctag aatttctttg gagtatacct ccagatgaat tcctttcaac
     2881 acttccactg ttccatatcg tttgtggata ttttccactt tcatcatcgt ttccatttta
     2941 taaactttaa tgctttattg ccttcttgcc atgttttata tttgtatttg cactttgagt
     3001 cgtcaacttg cggatctgat caacttttct ataaatctat aaaatttacc tatcgtaata
     3061 cagatttccc ctttattgtt tatcattaca atgacttata atcgagttac aatacccgcc
     3121 ctaatcaaag ggttatcgaa tggataccat tcttctccta taacgtccca caagatacta
     3181 aaattaagaa aaactttttc actcaattgc tgactataac ctccaccgat caataaagcc
     3241 ttggtccata tcctgtcctt caccactcct atcgaggata aacccgtgtt taaattggaa
     3301 taatttgctt cgagataaaa attgtgatag ggatacagtt tcccaataat atctattcca
     3361 taagtacttc ctttatagcc gccttttctt
//
LOCUS       DAORPD010000061         3390 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_4927_length_3390_cov_2.164018, whole genome shotgun sequence.
ACCESSION   DAORPD010000061 DAORPD010000000
VERSION     DAORPD010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4927_length_3390_cov_2.164018"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3141)
                     /locus_tag="PK976_01240"
     CDS             complement(<1..3141)
                     /locus_tag="PK976_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRC66543.1"
                     /translation="MYKITTHPILEVPKAEKVTFRYDGHIIEAEKGYTIAAALHQAGF
                     PVHSHSLRNRKRSLECGIGKCGACEMLVNGQVKRICITKVDGVHEVKEIPHDYRSGVV
                     EYRKNEPIDVYKTQVAIIGAGPAGLAAREILCQFGIDNLVVDNNSKIGGQFLMQTHQF
                     FFFEKEKKFGGMRGFDIAQTLAGTDHDGIFLNSTVWDILEGGRLAVKEITTDRIYYID
                     AEYLVVSTGAVPFMPSFENDDLPGVYTAAVVQKMMNMQFTLLGKNVLTVGAGNIGYLT
                     SYQLMQAGARVKVILEAQPFEGGFPVQANRVRRLGIPVLTSHILLKAIPNRDLTGITG
                     AVIALCKNFHPVVGTETVVEGIDAINICTGLVSDDQLLIKGNEVFGRKCYGAGDTIRI
                     GEGTSAVLRGKQVAYEILMEMGIRLNYDDYLSLSKEYIDSQQHPIRIIDRPYMPDHER
                     MQAKGFVLIDCLYGFACNPCAFACPQGAITKSSTSTVPSIDFDKCIGCMDCVYQCPGL
                     AIFGYNLKRDWVFLPIEYDVKENAEVFLVDNYGKILGEGVIDKILRKPNKTNIARVKS
                     LSLHGEDLTQVRSFIVKKNYPVPLELRPYDEQIPVESYICHCDDVKIEEILEVVGERK
                     FISIDEIKHTTRLGMGACRGKRCIPRLKSKIKDYGIQIVGEATPRGPMSNQLSLGELY
                     PRPVHENYVVNRSVRKEKVETLIAGGGMGGSALFRYLSEAGKKPVLINFGRGSSWRNI
                     AGGRPNFSVPELSDIAEHNRQIFIELQQLHNIDYHPIQYVNFAHDEEMYKALEAAMAW
                     SKAEMISPKDFTKRISPFFNPGLKTYIAAQITYNCWQATPGKTIDLIRRIGIQQGGTI
                     LEDCKLIDVRKNGQEYQVLVQDHTKQYIEYSSEIFINALGPEAAYFARLLGIETGLYP
                     VRHQAFITRRMPMLGVNDIPLPMLIDRRKYKGFVAVYGQQLAETGQVIGCASPAVDPA
                     ETLKNLKVNSREFLEIFSEVFVDWLPELSSVGFQAVWSGYYVEPRMIIDPDLGLFVGL
                     RGQGFMLSQYLAKMYVD"
BASE COUNT          978 a          835 c          614 g          963 t
ORIGIN      
        1 atcgacatac atcttggcaa ggtactgact tagcatgaaa ccttggccgc ggagcccgac
       61 aaataatcct aaatccgggt ctatgatcat tcgtggttcg acataataac ctgaccagac
      121 cgcctgaaaa cccaccgagg ataattcggg caaccaatcc acgaaaactt ctgagaaaat
      181 ttcgagaaat tctctcgagt taactttcaa attcttgagg gtttcggcgg gatctactgc
      241 cggagaagca cagcctatga cctgacccgt ttcggctagc tgctgcccat aaacggccac
      301 gaaacccttg tatttccttc tgtcgatcaa catcggaagt ggtatatcgt tgacaccaag
      361 cataggcatt cgacgcgtaa taaatgcctg atggcgtacg ggatagagac ccgtttctat
      421 tcctaataat ctggcaaagt atgcggcttc tggccctaat gcattgatga agatttcact
      481 actgtattca atgtattgtt tggtatgatc ttgaaccaaa acttgatatt cctgaccatt
      541 ctttcttaca tcgattaact tgcaatcttc caaaatcgta cctccttgct gaattccaat
      601 ccgacggatt aaatcgatgg ttttgcccgg agtagcctgc caacaattat aagtaatctg
      661 tgcagcaata taggttttta aacctgggtt gaaaaatgga gaaatccgtt tcgtaaaatc
      721 cttcggactg atcatttcgg ctttgctcca tgccatggca gcttccagtg ccttgtacat
      781 ttcttcatcg tgtgcaaaat tcacgtattg tatgggatga tagtcgatgt tgtgcagttg
      841 ttgcaattcg ataaatatct ggcggttatg ctcggcaata tcggaaagct caggaacact
      901 gaaattggga cggcctcctg caatattcct ccatgaagaa ccacggccaa aattaatcaa
      961 taccggcttc ttgcctgctt cggataaata tcgaaataag gcacttcctc ccatgcctcc
     1021 acctgctatt agagtttcca ctttctcctt tcttacagaa cggtttacta cgtaattttc
     1081 atgaactggc ctgggataaa gctctccaag cgaaagttga ttcgacatcg gtccgcgggg
     1141 cgtggcttcg cctacaatct gaattccata atcctttatc ttggacttca atcggggtat
     1201 gcaacgtttt ccccgacatg cacccatccc taaacgtgta gtgtgcttta tctcatcaat
     1261 ggatatgaat ttcctctcac ctaccacttc taaaatctcc tcaattttta catcatcgca
     1321 atgacagata taactttcaa ccggaatctg ttcatcataa ggccgtagct ctaaaggcac
     1381 tggataattc ttcttgacta tgaagctacg cacttgtgtt aaatcttctc catgcaggct
     1441 gagcgacttt actcgagcaa tattggtttt attgggtttc cgaagaatct tatcgattac
     1501 cccttctcct aaaatttttc cataattgtc aacgagaaaa acttcggcat tttcctttac
     1561 atcatattct atgggcaaaa atacccaatc gcgtttcaaa ttataaccaa aaatagctaa
     1621 gcccggacat tgataaacac aatccataca gccaatgcac ttgtcgaaat cgatggaagg
     1681 aactgtactg gtcgaagatt ttgtaatggc accctgagga caggcaaaag cacaaggatt
     1741 acaggcgaat ccataaagac aatcgatcaa cacgaatcct ttggcttgca tcctttcatg
     1801 gtcgggcatg taaggtcgat cgatgattct tattggatgt tgctgcgaat cgatatattc
     1861 tttcgaaagt gaaagataat cgtcataatt caaacgaatt cccatttcca taagaatttc
     1921 ataggctact tgcttccctc tcagaaccgc actcgtgcct tctccaatgc gaatggtatc
     1981 gccagcacca tagcattttc gtccaaaaac ctcgttcccc ttgatcagta gctgatcatc
     2041 cgaaaccaat cccgtacaaa tattgatggc atcgatacct tcgactacag tctcagttcc
     2101 aacaacagga tgaaaatttt tgcaaagtgc tattacagca cccgtgattc ccgtcaaatc
     2161 tcgattgggt atagctttga gcagaatatg cgaggtcagt acaggaattc ccaatcttcg
     2221 cactctattc gcctgaacgg gaaatccgcc ttcaaagggt tgagcctcca aaatgacttt
     2281 tacccttgct cccgcctgca taagctgata agaagtcaaa tatccaatat tccccgctcc
     2341 aaccgtcaaa acatttttac ccaagagggt aaactgcata ttcatcatct tttgaactac
     2401 cgcagccgtg taaactccgg gtaagtcatc attctcaaag gaaggcataa atggaacagc
     2461 cccggtagat acaaccaaat attctgcatc gatataatag attctgtcgg tcgtaatttc
     2521 cttcacggca agccgacctc cttccagaat atcccatacc gtcgaattca aaaatatacc
     2581 atcatggtct gtcccagcta gggtttgagc aatatcgaaa cctcgcatcc ctccaaattt
     2641 cttttccttc tcaaaaaaga aaaactgatg ggtttgcatc agaaattgcc caccaatctt
     2701 actgttgtta tcaactacca aattatcgat cccaaactga cataagattt cacgggcagc
     2761 caaacctgcc ggcccggcac caataattgc gacctgggtt ttataaacat cgatgggttc
     2821 attcttgcga tattcgacaa cccccgacct gtaatcatga gggatttctt ttacctcatg
     2881 cactccatcg accttggtaa tgcaaatgcg ctttacctgc ccattcacca gcatttcaca
     2941 agcaccgcat ttccctatgc cacattccaa actcctctta cgattgcgta aactgtgact
     3001 gtgtacagga aatccagcct gatgaagcgc tgcagctatg gtataaccct tctcagcttc
     3061 gatgatgtgc ccatcgtatc taaaagtaac cttttctgcc ttgggaacct caagaatggg
     3121 atgtgtggta attttataca tgatatttcc tttaatgtga ataaaaaaac attgtgaaaa
     3181 atttcacagc aaaattactg ggtttatttc tgaaccacac cccataaaaa cggaaaatct
     3241 cctattcata atcatttcaa ataacaaccc aaaccattga taaactttgc tacattcaaa
     3301 ccatcggcct tcatccagtc agaatttctt gacaaaacaa tatttcgaaa ggtttgcttc
     3361 ggaaaaacca tgtcaaaatg gaaaggttcg
//
LOCUS       DAORPD010000062         3388 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_4931_length_3388_cov_2.459946, whole genome shotgun sequence.
ACCESSION   DAORPD010000062 DAORPD010000000
VERSION     DAORPD010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4931_length_3388_cov_2.459946"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(153..1541)
                     /locus_tag="PK976_01245"
     CDS             complement(153..1541)
                     /locus_tag="PK976_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRC66544.1"
                     /translation="MNTKKDMLPQTSDTKSSKKSGSKKIFVLDTSVILYNHASFYSFD
                     DNDIAIPITVLEELDNFKKGNEIKNFEAREFIRIIDRLSHKKNLTDWVAIDNGKGGSF
                     RVILDQPHEQKVQQIFGGYKNDHRILNAALTLKEIYPERKVILVSKDINLRIKAKALN
                     LQAEDFETGKIKDIENLDPGKKVISHIAPQVLSKLWTTGKCSPSEIGEKEPIPNYYYI
                     FQNASENENPTLAFYNPKSGNIERIDPQPVERIMPRNPEQAIALHAITNPNVKLVTLI
                     GIAGTGKTLLALAGAPEQRRLYKQIYLARPIVPLSNKDIGFLPGSAEEKISPYMEPLY
                     DNLKFIKNQYSEKEREYKLFDEMLENGKIVIAPLAFIRGRSLSNICFIVDEAQNLTPH
                     EVKTIITRAGEGTKIIFTGDIYQIDTPYLDSQSNGLSYLVDKIHSHPIYAHVKLERGE
                     RSELANLASELL"
     gene            1970..2761
                     /locus_tag="PK976_01250"
     CDS             1970..2761
                     /locus_tag="PK976_01250"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HRC66545.1"
                     /translation="MKKSFNAYGGIFIQVLLLSMMMISCVPQKKIKYFQSLAKEDTLE
                     IFEFSPKPDYRLKVGDNLYIKVQSLDEKTYKFFNYEDMGRATNIGYSDANIYLTSYTV
                     SDSGYIHFPFVGKIFVSGLNVDEVSKKLQDIVDQYLVETTVIVKLVNFKITVLGEVKN
                     PGTYLIYQDKINIYEALAKAGDLTVFSDRNKVVLVRQDQNGAELHRIDLTKKNFLESP
                     YLYLLPNDIVYVEPLRAKQYAFAAFPYDLLFSTITTTLLILNYFK"
     gene            2782..>3388
                     /locus_tag="PK976_01255"
     CDS             2782..>3388
                     /locus_tag="PK976_01255"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HRC66546.1"
                     /translation="MDNNVNMPDYQQQEENIDFKQIFFKFLRYWYLFALAIFVALVIA
                     FLFNKYTTPTYEVSTTVLIQDKGNKEINAQALMGLGFMNSQQNIQNELGILSSYTLTS
                     EAVKVLNVDVTYYSENNFIVRELYRKSPFTVFYDSIHPQPVHLRFYVTILNNRQFKLE
                     TQGENIKLFDFENNKFIEEQQDGETKIKTIKLDQIYDFGTTI"
BASE COUNT         1006 a          639 c          667 g         1076 t
ORIGIN      
        1 catctctttt cgccgattgt ctcagtttgg gcgtatcgaa acaactgcca ttggagattt
       61 ttctactgga tgatttgggt tgacaacctg atccattaga atagaccggt tcaagcgcag
      121 cataagccga aaggggtgcc atcaaaaaaa gttcaaagta attctgaggc aagatttgcc
      181 agctccgaac gctcacctct ttcgagtttt acgtgagcat agatgggatg gctgtggatc
      241 ttatccacca ggtacgacaa gccattcgac tgtgaatcaa gataaggagt atcgatttgg
      301 taaatatcgc ctgtaaagat gatttttgtg ccttcgcccg cccgggtgat aatggttttt
      361 acttcgtgag gcgtgagatt ttgagcttca tccactatga aacaaatatt tgaaaggctt
      421 ctcccacgaa taaaagctaa aggagcaatg actatctttc cgttttcgag catctcatcg
      481 aaaagtttat attctctttc cttttcggaa tattgatttt tgatgaattt tagattgtcg
      541 tataaaggtt ccatgtaagg actgattttt tcttcggcgc tgcccggaag aaaaccaata
      601 tccttattgc tcaggggtac gataggcctt gctagataaa tttgcttgta aagcctccgt
      661 tgttcggggg cacctgccag agccagcaga gttttacctg ttccggctat tcctatcaaa
      721 gtaaccaact ttacattggg atttgtaatg gcatgaagtg caatggcctg ttcggggtta
      781 cgaggcatga tgcgctctac aggctgagga tcgatgcgct ctatgtttcc gcttttcggg
      841 ttataaaatg ccagcgttgg attttcgttc tcacttgcat tctgaaaaat ataataataa
      901 tttgggatag gctctttttc accaatttcg gagggagaac acttccccgt agtccagagt
      961 ttcgatagaa cttgcggagc tatatgggat attaccttct tgcccggatc gagattctca
     1021 atatccttga ttttccccgt ttcgaagtct tcagcctgaa gattgagagc tttggctttt
     1081 attctcaaat tgatgtcttt cgaaactaaa atgactttgc gctctggata aatttctttt
     1141 agtgtaagag cggcatttaa aatacgatgg tcatttttat atccaccaaa aatttgctga
     1201 accttttgtt cgtgaggttg atccaaaatt actctgaaac ttccaccttt gccattgtcg
     1261 attgcaaccc aatcggtaag attcttctta tgcgataatc tatcgatgat gcggatgaat
     1321 tctctggctt cgaaattctt gatttcgttc cctttcttga aattatcgag ttcttccaat
     1381 acggtgatgg gtatggcaat gtcgttatca tcgaaagaat agaaagaagc atgattgtat
     1441 agaatcaccg aggtgtcgag aacgaatatt ttctttgatc cggatttttt cgaagattta
     1501 gtatcagaag tttggggaag catatctttc tttgtattca tagcctgtgg aattttatag
     1561 gttttttatg cttgcgaaga tacatgttct ttacgtggtg tcgatgcctt aagtaggatg
     1621 ttcgattctt cctataacta gaaatggcaa atctatggtg ctcttgaaat cttcatacac
     1681 gagattgatg gtgctgctat ttgcatgata gggcttgtcg aattttgagt gaatgttgaa
     1741 agaatctgaa atttttcgat tgaaggagaa aattatgcat ggtatgggtt gtttcatgaa
     1801 aaaaataatt tatttttgcg ccagaaaata aggtcatcca aaaagacact aaagggccat
     1861 atagttgaac aggttaattg aaatccttac aataaaaact ctcctatcgg gtccgatcaa
     1921 cagcaatggc ttacaagatg tcttatcata aatgcataaa cactcaggaa tgaaaaaaag
     1981 tttcaacgct tacggaggta tttttattca ggttcttcta ttatcgatga tgatgatttc
     2041 gtgtgtacct cagaaaaaaa tcaaatattt tcaatcactt gccaaggaag atacactcga
     2101 gattttcgaa ttttccccaa aacccgatta tcgtttgaag gtgggcgata acctttacat
     2161 caaagttcaa agcctggacg aaaaaaccta taagttcttc aattatgaag acatgggtag
     2221 agcgaccaat ataggttata gtgatgcaaa catatatctt acaagctata ccgttagcga
     2281 ttcgggctac attcattttc ctttcgtggg caaaattttt gtctcggggc tcaatgtaga
     2341 tgaggtgtcg aaaaaacttc aggacattgt cgaccaatac ctggttgaaa ccactgtaat
     2401 cgtaaaactg gtcaatttca agattaccgt attgggagag gtaaaaaatc ccgggactta
     2461 tcttatttat caggataaaa tcaatattta tgaggcattg gctaaagctg gcgatcttac
     2521 cgtatttagc gaccgaaata aagtggtgct ggtaaggcaa gaccaaaacg gtgccgaatt
     2581 gcatcgtatt gatttgacta aaaagaattt tctcgaatcg ccttaccttt atttacttcc
     2641 taatgacatt gtctatgttg agcctttaag agctaaacaa tacgcttttg ctgccttccc
     2701 gtacgacctt cttttttcta ctatcacaac aacattgctc attctcaatt atttcaaata
     2761 gaatcttaaa agtatcgaaa agtggataac aacgtcaata tgcccgatta tcagcaacaa
     2821 gaagaaaata tcgatttcaa acagattttt ttcaagttct tgagatactg gtatctgttc
     2881 gcactcgcca tatttgttgc tttggtgatt gcctttttgt tcaacaaata caccaccccc
     2941 acctatgagg tttctaccac agtattgatt caggataagg gcaataagga aataaatgcc
     3001 caagcactga tgggcctcgg ttttatgaat tcccagcaaa atattcaaaa tgaactggga
     3061 attctcagta gctacacact tacatccgaa gccgtgaaag tattaaatgt tgatgtaacc
     3121 tattattccg aaaacaactt catcgtaaga gaactctata gaaaatctcc ttttacagta
     3181 ttctatgatt ctatccatcc acaacccgta catctgagat tttatgttac tatacttaat
     3241 aatagacaat ttaaactcga aacccagggt gaaaacatca aactcttcga tttcgagaat
     3301 aataaattta ttgaggaaca acaagacgga gagactaaaa taaaaaccat caaactggac
     3361 cagatttatg attttggaac taccattt
//
LOCUS       DAORPD010000063         3377 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_4955_length_3377_cov_1.998495, whole genome shotgun sequence.
ACCESSION   DAORPD010000063 DAORPD010000000
VERSION     DAORPD010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4955_length_3377_cov_1.998495"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2080
                     /locus_tag="PK976_01260"
     CDS             <1..2080
                     /locus_tag="PK976_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HRC66547.1"
                     /translation="DPKDALGLLSYLNREQYGNWPLVYGADFTADIVDYKDGEPVYMK
                     DYTAGRYVAKDSRKAAKPVYNSRMFFPRMYSRSYKHIQEYKIWAGIPDDENYRPTFGD
                     HLGFLINYQLNQMYWRYFLWNFSGRQNDSQGFSDKANGNWISGWNFLDKWRVGPLSHL
                     PEHKKSRAWNRYYLLPLLFGLVGMVYHYKNDWKSLMVVLVFFIMTGIAIIVYLNQYSP
                     QPRERDYAYVASFCAFAIWIGMGTGAFASGMTKWINGRKSILLTTGLNLLCVTGVLAA
                     QGWNDHNRSNRYATTQMAKAYLDSCASNAILFTFGDNDTFPLWYLQEVENYRTDMRVC
                     NLSLLSLDWYIEQMKRKVYESAPLPIQLDFSFYKQGTHDYIYFISDDDSLTDTLNLCS
                     IFEQMSVEPQKFKYVIETDTIDYLPSNRFVLNIDKTAVLNHGVIDSDQKDRIVDRMFF
                     EIPGREFEKNTLIVLNILANNNWGRPVYFGLTGNTREFLGLEEYFRLEGMAYRLVPIL
                     MKSRHDRMGGIDSEILYDHLMNRSQVKMNNPEIYYSENHLKNCTLLRNIFRSLADTLL
                     KEGKKELAVRVCDRCIVNLPNSVIPYDVFSLALAEVYYGAGEISKGNEIMTVMMNQSS
                     AELGYYFELLPNLLHRYEYEINLNLGILNYIAETACKFGQEEIGDKSKEAFEKNLKCY
                     FTQIYQNFEP"
     gene            2086..2715
                     /locus_tag="PK976_01265"
     CDS             2086..2715
                     /locus_tag="PK976_01265"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRC66548.1"
                     /translation="MIKATTCFPRAVEWIFAPFVITRMPRTGQCLYLTFDDGPHSQIT
                     PKVLDILNRFQVKATFFCLGKNIRAYPEVFQEIKNNGHNIGNHSFSHKNGWKTANKEC
                     FKDIEACRELIPTNLFRPPYGRMRVSQVRVLSRQYQIILWTLLSGDYNRSLSSEEILR
                     TLLRRTRSGDIIVFHDNEKAFPCLIEVLPSYLEAMLEKGFTFDVIQSKL"
     gene            2718..>3377
                     /locus_tag="PK976_01270"
     CDS             2718..>3377
                     /locus_tag="PK976_01270"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRC66549.1"
                     /translation="MESRRYFIRILKKAIDNAMLALGLGLLLLMCRCANPVVPQGGPK
                     DTDPPKLLYAVPPNLSTHFTASHIKLTFDEYVVLKEINKKFLMSPPSLNLPEFKVKGK
                     SLEIDLKDTLRPNTTYSLYFADAITDLHENNPLPDFQYVFSTGAVIDSLTMTGSVVNA
                     FNLKPMENMVVMLYYANNDTLPLEQLPLKVPPSYAARTRKDGSFNLNYLRDEKYLLFA
                     LI"
BASE COUNT          952 a          654 c          747 g         1024 t
ORIGIN      
        1 tgatcctaag gatgccttag ggcttttgtc ataccttaac cgggagcaat atggcaattg
       61 gcctttggtt tacggagccg atttcacggc agatattgtc gattataagg atggtgagcc
      121 agtttacatg aaagattaca cggctggacg ttatgttgcg aaagatagca ggaaggctgc
      181 gaaaccggtt tataattctc gaatgttttt tccaagaatg tattctcgtt cgtacaaaca
      241 tattcaggaa tataagattt gggctggtat tcccgatgac gaaaattaca ggcctacttt
      301 tggagatcat ttgggctttc tgataaatta tcaactgaac caaatgtact ggagatattt
      361 tttatggaat ttttctggtc gccagaatga ttctcaagga ttctccgata aagccaatgg
      421 aaactggatt tcggggtgga atttcttgga taaatggaga gttggccctt tgagtcacct
      481 gcccgaacat aaaaaaagca gggcatggaa tcggtattat cttttgcctt tgttgtttgg
      541 tctggttggt atggtatatc attataagaa cgattggaaa agcttgatgg tggttttggt
      601 gtttttcatt atgacgggaa ttgccatcat cgtttatcta aatcaataca gtccgcagcc
      661 ccgtgaaaga gattatgcct atgtggcttc gttctgcgcc tttgccatct ggataggaat
      721 gggcacgggt gcgtttgcct caggtatgac aaaatggata aatggcagaa agtcgattct
      781 tctgaccaca ggtttgaatc ttctttgtgt aaccggtgtg ttggccgccc aggggtggaa
      841 cgaccataac cgctcgaacc gatatgccac tacacagatg gctaaagcct atctggatag
      901 ctgcgcatcc aatgccattt tgtttacttt tggcgacaac gatacctttc ctctttggta
      961 ccttcaggag gtagaaaatt accgtaccga tatgagagtg tgtaacttga gtcttttgag
     1021 cctcgattgg tacatcgaac agatgaaacg caaagtttat gaatcagcac ccctgcccat
     1081 tcaacttgat ttttcttttt ataagcaagg aacacacgac tatatttatt ttatttcgga
     1141 tgatgattcg ctgaccgata cgctaaatct gtgcagtata ttcgagcaaa tgtcggtgga
     1201 acctcaaaaa ttcaaatatg tcatcgaaac cgatacaata gattatttac catcgaatcg
     1261 atttgtattg aatatcgaca agactgcagt gttgaatcat ggtgttatcg actctgatca
     1321 gaaggataga atcgtagatc gaatgttttt cgaaataccg ggcagggaat tcgaaaaaaa
     1381 taccttaatt gttttgaata tcttagcgaa taataactgg ggaaggccgg tatattttgg
     1441 tttgacggga aacacccgcg agtttttagg acttgaagag tattttcgtc tcgaaggcat
     1501 ggcctatcgc ctggttccta ttttgatgaa atcaaggcat gatcgtatgg gagggattga
     1561 ttcggagatt ctttacgatc atttgatgaa tcgttcgcag gtgaaaatga ataatcctga
     1621 gatatattat tcggaaaatc atttgaaaaa ttgcactttg ctacgcaata tattcagaag
     1681 tctggccgat accttgttga aggaagggaa aaaggaattg gcggttagag tatgcgatcg
     1741 atgtatcgta aaccttccga attccgttat tccttatgat gttttttctt tggcgcttgc
     1801 cgaagtgtat tacggggcag gtgagatttc gaaaggcaac gaaataatga ccgtgatgat
     1861 gaatcaatcc tcggccgagc tcggctatta tttcgagttg ttgcccaatt tgcttcatcg
     1921 ctacgaatac gaaatcaact tgaatcttgg catattgaat tatattgctg aaactgcctg
     1981 caaatttggg caggaggaaa ttggcgataa aagcaaggag gcttttgaga aaaatttaaa
     2041 atgttatttt actcagattt atcaaaactt cgaaccttga tgtttatgat aaaagcgacg
     2101 acgtgttttc ccagggctgt cgaatggata tttgctccat ttgtaattac tcgtatgcct
     2161 cggacaggtc aatgcctcta tctaaccttc gatgatggcc ctcattcaca aattaccccg
     2221 aaagttttgg atattctcaa tcgctttcaa gtaaaggcca cttttttttg cttagggaaa
     2281 aacattcgag cttatcccga agtctttcag gaaataaaaa ataatgggca taacataggg
     2341 aatcattcat tcagtcataa aaatggatgg aagaccgcca ataaggagtg tttcaaagac
     2401 atcgaggctt gcagggagct aataccgacg aatctatttc gtccgccata cggaaggatg
     2461 agggtttcac aggttagagt gctttcacga caataccaaa tcattttgtg gacgttgctg
     2521 agcggtgatt acaatcgaag cctttcatcg gaagaaatac ttcgaaccct gcttcgacga
     2581 acgcgatcgg gcgatatcat cgtttttcac gataacgaaa aggcatttcc ttgcctaata
     2641 gaggttttac catcatatct ggaggccatg ctcgaaaaag gttttacttt tgatgtaatt
     2701 cagtcgaaac tttaagtatg gagtcccgaa gatattttat aagaattcta aagaaggcga
     2761 ttgataatgc catgctggcg cttggtcttg gcctgttgct cttgatgtgt cgctgtgcta
     2821 atcctgtggt accacaggga gggccaaagg atacggatcc accaaagttg ctttatgccg
     2881 ttcctcccaa tctaagcact cactttacag catctcacat taagttaacc ttcgatgaat
     2941 atgtggttct gaaagaaata aacaaaaaat ttctgatgtc gcctccttca ctcaatcttc
     3001 cagagttcaa ggtaaagggg aaatcactcg agatcgactt gaaggatact ttaaggccca
     3061 ataccaccta ttctttgtat tttgccgatg ccatcaccga ccttcatgag aataatcctc
     3121 tgcccgattt tcaatatgta ttttcgaccg gtgcagtgat cgattcatta actatgacgg
     3181 gttcggttgt aaatgctttc aacctgaaac ccatggaaaa tatggtcgtt atgctctatt
     3241 acgccaataa cgataccctt ccgctcgaac aacttccttt gaaagtccca ccttcttatg
     3301 cagcccgcac tcgcaaggat ggttcgttca acctcaatta tttacgcgac gaaaaatatc
     3361 tgctctttgc attgatt
//
LOCUS       DAORPD010000064         3337 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_5044_length_3337_cov_2.388787, whole genome shotgun sequence.
ACCESSION   DAORPD010000064 DAORPD010000000
VERSION     DAORPD010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5044_length_3337_cov_2.388787"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1009)
                     /locus_tag="PK976_01275"
     CDS             complement(<1..1009)
                     /locus_tag="PK976_01275"
                     /inference="COORDINATES: protein motif:HMM:NF013954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HRC66550.1"
                     /translation="MNEIRNWAKAAVLVFIFSSGFYFSIEAQDTSNAGFKIREAYIQK
                     YKALAIREMVEYGIPASIKLAQAVLESGAGNGELAKIANNHFGIKCHKEWTGDKFFKD
                     DDSVKECFRKYESVEESYRDHSLFLRSRERYSNLFRFDICDYKAWAYGLKNAGYATNP
                     AYPQLLIKIIEEHQLTRYDHPGSDTLQLANIETDSSIARGGTADNNLTSERGPVDVSP
                     VKITKEGRKIYKNNDSKYIIYRQDDTPIRIAQEFDLYTWQIYDFNDLELDNPIKPGDM
                     VYIEKKKRKASEEFYIVKPGDDMHAISQKFGIRLQNLYELNRMNFGTEPQQGDTLWLN
                     KK"
     gene            1112..1729
                     /gene="rsmG"
                     /locus_tag="PK976_01280"
     CDS             1112..1729
                     /gene="rsmG"
                     /locus_tag="PK976_01280"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005846883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HRC66551.1"
                     /translation="MELISKYFPGLSEKQLNQFDMLKELYSSWNQRINVISRKDIEQL
                     EMHHILHSLSIARIIRFEPDTQILDAGTGGGFPGIPLAIYFPDVNFTLVDSIGKKIKV
                     VDEIILALGLENIETRKERIENLHEKYDFIVSRAFGQLSDLLRFSENLIRRDDINFLQ
                     NGILYLKGGKIEDELNRLGRPFHVYRLSNYFYEPWFETKKIVHIY"
     gene            1829..3289
                     /locus_tag="PK976_01285"
     CDS             1829..3289
                     /locus_tag="PK976_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HRC66552.1"
                     /translation="MQQDIRILKPTALWKNFFSLTQVPRPSKNEERVRQFLIDFGKKL
                     GLETLTDEVGNVIIRKPATKGMENRKGIILQGHMDMVPQKNSDNPHDFNHDPIEAWVD
                     GEWVRARGTTLGADNGIGVAAAMAILESTDIPHGLIEVLITTDEETGMTGAQNLKPGL
                     LHGDILLNLDSEDEGELYVGCAGGVDGTFLFPYSKETLTSGMKAFKLSVTGLRGGHSG
                     LDINLGRGNANKILFRFLKHAQNHHQLRLACVEGGSLRNAIPREAFANVVIPETETEK
                     FLHCLDQYTEIIKNELESAEPNLSFTCENAPMPSHLIGQDTQWRLINAIYGCPNGVMR
                     MSNTFSGVVETSTNLANVKMNDNEVSVLCLLRSLVDSAKEDLSEMMASVFELAKANID
                     FSGSYPGWKPNPDSTILQLAKNIYKDKFGKEPEVKVIHAGLECGILGAAYPHWDMISF
                     GPTIRNPHSPDEKVNIPSVKRFWEFLLEILKTAPLK"
BASE COUNT          966 a          724 c          684 g          963 t
ORIGIN      
        1 tttttttgtt caaccataaa gtatctcctt gctgaggttc ggtaccaaag ttcattcggt
       61 tcagttcata gagattttgc agacggatgc caaatttttg cgaaatggcg tgcatgtcat
      121 caccaggttt cacgatataa aactcctcac tggcctttcg ttttttcttt tcgatgtaca
      181 ccatatctcc gggctttatc gggttatcca gttcgaggtc attaaaatcg tatatctgcc
      241 atgtatagag gtcgaattcc tgagctattc ttatgggtgt atcatcttgc cgataaataa
      301 tatacttact gtcgttattc ttgtatatct ttcgaccttc tttggtaatt tttaccggtg
      361 aaacatccac gggacctcgc tctgaagtaa ggttattatc tgccgtccca cctctggcta
      421 ttgatgaatc ggtttcaata tttgccagtt gcaaggtatc ggaaccagga tggtcgtaac
      481 gtgtgagctg gtgttcctca ataattttga tcaagagttg aggataggct ggattggtgg
      541 catacccggc atttttcagc ccataagccc aggccttgta atcgcaaata tcgaatctaa
      601 acagattgga atagcgctca cgtgaacgca aaaacaaaga atgatcgcga taggattcct
      661 ctaccgactc atatttcctg aaacattcct ttacactgtc atcgtccttg aaaaatttat
      721 caccagtcca ttctttatgg cattttatac cgaagtgatt gttcgctatt ttggctaatt
      781 ctccattacc tgcgcctgat tcaagaacag cctgagccaa ttttatgctt gccggaatgc
      841 catattctac catctcgcgt atggccagag ccttatattt ctggatatag gcctctcgta
      901 ttttaaatcc tgcattactt gtatcttgtg cctcgataga gaaatagaag ccggaagaaa
      961 aaataaaaac caacacagcg gctttcgccc aatttctaat ttcattcata cggcaaaatt
     1021 aataattgcc cgaaagaatc aaccatgaaa gcatttggcc gcaacccccg atttgcatag
     1081 ttttgcaccc atcaactcaa tcgagcattt aatggagctc atttcgaaat atttcccagg
     1141 gctaagcgaa aagcaactga atcaattcga tatgctcaaa gaactctatt catcctggaa
     1201 tcagcgtatc aacgttattt cacgcaagga cattgagcaa cttgagatgc atcatattct
     1261 tcattcctta agtattgccc gaatcatacg ttttgagccc gatactcaaa ttcttgatgc
     1321 tggcacagga ggaggattcc ccggaattcc tttggcgata tattttcccg acgttaactt
     1381 tactctggta gattccatcg gaaaaaagat aaaggtggtg gatgagatca tacttgcctt
     1441 gggactcgaa aacatagaga ctcgcaagga acgcatcgag aatctgcacg agaaatatga
     1501 ctttattgta agcagagctt tcggacaact ttccgactta ttgaggttct cagaaaacct
     1561 cattcgccgc gatgatatca attttcttca aaatggcatt ctctatctga agggagggaa
     1621 aatagaagat gagctgaatc gattgggaag accttttcac gtttatcgtc tgtccaatta
     1681 tttttatgag ccttggttcg agacaaaaaa aattgttcat atttattaac ttgttctcca
     1741 tatccatatt ctttttcttt caaggataat atttctctaa ttttgcagct acgcaagttc
     1801 aatttattgt aattaaacca aactatttat gcagcaagat attagaattt taaaaccaac
     1861 tgccctctgg aagaattttt tcagcttgac acaggttcca cggccttcga agaatgaaga
     1921 acgcgtcaga caattcttga ttgatttcgg caaaaaacta ggtttagaaa ccctgaccga
     1981 cgaggttggt aacgttatta tccgcaagcc tgcaacaaaa ggcatggaaa atcgcaaagg
     2041 cataattctg caaggccata tggacatggt tccgcaaaag aacagcgaca atcctcatga
     2101 tttcaaccac gatcccatcg aagcatgggt tgatggcgaa tgggtgagag ctagagggac
     2161 tactctgggt gccgacaacg gtattggagt agccgcagct atggcgattc tcgaatccac
     2221 cgacatacca catggcctca ttgaggtact aattacaacc gatgaagaaa caggaatgac
     2281 gggcgctcag aatctaaaac cagggttatt gcacggcgac atcttgctga atctcgattc
     2341 ggaagatgag ggcgaacttt atgttggttg tgcgggagga gtggatggca cattcctttt
     2401 cccctattcg aaagaaactc tcacctcagg catgaaagca ttcaaacttt cggttaccgg
     2461 gctccgaggt gggcactctg gccttgacat caatttagga agaggaaatg ccaataaaat
     2521 tctcttccgt ttccttaaac acgctcagaa ccatcatcaa ctgcgcttag catgcgttga
     2581 gggaggaagc ttacgtaacg caattcctcg cgaagcattt gctaatgtgg ttattccaga
     2641 aacagaaacc gaaaaattcc ttcattgcct cgatcaatac acagaaatta tcaaaaatga
     2701 actcgaatca gctgaaccta atctgagctt tacatgtgaa aatgccccaa tgccttcaca
     2761 tttgataggt caagatactc agtggcgact gatcaatgcc atttatggct gtcccaatgg
     2821 agtgatgagg atgagcaata cattctccgg tgtagtcgaa acctcgacta atttagcaaa
     2881 tgtgaaaatg aacgataatg aagtttctgt tctatgtctg ttacgcagct tagtcgattc
     2941 tgcaaaagaa gatctttcag aaatgatggc ttcggttttt gaactagcca aggcaaatat
     3001 cgacttctcg ggttcgtatc cagggtggaa gcctaatcct gatagtacaa tattgcaact
     3061 ggcgaagaac atttacaagg acaaatttgg gaaagaaccc gaggtaaagg tcattcatgc
     3121 cggcctcgaa tgtggaatat taggcgcagc ttatccccat tgggacatga tctcgtttgg
     3181 gcctactatt cgtaaccctc atagccccga cgaaaaagtg aatatcccca gcgtgaagcg
     3241 cttctgggaa tttctgctcg aaattcttaa aactgccccc cttaagtaga aatttataag
     3301 ttaaatattt acttaatcag cccctgtaca ggggctt
//
LOCUS       DAORPD010000065         3336 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_5045_length_3336_cov_2.293203, whole genome shotgun sequence.
ACCESSION   DAORPD010000065 DAORPD010000000
VERSION     DAORPD010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5045_length_3336_cov_2.293203"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="PK976_01290"
     CDS             complement(<1..685)
                     /locus_tag="PK976_01290"
                     /inference="COORDINATES: protein motif:HMM:NF023504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66553.1"
                     /translation="MAGYKETPRQKMIAMMYLVLTAMLALNVSKDIIQAFVTVNESME
                     QTNNSFDKKLRETYSKFESQATIGGASAKEWYDKALIAKKLSDSLVNYLNHMKNELIV
                     FSDRSIKTLEQADTTPLIRIKAKDNFDDPTRFFLGTDVSKGKAEDLKRVFKDYKEKMT
                     QLVKDEDHPKLQLGLITEGKFRDEYGAVQTWEKHYFYHTILSADLVILNKYIAEVRNA
                     EFDVVSLLSS"
     gene            complement(736..1935)
                     /gene="gldL"
                     /locus_tag="PK976_01295"
     CDS             complement(736..1935)
                     /gene="gldL"
                     /locus_tag="PK976_01295"
                     /inference="COORDINATES: protein motif:HMM:TIGR03513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldL"
                     /protein_id="HRC66554.1"
                     /translation="MNLFDLVKSKKYKVFMARLYGIGASVVIIGALFKITHIRGADVM
                     LFVGLITESIIFFFSAFEPPYIDPDWSLVHPELAGLYHTEEEIEMMKQKGLIKEKKEE
                     EASLTGELDKMLKEANIQTDVIASLGQGLNNLNTTLKELNNISEVAVKSTNLVNTIDH
                     VNQNAQALSQSYKKTAEVLDKESELTQEHVTNLGGMTQNSGLASKAYGEIANKLNEES
                     NIRSALNQNIHSASQSAANLVEKYNETAQTLKNAADAIDVGENASVFKEKLSEINTKL
                     TSLNAAYELQMKAAHENASVTSTSIQTINELISKLMGSTEATGQFTQNLSALSKALES
                     QINGSNLQTQKTEEVSQAYKELFDRINAAIAHTENYQKEAKNLAEKMADLTRIYGNML
                     SAMTIKV"
     gene            complement(1982..>3336)
                     /locus_tag="PK976_01300"
     CDS             complement(1982..>3336)
                     /locus_tag="PK976_01300"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRC66555.1"
                     /translation="SGELTGVPDRPKFYQPDPYGMTFIPLGSFTMGTGDQDVPYANVH
                     HPKTISVAAFYMDETEITNNEYRQFVYWVRDSLAHTILGNAGSDYGEHFVSDKQGQPI
                     QYGTTQTYYKINWKQKIDWEGKRLKQGEPNPLDEMYLPEEERFFRRKEIDTRKLFYRY
                     YWVDLPKSARPENRYIFNRDQQGKITDEGGTYPQNIVAEGNRRAFVKEEVVPVYPDTL
                     VWISDFTYAYNEPMAKTYFWHPAYDHYPVVGVNWKQAEAFCVWRTEYMNSYLSTRKRE
                     ALVNDFRLPTESEWEWAARGGNELNPYPWGGPYLRNQKGCFLANFKPNRGNYVADGGL
                     QTVIVAHYPPNDFGLYDMAGNVAEWTNSAFDESSYNFTWDLNSSYTYHANYDDPAVMK
                     RKVVRGGSWKDIGYFLQVSTRTFEYQDSAKSYIGFRCVQSYLGRNKDDNPSKSSEIYN
                     "
BASE COUNT          855 a          668 c          691 g         1122 t
ORIGIN      
        1 agctcgacaa aagagaaacc acatcgaatt cggcattgcg tacctcggct atatacttgt
       61 taaggattac aaggtcggca gaaagaatgg tatggtaaaa gtaatgtttt tcccaggttt
      121 gtactgcacc atattcatct cgaaattttc cttccgtaat caaacctaat tgaagttttg
      181 gatggtcttc gtcttttacc agttgagtca ttttttcctt ataatcctta aaaactcttt
      241 tcaaatcttc ggctttccct ttcgaaacat cggttccaag aaagaatctt gtaggatcgt
      301 caaaattatc tttggccttg atacggataa gaggagtggt atcggcctgt tctaaagtct
      361 ttatactacg gtcggagaaa acgattaatt cgtttttcat gtgattcaga taatttacta
      421 agctatccga caattttttt gcaatcaatg ccttatcgta ccattctttg gccgaagctc
      481 ctccaattgt tgcttgactt tcgaatttcg aatatgtttc tcttagtttt ttatcgaagc
      541 tattgttggt ttgttccatg ctttcattca cggttacaaa cgcctgtatg atgtctttcg
      601 aaacgtttaa ggctaacata gcagtgagta ccaaatacat cattgctatc atcttttgcc
      661 gtggggtttc tttgtaacca gccattagaa ataggtttta gtgaataaca attattatat
      721 agggttagga acagcttaaa ctttaatagt cattgctgaa agcatattgc cgtaaatacg
      781 ggtaaggtca gccattttct cggctaggtt tttggcttct ttttgataat tttctgtatg
      841 agctatagcg gcattgattc tgtcgaacaa ttccttgtat gcttgtgaaa cctcctcagt
      901 cttctgggtt tgcagattag aaccattgat ctgagattca agggctttgc tcaaggcaga
      961 aagattttgt gtaaattggc ccgtggcttc ggttgaaccc atcaacttgc taataagttc
     1021 gtttatagtt tgaatgctcg tagaagtcac actggcgttt tcatgggcag ctttcatttg
     1081 aagttcatag gctgcattaa ggcttgtcaa ttttgtattg atttctgaaa gcttttcttt
     1141 gaaaaccgag gcattttcac ctacatcaat agcatcggca gcatttttaa gagtttgagc
     1201 cgtttcgtta tacttttcaa caagattagc agccgattgt gaagctgagt gaatattctg
     1261 atttagtgcc gatctaatgt tgctttcttc attcaattta ttggcaattt caccataagc
     1321 cttactggct aaaccagaat tttgagtcat tcctcctaga ttagtcacat gttcttgagt
     1381 caattccgat tctttatcaa gaacttcggc tgtcttttta tagctttggc tcaatgcctg
     1441 agcattttgg ttcacatgat ctattgtatt taccagattt gtggatttga ctgctacttc
     1501 ggaaatgttg ttcaattctt tcagtgttgt attcaaatta ttcaagcctt ggccgaggct
     1561 tgctattaca tctgtttgaa tattcgcctc ctttagcatt ttatcgagtt cgcccgtaag
     1621 gcttgcctct tcttcttttt tttccttaat gagtcctttt tgtttcatca tttcaatttc
     1681 ttcttcagta tgataaagcc ctgcgagttc tggatgcacg aggctccaat cggggtcgat
     1741 ataaggaggt tcgaaggctg agaagaagaa aataattgat tcggtgatta aaccaacaaa
     1801 aagcatcaca tcggccccac gaatatgggt aattttgaag agtgctccaa taatgacaac
     1861 ggaagctcct attccataga gccttgccat gaaaactttg tacttcttgc tttttacgag
     1921 atcgaacaga ttcatgattt ttattgtttt gttttatggg gttaatttgg attttctcta
     1981 attagttata aatttcggat gatttggagg ggttgtcatc tttattgcga cccagataag
     2041 attggacaca gcggaagcca atataagact ttgcagagtc ctggtattcg aaagtccgtg
     2101 tagatacctg aaggaagtaa cctatgtctt tccatgaacc gcctcttact actttgcgtt
     2161 tcatcacggc aggatcatcg tagttggcat gataggtata agatgagtta agatcccagg
     2221 taaaattata ggaagattcg tcgaatgcgc tatttgtcca ttcggctacg tttccggcca
     2281 tatcgtatag gccaaagtcg ttaggtgggt aatgagccac gataacagtt tgtaagccac
     2341 cgtcggccac ataatttcct ctattaggct taaagttggc taaaaaacag cctttttggt
     2401 tgcgtaaata tgggcctccc catggataag ggttgagttc gttgcctcca cgggcagccc
     2461 attcccattc gctttcggta ggcaatctga aatcgtttac caatgcttcc cttttacggg
     2521 tcgaaagata gctattcata tattcggttc tccatacgca gaatgcctcg gcttgtttcc
     2581 agttaacacc aactacgggg taatgatcgt aagcgggatg ccaaaaataa gtcttggcca
     2641 tgggttcatt ataggcataa gtaaaatcgc ttatccagac caaagtatcc ggataaacgg
     2701 ggacaacttc ttcttttaca aaggctcttc tgtttccttc agccactata ttctgcggat
     2761 aggttcctcc ttcatcagta attttccctt gctggtcgcg attgaaaata tagcgatttt
     2821 cgggcctggc agatttgggg agatcgaccc aataataacg gtaaaagagc tttcgggtat
     2881 caatttcctt acgacgaaag aagcgttctt cttcgggcaa gtacatctca tcgagcggat
     2941 tgggctctcc ttgtttgagg cgtttccctt cccagtcgat cttctgtttc caattaattt
     3001 tgtaataggt ttgagtggta ccatattgaa tgggttgtcc ctgtttgtcg gaaacaaagt
     3061 gttcgccata atccgaaccg gcattcccta agatagtatg agctaatgaa tctcgtaccc
     3121 aataaacaaa ctgccggtat tcattgttgg taatttcggt ttcgtccata tagaatgcgg
     3181 ctaccgagat ggtttttgga tgatgaacat ttgcataggg cacatcctga tcgcccgtac
     3241 ccatggtaaa actgcctaaa ggaatgaatg tcataccgta agggtcgggt tgataaaact
     3301 tcggcctgtc gggaacaccg gtcaattccc cgctgc
//
LOCUS       DAORPD010000066         3334 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_5051_length_3334_cov_2.562367, whole genome shotgun sequence.
ACCESSION   DAORPD010000066 DAORPD010000000
VERSION     DAORPD010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5051_length_3334_cov_2.562367"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..380)
                     /locus_tag="PK976_01305"
     CDS             complement(165..380)
                     /locus_tag="PK976_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HRC66556.1"
                     /translation="MSIGVIFAGAIGQTELIIIIAVILLLFGGKKIPELMRGLGKGVR
                     SFKDGMSGKEDEEISNPGNKGDKPEKS"
     gene            complement(431..1684)
                     /locus_tag="PK976_01310"
     CDS             complement(431..1684)
                     /locus_tag="PK976_01310"
                     /inference="COORDINATES: protein motif:HMM:NF014592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HRC66557.1"
                     /translation="MSITEIHLSDFIYDLPQQKIAMFPAEPRDSSRLLVYNPNNDFLA
                     EDIFENVIEYLPHDSLLIFNETRVVHARMIFRKPTGGQIEVLCLNPVSPSSEIETAFQ
                     QTSPVIWKAYVGNAKRWKSGVLKMDFEYGNQHRGIFQVRQVGRKEGIFFLEFSWEPEE
                     LTFSEVLEAAGKIPLPPYIRRKAIPADDIRYQTVYARCDGSVAAPTAGLHFTEEILQH
                     LPKKDIQPLNLILHIGAGTFKPVDQDDVRQHVMHTEEIIVKCETIERLLISPEKLRIV
                     VGTTGVRTLESLYQTGKKLLQGIPLHRAVHTNQWDPYEGNLPETSMALEAILDALDKE
                     GQDAFRGTTSLMIVPGYQFKFTDILITNFHQPRSTLLMLVAAFCGNSWRKAYNYALDH
                     QFRFLSYGDACLFFKNNVQLNGIAV"
     gene            2647..>3334
                     /gene="pssA"
                     /locus_tag="PK976_01315"
     CDS             2647..>3334
                     /gene="pssA"
                     /locus_tag="PK976_01315"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HRC66558.1"
                     /translation="MASIRKNIPNLLTSFNLLSGVIGLALLIRGNLPFASMMVLIAGI
                     FDFLDGMVARLLSVKSLLGKELDSLADVVSFGVLPGFILYHIVISQNLLWPLIAFPPL
                     LIPVFAALRLAKFNIDRRQEEEFRGLPTPAMAIFIASLEAPYGFSTLFLNSILSAFLF
                     HPLTLSLLALLLCFMMISEIPLFSLKMKSFSFKEYPYPMVFLCVSLMLVLIFKTAGLG
                     FSILTYVFLSL"
BASE COUNT          916 a          651 c          655 g         1112 t
ORIGIN      
        1 agaattttag ggtttgatat ggggagactg ggagacaaag aggatgtgat aatggggaaa
       61 aatcaggacc agcaaatgaa agaccccgcc aaaacgtcta tctctggcag attagactaa
      121 tagaaatttt attcagagga agggggaata gttgcccgaa gaaatcagga tttctcgggt
      181 ttatcgcctt tattccctgg attagaaatc tcttcgtctt cttttccgga cataccatct
      241 ttgaaacttc taaccccttt gcccaatcct cgcatcaatt caggaatctt tttacctcca
      301 aaaagaagca atatgacggc aatgatgata atgagctcgg tttgtccgat ggcacccgca
      361 aaaataaccc ctatgctcat gatattgatt tttatttcgt taatgcaaag gtataggaat
      421 tttgctttaa tcatactgct attccattaa gttgcacatt gtttttgaaa aatagacagg
      481 catctccata actcaagaaa cggaactggt ggtcgagcgc ataattatag gcctttctcc
      541 atgaatttcc acaaaatgca gctaccaaca ttaaaagggt actacgcggc tgatgaaaat
      601 ttgttatcaa gatatcggtg aatttgaatt gatatccggg aacaatcatc aaggaagtgg
      661 ttccacgaaa tgcatcttga ccttccttat cgagagcatc aagaatcgcc tctaaagcca
      721 tagaagtttc gggcaaatta ccttcgtatg gatcccattg atttgtatgt accgctcgat
      781 gcaaggggat tccttgtaag agttttttgc ctgtttgata aaggctttcc aatgttctaa
      841 cgcctgtggt tcccacgact atccggagct tttctggtga aattagcagc ctttcgatgg
      901 tttcgcactt cacgataatt tcctcggtgt gcatcacatg ttgtcgtaca tcatcctgat
      961 caactggttt aaaagtgcct gctcctatat gcagaatcaa attcagaggc tgaatatctt
     1021 ttttggggag atgttgaagg atttcttccg taaaatgcaa accagcagtt ggtgccgcca
     1081 ccgatccatc acaacgtgca taaacggttt gatatcttat atcatcagcg ggtatagctt
     1141 tgcgacgaat gtatggtggg aggggaatct tgcctgctgc ttctaaaact tccgaaaaag
     1201 ttaactcctc aggctcccat gaaaactcaa ggaaaaagat gccctctttt cttcctactt
     1261 gcctaacctg aaagatgcct ctgtgttgat ttccatattc gaaatccatt ttcaatactc
     1321 ccgatttcca gcgttttgca ttgccaacat acgccttcca tatcactggc gaagtttgtt
     1381 gaaaagcagt ctcgatttct gaggaaggac ttactggatt cagacataat acttcgattt
     1441 gcccacccgt tggttttcga aagatcatcc tggcatgtac tacacgagtc tcgttaaaaa
     1501 ttagcaaact atcgtgtggg agatattcta taacgttttc gaaaatatct tcggcaagaa
     1561 agtcattgtt gggattatat accagcagac gtgaactatc gcggggttcg gcaggaaaca
     1621 ttgctatctt ctgctgtggt aaatcatata tgaaatcgct gagatgtatt tctgtaatcg
     1681 acattcattt attccattta gactatataa gaagctttat aaaggacttc aacaactttg
     1741 cgaccaataa tgtatttcaa aatcatatca gattggaaca attacataca tatagcttcg
     1801 attctcttga gccgagaaat aaacctgact ttcattgatt cgattatcct ttgatttcgt
     1861 agctgtggtt tatagcttct ccagcacaaa taaaaatttt gtgcaaaaat acattctaca
     1921 ctcgaatctc cattcggctt attgtcgtaa cttttctcta cggctatatt tcggattttt
     1981 gattcttaat tttaaatccg attcaattcg cgtaagttca aatccctgtg acaaattaat
     2041 cttcaattct taatttgttt acgtaagccc tcataaaaaa atacagttta ctgtgagaat
     2101 ttaacccata accgtttcaa aggtgctata agggagattg tctcttggaa gggatatcag
     2161 tttttcaaat ttctctcaac gtcagggatt ttacgtgaaa aaatagtaag tcggagtgaa
     2221 gtcaaacgtg atgcagattg agatgaattt ttcgagccgg ggaaaatgtt ttattcttac
     2281 aaggctgcag tatggtttta ctttgatgtg tttatgctac taaaaactct gggtttggaa
     2341 aggtgagagg atctcgggaa ccaggtaatg agaagtaaaa ttcgtataat agttgatgag
     2401 cttttctttt ttatgattca gcccatgctt tgaaacagac tcatattttc atagccttgg
     2461 gattttcgat gtttgttacg aattatatta tttcgattcc aacataatac ttttttgaat
     2521 tcgaaggatg cttccttacg aagcagatta tgaataggat gagaatagct tgcctcatat
     2581 cgaattcata gaatgacgca gcaaattatt tgaaacctca gtacttttgc cacattattt
     2641 taatttatgg caagtattcg gaagaacata cccaatttgc tcactagttt caatctttta
     2701 tcaggggtga taggattggc tttgttaata agggggaact taccttttgc ttcgatgatg
     2761 gtcctaattg ccggcatctt tgattttttg gatggaatgg tggcgcgctt attgagtgtg
     2821 aaatccctgt taggcaaaga gctagattcc ttggccgatg tagtttcatt tggcgtgctt
     2881 ccgggattta ttctgtacca tatagtaatt tcacaaaatc tactttggcc gctgatagcc
     2941 tttccccctt tgttaattcc cgtatttgcg gctttaagat tggccaaatt caacatcgat
     3001 agaaggcagg aagaagaatt cagaggtttg cctacgcctg caatggcaat ttttatcgct
     3061 tccttagaag ctccttatgg attttcgact ttatttctca actcaatcct ctccgccttt
     3121 cttttccatc ccttgacctt gtcgctgctg gctttgttac tttgcttcat gatgatttcg
     3181 gaaatcccct tgttcagtct taagatgaaa agtttttctt ttaaggagta tccttatccg
     3241 atggttttcc tttgcgtttc cttgatgctt gttcttatct ttaagactgc tggattgggt
     3301 ttttccattt tgacctatgt tttcctttca ctgg
//
LOCUS       DAORPD010000067         3327 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_5065_length_3327_cov_2.351773, whole genome shotgun sequence.
ACCESSION   DAORPD010000067 DAORPD010000000
VERSION     DAORPD010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3327
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5065_length_3327_cov_2.351773"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1080)
                     /locus_tag="PK976_01320"
     CDS             complement(<1..1080)
                     /locus_tag="PK976_01320"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HRC66559.1"
                     /translation="MGKVKQLAGQTAVYGLSSILGRILNYLLVPLYTRVFLPHESAVY
                     VELYAYMAILAVILTYGMETAFFRYSETHSKPKMVYSTAFNSIVITTILFLLFTLTFR
                     DSIASWLGYEGKTEYIIYFLLILGFDALSAIPFAWLRAQQKAWTFAGIKLANIFINIA
                     LNLILILLLPYLYNKYGWSWLGIFTGGKPEVSAIFISNMVASFATLLLLWPAIRKASL
                     TIDLGLWKEMFLYAFPLLVYNLAGVINETIDRILLKHFLPPETAIHDLGVYGMCFKFS
                     VFIVIFIQAFRYAAEPFFFKQAKEKDAPETYAMVMNYFIIVCFGMFAGIMLFIDVIKY
                     FVGPMYYAGLPIVPLLLMGHVFLGIY"
     gene            complement(1208..1600)
                     /locus_tag="PK976_01325"
     CDS             complement(1208..1600)
                     /locus_tag="PK976_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRC66560.1"
                     /translation="MGYNQPSIPTEMNNFLSSARDFSYSGGGIVVKDGKNKENILSSL
                     TDSGIGIYEENLENLSRIDFTRSSAGTHGEEPMGLGLIFLKNSWVIVKLRFWSKARKI
                     RALPWRFTFRPCDSMKNITRNERFILPI"
     gene            1779..2219
                     /locus_tag="PK976_01330"
     CDS             1779..2219
                     /locus_tag="PK976_01330"
                     /inference="COORDINATES: protein motif:HMM:NF013698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRC66561.1"
                     /translation="MRTVSGLDGHKDSVFVCFLDEQDKKILEKHYGTLTPDLTTLRDA
                     LIEHDCGSIAMESTSIYWMPVWHVLESDFSLKLVNPYFIKQLPGRKSDVKDAQWIATV
                     LHKGLLRGSLIPCKEIKKLLKYNPVTWVALFRSGNTMHHLLNGL"
     gene            complement(2350..2499)
                     /locus_tag="PK976_01335"
     CDS             complement(2350..2499)
                     /locus_tag="PK976_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66562.1"
                     /translation="MLNGHAVPAATAQAVSKADILTKMFTYFKNAVHNSPPAKQYIES
                     RKLDY"
     gene            2533..2769
                     /locus_tag="PK976_01340"
     CDS             2533..2769
                     /locus_tag="PK976_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66563.1"
                     /translation="MVLPLHDKINNIKAFAVGLAIGDKTVCRVGFSSKPACSGSYLRG
                     RGSRYGYVYRVSGCNTATRLPLTAVVLSRLFPWF"
     gene            2987..>3327
                     /locus_tag="PK976_01345"
     CDS             2987..>3327
                     /locus_tag="PK976_01345"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRC66564.1"
                     /translation="MGISHYNKIENGQHEASVELLDKLAKLYGVSIDTIVHMEGEVPK
                     EIVIEDKTTSEQIRLIQELDEKDKGIIFSMIDIMLTKKKLKHFFQKNVAALLTKTPGL
                     AGRFYYLTKAIL"
BASE COUNT         1048 a          666 c          661 g          952 t
ORIGIN      
        1 gtaaattccc agaaagacat gccccatgag taaaagggga actatgggca ggcctgcgta
       61 atacataggc ccgacgaaat acttaatgac gtctataaac agcataatgc ctgcgaacat
      121 tccaaaacag acgatgataa agtaattcat caccatagcg taagtctcgg gagcatcctt
      181 ttcctttgcc tgcttgaaaa agaaaggctc ggccgcataa cgaaaggctt gaataaaaat
      241 tacaataaac acactgaatt tgaaacacat gccataaaca ccaagatcat gtatagcagt
      301 ctcgggagga agaaaatgct tcaatagtat ccggtcgata gtttcgttga taaccccggc
      361 cagattatag acaaggagcg gaaaagcata cagaaacatt tccttccaga gccctaagtc
      421 gatagttaaa cttgccttgc ggatagccgg ccaaagcagc agcaatgtgg caaagctcgc
      481 caccatgttc gaaataaaaa tcgcactcac ctcgggtttc cctcctgtaa atataccaag
      541 ccagctccat ccatacttat tgtataaata tggtaacaac aggataagaa tgagatttag
      601 ggcaatatta atgaaaatat tcgccagttt tattccagca aaagtccatg ccttttgctg
      661 ggctcgtagc caagcaaacg gaatggcact caatgcatcg aatccaagaa taagtaaaaa
      721 ataaatgatg tattctgttt taccctcgta accaagccac gaagcaatgg aatcgcgaaa
      781 agtaagggta aacaacagga acagaatggt tgttatgacg atggaattga atgcagtact
      841 ataaaccatc tttggttttg aatgggtttc ggaatagcga aagaaagccg tttccatccc
      901 atacgtaaga ataacagcca aaatggccat gtaagcatat agttcaacat aaacggccga
      961 ttcgtggggt agaaatactc tggtatataa gggaaccaga agataattca atatccttcc
     1021 taatatgcta ctaagtccat atactgcagt ctgccctgcc aattgcttta cttttcccat
     1081 ttatcaggct gatatgaata atccttcgat ctcgaataat cccagaacgc agatagaaaa
     1141 atgcaaaaat ggtattccat ctgaaatatc tatatttttc tctgatctgg ccaagtttct
     1201 catcaaacta tataggtaat ataaatcttt catttcgcgt tatgtttttc atgctatcac
     1261 aaggccgaaa tgtaaaccgc caaggtagtg cccttatttt tctggctttc gaccaaaatc
     1321 tcaacttgac gataacccac gaattcttca gaaaaatcaa acccaaaccc attggttctt
     1381 cgccatgagt gccggcggaa gatcgagtga aatcaatcct tgacaaattt tccaagtttt
     1441 cttcgtatat cccaatgcca gaatcggtaa gtgacgaaag gatattttcc ttgtttttcc
     1501 cgtcttttac gacgattcct cctcctgaat atgaaaaatc cctagcactc gataaaaaat
     1561 tattcatttc agtgggaata gaaggttgat tgtatcccac aaaagtaagg acaattaaac
     1621 tttattgcct ggttttttaa aagaagaaat gttttacaaa cttacataaa aaaataattt
     1681 tcatacgccg cacaacaaaa atcaaggcca tgattgttct ataagtaatc aacgtactca
     1741 ataagtagta caattgttta atttaaaact atttatatat gagaacagtc agtggccttg
     1801 acgggcacaa agatagtgta tttgtatgct ttttggacga acaagacaaa aaaattttag
     1861 aaaaacatta cgggacgctc actcccgatt taacgacctt gcgtgatgca ctgatagaac
     1921 acgattgtgg aagcattgca atggagagta ccagcattta ctggatgcct gtatggcatg
     1981 ttcttgaatc agacttttcg ttaaaattgg tcaatcccta tttcatcaaa cagttacccg
     2041 gccgcaagag cgacgtaaag gatgctcagt ggatagctac agttttacat aaagggcttt
     2101 tacgcggcag tctgatcccc tgcaaggaga taaaaaagct tctaaaatat aatcctgtta
     2161 cctgggtcgc tttgtttcgg agcggaaaca caatgcacca tttgctaaat ggcctgtaag
     2221 ttttattgcc tgttttgctg taagccctgc cttctgtaat aaaccgacct ataaacactg
     2281 ttttattaat gtttcgtcct ttgtttcctt gtggtggaac tggtcggagt tatagccgat
     2341 ttcgagtttt taataatcca gcttgcggct ttcaatatac tgttttgctg gcgggctgtt
     2401 atgcacggcg tttttaaagt aggtgaacat cttggttagt atatcagcct tgcttaccgc
     2461 ctgggctgtg gctgccggga cggcatggcc gttcaacatt ccggctgctt ttaaaattgc
     2521 attgtgttta tcatggttct cccgctacat gataaaatca ataacatcaa ggcttttgcc
     2581 gtgggtcttg caattggaga taaaacagta tgccgtgtgg gttttagtag taaacctgca
     2641 tgctcggggt cttatcttcg tggaagaggc agcagatacg gttatgttta tcgggtttca
     2701 ggttgtaata ctgcaacacg gttgccattg acagccgttg ttttatctcg cttatttcca
     2761 tggttttaaa aatttacttc cattttgtac atcgcaaaac taaatataaa ttgactattc
     2821 ggcaaatata tttacgacta tttggatatt tattgccatt tattgactta atggctatct
     2881 ttgaggcatt ctaaaaacat tgactatatg agtttagccg ataacataaa gcatctcagg
     2941 gaaaacaagg gggttacagc aaaagcaggt agccattgag attggtttgg ggatttcaca
     3001 ttacaacaag attgagaacg ggcagcatga agcttcggta gaactgttgg acaagctggc
     3061 caagctttac ggcgtttcta ttgataccat tgtacacatg gaaggcgaag tacctaaaga
     3121 gattgttatc gaggataaga ccacaagcga acaaataagg cttatccagg aacttgacga
     3181 aaaggataaa ggcattattt tctctatgat tgatataatg ctcaccaaaa agaaacttaa
     3241 gcattttttc cagaaaaacg ttgctgcact actaactaaa acgcccggac ttgccgggcg
     3301 tttttactat ctcacaaagg ctatact
//
LOCUS       DAORPD010000068         3320 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_5081_length_3320_cov_2.411945, whole genome shotgun sequence.
ACCESSION   DAORPD010000068 DAORPD010000000
VERSION     DAORPD010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3320
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5081_length_3320_cov_2.411945"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..725
                     /locus_tag="PK976_01350"
     CDS             <1..725
                     /locus_tag="PK976_01350"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRC66565.1"
                     /translation="FLGGPGSSGVLIFDSSLYHNPVPDQPGGGTVDWTNPWGEYKYID
                     DIELREDGGTPGFLQAIRTALCIELKNRMGTPNIHQREQQLVKKAFELFRRIPHVHIL
                     ADEFEDRLGVFSFYIDNVHYNLVVKLLNDLAGIQVRGGCTCAGTYGHYLLNVSYEQSK
                     RITDKINQGDFSEKPGWVRLSLHPTMTNEELEIAAATTYEIATNIEYYREQYIYDPRK
                     NEFRHRSEPIDKIVFVKDWFTL"
     gene            888..2525
                     /locus_tag="PK976_01355"
     CDS             888..2525
                     /locus_tag="PK976_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRC66566.1"
                     /translation="MMKESYVGLVNESLETNWDLKAFSDYEGGTYSFAEVASHIHYLH
                     QLYQKCGIKAGDKIAIYGRNSSNWAIAYLSVITYEAIVVPILPNFGPNEVHHLMNHSE
                     SCFVFASQSLWEALDASKMLTIMMSISLDDFSCLYATQDTFLDNVEEFNRIKSNLQDK
                     TCINWQVPSNDKLLAIIYTSGTTGFSKGVMLTHNSITANIVFSRNHMLLKPGDRILSL
                     LPLAHAYGCSIEFLYPFTLGCHITFLGKTPSPQVLMRAFSEVKPRLILSVPLILEKIY
                     KTQVKATLSTPRMKIFLGIPFIENIVYKKVLQKLLDFFGDNFIELIIGGAALNEEVQI
                     FLRKIRFPFTVGYGTTECGPLITYAPWYENRLNSCGRCVDTLEMKIDSADPQKEVGEI
                     LVKGENVMVGYYKNPEATRETFTNDGWLRTGDLGIMDSDGFLYIKGRIKSLIVGPSGQ
                     NIYPEEIESIINNWPYVTESLVVERKNQLVALIYPNMEEADKDKIGENQLSESYNKLR
                     EEMNKKIPAYMNVSRIEIYPHEFEKTPKNSIKRFLYS"
     gene            complement(2555..3220)
                     /locus_tag="PK976_01360"
     CDS             complement(2555..3220)
                     /locus_tag="PK976_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005779953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HRC66567.1"
                     /translation="MSVSDNLKKILSTLPQGVKLVAVTKTHPPEVIMEAYQAGHNIFG
                     ENRAQEMRSKYEVLPKDIEWHFIGHLQSNKVKYIAKIANMIQSIDSLSLLKEVDYYAK
                     KNKRIASCLLQFHIATEETKFGLNWEEACKLLEDPSITQLKNISIEGVMGMASFTEDK
                     NLIRKEFKQLRMYFEELKQLYFFHQNSFCEISMGMTNDYHIAIEEGSTIVRIGSAIFG
                     SRL"
BASE COUNT          973 a          644 c          668 g         1035 t
ORIGIN      
        1 agtttcttgg tggtcccgga agcagcggtg tattgatttt cgattcatca ctctatcata
       61 accctgtccc cgatcagcct ggaggaggaa cagtggattg gacaaatcca tggggagaat
      121 ataaatatat cgacgacatc gaacttcgtg aagatggagg cactccaggt tttttacaag
      181 ccatccggac agctttgtgt atcgagctga aaaatcgaat gggaacccca aacattcatc
      241 aacgtgaaca acagctcgtt aaaaaggcct tcgaattatt tcgtcgaatc cctcatgttc
      301 atattctggc cgatgaattc gaagatcgac tgggcgtttt ttcgttttat atagataacg
      361 tacattacaa tttagtcgta aaactcttga acgatttggc tggaatacag gtaagaggtg
      421 gctgcacttg tgccggtaca tatggacatt atttattgaa tgtaagttac gaacaatcga
      481 aacgcattac cgataaaatc aatcagggcg atttttcgga aaaacctggc tgggtcaggc
      541 tttcacttca tcctacaatg acgaatgaag agctcgaaat tgcagcggct acaacctacg
      601 aaattgctac caatattgag tattatcgag agcaatacat ttatgatcca aggaaaaacg
      661 aatttcggca tcgttctgaa cccatagata aaattgtttt tgttaaggat tggtttactt
      721 tataaaattt ttcttcaaga ttttttcatt gataaagact tatttctatt ttttggtgta
      781 tctttattct ttggtctcta ttccaaattg aaagaattcc tgaagattac gcttattcga
      841 tcaaatactt tactttagca ttttactatt tctaaaatcc tatatccatg atgaaagaat
      901 catacgtcgg acttgtaaat gagagtcttg agaccaattg ggatctcaag gcctttagcg
      961 attatgaggg tggcacctat tcttttgccg aggttgccag tcatatccat tatttacatc
     1021 agctctatca aaaatgcgga ataaaagcag gggataaaat agccatttat ggtcggaatt
     1081 cgagcaactg ggcaattgct tatttgtcgg ttattaccta cgaagccata gtagtcccca
     1141 tactgcctaa ctttgggcct aacgaagttc atcatttgat gaatcattcg gagagttgct
     1201 tcgtattcgc ttcgcagtct ctatgggaag ctttggatgc ttcgaagatg cttacaataa
     1261 tgatgagtat ttctttagat gatttttcct gtctttatgc gactcaggat acatttctgg
     1321 ataatgtcga ggaattcaat cggataaaat cgaatctaca ggataaaaca tgtatcaact
     1381 ggcaggttcc atccaacgac aagcttctgg ccattattta cacttctgga accactggtt
     1441 tttcgaaggg ggtaatgctg acacacaata gcattacagc taatattgtt ttttcgagaa
     1501 atcacatgct cttgaagcca ggcgaccgta ttctgagctt attgccactg gcgcatgcat
     1561 acggatgttc tatcgaattt ttatacccct ttactctggg ttgtcatatc acctttcttg
     1621 ggaagacgcc atcacctcaa gtattgatgc gggctttttc cgaggttaaa ccccgtctta
     1681 ttctttctgt tcctctcata ctcgagaaga tttataagac tcaggtaaaa gcaacacttt
     1741 ctaccccacg catgaaaata tttctcggca ttcctttcat cgaaaacata gtgtataaga
     1801 aagtccttca gaaactcttg gattttttcg gtgataactt cattgaactc atcattggag
     1861 gtgcagcact taacgaggag gttcagatct tcttgaggaa gataagattt ccatttacgg
     1921 taggctatgg aactacggaa tgcggtccac tgattactta cgctccctgg tacgaaaatc
     1981 gccttaattc gtgtggtaga tgtgtggata ccttagaaat gaaaattgat tcagccgatc
     2041 ctcagaaaga agttggcgaa attttggtaa aaggagaaaa tgtgatggtg ggctattata
     2101 aaaatcctga ggctactcgg gaaactttta ccaatgatgg atggctgcgt acgggcgatc
     2161 tgggcatcat ggattccgat ggatttctct atatcaaggg ccgaatcaaa agcctgattg
     2221 ttggcccatc agggcaaaac atctatcccg aagaaattga atccattatc aataactggc
     2281 cttatgtaac cgaatcactg gttgtggaac ggaagaatca actggtagca ttgatttatc
     2341 caaatatgga agaagctgat aaggacaaga ttggtgaaaa ccagctgtct gaaagctaca
     2401 acaaactaag agaagagatg aacaaaaaga ttcctgcgta tatgaatgtg agccgcatcg
     2461 aaatctatcc tcatgaattc gaaaaaaccc caaaaaatag tatcaaacgt ttcctgtatt
     2521 cgtaacaatc ctcgatagtt agtatttatc gtgtttacaa cctcgaccca aatatagcag
     2581 agccaatacg tacaatagtg ctgccttctt cgatcgcaat gtggtaatcg ttggtcattc
     2641 ccatcgaaat ctcacaaaat gaattttgat gaaagaagta gagctgtttc aattcttcga
     2701 aatacatacg caattgtttg aattctttcc ttataagatt tttatcttcg gtaaaggatg
     2761 ccatacccat tactccttca atggaaatat ttttcaattg ggttatggac ggatcttcta
     2821 ataacttaca ggcttcttcc caattgagtc caaatttggt ttcctcagtg gcgatatgaa
     2881 attgcaataa acatgatgcg attcttttat tcttctttgc gtaataatca acctctttta
     2941 gaagggaaag actgtctatc gactgtatca tgtttgcaat cttcgcaatg tatttcactt
     3001 tattggattg caaatgtcct atgaaatgcc actcgatatc tttgggaagc acttcatact
     3061 ttgagcgcat ctcctgtgcc ctgttttctc caaagatatt atggcctgct tggtaagctt
     3121 ccattatcac ttcgggcgga tgagttttgg taactgctac gagcttaacc ccttgaggta
     3181 aagttgatag aatttttttt agattatcgg ataccgacat gacgaacact tttgaagaat
     3241 gaagtacaaa ggtatgaaag ccactcgaag tgatggaatt ttactcgtac gattggattc
     3301 tttctgggat tagcatttcc
//
LOCUS       DAORPD010000069         3311 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_5096_length_3311_cov_2.144963, whole genome shotgun sequence.
ACCESSION   DAORPD010000069 DAORPD010000000
VERSION     DAORPD010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5096_length_3311_cov_2.144963"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            404..2269
                     /locus_tag="PK976_01365"
     CDS             404..2269
                     /locus_tag="PK976_01365"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HRC66568.1"
                     /translation="MSLSRRKTSLIICLGFSYLLSVAQQPSIERIEPPFWWIGMNESN
                     LELLFYGKNLGDFKAYSSVPGLEIINTISIENPNYLFVYIKMDSNLKEGVYPLLLKGD
                     KDKEISVLYRFDKREPGSASRKGFGWGDNIYLLMPDRFANGNPSNDDIDAMVEKADRN
                     NPNGRHGGDLAGVAKYLDYLKELGMTAVWLNPIFENNQPHYSYHGYAITDLYNVDPRL
                     GSNEEFVRVVKQAHQKGLKIIMDMVFNHLGDQHWMIRDLPSSSFIHRWPEYTPSNFRS
                     TTIPDPYHSEYDYQKMINGWFDRHMPDLNQNDSLLADYLIQNSIWWIEYSGIDAIRMD
                     TYQYADKDFMARWVNRIKGEYPDFSIVGELWVEQKALHAYWMEGNYRFGNYDSPLQFL
                     TDFPLYIALRESFLDKEKGWEKGVERIYYCLAQDFVYDEPRNNMIFIDNHDLSRFYSI
                     AGENLQKFTLGLVMLYTLRGIPQFTYGTEQLMTGLESDGHGLIRGDMMGGWPNDPVNA
                     FEAKGRNKQQQKAWELISSLANWRKSSNVIHSGNTRHFLPENNVYSYFRYNENDTVWV
                     ILNYGDEEALVETGRFAEMLGQHRRGHEIVSNREISWGETLTLQPYQALIIELIP"
     gene            2288..>3311
                     /locus_tag="PK976_01370"
     CDS             2288..>3311
                     /locus_tag="PK976_01370"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC66569.1"
                     /translation="MKTMQKKLSNVFYVILGLPATAMGFGLSVQIAVLSWILRSKYGL
                     NIEEIGIVWAAGPIAGILGQPIVGLISDNVWFWGGRRRPFIIIGGILAALAIFALPHI
                     GFISHTLGMESIIGVAITVALTLDLSINISFNPTRSIIADVTPEGPQRTKGYTWMQAI
                     SNFFGALAYLIAALFGNYVLIYVGVVIILLFSIIPLFFITEPRHLEQSIIDNLSNTAS
                     TTKTQWGELFKIYFAHAFSWLGIQTMFVYSFAFLEQRFNVNDAQYLGQIIGWMFFILN
                     AVGSLFPIFFLEPLSRKIGRVKTHYLSLLTMSLGYFLIAFYVRSPQEIYAVFILLSLG
                     WAAVVSL"
BASE COUNT          947 a          620 c          715 g         1029 t
ORIGIN      
        1 ttatggaata tagaagccgc atcgagcagc ataaccgtag acaccagtgg aacatagagt
       61 attactgtaa tttctcaaaa tggctgtatg ggcagaggta atatcgatgt attcactggt
      121 caaggcacga aaccttcatc gaggctcatc atactcaatt gatttttatg gtacattggc
      181 taaattttca cgttgctgga atacttcgag aataacagta aaggacttat tgagtaaaag
      241 tcgaagattc tccattgact ttttcttttg agctgataat gaaaattttt ataaatattt
      301 gccttagatt tgcgtcgata aaaagaaaaa aatccacaga ccactcgatt gtttagagaa
      361 ttatatccat atcactcata aatgtaaatg aatgaagatc aggatgtcac tatcacgcag
      421 gaaaaccagc ttgattatct gtttggggtt ttcatactta ttaagtgttg cacaacagcc
      481 ctcgatcgaa agaattgaac ctccgttttg gtggatagga atgaatgagt cgaatctgga
      541 attattgttc tatgggaaaa atttgggaga tttcaaggct tattcgtcag tgccaggatt
      601 ggagattatt aatacaatat ctattgagaa tcccaattat ctttttgttt atataaaaat
      661 ggattccaat ctgaaggagg gtgtttatcc gcttttgttg aaaggtgaca aggataaaga
      721 gataagcgtt ttgtatcgtt tcgacaagcg tgaacctggt tcggcaagtc gaaaaggttt
      781 cggatggggc gataatattt atttacttat gcctgatcgt tttgcaaacg gaaatcccag
      841 taatgacgat atagatgcca tggtagaaaa agccgaccga aacaatccga atggtcgtca
      901 tggtggggat ctggctggtg tggccaaata tctcgattat ctgaaagaat tgggaatgac
      961 ggctgtttgg cttaatccga ttttcgaaaa caatcaacca cattattctt atcatggata
     1021 tgccatcact gacctgtata atgttgatcc gcgcttgggt tctaacgaag aattcgttcg
     1081 agtggtaaaa caggctcatc aaaaaggatt aaaaattatc atggatatgg tttttaatca
     1141 tttaggcgac caacactgga tgatacgtga tcttccttcc tcttcattca ttcaccgatg
     1201 gccagaatat accccttcga attttcgcag taccactatt cccgatcctt atcattcaga
     1261 atatgattat cagaaaatga taaatggttg gttcgatcgc cacatgcccg atctgaatca
     1321 aaatgattca cttcttgccg attatctgat acaaaatagt atctggtgga tcgaatattc
     1381 cggcattgat gcgatcagaa tggacactta tcaatatgcc gataaggatt ttatggccag
     1441 atgggttaat cggatcaagg gagaatatcc cgattttagt atagtgggtg agttatgggt
     1501 agaacaaaag gccttgcatg cttattggat ggaaggaaat tatcgtttcg gtaattatga
     1561 ttctcccctt cagtttttaa cagatttccc cttgtatatt gccttgaggg aatcattcct
     1621 cgataaagag aaagggtggg aaaaaggtgt ggaaagaatc tactattgtc ttgctcaaga
     1681 tttcgtatat gatgaacctc gtaataacat gatttttatt gataatcacg acctgagcag
     1741 attttattct atagcgggtg aaaatcttca gaaatttact ctcggactcg ttatgcttta
     1801 taccctaaga ggcattccac aatttaccta tggcactgaa caactgatga cgggtctgga
     1861 atcggacggc catggtctta tacggggaga catgatgggc ggctggccta atgaccctgt
     1921 taatgccttc gaagcaaaag gacggaataa acagcaacaa aaagcatggg aattgatcag
     1981 ctctctggct aattggcgga aatcatcaaa tgtaatacat tcaggaaaca caaggcattt
     2041 tttgcccgaa aacaatgtct attcctattt tcgctataac gaaaacgata cggtatgggt
     2101 tattttgaac tatggagatg aagaagccct tgtagaaacg ggcagatttg ccgaaatgtt
     2161 gggccaacat cgccgtgggc atgaaattgt ctcgaaccgc gaaataagct ggggagaaac
     2221 tttaacttta cagccctatc aagctctaat tatcgaactt atcccctaaa cctcaaatat
     2281 tcatcttatg aaaacgatgc agaaaaagct cagcaatgtt ttttatgtga ttttaggttt
     2341 gccagccaca gcaatgggat ttggcctttc ggttcagata gccgttttaa gctggatttt
     2401 acgaagcaaa tacggactca atattgagga aatcgggata gtgtgggccg caggacctat
     2461 tgccggtatt ttgggacaac ctattgtggg attgattagt gataacgtat ggttctgggg
     2521 tggaaggaga cgcccgttca tcattattgg aggtatcttg gcagccttgg ccatttttgc
     2581 ccttccccat atcggattca tcagccatac cttgggaatg gaaagcatta tcggtgtggc
     2641 catcaccgtg gctttgaccc tcgacttaag tatcaatatc agttttaatc ccacgcgttc
     2701 tatcattgcc gatgttacac ccgagggtcc ccaacgtact aagggatata cttggatgca
     2761 ggcaatttcg aatttcttcg gtgctctggc gtatcttatc gctgcactgt ttggcaatta
     2821 tgtgttgatt tatgtaggtg tggtgatcat attgttgttc tcgataatac ccttgttttt
     2881 tatcaccgaa cccagacacc ttgaacagtc catcattgat aatctttcaa atactgcatc
     2941 aacaactaaa acccaatggg gggaattgtt caagatttat ttcgcccatg ctttttcatg
     3001 gttgggcatt caaaccatgt tcgtctattc ttttgctttt ctcgaacagc gcttcaatgt
     3061 aaacgatgcg caatatcttg gacagatcat cgggtggatg ttttttattt tgaatgcagt
     3121 aggttctctt ttccctattt tctttcttga acctttatca cggaaaattg gtagagtaaa
     3181 aacccattat ctctctttgc ttaccatgtc gttaggctat ttcttgatag ctttctatgt
     3241 tcgttcgcct caggaaatat acgcagtgtt catattgctc agtcttggat gggctgccgt
     3301 agtaagcctg c
//
LOCUS       DAORPD010000070         3303 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_5121_length_3303_cov_2.179495, whole genome shotgun sequence.
ACCESSION   DAORPD010000070 DAORPD010000000
VERSION     DAORPD010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5121_length_3303_cov_2.179495"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(257..2197)
                     /gene="dnaG"
                     /locus_tag="PK976_01375"
     CDS             complement(257..2197)
                     /gene="dnaG"
                     /locus_tag="PK976_01375"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HRC66570.1"
                     /translation="MIPESTVSLILETARIEEVIGEYVHLRRRGANLVGLCPFHSEKT
                     PSFTVSPLKGFYKCFGCGAAGNVVKFLMEYEHYTYIEALRYLARKYNIEIEEREPTEE
                     EIQKANEKESLYSVNAMAAEFFKTQLWETPEGRNIGLSYFEERGFQHPIIQRFQLGYS
                     PMQKDALYIYALSKGFDPDYIEKAGLIIKHGNEYIDRFFNRVIFPIHNQSGRIIGFGG
                     RILTNDKLFPKYINTPETEIYHKSNVLYGFYQARKQIADANNCYLVEGYTDVISMNQI
                     GVSNVVASSGTSLTPGQIHLIKRYTPNVTILYDGDTAGIKASFRGIDMILQEGMNVRI
                     VLFPKDHDPDSFARSHRTEEVVSFITEKAQHFIGFKTNVLLDETQNDPIKRSALIKEI
                     LDSIALVPSSIARSEFIKYTSETLNIPEQTLLYELNKILSRKYKNFKPEPQPLSPPNL
                     QIDSNQEIAEERNITHQEYNIIRLLLNHGEKNFEFQFDDESGQQQFMKVNTSHFIVSE
                     LIEDDYEFSSPVLQKLYQEYKAAFEKNIILPEKYFINHPDEEIANIAMNFLASNYQIS
                     ENWFKQKGIFTPKEIDKLKDLVINSVYNLRLRRIELEINKVQQELQRADWEEAWNLLK
                     QLNDLNVSKSQISKKLGRIILK"
     gene            complement(2324..>3303)
                     /locus_tag="PK976_01380"
     CDS             complement(2324..>3303)
                     /locus_tag="PK976_01380"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRC66571.1"
                     /translation="KADKKKFAEIAAKFSDDPSVTQNSGDLGWFADGQMIYPFNQAVL
                     KTKVGDFTTVESAFGYHVIEVTGKTTPIKKVRVEILKKVINPSTRTQQKIYAQASTFA
                     AQCKTRDDFDKKVQELGLNKRSKDYLNAMDMGLPGIDDSRDIVRWAFSKEVKLGAISD
                     VKEFGNKFVVATLKKIHPKGIAPLEDIKEGIEASVKKEKKVQMLAAKMKEVQTKDLYE
                     LAIRLNAKVDTASDITFYTYNIPGIGREPNLIGTVFSLNPNEVSDPIEGINNAFVAKV
                     KSFTDAPAKSDFTFETKQLENNFFARSSSGTYKALEKLAEIEDNRVLFY"
BASE COUNT          929 a          624 c          607 g         1143 t
ORIGIN      
        1 aagtgatcca taagaattga tttaacccag cttgagcatt ttaccctcaa aaagttgaat
       61 taattatttg tatttacatg aggctatttt acaggaaaaa tttgtgttcc ctgaaagttg
      121 aaactcaaaa attcaacttg ctgtatacaa ggaaattgca tccctaaggc tctcaagttg
      181 ggttgggtta aagcgggcac atatttgtta caaattggtt tccacgataa aacttccatc
      241 caaataaagc cgatgattat ttgagtatga ttctcccaag tttcttcgag atctgagatt
      301 tcgaaacatt taggtcattg agctgcttta gcaaattcca ggcttcttcc caatctgctc
      361 gttgtaattc ttgctgtacc ttgtttattt cgagctctat gcgtcggagc cggagattat
      421 aaactgagtt gatgactaaa tcttttaact tgtctatttc tttaggtgtg aaaattccct
      481 tttgcttaaa ccaattttca ctgatctgat aattggatgc taaaaaattc atagctatat
      541 tagctatttc ctcatcagga tgattgataa aatatttttc aggcagtatg atattttttt
      601 cgaacgctgc tttgtattcc tggtaaagtt tttgaagaac tggtgagcta aattcataat
      661 catcctcgat tagttcactt acaatgaagt gagatgtatt cactttcata aattgttgct
      721 ggccactttc atcatcgaac tggaactcga aattcttttc tccatgattt aggagcaaac
      781 gaatgatgtt gtattcttga tgagtaatgt ttctttcttc agcaatttcc tgatttgaat
      841 ctatctgaag gtttggcggg gaaagaggtt gaggctcggg tttaaagttc ttgtattttc
      901 tcgaaagtat cttgttcagt tcatagagca gagtttgctc aggtatattt aaggtttccg
      961 aagtatattt gataaattct gatcttgcaa tgctactggg cactaaggcg atggaatcga
     1021 gaatttcttt aatcaaagcc gatcgtttta tgggatcatt ttgtgtttca tcgagcagaa
     1081 cattggtttt aaaacctata aagtgttgag ctttttcggt aataaaactt actacttctt
     1141 cggtacgatg gctgcgggca aaagaatcgg ggtcatggtc tttggggaaa aggacaattc
     1201 ttacattcat cccttcctgt aaaatcatat caatgccgcg aaatgatgct tttataccgg
     1261 ctgtatctcc atcatagagt atagtaacat ttggagtata tctcttgatc aaatgaattt
     1321 gtccaggagt gagtgatgtt cctgatgatg ccacaacatt gcttactcct atttgattca
     1381 ttgaaattac gtcggtatag ccttctacca gataacagtt atttgcatcg gcaatttgct
     1441 ttcgtgcctg ataaaatcca taaagaacgt tgcttttatg atagatttcc gtttcgggag
     1501 tattgatgta tttgggaaat aatttgtcat tggtgaggat tcggcctcca aaaccaatta
     1561 ttctacccga ttgattgtga atggggaaga ttacacggtt aaagaatcgg tcaatgtact
     1621 cattcccatg ttttataata agccccgctt tttctatgta atcaggatca aatcctttcg
     1681 aaagggcata tatataaagt gcatcctttt gcatgggaga atatcccagt tgaaatctct
     1741 gaataattgg atgctgaaag ccacgttctt cgaaatagga taatcctatg tttcttcctt
     1801 caggtgtttc ccataattga gttttaaaga attcagcggc catggcattc accgaataaa
     1861 ggctttcttt ctcgtttgcc ttttgaattt cttcttccgt tggttcacgt tcttcaattt
     1921 cgatattgta ctttcgcgcc aaatatctaa gtgcttctat ataagtataa tgctcatatt
     1981 ccataaggaa tttcacaaca tttcctgcag caccacatcc aaaacactta taaaagccct
     2041 tcaagggtga tacggtaaaa gaaggtgtct tttcgctatg aaatgggcac aatccaacca
     2101 gattggcccc tcttcgacgt aaatgaacat attcgccaat aacttcttct attcgggcgg
     2161 tttcaagaat cagacttact gtggactcgg gtatcaagat cgaaaaaaat ttttcaaaat
     2221 tattacgaat ggagcaaata gattaggtaa agttaaaaaa accagcttgt gtaaagctgg
     2281 tttctagaaa aaagaatttt ttccgcctct tgtggaaatt ccatcaatag aacaacacac
     2341 gattatcctc aatttctgcc agtttttcca gcgctttgta agttcctgag ctgctgcggg
     2401 caaaaaaatt gttttcgagt tgtttcgttt caaatgtgaa atctgacttt gccggagcat
     2461 cggtaaagga cttcacctta gcaacaaaag cattgttaat accttcgata ggatcggaga
     2521 cttcattggg attcagcgaa aaaactgttc caataagatt tggctctctt cctatgccag
     2581 gaatattata ggtgtaaaaa gttatatcag aagctgtatc gaccttggcg ttcaatctaa
     2641 ttgccaattc atacaggtct tttgtttgaa cttctttcat ttttgcagcc agcatttgaa
     2701 ctttcttttc ctttttcacg gatgcttcaa tgccttcttt gatatcttca agtggagcaa
     2761 tgcctttggg atgaattttt tttagtgtgg caactacgaa tttattgcca aattctttca
     2821 catcggaaat cgctcctagt tttacttctt tgctaaatgc ccagcgaacg atatctctcg
     2881 aatcatcgat accgggcaaa cccatatcca ttgcattcaa ataatccttc gaccgtttgt
     2941 tcaaccctaa ttcctgaact tttttatcga aatcatctct tgttttacat tgggcagcaa
     3001 aagtacttgc ttgagcataa atcttttgct gagtacgagt actcggatta atgactttct
     3061 tcaaaatttc tactcgtact ttcttaattg gagtcgtttt ccctgtaact tcaattacat
     3121 ggtaaccaaa cgccgattcc acagtagtaa aatcaccaac tttcgttttc aacactgctt
     3181 ggttgaatgg ataaatcatc tgaccatcgg caaaccagcc taaatctcct gaattttgtg
     3241 taacggatgg atcatcggag aattttgcag ctatttcggc aaattttttt ttatcggctt
     3301 tta
//
LOCUS       DAORPD010000071         3299 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_5130_length_3299_cov_2.210543, whole genome shotgun sequence.
ACCESSION   DAORPD010000071 DAORPD010000000
VERSION     DAORPD010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5130_length_3299_cov_2.210543"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1765
                     /gene="porU"
                     /locus_tag="PK976_01385"
     CDS             <1..1765
                     /gene="porU"
                     /locus_tag="PK976_01385"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HRC66572.1"
                     /translation="DKIENYFKEGPPAMLPWKNTFCFIADDENGNEHVKGAEKLANIV
                     DTSSSIINVNKIYIDAYKQYSTSNGDKYPDVNKAITDQVNEGALFINYTGHGGETGWA
                     DENILTVPEIFNWNNKGKMPIFITATCQFSRYDDPERVSAGEHVIQYNNKGGVALFTT
                     TRLTFSHTNNFLNQAFYVSLFEQLKSGNIPHMGDLIRMSKNKNNNNISIRNFSLLGDP
                     VLIPAFPRYSIKTTHINDIPIEYFIDTLRGCQKVKISGEIVDEQENILSNFNGKIFVS
                     VFDKKATIRTLGNDPNTTPMSFTIQNKRIAHTISKVINGRFEVWFILPKDIEQNYGFG
                     KFSYYATDSISDAAGAFIDFIIGGIDEVLATDTEGPEIEIFLDDKNFISGNLSSPNPV
                     LLASLADSSGINCFGYGLGRNISAWIDGQTSNAINLDDFFVPLDPLNTRGNITYTFHN
                     LENGQHTLTLRAWDLQGNSNQKTIKFTVAPNASIKLWKITNHPNPFSDKTFFYFEHNQ
                     PGNSLRITIHIFNFQGIKVKTIRSKQSTSGYSIIPIEWDGRGDSGELLPSGMYPYQII
                     VENNNGTISTQSQKLMIIRHN"
     gene            1897..3087
                     /gene="porV"
                     /locus_tag="PK976_01390"
     CDS             1897..3087
                     /gene="porV"
                     /locus_tag="PK976_01390"
                     /inference="COORDINATES: protein motif:HMM:NF033710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system outer membrane channel
                     protein PorV"
                     /protein_id="HRC66573.1"
                     /translation="MPKFIKPTALALFTGLMCIPAILKAQDEYPITTAVPFLLIGPDA
                     RSGSMGETGVATDPDPVSIHWNAAKYAFIDKQMGFSVSYSPWLRQLVNDIGLGYLTFY
                     YRFDDKQALGASLRYFSLGEITFTDYIGNPKGTYKPNEFSLDAAYSRKLTDNLSGAVT
                     GRFIYSNLTLGQNVQGVESHAGTSFAADISLFYTKDLYINRNDAWFNFGLNISNIGAK
                     IAYTSTIEKDFIPTNLRFGPSFKYAFDDYNTLSFNIDITKLLVPTPPIYKLDSISGQP
                     IFDEQGNQVIEKGYNPNVSVFKGMIQSFYDAPGGFSEELKEITYAIGAEYLYNKQFAI
                     RAGYFHESATKGARQFFTFGAGLRYNVFSIDFSYLVSTDQRNPLDKTLRFTLNFDFEA
                     FRSH"
BASE COUNT         1030 a          712 c          609 g          948 t
ORIGIN      
        1 agataagatt gaaaattact tcaaagaagg tcctcccgcc atgctcccat ggaagaacac
       61 gttctgcttt atagccgacg atgagaatgg aaacgaacat gtaaagggag cagaaaagct
      121 tgctaatatc gtagatactt cttcttcgat aattaacgtt aacaaaattt atatagatgc
      181 ttacaaacag tatagcacct ctaatggcga caaatatccc gatgtcaaca aggctattac
      241 cgatcaggtg aacgaaggag ctctgtttat caactatacc ggtcatggag gagaaaccgg
      301 atgggcagat gaaaatattc ttacagtgcc cgaaattttt aattggaaca acaaggggaa
      361 aatgcccatc tttattactg ctacttgcca gtttagtcgc tatgatgatc cggagcgggt
      421 ttcggctggc gaacatgtca tacaatacaa caacaaaggt ggcgtcgcct tgtttacaac
      481 tacacgtcta acattttcac atacaaacaa tttcttgaat caggccttct atgtctcgtt
      541 atttgaacag ctcaagtcag gcaatatacc tcatatggga gacctcatcc ggatgtcgaa
      601 gaacaaaaac aacaacaata tatcgatccg aaatttcagt ttattgggag accctgtact
      661 tataccggcc tttcctcgat attctatcaa aacgacacat atcaatgata ttcccatcga
      721 atatttcatc gataccttga gaggttgcca aaaagtgaaa ataagcggag aaattgtaga
      781 tgaacaagaa aatattcttt caaacttcaa cggaaagatt ttcgtctcgg tcttcgataa
      841 gaaagccact attcggacgc ttgggaacga tccgaatact acacccatgt cttttacgat
      901 ccagaataag cgaattgctc ataccatctc taaagtaata aatggaagat tcgaagtttg
      961 gtttattcta ccaaaagata tagaacagaa ttatgggttt ggaaaattca gctattatgc
     1021 cacggatagt attagtgatg cagctggagc atttattgat ttcatcatag gcggaataga
     1081 cgaagtcttg gctaccgata ctgaaggacc cgaaatcgaa atatttctcg acgacaagaa
     1141 ctttatttcg ggaaacctct cgagccctaa tcctgttctg ctggcttctc tggccgattc
     1201 ttccgggatt aactgtttcg gttatggatt aggacgcaac atctctgcat ggatagatgg
     1261 acaaaccagt aatgccatca atctcgacga tttcttcgtc ccactcgacc cattgaatac
     1321 tcgcggaaat atcacctata ctttccataa cctcgaaaat ggacagcaca cacttacgct
     1381 gcgggcctgg gatcttcagg gaaatagcaa ccaaaaaacc ataaaattta ccgtagcacc
     1441 caacgcttct atcaaattat ggaaaatcac caatcatccc aatcctttca gtgataaaac
     1501 cttcttttat ttcgaacaca atcaaccggg aaattccctg cggataacta ttcatatctt
     1561 caacttccag ggaatcaagg taaaaaccat aaggtcgaag caatctactt cgggttatag
     1621 tataatcccc atcgagtggg atggaagagg cgattcggga gaattacttc catcgggaat
     1681 gtatccttat cagattattg tagagaataa taatggaact ataagtacgc aaagccaaaa
     1741 gttaatgatt atcaggcata attagggaaa aaccaattct gaatcaatcc tccaattctt
     1801 aatcccttgt gaatgaaaaa tactataaca tatcttttct cgttactttt gcagctttat
     1861 tttcaattca tcgaaatata aaacaaaata ccttatatgc cgaaattcat taaaccgact
     1921 gccttggctc tgtttacagg gttgatgtgt attcctgcaa tattgaaagc tcaggatgag
     1981 tatccgatta ctactgccgt accttttctt ttaataggac ccgatgcgcg ttctggttcg
     2041 atgggtgaaa cgggcgttgc aaccgatccc gaccctgttt ctattcattg gaatgcagct
     2101 aaatatgcct tcatcgacaa acaaatggga ttttcggttt cctattcccc atggctacgt
     2161 caattggtta acgatattgg actcggttac ctcacatttt attatcgctt tgatgataaa
     2221 caggccttag gagcctcact tcgctatttt tctctgggag aaatcacatt taccgattat
     2281 ataggtaatc ccaagggcac atataagccc aatgaatttt cgctcgatgc tgcttattca
     2341 cgtaaattaa ccgataacct ctctggagcg gtaactggcc ggttcattta ttcgaatcta
     2401 accttaggcc aaaatgtaca gggagtggaa tcgcatgcag gaacaagttt tgccgctgat
     2461 atatccttgt tttacactaa agacctgtat atcaaccgca atgacgcatg gttcaatttc
     2521 ggactaaata tttccaacat tggagctaaa atagcctata cctcgactat cgaaaaagac
     2581 ttcattccaa ccaatctgcg cttcggacca agttttaaat atgctttcga cgattacaat
     2641 accctctcct tcaacataga tattacaaaa cttctggttc ctacgcctcc catctacaaa
     2701 ctcgacagca tttcgggtca acccatattc gacgaacaag gaaatcaggt gatcgaaaaa
     2761 ggttacaacc ctaatgtatc tgtcttcaaa ggaatgatcc aatcctttta tgatgctcca
     2821 ggtgggttta gcgaagaatt gaaagaaata acttatgcta ttggtgctga atatttgtac
     2881 aacaagcaat ttgctatacg agcgggctat tttcatgaat ccgccaccaa gggagctcgc
     2941 cagttcttca ccttcggcgc tggcttacgt tacaacgttt ttagcatcga tttctcctat
     3001 cttgtgagca ctgaccaaag aaatcctctc gataaaactc tgcgtttcac tcttaatttc
     3061 gacttcgaag cattccgttc gcattgagat aattaactcg atccgaacaa gggttcgtta
     3121 aactcataat acaactcgcc ggttcaagac aaggaaccgg ttttttcata ataaccagaa
     3181 aatctattcg actttagttt caggggggat aattttttta taaagtcgaa aatttgaata
     3241 caaaaagaat tatttggaaa agggcttttc ctcctcgagt ttttgtaaat ttgaaacat
//
LOCUS       DAORPD010000072         3286 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_5152_length_3286_cov_1.856701, whole genome shotgun sequence.
ACCESSION   DAORPD010000072 DAORPD010000000
VERSION     DAORPD010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5152_length_3286_cov_1.856701"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1917
                     /locus_tag="PK976_01395"
     CDS             <1..1917
                     /locus_tag="PK976_01395"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRC66574.1"
                     /translation="VVPEEEIHAMPKLSVDEILAELPGVVVSRGSGILSSSATVSMRG
                     MGSEQGRTLVLLDGIPLNKADGGSVQWNNIDLNSIKQVEVLKGPGSSYFGGNAMGGII
                     SMVTAMPQKKIEGQAALEYGTYNSMGAHLNVGGKTGKFSWNAAGRGRKSDGYIVQPPD
                     EIDSTVIKTNMEEIGVNLKARYDIDTKQYVQVSYSYFDDKRGGGNNFHPFNEMAEPDG
                     TYTSHTNNYINVNYGFRADKILADVRAYYNDEDYVTISESLKGTQFTKYDVDSKRTDM
                     GLMSSATLPFQNGHSLTFGLDLKSGKVDGNDIYKTSTDKVINQGTNFNSGLFLMGNIQ
                     LLKGKLMLTPGLRWDYAHFYDGVFVVENPTKVTAFLVGFQDTMPTADWNAFSPRLGLQ
                     YNFNPDTRIYAQASSGFRPPILDDLCRTGRMRLGLKLANPNLKPERIYNFELGADYRY
                     KLITLSLSGYYSRGTDFIYYLATGDSAVVSNKLRPVLIKDNISDVDIYGLEFITKLQL
                     PAGFNLKANCAYTSTEIGNYIVKFPGREEDLTGKELVMVPKFQVGAGLYYSYKFVNAA
                     IVYRFTGKQWIDDSNKEELQETNLFDANLTLHLHRYIDASVAVYNIFDRLYYDTKGLM
                     TPGRFIMGRLTFKL"
     gene            1956..2975
                     /locus_tag="PK976_01400"
     CDS             1956..2975
                     /locus_tag="PK976_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66575.1"
                     /translation="MNKTIYSSFILLFFICMQNFCVFSQTNDFYDDQPTIPLGDVQIK
                     VEGEIEQHYHVDFTKLPLRSVIVKEAVLDSEGDHFIGAYRYDGYSLYDILNEAVLKKA
                     NQAEFPPVIDLFVEVEGVNGDKAIFSWGEIYYPIHRHEIIIATQISRIVPSKTKDYWP
                     LPGSPRVVAAADLHSHRYIENPKSIKVRSWNRPFVKHEEAKFTNKISLEIDGEKKMEI
                     VPSSLKPPISKYSTVFYGRGRGIHGISTFEGKPLKNLLSTFFKINPQILMEGLLIVYG
                     IDGYRSVFSCAEVMDRNDQEEILLVFNDNLDNPEAAGFRIFPACDFFSDRAVKAIEKI
                     EVVFK"
BASE COUNT         1020 a          659 c          681 g          926 t
ORIGIN      
        1 gttgttcccg aagaagaaat tcatgccatg ccaaaattga gtgttgacga gattttagca
       61 gaacttccgg gcgtagtggt ttcgagaggt tcaggaatct tatcatccag tgctacagtt
      121 tcgatgcggg gaatgggaag cgagcaaggg cgtacactgg ttttactcga tggaattcca
      181 ttaaataaag ccgatggagg ttcagttcag tggaacaaca tcgatttgaa ttccataaaa
      241 caggtcgaag tcttgaaggg acccggttca tcttattttg gtggtaatgc aatggggggc
      301 attatcagta tggtaaccgc catgcctcaa aagaaaatag aaggacaagc cgctctggaa
      361 tatggtacgt ataacagcat gggtgcacat ttgaacgttg gaggcaagac agggaaattt
      421 tcgtggaatg ccgcaggtcg tggccgtaaa agcgatggtt acatcgttca acctcccgat
      481 gaaatagatt caaccgtcat aaaaaccaat atggaagaaa ttggggtaaa cctgaaggct
      541 cgatacgata ttgacacaaa acagtatgtt caggtgtcgt attcttattt tgacgacaag
      601 agaggaggag gcaataattt ccatcccttc aatgaaatgg ccgaacccga cggaacctat
      661 acttctcata ccaacaatta cataaatgta aattatggtt tcagagctga taaaatcctt
      721 gccgatgtca gagcatatta taatgacgaa gattatgtta ccatttcgga aagccttaag
      781 ggaactcaat ttaccaaata cgatgtggac tcgaaaagaa cggatatggg cctcatgtcg
      841 tcggccaccc tgcccttcca gaacggccat tcgctcactt tcggtttaga cctgaaatcg
      901 ggaaaagtag atggcaacga tatatacaag acttcaaccg ataaggtaat taatcaggga
      961 accaacttta atagtggttt gtttttaatg gggaatattc aactcttaaa gggaaaattg
     1021 atgttaacac ctggcttgcg atgggattat gcccattttt acgatggcgt attcgttgtc
     1081 gaaaacccaa ccaaagttac agcatttctc gtcgggtttc aagacactat gcctacggca
     1141 gattggaatg cctttagccc aagattgggt ttacagtata atttcaatcc cgacacaaga
     1201 atatatgctc aggcatcgag tggtttcaga cctccaattc tcgacgacct ttgccgtacc
     1261 ggccgaatga gactcggact gaaactggcc aatcccaacc tgaaacccga gcgtatttac
     1321 aacttcgaat taggagccga ctatcgttat aaattgatta ctctctccct ttcgggatat
     1381 tattcgagag gaaccgactt catttattat ctggctaccg gcgactcggc cgttgtcagc
     1441 aacaaattac gacctgtttt gattaaggac aacatcagcg acgttgacat ttatggtctc
     1501 gagttcatca ccaaattgca acttccggct ggtttcaatt tgaaagccaa ttgtgcctat
     1561 acttccaccg aaattggaaa ttatatcgta aaatttcccg gtcgcgaaga agaccttaca
     1621 ggaaaagaac tcgtcatggt accaaaattc caagtcgggg caggtttgta ttacagttac
     1681 aaatttgtca atgcagccat cgtttatcgc tttacaggga aacaatggat cgacgacagc
     1741 aacaaggaag aattacagga aaccaacttg ttcgatgcta acttgactct tcatcttcat
     1801 cgctacatag atgcaagcgt agcggtttat aacattttcg accgacttta ttacgataca
     1861 aaaggattga tgactcccgg tagatttatc atgggaagat taaccttcaa attatagtta
     1921 attaaaatca ttgcaaaatc aatcatctga aaaatatgaa caagacaatt tattcgagct
     1981 ttattctctt gtttttcatt tgcatgcaaa acttttgtgt tttcagccaa accaacgatt
     2041 tctatgacga ccagcccacg attcctttgg gcgatgttca aataaaagta gaaggtgaaa
     2101 tagagcaaca ctaccatgtc gatttcacta aactgccact tcgcagtgta atcgtgaaag
     2161 aagccgtttt agactctgaa ggcgaccatt ttatcggagc ataccgttat gatggttatt
     2221 ctctttatga catcctgaac gaagctgttc taaaaaaggc gaatcaggca gaatttcctc
     2281 ccgtcatcga tctgtttgtg gaagtggaag gagtgaatgg tgacaaggcc atttttagtt
     2341 ggggcgagat atattatccc attcaccgac atgaaatcat tattgctaca cagatttcac
     2401 gaattgttcc gtcaaaaaca aaggattact ggcctttacc tggttctcca cgggtagttg
     2461 ctgctgccga tttgcattct catcgttaca ttgaaaaccc caaaagcata aaggtacgtt
     2521 cgtggaatcg accttttgtt aaacatgagg aagcaaaatt taccaataaa atttcccttg
     2581 aaatcgatgg agaaaagaag atggaaatag ttccctcgag cctgaaacca ccaataagta
     2641 aatattctac tgttttttac ggcagaggaa gaggaattca cggcatatcc actttcgaag
     2701 ggaaaccatt aaaaaatttg ctttctacct tctttaagat taatccacag atacttatgg
     2761 aaggattact gatagtgtat ggcatcgacg gctaccgaag cgttttctcc tgtgctgagg
     2821 tgatggatag aaacgatcag gaagaaattt tgctcgtttt taacgacaac ctcgataacc
     2881 ctgaagcggc cggcttcaga atcttccctg cctgcgactt tttcagcgat cgtgcagtga
     2941 aggctatcga aaaaatcgaa gttgttttta aataagaatt caactcttga aagaaaaata
     3001 tcggattttt taaaatgaat ttctcaaaac tccattaaat acaaggcgaa tagaagaacc
     3061 caacctctga acatctgaac atttgaatga tgcaaagtgg tgtatagcct gggctttggc
     3121 tcaggctttt ttaatctttt actaagacta ttattttatt ttagccgtca actaagcaat
     3181 gtattcaata ttcgaataag tttactccat tatcgctcta tccgatgtaa ttttgcattt
     3241 ataaaaaatt ctttttcatg gcaatagaaa acgaaaagtc aaaacg
//
LOCUS       DAORPD010000073         3259 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_5205_length_3259_cov_2.375156, whole genome shotgun sequence.
ACCESSION   DAORPD010000073 DAORPD010000000
VERSION     DAORPD010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5205_length_3259_cov_2.375156"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(451..1908)
                     /locus_tag="PK976_01405"
     CDS             complement(451..1908)
                     /locus_tag="PK976_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HRC66576.1"
                     /translation="MDQSLTSLQPQNVWHYFEEICKIPRPSKNEAQTRNYLLEFAKLH
                     HLEANTDDAGNVVIRKEAKPGFEDKPIVILQSHMDMVCEKNQDSLHNFEIDAIQPYID
                     GEWVKARNTTLGADDGIGMAAQLAILESDDIPHGSIECLFTVDEETGLTGASAIQPGF
                     LKGRILINLDSEDEGELFIGCAGGIDTLAELQFKRRVVPVNYLPYRIKVTGLKGGHSG
                     DDIHKGRGNANKILNRFLWNASNRYNIRLAQIDGGNLRNAIPREAEAVILVPFKTENM
                     LLEYFKEFYTEMVEELKYTEPHFCLTLESTTHPEAIINKKSQQRLLNAIYACPHGVIS
                     MSQRMPGMVETSTNLASIKITGPNTLAVNTSQRSDLESAKTDIASMVESVFLLAKFKV
                     THSDGYPGWTPNPSSRILNTTIQSYKNLFGNEPRVRSIHAGLECGLFLKKYPYLDMVS
                     FGPTLRDVHSPDEKIHIPSVDKFWKLILETLKNVS"
     gene            complement(2288..>3259)
                     /locus_tag="PK976_01410"
     CDS             complement(2288..>3259)
                     /locus_tag="PK976_01410"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HRC66577.1"
                     /translation="GMANIRYTDQFEATLKDLMVLTSNVFIDSNEYPKFFPEVKVNEH
                     RFALKMRKMFPLHTFSRLAPLLWKLRLNKEPEEIEFIRRASQLTGNCFRKILKITSPG
                     MFEHEIEAEITYEFARNGCCHAFQPTVASGANACYLHYRSHQQKLEKGDLLLLDFGAE
                     YKNYASDCSRTIPISGKFTPRQLQVYEAVLRLQRHALSMLVPGNTINQVNDELNHLAE
                     KEMINLGLFSEDDLLHQDPQNPLRTKFFMHGVSHFVGLDVHDVGSRDEVFSPGMILTF
                     EPGLYIPEENIGIRLENTFVVGYPSYNLMEDIPIDPDEIEELMIKPK"
BASE COUNT          885 a          574 c          706 g         1094 t
ORIGIN      
        1 tcccttttac tttgggggtg caaatttaca tcttttttta atatcacaag tgctctatta
       61 aaaatttttt taattcccct ctcaaaaggc taaaatgaac gtctcagttt tgagggtgca
      121 aagttaaaaa cattttaaaa caaaccaatg ttcatttata aaaaatttag aagatttctg
      181 ttagttctat ggatttgaac aatttgaaat cgaaaaaaat tttactcgtt caatcatttt
      241 ttccttaaat ggctttaata tggattacat tcgtagtttt tgtgatgtta tttgtttaat
      301 tcatagttgg atacaactta tctcttacta agttcgtttt gaggaggagt ggagaaatca
      361 ttctattatg ttcctgtaag cttgtaatta cttgattttg ttttgagagg ctgaagttag
      421 gagggtagaa ttacttcttt aaggtgttta ttatgaaacg tttttcaagg tctcgagtat
      481 caatttccag aatttatcaa ccgacgggat atggattttt tcatcgggtg aatggacatc
      541 acgcagggta ggcccaaagg aaaccatgtc gaggtaagga tattttttca ggaacaaacc
      601 acattcgagg ccagcgtgta tagagcggac ccttggttca tttccgaaga gatttttata
      661 agattggatg gttgtgttca gaattctgct cgaggggttg ggtgtccagc caggatagcc
      721 atcggaatgg gttactttaa atttagctag taggaaaaca ctttcgacca tggaagcgat
      781 gtcggtttta gcgctttcca gatcacttct ttggctcgta ttaactgcca atgtattagg
      841 ccctgtaatt tttatggatg caaggtttgt cgaggtttca accattccgg gcattcgttg
      901 gctcatgctt attacaccgt gtgggcaggc ataaattgca ttgagcaaac gctgttgcga
      961 ctttttattg atgatggctt cggggtgggt tgtgctttct agggtcaagc aaaaatgggg
     1021 ttcggtgtat ttgagttctt cgaccatttc ggtatagaat tctttaaagt actctaaaag
     1081 catgttttcg gttttgaagg gtaccagaat tactgcttct gcttctcgtg ggatggcgtt
     1141 acggagattt ccaccatcaa tttgcgccaa ccggatatta tatcgattgc tggcattcca
     1201 gaggaaacgg ttgagaattt tattggcgtt tcctcttcct ttatggatat cgtcgcccga
     1261 gtgccctcct tttagtcctg taacctttat tctataaggt aaataattaa caggaaccac
     1321 tcttcttttg aattggagtt cggccagagt atctatacct ccagcacagc caatgaataa
     1381 ttctccttca tcttctgaat cgagatttat aaggatgcgc cctttcagaa acccaggttg
     1441 tatggctgat gctcctgtta atcctgtttc ttcatccacc gtaaacagac attcgataga
     1501 accatgcgga atatcatccg attccaaaat tgccagttga gcggccatac ctattccatc
     1561 gtcggctccc aaggttgtgt ttcttgcttt tacccattca ccgtcgatgt aaggttgtat
     1621 ggcgtctatt tcaaaattat gaagtgaatc ttggttcttt tcgcatacca tatccatgtg
     1681 gctttgaaga attactatcg gtttatcttc gaaaccaggc ttggcttctt tgcgaataac
     1741 cacgttccct gcatcatcgg tattggcttc aaggtgatga agtttagcaa attccaacaa
     1801 gtagtttcgt gtctgagctt cgtttttcga tggcctggga attttacaaa tttcttcgaa
     1861 ataatgccag acattttgtg gttgaagcga tgtcaatgat tgatccataa gatatttatt
     1921 atatttagag ttaatgagac aaggtttatg attctaatga tttattattt atgacacgag
     1981 agcataactt gtttgttgaa tgtttcaaac actgatatat gggttttagt attaagaccg
     2041 ggtacattgt gtcaggcgac aaaataaaga aaaattttcg tgtatttctt tctcaacttt
     2101 attcatgtca aggattcgtt ttttaggaac gaatttttga gatgaaggga ggtattttga
     2161 taaaacaggt gtaataagtc ggaatttgga tgaaatttca agtgaagccg cttttactgc
     2221 acccttctga tgtgtgttta ctggatgaac tatcaaacaa agaaatgctc attatttcag
     2281 tatggtttta tttaggtttg atcatcaatt cttcgatttc gtccggatcg ataggaatat
     2341 cctccataag gttataagat ggataaccga ctacaaaagt attttcaagt cgaatgccaa
     2401 tgttttcttc tggaatgtat aaacccggtt caaaagtaag gatcatgcca ggggagaata
     2461 cttcatcgcg tgaacccacg tcgtgaacat cgaggcctac gaaatggctt acaccgtgca
     2521 tgaaaaattt ggttcgcaaa ggattttggg ggtcttgatg taagaggtca tcttcgctaa
     2581 agaggccaag gttgatcatt tctttttcag ccaagtgatt gagttcgtca tttacctgat
     2641 tgatggtatt acccggtacg agcatgctta gtgcatgcct ttgcagtcga agaacagcct
     2701 cataaacctg aagctggcgt ggagtaaatt tgccactgat ggggatagtg cgtgaacaat
     2761 cggatgcata atttttgtat tctgcaccaa aatctaagag caataaatcc cctttttcta
     2821 atttttgctg atgtgaacga taatgcaaat aacaggcatt ggctccagaa gcaacggttg
     2881 gttgaaacgc atgacaacat ccgtttcggg caaattcgta agtgatttcc gcttcgattt
     2941 catgttcgaa cattccgggg ctggtaattt ttagaatttt tctgaaacag ttaccggtta
     3001 attgggaagc tcgtcggatg aattcaattt cttcgggttc cttattgagg cgtaatttcc
     3061 aaagcaaagg agcaaggcgt gaaaaagtat gcaaggggaa catttttcgc attttcaaag
     3121 caaaacgatg ttcatttacc tttacctcgg gaaagaattt aggatattcg ttcgaatcga
     3181 tgaatacatt ggaggtcaga accatgaggt cttttagagt ggcttcgaac tggtcggtgt
     3241 agcgaatgtt tgccatacc
//
LOCUS       DAORPD010000074         3246 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_5231_length_3246_cov_1.757756, whole genome shotgun sequence.
ACCESSION   DAORPD010000074 DAORPD010000000
VERSION     DAORPD010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5231_length_3246_cov_1.757756"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(307..1407)
                     /locus_tag="PK976_01415"
     CDS             complement(307..1407)
                     /locus_tag="PK976_01415"
                     /inference="COORDINATES: protein motif:HMM:NF019365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015926 - glucosidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HRC66578.1"
                     /translation="MMKRFYKNIFYSIMILLFFLLPACSKESNPQPEPEPDAPSFFIR
                     GADLSLLPDIEKYGIQLYDINGNISDMLSIIKAAGCNTVRMRLWYNPADENSSFEQVK
                     DFAARVKAKGMDVWLCIHYSDTWADPGNQKTPRAWNYLHLAELKDSVYQYTYRVVSAI
                     QPLYVQTGNEVNDGFLWPLGRISTGGSFYELMTEAVKAAKAAAPAAHLMIHYAGIDGV
                     ALFFNQLKYKNANFDVIGLSYYPYYHGKDMSYLTSVMNSLSKNIQRPIIIAETSYPFT
                     LQWNDLSHNLIGTADQLIDSFPASPEGQRAYMIRLRHIMEGVKLGSGFCYWGGEWVAY
                     KGPDATDGSPWENQALFDFEMKALPVLDVFKK"
     gene            complement(1498..2748)
                     /gene="hutI"
                     /locus_tag="PK976_01420"
     CDS             complement(1498..2748)
                     /gene="hutI"
                     /locus_tag="PK976_01420"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HRC66579.1"
                     /translation="MIIITNIQQLVNVDESGRAVRVGKDMQKLNTISNAWLLIEGDEI
                     RGYGEMDSSEFQQFVNLPDIEVIDADKGMVFPSFCDSHTHLVYAGSREQEFVDKIRGL
                     SYEEIARRGGGILNSSRLLHETSEEQLLEQALFRLDEIIHQGTGAVEIKSGYGLNTED
                     ELKILRVIRRLKELSPLTIKSTFLGAHAIPLEYKDTPDDYVDVIIQEMIPQVASEDLA
                     DFIDVFCDRGFFTPEQTDRILMAGMKYGLKAKIHANELDLSGGVQVGVKYNALSVDHL
                     ENVGDEEIECLLQSETMPTILPGAAFFLNMPHAPARKMIDAGLPVAFASDYNPGSSPS
                     GNMQLVLSMACIQYRLLPEEAILATTLNSAYAMGVEDILGSIAKGKKANLFITHPIPS
                     IYFMPYAFGTNKVKHVILNGKKIF"
BASE COUNT         1023 a          724 c          556 g          943 t
ORIGIN      
        1 agttaaaaat ggagaaatga aagatggaga ttagttaacc atcaatatta agcataaaaa
       61 ttaattatga attaattgca caaaatctcc gaattgatat ttataaacct cggtagaaca
      121 aaatagatga aaatccccct cttaaacctt gtaacctttt ctattcttat ataaatacct
      181 gatataaaac agtttataaa attgtaagta gaacaggtgt aaatagaaat taattttcct
      241 caattttctg aaaataactt tccacacttt tgtaaaaagg tcaataaaca atgcacttgg
      301 ttctatttat ttcttgaata catcaagcac gggcagagct ttcatctcga aatcaaataa
      361 agcttgattt tcccatggag aaccatcggt agcatcgggt cctttgtaag ccacccactc
      421 tcccccccaa taacaaaaac cagaccccag cttcactcct tccataatat gacgaagcct
      481 tatcatgtaa gccctttgac cttcgggact tgccggaaaa gaatcgatca actgatcggc
      541 agtacctatc agattgtgag aaaggtcatt ccattgcagt gtaaatggat aagaagtctc
      601 ggcaatgatg atcggccgct gaatattttt actcaaggaa ttcatcacag aagtcaagta
      661 actcatatct ttaccatgat aataagggta atacgaaagg ccaatgacat caaaattcgc
      721 attcttgtat ttcaattgat tgaaaaacaa agctactccg tcgatacccg cataatgaat
      781 catcagatgt gcagcgggtg cagccgcctt tgctgccttt acagcctcag tcatcaattc
      841 atagaatgaa ccgccagtgc ttattcttcc taatggccac aagaaaccat cattcacttc
      901 attgccggtt tgaacatata atggttgaat ggcacttact actctgtaag tgtattgata
      961 aacgctatcc tttaattcag cgaggtgtag ataattccaa gctcgaggcg ttttttgatt
     1021 gccgggatcg gcccaagtat ccgaataatg aatgcacaac caaacatcca tacctttcgc
     1081 cttgaccctt gcagcaaaat cctttacctg ttcgaatgaa gagttttcat ccgccggatt
     1141 gtaccacaaa cgcatcctaa cagtattgca gcctgcagcc tttattatac ttagcatatc
     1201 agaaatattg ccgtttatat cgtaaagctg aattccatat ttttcgatat cgggaagcag
     1261 ggataaatcg gctccacgga taaagaagga aggtgcatcg ggttctggct cgggctgcgg
     1321 attagattct ttggaacagg ccggtaaaag aaaaaaaagc aaaatcatga tcgaataaaa
     1381 tatattcttg taaaatctct tcatcatctt ttttaaatgg tcaattataa cacatatcgc
     1441 tttcttaaca caaattaact catgaagcaa accccaaatt tcgagggatt ggtttagtta
     1501 gaaaattttc tttccattca ggatcacatg tttcacctta ttagttccga aggcataagg
     1561 cataaaatag atagaaggga tgggatgagt aatgaaaaga ttggcttttt tcccttttgc
     1621 aatggagccc aagatatctt ctacacccat ggcataggcc gaattgaggg tagttgccag
     1681 aatagcttcc tcgggcaata aacgatactg aatgcaagcc atagaaagaa ccagctgcat
     1741 attccccgat ggcgatgatc ccggattata gtcgctggca aatgcgacgg gcaggcctgc
     1801 gtctatcatt ttacgggcag gagcatgagg catattcaaa aaaaaggcag ccccaggaag
     1861 tatcgtcggc atcgtctcgc tctgaagcaa acactcgatt tcctcatctc caacattctc
     1921 gagatgatcg accgaaagcg cattgtattt cactcctact tgcacccctc ccgataaatc
     1981 gagctcattg gcatgtatct tcgccttcag accatatttc atacccgcca tcaaaatgcg
     2041 gtcggtctgt tcaggagtga aaaatcccct atcacagaaa acatctataa aatcggcaag
     2101 gtcttctgaa gctacctgag gaatcatctc ctggataatg acatcgacgt aatcatcggg
     2161 ggtatcttta tattccaaag gaatagcatg agcacctaag aaagtggatt ttatagtaag
     2221 agggctcaat tctttcaaac gtcggattac acgtaaaatc ttcaattcat cttcggtatt
     2281 caatccataa ccgcttttta tttcaacagc accagtacct tgatgaataa tctcatccaa
     2341 acgaaataga gcttgctcca acaattgttc ttcgctagtt tcgtgcaaca accgagacga
     2401 attcaaaatt ccccctcccc ttctggcaat ctcctcatac gaaagaccac gtatcttgtc
     2461 cacaaattcc tgctctcggc tgcccgcata caccaaatga gtatgcgaat cgcaaaagct
     2521 agggaaaacc atccctttat ctgcatctat cacctcaatg tccggtaagt tgacaaattg
     2581 ttgaaattca gacgaatcca tttctccata ccccctgatc tcatctcctt cgataagtaa
     2641 ccaagcgttt gaaatcgtgt tcaacttctg catgtccttg ccaactctaa ctgctctgcc
     2701 actttcatct acattcacca gttgctgaat attggtaatg attatcatgt ttttagggtt
     2761 aatttgaatg aattgattgt tctgatttaa gtcgatttca gtatagattt atttcaaata
     2821 tattgaacaa gcatgttctt gcaatagcct gccttccccg atgattcaga attccgttgc
     2881 aagcaagcca ctgcaaatct tttactcgaa gcaccataca aaaagacatt tgagctcaaa
     2941 ttttctaagc atcaaaggta agatattcga gcgaaccaaa tccaaactcc gacctcttac
     3001 tccaattctt ttccataaat ctctatggcg ttcaatgcaa acctcaatca ttcaaattgg
     3061 ctctaatcaa tctggtttaa gcttaaaaat ctgtccatac atcttcaaag attctgagtg
     3121 atattaagtt ttattctaaa ctaatgcgaa tttttaaaat ttaatcgatt aaccttattt
     3181 atccctacgg gacttatccc aacttgagca ttttaccctg aaaaaacagg aaaaaataga
     3241 gttttt
//
LOCUS       DAORPD010000075         3244 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_5236_length_3244_cov_2.233302, whole genome shotgun sequence.
ACCESSION   DAORPD010000075 DAORPD010000000
VERSION     DAORPD010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5236_length_3244_cov_2.233302"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1454)
                     /locus_tag="PK976_01425"
     CDS             complement(<1..1454)
                     /locus_tag="PK976_01425"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRC66580.1"
                     /translation="MNRRILAIIIFLMGFVLVVLLFIQVYWIRNALMLREAAFTAGVE
                     EAVSNVVLSLERIESGKRLRSFKESARLYRKIDSLNYLIDKRYHELTGEDGSSAADVK
                     ARRKIIQDRKSWRTLRDLDTTSEFRENRNIQEELDSLIEPKQIQTIQTRSVNIEDDPV
                     YKRLQKQRESVVEELMSRSLIYDAALGRFDDIIEMPLEKRIQPGIVDSMLAVELQNKG
                     IKTEFEFGIYDTRRDSLIYQKTGKFTQELLNKAFAVNLFPRQPGKDKAILLVYFPYER
                     QYMVQQIWWLLLLSILLVGTFIAIFYKTISTILYQKKLSDMKNDFISNITHEFKTPVS
                     TISLACEALNDPDVVKTPAMASNYIQIITDENRRLGTMAEKILIASVLEQGKLELNMV
                     PCAMHEIITECVQKVGIQAETAGGSIQTSLQALHDMVRGDRLHLTSVVVNLLDNAIKY
                     SPIRPTIRINTYNRDQNFVFEVIDNGIGISKENQG"
     gene            1639..2199
                     /locus_tag="PK976_01430"
     CDS             1639..2199
                     /locus_tag="PK976_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66581.1"
                     /translation="MKRVVCFLGMALCGVILLGGLQSCDKVENLAKFDVKMDLPSLNF
                     NLVASAKNSSTQEYYKDFNVYLNLDSIQEAHSLNSFSIENGKLTKATLRIIYPAGANF
                     QFLINSRLVLFNSSNQEMQVAHTGNINANATEIELILDTADLTSLFKMKHYTGRIYYT
                     IEPSLLPAQNVTIQLNQSIQFTVHPL"
     gene            2313..>3244
                     /locus_tag="PK976_01435"
     CDS             2313..>3244
                     /locus_tag="PK976_01435"
                     /inference="COORDINATES: protein motif:HMM:NF012562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66582.1"
                     /translation="MLPTIDPTTTKSWKKLSNYFTSIGKQSLNQLFESDNNRAQKFSL
                     QWQNLFVDYSKTHIDEQTMRLLIELARECHLAEAIEQMFRGEKINQTQKRAVLHFALR
                     NLGDDPVFLDGRDVMPEIRTVRNQMKIFSTQVRNGDWKGYTGKPIRNIVNIGIGGSDL
                     GPAMVCRGLQFYGHERLRVFFVSNVDGGHIYETLRHLNAEETLFLIVSKSFTTQETLA
                     NAKTARQWFINQGGKAEDISKHFVAISTNTPAAMEFGIDPENIFHFWDYVGGRFSLWS
                     AVGLSIALQIGYESYEELLKGAEAMDHHFRETPF"
BASE COUNT          978 a          633 c          638 g          995 t
ORIGIN      
        1 ccttgatttt ctttactaat tccgatgcca ttatcaatca cttcgaatac aaaattctga
       61 tcgcggttat aagtatttat cctgatggta ggccttatgg gagaatattt aatggcatta
      121 tcgagtaagt ttactacgac gcttgtaagg tgcaggcggt cgccccgcac catgtcgtgt
      181 agggcttgga gggatgtttg tatgctgcct ccggcggttt ctgcctgtat accaaccttt
      241 tggacacatt ctgtaattat ttcatgcatc gcacaaggta ccatgttcaa ttcgagtttt
      301 ccctgctcga gtaccgatgc tatgagaatt ttttcagcca ttgtacctaa acgtctgttt
      361 tcgtcggtaa ttatctgaat atagttcgaa gccatagcgg gtgtttttac cacatcggga
      421 tcgttcaagg cttcacacgc aagcgaaatc gttgaaaccg gagtcttgaa ttcgtgtgtg
      481 atattgctaa taaaatcgtt cttcatatcc gataatttct tttgatacag aatagttgaa
      541 atggttttat agaatatggc aataaatgta ccaaccaaaa gaatggaaag taaaagcaac
      601 caccatatct gttgaaccat atactgtcgt tcgtagggaa aataaaccag caggatggct
      661 ttatctttgc caggttgacg gggaaacaga ttaacagcaa aggctttatt cagaagctcc
      721 tgagtaaact tgccagtttt ttgataaatg agactatcgc gtctcgtatc gtatatacca
      781 aattcaaatt ccgtcttgat gcctttattc tgaagctcta ctgccagcat agaatctact
      841 ataccgggtt gtattctttt ttcgagtggc atttcgatga tatcgtcgaa acgaccaaga
      901 gctgcatcat agataagaga acggctcatc agctcctcca ctacgctttc tctttgtttt
      961 tgcagtcttt tgtaaacagg atcatcttcg atatttacag atcgagtttg tatagtttga
     1021 atttgttttg gctcgattaa agaatcgagt tcttcctgaa tattcctatt ttcacgaaat
     1081 tctgaagttg tgtctaaatc acgcagagta cgccatgatt tacgatcctg aattattttt
     1141 cgacgtgctt ttacatcggc tgccgaactg ccatcttccc cagttagctc atggtaacgc
     1201 ttatcgatta aataattcaa cgaatcgatc tttcggtaaa gtcgggcact ttccttgaag
     1261 ctgcgcaaac gcttgccaga ttcgatcctc tctaaagaaa gcacaacatt cgatacggct
     1321 tcttcaaccc ctgccgtaaa tgcagcttca cgcagcatga gagcattcct gatccagtaa
     1381 acctgaataa acaataggac caccaataca aagcccatta aaaagatgat aatagcgaga
     1441 atacgtctgt tcattttcgt tacaaaatta actgaaaatc aaatgaccat cgaaattcta
     1501 tgcctttttc gcaatcttct aaaaaaatcg aaaaatttta aaaaatttag tctgtttgtt
     1561 ataaaagatg ataaaaagag tgagatatcc tgtaattttg taagattaaa atcctgcaat
     1621 caaataatcc aaaatcccat gaaaagagta gtgtgttttt taggaatggc cttgtgtggt
     1681 gtcattttat taggtggact gcaatcgtgc gataaagtgg aaaatcttgc caaattcgat
     1741 gttaagatgg atttaccatc ccttaatttt aatttagtgg catctgctaa aaactcctca
     1801 actcaggaat actacaaaga tttcaatgtt tatttaaatc tcgatagtat tcaggaagct
     1861 cattcattga attcgttttc catcgaaaat gggaaactta cgaaagcaac cttgcgcatc
     1921 atttatccag caggagcaaa ttttcagttt ttgatcaact ctcgtttggt attgttcaac
     1981 agcagcaatc aggagatgca agtggctcat actggcaata tcaatgccaa tgccactgaa
     2041 attgaattga ttctggatac tgccgatctt acttcgttat tcaaaatgaa gcattatact
     2101 ggcagaatct attacaccat agagccctcg ctgttgcctg ctcaaaatgt taccatacag
     2161 ttgaatcaaa gtatccagtt tactgtacac cccctgtaga atcccacatc tttgtccgaa
     2221 ggcattgaaa atcccggtgt gaatagcgct gggatttttt atttttgcat catgataaat
     2281 gttattctaa ctcatcaata tattttttcg atatgttacc aacgatagat cctacaacaa
     2341 ccaaaagctg gaaaaaatta agtaattatt tcacgagcat cggcaagcaa agtctgaatc
     2401 aattattcga aagtgataac aacagagcac aaaaattttc tcttcaatgg cagaacttgt
     2461 tcgttgatta ttcgaaaacg catatcgacg aacaaactat gcgattgctt atagaacttg
     2521 ccagggagtg ccatctcgcc gaagccatag agcaaatgtt tagaggggaa aaaatcaatc
     2581 aaacgcaaaa aagagctgtt ttacattttg ctttacgcaa ccttggcgat gatccagttt
     2641 ttcttgacgg cagggatgta atgccagaaa tacgaaccgt acgtaatcag atgaaaattt
     2701 ttagcactca ggtaagaaat ggtgattgga aaggatatac cggtaagccc atacggaata
     2761 tcgtcaatat tggaattggt ggtagcgatc ttggcccggc tatggtttgc agagggttgc
     2821 agttttatgg tcacgaaaga ttaagggtat ttttcgtatc gaacgttgac ggaggtcata
     2881 tttatgaaac tcttcgccat ttgaatgccg aagagacttt atttcttatc gtttcgaagt
     2941 cgtttacgac tcaggagaca ctggctaatg caaaaactgc ccgacaatgg ttcattaatc
     3001 agggcggtaa agcggaagac atcagcaaac attttgtagc tatctcaaca aataccccgg
     3061 cggcaatgga atttgggatc gatcctgaaa acatatttca tttctgggat tatgtaggcg
     3121 gtcgattttc attgtggtca gccgtagggt tgagcattgc attgcaaatt ggttatgaaa
     3181 gttacgaaga actattaaaa ggtgctgaag ctatggatca tcacttcaga gaaactccct
     3241 tcga
//
LOCUS       DAORPD010000076         3238 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_5250_length_3238_cov_2.329877, whole genome shotgun sequence.
ACCESSION   DAORPD010000076 DAORPD010000000
VERSION     DAORPD010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5250_length_3238_cov_2.329877"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2075)
                     /locus_tag="PK976_01440"
     CDS             complement(<1..2075)
                     /locus_tag="PK976_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRC66583.1"
                     /translation="MKVYQTKEIRNIAIVGAAKTGKTTLAEDMLYEGGVINRRGSIED
                     KNTVSDYRDIELERQNSIYSTVLYAELNDKKINMIDCPGFDDFVGEVLAALKVTDTAL
                     MLINAQNGVEVGAEINFRNASKAKMPLIFVINHLEHENSNFDETIRQMRQSFGGGVMI
                     CQYPVNEGLGFDAVIDLLQMKMLKFNASGNTEILEIPASEKVKADEMYSAMIEELAAN
                     EESLMEKFFENGSLTEEEISEGLKIGLINRGIFPVFCIHAKQNLGVRRLMEFIISSVP
                     SPDEAPRAKTIDGNEVTYNPADPACAFIFKTTMEPHLGEMSFFKVYTGEITEAMDMIN
                     AENGSKERMSQLFAVAGRNRQKIEKVMPGDIAATIKLKNVRTNHTLNSPKAPDVKIEP
                     IVFPAPKISFAVKAKNSTDDEKVILILNDLAKIDPTLVVEQSKELRQLIVSGQGELHL
                     NIQKWLIENIYKIPIDFLPPRIPYRETITKPAKSTYRHKKQSGGAGQFGEVHMMIEPY
                     YDGMSNQSEFPIRTQEVYDLDWGGKLVFNNCIVGGAIDARFMPAILKGIMEKMEQGPL
                     TGSYARDIVVNIYDGKMHPVDSNEISFKLAGRNAFKEAFKNAGPKILEPIYEVEVILP
                     EDKFGDVMTDLQGRRAIILGMDSEGNYKKIIARVPLAEMNRYSTALSSLTSGRATYSL
                     KFVEYAQVPP"
     gene            complement(2174..3166)
                     /locus_tag="PK976_01445"
     CDS             complement(2174..3166)
                     /locus_tag="PK976_01445"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HRC66584.1"
                     /translation="MKHILGIGNALVDMITSIPGDDVLHHLSLPKGSMTLVDAALAQN
                     ITKTTSKFSPKLTSGGSAANTIFGLAHLGLQVGYLGKTGKDELGEIYENDLKACGITT
                     HISKSTTPTGRAVALISPDSERTFAVYLGAALELSPYDIMESILAQYAHVHIEGYQVQ
                     NHEVLLKILERAQACGCSISLDLASYNVVEENREFLQYILNNYVNIVFANEEEARALT
                     GEEPIGALDKLSQICPMAIVKIGKNGSMIKTNEKVWKIDAIEANAIDSTGAGDMYASG
                     FLYGLVHHYDIGTSGKIASLVAGKVTEIEGARLPGLVWDEIRQQIQWFIKNSVR"
BASE COUNT          872 a          739 c          618 g         1009 t
ORIGIN      
        1 ggcggaactt gggcatattc gacaaatttc aaggaatatg tagctctacc tgaggtaagt
       61 gaactgaggg cagtggagta acgattcatt tctgccaatg gaacacgggc aataatcttt
      121 ttatagtttc cttcgctgtc cattccaagg ataatagccc ttcgtccctg caggtcggtc
      181 attacatcac caaatttatc ttctggaaga atgacttcta cttcatagat aggctccaag
      241 attttgggtc cggcattctt gaatgcttcc ttgaaagcgt tacggcctgc caatttgaag
      301 gaaatttcat tggaatcgac cgggtgcatc ttaccatcgt aaatattgac gacaatgtcg
      361 cgagcgtatg atcctgtgag aggaccttgt tccatttttt ccatgattcc cttgagaatt
      421 gccggcatga accttgcatc gatagcacct cccacaatgc agttgttgaa taccaatttc
      481 ccaccccaat ccagatcgta cacttcttgt gtgcgtatcg gaaactctga ctgattggac
      541 atgccgtcat agtagggttc tatcatcatg tgtacttcac caaactgacc tgcacctccc
      601 gattgtttct tatgacgata tgtggatttg gcgggcttgg ttatggtttc tcgataaggt
      661 attcgtggcg gcaagaaatc tattggaatc ttataaatgt tttcgatgag ccacttttga
      721 atgttaagat ggagttctcc ctgtccactc acgataagct gtctgagttc tttcgattgc
      781 tcgacaacga gcgtgggatc gatttttgcc agatcattga gaatcagtat cactttttcg
      841 tcatcagtgc tatttttagc ctttaccgca aacgagattt tgggtgccgg aaaaacaatg
      901 ggttcaattt tgacatcggg agctttgggc gaattcaagg tatggttggt acgtacattc
      961 ttcagcttta tggtagcggc aatatcccct ggcatcactt tttcgatttt ctgacgattt
     1021 cggccagcca cggcaaaaag ctgagacatc cgctccttcg aaccattctc ggcatttatc
     1081 atatccatgg cttcggtgat ctcaccggta tataccttga agaagctcat ttcgcccaga
     1141 tggggttcca tggtggtttt gaatataaaa gcacaagctg gatcggctgg attgtaagta
     1201 acttcattcc catcaatagt tttggctcgt ggtgcttcat ccggagaagg cacagaactt
     1261 ataataaact ccatcaaacg acgaaccccc aaattttgct tggcatgaat acaaaaaacc
     1321 gggaagattc cacgattgat caaacctatc ttcaaacctt cactgatttc ttcttcagtt
     1381 aaagagccat tttcgaaaaa cttctccatc agtgattctt cgtttgcggc aagttcttct
     1441 atcatggccg agtacatttc atcggctttc accttttcag aagcgggtat ttctaaaatt
     1501 tccgtatttc ctgaagcatt gaatttcagc atcttcatct gcaaaaggtc gatcacggcg
     1561 tcgaaaccaa gtccttcgtt tacaggatat tgacaaatca ttactccacc tccaaagctt
     1621 tgccgcattt gacgaatggt ttcatcgaag ttagaatttt catgttccag atgattaatc
     1681 acaaaaatca aaggcatctt ggctttggaa gcattgcgga aattgatctc ggctccaact
     1741 tcaacgccat tttgagcatt gatgagcatg agcgccgtat cggtaacttt gagggcagcc
     1801 aatacctctc ctacaaaatc atcaaaaccc gggcaatcga tcatattgat tttcttgtca
     1861 ttcagctcgg catataaaac ggtggaatat atcgagtttt gacgctccag ctctatgtcg
     1921 cgataatcgg aaaccgtatt cttatcttcg atagaacccc tacgattgat gactccacct
     1981 tcatatagca tatcttctgc aagtgtggtt tttcccgttt tcgccgcacc tacaatggca
     2041 atatttctga tttctttcgt ttgataaacc ttcatttgtt taaatggtta taatttaagt
     2101 atttgcaatt gagcggcaaa attataaaaa agccttcgat tgccaaatgt ttgcgcccac
     2161 taaaaatttt tcgctatcta accgaatttt taataaacca ttggatttgc tgtctaatct
     2221 catcccagac taaaccgggc aacctggcac cttcgatttc ggttactttc cctgcgacca
     2281 acgaagcaat ctttccgctg gttcctatat cataatgatg cacaagccca tagagaaatc
     2341 cagaggcata catatctcca gctcccgtac tgtcaatggc attggcttct attgcatcga
     2401 ttttccaaac tttttcattg gttttaatca tcgaaccatt cttcccaatt tttacaatgg
     2461 ccattgggca tatctgcgaa agtttgtcaa gtgctccaat aggttcttct cccgttaagg
     2521 ctcttgcctc ttcctcattg gcaaaaacaa tattaacgta attattcaaa atatactgca
     2581 aaaattctcg attttcttct actacattgt aactggccaa atccaagcta atactgcaac
     2641 cgcaagcctg cgccctttcc aatattttta acaatacttc gtgattctgt acctgataac
     2701 cttcaatatg aacatgagca tattgtgcca gaatgctttc cataatatcg taaggcgaca
     2761 actcaagagc agcccccaaa taaactgcaa aagtacgttc agaatcggga ctgataagcg
     2821 ctactgccct gcccgtgggt gtcgtacttt ttgaaatatg cgtcgtgatt ccgcaggctt
     2881 tcagatcgtt ttcgtaaatt tcgcctaact catcttttcc tgttttacca agataaccca
     2941 cttgtagtcc aagatgagcc aaaccaaaaa tggtatttgc cgctgaacct ccactggtta
     3001 acttgggtga gaatttagaa gtggttttag taatattttg ggctaatgca gcatccacca
     3061 aggtcatact acctttgggc aaggacaaat gatgaagaac atcatcgccc ggtatactgg
     3121 taatcatatc tactaaagca ttaccaatgc caagtatgtg cttcatgagt ctgtggtctt
     3181 tacaaataaa atttttgcaa atgtagccaa agtaagaaat ttttattaat tttgctcc
//
LOCUS       DAORPD010000077         3219 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_5283_length_3219_cov_2.391593, whole genome shotgun sequence.
ACCESSION   DAORPD010000077 DAORPD010000000
VERSION     DAORPD010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5283_length_3219_cov_2.391593"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="PK976_01450"
     CDS             <1..533
                     /locus_tag="PK976_01450"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRC66585.1"
                     /translation="IISRIVYHQLEDIIAEVFEKGRSPFIVIIDRVTDVRNLGAIART
                     AECAGVDALVVPLHESAMIHAEAIRTSSGALARIPICRVDNLKWAVNYMKASGLSIIS
                     ISEKGNCSFSEINFTIPVALILGSEEDGISNALLRLSDEICFVPMMGKIGSLNVSVAG
                     GIAMYEVVRQRINKNQ"
     gene            534..2471
                     /locus_tag="PK976_01455"
     CDS             534..2471
                     /locus_tag="PK976_01455"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRC66586.1"
                     /translation="MMERIKNWITRHPYDALFILVILAGAVLRLYDFSCWSLSNDELS
                     ALNRLRSDSFSELIQNGVRPDGHPAFTQVFLYYWTAIFGLQPFWVRLPFVLAGIASIW
                     LVYAIGKQWFNAATGLYASLFIAVLPFTILYSQLARPYSFGLLFTLLTAYFWGRYFLL
                     HHYGWGTLAGFALSAAFAMYTHYFSFMMAGLFILAGFLFISRDNWKGFLISIATIFLL
                     YLPYLNIFLFQLSLGGVGGAQGWLTKPKWDWIFNFFYYAFGLSAFHLLLLLAFLTIFM
                     VWYVKNTHIRKIQWVLLAIFLLPYLIGFVYSILVNPVLQHSGLLFAFPFLLLFLFSFF
                     DDDRPVVSSIFVLVGLIGGSWVALDKTDDFRNSQFADFKACTSVYMEWENKYRKDSIT
                     LTASINHPYYLEYYFHNAHKNPPQIIVLESKQGRELADLVQILEQSRTPYFAYFRLKP
                     APIEIPDVIAGTYPVLLDYRDYFGQAEAFLFAKDTSLKGIPKPKPISNFYCDFEGDDE
                     LFGVRISNLDTICCSGKYAMHLRPEMEWGPGIKITTRQAELRSPSRVRIKVEVLTDST
                     LIDSPIVLTLTDQFDKQYIWLSGKLENFLTPGKWGTAYFTADLPHPYSLGDELKIFIW
                     NKDHRNLWVDNFSIEFYKLEK"
     gene            2491..>3219
                     /locus_tag="PK976_01460"
     CDS             2491..>3219
                     /locus_tag="PK976_01460"
                     /inference="COORDINATES: protein motif:HMM:NF016103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HRC66587.1"
                     /translation="MKKLFFIFCGFLVLTSFSTDKPAWKLFDKSGKECNYQNLLDEAS
                     KADVVLFGELHDNPISHWLQYELLSDLYRMKGAQLVTGAEMFETDNQLLLDELIAGQI
                     SLKNFEAEAKLWPNYSTDYKPMVEFALEHRIPFIGTNIPRRYAAMVNRKGFEALNGLS
                     NQAKMLIAPLPVQYDSALPAYKAMLNMGGHLGANLPKAQAIKDATMAWSILKYLQAGE
                     IFLHLNGAYHSDNYEGIVWYLRKYR"
BASE COUNT          859 a          648 c          668 g         1044 t
ORIGIN      
        1 ggataatttc tcgaatcgtt tatcatcagc tcgaagatat aattgctgag gtctttgaaa
       61 agggccgttc tccttttatt gtaatcatcg acagggttac cgatgtgagg aatcttggtg
      121 ccatagccag aactgccgaa tgtgctggtg tagatgctct tgtggttccc ttgcatgaat
      181 cggccatgat acatgctgaa gccatcagga cttcttcggg tgcattggca cgcattccca
      241 tttgtagggt agataatctg aaatgggcgg taaattatat gaaagcaagt ggtttatcca
      301 ttatttccat ctcagaaaaa gggaattgtt ccttctcgga aataaatttt accataccgg
      361 tagcccttat tttaggcagt gaagaagacg gtatcagcaa tgctttgctt cgcttgtcgg
      421 atgaaatttg ttttgttccg atgatgggaa agattggctc gttgaatgtt tccgtagcag
      481 gaggtattgc aatgtatgaa gttgtgcgtc aacgaataaa taaaaaccaa tagatgatgg
      541 aaaggataaa aaattggatt acacggcatc cttacgatgc attgtttatt ctggtcattt
      601 tggcaggagc ggtattgagg ctatatgact ttagctgctg gtcgttgagt aatgatgaat
      661 taagtgcctt gaatcgcctg cgttccgaca gtttctcaga gcttatccag aatggtgtcc
      721 gtcctgatgg tcacccggcc tttacacagg tttttctgta ttactggacg gcaatttttg
      781 gtttgcaacc tttctgggta cgactaccct tcgttttggc aggaatagct tctatttggc
      841 tggtttatgc cattggcaaa caatggttca atgcagctac cggcctatat gcatctctat
      901 ttatagccgt tttgcctttc acgattttat acagccagct tgcacggccc tatagcttcg
      961 gcttattatt taccttgctt acagcttatt tctggggaag atattttctt cttcaccact
     1021 atggctgggg cactctggcc ggttttgcac tttcggctgc attcgccatg tacacccatt
     1081 attttagctt tatgatggcc ggcctcttca ttttagccgg atttttattc atctcccgag
     1141 acaactggaa aggttttctc atttcgatag caactatctt tttactttat cttccatatc
     1201 tgaacatctt cttgtttcaa ttatcgcttg gcggagtggg aggagctcag ggatggctaa
     1261 ccaaacctaa atgggactgg atttttaatt tcttctacta tgcattcgga ctttctgctt
     1321 ttcatctgtt attgttactg gcttttctga cgatttttat ggtatggtat gtaaaaaata
     1381 cacacatccg aaaaatccaa tgggttttac tggctatatt cttattgccg tatctgatag
     1441 gttttgttta tagcattctt gtcaatccgg ttttgcagca cagcggtctt ttgtttgctt
     1501 tcccatttct tcttttgttt ttattctcat ttttcgatga cgatcgccct gtggtttctt
     1561 ctatcttcgt tttagtaggt ttgataggag gttcatgggt agcactggat aaaaccgatg
     1621 atttccgaaa ttcacaattt gccgatttca aggcctgcac ttcggtttac atggaatggg
     1681 aaaataaata ccggaaagac agcattaccc ttaccgcatc catcaatcat ccctattatc
     1741 tcgaatatta ttttcacaat gcgcataaaa accctcctca aattattgtt ctggaatcga
     1801 aacaaggccg tgaactggct gatctcgtac aaatattaga acagagccga acaccctatt
     1861 ttgcttattt tcgtttaaaa cctgcaccca tcgaaattcc agatgttatt gcaggtacat
     1921 atcctgtgct gctcgattac agagattatt tcggtcaagc cgaagcattt ctttttgcaa
     1981 aagatacttc cttgaaaggc atccccaaac ccaagcctat aagtaatttt tactgtgatt
     2041 ttgaaggcga cgatgagctt ttcggtgtta gaatatccaa tctcgatact atctgttgca
     2101 gtggaaaata tgccatgcat ctgcgtcctg aaatggaatg ggggccaggt ataaaaatta
     2161 cgacccgaca ggctgaactt cgctcacctt caagagtaag aataaaagtg gaagtcctaa
     2221 cagatagtac gctcatcgat tcgcccattg ttcttacact taccgatcag tttgacaaac
     2281 aatacatttg gcttagtggt aaactcgaaa acttcttaac cccaggtaaa tggggcacag
     2341 cctattttac tgccgatttg cctcatcctt attccttggg cgatgaactg aaaattttta
     2401 tctggaataa agaccataga aatctatggg ttgataattt ttcgatcgaa ttttataaac
     2461 ttgaaaaatg aattaaaaaa caaactaaac atgaagaaat tatttttcat tttttgtgga
     2521 tttttggttc tcacttcttt ttcgacggat aaacctgcat ggaaattatt cgataaatcg
     2581 ggcaaggaat gtaattatca aaatttgttg gatgaggcat cgaaagccga cgtggtattg
     2641 tttggcgagt tgcatgataa tcccatcagc cattggcttc aatatgagct gttatctgat
     2701 ttgtacagaa tgaaaggtgc tcaacttgtt actggtgccg aaatgttcga aaccgacaat
     2761 cagcttctgc tcgatgaatt gatagcaggg caaatcagct tgaagaattt cgaggccgaa
     2821 gccaaattgt ggccaaatta ttcgaccgat tacaaaccca tggtggaatt tgcactcgaa
     2881 catcgaatac cattcattgg gacgaatatt ccccgcaggt atgctgctat ggtaaatcga
     2941 aaaggattcg aagcactcaa tggtttaagt aatcaggcca aaatgttgat agctccctta
     3001 cctgttcaat acgattctgc cttgcctgcc tataaagcta tgctaaatat gggaggacat
     3061 ctgggagcaa atcttcccaa agctcaagcc atcaaagatg ctaccatggc atggtccatt
     3121 ctgaaatatc tgcaagccgg tgaaatcttc ttgcatttaa atggtgctta ccactccgac
     3181 aattatgaag gaatagtgtg gtatcttcga aagtatcgc
//
LOCUS       DAORPD010000078         3213 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_5297_length_3213_cov_2.150412, whole genome shotgun sequence.
ACCESSION   DAORPD010000078 DAORPD010000000
VERSION     DAORPD010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5297_length_3213_cov_2.150412"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /gene="hflX"
                     /locus_tag="PK976_01465"
     CDS             complement(<1..696)
                     /gene="hflX"
                     /locus_tag="PK976_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HRC66588.1"
                     /translation="MIERVNKKETAVLIGLITPEQPVEKLNEYLDELEFLADTAGADV
                     RKRFVQRLPYPDPRTFVGKGKLKEVFDYVVENAIDLIIFDDELTASQLRNLDRELPCK
                     ILDRNNLILDIFAHRARTSHAIAQVELAQYEYMLPRLARMWTHLEKQRGGIGLRGPGE
                     KEIETDRRLIRKRIALLKNQLEQIDQIKQTQRKSRTNLVRVALVGYTNVGKSTLMNVL
                     SKSSVFAEDKLFAT"
     gene            complement(887..1624)
                     /locus_tag="PK976_01470"
     CDS             complement(887..1624)
                     /locus_tag="PK976_01470"
                     /inference="COORDINATES: protein motif:HMM:NF003198.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HRC66589.1"
                     /translation="MEVYSKISVNSSEGKGMFAILIDPDKIHHNSFRTVAHKAAEADV
                     DFLFIGGSLLSADIMEECLYEVRCETDIPLVIFPGSVFQINKAADAILLLSLISGRNP
                     EMLIGNHVVAAPYLKASQLEIIPTGYMLIESGVSTTALYMSCTFPIPHSKDEIAVNTA
                     MAGEMLGLKLIYLDAGSGATSPVSASMITKVKQAIGIPLIVGGGIDTPEKARRAREAG
                     ANLVVVGNAIEKDSRLIHEIAYAIHEI"
     gene            2104..3096
                     /gene="dusB"
                     /locus_tag="PK976_01475"
     CDS             2104..3096
                     /gene="dusB"
                     /locus_tag="PK976_01475"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HRC66590.1"
                     /translation="MTVSFMKIGSLEISYPFIALAPMEDITDTSFRRICKKLGADMVY
                     TEFISSQGLTREGEKSLEKLKFSEEERPIGIQIFGNETTSMVEAAKIATTVQPDLIDI
                     NWGCPVKKVAGKGSGSGILNDIPKMISITQAVVKATHLPVTAKTRVGYDESNKPIVEI
                     TERLQDAGVAGITIHGRTRSQLFSGEADWTLIGKVKENPRIKIPIIGNGDIVSAHFAK
                     TMLERYHVDGIMIGRAAIGNPWIFDEVKTFLNSEILLPPPNINERINVLIEHLQIAIQ
                     IKGERRAILEMRKHYSGYFKGMPNFKKIRTQLMTFNYFHEIDDFLQNVMQAGLT"
BASE COUNT          996 a          690 c          587 g          940 t
ORIGIN      
        1 ggttgcaaaa agtttatctt cggcaaatac actcgatttg ctcaatacat tcatcagggt
       61 tgattttcct acattcgtat aacccacaag agccaccctc accagattag tgcgtgattt
      121 gcgctgagtt tgctttatct ggtcaatttg ttccaattga ttctttaaca aggcaatacg
      181 ttttcgaatc aaacggcggt cagtttcgat ttccttttct ccaggtcctc tcaatccaat
      241 gccgccacgc tgcttttcga ggtgagtcca catacgtgca agacgaggca gcatgtattc
      301 atattgtgca agctccacct gagcaatggc atgggaggtg cgtgccctat gtgcaaagat
      361 gtcaaggatc agattgttac gatctaatat tttgcacgga agttccctgt ctaaatttcg
      421 taattgtgag gcagtaagtt catcatcaaa aataatcaga tctatggcgt tctcaactac
      481 ataatcgaaa acctccttca atttaccttt cccgacaaaa gttctcggat cggggtaagg
      541 aagacgttgt acgaatcttt ttctaacatc ggctccagcg gtatcggcta aaaattcgag
      601 ctcatccaga tattcattca atttttccac cggctgttcc ggcgtaatca atcctattaa
      661 aactgcagtc tcttttttgt taaccctttc aatcatacat catccattcc cacttgatgc
      721 acgaagcaat tttaattcca attcgaaatt tttcgaaaaa atcatggaag gctggattga
      781 atgggaaaaa atcattctat aaattgtgtc agcgttgatt attctcagtc gggaatgatt
      841 aaaagatcgg gagctctatc gttgttcata caatttcagg cacatgctaa atttcgtgta
      901 tcgcataagc tatttcatgg attaacctcg aatctttttc gatggcatta cccactacta
      961 cgaggttggc tccggcttct ctggcccgac gagctttttc gggtgtatcg attccccctc
     1021 ccacaatcaa agggatacca atagcttgtt ttactttggt tatcatagaa gctgaaactg
     1081 gcgaagtagc accacttcct gcatccaaat aaatcaattt caatccaagc atttcaccgg
     1141 ccatagcagt atttactgca atctcatctt tagaatgggg gatggggaag gtgcagctca
     1201 tatacaaggc tgtagtcgaa acgccacttt caattagcat ataacccgta ggaatgattt
     1261 ctaattgact ggccttgaga taaggtgcgg caactacgtg attcccaata agcatctctg
     1321 gattcctccc cgagattaag cttaagagca aaatggcatc ggctgccttg tttatttgga
     1381 aaacactgcc cgggaaaata accaaaggta tatcggtttc gcatcgaact tcatataaac
     1441 attcttccat aatatcggca ctaagcaaac tgcctccaat aaataaaaaa tctacatctg
     1501 cttcagcagc tttatgagct actgttctaa aactattatg atgaatctta tcgggatcta
     1561 tcaaaatggc aaacatcccc ttaccttcgc tactattgac cgatattttc gaataaacct
     1621 ccatggttct atgctctaag gtgcaaatat aaaaattatt tttttgaaag taatttgtaa
     1681 attttagaag ttggaattca aattttgatg attaatagtt gaagagtcaa aaaaaactat
     1741 gtaaatctgg tgaaaatcaa tcaaatggct agtaattaac gacatttcga aatatttatt
     1801 aggcaagaat tcaaacttcc tatgataagt tttcaatgat ttacactccc ataaatttat
     1861 attcaaaacg gtcacactcc taatgaagtg gtcaaaagaa cgtgaaacgg aggtcggaaa
     1921 atttcccaat ctcaaaatgt tccagtctta aaatctcatc atctgctttc acctcgccat
     1981 ccggaccacg gaatacttct cacgcaatgc ttttcacatt tttctccaac ctacaaaatg
     2041 ccttctacct cttaattcat gctgcttttt tctttttact atcttagcaa ttaaaatgct
     2101 tatgtgactg tttcatttat gaagatagga tcattggaaa tcagctatcc atttattgca
     2161 cttgctccta tggaagatat cacggataca tcgtttcgaa ggatatgcaa gaagttgggt
     2221 gccgatatgg tttacacaga atttatctca tcgcaaggtt taacaaggga aggagaaaaa
     2281 agtttagaaa aactgaaatt ttcagaagaa gaaaggccta ttggaataca aatctttggg
     2341 aatgaaacaa caagcatggt agaggcagca aaaattgcaa ccacggttca acccgatttg
     2401 atcgatatta actggggatg ccccgtaaag aaagtcgcag gcaagggttc tggctctggt
     2461 attcttaacg acatccccaa gatgatctca atcacccagg ccgtagtgaa ggccactcat
     2521 ttgccggtta cggccaaaac cagagtgggc tacgacgaat cgaacaaacc cattgtcgag
     2581 atcaccgaaa gactgcagga tgccggagtt gccggtatta ccattcatgg cagaactcgt
     2641 tcacagttat ttagtggaga agccgactgg actcttatcg gaaaagtaaa ggaaaatcca
     2701 cgcataaaaa ttcccattat tggcaatggc gatatcgtat cggcccattt cgctaaaacc
     2761 atgctcgaac gctaccatgt agatggtata atgattggac gggctgctat tggcaatccc
     2821 tggatattcg acgaagtaaa aaccttcctc aattctgaaa ttttgcttcc acctcctaat
     2881 attaatgaaa gaatcaacgt tttgatagaa catttgcaaa tagccatcca aataaaaggc
     2941 gaacgcagag ctatcctcga aatgagaaaa cactacagcg gctatttcaa gggaatgccc
     3001 aacttcaaga aaatccgaac acaactgatg acattcaatt atttccatga aattgatgat
     3061 ttcttacaga atgttatgca ggccggcctc acctgaaatg acttctataa aagaaaatta
     3121 gaacacagtc aatggtatat tcaaaattta ttaatagccc tacctcctat ctatatccct
     3181 tcatttcctt ctaattatgt aaaactaaca gca
//
LOCUS       DAORPD010000079         3197 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_5335_length_3197_cov_2.508912, whole genome shotgun sequence.
ACCESSION   DAORPD010000079 DAORPD010000000
VERSION     DAORPD010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5335_length_3197_cov_2.508912"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1297)
                     /locus_tag="PK976_01480"
     CDS             complement(<1..1297)
                     /locus_tag="PK976_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRC66591.1"
                     /translation="MAFRKDPTAPISNFNQIMISLASPESILERSYGEVLKPETINYR
                     TYKPERDGLFCERIFGPVKDWECHCGKYKRIRYKGIVCDRCGVEVTEKKVRRERMGHI
                     QLVVPVAHIWYFRSLPNKIGALLGLPTKKLESIIYYENYVVINPGIMSSEGISYMDFL
                     SEEDYFKIIENLPPENQNLDDSHPDKFIAKMGGEALYDLLSRIDLDKESFDLRYKASI
                     ETSQQRKQDILKRLQVFEAFREANRRTENRPEWMMVKVVPVIPPELRPLVPLDGGRFA
                     TSDLNDLYRRVIIRNNRLKRLIEIKAPDVILRNEKRMLQEAVDSLFDNSRKASSVRTE
                     SNRALKSLSDSLKGKQGRFRQNLLGKRVDYSGRSVIVVGPELKLNECGLPKEMASELF
                     KPFIIRKLLERGIVKTVKSAKKIVDRKDPVVWDILENVLK"
     gene            complement(1364..>3197)
                     /gene="rpoB"
                     /locus_tag="PK976_01485"
     CDS             complement(1364..>3197)
                     /gene="rpoB"
                     /locus_tag="PK976_01485"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008152451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HRC66592.1"
                     /translation="VPIIGTGIEEFVARDSRVLINAEGNGVVEYVDATRVVIRYERND
                     MERLISFDEDVKTYYLIKFKRTNQNTTINLRPIVRKGDKVVKGQVLCEGYATSNGELA
                     LGRNLMVAFMPWKGYNFEDAIVISEKVIKEDIFTSIHIEEYTLEVRDTKRGQEELTND
                     IPNVSSEATKDLDENGLVRIGAEVNEGDILIGKITPKGESDPTPEEKLLRAIFGDKAG
                     DVKDASLKAPPSMHGVVIDKKLFSRAVKDKKAKSKEKELLKELEEDFKKKSQELKTKL
                     IEKLLILVNGKVSQGVYSNFKQEIIPKKARFNEKILREIDYTTVNPNRWTLDKEKNEL
                     IKGLINNYNIHYREILGVYNRKKFVATVGDELPNGIVQMAKVYVAKKRKLTVGDKMAG
                     RHGNKGIVAKIVREEDMPFLPDGTPVDIVLNPLGVPSRMNLGQIYETILGWAGKELGL
                     RFSTPIFDGASLDQINEYMGKARLPENGSLYLYDGGTGERFDQPATVGYIYMLKLHHL
                     VDDKMHARSIGPYSLITQQPLGGKAQFGGQRFGEMEVWALEAFGAANVLQEILTVKSD
                     DVAGRAKAYEAIVKGENMPVPNVPESFNVLVHELRGLGLNIAFE"
BASE COUNT          825 a          720 c          649 g         1003 t
ORIGIN      
        1 ctttcaaaac attttcgagg atatcccaga ccacaggatc tttgcgatcg acgattttct
       61 tggccgattt tacggttttt acaattccgc gttcgagtag tttgcgaata ataaaaggtt
      121 tgaaaagttc actggccatt tcctttggaa gcccgcattc attgagcttg agttcggggc
      181 caacaacgat gacggaacgg cctgaataat cgacgcgttt tccaagcaga ttctgacgga
      241 aacgtccttg tttgcctttc aaactgtcgc tcagtgactt gagagcacga ttggattcgg
      301 ttctcacgga gctggccttg cgtgaattat cgaaaagaga atcaacggct tcctgaagca
      361 tgcgtttttc gttacgcaag ataacgtcgg gtgcttttat ctcgatgagt cgtttcagtc
      421 tattgttacg aattatcacc ctgcgataca aatcattgag gtcggaagtg gcaaaacggc
      481 caccatccaa tggaaccaaa ggcctcaatt cgggaggtat tactggaact acctttacca
      541 tcatccattc cggacgattt tcggttcggc ggttggcttc acgaaatgcc tcaaaaactt
      601 gtaaacgttt taaaatgtcc tgtttacgtt gttgagaggt ttcaatgctt gccttgtatc
      661 ttaaatcgaa tgattcctta tcgagatcaa tacgtgaaag aaggtcatat aaggcttcgc
      721 ctcccatttt tgcaatgaat ttatcgggat ggctatcatc gagattctga ttttcagggg
      781 gaaggttttc gatgatcttg aaatagtcct cttcagataa aaaatccatg taagatatgc
      841 cctcgctcga cattatccca ggattgatta ctacataatt ctcgtaataa atgatggatt
      901 ccagcttctt ggtgggtaaa cccaacagag cgccaatttt attgggaaga gacctgaaat
      961 accagatatg agccacaggg acaacaagct gaatatgccc catgcgttcg cgtcgaactt
     1021 ttttctcggt tacttcaaca ccgcaacggt cgcatactat acccttgtaa cggattcttt
     1081 tgtattttcc acaatgacat tcccaatctt ttaccggacc aaagattcgt tcacaaaaca
     1141 agccatctct ctcgggctta taagtgcgat aattaatggt ctctggtttt agaacttcac
     1201 cgtatgatcg ttcgagtatg gattcgggag aagccaaact gatcatgatt tgattgaaat
     1261 tgctgattgg agcagtagga tcttttctaa acgccatatt tgaatttgat tttcagcaaa
     1321 ataaaaattt tatacaaacg cctgagtctt acgccaaatg gcattattca aaggctatat
     1381 tcaggcctaa acctcgaagt tcatgaacca gaacattgaa tgactcgggt acattgggaa
     1441 cgggcatatt ttcacccttg acgatagctt cgtaagcctt ggctcgccct gctacatcgt
     1501 ctgatttaac agtaagaatc tcttgcagaa cattggctgc accaaaggct tcgagtgccc
     1561 aaacctccat ttctccaaac cgttgtcctc caaattgggc cttcccacca agaggttgtt
     1621 gtgtaatgag agaataaggg cctatggaac gagcatgcat cttatcgtca acaagatgat
     1681 gaagcttgag catataaata taacccaccg tggccggttg gtcgaaacgt tcgcctgtac
     1741 caccatcgta tagatacagg cttccgtttt cgggtaatct ggccttcccc atatattcat
     1801 tgatctggtc taatgaagca ccatcgaaaa tgggagttga aaagcgtaag ccaagttctt
     1861 tcccagccca acccaggatg gtttcataaa tctggccaag gttcattcgt gaaggaacgc
     1921 ccaatggatt cagaacaata tctacgggtg tgccatcggg taagaatggc atatcttctt
     1981 ctctcacaat cttggctact atacctttat tcccatgacg gccggccatt ttatcgccta
     2041 cagtcaattt gcgcttcttt gcaacataaa cctttgccat ctgcacaatg ccattgggaa
     2101 gctcgtcacc aactgtggct acaaactttt tccggttata aacaccaaga atttctcggt
     2161 aatgaatatt gtaattattg ataaggcctt tgatgagttc gttcttttct ttatcgaggg
     2221 tccagcgatt gggatttaca gtagtataat cgatttcgcg gagaatcttc tcgttaaacc
     2281 gggccttctt gggtatgatt tcttgtttga aattactgta aacaccttgc gaaacctttc
     2341 cattcactaa gatgagtaat ttctcgatca acttggtttt cagttcttgc gatttctttt
     2401 taaaatcttc ttcgagttct tttaacagtt ctttttcctt acttttggct tttttatctt
     2461 ttaccgcacg gctgaatagt tttttatcga ttaccacacc atgcattgaa ggaggtgcct
     2521 tgagggaagc gtctttcaca tcgcccgctt tatcgccaaa tattgctcta agaagctttt
     2581 cttcgggagt aggatcactt tctcctttgg gagtaatttt tccgatcaga atgtcgcctt
     2641 cgtttacttc ggcgcctatt cttacgagtc cattctcatc gaggtctttt gtagcctcgc
     2701 tgcttacatt gggaatgtcg ttggttagct cttcctgtcc acgtttggta tctctaactt
     2761 ccagagtata ttcttcgatg tggatcgaag taaagatatc ttccttgatt actttttcgg
     2821 aaatcacaat ggcatcttcg aaattatagc ctttccatgg cataaatgct accatgaggt
     2881 ttcggcccag ggccagctct ccattcgaag tagcataacc ctcgcacaaa acttgtcctt
     2941 tcaccacttt gtcgcctttt ctaacaatag gcctaaggtt gatggtagta ttttgattgg
     3001 tacgcttaaa tttaataaga tagtaggttt ttacatcttc atcaaaactg atgagccttt
     3061 ccatatcatt tcgttcataa cggatgacga cgcgggtggc gtcgacatat tccactactc
     3121 cattaccttc ggcgtttatc aaaacacgcg agtcgcgggc tacaaattct tcgatgcctg
     3181 ttcctatgat aggtacc
//
LOCUS       DAORPD010000080         3195 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_5341_length_3195_cov_2.376752, whole genome shotgun sequence.
ACCESSION   DAORPD010000080 DAORPD010000000
VERSION     DAORPD010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5341_length_3195_cov_2.376752"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3195)
                     /locus_tag="PK976_01490"
     CDS             complement(<1..>3195)
                     /locus_tag="PK976_01490"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRC66593.1"
                     /translation="EAQTLLYEAELAFRNKDYASANKLVGHLEHLSFIDPHLHSRLNE
                     LKKKIENQQKQIARYNEAMLEGNDALKAKRYQHASKAFSSALQIKGDDEQAAQGLAQS
                     QQQEKQARILYQQKLAEANQAYQLQKYEQALQLFTEANQILPEGVEAQQKIDEINDIL
                     TYQAEISSQYQKAKAKGDAAFAAGKFDEAMKAYKEAIDINPDETYPKTRIREIQALYA
                     DESSRQQRYKSLINEGDNYLKSGEIEKAYQLFSDALLLKPNDSYGSSKIKSLQPRIDQ
                     LVKNRRSYEQLIHQAAELEKNGEFTKAIQTYQSASKLLPDSTYPQEQIAILTQKFNEQ
                     KQIAIHFEELLKQADQYIVAKNYSQARSFLQQASELQPNNAQVRAKLAQIDELETNFR
                     QLQANYQQAIQKADNLLANKMYNDALVLYQGAIKLKPDETYPKTKIQEIEDLFKQQQA
                     IRNAFDEKLVQADRLLKNNQFAKAKEVLQSALEIFPNDKRALDRIHSIDSTLLVQKSI
                     DEQFEMLLARAGEAENQKDYEQAEKLYREALALKPNHALTSQKIEESSRIRQDIALKE
                     AKYAQTLARGDRYLSEKQYENASKAYAEAIQLKANDLLASRKKAQSDSLLIVEQNRLK
                     LYSQTLSEAQNFFESKQYDSALVYYRQGLKIQPNDKAASARISEIENILGNLRQKRET
                     YESNIKLADQYLATGDLKNALAAYQNALKIYPDEKYPQQKVEEIQLQLEQKAAIEANY
                     RQTIVQANQLAKEGKWQQAKLEYQKAQAINPNEVYPSQKVQQMDSLILAKAEKEKSFN
                     DYVARGNEAFDAQDLDKALGFYQQASQLMPGNPYPQSRIEQIKILKTEKEKAENDAFT
                     LAKANAKNLENAGDYQNALEMYQNALSIKPNNEEVKDRIEALHRIIAQMKANELKYQQ
                     AIAKADSLYKSDNLITALTYYKEASQIKQNETYPLDQILAIETKIKTIEKREQDYARW
                     IKTADSLDKLKNYSQAVLAYENAVQLKPNEEYPKQKITELRNIIQEIATRTEQYHNLI
                     TKADASYKAGKLYEARQFFMQATQVMPDQEYAAN"
BASE COUNT          736 a          585 c          673 g         1201 t
ORIGIN      
        1 ctgtttgcag catattcctg atcgggcatc acctgtgtgg cttgcatgaa aaactgacgt
       61 gcttcatata attttcctgc cttgtatgaa gcatcagctt tggtaataag attgtgatac
      121 tgttcggttc gagtagcaat ttcctgaatt atattccgaa gttctgtaat tttttgcttt
      181 ggatattctt cattcggttt caattgaaca gcattttcat aagcaaggac tgcttgggaa
      241 taatttttta gtttatcgag actatcagcg gtttttatcc atcgagcata atcctgttct
      301 cgcttctcga tggtttttat ttttgtttca atggctaaga tttgatccag tggataagtc
      361 tcattctgct tgatttgact ggcttcctta taatatgtta aagccgttat caaattatct
      421 gatttatata atgaatcagc tttggcaatg gcttgctggt atttgagttc gttagccttc
      481 atctgtgcaa taattctatg aagagcttct attctatctt tcacttcttc attgttcggc
      541 ttgatagata aggcattctg atacatctcg agagcatttt gataatctcc tgcgttttcg
      601 agattctttg cattagcctt ggcaagagta aaggcatcgt tttcggcctt ttctttttcg
      661 gtctttaaaa tcttaatctg ttcgatacgt gattgtggat aaggatttcc gggcataagc
      721 tggcttgctt gctgataaaa accgagcgcc ttatcgagat cctgagcatc aaaggcctca
      781 tttccccttg ctacgtaatc attgaaagac ttttcttttt ctgccttcgc cagaatgagt
      841 gaatccattt gttgaacctt ctgactggga taaacttcat ttggattgat agcttgtgct
      901 ttttgatatt ctaatttagc ttgctgccat ttcccttctt tagccaattg attggcttgg
      961 acaatagttt gcctgtaatt ggcctcaata gcagcttttt gttcgagttg cagttgtatt
     1021 tcttcgactt tctgttgagg atatttttcg tcgggataaa ttttcagcgc attttgatat
     1081 gctgccaagg catttttcaa atctcccgtt gctaagtatt ggtcggcaag tttgatgtta
     1141 gattcataag tttcgcgttt ttgccgcaaa ttgccaagaa tattttcaat ttcggaaata
     1201 cgcgcgctgg ctgctttatc attaggttga attttcaatc cctgccgata gtaaactaaa
     1261 gccgagtcat actgtttgga ttcaaagaaa ttttgggctt ctgaaagcgt ctgactgtaa
     1321 agtttcaggc gattttgctc tactattaat agggaatcgc tctgggcttt ttttcttgaa
     1381 gccaaaagat cgtttgcttt taattgtata gcttctgcat aagctttcga agcattctca
     1441 tattgctttt cgcttaaata tctatcgcct cgagctaaag tttgagcata ctttgcctct
     1501 ttcaaggcaa tatcctgacg aattcgagat gattcctcta ttttttgaga ggtaagagca
     1561 tgattgggct taagtgctaa agcttcgcga tacagtttct cggcttgttc gtaatctttt
     1621 tgattttctg cttcaccagc cctggctaaa agcatttcga attgttcatc aatcgatttt
     1681 tgaactaata aagtgctgtc gatggaatgt attctatcga gggctctttt gtcattggga
     1741 aagatttcga gtgcagattg aagaacttct tttgccttgg caaattgatt atttttcaat
     1801 aatctatctg cttgaaccaa cttttcgtca aaggcgttcc gaatggcttg ctgttgcttg
     1861 aataaatctt cgatttcttg aatttttgtc tttggataag tttcgtcggg tttcaactta
     1921 atagcacctt ggtaaagaac aagggcatcg ttatacatct tattggcaag caagttgtca
     1981 gctttttgta tggcttgttg atagtttgcc tgaagctgtc ggaagtttgt ttcgagttcg
     2041 tcgatttgtg caagtttagc ccttacttgc gcattattag gttgaagttc ggaagcttgc
     2101 tgaaggaagc ttctagcttg agaataattt ttggcgacaa tatattgatc ggcttgtttc
     2161 aaaagttctt cgaaatgaat ggcaatttgt ttttgctcgt tgaatttttg tgtgagtatt
     2221 gcgatttgtt cttggggata ggtggagtcg ggtaggagtt tagaagccga ttggtaagtt
     2281 tgaatggctt tggtgaattc tccatttttt tcgagctcgg ctgcttgatg aatgagttgt
     2341 tcatacgatc tacgattttt taccaactga tcaatccgtg gctgcaatga tttgattttc
     2401 gaagaaccat aactgtcgtt cggtttgagt agaagggcat cggaaaataa ttgatacgct
     2461 ttttctattt cgcccgattt caaataatta tctccttcgt tgatcaatga tttatagcgc
     2521 tgctgcctcg atgattcatc ggcatacaaa gcctggattt cgcgaatacg tgttttaggg
     2581 taagtttcat cgggattgat gtcaatggcc tctttatagg ctttcatagc ttcatcgaac
     2641 tttcctgctg cgaaggctgc atctcctttg gctttggctt tctgatactg tgagctgatt
     2701 tctgcctgat aagtcaggat gtcattaatt tcatcgattt tttgttgggc ttcaacacct
     2761 tcgggtaaaa tttgattagc ttccgtaaat agttggagtg cttgttcata tttttgaagt
     2821 tgatatgcct gattggcttc agcaagcttt tgttgataaa gtatgcgagc ttgtttctct
     2881 tgctgctgac tttgtgcgag cccctgagca gcctgttcat cgtccccctt tatctgcaac
     2941 gcactgctaa atgctttcga agcatgttga tatcgtttgg ctttcagagc atcattccct
     3001 tcaagcatgg cttcattgta tcgagcaatt tgtttttgct ggttttctat ctttttcttt
     3061 aattcattca aacgtgaatg taaatgaggg tcgataaatg aaagatgttc caagtgcccc
     3121 accagtttat tggctgaggc gtaatcctta ttgcgaaatg caagttcagc ttcataaagc
     3181 agagtctgag cttcc
//
LOCUS       DAORPD010000081         3193 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_5350_length_3193_cov_2.414595, whole genome shotgun sequence.
ACCESSION   DAORPD010000081 DAORPD010000000
VERSION     DAORPD010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5350_length_3193_cov_2.414595"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="PK976_01495"
     CDS             complement(<1..679)
                     /locus_tag="PK976_01495"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRC66594.1"
                     /translation="MKYDPSKKIFELLQFGEFGDVNPSITDSATFTFMQAKTMSDTFA
                     GKTEGCFLYSRHWNPSNKYLADALAAMEGTESCWVTASGMAAITITLLHLCNSGDHIV
                     SSLCTYGGTFAFLHNYLAKFNISVSFVDITRPEEVKAAIKPNTKLVYTETMTNPLLQI
                     SDISLLAEIANQHGIPLVVDNTFTPMIVSPALLGAHIVVYSLTKFVNGKNDCVAGAIC
                     ASKDFIQS"
     gene            824..1297
                     /locus_tag="PK976_01500"
     CDS             824..1297
                     /locus_tag="PK976_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HRC66595.1"
                     /translation="MHAEFQLDSLDRKILNILIRNARTPFLEIARECGVSGAAIHQRV
                     HKMERSGIIQNATLKLSPKALGYLTCAFIGIQINLTTTRTHEEVFEKIKQIPEIVECH
                     HITGKYSLLAKIYTRNNEHLKQIIVDKIQSIPEITFTETFISLEEGFMRSFIPEA"
     gene            complement(1357..>3193)
                     /locus_tag="PK976_01505"
     CDS             complement(1357..>3193)
                     /locus_tag="PK976_01505"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HRC66596.1"
                     /translation="IILLAVPGDSAGACFVGFAIGESLGASALRIAGGIFTKIADIGA
                     DLMKIVFSLPEDDPRNPGVIADCTGDNAGDSVGPTADGFETYGVTGVALISFIVLAVT
                     NVEFQSTLIVWIFMMRILMVLTSLLSYGINQAISAKQYKKAKEFDFETPLSRLIWITS
                     GVSIIITFLTSYFLLNDLTTPEGIPLTNLWWQLATIISCGTLAAAIIPELTKSFTSSK
                     SRHVNEIVTASKEGGPSLTILSGMVAGNFSAFWKGLSMVALMFVAYFTTLGFEQPIFG
                     IYPGIFAFGLVAFGFLGMGPVTIAVDSYGPVTDNAQSVFELSRIEQIPGIEKEIEQDF
                     GFTPDFELGKHYLEANDSAGNTFKATAKPVLIGTAVIGATTMIFSIILILAKSGLMAL
                     QLTDPMVILGFIAGGAVIYWFSGASMQAVTTGAHRAVEYIKRNIRLDKEVADVEDSKE
                     VVRICTRYAQYGMWNIFAVIFSLTLAFAFFDPNFFVSYLISIAVFGLFQAIYMANAGG
                     AWDNAKKVVEVDLKQKNTDLHAATVIGDTVGDPYKDTSSVALNPIIKFSTLFGLLAAE
                     ISIHITLAAKNTGTLNYAPWIGAIFLVIGLFFVHRSFYKMRIKKE"
BASE COUNT         1003 a          657 c          677 g          856 t
ORIGIN      
        1 gggattgaat aaaatccttt gatgcacaga tagctccagc cacacaatcg tttttcccgt
       61 tcacgaattt ggtgaggcta tacaccacga tgtgtgcacc taacaaggcg gggctcacta
      121 tcatgggagt aaatgtattg tcgacgacga gtggaatgcc atgttgattg gcgatttcgg
      181 caagtaatga aatatcgcta atttgcaaca acggattcgt catggtttcg gtatagacaa
      241 gctttgtatt gggcttgatg gcggctttta cttcttcggg ccttgtaata tccacaaagc
      301 tgacactaat gttgaatttt gcgagataat tgtggagaaa agcaaaagtt ccgccataag
      361 tgcaaaggct ggagacaatg tgatcgcccg aattgcataa atgaagtaat gttatagtaa
      421 ttgccgccat tccactggct gtaacccagc acgattctgt tccttccatg gctgcgaggg
      481 catctgcaag atatttatta cttggattcc aatggcgaga atacaggaaa cagccttcgg
      541 ttttcccggc aaaggtgtca ctcatagttt tagcctgcat aaaggtaaat gtagcagaat
      601 cggtaatgga ggggttaaca tcgccaaact ccccgaactg aagaagttcg aatattttct
      661 ttgaaggatc gtatttcatg atgatacaat taaagtaagt tttcacaaaa ttatgaaaaa
      721 gacatgtatt cgtgcagatt ttaaaaattt tttatcattt gattaataac tttagcgaaa
      781 tttcgattaa ttttgtaaaa gtgtaagtaa aaaggaagaa aatatgcatg ctgagtttca
      841 actcgatagt ttagaccgca aaattttgaa tattttgatt cgaaatgcca gaactccctt
      901 tctcgaaatt gcgagggaat gcggagtaag cggtgcagcg attcatcaga gagtccataa
      961 aatggaaagg tcaggaatca ttcaaaatgc cactttgaag ctttcaccca aagctctggg
     1021 ttatttaacc tgtgccttca ttgggattca aatcaatctt actactactc gtacacacga
     1081 agaggttttc gagaaaataa aacaaatacc cgaaatcgtg gaatgccatc atattacggg
     1141 aaaatatagt ctgttagcca agatttacac tcgtaataat gaacacttaa aacagattat
     1201 cgtcgataag atacaatcta ttcctgagat tactttcaca gaaactttta tctcgctcga
     1261 agaaggcttc atgcgtagtt tcataccaga agcctgaaat tttaaacgat gattttcaaa
     1321 atcgaattcg cgaaaagcca gaggaaactg attcggttat tcctttttta tgcgcatttt
     1381 atagaatgaa cgatgaacaa aaaataatcc aatcactaga aaaattgcac caatccatgg
     1441 agcataattc aaagtgcctg tatttttcgc agcaagtgta atatgtatgg agatttcggc
     1501 tgcaagcaaa ccaaaaagag tagaaaattt gattatagga ttgagggcca cactcgaggt
     1561 atccttatag gggtcgccca cagtatcacc tatgacggtt gcggcgtgca ggtcggtatt
     1621 tttttgtttc aggtcgactt cgacaacttt tttagcatta tcccaagctc cacctgcatt
     1681 agccatataa attgcctgaa acaatccgaa cacggcgata gatatcaaat aagaaacaaa
     1741 aaagttagga tcgaagaaag cgaaggctaa ggtaagggaa aagatgactg cgaagatatt
     1801 ccacatgcca tattgtgcat atcgagtgca gatgcgcacc acttccttgc tgtcttccac
     1861 atcggctact tctttgtcga ggcgaatgtt acgtttgatg tattctacgg cacgatgggc
     1921 tccagtagtc actgcctgca tcgaagctcc gctgaaccag taaattaccg ctccaccggc
     1981 tataaaaccc aaaataacca taggatcggt aagttgcaaa gccatcaatc cacttttagc
     2041 cagaattaaa atgatggaaa agatcatggt ggtagctccg attactgcag ttccaattaa
     2101 aacaggcttg gccgtagcct tgaaggtatt gccagccgaa tcgttggctt cgagataatg
     2161 cttgccgagt tcgaaatcag gtgtaaaacc aaaatcctgt tcgatttcct tttcgatgcc
     2221 agggatttgt tcgatacgtg aaagctcaaa aacactttgt gcgttgtcgg tcacgggtcc
     2281 atagctatcc actgctatcg tcactggtcc cattcccaaa aatccaaaag ccaccaaacc
     2341 aaaggcaaaa atacctggat aaatcccaaa aataggctgc tcaaagccta aagtagtaaa
     2401 ataggcaaca aacatcagag caaccatgga aaggcctttc cagaatgcgc tgaaattacc
     2461 agccaccatg cccgataaaa tcgtcaatga tggtcctcct tccttcgaag ctgtaacaat
     2521 ttcgtttaca tgccttgatt tgctgcttgt aaaactcttg gtaagttcgg ggataatggc
     2581 tgcagccaaa gttccgcacg aaatgatggt agccagttgc caccataaat ttgttaatgg
     2641 aatgccttcg ggggtagtaa gatcattcaa gaggaaataa ctggtaagga aggtaatgat
     2701 gatagaaacc ccagaggtaa tccagataag cctcgaaagt ggcgtttcga agtcgaattc
     2761 tttggctttt ttatattgtt tcgcactgat agcctgattg attccatagc tcaagagtga
     2821 ggtaaggacc ataagaattc gcatcatgaa tatccatacg atgagggtag attgaaattc
     2881 tacgttggta acggctaaaa cgataaaaga gataagagcc acgccagtta caccataagt
     2941 ttcgaatcca tcggcagtag ggccgacgct gtcgcccgca ttatcgccag tgcagtcggc
     3001 tattacccct ggatttcgag ggtcatcttc cggaaggctg aatacgatct tcatgagatc
     3061 tgcaccaata tcggcgatct tggtaaatat cccaccagct atacgaagag ccgatgctcc
     3121 aagagattca ccaatggcaa aacctacaaa acaagctcct gcactatctc ccggaacagc
     3181 taataaaata atg
//
LOCUS       DAORPD010000082         3182 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_5371_length_3182_cov_2.314679, whole genome shotgun sequence.
ACCESSION   DAORPD010000082 DAORPD010000000
VERSION     DAORPD010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5371_length_3182_cov_2.314679"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="PK976_01510"
     CDS             <1..557
                     /locus_tag="PK976_01510"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRC66597.1"
                     /translation="ILATGYLHEKIEEHFHNIYKYMNIEYSREDEPLGTGGAVKKALE
                     KAEGKQVVVMNGDTMFRVNLGKLYDFTLIMKSDLTIVVRQVNDVTRYGTVEFDETGRI
                     TAFREKGSMEGSGWINGGTYVIHKDFFLRQALPEKFSLEKDLFEKIYLKHPVYAHKCY
                     QYFIDIGIPEDYERAQEEFRAFEF"
     gene            517..1116
                     /locus_tag="PK976_01515"
     CDS             517..1116
                     /locus_tag="PK976_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRC66598.1"
                     /translation="MNERRKSLELLNSDRKFALFLDRDGVINRRIVGGYVTRPAEFEF
                     LPGALEAMSIFSKLFYPIIVVTNQQGIGKGKMTEEQLNEIHSFMKEEIRKAGGEIHAI
                     YHCPDLKVSHSTCRKPRPGMALRARHDFPALNFKHSMMVGDMISDMVFGHNLGMITVL
                     IADEQLARKHPMLIDYRFNSLYDFAMIISKFDVQEPPKK"
     gene            complement(1168..2688)
                     /locus_tag="PK976_01520"
     CDS             complement(1168..2688)
                     /locus_tag="PK976_01520"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRC66599.1"
                     /translation="MDSLLIAIESDSAAISKVMNYQVGRSKIYSLMAIIFQELDEYER
                     SMDYYFKALKLIEKGGQSYDVALIYKGLGGLNLKAGNFGKANEYFNKALIASNKFADP
                     QIKFDIYQEQYEYFKNRKEYSKALEFSVKMIGLSKTAEIPYMTAIALKNSGEIYYLLD
                     EPSLAKAYLYEVIHNESYEEFPNVLSECYAILARLFFDESEYAEAEGYAQKAYEKAFN
                     TSRLSLKANALYELAKSQQAIGKFSEAFDHLKLHLLYKDSLNNINNTHKILQIYSKAE
                     LDKVMAEKKLLENQLTIKMLESSRKGHLLIGSILIILLLSSLALIMVKKYKFEKEVNK
                     KLERQQAIINEQELIIEKEKENQLKIELEHKNRELITHTMLLTQMHEDKVKLIDELQE
                     IYSKVSKVDKDSASVIAGRINALRNSINANTWEDFKTYFENVYTDFYSNLLAAYPNLT
                     PNEKKLCALLRLNLNTKEIAAITSREVRSIESARTRLRKKLGLSPDTNLSIFLSKF"
BASE COUNT         1015 a          565 c          569 g         1033 t
ORIGIN      
        1 ttatattggc tacaggctat ttgcatgaaa aaatcgagga acattttcat aacatataca
       61 agtacatgaa tatagaatac tccagagaag atgaaccctt aggaaccgga ggtgcggtga
      121 aaaaagcatt ggagaaagca gaaggcaaac aagtagtagt aatgaatggt gataccatgt
      181 tcagggtgaa tttgggaaag ttgtatgatt ttaccctcat aatgaaatca gatcttacga
      241 ttgtggtgcg acaggtaaat gatgttacca gatatggaac tgtcgaattt gacgaaacag
      301 gccgtattac agcttttcga gaaaaaggaa gcatggaagg ctcaggttgg attaatggtg
      361 gaacttatgt catccacaag gatttcttcc ttagacaagc cttgcccgaa aagttctcac
      421 tcgaaaaaga cttattcgaa aaaatttatc tcaaacatcc tgtttatgcc cacaaatgtt
      481 accaatactt tatcgacatt ggaattccag aagattatga acgagcgcag gaagagttta
      541 gagcttttga attctgatag aaaatttgcc ctttttctcg acagagacgg tgtcatcaac
      601 cgaaggatag tggggggata tgttacccgg ccagctgaat tcgaattttt acccggagca
      661 ttggaagcaa tgagcatttt ttccaagctg ttctatccca tcatagtagt gactaatcag
      721 caaggcatag gaaaaggcaa gatgacagaa gagcaattaa acgaaataca ttcctttatg
      781 aaagaagaaa tacgcaaggc tggaggtgag atacatgcca tatatcattg ccccgatcta
      841 aaggtttcac actctacttg tcgtaagccg cggcctggaa tggctttacg cgcccgacac
      901 gattttcctg cattaaattt taaacattcg atgatggtgg gcgatatgat aagtgatatg
      961 gttttcggac ataatctggg aatgatcaca gttttaattg cagatgaaca acttgcaagg
     1021 aagcacccta tgttaattga ttatcgattt aacagccttt acgacttcgc tatgattatt
     1081 tcgaaattcg atgttcagga accgccaaaa aagtaaagaa ttaatgtaag taaacggcat
     1141 tctgaatatt tatgctttta aagagagtta aaacttggaa agaaaaatcg aaagattggt
     1201 atcaggagaa agacccaatt tttttctcaa tctggtccgt gccgattcta tgcttcttac
     1261 ttcacgtgaa gtgatagctg caatttcttt tgtgttaaga ttcaatctga gcagagcgca
     1321 taattttttt tcgttagggg ttaagtttgg gtaggcagcc aataaattcg aataaaaatc
     1381 agtataaaca ttttcgaaat aagttttaaa atcttcccat gtgttggcat tgatactatt
     1441 acgtaaagca ttgattctac ctgcaattac agaagcgctg tctttatcca ctttcgacac
     1501 cttgctataa atttcctgaa gttcatctat aagtttaacc ttatcttcat gcatttgagt
     1561 taacagcata gtatgtgtaa ttaactcacg atttttatgt tcgagttcta ttttcaactg
     1621 attttctttt tccttttcga tgatcaattc ttgctcgttg attatggctt gttgtctttc
     1681 gagtttttta tttacctctt tttcgaattt atatttcttc accataataa gtgccaatga
     1741 acttaacaat aagatgatca atatagaacc aataagcaga tgaccttttc tggagctttc
     1801 cagcattttg atggttaact gattttctaa taatttcttt tcggccatta ctttatcgag
     1861 ttcagctttc gaatatattt gcaatatttt atgggtgtta tttatattat tcagagaatc
     1921 cttatataac aaatggagtt ttaaatgatc aaatgcctca ctaaattttc caatggcttg
     1981 ctgactcttg gccaattcat ataaagcgtt agctttcaat gataatctcg aagtattgaa
     2041 tgccttttcg tatgctttct gggcataacc ttctgcttcg gcatactcgc tctcatcgaa
     2101 aaacaatcgg gccaatatag cataacattc agacaataca ttagggaatt cctcataaga
     2161 ctcattgtga ataacttcgt ataaataggc tttagctaat gaaggttcgt ccaataaata
     2221 ataaatttct cccgaatttt ttaaggcaat agccgtcatg taaggaattt ctgcagtttt
     2281 agataaacca atcattttta cagaaaattc taatgctttc gaatattctt tcctattctt
     2341 aaagtattca tattgttcct ggtaaatgtc gaattttatt tgtgggtctg caaatttatt
     2401 gctggcaatc aaggctttat taaaatattc attggctttg ccgaagttgc cagcttttag
     2461 attcaaacct ccaagacctt tatagattaa agcaacatca taagattgcc cgcctttttc
     2521 aattagtttc aaggctttga agtaataatc catcgagcgt tcatattcat ctagctcctg
     2581 aaatatgatg gccatcaaag aatatatctt ggaacgacct acctgataat tcatcacttt
     2641 acttattgca gccgaatccg actctatggc tattaaaagc gaatccaaaa gtttatatga
     2701 ttctttcagc ctgctttgat aaaacatggc acgggctata ttatacgatg aacttatcat
     2761 accatatgga tatgcaaatt ttttcgacaa cgaatcggct ttcatcgaga gaaaaagcat
     2821 tcgttcagga ctattagtca aattttccga tgcgagcaaa agagaatcta ttcttttgat
     2881 tattccgaca tttttcgaag atgcaatctc tttacttgtt tctcccttac cataaggaag
     2941 gaacataccc agcaaaaaaa gaataaatat gagcctcaac ttcaacataa taaaaccggg
     3001 aaaagtacaa aggtaactct tcccggttca tatttcatcg aagatagtgc ttatgataaa
     3061 ctaaagcaaa atgactttct taataactat ttgattttca atttttaact gacaaaaata
     3121 aattcctcgc gataatgatg catttttata ttggaaagaa tgagcaccgg ctgaaatata
     3181 ac
//
LOCUS       DAORPD010000083         3179 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_5379_length_3179_cov_2.282971, whole genome shotgun sequence.
ACCESSION   DAORPD010000083 DAORPD010000000
VERSION     DAORPD010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5379_length_3179_cov_2.282971"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..1082)
                     /gene="gap"
                     /locus_tag="PK976_01525"
     CDS             complement(84..1082)
                     /gene="gap"
                     /locus_tag="PK976_01525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HRC66600.1"
                     /translation="MSVKIGINGLGRIGRLVFRLAIETPGIELVHLNDMMDTPLMSHL
                     IKYDSLHGKLNADLSCQQDHLVVNGSRILVTHCEKPDAIPWKHTGVDIVIDASGKFKT
                     STSLQAHLRNGAKKVILTCPALDNSIERTIVMGVNHKEILPSDRIISNASCTTNCVAI
                     MLKVLQDEFGVKKAFMNTIHPLTNNQNIQDGYHTDYRRARSAINNIIPTTSSAIDAIR
                     IIFPEMKDTFDGFATRVPVAECSFVELIAELDKNVEKYIVNQAFLKHSEGNLNAYLEY
                     CSDPIVSSDIHNNRHSAIFDSLATKVLAGDFIQILGWYDNEFGYSARIIDLIKYIS"
     gene            1600..3096
                     /locus_tag="PK976_01530"
     CDS             1600..3096
                     /locus_tag="PK976_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005863241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HRC66601.1"
                     /translation="MTVNFEFFLFIVSILLFFSIIAGKAGYRFGIPVLLLFLMVGIVA
                     GSDGLGIEFSSARQAQYIGIIALNIILFSGGMDTRFSEIKPVLKPGIAISIGSTILTA
                     FITGFFIYWFTNYIFDSVRFNLVEALLLASIMASTDSASVFSILRSKGLTITQNLRPL
                     LELESGSNDPIAYMLVIICIELLKNSSIHFGQIMLLFVQQIVVGVLVGLIFAKLVVHI
                     INHIHLDYEGLYSVLLLAVVFFLFSFSTILGGNGYLAVYLGGLLIGNHSFVHKRSTLK
                     FFDGLTWLFQIIMFLTLGLLVNPHELLPIAILGIIIGMFMIIVSRPLSVFAILTPFKK
                     ITFKGRVFVSWVGLRGAVPIIFATYPLIHGIEQAQTMFNIVFFITILSLVLQGSSVPM
                     VARWLGLTAQTTKKTKPQLYEFDMEFPDDIKSAISEMTITEQMLKHGRRLMDIRLPDK
                     ILVVMIKRNDKYFIPRGNTHLEPGDVALLIADNEEVLKETYRHLGVPY"
BASE COUNT          915 a          635 c          569 g         1060 t
ORIGIN      
        1 gattccccaa taattttcgg aaagcgataa aaaccacgaa tatttttctg gaagagcatc
       61 tttctgtaag taggtaaaca cggctaagag atatatttaa tcaaatcaat aattcttgca
      121 gagtagccaa attcattatc ataccaaccg agtatctgaa tgaaatcgcc tgccaatact
      181 tttgtagcca gcgaatcgaa tatggctgaa tgacgattat tgtgtatatc actgcttact
      241 attggatcgg aacaatattc caggtaggca ttaagattac cctcagaatg ttttaaaaat
      301 gcttggttta caatatattt ctcgacattt ttatccaact cggcgattaa ttcgacgaat
      361 gaacattctg ccacaggaac acgcgttgca aatccatcga atgtgtcctt catttcaggg
      421 aagataatcc tgattgcatc aatagcgctc gaggtagtcg gaattatatt gttgatagca
      481 gatcttgccc gacggtaatc agtgtgatat ccatcctgaa tgttctgatt gttggtgagg
      541 ggatgaatgg tattcatgaa agcttttttc accccaaatt catcttgtaa aaccttcagc
      601 attattgcta cacaattggt agtacatgaa gcattggata tgattctatc ggagggtaaa
      661 atttctttat gattcacccc cattactatg gttcgctcga tcgaattatc taaggctggg
      721 caagtcaaaa taaccttttt cgctccattg cgcagatgtg cttgcagtga ggtgctggtt
      781 ttaaattttc cgcttgcatc tatcacaata tccactcccg tgtgtttcca tggaatcgca
      841 tccggtttct cacagtgcgt aaccaaaatc ctcgagccat taactaccag atgatcctgc
      901 tgacaactaa gatcagcatt tagctttcca tgcaagctgt cgtattttat taaatgactc
      961 attaagggag tgtccatcat atcattcaag tgaactaatt ctatcccagg ggtttcgatg
     1021 gctaaccgaa aaaccagcct gccgattctt cctaacccat tgatgcctat tttaaccgac
     1081 atgaccgttg agtaatgagt taataaccag aatgtaggat atacaaacgg atcttcagta
     1141 tatcagtttt ttactatatc tgattcagca agcaaattta cgcatttatt ccatgatgac
     1201 acgtataaaa gaacctgtat gccgatggaa gaatttggat ctcttttcat ggaatttaat
     1261 caatggcatc atactaattt aaaataggaa tactttactt ttgaattaat tctattcagc
     1321 taatatccac cttccttatt cgcataaaaa tttcaacggc aattattcga aaagctaaaa
     1381 catcattttt tgaaaactga gggaaacaat ttcaacttac aaagctaaat attgaagata
     1441 attcagtaat tttagctcta cgaaaaattt taattgttgg aagatttttc cttgaatgga
     1501 ataaatttag caatgcagta tctgtctgat tttgccgctc attaaattac cattcatgct
     1561 tgaaagtgaa ttgatatgaa atccacataa ataagttata tgactgtgaa ttttgaattt
     1621 tttcttttta tagtatccat attgctcttt ttcagtatca ttgcagggaa agcaggatac
     1681 cggtttggaa ttccagtatt gctactattc ttaatggtgg gtattgtggc ggggagtgat
     1741 ggtttaggga tagaattcag ttcagcccgt caagcacagt atattggaat cattgctctt
     1801 aacatcattc ttttttcggg aggaatggat acccgttttt ccgagattaa acccgtattg
     1861 aaaccaggaa tagccatatc gattggcagt accattctaa cggcattcat cactggtttt
     1921 tttatttatt ggtttaccaa ttacattttc gattccgtga gattcaatct tgtagaagca
     1981 ttactattgg ccagtattat ggcttcgacc gattcggctt cggttttctc catacttcgt
     2041 tcaaaaggct taaccattac gcaaaacctc cgacccttgc tcgaactcga aagtggcagt
     2101 aacgacccca tagcttatat gctggttatt atctgcattg aattattgaa aaattcgtca
     2161 atacattttg gacaaataat gctattattc gttcaacaga tagttgtcgg agtcctagta
     2221 ggcttgatat tcgccaaact ggtagttcat atcattaacc atatccatct cgattatgaa
     2281 ggcctatatt ctgttttgct acttgctgtg gtatttttcc tgttcagttt ttccactatt
     2341 ttaggtggga atggttattt ggcagtttat cttggaggat tactcattgg gaatcattct
     2401 tttgtacata aacgtagcac tttaaaattc ttcgatggtt taacctggtt atttcaaatc
     2461 atcatgttct tgaccctcgg acttcttgta aaccctcacg aattgctgcc aatagccata
     2521 ctgggcatca tcattggaat gtttatgatt atcgtttcga ggcctctttc tgtttttgct
     2581 atattgacgc ctttcaagaa aattacgttc aaaggtcgtg tattcgtttc gtgggtgggg
     2641 ttgcgaggcg ccgtacctat catttttgcc acttatcccc tcattcatgg aatagaacaa
     2701 gcccaaacca tgttcaacat cgtttttttc attactattc tttcacttgt attgcaaggt
     2761 tcatccgttc ctatggtcgc ccgatggctc ggtttaacgg ctcaaactac caaaaaaacc
     2821 aaaccacaac tctacgagtt tgacatggaa ttcccagatg atatcaaatc ggctatcagt
     2881 gaaatgacga ttaccgaaca aatgctaaaa catggccgac gattgatgga tattcgcttg
     2941 cccgataaaa tactcgtggt tatgataaaa cgtaatgata aatattttat acccagaggc
     3001 aatacacact tagaaccagg cgacgttgct ctgctaatcg ccgacaacga ggaagtttta
     3061 aaagaaacct atcgtcactt gggagttcct tattaattcc tctctcattt tttataatta
     3121 ccctcttttc attaccttaa cccccttcct gttttccgca ttgggacacc ctaaaaatt
//
LOCUS       DAORPD010000084         3171 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_5398_length_3171_cov_2.144095, whole genome shotgun sequence.
ACCESSION   DAORPD010000084 DAORPD010000000
VERSION     DAORPD010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5398_length_3171_cov_2.144095"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(221..970)
                     /gene="dapB"
                     /locus_tag="PK976_01535"
     CDS             complement(221..970)
                     /gene="dapB"
                     /locus_tag="PK976_01535"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HRC66602.1"
                     /translation="MKILISGYGRMGKEVERSALESGHQIIGIYDNAKDWDDKAELIP
                     QCDVVIDFSLPAEAPRNIIRSIDNHKPIVCGTTGWYDQLSFISNYVGQKHGALFYAPN
                     FNLGVNIFMNLSEILADIMKDYPQYEPAITEIHHTRKLDAPSGTAIALAERLIKNLPQ
                     KYEWTKCEEGEQALPHQIPVTSIREDDITGIHTLEFRSKDDRITLTHEAFDRSGFAIG
                     AIMAAQWLLGRKGIFNMNDLLKNHPTITDIA"
     gene            complement(983..2530)
                     /locus_tag="PK976_01540"
     CDS             complement(983..2530)
                     /locus_tag="PK976_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HRC66603.1"
                     /translation="MFTPNDLQQLASKGISKETVEKQIQYFREGFPWAELVRPATVGD
                     GILRFDDAQISELENYYEKNRHGLDILKFVPASGAASRMFKHLYEFREQYDGSEDALA
                     KLESDRGLQSVYTFIHRIRDFAFSEQLENIFHQKGTSIDKVLEKKDYNLLISCVLEPW
                     GLNYGNLPKGLILFHKYPEGPRTSIEEHLVEGAQHARKNNDEARIHFTVSPEHKEKFE
                     ELITRKAPFYEKKLDVHFRIEYSIQKSSTDTIAVDLNNQPFRNKDGSLLFRPGGHGAL
                     LENLNELEGEIVFIKNIDNIIGDRLRSETIKYKKVIGGLLLKIRNRVDDFLLHLEHTA
                     VDNELLNEVEKFASETLFIYLPDEYKNMSNHAKAAWWKDKLDRPVRVCGMVKNIGEPG
                     GGPFLVKNQSGEISLQIVESSQINLNDPKQKAIMAQSTHFNPVDLVCSLTDYKGRRFH
                     LPNYVDPSTGFISIKSKDDRDLKALEHPGLWNGAMAKWLTVFVETPLITFNPVKTIND
                     LLRPEHQ"
     gene            complement(2545..>3171)
                     /locus_tag="PK976_01545"
     CDS             complement(2545..>3171)
                     /locus_tag="PK976_01545"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRC66604.1"
                     /translation="DFLYKVEKEILQSGKEAIAFPMDVSNKDNWNEFIHKVIESWQKI
                     DILINNAGIMPLSFIRNLRVDEWERMIDVNLKGVLFGTAAVLPYMRSQGYGHIVNISS
                     TAGRWLFDGGAVYCATKFAVNAFSEGFRQEITSTENIRMTLIEPGITATNLPSTINDP
                     DIRASSEDNPPIMPLQPSDIAAAVIYTLTQPPQVNVNEILVMPTQQKI"
BASE COUNT          856 a          631 c          668 g         1016 t
ORIGIN      
        1 cgaattggcc aatgcaactt gtcctgaaaa ggactttaag aacacagaca tgagttaatg
       61 ttcagcatac tgggctggac caaactcgca ggagaaactc gcaacaccaa aggtgataat
      121 tttttggtac tatttatagg agaaagatac ctcgaaaatt ttaaaaaaca aagagatttt
      181 aaaaaacgga ggatggtgat tggtggatgg aggttggtga ttaggcgatg tcggttatcg
      241 ttggatgatt tttaaggagg tcgttcatat tgaagatgcc tttgcgacct aaaagccatt
      301 gtgcggccat gatggctcca atggcaaatc cactgcggtc gaaagcttcg tgggtgaggg
      361 ttatcctgtc gtctttcgaa cgaaattcca gtgtgtgaat gcccgtgatg tcatcttcac
      421 gtatagaagt aacaggaatt tgatgtggca gggcttgctc gccttcttcg cattttgtcc
      481 attcatattt ttgcggcaag tttttgatta atctttcggc caatgcaatg gctgttccgc
      541 ttggggcatc gagtttccgg gtatgatgga tttcggtaat agcaggttca tattggggat
      601 aatctttcat aatatctgcc aaaatctcgc ttaagttcat aaaaatatta acccccaaat
      661 tgaagttagg cgcataaaac aaggctccat gtttttgtcc aacgtagttg cttatgaacg
      721 acaattggtc gtaccatccc gttgttccac aaacaatggg tttatgatta tcgatgctac
      781 gaatgatgtt tcgtggtgct tcggcaggaa gactaaaatc tatgacgaca tcgcactgtg
      841 gaatgagttc ggctttatcg tcccaatctt ttgcattgtc gtaaattcca ataatttgat
      901 ggcctgattc cagggcagat cgttcgactt cttttcccat tcggccatag cctgaaatta
      961 atattttcat aatttttctt gtttattgat gttcgggacg tagaagatcg ttgatagttt
     1021 tcacgggatt gaaggtaatg agaggagttt cgacgaaaac ggtaagccat tttgccatgg
     1081 ctccattcca aagacccgga tgttccaagg ctttcaagtc gcggtcgtct tttgacttga
     1141 tagagatgaa gcccgtggaa ggatcgacat aattgggcaa atgaaatcga cgacctttat
     1201 aatcggtgag actacaaacc agatcgacag gattgaaatg agtggattgg gccattatgg
     1261 ctttttgctt cggatcattt aaattgattt gtgaagattc aacaatctgt aaactgattt
     1321 cgcccgattg atttttcacc aggaaaggtc cgcccccagg ttctccaatg ttttttacca
     1381 tgccacaaac ccttacaggg cgatcgagct tatccttcca ccatgcagct ttcgcatgat
     1441 tggacatgtt tttgtattca tcgggtaaat aaataaataa agtctcggaa gcaaattttt
     1501 cgacttcatt taataactca ttatctacgg ctgtatgttc gaggtgtagc aaaaaatcat
     1561 cgactcgatt acgtatctta agtaaaaggc ctcctatgac tttcttatat ttaatagtct
     1621 cacttctgag cctgtcgcca ataatgttgt cgatattttt gataaaaacg atttcacctt
     1681 ccagttcgtt caggttttcg agcaaggcgc catgaccacc tggtctgaag agtaagcttc
     1741 catccttatt tcggaaaggt tgattgttca aatcgacggc gatggtatcc gtagatgact
     1801 tctggatgga atattctatt ctaaagtgca catccagttt tttttcataa aaaggggctt
     1861 ttcttgtgat gagttcttcg aatttttctt tatgctcggg cgaaaccgtg aaatgtattc
     1921 ttgcctcatc attgtttttc cttgcgtgtt gtgcgccttc tactaaatgt tcttctatgc
     1981 ttgtacgtgg cccttcggga tatttgtgaa aaagaataag acccttaggt agatttccat
     2041 aattgagtcc ccaaggttcg agtacacatg aaataagcaa attgtaatct ttcttttcta
     2101 aaactttgtc gatagacgtg cctttctgat gaaaaatgtt ttccaactgc tcggaaaatg
     2161 caaaatcgcg aatccgatga atgaaagtat aaaccgattg caagcctcta tcgctttcta
     2221 attttgccag tgcatcctcc gaaccatcat attgttccct gaattcgtag agatgtttaa
     2281 acatccgcga agctgcgcca gatgcaggta caaacttgag aatatccaat ccatgacgat
     2341 tcttttcata ataattttca agttcggaaa tttgcgcatc atcgaacctg agaattccat
     2401 ctccaaccgt tgcaggtctt acaagttcag cccatggaaa cccttccctg aaatattgaa
     2461 tctgtttttc aacggtttct ttgctaatac ctttggaggc taattgttgt aaatcgttgg
     2521 gtgtaaacat cgtttgaact gtttttaaat tttttgttgt gtgggcatga caagaatttc
     2581 gttcacattg acttgaggag gttgtgttag cgtataaatg actgcagctg caatatcact
     2641 gggttgcaaa ggcataatgg gaggattgtc ttcactgctg gctctgatgt cgggatcgtt
     2701 gattgtgctg ggaagattag tggccgtaat acctggttct attagagtca ttctgatgtt
     2761 ttcggtcgat gtaatttcct gcctgaatcc ttccgaaaaa gcattgacgg caaattttgt
     2821 ggcacagtaa accgcacccc catcgaataa ccaacgacct gcagttgacg aaatattaac
     2881 gatatggcca taaccctgag aacgcatata tggtagcaca gctgccgttc caaacaatac
     2941 tcccttcaaa ttcacatcga tcattctttc ccactcgtct acccgcaaat ttctaataaa
     3001 tgataagggc ataatcccgg cattgttgat gagtatatct attttttgcc agctttcgat
     3061 gactttatgg ataaattcat tccaattgtc cttattgctc acatccatcg gaaaggcaat
     3121 agcttcttta cctgattgta gaatttcctt ttctacttta tagagaaaat c
//
LOCUS       DAORPD010000085         3157 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_5425_length_3157_cov_2.495809, whole genome shotgun sequence.
ACCESSION   DAORPD010000085 DAORPD010000000
VERSION     DAORPD010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3157
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5425_length_3157_cov_2.495809"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1289)
                     /locus_tag="PK976_01550"
     CDS             complement(<1..1289)
                     /locus_tag="PK976_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66605.1"
                     /translation="MITPSVSNDQTTSFTERDLQQMFDRFDFTDETRNIVEQENLLPL
                     GLIRRLHCAFPKYSAFREERPFMFKQKRQYIGLEGFREIVRILQSNGVKMDHLEEREL
                     FIKVYRFMATRHVLNIINWDNPSTDSLFQLVFPQPGMIRKGIVDAYFAAGTDAEKEKI
                     ISDYIRHTHPHDGKQLLNKPVFENEEGKMEVLEGCQHKYPQCELIFDVQTQNCFAFCT
                     YCFRHAQVRGDIDMFLQRDISQIHRYLRKHKEVTDILITGGDAGYLKADRFESYITPI
                     IEDPELNHIRTVRIGTRVLTYFPEMILTHDYENMLNLYRRLIDNGIQILFMSHFSTPR
                     EIMNPTTVAAIRRLRNYGVTMKSQSPIMNHISLFTDENGKVDIDRSAQNWIDLGNLLA
                     MLGVGFHSMYMARPTGEHHYFTAPLADINNIFQKVYRS"
     gene            complement(1821..>3157)
                     /locus_tag="PK976_01555"
     CDS             complement(1821..>3157)
                     /locus_tag="PK976_01555"
                     /inference="COORDINATES: protein motif:HMM:NF024152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HRC66606.1"
                     /translation="AQYWAQNNYTDEDRYNIRQNVNDGYWRTIYTIMVDLNEIIELNQ
                     NPDKKDLLSAYGANENQIASARILRAWVFQHLTDLYGDIPYFEALKAKSDRVFLPPYD
                     KQSDIYPAIIQELTEAAAQIDVTKAGWTQGDIIYKGDMTKWKKFANSLKLRMALRMKK
                     ADPTNAQKYIDEAIAGGVFTSNGDVAAFKYLPSAPNTAPMYSAYYIDNRTDFAVTAQF
                     VDLLKGIPVGGAQKPNPFAGLEDPRLPIFADPVKDSTMLGPGYQGMPYGMPDEITKAI
                     GAQGSSWPGELILAPDFATILMDYAEVEFILSEINGWDQTHYINGVKASLDYWGVDAA
                     TRDAFLAALPPASQETVLTQKYIALYTQGYEGWSEYRRTGYPKTLVHPGEVTWIDLSS
                     NPVKFEALTGDDIPRRLTYPQEEQKVNVTNYKAAVAQFGEDVFSTRMWWDKP"
BASE COUNT          940 a          619 c          636 g          962 t
ORIGIN      
        1 gaacgataaa ctttctgaaa aatgttattg atatcggcca aaggagctgt gaaataatga
       61 tgttcgcccg ttggacgagc catatacata gaatggaagc caacacccag catagccaat
      121 aaattgccca aatctatcca attttgagcc gaacggtcta tatccacttt cccattttcg
      181 tcggtaaaca gactaatatg attcatgatg gggctttggc tcttcatagt tactccataa
      241 ttgcgcaacc gacgaatagc agccacagtc gttggattca tgatttcacg gggagtagaa
      301 aaatgagaca tgaaaagaat ctgtatccca ttgtctatca atcggcgata taaattcaac
      361 atattttcgt aatcgtgagt aagaatcatt tcaggaaaat aagtaagcac gcgtgtgcca
      421 atacgtaccg tccgaatgtg atttaattca gggtcttcaa taattggcgt aatgtagctt
      481 tcgaaacgat cggctttcag atatccggcg tctcctccgg taatcaaaat atcagtaact
      541 tctttgtgtt tgcgtaaata acgatgaatc tgcgaaatat ctctctgtag aaacatgtca
      601 atatcgcctc taacctgagc atgacggaaa caataagtac agaaagcaaa gcagttttgg
      661 gtttgtacat caaaaatcaa ttcacactgt ggatatttgt gttggcatcc ctcgagcact
      721 tccattttgc cctcttcgtt ttcgaataca ggcttattaa gcaattgctt accatcatgt
      781 gggtgagtat ggcgaatgta gtcagagatt attttttcct tttcggcatc ggtacctgct
      841 gcgaagtatg catctactat tcccttccta atcatccccg gttgaggaaa aaccagttga
      901 aacagggaat ccgtggaagg attatcccag ttgatgatat tgagcacatg gcgagtggcc
      961 ataaaacgat ataccttgat gaacaattcc ctttcttcga gatgatccat tttaactccg
     1021 ttcgactgaa gaatcctgac gatttctcta aacccctcaa gccctatata ttgccttttc
     1081 tgtttgaaca taaaaggtct ctcttctctg aaagcagagt actttgggaa agcacaatga
     1141 agtcggcgaa ttaaacctaa cggaagtaaa ttttcttgct ctacgatatt tcgggtttca
     1201 tcggtaaaat cgaacctatc gaacatttgt tgtaagtctc tttctgtaaa agaagtagtt
     1261 tgatcattag aaacagatgg agtaatcatt ttatattctg aatttatgtt gattaatagc
     1321 taacgaattt tgtataaaca tcgtgcaatc cataggttat gatgaattaa agagctaatc
     1381 gatcagtttg caattcactt ctcatgtctt tcacaatgaa tttaccttta tcattttatc
     1441 taaattttac acactctaaa attttatttt tctttgttga gaaatgaaaa acagatcaca
     1501 tataattgtg aacaaatcga agcaaaatta atatttatag gaaaatccaa caatgattaa
     1561 agaaaattcg aaacattatc gaaaataaaa tggcttaacg gataatgtag tagcatgtaa
     1621 aacgaacatg gaatcctgag gttttatttg agttctatga gaaattcaag aaaagatatt
     1681 aggatgaatg gatagttgat acgctaggat ttcgtttctt actaattatt aattaatgaa
     1741 ttatagaaga taattaatac gagagggcgg cccaaagagc cgccctctcg attaataaaa
     1801 gttgttgttc ctttatggaa tcatggttta tcccaccaca tgcgggttga gaagacatct
     1861 tcaccaaatt gagcaacagc ggctttataa ttggtgacat ttactttctg ttcttcctgt
     1921 ggataagtta gacggcgtgg aatgtcatca cctgtaaggg cttcaaattt aacggggtta
     1981 ctgctaagat cgatccaagt aacttcacct ggatgaacga gggtttttgg ataacctgtt
     2041 ctacggtatt cactccagcc ttcataacct tgggtataaa gggcaatata tttctgggtg
     2101 aggactgttt cttgtgaagc cggcgggagg gctgccaaga aggcatcgcg tgtagcggca
     2161 tccactcccc aatagtcgag tgaagccttt actccgttga tgtagtgagt ctgatcccat
     2221 ccattgattt cgcttaaaat aaattcgact tcagcataat ccattaagat tgtagcgaaa
     2281 tcgggggcaa ggatgagttc ccctggccat gaagaaccct gagctcctat agccttggtg
     2341 atttcgtcag gcattccata aggcattccc tgataaccgg gtccaagcat agtagaatct
     2401 ttcacgggat cagcaaaaat tggcagacga ggatcttcca acccagcaaa aggattgggt
     2461 ttttgagcac cacctacagg gattccctta agtaaatcta caaactgcgc tgtaaccgca
     2521 aaatcggtac gattatcgat gtaataggca gaatacatgg gtgcagtatt gggtgcagat
     2581 ggaagatatt taaaggctgc aacatcccca ttgctggtaa atactcctcc cgcaatcgct
     2641 tcatctatgt acttctgtgc attggtagga tcggcttttt tcattctcaa cgccatacgt
     2701 agcttcaaac tattggcaaa ttttttccat tttgtcatat cacccttata aataatatca
     2761 ccctgcgtcc aaccagcttt tgtaacatcg atttgtgcgg cagcctcggt gagttcttga
     2821 ataatagcag gatagatatc actctgcttg tcataaggtg gtagaaatac tctatctgat
     2881 tttgctttta gggcttcaaa ataggggata tctccataaa ggtcggtaag atgttggaac
     2941 acccaagcgc ggaggattcg tgccgaagca atttgatttt cattggctcc ataagccgaa
     3001 agcagatcct ttttatcggg attttggttc aactcgatga tttcgttgag atctaccata
     3061 atggtgtaaa ttgttctcca atagccatcg tttacattct gacggatatt gtaacgatct
     3121 tcatcggtgt agttattttg agcccaatat tgagccc
//
LOCUS       DAORPD010000086         3149 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_5442_length_3149_cov_2.052036, whole genome shotgun sequence.
ACCESSION   DAORPD010000086 DAORPD010000000
VERSION     DAORPD010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5442_length_3149_cov_2.052036"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2637
                     /gene="gcvP"
                     /locus_tag="PK976_01560"
     CDS             <1..2637
                     /gene="gcvP"
                     /locus_tag="PK976_01560"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HRC66607.1"
                     /translation="GYYNTILPGVIQRNILENPGWYTSYTPYQAEISQGRLEALLIFQ
                     TMVADLTGLPISNSSLLDEATAAAEAMIMAYNCRSRSKMKTNANKFFVSENLFPQSIA
                     LLQTRSVPLGIELVIGNHDKVQLDDGFFGGIVQYPDGFGQINNYRAFAEKIHSVEALV
                     VVAADILSLVLLTPPGEWGADIVIGSTQRFGIPMSYGGPHAAFLASKEEFKRSIPGRI
                     IGISIDTQGRPSLRMALQTREQHIKREKATSNICTAQALLAIMAGMYTVYHGPEGLHK
                     IARHINILTGVLANEASKYGYNQLNSAFFDTVRLRLPEQVNIKTIKNLALKRQFNLRY
                     IDDQTLGISLDETTSLKEVNQILEILSLAANRTFQEFKCNPVECENIVTFPQEFIRRS
                     AYLTHPVFNSYHSETAMMRYLKKLENRDLALNRTMIPLGSCTMKLTAGVEMFPLSWSE
                     FNGLHPFVPADQAEGYHLMINELEALLKEITGFSAFTFQPNSGANGEYTGLMIIRKYH
                     ESRGEGHRNVCLIPSSAHGTNPASAHFAGMDIVVVKCDENGNVDIACLREKAVQYKDH
                     LAALMITYPSTHGVFEEEILEIVNIIHQNGGQVYMDGANMNAQVGLTSPGRIGADVCH
                     LNLHKTFAIPHGGGGPGVGPVGVASHLIPFLPQHPIVKTGGQQGISPVAAAPYGSALV
                     LTISYAYMKLLGRDGLTNASKYAILNANYLKARLENHYKILYTGKNGRVAHEMILDCN
                     KFQLSADVSVIDIAKRLIDYGFHAPTVAFPVHGTLMVEPTESEPLEELDRLVDALIAI
                     RKEIASIENHQADRQNNLLKNAPHTAAMVVNSQWNYPYTREKAAFPLPGMETDKYFPP
                     VSRIDDAYGDRNLVCTYQTFDF"
     gene            complement(2822..>3149)
                     /locus_tag="PK976_01565"
     CDS             complement(2822..>3149)
                     /locus_tag="PK976_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HRC66608.1"
                     /translation="INKNLWSSSFVCWTAGISLLLFAVFYFIIDVMEYKKWAFPLVVI
                     GMNSITIYLAQRVFYLGYTSDFLFGGFAALFNQTWQPVIIHLSYALTCWLLLYWLYKK
                     KIFLKI"
BASE COUNT          944 a          721 c          638 g          846 t
ORIGIN      
        1 ggatattaca atactatcct gcccggtgta attcagcgta atattctcga aaatccggga
       61 tggtacactt cttatacacc ttatcaagcc gagatttcgc aagggcgttt agaagcattg
      121 ctcatttttc agaccatggt agccgattta acgggtttgc ccatcagcaa cagttcattg
      181 ctcgatgaag ccaccgctgc tgctgaggct atgattatgg catacaactg ccgctcacgt
      241 tcaaaaatga agaccaatgc aaacaagttt tttgtttcgg aaaacctttt ccctcaaagt
      301 attgctcttc tacaaacaag gtcagttcca ttgggaatag aactggtcat tggcaatcac
      361 gataaggtcc aactggatga tggttttttt ggtggcatag ttcaatatcc cgatggattt
      421 ggacaaatta acaattacag agcttttgca gagaagatac attccgtgga agcgctggtg
      481 gttgttgcag cagatattct ttcattggtt ctgctaacgc ccccaggaga atggggagcc
      541 gatattgtga ttggttcgac ccagcgtttt ggaattccca tgagttatgg tgggccacat
      601 gctgcatttt tagcatcgaa agaagagttc aaacgctcga taccgggtcg aattatcggc
      661 atatcaatag atacacaagg gcgtcctagc ttacgcatgg ctttgcaaac tcgcgagcaa
      721 cacatcaaaa gagaaaaggc cacttccaac atctgcaccg cgcaagctct tcttgccatc
      781 atggcaggta tgtacaccgt atatcatggg ccagaaggcc tgcataaaat tgcacgccat
      841 atcaatatcc ttactggcgt tttggccaat gaagcttcca aatatggata caaccaactc
      901 aattcggcct tcttcgatac tgttcgactc cgccttcctg aacaagtaaa catcaaaacc
      961 attaaaaacc ttgcgttgaa gcgccaattc aatttgcgct atatcgatga tcaaacactt
     1021 ggcattagcc tcgatgaaac cacatccttg aaggaagtca atcagatact cgaaattctt
     1081 tccttagctg caaatcgaac ttttcaggaa tttaaatgca atccggtcga atgtgaaaat
     1141 atagttactt tccctcagga atttattcgt cgaagtgcat acctcactca tcctgttttc
     1201 aattcttacc attccgaaac tgccatgatg agatatttaa aaaaactcga gaatcgggat
     1261 ttagctctca atcgaacgat gattccccta ggaagctgca ccatgaaact cactgctggt
     1321 gttgaaatgt tccctttgag ctggtccgaa ttcaatggtt tgcatccttt tgtacccgcc
     1381 gaccaagccg aaggttatca tttgatgatc aatgaattgg aagctctcct aaaggaaatc
     1441 acgggatttt cggcttttac ttttcaaccc aattcaggag ctaacggaga atataccgga
     1501 ttgatgatca ttcggaaata tcatgaaagc agaggagaag gccatcgtaa tgtgtgtctc
     1561 attccctcat cggctcacgg aaccaatcct gcctcggctc actttgccgg aatggatatt
     1621 gtggttgtta aatgcgacga aaatggaaac gtcgatatcg cttgtctgcg tgaaaaagcc
     1681 gttcaatata aagatcatct agcagctttg atgatcactt acccttccac gcatggagtt
     1741 ttcgaagaag aaatcctcga aatcgtgaat atcattcatc aaaacggagg tcaggtttat
     1801 atggatggag ccaatatgaa tgcacaggta ggattgacga gtccaggacg cataggagca
     1861 gatgtttgcc atttgaacct tcacaagact tttgctattc ctcatggagg aggagggcct
     1921 ggagttggac cagtaggagt cgccagccat ctgattcctt tcttgcctca gcatcctatc
     1981 gtaaaaactg gtggccagca agggatatcg cccgtagcag ctgcacctta tggtagcgct
     2041 cttgttctta ccatatctta tgcttacatg aaacttttag gaagagacgg acttacgaat
     2101 gccagtaaat atgccattct caatgcaaac tatttaaagg ctcgcctcga aaatcattat
     2161 aaaatcctct atacgggcaa gaatggtcgt gtagcccacg aaatgattct cgattgtaat
     2221 aaatttcagc tttcagccga tgtatcggtc atcgatattg ccaagcgttt gatcgattat
     2281 ggctttcacg ccccaaccgt tgctttccca gtacatggta cactaatggt agaacccacc
     2341 gagagcgaac ctctcgaaga actcgaccga ctggtggatg cattaattgc tatcagaaaa
     2401 gaaattgcca gtatagaaaa ccatcaggca gaccgccaga acaacctctt gaaaaatgct
     2461 ccccataccg cggccatggt tgtaaattca caatggaatt atccttatac ccgcgaaaag
     2521 gcggccttcc ccttgcccgg aatggaaacc gataaatatt tcccccctgt gagccgcatc
     2581 gacgatgctt atggagatag aaacttggtt tgcacctatc agactttcga cttctaaaga
     2641 accctctgaa tttctctaca aaaggctttc aacccaccca aatattacag aataaagatt
     2701 ttgatgggtt gaaactacct cttattatgt gacatcaagc gacatcaagc aaaaatccta
     2761 tcattcaaaa aagcctaata attctatatc tcatggaata gccgggaccg aaatgttaca
     2821 ctcatatctt caaaaaaatc ttcttcttgt acaaccaata caatagaagc caacaggtca
     2881 atgcatacga cagatggata atgactggct gccatgtttg attgaagagg gctgcaaatc
     2941 ctccgaaaag aaaatcggaa gtatatccca aataaaatac tctttgagcc agatatatgg
     3001 taatggaatt cattccgatg accaccaatg ggaaagccca tttcttatat tccatcacat
     3061 cgatgatgaa atagaaaacc gcaaataata aaagactaat gccagccgtc cagcaaacaa
     3121 atgaactcga ccacaggttt ttattgatg
//
LOCUS       DAORPD010000087         3140 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_5464_length_3140_cov_2.503404, whole genome shotgun sequence.
ACCESSION   DAORPD010000087 DAORPD010000000
VERSION     DAORPD010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3140
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5464_length_3140_cov_2.503404"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..675
                     /locus_tag="PK976_01570"
     CDS             91..675
                     /locus_tag="PK976_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007214725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRC66609.1"
                     /translation="MPKACFYLNNSLANSINEHVDISVARCYQCGKCSAGCPIVEFMD
                     YSPSLVLRYLQTELPENDDAVLRSKTIWYCLSCEMCIARCPMEVDIPVIMDFLRIESI
                     RLRKTHKEVKNIIRFHKAFLNTIKRNGRLHEMGLVLDYKTRSLDLLQDLALSPGMFKR
                     GKLRILPEQVKNRKEIKHIFTIGSNKRKNKSIKN"
     gene            679..1584
                     /locus_tag="PK976_01575"
     CDS             679..1584
                     /locus_tag="PK976_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007214724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HRC66610.1"
                     /translation="MKLGFYPGCSLEGSSREYNESTIAIAEKFDIELVQIPDWNCCGA
                     TAAHNLDKKLSLLLPARNLVLAEEGGLKDVVVPCAACFSRLSTTHHELLLDEELRHQV
                     DNIFGKHYKGSIRILNVIQFIHEYILEKLDEKIVNEFRNKVACYYGCLLVRPHKILNF
                     DRTEDPQTMDLVMKKLGAEPIDWAYKTECCGAGFSVSFTDAVSKLSGKIVEDAIHRGA
                     EAIIVACPMCQSNLDMRRCGIDKYLGKRTNIPVLYITQAIGLALGMNMKQLGLHRHFV
                     PVNFDKFKLIKKQTKVEEPQKMEEK"
     gene            1584..>3140
                     /locus_tag="PK976_01580"
     CDS             1584..>3140
                     /locus_tag="PK976_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007217450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HRC66611.1"
                     /translation="MARIGVFICHCGENIGETVDCTKLAEVTSRFPGVVKSVDYKYMC
                     SEPGQTLIKEAIKGNRLTGVVVAACSPRMHESTFRKTCSEAGLNPFLCEIANLREHCS
                     WVHEKSEEATNKAIDIVRALVEKVKLNEPLYPIKVPVTKTALVIGGGVAGIQAALDIA
                     NGGHKVILVEKNPSIGGHMSQLSETFPTLDCSQCILTPRMVELAQHPNITLYSYAELE
                     SLDGFIGNFKVKIRKKAKSIDENLCTGCGLCTTKCPVKKIPNEFDENLSYRKAIYVPF
                     PQAVPNKPVIDRENCTYYLKGKCKICERFCPTQAIRFDQQDEIIEVEAGAIVLATGFD
                     IKRADFFPEYGFGKYKDVIDGLQFERIASASGPTLGEIRRLSDGKIPKKVVFIACAGS
                     RDPAKGIEYCSKICCMYTAKHAILYKHKIHDGQAYVFYMDIRAGGKNYDEFTRRAIEE
                     EGAKYIRGRVSRIYERDGKLIVVGADTLMNARPVVIEADMVVLATAGVASPGSEELAQ
                     KLHVAYDQYKF"
BASE COUNT          994 a          553 c          692 g          901 t
ORIGIN      
        1 cccttcatct attccgactt agaaatgtta taaaatagtt gaataattat ctcaaactcg
       61 ttatatattt gcggtataaa atttttgact atgcctaagg cgtgttttta tttaaataat
      121 tcgttggcaa acagcatcaa tgaacatgta gatatttcgg ttgcaaggtg ttatcaatgc
      181 gggaaatgct ctgcgggctg tcctattgtt gaatttatgg attattcacc gagtttggtg
      241 ttacgatatc ttcaaactga attacccgaa aacgatgatg ctgtcttgcg ttccaagaca
      301 atttggtatt gtttgtcttg tgaaatgtgt attgcacgtt gtccgatgga agtggacatt
      361 cctgttatta tggatttttt aagaatcgaa tcgatacgat taagaaaaac ccataaagaa
      421 gtaaagaaca tcatcagatt tcataaagcc tttctgaaca ccattaaacg taatggaagg
      481 ctgcacgaaa tggggttagt attggattac aagacgaggt cgttagatct gcttcaggat
      541 ctggctttat cgcccggtat gttcaaaaga ggaaagttaa gaatattgcc cgaacaggtc
      601 aagaatcgca aagaaattaa acatattttt accatagggt cgaataaacg aaagaataaa
      661 tcgataaaga attaacgcat gaaacttgga ttttatcccg gatgttcact ggagggatca
      721 tcgagagaat ataacgagtc gacgatagcc attgccgaaa aattcgatat agaattagtt
      781 caaattcccg attggaattg ctgtggtgct acagcggctc ataatttaga taaaaaatta
      841 tctttattgc ttccggcacg taaccttgtc cttgccgagg aagggggatt gaaagatgtt
      901 gttgtccctt gtgcagcatg ctttagccga ttgagtacca cacaccatga attattactt
      961 gatgaagaat tgcggcatca ggtcgataat attttcggga aacattataa gggaagcatc
     1021 cgcattttaa atgtcattca atttattcat gaatacattt tagagaagct ggatgaaaag
     1081 attgtaaacg aatttcggaa taaagtagct tgctattatg ggtgcttact ggtacgtcct
     1141 cataaaatac tgaattttga cagaaccgaa gatcctcaaa ctatggattt ggtgatgaaa
     1201 aaattagggg ctgaacctat tgactgggca tataaaacgg aatgctgtgg tgcaggattt
     1261 tctgtttcat ttaccgatgc cgtgtcgaag ctatcgggaa agatcgttga agacgctatt
     1321 cacagaggcg ccgaagccat cattgttgcg tgcccaatgt gccaatccaa tctcgatatg
     1381 agacgatgtg gcatcgataa atatttaggg aaaagaacca atatccctgt attatatatt
     1441 actcaagcta ttggtttggc tttaggaatg aatatgaaac agcttggatt acatcgtcat
     1501 ttcgttcctg ttaactttga taaattcaag ttaattaaga aacaaaccaa agtcgaagaa
     1561 ccacaaaaaa tggaggaaaa ataatggcac gaataggagt ttttatctgt cattgtggcg
     1621 aaaacattgg cgaaaccgtc gattgcacca agctggcaga agtcacttct cgttttccgg
     1681 gcgtagttaa aagtgtagat tataaatata tgtgttccga accgggtcaa accttgatca
     1741 aggaagctat aaagggaaat agactcaccg gagttgtggt agccgcctgt tcaccccgaa
     1801 tgcatgaatc tacttttcgt aagacttgtt ctgaagccgg attgaatcct tttttatgcg
     1861 aaatagcaaa ccttagggag cattgttcat gggtacacga aaaaagtgaa gaagcaacca
     1921 acaaggctat tgatatagtt cgtgcactgg tagaaaaagt caaactaaac gaaccactct
     1981 atcctataaa agttcctgtt accaaaacag ctcttgtgat tggaggtggt gttgctggaa
     2041 tccaggcagc tctggatatc gccaatggtg gtcataaggt tattttagtc gaaaaaaatc
     2101 catctatcgg tgggcatatg tcacaattgt ctgaaacatt tcccacactc gattgctctc
     2161 aatgtatatt aacccctcga atggttgaat tagctcagca tcccaatatc acgctatatt
     2221 catatgctga attggaaagc ctcgatggat ttatcggaaa tttcaaagtt aaaatccgta
     2281 agaaagcaaa aagcattgac gagaatttat gtacgggatg tggactttgt actacgaaat
     2341 gtcccgtaaa aaaaatcccc aatgaatttg acgaaaatct aagttacaga aaagccattt
     2401 atgttccttt tccacaagcc gtgcccaaca aacctgtgat tgatagggaa aattgcactt
     2461 attatttgaa agggaaatgc aagatatgcg aaagattttg ccctacacag gctattcgct
     2521 tcgaccaaca agatgaaatt atcgaagttg aggcaggtgc tattgtttta gccacaggat
     2581 ttgatattaa gagagcagat ttcttccccg aatatggctt tggcaaatat aaggacgtta
     2641 tcgatggatt gcaatttgaa cgtattgcct cagcatcggg acctacattg ggcgaaatac
     2701 gcagactatc ggatggaaaa attcctaaga aggtggtttt tattgcatgt gccggttccc
     2761 gtgaccctgc caagggtata gagtactgtt ccaaaatttg ttgcatgtat acggctaaac
     2821 atgccatcct gtataaacac aaaattcacg atggtcaagc gtatgtattt tacatggata
     2881 tccgtgcagg tggaaagaac tatgatgagt ttacccgaag agctatagaa gaagaagggg
     2941 caaaatatat cagaggcagg gtttcgagaa tatatgaacg tgacggaaag ttaatcgttg
     3001 tgggtgctga tacactgatg aatgcccgcc ccgtggtaat cgaagcagat atggtggtat
     3061 tggctacggc aggcgttgct agcccaggtt ctgaagaatt ggcccaaaaa ctccatgttg
     3121 catacgacca gtataagttt
//
LOCUS       DAORPD010000088         3129 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_5488_length_3129_cov_2.972023, whole genome shotgun sequence.
ACCESSION   DAORPD010000088 DAORPD010000000
VERSION     DAORPD010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5488_length_3129_cov_2.972023"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="PK976_01585"
     CDS             <1..741
                     /locus_tag="PK976_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HRC66612.1"
                     /translation="ELSIEQKLTNLYRLQQIESQIDRIRIIRGELPLEVQDLEDEIAG
                     LETRIDNYIQQIAEYEQKILEKKNIIKDCQALIKKYEDQQMNVRNNREYDALSKEIEY
                     QQLEIQLANKRINEFTNELNHLKEEIEKAQKLVEENKSELEIKRSELADIIAETEKEE
                     KELLQKAEKCRKLIEDRLLLAYTRIRKNARNGLAVVHVERDACGGCFNKIPPQHQLDI
                     RMHKKIIVCEYCGRILVDQAIVDSVNQS"
     gene            1136..2362
                     /locus_tag="PK976_01590"
     CDS             1136..2362
                     /locus_tag="PK976_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66613.1"
                     /translation="MESGDQNSPYYRYCLGHFHFQWAIIHLKFKDYYTAFKEANTAYR
                     LLEKNYRLYPQFPPSLSALGVLHVFVGSVPDQYNWIKNLLGYKGTVEQGLSELETALE
                     ESMQNNDLFWLEKETVFYLSFIYINLDKGKEKSLSFRNKYLSQKDIELEKLDPIQAFA
                     FSKVSLFTGRNDEVIRILRSRKFYPDQTEFTFLDYLLANAYQNKLSDSCLIYYDKYLS
                     YPTVKNFKKSSLQRKAWQYLIKEDREEYYLLMKQVLKTGQEEVDADKQAHFAATKGII
                     PNVLLLKSRLLFDGGYYNQALNLLLENKDKLNTLPIIEQTEYHYRLGRIYDETHNLVM
                     AKLEYHKAIELGKALPQYYAANASLMMAMIYEKQHQYPLARHHYKMCMDMPFEEYRNS
                     ITQKAKIGMERTKKMK"
     gene            2414..2875
                     /locus_tag="PK976_01595"
     CDS             2414..2875
                     /locus_tag="PK976_01595"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRC66614.1"
                     /translation="MIRLVDEVFATSSDPNQIRVTPEVMDRLKEMHPSTFAEYSDSNG
                     PVAWIMIIPSTLELMNCFVKGEISENELFELTPSNISYEALYLCSALVLEEYRRQGIA
                     KRLILQAVENIRQQFPIKALFVWPFTKEGELIAENIARKVSLPLYKRLENK"
BASE COUNT         1115 a          497 c          572 g          945 t
ORIGIN      
        1 gaactttcaa tcgaacagaa gttaaccaat ctttaccgat tgcagcaaat agaatctcag
       61 atcgaccgca tcagaattat aagaggtgaa ctgcctcttg aggtacaaga tctggaagat
      121 gaaatcgcag ggctcgaaac tcgcattgat aattatattc aacaaattgc cgagtacgag
      181 caaaaaattc tggaaaagaa aaatattata aaagattgcc aagctcttat caagaaatat
      241 gaagatcaac aaatgaatgt acgtaataac agagaatacg atgcattgtc gaaagaaata
      301 gaatatcaac agctcgaaat acagctggcg aataagcgga tcaatgaatt taccaatgaa
      361 ttgaatcatt taaaggaaga aattgaaaaa gctcagaagc tcgtcgaaga aaataaaagt
      421 gaattggaaa ttaagcgaag tgaattggct gatattatag cagaaaccga aaaggaagaa
      481 aaagaattgc ttcagaaagc tgaaaaatgc aggaaactca ttgaagaccg cttattgctg
      541 gcttatacgc gtataaggaa aaatgccaga aatggcttgg cagtggtgca tgtggaaaga
      601 gatgcttgtg gaggatgttt caataaaatt cctcctcagc atcagttaga tatacgtatg
      661 cataagaaga tcatcgtttg cgaatattgc ggcagaattc tggttgacca agctattgta
      721 gatagcgtaa atcagtcttg ataaaatctt gtattcagtc atgcaccttc tgaatataag
      781 aaggtgtatt tttttaatcg ttgctgataa ttctatcatt aaggacctgt ttatgatacg
      841 gcattattca ttatttttgt tgataataat attctcaatc attctttcta tatcgaaaat
      901 ttctgcacaa aatcaatatt tttttgatat atacaccaca agagcattag aattagcttc
      961 tgaatttcga ttcgaaaaag ccagggagca agtgaatatt tctaaaagcc tgaattctaa
     1021 agcgaatata cccatttggg ttgaaaacta tatcgatttc atttatttac tgaactctga
     1081 agatgataaa caataccaaa atctcaaaaa ttcaaagaaa attcgtctaa aatggttgga
     1141 gtctggagat caaaactcac cttattatag gtattgtctg ggtcattttc attttcaatg
     1201 ggctataatt catctaaaat tcaaagatta ctacacggct tttaaagaag cgaatactgc
     1261 atatcgtttg cttgaaaaga attatcgtct ttatcctcag tttccacctt ctctttctgc
     1321 attaggtgtt ttacatgttt tcgtcggctc ggtacccgat caatacaatt ggataaaaaa
     1381 tctactcgga tacaaaggaa ctgtcgaaca aggtctgagt gaactcgaaa ctgcattgga
     1441 agaatctatg caaaataatg atttattctg gctcgagaaa gaaacagtat tctacttgag
     1501 ttttatttat atcaatcttg ataaaggaaa agaaaaatct ctctcttttc gtaacaaata
     1561 tctaagtcaa aaagatattg agcttgagaa actcgatcct atacaggctt ttgcattttc
     1621 gaaagtgtca ctgtttacgg gtcgaaacga tgaggttatt cgtatccttc gatcgagaaa
     1681 attctatccc gatcaaactg aatttacttt tctcgattat ttactggcta atgcttacca
     1741 gaataaactc tcggatagtt gtttgatata ttatgacaaa tatttaagct atcctacggt
     1801 aaagaatttc aaaaaatcaa gtcttcagcg aaaagcatgg caatatttaa taaaagaaga
     1861 tagagaagaa tattatttat tgatgaagca ggttcttaaa acaggtcagg aagaggtgga
     1921 tgcagataaa caggctcatt ttgcagctac aaagggcatt ataccaaacg ttctattatt
     1981 gaaatcacga cttctttttg atggtggata ttacaatcaa gccttgaatc tgttgttaga
     2041 gaataaagat aaattgaata ctttgccaat tatcgaacag actgaatacc attatcgctt
     2101 aggcagaatt tacgacgaaa cccataattt agtgatggct aaactcgaat atcacaaggc
     2161 aatcgaatta ggtaaggcgc tcccacaata ttatgccgca aacgcatcgc tcatgatggc
     2221 gatgatttat gagaaacaac atcagtatcc actggccagg catcattata agatgtgcat
     2281 ggatatgcct ttcgaagagt atagaaatag tataactcaa aaagccaaga ttgggatgga
     2341 aagaacaaag aaaatgaagt aaattattga atattaaaat gttttaatta tgatttcaaa
     2401 taattatgaa agaatgatca gacttgtaga tgaagttttt gctacttcga gtgatcctaa
     2461 ccaaataaga gttactcccg aagtaatgga tcgtttgaaa gagatgcatc catcaacttt
     2521 tgcagagtat agcgacagca atggtcctgt ggcgtggatt atgattattc cctctacact
     2581 cgaacttatg aattgtttcg ttaaaggaga aatatctgaa aatgaacttt tcgaacttac
     2641 accctcgaat atatcatacg aagcacttta tctttgttct gcgttggttt tagaagaata
     2701 tcgacgtcaa ggaatagcta agcgtcttat tcttcaagct gttgagaata tacgccaaca
     2761 atttcctatc aaggcacttt ttgtgtggcc tttcacaaaa gaaggcgaat tgattgccga
     2821 aaatattgct cgtaaagtct ccttaccact ttacaaacga ttggaaaaca aataattctg
     2881 taacataaaa taatttttaa tcaacacatt gaaaattccg aaggatctaa gccgttaggt
     2941 agtttcgaaa ataaatgtca aattcacaac caagaatttg gctttacact ttattaatcg
     3001 attcgaagac agaagattat ttgaatagca gtggtagcta tatggtgatt atattatata
     3061 atttatggaa ttactgagat ggtccaatga gtctggacaa tatttttatc aaagttaatt
     3121 tattatgtt
//
LOCUS       DAORPD010000089         3129 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_5489_length_3129_cov_2.444697, whole genome shotgun sequence.
ACCESSION   DAORPD010000089 DAORPD010000000
VERSION     DAORPD010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5489_length_3129_cov_2.444697"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="PK976_01600"
     CDS             complement(<1..210)
                     /locus_tag="PK976_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66615.1"
                     /translation="MTIKPGDKVRFLSSSGGGIVTRVIRPGIVAVEIEDGFEIPTAIN
                     DLVKIEETSPAARFFADNDPFLSQKD"
     gene            complement(330..2438)
                     /locus_tag="PK976_01605"
     CDS             complement(330..2438)
                     /locus_tag="PK976_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRC66616.1"
                     /translation="MPVLCLSIVVGFSSCTRTEKITYPETKRVDSVDEYFGQKILDPY
                     RWLENDTSAETSEWVKAQNEITNAYLEKIPFRKSLKERFTQIWDYPKYGIPFSKGNLY
                     FYFKNDGMQNQSVLYVQEGLEGEPRVLLDPNRLSEDGTVAIASFSVSNDARFLAYSIA
                     KAGSDWNEIYVMDINTRQNLPDHITWIKFSGMSWKGDGFYYSRYEAPKPGEELSIKNQ
                     NQRVFYHKLGSDPTKDELIWEDPQHPLRTFGASVTEDERFLIISESESTYGNKLHIKD
                     LQKPASSYIHIGDSFDWEFWVVDNLNDDLIVVTNYLAPRSQAVSINFSHPEISSWQTL
                     IPEKEEVLEGIYLANGKIISQYLKDACNHLYSHNLDGQLIDEIPLPGIGTIGGFSSEK
                     EKNIAFFAFTSFTYPTTIFKFDVEKNQTEVFRKPDIKFNSEDYTTEQVFYTSKDGTKV
                     PMFIVHKKNFLKNGKNPTLLYGYGGFNICLTPSFSISLIPFLENGGVYAEANIRGGGE
                     YGEEWHQAGTKMKKQNVFDDFIAAAEYLISNKYTSPKKLAINGGSNGGLLIGAVLNQR
                     PDLFAVAVPEVGVMDMLRYHKFTIGWAWAYDYGTAEDSEEMFHYLLSYSPLHNIKLGT
                     KYPAVMAMTADHDDRVVPAHSFKYTATLQAAQAGDAPILIRIESKAGHGAGRPTSKII
                     DNVTDKFSFIMYNLGMSPKF"
     gene            2759..>3129
                     /locus_tag="PK976_01610"
     CDS             2759..>3129
                     /locus_tag="PK976_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66617.1"
                     /translation="MTGNYRRETKRIIFKSENFTENDIVGIIMNLEQEYDQNNRLVME
                     RRYDNEGNLEATMSFQYDDSGNLIEQSEIDSSGEFAQKLTFQYDSMSRLIEKDIFYVD
                     ESFDREIYHYNSNNLLDKVEF"
BASE COUNT          968 a          634 c          583 g          944 t
ORIGIN      
        1 gtctttttga gaaagaaagg ggtcattatc ggcaaaaaat cgagctgcag gagaagtttc
       61 ttcaatttta accaaatcgt tgatagctgt tggtatctca aatccgtctt caatttctac
      121 tgcaactata ccgggccgaa tgactcgtgt tactattcct ccgccgctcg agctgagaaa
      181 tcgtacttta tctcctggtt ttatcgtcat agcagggcac atatcatcgt acaaaattac
      241 caattgtaac ttgtttacaa aagaaacccg ggaattaaaa cccgggtttc tcagttatat
      301 aacaatcaat taaatgatgg aattctatct taaaatttgg gactcattcc aaggttatac
      361 ataatgaaag aaaatttatc ggtaacattg tcgataatct tggaggtggg tcttccggcc
      421 ccatgcccgg ctttcgattc aatacggata agtatgggag catcacctgc ttgtgcggcc
      481 tgaagcgtgg cggtatattt aaacgaatga gccggtacaa cccgatcatc atgatcggca
      541 gtcattgcca tgactgctgg atatttcgta ccaagcttga tattatgcaa aggcgaatag
      601 ctcaacagat aatggaacat ttcctcacta tcttcggctg taccataatc ataagcccaa
      661 gcccatccta tagtaaattt atgatagcgc aacatatcca tcacacccac ttcgggtact
      721 gccaccgcaa acaggtctgg cctctgattc aacacagcac caatgaggag gccgccatta
      781 ctacctccat tgatagccag cttcttgggc gatgtgtatt tattcgaaat aagatattcg
      841 gccgcagcaa tgaaatcatc gaaaacattt tgttttttca tcttagtacc cgcctgatgc
      901 cattcttctc cgtattcacc tcctcctcta atgttagctt ctgcataaac tccaccattt
      961 tcgagaaaag gaatcaaact gatactaaat gaaggtgtga ggcatatatt aaacccacca
     1021 tagccatata ggagcgtagg attcttacca tttttcaaaa aattcttttt atgcactata
     1081 aacattggaa cttttgtccc atccttactt gtataaaata cctgttcagt agtataatcc
     1141 tctgaattaa acttaatatc aggtttacga aaaacctcgg tttgattttt ttctacatca
     1201 aatttaaaga tggtagtggg atatgtaaat gaggtgaaag caaaaaaggc aatatttttc
     1261 tctttttcag aactaaaacc tccgatggta ccaatgcctg gaagcggaat ttcgtctatc
     1321 agctgcccat cgagattatg ggaatataaa tgattacaag catccttcag atattgagaa
     1381 ataatcttac catttgccag ataaattcct tcgaggactt cttctttttc ggggataagg
     1441 gtttgccacg aagaaatctc gggatgcgaa aaattgatgg acacggcctg actgcgagga
     1501 gctaaataat tggtgacaac aatcaagtca tcatttaaat tgtcaactac ccaaaattcc
     1561 cagtcgaaag agtcgccgat atgaatgtaa gatgaagcgg gtttttgcaa gtctttaata
     1621 tgtaatttgt tgccataggt tgattcgctt tcagagataa taaggaatct ttcatcttcg
     1681 gtaacgctgg caccaaaggt tcgcagagga tgttgggggt cttcccaaat taattcatcc
     1741 ttggtaggat cacttcctaa cttgtgataa aatactctct gattctgatt ctttatcgaa
     1801 agttcctcac ctggcttagg ggcttcgtag cggctataat aaaatccatc ccctttccac
     1861 gacatacccg aaaattttat ccaagtaata tgatcgggaa ggttctgtcg ggtatttata
     1921 tccatcacat aaatttcgtt ccagtcggaa ccggctttgg ctatggaata agccaggaat
     1981 cgagcgtcgt tcgaaaccga aaatgaagca atggcaacag ttccatcttc ggataaacga
     2041 ttgggatcga gtaaaacccg cggttctcct tcaagacctt cctgaacgta gagtacactt
     2101 tgattttgca ttccatcatt cttgaaatag aaataaagat tccccttgct gaagggaatc
     2161 ccatatttag gataatccca aatttgtgta aatctttcct tcaaggattt ccgaaaagga
     2221 attttttcga gataagcatt ggtaatttca ttttgagcct tcacccattc ggatgtttcg
     2281 gcagaagtgt cattctcgag ccagcggtaa ggatcgagaa ttttttgtcc aaaatactca
     2341 tccaccgaat ctactctttt ggtttcggga taagtgattt tttcggttcg tgtgcaggac
     2401 gaaaaaccta ccactatgct cagacacaac acaggcatga ttttcttgaa ttctatcttc
     2461 atataacaag atatttaaag aatagttaat gaaagtgagg caaagataaa catttggatt
     2521 ctcttgctat ttctgcaaat ggcaaataat aaaaccgtga acaaaaatcc gcagggaaaa
     2581 aatatatgtg caaacaatac ctccttagcc cacacaccca atatccttgc gaatcattcc
     2641 tgttatggct cctactctcg accccatacc tattcatctt ttttatgaag cacctaatat
     2701 gaatcgcttt aagccggaaa tttctatttt tacggccaat taaatcattt aatcagctat
     2761 gacaggaaat tacagaaggg aaacgaagcg tatcattttt aagtcggaaa attttactga
     2821 gaatgatatc gttggcatca tcatgaatct tgagcaagaa tatgaccaaa acaaccgatt
     2881 agtaatggaa agacgctatg ataacgaggg taatttggag gctactatgt cttttcagta
     2941 tgacgactct ggaaatttga tcgaacaatc tgaaattgac tccagtggtg aatttgctca
     3001 aaaactaaca tttcaatatg attctatgtc gagattgata gaaaaagata ttttttatgt
     3061 agatgaatca ttcgacagag aaatttatca ttacaattca aacaatctgc ttgataaagt
     3121 tgaatttat
//
LOCUS       DAORPD010000090         3128 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_5491_length_3128_cov_2.194924, whole genome shotgun sequence.
ACCESSION   DAORPD010000090 DAORPD010000000
VERSION     DAORPD010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5491_length_3128_cov_2.194924"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="PK976_01615"
     CDS             <1..210
                     /locus_tag="PK976_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66618.1"
                     /translation="FYCKRIYGGLNGDLAYWNEKWYRSIGIELTGEFYILRLPMPVSF
                     TIRSSYLPQQKSWRVDVILRADFSF"
     gene            315..866
                     /locus_tag="PK976_01620"
     CDS             315..866
                     /locus_tag="PK976_01620"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRC66619.1"
                     /translation="MKDETLLIKGCLEGNAKMQKCLYDTYASKMFGICLRYANSHAEA
                     SDILQEGFIKVFTKLQDFRNEGSLEGWIKRIMINTAINFCIKEKKHFTTDLDNTGEII
                     EQAAMNYDSLHLKDLMRLVQKLPSGYRTVFNLYEIEGYTHKEISELMGISINTSKTQL
                     MHARRFLQKQLLSIEKQTNSIKR"
     gene            863..1912
                     /locus_tag="PK976_01625"
     CDS             863..1912
                     /locus_tag="PK976_01625"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRC66620.1"
                     /translation="MRQEEEIGKFFKGKFENYAETPPKRVWENIASYLGHPKPVKNIF
                     HQRFFIITITLVGIATLLSIVFLTRKSNQTLQNDFVQNTTTISTSTENKITQQAQTFA
                     FDSSNNKPDRKNEIPASIHSSALLTSKPFPSQESSIVINENRNLPVKAISEAIEAQPE
                     LPIQPEITEPLEIEPAMDFAESLQIQSIETEILNYQVISVCKGEEVFLNAGDGQNYKW
                     NNGAQTQAITFQALENSLFDVNFVNPDGKKVKQTFDIRLLNCSIYIPKAFSPNNDGFN
                     DIYKVEAEGLAEFEMKIFSQWGELVFQSKDIHSGWDGRIHGSRAPSGIYIYQIRYTDP
                     ISHKRAFFGTLTLLP"
     gene            complement(2054..2473)
                     /gene="mscL"
                     /locus_tag="PK976_01630"
     CDS             complement(2054..2473)
                     /gene="mscL"
                     /locus_tag="PK976_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005865683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HRC66621.1"
                     /translation="MMSLLQEFKTFAMRGNVIDMAVGIILGAAFGKIVSSLVNDVIMP
                     PIGLLIGGINFTDIKITLKSAAYNGSNQLIPAVTLNIGSFIQTLVDFIIIAFAIFMMI
                     KAMNQFHKKKEKPASVLAPSNEEKLLTEIRDILKTKK"
     gene            complement(2495..2980)
                     /locus_tag="PK976_01635"
     CDS             complement(2495..2980)
                     /locus_tag="PK976_01635"
                     /inference="COORDINATES: protein motif:HMM:TIGR04366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HRC66622.1"
                     /translation="MSLIAITNPHIEELIRAAQKSKRGRIIFNYHTYAADPVQRMLNA
                     IEPGSYVQPHKHENPDKPEVFIILKGRIAVIIYDDTGEIDDFIILDREEGHFGAEVPP
                     RTWHSIIALESGTVIYEVKHGPYDPTTDKVFAPWAPAENHPQTGEFLLQVTKKLNRFV
                     E"
BASE COUNT         1008 a          572 c          583 g          965 t
ORIGIN      
        1 ttttattgta aaagaatata tggtggcctt aatggcgacc ttgcttattg gaatgaaaaa
       61 tggtatagaa gcataggtat agaattgaca ggagaatttt atattttgag gttgcccatg
      121 cctgtatctt ttactattcg atcgagctat ttacctcaac aaaaatcttg gcgggtggat
      181 gtcattctaa gggccgattt cagcttttga aatttcagga aacaaaaaaa cctccttata
      241 acatattcta aaaaattaat tattcataca accttctttt taatttttat gtctcaatta
      301 aacaggaaac tttcatgaaa gatgaaactc ttctgattaa aggatgtttg gaaggcaatg
      361 caaagatgca gaagtgcctt tatgatactt atgcatcgaa gatgtttggg atatgtttaa
      421 gatatgcaaa cagccacgca gaggcctccg atattctaca agaaggtttt atcaaggttt
      481 ttactaaact acaggatttt cgtaatgaag gttccttaga aggatggatt aaaagaatta
      541 tgatcaatac agctatcaat ttttgtataa aagaaaaaaa acactttacg accgatttgg
      601 acaataccgg tgaaattatc gagcaggctg cgatgaatta tgatagcctg catttgaaag
      661 accttatgag acttgttcag aaattaccct caggataccg taccgttttt aacctttacg
      721 aaatcgaagg ctatacacat aaagaaatat cagaattgat gggtatttct atcaatactt
      781 cgaagactca gctaatgcat gcccgccgct tccttcagaa acaactttta agtatcgaaa
      841 aacaaaccaa ttcgattaaa cgatgagaca agaagaagaa ataggaaagt ttttcaaggg
      901 aaaatttgaa aattatgccg aaactcctcc taaaagggtt tgggagaata tcgcttcgta
      961 tcttggtcat cctaagccag tgaagaatat tttccatcaa cgtttcttta ttataaccat
     1021 tacattagtt ggcattgcta cattgctctc cattgtgttt ctgactcgaa aatcgaacca
     1081 aacattacaa aatgatttcg tacagaatac aacaacgatt agtacttcaa ctgaaaataa
     1141 aattacgcag caagcccaaa catttgcctt tgattcctcg aataataaac ctgatcgaaa
     1201 aaacgaaatt ccagcatcta tacattcaag cgcattactg acaagcaaac cttttccctc
     1261 tcaagaatct tcgattgtaa taaatgaaaa ccgtaatctc ccagtgaagg ctatctccga
     1321 ggctatagaa gcacaacccg aacttcctat acagcctgaa atcacagaac ctttagaaat
     1381 cgaacccgct atggattttg ctgaaagcct tcagatacaa tctatcgaaa ccgaaatcct
     1441 taattaccaa gtgatttccg tatgcaaagg agaggaagta tttctgaatg ccggggacgg
     1501 tcagaattat aaatggaaca atggagcaca aactcaagct attacttttc aggcactcga
     1561 aaatagtctc ttcgacgtaa atttcgtcaa tcccgacggg aaaaaggtaa aacaaacttt
     1621 cgacattcgc ttacttaatt gttctatata cattcctaag gctttttctc ctaataacga
     1681 tggttttaat gacatttaca aggtagaagc cgaaggttta gcagaatttg aaatgaagat
     1741 attctcgcaa tggggtgaat tggtcttcca gtctaaggac attcattctg ggtgggacgg
     1801 ccggattcat ggcagtcgtg ccccttcggg tatatacatt taccaaatac gatatactga
     1861 tcccatttca cataaaaggg cgttcttcgg tacactgaca ttacttcctt aaagcccaaa
     1921 aatacctatt tactgagatc agagaaaggt tgccagcagt gatgctcgca gcctttcaat
     1981 ctattctgca cataggaagt attttgagaa ttttatcgat gaccattttt aatgatatat
     2041 taatttttag gtattatttt tttgttttta gtatatcgcg gatttcggtt agtagttttt
     2101 cttcattaga aggagcaaga acggaggcag gtttttcttt ctttttatga aattggttca
     2161 tcgccttgat catcataaaa atggcaaaag caataataat gaaatcaacc agcgtttgaa
     2221 tgaagctgcc gatattcaat gttacggcag gaattaattg attgcttcca ttgtaagcgg
     2281 ctgacttaag ggtgattttg atatcggtaa aatttatccc tccgattaga agaccaatag
     2341 gtggcataat tacgtcattt acaagtgaag aaacaatttt cccgaaagct gcaccgagga
     2401 taattccaac ggccatgtct attacattgc cacgcatggc aaaggttttg aattcttgta
     2461 acaggctcat aatcgataag ttattattag aaatttattc gacaaaccta ttcaattttt
     2521 tggttacctg caataaaaat tcaccagttt gtggatggtt ctctgcagga gcccagggag
     2581 caaatacctt gtcggtagtt ggatcgtaag gaccgtgttt tacttcatag attaccgtcc
     2641 cagactcgag ggcgataatg gaatgccatg tgcgaggagg aacttcggct ccaaagtgtc
     2701 cttcctcccg gtcaagaatg ataaaatcat cgatttcacc cgtatcatca tagataatta
     2761 cagctatcct cccttttaaa ataatgaaaa cttccggctt atctgggttt tcgtgtttat
     2821 gaggttgtac atacgaacct ggttctatgg cattcaacat ccgttgcaca ggatctgcag
     2881 cataagtgtg ataattgaat attatacgtc cacgtttgct tttttgtgct gctcttataa
     2941 gctcttcgat atgtgggttg gtaattgcta ttaaactcat aatggaatga attttcaaaa
     3001 ttaaaggaag tagatggaat atgcgataaa acaatttaaa gtaaggtatt gcgaaataat
     3061 tttcttatcg aatttttagg atagtctcat tgcagttgcc attacgctca gtttatcgaa
     3121 aagccatt
//
LOCUS       DAORPD010000091         3120 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_5514_length_3120_cov_2.777488, whole genome shotgun sequence.
ACCESSION   DAORPD010000091 DAORPD010000000
VERSION     DAORPD010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5514_length_3120_cov_2.777488"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /locus_tag="PK976_01640"
     CDS             <1..787
                     /locus_tag="PK976_01640"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HRC66623.1"
                     /translation="SVWILTNYTGNPETATWTQINATLANSSSGDNNWVASGDIPLSN
                     FVPTNYTGNIYIGFRYEGTQTNTTTYRIDEVKINTTGGGGGGGGTVTPVSEIHEMFDN
                     VQNDVNIEIEGWLNLAETGTRFWRGKIFNTEKYAQAISYKTLDNQNICWLITPPVMFE
                     GGKLLNFKSAKAYWVHDGLSVWISTDFNGSNLTTATWTPINATLAGKANADHEWVSSG
                     NISISNYVPQGFTGNFYIAFKYIGDAVNTTTYRIDEVNIEPVK"
     gene            complement(1238..1759)
                     /locus_tag="PK976_01645"
     CDS             complement(1238..1759)
                     /locus_tag="PK976_01645"
                     /inference="COORDINATES: protein motif:HMM:NF019468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HRC66624.1"
                     /translation="MNHSKNSSKAEQRPRIKLGWGVTDKFLETTGWVAILLIWIFVSI
                     NYTSMPATIPVHYNANGQADSFGKKSNILLLTAITTVFFVGLTVLNRFPHIFNYPTPI
                     NSQNARRQYTNATRMIRYLKLILVLIFGSIILLTIQYTKGKSEGLGIWFLSLMSVLIY
                     IPLFYFIARSLRK"
     gene            complement(1899..2924)
                     /locus_tag="PK976_01650"
     CDS             complement(1899..2924)
                     /locus_tag="PK976_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66625.1"
                     /translation="MGNAIPTIQDWKNLFDAALKFKKIAPWQWMWDSDIFGVQNPANG
                     EIGYCCIMGAAKEIYALAVYQGSEGLESYLRLNTKYHSESLFDLYTLQKCVMLSFEDR
                     ANLTNEDLQIIKSLGLQFRGHNEWPLFRSFRPGFFPWFIAKEEAEFLTVAIHQAIEVA
                     LQFEKNPNLLSSIDEDYYLVRVPHIENDAIVWKNELLKTQPYKEFEIKTKPVDEKLLE
                     RINKLHILQKDTWEIDYFFYPSAVKEKNERPFYPYIILWVDHRSGYILHHHLANDKQE
                     LAKIPELFLHFIEYFNSIPKTIITKHEMTYQLLEPVAKSLNINLIKKEKLKGIEEAQI
                     AFFENLR"
BASE COUNT          956 a          566 c          599 g          999 t
ORIGIN      
        1 cagtgtatgg atccttacaa attataccgg aaatcccgaa actgccactt ggactcaaat
       61 taatgctaca ttggccaata gctcctcggg cgacaacaac tgggttgcct caggtgatat
      121 tcctctttcc aacttcgttc caacgaatta tacgggtaat atttatattg gattccgtta
      181 tgaagggacc caaaccaata cgactaccta tcgtattgat gaagtaaaaa tcaacacgac
      241 cggaggcggt ggtggaggag gaggtacggt tacccccgtt agcgaaatac acgaaatgtt
      301 cgataatgtt caaaatgacg taaacattga aatcgaaggt tggctaaatc ttgccgaaac
      361 aggcacacgt ttctggagag gaaaaatttt caataccgag aaatatgctc aagccatctc
      421 atacaaaacc ctcgacaacc aaaatatatg ctggttaatc actcctccag tgatgtttga
      481 aggaggcaaa cttctcaact ttaaatctgc caaagcttac tgggtacacg atggcttaag
      541 cgtatggatt tcgaccgatt ttaatggctc taacctcaca accgctacat ggacacccat
      601 caatgcaact ctcgccggca aagccaatgc cgaccatgaa tgggtttctt cgggaaacat
      661 atctatcagt aattatgtcc ctcagggatt taccggaaac ttctatattg ctttcaaata
      721 tattggtgat gctgtaaata caaccactta tcgcatcgat gaagtaaata tcgagccagt
      781 aaaatagagc attctcatta tttttctttt ttttattttt agcggggcaa tccatttcgt
      841 atagctccgg ttttttatat gtgaggcgga atagtgtcgt gagatgtatt tgagtggaaa
      901 gtaaaagaat tacgttgtgc atattatgtg gtctggattg cggagaatgg ataatgtgag
      961 tttaaagtta aaagttcata atctttatga agggaaacag aaaatgggaa aagggagaat
     1021 agggcatagt atcttttgaa cggagtcaac atctgccgaa atgtcatctg aaagtcggtc
     1081 aataaacata ttctttttcc ctctgcgagg agttaatgtg ctcaaaaata atcactcaat
     1141 gattcggagt ttttcttctt tggcaaattt ttccttttca caataatgca attatgattt
     1201 tttttcgagg gtcaagttat tgcattaaag aattgtttca ttttctgagc gatcgagcaa
     1261 taaaatagaa taaaggaatg tatatcaaga ctgacattaa agataaaaac cagattccga
     1321 gtccttcaga cttgcctttt gtatattgta tggtaaggag aataatcgaa ccaaaaatga
     1381 ggacgaggat gagcttcaga taacgtatca ttcttgtagc atttgtatat tgccgcctgg
     1441 cattttggct gtttatcggg gtgggatagt tgaatatatg agggaatcgg tttaagacgg
     1501 tcaaccctac gaaaaaaact gtagtaatag cagtcagaag aagaatattg gattttttac
     1561 caaaactatc ggcctggcca tttgcattat aatgaacagg aatagtggcc ggcatgctgg
     1621 tataattgat cgaaacaaaa atccagataa gcagaatagc cacccatcct gtggtttcga
     1681 gaaatttgtc ggttactccc cacccgagtt ttattcttgg cctttgctca gcttttgagc
     1741 tatttttaga atgattcatt gttattgaat tgtgcaagct cttttcggaa gcatgaccct
     1801 acggatttta tatttgagtt ctgtactcac aaaatccttt cttgctcaaa attaaattag
     1861 tatcctgaat atcactcgaa gaaattgaga attcgatttt atctcaaatt ctcgaagaat
     1921 gcaatttggg cttcttcaat tccttttaat ttttctttct tgataagatt gatatttaaa
     1981 cttttggcaa ctggttcgag caattgatag gtcatttcat gtttcgtaat gattgtctta
     2041 ggtatagaat tgaaatattc tataaaatga aggaataatt caggaatttt agcaagttcc
     2101 tgtttgtcgt tcgccaaatg gtgatgcaag atataaccgg aacgatgatc tacccaaaga
     2161 atgatatagg gatagaaagg tctttcattt ttttctttta ctgccgatgg ataaaagaaa
     2221 taatcgattt cccatgtatc tttttgaagg atgtgtaatt tattgattct ttccagtaat
     2281 ttttcatcta caggtttggt cttaatttcg aattccttgt atggttgcgt tttcagcaat
     2341 tcatttttcc atacgatagc atcgttctcg atgtggggaa ctcttaccag ataataatct
     2401 tcgtcgatag aagaaagcag attaggattt ttttcgaatt gcaaggcaac ctcaatagcc
     2461 tgatggatgg ccacagtcaa aaattctgct tcttccttgg caatgaacca tgggaaaaaa
     2521 ccaggccgaa aacttctgaa caaaggccat tcgttatgtc ctctaaactg taaacctaag
     2581 cttttgataa tttgaaggtc ttcattcgtt agatttgctc tgtcttcgaa actaagcatc
     2641 acgcattttt gaagagtata tagatcgaaa agcgattcgg aatgatattt tgtattcagc
     2701 ctcagataac tctccagacc ctccgaaccc tgatatacgg ccaaagcata aatctcttta
     2761 gctgccccca tgatacagca ataaccaatt tctccatttg caggattttg tactccaaat
     2821 atatccgagt cccacatcca ttgccaaggt gcaatttttt tgaatttcaa agcagcatcg
     2881 aacaaatttt tccagtcttg aatagtaggt attgcgtttc ccattttgaa cctcacattt
     2941 tattgatgtt atttgatttt atatgaacaa tggttggcca agcaataaat ttaattttct
     3001 atatctgttc agacaagcga ttgccttagt atatttattg tgatcatctg tatttttatt
     3061 tcatcactga ccgacaaaaa aattggataa aaagaaggag ggacaaaacc gaagtttcat
//
LOCUS       DAORPD010000092         3102 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_5562_length_3102_cov_2.012143, whole genome shotgun sequence.
ACCESSION   DAORPD010000092 DAORPD010000000
VERSION     DAORPD010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5562_length_3102_cov_2.012143"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..1238
                     /locus_tag="PK976_01655"
     CDS             159..1238
                     /locus_tag="PK976_01655"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HRC66626.1"
                     /translation="MDNQSAVSKYSVFDDTLRFYNALLSDIENATKYIYIEIYRFSYH
                     GLGKKFRDALLKKVHQGVEVKILLDSWGTSPNHDFFSPLTKGGGEVRFFEKIRLNLDY
                     FTRSHRRNHRKIIVIDDEILYLGSANITDYNVVWRELIVRMEGELASVFKDIFLSMFE
                     IYNRYHLYSPELTAPISYGGFNIIRDAPSQRHQNIKKHLIKLIRSARKNIFIETPYFL
                     PSFLLRSALIKAARKGVKVFVILPKHSDVRLVDLLRNKYLGPLTEKGIKFFMYIPQNL
                     HSKLLLVDDNTFAFGSPNFDYRSFSFQHEILLSGHDQRLINLLKDHVNETLQDSEPFD
                     YLRWKRRPYIEKVFEWLIVPFRQLL"
     gene            complement(1338..1877)
                     /locus_tag="PK976_01660"
     CDS             complement(1338..1877)
                     /locus_tag="PK976_01660"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HRC66627.1"
                     /translation="MYRILFNALNGKRLYLIIPMVMMMGMISGCSIGGKIFSSKNEMK
                     KIEKQKETTAEAGVLDSVVKTWLGVPYRYGGTMRSGVDCSGLVREIYKEVYQIELPRS
                     TNDLLPIVQTVGEQNLQPGYLIFYEIEKEKEFHVGIYMGNRKFVHASSTKGVMISSMD
                     EPYFQQRFFKAGKIKNLSY"
     gene            complement(1975..2400)
                     /locus_tag="PK976_01665"
     CDS             complement(1975..2400)
                     /locus_tag="PK976_01665"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRC66628.1"
                     /translation="MMRWKNYPNLFDFFDSLFDREKEEVEGTNYYYTVPAVNIIETDK
                     AFELEVAAPGFSKSDFKITVENNVLRIASDVSKAKEKEEKKMECLRQEFAYGKFARSF
                     SLPNIINTEKISAEYKDGILHVILPKREELTISKQVAIQ"
     gene            complement(2746..>3102)
                     /locus_tag="PK976_01670"
     CDS             complement(2746..>3102)
                     /locus_tag="PK976_01670"
                     /inference="COORDINATES: protein motif:HMM:NF015279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HRC66629.1"
                     /translation="ALHLFPPKTSSWSPKVVLCSSIYQTGISQIWDMINEYINVVKTN
                     GYFWENRKRQSLQSMDDTLHEYLLESFFSNPSIENKYQQLQKKVMNSELSPYKAARQL
                     FDLYLHILSTSKTIGD"
BASE COUNT          926 a          663 c          527 g          986 t
ORIGIN      
        1 aatcgcataa atcgcatctg gtaaatattc ggtacatcaa aaccttccat tccagtgaat
       61 taagtttatt aatgattgac ggcacaaggg ttccggttgc atttcgcagg cgttcacagg
      121 tatcttactc cattgctaac tggtttaagt aacaacttat ggacaatcaa tctgccgtca
      181 gtaaatattc cgttttcgac gatactctga ggttttataa tgctttatta tcggatatag
      241 aaaatgcaac aaaatatatt tatatcgaga tttaccgttt tagttatcat ggtcttggga
      301 aaaagttcag agatgcctta ttgaagaaag ttcatcaggg tgttgaagtt aaaattctat
      361 tagattcgtg gggaaccagc cccaatcacg attttttctc cccactcaca aaaggcggcg
      421 gagaagtaag gttcttcgag aagataaggc tcaatctgga ttattttacg cgtagccatc
      481 gtcgcaatca ccgcaaaatc attgtcatcg acgatgagat tctttatcta ggttcggcca
      541 atatcaccga ttataacgtt gtctggcgcg aactcatcgt aagaatggaa ggcgaacttg
      601 ctagtgtttt caaagacatc ttcctatcca tgttcgagat atacaatcgt tatcatcttt
      661 actcgcccga gcttaccgcg cccatttctt acggaggatt caacatcatt cgcgatgcac
      721 cctcgcaacg ccaccagaac atcaaaaaac acttaataaa attaattcgc tcggctcgca
      781 agaatatatt tatcgaaact ccctactttc tgccttcttt cctcctacgt tcagctttga
      841 taaaggctgc ccgaaaggga gtcaaggttt ttgtcattct ccccaaacat tccgacgtcc
      901 gtttagtcga cttgcttcga aataaatatc tcggcccctt aaccgaaaaa ggaatcaaat
      961 tttttatgta tatccctcaa aatcttcatt caaaactctt actggtcgac gataacacct
     1021 ttgcctttgg ttcaccgaac ttcgattacc gcagcttcag ctttcaacac gaaattttgc
     1081 tgagcggcca cgaccagcgc cttataaatc ttttgaaaga tcatgtcaac gaaaccttac
     1141 aggactcaga acccttcgat tatttgagat ggaaaagacg accatacatc gaaaaggttt
     1201 tcgaatggct cattgttcct ttcagacaat tactctaaat aaaaggaatt gattttgttc
     1261 attattgaat gacctctttc aatatgagaa ttcatcgttg gatctcgagg gaagatggga
     1321 aatactctac ggccttttca ataagaaagg ttttttattt tcccggcttt aaaaaatctc
     1381 tgctgaaaat aaggttcatc catactgctg atcatcactc ctttagtgga tgaagcatgc
     1441 acgaatttgc gattacccat ataaatgcca acatgaaatt ccttttcttt ttctatttca
     1501 taaaaaatca gataaccggg ttgcagattt tgctcaccaa cagtttgaac aatggggagc
     1561 aaatcgttag tagaacgtgg caattcgatc tgataaactt ctttgtatat ttcacgtacc
     1621 aaacccgaac aatccacacc agatctcatt gtacctccat agcgataagg aaccccaagc
     1681 catgttttaa ccacactatc caaaactcca gcttctgcag tcgtttcctt ctgtttctca
     1741 atttttttca tctcgttttt cgacgaaaaa atcttcccac caatcgaaca acccgaaatc
     1801 atccccatca tcataaccat tgggattatt aaatataatc tcttgccatt caaagcattg
     1861 aagagaattc tatacattga taaacagtaa tcttgcatgg caaacattcg tcatgcaata
     1921 ataaaaaaat gggatgtttc agcgaaacat cccactcatt aaggatctcg aaacttactg
     1981 gatagcaacc tgtttgctaa tggtcaattc ttcacgttta ggcagaatca catgcaaaat
     2041 gccatccttg tattcggccg aaatcttttc ggtattgatg atattgggaa gactgaatga
     2101 acgagcaaat ttgccataag caaattcctg acgtaagcat tccatcttct tttcttcttt
     2161 ttctttagcc ttcgaaacgt ccgatgcaat cctcaataca ttgttttcta ccgtaatttt
     2221 gaaatcgctt ttcgagaaac caggagcagc tacttcgagt tcgaaagctt tatcggtttc
     2281 gatgatgttg acggcaggaa cagtgtagta ataattggtg ccttctactt cctctttttc
     2341 acgatcaaaa agactatcga aaaagtcgaa gagattagga taatttttcc aacgcatcat
     2401 agccataatt tttccctccc taatttttaa tgttagacat tgtatttatt tgcgaaaaaa
     2461 aaaggtcaat tcctgtgcca tgagaaaaag taaaataatt tgcgccatta tggcatataa
     2521 atccattatg cttactgcct taatgtcgtg tttataaaaa aatcaaatca atcgattagt
     2581 aatcaatgga tattattttt tgcttcttta cagatgattg ctaaagtcaa aaacgatcag
     2641 catcatttag acaatgatac tttttcaaat tacaaataac aacatttctt ttctccttgt
     2701 ccctccgtct ccttgtctcc cagtctcctc tccttcctcc cttcactaat ctcctattgt
     2761 tttcgaagta ctcaatatat gcaggtagag atcgaaaagt tgccgagctg ccttataggg
     2821 actaagctca ctattcatca cttttttttg taattgttgg tatttgtttt cgatggaagg
     2881 attcgaaaaa aacgactcga gcaagtattc atgaagtgtg tcgtccattg attgaagaga
     2941 ttgacgttta cggttttccc agaaatatcc attagttttg accacattga tgtattcatt
     3001 gatcatgtcc catatttggc ttatgcctgt ttgatagata gaggaacaaa gtacaacttt
     3061 tggtgaccag ctcgatgttt taggtgggaa aagatgcagt gc
//
LOCUS       DAORPD010000093         3080 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_5616_length_3080_cov_2.477355, whole genome shotgun sequence.
ACCESSION   DAORPD010000093 DAORPD010000000
VERSION     DAORPD010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3080
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5616_length_3080_cov_2.477355"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1371)
                     /locus_tag="PK976_01675"
     CDS             complement(<1..1371)
                     /locus_tag="PK976_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HRC66630.1"
                     /translation="MKNRYIDLIEQTFEFPKEEFNVIDNELYWNNIPLMDIIKQYGTP
                     VRLTYLPRISEQIQKARINFNVAMAKTEYNGEYNYCYCTKSSYFSFILEEVLENDVHI
                     ETSSAFDINIIEELYDTGHLKKSNYIICNGFKRPQYIENIVNLIHEGFENTIPVLDNK
                     FELDKYIEAGLKRKTKIGIRIATEEEPQFQFYTSRLGIRYNQIIPYYQEKIQNNPLFE
                     LKMLHFFINTGIKDTAHYWNELLKAVNLYCELKKICPELNALNIGGGFPIKNSLEFNY
                     DYEYMAEEIISQIKNTCMQHGVPEPDIFSEFGTYTVGESGAILFSIVNQKQQNDRELW
                     NMIDGSFMTTLPDTWAISQRYILLAVNNWDVEYQRVHLGGLTCDSQDFYNAEAHSNAV
                     FLPKFNDKEQYIGFFNIGAYQESLSGYGGLQHCLLPAPKHVIIDLDENGEYYTKLFAK
                     EQSYKSM"
     gene            complement(1644..2867)
                     /locus_tag="PK976_01680"
     CDS             complement(1644..2867)
                     /locus_tag="PK976_01680"
                     /inference="COORDINATES: protein motif:HMM:NF013430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HRC66631.1"
                     /translation="METIHNDLSQVNLAETLAPQEQLKPAARTIKRFSIGIPREISPD
                     ENRIALVPSAVQELVQNGHTVYLERGAGNNAKFTDVEFSEAGAKICETTEEVYQAEII
                     VKVAPPKNQEIEFLKPRQVLFSSVQTFIQQKNYFISLLQKKITAFGFEYVKDDSNAFP
                     VMRSMSEIAGTTSVLIAAEYLSHPVFGKGSMLGGFPGINPSEVIILGGGTVAEYAART
                     ALGMGALVKIFDDKIYKLRNIQYQLGSRIFTSIIQPKVLLKSLQTADVVIAALYSREG
                     RTPVIITEEMVNQMKKGSVIVDVSIDQGGCVETSRPTSHSHPIYQVNGVTHYCVPNIA
                     SKVPHTASYAISNVFSPLILKIGYTGGIEKYLTRDPGLRQGVYIFNGILTNKTIGEYF
                     TLPFKNIELLMAAFR"
BASE COUNT          937 a          590 c          558 g          995 t
ORIGIN      
        1 cattgattta tagctttgtt cttttgcgaa taattttgtg tagtattcgc cattctcgtc
       61 gaggtcgatg atcacgtgtt tgggagcagg aaggaggcaa tgttgaagtc caccataacc
      121 ggagagtgat tcttgataag ctcctatgtt gaaaaatcca atgtattgtt ctttatcatt
      181 aaattttggc aaaaagacag cgttcgaatg ggcttcggca ttataaaaat cttggctatc
      241 gcaggtaagc ccgcccagat gaaccctttg atattcaacg tcccaattat tgactgccaa
      301 aagaatgtaa cgctgactga tcgcccaggt atcgggcaaa gtcgtcataa aagaaccatc
      361 tatcatattc cagagttcgc gatcgttttg ttgcttttga ttgacaatag aaaacaatat
      421 ggctccgctt tcccctacgg tataagttcc aaactcgctg aatatatccg gctctggcac
      481 tccatgctgc atacaggtat tctttatctg agaaataatt tcttcagcca tgtattcata
      541 atcgtaatta aactccaacg aatttttaat aggaaaacct ccaccaatat tcaaggcatt
      601 caattcagga caaatctttt ttaattcgca gtacaaattc acggctttca aaagctcatt
      661 ccaataatga gccgtatcct tgattccagt gttgatgaaa aaatgcagca tcttcaattc
      721 aaacaaagga ttgttctgga ttttctcctg ataatacgga ataatttgat tatagcgtat
      781 ccctaagcga gaggtgtaaa actgaaactg cggctcttct tccgtagcaa ttcgaatccc
      841 aatcttcgtt tttcgtttca atcctgcttc gatatactta tcaagctcga acttattatc
      901 cagaacagga atagtattct cgaaaccttc gtggatgaga tttacgatgt tttcgatata
      961 ttgtggtcgt ttgaatccat tgcagattat gtaattcgac ttctttaaat gaccagtatc
     1021 atataattct tcgataatat tgatatcaaa agccgatgat gtttcgatat gaacatcatt
     1081 ttccagaact tcttccagaa taaaggagaa ataagaactc tttgtacaat agcaataatt
     1141 atattcacca ttgtattcag ttttagccat agccacattg aaattgattc tggctttctg
     1201 gatctgttcc gaaattcgtg gaagataagt taatcttaca ggtgttccat actgtttaat
     1261 gatatccatt aatggaatgt tattccagta aagttcatta tctatcacat tgaactcttc
     1321 tttagggaac tcaaaagttt gctcgataag gtcaatatat ctgtttttca tctttaaagt
     1381 aagtgaatta aattagacaa aatgattcaa tttagctaat tcagattttt aaatgaggcg
     1441 caaaaataca acttatatct tcatctttta cgaaaaacga aaatttttta taaaagatat
     1501 cacaatgatc cataatactt taattgaatg attgtggttt gataaaaagt atactggcca
     1561 aattttctta tagaaaactt tcctaaatag caagatgtag ttcacttaca tcttgaaaaa
     1621 tctaagcaag taagtaattt ggattaacga aatgcagcca ttaataattc gatattctta
     1681 aaaggaagtg taaaatattc gccaatggtt ttgttggtta aaatgccatt gaaaatataa
     1741 actccttgtc gtaaacctgg atctcttgtt aaatactttt cgatgccacc tgtgtaacca
     1801 atcttcagaa tgagaggaga aaacacattg cttattgcat aagaagccgt atgaggcact
     1861 ttcgaggcaa tattgggtac gcagtaatgg gttacaccat tgacctgata tatgggatgt
     1921 gaatgcgaag taggccggga agtttccaca caacctccct ggtctatgct aacatcaaca
     1981 ataacagaac cctttttcat ttgatttacc atttcttcgg taatgataac aggagttctc
     2041 ccttcacggc tgtacaaggc agcaatgact acatctgctg tttgaaggct ttttaaaaga
     2101 actttgggtt gaataataga agtaaaaatt ctcgacccca gttgatattg aatatttcgc
     2161 agtttataaa tcttatcatc gaagattttt accagtgcac ccatgcccaa agccgtgcgt
     2221 gcagcatatt cggctacggt gccaccgccc aggataatga cttcggaagg attgatccct
     2281 ggaaaacctc ccaacataga acctttacca aaaaccggat ggctaagata ctccgcagca
     2341 atcagcacag aggtcgtccc agctatttcg ctcatcgaac gcataaccgg aaaggcattg
     2401 gaatcatcct tcacatattc gaacccgaag gcagtaattt ttttctgcaa cagcgaaata
     2461 aaataattct tttgctggat aaaggtttgt acagaagaaa acaatacctg acggggcttg
     2521 agaaattcta tttcctggtt tttgggagga gccaccttga cgatgatctc agcctgataa
     2581 acttcctcgg tggtttcgca aattttagca cctgcttctg aaaattccac atcagtaaat
     2641 tttgcattat tgcctgctcc tctttccaga taaaccgtat gcccattttg tacgagttcc
     2701 tgaacggctg aaggtacaag ggcaattcga ttttcgtcgg gacttatttc acgaggaata
     2761 ccaatgctaa aacgttttat ggttcgagct gctggtttta gttgttcctg gggagccaga
     2821 gtctcggcca aattcacctg gctcaaatcg ttatggatgg tttccatgtt catattatct
     2881 acctgctggt cgttttattc gttatgtggt ggttcaagaa gctgaattca agtcacaaat
     2941 gcaacttttt ggttagacaa atttcgtaat aaaaattcat ttggtgttaa gaaatttaat
     3001 ttttttcttg gtctgttgtt caaaatatct tctactcgct ttatatcctg atcagaaaga
     3061 ttttcaaaag aagtttttta
//
LOCUS       DAORPD010000094         3071 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_5636_length_3071_cov_2.125663, whole genome shotgun sequence.
ACCESSION   DAORPD010000094 DAORPD010000000
VERSION     DAORPD010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5636_length_3071_cov_2.125663"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..547)
                     /locus_tag="PK976_01685"
     CDS             complement(32..547)
                     /locus_tag="PK976_01685"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRC66632.1"
                     /translation="MDIIRSRRSIRRYIPDEVKNEDIEGMLEAAMYAPSAGNQRPWEF
                     IVVRNEAMREIMSKTGPYSFHAAKAPVVILVCGDSLHETHKNYWPVDCSAATENILLE
                     ATARNLGSVWLGVYPRQERMEYLSHLFQLPEHIHPFAMVCIGYANEKPPARGFYDPSK
                     VSWEKFGNKKV"
     gene            951..1517
                     /gene="efp"
                     /locus_tag="PK976_01690"
     CDS             951..1517
                     /gene="efp"
                     /locus_tag="PK976_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HRC66633.1"
                     /translation="MATTADFRNGLCIEFNGEPYTIVEFQHVKPGKGGAFVRTKLKNL
                     RTGRVIPNTFTAGVKIDILRIERREYQFLYADGEDYYFMHNEDFEQISLPKDLIDAPQ
                     FLKEGMICEILFLADNEQPISCELPTHVELEVTYTEPGERGNTATNALKPATVETGAI
                     VKVPLFINTGEIIRIDTRIGDYVERVKD"
     gene            1563..2507
                     /locus_tag="PK976_01695"
     CDS             1563..2507
                     /locus_tag="PK976_01695"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HRC66634.1"
                     /translation="MDFPQLIPLHMLANLLNAKLEGDPQTLVKGLNEIHKAEPGDLTF
                     VDHSKYYQKAFNSQASVILIDQKVECPAGKALLIHPDPFEAYISLVRRFHPFQPSSSM
                     ISPSAIIGEGTIVQPGVFIGNNVVIGKNCIIHANVSIYDHTIIGDRVIIHSGSVLGAD
                     AYYFQNHEGSYRKLESCGKVVVGNDVEIGALCAIDKGVSAETYIGDGTKMDNHVQIGH
                     DTRIGKNCLIGAHSAIAGVTTLEDNVVLWARVSLNKDLVIGKGAVLLATSSVSKSLEG
                     GKTYFGAPAIEARTKWKELAALKQLPDLLQKLHSLLKD"
BASE COUNT          903 a          657 c          545 g          966 t
ORIGIN      
        1 ggaaaataat aacccatcat cttttataaa gttagacctt cttattgcca aacttttccc
       61 acgacacctt tgatggatca taaaatcctc ttgcaggagg tttttcatta gcatagccaa
      121 tacaaaccat agcaaaaggg tgaatatgct ctggaagttg aaataaatgg gataaatatt
      181 ccattctttc ttgtcttgga taaacgccca accaaacact tcccaaattg cgcgccgtgg
      241 cttcgagtag aatattttcg gtagcagccg aacaatccac aggccaatag ttcttgtgcg
      301 tttcgtgaag tgaatcgcca catacaagaa taactacggg agccttagca gcatgaaatg
      361 aatagggccc ggttttcgac attatttcac gcatggcttc atttcttact acaatgaact
      421 cccatggtcg ctggttgcct gccgatggtg catacattgc ggcttctaac atcccttcta
      481 tatcttcatt cttcacttcg tcgggaatat accgtcgaat ggaacggcga cttctaataa
      541 tatccatttt gaattccctt tcttttttgc aaatttattg cattcaatct gctgttgtct
      601 tatcctaagt aatatattgg agcatgtttc tcgctcaatg gctctataat tggtgccatt
      661 caagaattta atcgcctcaa ccaatcgcta ttggaacctc tatttctctt cgaatatcct
      721 ctccttatgc accgaacgtc tatatcgaac tcagattatg cgaatggtaa aaaaattgaa
      781 gctatcacaa ttgacgcatc aacgaatgaa accttataat tttcagcaag agctattttt
      841 attacgtaat atcctattgg ccaatttgtt tgttcaatat aaagattgac ggcaattaaa
      901 aaagctgtac atttgcactc aaattataat ttaattaaaa ccaatgattt atggcaacaa
      961 ctgcagattt caggaatgga ctctgtatcg agtttaatgg agaaccttat acgattgttg
     1021 agtttcagca cgtaaaacct ggcaagggcg gagcttttgt tcgaacaaaa ctgaaaaatt
     1081 tgcgcactgg aagggtgatt cccaatactt ttacggctgg tgtaaaaatc gacattctca
     1141 ggattgaacg acgcgaatat caatttcttt atgccgatgg agaagattat tattttatgc
     1201 ataatgagga tttcgagcag atttcattac caaaagattt aatagatgct cctcaatttt
     1261 tgaaggaagg aatgatatgc gaaattctgt ttttagccga taacgagcag cctatttcgt
     1321 gcgagcttcc tacgcatgtt gagctggagg taacttacac tgaaccggga gaaagaggaa
     1381 atacagctac taacgcctta aaaccggcta ccgtggaaac cggtgctatc gttaaagttc
     1441 ctctttttat caatacgggt gaaattatcc gtattgatac tcggatagga gattatgtgg
     1501 aaagagtaaa agactgattt tgaattcctc ctattttatt accttatacc tcctttaaat
     1561 tcatggattt tccacaactt attccacttc acatgttggc caatttattg aatgctaaat
     1621 tggaaggcga tccccaaact ttagttaagg gattaaatga aatccacaag gcagaacccg
     1681 gcgacctcac ctttgtcgat cactccaagt actatcagaa ggcattcaat agccaggcca
     1741 gtgttattct tatcgatcaa aaagtcgaat gccctgccgg caaggctctg ttgattcatc
     1801 ccgatccttt cgaggcttat atttcactgg taaggcgttt ccatcccttt caaccctcct
     1861 cttctatgat ttcaccttcg gctataattg gtgaaggaac tatcgttcaa cccggagttt
     1921 ttattgggaa taatgttgta atcggaaaaa attgtataat tcatgctaat gtgagcattt
     1981 acgaccatac catcattggc gaccgggtta tcattcactc cggtagcgta ttgggcgctg
     2041 atgcgtatta tttccagaat cacgaaggaa gttaccgcaa attggaatca tgcggaaaag
     2101 tagtcgtggg caatgatgtc gaaataggtg ctttatgtgc catagataaa ggagtatcgg
     2161 ccgaaaccta tattggcgac ggaacaaaaa tggataacca tgtacaaatt gggcatgata
     2221 cacgcattgg gaaaaattgt ctgattggtg cccattcagc cattgctggc gttacaaccc
     2281 tcgaagataa tgtggttctt tgggccagag tatcgctaaa taaagacttg gtcattggga
     2341 aaggagctgt tctattagcc accagcagtg tttcgaaatc actcgaagga ggaaagactt
     2401 attttggtgc gcctgctata gaagctcgca caaaatggaa agaacttgct gccttgaaac
     2461 aattgcccga tttacttcaa aaattacatt ccttgctaaa agattaaaat ttgacatgct
     2521 tttgtcaaat tgaggtatat taattactgt atatactatt ttatttttct cttataaatt
     2581 tgagatgcat cgctatttat tagctatacg tctatagctt gttagagatg atagtaagtg
     2641 atactttctc tctttgatta atgctgattt tctgcgatat ctgcgagggc ttttttctgc
     2701 gatatctgcg agaacatttt tatttttctc tctgatccac cctcttttaa caactaaaaa
     2761 ctacaaaatt ccaaattcca aattacaaac aaactcaaac gaacaactga caacgaacaa
     2821 ctaacaacag acaatcaaca acttaccaaa ttacaaattt cattctctgt cacaccgtct
     2881 cctcgtctcc tcgtccccca atctcacttc ctccctttct ccattctccg ttctcccttc
     2941 ttccgttttt cgttttaaac tttatgaact tttaactttt tacttttaac ttactaatca
     3001 ccatccacta attaccacgt ctcccagtct cctcgtttcc aaatccctaa gtcccattct
     3061 cattccacaa c
//
LOCUS       DAORPD010000095         3066 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_5647_length_3066_cov_2.441049, whole genome shotgun sequence.
ACCESSION   DAORPD010000095 DAORPD010000000
VERSION     DAORPD010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5647_length_3066_cov_2.441049"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="PK976_01700"
     CDS             complement(<1..236)
                     /locus_tag="PK976_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRC66635.1"
                     /translation="MKLISYNVNGLRSALSKGLLEWLEQERPDIFSLQELKAQPDQIP
                     DEEFRRLGYHTYWHCAEKRGYSGVGLISLREPDRV"
     gene            complement(249..851)
                     /locus_tag="PK976_01705"
     CDS             complement(249..851)
                     /locus_tag="PK976_01705"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRC66636.1"
                     /translation="MASTEWNKKYNANEYVYGIEPIPYFIEVLERYRPDNLLLPGEGE
                     GRNAVYAAKKGVKVFAFDQSRTAYQKAQKLAKEHGVQIDYKVCDILESNYPRQYFQMI
                     GIFYLHLPVEVRQKAHQHLLNFLAPGGVLVLEAFHKRNIGNDFGPKTVEMLYDEITLR
                     ADFQSLVIEEYFENHYVFSHGCDDHRGQAELIRFCAIKPH"
     gene            complement(1599..2021)
                     /locus_tag="PK976_01710"
     CDS             complement(1599..2021)
                     /locus_tag="PK976_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008656051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HRC66637.1"
                     /translation="MNLVMIQQQTIVLPEFPKGIHLITDFIMKQLGTLPDRGLLHLFL
                     LHTSAALAINENYDPTVRQDTDYLFDHLVPEHLPAYKHTLEGADDMPAHFKTIIAGSA
                     LTIPITQGRLHLGTWQGIYMCEFRRHGGSRHLVCTIIE"
     gene            2124..2639
                     /locus_tag="PK976_01715"
     CDS             2124..2639
                     /locus_tag="PK976_01715"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HRC66638.1"
                     /translation="MNGTKALTRSRYDKIIGGVAGGLAEYFGMDSAIVRVLFILFTLL
                     YGVGFWVYIVLWIILPYGPLPRRWEQNVPPDFTSNSTPPFSSGGETQINPFPQNQKKL
                     KRDTNMVIGLILIGLGVLFLISQFIPKIHFSHLWPFILIIIGIVLLYNYYQHNKGQSS
                     SENRNKPEQEL"
     gene            2651..>3066
                     /locus_tag="PK976_01720"
     CDS             2651..>3066
                     /locus_tag="PK976_01720"
                     /inference="COORDINATES: protein motif:HMM:NF039187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HRC66639.1"
                     /translation="MEKKFRNVFWGVILLIIGVIFALRNFGIIHFTWRAVFDLWPILL
                     ILWGISILPAKPVLKMILGIFTGAIAITLLLTNPRDESRFYWEGWSYNEHRRYKERNW
                     IPSNQKFVEPYDSAVKTAILKFDAAAGKFTIKDTTN"
BASE COUNT          922 a          600 c          585 g          959 t
ORIGIN      
        1 acccgatcgg gttctctgag tgaaataagt ccaactccac tataacctct tttctcggca
       61 caatgccagt aagtatggta tccgagacga cgaaattctt catcagggat ttgatcgggt
      121 tgagccttca attcctgaag agaaaaaata tcgggacgtt cctgttcgag ccattcgagc
      181 aaccctttcg ataatgccga acgtaatcca tttacattgt acgaaatcag tttcatagtc
      241 cttatgttct aatgaggttt tattgcacag aaacgaatta actctgcttg gcctcgatgg
      301 tcatcgcagc catgcgaaaa tacataatga ttttcgaagt actcttctat tacaagcgat
      361 tgaaaatctg ctcttaatgt gatctcatca tagagcatct caacagtttt gggaccaaaa
      421 tcattcccaa tattccgctt atggaaggct tctaatacca gaactccacc aggagccaga
      481 aagtttaata aatgttgatg tgccttttgc cttacttcaa ccggcaagtg taaataaaat
      541 attccaatca tttgaaaata ttgtcgagga tagttcgatt caagaatatc acaaactttg
      601 taatcaatct gaacgccatg ctctttcgcc aatttctggg ctttctgata agcagttctg
      661 ctctggtcga aagcaaaaac ctttactcct ttcttagcgg cgtaaacggc atttcggcct
      721 tctccctcgc ctggcaataa taaattatca ggcctgtatc tttccaatac ttcgataaaa
      781 tagggaatgg gttcaattcc ataaacatat tcattggcat tatatttctt gttccattct
      841 gtacttgcca taatcaacaa attaagattc tatatacttc cttcgatgaa tatttcgata
      901 cttactcatt ttattcgagg caaaaataca agcctattct gatttttatc ctccccattc
      961 gtcagaaaca attgaatact aaattttttt ggtttgaaac ggaaaacaat aacattatga
     1021 agaaaaccaa tattttggtc attatgtcat gtgttcgggt gcaaccacaa ttcatgccca
     1081 aaagtcaata actggagtaa aaggtagaat cgatttgagc ggcttaaatt ctggacagaa
     1141 gggcgataag agctttaaac ttggagttca tgcgtgaatt tgcggtaaaa ttgttttcaa
     1201 tctcttgtta gcgcatcatc ccgagccttt atattcattg aacggattaa agttagacta
     1261 tgcaatattg ctatctttga gggagaaacc catttttaat ttgcactatg ttgatcttcc
     1321 tctcaaatta gtgttccatc ttacatcgaa tttcgaaata caggtaggga tgtattcagg
     1381 gtgtttactc gcagcaacta cgagcaccaa tgtcagtgta ctcgattatt tccatatttc
     1441 ttttgaaaat gaactcgata gaggggcgtt ctgtagttga gattatggaa taacaggggg
     1501 ttaagccaat atttgactga taaatcatta agaattaaaa ttttctcaaa ttgatatcat
     1561 tgagacaaaa gaaggcatgc ctatgaagag agaccaggct attctataat tgtacaaact
     1621 aaatgacggc tgccaccgtg ccggcgaaat tcacacatat aaattccctg ccatgttcct
     1681 aagtgaagtc ttccttgtgt gataggaatg gtgagagcac ttccagcgat aatagttttg
     1741 aaatgagcgg gcatatcatc ggcaccttcg agcgtatgct tataggccgg caaatgctcg
     1801 ggaacaagat gatcgaataa ataatcagta tcctgtctta ccgtaggatc atagttttca
     1861 ttaatagcca aggctgctga agtgtgaagt aaaaacaaat gaagcaaacc acgatcgggc
     1921 agggttccca gctgtttcat gataaaatcg gtaatcaagt gtatcccttt tggaaattcg
     1981 ggcaatacga tggtttgctg ctggatcatg actaaattca ttaatcaatt aaggtatcat
     2041 cattggcaaa agtaaatatt cagctgtaac ctgttaagga ttgaagcgtc tatttacttc
     2101 gaaaacctta aattatcatt attgtgaacg gaacaaaagc tcttacacga agtcgttacg
     2161 acaagattat tggaggggtg gccggtggac tggccgaata ttttggaatg gacagtgcga
     2221 ttgttcgcgt cctgtttatc cttttcactc tcttgtatgg tgtaggtttc tgggtttata
     2281 tcgttttatg gatcatattg ccttatgggc ccttacctcg acgctgggaa cagaacgttc
     2341 cccctgactt cacttccaat tcaactcctc cattttcttc aggaggagaa actcagatca
     2401 acccttttcc tcaaaatcaa aaaaaattaa aacgcgatac aaatatggtt atcggcctta
     2461 ttttgattgg attgggggta ctatttctca taagtcaatt tatccctaag attcatttta
     2521 gccatctttg gccattcatc cttatcatta ttggcattgt gttgctttat aattactatc
     2581 aacacaataa gggacaatca tcgagcgaaa atcggaataa acctgaacaa gaactttaaa
     2641 tttcaaaaaa atggaaaaaa aattcagaaa tgttttttgg ggagtcattc ttttgatcat
     2701 aggcgtaata tttgctttgc gaaattttgg gattatccat tttacatggc gagctgtgtt
     2761 cgatttatgg cctatattgc tcattttatg gggtatctcg atactgccgg cgaagcccgt
     2821 gctaaagatg atattaggta tattcaccgg agccattgcc attaccctct tattaaccaa
     2881 tcctcgagac gaatctcgct tctattggga aggatggtcg tataatgaac atcgtcgtta
     2941 taaagaaaga aactggattc cttctaacca gaaatttgtt gaaccttacg attctgccgt
     3001 aaaaacggct attctgaagt tcgatgcggc tgccggtaag tttacaatta aagacaccac
     3061 caataa
//
LOCUS       DAORPD010000096         3061 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_5657_length_3061_cov_2.140719, whole genome shotgun sequence.
ACCESSION   DAORPD010000096 DAORPD010000000
VERSION     DAORPD010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5657_length_3061_cov_2.140719"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="PK976_01725"
     CDS             complement(<1..232)
                     /locus_tag="PK976_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66640.1"
                     /translation="MPERLTLLIIIGLAIISCHSPEKGKSNQILEPDLYDTFVAYNQR
                     MTEYENQLIEDYMARYHLSMNQTGTGLRYKILR"
     gene            complement(217..1254)
                     /locus_tag="PK976_01730"
     CDS             complement(217..1254)
                     /locus_tag="PK976_01730"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HRC66641.1"
                     /translation="MIEKNNFYLREAKKIEDLLQTHQNILIISHPYPDGDSIGASLAL
                     YMVFKKLGHEVNIIIPNVHPPFLGWLPAVQNIIIYEQHIQAARKLIQETRLVFCVDFN
                     NLKRLNQLSSLFNEDNHKYVLIDHHPQPDNYFDVKFHTIHVSSTSELVYELLLMMGLE
                     DSIDLDVALNLYVGIMTDTGSFSYSCNYPRTYEITARLIEKGIDAQKTHNLIYDTFSE
                     LRMRLLGYLLLNRMIVMQEYKTAYMWLSLEDQKTFQFQPGDAEGFVNYALAIEGIRLA
                     AFFTEKPDMVRVSFRSKGSFSVNELARKYFDGGGHLNAAGANSYKTLQETLRYFEAVV
                     KENIIALCQKD"
     gene            1362..1784
                     /gene="ndk"
                     /locus_tag="PK976_01735"
     CDS             1362..1784
                     /gene="ndk"
                     /locus_tag="PK976_01735"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: protein motif:HMM:NF001908.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HRC66642.1"
                     /translation="MSGNITFSMIKPCAISRNYTGNIIQFIIEHGFKIIAMKMIWLSR
                     EEAEKFYEIHKERPFYEGLVRFISSAPIIAAILEKENAVDDFRTLIGNTDPAKATQGT
                     IRASYGTDIQQNAVHGSDSDENAQREANFFFSSLERFY"
     gene            complement(1778..2257)
                     /locus_tag="PK976_01740"
     CDS             complement(1778..2257)
                     /locus_tag="PK976_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005643229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRC66643.1"
                     /translation="MIERIRQIMSKKGMTPSQFADAIGIQRSGMSHILSGRNKPSLDF
                     VMKVLNRFPDIDPTWLLKGDTIAKPKQDIKPRELTLFSDEENEFDKDETQNNERPKIT
                     KPSESKPISVKVDEPVAYSPTPKQRSEEVMGKNSPHRISKVILLYEDGFYEIMSNPQ"
     gene            2800..>3061
                     /locus_tag="PK976_01745"
     CDS             2800..>3061
                     /locus_tag="PK976_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66644.1"
                     /translation="MRNTLRLVLIITYSSLFYITANAQQNLSPFEKAVKFADSVYRVN
                     DLRSALSAYRYADKLKPGDTKVQNRIIELEKALSSAEQFNKEA"
BASE COUNT          975 a          545 c          528 g         1013 t
ORIGIN      
        1 cccttagaat cttgtaacga agccctgtgc cagtttgatt catacttaaa tgatatcttg
       61 ccatatagtc ctcgataagc tgattctcat attctgtcat tctctgatta taagctacaa
      121 atgtatcata taaatctggt tcgagaattt ggttgctttt tcccttttcg ggagaatgac
      181 aggatattat agctaaacca ataataatca gcagagttaa tctttctggc ataaagcaat
      241 gatattctcc ttcaccaccg cttcgaaata ccgaagggtt tcttgaagtg ttttgtaact
      301 attggcaccg gctgcgttga gatgcccacc tccatcaaaa tatttccttg ccagttcgtt
      361 taccgaaaag ctgcctttcg agcgaaaact cacacgaacc atatcgggtt tttcggtaaa
      421 aaatgccgcc aatctaatgc cttcgattgc aagtgcataa ttcacaaaac cttcggcgtc
      481 acctggttga aattgaaaag tcttctggtc ctcgaggcta agccacatat aggcggtttt
      541 atactcttgc atcacaatca tgcggttaag cagcaaatac ccgagtaatc gcatgcgaag
      601 ctccgaaaaa gtatcataaa taagattgtg cgtcttttgg gcatctatac ccttttcgat
      661 caaacgggca gtaatttcgt aagtgcgtgg ataattacaa gaataactga aagaacccgt
      721 gtcggtcatt ataccaacgt agagatttaa tgcaacatct aaatcaatgc tatcttccaa
      781 acccatcatg agcaaaagtt catatacaag ttcagaggtt gaactcacat gaatggtatg
      841 aaattttaca tcgaaataat tgtcgggttg aggatgatga tcgatgagaa catacttatg
      901 attatcctca ttgaaaaggc tactaagctg attcagacgt ttgagattat taaaatcaac
      961 acaaaaaacc aaccgggttt cctgaataag tttccgggct gcttgtatgt gctgttcgta
     1021 aattataatg ttttgaactg ctggtagcca gcccaaaaaa ggaggatgaa cgttaggaat
     1081 gataatattt acttcatgtc caagcttttt aaaaaccata tataatgcaa gagaagctcc
     1141 tatcgaatcc ccatcgggat atggatggct aataatgagg atattttgat gagtttgaag
     1201 taaatcctca attttctttg cttctctaag gtaaaagtta tttttttcta tcatgagaac
     1261 gcaaaattat aaaaagcctt tttaatgaac caattcgatc atctgaaaat ataaatgttg
     1321 ctatgtttgc tcattcaaac tactttaaaa tcgattttat catgtcggga aacattactt
     1381 ttagcatgat taagccctgt gctatttcga ggaattatac aggaaacatt attcaattta
     1441 taattgaaca tgggtttaag attatagcaa tgaaaatgat ctggttgagc cgcgaagaag
     1501 ccgaaaaatt ttatgagatt cacaaagaaa gaccttttta cgagggtttg gtaagattta
     1561 tctcatcggc acccattatt gccgccattt tagaaaaaga aaatgccgtt gatgatttta
     1621 gaactttgat tggtaatacc gatcctgcca aggctactca aggaactata agagcatcgt
     1681 atggcacaga tattcagcaa aatgcagttc atggcagcga ttctgacgaa aatgcccagc
     1741 gtgaggcaaa tttcttcttt tcatcgctcg aaaggtttta ttgaggattg ctcattattt
     1801 catagaaacc atcttcgtat agcaaaatga cttttgaaat acgatgaggt gaatttttac
     1861 ccatgacttc ttcgctcctt tgcttgggag tgggagaata agcaacaggt tcatccactt
     1921 ttactgatat aggtttcgac tccgagggtt tggtaatttt gggccgttcg ttattttgcg
     1981 tctcatcctt atcaaattca ttttcttcat cagaaaacaa agtcaattcc ctgggtttta
     2041 tgtcctgttt aggtttagcg atagtatcgc cctttaaaag ccaagttgga tcgatgtcag
     2101 ggaaccggtt caggactttc atcacaaaat cgagacttgg tttattgcgt cccgaaagta
     2161 tatgcgacat ccccgaacgt tgtatcccaa tagcatcggc aaattgtgat ggtgtcattc
     2221 cttttttgct catgatctgt cttattcgct cgatcatttt tcataaaatt tatttacaaa
     2281 tataaactta attttatgat tggatattcc tttatccaaa tgtaactttg taaagttatt
     2341 acaattgtaa cctaatttat aacttgaatt gaattttaat tcacaaatgt aatttgataa
     2401 aataaattat aactatatgg atttaactaa tgttatccat attttaaggc ctttttgtat
     2461 aaattcaaac ttttttaaca tagtcaaaaa ttcgctccat tacttaaagt atcaatttaa
     2521 taaaaaatta tattcatctg gtttataaat aattatatta taatgtaaaa taaattcaat
     2581 tgataattgt aaattgttaa caacaataaa attacagatt gcatttgagt ttaaatatgt
     2641 taattttgta tatttaaatt acaaatgtaa tatcaccttt tatcctattg aaaaaggtta
     2701 aaacaaaata ggtcttcaga atccatcatg aatatactaa ttttgccaga aaaacgtatt
     2761 atatattttg tgttcctgat tgagaatttt tacctgttta tgagaaatac ccttcgcctc
     2821 gttttaataa tcacctattc gtcattattt tatattaccg ccaatgctca gcaaaacctc
     2881 tcccctttcg aaaaagccgt gaaatttgcc gacagtgttt acagagtaaa tgatcttcga
     2941 tcggctttat cggcgtatag gtatgcagat aagttgaaac ctggtgatac aaaagttcaa
     3001 aatcgtatta tcgagctgga aaaggcctta tcatcggccg agcaatttaa taaggaagct
     3061 c
//
LOCUS       DAORPD010000097         3029 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_5738_length_3029_cov_2.219233, whole genome shotgun sequence.
ACCESSION   DAORPD010000097 DAORPD010000000
VERSION     DAORPD010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5738_length_3029_cov_2.219233"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1525)
                     /locus_tag="PK976_01750"
     CDS             complement(<1..1525)
                     /locus_tag="PK976_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HRC66645.1"
                     /translation="MDNTEFKNQILQGIPDELPLPKPYDPNINHAPKRKDILTKEEKK
                     LAVRNALRYFPAKHHAVLAKEFAEELKHYGRIYMYRFRPDYRIYARPIWEYPAQTPQA
                     AAIMLMIQNNLDEAVAQHPHELIIYGGNGAIFQNWAQYLLTMKYLATMTDYQTLVMYS
                     GHPLGLFPSHPEAPRVVVTNGMVVPNYSSPDHWEKFNALGVSQYGQMTAGSYMYIGPQ
                     GIVHGTTITIMNASRKISRKDSASEMKLFVSSGLGGMSGAQPKAGNIAGVVSLVAEIN
                     PKAAYKRFNQGWVDEVIDDVEKAIRTALHWKEMGKPHSIAYLGNVVDLWERLAIDDIH
                     VDIGSDQTSLHNPFAGGYYPVGLSFEESQEMMVSQPEVFKEKVKASLRKQVAAINKIA
                     ANGMYFFDYGNAFLLESGRAGADIFNASGNFIYPSYVQDIMGPLYFDYGFGPFRWVCT
                     SGNPADLDKTDQIAGDIFEKMLTDAPVEIQQQLKDNLLWIRQAKTNNLVVGSQARILY
                     "
     gene            complement(1575..2195)
                     /locus_tag="PK976_01755"
     CDS             complement(1575..2195)
                     /locus_tag="PK976_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRC66646.1"
                     /translation="MEISTEFTEKANRDYQWIQQALHGGDQRAYAKLMDNYRESLYYL
                     MLKMTNDVDDAEDMMMEAFSKAFKNLHQYTPIYAFSTWLFRIAANNCIDFMRKKKNTL
                     SLDASNYTYNCDDTGFDQLSWGLPDDCLDPEQALMKKQSNQFLREIVDQLKPRYKRLV
                     QMRYFQEMSYEEISTELNMPLGTVKAQLFRARALLSGILSQVKHIP"
     gene            complement(2186..>3029)
                     /locus_tag="PK976_01760"
     CDS             complement(2186..>3029)
                     /locus_tag="PK976_01760"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRC66647.1"
                     /translation="RNFQKEYPNLSIVNIEKDLNFFSGKKFPLSIGIKSAKHEIILLT
                     DADCRPASAQWIKTMVSAYTHETSVVLGYSGFYETKGLGNIIQRFNAFHTALQYLSLA
                     MAGMPYMGVGRNLSYKKSLFYQVNGFISHYSLESGDDDLFINQVATKSNTRCSLDSAS
                     FTFSSAKKNFREWIRQKRRHLTTGLHYKPWHKFVLGLYSLSQFLVFFFFIILLLLGFP
                     WPLVVSMFILRLISQLVIYGNAMKKLQTGKLLLISPILEFIEMIVLHYVVLTNRFVKR
                     KQWK"
BASE COUNT          890 a          656 c          612 g          871 t
ORIGIN      
        1 catataagat cctggcctga gaacctacca caagattatt ggtttttgct tgacgtatcc
       61 ataaaagatt gtccttcaac tgttgctgaa tctcgacagg agcatctgtg agcatttttt
      121 cgaaaatatc tccggcaatc tgatcggttt tgtccagatc ggccggattg ccactggtgc
      181 acacccaacg aaacggacca aaaccataat cgaaatacaa tggccccata atatcttgaa
      241 catacgaagg ataaataaaa tttccacttg cattgaagat atcggctcct gccctgcccg
      301 attccaataa gaatgcattg ccataatcga agaaatacat tccattggct gcaattttgt
      361 tgatggctgc cacttgcttg cgcagtgacg cttttacctt ttccttgaaa acttcgggct
      421 ggctaaccat catttcctgt gattcttcga aagaaagacc gaccggataa taaccacctg
      481 caaaggggtt atgcaaagaa gtttgatcgg aaccaatatc tacgtgaata tcatcgatgg
      541 caagcctttc ccataaatcg accacattcc ccagataagc aatcgaatga ggcttaccca
      601 tttctttcca atgcaaagca gttcgaatag ccttttctac atcatcgatc acttcgtcta
      661 cccagccttg gttgaaacgc ttgtaagctg ccttgggatt gatttcggcc accaggctca
      721 ctacgcctgc aatattccct gcttttggtt gcgcacccga catgcctccc aaaccactcg
      781 aaacaaataa tttcatttct gaagccgaat ctttacgaga tattttccgg ctggcattca
      841 taatggtaat cgttgtacca tgcacaattc cctgaggacc aatatacatg tatgaaccgg
      901 cggtcatctg cccatattgg gatactccaa gtgcattgaa tttttcccaa tggtcaggac
      961 tggaatagtt gggcacaacc attccattgg taactactac gcgaggtgct tcaggatgag
     1021 aagggaacaa tcccaaagga tgtcccgaat acatcaccaa agtctgataa tcggtcatag
     1081 tagccagata tttcatggtg aggagatatt gtgcccaatt ctgaaaaata gctccattgc
     1141 ctccgtagat gatcaattcg tgaggatgct gggctacggc ttcatccaaa ttgttctgaa
     1201 tcatcagcat gatggcggca gcctgaggag tttgagcagg atattcccag atagggcgag
     1261 cataaattct ataatcagga cgaaaacggt acatgtaaat tcggccataa tgcttcaatt
     1321 cttcggcaaa ttctttcgcc agaacagcat gatgctttgc tggaaaatat cgtagcgcat
     1381 tacgaacggc taatttcttt tcttccttgg ttaatatatc cttacgcttt ggagcatgat
     1441 ttatgttagg atcgtaaggt ttgggcaaag ggagttcatc cggaataccc tgcagaattt
     1501 gattcttgaa ttcggtgtta tccataatga taatgcaatt gattcgaatc gtaaaaataa
     1561 aaaaattcgt taaattacgg tatatgtttt acttgactca ggatgcccga taaaagtgct
     1621 ctagctctga agagttgagc tttaaccgta cctaagggca tattgagttc agtcgaaatt
     1681 tcttcataac tcatttcctg aaagtaacgc atctgcacca agcgtttata ccttggttta
     1741 agttgatcga ctatctcacg gagaaattga ttggattgtt ttttcatgag agcttgttcg
     1801 gggtcaaggc agtcatcggg taaaccccac gaaagttgat caaaacccgt atcatcacaa
     1861 ttataagtat aattagaagc atccagcgac aaggtattct tcttcttcct catgaaatcg
     1921 atgcaattgt tggcagcaat cctaaaaagc caggtactaa atgcatagat aggagtatac
     1981 tggtggagat ttttaaatgc cttgctaaaa gcttccatca tcatatcttc ggcatcgtcc
     2041 acatcgttgg tcattttcag cataagataa tacaacgatt cgcggtaatt gtccattaat
     2101 tttgcataag cacgctggtc gcccccatgc aaggcttgct gtatccattg ataatctcga
     2161 ttggcctttt cggtgaattc tgtacttatt tccattgctt tcttttaaca aaacgattgg
     2221 tcaataccac gtaatggagt acgatcattt cgatgaattc cagaattggc gaaattaaca
     2281 ataatttacc tgtttgaagt tttttcatgg cattgccata gataactaac tggcttatta
     2341 ggcgtaatat gaacattgaa actactaatg gccatggaaa tccgagcaat aacaaaataa
     2401 tgaaaaagaa aaatacgagg aattgactca gggaatataa gcccagcacg aatttatgcc
     2461 aaggtttata atgcaggcct gtagtcaggt gcctgcgttt ttgtcgaatc cattccctga
     2521 agtttttctt ggctgacgaa aaggtaaacg aagcagagtc gagactgcaa cgagtattgc
     2581 tcttcgtagc tacctgattg atgaataaat catcgtcgcc agactcgaga gaataatgcg
     2641 aaataaaacc gtttacctga taaaacagcg atttcttata gctcaaattg cgacccactc
     2701 ccatataggg catccctgcc atggccagcg aaagatactg caaagccgta tggaaggcat
     2761 tgaaacgctg aatgatattc ccgagtccct tggtttcgta aaagccacta tatcccaaaa
     2821 cgaccgaggt ttcatgggta taagccgata ccatggtctt gatccactgg gcggaagcgg
     2881 gacgacaatc ggcatccgta agcagtataa tttcatgttt tgccgatttt ataccaatgc
     2941 ttaatggaaa tttcttgccc gagaaaaaat tcaggtcttt ttcgatattc acaatcgaaa
     3001 gattagggta ttctttctga aaattcctc
//
LOCUS       DAORPD010000098         3005 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_5807_length_3005_cov_2.441017, whole genome shotgun sequence.
ACCESSION   DAORPD010000098 DAORPD010000000
VERSION     DAORPD010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5807_length_3005_cov_2.441017"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="PK976_01765"
     CDS             complement(<1..254)
                     /locus_tag="PK976_01765"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HRC66648.1"
                     /translation="MELPSIISSFVCGNAIFCRVEVANAPLNVSMGLLAIIILLICSG
                     LISASEAAFFGLSASQIEKIKNSSENYFRRASYLLENPDFL"
     gene            complement(462..878)
                     /gene="ssb"
                     /locus_tag="PK976_01770"
     CDS             complement(462..878)
                     /gene="ssb"
                     /locus_tag="PK976_01770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRC66649.1"
                     /translation="MGNGINKVILVGHLGRDPEIREFNNGKQATFTLATTESYKKSDG
                     QRVDQTEWHNIVLWRGLAEIAEKYLHKGDMIYLEGKIRRREYEDQGQKKYFYTIQGDN
                     FTILSPKKEGIPQVTEAAEPDIEIPPIEGGTDDLPF"
     gene            1017..2765
                     /gene="aspS"
                     /locus_tag="PK976_01775"
     CDS             1017..2765
                     /gene="aspS"
                     /locus_tag="PK976_01775"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HRC66650.1"
                     /translation="MLRTHTCGELNTNHIGEEVILCGWVQRTRDFGGMTFLDLRDRYG
                     ITQLVFNMEWDVHLCEAARKLSREYVIRAKGKVTKRESINPKLPTGEIEIIVDEFEIL
                     NESKIPPFTIEDQTDGGEELRMKYRYLDLRRNPLRKNLEQRHALAMETRKYLCEQHFL
                     EIETPYLIKSTPEGARDFVVPSRMNPGQFYALPQSPQTLKQILMVAGYDRYFQIVRCF
                     RDEDLRADRQPEFTQIDCEMAFVDQEDILQVFEGLIIRLFSKVKGIELDSFPKISYDE
                     AMKRFGSDKPDIRFGMEFTEITSRAKGKGFKVFDEAEIIVGINAKKCAQYSRKELDQL
                     TEWVKRPQIGASGLIYIKFSEDGSLRSSIDKFFRQDHLRELSAEFSAKPGDLMLIIAG
                     AKEKTLKALGELRLEMGNRLGLRNPNVYRPLWVIDFPLLEWDEELQRFFAMHHPFTSP
                     KPEDIPLLETNPSLVRANAYDLVINGVEIGGGSIRIHLKELQNRMLRLLGFTQEEAQK
                     QFGFLMDAFEYGAPPHGGIAFGFDRLCAIFGGSDSIRDFIAFPKNNSGRDMMLDSPSE
                     ISQQQLNELYLRINLP"
BASE COUNT          871 a          594 c          638 g          902 t
ORIGIN      
        1 agaaaatcgg gattctcgag caggtatgaa gctctgcgaa aatagttttc actcgaattt
       61 tttatctttt cgatctggga tgcactcaac ccgaagaaag ctgcttcact cgcggaaata
      121 agacctgaac atattaataa aatgatgata gccaataaac ccatactgac attcaagggc
      181 gcattggcta cctcaactct gcaaaaaatt gcattcccac aaacaaaaga tgaaataata
      241 gaggggagtt ccaagcatga atcaatttat tcccgtgtca aataattgct tacgggctgt
      301 taacttttgc aaagttataa aaaatagaac cctttcttat catttccatt ctcgatttag
      361 ggagtaacgt tccccaaaca agacatgctg aaatgaattg gaaaattttt ctaataagag
      421 aggaaattca ttaacaatga tcgagcagta gagatcgggt tttaaaatgg taagtcgtcg
      481 gtaccgcctt cgataggtgg tatctcgatg tcaggttcgg cagcttcagt aacctgaggt
      541 ataccttcct tcttgggact taggatggta aaattatcac cttgaatcgt gtagaagtat
      601 tttttttgtc cttggtcttc atattcccgg cgtctgattt ttccttccag ataaatcata
      661 tcgcctttgt ggaggtactt ttcagcaatt tcggccagac ctctccatag cactatattg
      721 tgccattcgg tttgatcgac tcgttgacca tcgctttttt tgtaggattc ggttgttgcc
      781 aacgtaaaag tggcttgttt cccattgttg aattcgcgga tttcaggatc acgtcctaag
      841 tgtcctacca aaataacctt gttgataccg tttcccatag tgtttaaatt ttaagatttg
      901 caggtaaatt accattgcca aaggtaagaa aagttttcat tggtgcaacc tttttcaaaa
      961 aaaatttcta atattgattt ttcataattt tgccgcttat tttcaacatt ataataatgt
     1021 taaggacaca tacttgcgga gaattgaata caaatcatat aggcgaggaa gttattttat
     1081 gtggctgggt gcagcgtact cgcgattttg gtggcatgac ttttcttgat ttgcgcgatc
     1141 gttatggaat tacgcaattg gttttcaata tggaatggga tgtacacctg tgcgaggcag
     1201 cacgcaagct tagtcgcgaa tatgtaatca gagctaaagg aaaagtaacc aaacgtgaaa
     1261 gcattaatcc gaaattgcct acgggtgaaa tcgaaattat tgtcgatgaa ttcgaaatac
     1321 tgaacgagtc gaaaattcca ccctttacca tagaggatca aaccgatggg ggcgaagaat
     1381 tgagaatgaa atatcgttat ctcgatctgc gccgcaatcc cttaaggaaa aatctggaac
     1441 agcgtcatgc cttggccatg gaaactcgca aatatttatg tgaacagcat ttcctcgaaa
     1501 tagagactcc ctatcttatt aaatcaacac ccgaaggcgc acgtgatttt gtggtgccat
     1561 cccgcatgaa tccaggccag ttttatgccc tgcctcaatc acctcaaacc ttgaaacaaa
     1621 tattaatggt agctggctac gatcgctatt ttcaaatcgt acgctgcttt agagatgaag
     1681 acttgagagc cgaccgtcaa cctgagttta cacagataga ctgcgaaatg gcttttgtgg
     1741 atcaggaaga tattttacaa gtctttgaag gtcttattat tagattgttt tcaaaagtaa
     1801 agggaataga attggattct tttcccaaaa tttcatacga cgaagccatg aagcgctttg
     1861 gttccgataa acccgatatt cgcttcggaa tggagttcac cgaaattact tctcgggcaa
     1921 aaggaaaagg attcaaggtt tttgatgaag ccgaaataat cgtaggaatc aatgccaaga
     1981 aatgtgctca atatagccga aaggaactcg accagctgac tgagtgggta aaaaggccac
     2041 aaattggcgc ctcggggctt atttatatta aattttcgga agatggtagc cttcggtctt
     2101 cgatagataa attttttcga caagaccacc tgagagaatt atcggcagag ttctcggcaa
     2161 aacctggcga cttaatgttg ataatagcag gtgcaaaaga aaaaacgctg aaggctttgg
     2221 gtgaactcag gctcgaaatg ggcaaccgtc ttggattgcg aaatcccaat gtatatcgcc
     2281 ctctctgggt tatcgatttt ccactcctgg aatgggatga agaattacaa cggttttttg
     2341 ccatgcatca tccctttact tctcctaaac ccgaagatat tcctttactt gagacaaatc
     2401 cttctttagt gagagccaat gcttatgatc tggttatcaa tggagtagag attggaggag
     2461 gtagcatacg tattcatctc aaggagctgc aaaatagaat gcttcggttg cttggtttta
     2521 cccaggaaga agcccagaaa cagtttggat ttctgatgga tgccttcgaa tatggagctc
     2581 cacctcatgg aggtatcgct tttgggttcg accgtctctg cgctatcttt ggaggttccg
     2641 attccatcag ggattttatt gcttttccta aaaataattc cggccgtgat atgatgctgg
     2701 attccccttc cgagatttca caacagcaac tcaatgaact ttatctaagg attaatctac
     2761 cataaaaacc ttacggggat tcaaaaacct gttagggtta aaatttgaag ctgtgtcaaa
     2821 ttcgaacctt tccttcagaa ttgtaggatg ccaatgattt ctcttacttc gcgaatggtt
     2881 ttttgcgcgc tggcacgtgc ctttgctgct ccttcggtta ctaccttgtg cagatagtca
     2941 ttatcggcat cgagttgcag aattttttca cggaaaggcg tcgtaaactg gataatatct
     3001 tcggc
//
LOCUS       DAORPD010000099         2997 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_5829_length_2997_cov_2.186948, whole genome shotgun sequence.
ACCESSION   DAORPD010000099 DAORPD010000000
VERSION     DAORPD010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5829_length_2997_cov_2.186948"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="PK976_01780"
     CDS             complement(<1..359)
                     /locus_tag="PK976_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRC66651.1"
                     /translation="MKAEIGKMIIGQHETIEKVITCIFSQGHCLLIGVPGLAKTLLVN
                     TVAKVLGLSFNRIQFTPDLLPADVIGTMIFSQKQEAFNIRKGPVFANFVLADEINRAP
                     AKVQSALLEAMQERQVT"
     gene            complement(445..1791)
                     /locus_tag="PK976_01785"
     CDS             complement(445..1791)
                     /locus_tag="PK976_01785"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRC66652.1"
                     /translation="MDLFLFVTSVGLFAQESAVIDEIVAVVGNKVILLSDIENQYIQY
                     RAQGNIRGGAEQTKCAILESLLFNKLLLNQADIDSVEVTDKQVESELDRRIKYFIQQI
                     GSKEKLEEYFKKSLNEIKNEFREIIRDQMRMEMVQYNLTKNVKITPTEVRSYFKKMPS
                     DSIPMVNSEYVIGQIVKKPKTSLADEIEVKEKLRALRKRILEGENFATLAVLYSEDPA
                     TAARGGEVGLYGRGELYPEYEAVAFKLKEGEISDIVQTKAGYHIIQLIERRGDYVNTR
                     HILMTPKISPEELQKAATFLDSVAQLINNKKITFEEAVDKFSDDPNKMNGGLMINPVT
                     GTTRFEPDHLDPKVFYVIDKLEPGQVSGPVPFKTDEGKDAYRLLYLKEKSKPHRANLK
                     DDYDRIQNWALEYKKDQVLQKWVKERIRQTYIFINPAYANCQFQHQWTDRTGEDYK"
     gene            complement(1830..2672)
                     /locus_tag="PK976_01790"
     CDS             complement(1830..2672)
                     /locus_tag="PK976_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66653.1"
                     /translation="MFSCLMILCFAGCNYIKKKFRPDETILARVHDDYLYSSDIKGIV
                     PEGTPREDSILIVKNFIENWVRNRVELHHAEKNLSADEKNFKEQLDDYRNSLIIYTYE
                     SHLIRQKLDTMVSIEEIEDYYRKNKQNFVLREPIVKMIYVKISKDSLRAAQLARKFLQ
                     PDTILDYDAFERFCFTRTSDYYISINNWFLFRDLQNMAPITTFNEELFLKNNRLIDLT
                     DDQWRYLVRIYAYVTKDGISPLSMEMENIKNIILNTRKQELIRNMRENLYEKALKSDD
                     IEFY"
BASE COUNT          821 a          626 c          565 g          985 t
ORIGIN      
        1 atagtaacct gacgttcctg catggcttca agcaatgcac tttgaacttt ggccggagca
       61 cggttaattt catcagccag tacaaaattg gcaaataccg gtccttttcg gatattaaac
      121 gcttcctgct tctgactgaa aatcatggta ccaatgacat cggcaggtaa taaatcgggt
      181 gtaaattgaa tacgattgaa cgaaagcccg agaactttag ccacggtatt aaccaacagc
      241 gtcttggcta gcccaggtac accaatcagt aaacaatgcc cttggctgaa gatgcaagta
      301 attacctttt cgatggtttc gtgctggcca atgatcattt tgccaatctc ggctttcatt
      361 ttttcataaa catcgtgaag ctgctgtatg gattccgttt cttcgtaagt tttcatgaaa
      421 aagaattaac tttattagat gttgctattt gtagtcttcg ccagttctgt cggtccattg
      481 atgttggaac tggcaattgg cataggcagg attaataaag atataggttt gcctgatgcg
      541 ttctttcacc catttttgca gaacctggtc ctttttatac tctaaagccc aattttggat
      601 gcgatcgtaa tcgtctttta gattagcgcg gtggggtttg cttttttcct taagatagag
      661 caagcgataa gcgtctttcc cctcgtcggt tttaaatggg acagggcctg aaacctggcc
      721 gggttccagt ttgtcgatta cgtaaaatac tttgggatcg agatgatcgg gttcgaaacg
      781 tgtagttccc gtaacaggat tgatcatcaa accaccattc attttattgg gatcatccga
      841 gaatttatcc acagcttctt cgaaagtgat tttcttatta ttgataagct gagccacact
      901 gtcgagaaat gtggctgctt tttgaagttc ttcgggtgag attttcgggg tcattaggat
      961 gtgtcgtgta ttcacataat cgccacggcg ttcaatgagt tgaataatat gataacctgc
     1021 tttggtttga acaatatcgg aaatctcacc ttctttcaac ttaaaggcaa cggcttcgta
     1081 ttcgggatat aattctcctc tgccataaag tcctacttcg ccacccctgg ctgccgtagc
     1141 cgggtcttca ctgtaaagaa ccgccagggt agcaaaattt tcaccttcga gaattcgctt
     1201 gcgcaaagcg cgcagttttt cctttacctc gatttcgtcg gctaaactcg ttttgggctt
     1261 cttcacaatt tgtccaataa catactcgga attcaccatg ggaatactgt cggagggcat
     1321 cttcttgaaa tatgaacgaa cttcggtagg tgtaatcttc acatttttcg tcaaattata
     1381 ctgaaccatt tccatgcgca tctgatcacg gatgatttcc ctgaattcat tctttatttc
     1441 attcaacgac ttcttgaaat attcttctaa cttttctttc gaaccaattt gctgaataaa
     1501 atattttatt cgtctgtcga gttccgattc gacttgttta tcggtaacct ccacgctgtc
     1561 gatatcagcc tgattgagca gcagtttatt aaacagaagg ctttcgagaa tggcgcattt
     1621 ggtttgttcg gctccccctc tgatatttcc ctgcgctctg tattgtatgt attgattctc
     1681 gatgtcggaa agtaaaatca ctttgtttcc tactaccgct actatttcat ctatcacagc
     1741 actttcctgg gcaaaaagcc caacgcttgt tacgaacaaa aacaaatcca aaaaaaatat
     1801 tctaaatttt ttcattattt atcaaacagt taataaaatt cgatatcatc gctcttcaaa
     1861 gctttttcgt aaaggttttc gcgcatattc ctgattaatt cctgtttacg agtattgagt
     1921 atgatatttt tgatgttttc catctccatg ctcagaggtg aaataccatc tttggttaca
     1981 taggcatata tgcgtaccag gtatctccat tggtcatcgg tcaggtcgat caatcgattg
     2041 ttcttcaaga aaagttcttc attgaaagtg gtaatggggg ccatattctg caaatcacga
     2101 aacaaaaacc agttattgat actgatataa taatcactgg tacgggtgaa gcaaaacctt
     2161 tcgaatgcgt cgtaatcgag tattgtatct ggctgcagaa attttctggc caattgcgca
     2221 gctctcaacg aatctttcga aatcttcaca taaatcattt taacaatggg ctctcgtaaa
     2281 acgaaatttt gtttgttctt tcgataataa tcttcgattt cttcgataga aaccattgta
     2341 tcgagttttt gccgaattag atggctttcg taagtatata taatcaaaga attacgataa
     2401 tcgtccaact gctctttgaa atttttctcg tcagcggaaa ggttcttttc ggcatgatgt
     2461 agctcgacac gattacgtac ccagttttcg ataaaatttt tcacaataag tatactgtct
     2521 tcgcgagggg ttccttcggg cacaattcct tttatgtcag aactatacag ataatcatca
     2581 tgaactctgg ccagaatggt ttcatcgggc cggaatttct ttttgatgta attgcatccc
     2641 gcaaaacaca atatcattaa acacgaaaac aaaaaaatct tacatctcat tcgaatcaat
     2701 ctatcacttt taaacatcat ccacattgta tcataaaaaa tcaggtcttt tattcgacat
     2761 aacatctcaa gcatataacc tcgccatgct ctatatattg cgaatccctt cgggaatggt
     2821 ttcgagtcat ttgccttatc ttctcatgca tgatataatt ataagaatcg ggttaattcg
     2881 gcatcgtgat catcgtgaga aaatgccaag gggttgccct gatgaatgaa gagaaaaatc
     2941 ctgttcatca aaagctttca atatcagttt cgagcacaga tcgtctcgaa tatcttg
//
LOCUS       DAORPD010000100         2980 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_5868_length_2980_cov_2.360342, whole genome shotgun sequence.
ACCESSION   DAORPD010000100 DAORPD010000000
VERSION     DAORPD010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5868_length_2980_cov_2.360342"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="PK976_01795"
     CDS             complement(<1..301)
                     /locus_tag="PK976_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007214719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase subunit F"
                     /protein_id="HRC66654.1"
                     /translation="MRNDNIYKPCLMRIEKVTDEAPDVKTFRLKFIDDVQARNFDFKA
                     GQFGEYSVMGEGESTFCIASPPTRKGYIECTFRKTGRVTSALANKEEGEIIGFRGP"
     gene            complement(301..1317)
                     /locus_tag="PK976_01800"
     CDS             complement(301..1317)
                     /locus_tag="PK976_01800"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRC66655.1"
                     /translation="MTPQNKLLKKECLPTFVETLVKGGKLLYAPTKENERIEFKLIEN
                     FSEIYLDYIQTTKSIKSIVFPPIEKLFEYVADKEHVEIKDVNLNTIPETIVFGAHPCD
                     AASFKVLNAVFTWDCNDEFFLTRKDKTTVIGLSCKNHDDYCFCTSVDLNPGSTEGSDI
                     LLTRIENDNYLAEIMTEKGEAVYKLVKNLFEPAPQVDKNAFVTQLPKLFDLNDLRGKL
                     SFEGLSWMQQSLRCLSCGTCAYVCPTCSCFDIQDIGNQTKGKRVRIWDTCALPQFTLH
                     TSGHNPRHFQNERWRQRIMHKFSYEPERLHVYGCVGCGRCSRACPVNINILENLIEIS
                     GGNK"
     gene            complement(1322..2122)
                     /locus_tag="PK976_01805"
     CDS             complement(1322..2122)
                     /locus_tag="PK976_01805"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRC66656.1"
                     /translation="MDRVKQKARDLLDSNKVQVVIGYEEGTDGTARPVFIMDSKNIDK
                     LIFNDRCYHNLAVYLTKVEVEKIGRVAVEASLNTLKGIVKLASEGQFVFEDIDILGVN
                     RNGELYEFASLDEVIDYIDQHPVEYSPEEISLLRKIESMTREERWEYWQEEFSRCIRC
                     YACRAACPNCYCDACIVDINQPQWISPTPDALGNLEWHIIRAMHLAGRCVNCGACAKA
                     CPVDIPLNILNLKLAEEISKNFGISEEFLLTNYPLAIYDPNDKENFIK"
     gene            complement(2115..2561)
                     /locus_tag="PK976_01810"
     CDS             complement(2115..2561)
                     /locus_tag="PK976_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018462502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HRC66657.1"
                     /translation="MDEFEPKIVAFVCNWCTYAGADLTGTSRIKYAPNVRIIRFPCTG
                     RIDFMFILKAFARGADGIIVSGCHPNDCHYTSGNFHARRRWIVFRSLLDFLGIDVRRI
                     TFSWVSAAEGAKWADLVNQTTAEIRKLGPYTSYHKISKPEEEVIYG"
     gene            complement(2563..>2980)
                     /locus_tag="PK976_01815"
     CDS             complement(2563..>2980)
                     /locus_tag="PK976_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007217450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRC66658.1"
                     /translation="VETNTAGIFLAGACQSIKDIPESVSQASGAAAKVCGLFSQNELT
                     RDPLIAVVNRTAPPLYSTCVGCFLCQSVCPYQAIEKEEIKAKDGSVIKIIAKINPGLC
                     QGCGSCVALCRTKSIDLLGYTNRQIYAEVDAILNTL"
BASE COUNT          847 a          644 c          539 g          950 t
ORIGIN      
        1 aaggccctct gaagccaatt atttctcctt cttccttgtt tgccaatgcc gatgtaaccc
       61 ttcctgtttt tctgaacgta cattctatat aaccttttcg cgtgggtgga gaagcaatac
      121 aaaaagtcga ttcgccttcg cccatgacag agtattcgcc aaattgaccg gctttgaaat
      181 caaaatttct ggcttgtaca tcgtcgataa atttcagtcg aaacgtcttt acatcgggtg
      241 cctcatcggt aaccttttca attctcatca aacaaggttt ataaatattg tcgttgcgca
      301 ttatttgttt cctccactaa tttcaatgag gttttccaaa atattgatgt ttaccggaca
      361 ggctcttgaa caacggccac aaccaacgca gccgtaaaca tgtaaccgtt ctggctcata
      421 agaaaattta tgcataatac gttgcctcca acgttcgttt tgaaaatggc gtggattatg
      481 ccccgacgta tgcagggtaa attgaggcaa agcacaggta tcccatattc taaccctttt
      541 gcctttggtt tgattgccta tatcttgaat atcgaaacaa gagcaggtag ggcacacata
      601 agcacatgtt ccacagctaa gacaacgtaa tgactgttgc atccatgaaa gaccttcgaa
      661 cgacaatttt cctcgtagat cattcaaatc gaaaagtttg ggcaattggg ttacaaaggc
      721 atttttgtcc acttggggag caggttcgaa caaatttttt acaagtttat atactgcttc
      781 acctttttca gtcataattt cggccaggta attatcgttt tcgattctcg tcagcaaaat
      841 atcgctgcct tcggtcgaac caggattcaa atccaccgat gtacaaaaac aataatcgtc
      901 atgattttta caactcagcc ctataactgt agttttatcc ttgcgggtaa ggaaaaattc
      961 atcattacaa tcccatgtaa aaacggcatt cagtaccttg aatgacgcag catcacaagg
     1021 gtgtgcgcca aatacgatgg tttcggggat agtattcaaa ttcacgtcct tgatttcgac
     1081 atgttcctta tcggctacgt attcgaataa tttctctatt ggcgggaata caatactttt
     1141 aatagattta gtggtttgga tataatcgag atatatctcg ctgaaatttt ctatgagttt
     1201 aaattcaatc ctttcatttt cttttgtagg ggcatacaac aattttccac ctttgaccaa
     1261 ggtttcaaca aacgtgggga ggcattcttt tttcaataat ttattttgtg gtgtcatagc
     1321 cttatttaat aaaattttct ttatcgtttg gatcataaat agctaaagga taatttgtta
     1381 gcaaaaattc ctcagatatt ccaaagttct tactaatttc ctctgctaat ttgaggttta
     1441 agatgtttaa tgggatatca acggggcatg ctttggcaca ggcaccacaa ttgacacaac
     1501 gaccggctaa gtgcattgct ctgatgatat gccattctaa atttcccaag gcgtcgggag
     1561 taggagaaat ccattgtggc tggttaatat ccacgatgca agcatcgcaa taacaatttg
     1621 ggcatgctgc acgacatgca taacatcgaa tgcaacgcga aaactcttct tgccagtatt
     1681 cccaacgttc ttccctagtc atagattcga tcttccgaag aagggatatt tcttcgggac
     1741 tgtattcaac aggatgctga tcgatatagt ctatcacctc atccaaactg gcaaattcgt
     1801 ataattcgcc atttcggttt acacccaaaa tatctatgtc ttcgaacaca aattgacctt
     1861 cagatgctaa ttttacaata cctttaagtg tattcaatga agcctccaca gccactcttc
     1921 caatcttctc gacttcaacc ttggttaaat aaacagcaag attatgatag caacggtcgt
     1981 tgaaaatgag tttatctata ttttttgaat ccatgataaa aaccggtctt gccgttccgt
     2041 ctgtaccttc ttcatagcca atcaccactt gaaccttatt cgaatcaagc agatctcttg
     2101 ctttttgttt tactctatcc atagattact tcctcttctg gtttagatat tttatggtaa
     2161 ctggtatagg gtcctaattt acggatttcg gcagtagttt gatttaccag gtcggcccat
     2221 tttgcacctt cggcggccga aacccatgaa aaagttattc ttctaacgtc gatacctaaa
     2281 aaatcgagta aacttctgaa tactatccat cttcttcgag catggaaatt ccctgatgta
     2341 taatgacaat cgtttggatg acatccagaa acaataattc catcggctcc tcgggcaaat
     2401 gctttgagaa tgaacataaa atcaattctt cctgtgcatg ggaatcgtat aatccttacg
     2461 ttcggagcat attttattct gcttgttcct gtgaggtctg cgcctgcata agtacaccaa
     2521 ttacatacaa aggctacgat ttttggttcg aattcgtcca tgttataatg tatttaaaat
     2581 tgcatcaact tctgcataaa tttgtctatt ggtatatccc aagaggtcaa tagacttggt
     2641 acggcaaagt gccacacatg aaccacaccc ctgacacaaa ccgggattaa tttttgctat
     2701 aattttaatt acgcttccgt ccttagcctt gatttcttct ttttctatag cctgataggg
     2761 gcatacactc tgacataaga aacagccgac gcaggtagaa tacaagggag gtgccgttct
     2821 attaaccacg gcaatcaatg gatctcgagt aagctcattt tgcgagaata acccacaaac
     2881 tttggcagcg gctcctgatg cttgtgatac tgattcagga atatccttaa tgctttgaca
     2941 ggcaccggct aaaaatattc ctgctgtatt ggtttctacc
//
LOCUS       DAORPD010000101         2978 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_5874_length_2978_cov_2.804995, whole genome shotgun sequence.
ACCESSION   DAORPD010000101 DAORPD010000000
VERSION     DAORPD010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5874_length_2978_cov_2.804995"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            160..>2978
                     /locus_tag="PK976_01820"
     CDS             160..>2978
                     /locus_tag="PK976_01820"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HRC66659.1"
                     /translation="MNDKEILIQLYYQKIKASSKELTKKEAFKDLLNRLYSSDKEIRS
                     IIDRITLGAETTVVNIPKKDKLHRGSADTLYNNIIIEFENDLKVSYQHAKEQLAGYLL
                     GQFRSGKGYNFTLIVSDFINWKVLAPDISCLDKLDDLHENELILNEIESASFVLDESN
                     AEEFYYWIDRFLFKEEKQKATLKSIEESFGYQSSVFIESFRELNNWFNEAKKYGIVQV
                     SFEQWYKFLSIAYGSFDGSESIFLIHTYLSIFAKMLAYAVVSNDDYISDDEMREILNG
                     SIFQSYNISNFVDNDFFHWVNGDRDYLKLKNVFRLIAQEISTFNFSNVDEDILKGVYQ
                     ELIDIDTRHSLGEYYTPDWLCERIVNEYEFKKTDRILDPACGSGSFLRAFIHRIRELN
                     PDATAEEINNQIYGIDIHPLSVQIAKTTLLLALGKGISRARRPIHLNIILANTLLAPE
                     GVQNAFGNDFVMQIDKDKYIINTQIFDNVEVFDAFLDICEELSEQTLNKKDIELSLFE
                     DILKHHTNGGLCSQLVESFYKIYQGVKRVKEKGRNGIWKFILSNLYKPFFLANRFDYV
                     IGNPPWFTYSSIKNKEYQDLLDELAVKYNVKPQKVANYPHLEIAAIFQSYCSSYFLND
                     WGKQAFVLPRSFFSADHHENSRSGIAKGFRLSSIWDLDNVSPLFRIPCCVFFSEKKQK
                     ERIMMSSGLDGKIFVGKLPTHNCNYAIAKNILVETPVKWYYIRQGQSSALSTRKIEMQ
                     RNVNPYKNLFKQGATIVPRAFYFVELDQQMPDDFNDRIISLRTSKFVKADSKDPWKLE
                     FSGRMESRFLFRTAISKSILPFHLYKPDFVVLPLIIQINENQFKKIEIHTADDIMNMG
                     YLNASRWFRNAERIWNINKTEKSKNMSTIGRLNFHNGLTQQNLNAEYLVLYNSSAKDA
                     NALVVNRSSLDLEFICESKSYVYYL"
BASE COUNT          962 a          453 c          585 g          978 t
ORIGIN      
        1 aaacgataaa tggtataccg ctgaaattac caagaaaact aatgaccttt tgaagaaatt
       61 agaactacct attacgtaaa tttcgcgaag ttagggttta atcgtaaatt ttcttatcga
      121 gattaattat ctacttttga tgaatcgaat tcttgatata tgaatgataa agaaatcctt
      181 attcagctat attatcagaa aattaaagca tccagtaagg agctgacaaa aaaagaagcc
      241 tttaaagatt tattgaacag actttattct tcggataagg aaattcggag tataatagat
      301 agaattactc ttggagcgga aaccacagtt gttaatattc ccaaaaagga taaattgcac
      361 cgtggaagtg ccgacaccct ttataataat attatcatcg aatttgagaa cgatttgaag
      421 gtttcttatc agcatgctaa ggaacagttg gctggttatt tattgggtca attcagatca
      481 ggtaaaggct ataatttcac actcatagtt tcggatttta tcaattggaa agttcttgca
      541 cctgatatca gttgtttaga taagctggac gatttgcatg aaaatgaatt aatcttgaat
      601 gaaatagaga gtgcttcttt tgtgctcgat gagtcgaatg ccgaagaatt ttactattgg
      661 atagacaggt ttttattcaa agaagagaaa caaaaagcaa cgcttaaaag catcgaagaa
      721 tcttttggat atcaaagttc cgtttttatt gaatcattta gagaattgaa caactggttt
      781 aatgaagcga aaaaatacgg aatcgtccag gtttcttttg aacaatggta taaattttta
      841 agcattgcgt atggttcatt cgacgggagt gagagtattt ttctgattca tacctatttg
      901 agcatttttg caaaaatgtt ggcttatgcc gttgtttcca acgatgatta tatcagtgat
      961 gatgagatgc gagaaatctt gaatggaagc atttttcaat cttataatat ttcaaatttc
     1021 gttgataacg atttctttca ttgggtcaat ggtgatcgcg attatctcaa attaaagaat
     1081 gtattccgtt tgattgcaca ggaaatctcg acttttaatt tttcgaacgt tgatgaggat
     1141 attctgaagg gggtatatca agaactcatt gatattgata cgcgtcattc tttaggtgaa
     1201 tattatactc ctgattggct ttgtgaacga atagtgaatg aatacgaatt taagaagacc
     1261 gataggattc tggatcctgc atgtgggagt ggttcattcc ttcgggcatt tatccataga
     1321 atcagggagt tgaatccaga tgctactgcc gaggaaatta acaatcagat ttatggcatt
     1381 gatattcatc cgctttccgt tcaaattgct aaaactactt tattgctggc tttgggtaag
     1441 ggtatttcaa gagcgagaag gccaattcat ttaaatatta tccttgccaa tactttactt
     1501 gctcccgaag gagtacagaa tgctttcggc aatgattttg tgatgcagat cgataaagat
     1561 aaatatatta tcaataccca gatttttgat aatgttgaag tattcgatgc ctttcttgat
     1621 atttgtgagg aattgtcgga gcagacctta aataaaaagg atatcgaact atcgttattt
     1681 gaagatattt tgaagcatca caccaatggt gggctttgtt cccaattggt cgagagtttt
     1741 tataaaatct atcagggagt taagagggtt aaagaaaaag gccgcaatgg catctggaaa
     1801 ttcattcttt caaatcttta taaacccttc ttccttgcaa atagatttga ttatgttata
     1861 ggtaatcctc cttggtttac ctatagttct atcaagaata aagaatatca agacttactt
     1921 gatgaacttg cggtaaaata taatgttaaa cctcaaaaag ttgctaatta tccgcatttg
     1981 gagattgctg ctatttttca atcatattgt agcagttatt ttttgaatga ttggggcaaa
     2041 caggcttttg tgcttccaag atcttttttt agtgccgatc atcacgaaaa ttcgagaagt
     2101 ggcattgcca aaggattccg cttgagtagt atatgggatt tagacaatgt ttctcctttg
     2161 tttaggattc cttgttgtgt attcttctct gaaaagaagc aaaaagaaag gataatgatg
     2221 tcctcgggct tagatgggaa aatatttgtg gggaaattac caacgcataa ttgtaattat
     2281 gcaatagcaa agaatatttt agttgaaact cctgtgaaat ggtattacat taggcaaggt
     2341 caatcttcgg cattatcgac gagaaaaatc gaaatgcaaa ggaatgtgaa tccatacaaa
     2401 aatcttttca aacaaggtgc aaccattgtg ccccgggcat tttactttgt tgaactcgat
     2461 cagcaaatgc ctgatgattt caacgatagg atcatcagtt taagaacctc gaaattcgtt
     2521 aaggccgact ccaaagaccc ttggaagctc gagttttcag gtaggatgga gagccgtttt
     2581 ttgttccgaa cagcaatttc gaagtctata ttgccatttc atttgtataa acccgatttt
     2641 gtggtccttc cccttattat tcaaattaat gagaatcaat ttaagaaaat tgagatacat
     2701 actgccgatg atattatgaa tatggggtat ctgaatgcat ctcgatggtt tcgcaatgcc
     2761 gaaagaattt ggaatatcaa taaaacggaa aagtcgaaga acatgtcaac cattggccga
     2821 cttaattttc ataatggctt aactcaacaa aatttgaatg cagaatattt agttttgtac
     2881 aactcgtcgg caaaagatgc caatgcatta gtagttaata gatcaagtct cgaccttgag
     2941 tttatatgcg aaagcaaatc gtatgtttat tatttaaa
//
LOCUS       DAORPD010000102         2978 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_5875_length_2978_cov_2.570304, whole genome shotgun sequence.
ACCESSION   DAORPD010000102 DAORPD010000000
VERSION     DAORPD010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5875_length_2978_cov_2.570304"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="PK976_01825"
     CDS             complement(<1..229)
                     /locus_tag="PK976_01825"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843285.1"
                     /note="hydrolyzes proteins to small peptides; with the
                     ATPase subunits ClpA or ClpX, ClpP degrades specific
                     substrates; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HRC66660.1"
                     /translation="MNNMNKDEFYKFATRHVGINPLALQKYIAVHDNYISPTIIEERQ
                     LNVATMDVFSRLMMDRIIFLGVPIDDYVANII"
     gene            complement(222..1652)
                     /locus_tag="PK976_01830"
     CDS             complement(222..1652)
                     /locus_tag="PK976_01830"
                     /inference="COORDINATES: protein motif:HMM:NF017508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HRC66661.1"
                     /translation="MEIIREDTGELTATLHLKLSEEDFLPQIVAELNKLQREAVIPGF
                     RKGKVPMEHIKKLYGYSLFFDYINKLLIETVIQYITENQLPILGYPLINQQAGSFNME
                     YYQGEEPEFLFDIAFTPEIEINFSEIQPVKYIKIVPTQTDIDRQIEKFREQFAELVEI
                     ETVDGEAIIEGELFEIDNSGSPKEDGYHQPIRFDIREESLPQEIKDKLLGLRKDEIID
                     FLFGSSEDLAKFIGISLSEAENLYKNLQLKIISISHKKLPELNQDFFHQVFPEQNIET
                     LEEFRAEVAKKINLNVLQEYSDRLFYLASMQMILNQTNIPLPKDFLKRWIATDENKDL
                     EEVDKGIDDYIESIRIDLLINYIIEKADIKIDEEDIRNHLAQKIVSSSFPYLQNNPNI
                     VEKISQYVDAAMKNEKNIENAENELRERKIIEFLKSNLPLEITELDSTTFMASITKDT
                     KKEDKEAQEEKSNEIMENNTENQNNE"
     gene            complement(1761..2054)
                     /locus_tag="PK976_01835"
     CDS             complement(1761..2054)
                     /locus_tag="PK976_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66662.1"
                     /translation="MASRKDTKKYIEYAIDEVISDCLNVLHLYPGKKEEEVYHLLREL
                     VNTRNNLVYRVNHIEGKDDPRAVKAHFRSIENDLNHQIEQAIEKLSNIVKSND"
     gene            complement(2109..2190)
                     /locus_tag="PK976_01840"
     tRNA            complement(2109..2190)
                     /locus_tag="PK976_01840"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2154..2156),aa:Leu,seq:tag)
     gene            2325..2546
                     /gene="yidD"
                     /locus_tag="PK976_01845"
                     /pseudo
     CDS             2325..2546
                     /gene="yidD"
                     /locus_tag="PK976_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008146842.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
BASE COUNT          820 a          545 c          521 g         1092 t
ORIGIN      
        1 gaataatatt ggccacataa tcatcgatgg gtactcctaa aaaaatgatt ctatccatca
       61 tcaacctcga gaacacgtcc attgtggcta cattgagttg acgttcctca ataattgtag
      121 gcgatatgta attatcatgt accgcaatgt atttctgcaa agccaaagga tttattccta
      181 catggcgagt tgcaaatttg taaaattcat ctttattcat attattcatt attttgattt
      241 tctgtattgt tttccattat ttcattactt ttttcttcct gagcctcttt atcttctttc
      301 ttcgtatctt ttgtaattga agccatgaat gttgttgaat ccagttctgt aatttcgagt
      361 ggaaggttcg attttaggaa ttctataatt ttacgttctc tcagttcgtt ctctgcattc
      421 tcaatatttt tttcattttt cattgctgca tccacgtact gtgatatttt ttctactata
      481 ttaggattat tctgaagata agggaaactc gaagatacaa tcttctgtgc taaatgattt
      541 ctgatgtctt cttcatcaat ttttatatct gctttttcaa taatataatt gattagtaag
      601 tctatacgaa ttgattcgat ataatcatca atacctttat caacttcttc taaatcttta
      661 ttttcgtcgg tggctatcca tcttttgaga aaatctttgg gcaggggaat attcgtttga
      721 ttaagtatca tttgcatgga agccaagtag aataatctgt cgctatattc ttgcaaaaca
      781 tttaaattaa ttttttttgc tacttcggct ctgaattctt ctaacgtttc tatgttttgt
      841 tcggggaaaa cttggtgaaa aaagtcctga tttagctcag gcaatttttt atgagaaatg
      901 ctaatgattt taagttgaag attcttgtat aaattctccg cttcgcttag gctaattcca
      961 ataaatttgg ctaaatcttc ggatgaacca aacagaaagt cgatgatttc atcttttctt
     1021 aaacctaata gcttatcctt aatttcttga ggaagggatt cctctctaat atcaaatctt
     1081 attggttgat gatatccatc ctcctttggc gagcctgaat tgtctatttc gaacagttct
     1141 ccctcgatga tagcctctcc atccacagtt tctatctcca ccaattcggc aaactgttct
     1201 ctaaatttct cgatttgacg atctatatcg gtttgcgtag ggacgatttt aatgtacttc
     1261 accggttgta tttcggagaa attaatttct atttcgggtg tgaatgctat atcgaaaaga
     1321 aattcgggtt cctcaccttg ataatactcc atattaaatg acccagcttg ctggttgatt
     1381 aacggatatc ctaagatagg caattggttt tctgttatat attgaatgac tgtttcaata
     1441 agtaatttat taatatagtc aaaaaagagt gaataaccat acaatttctt tatatgctcc
     1501 ataggcacct ttcctttgcg aaatccagga atgactgcct cacgttgtaa tttattaagt
     1561 tcggctacaa tttgaggaag aaaatcttct tcagataact tcaaatgaag tgttgcggtc
     1621 aattcacctg tatcttctcg aataatttcc atggataatt taagttttta ttttgaaagt
     1681 ttgctcgtaa atattgatgt ttaggtttgc cttttctctg tgtcttccca gaatttaggt
     1741 aaggcagtta taagcatttt tcaatcattc gacttaacaa tgttgcttaa tttttcaatg
     1801 gcttgttcta tctgatggtt taaatcgttt tctatgctcc tgaaatgagc tttaaccgca
     1861 cgagggtcat ctttgccttc gatatgattc acccggtaca caaggttgtt gcgggtattg
     1921 acaagttctc tcagaaggtg ataaacttct tcttcttttt tacccggata aagatgaagt
     1981 acatttaaac agtcagagat tacctcatcg atggcatatt caatatattt tttggtatct
     2041 tttctactgg ccattgggtt ttttaagttt taagtaagga tattaaacta ttagaaaata
     2101 tgttactgtg cggatgaagg gactcgaacc cccacgcctt tcggcaccag atcctaagtc
     2161 tggctcggct accaattacg acacatccgc atagggtttg caaaattaca ataatttata
     2221 agattggcaa ccgaattaaa gagaatggct ttcgttgctt aaatggcttt tgttcgaaga
     2281 ttttcatcgg aaggtattgc ttattttcag ctatttttgc aaacgtgctg aagcaaatac
     2341 taaaaactcc gttgattatt ctgataaggg tgtatcaatt tggtatttca ccatttacac
     2401 ccgccagttg caggcattgg cctagctgca gcacttatgc tatcgaagcc atcaaacggt
     2461 atggcatatt tcgaggtggg atgcttgcca tcaaccgtat tttgcggtgt catccgtggg
     2521 gaactagtgg catcgaccct gtcccacttt tcttattcca tcgcttttat tccaataaaa
     2581 aactaagttg taacaggttg aagcctcatc cggaaaattt gcaataagct gaaaatgtac
     2641 ccattgaatt gtctcgatga ttttctggct tcgaaatatc cttcgtaaga gattggaatt
     2701 ttaaagaact gagcctccta tttcattggt ttagcattgc tcgaatgcga ccaaaatagc
     2761 acgatgaatc tttgaaaaag tataaggagt ttgtcgacaa ttcgtaagtg ttcgaaaagt
     2821 agagatttta atttttattt gcttcctacc atcattccgc aagcagccga aatatcgtaa
     2881 ccagaagata ttctgatggt taccctgagg ccgtactcgt ttaatatttc tctgaagttt
     2941 tcgatggttc gaagacaagg tcttctgaaa tttgcccc
//
LOCUS       DAORPD010000103         2977 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_5878_length_2977_cov_2.380903, whole genome shotgun sequence.
ACCESSION   DAORPD010000103 DAORPD010000000
VERSION     DAORPD010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5878_length_2977_cov_2.380903"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1136
                     /locus_tag="PK976_01850"
     CDS             <1..1136
                     /locus_tag="PK976_01850"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HRC66663.1"
                     /translation="LNSPPPFCQPEVLLDIPKCKANIEKMVNISRQEGIIFRPHFKTH
                     QSKGIGRFFRQAGVSAITVSSVSMAKYFAEDGWNDITIAFPVNLREWEQIKSLASIVH
                     LSLLISDETSLQKISRLDFLSFHLNFFIELDTGQGRSGFEYSAEKILPLIRLADELQI
                     PIEGFLFHDGHTYKAHNTDEIRKIRAATLAMTAHLRHDLEVFYPGRKFLYSMGDTPSC
                     SIDNRYEGIDEMRPGNFVFYDLMQYDLGTCTQDEIAIRLVCPIVAVYSEQERAVIYGG
                     AIHLGKDFLTLPHNEICFGAICAHGDSIEWQGRYPMAWLSSLSQEHGVVKAPKDFLKK
                     IQPGDLLDVVPVHSCLMVSAALRIIGSDGTIFDTFNANFMKDT"
     gene            1178..2059
                     /locus_tag="PK976_01855"
     CDS             1178..2059
                     /locus_tag="PK976_01855"
                     /inference="COORDINATES: protein motif:HMM:NF024762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HRC66664.1"
                     /translation="MEKKKTIKELIALISMVDEPDEVVYGRIRQRIIDLGEEALPSLE
                     KAWENSFEEFTRLRIQELLDTIHFESIQQQLITWVNSNQDDLLEGAILLSRYHFHELD
                     INRITHNLGQIIQDVWLEMNDRMNPLEKIKVLNHILFDVHKFTPTKPPQYSLQDYLIP
                     NLIDSKRGSGTTLGILYLIIAQSLKLPVYGVNLPYNMIIAYLENNPGIYIYDPENDPV
                     LFYINPVFKGMIFIRKEIDRFLKDNKIEPQPQYFTACNNVQILQRLASEMRMMYDMEG
                     DSEKAERMDQIIAILKS"
     gene            2175..>2977
                     /locus_tag="PK976_01860"
     CDS             2175..>2977
                     /locus_tag="PK976_01860"
                     /inference="COORDINATES: protein motif:HMM:NF014759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HRC66665.1"
                     /translation="MPEQKLFLLDAMALIYRAFYAMVKTPRINSKGMNTGAVLGFANT
                     LWDVLKNENPTHIGVAFDMQAPTIRHEDFADYKANRQAMPEDLAASIPWIKELLRAFN
                     IPILEQEGYEADDIIGTLAVQAAEKGFKVYLMSTDKDLGQLVNENILLYRPARFGNGA
                     EILGMNEICQKYGIKNPTQLADLLGLWGDASDNIPGVPGIGETYAKKLIAQFDTIENL
                     IQNADKIENPKLKKKILSHTEEALFSKRLATIRLDAPVKFDEELFKRQAP"
BASE COUNT          898 a          592 c          618 g          869 t
ORIGIN      
        1 atttgaatag tcctccccca ttttgccagc ccgaagtttt gctcgatatt cctaaatgta
       61 aggccaatat cgaaaagatg gtgaacatca gcaggcagga agggattata tttcgtccac
      121 atttcaagac gcatcaaagt aagggaatag ggcgattttt caggcaggca ggagtaagtg
      181 ccattacagt ttcttcggtt agcatggcca aatattttgc cgaagatggc tggaacgaca
      241 tcaccatcgc attccctgta aatctccgcg aatgggaaca gattaagagc cttgcctcga
      301 tagtgcattt atcactactt atttcagacg agacatcttt acagaaaatc tcaagacttg
      361 attttttgag ctttcatttg aattttttca tcgaattaga cacagggcag gggagatctg
      421 gatttgaata ctcggctgag aaaattttac ctcttattcg attggccgat gaactccaaa
      481 tacccatcga aggtttcctg tttcacgatg ggcatactta taaggctcat aacactgatg
      541 agattagaaa aattcgcgcg gccacccttg ctatgacagc tcatttacgt catgatctcg
      601 aagtattcta cccaggaaga aaatttttgt attcgatggg agatacacct tcatgcagta
      661 ttgacaatcg ttatgaaggc atagacgaaa tgaggccggg taatttcgtc ttctacgatt
      721 tgatgcagta tgatttgggt acctgtacgc aggatgagat cgctattcgc ttggtttgtc
      781 ctattgtagc cgtatattct gaacaggaaa gagccgtgat ttatggcgga gccatacatt
      841 tagggaagga ttttttaact ttacctcaca acgagatatg ttttggggct atttgcgctc
      901 atggagattc tattgaatgg caagggcgat atccgatggc ttggctaagt tcgctctccc
      961 aagaacacgg cgttgtaaag gctccaaaag attttctcaa aaagattcag cccggcgatc
     1021 ttctcgatgt cgttcctgtg cattcttgcc tgatggtaag tgcagccctg agaataatag
     1081 gaagcgacgg tacaattttt gatactttta acgcaaactt catgaaagat acataatttc
     1141 aatttattga gccaacagga acttcttctc tatacttatg gaaaaaaaga aaactataaa
     1201 agaattgata gcattgataa gtatggtaga tgaacctgat gaagtagtgt atggaaggat
     1261 tcgtcagcgc attatcgacc tgggagaaga agccttgcca tcactcgaaa aagcttggga
     1321 aaattccttc gaagaattta ctcgtttacg tatccaagaa ttgcttgata cgattcattt
     1381 tgagagtatt cagcagcagt tgattacttg ggtaaattcg aatcaggatg atcttctcga
     1441 aggagcaata cttctttctc gttatcattt ccatgaactc gatatcaaca gaattacaca
     1501 caatctggga cagatcattc aggatgtatg gctcgaaatg aacgatagga tgaacccgct
     1561 cgaaaaaatc aaggtgctca atcatatttt gttcgatgtt cataaattta cacctaccaa
     1621 gcctcctcaa tattcccttc aagattatct cattcccaat ttaatcgatt cgaaacgtgg
     1681 aagtggtact actttgggca ttctatacct tatcattgcc caaagcctca aattgcccgt
     1741 ttatggggtg aatttgccct ataatatgat aattgcctat cttgagaata atccgggcat
     1801 ttatatttat gaccctgaaa acgaccccgt gcttttttat atcaatccgg tattcaaggg
     1861 aatgatattc attcgcaagg aaattgaccg ttttttgaaa gataataaga ttgaacctca
     1921 acctcaatat tttacggctt gcaacaacgt acagattctt caacgtttgg caagcgaaat
     1981 gcgcatgatg tacgatatgg agggtgacag tgaaaaagcc gaaagaatgg accagattat
     2041 tgccattttg aagtcataaa cggggaagat atttttcgat cttaggcaat tggcttaatt
     2101 atttgcattt tataagcctc catttttatt atgaaatcct ttccgtaaat ttgttttcga
     2161 aacttaacaa gaggatgcct gaacagaaat tgtttctact cgatgccatg gctttgattt
     2221 accgggcctt ttatgctatg gtgaaaacac ctcgtatcaa cagcaaagga atgaataccg
     2281 gtgctgtctt gggatttgcc aatacactat gggatgtact aaaaaatgaa aatcctactc
     2341 atattggtgt ggctttcgac atgcaagccc ctactattcg tcacgaagat tttgccgatt
     2401 ataaagccaa tcgtcaagca atgcccgaag atcttgccgc ttcgatacct tggattaagg
     2461 aactccttag ggctttcaac attcccatac tcgaacaaga aggatacgaa gccgatgata
     2521 ttataggtac cttggcagtg caggcagcag aaaaaggctt caaggtttat ctgatgagta
     2581 ccgataaaga tttaggccag ttggtaaatg aaaatatact gctctaccgg cctgcacgat
     2641 tcggtaatgg cgccgaaata ctcggaatga acgagatatg tcagaaatat gggataaaaa
     2701 atcccaccca gcttgcagat cttttgggcc tttggggcga tgcttccgat aatatacccg
     2761 gtgtgcccgg cattggcgaa acttatgcga aaaaactcat tgcacaattc gatactatcg
     2821 aaaatttaat tcaaaatgcc gataagatag aaaacccaaa actcaagaag aaaattcttt
     2881 ctcacactga agaagcctta ttttcgaaac gacttgcaac tattcgtttg gatgctcccg
     2941 taaaatttga cgaggaactt tttaaacgcc aggctcc
//
LOCUS       DAORPD010000104         2977 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_5879_length_2977_cov_1.911704, whole genome shotgun sequence.
ACCESSION   DAORPD010000104 DAORPD010000000
VERSION     DAORPD010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5879_length_2977_cov_1.911704"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1878
                     /locus_tag="PK976_01865"
     CDS             <1..1878
                     /locus_tag="PK976_01865"
                     /inference="COORDINATES: protein motif:HMM:NF014589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HRC66666.1"
                     /translation="KHHEGFPHLAINYCLNVANIHDINNVHCIVMNQYTKSSHEIELL
                     YLGYKNNLVINPSHHLLHAYYAYCASSFINAAVLIVDGSGYNYGEYVVRNSPHIGPAP
                     KFSEMDEALTLFHIDKGNIDLVDKTWGLWNSSEPYYRFPSLGHMFSVASQYIFGDWVH
                     AGKTMGLAPYGDSSGIPFQIITLLDDGIEIDVDWILKLPKPSYNLPADEVKIKRDLAA
                     KVQEELENAILHIVRIAHRKIPSKNLCLSGGVALNSVANGLIVRDGPFEKLFIAPAAS
                     DGGIAIGAALYGFCQLNSSLPASFDASDFYGKEYEINEISELVQNDSRIIYELVDEPG
                     VRAASDIAEGKIVGWFEGGSEFGPRALGHRSILCDPRLPNMKDMLNSRVKYREGFRPY
                     AASVLKEHTSEYFAISEENPYMLVVTPVHPEKRSVIPAVCHVDGTCRIQTVDINYKGQ
                     FRQLIEHFFRLTSVPLVLNTSFNIRGEPIVETPSEALACFLGSGIDTLYIGPYRISKI
                     ALDENSEKDNIHKLIPCLNDKLILIKENLASKGKWMSNRTIIRTRTGYQLPLEEPLLR
                     LLELIDGRKTGKELLYEINNILGTPWTEDEMLLNLYHLQKHGLICFIARESTVAIPLS
                     N"
     gene            1915..2898
                     /locus_tag="PK976_01870"
     CDS             1915..2898
                     /locus_tag="PK976_01870"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRC66667.1"
                     /translation="MDTNTVDVSVVIVNWNTRDLLHQTLETLYKESHDVNFETIVVDN
                     GSTDGSIELVEEKWSNVRLLTLPENKGFAVGNNIGFREVKGRYVLLLNSDTIVLASTL
                     YEMVRFLDAHPEAGCVGCRHLNPDGTLQRSVDNFPSLINDFLSYSELHRLPLLQPYLA
                     KRFLWWGDHNCTRPVDWVNGACMMVRSNVIQQIGGLDEGYFIYAEEIDWCYRMHKAGW
                     LVYFLSDAEIIHIGGQAMNQAADKRIILKYKGQYRFYRKHYPLWKYICLRLIVTGIAI
                     PRIALLFIFYVFSRLQKETDHNQWEILTQEPVKTGPGVMLNAWWKILWLPF"
BASE COUNT          879 a          519 c          663 g          916 t
ORIGIN      
        1 aaacatcacg agggtttccc tcaccttgcg ataaactatt gtcttaacgt ggccaatata
       61 catgatatta ataacgttca ttgcattgtt atgaatcaat acactaagtc atcacacgag
      121 attgaactcc tttatttggg ttataaaaac aaccttgtaa taaatccctc acatcacctt
      181 ttgcatgctt attacgctta ctgtgcatct agttttatta atgccgcggt tctgatcgtt
      241 gatggaagtg gatacaatta tggggaatac gttgttcgaa atagtcccca tattgggcca
      301 gcgccgaaat tttctgaaat ggatgaagct ctcaccttat tccatattga taaaggcaat
      361 atagatcttg tagataagac ttggggttta tggaattcga gcgagccata ttaccgtttc
      421 cctagtctgg ggcatatgtt ctccgttgct tcacaatata tttttggcga ctgggttcat
      481 gctggaaaga caatggggct ggcaccatat ggagacagca gcggaattcc tttccaaata
      541 attacactac tggatgatgg tattgagatt gatgtcgatt ggatattgaa attgcctaag
      601 ccttcataca acttaccagc ggatgaagta aagataaaaa gagatttagc agccaaagta
      661 caagaagagt tagaaaatgc tattcttcac atcgtacgaa ttgcacacag aaaaatccca
      721 tccaaaaact tgtgcctttc gggcggtgtg gctttaaata gtgtagctaa tgggctgata
      781 gttcgcgatg ggccttttga aaaattattc attgctcctg cggctagtga tggcggaatt
      841 gcaattggcg ctgctcttta tggcttttgt cagttgaatt catcactacc agcaagtttc
      901 gatgccagtg atttttatgg caaagagtat gaaataaacg agatatcaga actggttcag
      961 aatgattctc ggattattta cgagttagtt gatgaacctg gtgttcgagc cgcatctgat
     1021 attgcggagg ggaaaattgt tggatggttt gaaggtggca gcgaatttgg accgcgtgca
     1081 cttggacatc gaagtattct ttgtgatccg aggttgccca atatgaaaga tatgctaaat
     1141 agccgagtta aatatcgtga gggatttcgc ccttatgcag ccagtgttct aaaggaacat
     1201 acgtctgaat attttgcaat aagtgaagaa aacccttata tgctagttgt aactcctgtg
     1261 catcccgaaa aaagatctgt aattcccgct gtttgtcatg ttgatggcac atgccgaatc
     1321 caaactgtgg acataaacta caaggggcaa ttcaggcaac ttattgagca ttttttcagg
     1381 ttgacatccg ttccattagt gttaaacacg tctttcaaca tacgaggcga gccaatagtg
     1441 gaaactccat cagaggcctt ggcctgcttt ttaggttcgg ggatcgatac attgtatatc
     1501 ggcccctacc gaatttctaa aattgctctt gatgagaatt ctgaaaagga taacatccat
     1561 aaattaattc cttgtttgaa tgataagctt attttgatca aagaaaactt ggcttctaaa
     1621 ggtaaatgga tgagcaatcg cacgattatt cgcactagga ccggttacca attgccttta
     1681 gaagaacccc ttctaagatt attggaattg attgatggaa ggaaaacagg taaggaatta
     1741 ttgtacgaaa tcaataatat tctaggaact ccctggacag aagatgaaat gctattaaat
     1801 ctataccact tgcaaaaaca tggcctgata tgcttcattg cgagggagag cacagttgcc
     1861 atcccgttga gtaactaaac gtctgagata aaggggtaca ctatgaggta aataatggac
     1921 acaaatactg tagatgtatc agttgtgatt gttaattgga atacacgtga tttattacat
     1981 caaacacttg agactttata caaggaaagc catgatgtaa attttgaaac tatagttgtg
     2041 gacaatggtt caacggatgg cagcattgag cttgttgaag aaaaatggag caatgttcgt
     2101 ttactaacat tgccagaaaa caaggggttt gcagtgggta ataatattgg gtttagagag
     2161 gtgaaggggc gttatgttct gctattaaat agcgatacta tagtactggc ctcgaccctg
     2221 tatgaaatgg ttcgtttttt ggatgcgcac cccgaggctg gttgtgttgg atgtcgtcat
     2281 ctgaatcccg atggtacact tcaacgatcc gttgataatt tcccgagttt aattaatgat
     2341 tttttaagtt attcagaatt gcatcgatta cccttattac agccttattt agccaaacgc
     2401 tttctatggt ggggtgatca taactgtact cgtcccgtgg attgggtaaa tggtgcttgc
     2461 atgatggtac gtagtaatgt tattcagcaa ataggagggc ttgatgaggg atattttatt
     2521 tacgcagagg agattgattg gtgttatcgc atgcataaag ccggttggct agtttatttt
     2581 ttatctgatg cggaaattat acacatcggt gggcaagcga tgaatcaggc ggcagataaa
     2641 cggataatac taaaatacaa aggacaatat cgtttttatc gtaagcatta ccctctatgg
     2701 aagtatattt gtttacggtt aattgttacg ggcattgcta ttcctcggat tgcgctactt
     2761 tttattttct acgtattttc gcggttgcaa aaagaaacag atcataacca atgggaaatc
     2821 ctcacgcaag agccagtgaa aactggccca ggagttatgc ttaacgcttg gtggaagatt
     2881 ttatggcttc cattttaggg atatgatatt ctaatcatat tactatgcaa ttagggttgc
     2941 tttgagaagt acacatttct attcaaatca aatatta
//
LOCUS       DAORPD010000105         2974 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_5888_length_2974_cov_2.413840, whole genome shotgun sequence.
ACCESSION   DAORPD010000105 DAORPD010000000
VERSION     DAORPD010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5888_length_2974_cov_2.413840"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1238)
                     /gene="fabF"
                     /locus_tag="PK976_01875"
     CDS             complement(<1..1238)
                     /gene="fabF"
                     /locus_tag="PK976_01875"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005645359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HRC66668.1"
                     /translation="MGLRRVVISGLGALTPIGNDVNTLWASLVDGVSGAGPITRFNAE
                     KFKTKFACEVKGYDPLNFFDKKEVRKYDLFSQFGIVAADEAINDSGFKQGNYNPERVG
                     VIWASGIGGIMTLTEEIRAYVTGDGTPRFSPFFIPKMISDITSGHIAIKHGFMGPNFS
                     TVSACASSANAIVDAFNNIQRGKADIIITGGSEASISEAGIGGFNAMHALSTLNDDPR
                     AASRPFDKDRDGFVVAEGAAALVLEDMEHALARGARIYCEIAGAGLSADAYHITSPHP
                     EGAGAAIAMRLAIEDAKLQPSDLDHINTHGTSTPQGDIAEVKAIMKVFGEHVYNLNIN
                     STKSMTGHLLGAAGAIEALATVLAVYYDIVPPTINHFTDDPEINPALNFTFHKAQQRT
                     VNAAISNTFGFGGHNASILM"
     gene            complement(1261..1500)
                     /locus_tag="PK976_01880"
     CDS             complement(1261..1500)
                     /locus_tag="PK976_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRC66669.1"
                     /translation="MSDIENRVKAIIADKLNVDESEVTPEASFTNDLGADSLDTVELI
                     MEFEKEFNIAIPDDQAEKIGTVGEAIKFIEGNIKS"
     gene            2033..>2974
                     /locus_tag="PK976_01885"
     CDS             2033..>2974
                     /locus_tag="PK976_01885"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HRC66670.1"
                     /translation="MESLYIVIVGFLFLLAIVDLIVGVGNDAVNFLNSAVGSKAAPFK
                     IILAVAFVGVVTGTLFSSGMMEIARSGIFNPSMFYFNEVMYIFLAVMITDIVMLDAFN
                     NLGLPTSTTVSLIFELLGASVAVAVMKTIIDPMAVSNVAEYLNTAKVLAIIAGILLSV
                     VIAFFFGTVIQYISRLLFTFRYKQKLPYLGGIWGGISFTIIIYFLILKGIRGAAFIDE
                     TTIHYIESHTATLMLLSIVGFTIIFQLLMWLFKINVLKIVVLTGTFALAMAFAGNDLV
                     NFIGVPMAGYESYRIYIQSGVTDPNSLFMGALAQPIKS"
BASE COUNT          823 a          581 c          589 g          981 t
ORIGIN      
        1 tgcatgagga tggaggcatt atgaccgcca aatccgaaag tattgctgat agcagcattc
       61 acagttcttt gttgggcctt atgaaaggta aaattaagag ccgggttgat ttcgggatcg
      121 tcagtaaaat ggttgatggt aggcggcaca atgtcataat aaacagccaa taccgtagca
      181 agtgcttcga tggctccggc ggctccaagc aaatggccgg tcattgattt ggtcgagttg
      241 atattgaggt tgtaaacatg ttcaccgaaa actttcatta tggctttcac ctcggctata
      301 tcaccttgag gagtagaagt accatgggta ttgatatgat ctaggtcgga aggttgcagt
      361 tttgcatctt caatggctaa tctcatggca attgcagctc ctgctccctc gggatgagga
      421 gaagtaatat ggtaagcgtc ggccgatagc ccagctccag ctatctcaca ataaatcctg
      481 gctcctctgg ctagggcatg ttccatatct tcgagaacga gtgctgcagc accttctgca
      541 actacaaaac catctctgtc tttgtcgaaa ggtctcgaag ccgctcgagg atcatcatta
      601 agagtagaaa gtgcgtgcat ggcattaaac ccacctattc ctgcttcgct tatggaagcc
      661 tccgacccac ctgtaataat gatatcggcc tttcctcttt gaatgttgtt gaaagcatct
      721 actatggcat tcgcagatga agcacaggct gaaacagtag aaaaattcgg tcccataaat
      781 ccatgcttta tggcaatatg ccctgaggta atgtcggata tcatcttcgg aataaaaaat
      841 ggactaaaac ggggagttcc atcccctgta acataagccc tgatttcttc cgttaaggtc
      901 ataatgccac ctatacctga agcccagatc accccaaccc tctcgggatt ataattccct
      961 tgcttgaaac cagaatcatt gatagcttca tcggcagcta caataccaaa ttgcgaaaac
     1021 aagtcatatt tgcgaacttc tttcttgtcg aaaaaattca gaggatcata gcccttgact
     1081 tcacacgcaa atttcgtttt gaacttttcc gcattgaatc ttgtaatagg gccagcgcca
     1141 ctgacaccgt caaccaatga tgcccatagg gtatttacat cattccctat cggagtcaga
     1201 gcgcctaacc cgctaataac aactcgtctt aatcccatca ttgaacgtta aataggttcc
     1261 tcagctcttt atgtttcctt cgatgaattt tattgcctcg cctacagtgc ctattttctc
     1321 agcctggtcg tcaggaatgg caatattgaa ttctttttca aattccataa tgagttccac
     1381 ggtatcgagg gagtcggccc ctaaatcatt ggtgaagctg gcctcggggg ttacttcact
     1441 ttcgtcaaca tttagcttat cagcaatgat agcttttact ctgttttcaa tgtctgacat
     1501 aagtgtggtt ttttggttaa acaaggttgc aaagaaaata cgattttaca aattgtgcaa
     1561 attttaaatg ttaataaaac ctaaaatttg attcaataaa ttgaaataca atatattgat
     1621 aagtttagcc atttggttaa aatgaatgat gtttccactc attttactcc ttttatttca
     1681 caatctgaac gttattcggc ttgttttatc cataaaatct tcctcccaaa taataaactt
     1741 caagtttaaa aaagccggcc tgaaaatatg aagtgtttcg gattgatctt ctatttgatt
     1801 gtttatcatc ttgttgaagg agtgagttat aagactattt atttggttgg aggtcaacac
     1861 gatcgaatct cattttaccc tatcaattgc aaaagaattc tttgttttcc aatctttctt
     1921 ctatcatttc acgtcgatag tattttaatg gattacatta acaatttctt aaaatggaaa
     1981 taatctacag aacaaatgag taatataatt ttgtggccaa attaatcatt tgatggaaag
     2041 tctatatatt gtcatagtcg ggtttttgtt tctattagcc attgtggacc ttattgttgg
     2101 agtggggaat gatgccgtaa attttctcaa ttcggcagta ggttcaaagg cagcgccctt
     2161 taagataatt ttggcggtgg cttttgttgg agttgttaca ggaactctat tttcgagtgg
     2221 gatgatggaa atagcgcgta gtggaatttt caatccttcc atgttttatt ttaatgaggt
     2281 gatgtacatt tttctggcag ttatgatcac cgacattgta atgctggatg ctttcaataa
     2341 cttaggattg cccacttcaa ctactgtttc attaattttt gaacttttag gtgcatcagt
     2401 agcagttgcg gtcatgaaaa ccataatcga tcctatggca gtctctaatg tggcagaata
     2461 cctcaatact gccaaggttc tggcgattat tgcaggtata ttattatcgg tagtaatagc
     2521 attcttcttt ggaacagtca ttcaatatat aagccgtttg ctttttactt tccgttataa
     2581 gcaaaagctg ccttacttag gaggaatctg gggaggaatt tcatttacca tcatcattta
     2641 ttttttaata ttgaaaggta ttcgtggagc ggcttttatt gatgaaacta ctattcatta
     2701 catagaatct catactgcta ccttaatgct tttaagcata gtaggtttca caataatttt
     2761 tcagttgttg atgtggcttt ttaaaatcaa tgttcttaag attgttgttt taacaggcac
     2821 tttcgcactt gccatggctt ttgcggggaa tgatctggtc aatttcattg gtgttcctat
     2881 ggctggctac gaatcgtata gaatatatat tcaatcaggg gttaccgatc ctaattctct
     2941 atttatgggg gctttagctc aacctatcaa gagc
//
LOCUS       DAORPD010000106         2972 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_5894_length_2972_cov_2.914981, whole genome shotgun sequence.
ACCESSION   DAORPD010000106 DAORPD010000000
VERSION     DAORPD010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5894_length_2972_cov_2.914981"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="PK976_01890"
     CDS             complement(<1..419)
                     /locus_tag="PK976_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66671.1"
                     /translation="MNKSVIRVVIILSGISLVSLVILQFVWFKNMLQMRENEFDRDVM
                     AAVAETARRLERDQTMFFVSNRLRMQALTDTILASTHPKNRTATINKTSTNMNSILPD
                     SLPPRRRVNLKHRQWVVQQNARGEIIVETENQLDTLTS"
     gene            554..1639
                     /locus_tag="PK976_01895"
     CDS             554..1639
                     /locus_tag="PK976_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC66672.1"
                     /translation="MKSLVRILNCLMIPFVLSLILGFSSLAFGQNQPPFSPPSKNQNL
                     SPEFQSLPDKANWEEIMNELENLYMLFPAEEWNDEPMEEYLSDFRDFMQREAPPIPEN
                     LMEVKRHLEMAMRQLDEAIRQMPEFPRPDLKGWKFREHHLSDFHSYGFHNLPQGCPYG
                     TFRDDSLVMDTAGKSIKIIRKMIVGRDTLTKEIECIRMSTPNGRVDTLIIFKDGRKTI
                     DSIVWNRSRQRADLRSFRQTPQSWRYSERKPKSRTLYMVDLNSDDLNKLSHSALKPSQ
                     VKEPLDLDKLSVNKSVRGKIRLRFTTEQSGDIEVSVFDEQGSILHHEILKKFSGNYDR
                     IITLLPQPVYYLKIQQGKKVLQKKITE"
     gene            2187..2534
                     /locus_tag="PK976_01900"
     CDS             2187..2534
                     /locus_tag="PK976_01900"
                     /inference="COORDINATES: protein motif:HMM:NF025304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRC66673.1"
                     /translation="MKHLTVEQRYEIWAMILQEWKQKDIALAIGKDKSVVSRELKLNC
                     DNRNREYRADLAQRKYERRQKNKCKNLRFTEEVKQYVDGYLVKDFSPEQIAGRIALEG
                     RECVSHERIYQYV"
     gene            2553..2972
                     /locus_tag="PK976_01905"
                     /pseudo
     CDS             2553..2972
                     /locus_tag="PK976_01905"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT         1017 a          524 c          574 g          857 t
ORIGIN      
        1 gaagttaatg tatccaattg gttctcagtt tcaacaatta tttcacctct ggcattttgc
       61 tgcaccaccc attgacgatg ttttaggtta acccttcgac gaggaggaag actatcgggc
      121 agaattgaat tcatgtttgt actggtttta ttgatcgtgg ccgtacgatt tttcggatga
      181 gttgaggcaa ggatcgtatc ggttaaagct tgcattctga gtctgttcga aacaaaaaac
      241 atggtctgat cgcgttcgag ccgccgtgca gtttcggcaa cggctgccat tacatctcta
      301 tcgaactcgt tctcacgcat ttgcagcata ttcttgaacc atacaaactg cagtatcacc
      361 aagcttacca gcgaaatgcc actcaatata ataacaaccc gtataactga tttattcata
      421 atgcaaaatt aaattgattt gcccgcctat ttggctattt aacttttctt taacaaactt
      481 taacggcata ttaacataat gattatgaac agcttggtaa ttttgttcgt tcaataatta
      541 aaacaaaaat atcatgaaat ctttagttcg tattttaaat tgcctgatga ttcctttcgt
      601 attaagctta attttaggct ttagttcgct ggcatttgga cagaatcagc caccattttc
      661 tccaccatct aaaaatcaga atctttcacc cgaatttcag tcattgccag ataaggcaaa
      721 ctgggaagaa atcatgaacg aattggagaa tttgtacatg cttttcccag ccgaagaatg
      781 gaatgatgaa ccgatggaag aatatctttc tgatttcagg gattttatgc aaagagaagc
      841 tccccctatc cctgaaaatc tcatggaagt taagcgacat ctcgaaatgg caatgcgtca
      901 attagacgaa gccattcgtc aaatgcctga attccctcgt cctgacctta agggttggaa
      961 gtttagagaa catcatttgt ctgattttca ttcttatggt tttcacaact tgccccaagg
     1021 atgtccttac ggaactttta gagatgattc cttagttatg gatactgccg gaaaatcaat
     1081 taaaattatt agaaagatga ttgtaggacg cgataccctg accaaggaaa tcgaatgtat
     1141 tcgcatgtct actcctaatg gtcgagtaga tactcttatt attttcaagg atggtagaaa
     1201 gacaattgat agtattgtgt ggaatcgctc ccggcaaaga gccgatttac gttccttccg
     1261 ccaaactccg caaagctgga gatattcaga aaggaaaccg aaatccagaa ccctgtatat
     1321 ggttgatttg aactccgatg atctgaacaa actctcccat tcggcgttga aaccctccca
     1381 ggtcaaagaa cccttagacc tcgacaaact cagcgtaaat aaatcggttc gcggtaaaat
     1441 acgtctccgc tttaccacag agcaatcagg agatatagaa gtatcggttt tcgacgaaca
     1501 aggcagcata cttcatcatg aaatcttgaa aaaattctca ggaaattatg atcgaatcat
     1561 cacacttctt ccgcaaccgg tttattattt gaagattcag caagggaaaa aagtgctgca
     1621 aaaaaagatt acagaataat ttcaatactt tggaagcata aaatatcttc gccatatcat
     1681 ttgacgcaaa gcttcgatcg aaaaatcttt gttttgaatt cttaattaac cttatcgcct
     1741 tgatttgttc ccaacccttc atagtgcggt taaaaaacaa gcaataaggt taaatatttt
     1801 gagtcggatc attgtaaaat ttttcctaat atttataact aattgataaa caaccatcaa
     1861 aatatgatat ttacttgcat ttgataaata agtatatcat ttgtaattgg gagatattat
     1921 tagaattgag aaatattttt tctataagtt tatatctaca acatgagata aaatattaaa
     1981 tttcaattct ttgcatgata aattggtcat aagtcattca aaaaaggaat ttatcttgta
     2041 aattcaagta attaatgcaa tttttttagg ggaaataaaa gataaagaga aattattttt
     2101 caaggcaaag gagaaaaaag atttcccctt tgccttgatg gaataaatgc ttttctttgg
     2161 tctcttcccc ttaaaaagtt acaaagatga aacatttaac cgtagaacaa agatacgaaa
     2221 tatgggcaat gatactacaa gaatggaaac aaaaagacat agcgttggct atcgggaaag
     2281 acaaatcggt tgtatcacgt gagctcaagc ttaattgcga taatcgaaac agggaataca
     2341 gagcagattt agcacaacga aaatacgaaa gaaggcagaa gaataaatgc aaaaatttac
     2401 gttttacaga agaagtcaaa caatatgtag atgggtatct tgtaaaagat ttcagtcctg
     2461 aacaaatagc aggaagaatt gcattggaag gcagggaatg tgtcagtcat gaacgaattt
     2521 atcaatatgt atagaagaat aaaaaagaag gtggtgatct ttatactcac cttaggcata
     2581 agggacgcag atatcgaaaa cgtggaaacg ccaaagacag cagaggaatt attaaagaga
     2641 gggtcgacat aagtcaaagg ccagagatgg tcgaaagaaa agaaagattg ggcgacttgg
     2701 aaatcaatac gatgataggt caaaatcaca agggagcgtt gttgacgatc aacgacagag
     2761 taattagtta tgtcgcaatt gaaaagctca atagcaagga tgccaaagag cttgctcaaa
     2821 atgtcataga acgattgtct cccagtaagg gaaagattca tacgatcacc gccgataacg
     2881 gaaaagagtt tgtcgagcat aaaatgatta gctcagaatt ggaagtggat ttcttttttg
     2941 ctaaacccta tcattcatgg gaaggagggg tt
//
LOCUS       DAORPD010000107         2966 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_5910_length_2966_cov_2.250086, whole genome shotgun sequence.
ACCESSION   DAORPD010000107 DAORPD010000000
VERSION     DAORPD010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5910_length_2966_cov_2.250086"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="PK976_01910"
     CDS             complement(<1..488)
                     /locus_tag="PK976_01910"
                     /inference="COORDINATES: protein motif:HMM:NF024489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66674.1"
                     /translation="MSKKNEYIKSTDNLEDGLFINREISWLQFNERVLQEASDPTTPL
                     LERIKFLGIFSNNRDEFFRVRVATIHRMNMLMQKKELDYRISFDPRKLLSEIYSIVEN
                     QEKRFTAVYQDLTRQMKKNGIFIVNEQELTHEEGKYVRSFFNQNIRSYVFPIMLNNVK
                     NLT"
     gene            complement(526..1026)
                     /locus_tag="PK976_01915"
     CDS             complement(526..1026)
                     /locus_tag="PK976_01915"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRC66675.1"
                     /translation="MKTLIVARHAKSSWDFPQLKDEDRPLLSKGKKRISKIILSLQKE
                     KIARPGLIISSHAVRAFETSRIFASFFQYPENQIQVSQTVYYGNKDHITDLFYSIDDD
                     IDSVMIVGHNPNILAFINQFLLDPLDGLPTSGVVILELPIESWTELAGAKASTIQKIF
                     PWESNK"
     gene            complement(1205..2602)
                     /gene="lpdA"
                     /locus_tag="PK976_01920"
     CDS             complement(1205..2602)
                     /gene="lpdA"
                     /locus_tag="PK976_01920"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HRC66676.1"
                     /translation="MDCQYDVIVIGSGPGGYVAGIRAAQLGLKTAVVEKAELGGVCLN
                     WGCIPTKALIKSAQVYNYISQASDYGIKIEGSTRLDFEGMVKRSREVAATMSNGIQYL
                     FRKNKVEHIKGFGKINPGHEVEVISEDGTLKNYNARHIILATGARSRELPNLRQDGKF
                     IIGYREALTLPIQPQSMVIIGSGAIGSEFAFFYNSIGTKVTLVEFMPNVVPLEDEEVS
                     KQLERNFKKAGIKVMTESTVEQVEVKDHHCVVTINTKKGEETLDTDIVLSAVGIAPNI
                     EGIGIEETGIRTEKGKIVVDEFYRTNVEGYYAIGDIIPTPALAHVASHEGIICVEKIA
                     GHEPTPLNYGNIPACTYTQPEVASLGITEKVAREAGYEIKTGKFPYSASGKATAVGHR
                     DGFVKVIIEAKYGKILGVHMIGDNVTEVIAEAVVARKLESTGREIIDSVHPHPTMSEA
                     FMEAMAAAYGEVIHI"
BASE COUNT          809 a          615 c          554 g          988 t
ORIGIN      
        1 gtcagattct ttacattgtt cagcatgata ggaaaaacat acgatcgaat gttctggtta
       61 aagaagcttc ttacatattt cccttcttcg tgcgtaagtt cctgttcatt tacaatgaaa
      121 atcccatttt ttttcatttg acgggttaaa tcctgataaa cagcggtaaa ccttttttcc
      181 tgattttcga ctatactgta tatttctgac aataacttcc taggatcgaa actaattcta
      241 taatcgagtt cttttttttg catcagcatg ttcattcgat gaatcgtagc taccctcacc
      301 cgaaaaaatt catcccgatt attggaaaaa atgcccagga actttatcct ttccagtaag
      361 ggggtggttg gatcggaagc ttcttgcaaa actctttcgt tgaattgcag ccagcttatt
      421 tcccgattga taaataatcc atcctccaaa ttatctgtag actttatgta ctcatttttt
      481 ttactcattg ccatgctgat gtaaaatata aaattatgtt tatacctatt tattgctttc
      541 ccaggggaat attttctgta ttgtactggc ttttgctcct gccaattcgg tccaactctc
      601 gatgggcaat tcaagaatga cgacccccga ggttggcaac ccatccaagg gatcgagaag
      661 aaactggttg atgaatgcaa ggatattagg gttgtgcccg actatcatca cactgtcgat
      721 gtcatcgtcg atggaataaa aaagatcggt aatatggtcc ttatttccgt agtaaacggt
      781 ttggctgact tgaatctgat tttcgggata ttgaaagaat gaagcaaata ttctggacgt
      841 ttcaaaggct cttaccgcat gactcgaaat gatcagaccg ggtctggcaa ttttttcctt
      901 ttgtaaggat aggatgatct tgctgattcg ttttttccct ttgctcaaca atggcctgtc
      961 ctcatccttg agctgaggaa aatcccatga ggattttgca tgccgggcaa caataagtgt
     1021 tttcatgggc tataagtttt tcttaaatgt tagaatcttt acaaatttag taaatgcccc
     1081 aaagaattaa tctttagaaa atttttactc tttaaaactt cgagagacga atgattgcct
     1141 taacaagctc gacaagtgga ccacacgaag gtaatgctca gagcatatta atacggatgt
     1201 tttatcaaat atgaatgact tccccatagg ctgctgccat agcttccata aaggcttccg
     1261 acatggtagg atgaggatgt acgctgtcga tgatttctct gccagtactt tcgagctttc
     1321 tggctacaac ggcttcggca atcacttcag taacattgtc gccaatcatg tgaacgccta
     1381 atattttgcc atattttgct tctatgatta cttttacgaa tccatcacga tgaccaactg
     1441 cggtggcttt cccagaggcc gaatagggga actttcctgt tttgatctca tatccggctt
     1501 cacgggctac tttttctgtt atccctaacg aggctacttc gggctgagtg tatgtacaag
     1561 ctggaatgtt tccataattt aaaggtgtag gctcatggcc tgcaatcttc tccacacaaa
     1621 tgatgccttc gtgtgatgca acatgggcta atgccggggt aggaataata tctcctatgg
     1681 catagtagcc ttcgacattg gtacggtaaa attcatccac cactattttc cctttttctg
     1741 ttctaattcc ggtttcctct atgccaattc cttcaatatt aggtgcaatc cctactgccg
     1801 acaatacaat gtcagtgtcg agggtttctt cgcccttttt ggtgtttatg gttacgacgc
     1861 aatgatgatc cttcacttct acctgttcaa cggtcgattc ggtcatcacc ttgatacctg
     1921 ctttcttgaa attacgttcc agctgttttg aaacttcttc atcctctaat ggtactacat
     1981 tgggcatgaa ctcaaccaga gttactttcg tgccaatcga gttatagaaa aaggcaaact
     2041 ccgaacctat tgctcctgat cctataataa ccatcgactg aggttgaata ggtaaagtta
     2101 atgcttcgcg atagccaata atgaatttgc catcctgacg aagattagga agctcacgcg
     2161 aacgggcacc tgttgctaaa ataatatgtc ttgcattata attttttaat gttccatctt
     2221 cggaaatcac ctctacttca tgccctggat ttatttttcc gaaacctttt atgtgttcga
     2281 ctttgttttt cctaaaaagg tattgtatgc cattgctcat tgtagcagca acctcacgcg
     2341 aacgtttcac cataccttcg aaatcgagcc tggtactgcc ttctattttg attccgtagt
     2401 ccgatgcctg ggaaatgtag ttatatacct gagcgctttt aataagtgct ttcgtgggaa
     2461 tgcatcccca gttgagacac acccctccca attctgcctt ttcgactact gccgttttca
     2521 accctaactg agccgcacga attccagcca cataaccacc tggcccactg cctattacta
     2581 ttacgtcgta ttgacaatcc ataatatgtt gattttattt gagatagttc gttttttatt
     2641 gcaaatttat atatccttgc gaaatttcat cggatgatta agctatctag aataaatgat
     2701 atgttttaat ataacgatct ttaaatagat ttggtttgta aattttttta ttagggtgtg
     2761 actgctctga gtttttatat tttcagccag aaaagaagaa taccaaagca tcgtcattgg
     2821 ctggagattt ctgaaatgcc gataaaaacg aagtgtaaat catttcagta gaattcataa
     2881 aaactgtgta aaggcttatt ttcaggcatt aaaggaaata tttgtaaatg aatataatag
     2941 ccgaatggaa tagaaagaga aatttt
//
LOCUS       DAORPD010000108         2961 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_5932_length_2961_cov_2.471438, whole genome shotgun sequence.
ACCESSION   DAORPD010000108 DAORPD010000000
VERSION     DAORPD010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5932_length_2961_cov_2.471438"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..1359)
                     /locus_tag="PK976_01925"
     CDS             complement(112..1359)
                     /locus_tag="PK976_01925"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HRC66677.1"
                     /translation="MMNRLILTYRPIPSKVSIQLPYSKSISNRLLIIQALAGDTFEIA
                     HLSDADDTRVMAEAIRRYEEGFDVINVAEAGTAARFLTAYFALRAKPVILTGSGNLLR
                     RPISALVNTLRSMGAMIEYLDTPDRLPLHFKGGQLHGGNIQINADISSQFVSALLMIA
                     PMLPDPLNIHLINQEVSMPYVDLTIQCMRRCGAKARRLKNNMIEVDNSGYNFSGSIAM
                     EPDWSSAAPWFAISALQPSSEFLLENLDMNSLQADKLLIEAFKPMGVVTTSTHEGLLL
                     KWRGGVAKEMYFDFRNCPDIALSVIVTAAALNIDGVFANLSTLRFKETDRYQALKEQL
                     LRLGIDCAGKPHEGLIIRNRASFPPSATIFTYHDHRMALAFAPLACIMDYLIILEPQV
                     INKSYPSFWEHLGQIGIQLEYFD"
     gene            complement(1356..2432)
                     /locus_tag="PK976_01930"
     CDS             complement(1356..2432)
                     /locus_tag="PK976_01930"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HRC66678.1"
                     /translation="MKGYTEIKSFEHPILLTGSRKILQDSITNLPFDRIMVLCDRNTW
                     RDCFHYIAPALCKKSCHIHIIKEGEDSKNLSTLEGIWNILSAEGFRRDDALLNLGGGV
                     VCDMGGLAAATFHRGMQFIHVPTSLLAMVDAAIGGKNGVNFRGLKNQIGVFAFPYITL
                     IYPVFLKTLPNRQIQSGFVEMLKHALLNGDDLWNRLSAIEHEEVMHHSHYISEALKVK
                     VSYVDKDPFDHGIRQALNLGHTAGHVFEEWSLLNQSEPMLHGEAIALGMLVELGLSVK
                     LTGLDENFALLASNVFRKHIHLPVPTLQDIGQLIDLAGFDKKNKDHFIRFSLLQKVGK
                     PVVQVAVSRETLSHAFRNTILSLT"
     gene            complement(2814..2945)
                     /locus_tag="PK976_01935"
     CDS             complement(2814..2945)
                     /locus_tag="PK976_01935"
                     /inference="COORDINATES: protein motif:HMM:NF016152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HRC66679.1"
                     /translation="MNKRHWNTVLVDGSIPDELLENWIDLSYDLVVSKLPVSLRNQL"
BASE COUNT          933 a          631 c          562 g          835 t
ORIGIN      
        1 tgcccgtgtc gggatagaat tgatcgataa aacaagggat gaaaatatcg acagtcattg
       61 caaagattaa tagcgcaaaa gtattaaaac tatgttattt ttcgacaaat gtcaatcaaa
      121 atattcgagt tgaatcccta tttgtccgag atgttcccag aaagatgggt aggatttatt
      181 gataacctga ggctccaaaa taataagata atccatgatg catgcaagag gagcaaaagc
      241 aagtgccatg cgatggtcat gataagtgaa gatagtagct gaaggaggga atgaagcacg
      301 attgcgaatt attaatcctt catggggttt acctgcacag tcaatgccaa gccttaaaag
      361 ttgttctttc aaagcctgat atcgatctgt ttccttgaat ctcaacgtgc tcaagttagc
      421 aaatacacca tcaatattta aagctgcagc ggttactatt actgataatg caatatccgg
      481 acaatttcga aaatcaaaat acatttcttt ggctactcct cctctccact tcagtagcaa
      541 tccctcgtgc gtagaggtag taacaacccc cattggttta aatgcttcta taagtagctt
      601 atcggcctga agagaattca tatcgagatt ttcgagtaaa aactcagagg atggctggag
      661 tgcacttatg gcaaaccatg gtgcagcaga gctccagtcg ggctccatag caatgcttcc
      721 tgaaaaattg taacccgaat tatctacctc aatcatgttg tttttcagtc gtcttgcttt
      781 cgccccgcat cttctcatac attgtatcgt caaatctaca taaggcatcg agacttcttg
      841 atttatcaga tgtatattca atggatcggg aagcataggc gcaatcatca gcaaagctga
      901 aacgaactga ctgctgatat cggcgtttat ctggatattc ccgccatgaa gctggccacc
      961 tttaaaatgc aaaggcagac gatcaggagt atccaaatat tcgatcatgg ctcccatcga
     1021 acgcaaagta ttcaccaaag ctgaaatagg acgccgaagc aagttgcccg aaccggttaa
     1081 aatgacgggt ttagccctca aggcaaaata agctgtcaaa aaacgagccg cagtgccagc
     1141 ctcagccaca ttaatcacat caaaaccctc ttcatatctt cgaatggctt ctgccatcac
     1201 ccgggtatcg tcagcatccg acaaatgcgc tatctcgaat gtatccccgg caagtgcctg
     1261 aattatcaac aacctgttgc tgatactttt cgaataaggc aattgaatgc tcacctttga
     1321 tggaatgggt cgataagtca gaattaaccg attcatcatg tcagagataa aatagtattt
     1381 ctgaaagcat ggctcaaagt ctcccgtgaa acagcaacct gaactacagg ctttcctact
     1441 ttctgcagca aagaaaatcg aatgaagtga tctttgtttt ttttgtcgaa accagccaaa
     1501 tcgataagtt gtccaatatc ttgcaaagtg gggacgggta aatgaatatg ttttctaaaa
     1561 acattagagg caagcaaagc aaaattctca tccaaaccag tcagttttac cgacaacccg
     1621 agttcgacga gcattcccaa agctatagcc tcgccatgga gcatgggttc ggattgattc
     1681 aacaacgacc actcctcgaa aacatggccg gcggtatgcc ctaaattgag agcttgcctt
     1741 attccatgat cgaaaggatc tttgtctacg taagagactt ttaccttcaa agcttcagaa
     1801 atgtaatgcg aatgatgcat aacctcttca tgttcgattg cgcttaaacg attccacaaa
     1861 tcatctccat ttagcaaggc atgtttcagc atctcaacga accccgattg tatctgccta
     1921 ttaggtaagg ttttcaaaaa tacaggataa atcaatgtta tataaggaaa tgcaaaaacg
     1981 cctatctgat tcttcagacc cctaaaattg actccatttt ttccgccaat ggccgcatcc
     2041 accatggcca ataaagaagt aggcacatga ataaattgca ttccccgatg aaatgtggca
     2101 gcagccaacc cccccatgtc acacaccaca ccacctccta aattcagcaa ggcatcgtcg
     2161 cgtctaaatc cttctgccga taaaatattc caaatgcctt ccaatgtgga aaggtttttc
     2221 gagtcctccc cttctttgat aatatgaata tgacaagatt ttttgcaaag tgcaggtgca
     2281 atgtaatgga aacaatctct ccatgtatta cggtcgcata aaaccatgat gcgatcgaat
     2341 ggcagattgg taattgaatc ttgtaagatt ttcctactac ccgtaagcag gatgggatgt
     2401 tcaaaggatt ttatttcagt ataacccttc ataagatgat ggcagtttaa aaccttgttt
     2461 cattatataa atcaaaatca ataacatatt tattttctac tttttgcaaa attttgtaat
     2521 taatttaatt tcagcaagat aaaaaggaaa atatattcac ttatttaaat ttatttattt
     2581 ttacaaaaat ttttctgtca aatatcatga agccaaagat aagaaggatt cgttttttca
     2641 agataatggg gaaagtcgtt ggcctggcaa tgggatatcc atattacaag caaataggct
     2701 gcaatgctgg tggatgccga atcacctcga attcttatct aagtgtatta tgggggatgt
     2761 ttatcggggt atttgattgt catcatattg cttatacctc ggaaatacag ccctcaaagc
     2821 tgatttcgta aagatacagg caatttcgat acgaccaaat catagctgag gtctatccaa
     2881 ttctctaata gctcatccgg aatgcttcca tcgacgagaa cagtattcca atgccgttta
     2941 ttcatgtgat atcctggcaa g
//
LOCUS       DAORPD010000109         2947 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_5977_length_2947_cov_2.709544, whole genome shotgun sequence.
ACCESSION   DAORPD010000109 DAORPD010000000
VERSION     DAORPD010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5977_length_2947_cov_2.709544"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /gene="sprA"
                     /locus_tag="PK976_01940"
     CDS             complement(<1..888)
                     /gene="sprA"
                     /locus_tag="PK976_01940"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HRC66680.1"
                     /translation="MKTSIIPFIRYFVALGFVVALSGFSAADPKASSTDYSLAFFAPP
                     DTIPLRYPFKDRDLYSPPASEHQLFLRNPSSIKVNVEYDGESNQYILTETMGSLQVRP
                     PLVMDFKEYMEYDASRALNSYWREKSLSTKGEKSDGIIPSIYIGGQAFDKIFGGSTID
                     VRPNGSAELIFGIRSNKNEDPSLAVRQRKTVNFDFQEKIQMNVIAKVGDKIEFKANYN
                     TESSFDFENKIQLKYEGKEDEILKLLEVGNVNLPLNSTLIQGSQSLFGVKTKMQFGRT
                     TITAVVSQQQSETKNITVEG"
     gene            complement(909..1493)
                     /gene="ruvA"
                     /locus_tag="PK976_01945"
     CDS             complement(909..1493)
                     /gene="ruvA"
                     /locus_tag="PK976_01945"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007217309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HRC66681.1"
                     /translation="MYAYLEGQIAELNPAYAVIDCNGVGYEVNISLNTFSSIKDKERV
                     RLYTHLVVRDDAQMLYGFAEESERTLFRHLITVTGIGPSTARMILSSMTPVELISAIA
                     SGNEEILKRVKGIGTKTAQRILIELKDKVGKVTTSQGSLPVLGNRNLEEALSGLVILG
                     FSKTLVQRALEKIVSTRGDSISVEEMIKEALKVL"
     gene            1679..>2947
                     /locus_tag="PK976_01950"
     CDS             1679..>2947
                     /locus_tag="PK976_01950"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66682.1"
                     /translation="MKKFCFFLIVSLYAPIASSQPALVNEWWDQAKGINKQEGQILDT
                     LRIGHWITWYPDGTKESEGNYHDGNKAGKWEYWFPDGKLNSILNIENGPCILYHPNGK
                     ICMEWQVIHGKRNGKLEEYDTAGHLIRQITYQNGLKQGFVQTFYPTGQKSFEGIYRND
                     SLNGKAIWYYPQGNKEMEGNLHNDLQEGWWIFYHPNGTKGSEGKFKEGKRTGKWVFYY
                     ETGEKWREGEYADDQRQGHWITFYETGQRFREGNFVNDKEQGLWVEYYQNENINYQGY
                     FEGGLMEGLWEAWYEDGKTRYRNHYSLGIPHGKWTYFYRNGNKKYEGYYVNGRKNGLW
                     TEYYQDGSMESKANYTDGRRQGTAFFYEPNGLLIRQQDFLNDEPHGSFISFYPNGKKS
                     SEGVHYRGSPTGIWKFYSHDGKLIMTKTYSN"
BASE COUNT          925 a          588 c          608 g          826 t
ORIGIN      
        1 accttcaacg gtaatatttt tcgtttcgct ttgctgctgc gaaaccaccg cagtaatggt
       61 agtacgtcca aattgcatct tggtttttac accgaacaag ctctgactgc cctgaataag
      121 cgtagaattc aaaggaaggt ttacgttgcc aacttctaaa agtttcaaaa tttcatcctc
      181 tttcccttcg tatttgagct ggattttgtt ctcgaaatca aaactcgatt cggtattgta
      241 attggctttg aactctatct tatcgcccac tttggcaatg acattcattt ggattttttc
      301 ctgaaagtcg aagtttacgg tttttctttg tctgactgca aggctaggat cttcattttt
      361 attggatcga atgccaaaaa tcagttcggc cgaaccattg ggacgaacat ctatggtgct
      421 tccgccaaag atcttatcga aagcctgtcc tccaatataa atggaaggta taataccatc
      481 gctcttttct cccttagtac tcaaagattt ctcacgccag taactgttca gagctcgcga
      541 tgcatcatat tccatatatt ctttgaaatc catcaccaat ggaggcctta cctgaagcga
      601 acccatagtt tcagttagga tatattgatt cgattctccg tcatattcta cattcacctt
      661 tatgctcgag gggtttcgca gaaacaattg gtgttcggag gctgggggcg aataaaggtc
      721 cctgtccttg aaaggatagc gtaaaggaat agtatcggga ggcgcaaaaa atgcaagtga
      781 ataatccgta gaactggctt tcggatcggc tgcggaaaaa cccgaaagag ccaccacaaa
      841 gccaagagca acaaaataac ggataaatgg tataatagac gttttcaagc caactgatag
      901 ttttgttttt acaatacctt tagtgcttcc ttgatcatct cctccaccga gatagaatct
      961 ccccgtgtgg aaacgatctt ttccaaagct cgttgaacta aggttttact aaagcccaaa
     1021 atgaccaaac cagataacgc ttcttctaaa tttctattgc ctaaaacagg taaacttccc
     1081 tgagaagttg ttaccttccc tactttatcc tttaattcga tgagaatacg ctgagcagtt
     1141 ttagttccaa tgccctttac tcgtttcaga atctcttcgt ttccgcttgc aatggccgaa
     1201 atcagttcaa caggagtcat cgaggataga atcattcggg cagtactagg gcctatgccc
     1261 gtcaccgtga tcaaatgcct gaataaagta cgttcgcttt cctccgcaaa accataaagc
     1321 atctgtgcat catctcgcac aaccagatga gtataaagcc gaaccctttc tttatccttt
     1381 atactcgaaa acgtattcaa cgaaatgttt acttcgtaac ccactccatt acagtcaatt
     1441 actgcataag caggattcaa ttcggctatc tgtccctcaa ggtaagcgta cataaattta
     1501 gcagaaatgg ctggtttagt taataaaagg aagaattcga acttccttgg ctagcaaaat
     1561 tacaattttc cgatttggga ataaaatcat catccctgcc aaaatttgat cttatcctaa
     1621 atcaattaca atttcgagct aattttgcag cttgtggttt tttgaattta ccaaaccaat
     1681 gaaaaaattc tgcttctttc ttatagtttc tttatatgct cctatagctt catctcaacc
     1741 ggcgctggta aatgaatggt gggaccaggc aaaaggcatt aataaacagg aaggacaaat
     1801 cctcgacact ttaagaatag gacattggat cacatggtat cccgatggaa ccaaagaatc
     1861 ggaaggcaat tatcatgatg ggaacaaagc tggcaaatgg gaatattggt ttcccgatgg
     1921 aaaattaaac tcaatactga acatcgaaaa tggtccttgc atcttatatc atccaaatgg
     1981 taaaatttgc atggagtggc aagtcattca tggcaagcgt aatggaaagc tcgaagaata
     2041 tgatactgct ggacatctga ttcgtcaaat cacttaccag aacggactaa aacaagggtt
     2101 tgtgcagacg ttttacccga cagggcaaaa atcattcgaa ggcatataca gaaacgatag
     2161 cctcaacggg aaagccattt ggtattatcc gcagggaaat aaagaaatgg aagggaattt
     2221 acataacgac cttcaggaag gatggtggat tttttatcat cctaacggaa cgaaaggtag
     2281 tgaaggaaaa tttaaagaag ggaaaagaac agggaaatgg gtgttttatt acgaaacagg
     2341 agagaaatgg cgcgaaggtg aatatgcaga cgatcaacgt cagggtcatt ggataacatt
     2401 ttatgaaact ggccaaaggt ttcgagaagg caacttcgtc aatgacaagg aacaggggtt
     2461 gtgggtcgag tattatcaaa atgagaatat caactatcag ggttatttcg agggaggatt
     2521 aatggaaggt ttatgggaag catggtatga ggatgggaaa actcgctatc gaaatcacta
     2581 ttcacttggt atcccccatg gtaaatggac ttatttctat cgcaacggaa ataagaaata
     2641 cgaaggatat tacgtgaatg gcaggaaaaa tggtctttgg acagaatact atcaagatgg
     2701 atcgatggaa agcaaagcca attatacaga tgggcgtaga caaggaactg ccttctttta
     2761 tgagcccaat ggcttattga ttcgtcagca agattttcta aatgacgaac ctcatggtag
     2821 tttcatatct ttttatccca acggtaagaa aagcagcgaa ggcgttcact atcgaggcag
     2881 ccccactggt atctggaaat tttacagcca tgatgggaaa ttgataatga ctaaaacata
     2941 cagtaac
//
LOCUS       DAORPD010000110         2944 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_5987_length_2944_cov_2.289373, whole genome shotgun sequence.
ACCESSION   DAORPD010000110 DAORPD010000000
VERSION     DAORPD010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5987_length_2944_cov_2.289373"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /gene="meaB"
                     /locus_tag="PK976_01955"
     CDS             complement(<1..774)
                     /gene="meaB"
                     /locus_tag="PK976_01955"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HRC66683.1"
                     /translation="MDSKKKKCVNTLKVNEGIEPPPSINEELLKKPLKRKDIHPAEYY
                     VEGIFGGNRSILSQAITLVESSLKTHQKLAQKIIECCLKKAGNSVRIGITGVPGVGKS
                     TFIEAFGKQLTQTGHKVAVLAIDPSSQRSKGSILGDKTRMMKLSADPNAYIRPSPSAG
                     SLGGVARKTRGTIILCEAAGFDVIIIETVGVGQSETAVHSMVDFFLLLMLAGAGDELQ
                     GIKRGIMEMADAIVINKADGDNLLKAEQAKSQYESALHLF"
     gene            complement(1265..>2944)
                     /locus_tag="PK976_01960"
     CDS             complement(1265..>2944)
                     /locus_tag="PK976_01960"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC66684.1"
                     /translation="GSSSSKNLGTTPNGGRDAWLLKTDYLGNILSTQGFGGSGFEYFN
                     NVFPAPDSGLYLLGSTISSDDIFSFNPYLGGISAFALKLDSSNNIVWNHIYGGNRTDE
                     LKDAVMTYDGGFVFVVWSTSNDGDVGQNFGAVDVWVVKLTDEGQILWSKVFGNHFIDI
                     VSTIIETSDKGLLIGGSFDYYKPGLGNLFCDTCYGNAEAFLIKLDSVGNVCWTKCYGG
                     PGYDGFSSLLEVSDGYVLGGYASAGGGLVTGFHNNAMGYNDAWVIKTDFEGNIIWTKC
                     LGGSGTEIVYKMFKEKDGNLMIFSMTDSHDGDVNSNFSDYYYMWLVLLNGQDGSIIKE
                     KCINVVGTYWGAAAQIEYGDYILLINVPTLYNWVDVWFYRIKDCNEEQIPPAPAEPKG
                     PQQINTYTTTTSFYSLTPDGIALSYTWELNPPEAGWLMTPADTTIEVVWNPNFWGTAR
                     LKIRGTYLCGIGPWSSELKISVNVVGMEEPDKEGFCVWPNPSNDRFIFELPASASYTI
                     QITDISGRQIEKIETAGGTTQWDASACEPGIYLYRITSEGFLKTGKLVKQK"
BASE COUNT          866 a          675 c          549 g          854 t
ORIGIN      
        1 gaaaagatgc agtgcacttt cgtattgact tttagcctgt tcggctttca ggagattatc
       61 tccatctgcc ttgttgataa cgattgcatc ggccatttcc attattcctc ttttgatgcc
      121 ttggagctca tcgccagccc cggccagcat caataacaga aaaaaatcga ccatagaatg
      181 aacggcagtt tccgattgcc ctacaccaac ggtttcgatg atgataacat caaatcctgc
      241 tgcttcgcat aaaataatcg ttccacgagt tttacgggca acacctccaa gcgaacctgc
      301 cgaaggagaa ggcctgatat aagcattggg atcagccgat aacttcatca ttcgggtctt
      361 atcgcccaag atacttcctt tcgagcgttg actgctggga tcgatggcca aaacggcaac
      421 cttatgccct gtttgggtaa gctgtttccc aaatgcctcg atgaaagtac tcttgcccac
      481 tcccggcacg cccgtaatcc caattctcac cgagtttcct gccttcttca aacaacactc
      541 gataatcttt tgcgcaagtt tctgatgagt ctttagtgaa ctttcgacta aagtaatggc
      601 ttgagagaga atagaacggt tccccccaaa aattccttcc acataatatt cggcaggatg
      661 gatatccttt cgtttcaacg gctttttcaa taattcttcg ttgatggaag gaggaggctc
      721 gataccttca ttcactttca aggtattaac gcactttttc tttttactat ccattcattg
      781 gaatttgttg tgcaaaatta aacttgcttt tcattaagtt tttgctaaga taaatcatct
      841 ttagaaagat tctgtaaatt gaaattaatt ttccccagtt tttcgggaat gtcccttatt
      901 caaaaagatt gactattatt gagtaaatta ttagtggatg gtgattagtg gatggtggat
      961 ggataagtta aaagttcata aagtttaaaa cggaaaacgg aagaagggag acgtggggac
     1021 gaggagagga ggggacgagg agactgggag acgaggggac aaggggactg ttagatagtg
     1081 gatggtgatt agtgtgtgtg tcgatgtcta aggatatcgc agaaaaaagc tttcgcagga
     1141 gcacacagaa aaatatcagc gtaaatcagc ggattaaatc tgcgtcaatc agcgagaaaa
     1201 aagttatccg actttggcgg ttcccaagtc tccctgtctt gcggtcttct tatgctattt
     1261 cgctttattt ctgttttact aacttaccgg tttttaaaaa accttcggaa gtgatgcgat
     1321 aaagataaat ccctggttcg caggccgaag catcccattg cgtagttcct ccggcagttt
     1381 ctattttttc tatctgtctg cccgaaatgt cggtgatttg tattgtatat gaggctgaag
     1441 ccggtagctc gaaaatgaat ctatcattgg aaggattggg ccatacacaa aatccttcct
     1501 tatctggttc ttccatgcct accacattta ccgaaatctt cagctccgac gaccaaggcc
     1561 ctataccaca aagataagtg ccccttatct tcaaacgtgc agttccccag aaatttggat
     1621 tccatactac ttctatcgta gtatctgctg gcgtcatcaa ccagccagct tctggtggat
     1681 ttaattccca ggtgtacgac aatgcaattc catcgggtgt gagcgaataa aaggaagtgg
     1741 tagtagtata ggtattgatt tgctgtggcc cttttggctc tgcaggcgct ggtggtatct
     1801 gttcttcatt acaatccttg atacggtaaa accaaacgtc aacccaatta taaagtgttg
     1861 gaacattaat tagtaatata taatccccat attcaatttg agcagccgct ccccaataag
     1921 tacctactac attaatacat ttttccttga tgatactccc atcctgacca ttgagcaata
     1981 ccagccacat ataataataa tcactaaaat ttgaatttac gtctccatca tgagaatcag
     2041 tcatagagaa aatcatcaga ttgccatctt tttctttgaa cattttataa actatttctg
     2101 tgcctgatcc tccaagacat ttcgtccaga tgatgtttcc ctcgaaatcc gtctttataa
     2161 cccaggcatc attataaccc atagcgttat tgtgaaaacc agtaaccaat ccaccacctg
     2221 cgcttgcata acctcccaat acatatccat ctgacacttc gagtaaacta gaaaatccat
     2281 catacccagg cccaccgtaa catttcgtcc agcatacgtt cccaaccgaa tccagcttga
     2341 taagaaatgc ttcggcattc ccataacagg tatcacagaa cagattgccc agaccaggct
     2401 tataataatc gaaagaaccc cctatcaata gacctttatc agaggtctcg attatggtag
     2461 aaacgatatc gataaaatga ttgccaaaga cctttgacca taatatttgg ccctcatcgg
     2521 taagttttac aacccacaca tcgacggcac caaaattctg acccacatct ccatcattag
     2581 aagttgacca taccacaaag acaaagcctc catcataagt cattactgca tcctttaatt
     2641 catcagttct attccctcca taaatatgat tccatacaat attattgctg ctgtcgagtt
     2701 ttaatgcaaa tgcagaaatg ccacctaaat atggattgaa actaaatata tcatcactgg
     2761 aaatggtact gcccagtaaa tataaaccag aatcgggagc tgggaaaaca ttgttgaaat
     2821 attcaaatcc actacctcca aacccctggg tcgataatat attccccaaa tagtcggtct
     2881 taagtaacca agcatctctc cctccattcg gtgtagtacc taaattctta gaagaagaag
     2941 atcc
//
LOCUS       DAORPD010000111         2937 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_6009_length_2937_cov_2.992366, whole genome shotgun sequence.
ACCESSION   DAORPD010000111 DAORPD010000000
VERSION     DAORPD010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6009_length_2937_cov_2.992366"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="PK976_01965"
     CDS             <1..308
                     /locus_tag="PK976_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRC66685.1"
                     /translation="VTALHGGYPYLAPTNIPAYKAAQKAHLKTFGKYPIPTCSGGSIP
                     IISTFDRVLGIKSLLLGFGLESDAIHSPNENFSVKNFFKGIETISEFYIQFAHITKD"
     gene            312..773
                     /locus_tag="PK976_01970"
     CDS             312..773
                     /locus_tag="PK976_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66686.1"
                     /translation="MESDDNFEDKIEKLIRLFKKLKEQAKPETIAGFDPFMMSQLDFL
                     ISNFERMKYDPQARSIFQQMGKPFENMLNLFIKNLSEQLGEPVNIPKKSERETLLRPL
                     PEEEKPSIGTLTRRLVQIDKLLAEGNLNQEEEDQLLDERIKIMKKLNPEDE"
     gene            800..1378
                     /gene="thpR"
                     /locus_tag="PK976_01975"
     CDS             800..1378
                     /gene="thpR"
                     /locus_tag="PK976_01975"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HRC66687.1"
                     /translation="MTLKRIFIAVDYQPDSDFLYYFSFLRRQLRNEDITWSATQNFHI
                     TLKFFGDTPEDHISSIIHQLDGITKKYESFQIQPNKLGIFGSLKQARALWLSINESTG
                     TLESLAQEIEHAFLTDPSKKNNSFTPHLTLGRPKFIRQPDLLKNLMESSSQHVFNPFA
                     IREVLLIWSTLTPLGPQYKTLKSFPLQPLTLE"
     gene            complement(1394..1729)
                     /locus_tag="PK976_01980"
     CDS             complement(1394..1729)
                     /locus_tag="PK976_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66688.1"
                     /translation="MNGEILIPLGFFAMVVAIVYLALRQRERMKMINQGMDPAASNTG
                     LESTLQLRLGLIFIGIAVGFLLGDIFSSLHWLKEWVAYISMVTIFTGIALIVSYYLIN
                     KIPPRDRKE"
     gene            1890..2483
                     /locus_tag="PK976_01985"
     CDS             1890..2483
                     /locus_tag="PK976_01985"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRC66689.1"
                     /translation="MAEINDEILIQRTLRGDQFAFAQIVEKYKQTAFSIAYQITGNRE
                     DAEEIAMDAFVKAYQALKGFKFESKFSTWLYRIVYNTAISKKRKKKMEFVAIDHHLIE
                     NYSEDNFVAGLQMLDTDEQLKLVQHVLKSLPGDDAALINLFYNQQLNIEEISHITSLS
                     VSNVKVKLHRIRKRIFAGVQLLLNQSTSTYEYKNSKS"
     gene            2480..2923
                     /locus_tag="PK976_01990"
     CDS             2480..2923
                     /locus_tag="PK976_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66690.1"
                     /translation="MNIQTNKSDDLLREVFSKIPSESPSEDFTQKVMDQIARENVEKL
                     SINANFNLGFRTLLALALTIGFFGILIFTSDFSFMDYIYQIFSTLKFKLNPNQLFHPF
                     VFLSKTNNLSLIVGLVMLAGGALLVFSHNTNKKDKSPGMDSFLSL"
BASE COUNT          954 a          660 c          506 g          817 t
ORIGIN      
        1 aagtaaccgc attgcacgga ggctatccat atttagcacc caccaacatt ccagcgtata
       61 aggcagctca aaaagcccat ttgaaaacct ttggcaaata tcccattcct acctgcagtg
      121 gaggctctat tcccatcatc agtaccttcg atagagtatt gggaataaaa tcactacttt
      181 taggcttcgg cctcgaatct gacgctattc attcacccaa cgaaaacttc tccgtaaaga
      241 acttcttcaa gggaatagaa accatctctg agttctatat tcaatttgct cacatcacga
      301 aagattaata catggaatca gacgataatt tcgaagataa aatcgaaaaa ctcatccgct
      361 tgttcaagaa gctgaaggaa caagcaaagc cggaaacgat tgcgggcttc gatcccttca
      421 tgatgagcca gctcgatttc cttatcagca attttgagag aatgaaatac gatccccagg
      481 cgcgtagtat atttcaacaa atgggtaaac cttttgaaaa tatgttgaat ctttttatta
      541 aaaatctttc cgaacaactt ggcgaacctg ttaatatacc caaaaaatca gaaagggaaa
      601 ccttacttcg acccctgccc gaagaagaaa aaccttctat tggcacgctt acacggcgcc
      661 tggtacaaat cgataaacta ttggccgaag gtaatctaaa tcaggaagaa gaagatcagc
      721 tactggacga aagaatcaag atcatgaaaa aactgaatcc agaagatgaa taaatgcaat
      781 atttacattt ggcactaaaa tgacactaaa acgtattttt atagccgtcg attaccaacc
      841 cgattcggat ttcctctatt atttttcatt cttgcgccgt cagctaagaa atgaagacat
      901 cacatggtcg gccactcaaa attttcacat tacactgaaa ttttttggag atactcctga
      961 agaccacata tcttccatta tccatcagtt agatggaatc actaaaaaat atgaatcgtt
     1021 tcagattcag cccaataaat tgggaatttt tggcagccta aaacaagctc gagcattatg
     1081 gttaagcatc aatgaatcca ctggaactct cgaaagttta gctcaagaga tcgagcatgc
     1141 cttcctcact gacccatcaa aaaagaataa ttcatttaca ccacacctca ccctgggacg
     1201 tcctaaattc attcgccagc ccgatttgct gaaaaaccta atggaatcat catctcaaca
     1261 tgttttcaat ccttttgcca ttcgtgaagt attgcttatt tggagcacac tcacgccttt
     1321 aggacctcag tataaaaccc tgaaaagttt ccctttacag ccgttaacac tcgaataatc
     1381 ctcaaatgga tcattattct ttcctatcgc gtggaggaat tttgttaatc aggtaataac
     1441 tcacgatgag ggcaatgccc gtgaaaatag taaccattga tatgtacgct acccattcct
     1501 taagccagtg aagcgaactg aagatatctc ccagcaaaaa accaacagca attccaatga
     1561 aaatgagccc cagacgaagt tgaagcgtgc tctccaagcc cgtattgctt gccgctggat
     1621 ccataccctg attgatcatc ttcatacgct ctctttgcct taatgccaga taaacaatgg
     1681 ctactaccat tgcaaaaaaa cctaagggaa ttaaaatctc gccattcata tcacgataat
     1741 attaaaatta acaatgtcag catttgttgt ccacgtgttt ttgacgcaac aaatccaaac
     1801 cggttacacc cctgagacga aaatgttgaa tccctaaata tttactgcta agtttgtaac
     1861 caacccgaaa tgaaggcgtc aaactcccaa tggcagaaat caacgacgaa atattaattc
     1921 aacgtactct tcgaggtgat caatttgcat ttgcccaaat agtcgaaaaa tacaaacaaa
     1981 cggcttttag cattgcgtat cagattacgg gtaaccgcga agatgccgaa gaaatagcta
     2041 tggatgcctt cgttaaggcc tatcaggcgc tcaagggatt caaattcgaa tcgaaattca
     2101 gtacttggct ttatcgaatt gtttacaata cggctatctc gaaaaaaaga aagaaaaaaa
     2161 tggaatttgt agccatcgac catcatctga tagaaaatta ttctgaagac aattttgtag
     2221 ccggtttgca aatgctcgat accgatgaac agcttaaatt ggtccagcac gttctgaaat
     2281 cattaccagg cgacgacgct gctttgataa atttgtttta taatcaacaa ttgaatatcg
     2341 aagaaattag ccatatcacc tcgctctccg tctctaacgt taaagtaaaa cttcaccgaa
     2401 tacgaaaacg tatttttgcg ggtgttcagc ttcttctcaa tcagtccacc tctacctatg
     2461 aatataaaaa ctcaaaatca tgaatataca aaccaataag tccgacgacc tgctcaggga
     2521 ggtgttcagt aaaatccctt ccgagtctcc ttccgaagat tttacacaga aggtaatgga
     2581 tcagattgcc agagagaatg tcgaaaaact atctatcaat gcaaatttca atttgggatt
     2641 tcgcaccttg cttgctttgg ctttaaccat tggatttttc ggaatcctca tctttacgag
     2701 cgattttagt ttcatggatt acatttacca gatattttcg accttgaaat ttaaacttaa
     2761 ccccaatcaa ttgttccatc cttttgtatt tttatcgaaa acgaataatc tgtcgcttat
     2821 cgttggcctt gtgatgcttg ctggtggcgc actcttggtg ttttcgcaca atacgaataa
     2881 aaaggacaaa tcgccgggta tggattcttt cctgagctta taagcctttg cactgcc
//
LOCUS       DAORPD010000112         2912 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_6072_length_2912_cov_2.241512, whole genome shotgun sequence.
ACCESSION   DAORPD010000112 DAORPD010000000
VERSION     DAORPD010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6072_length_2912_cov_2.241512"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1398
                     /locus_tag="PK976_01995"
     CDS             <1..1398
                     /locus_tag="PK976_01995"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HRC66691.1"
                     /translation="GGGSSHVVPWRAIDPLTQLQNVAGNQIEIHYAQGVKLGASPLQA
                     VPSEFLITKDGKHGIIGEYFKGIELQGSPAFRRIDTNIDFIWEENEPVPSLGKDHFSV
                     RWTGYLLAPKSRNYVLYTASDDGIRLYVNNKLMIDNWTDHGTTIDSARIFMKAGEKYE
                     IRLEFFENAGSAICQLGWDYFGAEGETGKMIDEAVQAAKKSDLAVVFTGSSEFIESEG
                     YDRIGGMKLSAGQDELIDAVVKVNPQTIVVSYGGTSIIMSKWGEKVPAILDVFFPGQE
                     GSRAIIDILLGKANPSGKLPFTFIQSPQQSPAYKNYQDPSKIAHYSEGIYAGYRYYEK
                     FGIQPAFPFGFGLSYTEFEYSNLQVEPLSKDSCLIRLDIKNTGEMAGTEIVQIYVVPP
                     DASNRPVKELKGFAAVTLQPNESQNVKILLNPRAFSYYDQTQGKWVIQSGKYKILTAS
                     SSTDIRQQASFEIMP"
     gene            complement(1490..>2912)
                     /locus_tag="PK976_02000"
     CDS             complement(1490..>2912)
                     /locus_tag="PK976_02000"
                     /inference="COORDINATES: protein motif:HMM:NF014147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HRC66692.1"
                     /translation="LSALGLFSACGKNDNPQAEPPVDTTHYAKAQVWLTKGDKTVLFS
                     KQNGLNILTSVAPKWPTITVDTSFRFQEIEGYGAALTGSSAYLFNRVLTPNARQSLIE
                     NLFTPDSGIGLSYLRLTIGASDFSLSDFTYDDVEPGQTDFNLQNFSLSQDTLDVVPIL
                     KEILKVAPNIRLLGSPWSPPAWMKTNGSLKGGRLKQECYPVYARYFVKYIQSMQTNGI
                     RIDAITPQNEPLYATASYPCMLMEAAHQKELIRDHLGPAFQQYGLDVKIIVYDHNWDR
                     PDYPIEILNDQETRKYVAGSAFHAYAGDVSTMSFVHQAHPDKAIYFTEISGGDWATNF
                     SDNLLWNMKNILIGTANNWSKNALFWNLALDENHGPKNNGCSNCRGVVTITSQGQIYK
                     NEEYYSLAHFSKFIRPEAVRILSRIAQQLMNIDAVAFQNIDSSKVLIVANYSDTYKTF
                     TVIQNQKSFNYSIAPKSVATLIW"
BASE COUNT          855 a          566 c          658 g          833 t
ORIGIN      
        1 ggaggcggga gctcgcatgt cgttccttgg cgagccatcg atcctctaac tcaactccaa
       61 aatgttgccg gcaaccagat agaaattcac tacgcacagg gagtaaaact cggtgcaagt
      121 cctttgcagg cagtcccttc agaatttctt ataacaaagg atggtaaaca cggtattatt
      181 ggagaatact tcaaaggaat cgaactgcaa ggttctcctg catttcgacg tatagatacc
      241 aatattgatt ttatttggga agagaacgag ccagttcctt cattgggcaa agaccatttt
      301 tcggtacgat ggacggggta tcttttggct cccaaaagcc ggaattacgt tttatatact
      361 gccagcgacg atggcatcag gctttatgta aataacaagt tgatgataga caattggact
      421 gaccatggta caaccatcga ctcggctcgg atttttatga aagctggtga aaaatatgag
      481 atcaggcttg aattttttga aaacgctggc tctgcaatat gtcagttggg ttgggattac
      541 tttggtgctg agggtgaaac gggtaaaatg atagatgaag ccgttcaggc tgctaagaag
      601 agcgatttag ctgtagtttt taccggttca agtgaattta ttgaaagtga aggatacgac
      661 aggattggag gtatgaagct atccgccggc caggatgaac tcatcgatgc tgtggtgaag
      721 gttaatccac aaaccatagt ggtttcatac ggaggaacga gcataatcat gagtaaatgg
      781 ggagaaaaag ttccagccat cttggatgta ttcttccctg ggcaagaagg ttcgagagca
      841 attattgata tattgttggg taaagcgaat ccttcgggaa aattaccttt cactttcatt
      901 cagtcgcctc agcaatcgcc tgcttataaa aactatcagg atccttctaa gattgctcat
      961 tacagtgaag gcatttatgc gggttacaga tattatgaga aatttggaat ccagcctgca
     1021 tttcctttcg gattcggatt atcctatacc gaattcgaat acagcaactt gcaggtggaa
     1081 cccctatcca aagattcgtg ccttatccgt ctcgatataa aaaatactgg agagatggca
     1141 ggaactgaaa tcgttcagat ttatgttgtt cctcccgatg ccagcaacag accagtgaaa
     1201 gagcttaaag gatttgcggc tgttacgctt caacccaacg aaagccaaaa cgtaaagatt
     1261 cttctcaatc cacgtgcctt ctcttattat gatcaaaccc aaggaaagtg ggtcatacag
     1321 agtggaaaat acaagattct aactgctagt tcctcgactg atatccgtca gcaggcttct
     1381 ttcgaaatta tgccctgatt ctttgaaaat tgaaaaaatt atccagcttc caatgaacga
     1441 aagctggatt tttttatata caaagaatag tgtgaggata gttctgaaat taccagatga
     1501 gagttgctac cgattttggt gctatggaat agttgaaaga tttttgattt tgaataacag
     1561 taaatgtctt gtaggtatca ctataatttg caacgatgag cactttcgat gaatctatat
     1621 tttgaaaagc caccgcatca atattcatca attgctgagc gatgcgcgaa agaatgcgaa
     1681 ctgcctctgg gcgtatgaat ttggagaaat gtgccagact gtaatattct tcatttttat
     1741 aaatctgccc ttgagaagta attgtaacaa cgcctctgca gtttgagcat ccattgtttt
     1801 ttggcccatg gttttcatcc agagccagat tccaaaacaa ggcatttttc gaccagttgt
     1861 ttgcggtgcc aatgagtata ttcttcatat tccagagcaa gttgtcgcta aaattggtag
     1921 cccagtctcc acctgaaatt tcggtgaaat agatggcctt atcgggatgg gcttgatgga
     1981 cgaaactcat ggttgaaaca tcgccagcat aagcatgaaa agcgctgcca gcaacatatt
     2041 tgcgtgtttc ctgatcattg agaatctcaa tgggataatc gggtctatcc caattatggt
     2101 cataaacaat aatttttaca tccaatccgt attgttggaa tgctggcccc aaatgatcgc
     2161 gaatgagctc tttctggtgg gcagcttcca taagcatgca aggataagag gctgtggcat
     2221 ataaaggttc attctgggga gtaatggcgt ctatgcgtat cccattggtt tgcatggatt
     2281 gtatatattt tacgaaatat cgtgcataaa cggggtagca ttcctgcttt aagcgtccac
     2341 cctttaaact tccattggtt ttcatccaag ccggagggct ccaaggcgaa cccaataatc
     2401 ggatgtttgg agcaaccttc agaatttcct tcaatatagg aactacatca agagtatctt
     2461 gcgaaagaga gaagttttgc aaattaaaat ccgtttgtcc gggttcaaca tcatcataag
     2521 taaaatcgga aagcgaaaaa tcggatgctc ctattgtcag tctcagatag gataagccaa
     2581 tacctgaatc gggagtgaac aaattttcga tcaaactttg acgagcattc ggggtgagga
     2641 ctctgttaaa tagataagcc gatgaacccg tcagagctgc cccatagcct tcgatttctt
     2701 gaaatcgaaa agaagtatca acggtgattg taggccactt gggtgcaacc gaggtgagta
     2761 tattcaaacc attttgtttc gaaaataaaa cggttttatc cccttttgta agccatacct
     2821 gagcttttgc atagtgtgta gtatcaaccg gaggttcagc ttgtgggttg tcattttttc
     2881 cgcaagcaga aaaaaggcca agtgctgata gc
//
LOCUS       DAORPD010000113         2909 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_6082_length_2909_cov_1.969516, whole genome shotgun sequence.
ACCESSION   DAORPD010000113 DAORPD010000000
VERSION     DAORPD010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6082_length_2909_cov_1.969516"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="PK976_02005"
     CDS             <1..205
                     /locus_tag="PK976_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66693.1"
                     /translation="FEMAREMRKDPQLKDIPIIMLTSVDSVMGLNLKEMANDPKWLPV
                     DSYLEKPVQPQKLLDEIKRLLKL"
     gene            793..1941
                     /locus_tag="PK976_02010"
     CDS             793..1941
                     /locus_tag="PK976_02010"
                     /inference="COORDINATES: protein motif:HMM:NF027441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4831 family protein"
                     /protein_id="HRC66694.1"
                     /translation="MKYKFWFVIICCSFFVFQGLAQTNISLVSGKNVKPEKNGVFFSL
                     PRKIIKVSVSVREIENLRGPYAEYAQQLMGISNPVTQNTKEYEIADISMEGLVEPDPE
                     RIYFLEVDDKLSKEEKNFSVCLTSNGIIRSINGISSSKEAQLIKEEKKVIGQEDLFSM
                     SMSPSLYRKVDTIIRMVTVDTNTVKKRFFNTYFEEKPTEIKAREAADMVSRIRESRFN
                     LLTGYQETSYSPETMKYMDEKLQLMAQEYISLFKGLKTENLHTYTYYIVPDQNKSNQV
                     ICKFSKETGISDATDSKGKEIVLSIKRSGTMIQMPAWASAPVEVKSLLVPYRIPEMVR
                     LSVNYVGKVYDEQVIALPQFGVVSVVPFVKSKIEFDPQTGCIVKIYYE"
     gene            complement(1932..2759)
                     /gene="kdsA"
                     /locus_tag="PK976_02015"
     CDS             complement(1932..2759)
                     /gene="kdsA"
                     /locus_tag="PK976_02015"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HRC66695.1"
                     /translation="MIPDGLPLLRHKNSDNFFLIAGPCIVENPQMPLEIASVIYDICE
                     RLEIPFIFKASYKKANRTSIHSFTGIGDILALKAIADVRNELRIPVLTDFHTAEEAEL
                     AAAYVDVLQVPAFLCRQTELLVAAALTQKVINVKKGQFISPEAMQFVVEKLKSQGNNR
                     IILTERGTTFGYNDLIVDFRSIPIMQSFDVPVVVDITHSLQQPNTSAGVSGGSPQFIS
                     TIGKAAVAASADGIFIETHPEPSRALSDGANMLQLSLLENLLKQLVQIRKALNRNYS"
BASE COUNT          915 a          506 c          608 g          880 t
ORIGIN      
        1 gtttgaaatg gcacgcgaaa tgcgtaaaga tccccagctg aaagatattc ccatcatcat
       61 gttgacaagt gtggattcag ttatgggtct caacttgaaa gaaatggcca atgatcccaa
      121 atggcttccc gtggattcct acctcgaaaa acctgtccaa ccgcaaaaac tcctggacga
      181 aataaaacgc ttgctcaagc tttgaaaagc ttatatttca aattcaatag ccggtctgct
      241 taggccggct attgaatgtt acgcgcatac tcagattgtc cctttctcac tacgttgcct
      301 tagattatga tcttttgctc agaatagcaa gcatgcacgt tgaccaagct ggatataata
      361 aagcaatcaa ctgagccgct ttcactatgt atagctccgt gtatatcttt ggaaaccttt
      421 gttttatgta aaatcgaaga ataggattat atcaaaacgg gatctgattc aaacggattt
      481 atcatatagt tttactattt ctattatttt gagaataatg attctatttc tgaagtggag
      541 aatcaaaata aattttatcc gatgattgga ataaaaataa ggaatgtatg tatttaaacg
      601 ctctgtttct tatttaatac atgagtttgg aagtgtatcc gggtaatgtc cgacttatat
      661 tgaatagcta aatgtgaata tttaaaatta tcgaacaaat acaacaaatc aacgaaagaa
      721 ttaattttgt gaaaacttct cgtagacgaa gaagaaaatt tgttgaaatt tattccaaaa
      781 aaattgatcg atatgaaata taaattttgg tttgttataa tttgctgttc ttttttcgtt
      841 tttcagggtt tggcacagac caacatcagt ttggtgagcg gtaaaaatgt gaagcccgag
      901 aaaaatggag tttttttttc cttaccacgg aaaatcatca aggtttcggt aagtgttaga
      961 gagatagaaa atttgagagg gccttatgcc gaatatgccc agcagctcat gggcataagc
     1021 aatcctgtta ctcaaaatac aaaggaatat gaaattgccg atataagtat ggagggcctt
     1081 gtagaacctg atcccgaaag gatttacttc cttgaggtgg atgataaatt gagcaaagag
     1141 gagaagaatt tttcggtatg tttgacttct aacggaatca ttcgtagtat taatggaatt
     1201 tcttcgtcta aagaagctca actcatcaaa gaagaaaaaa aagtcattgg tcaggaagat
     1261 ttattttcca tgagtatgag tccttcgctt tacaggaaag tcgataccat cataagaatg
     1321 gttaccgtag ataccaatac ggttaagaag cgttttttca atacatattt cgaagaaaaa
     1381 cccacagaaa ttaaagctcg agaagcagcc gacatggtgt caagaattcg tgaaagccgg
     1441 tttaatttgt tgacaggtta tcaggaaacc agttattcgc ctgaaactat gaagtatatg
     1501 gatgaaaaac ttcaattaat ggctcaggaa tatatttctc ttttcaaggg attgaaaaca
     1561 gagaacctgc acacctatac ctattatatt gtacccgatc agaataaatc gaatcaggtc
     1621 atttgtaaat tttcgaagga aacaggtatt tccgatgcca ccgatagcaa agggaaagaa
     1681 atcgtactta gcataaaaag aagcggaact atgattcaaa tgcctgcctg ggcttctgca
     1741 cccgttgagg taaaatctct tttagttcct tatcgtattc ctgaaatggt gaggttatca
     1801 gtaaattatg ttggaaaggt ttatgatgaa caagtgatag ctctccctca atttggtgta
     1861 gtttcggttg tgccttttgt aaagagcaaa atagaattcg atccccaaac aggatgcatt
     1921 gtaaaaatct actatgaata atttctgttc aaggcttttc tgatttgaac gagctgtttc
     1981 aagagatttt cgagcaggga gagttgcagc atattggctc cgtctgataa agctctggat
     2041 ggttcaggat gggtttcgat gaaaataccg tcagcgcttg cggcaacagc tgcttttcca
     2101 atagtagaaa taaattgagg cgagcctccg cttacgccag ctgaggtgtt cggctgctgc
     2161 aacgaatgcg ttatgtcaac tacaacgggc acatcaaaac tttgcatgat gggaatggag
     2221 cgaaaatcaa ctatcaaatc gttataacca aaggtagttc ctctttcggt aaggattatt
     2281 cgattattac cctggctttt gagcttttcg acgacaaatt gcatggcttc gggcgaaatg
     2341 aactgccctt tctttacgtt gattactttt tgcgtaagtg cggctgcaac gagtaattct
     2401 gtttgccggc aaagaaatgc aggaacctgc aatacatcta cataagcagc agccagttcg
     2461 gcttcctcgg ccgtatgaaa atcggttaga actggaatcc ttaattcatt acgcacgtcg
     2521 gcgatggctt tcaaagccaa aatatctcct attcccgtga aggaatgtat gcttgtgcga
     2581 ttggcctttt tataggaagc tttgaaaatg aaaggtattt cgagacgttc acagatatca
     2641 tagattactg aggcaatctc gaggggcatt tgtggatttt cgacaatgca aggccctgct
     2701 atcaggaaga aattatcgga attcttatgc cggagcaagg gtaaaccatc aggtatcata
     2761 attttaattg ggtttcaata tttatatttg tttttccaga gaattttaag acgattttta
     2821 atttcatttt cacgagggtt attgccaggt tcgtaaaata tatttccttg cagcgaagaa
     2881 gggaggaaat ccatagcaac gaagttatc
//
LOCUS       DAORPD010000114         2885 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_6151_length_2885_cov_2.613074, whole genome shotgun sequence.
ACCESSION   DAORPD010000114 DAORPD010000000
VERSION     DAORPD010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6151_length_2885_cov_2.613074"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1287)
                     /locus_tag="PK976_02020"
     CDS             complement(<1..1287)
                     /locus_tag="PK976_02020"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HRC66696.1"
                     /translation="MKIINRAHKKVSMLRKLMLVTFCLGSIQILEAQFTDDFSDGDFS
                     VNPSWTGTSNSFIVNSAKQLQLNAPVEGIAWLSTASSSVEATEWRFWIKLAFSPSANN
                     FARVYLISDEEDLTGPLNGYYLQFGETGSNDAIELFKQQGTNTTSICRGTNGSIASAF
                     AIRIKVVHKPDGEWLIYSDPAGNENFQLQAIGSDVSITAGNRFGIFSKFTSSNITKMY
                     FDDFYTGPIIIDTIPPVLQLLTITSDSSLLLKFSEPLEPSSAQNTTNYLLSPGDIHPF
                     QANFLPNDASSVQLIFSHIFQSGVNYTLSINNLTDLSGNNTGMIQHHFCLYSVKPYDI
                     VINEIMADPEPSVGMPLWEYIELFNTTSVEISLKDWKLKIGNTLKILPEVSMGPQSYL
                     ILCRPEAQTELSAYGITAPITSFLLNNTGQDLQLLDK"
     gene            1432..2430
                     /locus_tag="PK976_02025"
     CDS             1432..2430
                     /locus_tag="PK976_02025"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HRC66697.1"
                     /translation="MKIAIIGATGLVGRKMVEKLGSSFISRDAEVFLAASEKSYGKEF
                     FLQGKTLKVSSIENVLEQKPHIALFSAGNWVSEKYAREFVRQGAFVIDNSSYWRMHDD
                     VPLVVPEINSENITSKTRLIANPNCSTIQMVLVLSGLHKAWCIRRIVVATYQSVTGTG
                     VRALNQLMNERTGMIVSEPAYPHTIDLNLFPHGGNFLPNGYTTEEMKLVNETRKILHD
                     NQLQVTATVVRVPVKGGHSEAVNVEFVEDYDIDDALEILRNTPGVVVQDSPSEDIYPM
                     PLTAEGKDEVFVGRIRHDESCRKALNLWIVADNLLKGAATNAVQIAEYIVNRKYIQ"
     gene            2434..>2885
                     /locus_tag="PK976_02030"
     CDS             2434..>2885
                     /locus_tag="PK976_02030"
                     /inference="COORDINATES: protein motif:HMM:NF018299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HRC66698.1"
                     /translation="MEIYRYSDHLPAFNKPVVTVGTFDGVHLGHRRIIDRLKEIARQI
                     QGETIVIPFEPHPRLVLAQYGTPVKLINSTYRKYQLLSDLGIDHLIILPFSVEFSMTP
                     WEVFIREYIINQIHTSYLVVGYDHRFGHDRQGDRSKLQAFANELGLNI"
BASE COUNT          841 a          547 c          643 g          854 t
ORIGIN      
        1 tttgtctaaa agctgcaggt cttgccccgt attattgagt aaaaatgaag taatgggtgc
       61 ggtgatacca taggccgata gttcagtttg agcctctggc cggcacaata tcaaataaga
      121 ttgagggccc atactaacct cgggaagtat tttcaaggta ttaccaattt ttaatttcca
      181 atctttaaga gagatttcaa ccgaagtggt attgaataat tcgatgtatt cccacagagg
      241 cattcctacc gaaggttcgg ggtctgccat aatttcgttg atgacgatgt cgtaaggctt
      301 taccgaatac aagcaaaaat ggtgttgaat catgcccgta ttgtttcccg acaaatctgt
      361 taaattattg atagatagag tataattcac gcctgactga aaaatatgtg aaaatataag
      421 ttgaactgaa ctcgcatcgt taggtagaaa atttgcctgg aacggatgaa tatcgccggg
      481 agaaagcaaa taattcgtcg tgttttgagc cgatgatggt tctaagggtt cagaaaactt
      541 gagcagaagg ctgctgtcgg aagtgattgt taacaactgc aaaacaggag gaatggtatc
      601 tatgatgatt ggtccagtat aaaaatcgtc gaaatacatt ttggtgatat tgctcgaggt
      661 aaattttgag aatattccaa acctgtttcc tgcagtgata ctaacatctg agccaatagc
      721 ctgtaactga aagttttcat ttcctgcggg gtcagaatat attaaccatt caccatcggg
      781 cttgtgaact acttttatgc ggatagcaaa ggccgatgct atgctaccat tggttcccct
      841 acaaatggag gtagtatttg tgccttgttg tttgaagagt tcgatagcat cattcgaacc
      901 tgtttctcca aactgcaaat aataaccatt caagggtcca gtaaggtctt cctcatcgga
      961 aatcagataa actctggcga agttatttgc agaaggacta aatgccagtt tgatccaaaa
     1021 tctccattcg gtagcttcta cgctcgaaga agcagtcgag agccaggcaa taccttccac
     1081 aggtgcattc agctgcaact gtttggcgga atttacaata aaagaattag aagttcctgt
     1141 ccatgatgga tttactgaaa agtccccgtc ggagaaatca tcggtaaatt gtgcttccaa
     1201 tatttggatg gatccaagac aaaacgttac cagcatgagt tttcttagca tgcttacttt
     1261 cttatgcgcc ctgtttatta ttttcattgc ttgagaaaag tttgataaat cttgggttct
     1321 ctttacatcg gtaaatatag taattttgcc catttgtatc atttggcttt cacaaaataa
     1381 ataataatct ctcttcggaa ggaatatatt ttcaatagac agaaccaact catgaagata
     1441 gcaattatag gagctaccgg gcttgtaggc cggaaaatgg ttgaaaaact cgggtcttca
     1501 tttatttctc gtgatgctga ggtattttta gccgcaagtg aaaagtcgta cgggaaagaa
     1561 ttttttctgc aggggaaaac tttgaaagtt agcagcatag aaaatgtttt agaacaaaaa
     1621 ccgcatattg ccttgttttc ggctggaaac tgggtatcgg agaagtatgc cagagaattt
     1681 gtcaggcagg gtgcatttgt gattgataac agcagttatt ggcggatgca tgatgacgtt
     1741 ccattggttg tacccgaaat caattccgaa aacattacct ccaaaacccg cttgatagca
     1801 aatcccaatt gctcgaccat acaaatggtt ttggtcttat cagggcttca taaagcgtgg
     1861 tgtattcgca ggattgtggt agctacctat cagtctgtta cgggcactgg tgttagggct
     1921 ttgaaccagt tgatgaatga acgtaccggg atgattgttt ccgaacctgc ttatcctcat
     1981 accatcgatc tcaatctttt tccacacgga ggtaattttc tgcccaatgg atatactacc
     2041 gaggaaatga aactggtgaa cgaaacccga aaaatcttac acgataacca gctacaggtt
     2101 acggctacgg tggtgagggt gcctgtaaag ggggggcaca gcgaagccgt gaatgtcgaa
     2161 tttgttgagg attacgatat tgatgatgct ctcgagattt tgagaaatac gccaggcgtt
     2221 gtggttcaag atagtccttc tgaagacatt tatcccatgc cattgaccgc tgaaggaaaa
     2281 gatgaagttt ttgtagggcg catacgccat gatgaatctt gtcgaaaagc actcaatcta
     2341 tggattgtag ccgataactt attgaaagga gccgccacta atgcagttca gatagccgaa
     2401 tacattgtca atcgaaaata tattcaataa gaattggaaa tatataggta ttctgatcat
     2461 ttacccgctt tcaataaacc ggtagttact gtgggtactt ttgatggagt tcatcttggc
     2521 caccgtcgga tcatcgatcg tttgaaagaa attgcccggc aaatacaggg tgaaactata
     2581 gtaattccct tcgagcctca tccgaggctt gtattggccc aatatggaac cccggtaaaa
     2641 ttgatcaatt ccacttatcg gaaataccag cttttatccg acttgggtat agatcatttg
     2701 attattcttc cctttagcgt ggaattttcg atgactcctt gggaggtttt tatacgcgaa
     2761 tatattataa accagattca cacatcctat ctcgtagttg gttatgatca ccgtttcggg
     2821 catgaccggc aaggcgatcg ttcgaaactt caggcttttg ccaacgaact gggtttgaat
     2881 atcga
//
LOCUS       DAORPD010000115         2869 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_6198_length_2869_cov_2.356787, whole genome shotgun sequence.
ACCESSION   DAORPD010000115 DAORPD010000000
VERSION     DAORPD010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6198_length_2869_cov_2.356787"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            405..1886
                     /locus_tag="PK976_02035"
     CDS             405..1886
                     /locus_tag="PK976_02035"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HRC66699.1"
                     /translation="MNRNIYLLFSILSIFICLLDIIEVNAQETPGLSVGNYTGIHALQ
                     SNPSAAIYSAYWLDFNIIGLEVFACNNGFYIPRSDFNVTDLLRRDYVFPTYGKYNRSF
                     LTKGGTFMRNFFTQNRVLLPSAMVVDITRGLSVGLLLQSREAASGDCIPYDIANFGYY
                     GLEHKAQHNIEYNDYNIYTAGLVWTELGVNIGRTLKRERFDLWAAGITLKYLWGHSGY
                     FARIDNVRYVVLNDSTMDIRNLDAKAGFSLAIDYSNNDFPGPEGYTLGHGFSFDLGIT
                     FIKTNRELRRLFPGRLCEARFEPYVFRLGLSLLDIGSIRFSRNAQKHVFDGVSHFWEH
                     ARDFTYTTVDEAVNDISNRFYGNPTASLNDTSFTVGLPTAFSIQFDYHLNEKYYVHAM
                     AIQAFPILGEEAVKRANVIYVMPRYETPAFEIGIPLSLYRYRIPRLGFWFRIYSFTLG
                     TDNLGMFLRTGDFNNADIYLSLRIPLRKGKCLKSKGKLPCESF"
     gene            complement(2040..>2869)
                     /locus_tag="PK976_02040"
     CDS             complement(2040..>2869)
                     /locus_tag="PK976_02040"
                     /inference="COORDINATES: protein motif:HMM:NF019869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRC66700.1"
                     /translation="NYSAVACRWFENRFNKSLLIIEDHFGKYRISDALMEAYRLIWDD
                     FCSWLLEIVKPPYQHPMDKITYQLVNGFFDNLLRILHPFMPFITEEIWHHLQTRSKND
                     TIMLAPWPDTHEVDETLLNDFDFAREVIVNIRNLRQNQNIPPKKMIELYYIDSSQSNL
                     KPDFKSIITKLSNLSKIAPLSANMENALTFIVKSTEFYVPAPQNFDLGNERKKLMEEL
                     EYTKRFLSSVNSKLNNEGFRKHASQKVIENELKKKADAEERIRLLREKLDQLNRHSK"
BASE COUNT          792 a          516 c          587 g          974 t
ORIGIN      
        1 ggttcggaag gtaaatcacc ccggctggaa acatcagatg ccctgtttcc tcctgatttt
       61 ccgtaaataa acagaataaa gaaaaaggcc aacccgtact taaggttttt catctttcgt
      121 tcgtggtttg tccatttcta attagaacaa tttgcaaaga taagcaaact attatttttc
      181 ttttcattat tgctgcctga ttttttccaa tttttatcca cattttcata ataatggttt
      241 tcttaatgca ttggatatta ttctattact tttattacag aacgtatgtt gtttgcgtaa
      301 aaaacattcc tgatgaaaac acgttataat cttggggaat ccaaacaacc tgagcttttt
      361 ggctttgtct attaaaaaat gaagtaaaag actgcaaaat gcttgtgaat aggaatatat
      421 atctcttatt cagtatatta tcgatattca tttgtttatt ggatatcatc gaagtgaatg
      481 ctcaggaaac gccaggactt tcggttggaa attatacagg aatccatgcc ttgcagtcga
      541 atccttcggc agctatttac tcggcttact ggctcgattt taatattata ggactggagg
      601 tttttgcttg caacaatggc ttttacatac ctcgctccga ttttaacgta accgatctat
      661 tacgcaggga ttatgttttt ccgacgtatg gtaaatacaa ccgttctttt cttacaaagg
      721 gaggaacatt tatgcgcaat ttttttactc agaatcgtgt cttgctcccc tcagcaatgg
      781 ttgtagatat taccagggga ttgagtgtag gtttacttct ccaatcacgc gaagctgctt
      841 cgggcgactg cattccgtac gacatcgcta attttggata ttatggactt gaacataagg
      901 ctcaacataa tattgaatac aacgattaca atatttatac cgcggggctt gtgtggacag
      961 aacttggggt gaatattggc cgcaccttaa aaagggaaag gtttgattta tgggcggccg
     1021 gcatcacatt gaaatatcta tggggtcatt caggttattt tgcaagaatt gacaatgtcc
     1081 gctatgtggt attgaacgat tctaccatgg atattcgcaa ccttgacgcc aaagctggtt
     1141 tttcactggc tattgactat agcaataatg atttccctgg tccggaaggt tatactcttg
     1201 gtcatggatt cagcttcgac cttggaataa cttttatcaa aacaaatagg gaattgagga
     1261 gacttttccc gggcaggctt tgtgaggcac gtttcgagcc ttatgttttc agattgggct
     1321 tgtcattgct tgatattggg agtattcgct tttcacgaaa tgcccagaaa catgtattcg
     1381 atggagtaag ccacttttgg gaacatgccc gggacttcac ttatactacg gttgatgaag
     1441 cggtaaatga cataagcaac cgcttctatg gaaatccaac tgcttcgttg aacgatactt
     1501 catttactgt gggcttgcca acagccttta gtattcagtt cgattatcat ttgaatgaga
     1561 aatattatgt tcatgccatg gcgattcaag ctttccctat attgggtgaa gaggccgtta
     1621 aacgtgcaaa tgtcatttat gttatgccaa gatatgaaac tccggcattt gaaattggaa
     1681 ttcctctgag tctctatcgt tatcgaattc cacggttggg cttttggttc cggatatatt
     1741 cctttaccct tggtacagat aatctgggta tgtttctgcg aacgggcgac ttcaacaatg
     1801 ccgatattta tctatccttg agaattccct tgagaaaggg aaaatgtctg aaatcgaaag
     1861 gaaaattgcc ttgcgaaagt ttttgattgc tgtaaaccga gaatgcgtaa ggtttcgaat
     1921 catgcccctt ctgaaataaa cgattctatt tttctaaaaa aatgtttctt cttacaatcc
     1981 atttttttaa cggcttttgt atagcgtaag attcttccaa gatattcatt gtattttctt
     2041 tatttggaat gtctgttcaa ctgatcgagt ttttctctca atagtcgaat tcgttcttcc
     2101 gcatcagcct tcttttttag ctcgttttcg atcacttttt gtgaagcgtg tttcctgaaa
     2161 ccttcattat ttaattttga attcactgat gaaagaaatc ttttggtata ctcgagttct
     2221 tccattaatt tttttctttc gtttcccaga tcgaaatttt gaggagcagg aacataaaat
     2281 tctgttgatt ttacaataaa tgtgagcgca ttctccatat tagccgaaag aggtgcaatt
     2341 ttcgatagat tggaaagttt ggtaatgatg gatttaaagt cgggtttcag attagattgg
     2401 cttgaatcga tgtaatacag ttcgatcatc ttttttggag gaatattctg attttgacgc
     2461 agattacgga tattgacgat aacttcacga gcaaaatcga aatcattcag caaagtttca
     2521 tctacttcat gagtatctgg ccatggagca agcataatag tatcgttttt agaacgggtt
     2581 tggagatggt gccatatttc ttcggtaatg aagggcataa atggatgtag aatccgtagt
     2641 aagttgtcga aaaagccatt cacaagctga taagtaattt tatccattgg atgttgatag
     2701 ggaggtttga ctatttcgag caaccatgaa cagaaatcgt cccatatcag gcgataagct
     2761 tccatgagag cgtctgaaat gcggtatttt ccgaagtggt cttcgataat gagtaatgat
     2821 ttgttgaacc tattctcgaa ccatcggcac gcaacggccg aatagttgg
//
LOCUS       DAORPD010000116         2862 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_6221_length_2862_cov_2.349840, whole genome shotgun sequence.
ACCESSION   DAORPD010000116 DAORPD010000000
VERSION     DAORPD010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6221_length_2862_cov_2.349840"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..886
                     /locus_tag="PK976_02045"
     CDS             152..886
                     /locus_tag="PK976_02045"
                     /inference="COORDINATES: protein motif:HMM:NF040157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5714 domain-containing protein"
                     /protein_id="HRC66701.1"
                     /translation="MSDNKNGCLICGEDLVYNDILEKTMCFYCKKIFETNVICKNQHY
                     VCDGCHSLPAYLAIETFCLMNMLKDPLEIAILLMQHPSIKMHGPEHHFLVAASLLTAY
                     YNTINEPSKKSVILTEAQKRAKNILGGFCGYYGNCGAAVGTGIFLSLITQATPLSKKE
                     WELCNLMTSNSLFSIAHAGGPRCCKRNTYLAVIEAARFVNENFQISLKTGKSLQCSFS
                     SFNRECKKEDCKFYKGSRINVLIEKN"
     gene            complement(1103..>2862)
                     /locus_tag="PK976_02050"
     CDS             complement(1103..>2862)
                     /locus_tag="PK976_02050"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRC66702.1"
                     /translation="PSWTSNVIWYQIFVERFYNGNPNNDPTPEDISVSSSTFPLPDDW
                     TITPWTMDWWKREPWAEKTGKSHRETVQHRRYGGDLQGVIDKLPYLHELGITATYFNP
                     LNDAPSLHKYDARNYHHIDVNFGPDPKGDKKIIASEIPSDPSTWKWTAADRQFLQLIE
                     QCHQMGIRVIMDYSWNHTGVDFWAWKDVLQNLQKSPYRDWYEIISFDNPLTPENEFQY
                     RGWANVASLPEWKKVNLPGPRKSNGPYEGNLNPKVKEHIFAVTQRWLAPNGDKTKGID
                     GFRLDVAEQIPLGFWRDYRKWVKSIKPEAYLVGEIWWEKWPDKLMDPAPYVNDEIFDA
                     VMFYKIYAPARSFFVHGKEALTSSQLADSLQKEWTNLPKNFRYTQMNVNATHDSPRLL
                     TCFANKGKYKYHVNPYENPRCITGKPDKDTYNRVRLYLMHQFTIPGSPSIWNGDEMGM
                     WGSDDPDNRKPLWWPDTKFEPETRTNIQPGFKLYDEVKFDADHYHFYKQIISLRKNNP
                     VLATGEIEFISSEDRVLAYRRWNSSDEIFVCLNTDTTSHRIKFASKGKFVDLLSGRII
                     KGKSIPILPLEGLILRRVE"
BASE COUNT          858 a          576 c          552 g          876 t
ORIGIN      
        1 atgacaaaaa ttttaaaatt agtaaattcc tgtccagttt ttttagacca tctcaggtag
       61 aagaaaaaaa agatagtcaa gcttaaagtc aatattgaat aaataaattt attaatttcg
      121 ctcaataata aattgcatct ctcgagatat catgtcggat aataaaaatg gctgtttgat
      181 ttgtggagaa gacttagttt acaatgatat actcgaaaaa accatgtgct tctattgcaa
      241 gaaaattttc gaaaccaacg taatttgcaa aaaccagcat tatgtttgtg acggatgtca
      301 tagtttgcca gcctatttag cgatcgaaac attttgcttg atgaatatgc tgaaagatcc
      361 actcgagata gctattttat tgatgcagca tccatctatt aaaatgcatg gaccagaaca
      421 tcactttctt gtggcagcat ctttgcttac agcttactat aataccatca atgaaccgtc
      481 aaaaaagtcg gttatactca ccgaagccca aaaacgtgct aaaaatatcc ttggaggttt
      541 ttgtggatat tatggtaatt gtggtgctgc cgtgggtacc ggcatttttt taagcttgat
      601 tacccaggca actcctttat ccaaaaaaga atgggaactt tgcaacctga tgacatccaa
      661 cagtcttttt tcgattgctc atgcgggagg tcctcgctgc tgcaaacgca acacctatct
      721 ggctgttatc gaggctgctc gttttgtaaa tgagaatttc caaatttcac tcaaaacagg
      781 aaaatctctt cagtgttcat tctcttcgtt caatcgagaa tgcaaaaaag aagactgtaa
      841 gttctataag ggttcccgca tcaatgttct aattgagaaa aattaacctt tttcatcttt
      901 ttattaaaat gtttcttaac cgaaacaaaa cgagtgaaaa tcaacaataa atggcatacc
      961 accaaaatta caaagaaaac tactgacttt ttgaaaaatc agtactatct attacgtaaa
     1021 ttttgcgtct cttcgtctcc tcatggtgtc gcctcaagaa tttaaaggtc tcctcgaacc
     1081 tggggacttg catcatatta acttactcca ccctccgaag aatcaatcct tcaagaggca
     1141 aaataggaat tgatttgcct ttaatgattc ttccagaaag taaatccaca aatttccctt
     1201 ttgatgcaaa cttgatgcga tgagaagtgg tatcggtatt caaacaaaca aatatttcat
     1261 ctgaagaatt ccagcgtcta tatgccagta ctcgatcttc tgaagaaata aattcaatct
     1321 cgcccgtagc cagaacaggg ttatttttcc gtaaactgat gatttgctta taaaagtgat
     1381 aatgatcggc atcgaatttc acttcatcgt ataatttaaa gccaggctga atattggttc
     1441 gagtttcagg ttcgaatttc gtgtcgggcc accataaagg tttacgatta tcgggatcgt
     1501 cgctgcccca cattcccatt tcatcaccat tccaaataga aggagaaccg ggaatagtaa
     1561 attgatgcat gagatacaat ctaactctat tgtaggtatc tttgtcgggt ttacctgtta
     1621 tgcatcgagg attttcgtaa ggattgacat gatatttgta ttttcctttg ttggcaaaac
     1681 aagtcagcag acgtggacta tcgtgtgtag catttacatt catttgagtg taacggaaat
     1741 ttttcggcag atttgtccat tctttttgta aactatcggc caattgcgaa gaagttaagg
     1801 cttcttttcc gtgaacgaag aagctacgag ccggtgcata aatcttatag aacattactg
     1861 cgtcgaaaat ctcatcattt acataaggtg caggatccat aagcttatcg ggccattttt
     1921 cccaccagat ttctcccaca aggtaagcct cgggtttaat agattttacc catttacggt
     1981 aatctctcca aaaacccaga ggaatttgtt cggccacgtc gaggcgaaaa ccatctattc
     2041 ccttggtttt atctccatta ggagcaagcc atcgctgagt aacggcaaaa atatgttcct
     2101 ttaccttagg attcaaattt ccttcgtagg gaccattgga ttttcttggc ccaggtaagt
     2161 taactttttt ccactccggg aggctggcca cattagccca accacgatat tggaactcat
     2221 tctcaggagt aagggggtta tcgaaagaaa tgatctcata ccaatcacgg taaggtgatt
     2281 tctgtaaatt ttgcagaaca tctttccatg cccagaaatc cacacctgta tgattccacg
     2341 aataatccat aatgactctt atgcccattt ggtggcattg ttcgattaat tggaggaatt
     2401 gcctatcggc tgccgtccat ttccaagtag aaggatcaga tgggatctcg gaagcaataa
     2461 tttttttgtc acccttagga tcgggaccaa aattcacatc tatatggtgg taattgcgcg
     2521 catcatattt atgcaaagaa ggtgcatcat ttagtggatt gaaataagta gcggtaattc
     2581 ccaattcatg cagataggga agtttatcga taacgccttg cagatcgcct ccataacggc
     2641 gatgctgaac ggtttcccga tgggattttc ccgttttttc tgcccaaggt tctcgcttcc
     2701 accagtccat cgtccatggc gtaattgtcc agtcatcggg gaggggaaag gttgacgagc
     2761 tcaccgagat gtcttcggga gtaggatcat tattagggtt tccattgtaa aagcgttcta
     2821 cgaaaatttg ataccaaatc acattggaag tccatgaagg ag
//
LOCUS       DAORPD010000117         2861 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_6222_length_2861_cov_2.426230, whole genome shotgun sequence.
ACCESSION   DAORPD010000117 DAORPD010000000
VERSION     DAORPD010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6222_length_2861_cov_2.426230"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..102)
                     /locus_tag="PK976_02055"
     CDS             complement(<1..102)
                     /locus_tag="PK976_02055"
                     /inference="COORDINATES: protein motif:HMM:NF013545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HRC66703.1"
                     /translation="MSGSLRENVGKKDAKKLRKNGLVPANIYGGANQF"
     gene            complement(206..1150)
                     /locus_tag="PK976_02060"
     CDS             complement(206..1150)
                     /locus_tag="PK976_02060"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HRC66704.1"
                     /translation="MARNKVKIFSGRSSKYLAEEIAKVYGDELGKVNLTTFSDGEFQV
                     SFEQTVRGNDVYIVQSTFSPVDNLFELLMMIDAAKRASAERIVAVVPYYGFARQDRKD
                     KPRVAIAAKLVADLLSTAGVGRIITMDLHSDQIQGFFNVPVDHLYASTIFVPFIKKLK
                     LPNSVMASPDTGGAKRAAAYAKFLNTELVICFKQRIKPNSIGKMTIIGEVEGKDVILV
                     DDIIDTAGTVCKAADLMMEHGANSVRVFATHAVLSGNALENIHKSSLIEVYVTDTLPV
                     KETEKIKVLSIAPLLADVIDRVHRNKPISSHFDFTTIL"
     gene            1407..1489
                     /locus_tag="PK976_02065"
     tRNA            1407..1489
                     /locus_tag="PK976_02065"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1441..1443,aa:Leu,seq:caa)
     gene            complement(1678..>2861)
                     /gene="tyrS"
                     /locus_tag="PK976_02070"
     CDS             complement(1678..>2861)
                     /gene="tyrS"
                     /locus_tag="PK976_02070"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005843969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HRC66705.1"
                     /translation="YIGFDPTADSLHIGHLVQIMTLVHFQQCGHKPIALVGGATGMVG
                     DPSGRSEERNLLSREQLEYNLVGIKKQLARFIDFETKVNPATMVNNYDWFKGYEFLDF
                     IRDVGKHITVNYMLAKDSVQKRLDAGMSFTEFSYQLVQAYDFYWLFNNMDCRLQMGGS
                     DQWGNIVTGTELIRRKDGGTAYALTTPLLKKSDGSKFGKTAAGALWLDAEKTSPYAFY
                     QYWINTSDEDAIDYIKIFTTLSKEEIDALSEEHWKTPHLRILQKALAREITIRVHSEN
                     DYQMAVEASEILFGKGTVESLMQLDEKTLLNCFDGVPQFHVSKTELETGIPAIDVLAV
                     KTQIFPSKAEARRMMKDGAVLLNKQKLSEDTFIDNSMLLQNKYLLIQKGKKNYFLVIA
                     E"
BASE COUNT          875 a          579 c          520 g          887 t
ORIGIN      
        1 gaattgattt gcacctccat aaatattagc cggcacaagt ccatttttac gtaatttttt
       61 agcatctttc ttccctacgt tctctctgag agaaccgctc atagaaattg ttttcataat
      121 caaaaactta aatgataaaa taaggtttga aaaagtttgc aaaattagct attaaaatat
      181 gattctcaaa caatacagaa atcaatcaaa gtatggtagt gaaatcgaaa tgtgaagaaa
      241 tgggtttatt acgatgaacc cggtcaatta cgtcggcaag caagggagcg atggaaagca
      301 ctttaatctt ttcggtttcc ttgacgggta aagtatctgt aacatagact tcaataaggg
      361 aggatttatg aatattttcg agtgcatttc ccgaaagaac agcatgagta gcaaatactc
      421 tgacactatt ggctccatgt tccatcatca gatcagcagc tttacagacg gttcctgccg
      481 tatcgatgat atcatccacc aaaatcacat cttttccctc tacctcgcct atgatagtca
      541 tttttccaat agaattaggt tttattcttt gcttgaaaca aatgaccagc tcggtattca
      601 ggaatttggc ataagctgct gcgcgtttcg ctcctcctgt atctggagaa gccatgactg
      661 aattgggaag tttcaatttt tttatgaaag gcacgaaaat agtggaagca tacaaatgat
      721 cgacgggtac attgaaaaat ccctgaatct gatcggaatg cagatccatt gtaattattc
      781 ttccaacacc agctgtggaa agcagatcgg caaccaattt ggccgcaata gctacacgag
      841 gcttgtcttt gcgatcttgt cgggcaaatc cataataggg cacgacggca acaattcttt
      901 cggccgaagc tcttttggct gcatcaatca tcatgagaag ctcgaataaa ttatctacgg
      961 gagaaaatgt cgattgaaca atataaacat cattccctct cactgtttgc tcaaacgaaa
     1021 cttgaaattc cccatcgctg aatgtcgtca aatttacttt ccccaattca tccccataaa
     1081 cctttgcaat ttcttcagca agatacttac tcgatcgccc tgaaaaaatc ttaactttgt
     1141 ttctggccat gtttttcaga tagttatgtt ttttgaaggt gttctctgca aaaatactat
     1201 aattcaaaaa gattcaaatc gtttaagaac tcgtatcatt gcgagtgaat cttatctttt
     1261 aaaggtatcg aacttaggga aaagaaactg aatctttttg caggaacatc ctgaagtcga
     1321 aaatatcgat gatgatttta aactgaaaat atcttcacgg gttgaaaatt atggtaagat
     1381 gtttaatttt gcaagttctt tgacatgccc ggatggcgga acaggtagac gcgttggtct
     1441 caaaaaccaa tgggattttc ccgtgccggt tcgattccgg ctccgggtac attatagaag
     1501 atttattata attttctcgt tggctaactt ttatggctaa tgtttagaaa acgtggtaag
     1561 atacaagatt cacccctctt tattgattta tcttctttat ccctatcctt gagctcaatc
     1621 atgaaaaacc tataattata ggataggaag gccaaattaa actcagaaaa aatggtatta
     1681 ttcggctatc acgagaaaat aatttttttt ccctttttga ataagtaaat atttattttg
     1741 aagaagcatt gagttatcaa tgaaagtatc ctcagaaagt ttttgtttgt tgagcaatac
     1801 agcaccatct ttcatcattc ttcgagcttc ggccttactt gggaaaatct gagttttcac
     1861 agcaagaaca tcgattgcag gaatgcctgt ttcgagttct gttttagaaa cgtgaaattg
     1921 tggcacccca tcgaaacaat tcaacaaggt cttttcatcg agttgcatca atgattctac
     1981 agtgccttta ccaaaaagaa tctccgatgc ttcgactgcc atctgatagt cattttctga
     2041 atgcacccga atggtaattt ccctggcgag agccttctgt aaaatacgta gatgaggagt
     2101 tttccaatgt tcttctgaaa gggcatctat ttcttcttta cttaaagttg taaaaatttt
     2161 tatataatct atggcatctt catctgaagt atttatccaa tattgataaa aagcataagg
     2221 agaagttttt tcggcgtcga gccaaagcgc tcccgcagcc gttttgccga atttgctccc
     2281 atccgatttt ttcaataatg gggttgtaag agcataagcc gtcccaccat ccttacgccg
     2341 gattaattca gtcccagtaa caatatttcc ccattggtca gaaccgccca tctgaagacg
     2401 acaatccata ttattgaata accagtaaaa atcataagcc tgcaccaatt gataagaaaa
     2461 ctcagtaaac gacattcccg catcgaggcg tttctggaca gagtcttttg ccagcatata
     2521 gtttactgtg atatgtttgc ctacatcacg tataaaatcc aggaattcgt atcctttaaa
     2581 ccaatcataa ttattgacca tagttgcagg attcactttg gtttcgaaat caataaagcg
     2641 ggcaagttgt ttttttatac caacaagatt atattctaat tgttccctgc ttaaaagatt
     2701 acgttcttca cttctgccag aaggatcacc caccatacct gtcgcaccac ccaccaaagc
     2761 aatgggttta tggccgcatt gctgaaaatg aaccaaggtc attatctgca ccagatggcc
     2821 aatatgaagg gaatcggctg tggggtcaaa tccaatatag c
//
LOCUS       DAORPD010000118         2854 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_6239_length_2854_cov_2.501965, whole genome shotgun sequence.
ACCESSION   DAORPD010000118 DAORPD010000000
VERSION     DAORPD010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6239_length_2854_cov_2.501965"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2140
                     /locus_tag="PK976_02075"
     CDS             <1..2140
                     /locus_tag="PK976_02075"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HRC66706.1"
                     /translation="VDAFNIDLKGFTDKWYRDVTKSNGGWSLEQIDPLSPCLGSKNWI
                     ASINISGGTPGGINSVYSTNQLFPLPEMVIPIPPHQLNVVFNQSMDSLSLTNLANYSL
                     LPKFGNPSSIELVDSDFTTLILYYSESFDLSGSYRLVFSTQLFNCIGQAIPENIYWDF
                     NIPSPSKIFDVLITEVMADPDPPIHLPNAEYVEIYNCCDYTVDLSGWSFSIENTDHVF
                     DRYYLKANEYLILAHEKYASDFATYGNFYGFSSFSLPNTGQSLVLKNGKGEAIHWVDY
                     DQNMYGKSPKKDGGWSLEMIDTENPCTIESNWIPSIDPHGGTPGRKNSVSASLPDTIS
                     PRFSRIYPLSPKKIWLYFSEPVKQFEATNPENYVLSDGLTFLKIQAIDHRNKVFEAEL
                     SSELKPAIIYTVSAKNGITDCAGNCLSNNKEIIFGLPLRPARGKLVINEILTNPLEGG
                     SDYLEVYNSSDTLSDLGELALSYISSSGSSTTIFLPPFLLLPSEYYCLTRNPLSVINQ
                     YPLHQKDNFVQVKNFQDLSSSGGTLILYLKEDSTKVIDRFSYSSSMHLPILKNFDGVS
                     LERINPLRPTDDPTNWTSASEASGFGTPAHENSQYSSLPVAKGSLTIEPEIFSPDGDG
                     IDDIVNITIQPPAEGYITNVTLFDSRGRLVKHLVKNLIVGNKCTISWNGMRYDNSRAP
                     VGFYIIYCELFDTNGSTEKHKATVVVGAKL"
BASE COUNT          881 a          600 c          533 g          840 t
ORIGIN      
        1 tgtcgacgcg ttcaacatcg acctcaaggg gtttaccgac aaatggtatc gcgatgtcac
       61 caaaagcaat ggcggatggt cgctcgaaca gatcgatcct ttatccccgt gtctgggaag
      121 taaaaactgg atagcatcaa taaatatttc aggaggaaca ccaggcggga tcaattcggt
      181 ttattccacc aaccagttat tccctttgcc cgaaatggtt atacctatcc ctccccatca
      241 gcttaatgtg gttttcaacc agagcatgga cagtctttct ttgacgaatc ttgcaaacta
      301 ttcccttttg cccaaattcg gaaatccttc cagtatagaa ttggtagact ccgactttac
      361 cacgttaatc ctctactaca gtgaaagttt cgatttatca ggctcttacc gtctcgtttt
      421 ttccacccaa ttgttcaatt gcataggcca ggccataccg gaaaatatct attgggattt
      481 taatatccct tctccaagta agattttcga tgtattgatt accgaagtta tggctgaccc
      541 cgatccaccc attcatctgc ccaatgcaga atatgtcgaa atctacaatt gctgtgatta
      601 tacggtcgac ctttcgggtt ggagctttag tatagagaat accgatcatg ttttcgatcg
      661 atattattta aaagccaacg aatatctcat actcgcccac gaaaaatatg cttctgattt
      721 cgcaacttat ggaaatttct atgggtttag tagcttttcg ctacctaaca ccgggcaatc
      781 gttggttttg aagaatggaa agggagaagc cattcactgg gtcgactatg accagaatat
      841 gtatggtaaa tcacctaaaa aagatggagg gtggtcgctc gaaatgatag ataccgaaaa
      901 cccctgtact attgagagca attggattcc ttccattgac ccacatggag gaactcccgg
      961 acgaaaaaat tcggtttcgg cttcattgcc cgatacgatt tctcctcgtt tttcacgaat
     1021 ctatcctctc agtccgaaaa aaatatggct ctatttttca gaaccggtaa aacaattcga
     1081 agccactaat ccagagaatt atgtcttgag tgatggactt actttcttga aaattcaagc
     1141 catagatcac aggaataagg ttttcgaagc cgaactttcg agcgagctta aacccgcaat
     1201 tatttatacg gtttctgcta agaatggcat taccgattgt gctggcaatt gcctttcaaa
     1261 taataaggaa ataatatttg gtctacccct gcgaccagcc agagggaagc ttgtcatcaa
     1321 cgaaattttg acaaacccat tagagggtgg aagcgattat ctcgaagtat ataattcctc
     1381 cgatactttg agtgatcttg gagaactagc cttaagttat atttcttcat caggaagctc
     1441 aaccaccata tttcttccac cttttctttt gttgccttcc gaatattact gtcttacacg
     1501 caatcctcta tcagtaatca atcaatatcc tctccatcaa aaagataact tcgtacaggt
     1561 gaaaaatttt caagatctgt caagctcggg tggtacgctg attttatatt taaaggaaga
     1621 tagtacaaag gtgatcgatc gattttcgta ttcatcgagc atgcatctcc caattcttaa
     1681 aaacttcgat ggagtatcct tggaacgcat caatccctta cggccgactg acgacccaac
     1741 caactggact tcagcttcag aggcctcggg atttggaaca cctgctcatg aaaactcgca
     1801 atattcatct ttacctgttg ctaaaggcag cttgaccata gaacccgaaa tattctcacc
     1861 agacggcgat ggaattgacg atatagtgaa tattaccatt caacctcctg ctgaaggtta
     1921 tattacgaat gtgacacttt tcgattccag aggcagatta gtaaaacatc tagtaaaaaa
     1981 cctgattgta ggcaacaaat gtacaatttc atggaacggc atgcgctacg ataattcgag
     2041 agcacctgtg ggattttata tcatttattg cgagttgttc gatactaatg gaagtaccga
     2101 aaaacacaag gcaactgtgg tggtaggagc taaactctga gaagctttat ccaaatctta
     2161 cttcttacat aaaaatgaaa tttatacatt cgaagaccaa ggttaatttt tatcagaaat
     2221 tgtctgatat ttataataaa atgtcctgcc atgataagaa tattttttga aaaacatact
     2281 catgcttaaa ttcagaactc ttgatcccgt aattgcctat cgatatttta caaaagtatt
     2341 atcttagaaa cgctaacaaa aattatttca aatcgttagt tcactttgcg cttatgcata
     2401 caattctttt cccgctaaca ggctaagaac cttttagatt ttaacgtcgg gaaaagaaat
     2461 tcaaaaaaga attagcgctt gcagatgatg ggagtcaacc tcaagagggt gaaaagaggg
     2521 attatttaga gcaggtaatc ctttaacgcc atcttcaaaa ccacgacaac ctgaataaaa
     2581 ctgaatcagg atttttgaaa ctgacttggt ggtttactgt ggtttgtcca acgaaccatt
     2641 tggtgtcggg caagtcgaca tttaaaaaaa tatatttaga tgcgcttgtt cgaagagagg
     2701 cagagtaata gaaaaacata agaattatcg aaaattccga cttcatcttc ttttcggtac
     2761 agccaaaatc aaagcctggc gcagttcggg ttcctttctc aaaacaataa tgaaaaacaa
     2821 tacgaatagt atccctttta ttaccagatc gagt
//
LOCUS       DAORPD010000119         2844 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_6267_length_2844_cov_2.681965, whole genome shotgun sequence.
ACCESSION   DAORPD010000119 DAORPD010000000
VERSION     DAORPD010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6267_length_2844_cov_2.681965"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="PK976_02080"
     CDS             <1..793
                     /locus_tag="PK976_02080"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HRC66707.1"
                     /translation="VAVKGGEPEVMFDKAIAAMGGMTQFVKAGQKVVVKPNIGWDVAP
                     ERAGNTNPQLVSHIIKRCLEAGAAEVCIFDNPCDNWVKTYKTSGIEYAVKLAGGKMFP
                     ANEERFFKNVNIQGKVLKTAAVHELILNSDVFINVPILKNHGSTLFTGSMKNLMGVVW
                     DRRFWHRNNLHQCIADFPKFKKPDLNVIDAYRVMFRNGPKGLSQADVKLMKSMIISRD
                     IVAADSAASRLIGLDPSEITHIVMANNSKIGTMDLSKLSIQRINI"
     gene            811..2358
                     /locus_tag="PK976_02085"
     CDS             811..2358
                     /locus_tag="PK976_02085"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRC66708.1"
                     /translation="MSIFRIVVAILSFTLITLAFFDYVSKLSFHHFRTILFLQFVPSL
                     LQFIQSPSIITAGFILVILLTLLFGRVYCSWICPLGILQDGLCKLKPHKRKRRNRYQY
                     YQTNKWLKYSILILTFVSILLGSTILLRFIDPYSLYGKMTYQLGSPVVVGVNNLAFDI
                     LQQMGNYSLKPLPYPSFNLSAFVFSIGFLGILIFLVFWRGRVYCTEWCPVGTLLGLVS
                     KFSIFKLRVSESDCTACGLCARNCKAGCIDTKLKTIDFERCVACFNCLRTCSAHAIIF
                     THHQSKIGESNLYHPDIQRRRILGMMITATAGLGLKSAMAQANIGTKAMLPNPKPYPV
                     TPPGSLSLEHFNKTCTACHLCINACPTQVLQPAMLQFGLIGLFQPYLDFHKNFCNYDC
                     VRCGEVCPTGAIKRLNSDDKKLVQIGQAHFFRENCVVYTEETACGACSEHCPTKAVHM
                     VSYKAGLTIPEVNQNICVGCGACEYACPALPNKAIYVAANPIHFQALAPEKSTKIQEE
                     IPEEFPF"
     gene            complement(2521..>2844)
                     /locus_tag="PK976_02090"
     CDS             complement(2521..>2844)
                     /locus_tag="PK976_02090"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HRC66709.1"
                     /translation="EIIPENEIIDFIKVDVEGAEFCVLKGGKNLLKKNKPIILFEFGK
                     GASDYYGTKPSDLFSYLTEQIGLKIFTLDAYLKSKRNISQSEFEHLYISGKEYYFIAA
                     SDIKE"
BASE COUNT          815 a          534 c          549 g          946 t
ORIGIN      
        1 ggttgccgtt aaaggaggcg agccagaagt gatgttcgat aaagcaatag ctgccatggg
       61 gggaatgacc caatttgtca aagctggaca gaaagttgtc gtgaagccaa atataggttg
      121 ggatgttgct cctgaaaggg caggtaatac caatccacaa ctggtaagcc atatcataaa
      181 gcgttgtctg gaagctggtg ctgctgaggt ttgcattttc gacaatccct gtgataattg
      241 ggtaaaaact tataaaacca gtggaattga atacgcagta aaattagctg gaggtaaaat
      301 gtttccagcc aatgaagaaa gattttttaa aaatgtcaat atccagggga aagtattaaa
      361 aacggctgcg gttcatgagc tcatccttaa cagcgatgtg ttcatcaatg ttcctatcct
      421 taaaaaccat ggctctacct tgtttactgg ttctatgaaa aacttaatgg gagttgtttg
      481 ggacaggcgt ttctggcatc gtaacaatct tcatcagtgt atagcagatt ttccaaaatt
      541 taaaaaacct gatctgaacg taatagatgc ttaccgagtt atgtttcgta atggtccaaa
      601 aggtttatct caagccgatg taaaactaat gaaatcgatg atcatttctc gagatattgt
      661 ggctgccgac tcagctgctt cccgcttgat tggccttgac ccaagcgaaa ttacccatat
      721 cgttatggcc aataacagca aaataggaac gatggatttg tcgaaactca gtatccaacg
      781 tatcaatatc tgatgaaagt atcgcataga ttgagtatat ttcgcattgt ggtagccata
      841 ttgtcgttta cactgattac tttggccttt ttcgactatg taagcaaatt gtcgtttcac
      901 cattttcgta cgattctttt tctgcaattc gttccatcac ttttacaatt tatccaatca
      961 ccttcgatta ttaccgcggg ttttattctg gtcattcttc ttactttact gttcgggaga
     1021 gtgtattgtt catggatttg tccattggga atacttcagg atggattatg caagctaaag
     1081 ccacacaaaa ggaaaagaag gaacaggtat cagtattatc aaacgaataa atggctaaaa
     1141 tacagcatac ttattttgac tttcgtgtct attctgttag gttctacaat tttattgcga
     1201 tttatcgatc cttattctct ttatgggaag atgacctatc aattggggag tcctgtggtg
     1261 gtgggcgtca acaatttggc tttcgatatc cttcaacaaa tgggtaatta ttcgcttaaa
     1321 ccattgcctt atccttcttt caatctatcg gcctttgttt tttcaatagg atttttagga
     1381 atattaattt ttttggtttt ttggaggggc agagtgtatt gtacggaatg gtgtcctgtt
     1441 ggaactttgt tgggtttggt ttcgaaattt tcgattttca aactccgcgt ttctgaatcc
     1501 gactgcaccg catgtggact ttgtgcccga aattgcaaag ccggatgtat tgatacaaaa
     1561 ttaaaaacga tcgatttcga aagatgcgtg gcatgcttca actgtttacg cacttgttct
     1621 gctcatgcca ttatttttac gcatcaccaa tcaaaaatcg gtgaatctaa tttatatcat
     1681 cctgatatac agcgtagaag gattttgggt atgatgatta ccgccactgc aggtttagga
     1741 ttgaaatctg ccatggctca ggcaaatatc ggtacaaagg caatgctgcc aaatcctaaa
     1801 ccctatcctg ttactccacc gggttctctc tctctcgaac atttcaacaa aacctgtacg
     1861 gcctgtcatt tatgtataaa tgcttgtcca acacaagttt tacagcctgc tatgctgcaa
     1921 tttggattaa tcgggctttt ccagccctat ctcgattttc ataaaaattt ttgtaattac
     1981 gattgtgttc gttgtggaga ggtttgccct actggagcta tcaaaagatt aaattcagat
     2041 gacaaaaaac tggttcagat agggcaagct cattttttca gagagaactg tgtggtttat
     2101 accgaggaaa ctgcctgtgg agcttgttca gaacattgtc ccacaaaggc tgttcacatg
     2161 gtgtcttata aggcaggcct tacgattccc gaagtaaatc agaatatctg cgttggttgt
     2221 ggtgcatgcg aatatgcctg tcccgctttg cccaataaag ccatatatgt ggctgccaac
     2281 cccatacatt ttcaagcttt agctccagaa aaaagcacta aaattcaaga agaaataccc
     2341 gaagaatttc cattttaata cttcctcgct aagacagtat taataaaatc ccatgattat
     2401 taagagattc tatattgttt gtttatagtt gtttatatat taatttttta aaaaattctg
     2461 tgacttttta ttcagtgaat aatattttat tgctattagc attttgcttc tcacgaaggt
     2521 ttattcttta atatcagatg ccgctataaa ataatattcc tttccagaaa tgtataaatg
     2581 ttcgaattcc gactgactta tatttcgttt gcttttaagg tatgcatcga gagtaaaaat
     2641 tttcaaaccg atttgctcgg tcaagtaact aaataaatcg gaaggttttg tgccataata
     2701 atcgctggcg ccttttccaa attcaaataa tataataggc ttattttttt tcagtaaatt
     2761 tttccctccc ttcaatacgc aaaattccgc tccttccaca tctaccttaa taaaatcaat
     2821 gatttcgttc tcgggaataa tttc
//
LOCUS       DAORPD010000120         2839 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_6283_length_2839_cov_2.225575, whole genome shotgun sequence.
ACCESSION   DAORPD010000120 DAORPD010000000
VERSION     DAORPD010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6283_length_2839_cov_2.225575"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="PK976_02095"
     CDS             <1..744
                     /locus_tag="PK976_02095"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRC66710.1"
                     /translation="NDLNITNEKLAHAKAALKQSEKLAHMGQLSAGIAHELNNPLGVI
                     TMYANIMMEERPENDPLRKDLELIVEQANRCKKIVSGLLNFARKNQVNLTETHLEQLV
                     QRSLDSVVKPDNVKTRMISEMKDPICFLDQEQMMQVLTNLEKNAIEAMPPEGGEITVT
                     LKDNLHEVIIKVADTGIGIEKENMDKLFTPFYTTKELGKGTGLGLPLIYGIVKMHKGQ
                     ISVMSNNNKEEGPTGTIFTITLPRNPNSV"
     gene            772..1164
                     /locus_tag="PK976_02100"
     CDS             772..1164
                     /locus_tag="PK976_02100"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRC66711.1"
                     /translation="MEAHKKTIFIVDDDPDYLFQLQVLLEKNGFRVITAESQREAQKI
                     IERTKPDLAIVDLMMENEDSGFILSYQIKRLYPDVPVIIFTAVTAETGISFETELGMN
                     SRWIKADLFLPKGLRPDQLIREINKLLK"
     gene            1179..2321
                     /locus_tag="PK976_02105"
     CDS             1179..2321
                     /locus_tag="PK976_02105"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HRC66712.1"
                     /translation="MAKLKILVVDDEPFIRSGIHRILDNFQVDYPFMTEPYEFEILDA
                     VTGEEAIEIINNHQPDIVLLDNKLPGIQGVEVLSYVKNKKLDIVVIMITSYASLDLAV
                     RATKDGAHDFVPKPFTPQELRSVIESTTKHIFLKRMTQQMNIAGKQIRFQFLSVLSHE
                     LKAPLNAIEGYLQMMKEKQLGDKIDDYAQVIDRSLARIKGMRTLILDLLDMTRIETGE
                     KEKKIERLNICAIAQTSIDTSKPMAIQRDVKIKLDCPKDLEFEADASDMEIIFNNLIS
                     NAVKYNKEGGSVTVRIHKDENNLVIEVSDTGIGMTEEETSRLFQEFVRIKNEKTRNIT
                     GSGLGLSIVKKIVDTYNGNISVQSTPDVGSTFIVTLPVQSLTDIIL"
     gene            2339..>2839
                     /locus_tag="PK976_02110"
     CDS             2339..>2839
                     /locus_tag="PK976_02110"
                     /inference="COORDINATES: protein motif:HMM:NF003995.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HRC66713.1"
                     /translation="MSHRSLPDKTIERLSQYRRNLLICLSQGKHHIFSHEIALIHHIT
                     PVQVRRDLMLIGYSGTLRKGYDVKELIDLIGLILDTEEGLKVAIVGIGNLGRSLIGYF
                     SGKRSKLNIVAGFDNNPDKIGKIYAGVHCYSSERMQEIIVEEEISIAIITVPADNAAI
                     VAERLVM"
BASE COUNT          938 a          540 c          581 g          780 t
ORIGIN      
        1 aatgacctga acatcaccaa cgaaaaactt gctcatgcca aagcggcttt aaaacaatcg
       61 gaaaaattgg cacacatggg tcagctttct gccggtattg cccatgaact caataatcct
      121 ttgggagtta ttacaatgta tgctaatatt atgatggaag aacggcctga gaatgatccc
      181 ctgcgtaagg atctggaatt gattgtggag caagccaatc gctgtaagaa aattgtaagt
      241 ggtttactca attttgcacg taaaaatcaa gtcaatctga ctgagaccca tctggaacaa
      301 ttagttcaac gaagcctcga ttcggtggtt aaacccgata atgtgaaaac aagaatgatt
      361 tcagaaatga aagaccctat ctgtttcctt gatcaagaac agatgatgca ggttttgaca
      421 aatctcgaaa aaaatgcgat tgaagctatg cctcccgaag gcggcgaaat tactgtcacc
      481 cttaaagaca atcttcatga agttattatt aaagttgccg atacaggcat aggcatcgag
      541 aaggaaaata tggacaagct atttactccc ttctacacta ccaaagagct gggcaaaggc
      601 actggcttgg gacttccgct catctatgga attgtgaaaa tgcacaaagg ccagattagc
      661 gtcatgtcga ataacaataa agaagaaggg cctacaggaa caatttttac aattacctta
      721 ccccgcaacc ccaattcagt ttaacaattt cattataaaa aaattaccat catggaagct
      781 cataagaaaa cgatttttat tgtcgatgat gatcccgatt acttgttcca gttgcaggta
      841 ttgctcgaaa agaatggatt tcgggtaatt acggccgaaa gccagcgaga agctcaaaaa
      901 atcatagaac gcaccaaacc cgatctggcc atagttgatt tgatgatgga aaatgaagat
      961 agtggtttta ttctgagcta tcaaatcaaa agattgtatc ccgatgttcc tgtaattatt
     1021 tttactgctg ttactgccga aacaggcata agttttgaaa cagaactggg catgaattct
     1081 cgatggatca aggccgacct gtttttacca aaaggtctgc gtcccgacca attgattcgt
     1141 gaaatcaata aattgttaaa ataataaaat attcaaaaat ggcaaaactc aagattttgg
     1201 tagtagatga tgagcctttc attcgctcag gcatacatcg catactcgat aatttccaag
     1261 tggattatcc ctttatgacc gaaccttatg aattcgagat actcgacgct gttactggcg
     1321 aagaagctat cgaaatcatc aataaccatc agcccgatat tgtcttgctc gataataaac
     1381 tccccggtat ccaaggtgtg gaagtgcttt cgtatgttaa gaacaaaaaa ctcgacatag
     1441 tagtcattat gattacttcc tatgcctccc tcgatctggc cgtcagagca actaaagatg
     1501 gggcacacga cttcgttcca aagcctttta ctcctcaaga attgagatcg gtgatagaat
     1561 ctacgacaaa acatattttt ctcaaacgta tgacccagca aatgaatatt gccgggaaac
     1621 aaatccgttt ccaatttcta tccgtcttga gtcatgaatt aaaagctcct ttgaatgcca
     1681 tagaaggata tctgcaaatg atgaaggaga aacagcttgg tgataaaatt gacgactacg
     1741 ctcaagtaat cgaccgttcg ttagcacgta ttaaaggcat gcgtacactt attttagact
     1801 tgctcgatat gacgcgcatc gaaacgggtg aaaaagaaaa gaaaatcgaa agactcaata
     1861 tttgtgctat agctcaaact tctattgata cttcaaaacc tatggctata cagcgtgatg
     1921 tgaagataaa gctcgattgt cctaaagacc tggaatttga agccgatgcc tccgacatgg
     1981 aaattatttt taataatttg atatcaaacg cggtaaaata caataaggaa ggtggaagtg
     2041 ttactgttag aatacataag gatgaaaata atttagtgat cgaagtatcg gataccggaa
     2101 taggtatgac cgaagaagaa acctctcgtc tgtttcagga atttgtaagg ataaaaaatg
     2161 aaaagacaag aaatatcaca ggcagtggtt tgggattatc tattgtaaag aagattgtcg
     2221 atacatataa tggaaatata tccgtgcaaa gtacacctga cgtggggagt actttcatcg
     2281 taaccctgcc tgttcagtcc ttaaccgata tcattctata aatcatttat ctatgaagat
     2341 gagccacagg tctttacccg ataaaaccat tgaaagattg agccaatacc gacgaaatct
     2401 tcttatatgc ttatcgcagg ggaagcatca tattttttca catgagattg ctctgattca
     2461 tcatattaca ccggtacaag tgcgtcgtga ccttatgctc ataggctatt ccggcacgtt
     2521 aagaaaaggt tatgatgtaa aggaattgat agatctgatt ggtttgatcc tcgatacgga
     2581 agaaggatta aaagttgcca tagttggaat tgggaatctt gggcgttctc tcattggcta
     2641 ttttagtggt aagagatcaa aactcaatat cgtggctgga ttcgacaata accccgataa
     2701 gatcgggaaa atctacgctg gtgttcattg ttattccagc gaaagaatgc aggagatcat
     2761 cgttgaggaa gaaattagca tagctattat tactgttccg gctgataatg ccgctatcgt
     2821 agccgaacga ctcgttatg
//
LOCUS       DAORPD010000121         2801 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_6407_length_2801_cov_3.096140, whole genome shotgun sequence.
ACCESSION   DAORPD010000121 DAORPD010000000
VERSION     DAORPD010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6407_length_2801_cov_3.096140"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            517..1500
                     /locus_tag="PK976_02115"
     CDS             517..1500
                     /locus_tag="PK976_02115"
                     /inference="COORDINATES: protein motif:HMM:NF021537.2"
                     /note="predicted Zn-dependent protease with a strongly
                     conserved HExxH motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing putative Zn-dependent
                     protease"
                     /protein_id="HRC66714.1"
                     /translation="MPKFLKIIVFLYCLLFGLQSFSQIKPAENSAFTVTTKDIDNFWV
                     AFDMLKNCNSSLDSIYCLKEYLFDNGTDGFKEFIRIYDYTSEDYLEAIRNYPKFFNSV
                     RANTLLTKEIYPEIIQLFQKIEKYYPNYIPLKICFVISPLRAGGTSTREFLFIGAEII
                     TSSNKVDLSEFGLNALGKILDSDTNIDKRLIYVIAHETIHHLQVNARLDNYDLLNRSL
                     FEGAADFIAWLLTGVRGNAYLYDYGISHEKELWMKFKKDIEAGVNTDNWMYNFDRVEN
                     DLPPDLGYFMGYRIVEAYFNKCLDKHQAVLDIIEMKNPEEFFKKSGYNPDS"
     gene            1914..2342
                     /locus_tag="PK976_02120"
     CDS             1914..2342
                     /locus_tag="PK976_02120"
                     /inference="COORDINATES: protein motif:HMM:NF015004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HRC66715.1"
                     /translation="MRIWSIHPKYLDSKGIIALWKEALLALIVLMGNTRGYRNHPQLL
                     RLRQLRKPLKSIDFYLNVVWQEAQNRGYNFDVSKFKQTETMTRIAITEGQVEFQLHHL
                     LNKLKKRDVKKYNEVKIIDMPVTHPLFYLVAGEVESWEKI"
     gene            2346..>2801
                     /locus_tag="PK976_02125"
     CDS             2346..>2801
                     /locus_tag="PK976_02125"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HRC66716.1"
                     /translation="MNKEFYLQTCYKISELITKRYSTSFSLAVSFLEKEQRSAIYAIY
                     GFVRLADEIVDSFHPHDKIFLLNKLTEDLYYSLGHNISTNTVLMAFADTVNKYKIPHS
                     HINAFLESMKRDLNITQYHKQEDLSQYIYGSADVVGLMCLRVFCHNEPSL"
BASE COUNT         1025 a          455 c          468 g          853 t
ORIGIN      
        1 aaaatacaat agatttaaca agcgaatatc gcgcgaagct tcataaaaat ttttaacatt
       61 ttaaaatgag gaaaattatc ctgaaaacca accgtttaac ttggcatatc gatgacacat
      121 gaatcgggtt ttatgaagat caggccctat ttagcaaatt gaaaacatcg agataagcat
      181 tgagtaaaaa cgaggatttt taaaaagcat atcgctttac accctgatgt ttagcgtcga
      241 aattccaatt tacgtcgaga taaaaaagta aaaggcaaca gggcaaagca caaataaatc
      301 aatcaaattc aactctatat ttattaatgg aagaaattta ttattatcac tcacaatttg
      361 aatgaaatat catttcgacg gaagcgttat aagaagaaga ttcgtacata gtattttcga
      421 aattatataa tttcgaaatc tatggatata taggaggttt aatagctaaa aaactctgct
      481 tgaattagtt gcgacaaatt aaaaattcta tgcatcatgc caaaattctt gaaaattatt
      541 gtttttttat attgtttatt atttggttta caatcttttt ctcagattaa acctgctgaa
      601 aatagcgctt ttactgtcac tacaaaagat atcgacaact tttgggtggc attcgatatg
      661 ttgaaaaact gtaattcatc attggatagc atttactgct tgaaagaata tctattcgat
      721 aatggaaccg atggttttaa ggaattcatt cgcatttatg attatacatc cgaagattat
      781 ttagaagcca tccgtaatta tcccaaattt ttcaacagtg ttagggcaaa tactctgcta
      841 actaaagaaa tctacccaga aatcatacaa ttattccaga aaatcgaaaa atattacccg
      901 aattatatcc cgctaaaaat ttgttttgtc atcagtccgc taagagctgg aggtacatcg
      961 accagggaat ttttatttat tggagccgaa ataattacat ccagcaacaa ggtcgatcta
     1021 tcagaatttg gattaaatgc tcttgggaaa attcttgatt ccgacaccaa tatagacaag
     1081 aggctcatat atgtgattgc ccacgaaaca attcatcatt tgcaagtaaa tgctcggctc
     1141 gataattatg atctattgaa tagaagtctt ttcgaaggtg cagcagattt cattgcatgg
     1201 ttgctaaccg gagtgagagg gaacgcctat ttatatgatt atggcatttc tcatgaaaaa
     1261 gaactctgga tgaaattcaa gaaagatatc gaagcaggtg taaataccga taattggatg
     1321 tataatttcg atagagtaga aaatgaccta ccacccgatt tgggctattt tatgggatac
     1381 agaatagtag aagcatattt caataaatgt ctggataaac atcaggcggt attggatatc
     1441 atcgaaatga aaaatccaga agaatttttc aaaaaaagtg gatacaatcc cgattcctaa
     1501 atttatcagt tatttgcgca tacttatcat cccattatct catcgatttg ctctaaaagt
     1561 ttaaattata aaaattaagg tttcaaatca taaaaaataa aatcatcaca accctaaagg
     1621 ttcaaatgac tgaataaaag tcagcaacat atacttgcaa caataataaa catcgaatat
     1681 ttcatcgaaa tgaacaggca aaatcatcaa aataaccttt gattgtatag tttggacaat
     1741 ttttttatat ttgcctaaat acttctatcc atgttgatca ataagcagaa caatatacct
     1801 acaggataaa atcatgaagg aatatataat tgcatcagcc atttgatctt acaagctaaa
     1861 tttttttaaa gaagtagaaa gaatatttta aattggattc aaaccatgaa aaaatgagaa
     1921 tttggtcaat acatcctaaa tatttggatt cgaagggaat aattgcatta tggaaagaag
     1981 ccttattagc cctaattgtg ctaatgggaa acacccgagg ctataggaat catccacaat
     2041 tgttgcgttt aagacagcta aggaagccat taaaaagtat tgacttctat ttaaatgtcg
     2101 tatggcaaga agcccaaaat agagggtata acttcgatgt atctaaattc aaacaaacgg
     2161 aaacaatgac tagaatagca attacggaag gacaagtaga atttcaacta caccaccttt
     2221 taaacaaact taaaaagcga gatgttaaaa aatacaatga agttaaaatt attgacatgc
     2281 ctgtaactca tccattgttt tatttagtgg ctggagaagt ggaatcatgg gaaaagatat
     2341 aagttatgaa taaagaattt tacttacaga cttgttacaa gatttcggag ctgatcacca
     2401 aaagatatag cacctccttt tcattagccg tgtcatttct cgaaaaggaa cagcgttcgg
     2461 caatttatgc catttatgga tttgttcgcc ttgctgatga gatcgtagat agtttccatc
     2521 cacacgacaa gatttttctt ttgaacaaac ttaccgaaga tctttattac tcgctcggtc
     2581 acaacatcag cactaacaca gttctcatgg catttgctga taccgtaaat aaatacaaaa
     2641 ttcctcacag ccatatcaat gcttttctcg aaagcatgaa gcgagattta aatattactc
     2701 aataccacaa gcaggaagat ttgagtcaat atatctatgg ttcggccgat gttgtgggat
     2761 tgatgtgcct aagggtattt tgtcataatg aaccgagcct t
//
LOCUS       DAORPD010000122         2801 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_6408_length_2801_cov_2.407502, whole genome shotgun sequence.
ACCESSION   DAORPD010000122 DAORPD010000000
VERSION     DAORPD010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6408_length_2801_cov_2.407502"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /gene="smpB"
                     /locus_tag="PK976_02130"
     CDS             <1..399
                     /gene="smpB"
                     /locus_tag="PK976_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HRC66717.1"
                     /translation="LIETYVAGIQLTGTEIKSIRAGKVNLTDAYCSFIGNELFVLNMH
                     ISEYELGTHYNHDPKRHRKLLLTKRELKRLQTKVKEKGFTIIPIRLFINEKGYAKLEI
                     ALAKGKHSYDKRESIKQKDQKRDMERFENY"
     gene            523..1344
                     /locus_tag="PK976_02135"
     CDS             523..1344
                     /locus_tag="PK976_02135"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: protein motif:HMM:NF003742.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HRC66718.1"
                     /translation="MRKIYILSDGTGRTASYALEAAMVQFQEMKDETKIELFPDVRTS
                     QQIDQIVEKASKEEALIIYSLVGEQTRHYMQTICRHYIIETVDLMGPLLMKLSAHFER
                     QPAGTPGLYQQMNKEYFRRIDAVQFAFTHDDGLRIEELSKAEIVLVGVSRSFKTPLSI
                     YLAYKGWFVANVPIVLDIEPPEELFRIPPHRVFALTTSALELSKLRQTRVEILNGYAE
                     NYASFDYVKKELAWAHTIYRKQPLWHIIHISHKSIEEISIEILSFIRQHHNNHPF"
     gene            complement(1644..>2801)
                     /locus_tag="PK976_02140"
     CDS             complement(1644..>2801)
                     /locus_tag="PK976_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRC66719.1"
                     /translation="DLQAVQLKATPNQDGKTWRLNGVKRFITNGDADISLVLARSEEN
                     TTDARGLSLFLYDRNSKAVTIRRIENKLGIKGSPTCELVFRDAPALLVGERKLGLIKY
                     VIALMNSARLGVGAQSVGIAEAAYREALKYANEREQFGKAIVNYPAIYEMLIRMKVKI
                     DAMRSLLYETARFVDINKSYNIAAEKRKLEPNERNEQKYYQKMADMYTPLLKLISSEY
                     CNQIVYDALQIHGGPGFMKDFPVERLYRDARITSIYEGTSQLQVVAAIRGVTTGFYLK
                     AIQDYDSQIIIPEEYLKKSLQLMTQQYKETVDKVNTEKDSEIVEFHSRRLVEMAGNII
                     LGYLLLLDSQRNPEFLKSARIFIENAKSENQSMHEYIMNFNDREIDNFKFI"
BASE COUNT          847 a          581 c          542 g          831 t
ORIGIN      
        1 ctgatagaaa cctatgtggc tggtatacag cttacaggaa cggaaatcaa atccatcaga
       61 gctggcaagg tcaatctcac cgatgcttat tgtagcttca taggaaatga attatttgtt
      121 ctgaacatgc acatttccga atatgaatta ggtacacatt ataatcacga tcctaaacgc
      181 catagaaaat tattgctgac aaaaagagaa ctcaaacgct tgcaaaccaa ggtaaaagag
      241 aaaggtttta ccatcattcc gatacgttta ttcatcaatg aaaaaggtta tgcaaaactc
      301 gaaattgctc tggccaaggg aaaacatagc tatgacaaac gagaaagcat taaacaaaaa
      361 gaccagaaac gcgatatgga acgcttcgag aattattgat ttgtattgtt tttctctaaa
      421 cacaataccc attctcaaaa aatctaattt ctctgctttg aatcggtaaa aatctcgaaa
      481 attatcggat atttgaggat aaaccaataa tattagacct ctatgagaaa gatatacatc
      541 ttgtcggatg ggactggccg cacggcgagt tatgccttgg aagctgccat ggttcagttt
      601 caggaaatga aagatgagac aaagattgaa ttatttccgg atgtaaggac ttcgcagcag
      661 atcgaccaga ttgtggaaaa ggcatcaaaa gaggaagctc ttattattta tagtcttgtg
      721 ggagaacaaa cccgacatta catgcaaact atctgcaggc attatatcat agaaacagtt
      781 gatctgatgg ggcctctact tatgaaactc tcggctcatt tcgagcgtca gccagctggc
      841 acaccaggac tctatcaaca aatgaataaa gaatatttta gaagaatcga tgctgtgcag
      901 tttgctttta cgcatgatga cggtttgcgt atcgaggaat tgtcgaaagc cgaaattgtg
      961 cttgttggcg tcagccgctc attcaagacc cctttgagta tttatctggc gtacaaaggc
     1021 tggtttgttg ccaatgtccc tatcgttctc gatatagaac cacccgaaga actgttccgt
     1081 attcctccac atcgtgtttt cgcattgacc acttccgctc tcgagttgag caaattacgt
     1141 cagacacgtg tggaaattct aaatggatat gccgaaaact atgcctcgtt cgattatgtg
     1201 aaaaaggaat tggcatgggc gcacactatc tatcgcaaac agcccttgtg gcatattatt
     1261 catatcagcc ataaatctat cgaagaaatt tcgatagaaa tcctgtcttt tatcaggcaa
     1321 caccataaca accatccctt ttaacttcag actcttctga aaaacttcta tcccttacat
     1381 cacaaaagct atccccagag taaagctact gaaaattcgg caataaatca aagtatcgaa
     1441 atcaaacttg tttacaaatc gcaagacatt tgcaaatgtc aacatattat tgtccgtttt
     1501 taaaatcata atacaaatga aaaggagtgg tcggtcgtaa caaagttttc aaaggaacag
     1561 aacctctgaa ctctcagcct tgctgcgaca ttcattgcat atcgtttctg taaagatata
     1621 ttgcccttac tggaaaactt ttgctaaatg aatttgaaat tatctatctc gcgatcgtta
     1681 aaattcatga tatattcatg catgctttgg ttttctgatt tggcattttc gatgaaaatc
     1741 cgagcagatt tcaagaattc aggatttcgc tgagaatcga gcaaaaggag ataacccaag
     1801 ataatgtttc ctgccatttc taccaatctg cgggagtgaa attcaacaat ttcactatct
     1861 ttttcagtat taaccttgtc aactgtttct ttatattgtt gggtcatcaa ttgaagcgac
     1921 tttttcaaat actcttctgg aatgattatc tgcgaatcat aatcttgtat tgctttaaga
     1981 taaaatccag ttgtaacgcc tctaatggcg gctactacct gtaactgtga tgttccttca
     2041 taaatgctag taattctggc atcccggtat aggcgctcta cggggaaatc cttcataaat
     2101 ccaggtccac catgtatctg gagagcatca taaacgatct ggttacagta ttcgcttgaa
     2161 ataagcttca atagaggtgt gtacatatcg gccattttct gataatactt ttgctcattg
     2221 cgctcgttag gctcgagttt tcttttttcg gcagctatgt tatatgattt gttaatatcg
     2281 acaaatcggg cggtttcgta aagtaaggag cgcatggcat cgatttttac cttcatgcgt
     2341 ataagcattt cgtatattgc tgggtaattg acgattgcct tgccaaattg ttcgcgttcg
     2401 ttggcatatt tcaatgcttc gcggtaagct gcctcggcta tgcctacaga ttgggctccc
     2461 acacccagac gagccgaatt catcagggca attacgtatt tgataaggcc caatttcctc
     2521 tcccctacca ataaagctgg agcatctcga aatacgagct cacaggtagg tgaacctttt
     2581 atcccaagtt tgttctcgat tctgcgaatg gtaactgctt tcgaatttct atcatacaaa
     2641 aacaaggaaa ggcctctggc atcggtagta ttctcttccg agcgagccaa aaccagagat
     2701 atgtcggcat ctccattggt aataaagcgt ttcaccccat tcaatcgcca agttttacca
     2761 tcctgattgg gcgttgcctt gagctggact gcctgaagat c
//
LOCUS       DAORPD010000123         2799 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_6415_length_2799_cov_1.637391, whole genome shotgun sequence.
ACCESSION   DAORPD010000123 DAORPD010000000
VERSION     DAORPD010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6415_length_2799_cov_1.637391"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..1606)
                     /locus_tag="PK976_02145"
     CDS             complement(110..1606)
                     /locus_tag="PK976_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HRC66720.1"
                     /translation="MKININQFKHIGSGLLLIATLSLLPISCTKDFLDEELKGEFSSQ
                     TFYQNDKQALQALTGVYNAIAFTSFNNAIWVFGDVASDDAVKGGNPGDQAEITYIDEF
                     TVDRNNGIINNYWTFLYEGVSRANNVINKVPGVKMDETLKQRIIGEAKFIRAYNYFLL
                     VNIFGKVPLKLLPQDTPEAINVPLSEISTIYQQIQTDLTEAAAVLPVSYGSSEKGRVS
                     KGAALAMLGKAALYQQQWALAIDYFHQLEALGIYRLETNYANLFKLSHENGVESIFEI
                     QHLSDQIPFIGNVLNQWFAPASEGGYYFNAPTTSLVNEFEKASNQTPDPRLDISIGRD
                     GIPWLNGEPFQASWSPTGYLTKKHQQPLSEVPIAVKGDGGLNYIYMRYADVLLMKAEA
                     FNELNQTDSALANLNKVRARARNSFTSGNPPADLLPNVTTTDQHLLRNAIRHERRVEL
                     AQEFHRYFDLMRWGKQTAEEALGSGFSYENNRYFPIPQNEIDANEAIP"
     gene            complement(1621..>2799)
                     /locus_tag="PK976_02150"
     CDS             complement(1621..>2799)
                     /locus_tag="PK976_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRC66721.1"
                     /translation="RLGYGAIGNQEIGLYAYSDRISPYYNYPFGEIVSSGYAQTALGN
                     EKLKWETSYQYNAGIDAEFWKGALAISLDYFYKITDDMLVKASYPPSAGYASSPWINN
                     GSVLNTGLELEASYRKAGNDWSYNIGGNLTYLHNEVRKLNAPLIGGRVESGVYATYTT
                     EGQPIGAFYLLEMDGIFQNETEIILSAFQGNNIHPGDVKFVDQNKDGKITNEDRVFLG
                     SAIPKFSGGISFNGQWRQWDMGIFLQGVAGNKIYIQINQDIEGFYRGFPVTQRYFDNR
                     WTGEGTSNTQPRPSWTAKSNNARPSSRFLEDGSYLRLKNIQIGYTLPKETLNFLGFTK
                     ARIYLSASNLFTITSYPGLDPEMTVSDNSKNEGDRASGIDWGTYPSTMSIMLGLDLSI
                     "
BASE COUNT          767 a          572 c          609 g          851 t
ORIGIN      
        1 tttttgtgta attgatttta aagcaaaatc atctaaaata cttgcttttt taaacatagg
       61 tcttgagttt taaaaggtta ataataaatt aattatttaa taataaaggt tacggaattg
      121 cttcattagc atctatttca ttctgtggaa tggggaaata acggttattt tcgtatgaaa
      181 atccactgcc aagagcttct tctgcggttt gctttcccca gcgcatgagg tcgaaatagc
      241 gatgaaactc ttgggcgagt tctactctgc gttcatggcg aatggcgtta cgaagcagat
      301 gttgatcggt agtagtcaca ttgggaagaa gatcggcagg aggattgccg ctggtaaaac
      361 tgttccgcgc tcttgcacgt accttgttca ggttagccag agccgaatcg gtttgattca
      421 attcattgaa agcctcggct ttcatcaata aaacatcggc atatctcatg taaatgtaat
      481 taaggccacc atcgcctttt accgcaatag gaacttccga cagaggttgc tgatgttttt
      541 tggttaaata acccgtggga gaccatgaag cctggaatgg ttctccgtta agccatggaa
      601 ttccatcgcg gccaatactt atgtcgagcc ttggatcggg agtttgattg gaagccttct
      661 cgaattcatt caccagcgaa gtggtaggcg cattgaaata ataacctcct tctgatgcag
      721 gtgcaaacca ttgattgaga acatttccga taaaaggtat ctggtcagat agatgttgta
      781 tctcaaaaat ggattctaca ccattttcgt gggatagttt gaaaagattg gcataattgg
      841 tctcgagtct ataaatccct aaggcctcga gttgatggaa ataatctatt gccagagccc
      901 actgttgttg atacagtgct gccttgccta acatggccag agcggctccc ttgcttactc
      961 tgcctttctc ggaactgcca tacgaaacag gcagaacggc tgctgcctca gttaaatcgg
     1021 tctgaatttg ctgataaata gtactaatct ctgacagagg tacattgatg gcttcaggag
     1081 tatcctgagg caaaagtttt aagggtacct ttccaaagat gttgaccagt aagaaataat
     1141 tataagccct gatgaactta gcttcgccga tgatacgttg tttgagggtt tcatccattt
     1201 ttacgccggg tactttattg attacattat tagctctcga taccccttcg tataaaaatg
     1261 tccagtaatt attgatgatg ccgttattac ggtcgacggt aaattcgtct atataggtaa
     1321 tttctgcctg atcgccagga ttgcctcctt ttacggcatc atccgaggcc acatctccaa
     1381 agacccatat cgcattattg aagctggtaa aggcaatggc attgtaaaca ccggtgagag
     1441 cctgcaaggc ttgtttgtca ttctgataga atgtctggct gctaaattct cctttcagtt
     1501 cttcatcaag aaaatctttt gtgcaggata tgggaagaag gctcaaggtg gctattaaca
     1561 gaagtccgct accgatatgt ttgaattgat ttatattgat tttcataata tttcaatttt
     1621 ttagatagaa aggtctaaac ctaacataat actcattgtc gaaggataag ttccccaatc
     1681 tataccactg gctctgtcac cttcattttt cgagttgtcg ctgacggtca tttccgggtc
     1741 gaggccagga taggaggtaa tggtgaaaag attgcttgcc gaaagataga ttcttgcttt
     1801 ggtaaagcct aagaagttaa gtgtttcttt gggaagggtg tatccaatct gaatattttt
     1861 caatcggaga taggaaccgt cttcgaggaa acgtgaggaa ggtcgtgcat tattcgattt
     1921 tgcagtccat gacggacggg gttgagtatt cgaagtcccc tcgcccgtcc agcgattgtc
     1981 gaaataacgc tgagtaacag ggaagccacg ataaaagccc tcgatatcct gattgatttg
     2041 aatgtatatt ttgtttccgg ctacgccctg cagaaatatt cccatgtccc attgacgcca
     2101 ttggccatta aagctaatgc cgccactgaa tttgggaata gcactgccta aaaatactct
     2161 gtcttcgttg gtaatctttc catctttatt ctgatctaca aacttcacat caccggggtg
     2221 gatattgttt ccttgaaaag ccgaaagaat tatctcggtt tcattttgga aaataccgtc
     2281 catttcgaga agataaaaag ctccaatggg ttgtccttca gtagtatagg tagcgtatac
     2341 acccgattcg actctaccac caatcagagg ggcattcagt tttcttactt cattatgcaa
     2401 ataagtaaga ttaccgccta tattgtagct ccaatcgttg ccggctttgc gataactggc
     2461 ttcgagttcg aggccggtgt tcaataccga accattatta atccaagggg aagaggcata
     2521 acccgccgaa ggaggataag acgcctttac aagcatatca tcggtaattt tatagaaata
     2581 atccagtgaa attgccagag cacctttcca gaattcggca tctatgccag cattatattg
     2641 ataactcgtt tcccatttca gtttttcatt tcccagagcg gtttgagcat agccgctact
     2701 tacgatttca ccgaaaggat aattataata gggtgatatc ctgtcggaat aggcataaag
     2761 gccgatttct tgattgccta tggctccata gcccagcct
//
LOCUS       DAORPD010000124         2795 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_6429_length_2795_cov_1.927737, whole genome shotgun sequence.
ACCESSION   DAORPD010000124 DAORPD010000000
VERSION     DAORPD010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6429_length_2795_cov_1.927737"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="PK976_02155"
     CDS             <1..602
                     /locus_tag="PK976_02155"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRC66722.1"
                     /translation="FCGSGTLLTEAALIAHNLPAGLFRKSFGFMHWRDFDRTLWNDIK
                     ADARKHKRNQECEIFGSDVSEEAIAIASRNIHRAGFSSEITLKKTRFEDTSFDGPGVL
                     ITNPPYGERLQTNDIVALYKSIGDTLKHNYKGFDAWIITSDFFALKNLGLHPTKKVIL
                     YNGPLECRFAHFEIYEGSRKQKNDLFNTLETLPDTSIEE"
     gene            607..>2795
                     /gene="priA"
                     /locus_tag="PK976_02160"
     CDS             607..>2795
                     /gene="priA"
                     /locus_tag="PK976_02160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HRC66723.1"
                     /translation="MKQRATYFADILLPLAVEGYFTYRIPNDLAKDIKLGMRVVVQFG
                     KQKFYTGLVRRIHQNIPKDYTPKYVLSIVDPYPLVSEVHFQFWEWLASYYMANVGEVM
                     NVALPSSLKLASETRLALHPDFDKDYSGLTEKEMMVAEACEIQKQITLADVARITEMA
                     SVFGLIKKLIDKKVIVLEEELQNRFQPKTRIYLSLHPDYKDNDEKLNKIFNELEKRAP
                     RQSEALLMFLSMTRDREEISRNSLQAMANLGPSPVDGLIKKGIFVSKEHVVSRLGELY
                     KGETQEVRLTPLQQKVYESIRKQWSEKPTVLLYGITSSGKTEIYIKLATEIIQQGGQV
                     LYLLPEIALTAQIVNRLRRFFGDDVAVYHSKFNENERAEIWNRVTENPLYDLKPFKLV
                     VGTRSALFLPFSNLKLIIVDEEHDASFRQAQPAPRYNARDAAIYLAGLLHARTLLGSA
                     TPSLESYFNAQSGKYGFAQILTRYSDIMMPEIILADLREEKNKGASKSLFSQTLLQLI
                     SEALSQKEQIILFQNRRGYAPRIECGKCNWIPLCKNCDVSLVYHKEYHQLQCHYCGYT
                     APLPTECSVCHHTALYLRGFGTEKIEDELSIYYPEARIARFDLDSTRNKNAHVDLIQR
                     FEDRDIDILVGTQMVTKGLDFDHVSLVAILNADNMLAYPDFRAFERGYQLMAQVAGRA
                     GRKNKRGKVVIQTYNPQHPAILFVLSNDYEGMAHQQLSERAHYHYPP"
BASE COUNT          851 a          674 c          560 g          710 t
ORIGIN      
        1 ccttttgtgg tagcggaacg ctattaaccg aagctgcact tattgctcat aacttgcccg
       61 caggactttt cagaaaaagt ttcggattca tgcattggcg cgacttcgac cgaacactct
      121 ggaacgatat caaggcagat gcccgtaagc ataaacgaaa tcaggaatgt gaaatcttcg
      181 gcagcgatgt ctccgaagaa gctatcgcga tagcttcgag aaacatacac cgtgctggat
      241 tttccagcga gattaccttg aaaaaaacac gtttcgaaga tacttccttc gatggccccg
      301 gcgttctgat taccaatcct ccttatggcg agagattgca aaccaatgat atcgtcgccc
      361 tgtacaaaag cataggcgat actctcaaac acaactataa aggcttcgac gcctggatca
      421 ttacttccga cttctttgca ttgaaaaatc tgggcttaca ccctaccaaa aaagttatcc
      481 tctataatgg gcctctcgaa tgccgctttg ctcacttcga aatttacgag ggaagcagaa
      541 aacaaaaaaa cgaccttttc aatactttag aaactcttcc cgatacctcc atcgaggaat
      601 gaaaacatga aacaacgtgc cacatatttt gccgatattt tacttcccct ggccgtcgag
      661 ggatatttta cttaccgcat ccccaacgac ctcgccaagg atattaaatt aggaatgagg
      721 gtggtagttc agttcggtaa acagaaattc tacaccggat tggtccgacg tattcatcaa
      781 aacatcccca aagattacac gccaaaatac gttctttcga tagtcgatcc ttatcccttg
      841 gtaagcgaag tgcattttca gttctgggaa tggttagctt cttattatat ggccaatgtg
      901 ggcgaagtga tgaatgtggc tctaccttcc tccttgaaac tggccagcga aacacgcctc
      961 gctctccacc ccgatttcga caaagattat agtggcctga ctgaaaaaga aatgatggtg
     1021 gccgaagcct gcgaaatcca aaaacaaatt acactggccg acgtggcacg aatcaccgaa
     1081 atggcctctg tattcggctt gatcaagaaa ttgatcgaca aaaaagtcat cgtactcgaa
     1141 gaagaactcc aaaaccgttt tcaacctaaa acaaggatct atctatcgct tcaccctgat
     1201 tacaaagaca atgacgaaaa attgaacaaa atcttcaatg aattagaaaa acgagcacct
     1261 cgtcagagtg aagccttgct catgtttctg agtatgacac gcgacaggga agaaattagc
     1321 cgcaacagcc ttcaggccat ggcgaatctt ggaccttctc ccgtagatgg cctcatcaaa
     1381 aaaggaatat tcgtgagcaa ggaacacgtg gtaagccgct tgggtgaact ttacaaaggt
     1441 gaaacacagg aagtaagatt aactcctctt cagcagaaag tctatgaatc cattcgaaaa
     1501 caatggagcg aaaaaccaac ggttttactt tacggaataa cttcgagcgg caagaccgag
     1561 atttacatca aattagccac cgaaataatt cagcagggag gccaagtgct ttatctgctt
     1621 cccgaaattg ccctcacagc tcaaatagtc aaccgactgc gaagattctt tggcgacgat
     1681 gtagccgttt atcattccaa attcaatgaa aatgaaagag ccgaaatatg gaaccgggtt
     1741 actgaaaatc cactctatga cctaaaaccc tttaaacttg tcgtaggaac acgttcggct
     1801 ttgtttcttc ctttttcgaa cctgaaactc atcattgtgg acgaagaaca tgatgcttca
     1861 tttcgtcagg cacaacctgc accacgctac aacgcccggg acgccgccat ctatcttgcc
     1921 ggcttgctac atgcacgaac ccttctgggt tcggccactc cttcactcga aagctatttc
     1981 aatgctcaat cgggtaaata tggcttcgca caaattctta ctcgttatag cgacatcatg
     2041 atgcccgaaa tcatcttggc cgacctgaga gaagaaaaaa acaaaggagc ctcgaaatct
     2101 ttattttcac aaactttatt acaactaatc tccgaagccc tttctcaaaa ggaacaaatt
     2161 atcctgtttc agaaccgaag aggttatgcc cctcgcatcg aatgtggaaa atgcaactgg
     2221 attccgcttt gtaaaaactg cgatgtttct ctagtctatc ataaagaata tcatcaacta
     2281 caatgccatt actgtggtta tacggcacct ctgcccactg agtgctccgt ttgccaccat
     2341 accgcactct atttgagagg ttttggaaca gaaaaaatag aagacgaact ttccatctat
     2401 tatcccgagg cacgtattgc gcgtttcgat ctcgattcga cgagaaataa aaatgcacat
     2461 gtcgatctta ttcaacgttt cgaggacaga gacatcgata ttctggttgg tacgcaaatg
     2521 gtaaccaagg gactcgattt tgaccacgtt tcactagtag ccatcttaaa tgccgataat
     2581 atgctggctt atcccgactt cagagctttt gaacggggtt atcaattgat ggcacaggtg
     2641 gcaggccggg caggacgaaa aaacaaaaga ggaaaagttg ttattcaaac ctataatcct
     2701 cagcaccctg ccatactttt cgtcttgtcg aacgattacg agggaatggc tcatcagcaa
     2761 ttgagtgaac gagctcatta ccattatcct ccttt
//
LOCUS       DAORPD010000125         2787 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_6459_length_2787_cov_2.446559, whole genome shotgun sequence.
ACCESSION   DAORPD010000125 DAORPD010000000
VERSION     DAORPD010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6459_length_2787_cov_2.446559"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..913)
                     /locus_tag="PK976_02165"
     CDS             complement(<1..913)
                     /locus_tag="PK976_02165"
                     /inference="COORDINATES: protein motif:HMM:NF016827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-54 factor"
                     /protein_id="HRC66724.1"
                     /translation="MLHQKLQQKLLQKLSPQQILLMKLLQVPTLALEQRIKQELEENP
                     ALEEVDNEEVYDQEENNDFDADNLNADEVSESAEAESDEPIVDQGDEFDMEDYMSEDD
                     LDSYRYQVNNTSPDDEQHEMPLVSSTTFQENLLQQLSLKNISERTYQIASHLIGNLDD
                     SGYLNRDLESLVDDLAFLQNIQTNVRELENTLEIIHELDPPGIGARSLKECLLIQLRR
                     KPQSESVKNATTLLERYFDEFSKRHYDKILKKTHLTELELKQAIDEILKLNPKPGNIF
                     GDSFTAQQYVVPDFIIYNRDGELELTLN"
     gene            complement(1089..2477)
                     /gene="asnS"
                     /locus_tag="PK976_02170"
     CDS             complement(1089..2477)
                     /gene="asnS"
                     /locus_tag="PK976_02170"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019149647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HRC66725.1"
                     /translation="MQRTKIIDLLRSEEIGLKVRVMGWVRTRRGNKNIAFIALNDGST
                     IHHLQIVAELEKFDEETIKRITTGCALQVEGRLVESPAKGQPVELAAEKIEILGEANP
                     ESYPLQKKGHSLEFLRSIAHLRPRTNTFGAVLRIRHALAFAIHKFFNDKGFVYVNTPI
                     ITATDAEGAGEMFRVTTLDINHPPRKPDGGVDFSKDFFGRETNLTVSGQLEAELVALA
                     LSQVYTFGPTFRAENSNTPRHLAEFWMIEPEMAFYDINENMDLAEEFLKYLISYTLEH
                     CMDDLKFLNRMCDEDLINRLENVVQSKFERLTYTEAMTILESSGLKFEFPLGWGNDLQ
                     AEHERYLVEKHFGRPVILTDYPKNIKAFYMKQNHDGKTVRAMDVLFPRIGEIIGGSQR
                     EENLEALLDRIHELGMNEKELWWYIDSRRFGTVPHSGFGLGFERLVQFVTGMSNIRDV
                     IPFPRTPQNAEF"
BASE COUNT          761 a          571 c          571 g          884 t
ORIGIN      
        1 tatttagagt cagttctaac tctccatctc tattataaat gataaaatca ggaactacat
       61 attgttgggc agtgaaacta tcgccaaaga tgtttccagg tttggggttc agtttcaaga
      121 tttcatcgat ggcctgtttc agctcaagtt cagtcaggtg agtttttttc aaaatcttat
      181 catagtgtct tttgctgaat tcatcgaaat atcgttcgag aagagtagtt gcatttttca
      241 ctgattctga ctgaggtttt cggcgaagtt gaattaagag gcattcttta agcgaacgtg
      301 cgcctatgcc aggaggatcg agttcgtgaa tgatttccag agtgttttcg agttctctta
      361 cattggtttg aatattttgc aggaaagcca gatcgtcaac cagagattcg agatcgcgat
      421 tcaaatagcc cgaatcgtcg agattgccga ttagatggct ggcaatttga taggtgcgtt
      481 cagaaatatt tttgaggcta agttgctgaa gcagattttc ttgaaacgta gtggaagata
      541 ctagaggcat ttcatgctgc tcatcatcgg ggctggtgtt atttacctga taacggtatg
      601 agtcgaggtc atcctcgctc atgtaatctt ccatatcgaa ttcatcaccc tggtcaacaa
      661 tgggttcatc gctttcggct tctgcgcttt cagaaacttc gtcggcattc aggttatccg
      721 catcgaaatc gttgttttct tcttgatcat aaacctcttc attgtcaact tcttccaatg
      781 ccggattttc ttcaagttcc tgcttaattc tttgctcgag cgccaaagta ggaacctgca
      841 gcaatttcat cagtaaaatt tgctgaggcg aaagcttctg taagagtttc tgttgtaatt
      901 tctgatgaag cataattgat tatggcgctt atttatactt ttcgaattga aatttgttta
      961 tatcatttcg agtgggtcga ttcacttgat ttgaagcatc aacaaaatta atcatttttt
     1021 atttatggga ttgccataca ggaaataaat tagaaaaccc cagaaataaa agggattgcc
     1081 tctatttgtt agaattcggc attttggggt gtacggggga atggaatcac atcacgtatg
     1141 ttgctcattc ctgttacgaa ttgcacaagg cgttcgaaac caagtccaaa tccactgtgg
     1201 ggtacggttc caaatcgtcg cgaatcgatg taccaccaga gttctttctc attcattccg
     1261 agttcatgaa ttctatcgag cagagcctca agattttctt cacgctgtga acctccaata
     1321 atttcgccaa tacgtgggaa cagaacatcc atagcacgca cggttttgcc atcgtggttt
     1381 tgtttcatgt aaaaggcttt gatatttttc ggataatctg taaggataac cgggcggccg
     1441 aaatgtttct cgacgaggta acgttcgtgt tctgcctgta aatcattgcc ccagcctaat
     1501 ggaaattcga atttcagacc cgaactctcg agaatcgtca ttgcctcggt ataagttaaa
     1561 cgctcgaatt ttgactgaac cacattttct aaacggttaa tcaaatcttc atcacacatc
     1621 cgattcagaa atttcaagtc atccatgcaa tgttcgaggg tataggaaat caagtatttt
     1681 agaaattctt cggcgaggtc catattttca ttgatatcgt aaaaggccat ttcgggttcg
     1741 atcatccaga attctgccaa atgtcgtgga gtgttggaat tttcggctct gaaggtcggt
     1801 ccgaaggtat aaacctgtga cagggcgagg gctacaagtt ctgcttccaa ttgccccgaa
     1861 actgtcagat tggtttccct gccgaaaaaa tctttcgaaa aatcaacacc tccatccggt
     1921 tttcttggtg gatgattgat atcgagggta gtcactctga acatttcacc tgcgccttcg
     1981 gcatcggtag cagtaatgat gggagtattt acataaacga atcctttgtc gttgaaaaat
     2041 ttatggatgg caaatgcgag ggcatgacga atacgaagaa ctgctccaaa agtattggtg
     2101 cgggggcgta aatgagctat ggaacgtaaa aattccaaac tatgaccttt tttttgaagg
     2161 ggataagatt cgggattagc ttctcctaag atctcgattt tttcggccgc caattctacg
     2221 ggttggcctt tagccggcga ttctacaagc ctgccttcca cttgtaaagc acaacccgta
     2281 gtaatacgct ttatcgtttc ttcgtcaaat ttttccaatt ccgctactat ttgaagatga
     2341 tgaatagtag aaccatcatt caaagcaata aaggcaatat tcttatttcc gcgacgagtc
     2401 ctcacccaac ccatcactct tactttcaat ccaatctctt ccgatctcaa tagatcaata
     2461 attttcgttc tctgcatata aagggaatat tttacatctt tttatcgagt ttgcaaaatt
     2521 aagagcaatt tttgtaacga cagaaaagtg atcgatcctt tctcccataa tagaaataat
     2581 atcattgcct caaacgcaaa gctcaatgat cttgcaaaag aatcgatctg attgttgaat
     2641 tttagggcag agtgcctcca agtaagtaat gatgagcaag gatttcttcc tcctacatgt
     2701 aaaaggaatc atgtcattta ccgacggttt cgaagccgtt tcaaaggttt cccttcagct
     2761 tccccgactt tatcaacgcc cactttt
//
LOCUS       DAORPD010000126         2785 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_6462_length_2785_cov_2.956410, whole genome shotgun sequence.
ACCESSION   DAORPD010000126 DAORPD010000000
VERSION     DAORPD010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6462_length_2785_cov_2.956410"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            214..1311
                     /gene="wecB"
                     /locus_tag="PK976_02175"
     CDS             214..1311
                     /gene="wecB"
                     /locus_tag="PK976_02175"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HRC66726.1"
                     /translation="MKKVLSVVGARPNFIKIAPIHKAFREVESICEHKICHTGQHYDT
                     LMSDIFFDELDIPTPDFFLNAGGGTHAKQTATIMLRFEKILQQYQPDIVLVPGDVNST
                     IACSLTAVKLGIKVGHIEAGLRSFDRSMPEEINRIVTDALSDYLFVTENSGLRNLKYE
                     GAADEKIFFVGNTMIDNLIQNLEKISNKHFFSSLNLKLKEYIVTTFHRPSNVDSPDNL
                     NEMVNFLNEVAKKYDIVFPIHPRTLNNLKKFHLLKKINTNVHLIEPLGYINFLSLVID
                     SKGVVTDSGGIQEETCFLKIPCITVRNSTERPITVEMGTNHLVGSNFDAALNAIEVFN
                     NSLQNTMIPPLWDGKTSKRIVEIILHHLQPQ"
     gene            1248..2510
                     /locus_tag="PK976_02180"
     CDS             1248..2510
                     /locus_tag="PK976_02180"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRC66727.1"
                     /translation="MGWENFKKNSGNNFTSFAASMKIGMLLDNEFVSDPRVWNEALML
                     TQKGHEVHVICLNFGKQNKYELKNKIHIHRITIPRKIKNLLFITQLIFPGYKIFWYYF
                     SKKIIQKYNLRVIHAHDLYMALPAILLKQMYNIKIILDLHENFPAAVKAYTWAQKKHV
                     KWIFKASRWERIENGLLQKSDAVIVLSNYFKNTLIKKYNNLNSDKIYVYPNVPNIAEF
                     HSFKIDPYAYEFMTKGFWMVYFGVVAIRRGITYLLEAVDRISNPEIKLLIIGPIDKHD
                     RELFEPKFRKYEKEGKIIYYPWKDISELPSLLFHAKLGVSPILRNPQHESGVANKVFQ
                     YMLLEKAILVSDCKPQVDIIKEYHCGLYYQDSNIDDLIEKILWCYRNTDALTEMGRRG
                     RKAVIEKYNSDVQFQPIIEFYNNLESFG"
     gene            2503..>2785
                     /locus_tag="PK976_02185"
     CDS             2503..>2785
                     /locus_tag="PK976_02185"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRC66728.1"
                     /translation="MDNKIIYENYNWGSLGTNKLGPKIKKIIENIPSDVQSIIDIGCG
                     NGVITNELAKKYKVLGVDRSKSALQYVTSEKLEASCDNIPLPSQNFDMVF"
BASE COUNT          999 a          415 c          486 g          885 t
ORIGIN      
        1 attttgatga ctgtatggac agactctaat taagtctcaa agatacacaa tatttttcag
       61 aatggatata tctggagagc attttttcta aatgtcttcc taactttgca tagaatttaa
      121 taaaaacgag atggctaagc gttcggaaaa tttttttagg atttgtcagg aaagaataaa
      181 aatttataac tctgcaaact caaaatttcg aaaatgaaaa aagtactttc tgttgtaggt
      241 gccagaccta attttataaa aattgctcca attcataagg cttttcgaga agttgaatcc
      301 atttgtgaac ataagatctg ccataccggt cagcattacg atacattgat gtcggacata
      361 ttctttgatg aactagatat tccaacacct gactttttcc tgaatgctgg agggggaact
      421 catgctaaac aaacagctac aattatgttg cgttttgaaa aaattcttca acaatatcaa
      481 cctgatatag tattagtacc cggcgacgta aattcaacaa tagcatgttc cttaaccgca
      541 gtgaaactcg gaattaaagt cggccacatc gaggccggtt tgagaagttt tgaccgatcg
      601 atgcctgaag aaatcaatag aattgttacc gatgccttaa gcgattattt atttgttacc
      661 gaaaattcag gattaagaaa tttgaaatat gaaggagcgg ctgacgaaaa gatttttttt
      721 gtaggaaaca ccatgatcga taatttaatt caaaatttag aaaaaattag caataaacac
      781 tttttttcat ctttaaacct caagcttaaa gaatatattg ttactacttt tcatagacca
      841 tcgaatgtgg atagccccga caatttaaat gagatggtga attttttaaa tgaagtagct
      901 aaaaaatatg atatagtttt cccgattcat ccacgaactt tgaataattt gaaaaaattt
      961 caccttttaa agaaaattaa tactaatgtt catttaattg aaccccttgg gtatattaat
     1021 tttttaagtt tagttatcga ctcgaaaggt gtggtaaccg acagtggagg aatacaagag
     1081 gaaacatgtt tcttaaaaat tccgtgtata accgtaagaa attctacaga aagacccata
     1141 actgtcgaaa tgggaacgaa tcatcttgta ggatccaatt tcgatgctgc attaaatgcc
     1201 atagaagtat ttaataattc cttgcaaaat acaatgatac cgcctttatg ggatgggaaa
     1261 acttcaaaaa gaatagtgga aataatttta catcatttgc agcctcaatg aaaattggta
     1321 tgctacttga caatgaattt gttagtgacc caagagtatg gaatgaggct ttaatgttga
     1381 ctcaaaaggg acatgaggtt catgtaatct gcctcaattt tggaaaacaa aataaatatg
     1441 aattaaaaaa taaaattcat atacatagaa ttaccatacc acggaaaatc aagaatttat
     1501 tgtttataac tcaactaatc ttcccaggat ataaaatatt ttggtactat ttttctaaga
     1561 aaattatcca aaaatataat ttaagagtta ttcatgcaca tgatctttat atggctttgc
     1621 cagctatatt gctaaagcaa atgtataata tcaaaataat tcttgattta catgaaaact
     1681 ttcctgcagc tgttaaggct tatacatggg cgcaaaaaaa gcatgtaaaa tggatattta
     1741 aagcaagtag atgggaaaga attgaaaatg gcttactcca gaaatcagat gcagttatag
     1801 ttttgtctaa ttattttaaa aatactttaa taaagaaata taataattta aattctgata
     1861 agatttatgt ttatcctaat gtcccgaata tagctgagtt tcatagcttt aaaatcgatc
     1921 cttatgccta tgagttcatg acaaaaggtt tttggatggt ttattttggt gttgttgcaa
     1981 ttagacgagg gataacttac cttcttgaag ctgtcgacag aataagcaat ccagaaataa
     2041 agcttttgat tattggccca attgataagc atgacagaga gttattcgaa cccaaattca
     2101 gaaaatatga gaaggaaggg aagattattt attatccgtg gaaagatatt tctgaacttc
     2161 cttctttatt atttcatgct aaacttggtg tgagtcccat tcttcgaaat ccccaacatg
     2221 aatctggggt tgcaaataaa gtttttcaat atatgctact tgaaaaagcc atattagttt
     2281 ctgattgtaa gcctcaggtt gatataataa aagaatacca ttgtggttta tattatcagg
     2341 atagtaatat tgatgatttg atagaaaaaa tattatggtg ttatcgaaat actgatgccc
     2401 ttactgaaat gggtcggaga ggaagaaaag ccgttataga aaaatataat tcggatgtac
     2461 agtttcaacc tatcatagaa ttttataata atttagaaag ctttggataa taaaattatt
     2521 tatgaaaatt ataattgggg aagcctgggt acaaacaagc tcggcccaaa aatcaagaaa
     2581 atcattgaaa atatcccttc tgatgtacaa tcaattatag atattggctg tggcaatgga
     2641 gtgattacca acgaactggc aaaaaagtac aaagttctcg gagttgaccg tagcaagtcg
     2701 gcattacaat atgtaacctc tgagaaattg gaggcttcgt gcgacaacat tcccctgcct
     2761 tcacagaact tcgatatggt ctttt
//
LOCUS       DAORPD010000127         2770 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_6508_length_2770_cov_3.366483, whole genome shotgun sequence.
ACCESSION   DAORPD010000127 DAORPD010000000
VERSION     DAORPD010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6508_length_2770_cov_3.366483"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..1831)
                     /locus_tag="PK976_02190"
     CDS             complement(71..1831)
                     /locus_tag="PK976_02190"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRC66729.1"
                     /translation="MKTIRKFTAMSLAVMALLSLTCLTSCKDDEDENLVIAGFTYQVD
                     SSNFLKVSFTNTSQNYTTCNWNFGDGQTSSEVNPVHIYAAEGEYTVTLTAKDANGNQD
                     VYSTKIVLSDPNVLLTALTGMESKTWKLIRDVTTGRYPLECGPEDHSQIWWAMGRDNN
                     ELANRPCQLNDEWIFYRDGRMEFRAQGDYWAEGGLFSPENQCNTTDQMVGPNGEDLSA
                     WGDGNHTFKLTVGNPSKIKAIGKGAYIGFYKLGNGTEVKTPQDYVEYNIVKLVDGTVD
                     TLIVEGIYRWDPASSGGYWRFVLVHYDNPADEPPIPGPKPEASFSMQMDGRTVTFTNT
                     TENGETYLWDFGDGNTSTEKDPVHTYASDGIYMITLTASNPNGESTVTKQAFVTGTEL
                     TAELLMGGAWRPRAENLSIFVGPGLGDYSWWSVPKNFLDGSTSGTDDWSCITDDEFTF
                     ADGGVYIYNTNGSARNDGYMGSPNGCWADEQIAASGNGAAFGSGTHSWEFVPASGGSR
                     PLIILTNGPTGAAFLGFYKGYYGGENSDGANPPNGGNLTNRYEVMGYANTGTKEYLFV
                     SVDISADHSGSSAWSVILER"
     gene            2299..2751
                     /locus_tag="PK976_02195"
     CDS             2299..2751
                     /locus_tag="PK976_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66730.1"
                     /translation="MQLPLPFFPTDTRMINSNVGVFSKDEFVYFLFNGSPIYCCLKDD
                     LNNFRFIVANLVVNHLCTCSEISHALGIHVRNVQRYVKALNEKGVEWFFNREERRGQC
                     FKFTESVKKEAQTMIDKGRTPYSTAKELGLSESTIYYHVRKGNLKKKN"
BASE COUNT          821 a          561 c          556 g          832 t
ORIGIN      
        1 agcttaatta ggaataagaa agaatctggg gtcaattgtt gaccccagac ttgaaccaga
       61 tgcaaaaacc ctatcgctca agaattaccg accaagctga agaaccgctg tggtctgcgc
      121 tgatatcaac ggagacgaag aggtattctt ttgttccagt attggcatat cccatcactt
      181 catatctatt ggtaaggtta ccgccattgg gaggattagc tccatcggaa ttttcaccgc
      241 cataataacc tttgtagaag ccgaggaaag ctgctcctgt ggggccattg gtaaggatga
      301 tcaaaggacg gctaccccca gaggctggaa cgaattccca tgaatgtgtt ccagaaccga
      361 aggcagctcc atttccgctg gctgcaattt gttcgtcagc ccagcatcca ttgggcgaac
      421 ccatataacc gtcattgcga gcactaccat ttgtgttgta aatatatact cctccatcag
      481 caaatgtgaa ctcatcatcg gtgatgcatg accaatcatc agtaccagaa gtgcttccat
      541 cgaggaagtt tttgggaacg ctccaccatg aataatcgcc caaaccaggg ccaacgaata
      601 tcgaaagatt ttcggctctt ggtcgccatg cgccacccat taaaagttcg gcagtcaatt
      661 ctgtacctgt aacaaaagcc tgtttggtta ctgtactctc accattgggg ttgcttgcgg
      721 taagggtaat catataaatt ccatcactgg cataagtatg cacgggatct ttctcggttg
      781 aggtattgcc atccccaaaa tcccataaat aagtttcacc gttttcggta gtattggtga
      841 aagttacagt acgaccatcc atctgcatgc taaaagaggc ttcaggtttt ggccctggaa
      901 taggaggttc atcggcagga ttgtcgtaat gaaccagtac aaatctccaa taaccacccg
      961 aagaagccgg atcccaacga tagatacctt ctacaatcaa tgtatcaaca gtgccatcga
     1021 ctaattttac aatattatat tcaacataat cctggggtgt tttaacttca gtgccattac
     1081 ccaacttgta gaaaccaatg taagctcctt taccaatagc tttaatcttg gatggattgc
     1141 ctacggttag tttgaaagtg tgatttccat ctccccatgc tgataaatct tcaccattag
     1201 gaccaaccat ctgatcggta gtattacact gattttcagg agagaataat ccaccttcag
     1261 cccaataatc gccttgggcg cggaattcca ttcggccgtc acgatagaat atccattcat
     1321 cgttcaactg gcaaggacga ttggcaagct cattattgtc tcttcccatc gcccaccaaa
     1381 tttgagaatg atcttctgga ccacattcca gaggataacg acctgtggtt acatcacgta
     1441 ttaatttcca tgtctttgat tccatgcccg ttaaagcagt caaaagaaca ttggggtcag
     1501 acaggacaat tttggttgaa tacacatcct gattcccatt agcatctttt gccgtaagtg
     1561 taaccgtgta ttctccctct gcagcataga tgtgaactgg atttacttcg gaactggttt
     1621 gcccatcacc aaaattccag ttacatgtgg tgtagttttg cgaagtgtta gtaaaactta
     1681 ccttgagaaa attactggaa tcgacctgat aggtgaagcc agcaatcact aagttttcat
     1741 cctcgtcgtc tttgcaggac gtgaggcagg taagactgag cagtgccatt acggcaaggc
     1801 tcatggccgt aaatttccga atcgttttca tgcatgtttg gttttaaggt ttaacatatt
     1861 ggacttctgg gttaagtgtt aaatttaaga ttaatcattg tatctgtttg agtgtaaaaa
     1921 atggattatt gtaaaattcg ccctataatt aatatctatg ttaaaatatt gaataataaa
     1981 attatatact caaaaaacaa tctctatatt tttattagtg tatctgatac aaagatataa
     2041 aaggttttca ataaacaaat cttttacgaa aaaaaaattg aaaatttatt ttcctttcag
     2101 gttatggtag tgtaatcatt gagttaataa ataatgccaa ggtattatga atcattcaat
     2161 tcctgtattc tgatgatgga acgatgaatg cattgtcctc aaatttcaga actcctgatc
     2221 ctataattgc atatcaatat tttacaaaag taccaccgta tttcaatttg tcgtattttc
     2281 gggcaaaaat tttttgatat gcaacttccg ttaccatttt tcccgaccga tactcgaatg
     2341 attaattcca acgttggagt tttttcgaaa gatgaatttg tgtattttct tttcaatggt
     2401 tctccgatat actgctgtct taaggatgat ttaaacaatt ttcgttttat agttgccaat
     2461 ttagtagtca atcatttgtg cacctgttcg gagattagtc atgctttagg cattcacgta
     2521 aggaatgttc agcgttatgt aaaggcactg aacgagaaag gagtagaatg gttttttaat
     2581 cgagaggagc gtcgtgggca gtgtttcaag tttacggaat cggttaagaa ggaggcacag
     2641 acaatgatag ataagggacg tacgccttat agcacagcga aagaacttgg cttaagtgaa
     2701 tctacgatat attatcatgt caggaaagga aacctaaaaa aaaagaatta gcggtttctg
     2761 accaagggag
//
LOCUS       DAORPD010000128         2770 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_6512_length_2770_cov_2.479190, whole genome shotgun sequence.
ACCESSION   DAORPD010000128 DAORPD010000000
VERSION     DAORPD010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6512_length_2770_cov_2.479190"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="PK976_02200"
     CDS             complement(<1..368)
                     /locus_tag="PK976_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HRC66731.1"
                     /translation="MEYSIGPHLAKINSPEELRMLPEDKLPELCQEIRDYIIEVMSSN
                     PGHLAASLGAVELAVAIHYVFNTPSDKLIWDVGHQAYAHKIITGRRDQFPNNRTYGGI
                     SGFPRMEESEYDAFGTGHSST"
     gene            complement(462..785)
                     /locus_tag="PK976_02205"
     CDS             complement(462..785)
                     /locus_tag="PK976_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly protein"
                     /protein_id="HRC66732.1"
                     /translation="MNDSIGKSQLTEEIIKELKILYDPEIPINIYDLGMIYDVEIDDD
                     NNVRILMTLTAPNCPVAESLPADVKKAVENIKGVKSCHVEVTFDPPWDQSMISDAALL
                     ELGLL"
     gene            complement(787..1224)
                     /locus_tag="PK976_02210"
     CDS             complement(787..1224)
                     /locus_tag="PK976_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005928823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HRC66733.1"
                     /translation="MQSIQEKADQIVKELNQFDDWLDKYQYLIEKGKELYAAGSEIKN
                     DTNLISGCQSKVWLVSEYRDGRLYFKGESDALITRGLVSMVIQVFSGSTPDEILSFEP
                     KFIEEAGLSQHLSPSRNNGLLSIIKQIKLYAFAWKIKNNYQNK"
     gene            complement(1238..2452)
                     /locus_tag="PK976_02215"
     CDS             complement(1238..2452)
                     /locus_tag="PK976_02215"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005656052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HRC66734.1"
                     /translation="MDVNAVRNDFPILQQKIYGKPLIYLDNAATTQKPNAVMQAIVRY
                     YETLNSNVHRGNHLLSIQATDAMERARNRIASFIHAPHPEEIIFTRGTTESINLVAQG
                     FASSLLQPGDEVIISALEHHSNLVPWQQACLRTGAQLNIIPLDESGQIRLGDFRNLLS
                     EKTRIVAIAHVSNVLGTILPVQDIIDLAHRRGIPVLIDGAQAVQHLPIDVSQLDCDFY
                     AFSGHKIYGPTGIGILYGKGSWLEKIPPFQFGGEMIEIVSFEKTTFNEIPFKFEAGTP
                     NVEGIIGLAAALDYLCQIGLESIFKYETELFEYALHKLSRLDDVRIIGQAKDRCSVIS
                     FLLDHIHPYDTGTLLDQMGIAVRSGNHCAQPLIDLLNLPGTVRVSLSFYNTREEIDIL
                     ITALQKVKDIFQ"
     gene            complement(2477..>2770)
                     /locus_tag="PK976_02220"
     CDS             complement(2477..>2770)
                     /locus_tag="PK976_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HRC66735.1"
                     /translation="YADDVKCSHGATIGQLDKEAMFYLRSRGIDQDNARLLLMYAFAA
                     EILNRIDIPALKDRLDDLVKKRLRGELSICDQCVLHCKNPEKPIVFDIDLSKL"
BASE COUNT          783 a          597 c          592 g          798 t
ORIGIN      
        1 gtcgaagaat gccctgtccc aaaggcatcg tattcacttt cttccatccg gggaaaacca
       61 cttataccgc cataagttct gttgttcgga aattgatcgc gacggccggt aatgatttta
      121 tgagcataag cctgatgccc gacgtcccag atcaatttat cggaaggagt attaaaaaca
      181 taatgaatgg ctactgccaa ctcaacggct ccgagacttg ccgccagatg cccaggatta
      241 gacgacatta cttctataat gtaatccctg atttcctggc acagctcggg cagtttatct
      301 tcgggaagca tgcgcaattc ttcgggcgaa tttatcttag ccagatgggg tccaatacta
      361 tattccatct caatattcca acaaaatctt tagattgcaa aattacactt acaaaccttt
      421 taggtttaca aaataacgaa aagaaagaat agacaaagca attaaagaag cccaagctcg
      481 agcagagcag catcacttat catgctttga tcccagggtg gatcaaaggt aacttctaca
      541 tggcaacttt tcacgccctt gatgttttca actgcttttt ttacatccgc aggcaatgat
      601 tcggctacag ggcagttagg agccgtaagc gtcatcagaa tgcgtacgtt attatcgtcg
      661 tcgatctcga catcgtaaat catgccaaga tcatagatgt tgatcggtat ttcgggatca
      721 tacaatattt tcagctcctt gatgatttcc tccgttagct ggcttttccc aatagaatcg
      781 ttcatattat ttattttgat agttattttt tattttccat gcaaaagcat aaagcttgat
      841 ttgctttatt atcgaaagaa gaccgttgtt gcgactaggg gaaaggtgtt gacttaggcc
      901 ggcttcttca ataaatttag gttcgaacga aagaatctcg tcgggagtac tgcccgaaaa
      961 aacctgaatg accatactga cgaggccacg tgtaatcagg gcgtcgcttt cacccttaaa
     1021 atagagtctt ccgtcgcggt attctgaaac tagccacact ttcgactgac atcccgaaat
     1081 cagatttgta tcgtttttaa tttcggaccc tgcagcataa agttccttcc ccttctcgat
     1141 caaatattga tatttatcga gccaatcatc aaactgattg agttctttga ctatctgatc
     1201 ggctttttct tgaatacttt gcatggataa atagttttta ctgaaaaatg tctttcacct
     1261 tttgcagggc tgtaattaaa atgtcgattt cttctcttgt attgtaaaat gataatgaaa
     1321 ctctgacagt tccaggaagg ttcaggaggt cgatcaaagg ctgagcgcaa tgatttcctg
     1381 aacgcacggc tatacccatt tggtcgagca aggtgcctgt gtcgtaagga tgaatatgat
     1441 cgagcagaaa tgagatgacc gaacaccggt cttttgcctg cccaatgatt cgaacgtcat
     1501 ccaatcggct caacttatgg agggcatatt cgaaaagttc agtttcatat ttaaaaatgc
     1561 tttcgagtcc aatctggcag agataatcaa gtgcggcggc aaggccgatg atgccttcca
     1621 cgttaggggt gccggcctcg aacttaaaag gtatctcgtt gaacgtggtt ttttcgaaac
     1681 taacgatttc gatcatttca ccgccaaatt gaaacggagg aatcttttcg agccacgatc
     1741 cttttccgta aaggattcca attcccgtag gaccataaat tttatgcccg gaaaaggcat
     1801 aaaaatcgca atcgagttgg cttacatcta taggaagatg ctgcacagcc tgagcaccat
     1861 ctattaatac tggaatgccg cgtcgatgag ccagatcaat gatatcctgg acaggcagaa
     1921 tcgttcctaa aacattagac acatgagcta tcgccacgat gcgggttttc tcggaaagga
     1981 ggttgcgaaa atccccgagc cgtatttggc cggattcgtc gagaggaatg atatttagtt
     2041 gtgcacccgt acgtaagcaa gcctgttgcc aagggacgag gttcgaatga tgttcgaggg
     2101 cggagataat tacttcgtcg cctggttgaa gcagtgaact ggcaaatcct tgagccacta
     2161 aattgatcga ttcggtagtg ccgcgcgtaa aaataatttc ttcgggatga ggtgcatgga
     2221 taaatgaagc aatcctattt cgggctcttt ccattgcatc cgttgcctga atgcttaaca
     2281 gatgatttcc acggtgcaca ttgctgttta gagtctcata ataacgaaca atggcctgca
     2341 taaccgcatt tggcttttgt gtggtggctg cattatcgag atagattaat ggctttccat
     2401 aaatcttttg ctgaagtata gggaaatcat ttctgacggc atttacatcc agtttcacgg
     2461 caaaatattt taaaacttac aacttactca agtcaatatc gaaaacaatg ggcttttctg
     2521 ggtttttaca gtgaaggaca cattgatcgc agatagataa ttcgcccctc agacgctttt
     2581 tgactaggtc gtcgagccga tccttaagag ctggtatatc gatacgattc aagatttcgg
     2641 cagcaaaagc atacatcaac aaaagtcgtg cgttatcttg atcaatgcct ctggaacgaa
     2701 gataaaacat ggcctcctta tcgagctggc caatagtagc accatgacta cattttacat
     2761 catctgcata
//
LOCUS       DAORPD010000129         2764 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_6540_length_2764_cov_2.395718, whole genome shotgun sequence.
ACCESSION   DAORPD010000129 DAORPD010000000
VERSION     DAORPD010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6540_length_2764_cov_2.395718"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1644
                     /locus_tag="PK976_02225"
     CDS             <1..1644
                     /locus_tag="PK976_02225"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRC66736.1"
                     /translation="AIGSPERHGQRSTLLSYAEIEERGIKYNKDWGTYGGIRKNLAEN
                     FYHKPLLNLNHYWTISNKASLSTSAYFSYGKGGGNWAENFSGKNVFEYQTPSGQVDWD
                     AIARDNLTHQDIYITPQGDTLRHFAKNSQTLFLASHYWAGLISAYSYKPNDQIILTTG
                     IHARYFKSWLWEEIYDLLGAQYFIDDYAWAIESRQGRNILKKRGDTTRIDNGAVVGLA
                     SGFVRANYEQGDLSGLLSFGLNYNTYQREDRYNYPTHPASSIVNKTGYELKAGMAYAF
                     NSQLRVYFNGGYTSRAPYYKFVFPNFNNNPAQNLKNEKITSLEVGMGWQKNDFNLNLA
                     GYYVAWQDKSLLSNEYKLIDNQTTTRSLVTGLDALHEGIEAEAHYSALKSNLNLSAFV
                     SVGNWKWQNNVDAKIFNNENQLIDSVKVYAEGLYVGDAPQFQAGVSALIHFLNQFDFH
                     LSYIWNDRFYADFDPALRTKPEDAQSWRIPGYGLTDVRLGFHFEMMGMETELALACNN
                     LFDVEYITRGNDGIHHNKETFRGYWGPGRTFKLSLEIKF"
     gene            2192..>2764
                     /locus_tag="PK976_02230"
     CDS             2192..>2764
                     /locus_tag="PK976_02230"
                     /inference="COORDINATES: protein motif:HMM:NF023069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA N-terminal domain-containing protein"
                     /protein_id="HRC66737.1"
                     /translation="MDRHETKTNEAREKWAKCMKVIEDNIPPASFKTWFIPIEPLKFE
                     NNVLTIQVPSQFFYEWLEEHYIDLLKKTIRKELGPDCRLEYSIIMDNCYPLSDNITMR
                     LPASNRNTIENPDVSIPIDINPDGLAREIPNPFIIPGLKKIKIQSQLIDTLSFENFVE
                     GDCNQLARSAGYAIAQNPGKTSFNPFFIYSA"
BASE COUNT          904 a          546 c          544 g          770 t
ORIGIN      
        1 gctattggtt cacctgaacg acatggacaa agaagcactt tgttatcgta tgccgaaatc
       61 gaagaaagag gaatcaagta caataaggat tggggaactt atggaggaat acgcaagaat
      121 cttgccgaga acttctatca taagccactt ttaaatttaa accactattg gactatcagt
      181 aataaagcct ctctttcaac ttcggcctat ttcagctatg ggaaaggagg aggcaattgg
      241 gccgaaaact tcagtggaaa aaacgtattc gaatatcaga cgccttcggg ccaagtcgac
      301 tgggatgcca ttgccagaga taatctcacc catcaggata tctatattac accccaaggc
      361 gatacattgc gccattttgc aaaaaactcc caaacccttt tccttgcttc acattactgg
      421 gcaggactta tctcggctta tagttataaa cctaacgatc aaataattct cactaccgga
      481 atccatgcac gttatttcaa aagctggcta tgggaagaaa tttacgactt gctgggagca
      541 caatatttta tcgatgatta tgcttgggct attgaaagtc ggcagggaag aaacattttg
      601 aaaaaaagag gagacacgac gagaattgat aacggggctg ttgttggact ggcttcgggc
      661 tttgtaaggg caaattatga acaaggcgat ctgtcgggat tgctatcatt cggactcaac
      721 tacaatacct atcaaagaga ggatcgctac aattatccca cacatccggc aagttccatc
      781 gtcaataaaa ccggatacga gctgaaagcc ggcatggcgt atgcattcaa ctcacaatta
      841 cgagtttact tcaacggagg ttatacttcg agagcccctt actacaagtt cgtgttcccc
      901 aactttaaca ataatccagc tcaaaacctc aagaacgaaa aaattacttc gctagaagtt
      961 ggaatgggat ggcaaaaaaa cgacttcaac ctcaacctcg cgggatatta tgtggcatgg
     1021 caggacaaat cactcctttc aaacgaatat aaactgatcg ataatcaaac tacaacccgc
     1081 tcgctggtta caggtctgga tgcattgcat gagggtatag aagccgaagc ccattattcg
     1141 gcattgaaat ctaatctgaa tctttcggct tttgtttctg ttgggaactg gaaatggcaa
     1201 aacaatgtgg atgcaaaaat attcaacaac gaaaatcagt tgatcgacag tgtaaaggtt
     1261 tatgcggaag gtttgtatgt gggtgatgcg ccacagtttc aagccggagt ttcggcgctg
     1321 attcatttcc tgaatcaatt cgattttcac ctgtcttata tctggaacga ccgattttac
     1381 gccgatttcg accccgccct aaggacaaaa cccgaagatg ctcagtcatg gcgaattccc
     1441 ggatatggat taaccgatgt tcgcttggga tttcatttcg aaatgatggg aatggaaact
     1501 gagctggcgc tggcttgcaa taaccttttc gatgtggagt acatcacccg tggaaacgat
     1561 ggaattcatc ataataagga aaccttcagg ggctactggg gaccgggaag aacctttaag
     1621 ttaagcctcg aaataaaatt ttaatcctta ttgacacctg acacttaaaa caataggcgt
     1681 caatctcatt gggatataac tatttattta ttgataaaca ggtagttaag gaataactcc
     1741 ctgactgatt ctctcattat actcatttat aatcaatcat ctctatttag aatgattcca
     1801 atattaaatt tttcattttt ggtttgccca tttgaaaaat tcctttaatt tagacggtgg
     1861 gaaaaactcc ctccaaatac tttaataaat tgaaaaacaa atgattatat taataattca
     1921 ttattatgcc tttatgatag ataaaccttt attttaacag aatatttatc tcttgagatg
     1981 aatagaagtg caaaataaaa ctttgaaaac caaaccaaaa cgagtttcaa gaaaagtgta
     2041 tacacaaaga attgaaaaat gcaaggagga aaaggctttt tttcaaaaat tttacacaca
     2101 attttgttga agacttttgg agagggggca caaatgagca aaattgtatt tattgaactg
     2161 aatgataact taaaaatatc aacgaatgta tatggataga cacgaaacta aaaccaatga
     2221 ggcccgagaa aaatgggcaa agtgtatgaa agtcatcgag gataatattc ctccggcaag
     2281 tttcaagact tggtttattc ccatcgagcc gctgaagttt gagaataatg tcttaaccat
     2341 tcaggttccg agtcaatttt tctatgaatg gctcgaggag cactatatag accttttgaa
     2401 aaagacaatc agaaaagaat tgggacctga ttgccgtctg gaatacagca tcatcatgga
     2461 taattgctat ccgttatccg ataacataac catgcgtttg cccgcttcga atcggaatac
     2521 catcgaaaat cccgatgtaa gcattcctat cgacattaat cctgatggct tggccagaga
     2581 aattcccaac ccttttatca taccaggttt aaagaaaatc aagattcaat cacaattaat
     2641 cgatactctg tcatttgaaa attttgtaga aggcgactgc aaccaattag ctcgttcggc
     2701 tggatatgcc attgctcaga atccgggcaa aacctccttt aatccttttt ttatttacag
     2761 tgct
//
LOCUS       DAORPD010000130         2760 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_6550_length_2760_cov_2.501294, whole genome shotgun sequence.
ACCESSION   DAORPD010000130 DAORPD010000000
VERSION     DAORPD010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6550_length_2760_cov_2.501294"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="PK976_02235"
     CDS             <1..579
                     /locus_tag="PK976_02235"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRC66738.1"
                     /translation="EIIEGCIAGKRYFQNLLYEKYKRSLFSVCLRYAVNKQEAEDILQ
                     ESFCKIFSRLGDYKAHGSFEGWLKRIVVNYTLSYLKQNKKDPLHFNNAEVVENIADDE
                     EENDNDLFKNISPQHCQTILKFIQELPVGYRTVFNLYAFEGYSHQQIAQTLQISENTS
                     KSQLSKARRHLKNRIHKWMEKGITTNNPSSSY"
     gene            579..1880
                     /locus_tag="PK976_02240"
     CDS             579..1880
                     /locus_tag="PK976_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66739.1"
                     /translation="MEEKHNHIDTYIRQSLDGFEPEPSERVWKGIKRKLAWNEFVHFN
                     ITNFTSNIYQISATVLIGAGIIVGLVTQLLLVDKIRQQAQVPLAEKDIPTIIQPESPK
                     PEEKLFDSSSPIRSELSEKPLPSSISFLPSSPSFAPEPTIESLETPGLETSIAKKTAF
                     AIPLLPSHGIDRISLALPPSQLYQPLQKPGDSIAFGFEEPEKYPYRFGLEAGWWYNQM
                     TLPYPGKDFSYYWNAVGVAFEVEHRNFYAKTGLNFSRMFDRLPFDISYKSLDSIRYVY
                     NVHYYVPDPSHPDNIILITSRETLYDSITHNQNIFTTGTYNYLNIPFQLGCKFLKTGS
                     FAFDAYAGCGLQFLISKKEPLPVIFNYMKTGLNLNPSLVDHYNFNYFFFGGLNIRYFI
                     NENLKLELQPVYQYYMKSLGDHEGAGNPYSWGLKIGIGYYF"
     gene            1966..>2760
                     /locus_tag="PK976_02245"
     CDS             1966..>2760
                     /locus_tag="PK976_02245"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRC66740.1"
                     /translation="MYNSKVYIRLIKVAAFLLPYMALIVSNAERLQAQEGPRSECQAS
                     FSYTYDSMNFLGIYFIDQSTGSFGSWQWDFGDGWGSSEQNPFHAYTQAGQYHVCLTIS
                     AEGKVCYDVECKDVRVPRPSNCQSTFTWAADSANPFFIRFTCHPGEDADDYFWSFGDG
                     DTSSLCNPCHVFADTGVYIVTLISSNRLNPSFCYSVYIDTVHIKIEPCISSFFAHPSL
                     FNPLEVQFIATPSGNIDHYYWDFGDGRTSMEPNPIYIYSDTGIFTVC"
BASE COUNT          792 a          576 c          518 g          874 t
ORIGIN      
        1 gaaatcatcg aaggctgcat agccgggaaa cggtattttc aaaacctgtt gtacgaaaag
       61 tacaaacgtt ctttgtttag tgtttgcctg agatatgcag taaacaaaca ggaagctgaa
      121 gatatacttc aagaatcatt ttgcaagatt ttcagccgtt taggcgatta taaggcgcat
      181 ggttcgttcg aaggctggtt gaaacgtatc gtcgtcaatt ataccctttc ctatctaaag
      241 cagaataaaa aggatcctct tcacttcaat aatgccgaag tggtcgaaaa tatagctgac
      301 gatgaagaag aaaatgacaa tgatcttttc aagaatatat cgccacaaca ctgtcaaacc
      361 attctgaaat tcattcagga acttcctgta ggctatcgaa cggtgtttaa cctttatgcc
      421 ttcgaaggct actcacatca gcagattgcc cagacattgc agatctcgga aaatacgagc
      481 aaatctcaat tatcgaaagc ccgtcgccat cttaagaaca gaattcataa gtggatggaa
      541 aagggcataa ctaccaataa cccaagttct tcatattgat ggaggagaaa cataaccata
      601 tcgacacata tataaggcaa tccctcgatg gatttgaacc cgaaccttcc gaaagggtct
      661 ggaaaggaat caagaggaaa cttgcatgga atgaatttgt acatttcaac atcacgaatt
      721 tcacctccaa tatataccaa atttcggcga ccgtccttat cggtgcgggt attatcgtgg
      781 gtctggttac tcaattgtta ttagtcgaca agattcgtca acaagcacaa gtaccgcttg
      841 ctgaaaaaga tattcctacc atcatccagc cagaatctcc aaaacccgaa gaaaaactat
      901 tcgattcatc ttctcctatt cgatctgaac tttccgaaaa accgcttcct tcttcgattt
      961 cttttctccc atcctctcct tcttttgctc ccgaaccaac catagaaagc cttgaaaccc
     1021 caggtctcga aacatcaatt gccaaaaaaa cagcattcgc gattccttta cttccctctc
     1081 atggtataga ccgtatttca ctggctttac caccttcaca attgtaccag cctttgcaaa
     1141 agccggggga ttccattgcc tttggattcg aagaacccga aaagtatcct taccgttttg
     1201 ggctggaagc tggatggtgg tataatcaaa tgactctacc ttatccaggg aaagactttt
     1261 cgtattattg gaatgctgta ggcgtggcgt tcgaagtgga acacagaaat ttttatgcaa
     1321 aaaccggttt aaattttagc cgaatgttcg accgcttacc attcgatatt tcctataagt
     1381 cgctcgatag cattcgttat gtctataatg tacattatta tgttcccgat ccttcccatc
     1441 ccgataatat tattctgatt acctccaggg agaccttata tgattcgata actcataatc
     1501 aaaatatttt cacgacggga acatataatt atttgaacat tccctttcaa ctgggttgta
     1561 aatttctaaa aacaggaagt tttgccttcg atgcttatgc gggatgtggc ctgcaattct
     1621 tgatcagtaa aaaagaacct ttgccggtaa tcttcaatta tatgaagaca ggcctgaatt
     1681 tgaatccttc tttagttgat cactacaatt tcaattattt tttctttgga ggattgaata
     1741 ttcgatactt tattaatgaa aacctcaaac tcgaactgca gcctgtatat cagtattata
     1801 tgaaatcatt gggtgaccat gaaggagcag ggaaccccta ttcgtggggt ttaaaaatag
     1861 gcattggtta ttatttttaa aagaatttac attcttgttc attttttact aaatttgttg
     1921 actgataatt gccttccacc ttcgtgttat atgccatttt aatttatgta taattctaag
     1981 gtttacattc gtcttatcaa ggttgcagct tttttgctgc cctacatggc attaatcgtt
     2041 tctaatgcag aaaggttgca ggcacaagaa ggccccagaa gtgaatgtca ggcttccttt
     2101 agttatactt atgattcgat gaatttttta gggatttatt ttattgatca atcaacgggc
     2161 tcatttggtt catggcaatg ggattttgga gacggatggg gaagttctga acagaatcct
     2221 tttcatgctt atactcaggc agggcaatat catgtttgct tgaccatttc ggccgaaggt
     2281 aaagtttgtt atgatgtaga atgtaaggat gttagagttc caagaccatc gaattgccag
     2341 agcacattta cttgggctgc cgattcagca aatccatttt tcatccgttt tacttgccac
     2401 ccaggagaag atgccgatga ttatttttgg tcttttggag atggtgatac ttcttctctt
     2461 tgcaatccat gccacgtatt tgccgacacg ggtgtttata tagttaccct tatatcgagt
     2521 aatcgcctga atccatcctt ttgttatagt gtttacatcg acacggtaca tattaaaata
     2581 gagccttgca tttcgtcttt ctttgctcac ccttctttat tcaatcctct tgaggttcaa
     2641 tttattgcca ctccctccgg gaatattgat cattactact gggattttgg cgatggccgc
     2701 acttcaatgg agcccaaccc catttatata tacagtgata cgggaatctt cacggtttgc
//
LOCUS       DAORPD010000131         2753 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_6565_length_2753_cov_2.358414, whole genome shotgun sequence.
ACCESSION   DAORPD010000131 DAORPD010000000
VERSION     DAORPD010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6565_length_2753_cov_2.358414"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="PK976_02250"
     CDS             complement(<1..761)
                     /locus_tag="PK976_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018668793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRC66741.1"
                     /translation="MDQKGSPLELGTKRIWPLLLQYSIPSVIAMTASSLYNITASIFI
                     GHGVGALALSGLAISFPLMNLAAAFGSLVGVGASTLMSLRLGQRDYASANKILGNVFV
                     LNILLGLVYTFIVLLFLDPILYFFGASKATISYAHDYMLVITIGNVVTHLYFGLNAML
                     RATGNPTKSMMATISTVIINIILTPLFIFGLKWGIKGAAISTVVSQTLLMIWQIRFFS
                     NKENFIHLQKNSFRLERKIVVDSLSIGMAPFLMNAA"
     gene            complement(1111..>2753)
                     /locus_tag="PK976_02255"
     CDS             complement(1111..>2753)
                     /locus_tag="PK976_02255"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HRC66742.1"
                     /translation="NKRKSEQLVWQYMNHFLENEPVPGPEFEYIFAKNLLPSITLEEV
                     NALPSKWITDSNRVIVVQGPQKEGLIYPTEQEVLDLLARIEKEEITPYVDKVSDKPLI
                     SELPKPGKVIESSEDKATGVITWKLSNGAKVVIKPTDFKEDEILFAAISLGGSSLYPI
                     EEYLSAQFAAAIIGESGLGDFDATELQKALSGKLVDLSPYIDDLKEGFNGRSSKSDLE
                     TLFQLLHLYFGKARADENIYGSHMNRLKAFLENSALDPENTFRDTIQVTMASYSPYRK
                     PLTVERLSETNLSLMKQIFDQRFADADGFTYYFVGSFRPDELKPLIEKYLASLPSVKK
                     NEQWRDLKIQPPKGTIKKTVVRDMEDPKATVFISLTGKFDYDSMKRLAMRAINDILSF
                     RFVETIREEEGATYGTSVRTRLSKYPQPQYQLSIQFDCDPENAERMTHIVYQEIEKLR
                     TQGPTEEQMRNFKENQLKTWAENIKENNFWMNKLTTSDFDGESYENILKFPEMLKILT
                     PEMVKKVAQQCYSGENVVQIILMPSDLSKSVKNPNIKP"
BASE COUNT          800 a          606 c          516 g          831 t
ORIGIN      
        1 gcggcattca taagaaaggg agccatgcca atgcttagag aatctacaac gattttgcgt
       61 tccaatctga aactgttttt ttgcagatgg ataaaatttt ctttattgct aaaaaatcga
      121 atttgccata tcatcaaaag agtttgcgag acaacggtgg aaatggcagc tcctttgatt
      181 ccccatttaa gaccaaaaat aaataaaggc gtcagaatga tgttgattat caccgtagaa
      241 atagttgcca tcatcgattt cgtaggattt ccagtagccc ttagcatggc gttcaatcca
      301 aaataaagat gagtaactac attaccgatg gtaataacca gcatataatc atgtgcataa
      361 gagatagtgg ctttgctggc tccaaaaaaa tagagtatgg gatcgagaaa taagagaacg
      421 atgaaggtgt aaaccaaacc taataggata ttcagaacga aaacattccc caggatttta
      481 ttggccgacg cataatctct ttgtccaagg cgcaatgaca tcaaggtaga agcaccaaca
      541 cctacaagtg atccaaatgc agcggccaaa ttcatcaatg gaaaactgat ggctaaaccc
      601 gaaagagcca aagcaccaac cccatgtcct ataaaaatgc tggccgtgat gttgtaaagc
      661 gaagaagccg tcatggctat caccgatgga atggaatact gcagaagcaa aggccatatc
      721 ctcttcgtgc ctaattccag tggagaacct ttttgatcca ttagtttaat atcaaaaatg
      781 ccctaaaatg accaaataca tttatctgac ctcttccctt atcgtctctt tgaaggaaat
      841 cagaaattcg gcaaaattac cactctttta agattaacct tccaatgtac tacacttccc
      901 cccctccgcc gcactacgaa ggatataatt ctctgtaaag tcgtcagacc tgcaaagcat
      961 ctatgtcttc cactctctct atctctacgt ctccttgcct cttcgcaata cgaaacgttt
     1021 ttttatctgt tgtttcccaa accctattcc atatttcttt ataatccatt ctctattctt
     1081 ccttctccac ccttcaaaac ccttaccttt ttaaggtttt atattcggat tcttcaccga
     1141 tttactcaag tcggaaggca tgagaataat ttgcactaca ttttcgcccg aatagcactg
     1201 ctgagcaact ttcttcacca tttccggagt taagatcttc aacatttcag ggaatttcag
     1261 gatattttca tacgattctc catcaaaatc acttgtagta agtttattca tccagaaatt
     1321 attttccttg atattttccg cccatgtctt tagctgattt tccttaaaat tacgcatttg
     1381 ttcctccgtt ggaccctgag tacgcaattt ctcgatttcc tgatagacga tgtgggtcat
     1441 gcgttcggcg ttctcgggat cacagtcgaa ttgaatactg agctggtatt gtggctgggg
     1501 atatttcgac aatcgagttc taacacttgt gccataggta gctccttctt cttcgcgtat
     1561 agtctctaca aatctaaaac tcaatatatc atttatagct ctcatggcca ggcgtttcat
     1621 ggaatcataa tcgaactttc cggtaagcga aataaatacc gtagccttag ggtcttccat
     1681 atcacgcacc actgtttttt tgattgtgcc tttgggaggt tgaattttca ggtccctcca
     1741 ctgctcgttt ttcttcacgg aaggtaaaga agccaggtat ttctcaatca gaggtttcaa
     1801 ttcatcgggc cgaaagctcc ctacgaagta ataggtaaat ccatcggcat cggcaaaacg
     1861 ctggtcgaag atttgtttca tcaggctgag gttggtttcc gatagtcgtt cgacggtaag
     1921 gggtttacga taaggactat atgaagccat agttacctgt atagtatctc tgaaggtatt
     1981 ttcgggatcg agagcactgt tttcgagaaa tgctttcaaa cgattcatgt gtgaaccata
     2041 aatattctca tcagcacgtg ctttcccaaa ataaagatgc aacaactgga ataaggtttc
     2101 aaggtcgctt ttcgaagaac gaccgttaaa tccttccttc aaatcatcga tataaggtga
     2161 taggtcaact aatttcccac ttagcgcttt ctggagttcg gtagcgtcaa aatcgccgag
     2221 accactttca ccaatgatag cagcagcaaa ctgagccgaa agatattctt cgataggata
     2281 aagagatgag cctcccaaac tgattgcggc aaataaaatt tcgtcttcct taaaatcggt
     2341 aggttttata acgactttag ctccattgga caatttccat gttatcacac ctgtagcctt
     2401 atcttcggaa gattcaatca ctttaccagg tttaggcaat tccgaaataa gaggcttatc
     2461 ggaaacttta tctacataag gagttatttc ttctttctcg atccttgcca gtaaatcgag
     2521 aacttcctgt tcggtaggat aaatgaggcc ctctttctga ggtccctgaa ccacaatgac
     2581 ccgattgctg tccgtgatcc atttcgaagg taaagcatta acctcttcga gggtaatact
     2641 tggcaataag tttttggcaa aaatatattc gaattcaggg ccaggtacgg gttcattttc
     2701 aaggaaatga ttcatatatt gccaaaccag ttgttcagat ttgcgtttat tgc
//
LOCUS       DAORPD010000132         2737 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_6621_length_2737_cov_2.370619, whole genome shotgun sequence.
ACCESSION   DAORPD010000132 DAORPD010000000
VERSION     DAORPD010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6621_length_2737_cov_2.370619"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="PK976_02260"
     CDS             <1..737
                     /locus_tag="PK976_02260"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009433123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HRC66743.1"
                     /translation="ETASGMLNAVGLQNKGIDHFIHEIWPRLKQFKTPFIVNVSGSTI
                     EEYIETAEKLDSLEGMPAIELNISCPNVKEGGMTFGTSCLAAASVTKAVRKVFHKTLI
                     VKLSPNVTDITEIAKAVEGEGADAVSLINTLLGMAIDAQSRKPILSTITGGLSGPAIK
                     PIALRMVWQVYHTVKIPVIGIGGIMNAMDAIEFLLAGAVAVQVGTANFIDPAVTLKII
                     EGINDYCEQNNLSSIKDLIGALIIPE"
     gene            complement(885..1754)
                     /gene="sucD"
                     /locus_tag="PK976_02265"
     CDS             complement(885..1754)
                     /gene="sucD"
                     /locus_tag="PK976_02265"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HRC66744.1"
                     /translation="MSVLVDKNSRVIVQGFTGNEATFHALQMIAYGTNIVGGVTPGKG
                     GTYHLNRPVFDTVKEAVNGTSANVSIIFVPPAFATDATMEAAEGGINLIVCITEGIPV
                     QDMIKVKEYIKAFGCRLIGPNCPGVITVGEAKVGIMPGFIHKRGVVGVVSRSGTLAYE
                     AVNQLTKVGLGQTTGIGIGGDPIPGTTIVEAVQLLMDDPETKGIVVIGEIGGTMEAEA
                     AYWIKDHGTKPVVAFIAGSTAPKGRTMGHAGAIIGGKDDTASAKKTILRQCGIFVVDS
                     PAEIGSRMLEILV"
BASE COUNT          836 a          617 c          508 g          776 t
ORIGIN      
        1 ccgaaacagc ctcgggaatg ctcaatgcag taggtttgca aaataagggt atcgatcatt
       61 tcatccatga aatatggcct cgcttgaaac agtttaaaac gcctttcatc gtaaatgttt
      121 cggggagcac catagaagaa tacatcgaaa cagccgaaaa actggattct ctcgaaggaa
      181 tgccggccat cgagctcaat atctcctgcc ccaatgtcaa agaaggaggc atgacctttg
      241 gaacttcgtg cctggccgct gcgtctgtaa ccaaagccgt aaggaaagtt tttcacaaga
      301 ctcttatcgt aaaattgtct cccaacgtaa ccgatatcac cgaaattgca aaggcggttg
      361 aaggtgaagg tgcagatgcg gtttcactca tcaacacttt gctcggaatg gccatagatg
      421 cacaaagccg caaacccatt ctttcgacga taaccggtgg attgagcgga cctgcgatta
      481 aacccattgc tctgcgaatg gtatggcaag tttatcatac tgtaaaaatt cctgtcattg
      541 gtattggggg cattatgaat gccatggatg ccatagaatt tcttcttgca ggagccgttg
      601 ccgttcaagt tggaacagcc aattttatcg atcctgccgt aacactgaag attattgaag
      661 ggataaatga ttactgcgaa caaaacaatt tatcgagtat aaaagatttg attggagcct
      721 taattattcc cgaataaaca acctttaaaa caaattggtc aaaacgcttc cctctcgatt
      781 tttcttcgtg agcaggattg gaatgtaccc aaggcattat ttcctgataa atgagtagga
      841 aggcgaaaga acctgttaca tttcgatttt tgattccaaa tttcttaaac taaaatctcc
      901 aacattctgc ttcctatctc tgcgggggaa tcgaccacaa aaataccaca ctggcggagg
      961 attgtttttt ttgcactggc cgtatcgtct ttccctccga tgattgcgcc agcatggccc
     1021 atggttcggc ctttgggtgc ggtggaaccg gcaatgaagg ccactacagg cttggtacca
     1081 tgatctttta tccagtaagc agcttcggcc tccatggttc ccccaatttc tcctatcact
     1141 acaattcctt tcgtctcggg atcatccatg agcaattgaa cggcttctac aatggtcgtt
     1201 cctggaatag gatcgccgcc tatgccaata cccgtcgtct gccctaatcc cactttggtt
     1261 aattgattca ctgcttcata agctaaagta ccactacggg aaacaacgcc taccacacct
     1321 cttttgtgga taaaacctgg catgattcct actttggctt cacccacagt gatgactcct
     1381 ggacaattcg gcccaatgag cctgcaacca aaagccttga tatattcttt aaccttgatc
     1441 atgtcttgta caggaattcc ttcagtaata catacaatca ggtttattcc accttcagca
     1501 gcctccatgg tggcatcggt ggcaaaagct ggtggaacaa aaattataga aacattcgcc
     1561 gatgtgccat tcacagcttc cttcacggta tcgaaaacag gacgattcaa atgataagtc
     1621 cctcctttgc ccggagttac tccccctacg atattcgtcc catatgctat catctgcaaa
     1681 gcatgaaaag tggcttcatt gcccgtaaat ccttgtacaa taacccttga attcttgtca
     1741 actaatacac tcattgtaaa ttaagaatgt cgatatctga aacaaaactt gactttctcc
     1801 ctcttcaaat ccaattacga aaagagactt cgaaaaaaga gctataaata tgaacaaaca
     1861 actcgaaaaa agtttggcca aattataaaa attcacacaa agataaaaca acccaggttt
     1921 aaaatgataa agcctcaacg ataaaacaag aaatcgctga attcatcatg aaaatttcgt
     1981 tcggctctag ccctgtgcat cagccttaac tatatttaca ggctttgtat atcgaacaaa
     2041 tatcactcta ctattctcca gttcaaatct attttagata cttaaaacat attgattccc
     2101 atgctactcg aaaaccagct ccgtccacat cgtaggaatt tacaagcctt tatctgaaat
     2161 ctataaaaaa tgcttactgg catgattttc gaaatcaaga acagctcgat ctaaatttcc
     2221 tctcctgctt gatatcagta ttagtaaatg tatgtggtac agcatgatta cacaaaccag
     2281 gcaagatata aacccttatt cgcttcataa tacattaggt tttcaaaatc tatatcaggg
     2341 tataccctgc gtaaaaagat gatccattca gccaaacctt caaaacgtca gaatgtaatc
     2401 cgttttctct gttattttat agctttgccg atatatacga acgaattcac actttcttgt
     2461 gaacatgcaa ggccttgtcg atattatcca tttctgaggt gtcagctgct aaaatacatc
     2521 gattcaggcg atagtctaag ccgaactata aagaggatca agtttgaaat tcgtcgataa
     2581 gattaatagc aaaaacattt tactatcttt accaaatcat taattttaaa aaccagcctg
     2641 agagtttatg cttgtaaaga cttatgggag tgcagtaatt ggggtaaatg cgctgacgat
     2701 aactgttgag gtagatatcg aagcgggggt gaatttt
//
LOCUS       DAORPD010000133         2733 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_6628_length_2733_cov_2.061613, whole genome shotgun sequence.
ACCESSION   DAORPD010000133 DAORPD010000000
VERSION     DAORPD010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6628_length_2733_cov_2.061613"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            194..835
                     /locus_tag="PK976_02270"
     CDS             194..835
                     /locus_tag="PK976_02270"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HRC66745.1"
                     /translation="MKNFTLDIDELQGRLSYHLPGWEAQKNMAPTGRVHIPPADMVPI
                     ESAVLLLLVKNSADYEIVFIQRTEDDGPHSGQISFPGGRREPHDKTLLDTALREATEE
                     IALKCNHLKVLGQLTPLYVPTSNFMIYPFIGFADGCDHFRPQPDEVQKVIRVRLNDIL
                     DRNHRRIEVFTSSAGDSYKAPAYFIEEVRIWGATAMIVSEFCEIISSLDRENR"
     gene            1265..1951
                     /gene="mqnB"
                     /locus_tag="PK976_02275"
     CDS             1265..1951
                     /gene="mqnB"
                     /locus_tag="PK976_02275"
                     /EC_number="3.2.2.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR03664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="futalosine hydrolase"
                     /protein_id="HRC66746.1"
                     /translation="MRILLVTATWLEAKPLMDGFTFLRKYSGNNYSFRYGEHRIDVLV
                     TGMGMVATAFGVGKALRNTQYNLVLDAGICSSFRKEFSPGTVLNVSEEFFPEFGMSDD
                     AGFKYLQDMGFDTSLPTACKNTVLQNPHYNQVMGFKNLATARGATVNLVNLTGRRLNT
                     YHSNIEADIETQEGGAFFFSCLASEVPFIELRAVSNYIGPPDEEGWDIEKAIQNLTYS
                     VHEALNVLPR"
     gene            2000..>2733
                     /locus_tag="PK976_02280"
     CDS             2000..>2733
                     /locus_tag="PK976_02280"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRC66747.1"
                     /translation="MQKKIIVTGAAGFIGSCLVGKLNELGYENLILVDDFSSILKEPN
                     WVNKKYEMCIPREDFLQGVHECSCDIEVVFHLGARTDTTEMNEKVFDALNLTYSKQIF
                     NFCSQYRIPLLYASSAATYGAGELGYSDDHHLIPLLRPLNPYGISKNEFDKWVLQQNI
                     TPPFWAGFKFFNVYGPNEYHKHRMASVVFHSANQIRNTGKVRLFRSHRPDYADGMQKR
                     DFVYVFDVIETLIRFWQHRPVSGIYNL"
BASE COUNT          788 a          466 c          594 g          885 t
ORIGIN      
        1 gatgaagaag aataggacag tattttaagg tcgagaattc ttttgttata aagataccat
       61 cctgttctat tgtttattga ggatattgtt cgtaatttta caaatatgta ttatttgcaa
      121 caaaagtgta ttttgtgttg ttaaaaatag gaattaatta ctctaaggtt ttctggaata
      181 aagaaagtaa atcgtgaaaa attttacact cgatattgat gagttgcagg gaaggctttc
      241 gtatcattta cctggctggg aagctcagaa aaatatggca ccaaccggga gagttcatat
      301 tcctcctgca gatatggtgc ccatcgagag tgcggtttta ttgcttttgg ttaaaaactc
      361 agccgattat gaaattgttt tcattcaacg aactgaagat gatgggccgc attcgggtca
      421 gatttcgttt cctggcggac gtcgcgaacc tcatgataaa acattgctgg atactgcttt
      481 gcgcgaagct accgaagaaa ttgctttaaa atgcaatcat ttgaaggttt tgggtcagct
      541 taccccctta tacgttccca cgagcaattt tatgatttat ccatttatag gatttgccga
      601 tggctgcgat cattttcgac ctcaacccga cgaggtacag aaagtgatca gggttcgact
      661 caatgatatt ttggatagaa atcaccggcg cattgaagtt tttacttcat cggcagggga
      721 tagctataaa gctccagctt attttataga agaggtgagg atttggggtg ctactgcaat
      781 gattgtgagt gagttttgcg aaataatctc ctcactcgat cgagaaaata gataaatttt
      841 tgccttaaat cgattattct gcctcaaatg atgattcaat aaatcttgaa gtagctaaac
      901 ttttcttcca gaggagcgtt gttatggtaa gatattttat gacaaatagt tgaattttgt
      961 agtgaatttg cttttttaaa tgcccccaaa tgtcatagat tgaggatatt tcttttgtaa
     1021 ctgaagcagt ggattgtttg ccaggaagaa tttatgcacc ccgtttttta caacattatt
     1081 ttgcgtagat cgaagaaata tcgtaatttt gcatgcgaac gaagaatttt agaatcatct
     1141 caagaatttt aaagagaacg agccaagaat acccttacga taattttatt tttgtagttt
     1201 tgcccccgaa atggggtttg taatcagtgg agatatattc gagtaatttt ataaagcctt
     1261 tacaatgcgt atcttattag ttacagctac ttggctggaa gcaaagccct tgatggacgg
     1321 gtttaccttt ttacgcaagt attctggaaa caattattct tttcgatacg gtgagcatcg
     1381 catcgatgta ttagtaacgg gtatgggcat ggtagccact gcttttggag taggaaaagc
     1441 tctgcgaaat actcaataca atttggtgct cgatgcagga atatgcagtt cttttcgaaa
     1501 ggaattttct ccgggtacgg tgctcaacgt aagtgaggaa tttttcccag aatttgggat
     1561 gagtgatgat gctggattta agtatttaca ggacatgggg ttcgatacca gtttgcctac
     1621 cgcatgcaag aatactgtgt tgcagaatcc tcattataat caggtaatgg ggttcaagaa
     1681 tctggcaacg gctcgcggag ctacggttaa tttggtaaat cttacgggaa ggcgtctcaa
     1741 tacttatcat tccaatatag aggccgatat cgaaactcaa gaaggaggag catttttctt
     1801 tagctgcctt gccagtgaag tgccttttat cgaattaaga gcagtttcga attatattgg
     1861 ccctcccgac gaagaaggat gggacatcga aaaagctatt caaaatctca cctattccgt
     1921 tcacgaagcc ctaaatgtgc ttccgcgcta aagtgtataa ttttttgatc tgatagattg
     1981 attcattttt ttcatcgaaa tgcaaaaaaa aattatcgtt acgggagctg caggctttat
     2041 aggaagctgc cttgtgggta aattgaatga attgggttac gaaaatctga ttttggtaga
     2101 tgatttttcc tcaattttaa aagaacccaa ttgggtaaat aaaaaatatg agatgtgcat
     2161 acccagagaa gattttttgc aaggggttca tgagtgttcg tgcgatatcg aggttgtttt
     2221 tcatttgggc gcccgtaccg atactaccga aatgaacgaa aaagtattcg atgccctcaa
     2281 tctcacttat tccaaacaga tttttaattt ttgcagccaa tatcggattc ctttattata
     2341 tgcctcgagc gcagctactt atggtgctgg agagttgggt tacagcgacg atcatcatct
     2401 tattcctctt cttcggccat taaatcctta tggcatctcg aagaacgaat tcgataaatg
     2461 ggttcttcag cagaacatca ctcctccttt ttgggctggt tttaaatttt ttaatgttta
     2521 tgggcctaac gaatatcata aacatcggat ggcatcagta gtttttcatt cggcaaatca
     2581 aattaggaat acaggtaaag taaggctgtt tcgttcgcac aggcctgatt atgccgatgg
     2641 catgcagaaa agagactttg tttatgtttt cgatgtgata gaaaccctga ttcggttctg
     2701 gcaacataga cccgtttcgg gaatttataa tct
//
LOCUS       DAORPD010000134         2732 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_6634_length_2732_cov_2.442286, whole genome shotgun sequence.
ACCESSION   DAORPD010000134 DAORPD010000000
VERSION     DAORPD010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6634_length_2732_cov_2.442286"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            276..389
                     /locus_tag="PK976_02285"
     CDS             276..389
                     /locus_tag="PK976_02285"
                     /inference="COORDINATES: protein motif:HMM:NF012310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66748.1"
                     /translation="MTMEIHYTKHHRAYYDNLMNAMVFPSWASMFGNMHII"
     gene            341..493
                     /locus_tag="PK976_02290"
                     /pseudo
     CDS             341..493
                     /locus_tag="PK976_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009162597.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
     gene            841..2289
                     /gene="sufB"
                     /locus_tag="PK976_02295"
     CDS             841..2289
                     /gene="sufB"
                     /locus_tag="PK976_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013815481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HRC66749.1"
                     /translation="MPNDKDDILNEITNSDYKYGFVTNIETEFAPKGLSEDIIRYISR
                     KKDEPDFLLDFRLKAYQRWKEMQHPQWAHLDIPPIDYQDIIYYAAPKVNKGPSSLDKV
                     DPELLSTFEKLGIPLEEQKRLTGVAVDAVMDSISVKTTFQETLNKLGIIFCSMSEAIR
                     HYPDLVKLYLGSVVPYADNFYAALNSAVFSDGSFVYIPKGVRCPVELSTYFRINAAKT
                     GQFERTLIVADEGAYVSYMEGCTAPMRDENQLHAAVVEIVALKDAEVKYSTVQNWYPG
                     DREGKGGIYNFVTKRGLCKGSYSKISWTQVETGSAITWKYPSCILQGYNSRGEFYSVT
                     VTNHYQQADTGTKMIHLGRNTSSIIISKGISAGFSNNSYRGLVRVAKKAENSRNYSQC
                     DSLLLGDKCGAHTFPYIQVENKSSIVEHEATTSKISDDQLFYCKQRGIDTESAIGLIV
                     NGYAKEVLKQLPMEFAAEAQKLLAISLEGSVG"
     gene            2340..>2732
                     /locus_tag="PK976_02300"
     CDS             2340..>2732
                     /locus_tag="PK976_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRC66750.1"
                     /translation="MLLQIKNLHVAIQGKEIIKGIDLVVNEGEIHAIMGPNGTGKSTL
                     ASVIAGREDYEVTEGEIVYKGKNLLEMPVEQRAREGIFLSFQYPVEIPGVSMANFMRT
                     ALNDIRKYKGLEPLSGSEFLLRMEEKKQL"
BASE COUNT          841 a          486 c          592 g          813 t
ORIGIN      
        1 agacggcttg actctggcga taagaaaata attttctgtt ccttatgata gatctagata
       61 ttttctccgc tccatcattg attgaaacat cattatccag aattttaccg gtcatctttc
      121 gattttatcg ttgctatgtg aacctttctt gtttttcaat gtttatttag aaatattctt
      181 aataagctaa taaaatcatg aatacactga caaaagattt aatttttccg aaattaggga
      241 tggggtataa tgaccttgaa ccttatatcg acgcgatgac catggaaatt cattatacca
      301 agcatcatcg tgcctattat gataatttga tgaatgccat ggttttccca tcctgggcct
      361 cgatgtttgg gaacatgcat attatttaaa ataccagaac cgccgtccag attatattgc
      421 cgccttcttg aaaatagtta attggaaaat gatcgaagaa aattaccgaa atgctctttc
      481 catgatcaga taattgattt ttgaatgttt tttgatttta gtgattgatt ctcgatatga
      541 aagtggttgg gagtcagtac attttttgat cgagattgac atgatagata aaagcaccga
      601 aagggtttaa aattttataa aaagacagaa tcaataatag atgatgtgcc ttgaataatt
      661 acttagcttg tttttatttg aacctcattt ttaaagccag aaatgccgaa agttgtctaa
      721 ttttgcagat cattccttgg aaactcgacg tgatttttta aaaataaatg tatcgtcaat
      781 tcatgatagt tcttgcgaat tcgacagaaa tacaaacgcg agaggaatta aattttagtt
      841 atgcctaacg acaaggacga tattttgaat gaaataacaa attccgatta taaatacggg
      901 ttcgttacca atatagaaac cgagtttgct cccaaagggc tttcggaaga tattattcgt
      961 tatatttctc gaaaaaagga tgagcccgat tttttactcg atttcaggtt gaaggcttac
     1021 cagcgatgga aggaaatgca gcatccacaa tgggctcatt tagatatacc acccattgat
     1081 taccaggata ttatatatta tgctgctcct aaagtcaata aaggcccgtc ttctttggat
     1141 aaagttgatc ccgaattgtt aagtactttc gaaaaacttg gtattcctct cgaggaacag
     1201 aagcgcctga ctggcgtggc tgttgatgcc gtaatggata gcatctcggt aaaaacgaca
     1261 tttcaggaaa ccctcaacaa actcggcatc atcttttgtt cgatgagtga agcaataaga
     1321 cattatcccg atctggtaaa gttatatctg ggtagtgttg taccctatgc cgataatttt
     1381 tatgcggctt tgaattcggc agttttttcc gacggttcat tcgtttatat tcctaaaggc
     1441 gtgcgttgtc ctgtcgaact ttctacttac ttcagaatca atgcagccaa aacgggtcag
     1501 ttcgagcgca cgctgattgt ggccgatgaa ggagcctatg taagctatat ggaaggatgt
     1561 acggctccta tgcgcgatga gaatcagctt catgctgccg tagtcgaaat tgtagcatta
     1621 aaagatgccg aagtgaagta ttcgacggtt cagaactggt atcctggcga cagggaagga
     1681 aaggggggca tatataactt tgttaccaaa cgtggtttat gcaagggaag ttattcgaag
     1741 atatcatgga cacaagtcga aaccgggtcg gccattacct ggaaatatcc aagctgcatc
     1801 cttcaggggt ataattcaag aggcgagttt tattccgtaa ccgttactaa ccattatcag
     1861 caagccgaca ctgggactaa gatgattcat cttggccgca atacgtctag cattatcatc
     1921 agcaagggaa tatcggcagg ttttagcaat aacagctatc gaggattggt aagggttgca
     1981 aagaaagccg aaaattcccg aaattacagt caatgcgatt cattgttgtt gggtgataaa
     2041 tgcggtgcac atacttttcc ttatattcag gtcgaaaata aatcatccat cgttgagcac
     2101 gaggctacta catcgaaaat ttcagacgat cagctgtttt attgcaagca aagaggcata
     2161 gatacggaat cggccattgg attgattgta aatggatatg ccaaggaagt attgaaacaa
     2221 ttacccatgg aatttgccgc cgaagctcag aaattgctcg ctatcagtct cgaaggaagc
     2281 gttggttagc gtgtcttatc gaataatttt atcaatcgaa aaaatcaaac agaaatatca
     2341 tgctattaca gattaaaaat cttcatgttg ccattcaggg gaaagaaatt ataaagggaa
     2401 tagatttagt ggtgaacgaa ggtgagattc atgccatcat ggggcctaat ggaacgggga
     2461 agagcacttt ggcttcggtg attgcaggcc gtgaagatta tgaagttacc gaaggagaaa
     2521 tcgtgtacaa gggcaagaat cttctcgaga tgcctgtcga acagcgggca cgtgaaggca
     2581 ttttcctgag ttttcaatat cccgttgaga tacctggcgt aagtatggct aattttatgc
     2641 gcacagcctt gaatgacatt cgcaaataca aaggcctcga acccttgagt ggttcggaat
     2701 ttctgttgag gatggaagaa aagaaacaac tg
//
LOCUS       DAORPD010000135         2727 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_6658_length_2727_cov_2.058383, whole genome shotgun sequence.
ACCESSION   DAORPD010000135 DAORPD010000000
VERSION     DAORPD010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6658_length_2727_cov_2.058383"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="PK976_02305"
     CDS             complement(<1..732)
                     /locus_tag="PK976_02305"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRC66751.1"
                     /translation="MRNLTYKAFMVFLLSLFSFSEVYAQKVAVVLSGGGAKGACHVGL
                     LKALEENDIPIDYIAGTSMGAIVAGLYAAGYSPEEMEVLMTSEEFKKWAKGVIDTEYM
                     YYYKKDPNDPSWVSVPVEISPKKFKPKLPTNLVSPYEMDFQFIRIFADAGAAANYNFD
                     SLFVPFRCVAADITANQPYIARSGNLENAIRASMTFPLYFKPITIDGHLMFDGGIFNN
                     FPVDVVLEDFKPDYIIGSKAAGDYSI"
     gene            complement(909..2090)
                     /locus_tag="PK976_02310"
                     /pseudo
     CDS             complement(909..2090)
                     /locus_tag="PK976_02310"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(2478..>2727)
                     /locus_tag="PK976_02315"
     CDS             complement(2478..>2727)
                     /locus_tag="PK976_02315"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HRC66752.1"
                     /translation="IEGNYSEIITKLDINTNDFVVVVTHEHALDISLTAAIATLRPAY
                     IGMTGSKSKSREARKTLQEKYYLDKELIIKIDLPIVYP"
BASE COUNT          770 a          589 c          546 g          822 t
ORIGIN      
        1 gatggaataa tcgccggcag cctttgaacc tatgatgtaa tcgggtttga aatcctccag
       61 taccacatct accggaaaat tattgaaaat tcctccatca aacataagat ggccatcaat
      121 agtaatcggt ttgaaatata atggaaaggt catggaagcc cgaatggcat tttcgagatt
      181 tccactgcgc gcaatatagg gctgattagc agtaatatcg gcagctacac aacgaaaggg
      241 aacaaagaga ctgtcaaaat tgtaatttgc tgctgccccg gcatcggcaa aaatccttat
      301 aaactgaaaa tccatttcgt aaggtgaaac cagattggtg ggtagtttgg gtttaaattt
      361 cttaggacta atttccacag gcactgaaac ccatgaagga tcattgggat ctttcttgta
      421 gtaatacata tattcagtat cgataactcc ttttgcccac ttcttgaatt cttcggatgt
      481 cataaggact tccatttctt cgggagaata gcccgctgca tataacccgg ctactatggc
      541 tcccatgctg gttccggcaa tataatcaat gggaatgtca ttttcttcca aagccttgag
      601 caatcctaca tgacatgctc cttttgctcc acctccactt aataccacgg ccactttctg
      661 ggcataaacc tcagaaaaac taaataggga taaaagaaaa accataaagg ccttatatgt
      721 caaatttcgc atcgaaaaaa attcttttcc aaaattaggc tatatagttg atatgatgat
      781 gcagagcagt tacacaaaac tatatgatgc ttttcaaaaa acacgaattg aatgtccgct
      841 cgaaattcat gaaaaattct catcttcaag agccatcgag ggatgaagtt tcgaagaaga
      901 tggggaattc aaattaaatc gagagcctga gagagatcgg ccacaatatc ttctgcttct
      961 tctattccaa ccgaaagacg aatcaggcca tcactgattc cggcttgatg tcgagcctcg
     1021 gggctcattt ttgaatgagt catgctggcg gggtgctgaa taagcgtttc tattccgccc
     1081 aaactcactg ccaataaggc aagttttaca ttatccatca cgattttacc ggcgggtagg
     1141 cctcctttta cctcgaaact aatcattgct ccaggacctc tcatttgttt acaagccaat
     1201 taatattgtg gatgagattt caaacctgga tatttcaccc aagctacttt ggggtgattt
     1261 tccagaaatt cggccacttt catggcattg gcctgagaac gttccatgcg taaggctaaa
     1321 gttttcaaac ctcttctgat cagccaggct tgatgaggat ccatgttggg ccccatgttg
     1381 gtcatcacaa tgcgtaaagc tttgtaatcg gcttcggttt tggtaattat cattcctcct
     1441 acggcatcgg catgtccatt gataaatttg gtcagactat gcaaaacgac atcggcacct
     1501 aattcgagag gattttgcag ataagggctg caaaaagtgt tatccacgca aactttgatg
     1561 tttctggcat gagccatctg gcttattgcg gcaatatcta caatggtcat tgttggattg
     1621 gccggggttt ccaaataaat aagttttgta ttaggccgta tatgtttttc tacagattcc
     1681 agatcagaag tgtcgatcca gtcgctttcg acgccaaaac gtttgaaaat ggtttcggca
     1741 atcaagcgcg atgaaccata aacggcctca tgaccgacca tgtgctcgcc cgcaccgagt
     1801 aaacctgcgt aaattgtact tacagcaccc atgcccgaag ccgtggcaat acctccatat
     1861 cctttctcga gtatggctaa ggttttttcc aaatcggtaa tggtggggtt acccaaacgg
     1921 gtatagatat accccgattc ttcccccgca aaacatgctg ccccatgatc ggcattctcg
     1981 aaagtaaatg tggaagtttg atatataggc gtaatagcac tattcattcc attttcaact
     2041 cctccggcat gaaccaaaag agaattgaaa cctaattcct tattatccat aaattatcga
     2101 attttgctgc aaaggtaagg atttcatccc gtataactat tcattgtcaa tttaacttaa
     2161 tgaaacggaa gaggatgata ttcgccttat taagaattct catgtgatct actcaatata
     2221 ggattatgtg aatattgaca tttctccgtc tccttgactt ttcgtctcaa gcctcttcca
     2281 ttttccattt ctcgtaggca gttttctatc cgccattctc catgtctcac ctcgcacttc
     2341 gatatttttt ggccttttcg aaaacaagac caatgcattg ttttgttatt ttttagggtg
     2401 aatgccatta aggcggatta tagtatccac taaggctgta acaatagaaa gagcaatttc
     2461 ttcgggagtc tcggcggcta tgggtaaaca atcggaagat cgatttttat gatgagttct
     2521 ttatccagat aatatttttc ttgtaaggtt tttcgtgcct cccggctttt cgatttcgaa
     2581 cctgtcatgc caatataggc tggtcgtaaa gtggcgatgg ctgctgtgag agaaatatcg
     2641 agggcatgtt catgagtaac tactactaca aagtcatttg tattgatatc taattttgtg
     2701 atgatctcgc tgtaatttcc ctcgatg
//
LOCUS       DAORPD010000136         2724 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_6668_length_2724_cov_2.692019, whole genome shotgun sequence.
ACCESSION   DAORPD010000136 DAORPD010000000
VERSION     DAORPD010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6668_length_2724_cov_2.692019"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            217..972
                     /locus_tag="PK976_02320"
     CDS             217..972
                     /locus_tag="PK976_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HRC66753.1"
                     /translation="MLHNLCQVSDSLVIIPTYNEKENIERIIRKVFSLEPAFDILVVD
                     DNSPDGTARIVKQLIQEFPERLFIEERSGKLGLGTAYIHGFKWALEKDYQFIFEMDAD
                     FSHNPNDLIRLRQAVAENSADLAIGSRYISGVNVVNWPIGRVLMSYLASKYVRLITGI
                     KVHDTTAGFICYSRSVLQTINLDEIKFTGYAFQIEMKYSAWKLGFTIIEIPIIFTDRT
                     AGESKMSRGIFKEAALGVIKLRFKKIVPNKRQE"
     gene            1020..2360
                     /locus_tag="PK976_02325"
     CDS             1020..2360
                     /locus_tag="PK976_02325"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HRC66754.1"
                     /translation="MQKTLITGAKMINEGNIQEMDILIEEPYIASIMRAGTNAGKACV
                     IEAQGKYLLPGIIDEHVHFREPGLTHKADIYTESRAAVAGGVTSFMDMPNTIPQTTNH
                     KTLEWKLKRASKSSLANYAFYLGATNDNLNEILDIDPAMIPGVKVFMGASTGNMRIDD
                     EETLINIFSKAPCLIAVHCEDEDLIRKNLAYYQSIYAKDIPLEYHSLIRSADACQKST
                     QKAIALAREYDSRLHVLHLSSVEETKLFTNKLPSNQKQITSEVCIHHLWFSNIDYKEK
                     GTKIKWNPSIKTPADKKALWEALLDDRIDAVATDHAPHTLQEKQNPYLSCPSGAPMVQ
                     HSLSAMFEMAFQRKLELTRVVEKMCHAPALLFNIHKRGFIREGYFADLVLIDPNEPWE
                     VNSSNILYKCGWSPMEGTHFSSRVTHTWVNGHLVYKKGLFDESRKGMQLMFDNR"
     gene            2364..>2724
                     /locus_tag="PK976_02330"
     CDS             2364..>2724
                     /locus_tag="PK976_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66755.1"
                     /translation="MKKQIYFFWMLLAVLFTSCGRKTVNIVKEEFEDGSPKVVRQYEI
                     RGKDSILLRETIFYQNGQKYIEGKYTNNQRDSIWTAWLPDGKIWSQGGYKEGLEDGPK
                     KVFYTNGSLFYEGEHKMG"
BASE COUNT          903 a          541 c          558 g          722 t
ORIGIN      
        1 gcttcggtaa ttcaattgaa gctcgaagta ggttcgaagg tatgggtttc gttcaaggca
       61 tcatcggtcc aattcctcga aggataataa tacaaactat atataacagg aaacactaag
      121 atgcaaaaag aaagcagaat ataaaattac aaaagttgta aaagaaagtt gataagaatt
      181 tatttatagt tcgcataata agataattcc gtaattttgc tccataattt atgtcaagtg
      241 tccgatagtc tggtaatcat acctacatac aacgaaaagg agaacatcga gcggattata
      301 cgcaaggtat tttcgctcga acccgctttc gatatacttg ttgtggatga taattcaccc
      361 gacgggaccg ccagaattgt aaagcaactg atacaggaat ttcccgaacg gcttttcatt
      421 gaagaaagaa gtggaaaatt aggacttggc acggcttata tccatggttt caaatgggca
      481 ttggaaaaag attaccaatt cattttcgag atggatgccg acttttcgca caaccccaac
      541 gatctgatcc gtttacgaca agcagttgct gaaaatagtg ccgatcttgc cataggttca
      601 cgatatatat cgggagttaa cgtagtaaat tggcccatag gaagggtatt gatgtcatac
      661 cttgcttcaa aatatgtgcg cttgattacg ggtataaaag tccatgatac tactgccgga
      721 tttatttgct acagtcgcag cgtgctgcaa actatcaatc tcgacgaaat taaatttacc
      781 ggctatgctt ttcagatcga aatgaaatat tctgcatgga aacttggttt tacgattata
      841 gagattccca tcatctttac cgaccgaacg gcaggggaat cgaaaatgag caggggaatt
      901 ttcaaagaag cggcactggg agtcatcaag cttcgattca aaaagatagt tcccaataaa
      961 cgtcaggaat aattgtcttt gcacttttaa accttcattc aacggggaat cccatgaaaa
     1021 tgcaaaaaac gttgattacg ggtgccaaaa tgataaacga agggaatatt caggaaatgg
     1081 atattcttat cgaagaaccc tatatagctt ccatcatgag agccggtaca aacgcaggaa
     1141 aggcatgtgt aatcgaagct cagggaaaat atctccttcc cggaataatc gacgagcatg
     1201 tgcatttcag agaaccaggt cttacacaca aagccgacat ctataccgaa tcaagggcag
     1261 cagtagctgg cggagtaaca tctttcatgg atatgcccaa tactatccct caaaccacta
     1321 accataaaac acttgaatgg aaactgaaaa gagcttctaa gagttcactc gccaattatg
     1381 ccttttatct gggagctacc aacgataatc tgaacgaaat tctcgatatc gatccagcca
     1441 tgatacctgg cgtaaaagtc tttatgggtg cttctacagg taatatgcgg atagatgacg
     1501 aggaaaccct aatcaatatc ttctcaaaag ctccatgcct tatcgcagta cattgcgaag
     1561 acgaagacct catccgtaaa aaccttgcct attatcaatc tatctacgca aaagatattc
     1621 ccctagaata ccattctctt atccgctcgg ctgacgcttg tcagaaaagc actcaaaaag
     1681 ccatcgcatt agccagggag tatgatagcc gcctgcatgt tctccatctt tcttccgtcg
     1741 aagagacaaa actttttacc aacaaacttc cctcgaatca aaaacaaatt acctccgaag
     1801 tatgcataca tcatctttgg ttctccaata tagactataa agaaaaagga acaaagatca
     1861 agtggaatcc ttctataaaa actcctgcgg ataaaaaagc actatgggag gccttactcg
     1921 acgaccgcat tgatgcagta gccaccgatc atgcacctca tacacttcag gaaaaacaga
     1981 atccctatct gagttgtccg tcaggagcgc ctatggtaca acatagtctt tccgctatgt
     2041 tcgaaatggc atttcaacgt aaacttgagc ttacaagagt tgtggaaaaa atgtgtcatg
     2101 caccggctct actttttaat attcataaaa gaggttttat acgggagggt tattttgccg
     2161 atctcgtcct aatcgacccg aatgaaccat gggaggtaaa ttcaagtaat attttgtata
     2221 aatgcggctg gtcgcccatg gaaggaactc atttctcctc acgggttact catacctggg
     2281 taaatggaca cctggtttat aaaaaagggt tgttcgacga aagtcgaaaa ggaatgcaat
     2341 tgatgtttga taatcgttaa aaaatgaaaa aacaaatcta ttttttctgg atgttattgg
     2401 cagttctatt cactagctgt ggccgtaaaa ctgtaaacat tgtgaaggaa gaattcgaag
     2461 atggttctcc aaaggttgtg cggcaatatg aaattcgagg aaaagacagc attcttctcc
     2521 gagaaaccat tttttaccag aatggacaaa aatatataga agggaaatat actaacaatc
     2581 agcgcgacag tatatggact gcctggcttc ccgatggaaa aatttggagt caaggagggt
     2641 acaaggaagg cttggaagat ggacctaaaa aagtatttta tactaatggg tctctttttt
     2701 atgaaggtga gcataaaatg ggaa
//
LOCUS       DAORPD010000137         2723 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_6675_length_2723_cov_2.253373, whole genome shotgun sequence.
ACCESSION   DAORPD010000137 DAORPD010000000
VERSION     DAORPD010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6675_length_2723_cov_2.253373"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="PK976_02335"
     CDS             complement(<1..560)
                     /locus_tag="PK976_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66756.1"
                     /translation="MKHLFPLLSLVLLVSLFNIGCSKDEDEELPKDLVVNYTASFTDV
                     ASTLQDFIMKAQVYESSNFESLTADQGKKIINDYITAGENFIKSVDDYEKYKIGTKNS
                     SPWLKNSMDSPCSMYDAIPDVGNGISPGFVKSVGDLIADTKGDVKKIEKKFNDGEISE
                     EVFTAALDSLKKIKTLKAANFGMSSI"
     gene            complement(1033..2040)
                     /locus_tag="PK976_02340"
     CDS             complement(1033..2040)
                     /locus_tag="PK976_02340"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRC66757.1"
                     /translation="MQNKVTIKDIAKTLGVSISTVSRALKDHPDISESTRNLIKNFAQ
                     EINYRPNVLALSLRRQKSNTIGLIIPEIVHHFFSSVISGMEDLSYREGYNIIICQSTE
                     NQFREATALQTLIDHRVDGILVSLSKSTQNFSQFKKAQEEGFPMVFFDRICESIETDR
                     VVMDDLEGGRLATEYLINKGCQNILHLAASPILLIGRNRRDGYLQALKDHNIPIKEEL
                     ILKCDTQEEVSTLRNHLYELLPQIDGVFAVNDSTAIAMIQILKKKGYRIPQDIKVVGF
                     GDGPNATIVEPALTTIEQKGYDMGRESVRLLLQRLNNPSASISYQTRVFPPTLKIRES
                     A"
     gene            2369..>2723
                     /locus_tag="PK976_02345"
     CDS             2369..>2723
                     /locus_tag="PK976_02345"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRC66758.1"
                     /translation="MMKHANLLKVLFLFFFAAVALPSFAQQLTVKGKVTDASDGTTLP
                     GVTIVVKNTTTGTVTDINGNYSITVPKGSTLAFSFVGYQTQEFVIETSQTLNVALSPT
                     ITSLSQILVIGYGQVK"
BASE COUNT          826 a          488 c          498 g          911 t
ORIGIN      
        1 ataatggagc tcatcccaaa attcgctgcc ttcaatgttt ttattttctt taacgaatcg
       61 agagccgccg taaaaacttc ttcacttatc tcgccatcgt taaatttttt ctcaatcttt
      121 tttacatcgc cttttgtgtc agctatcaaa tcaccaacac tttttacgaa tcctggtgat
      181 attccattcc ccacatcagg gatagcatcg tacatgctac atggtgaatc catcgaattc
      241 tttaaccatg gggaagaatt tttggtgcca atcttgtatt tttcataatc atccacactc
      301 ttgatgaaat tttcaccagc tgtaatgtaa tcgttgatga tcttcttgcc ttgatcggca
      361 gttaatgatt caaaattaga actttcatac acttgtgctt tcatgataaa atcttgcagt
      421 gtagaagcta cgtcggtaaa actggcagtg taattaacta ccagatcttt gggtaattct
      481 tcatcttcat ctttactgca gcctatgttg aataagctaa caagtaaaac taaactgagc
      541 aaaggaaata aatgtttcat agttgttaag gattttggat taaacttaag gttgatttta
      601 aaaatgtggt tcgatgactt ttaattatga atgatgtaag atagatgtaa tacatgagaa
      661 atcacaaagt aatttataag atgttaaact gaaagaatac aatagaatgg tttttgaatc
      721 aggtttgata tttattaaca aaggtataca taaaaatcac ttttttcaaa gaaaacaaga
      781 atttttttat gtagaaattt tcaataatgg ttgaatgaac aatttatgtg agaaatcata
      841 ggtaaaaaaa agaaattttt caggcatcca ccttccattg attttatcac aaatccttcg
      901 ttctatgaaa tgatagaatg caagctctaa tgattcatct tgcttaatta tttcatattc
      961 agtcttttat tcattgtttt gttttatttt taaggggcta ttctgtcgag aattatgggc
     1021 gcaaagaatt catcaggcag actctctgat cttgagtgta ggagggaaaa cacgggtttg
     1081 ataactgatg gaagccgatg gattgttgag gcgctgcagg agcagtctta cggattcacg
     1141 gcccatgtcg taaccttttt gttctattgt ggtgagggcg ggttctacta tagtagcatt
     1201 aggtccatcg ccaaaaccta ctactttaat atcttgagga attcgatagc cttttttttt
     1261 cagtatttga atcatggcga tggcagtact gtcatttacc gcaaaaaccc catcaatttg
     1321 cggtaaaagt tcataaagat ggtttctaag ggttgaaact tcttcctgag tatcacattt
     1381 caggataagt tcttctttga ttgggatatt gtgatctttc aatgcttgga ggtatccatc
     1441 gcggcggtta cgtccaatca gtaaaatggg cgatgccgcc agatgaagta tattttggca
     1501 gcctttattg atcaggtatt cggttgccaa gcgacctcct tctagatcat ccatgactac
     1561 tcgatcggtt tctatacttt cgcaaattct atcgaaaaat accatgggga aaccttcttc
     1621 ctgtgccttc ttgaattgag aaaaattttg tgtggatttg ctcagtgaaa ccaatattcc
     1681 atccaccctg tgatcaatca aagtttgaag tgctgtagct tctcgaaact gattttctgt
     1741 cgattggcaa ataatgatat tgtatccttc acgatagctg aggtcttcca tgccactaat
     1801 caccgatgaa aaaaaatgat gaacgatttc gggaatgatc agtccaatgg tattgctttt
     1861 ttgtcgcctg agactcaaag ccaaaacatt agggcgatag ttgatttcct gcgcaaaatt
     1921 cttaatgaga tttctagtcg attcactgat atcgggatga tctttcagtg ccctggatac
     1981 cgttgatata gaaactccca atgttttggc tatatctttg atagtaactt tattttgcat
     2041 aataccctga cttttaattg cttaataatt ttatgccgct tccttcgatg atttatcgaa
     2101 cgatttatac gcggccaacg aaaataattc aataattttt caagcgcaaa cgtttgcgaa
     2161 atcgttttcg caaaaaaaaa ctcaaaaatc tcaaaatcag tgaaacatat ttgcaattga
     2221 aaacgaaaaa agttgtatat ttggttcggg aaaaagttaa aatcgaacca tctgttttca
     2281 gtagtttttt catcgaaaat gaaagaatat tctgaaaaga ggtgttaacc aaatcattta
     2341 atgttaaacc ataactaaaa ccagatgtat gatgaaacat gctaacttac tgaaggtact
     2401 gtttcttttc ttttttgcgg ctgttgctct gccttcgttt gcgcagcagc ttacagtaaa
     2461 ggggaaagta acggatgctt ctgatggaac cacgctacca ggagttacca ttgtagtgaa
     2521 aaacactact acaggtacag ttactgacat caacggtaat tacagtatta ccgtacctaa
     2581 gggatctact cttgcttttt catttgtagg ctatcaaact caggagtttg tgattgaaac
     2641 ctcacaaacc cttaatgtag ctctatctcc aaccatcacc agtcttagcc agatattggt
     2701 tataggatat ggacaggtga aaa
//
LOCUS       DAORPD010000138         2715 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_6698_length_2715_cov_2.543233, whole genome shotgun sequence.
ACCESSION   DAORPD010000138 DAORPD010000000
VERSION     DAORPD010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6698_length_2715_cov_2.543233"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1016
                     /gene="thiL"
                     /locus_tag="PK976_02350"
     CDS             <1..1016
                     /gene="thiL"
                     /locus_tag="PK976_02350"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010993338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HRC66759.1"
                     /translation="SELGEFDLIDRLTEGLTPLNHSTIKGVGDDAAVMQYDNHSLILL
                     ANDILIEGVHFDLTYTPLKHLGYKAGVVNFSDIYAMNGAPKQLVIGLGVSNRFSVEAL
                     DEFYEGLRLACHHYGVDLVGGDISSSKTGMFISISVVGEVSKDEVVYRNGAQLDNLIC
                     VSGDLGAAYMGLLLLEREKRVYLANPNMQPDLEGNDYVLERQLKPEARKDIIRLLHEA
                     CIQPTAMIDISDGLASEILHICKQSNKGCVIYEDKIPIDPATLLLCDEFKIIPAIAAL
                     HGGEDYELLFTIPLSEYEKIEKVENISLIGHITDSSEGCQLITNDGKQIPLQAQGWNH
                     LRK"
     gene            1036..>2715
                     /gene="uvrC"
                     /locus_tag="PK976_02355"
     CDS             1036..>2715
                     /gene="uvrC"
                     /locus_tag="PK976_02355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HRC66760.1"
                     /translation="MTKSDEQINEHLSQILQTLPHSPGVYQFFDENQKIIYIGKANDL
                     KKRVSSYFTKETGVSGKVLVMVRRIRSIEVIVVDTELDALLLENNLIKEHQPRYNINL
                     KDDKTYPWLCIKNEPFPRIFPTRNPVKDGSKYYGPYASGRIMNNLLELIHQIYPLRNC
                     NLKLTFRNIEKGKFKPCLEYHIGNCKAPCIGLQTEEEYDANIASIKDIIRGNLSSVAS
                     SLKREMLAHAENLEFEKAQRVKEKLELLEKYQSKSAVISNTDLHADVFSIVSDEKIAC
                     VNFMKVANGAVIQSHTIEIRKKLDESDEEILSFAITELRLRFESQVREIIVPFKLTID
                     FPGILITVPKKGDKRQLLELSLSNAEYYRLEKLKRIELVDPERHSKRILHQIMKDLRM
                     NQLPEHIECFDNSNLQGTEAVAAVAVFRNAKPSKSEYRHFNIKTVEGQNDFASIEEIV
                     YRRYKRLLEEGEGLPQLIVIDGGKGQLASALFSLEKLGLKGKITIIGIAKRLEEIYYP
                     GDSLPMYLDKKSETLRLLQHIRDEAHRFGITHHRKRREKAFIQTELTSIRGI"
BASE COUNT          862 a          526 c          593 g          734 t
ORIGIN      
        1 tatcagaatt gggtgaattt gatttgatcg accggttgac cgagggtttg actcccctca
       61 accatagcac tataaaaggg gttggagatg atgcagccgt tatgcaatat gataatcatt
      121 cgcttattct tcttgctaat gacattctaa tagaaggtgt acattttgat ttaacttaca
      181 ccccattaaa gcacttagga tataaggcgg gagtagtaaa tttttcggat atatatgcta
      241 tgaatggtgc acctaagcag ttggtcattg gcttgggtgt atcgaatcgc ttttcagtag
      301 aagctctcga tgagttctat gaaggcttgc gtcttgcttg ccaccattat ggagtggatt
      361 tggtgggagg cgacatttcg tcgagcaaaa cagggatgtt tatttcaatt agtgtagttg
      421 gcgaagtttc aaaagatgaa gtggtttacc gcaatggagc ccaacttgac aatcttatct
      481 gtgtttcggg ggatctggga gcagcttata tgggattatt gctcttagaa cgcgaaaaac
      541 gggtttatct ggccaatccc aacatgcaac ccgacctcga aggcaatgat tatgttttgg
      601 aacgtcaact caaaccagag gcacgtaaag acatcattcg tcttttgcat gaagcctgta
      661 ttcaacctac agccatgatc gacatctcag atggtttagc aagcgaaatt cttcatattt
      721 gcaagcagtc caataaaggt tgcgtaatct acgaagacaa aattcccatc gatccggcta
      781 cactactatt atgcgatgag ttcaaaatca ttcctgctat tgctgccttg catgggggag
      841 aggactatga actgttattt acgattcctc tgtcggagta tgaaaaaatc gaaaaagtgg
      901 aaaacatctc acttattggc catattaccg attcatcgga aggatgccag ttaattacca
      961 atgatggcaa acaaatcccc cttcaggctc agggttggaa tcatttgaga aaataattcc
     1021 ttcaaatgtt gcagcatgac aaagagtgac gaacaaatca acgagcattt atcgcaaatt
     1081 ctacaaaccc tccctcatag ccctggagtg tatcagtttt ttgatgaaaa tcagaaaatt
     1141 atctatatcg gtaaggcaaa tgatttgaag aaaagagttt cttcatattt tactaaagaa
     1201 actggggtaa gtggtaaagt attagtgatg gtgaggcgta tcagaagcat cgaagtgatt
     1261 gtggtagata ccgagttgga tgcacttcta ctcgaaaaca atctgatcaa ggagcatcag
     1321 cccaggtata atatcaatct gaaagacgac aagacatatc cctggctttg tataaaaaac
     1381 gagcctttcc cgagaatatt tcctacaaga aatccagtaa aagatgggtc gaaatattat
     1441 ggtccttatg cctcgggacg aatcatgaac aacctcctgg agttgattca tcagatttat
     1501 cctttacgca actgcaacct gaaacttacc tttcgaaata tcgaaaaagg aaaatttaag
     1561 ccctgtcttg agtatcatat cggaaattgc aaagctccct gtattggact gcaaaccgaa
     1621 gaagaatacg atgccaatat tgcttcgatt aaggatatta ttcggggaaa cttatcatct
     1681 gtggcttcgt ctttgaaaag agaaatgtta gcccatgccg aaaatctcga gtttgaaaag
     1741 gctcaaagag taaaagaaaa attggagctg ctcgaaaaat accagtcgaa atcggctgta
     1801 atcagcaata ctgatttaca tgcagatgtg ttttcgattg taagtgacga aaaaatagct
     1861 tgtgtcaatt ttatgaaagt agccaatgga gcagtcatcc agtcgcacac catagaaatt
     1921 cgcaagaaat tggacgagtc cgatgaggaa atactgagtt ttgccattac tgaactcagg
     1981 ttaagattcg aaagccaggt tcgggaaatt attgttcctt tcaaattgac cattgatttt
     2041 cctggcattc tgattacagt gcccaaaaaa ggagataaaa ggcagcttct cgaactttcg
     2101 ctgtcgaatg ccgaatacta tcgtttggag aaactcaagc gcattgaact ggtcgaccct
     2161 gaaagacata gcaaacgcat tctgcatcaa ataatgaaag atttgcgtat gaatcaattg
     2221 cccgaacata tcgaatgctt cgacaacagc aatttgcaag gcactgaagc cgttgctgcc
     2281 gtggcagtat tccgcaatgc aaaaccctct aaaagcgaat atcggcattt caacatcaaa
     2341 accgtggaag gtcaaaacga cttcgcttcg atagaggaaa tcgtttaccg aagatacaaa
     2401 cgccttttag aagaagggga agggcttccc cagctgattg tgatagatgg tgggaaaggg
     2461 cagcttgcat cggcactctt cagtcttgaa aaattgggac tcaaaggcaa gatcaccata
     2521 ataggtattg ccaaacgttt agaagaaatc tattatcccg gagattcgct tccaatgtat
     2581 ttagataaaa aatcggaaac tctgaggctg ttgcagcata tccgcgacga agcccatcgt
     2641 tttggaatta cacatcatcg caaacgaaga gaaaaggcat tcatacagac tgaactcaca
     2701 agtatcaggg ggatt
//
LOCUS       DAORPD010000139         2711 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_6709_length_2711_cov_2.180346, whole genome shotgun sequence.
ACCESSION   DAORPD010000139 DAORPD010000000
VERSION     DAORPD010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6709_length_2711_cov_2.180346"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..709)
                     /locus_tag="PK976_02360"
     CDS             complement(<1..709)
                     /locus_tag="PK976_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66761.1"
                     /translation="MAILCLTACKDKEEYRFDTSYNKQAISDFLTADAIYRDLFRQAY
                     QLSFDTSILKKGEGIIDSAIVKKQGEEWIFTYDKEQNCPDGSKRSGQFRIHWNAPLSD
                     SNAQLTIFPQGLTINEKRIGGQINITRISKPKVFYRVKVDTGSISLVVAGYSKKQRIQ
                     YSSNYKYYWQKGSQTPYDIYDDVLYMYGIASGRGREFDRFIYVTLDTLTYRFICHYPR
                     GGKALFQMPDFEINQMDL"
     gene            complement(794..1291)
                     /locus_tag="PK976_02365"
     CDS             complement(794..1291)
                     /locus_tag="PK976_02365"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HRC66762.1"
                     /translation="MEKKPLTIIVAIANRNTIGLNNQMLCHLPDDLKRFKRITMGHNI
                     IMGRKTWESLPKKPLPGRNNIVLSRRVLQLEGATVVSSLEQALDLCPLNEESFVIGGG
                     ALYRLFLPYVDKLYITRIYHDFEADTFFPDFDEKEWQLVMQEDVFPNETNPYRYSYLT
                     YNRKH"
     gene            complement(1292..2086)
                     /locus_tag="PK976_02370"
     CDS             complement(1292..2086)
                     /locus_tag="PK976_02370"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011203420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HRC66763.1"
                     /translation="MKQYLELLRHILATGTEKHDRTGTGTISVFGYQMRFNLEDGFPL
                     LTTKKLHVRSIIYELLWFLRGDTNIRYLHENKVTIWDEWADANGDLGPIYGYQWRSWP
                     TREGKSIDQISQVIDSIKQNPDSRRHIVSAWNVEEIDKMALPPCHIFFQFYIANNKLS
                     CQLYQRSADVFLGVPFNIASYALLTLMTAQITGYQPGELIHTLGDAHIYKNHIEQAKL
                     QLTREPFPLPTMILNPEIHSIFDFRYEDFQLINYRAHPHIKAEISV"
     gene            complement(2096..>2711)
                     /locus_tag="PK976_02375"
     CDS             complement(2096..>2711)
                     /locus_tag="PK976_02375"
                     /inference="COORDINATES: protein motif:HMM:NF012922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HRC66764.1"
                     /translation="FFHYHWNDYIMMKRKPFALISFLLIVISACIRPQYDNFSSIGED
                     HYLRMVIETPAGNLNCVHFHPSSNSFIQGNAFQKPAPLNFGFIPSSNRSKSAQEFDPI
                     RVMLLSNSKPSGTILKIIPIGMLQFAASGKQRVLILAVPADPSHRKSNINDEAAISMT
                     EPSIFSILELWLKNYSAIPDVIIYGWADENITWNYILDVLRLKS"
BASE COUNT          808 a          527 c          556 g          820 t
ORIGIN      
        1 caagatccat ctggtttatt tcaaaatcgg gcatctgaaa taatgccttt cctcctcttg
       61 gataatgaca tataaatcgg tatgtcaaag tatcgagagt tacataaata aaacgatcga
      121 actctcttcc ccggcccgat gctatgccat acatgtacaa aacatcgtca taaatgtcat
      181 aaggcgtttg agatcctttc tgccaataat atttataatt actactatac tgaatccttt
      241 gctttttcga ataaccagcc acgaccagcg agatagaacc cgtatcgact tttactctgt
      301 aaaaaacctt gggtttactg atacgtgtaa tgttgatctg cccgcctatt cgcttttcat
      361 tgatggttaa accttgtgga aaaatggtaa gctgtgcatt ggaatcgctc aaaggtgcat
      421 tccagtgtat ccgaaattgt ccgcttcgct tcgaaccatc agggcaattc tgttctttat
      481 cgtacgtaaa aatccattcc tctccctgtt tctttactat agccgaatcg ataatccctt
      541 cacccttttt caatatagaa gtatcgaacg ataactgata agcctggcgg aacaaatcgc
      601 gataaattgc atcggccgta agaaaatcac ttatggcttg cttgttataa gaagtatcga
      661 acctatattc ttctttatcc ttgcatgccg ttaaacatag aatagccatt gcgattagaa
      721 tgagaatttt atcctttcta aagcaaagaa tgaatttcca tgaatggcac gaaaagtgaa
      781 gcatagcatg aatttaatgt tttctgttat aagttaaata cgagtatcta tatggattgg
      841 tttcgttagg aaaaacatct tcctgcatta cgagttgcca ttctttttcg tcgaaatcgg
      901 ggaaaaaggt atcagcttcg aaatcgtgat agattcgagt gatatagagt ttatcaacat
      961 aaggcagaaa aagtcgatag agagccccac ctcctatgac gaaactttcc tcatttaaag
     1021 ggcataaatc cagtgcctgt tcgagggaag agaccacagt agctccttcc aattgaagta
     1081 ctcgacggct tagaacaata ttgttgcggc caggtaaggg ctttttgggc agtgattccc
     1141 acgttttacg acccattatt atgttatggc ccatggtaat ccgtttgaaa cgtttcagat
     1201 cgtccggcag atgacagagc atctggttat tcaggccgat ggtattgcga ttggcgattg
     1261 caactataat agtgagaggt ttcttttcca tctaaaccga tatttcagct tttatatggg
     1321 gatgagctcg ataattgatc aattgaaaat cttcatatcg aaagtcgaaa atagaatgaa
     1381 tttctggatt caaaatcata gtaggaagtg ggaagggttc tcgtgtaagc tgcagttttg
     1441 cctgttcgat atgattttta tatatatggg catcacctag ggtatgaatc aattctccag
     1501 gttggtatcc cgttatctga gccgtcatca gggttagcaa ggcatacgaa gcaatattga
     1561 agggaactcc aagaaataca tctgcacttc gctgatagag ctggcaactc agtttattgt
     1621 tagcgatata aaactggaaa aatatatggc agggaggcaa ggccatttta tcgatttctt
     1681 ctacgttcca tgcactaacg atatgccggc gagaatcggg attttgcttt atcgaatcaa
     1741 tgacttgact gatttggtcg atggatttgc cctctctggt aggccatgat cgccattgat
     1801 aaccataaat tggtccaaga tcaccatttg cgtcggccca ttcatcccag atggttacct
     1861 tattttcgtg caggtatcga atattggtat ctccacgtaa aaaccagagc agttcataaa
     1921 taatactgcg gacatgaagt tttttagtag taagcaatgg aaatccatct tcgagattga
     1981 aacgcatctg ataaccaaac acgctgattg ttcccgtacc cgtacggtca tgtttttcag
     2041 ttccggttgc gagaatatga cgaagaagtt cgagatattg tttcatagat tcactttaag
     2101 attttaggcg aaggacatcc aaaatataat tccaagttat gttttcgtcg gcccatccat
     2161 atatgataac gtcggggata gcagaataat ttttcagcca taattcgaga attgaaaaaa
     2221 tagaaggttc agtcatgctt attgcagcct catcgtttat attggatttt ctgtgggaag
     2281 gatcggccgg gactgcaagg atcaaaactc tttgctttcc actggccgcg aattgcaaca
     2341 tgccaatggg aataattttc aatatggttc ccgaaggttt agaatttgat aacagcatta
     2401 ctcttatagg atcgaattct tgagccgact tcgatctatt ggacgatgga atgaagccaa
     2461 aattaagagg cgcaggtttc tgaaaagcat ttccctggat aaaactgttg ctcgaaggat
     2521 gaaaatgaac gcaattaagg ttcccggctg gagtttctat caccattctc aaataatgat
     2581 cctcgcctat ggacgaaaaa ttgtcatact ggggccttat acaagcacta atgactatta
     2641 gaaggaatga aattaaagcg aaaggctttc tcttcatcat aatgtaatca ttccaatgat
     2701 agtggaagaa a
//
LOCUS       DAORPD010000140         2705 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_6729_length_2705_cov_2.150189, whole genome shotgun sequence.
ACCESSION   DAORPD010000140 DAORPD010000000
VERSION     DAORPD010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6729_length_2705_cov_2.150189"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1155)
                     /locus_tag="PK976_02380"
     CDS             complement(<1..1155)
                     /locus_tag="PK976_02380"
                     /inference="COORDINATES: protein motif:HMM:NF036484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HRC66765.1"
                     /translation="MIPASHIQDLDYHQYILIKGARQHNLKNFDLAIPKDKLVVITGL
                     SGSGKSSLAFDTLFAEGQRRYIESLSAYARQFIGRMSKPDVDFIHGIAPAIAIEQKAN
                     TRNPRSTVGTSSEIYEYLKLLFARIGKTYSPISGNIVKRDTPTDVTDFIAKLPQGSRS
                     ILLSPIHQNSGENLSSRLNIFLQQGFTRVSINGNIFHIEEILSSQNIIDSNAEIQLVI
                     DRFTGGDIDEEFVSRIADSVEIAFYEGMGTCMVDYWQNGELHRQIFSNRFEADEIAFE
                     EPSVNLFTFNNPYGACKTCEGFGSVIGIDEDLVIPDKSLSVYEECVAPWKGEKMGEWR
                     WQFIQAARSRGFPIHKPYDELNEEQKQLLWKGDRGILGINDFFEMVQENLY"
     gene            1470..2054
                     /locus_tag="PK976_02385"
     CDS             1470..2054
                     /locus_tag="PK976_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008123213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRC66766.1"
                     /translation="MNYTALSDQELIKNYRSGDSDSLEHLILRHQEKVTSYVLNVVKN
                     RHLADDIVQDTFLKVINTIRSGAYKEEGKFIQWVMRIAHNLMIDYFRRQKKFPIAEPG
                     EGMDIFSTLPLQDRSVEDAIIYEQILTDVKRMLKYLPEEQRRVVEMRLYEDLSFKEIA
                     EITKVSINTALGRMRYALINLRKIAKENDIQLSM"
     gene            2517..2681
                     /locus_tag="PK976_02390"
     CDS             2517..2681
                     /locus_tag="PK976_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66767.1"
                     /translation="MDKNSTHEIQQKFLFKRKHFVPMPGGRLAFLMAYSHALLVVSTR
                     MIGDCFINMN"
BASE COUNT          818 a          506 c          559 g          822 t
ORIGIN      
        1 gtaaagattt tcctgtacca tttcgaagaa atcatttatg cctaatattc ccctatcgcc
       61 tttccagaga agttgttttt gttcttcgtt caattcatca tagggcttat gaatggggaa
      121 accacgtgaa cgcgctgcct ggatgaactg ccaacgccat tctcccattt tttctccctt
      181 ccatggagcc acacattctt cataaaccga taaactcttg tcggggatga ctagatcttc
      241 atcgatacct atgactgaac cgaagccctc gcaggtttta catgcaccat aaggattgtt
      301 gaatgtgaat agattgacgc ttggctcttc gaaggcaatt tcatcggctt caaaacgatt
      361 ggaaaagatt tgcctgtgaa gttcgccgtt ttgccaataa tcgaccatgc aagtacccat
      421 gccttcgtaa aatgctattt ctactgaatc ggctatcctc gaaacgaatt cttcgtcgat
      481 gtctcctcct gtaaaacgat caataaccaa ttgtatttcg gcgttggaat cgatgatatt
      541 ctgcgaagaa aggatttctt cgatgtggaa aatatttccg tttatcgaga ctcgggtaaa
      601 accttgctgg agaaagatat tcaagcgtga agagaggttt tcgccagaat tctgatggat
      661 tggcgaaagt agtatgctgc gtgaaccttg aggtagtttg gcaataaagt cggtaacatc
      721 ggtgggagta tcacgtttaa cgatgttccc tgaaatgggt gaataagttt tcccaatgcg
      781 agcgaaaagg agcttcaaat attcgtagat ttctgaggag gtacctacag tactgcgtgg
      841 attacgggtg tttgcctttt gttcgatggc aatggctggc gcaattccat gaataaaatc
      901 tacatcgggc ttgctcatcc gaccaatgaa ctgacgtgca taggcgctaa ggctttctat
      961 atagcgtcgc tggccttcag caaaaagggt atcgaaagcc agagatgatt tacccgagcc
     1021 tgaaagcccc gtaatcacta ctaatttatc ctttggaatg gctaaatcga agtttttcag
     1081 attatgctgc cttgctcctt ttattaatat atattgatga taatcaaggt cttgaatatg
     1141 ggaagcaggt atcatccatg taacaaatta aatgtgcaaa attaatacta attcataggg
     1201 gatgctcctt agaaccctct ttccaaaaaa gatgaaagga ttataattta ttttattact
     1261 ggtaattaac cccctttttg aggtaaagaa actataaaag cagtaaaaat ttgaaaaaag
     1321 attgcttttt ttaaaaaatg atataaattt gcaccattga ttgaaagtgt cataaagaaa
     1381 gaatttgatt gttgaattaa aaacggaggc tgcctatcgc tgaacttcta cacttattga
     1441 aaccacgaac cctttagaag cgcagcgtta tgaattacac agcactttcc gaccaggaac
     1501 ttatcaagaa ctaccggtcg ggcgattcgg atagccttga acatttaatc ctcagacatc
     1561 aggaaaaggt tacttcatat gtgttaaatg ttgttaagaa tcgtcatctt gccgatgata
     1621 tcgtacagga tacctttctg aaagttatca ataccattcg gtcgggtgct tacaaggagg
     1681 aaggcaagtt tattcaatgg gtgatgcgca ttgcacataa tctgatgatc gattatttcc
     1741 gccgtcagaa aaaattcccc atagccgaac ctggagaggg aatggatatt ttcagtacct
     1801 tgcctttgca ggaccgatcg gtggaagatg ctatcatcta tgagcaaatc cttaccgatg
     1861 taaaaagaat gctgaaatat cttcccgaag aacagcgcag agtagtagag atgcgacttt
     1921 acgaagattt aagcttcaaa gaaatcgccg aaataacaaa agtaagtatt aatactgccc
     1981 tgggacgaat gcgttatgcc ttgatcaatc ttcgtaagat tgccaaagaa aatgacattc
     2041 aactcagcat gtgaggaatc cacattgctt ttaaatctca tatcatccag cgtttataaa
     2101 taaaggggaa agagaatcct gttgggttaa actttaatct gtaatgctct atattaggag
     2161 cagtgaggtt gttggattag tttatgaact attttggggg tttcatccat ctttcccctt
     2221 gtatagcctc atcttgaact tgaatatgat tagatacggg aaggaaatct ttaaggtggt
     2281 ttctccattt gtctttttgt aagcattcac atctctttct aaggttttat tcaaaaatag
     2341 gcttcccttc aaaaatgaga tcatccgtta aatccgggaa aattatgaat gcgatgctca
     2401 tgatgatgtt tgacgtgcgt atagttcttc gaataaattc tttttaagga agatatacta
     2461 aagccatagg cttagcgttt gaatagataa ttcaaagttt caccaaaatg caatgtatgg
     2521 ataaaaattc tactcatgaa attcagcaga agtttctgtt caagagaaaa cactttgtgc
     2581 ctatgccggg agggagacta gcatttttga tggcttattc gcatgctttg cttgtagttt
     2641 caacacgtat gataggcgat tgtttcatca acatgaacta aaacttttag ctaatccgaa
     2701 agggg
//
LOCUS       DAORPD010000141         2703 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_6737_length_2703_cov_2.464879, whole genome shotgun sequence.
ACCESSION   DAORPD010000141 DAORPD010000000
VERSION     DAORPD010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6737_length_2703_cov_2.464879"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(221..841)
                     /locus_tag="PK976_02395"
     CDS             complement(221..841)
                     /locus_tag="PK976_02395"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRC66768.1"
                     /translation="MLRDNIINTSVKLFRSMGIRSVTMEMVADEIGISKRTLYEHFKT
                     KDELVETCVHSELNRLIGKDTSIIERSSHIIETYLLFMWSHIQELRQTNYLFIKDLRQ
                     LYPSSLYCMTTEFESRIKEGIKNFIEKGKGQNIFRPEINVELATIILFEQLQMLMENS
                     EKIFSMHYFSPAEVFEHISINFIRGMATKEGVELIDHYYNEHQLTN"
     gene            complement(1074..2243)
                     /gene="rffA"
                     /locus_tag="PK976_02400"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(1074..2243)
                     /gene="rffA"
                     /locus_tag="PK976_02400"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR02379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HRC66769.1"
                     /translation="MHIPFNKPYFGPREAFATLGVALSGQFSGNGKYTQRCQTFFEKR
                     FHFAKTLLTTSCSDALEMAAILSEVGPGDEVIMPSFTFPSTANAFLLRGAKIVFADTL
                     PDIPNIDPKAIENLITPKTRAIVVVHYCGISCDMEAIQQLANKYGLLLIEDAAHSIDS
                     YYKGKPLGSFGHLAAFSFHETKNIISGEGGLLVVNDEQFTKRAEIIWEKGTNRAAFQR
                     KEVNRYEWVDIGSSFLPAEMVAAFLYAQLSKFDRIQKLRKKVWLSYYEYLKPLEEKGF
                     LHLPIVPEYASVNGNFFYIAVKDGYTRDELLRYLQSRGIHAVFHYLPLHQSPFFTFRH
                     DGRALPHTEKFSSTIIRLPFYPRLSKRKIKFVCNAISSFFESPQAYPVMVDHQMK"
     gene            2325..>2703
                     /locus_tag="PK976_02405"
     CDS             2325..>2703
                     /locus_tag="PK976_02405"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HRC66770.1"
                     /translation="MAKHSLIRDISGVMGSNILAMVSTLGISILLSRILGPQGYGLFT
                     SLYVVPQVVMGLVQLGMSRSAVFHLGHKKFNECQTISSIYVMLCMTSTVGIIVSAIAY
                     AILNNPLLETNWILLALCIIPFAL"
BASE COUNT          767 a          520 c          535 g          881 t
ORIGIN      
        1 tcgagtgagg aaacctgctg taagaggttt tgctcgatag ccctgtcaac agcttgttga
       61 agcgttaacc tcaaggtatc gttttggctc tttaattcta tgctgcaaag taaaaagaaa
      121 ccgataaaca ggcttcccca aagaatcatt tttaagttta ctttcatatc tcgtgtctat
      181 tgtttatatt ctgatattgg ataatctctt ttgaaatggt ttaattggtt agctggtgct
      241 cattgtaata atgatcaatt aattcgactc cttctttggt agccatcccc cgtatgaaat
      301 tgatggaaat gtgctcgaat acttcggcgg gtgaaaagta atgcatcgaa aatatttttt
      361 cactattttc cattaacatt tgcaattgtt cgaataaaat aatcgttgct aattctacat
      421 ttatttcagg cctgaagatg ttctgccctt tccccttttc aatgaaattt tttatccctt
      481 ccttgattct cgattcaaat tcagtagtca tgcaatagag gctgctcgga taaagctgcc
      541 tcaagtcctt tataaataga taattggttt gacgtaattc ctgaatatga ctccacataa
      601 acaaaaggta ggtttctata atgtgagaac tcctttcaat aatagaagtg tcttttccta
      661 tcagccgatt aagttctgaa tgtacacagg tctccaccaa ctcgtccttc gtcttgaaat
      721 gttcatataa agtacgtttc gatataccga tctcatcagc aaccatttcc atggttacgc
      781 tccgtatacc catgctcctg aaaagcttga ctgaagtatt gatgatattg tctcttaaca
      841 tttttaccca tttgaaaact taaaaaactt tttaagtttt cgacgtgcaa atgtataaca
      901 aatattctta caaattgctc gtaaaaataa aatttcaaga aaatttaaca tattctaata
      961 tccgtccaat ttttatgtta gagaatattt tttggttttt aactcaaaat caatctttta
     1021 ttcaagaatt catgaatgag ggataacaga tggatgattc catgaatttt ttttcatttc
     1081 atctgatgat ctaccatgac aggatatgct tgcgggcttt cgaagaagga agaaatagca
     1141 ttgcaaacga atttgatctt tcttttcgag agccttggat aaaagggaag acgaatgatt
     1201 gtggacgaaa atttttcggt atgcggcaga gctcgtccgt cgtgtcggaa agtaaaaaat
     1261 gggctctgat gaaggggtaa ataatggaaa acggcatgta tgcctctgct ttgcagatat
     1321 ctaagcaatt cgtctcgagt gtaaccgtct ttcaccgcga tatagaagaa atttccattt
     1381 acgctggcat attcaggcac gatggggagg tgtaaaaatc ctttttcttc taaaggctta
     1441 aggtattcat aatatgaaag ccatactttc ttacgaagct tctgaatgcg atcgaatttg
     1501 cttaactgtg cataaagaaa agcggctacc atttcggctg gcaaaaaaga agaaccaata
     1561 tctacccatt cgtatctatt gacttcttta cgctggaaag cagctctgtt ggtacctttt
     1621 tcccatataa tttcggctcg tttcgtaaat tgctcatcgt ttacgaccaa taagccacct
     1681 tctcccgaga tgatgttttt tgtttcgtga aatgagaagg ccgctagatg gccaaagctc
     1741 cccaagggtt ttcctttata ataggaatcg atggagtgag ctgcatcttc tattaataaa
     1801 aggccatact tgtttgccaa ttgctgaatg gcctccatgt cgcaggatat gccgcaataa
     1861 tgaaccacca caattgcgcg tgtttttggc gtaatgaggt tttctatggc ttttggatcg
     1921 atgttgggaa tatcaggtaa ggtatcggca aagacgattt tggctcccct cagtaggaag
     1981 gcattggcgg tggaaggaaa tgtaaacgaa ggcatgatga cttcatcgcc tggcccaact
     2041 tccgaaagaa tggctgccat ttccaaagca tcgctgcatg aagtggtaag taaagtcttt
     2101 gcaaaatgaa acctcttttc aaaaaatgtc tgacaccttt gggtgtattt cccattaccc
     2161 gaaaattgac cacttagtgc cacaccaaga gtagcaaacg cttctcgcgg gccaaaatag
     2221 ggtttgttga aaggaatatg cacctttgtt taatttgtgg tttaggctgc gaaattacat
     2281 attctcgtgc tcgggtcgaa atccttacct tcgcatatga atttatggca aaacatagcc
     2341 ttataagaga tattagcgga gtaatgggca gtaatattct ggccatggtc agtactctgg
     2401 gcataagcat tctgctttca cgaattcttg gcccccaagg atatggcctc tttacttcct
     2461 tatatgttgt gcctcaagtg gtaatgggtt tggtgcaatt gggaatgagc cgttcggctg
     2521 tattccatct aggccataaa aaatttaacg aatgccaaac catttcttcc atctatgtga
     2581 tgttgtgtat gacttctact gttgggatca tcgtatcggc tattgcttat gccatattga
     2641 ataatccatt gctcgaaacc aattggattt tactggcttt gtgcattatt ccattcgcac
     2701 tgg
//
LOCUS       DAORPD010000142         2682 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_6789_length_2682_cov_2.290445, whole genome shotgun sequence.
ACCESSION   DAORPD010000142 DAORPD010000000
VERSION     DAORPD010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6789_length_2682_cov_2.290445"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1988
                     /locus_tag="PK976_02410"
     CDS             <1..1988
                     /locus_tag="PK976_02410"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HRC66771.1"
                     /translation="PPKIKSVALNPVSPGEGQVVTVMAKVTDDAGVKSVNLYYKTTAD
                     FTKVAMQADTANFYKGQIPGQNKDITVSYYIEAENINGMKSYYPSSAPQMPASYKVGA
                     PAIFILINEIYTRGVPEDPDWVEIYNNSDVQMDISGYLIYDAGGQSGAKPKKAFPEGS
                     VIPAHGFLVIVVDDASESGFGLSSGGEEIWLENNKGAIIDDVTFPALEPTQSYGRFPD
                     GSPNWQILSTITKGTSNSPGIGPVANLKINEIYSTGDATTPDWVEIFNAGNQSVDLSG
                     WKIYDNGGQTGTKPKKEFPVGTSIPAGGFFVITVDDGSASGFGLSSNGEEIWLEKPDE
                     TIGDDVTFPALETGQSYGRYPDGSDNFQVFYVVTPGTANDNSLPPAAVVLKINEIYSR
                     GDAISPDWIEIYNAGTETVDLTGWKIYDNGGQAGTKPKKEFPSGATIDAGGFYVIIVD
                     DTATSGFGLSSSGETVWLEKPNGTVADEVVFPALEVGQSYGRYPDGSDNLQVLYVITP
                     GAPNDNSTPPASVILKINEIFSRGDDTNPDWIEIYNAATQDVDLTGWKIYDNGGQAGT
                     KPKKEFPAGEVIPANGFYVIITDDAAASAFGLSSNGETVWLENPEGTVVDEVTFPALD
                     VTQSYGRKPDGSDNFFIFTEITRGSSNNNASTLPKRK"
     gene            2016..2657
                     /locus_tag="PK976_02415"
     CDS             2016..2657
                     /locus_tag="PK976_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66772.1"
                     /translation="MAENLEIGKISKPRFEFRSFGQNFDNAAFRMSRLSVPVPENVWE
                     RHSDEIYIISRTNDVNNTKIRDGKMDIKTLVNISHGLEQWNPLMKGEFPMKTSSLLSE
                     VFMSFQLPLPNLNKEEYTFDEFLQIVYQHPDLGAVRVHKQRFGYMVNNTICEYAVVLI
                     NGARVVSVSSESTEIDDILKTISDLKLEGVENINYLQAIKRVIGMIHKPLANE"
BASE COUNT          820 a          555 c          606 g          701 t
ORIGIN      
        1 gccctcctaa aatcaaatcg gttgctctca atccagtttc acctggtgaa ggacaggtgg
       61 ttaccgtcat ggccaaagtt accgacgacg ctggagttaa atcggtcaat ctttattata
      121 aaaccactgc cgattttaca aaagttgcca tgcaggctga tacagcaaat ttctacaagg
      181 gccagatacc gggacaaaac aaggatatta cggtttccta ttatattgaa gctgaaaaca
      241 tcaatggcat gaagtcgtat tatccttcga gtgcacctca aatgccagct tcgtataaag
      301 taggtgcacc ggctatattt attcttatca acgagatata tactcgtgga gtacctgaag
      361 atcccgattg ggtggagatc tacaataatt ctgatgttca gatggatatt agtggatatt
      421 tgatttatga tgcaggcggt cagtcgggag ccaaacccaa gaaggctttt cccgaaggtt
      481 ctgtcattcc ggctcatggt tttttggtaa ttgttgtgga tgatgcatcc gagagtggtt
      541 ttggcctaag tagcggaggc gaagaaatat ggctcgaaaa caataaagga gccatcatcg
      601 acgacgtaac cttcccagct ttagaaccca ctcaaagcta tggacgtttc ccagatggca
      661 gccccaactg gcagatactc agtactatta ccaaaggaac ctcaaactca ccaggtattg
      721 gaccggtagc gaatctaaaa atcaatgaga tttattctac gggtgatgcc actactcccg
      781 attgggtcga aattttcaat gccggcaatc aatctgttga ccttagtgga tggaaaattt
      841 atgataatgg cggccaaaca ggtacgaaac ccaaaaagga gtttcctgta ggcacaagta
      901 ttcctgcggg aggatttttt gtaattacgg tggacgatgg ctctgccagt ggttttggac
      961 tgagcagcaa tggagaagaa atttggctcg aaaaacccga tgaaaccata ggcgatgatg
     1021 taactttccc ggctctcgaa accggacaat cttatggacg atatcccgat gggagcgata
     1081 acttccaggt attttatgtg gttactcccg gaacagccaa cgataattct cttcctccag
     1141 cagcagtagt attgaaaatt aatgaaatat attcacgtgg ggatgctata agccccgatt
     1201 ggattgaaat ttataatgct ggaactgaaa ccgtcgattt gactggctgg aaaatttacg
     1261 ataatggagg tcaggctggg accaaaccca agaaggaatt tccatcggga gcaactattg
     1321 atgctggtgg attttatgta attatagtgg atgatacggc taccagtggc tttggcttaa
     1381 gcagtagcgg agaaacggtg tggctcgaaa aaccaaatgg cactgtggcg gatgaagtag
     1441 tcttcccagc ccttgaagta ggtcaatcgt atggccgata tcctgatgga agcgataatc
     1501 ttcaggtttt gtacgtaata acacctgggg ccccaaatga taattccact cctccggctt
     1561 ctgtgattct gaaaatcaat gagatatttt cgagaggtga tgatacgaat cccgattgga
     1621 tcgaaattta caatgcagca actcaggatg tagatcttac gggttggaaa atatacgata
     1681 atggcggaca ggcgggaacg aaacccaaga aagaatttcc tgcgggcgaa gttatacctg
     1741 cgaatgggtt ttatgtaatt ataacagatg atgctgctgc aagtgctttt ggcctgagca
     1801 gcaatggcga aactgtatgg ctcgaaaatc ccgaaggaac ggtggtagac gaagttactt
     1861 ttccagcact cgatgtaact caatcgtatg gcagaaaacc tgatggcagc gataatttct
     1921 tcatctttac cgaaattact cgtggcagct cgaataacaa tgcatcaaca ttgccaaaac
     1981 gtaaataata aactaaccga ctaaaatgaa tttctatggc tgaaaacctc gaaatcggaa
     2041 aaatatccaa accaagattt gaattccgca gttttggtca aaacttcgat aatgctgcat
     2101 ttcgcatgtc gcgtctgtcg gtacccgttc ccgaaaatgt ttgggaacga cactccgacg
     2161 aaatctacat catttcccgc accaacgatg tgaacaatac caagatccgc gatggaaaaa
     2221 tggatatcaa gactctggta aatatttcgc atggactcga acaatggaat ccacttatga
     2281 aaggcgagtt tcctatgaaa acttcatcac ttctttcgga ggtcttcatg tcttttcagc
     2341 tccctttgcc taatcttaac aaagaagaat atactttcga cgaatttttg caaatcgttt
     2401 accaacatcc tgatttaggt gcagtacgcg tgcataaaca acgtttcggt tatatggtga
     2461 acaatacgat ttgcgaatat gccgttgtac tcatcaacgg ggcaagagtc gtaagcgtaa
     2521 gttccgaatc caccgaaata gacgatatct tgaaaaccat atccgatttg aaactcgaag
     2581 gtgtcgagaa catcaactac cttcaagcca tcaagcgcgt gataggaatg attcataaac
     2641 cccttgcaaa cgaataaatt atggcacaag aaatcgaaaa aa
//
LOCUS       DAORPD010000143         2681 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_6794_length_2681_cov_2.575781, whole genome shotgun sequence.
ACCESSION   DAORPD010000143 DAORPD010000000
VERSION     DAORPD010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6794_length_2681_cov_2.575781"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /gene="rpsB"
                     /locus_tag="PK976_02420"
     CDS             complement(<1..821)
                     /gene="rpsB"
                     /locus_tag="PK976_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HRC66773.1"
                     /translation="MSRVTFEELLEAGVHFGHLRRKWNPNMAPYIFMERNGIHIIDLY
                     KTIAKLEEATAAVKQMAKSGKKILFISTKKQAKEIVAEQAKRVNMPYVVERWPGGMLT
                     NFATIRKAVKKMAAIDKLMETENFLTMSKRERLQITRERAKLEKNLGSIADLTRLPSA
                     IFVVDILKEHIAVAEARKLNIPIIAMVDTNSNPNLVDFPIPANDDASKSIQLIVKTIA
                     DAIEEGLNERRFEKDQVEQEIEESYDETAEKKKLELDTSLEEEELVARNKLHKGTP"
     gene            complement(936..1322)
                     /gene="rpsI"
                     /locus_tag="PK976_02425"
     CDS             complement(936..1322)
                     /gene="rpsI"
                     /locus_tag="PK976_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HRC66774.1"
                     /translation="MDVINTSGRRKTAIARIYMSQGNGKIMINGRELNDYFKTNVLIY
                     KVLQPLELTNNQGKYDIRVNLEGGGITGQAEALRLAIAKALVEMNAEYRPILKDKGLL
                     TRDPRMVERKKPGRPKARKRFQFSKR"
     gene            complement(1326..1781)
                     /gene="rplM"
                     /locus_tag="PK976_02430"
     CDS             complement(1326..1781)
                     /gene="rplM"
                     /locus_tag="PK976_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008656690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HRC66775.1"
                     /translation="MNTLSYKTISANKQTVKKEWVLIDAENLVVGRLASEVAKILRGK
                     NKPYYTPHVDCGDHVVIINAEKIRFTGKKMDDKKYVSYSGYPGGQRSTTPKKLLKRVP
                     ERVIENAIRGMLPKNRLGRELFRNLHVYAGSSHPHQAQSPKQIKIIEAE"
     gene            2552..>2681
                     /locus_tag="PK976_02435"
     CDS             2552..>2681
                     /locus_tag="PK976_02435"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRC66776.1"
                     /translation="MSVKVDRVTKIYGSQRVLDDVSFQVEKGQITGLLGPNGAGKTT"
BASE COUNT          692 a          505 c          537 g          947 t
ORIGIN      
        1 ggagtcccct tgtgaagttt gtttctggct acaagttctt cttcctccaa ggaagtatcg
       61 agttctaatt ttttcttttc ggcagtctca tcatacgatt cttcaatttc ctgttcaacc
      121 tgatcttttt caaatcttct ttcattgagg ccttcttcta tagcgtcggc tatggtttta
      181 acgatcagtt gaatcgattt tgaagcatca tcattggctg gaatggggaa atctaccaga
      241 ttagggtttg aattggtatc taccatagct atgatgggaa tgtttaattt tctggcttca
      301 gctacagcga tatgttcttt gaggatatct acgacaaaga tggctgatgg gagacgggtt
      361 agatcggcga tggagccaag attcttttcg agttttgccc tttctcgggt aatttgaagg
      421 cgctcgcgtt tcgacatggt aaggaaattc tctgtttcca tcagcttgtc gatggcagcc
      481 atttttttaa ccgccttgcg aatggtggca aaattggtga gcatgccgcc aggccagcgt
      541 tcgactacat aaggcatatt gactcgtttt gcctgttcgg ctacgatttc tttagcttgt
      601 ttttttgtgg aaataaaaag tatctttttg cccgatttag ccatttgttt cactgcggcg
      661 gttgcttctt cgagttttgc aatggtttta tacaggtcga tgatatgaat cccattacgt
      721 tccataaaaa tgtagggagc catattagga ttccatttac gacgcagatg cccgaaatgc
      781 actccggctt ctaataattc ttcgaatgta actcttgaca tgaaatttag atatttaatg
      841 gtttactttc aactaaaatc aatccctaag tagtgctttg catagcaatc gcagggattt
      901 ggatactaaa cacaatttcc ccgtaacaaa taacattatc gttttgaaaa ctggaaacgc
      961 ttacgggctt ttggacgccc gggttttttg cgttccacca ttcgaggatc acgggtcaac
     1021 aatcctttat ctttaagaat tgggcgatac tcggcattca tttctaccaa tgcttttgca
     1081 atggcaagcc taagggcttc ggcttgtcct gttataccac ctccttccaa attaacccga
     1141 atatcatact tgccttgatt attggtgagt tcgagaggtt gtagtacttt gtaaatcaaa
     1201 acgttggttt tgaaataatc gttcagttct cgcccattta tcataatttt tccattgcct
     1261 tgcgacatgt aaattctggc aattgcggtc tttcttctac ctgatgtgtt gatgacgtcc
     1321 atactttatt cggcttctat gattttgatc tgtttgggtg attgagcctg gtgcggatga
     1381 gaactacctg cataaacatg caaattgcgg aataattctc tgccaagcct gtttttcgga
     1441 agcattcctc tgatggcatt ttcgataacc cgctcgggaa cgcgttttaa taattttttg
     1501 ggtgtagttg acctttgtcc accaggatag ccactatagc tcacatattt cttatcatcc
     1561 atttttttcc ccgtaaatct tatcttttcg gcattgatga taactacgtg atcaccacaa
     1621 tctacatgag gtgtataata aggtttgttc ttgcctctca ggattttggc aacttccgac
     1681 gccaatcggc ctacaaccag gttttcagca tctataagta cccattcctt tttaacagtt
     1741 tgtttgttgg cacttatagt cttataactt aaagtattca tcgatgagtt cattttctta
     1801 tactttgaaa agcttaaaaa tggggtgcaa aggtatggca atatttctta ttaaacaaac
     1861 ctatactctc aaattttttt ataaatattg ttgcaggtct ggtaatccgc ctattaattt
     1921 gttttgttaa tacactgtaa gtgatgtgca aagttataat aaatagaata aaatgtttag
     1981 cactctatca agtaacttcc tatagacaat gtggacggat aagcagtgta agggaattgg
     2041 cccctggcgt atttaatttc cttattattt atactaggaa catgccttat tcgttgtgag
     2101 gatgattttt gtcccttcag tttcacaaat tccacttata tgaaatcaag tcgattggga
     2161 agatccgtta tccattgatt tttaacaagt tgtatttcat tattgttcag aagcaaacaa
     2221 agttttgaat aataattgcc gagggcttat ctgtttttaa cgaataaagg atcaatcatt
     2281 tattcaagta ttcaagctgg gctattcgct tgagattact cgacgggtat ttttctgccg
     2341 taaaatgtat ggatttgaat tttggagccg aacctgaagg atgtattcca cacctcgtgg
     2401 aacagttatc cttaatatgg ttaaatgctg aaagaagttt cgcctttgtt ggtttttaca
     2461 tggcgataga cagtttgata aagatttgat cctattccat ctgaaacgtt tcacttaatt
     2521 ttgctttcgt attgttagaa attggcaaat tatgtcagta aaggtagatc gggttacaaa
     2581 gatttatggc tcgcaaagag tccttgatga tgtttctttt caggtagaaa agggtcagat
     2641 tactggcctg cttggtccta atggggccgg aaaaactacg t
//
LOCUS       DAORPD010000144         2677 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_6812_length_2677_cov_2.586575, whole genome shotgun sequence.
ACCESSION   DAORPD010000144 DAORPD010000000
VERSION     DAORPD010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6812_length_2677_cov_2.586575"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="PK976_02440"
     CDS             <1..435
                     /locus_tag="PK976_02440"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HRC66777.1"
                     /translation="NGSGDAELELKCKEFESYLNGSGDLLVEGVNGSCEIHVAGSGDM
                     KGKNFQLSNLKITNRGSGDIKLSGKADEVNIYSNSSGDIHCFELPCNKAEIHCYGSGD
                     VSLWVIQSITGSIHGSGDIRIKGNPNNRNIQKQGSGDIIWSN"
     gene            890..1651
                     /gene="tpiA"
                     /locus_tag="PK976_02445"
     CDS             890..1651
                     /gene="tpiA"
                     /locus_tag="PK976_02445"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HRC66778.1"
                     /translation="MRLKIVAGNWKMNLDFEEAEDLISAIADELENIDLGHAEVVICP
                     PFPYLEMALDIAIESVFSVGAQNISHFDNGAYTGEVSARMLASMDVDYCIVGHSERRK
                     YFNETDEIIAKKIDQAIEHEFITIFCCGELLSERQAGKHFDVVREQIEKAVFPLSEKE
                     FYNIVIAYEPVWAIGTGVNATPEQAQEMHAYIRNLIASRYGKDVADETSILYGGSCNS
                     KNAPEIFAQPDVDGGLIGGASLNADEFIEIVKAIL"
     gene            1658..2494
                     /gene="prmA"
                     /locus_tag="PK976_02450"
     CDS             1658..2494
                     /gene="prmA"
                     /locus_tag="PK976_02450"
                     /inference="COORDINATES: protein motif:HMM:NF001785.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006479 - protein methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HRC66779.1"
                     /translation="MEYTELRIPIIIDDETKELLPSILTELGFEGFIEEEDSLLGYIP
                     SVHFDRHAVEELLQRLDIAPFFTFRTVPEQNWNAVWESQFEPVVIAEKCFVRAPFHFP
                     QPHYPIELIIEPKMSFGTIHHETTAMMMEYLLEIDVMGKSILDVGTGTGILAILSAKI
                     GAVNIVAIDNNEWAYRNALENIQLNNTPRIVVKQGDASLLSTMNEFDVILANINRNIL
                     LNDIPAYMKVLKQNGLLIVSGFYKDDLPVIDDAFHRVGLASCRIKERNSWLAAVYLKT
                     LP"
BASE COUNT          789 a          509 c          565 g          814 t
ORIGIN      
        1 aatggaagtg gagatgccga gctcgagctc aaatgcaaag aatttgaatc ttatttgaat
       61 ggttcgggcg atttattggt ggaaggggtg aatggctcgt gcgagatcca tgtagcaggt
      121 tcgggcgata tgaaaggaaa gaattttcaa ctttcgaacc tgaaaattac caatcggggg
      181 tcgggcgata taaaacttag cggtaaagcc gatgaagtaa atatttattc taattcatcg
      241 ggtgatatcc actgttttga actgccttgc aataaagcag aaatacactg ttatggctca
      301 ggtgatgtga gcctttgggt gatacaatcc attactggct caatccatgg ctcgggagat
      361 atcagaataa aagggaatcc caataatcgt aacattcaaa aacagggctc gggcgatatt
      421 atctggagta actgaaggaa ttacttctca tgttcctttt gcctttcaat gcctttacta
      481 taactatttt tttcgaattc ccctattcat cctgtaatcg aaggtaattg tcctgtggaa
      541 aagttacagg atggaaatat tccatttttg ttatgaaata aagaggatga tttgatggac
      601 tttgaaactg aagttacgaa ttcggacagg ctcattgttc aggttccata tcacaattct
      661 ggaatgatga tttacttgga aacataaaat gacaatagat tttccaaatt aactatccga
      721 gcgaatggcg cgtttcttat tttatggtag attcgatcgg ctattcgttt accaatagat
      781 tttagaattt tactttgctc ccggcagtga acagttatat ttattatttt cgcacgtttg
      841 attttgtttt aattcattct gaatatataa tttaaaaaat tgttttatca tgagactaaa
      901 aattgtagcc ggtaactgga aaatgaattt agacttcgag gaagccgaag atcttatttc
      961 cgccatagcc gatgagcttg aaaatatcga ccttgggcat gccgaggttg taatttgtcc
     1021 cccattccct tatctcgaga tggccctcga tatagccata gaatctgttt tttcggtagg
     1081 agctcaaaac atcagtcatt tcgacaatgg agcctatact ggggaagttt cggcccgtat
     1141 gctggcttcg atggatgttg attattgtat tgttggccat tcggaacgaa gaaaatattt
     1201 taacgaaacc gacgaaatca ttgcaaaaaa aatcgatcag gccatcgaac atgagtttat
     1261 cactatattc tgctgtggcg aacttttaag tgaacgtcag gccgggaaac atttcgatgt
     1321 agttcgtgaa caaatcgaga aagccgtctt tcctttgtcc gaaaaagagt tttacaacat
     1381 cgtcattgcc tatgaacccg tctgggctat tggcactgga gtgaatgcca cacctgaaca
     1441 ggctcaggaa atgcatgctt acattcgcaa cctcatcgca tcgaggtatg gtaaagatgt
     1501 agctgacgaa acctccatat tgtatggtgg aagttgcaat tcgaaaaatg ctccggaaat
     1561 tttcgcccaa cccgatgtgg atggcggttt gattggcgga gcaagcctta atgccgatga
     1621 atttatcgag attgtcaagg caatcctttg attaaaaatg gaatatactg agctgcgaat
     1681 ccctataatt attgacgacg aaaccaaaga attactccct tccattttaa ctgagctggg
     1741 atttgaaggt tttatcgaag aagaagattc tttgctggga tatatccctt cagttcattt
     1801 tgaccgccat gccgtagaag aattattgca gagactggat attgctcctt tctttacctt
     1861 tcggactgtt cccgaacaaa actggaatgc tgtttgggaa agccaattcg aaccggtcgt
     1921 tatcgctgag aaatgctttg taagagctcc ttttcacttt cctcagcctc attatcccat
     1981 cgaactgatc atcgaaccca aaatgtcgtt tggtacaatc catcatgaaa ccaccgccat
     2041 gatgatggaa tacctgctcg aaatcgatgt gatggggaaa agcattctgg atgtgggaac
     2101 cggtactgga atcctggcca ttctttcggc aaagatagga gccgtcaata tcgttgctat
     2161 tgataacaat gaatgggcct accgaaatgc tctcgaaaat attcaactca acaatacacc
     2221 tcgaatcgtc gtaaaacaag gagatgcatc tttgctttcg actatgaatg aatttgacgt
     2281 cattttggcc aatattaacc gtaatattct tttgaatgat attcccgctt acatgaaggt
     2341 tttgaaacag aacggtctat tgatcgtaag cggtttttac aaagatgatc taccagttat
     2401 agatgatgct tttcaccgag taggcctcgc atcttgccga ataaaagaaa gaaacagttg
     2461 gctggctgct gtttatttga aaactctgcc ttaatgattc gaaacttatc tttcgtatcg
     2521 aaaaacctat aaaaaaagaa gtccttcacc aattttatcc tcgaatctct catccttatg
     2581 gatcatatta aggcaaatga ttatttcttc ccatgaatcc gaagatggat cattgtttat
     2641 tggctatcag cttacagctt ttagagaaga tggcaag
//
LOCUS       DAORPD010000145         2673 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_6825_length_2673_cov_2.380825, whole genome shotgun sequence.
ACCESSION   DAORPD010000145 DAORPD010000000
VERSION     DAORPD010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6825_length_2673_cov_2.380825"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="PK976_02455"
     CDS             complement(<1..872)
                     /locus_tag="PK976_02455"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HRC66780.1"
                     /translation="MSNSWIDRVRERINIFLYPHKETLLWVLRILSIIVSLVAVCAIV
                     YYHGFPRTPHTDDIVRIITIFSLAFYALKYFLNFFYDFHPLAYLRRTWFEGILVLFII
                     LMGLFYLIVGQQRIYGFFAQRGMEQFPDLSILFIQFYVFIMVLLETAKAGQYIDRIKI
                     GPAGLLALSFLILIAFGTAMLLLPEMTVDHHIQPVDALFISTSASCVTGLSTVDISNY
                     FTFKGQVVILILIQLGGINIISFATFFALFYTGAEGLKQQSLLKELLDTSGLSDARRI
                     LKRIILYSFTIEIAG"
     gene            complement(900..1595)
                     /locus_tag="PK976_02460"
     CDS             complement(900..1595)
                     /locus_tag="PK976_02460"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HRC66781.1"
                     /translation="MVHRKIAVIGIGRFGESIAKNLSKKGAEVLAIDKLQENIERVSD
                     DVSLAVAIDATDKKALLSQNIQEFDAVVVSIGESFEQRLLCVALLLDLGVKRIIARFG
                     GKSQRIILEKLGVKDLWSPEEEVGAIVAERLLNPSVVSYLQLPDDYRIVEMLTPKKVA
                     GRMLGDLDLRDRYKLSLITIKEETTTKVNNMEMSIYHVAGVPDSKYVIKLTDRLVLFG
                     KNKDIDRFIQINE"
     gene            2103..>2673
                     /locus_tag="PK976_02465"
     CDS             2103..>2673
                     /locus_tag="PK976_02465"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HRC66782.1"
                     /translation="MKPFIGIVFCVFSILPSRIFAKDQNWTTYCESTDFRRTPRYKET
                     IHFCKQLAENSHIIAYEEFGISPQGRALPLLIMDKDRNFSPELARRAGKAILMIQACI
                     HPGEPEGKDAGLILFRNLGILGMDTSLLRHVTLVFIPIFNVDGHERFSPYHRINQNGP
                     EEMGWRTTAQNLNLNRDFMKADAPEMQAWL"
BASE COUNT          837 a          547 c          509 g          780 t
ORIGIN      
        1 cctgcaattt caatggtaaa gctatataga ataattcgtt tcaagatccg gcgagcatcc
       61 gaaagtccgc tggtatccaa aagttctttc aataatgatt gctgtttcag cccttcggca
      121 cccgtataaa ataatgcaaa aaaagtagca aacgaaatga tattgattcc tcctaattga
      181 ataaggatta agatcaccac ctgtccctta aaggtaaagt aattggaaat atccacagtg
      241 gataagcctg taacacaaga agcgctggtg ctgataaata aagcatccac gggttgaatg
      301 tgatgatcga cggtcatttc gggtaacaat aacatagccg taccgaaagc aatcaatata
      361 agaaaagaca gggcaagtaa gcctgcaggg ccaattttga tcctatcgat atattgacct
      421 gctttggccg tttcgagcag caccatgata aaaacataaa actgaatgaa aagaattgaa
      481 agatcgggga attgttccat tcctctttgg gcaaaaaagc catatattct ttgttgaccc
      541 acaatgagat agaaaaggcc catgaggatg ataaataaaa ccagtattcc ttcaaaccag
      601 gttcttctca aataggccaa aggatggaaa tcatagaaaa aattcaagaa atatttcaaa
      661 gcataaaaag ccaaactaaa gatggtaata attctgacaa tatcatcggt gtgaggagta
      721 cggggaaatc cgtgataata aactatggca caaactgcaa caagtgaaac tataatgctg
      781 agaattctca agacccatag cagggtttcc ttatgaggat aaagaaagat atttattctt
      841 tcccgcacac gatctatcca ggaattagac ataattctca gtaaaactta atggtttaat
      901 tattcgttga tctgaataaa acggtcgatg tctttatttt taccaaataa tacaagcctg
      961 tcggtgagtt taattacata tttcgaatcg ggcactcctg ccacatgata tatggacatc
     1021 tccatattgt tcacttttgt ggtggtttct tccttaatgg ttatcaagct tagtttataa
     1081 cggtcgcgaa gatcgaggtc accaagcatt cgcccggcaa cttttttagg tgtcagcatt
     1141 tctacgattc gataatcgtc gggaagctgc aaatacgaca ctacggatgg attgagcaga
     1201 cgctcggcta caatggctcc tacttcttcc tcgggtgacc acaagtcctt tacacctaat
     1261 ttttcgagta ttattctttg gcttttccct ccaaaacgtg caataatacg tttcacgccc
     1321 aagtcgagta ataacgccac acaaagcaat ctttgctcga aactctcccc tatggaaact
     1381 actactgcat caaattcctg tatattctga ctcaaaagtg cctttttatc tgttgcatca
     1441 atagccactg ccagagaaac atcatccgaa acacgttcga tgttttcctg aagcttatca
     1501 atagccagta cctctgcacc cttcttcgac agattcttcg caatggattc cccgaaccgg
     1561 cctatgccta taacggctat tttcctatgt accatataaa tgaatttaga tggcttgcaa
     1621 tttggaggcg caaatatatt tcaattttat gcattttaca cattccagac caaactcgaa
     1681 aaatattcat aaaaaatttc ggtgtttttg tgatatggct tacagcttta aacaaaagtt
     1741 ttaaaaaatg gcattaagcc tgctggcatg gtaaacttaa atcatgcctt taaatggaaa
     1801 ggagcagatc ggtttttcca gaaatttatt gagctcgagc aaagccaaaa cccatcctta
     1861 cattaaagca gatgatgatt acgataaatc gaaaggtgaa atcttcaact tcattctgca
     1921 ttttatatat cgatattacg atcgttagag atattttagc cacctctttt cgttgtaata
     1981 aatttactgc cacagcatat catcgagtac ataacaggca aagcttgagg taaacgaggc
     2041 cgaacataat tgaatattgc gcacttgtca taattttgcg ccggatgaat ctttttgaag
     2101 aaatgaaacc ttttatcgga atagtttttt gtgtcttttc cattcttcct tccagaattt
     2161 tcgctaagga tcagaattgg actacttact gcgaatcgac tgattttagg agaacgccgc
     2221 gatataaaga aaccattcat ttttgtaaac agcttgccga aaattcccat atcattgctt
     2281 atgaagaatt tggaattagt cctcagggcc gagcactccc tcttctcatt atggataagg
     2341 atagaaattt ttcgcctgaa ctagcccgtc gggctggaaa ggccattttg atgattcagg
     2401 catgtattca tcctggagaa cccgaaggga aagatgctgg attgattctg tttcgcaact
     2461 taggtatttt agggatggat acttctttac tccggcatgt taccttggtt ttcatcccga
     2521 ttttcaatgt agacgggcat gagaggttta gcccttatca tcgtatcaat caaaatgggc
     2581 ccgaagagat gggctggcgt accacggctc aaaatctcaa tctcaaccga gattttatga
     2641 aggctgatgc ccccgaaatg caggcttggc tgc
//
LOCUS       DAORPD010000146         2661 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_6866_length_2661_cov_2.471220, whole genome shotgun sequence.
ACCESSION   DAORPD010000146 DAORPD010000000
VERSION     DAORPD010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6866_length_2661_cov_2.471220"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1114
                     /gene="lepA"
                     /locus_tag="PK976_02470"
     CDS             <1..1114
                     /gene="lepA"
                     /locus_tag="PK976_02470"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HRC66783.1"
                     /translation="ATGKEYHADEIGILGLERKPCNELSAGNVGYIISGIKSAKEVKV
                     GDTITHVERPCDKPIEGFSNVKPMVFAGIYPVDADEYEELRGSLEKLQLNDASLVFEP
                     ESSAALGFGFRCGFLGLLHMEIVQERLDREFDMDVITTVPNVSYKVYTTKGEVIDVYN
                     PSGLPDPTQIHHIEEPYIRANIITKAEFVGAIMKLCIEKRGTLVNQTYLTTDRIELIV
                     DLPLGEIVFDFYDKLKSISKGYASFDYHIIGYKEANLVKLDILLNGDSVDALSTLIHR
                     DNAYNFGRRICEKLKELIPRQQFDIAIQAAIGSKIIARETVKALRKDVTAKCYGGDIT
                     RKRKLLEKQKKGKKRMRQIGNVEVPQSAFLAVLKLD"
     gene            1196..1678
                     /locus_tag="PK976_02475"
     CDS             1196..1678
                     /locus_tag="PK976_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRC66784.1"
                     /translation="MTTAEYNHCVEQHADNVYRFVVKNIGNEQVAKDIVQEVFTKMWE
                     KASTIAYQKSKSYLFTAAYHTLIDTVRYQNRFASLETSPEQAYEPSNYDGIKGILEEA
                     LNRLPPVQKAVVMLRDYEGYSYAEIGEITGLSESQVKVYIFRARQSLKNYLVTIDNLI
                     "
     gene            1691..>2661
                     /locus_tag="PK976_02480"
     CDS             1691..>2661
                     /locus_tag="PK976_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66785.1"
                     /translation="MNTINTDNCEAYFLDYYEGRLSKDEIASLRQFLAMHPEWQTKFE
                     DWENIHLPNIQLSFPEKEWLKHPSADEAINISFSNYEYYFIAALEGELTQPQRYSLDL
                     FLKQHPELEKEFDLYALTRLKPQHIPFPDKKQLLHKKKNIPLKYFVPALAAAAAIMLL
                     ILFQPSKHRNYPPASDRPFAQTSTVQEHSLTPLSLPSIIPSTEEKTTRKIQPAAKLTS
                     TSLPQRQVLIPIANMPSKEITNISIHTTPPEVKNIHRIQTAIYMEMIAQQQNNTNEDR
                     PHLLAKIVKPVVGLFQKKSNDEMANRNEEINLWTLAEYTIKGFNAIT"
BASE COUNT          863 a          612 c          502 g          684 t
ORIGIN      
        1 tgccacgggt aaagaatatc atgccgatga aataggaata ttgggtttgg agcgtaaacc
       61 atgcaacgag cttagtgctg gaaatgttgg ttatatcatt tcaggaataa aatcagcaaa
      121 ggaagtcaag gtaggcgata ctatcacgca tgtggaacga ccttgtgata aacccatcga
      181 aggattcagc aacgtgaaac ccatggtttt tgctggtatt tatcctgtag atgccgatga
      241 atacgaagaa ttacgcggtt cgctcgaaaa gctacaactc aacgatgctt cactggtgtt
      301 cgaacccgaa tcatcggccg cgctgggttt tggatttaga tgtggatttc tgggattgtt
      361 gcacatggaa atcgttcagg aaaggctcga tagagaattc gacatggatg taattactac
      421 cgttcctaac gtttcctata aagtttacac caccaagggc gaagtaatag atgtttacaa
      481 tccaagcggt ttacccgatc ccacacaaat tcaccatatc gaagagcctt acatccgcgc
      541 caatatcatt acgaaagcgg aatttgttgg agctatcatg aaactctgca tcgaaaaacg
      601 cggaacactg gtcaatcaaa cttatcttac aaccgaccgc atcgagctta tcgtcgatct
      661 tcccttggga gaaattgtat tcgattttta tgacaagttg aaatccattt cgaaaggtta
      721 tgcctcattc gactatcaca tcataggata caaggaagcc aatcttgtaa aactcgacat
      781 attgctcaac ggcgattcgg tagatgcact ctccacactg attcaccgtg acaatgccta
      841 taattttggc cgccgtatct gcgaaaaact caaagaactc attccccgcc aacaattcga
      901 tatcgccatt caggcagcca taggttcgaa aatcattgcc cgcgaaaccg taaaagcctt
      961 gcgcaaggat gtcaccgcca aatgttatgg aggcgatatt acacgtaaaa gaaaactcct
     1021 cgaaaaacaa aagaaaggga aaaaacgtat gcgccagatt ggaaacgtcg aagttcctca
     1081 atcggctttt ctggctgttt taaaactgga ctgataagca agaattctac attttttgta
     1141 acttttccag cattcctccg tctttagtta atcattcgaa catcccaaaa ttctcatgac
     1201 cactgccgaa tataatcatt gcgtggaaca acatgccgat aatgtttacc ggtttgtagt
     1261 caaaaatatt ggaaatgaac aagtggccaa ggatatcgtt caggaagtgt ttacgaaaat
     1321 gtgggaaaag gcctcgacta ttgcttatca aaaatcaaaa tcctatctgt ttacagcggc
     1381 ttatcataca ttgatcgaca cggtgagata tcagaatcgt tttgcaagct tggaaaccag
     1441 ccccgagcaa gcttacgaac cttctaatta cgacggcatc aaaggaatat tggaagaagc
     1501 cttaaaccga ttacctccgg tacaaaaagc cgtcgtgatg ttgcgagatt acgaagggta
     1561 ttcctatgcc gaaattggtg aaattacggg actgagcgag agccaggtga aggtttatat
     1621 ctttcgcgct cgccagagcc ttaaaaatta tcttgtgact atcgataacc ttatttaaag
     1681 actcgaagtg atgaatacca ttaatacaga caattgcgaa gcctatttcc tcgattacta
     1741 cgaaggcaga ttaagcaaag acgaaattgc ttccttaagg caatttttgg ctatgcatcc
     1801 agaatggcaa accaaattcg aggattggga aaatattcac ctgcccaata tccaattgtc
     1861 attccccgaa aaggaatggc tcaaacatcc ttcggcagac gaagccataa acatcagctt
     1921 cagcaactac gaatattatt tcattgctgc cttagaaggt gagcttaccc aaccccaacg
     1981 ctacagcctc gatctcttct tgaaacaaca tcccgaactg gagaaggaat tcgatctgta
     2041 tgctttaacc cgcctgaaac cacaacatat tcccttcccc gataaaaaac aattgttaca
     2101 taaaaagaaa aacatacctc taaaatactt cgttccagcc ttggctgcag cagccgctat
     2161 aatgttattg atattatttc aaccttcgaa acatcgaaat tatccaccag catctgatag
     2221 accatttgcc caaacttcaa cagttcagga acattctctt actccgcttt ctttgccctc
     2281 tattatcccc tcaacagaag aaaaaaccac ccgcaaaatc caacctgctg caaagctcac
     2341 ttctacttcc ttgcctcagc ggcaagttct cattcctata gccaacatgc catcaaaaga
     2401 aattacaaat atttctatac atacaactcc tcccgaagtt aaaaatatcc atcgcataca
     2461 aacagccatt tatatggaaa tgatcgcaca acagcaaaac aatacaaatg aagaccggcc
     2521 tcatttattg gccaaaattg tgaaacccgt tgttggacta ttccagaaaa aatcgaacga
     2581 tgaaatggca aacagaaacg aagagattaa tttatggact ctggccgagt ataccatcaa
     2641 ggggttcaat gccataactc a
//
LOCUS       DAORPD010000147         2653 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_6905_length_2653_cov_2.278291, whole genome shotgun sequence.
ACCESSION   DAORPD010000147 DAORPD010000000
VERSION     DAORPD010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6905_length_2653_cov_2.278291"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(197..589)
                     /locus_tag="PK976_02485"
     CDS             complement(197..589)
                     /locus_tag="PK976_02485"
                     /inference="COORDINATES: protein motif:HMM:NF022769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3127 domain-containing protein"
                     /protein_id="HRC66786.1"
                     /translation="MALTLKGKVVKILPIQQGEGRNGVWKKQSFIIETFGYYPRKICL
                     IAWNERTDVVSSLEIDEEVSVECSIESREFNDRWYTDVRALNISKSTPSSISSSSDSE
                     EIHSQPESPSEPPAIEYNEIQDDNDLPF"
     gene            complement(611..1078)
                     /locus_tag="PK976_02490"
     CDS             complement(611..1078)
                     /locus_tag="PK976_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008822717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HRC66787.1"
                     /translation="MEEQLIQEVIDLAFQNVMEGKGGPFAAMVVRGGHIIAIATNTVT
                     STNDPTAHAEVNAIRQACKQLNTFQLDDCELYTSCEPCPMCFGAIYWARLRKVYYAAR
                     QEDAAQAGFDDSFIFEQLTLPSSERKIPFVCMHHHEAIKPFVLWNRMEEKIKY"
     gene            1511..>2653
                     /locus_tag="PK976_02495"
     CDS             1511..>2653
                     /locus_tag="PK976_02495"
                     /inference="COORDINATES: protein motif:HMM:NF024882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HRC66788.1"
                     /translation="MAAKVNHKTEKEGIEEKGKPSNKPHKKKQKSTAWWRQKEKSRAV
                     IGFIITAIGIYLLLAFISFFINWHIDFDKTTNISFYDIASGKTNFKNTTGSIGAAISY
                     YIIYKGFGLASFVFPFIFILIGLRIALGIRIFPLHKSFSISFFILIWLSVTLGFAFHS
                     TNNLSNILGGVFGNQISRYMMVVMGKTGLVILLLALLLVFTTIYMNFPFSQIQNYRRR
                     KQEEKKNKLQPSPSTVPAPSVSYNLHEFAVDEPATTESKIIDKPSPKKAENNSPSNPQ
                     PNSKSAREEVEEGTPGAQPKNVDKPVSSHDEVELIIVNSKNKEQTNKEPEITEEKGNP
                     ITHYTLDTLYDPTLELSDFKLPTADLLQTYPNENANVYKEEIEDNKK"
BASE COUNT          860 a          529 c          476 g          788 t
ORIGIN      
        1 cttcataaac cgtaagtcct tgtgaaaaga tattttcatc aatttgagtg gcttcggtga
       61 ttttatcatc catccaaata tattttctca acggaataat cattttcgaa aaattagatg
      121 ttatgtttag attcttaatc ctgtactccc gagaaattca atgatcatat cattagaaaa
      181 gcattggggt atatctttaa aatggtaaat cattgtcatc ttgaatctca ttatattcta
      241 tagccggagg ttcagaggga gattcaggct gtgaatgtat ttcttccgag tctgatgaag
      301 atgaaataga agaaggggta cttttcgaaa tattcaaggc tcttacatcg gtgtaccaac
      361 gatcgttgaa ttcacgagac tctatcgagc attccacgga aacttcttcg tcgatctcaa
      421 ggctcgaaac tacatctgtg cgttcattcc atgctatcaa gcatattttt ctgggataat
      481 aaccgaaggt ttcgatgatg aaactctgtt tcttccatac cccatttcta ccttcgcctt
      541 gttgaatcgg caaaattttt acgacttttc ccttaagtgt tagcgccata gctgaatata
      601 tttttaaatt tcaatatttg atcttttctt ccattcgatt ccatagtaca aagggtttta
      661 tggcttcgtg atgatgcatg caaacgaaag gtatttttct ttccgaagat ggcaaagtta
      721 gctgttcaaa aataaacgaa tcatcgaaac ctgcctgagc tgcatcttcc tgccgggcag
      781 cataataaac tttcctaagc cgagcccagt aaatagctcc aaaacacatc ggacaaggct
      841 cacaactggt atacagctcg caatcgtcga gctggaaggt atttagttgc ttgcaggctt
      901 gccttatggc attcacctcg gcatgagccg ttggatcatt cgtggaagtc actgtattgg
      961 tggcaatggc tattatgtgg ccgcctcgta caaccatagc cgcgaatgga cctcctttcc
     1021 cttccattac attctgaaag gccaaatcaa taacttcctg tatcaattgt tcttccatct
     1081 aatcatgtat tcattcgttt cttctttcaa aaatacacga gaattttcga acatcattca
     1141 aaaaaacaga ttcatttaac aaaatattct gcagggatga gaaatcgatg aaaacgttta
     1201 ccatcgcatc tccaacaaaa atattccagt tggcaaatgg ttgaaaatca aataaagtca
     1261 ttattcatga aattactcga agtcttatat ttcaggtttt tatcaatctt cgtgaagata
     1321 atgccatggc taacgtgcca aaaatatcga tctaatcata agccgaaaag ttttttcgca
     1381 gatattcgaa gagaagaaag gcttcgaacg agataacacg accttagcaa agaaagaaca
     1441 tttcaaatgc cgaaaagacg aaagttaaaa ttgattattt ttgcaataat caacctgatt
     1501 cggagatagg atggcagcga aagtgaatca taaaacggag aaagaaggga ttgaagagaa
     1561 gggaaaacct tctaacaaac cgcataagaa aaaacaaaaa tctacggcat ggtggcgcca
     1621 aaaagaaaaa tcaagagcag ttatcggttt tataataaca gcaataggca tttatttgct
     1681 tctagctttt atttcatttt tcatcaactg gcacatcgat ttcgataaga ctaccaatat
     1741 ttcattttat gacattgcat cggggaaaac caatttcaaa aacacgacag gttcgatagg
     1801 tgcggcaatt tcctattaca ttatttacaa aggatttggt ttggccagtt ttgttttccc
     1861 atttattttt attctgattg gattacgtat tgctttaggt attagaattt tccctctcca
     1921 caaatccttt agtatttcat ttttcatttt aatctggtta tcggtaactc tgggttttgc
     1981 ctttcatagt accaacaacc tgtctaatat tttaggaggg gtattcggca atcaaataag
     2041 tcggtatatg atggttgtaa tgggaaaaac cggattggtc atacttttac tggcgctgtt
     2101 actggtattt actaccattt acatgaattt ccctttttcg cagattcaaa actacaggcg
     2161 tcgcaagcag gaagaaaaga aaaataaatt gcaaccctct ccttcgaccg ttccagcacc
     2221 ttctgtaagt tataaccttc atgagtttgc ggtggatgag cctgcaacta cagaatcaaa
     2281 gataatcgat aaacccagcc caaaaaaagc agaaaacaat tctccatcca acccgcagcc
     2341 aaattccaaa tctgctcggg aagaagttga agaaggaaca cctggcgctc aacctaaaaa
     2401 cgtggataag ccagtaagca gccatgatga agtcgaattg attatagtga attccaagaa
     2461 taaggagcaa accaacaaag aaccagaaat aactgaagaa aagggaaacc ccattactca
     2521 ttacactctc gatacgcttt atgatccaac cttagaactt tccgatttta aacttcctac
     2581 ggccgatctt ttgcaaacat atcccaacga aaatgccaat gtttacaagg aagaaatcga
     2641 agacaacaag aaa
//
LOCUS       DAORPD010000148         2651 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_6909_length_2651_cov_2.442989, whole genome shotgun sequence.
ACCESSION   DAORPD010000148 DAORPD010000000
VERSION     DAORPD010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6909_length_2651_cov_2.442989"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="PK976_02500"
     CDS             complement(<1..314)
                     /locus_tag="PK976_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HRC66789.1"
                     /translation="MIKGKEARLHIGIFGRRNNGKSTFINCLSGQELSIVSDIAGTTT
                     DPVTKAMEIGGLGPVVFIDTAGIDDSGPLGQKRVQKTLEILKTIDLAILLIAENAFGE
                     QE"
     gene            complement(326..1762)
                     /locus_tag="PK976_02505"
     CDS             complement(326..1762)
                     /locus_tag="PK976_02505"
                     /inference="COORDINATES: protein motif:HMM:NF012432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HRC66790.1"
                     /translation="MRTEKDFMGELQLPDDALYGIQSLRARKNFPHHSHFFEEWYRAM
                     GIVKHACYLTYLSFCRAVQQKNLESRLPSPLISSEIMDGLVQASIEVSRGEHFEHFIV
                     PGISGGAGTSINMNINEIIANRALIISGHSIGDYHYIDPILHANIYQSTNDTVPTALK
                     IAMLNLLGQLEQEINLLRAETERLETASRNILRVGRTQMQDASPSSYGILFSTYSEAF
                     SRDWWRMSKCFERIKSVNLGGSVIGTGIGVPRFFIVEVITHLQTLTGLPLSRSENMPD
                     ATANLDSLAEVHGIIKAHAINLEKIANDIRLLSSDIASPGEISIPQKQIGSTIMPGKA
                     NPVIPEYVISCVHHVYANDEVITSLVASGCLDSNAYLPIIGHCLIDSLKTLISANKTL
                     KDNLLNDLKFHPTVAAHKTWNSPAITVALSPFIGYYKASQLACEMENTHCDVFTANQR
                     LHLINPDKLKEIMRTENLLKLGYSLNEL"
     gene            2465..>2651
                     /locus_tag="PK976_02510"
     CDS             2465..>2651
                     /locus_tag="PK976_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66791.1"
                     /translation="MKNKSLLFFLTALMMGCGSYLFASGIAGSKGNEGDRPGKAQLQN
                     HLKSGNAVSLNWQSLGPD"
BASE COUNT          819 a          526 c          531 g          775 t
ORIGIN      
        1 tcttcctgtt caccgaaggc attttcggca ataagaagta tagccaggtc aatggttttg
       61 agtatttcaa gggttttttg cacgcgtttc tgccctaagg gtccgctgtc gtcgatgccg
      121 gctgtatcga taaataccac gggacctaat cctccaatct ccatggcttt ggttacgggg
      181 tcggtggttg taccggctat atcgctcact atggataatt cctgccccga caaacaattg
      241 atgaatgtac ttttgccatt gtttcttcgc ccaaaaatgc caatgtgaag tctggcttct
      301 tttcctttga tcatgatatt aagaattata attcgttgag ggagtatcca agtttcaaga
      361 ggttttctgt acgcataatt tcctttagtt tatcggggtt tatcagatga agccgctggt
      421 tagccgtaaa aacatcacaa tgtgtgttct ccatttcgca agccaattgt gaagccttgt
      481 aatatccaat gaacggactc aaggcgacag tgatcgccgg gctgttccaa gttttatgag
      541 cagcaactgt gggatgaaat ttcagatcgt tcaacaaatt atccttcaat gttttattag
      601 ccgagatcaa ggtcttgagg ctatctatca agcaatgacc aatgatagga aggtaagcat
      661 tcgagtcgag gcagcccgaa gcaaccagag aagtaattac ctcatcgttg gcataaacat
      721 gatgaacgca gctaatcaca tattcgggaa tcacagggtt tgctttacct ggcataattg
      781 tgcttccgat ttgcttctga ggaatgctga tctcgcccgg acttgctata tccgacgaaa
      841 gcagccgtat atcatttgca attttttcga gattaattgc atgagccttt ataattccat
      901 gaacttcggc aaggctatcc agattggcag ttgcatcggg catattctcc gagcggctca
      961 aaggtaaacc cgtcaaagtc tgaagatgag tgatgacttc gacgataaaa aaccttggca
     1021 caccaatgcc agtaccaata acgctgccac ccagatttac cgattttatg cgttcgaaac
     1081 atttgctcat acgccaccaa tcgcgtgaaa acgcctcact atatgtagaa aataaaattc
     1141 catacgaaga aggcgaggca tcctgcattt gtgtgcgtcc tactcttaga atatttcgcg
     1201 aagcagtttc caatctttcc gtttcggctc gcaataaatt aatctcttgc tcaagctgtc
     1261 caagcaaatt cagcatggct atcttcaagg cagtcggtac ggtatcgttg gtcgactgat
     1321 aaatattggc atggagtata ggatcgatat agtggtaatc cccaattgaa tggcccgaaa
     1381 tgataagcgc tctgttggcg atgatttcat tgatattcat gttgatgctc gttccagcgc
     1441 ctccacttat tcccggaacg ataaaatgtt cgaaatgctc acctcgactc acctcaatag
     1501 aagcctgaac gagcccatcc attatttcag aagatataag aggagaaggc aaacgtgatt
     1561 ctaaattctt ctgctgtacc gctctgcaaa acgataaata agtgagataa catgcgtgtt
     1621 tcactatgcc catcgcccga taccattctt caaaaaaatg agaatgatgc ggaaaattct
     1681 tacgagctct caacgattgt atcccataaa gagcatcgtc cggcaactgg agttccccca
     1741 taaaatcctt ctctgttctc ataaatttca aattgagttc ataaaattaa attcggagat
     1801 tttgtcgaca ttgcataatc ccgaacgatg gataaatccc cttcatgaat tcttcaacct
     1861 taacaaactt cgaaaacttt catcttttat cgaattgaaa ttcattattg ttagaataaa
     1921 tgaattcgct tatggccaaa ggcattggcg gaaacccaaa agattaaatc cgaagatgca
     1981 aagttaactg aaaagctgca atctattttg cgggcaggca atcgaagaag aaagaaaaat
     2041 ttctgattct gtcagcaaac tcaaagaagc gtatggatat agaaacatct aaaaaaatgg
     2101 acctgcttta aaacaagtat ctcaggaatt attacttttg tattccaaaa caaagaatgg
     2161 attttatatt catacatttg ataatttgtc aattacaaaa atgaagtatg attcgacaaa
     2221 tgaatgatga aaagaaaata aatttttatt tcatgttgaa tgttcgaaag taaaaattga
     2281 tttaattttg taagatgaaa taattaaatg attgataatc tttatgaagg atcaccgaaa
     2341 aagttttgag cataatattg tggaaatttt tttacataga aatacttgca gattcataaa
     2401 gagtatgtaa cttgtgccac tattttaaaa ggataagaac tatatttaaa caaatcttaa
     2461 tgatatgaaa aataaatctt tactgttttt cctgacggcc ctgatgatgg gatgtgggtc
     2521 atacctattt gcttcgggca tagctgggag caagggaaat gaaggggacc gacccgggaa
     2581 agcccaattg caaaatcatc tcaaatcggg caatgcagta tcgcttaact ggcagagtct
     2641 ggggcctgat a
//
LOCUS       DAORPD010000149         2637 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_6959_length_2637_cov_2.335012, whole genome shotgun sequence.
ACCESSION   DAORPD010000149 DAORPD010000000
VERSION     DAORPD010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6959_length_2637_cov_2.335012"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1545)
                     /locus_tag="PK976_02515"
     CDS             complement(<1..1545)
                     /locus_tag="PK976_02515"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HRC66792.1"
                     /translation="MNTIEIICQNCDERREFPKGITLAEVSRQFNVKLKYPILGALVN
                     NKLRELSFDLYKPKTVNFIDFSHNIGYRMYVRSVYFLLYAAVKEVIPQALLRIDHSVS
                     KGWYAELEGENIELNEDLLKRIRERMVEMVAADLPFTRQEILTAEAAQLFMKNGQMDK
                     AELFGRRHEMYTSVYGLNGFINYFYGYLVPSTGYLTLYDIRHYYKGLLIRVPDPENFE
                     IPPAVTHQPKLFKVFREHKKWINILDVPFVGNLNKATSEKRDTDLIKISEALHEKRIA
                     RIADKIYRWYDKVKIVLISGPSSSGKTTFSKRLSIQLMVLGLKPVQISIDNYFVDREH
                     TPRDEKGEYDFESLQAIDIDLFNENLNDLLIGRDVRLPRFDFNSGRRSYYNGTFKMKP
                     DQVLIIEGIHGLNPDLTPHIDPARKFGIFVSALTQISIDAQNPISSTDNRLFRRMVRD
                     YRFRGYSALETLRRWDSVRNGEEKNIFPFQENADVMFNSALLYELAVLKTYAEPLLKT
                     IPEIEPE"
     gene            complement(1556..>2637)
                     /locus_tag="PK976_02520"
     CDS             complement(1556..>2637)
                     /locus_tag="PK976_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016276574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRC66793.1"
                     /translation="LLQSNSLTCQYLSGKKKIEVPPKRRLGNGQSLILKGARGNNLKN
                     INLKIPLGKFICVTGVSGSGKSTLIEETLYPVISQALYRSEQKPLPYDSLEGLKNIDK
                     IIQIDQSPIGRTPRSNPATYTKVFDEIRQLFARLPESQVRGYKPGRFSFNVRGGRCEA
                     CGGAGVRVIEMNFLPDVTIPCEVCQGKRYNRETLEIRYKGKSINDVLNMTIDQAVEFF
                     ANIPSILRKIQTLKQVGLGYITLGQSSTTLSGGEAQRVKLSSELSRRYTGKTLYILDE
                     PTTGLHFEDVRVLLDVLQKLADKGNTIIVIEHNMEVIKVADHIIDLGPEGGDQGGYIV
                     AEGTPEEVAQNPKSYTGFYLKQVFS"
BASE COUNT          744 a          553 c          547 g          793 t
ORIGIN      
        1 ttcgggttcg atttcgggaa tagttttcag aagtggttcg gcataagttt tcaaaacggc
       61 cagttcataa aggagtgccg aattgaacat cacatcggca ttttcttgaa aagggaagat
      121 atttttttct tctccattcc taacgctatc ccaacgtctt agggtttcga gggcagaata
      181 gcctctgaag cgataatcgc gtaccatcct gcgaaaaaga cgattatcgg tagatgagat
      241 tggattttga gcatcaatag aaatttgggt aagagccgaa acgaaaatcc cgaatttacg
      301 tgctggatct atgtggggag tcaaatcagg gttcagtcca tgaatgcctt ctataatcag
      361 cacctgatcg ggcttcattt taaaggttcc attataatag gaacgcctac cgctattgaa
      421 atcgaaacga ggaaggcgca catcgcgtcc aatgagtaaa tcattgaggt tttcgttaaa
      481 aagatcgatg tctattgcct ggagactttc gaaatcatat tcaccttttt cgtcccttgg
      541 agtgtgctct ctatcgacaa aataattgtc gatagaaatt tgaactggtt ttaagccaag
      601 caccattaat tgaatggaca gcctcttgct gaaagtagtt tttcccgatg aagaaggacc
      661 agaaatgaga acaatcttta ctttatcgta ccatctatag attttatcgg ctatgcgtgc
      721 tatacgtttt tcgtgcaggg cttccgaaat tttaatcaaa tcggtatcac gtttttcgga
      781 agtagctttg tttaaattgc caacaaaggg aacgtcgagg atatttatcc atttcttatg
      841 ttcacgaaat accttgaaaa gtttgggttg atgagtaact gccggaggaa tttcgaaatt
      901 ttcgggatcg ggcacgcgta tcagcaaacc cttatagtaa tggcgaatat cataaagagt
      961 aagatacccg gtcgaaggca ccagataacc atagaaataa ttgataaaac cattcaatcc
     1021 atataccgag gtgtacattt cgtgtcgtct tccaaagagt tcggccttat ccatctggcc
     1081 attcttcatg aagagttggg cggcttcggc tgtaagtatc tcctggcggg taaaaggtaa
     1141 atcggccgct accatttcga ccatcctttc tcttatccgt tttagtaaat cttcattcag
     1201 ttctatgttt tctccttcca gctcggcata ccaacctttg ctcacgctat gatcgatacg
     1261 caacaaggct tgaggaataa cttcctttac cgcagcatac aacaggaagt aaactgaacg
     1321 aacatacatg cggtaaccaa tattatgcga aaaatcaata aaatttactg ttttgggttt
     1381 ataaagatcg aagctgagtt cacgcaattt attattgacc agtgccccca aaatcggata
     1441 cttcagcttt acgttaaact ggcgagacac ttcagccagt gtaatccctt tgggaaactc
     1501 tcttctttcg tcgcaattct gacaaataat ctcgatagtg ttcatgatag atagattaag
     1561 aaaaaacttg ttttagataa aaaccggtgt aacttttggg attttgagct acctcttcgg
     1621 gtgtaccttc ggccacgata tatcctcctt gatctcctcc ttcaggacct aaatcgataa
     1681 tatggtcggc tactttgata acttccatgt tatgttcgat cacgataatg gtattgcctt
     1741 tgtcggctaa tttctgaaga acatccagta aaactcttac atcttcgaaa tgaagtcctg
     1801 tagtaggttc atcgaggata taaagggttt tcccagtata gcgacgcgaa agttcggaag
     1861 atagcttcac tcgttgtgct tccccacccg aaagagtagt cgacgactgc ccaagagtaa
     1921 tataacccag tcccacttgc ttcaatgttt gtattttgcg aagaatggaa ggaatattgg
     1981 caaaaaattc aacggcctga tcgatcgtca tattaaggac atcgttgata gattttccct
     2041 tgtatctgat ttcgagggtt tcgcgattgt atcgtttccc ttggcatact tcacagggaa
     2101 tcgtaacatc aggtaaaaaa ttcatttcta taactcttac acctgcacca ccacaggctt
     2161 cgcatcgtcc tcccctcacg ttgaacgaaa accttccggg cttgtaacct ctcacttgtg
     2221 attcaggcaa gcgtgcaaaa agctggcgta tctcgtcaaa aaccttggtg tacgtggcag
     2281 gattgctccg gggagttcga cctataggtg attgatcgat ctgaatgatc ttgtcgatgt
     2341 ttttcaaacc ttcgagacta tcgtatggta gaggtttttg ctcggaacga tagagagcct
     2401 gactgataac cggataaaga gtttcttcga taagggtaga ttttccacta cccgaaacac
     2461 cagtaacgca aataaatttt ccaagaggaa tcttcaaatt aatatttttc agattatttc
     2521 ctctagcgcc cttaagaatg aggctttgcc cattaccaag ccgccgctta gggggaacct
     2581 ctattttttt ctttccactg aggtattggc aggtaagtga attactttgg agcaatt
//
LOCUS       DAORPD010000150         2619 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_7030_length_2619_cov_2.053432, whole genome shotgun sequence.
ACCESSION   DAORPD010000150 DAORPD010000000
VERSION     DAORPD010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7030_length_2619_cov_2.053432"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(586..1410)
                     /locus_tag="PK976_02525"
     CDS             complement(586..1410)
                     /locus_tag="PK976_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66794.1"
                     /translation="MKSLSKLVMILLGGMTLAGCSPYRELAQQYVDQSSKRAVVYLIP
                     TGYVDKVNLKRYPTLDLSAYTQYQRDSIRFEKSIFLKNLSDSIFLTRFVNSYMEELRA
                     LGVRVCLDGPDSLTCLPAEDSWIVIMDHLELREFNIPITDEQYIYPALYQKDIDLEAV
                     SLHALFSINRLNEDKKEQQLYAENYVSDEITESGFGFEKDKDKVVHYRKVQPLTLEMI
                     YNMAEALGRKYASYTYDYLLNEYIFKSSKSVPSTYYHYNRFKNKLEEAGDDRFIAK"
     gene            complement(2072..>2619)
                     /locus_tag="PK976_02530"
     CDS             complement(2072..>2619)
                     /locus_tag="PK976_02530"
                     /inference="COORDINATES: protein motif:HMM:NF012870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HRC66795.1"
                     /translation="QQYVQFTQPLPSEPFEKIKMDIKFIYIRGQRKNALLLTFLDTFT
                     RLPLAWDFQYSIRHQNVAMLFDRIIEDWLQPYRPCIDGKVRVCLRSDNDSRFVAKDLQ
                     DYLKSNFVDQEFILPATPQQNAHIESFNHVVEELVCKKYEFDDISQGRQVLNRFFYTY
                     IFAGLSVQYYTCRRQCFLMNG"
BASE COUNT          809 a          442 c          551 g          817 t
ORIGIN      
        1 cttggacgag ttgcctttag aatatatctt ccaatcaaag aaaagcatcc tgctatgata
       61 gtaacaatat aaaccaggaa taaatattcg agttcattgg gttgtcgctg aaatccgaaa
      121 tcataaataa atacaagaat tgatatcgaa ctcagccaaa atggtatgcg caggagtttc
      181 tcgaaacttg aaaatttttt gttttttcga aaaatgaaag aattattcac tgaaacaaaa
      241 tcttttcatg atcacatcag tcgtgataat attgcataat attcttcttg ccgaaaaaag
      301 aatgtaaacc attgcaatat tacgaaattt atccttcgat tttatgatgg ttcatattta
      361 ctctctgttt tcatcgatat cggagtaatt gcccttctgt cccttgttac tcgagaatat
      421 ggcaaatttg cagtgtcatg ttataactgt attcttagtc tgcgtaaaag tgcacatcca
      481 tacttcgggg aaaaatcaac cttcttttca acgagaaaca gcagtaaaat gggcgaatag
      541 cgctctttct tcttcttcaa tgttcaggtg tcgagaggca gaggtttatt ttgcaatgaa
      601 gcgatcatcg cccgcttctt cgagtttatt cttgaacctg ttataatgat aataagtgga
      661 aggcactgat ttgctgcttt tgaagatata ttcattcaac aggtaatcat aggtataaga
      721 agcgtattta cgacccagag cctcggccat gttgtagatc atttcgagag tcaagggttg
      781 tactttgcga taatgaacta ctttatcctt atctttttcg aacccaaagc cactctcggt
      841 gatctcatcg cttacgtaat tctcggcata taactgttgc tcctttttgt cttcattcaa
      901 acggttaatc gaaaacaagg catgcaagga gaccgcctcc agatcgatat ctttctgata
      961 taaagcagga taaatgtatt gttcgtccgt tatggggata ttaaactctc gtaactcgag
     1021 atgatccata attactatcc atgaatcttc tgctggaagg caagtgagtg aatcgggacc
     1081 atcgaggcag actcgtacgc ctaaagctct aagctcttcc atgtaagaat ttacaaatcg
     1141 agtcagaaaa atcgaatcgg ataaattctt caagaaaata ctcttttcga atctgatgct
     1201 atcacgctgg tactgtgtat atgccgataa atcgagagtc ggatatcgtt tcagatttac
     1261 tttatcgacg tatcctgtag ggattaaata aacaacagcg cgtttcgacg attgatccac
     1321 gtattgttga gccaactccc ggtagggact gcaccctgca agtgtcatcc ctccaagcaa
     1381 tatcataacc aatttcgata gacttttcat agcactatcc ttttgtttta tggatttgaa
     1441 ataatgcact tcaagtaata aaaagaacct tctaatttcg tgtcaaatat gcaaaaaatt
     1501 tgccagagat gtcagaatgg ggacagtggg actgaaaata gggaatgaag gaatggaggg
     1561 atggagacga ggggatgaga ggatggtgaa tggtgaatgg cgattagtga gttaaaagtt
     1621 gaaagtttat aaaggtaaaa acgaaagaag ggaggaaggg aggaagggag gaagggagga
     1681 agggaggaag ggagacgtgg ggactaggag actgagagac gaggtggatt ggtgatggtg
     1741 attggtaagt taaaatttat aaagttaaaa gtggagaaag ggggacgagg agacagagag
     1801 actggaattt gggatttcga atttgtaatt tatagtcgta agttgtatgt tgttaattgt
     1861 aagttgttgg ttgtggctta accccctatt gttagaacta aattagtgat gttttaacaa
     1921 atttatcatc ttttttagta attttgttat taagctcttc ataaaattct tcagacaggg
     1981 cagagagcca ttttggcctc tgtcctctga agaaaaatgt ttgttttgtt ctgttccctt
     2041 tctttttgtt tattaccccg atatttcctt ttcagccatt cattaaaaaa cactgccggc
     2101 ggcaagtata atattgaact gatagtcctg cgaaaatata ggtgtaaaag aatctattca
     2161 gtacctgcct tccttgtgag atgtcatcaa attcatattt tttgcatacc agttcttcta
     2221 ctacatggtt aaaactttcg atatgggcat tctgctgagg ggttgcaggc aaaatgaatt
     2281 cctgatccac gaagttactc tttagataat cttgaagatc ctttgcaaca aatcttgaat
     2341 cattgtcgct acgcaggcat actcttacct ttccatcaat acaaggtcga taaggttgaa
     2401 gccaatcttc aattatcctg tcaaaaagca tagctacatt ttgatgccga atggaatatt
     2461 gaaaatccca tgcaagaggt aaacgggtaa aagtatccaa gaaggtaagt agaagagcat
     2521 ttttacgttg tccacgtatg taaataaatt tgatgtccat tttaattttt tcaaatggtt
     2581 ctgaaggcaa gggctgagta aattgtacat attgctgtt
//
LOCUS       DAORPD010000151         2606 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_7077_length_2606_cov_2.303410, whole genome shotgun sequence.
ACCESSION   DAORPD010000151 DAORPD010000000
VERSION     DAORPD010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7077_length_2606_cov_2.303410"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="PK976_02535"
     CDS             <1..628
                     /locus_tag="PK976_02535"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRC66796.1"
                     /translation="EAQRENRFIRKGTKEYESNKYNEAEIAFRKALEKNPSSAVSQYN
                     LGNSLYKQNNLEESVKAWSSLANRSDLSSQNSAHVWHNIGNAFLKSKKYNEAIEAYKN
                     ALRLNPKDEDTRYNLSYALRMAKKQDQQKQKDQQKQDQQKQDQQKDQQKQDQQQAQQQ
                     PQQGQPQEISKQDAERMLEAMKNAEQKTLEKLKKEKKAIVTEKPEKDW"
     gene            715..2589
                     /locus_tag="PK976_02540"
     CDS             715..2589
                     /locus_tag="PK976_02540"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HRC66797.1"
                     /translation="MKRIKTLIFILSIIVPVFSLKSQNVEFTASAPSAVSQGETFQVT
                     YKVNAKGEKFNGPDFKGFDVVSGPNVSQSSSFQFINGKMSQSNEQIYTYLIHASEVGT
                     FDIKPASIVVEGKTYYSNTLRITVEKSSSGSTSKSPSKPRTTPGRPTEPIQSIDDLSQ
                     EDIFVKAYVSKSNPYVGEQVIITYKLYTKVPLANIRINNLGTFPGFWSQDLIKSDQLQ
                     QSTEIINGQEYVTAEIKKSALFPLKSGTFNIPPLEMEATATLIKQRKRFSTGDPFFDQ
                     FFNDAFFNTGYERVNKKVYSNAITITAKPLPTSGKPSEFSSAVGNFKFSAQVDRTQLK
                     TNEALNLKVTISGSGNLDMIEAPNIKFPTEFDTYDPKVNSNISTSSSGFSGSKTFEYL
                     IIPRTPGKYTIKPFTFSYFNPSTGRYYTETSPEFTIEVLKGAEQPAGITYSGVNQQDI
                     KYIGTDIRYIKLPPFHLRPVGVTFFASKLYYGILIGLIVLFGLLIFGIKTMRKRRGDA
                     GLMRLRKATSVATKRLKTAHTYLKNQQEAQFYNEISAALWGYISDKFNIPMAELSMEN
                     VENKLLQKNVDPQTIKAFIDTLNNCEFARFAPGNKSENMEQVYKEALSTISRIEQELK
                     "
BASE COUNT          885 a          538 c          484 g          699 t
ORIGIN      
        1 tgaagcgcag cgggagaatc gttttatacg taaaggaaca aaagagtacg agtcgaacaa
       61 atataatgaa gccgaaatag cctttcgtaa ggctcttgaa aaaaatcctt cttctgccgt
      121 atctcagtat aatcttggca attcacttta taagcagaac aatctcgagg aatcagtaaa
      181 agcatggagc agtcttgcca atcgtagtga cctttcatcc cagaattcgg ctcatgtttg
      241 gcataatata ggaaatgcat ttctaaaatc aaaaaaatat aatgaagcca tagaggctta
      301 taaaaatgct ctgcggctca atcctaaaga tgaggacacg cgctataacc tgtcgtatgc
      361 gctcagaatg gcaaagaaac aagatcagca gaagcaaaaa gatcagcaga agcaagatca
      421 acaaaagcaa gatcaacaaa aagatcagca gaagcaagat cagcaacaag cccaacagca
      481 gcctcaacaa ggtcaaccac aagaaatttc gaaacaagat gccgaacgta tgctcgaagc
      541 catgaaaaat gccgaacaaa aaactttaga aaaacttaaa aaagaaaaga aagctatagt
      601 taccgaaaaa ccagaaaaag attggtaaag gcataaattt tgtgccaccg tttccggctg
      661 aattacttac gtttgtgata taattttatt aaaaatagaa agctcgaccc aaacatgaaa
      721 agaataaaaa ctctgatatt cattttgtct atcattgttc cggttttttc gctaaaaagt
      781 cagaatgttg aatttacggc ttcggctcct tctgccgtaa gccaaggtga aacgtttcag
      841 gttacttaca aagtcaacgc taaaggggaa aagttcaacg gccctgattt caaaggattt
      901 gatgtagttt cgggcccgaa tgtctcacag agttcgagtt ttcagttcat caatggtaaa
      961 atgagtcaat cgaacgaaca aatttatact tatctcattc atgcttccga agtaggaact
     1021 tttgatataa agccagcaag cattgtagtg gaaggaaaga cttactattc gaatacactg
     1081 cgaattacgg tagaaaaatc ttcgagtggg agcacttcaa aaagtccatc taaaccgcgc
     1141 acaacacctg gcaggcctac cgaacccatt caaagcattg acgacctctc acaagaggat
     1201 atcttcgtaa aggcttatgt tagcaaatct aatccttatg ttggcgaaca ggtaattatt
     1261 acttataaac tttataccaa agttcctctt gctaatattc gcatcaataa tttgggaacc
     1321 tttcctggat tttggtcaca ggatcttatt aaaagcgacc aattgcagca atctaccgaa
     1381 attatcaatg ggcaggaata tgttaccgca gaaattaaaa agagtgctct tttcccttta
     1441 aaatcaggaa cttttaatat acctccactc gaaatggagg cgactgctac cttaattaaa
     1501 cagcgcaaac ggttttctac aggcgatcct ttttttgacc aatttttcaa cgatgctttt
     1561 ttcaatacag gctatgaacg cgtcaataag aaggtttaca gtaatgccat taccatcact
     1621 gctaagcctt tacccacaag cggtaaacct tccgaattta gttcagctgt gggtaacttc
     1681 aaattttcgg ctcaggtcga tcgtactcag ttaaaaacca atgaagcgct caatctcaag
     1741 gttactatta gtggcagtgg gaatcttgac atgatagagg ctcccaatat caaattccct
     1801 accgaattcg atacttacga tccaaaggtt aattccaaca tctctacttc ttcttcgggg
     1861 ttttcgggta gcaaaacctt cgaatacctc attataccac gaacacctgg aaaatatact
     1921 ataaaacctt tcacctttag ctattttaat ccttcgacag gaagatatta taccgaaacc
     1981 tcgcctgaat ttaccataga agtgctcaaa ggcgcagaac agcctgcagg aatcacctat
     2041 agtggagtaa atcagcaaga tattaaatat attggaaccg atattcgtta tatcaaatta
     2101 cctccctttc atctgcgacc tgttggagta actttcttcg cttccaaact ctattatgga
     2161 atattaattg gcttaatagt attgtttggc ctcttgatat ttgggataaa aaccatgcga
     2221 aagcgcagag gggatgccgg gttgatgcgt ttgcgcaaag ctactagtgt ggctaccaag
     2281 cggttgaaga cggctcatac ctatctcaaa aatcagcagg aagcccagtt ctacaatgag
     2341 atatccgctg ccttgtgggg ctatatctcc gacaaattca acattcctat ggcagaactt
     2401 tcgatggaaa atgttgaaaa taaacttctt cagaagaatg tcgatcctca aactataaaa
     2461 gctttcatcg atacgctcaa taattgtgaa tttgcccgtt ttgctcctgg aaacaaatcc
     2521 gagaacatgg aacaggttta caaagaagct ctttccacta tctcacgtat tgagcaggaa
     2581 ttaaaataaa gttcataata aattag
//
LOCUS       DAORPD010000152         2597 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_7117_length_2597_cov_2.599135, whole genome shotgun sequence.
ACCESSION   DAORPD010000152 DAORPD010000000
VERSION     DAORPD010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7117_length_2597_cov_2.599135"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="PK976_02545"
     CDS             complement(<1..603)
                     /locus_tag="PK976_02545"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HRC66798.1"
                     /translation="MSIITLTSDWGLKDHYLSVVKAAILSRLPETIIIDISHQIPPFD
                     LNQASFIIRNCWKGFPKGTVHIIGINTEAGIKSPHTAVEMEGHYFIGADNGIFSLIFD
                     KQPERIIEIEIAQDSEYFTFSTRDVFVKAAVHLLKGNSIEDLGIPRNSLTPCASFQPV
                     ITQNAITGKVIYVDVYENVFTNISREMFQQVIKGRHFQIEL"
     gene            705..1664
                     /locus_tag="PK976_02550"
     CDS             705..1664
                     /locus_tag="PK976_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010264372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRC66799.1"
                     /translation="MNERIISIESYSPLEIYGPNDQYLNLIRDRFPQLKIIARGDVIK
                     IIGEKSQIDLFDRLFGLLLVHYDEFGKITENDLFEIFSNEGEEEFNQVRPIEGNDILV
                     YGNHGLQVRARTINQRLLVESSQKNDMVFAIGPAGTGKTYTAVALAVKALKNREVKRI
                     VLTRPAVEAGENLGFLPGDLKDKLDPYLQPLYDALRDMLPTQKLLTYLEDGTIEVAPL
                     AFMRGRTLDHAFAILDEAQNATLNQLKMFLTRMGRSAKFIITGDITQIDLPPSQKSGL
                     VHATQILKGIQGIDFVFLDQRDIIRHRLVKDVIEAYGRDNPQH"
     gene            1722..>2597
                     /locus_tag="PK976_02555"
     CDS             1722..>2597
                     /locus_tag="PK976_02555"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HRC66800.1"
                     /translation="MYNAVLETNYHFPGQTKIYYGKVRDVYFVDDLLVMIATDRISAF
                     DVVLPRAIPYKGKVLNQIAAYFLDATSDIVPNWKIATPDPMVTTGYLCKPYPVEMIIR
                     AYLTGSSWRAYLKGARQICGVPLPEGMKEHQPFPQPIITPTTKATEGHDMDITRNEII
                     QNGLIPEEDYLQIEEYTRRLFERGSIMAAQKGLILVDTKYEFGNRDGKIYLIDEVHTP
                     DSSRYFYAEGYQERFAKGLPQLQLSKEFVREWLMERGFMGQIGQRVPDMDDDFVNSVS
                     ERYIELYEKITGEKFV"
BASE COUNT          794 a          554 c          522 g          727 t
ORIGIN      
        1 caattcgatc tggaaatgtc ttccttttat cacttgctga aacatttcac gacttatgtt
       61 agtgaaaaca ttttcataaa catctacata aataacctta cccgtaatag cattttgagt
      121 aataacaggt tggaaggaag cacatggtgt taggctattt cgaggtatac ccaggtcttc
      181 aatagaattg ccttttaata gatgcactgc ggcttttaca aagacatccc tcgtcgaaaa
      241 agtgaaatat tcactatcct gcgctatttc gatctcgata atgcgttcgg gctgcttgtc
      301 gaaaatcagg ctgaaaatcc cattatccgc accgataaaa taatgccctt ccatttccac
      361 tgcggtatgt ggcgatttaa tcccagcctc ggtgttaatt ccaataatat gcactgtacc
      421 tttgggaaaa cccttccaac aatttcttat gataaacgaa gcctgattta aatcgaatgg
      481 cgggatttga tgactgatgt caataattat cgtctcgggc aggcgactta agattgccgc
      541 ctttaccact gatagataat gatcctttag tccccagtcg cttgtaagtg ttataattga
      601 catttaaaat tatggtgaat gcgtggtttt ttactgtaat tttgcaaatc ccaaaattac
      661 aaaaatcaca gttttatatt ttaagagtag cccaatcaag ctgaatgaac gaaagaatca
      721 tttccatcga atcttatagc ccgctcgaaa tatatggtcc taacgaccag tatctaaacc
      781 taataagaga tcgttttcct caattgaaaa ttatagccag aggcgatgta atcaagatta
      841 ttggagagaa atcgcaaatt gatcttttcg atcgactttt tggactctta ttggtacatt
      901 acgatgaatt tggcaaaatc accgaaaatg atcttttcga gatttttagc aacgaaggag
      961 aagaagagtt taatcaggtt aggccgatag aagggaatga tattttggtt tatggaaatc
     1021 atgggttaca ggtaagggcg cgtactatca atcagcgatt gctggtcgaa agcagccaga
     1081 agaacgacat ggtatttgcc attgggccgg caggcacggg aaaaacctat actgcggttg
     1141 cacttgccgt taaagcttta aaaaataggg aagtaaagcg aatcgttctg acacgtcctg
     1201 ccgtcgaagc tggtgaaaat cttggcttcc tccccggtga tttaaaagat aaactcgatc
     1261 cttatcttca gccactttac gatgcactgc gcgatatgct ccccacgcag aaattgctca
     1321 cctatctcga agatggaacc atcgaagttg cccccctggc ctttatgaga gggcgtaccc
     1381 tcgaccatgc ctttgctatc ctcgatgaag cacaaaatgc cactcttaac caattgaaaa
     1441 tgttcctcac gcgtatggga cgcagtgcca aatttatcat tactggcgat atcactcaga
     1501 tcgaccttcc tccatcccaa aaatcgggcc ttgttcatgc cactcaaatc ctcaaaggca
     1561 ttcagggaat tgacttcgtc tttcttgacc aacgcgatat tatccgacac cgtctggtaa
     1621 aagatgttat agaagcttac ggccgtgata accctcaaca ttaaaaaaac aataaaaacg
     1681 aaaccccact ttaattcatc attatacttt ttttaaaaaa tatgtataac gcagttcttg
     1741 aaacgaatta ccacttcccc ggacaaacaa aaatatatta tggcaaggtg cgcgatgtct
     1801 attttgtgga tgatctactg gtgatgatag caaccgatag gatttcggcc ttcgacgttg
     1861 ttttgccaag agccatacct tacaagggaa aggttttgaa ccaaatcgcc gcctactttc
     1921 tggatgccac gtccgacatc gttcccaatt ggaagattgc cacccccgat cccatggtta
     1981 ctacaggtta cttatgcaaa ccgtatcctg tagaaatgat catccgggct tatttgacag
     2041 gaagttcatg gagagcttat ctaaaaggag cccgacaaat ttgcggagtt cctctgcccg
     2101 aaggcatgaa agaacatcaa ccatttccac agcctatcat aactcccact actaaagcca
     2161 ctgaaggaca cgatatggac atcactcgca atgaaatcat ccaaaatgga ttaattcccg
     2221 aagaagatta tctacagata gaagaatata ctcgccgcct tttcgaacga ggttcgataa
     2281 tggctgcaca aaaaggactt attctggtgg atacaaagta tgagtttggc aatagggatg
     2341 gcaaaatcta cttgatcgat gaggttcata ctcccgattc atcgcgatat ttttatgccg
     2401 aaggttacca agaacgtttc gctaaaggtc ttccacagtt gcagctttcg aaagaattcg
     2461 taagagaatg gcttatggaa agagggttca tgggtcagat cggacagcga gttcctgata
     2521 tggatgacga ttttgtaaat agcgtgtcgg aaaggtatat cgaactttat gaaaaaatta
     2581 caggagaaaa atttgtc
//
LOCUS       DAORPD010000153         2595 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_7123_length_2595_cov_2.266142, whole genome shotgun sequence.
ACCESSION   DAORPD010000153 DAORPD010000000
VERSION     DAORPD010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7123_length_2595_cov_2.266142"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="PK976_02560"
     CDS             complement(<1..249)
                     /locus_tag="PK976_02560"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylase"
                     /protein_id="HRC66801.1"
                     /translation="MPRPNASELVLSADGNIYHLRIKPEQLADTVILVGDPGRVSMVS
                     SHFDRLEFQGQNREILVHTGWIGKTGISVISTGMGPDNI"
     gene            529..2223
                     /gene="ispG"
                     /locus_tag="PK976_02565"
     CDS             529..2223
                     /gene="ispG"
                     /locus_tag="PK976_02565"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007479874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HRC66802.1"
                     /translation="MEKYPFYIQKDYQRLTTIEIPVGDIPVGGTNPIRIQSMTNTFTM
                     DTEATVAQAIRLFDAGCDYVRITAQGPKEASNLKAIKVELHLRGYTKPLIADIHYSPK
                     AAEIAAAVVEKVRINPGNYIGPHMGKITYTDKEYREELEIIYDHIKPLLEICRQYGTA
                     LRIGSNHGSLSKRILSRYGDTPEGMVASAIEFIEICEDLDFDHIVLSMKSSNPRIMIE
                     ANRLLAMTMLKRGKVYPVHLGVTEAGDGEDGRIKSAAGIGALLAEGIGDTIRVSLTEE
                     PEAEIPVASILKEMTSVFPLNPLAFGLKNYRYENFQKEKFVSNEIHEIGGMNPPLVIG
                     QTSSADIVILSDLPFRTHQIHTQSFPSHLEIARLQEELPIVIHSDHPRELIALRAAVI
                     HLRNQGMNQPIIIKRSSYEAKPEKFITESAAVMGPAFQEGLGQAIWLENPKLKPEQLV
                     NISFSILQSVKARISRTEYIACPSCGRTLFNIQPTLRSIKERTSHLKGLTIAVMGCIV
                     NGPGEMAGADYGYVGAGRGKITLYKGHAPVKRNIPEEMAVDELIDLIRQNGDWIEP"
     gene            2235..>2595
                     /locus_tag="PK976_02570"
     CDS             2235..>2595
                     /locus_tag="PK976_02570"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRC66803.1"
                     /translation="MYCYHTHTYFCDGTSAPELYVKNALQLHFYSLGFSGHAPVPFAN
                     NFAIPNEKLISYCEEINSLKSKYQNKLSILLGLEMDYIPGVSKPFEELARECHLDYTI
                     GSVHFVRNVAKEELWFID"
BASE COUNT          818 a          535 c          530 g          712 t
ORIGIN      
        1 gatgttatct ggccccatcc cggtcgaaat aaccgatatt cctgttttgc ctatccaacc
       61 cgtatgaacc aatatctcgc gattctgtcc ttgaaattcc aaacggtcga aatggcttga
      121 aaccattgat acccgaccag gatctccaac caaaattacc gtatcggcaa gctgctcggg
      181 ctttatcctt aaatgataaa tattcccatc agcactgaga acaagttcgg aagcgttggg
      241 tcttggcata aacctaaaat atttatcgat aaacagaaaa gaatatagaa aattttagag
      301 aggcaaaact aaagataaaa aggtcataaa agaagaaaaa gaatttttat caatcatcga
      361 ccactgaacg aagaatgaca tgttagtgag caatctagcc aaacataatg ggtaatgtga
      421 ataaggctgc aattcgaatt tatttctttc caagtcgtca ggaaggtttt atgtcgaatc
      481 ttttatatta gacaggaatg attttaattt tgcagaaaat gcatatctgt ggaaaaatat
      541 ccattctata ttcaaaaaga ttatcaaaga ttaacgacca ttgaaattcc cgttggggat
      601 ataccggttg gaggaacaaa tcccattcgt attcagagca tgaccaatac ttttacgatg
      661 gataccgaag ccactgtcgc tcaggccatt cgattgttcg atgcaggttg tgattatgtg
      721 cgtatcacgg cccagggacc taaagaagct tcgaatctaa aagcgataaa agtagagctc
      781 cacctaagag gatatacaaa gcccttaata gccgacattc attacagtcc gaaagccgcc
      841 gaaattgccg ctgctgttgt cgaaaaagtg agaatcaatc caggcaatta cattggcccg
      901 cacatgggaa aaattactta taccgataag gaatatcggg aggaattaga aatcatatac
      961 gatcatataa aacctttatt ggaaatatgc cgtcaatatg gaactgcatt gcgtattgga
     1021 agcaatcacg gatcgctgtc gaagcgcatc ctttctcgtt atggagacac acccgaagga
     1081 atggtagcct cagccatcga gtttattgag atttgcgaag acctcgattt cgatcatata
     1141 gttttatcga tgaaatcgag caaccctcgc atcatgattg aagccaatcg tttacttgca
     1201 atgacgatgt tgaagagagg caaagtttat cctgtacatc tgggtgtaac cgaagcaggt
     1261 gatggagaag atggaaggat aaaatcggct gcaggcatag gagctttact tgccgaaggg
     1321 attggcgata ctattcgcgt gtcattgacc gaagaacctg aagccgagat tccagtagcc
     1381 tctatactca aagaaatgac cagtgttttt ccgttaaatc ctcttgcatt cggtttaaaa
     1441 aactatcgtt atgagaactt tcagaaagaa aaattcgtta gcaatgaaat acatgaaatc
     1501 ggcggtatga atcctccttt agtgattggt cagacatcat ctgcagatat cgttatttta
     1561 tccgacctcc catttcgaac ccatcagatt catacccaat cgtttccatc tcaccttgag
     1621 attgcaagac ttcaggaaga attgcccata gtgattcatt ccgaccatcc tcgtgaattg
     1681 attgccctga gggcagcagt tatacatctg aggaatcagg gtatgaatca gcctattatt
     1741 ataaaaagaa gctcttacga ggccaaacca gagaaattca ttaccgaatc cgctgcggta
     1801 atgggacctg cattccagga aggattggga caagccatct ggctcgaaaa tcccaaatta
     1861 aagccagaac agctggtcaa catttccttt tccatccttc aatcggttaa agcacgcatc
     1921 tcccgtaccg aatacattgc atgcccttcg tgtggccgaa cactttttaa tattcaaccc
     1981 acacttcgaa gcatcaagga gcgcacttct catctcaaag ggcttactat tgctgttatg
     2041 ggttgcatag taaatggacc tggtgaaatg gccggagccg attatggcta tgtaggtgca
     2101 ggtagaggaa aaattactct ttataaagga catgctcctg tgaaaagaaa tattccagaa
     2161 gaaatggccg tggatgaatt gatcgattta atccgtcaga acggcgattg gatagaacct
     2221 taaagccttt gtcgatgtat tgttatcata cccataccta tttctgcgat ggaacttccg
     2281 ctcctgaact ctatgtaaaa aatgcccttc agcttcattt ctattcttta ggtttttcgg
     2341 gacatgcacc tgtgccattc gccaataatt ttgctatacc caacgaaaaa cttatatctt
     2401 actgtgaaga aataaattca ctcaaatcga agtatcaaaa taaactgagt attttattag
     2461 gtttggaaat ggattatatc cccggagttt ccaaaccctt cgaagaatta gccagggaat
     2521 gccatcttga ttataccata ggttcggttc atttcgtgag aaatgttgca aaggaagagc
     2581 tatggttcat cgatg
//
LOCUS       DAORPD010000154         2594 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_7127_length_2594_cov_2.103978, whole genome shotgun sequence.
ACCESSION   DAORPD010000154 DAORPD010000000
VERSION     DAORPD010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7127_length_2594_cov_2.103978"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            191..1573
                     /locus_tag="PK976_02575"
     CDS             191..1573
                     /locus_tag="PK976_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRC66804.1"
                     /translation="MSKSNRRDFIKITMGGLAAAGLLKHSGGLRSKPILMQVPERKGS
                     TVMGMTSKALPTVRIGVIGLGMRGSEAVTRLLQVEGTEIVALCDLLPERVEMAKEVVR
                     KQRGTEPVGFFSGEEEWEKLCRRDDIDLVYICTPWEWHTPMAVDAMRNGKHAAVEVPA
                     ALTIEECWELVDTSEATRQHCIMLENCCYDFFELNTLVMARRGLFGEIMHAEGAYIHD
                     LRWLKFDEQNGYYPMWRLEYSKFHTGNPYPTHGLGPIAQIMGINQGDCMEYLTSMSTN
                     QVGMSLYAKETFGPDSKFAKPEYRLGDMNTTLIRTINGKTMMIQHNTTSPRPYSRIHL
                     ISGTKGIAQKYPEERLAFEPEAHRWLNDKEMKKVMEDNQHPLIKQIGEKARKVGGHGG
                     MDFIMDYRLIYCLRNGLSLDETVYDAATWSSLVELSEISVKNNGQPVAIPDFTRGEWE
                     HTPPLGIVTL"
     gene            complement(1627..2472)
                     /locus_tag="PK976_02580"
     CDS             complement(1627..2472)
                     /locus_tag="PK976_02580"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRC66805.1"
                     /translation="MQKNTAMPILIIVDFSDNSLEAAIQAANLAELLHEDLLLVHAVN
                     HYTRAYLHENNLDSEWIEESMEWLSQKLTRGREIDVEFVIREGSVYEVISKISRESNA
                     LLIAFSLKGKPGLQKFTPEKIMRMVAASPIPSIIFPRHVCNKFSPMLFPVNLFRGWAK
                     KAKALTHLSQWFGKQVIVVENHSSVMFASEIERERYHFSEKLNSFGLECTFASYLLQG
                     SYTNQLAYYAKRYKAGLLVLMNDEEEESLHFRPGLSETKIIRSKIGLPVLCVSPYMFS
                     QDESR"
BASE COUNT          796 a          490 c          569 g          739 t
ORIGIN      
        1 ttataattgc tcattataag ccatttttga aaaaagaatt atgagattta tagaaaaaat
       61 ttagcatgaa ttggctgaat tggatagttt gagctgtttg tcagagaaca ataagatagg
      121 tggagaaaga attccgaccg aaataaggaa aaaattttga aattagcgcc aataaattat
      181 cctaatcatt atgagtaaga gcaatcgcag agattttatc aagattacca tgggaggact
      241 tgctgctgca gggcttctta agcattcagg gggtttaagg tcaaaaccca ttcttatgca
      301 ggttccagaa aggaagggaa gcacggtaat gggtatgacg agtaaggcct taccaactgt
      361 aagaatcggt gtgattggat taggtatgcg cggcagtgaa gctgttacga gattattgca
      421 agtggaagga accgaaatcg ttgcattatg cgatttattg cccgaaagag tagaaatggc
      481 caaagaagtg gtaagaaaac aaagaggcac tgaacctgtg ggcttttttt cgggggaaga
      541 agaatgggaa aaactttgcc gccgcgacga tattgatctg gtttatattt gtactccgtg
      601 ggaatggcat accccaatgg ccgtcgatgc catgcgaaac ggaaaacatg ccgctgtgga
      661 agtgccggca gccttaacca tagaagaatg ctgggaattg gttgatactt ccgaagcaac
      721 acgtcagcat tgcataatgc tcgaaaactg ctgttacgat tttttcgaac tcaataccct
      781 tgtaatggcc cgtagaggac tatttggaga gattatgcac gccgagggag cctatattca
      841 cgatctaaga tggttgaaat tcgatgaaca aaatggatac tatccaatgt ggcgtctcga
      901 atatagtaaa tttcatacag gaaatcccta tcctacccat ggactcgggc ctatagccca
      961 aatcatggga atcaatcagg gcgactgcat ggaatatctc accagcatgt cgactaatca
     1021 ggtggggatg agtttgtatg caaaagaaac ttttggccct gatagtaagt ttgccaaacc
     1081 ggaatacagg cttggcgata tgaatacgac tctgattcgc acaatcaatg gcaaaacaat
     1141 gatgattcag cataatacga ctagtcccag gccttacagt cgtattcatc ttatttcggg
     1201 gacaaaagga atagcgcaaa agtatcccga agaacggctg gctttcgagc ccgaggcgca
     1261 tcgctggctc aatgataagg aaatgaaaaa agtaatggaa gacaatcaac atcccttaat
     1321 caaacagata ggagagaaag ccagaaaagt tggtggccat ggcggtatgg attttatcat
     1381 ggattaccgt ttgatttatt gcctgcgtaa tggcctatct ctcgatgaaa ccgtatatga
     1441 tgctgcgaca tggtcttctt tggtcgaact ttccgaaata tcagtgaaaa ataatggtca
     1501 gcccgttgct attcctgatt ttacgcgagg agaatgggaa catacacctc cactgggaat
     1561 cgttacgctt tgatttttat tgaaatcgtt tttcttcggt gatggtttgt tcccgattta
     1621 ttgtcatcac cttgactcat cttgagaaaa catatatggt gaaacacaaa gtacaggtaa
     1681 accgatctta ctgcggatga tttttgtctc ggaaaggcca ggcctgaaat gtaatgattc
     1741 ctcttcttca tcattcatca atacaagcaa tcccgccttg taacgtttcg cataatatgc
     1801 caattgattc gtatagctac cttgaagtag ataagaagca aaagtacatt cgagcccgaa
     1861 agagttcagt ttttcactaa aatggtatct ttcacgttcg atctcactgg cgaacataac
     1921 gcttgagtga ttttctacaa cgattacctg cttcccaaac cattgactca aatgtgttaa
     1981 tgctttggct tttttagccc atccacggaa tagattcaca ggaaaaagca tgggggaaaa
     2041 tttattgcat acatgccttg ggaaaataat ggatggtata ggtgaagcag caaccatgcg
     2101 cataattttt tcgggtgtaa atttttgcaa acctggtttc cctttcaagc taaaagcaat
     2161 taataaggca ttactttctc tggaaatttt cgaaatcact tcgtaaacac ttccttcacg
     2221 aattacaaat tcgacatcga tttctcttcc tcgtgttagc ttctgagaaa gccactccat
     2281 cgattcttct atccattcac tgtcgagatt gttttcgtgc agatatgccc gcgtgtaatg
     2341 gtttactgcg tgtaccaaaa gcaaatcctc gtgcagcaat tctgctaaat ttgctgcctg
     2401 aatagcggcc tctaagctat tatcggaaaa atcaacaata attaggatgg gcatggccgt
     2461 atttttttgc aagtaacaaa aattttttct attgaagtta tttaccagaa aatatatttg
     2521 ctaattattt cgagtcctgt attatcttat ggttgaatga attaaaaata agaggcatgc
     2581 atagcatggc atct
//
LOCUS       DAORPD010000155         2593 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_7131_length_2593_cov_4.147754, whole genome shotgun sequence.
ACCESSION   DAORPD010000155 DAORPD010000000
VERSION     DAORPD010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7131_length_2593_cov_4.147754"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(318..926)
                     /locus_tag="PK976_02585"
     CDS             complement(318..926)
                     /locus_tag="PK976_02585"
                     /inference="COORDINATES: protein motif:HMM:NF014970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MutH/Sau3AI family endonuclease"
                     /protein_id="HRC66806.1"
                     /translation="MERTEALTKLQDLVGKELHDLAKKYGVTVYRDGKVNKGWAGHVF
                     ERYLQLPINSAQSPNFGSWELKSIPLKYKKNGELTFKETMAITMIDPVNICQKEFEDS
                     HLLAKLKKAIVVARIVGTHVDDPTYIHSVTEFNLSDDMYEAVKADYDLVRNALLDPAQ
                     GFKALTGYMGVYIQPRTKGRGHGSISRAFYARPKFLAQFIEI"
     gene            complement(938..1768)
                     /locus_tag="PK976_02590"
     CDS             complement(938..1768)
                     /locus_tag="PK976_02590"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HRC66807.1"
                     /translation="MFNGDVIDILNALPKECIDLIFADPPYNLSNGGFTCHAGKRVSV
                     NKGKWDKSKGIEEDFEFHYNWIKACRRVLKPNGSLWISGTYHSIYACGFALQKQGWHL
                     INDICWFKPNAAPNLACRMFTASHETLLWAKKDKEAKHFYNYDLVKAGDWNGDFLKKP
                     NKQMRSVWAITTPKNGEKKYGKHPTQKPEALLDRIVLACTQENDIVLDPFCGSATTGV
                     SALKNGRKFVGIDAEKDYLDKIAIPRIKDVFSQIDLFRHNEKDELRVSEKQAEYGMLP
                     "
     gene            complement(1806..2339)
                     /locus_tag="PK976_02595"
     CDS             complement(1806..2339)
                     /locus_tag="PK976_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007411019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66808.1"
                     /translation="MARRNIYKHSIELKKLTVKNSERYEFAKQLENIPYRGYEVEDIT
                     DGRKIVIVKPGGKQAWGKMRKEDYFVFIYNPLEQSLWQITHKQILEDLKQKAEKDKNC
                     TIKILEVFEKVYKGEEPDDILKEISLVNPIGENPEALLKAYKWIWGQEDVNYPKGKGR
                     EMSWAGLKELLENLKGV"
BASE COUNT          731 a          590 c          408 g          864 t
ORIGIN      
        1 atcatacaaa cacaaaatac gcaaaccgtt catttttcgc aatcccaaaa acgccagcca
       61 atcgcgtcct taaacttttc caaaaaacta tacgaccaat gcgccgacaa atatgatatt
      121 cgcaaatctt ctgtcaaaat atccataatt gaaaaatatc ttcaggtatg aaggaaatat
      181 atactataac ggtcaagtct gaggcacgag gtgaatcgaa gcgataaact ttcacaactc
      241 aatacccttt tgaatagtct gaaatctcaa aatcatgcca gagctcacct cgttgcctcc
      301 atctgatggt tagaatttta tatttctatg aactgggcta agaactttgg gcgggcataa
      361 aaagcccttg aaatagaacc atgaccccta ccttttgtcc ttggctgaat ataaacaccc
      421 atgtaaccag tcaatgcttt aaaaccctgt gcgggatcga gcaatgcatt gcgaaccaaa
      481 tcataatccg ccttaacggc ctcatacata tcatctgaaa ggttgaattc cgtaacggaa
      541 tggatataag taggatcatc aacatgagtg cccacaattc ttgccacaac aatggctttc
      601 ttcagttttg ccagcaaatg actgtcttca aattctttct gacaaatatt gacaggatca
      661 atcatggtta tggccattgt ttccttgaat gtaagctcgc catttttctt atacttcaat
      721 ggaattgatt tcagttccca tgaaccaaaa ttgggagatt gagctgagtt gattggcaac
      781 tgcaaataac gttcaaagac atgaccggcc caacctttat ttactttgcc atctcggtat
      841 actgttactc cgtatttttt agcaagatca tgtagttctt taccaacaag atcctgcagt
      901 tttgttaatg cttctgtacg ttccataaag tgtcctctta cggtaacatt ccgtattcag
      961 cttgtttctc gcttaccctc aattcatctt tctcattatg tctaaataga tcaatctgac
     1021 taaaaacatc ctttattctt ggaattgcta ttttatccaa ataatccttt tctgcatcaa
     1081 ttcctacaaa ctttcttccg tttttgagag cagagactcc agtcgtcgca ctcccacaga
     1141 atggatcgag aacgatgtcg ttttcctgag tacatgctaa aacaatacgg tctaataaag
     1201 cttcaggttt ttgtgtaggg tgttttccat attttttctc accatttttg ggagttgtaa
     1261 tagcccagac acttctcatt tgcttatttg gttttttaag aaaatcaccg ttccaatctc
     1321 cagccttgac aagatcgtaa ttataaaaat gtttggcttc cttatctttc ttagcccaaa
     1381 gtaaagtttc gtggcttgcc gtaaacatgc ggcaagccag atttggagct gcatttggct
     1441 tgaaccagca aatatcgtta attaaatgcc aaccttgttt ctgtaaggca aatccgcaag
     1501 cataaataga atgataggtt cctgagatcc acagagaccc attgggttta agaactcgcc
     1561 tgcatgcctt tatccagttg taatgaaact caaagtcttc ttctattccc ttgctcttat
     1621 cccatttgcc tttattgacg ctgactcttt ttccggcatg gcaggtaaag ccaccgtttg
     1681 acaaattata tggaggatcg gcaaaaatta aatcaatgca ctcttttgga agagcattga
     1741 gtatatctat aacatctccg ttgaacaatc ttacattctt ttctgaaaaa tatgctttta
     1801 tcaacttaaa ctcctttcaa gttttccaaa agttctttta gacccgccca tgacatttct
     1861 ctccctttgc ctttcggata attaacatcc tcttgtcccc atatccactt atacgccttt
     1921 aaaagagctt ctggattttc cccgatcgga ttgaccaaag atatttcctt taaaatatca
     1981 tcgggttctt cgcctttgta aactttctcg aaaacttcga gtattttaat ggtgcaattt
     2041 ttgtctttct ctgctttttg tttcaaatct tcaagaattt gtttgtgagt tatttgccaa
     2101 agggattgct ctaatggatt ataaataaag acgaaataat cctccttccg catttttccc
     2161 catgcctgct tgccaccagg ttttactatt acaattttcc tgccatccgt aatatcttca
     2221 acttcgtatc cgcgataagg tatattttcc agttgttttg caaattcata tcgttcagaa
     2281 tttttgactg ttagcttttt taactctatt gaatgtttgt aaatatttcg tcttgccatg
     2341 attgtttctt tcttttaatt ctaacgaaca gggctgaggc acggcgggtt tcggagtgat
     2401 cctctttcac ttcccgtaaa tcttctggaa actcgctaaa cttcaaattc atcgaacagc
     2461 tttagccgtt gcctccagcc gatggttata gtcctgccaa acctttcgtc ttatccaaat
     2521 caaaaatctc atctctctcc caattcacta acccgaaaaa acgctcgcca tttcatcata
     2581 caaacacaaa ata
//
LOCUS       DAORPD010000156         2586 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_7161_length_2586_cov_2.495456, whole genome shotgun sequence.
ACCESSION   DAORPD010000156 DAORPD010000000
VERSION     DAORPD010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7161_length_2586_cov_2.495456"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1614
                     /locus_tag="PK976_02600"
     CDS             <1..1614
                     /locus_tag="PK976_02600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRC66809.1"
                     /translation="MLKIAGKVFLDAQYRKDTTEKLIDSEEKARALLNVVPDAIVLLE
                     SNGNILDINEKAAELIGGSTVNNQKPNYFDLLPEPIRTARQKIFKKVVSDGRRYSHYD
                     TLLNRHYDLTFYPASNLNKTIKRVAIFSRDITDILANQEAITNHNHFLRALLDNIPNP
                     VFYKDTKGVFLGCNNAFLQAFGKTSTDIIGKKICEIFSIENCRQIIQKDEEIISSKQI
                     QSFELEFQYADGLIHNIIINQSPFSQSDGTLGGLVGIMIDISETKQIQHELEQWNIQL
                     NKRVSEELKKVEKQRQMLIQKSKLESLGQLAAGITHEMNQPLTAITMGLENILFHLKS
                     EDITKHYLENKIGTILENIQRINRIIDHVKTFSRNQQNARFTRMNLNEAIQGALLLLK
                     SQFSERNIHFNLQLHPEPLYFLGDRFKVEQVVINLLNNSKDALEQKAHNQSLNFNKTI
                     AIRSFIINSNNIGMEIEDNGTGIPPQLLDHIFEPFFTTKEINKGTGLGLSIVYGIIEE
                     LKGNIEVKSEFGEGTCFTCEFPSYMNTNN"
     gene            1685..>2586
                     /locus_tag="PK976_02605"
     CDS             1685..>2586
                     /locus_tag="PK976_02605"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRC66810.1"
                     /translation="MAEQLNILIVDDEEIIRNELAEYLQNNNFNVFLAEASTEALEVL
                     KANRVDVVFLDIRLPGINGLELLKIIKMEYPEKEVIMISGHGEMQSVIEAMRSGACDY
                     LPKPFSLTEVISAIERTRRFIDLNNRLKEVENSFNLFKDNIRKELGQGFIAQSQAMKK
                     VSELVSKVAKSDPTNVLIMGESGTGKELVARAIHYMSPRKDHYFYAVNSSAITETLFE
                     SEFFGHTKGSFTGANENKTGWFEVANKGTLFLDEIGDLPLSLQTKFLRVLEERKISRV
                     GGTQEIGIDVRIIAATNQDFEKLT"
BASE COUNT          890 a          504 c          490 g          702 t
ORIGIN      
        1 atgctaaaaa tcgctggtaa ggttttttta gatgcacaat accgaaagga taccaccgaa
       61 aaattgatcg acagtgaaga aaaagcccgg gcattattga atgtcgtacc cgatgcaatt
      121 gttttactcg aatctaatgg taatatactc gatattaatg aaaaagcagc tgaattgatc
      181 ggtggttcca ccgtaaataa tcaaaaacca aattacttcg accttcttcc cgaacctatc
      241 agaacggccc gtcagaagat tttcaaaaag gttgtaagcg atggaagaag atattctcat
      301 tacgataccc tgttgaaccg acattatgat cttacctttt accctgcatc aaatctcaat
      361 aaaaccatta aaagagttgc tattttcagt agagatatta ctgatatact tgcaaatcaa
      421 gaagctatta ccaaccataa tcatttctta cgtgctcttt tggacaatat ccctaatcct
      481 gtgttctata aagatactaa aggggttttt ctgggatgca acaatgcttt cttacaagcc
      541 ttcggaaaaa cttcaaccga tattatagga aagaagattt gtgaaatttt ttcgatagaa
      601 aattgccgcc aaattatcca aaaagatgag gaaataatta gttcgaagca aatccagagc
      661 tttgaactgg aatttcaata tgctgatgga cttatacaca atataattat caatcaatct
      721 cccttttccc aaagcgatgg tactttaggg ggcttggttg gaatcatgat cgatatcagt
      781 gaaacaaagc agatacagca cgaactcgaa caatggaata tacaattgaa caagagggtt
      841 agcgaagagc ttaagaaagt cgaaaaacag aggcaaatgc ttattcagaa atccaaactc
      901 gaatcattgg gtcagctcgc agctggaatt actcatgaaa tgaatcagcc ccttactgcc
      961 ataaccatgg gactcgaaaa tattcttttc cacttgaaat cagaagacat cacaaagcac
     1021 tatctcgaaa acaaaatagg aaccatctta gagaatattc aacgcatcaa ccgcataatc
     1081 gaccacgtaa aaaccttttc acgcaaccaa caaaatgccc ggtttacccg catgaatctg
     1141 aatgaggcca tacagggagc cttgttgcta ttgaagagtc aattttccga gcgaaatatt
     1201 cattttaatc tgcaacttca tccagaacct ttatattttt tgggcgaccg cttcaaagtg
     1261 gaacaagtcg tcatcaattt gcttaacaat tcgaaagatg ctttggaaca aaaagcccat
     1321 aaccaaagcc ttaattttaa taaaacaatt gccattcgca gctttataat aaactccaat
     1381 aacataggaa tggaaatcga agataatggt acaggcattc cacctcaact gcttgatcat
     1441 atcttcgaac cctttttcac aactaaagaa atcaataaag gtacaggatt aggtttatcc
     1501 atcgtttatg gtattataga ggaattaaag ggaaacatcg aagttaaaag cgaatttggg
     1561 gaaggcacat gctttacctg tgaatttcct tcttacatga ataccaacaa ttgataagcc
     1621 agtcggaatt tataactttg caaaaattcc ttctcataga acattaaaac gaatttgctt
     1681 aagaatggct gagcaactaa atattcttat tgttgacgat gaagagatta ttcgtaacga
     1741 attagccgag tatcttcaaa ataacaattt caatgttttt ttggccgaag cctccaccga
     1801 agccttggag gtattgaagg ccaatcgcgt tgatgttgtt tttctcgata ttcgtttgcc
     1861 aggtataaat gggctcgaac ttttgaaaat cataaagatg gaatatcctg aaaaagaagt
     1921 gattatgatt tcgggccatg gggaaatgca aagcgttatc gaagctatgc gaagcggagc
     1981 atgcgattat ttgcccaaac ctttttcgtt gaccgaagta atttcggcta tcgaacgaac
     2041 gcgccgattt atcgatttga acaaccgact caaagaagtt gaaaattcat tcaatctatt
     2101 taaagacaac attaggaaag aacttggaca aggttttatt gcgcagagtc aggccatgaa
     2161 aaaggtatct gaacttgtaa gtaaagttgc taagagcgat cctaccaatg tgttgatcat
     2221 gggtgaaagc ggcacaggca aagaactagt cgccagagct atacactaca tgagccctcg
     2281 caaggatcat tacttctatg cagtaaactc ttcggctatc accgaaacac tgtttgaatc
     2341 ggaattcttt ggacacacca agggttcgtt tactggcgca aatgaaaata aaaccggatg
     2401 gttcgaagta gccaacaaag gcactttgtt tttagacgaa attggcgatt tgcctctgag
     2461 cctgcagact aaatttctgc gcgtactcga agaacgtaaa atcagcagag taggagggac
     2521 tcaagaaatt ggaatcgacg tcagaatcat tgcagcaacc aatcaagact tcgaaaaact
     2581 taccga
//
LOCUS       DAORPD010000157         2562 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_7240_length_2562_cov_2.600319, whole genome shotgun sequence.
ACCESSION   DAORPD010000157 DAORPD010000000
VERSION     DAORPD010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7240_length_2562_cov_2.600319"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1300
                     /locus_tag="PK976_02610"
     CDS             <1..1300
                     /locus_tag="PK976_02610"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC66811.1"
                     /translation="ATQGSYPVTLTVTKGTESHTTTLENFITAWAEPTAPIAHDTSAC
                     YAMPVPDLIAEGENIRWYSDSLLTNQVYSGNTFPTGITQIGTFNYYVTQSAGTCEGPA
                     TMVTLTISDYPIVTLTPFETVCVNTDPFELTGGNPEGGLWQGIGVDTNYFNPSTAGAG
                     EHPLSYTYTNEFGCSTTISQPITVMDLPEVSLAPFTPLCDNTDPIELTGGVPEGGIYS
                     GNGVENGFFYPSNIGAGETEILYSYTDNTTGCSNFASQSISVKASPIVLFGGDTTICH
                     NHVITLDATNENSTYLWSTGETTPAIIVDSTGVGIGGVKNISVTVNNSDGCIASANIN
                     ITFKDCTGIEELASSLGISIYPNPNQGNFTIEFHAKQPTSVNMRILNLFAQQVFALDN
                     LKINTQTLQKINLDQLPDGIYILVIETNGRTFTQKFVINK"
     gene            1431..>2562
                     /locus_tag="PK976_02615"
     CDS             1431..>2562
                     /locus_tag="PK976_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006949062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRC66812.1"
                     /translation="MKQIFQLSVFVLLATFVFGQQVPREMVILEIGTGTWCTYCPGAA
                     MGADDLLANGCMVAVVENHNGDPFANQYSNARNSFYGITGFPTAIFDGISKVVGGNHS
                     QSMYPTYLPRYNQRIAIPCDFTMDMQITNSGLDYTAVITVTKVAPNTATGLKLHFFVT
                     QSHISYNWQGQNHVNFVNRLMVPDQNGTAIDFSGGDVVIVTLNFSLDPTCPIEDVEFV
                     AGIQAQNKEFLQGTKQAAIDLHVDFTANDTVIPINQPVIFTNNTTGGYIGTPETYQWF
                     FPGATPDTSSLKNPTVTYTECGSHNVKLIVYRGGQIDSLERQAYVQVGPLVNITASPS
                     DTSFWPFNPIVLDATIDDPQATYLWQPGGETTSSITVSFDQYG"
BASE COUNT          750 a          613 c          466 g          733 t
ORIGIN      
        1 tgctactcaa ggctcatatc ctgttactct cacggttaca aagggaaccg aaagtcatac
       61 aactaccctg gaaaatttca tcactgcatg ggcagaacct acagcaccca ttgctcatga
      121 cacttctgcc tgctatgcca tgccagtacc tgatttaatt gccgaaggcg aaaacatacg
      181 ctggtattct gatagtttac ttaccaatca ggtttattcg ggcaacacct tcccaactgg
      241 cattactcaa attgggactt tcaactacta cgtgactcaa tcggcaggta cttgtgaagg
      301 accagctacc atggtaacct taaccattag cgattatccc attgtaacgc tcacaccttt
      361 cgagactgtt tgtgtcaata cagatccatt cgaacttact ggtggaaacc cggaaggcgg
      421 attatggcaa ggcattggag tagatacgaa ttattttaat ccttctacgg caggggccgg
      481 cgaacatcca ctttcataca cttataccaa tgaatttggt tgttctacta ctatatccca
      541 acccattact gttatggatt tacccgaagt ttcacttgct ccttttactc ccttatgcga
      601 taacaccgat cccatcgaat taaccggtgg agttcccgaa ggtggcattt attcgggaaa
      661 tggcgtagaa aatggattct tctatccttc gaatatagga gcaggcgaaa ccgaaatcct
      721 atattcatat accgataata ccacgggttg ttcaaacttt gcttcccagt ccatatccgt
      781 aaaggcatcg ccaatagtct tatttggtgg agatacaact atttgccaca accatgtcat
      841 tacgctggat gctacgaatg aaaactcgac ttatttgtgg tccacaggtg aaactacacc
      901 agcaataatt gtggatagca ctggcgttgg catcggaggt gttaagaata tttcggttac
      961 agtgaataat tccgatggtt gcatagcttc ggcaaatatc aacattacct tcaaggattg
     1021 cacgggtata gaagagctgg cgtcatcctt aggtataagc atttacccca atcccaacca
     1081 gggaaacttc actatagaat ttcatgctaa acaacccacc tccgtgaaca tgagaatact
     1141 caatcttttc gcacagcaag tttttgctct cgataacctc aaaataaaca cacaaactct
     1201 gcaaaagatc aacctcgatc aacttcccga tggaatttac attctggtta tcgaaactaa
     1261 cggtcgaacc ttcactcaaa aatttgttat caacaaataa aagattgaat attcattaaa
     1321 aacttaaccc gcattttatt cacaataaat tttttgaata caatgcgggt ttttattaat
     1381 ttagccctct actttaattg aataccttaa taacctaaaa ctacaaacct atgaaacaaa
     1441 tctttcaact ttcagttttt gtgttgcttg cgactttcgt gttcgggcaa caagttccgc
     1501 gcgaaatggt gatccttgaa ataggcaccg gtacatggtg cacttattgt ccaggcgccg
     1561 cgatgggagc cgacgatctt ctcgcaaatg gctgtatggt agcagttgtc gaaaaccata
     1621 atggcgatcc ttttgctaat caatacagca atgcaagaaa ttccttttat gggattaccg
     1681 gattccctac ggctattttc gatgggataa gtaaagtagt tggtggcaat cacagccaaa
     1741 gcatgtaccc cacttacctt ccccgttaca atcaaagaat tgccataccc tgcgatttta
     1801 ccatggatat gcaaattacc aattcaggac tcgattacac cgcagtaatt accgtgacca
     1861 aagtggcccc caatacagca actggattaa aacttcattt cttcgtaact caatcacata
     1921 tttcatataa ctggcaaggt caaaatcacg taaatttcgt aaaccgcctg atggtgcccg
     1981 atcaaaatgg cactgctatt gatttctcag gtggtgatgt ggttatagtt actcttaatt
     2041 tttccctcga tcctacttgc ccaattgaag atgtagaatt cgttgccggt attcaggccc
     2101 aaaacaaaga attcctccaa ggaactaaac aagccgccat cgaccttcat gtcgatttta
     2161 ctgccaatga caccgttatt cccattaatc aacccgttat ttttacaaat aatacaacgg
     2221 gtggatatat aggtactcct gaaacttatc aatggttctt cccaggagct actcccgata
     2281 cttcctcact gaaaaatcca acggttacct ataccgaatg tggttctcac aatgttaaac
     2341 tcatcgtata ccgtggcgga caaattgatt cattagaacg tcaagcttat gtacaggtag
     2401 gacctctggt caatattact gcctcaccca gcgatacaag tttctggcct ttcaatccta
     2461 tcgtgcttga tgcgacaatc gacgacccac aggctactta tctctggcaa ccagggggtg
     2521 aaaccacgtc ttctattacc gtttctttcg atcaatatgg ct
//
LOCUS       DAORPD010000158         2555 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_7271_length_2555_cov_1.876000, whole genome shotgun sequence.
ACCESSION   DAORPD010000158 DAORPD010000000
VERSION     DAORPD010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7271_length_2555_cov_1.876000"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="PK976_02620"
     CDS             complement(<1..878)
                     /locus_tag="PK976_02620"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRC66813.1"
                     /translation="MKNIRKQGIWALLLLFILAACSPQEDDSYSLGELGTVTSEIISF
                     SQTPSASSNNVITYENTSNLNFPVTAVWALGNGSSARGSKVTASYPEKGDYTVTLTLY
                     AADGSMASKSVVVHIEEDDFGLINTPAYTNLTGGADNLNGKTWVFDQYHDGHFGVGPA
                     DGDSPEWWSCPAQGKEGSSLYTQKFTFFQQGTKMKWENNGYIYTNAAGVQGLGNPAGV
                     IANPGGVGDFDVPFVPNADGYSFKLNEEDMTLELSGGAFFGFYTGTSIFKIYSLTETE
                     LYVKCVSTVEPSNSWW"
     gene            complement(912..2444)
                     /locus_tag="PK976_02625"
     CDS             complement(912..2444)
                     /locus_tag="PK976_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HRC66814.1"
                     /translation="MKKYKFIAIIATLVIGVFSMISCSDDFLSASSTEKPEYGGSATE
                     GSILSNLASAYQILLFDSYANGNYNAVLLMSDLRSDDIYKGGGSAGDQEQLYKLSQFT
                     STAASTLDGLWSIYFTGLARANNVIISCENASGVKEEKLEQYKAEAHFLRAYYVHLLW
                     KFWGNIPYFEEPLSNPPYMAKQLPADSIYNKILKDVDFACVEGRLPMKATGSDLGRAS
                     RAAALMLRARVVLYQKDESRYPQITNDMATIINSGEYELFEDFDAMWRDENEFCKESI
                     FESNQLPEGKTWGNSWSGYGTNLPAFISPADLKDPNSVFKGGWGFGPVRQSAYDMYEE
                     GDVRRDASINKWEDGSYTARFQNTGLFQRKYAARVGYNPPPGDVDLNYCNNLRIFRYA
                     ETLLNYAELIALHGQAEVQGITAQGCLDQIRNRAGVPSITANIENIKLERRREFLGEG
                     MRFWDLIRWGDATTVLTENEPEHNSVRTFEEWMKYLPIPQGEMEKTKGTEFELTQNGN
                     WN"
BASE COUNT          746 a          593 c          455 g          761 t
ORIGIN      
        1 aaccaccatg aatttgaagg ttcaacagtg ctcacgcatt ttacatacaa ttcggtttcg
       61 gtaagcgagt atattttaaa aatgcttgtt ccggtataaa agccaaaaaa tgctcctccg
      121 cttaattcga gtgtcatatc ctcttcattc aatttgaaac tatagccatc tgcattagga
      181 acaaatggca catcgaaatc acctacccca ccaggattcg ctattacacc tgccggattt
      241 cccaatcctt gcaccccggc tgcattggta tagatatatc cattgttctc ccatttcatt
      301 ttcgttcctt gttgaaaaaa agtgaacttc tgcgtgtaaa gcgaagagcc ttcttttcct
      361 tgagccggac acgaccacca ttcaggggaa tctccatcgg ccggacctac cccaaaatga
      421 ccatcatggt attgatcaaa cacccatgtt ttgccgttaa gattatcggc acctcctgtc
      481 aaattggtgt atgccggggt attgataagg ccgaagtcat cttcttcaat gtggacaaca
      541 acgctttttg acgccataga accatcggct gcatacaaag taagtgttac tgtataatcc
      601 cctttttcgg gataagaagc agtaacttta ctacctcttg cagaagagcc gtttcccaag
      661 gcccaaacgg ccgtgacagg aaagtttaaa ttggaagtat tttcataagt aatcacatta
      721 ttgcttgaag cagaaggcgt ctgagaaaaa gatataattt ccgatgtaac tgtccccaat
      781 tctcctaaag aatagctatc atcttcttgt ggactacatg ctgccaaaat aaataataac
      841 aacagcgccc aaattccttg ttttcgtata tttttcatat tgacaaatct ttttaataat
      901 tagaaacaaa attaattcca attcccgttt tgggtcaatt caaattcggt tcctttcgtt
      961 ttctccatct caccttgcgg aatcggcaaa tatttcatcc actcctcaaa agtacgcaca
     1021 gaattatgtt cgggctcatt ttccgtcaaa actgtcgttg catctcccca ccgtatcaaa
     1081 tcccagaaac gcataccttc tcctaaaaat tcacgacggc gttcgagttt aatattttct
     1141 atgtttgccg taattgaagg aactcctgcg cgattacgta tttgatctaa acatccttgg
     1201 gcggtaatgc cttgaacctc ggcctgccca tgtaacgcta taagttcggc gtaattcaat
     1261 aatgtttctg cataacggaa aatacgtaaa ttgttgcaat aatttaaatc gacatctccg
     1321 ggaggcggat tatatccaac gcgtgcggca tatttgcgtt gaaacaaacc ggtattttga
     1381 aaacgagcgg tataggatcc atcttcccat ttgttaattg aagcatcacg acgtacatct
     1441 ccttcttcat acatatcata agcactctga cgcacagggc caaagcccca acctcctttg
     1501 aatacagaat taggatcttt gagatcggct ggcgaaataa acgcaggcag gttcgtgcca
     1561 taaccgctcc atgaatttcc ccatgttttt ccttcaggaa gctggttgct ttcgaaaatg
     1621 gattctttac aaaattcgtt ctcgtctctc cacatggcat caaaatcttc gaagagttca
     1681 tattctccgc tattgataat tgtggccata tcattggtga tttgcggata tcgagattcg
     1741 tctttttggt aaagaacaac cctcgccctc aacattaatg cggcagccct tgaagcacgt
     1801 cctagatcac tacctgttgc tttcattgga aggcgccctt caacacaggc aaaatccaca
     1861 tctttcaaaa ttttattgta aattgagtca gccggtaatt gcttggccat atacggagga
     1921 ttggaaagtg gttcttcgaa atatggaata ttaccccaaa atttccaaag caaatgcaca
     1981 taatatgctc tcagaaaatg agcctcagct ttatattgtt ccagtttttc ctctttaaca
     2041 ccagaagcat tttcacagga tatgataaca ttgttagctc gagctaaacc cgtaaaatag
     2101 atactccaga gtccatccag tgtcgaagca gcggtggatg taaattgcga cagtttatac
     2161 aattgttcct gatcaccggc actacctcca cctttataaa tatcatctga ccgcagatcc
     2221 gacattaata aaaccgcatt gtaattgcca tttgcataac tgtcgaacaa aagaatttga
     2281 tatgcagacg caagatttga caggatcgat ccttccgtag ccgaacctcc gtactcgggt
     2341 ttttcggtcg acgatgcact taaaaaatcg tcactacagg aaatcataga gaaaactcct
     2401 atcacgagag tagctatgat tgcaataaat ttatattttt tcattttttc tatatattta
     2461 gaattaatag gttagaatga cacattcgct ccaagataaa ttgttcttga ttgaggataa
     2521 acacctctat cgactccgat acgggtatac tcgcc
//
LOCUS       DAORPD010000159         2554 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_7274_length_2554_cov_2.695478, whole genome shotgun sequence.
ACCESSION   DAORPD010000159 DAORPD010000000
VERSION     DAORPD010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7274_length_2554_cov_2.695478"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="PK976_02630"
     CDS             complement(<1..629)
                     /locus_tag="PK976_02630"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HRC66815.1"
                     /translation="MNTPRFEIYRASAGSGKTFLLSIHFLSLVLEDPEVFRRILGLTF
                     TNKAAKELKERILYFLGLLAKLPNCNDGKLKVSILENLKNEGWNSSDEQTQQKARKAL
                     ENILHYYGYFSISTIDAFMYRVVNSFTFDMKIAHDFEVELNEDVLIDEAIQRLLDRVG
                     REKVITELLQNILLEMVDDERGWNIEREIREMAEIIFLEKSNEPLSELGT"
     gene            721..1914
                     /locus_tag="PK976_02635"
     CDS             721..1914
                     /locus_tag="PK976_02635"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HRC66816.1"
                     /translation="MSKFATIMVALEEAIEKVMNEARVLPMEEISLTEAQGLVLAEDV
                     FSDLDMPPFRKAAVDGFACRREDLDKPLKVIETIAAGELPQKSIGVQECTRIMTGAMV
                     PEGADCVVMIEHTIGEDEGTIRFIGDKTSDNIAQKGEDIRKGEKVLSAGTVLEAQHIA
                     VLASVGRFTPKVYRRPLVGIIATGDELVEPWEQPKAGQIRNSNAWQTIAQVKKTGAEY
                     IYDGIVKDNPQALSEKINLFRQKVDVLLFSGGISMGEYDYVPQVMKDAGLSLIFKDID
                     IQPGRPTLFGILDHIAVFGLPGNPVSSFVQFELMVHPFIYTSMGKTFHAHIRHLPIGE
                     DFYRTKANRRSLIPVRIENDWIFPVKYHGSAHINAYTFASGWIDIPKGKKELKKGEMI
                     DVRLL"
     gene            1898..2386
                     /locus_tag="PK976_02640"
     CDS             1898..2386
                     /locus_tag="PK976_02640"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRC66817.1"
                     /translation="MLDSYRREINYLRISVTDRCNLRCRYCMPSEGFRWIPHENILRF
                     EEIIEIVREAVKLGIHKIRVTGGEPLLRKGIVNLIEMLASIEGVQDLSMTTNGTHLEK
                     FARELKQAGLQRINISLDTVNAERYAYLTGGGNIERVLRGIDAALEAELAELRKQLDQ
                     SR"
BASE COUNT          760 a          556 c          565 g          673 t
ORIGIN      
        1 gttcccaatt cactcaaggg ttcattcgat ttctcgagaa agatgatttc ggccatttct
       61 cggatttctc tttcgatgtt ccagcccctt tcatcatcca ccatttcgag gagaatattt
      121 tgcagtaatt ccgtaataac tttttctctc ccaactcgat cgagcaagcg ttgaatggcc
      181 tcatcgatca atacatcctc gttaagttct acctcgaaat catgtgcaat tttcatgtcg
      241 aaagtaaatg aatttaccac tctatacatg aatgcatcta tggtggaaat ggagaaatag
      301 ccataataat gtaaaatatt ttctaaggcc ttcctggcct tttgttgggt ttgttcatct
      361 gaactgttcc atccctcatt ttttagattt tccaaaatgc tgactttgag cttgccatca
      421 ttacaatttg gcaacttagc cagcaaaccc agaaaataca atatgcgttc ctttaattct
      481 ttagccgcct tattggtaaa tgttaagccg agaattcgac gaaaaacttc gggatcttcc
      541 aaaacaagcg agagaaaatg tatgctcagc aaaaaggtct tacccgaacc ggccgaggcg
      601 cgataaattt cgaatctggg tgtattcatg agtcaaaagt aaggagaatt gttcatgaaa
      661 cttagtgaga atacgaaaga atgaaaaatt ttcgagatga acgtatcgca accattttca
      721 atgagtaaat ttgcgacgat tatggtagct ctcgaagaag cgatagaaaa agtgatgaat
      781 gaagccaggg tgcttccaat ggaagaaatc tcccttaccg aggcgcaggg tctcgtactg
      841 gccgaagatg tattttcaga cctcgatatg ccgcctttca gaaaagcagc cgtagatggg
      901 tttgcatgcc gaagagagga tttggataaa cccttgaagg tgatcgagac catagctgca
      961 ggagaactgc cacaaaaatc catcggagtt caagaatgta cgcgaattat gacgggagcc
     1021 atggttcccg aaggagccga ttgcgtcgtc atgatcgaac ataccattgg agaagatgag
     1081 ggtacgattc gcttcattgg cgataaaacc tccgacaaca tcgcccaaaa aggagaagac
     1141 attcgaaagg gagaaaaagt attgagtgct ggaacagttc tcgaggccca gcatattgcc
     1201 gtactggctt cggtgggtag atttactcct aaggtgtata gaagaccact ggttggcata
     1261 attgctactg gcgacgagtt agttgaacca tgggaacaac cgaaagccgg tcagattaga
     1321 aatagcaatg catggcaaac catcgcacag gtaaagaaaa ccggagcaga atatatttat
     1381 gacggcatag tcaaggataa tccacaggca ctaagtgaaa aaatcaatct gttcaggcag
     1441 aaagtcgatg tgctcctgtt ttcgggtgga atctctatgg gtgagtacga ttacgttcct
     1501 caggtgatga aagatgccgg attgagtctt attttcaaag atatcgacat acaacctggt
     1561 cgtcctactc tctttggcat attagaccat atcgccgtat ttggcttacc gggaaatccg
     1621 gtttccagct tcgtgcagtt cgaacttatg gttcatcctt tcatttatac ctccatgggt
     1681 aagacctttc atgcccatat caggcattta ccaataggag aagactttta cagaaccaaa
     1741 gccaatcgtc gctcattgat tcccgtgagg atagaaaatg attggatttt cccggtgaaa
     1801 tatcatggct cggctcatat caatgcatac acatttgctt caggatggat cgatatccct
     1861 aaagggaaaa aagaattgaa aaaaggagaa atgatagatg ttagactctt atagacgcga
     1921 aattaattat ttacgtattt ctgttaccga tcgatgcaac cttcgttgtc gttattgcat
     1981 gccttcggaa ggttttcgtt ggattccaca cgaaaatatc ctaagattcg aagaaattat
     2041 agaaattgtg cgagaagctg taaaactggg tattcacaag ataagagtga ccggtggaga
     2101 gcctttactt cgaaagggaa ttgtaaacct aatagagatg ttagccagca tagaaggggt
     2161 acaagacctt tccatgacga ccaatggaac tcatctggaa aaatttgccc gagaattaaa
     2221 acaagctgga ttgcagcgta tcaatatcag cctcgatact gtcaatgctg aacgttatgc
     2281 atatctcaca ggaggaggca atatcgaaag ggttcttcgc ggcatagatg ccgccctcga
     2341 agctgagctc gccgaactgc gcaagcagct tgaccagtca aggtagctgc acaccgcaaa
     2401 cttcctccgt tccgctaaac tgcgtggtac aatcgcacca tcgacacacc caccgtgcca
     2461 cattgaacca aggccaaagg tcatgccatc tatgcggatc cgccccgccc tcattctatc
     2521 tctcatcctg ctcgccctgc tgccgcgtcc atct
//
LOCUS       DAORPD010000160         2538 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_7334_length_2538_cov_2.276279, whole genome shotgun sequence.
ACCESSION   DAORPD010000160 DAORPD010000000
VERSION     DAORPD010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7334_length_2538_cov_2.276279"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="PK976_02645"
     CDS             <1..361
                     /locus_tag="PK976_02645"
                     /inference="COORDINATES: protein motif:HMM:NF024790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HRC66818.1"
                     /translation="FTSMSLFCLVFFVRNIYGVIWLLAFTVLNMAALYFLSYEMIYWI
                     AFVFSVLLLGDALASVLFLLWLSIRTPRKSGDAHSLRQLTGIHEAVWALIFVAFALWI
                     TYDIIIKFFPAFPSLKV"
     gene            complement(775..1794)
                     /locus_tag="PK976_02650"
     CDS             complement(775..1794)
                     /locus_tag="PK976_02650"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRC66819.1"
                     /translation="MNSSKSLAGIFLSIIILFSISCTQKKEKASDNQPDTIPMALKSL
                     NERLKKNPQNIESYYERANWFIQNARYGEALKDLHAVMKKDSLNPKLYSLLGDVYLNL
                     GKPIQAEEYYSKALHRDPTYNDALVGLARNYLVLKDYDQTFALTAKAITHNALNPKAY
                     YLAAWAFAEKGDTLSAIKNYLKAIEQDQDFFDAYVQLGILYGKKQPQMAEGFFKNALR
                     IKPNHIHTMYLLALLYQNTGQTDKALSAHQSVLAIDPNYSPSMFALGYIYMADILDFQ
                     TALTWFDRVIQLDEKNAAAYLNRGYCYEQLGIKNKASSDYHRALNLQPNFQKAVEALQ
                     RIGEK"
BASE COUNT          705 a          467 c          530 g          836 t
ORIGIN      
        1 ttttacatcc atgagtctgt tttgtttggt attttttgtc aggaatattt atggagtaat
       61 ctggttactc gcgtttactg ttcttaatat ggcggcctta tattttctgt cgtatgagat
      121 gatttactgg attgcattcg tttttagtgt acttttgttg ggtgatgcct tggcctcagt
      181 gttgtttctc ctctggctca gtattcgaac gcctcgtaaa agcggcgatg cccacagcct
      241 gaggcagctt acgggaattc acgaggcagt atgggctttg atctttgtgg cattcgcctt
      301 gtggataact tacgacatca ttatcaaatt ttttcctgct tttccttcat tgaaagtatg
      361 atacagaaaa ttcttacctt catcatagtt atttcggcag tcttttgggt ggtttatcgt
      421 ttctatagat tttctaaaca aaatgtgggc ccctgtggag gttgcatttc aaaaaattgc
      481 agctcatgcc cagccattga tttgaaagca gaagccgaac gccgaaagaa cgccaaatcg
      541 gaaaaatctg cagaacgata aaatcgatct tgccagaaat gcgcatagca tcaagggaat
      601 tatgtcaatt tttatgatga aaatgctcgc tgcttggctt ttcggctcga gaaaaaagga
      661 agatcatcga atggttctgg cttagatgaa gatttatttc tattggagaa agtctgtgag
      721 catttaatcg ccgaactgac cgaggtacgt gagagttatc tctaattctt aaggttattt
      781 ttcaccaatt cgttgaaggg cttctactgc cttttgaaag ttgggttgaa ggtttagagc
      841 cctgtgataa tcggacgaag ctttgttttt aatgccaagt tgttcatagc aatatccacg
      901 gtttagatat gctgcagcat ttttctcgtc gagttgaatc acgcggtcga accacgtaag
      961 tgccgtttga aaatcgagta tatcagccat ataaatataa ccaagggcaa acatggaagg
     1021 agaatagtta ggatcgatgg ccagaaccga ttgatgggca gatagagcct tatcggtttg
     1081 tcctgtattt tgataaagca gtgcgagtaa atacatcgta tgaatatggt tgggtttgat
     1141 gcgcagggca tttttaaaaa atccctcagc catttggggt tgttttttcc cataaagaat
     1201 tcctaattgc acataggcat caaaaaaatc ctgatcttgt tctatggctt tcaaataatt
     1261 ttttatggcg ctcaatgtat ctcctttttc ggcaaacgcc caggctgcca gatagtaagc
     1321 ctttggattc aaggcattat gcgtaatagc cttagcagta agggcgaatg tttgatcgta
     1381 atctttcagt accagatagt ttctggctaa tcctaccaaa gcatcattgt atgtaggatc
     1441 gcggtgaaga gccttagaat aatattcttc agcctgaatg ggtttcccaa ggttaagata
     1501 cacatcaccc aacaaactgt atagttttgg attcagagaa tcttttttca tcactgcatg
     1561 gaggtcttta agagcttcgc catatcttgc attttgtatg aaccagttag ctctttcgta
     1621 atatgattct atattttgtg gatttttctt caagcgttca tttaaacttt tcaaagccat
     1681 agggattgta tcgggctggt tatccgatgc tttctccttt ttttgtgtac atgaaattga
     1741 aaaaaggatt attatggaaa gaaaaattcc tgcaaggctt ttcgacgaat tcataaaggc
     1801 agaatgtgat ttgaaccagc aaaattagag tttttgtaac aggtaatgaa aacatcattt
     1861 ggttattatt caatcaatga tgagatttga aggtagggag attttgagac ttggggactg
     1921 ggagacgagg agaggggagg gggagatggt gagacgggga gacttggggg ccgccaaagt
     1981 cgaaagaact atattttttc tcgctgattg acgcagattt atatcgctga ttcatgttga
     2041 tatttttctg cgagcatctg cgaaggcttg tttctgcgat atctgcgata acatttttat
     2101 ttttctcgct gatccaccct cttttaacaa ctaacaacca ccaattccca aattacaatc
     2161 aaatttcaaa ttctaatcaa caatcactaa tcaccatcca ccaatccacc ttgcttccca
     2221 agtaccatct ccaaccacca acccccatca ccattctcta ttctcctttc ctccgttctc
     2281 ccttccttat attattaaaa tctctctttt tttgaaattt cttttacatt attagtgtct
     2341 tagcaagaaa aaattagcac gtagagatca aaggccgcaa aaatttgaac ttgctttctt
     2401 ggtgttgtta gcgttttgga gtggaaatta aaggatgcga agaccaaaaa atagcaaaga
     2461 atttgattag cgtttctaaa ttagaacttt aaataatatt tgtcggcaat tagagactca
     2521 aaaggtcaga gtttggcg
//
LOCUS       DAORPD010000161         2537 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_7342_length_2537_cov_2.406930, whole genome shotgun sequence.
ACCESSION   DAORPD010000161 DAORPD010000000
VERSION     DAORPD010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7342_length_2537_cov_2.406930"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(171..620)
                     /gene="rplI"
                     /locus_tag="PK976_02655"
     CDS             complement(171..620)
                     /gene="rplI"
                     /locus_tag="PK976_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HRC66820.1"
                     /translation="MEVILIQDVPKLGYTDEIVNVKNGYALNYLIPKGYAIMANPSNR
                     KVLAENLKQKAFKQEKVQNEALELAKKLDGLLIRVGAKASSSGKIYGSVNALQIAEVL
                     KNQHHIEVDRKRILIDGDSIKELGTYTATINLHKDVKATIQFEVFAE"
     gene            complement(634..903)
                     /gene="rpsR"
                     /locus_tag="PK976_02660"
     CDS             complement(634..903)
                     /gene="rpsR"
                     /locus_tag="PK976_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HRC66821.1"
                     /translation="MAQGESEIKYLTPIAVETKKKKYCRFKKSGIKYIDYKDESFLLK
                     FVNEQGKILPRRLTGTSLKYQRKVAQAIKRARHLALLPYVADLLK"
     gene            complement(923..1276)
                     /gene="rpsF"
                     /locus_tag="PK976_02665"
     CDS             complement(923..1276)
                     /gene="rpsF"
                     /locus_tag="PK976_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006254256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HRC66822.1"
                     /translation="MNNQYETVFIMTPVLSEDQMRETVKKFHDLLVEKGAEIVHEENW
                     GLRKLAYPIQKKSSGFYQLFEFKAEGSLINELETAYRRDERIIRFLTVLLDKHAIAYN
                     EKKRTNKNDIPTLND"
     gene            complement(1413..>2537)
                     /gene="wecB"
                     /locus_tag="PK976_02670"
     CDS             complement(1413..>2537)
                     /gene="wecB"
                     /locus_tag="PK976_02670"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016662694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HRC66823.1"
                     /translation="IGARPQIIKAAAISRTIRDFFTETIQEIIVHTGQHYDENMSQVF
                     IEQLQIPTPHYNLGIGSASHGIQTARMIEKIENVLLTEKPDFVILYGDTNSTLAGAVA
                     GAKIHIPVAHVEAGLRSFNKLMPEEINRIVCDHCSTLLFSPTATGFHNLVREGFLPDT
                     LPPYHINNPKIYHCGDVMYDNCLYFSDMAERNSQILQQYGLNPGKFILITIHRDSNTD
                     QPQRLESIFRSLIRLTENTHLPVVLPIHPRTSKVLKSKLQLDPYKNLQHQPNILIIPP
                     ASFFDMILLEKNAALILTDSGGVQKEAYFYRKPCVILRAETEWKEIIETGAGALADAN
                     EEQILTKSIEYLKNPPRNFPPIFGDGLTAHFILNEIVATE"
BASE COUNT          710 a          464 c          529 g          834 t
ORIGIN      
        1 gaagcaaatc agtaactaat ttttctcctt ctgccaggaa aactccttcc tgatagcgaa
       61 aggatttctc cttgagtttt ctaatgaaag ctatttgttg atgtgaaagc atagataaaa
      121 aacccgccat ggagggtcat gacgggacca attattgaaa gccaaacaaa ttattctgca
      181 aagacttcaa attgaatagt agctttgacg tctttatgaa gatttatggt tgccgtgtat
      241 gttccgagtt ctttaatgga atccccatcg atgaggatgc gtttgcgatc tacttcgata
      301 tgatgttgat ttttaagaac ttcggcaatt tgcaatgcat tcaccgaacc ataaatttta
      361 ccgctgctcg aagctttggc acccactctg atgagaaggc catctaattt tttagccaac
      421 tcgagggctt cgttttgaac cttttcctgt ttaaaggctt tttgtttaag attttcggcc
      481 aatactttac gattagaagg attcgccata atagcgtatc ctttggggat gagataatta
      541 agtgcataac cgtttttcac gtttacgatt tcgtcggtat agcccagctt aggtacgtct
      601 tgaatcaaaa taacttccat acttttttcc tccttatttg agtaaatcag ctacataggg
      661 aagaagtgca agatgacggg ctcttttgat ggcctgagcc acttttcttt ggtatttcaa
      721 ggaagtaccc gtgagcctgc gcggcaaaat ctttccctgt tcgttgacaa atttcagcaa
      781 aaatgactcg tctttgtaat caatatattt tataccgctt tttttgaaac ggcagtattt
      841 cttctttttg gtctcaaccg ctattggagt caaatatttg atttcacttt caccttgtgc
      901 cataatgttc aatttttagt gttcaatcgt ttaatgtagg aatgtcgttt ttgttggtac
      961 gctttttttc attgtaagca atagcgtgtt tatcgagcaa taccgtgaga aaacgtataa
     1021 tacgttcatc acgtcggtag gcggtttcca attcattaat caggctgcct tcggccttaa
     1081 attcgaagag ctgataaaaa cccgatgatt ttttttgaat tggataggca agcttgcgca
     1141 aaccccagtt ttcttcgtgc acaatttcag cacctttttc caccagtaaa tcgtggaact
     1201 tctttaccgt ttccctcatc tgatcttcag acaagacggg agtcataatg aaaacggttt
     1261 cgtactggtt attcatttaa ttgaaattaa attaattaga ctgaataaat tttttattaa
     1321 attggatggc aaaattatta aattcttaca aattgcacaa aaaaaatcgt aaaatttagc
     1381 taaagttcag cattcaggat ggatttagga gcttattccg tagcaacaat ctcatttaaa
     1441 ataaaatgtg cagtcaggcc gtcgccaaaa atgggaggaa aattccgtgg aggattcttc
     1501 aaatattcta tgctcttggt taagatttgt tcttcgttgg catcagccaa agcccccgca
     1561 cctgtttcta taatttcttt ccattcggtt tcggctcgaa gtattacaca aggttttcga
     1621 taaaaatagg cttctttttg tactcctccc gaatcggtca aaataagagc cgcattcttt
     1681 tcgagtaaaa tcatatcgaa aaaggaagca ggtggaataa taaggatatt gggttgatgc
     1741 tgaagatttt tatagggatc gagttgaagt ttcgatttca agactttgga tgttcgggga
     1801 tggataggca aaacaacagg caaatgggtg ttttctgtca agcggatgag cgatctgaat
     1861 atggattcga gcctctgagg ctgatcggta ttgctatcgc gatgaatagt gattagaata
     1921 aattttcctg gattaagccc atattgttgt aaaatttggc tgttcctttc ggccatatca
     1981 gagaaataca ggcaattatc atacatgaca tcaccacaat gataaatttt aggattgttg
     2041 atgtggtaag gaggtaaagt atcgggcaga aatccttcgc gtacgagatt atggaaaccc
     2101 gtggctgtgg ggctgaaaag caaggtgctg caatgatcgc aaacaatgcg attgatttct
     2161 tcgggcatga gtttgttgaa tgatctcaat ccggcttcaa catgagcgac ggggatatgg
     2221 atttttgctc cagctacggc tcctgcaaga gtcgaattgg tatctccata caaaatgaca
     2281 aaatcgggtt tttcggtaag aagcacgttt tcgatttttt ctatcattcg ggcagtttga
     2341 atgccatgcg aagcagaacc tattcctaaa ttgtaatgtg gggttggaat ttgaagctgc
     2401 tctataaaaa cctgcgacat gttttcgtcg tagtgctggc cagtgtggac aatgatttct
     2461 tgaatggttt ccgtaaaaaa atctcttata gttcttgaaa tagccgcagc ttttattatt
     2521 tgtggccttg ctccaat
//
LOCUS       DAORPD010000162         2531 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_7367_length_2531_cov_2.180129, whole genome shotgun sequence.
ACCESSION   DAORPD010000162 DAORPD010000000
VERSION     DAORPD010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7367_length_2531_cov_2.180129"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            162..1163
                     /locus_tag="PK976_02675"
     CDS             162..1163
                     /locus_tag="PK976_02675"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HRC66824.1"
                     /translation="MTKIRAAITGVGAYVPDYILTNEEISRMVDTSDEWIMTRVGIKT
                     RRILRGKGKGTSDLGTPAVNELLRKTGTKPEEIDLLICSTVTPDHLFPSTSAIIADKV
                     GIKNAFCFDLNAACSSFLIGLSSAAKFVESGRYKKVVLVGADKMSSITDYTDRSTCPL
                     FGDAAGAVLIEPTADTEVGYIDEILHTDGYGRIYLYQKAGGSVYPPTHRTVEAREHYI
                     YQEGQVVFKAAVASMADVAVEMMEKHGLTSETIHWLVPHQANNRIIEAVARRMRLPKK
                     KVMINIDKYGNTTSATLPLCLYEWEHQMHKGDNIILATFGAGFTWGAIYLKWAYDPR"
     gene            1279..2079
                     /locus_tag="PK976_02680"
     CDS             1279..2079
                     /locus_tag="PK976_02680"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HRC66825.1"
                     /translation="MKKLYLAFISCLFIVVLNSCSKDDTSINIFTIEDDIALGEQVSN
                     EIAANPQEYPILDPVKYASAYDHLYRIRNTILASGKVKYATKFRWNCKIIRNDTMINA
                     FSLPGGDMYFYTGIIKFLDNEAQFAGVMAHEMAHADRRHSTDQLTKVYGLEILLGVLL
                     GSNPNFAEQIAAELALGLGTLAFSRSQEYEADEYAVKFLYATEYDAASLGDFFEKMEG
                     EPYPPTFLSTHPSPEDRLAKIEEHFQALGGMHGATFAERYKEFKNALP"
     gene            complement(2083..>2531)
                     /locus_tag="PK976_02685"
     CDS             complement(2083..>2531)
                     /locus_tag="PK976_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66826.1"
                     /translation="WDKGLEEVMEGDVKWGVNLYYRQIQEMIEREKTDIVGHLDKIMM
                     YNAHRYFSENEPWYRYLVIETLMKVQQCGAILEINTRGLYKKLSDQLFPSETFLPVIK
                     ELGIPVVISADAHHPSEIGLLFSQTLDLLLSKGFEEINHKGFKCYN"
BASE COUNT          707 a          498 c          560 g          766 t
ORIGIN      
        1 tggaagtcga aaagatgaaa taaatttctt catacttaaa atttgtattg attgaaagat
       61 tctttgacaa gagatttttg tgagaatttt ttttccttat tgcctgaggt taaatcgttt
      121 cgaagcagaa tacattacaa tctattttaa ttgcaattaa aatgaccaag atacgtgctg
      181 caattacggg agtaggtgct tatgtaccgg attatatcct gaccaatgag gagattagca
      241 gaatggttga tacctcggat gaatggatca tgacgcgggt tggaatcaag actcgaagga
      301 ttttgagagg gaaaggcaaa ggtacatccg atttgggaac tcctgcggtg aatgaacttt
      361 tacgcaaaac aggaacaaag cccgaagaga ttgatttatt gatttgttcg acggtaaccc
      421 ctgatcatct ttttccttct acttcggcaa tcatagccga taaggttggg attaagaatg
      481 ccttttgttt tgatttgaac gcagcctgtt cgagttttct tattggtctt tcgagtgctg
      541 cgaagtttgt agaatcgggg cgatataaga aggtagtgtt ggtaggtgcc gataagatgt
      601 catccattac ggattatacc gatcgtagca cttgcccttt gtttggagac gctgctggtg
      661 cggtgcttat cgaacccacc gctgataccg aagttggcta tatcgacgaa atacttcata
      721 ccgatggata tggccgcatc tatctgtatc aaaaagccgg aggttcggta tatcctccta
      781 cccatcgtac ggttgaagcc cgtgaacatt acatctatca ggaagggcaa gtcgtattta
      841 aggccgcagt agcctctatg gccgacgttg ccgtcgaaat gatggaaaaa catggattaa
      901 cttctgaaac tattcattgg ttggttcccc atcaggccaa taaccgtatc atcgaagcag
      961 tagcccgacg tatgcgcctg ccgaaaaaaa aggtgatgat aaatatcgat aaatatggta
     1021 ataccacttc ggccacactt cctctttgtt tgtacgaatg ggaacatcag atgcataaag
     1081 gagataatat cattctggct acttttggtg ctggcttcac ctggggcgct atctatctga
     1141 aatgggccta cgatcccaga taaaccaagg taatcataat tgcctttcat gaggagaata
     1201 tttctgttta ttgcgtgtga gttgattttc ttttagtaat tttgtttaga taacctcctc
     1261 agaaatcctc ccgaaactat gaaaaaactc tatctggcat ttatctcttg tttgtttatc
     1321 gtcgttttga atagttgttc gaaagacgac acttccatca atatttttac catcgaggat
     1381 gatatagcct taggtgagca ggtttccaat gagatagccg caaaccctca ggaatatccc
     1441 attctcgatc ctgtcaagta tgcttcggct tatgatcatc tttatcggat tcgcaatacg
     1501 attcttgctt caggaaaggt aaaatatgcc acaaagtttc gatggaactg caagatcatc
     1561 aggaacgata ctatgattaa tgcgtttagc ctgcctggag gcgacatgta tttctatacg
     1621 ggtatcatca agtttttgga taatgaggcg cagtttgcgg gagtaatggc tcatgaaatg
     1681 gcccatgccg acaggcgtca ttctactgat cagctcacaa aagtctatgg tttggagatt
     1741 ttattaggag ttttacttgg tagcaaccca aactttgccg aacaaattgc tgctgagtta
     1801 gctttaggcc ttggcacgct tgcctttagc cgttctcagg aatacgaagc cgacgaatat
     1861 gcggtaaagt ttttatatgc cactgaatat gatgcggcca gtttgggaga tttcttcgaa
     1921 aaaatggaag gcgagccata tccacctaca ttcttaagta cacatccttc tccagaagac
     1981 aggctggcga agatcgaaga gcattttcag gctctgggtg gaatgcatgg cgccactttc
     2041 gcagaacgtt acaaggaatt taaaaatgct ctcccttaaa atttaattat agcacttgaa
     2101 ccctttatgg tttatttctt cgaaaccttt cgagaggaga agatcgaggg tttgcgaaaa
     2161 taataatcca atctcggatg gatgatgagc atcggccgat ataaccacag ggatgcctaa
     2221 ttcttttatg acaggaagaa atgtttcgga cgggaatagt tgatcggaca gtttcttata
     2281 gagtccgcgc gtatttattt cgaggatagc cccgcattgt tggaccttca tgagagtttc
     2341 gatgacaaga taacgatacc agggttcatt ttccgagaaa taacgatgag cattatacat
     2401 cattatttta tcgagatgac ctacaatatc ggttttttct cgttctatca tttcctgtat
     2461 ttgtcgatag taaagattca caccccattt aacatcgcct tccattactt cctcgagacc
     2521 cttgtcccat a
//
LOCUS       DAORPD010000163         2525 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_7398_length_2525_cov_1.914980, whole genome shotgun sequence.
ACCESSION   DAORPD010000163 DAORPD010000000
VERSION     DAORPD010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7398_length_2525_cov_1.914980"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1081)
                     /locus_tag="PK976_02690"
     CDS             complement(<1..1081)
                     /locus_tag="PK976_02690"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRC66827.1"
                     /translation="MSSCHEQKQEADLIVIHANVYTLDEHFSQQEAFAIKEGRILSTG
                     KTKEILKKFSARDTLDLHGKTVYPGFMDAHCHFLGLGETMIRWANLKGTRSMDEIIER
                     LKSFIAENPQTWLLGRGWDQNLFPDNKFPDNSLLEKNFPNIPVVIVRVDGHAALANDM
                     ALRLACISSDTKIDGGEILISQGKPTGLLLDNAKEKLMEAIPAMDEQLLKHALQKAEA
                     ICISYGLTAVNDAGLPTHVIHTIDELQKNGVLKIRIVAMINPDSLTISEFMHHGIYKT
                     DRLLVNAIKLYADGALGSRGALLKSPYSDDPNTHGLLVESPQILKEICLKAYNSDFQV
                     CTHAIGDSANRLMLDIYSEILKGKND"
     gene            complement(1142..1807)
                     /locus_tag="PK976_02695"
     CDS             complement(1142..1807)
                     /locus_tag="PK976_02695"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRC66828.1"
                     /translation="MDGDDNPATGCRVNGTNAEFLREFSLHDGLHRNFSAIIVGDNHN
                     IAACSIQTDLPYPDTLILIPVHLSCCSLDKERIAETQAIMYYLEKNYSVWRKEDTILR
                     LPVVITGDFNMVGSEIPYYNIITIQLTQAQKPWFTDLAPHQIDRPLAFTWRDYSKAFS
                     PNRLDFIFYSPLLNAVYSFVFHLLYLNENWLEKYGLNPLDLHFCSDHLQLVSDFALAA
                     SPN"
     gene            complement(1890..>2525)
                     /locus_tag="PK976_02700"
     CDS             complement(1890..>2525)
                     /locus_tag="PK976_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRC66829.1"
                     /translation="VVHDVLQMVGIHCKAAEFFDHLSDGEKQKVMIAKALAQQTPYIF
                     LDEPAAFLDFPSRIEIMQLLANISHQQNKTILISTHDVPLAFRQADEIILMATDKPIV
                     SGVPNEIFASSLLEDYFEHNGKKFNPFSFDYGVEQTQGLPVFIDVNEELHPLIAAFIK
                     RRGFLPVAKAENSQHWLRIDANWEIRFSLYQQVYTFHNLEELDLFLKNAKK"
BASE COUNT          766 a          495 c          540 g          724 t
ORIGIN      
        1 gatcattctt acctttcaat atttcggaat aaatatccag catcaatcgg ttggccgaat
       61 cgccaatggc atgtgtacac acctgaaaat cagaattata tgctttaaga cagatttctt
      121 tcagtatttg gggagattca acaagaagtc catgagtatt ggggtcatcg gaataaggac
      181 tttttaaaag agcacctcga ctgcccaaag ctccatcggc ataaagtttt atagcgttta
      241 ccaacaaacg atcggttttg tatatgccat gatgcataaa ttcagaaatt gtgagcgaat
      301 caggattgat catggcaaca atcctgatct ttagaactcc atttttttgt aattcatcta
      361 tcgtatgaat gacatgagtc ggcagtcctg cgtcatttac cgcagtcaat ccatacgaaa
      421 tgcaaatagc ttcagccttt tgcagggcat gtttcagcaa ttgttcatcc atggcgggga
      481 tggcttccat caatttttcc ttggcattat ccagaagcaa tccagtgggt tttccctgag
      541 aaattaaaat ttctccaccg tctattttag tatcggaaga tatacaggcg agtcgaagtg
      601 ccatgtcgtt ggccagagct gcatgaccat ccacccgaac tataactact gggatattgg
      661 gaaaattttt ctcaagtaag gaattatcgg gaaacttatt atcagggaag agattttggt
      721 cccatcccct acccagcaac catgtttgag gattttctgc tataaacgac ttcaagcgtt
      781 cgatgatttc atccatggaa cgagtgcctt ttaagttagc ccatcttatc atggtttcac
      841 ccaatcccaa aaaatggcag tgtgcatcca taaagcctgg gtaaacggtt ttcccgtgta
      901 aatcgagtgt atcacgggcc gaaaacttct tcagaatctc tttggttttg cctgttgata
      961 gaattcgccc ttccttgatg gcaaaggcct cctgttggct gaaatgttca tcgagtgtat
     1021 aaacattcgc atgaatgaca atgaggtcgg cttcctgttt ttgctcgtgg caactactca
     1081 atactagaaa tatcatagga aaaatagata aagaaaagat ttttttcata gacaaaagtt
     1141 attaattggg tgaagctgcc aaggcgaaat cgctgaccaa ttgaagatga tcggaacaaa
     1201 aatgcaaatc cagcggattc aaaccatact tttcaagcca attttcattc agataaagaa
     1261 ggtgaaagac aaaggaataa accgcattga gtaaagggga gtaaaaaata aaatcgaggc
     1321 ggttaggact gaatgccttg gaataatcac gccaagtaaa ggctaaaggt cggtcgattt
     1381 gatggggtgc cagatcggta aaccaaggct tttgagcttg ggtgagctgg atagtaatga
     1441 tgttgtagta aggaatttca gagcccacca tattaaaatc tcctgttatc acgactggca
     1501 gccgcaatat cgtatcttcc tttctccaaa cagaataatt tttttctaaa taatacatga
     1561 tggcttgagt ttcggctata cgttccttgt ctaatgaaca acaggaaaga tgaacaggaa
     1621 tcaaaataag tgtatccgga taaggcaaat ctgtttggat ggaacaggcg gcaatattat
     1681 ggttgtcgcc aacgataatt gcagagaaat ttctatgaag gccatcatgc aagctaaatt
     1741 cccttaagaa ctcagcgttt gttccattca cacggcagcc tgtggctgga ttatcatcgc
     1801 catccataat aatcgggttt ctataacttt gacaaaaaag ccaggaagtg gtaaaaaacg
     1861 aaccgacaaa aagccaaacg ctcagaagat tatttttttg catttttcaa aaataaatcg
     1921 agttcttcta aattatgaaa tgtatatacc tgttgataaa gagaaaatcg tatttcccag
     1981 ttcgcatcga ttcgtagcca atgttgacta ttttcggctt tcgcaaccgg gagaaatcct
     2041 cttcgtttaa tgaacgccgc gatgagagga tgaagttctt catttacatc gataaaaaca
     2101 ggaagccctt gagtttgttc aaccccatag tcgaaggaaa aagggttgaa tttttttccg
     2161 ttgtgctcga aataatcctc gagcagggaa ctggcgaaaa tttcgttggg cactcccgaa
     2221 actatgggtt tatcggtggc cataagtatg atttcatcgg cctgccgaaa agccaaaggc
     2281 acgtcatgcg tagaaattag aatggttttg ttttgctgat gagagatgtt tgcaagcagt
     2341 tgcataattt cgattcggct cgggaaatcg agaaaagcgg ctggttcatc taaaaagata
     2401 tagggcgttt gttgagccaa agctttggct atcattactt tttgcttttc tccatcactg
     2461 aggtggtcaa aaaattcggc agccttgcag tggataccca ccatctggag tacatcgtgc
     2521 acaac
//
LOCUS       DAORPD010000164         2522 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_7414_length_2522_cov_2.214430, whole genome shotgun sequence.
ACCESSION   DAORPD010000164 DAORPD010000000
VERSION     DAORPD010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7414_length_2522_cov_2.214430"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(129..1286)
                     /locus_tag="PK976_02705"
     CDS             complement(129..1286)
                     /locus_tag="PK976_02705"
                     /inference="COORDINATES: protein motif:HMM:TIGR04350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HRC66830.1"
                     /translation="MANFDSIIPREGTSCVKYDLRNQVFGTEEVVPMWVADMDFATPE
                     FILQALHRRLNHPLLGYSFRAQEFNESLANWLFRRHALSIDPQIVSFSPGVVTGLMVL
                     TLAFTNPGDKIIVQPPVYPPFFEVPRAHERIVINNPLKFDGERYSMDFDDLREKAPGV
                     RMLILSNPHNPVGRAWERRELEQLSEICIQNDIMIVSDEIHSDIIFPGYRHTPLLSLD
                     KEIGMRSASCFAPSKTFNIAGLSTSAILIPDPQKRKIYNDTLERFHLQMGNLLGNIAF
                     EAAYNQGEDWLEELLDYLHQSVRIATHYFEEHLSPIHLVQPEATYLLWLDFRGLHIPD
                     DELHAHLIHKAHLGLNRGEEFGIEGKGFMRMNIACPHAILEKALESLKNIL"
     gene            complement(1350..2459)
                     /gene="gcvT"
                     /locus_tag="PK976_02710"
     CDS             complement(1350..2459)
                     /gene="gcvT"
                     /locus_tag="PK976_02710"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004330094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HRC66831.1"
                     /translation="MKNTPFTKFHIALGAKMVPFAGYNMPIQYEGVISEHLTVRKSVG
                     VFDVSHMGEIWVKGPKAFDLLQYITSNDVSKLYDGKIQYSCMPNGRGGIVDDILVYRI
                     TAESYLLVVNAANMEKDWNWITQHASDRGMKIGTDVYNASEETGQLAIQGPLAMKSMQ
                     KLTSESILDMEYYSWKNLEFAGVKNILFSTTGYTGSGGCEIYMPNEAADTIWNAVFEA
                     GKEFGIKPIGLGARDTLRLEMGFCLYGNDIDDTTSPLEAGLGWITKFTDTKNFIDKEY
                     LLQQKKEGVKRKLVGFVLKEKGIPRQHYSICNAAGNKIGEVTSGTMSPSLNIPIGMGY
                     VPAELSQPGSEIFIEIRGKLIKAEVVKLPFYKGEQ"
BASE COUNT          718 a          523 c          518 g          763 t
ORIGIN      
        1 tttgtgaagt aatttttacc ccaagacgtg caaatacgtt tatttcggct tcttcccata
       61 agggttccga atcgatcaac aggccatcga ggtcgaaaat agctgcctga aatgagttca
      121 ttctgttatt ataagatgtt ttttaaactt tcgagagcct tttcaagaat tgcatgtgga
      181 caggcaatat tcatgcgcat aaatcctttc ccttcgattc cgaattcttc tccccggttt
      241 aatcctagat gagccttatg aatcaggtgt gcatgaagtt catcgtcagg aatatgaaga
      301 ccccgaaaat cgagccacag caaataggta gcttcgggtt gtacgaggtg aatgggtgaa
      361 agatgttctt cgaagtaatg tgttgctatc ctcaccgatt gatgaagata atcgagtaat
      421 tcttcgagcc agtcttcgcc ttggttgtat gcagcttcga atgcaatatt ccctaaaagg
      481 tttcccattt gcaaatggaa gcgttcgagg gtgtcgttgt aaattttgcg tttttgagga
      541 tcagggatca aaatggcact ggtggagagt ccggctatat tgaaagtctt gctgggtgca
      601 aagcagctag cagaacgcat gccaatctcc ttgtccagcg agagtaaggg agtatggcga
      661 tagcccggaa atataatatc ggaatgaatc tcgtccgaaa caatcatgat atcgttttgt
      721 atacaaatct cggaaagctg ttctaattct cgtctctccc atgcacgtcc aacaggatta
      781 tgtggattcg acaggataag cattctgacg cctggggctt tttcgcgcaa gtcatcgaaa
      841 tccatcgaat atctttcccc atcgaatttc aatggattgt ttatgacaat tcgttcgtga
      901 gctctgggaa cttcgaaaaa tggcggatag acaggaggtt gaactatgat tttatcacct
      961 ggattggtaa aagccagagt tagaaccata aggcctgtga ctactcctgg actaaagctc
     1021 acaatttggg gatcgataga tagggcatga cggcggaata accaattagc aaggctttca
     1081 ttgaattctt gcgctcgaaa agaataaccc aataacggat gattcaaacg ccgatgcaag
     1141 gcttgcaaaa taaattcggg ggtagcaaaa tccatatcgg ctacccacat tggtactacc
     1201 tcctcggtac caaaaacttg attgcgtaaa tcatatttca cgcatgaggt accttctcgt
     1261 ggaataatcg aatcgaaatt agccattgtg acataatgaa tgaaaacatg agagcaaaaa
     1321 aacgctattt tgctctttaa aattctggtt tattgttctc ccttataaaa gggtagtttt
     1381 actacttctg ccttaataag ttttcctctg atttcgataa aaatctcact tcccggttgg
     1441 gataattcgg caggcacata gcccattccg attgggatat tgagcgaagg cgacatggtt
     1501 ccagaagtaa cttctccgat tttgtttccg gcagcgttac aaatggagta atgttgacgg
     1561 ggaattcctt tttctttcaa tacaaaacca actaatttgc gtttcacgcc ttccttcttt
     1621 tgctgaagga gatattcttt atcgatgaag ttctttgtat cggtaaattt tgtaatccat
     1681 cccagaccag cttccagagg ggaagtggta tcgtcgatat cattgccata taggcaaaat
     1741 cccatttcga gtctcaaagt gtcgcgagct cccaagccga taggttttat cccaaattcc
     1801 ttacctgctt cgaaaacggc attccaaatc gtatcggctg cttcgttggg catataaatt
     1861 tcacatccgc cacttccagt atatcctgtt gtagaaaaca aaatattttt cactccggca
     1921 aattcaagat ttttccatga ataatattcc atgtcgagaa tggattcgga ggtgagtttt
     1981 tgcatggatt tcatggccag tgggccttgt atggcaagct gcccggtttc ttcggaagca
     2041 ttgtaaacat cagttccaat tttcataccc ctatctgaag catgctgggt aatccaattc
     2101 caatccttct ccatattggc tgcattaacc acaagaagat aagattctgc agtaattctg
     2161 taaaccagaa tatcatccac tatcccacct cttccgttgg gcatgcacga atattgtatc
     2221 ttgccatcgt aaagttttga aacatcattg gaagtaatat actgaagcag atcgaaagct
     2281 ttagggcctt tcacccatat ctcacccatg tgagatacat cgaaaacacc tacgcttttc
     2341 cttacggtaa gatgttcgga tataactcct tcatattgaa tgggcatatt ataaccagca
     2401 aaaggtacca tctttgctcc taaggcaatg tgaaattttg taaaaggagt atttttcatg
     2461 tttttaaata ttttaatagc tgaattcaag tcacaaatgc aactttttgg ttagacaaat
     2521 tt
//
LOCUS       DAORPD010000165         2511 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_7456_length_2511_cov_2.028502, whole genome shotgun sequence.
ACCESSION   DAORPD010000165 DAORPD010000000
VERSION     DAORPD010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7456_length_2511_cov_2.028502"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="PK976_02715"
     CDS             complement(<1..521)
                     /locus_tag="PK976_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRC66832.1"
                     /translation="MVSNDKYDIQNILSQYFGYKSFRPLQEDIIRSVLGRKDTLALLP
                     TGGGKSICYQIPALAMEGVCLVVSPLIALMKDQVETLKKKGVEAEAVFSGMSSYQIEK
                     IFDDAAYGNLKFLYLSPERLITSSFRDRLPYMKLNLIAVDEAHCISQWGYDFRPPYLR
                     IAEIREFHQETPIL"
     gene            694..2238
                     /gene="lepB"
                     /locus_tag="PK976_02720"
     CDS             694..2238
                     /gene="lepB"
                     /locus_tag="PK976_02720"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HRC66833.1"
                     /translation="MDPLVYIILAFVFFPVFLLSIFKDVGIAPWKTLIPFYNYYLWNK
                     MIGKRLYWFLLLFVPFINVFMVFLMEVEIAKCYQKYDLGHQALAVLFPVIYLPYLGLS
                     AKEKYLEPSKRPVIKKTALREWVDALIFAVVAALIIRTFIFEAYTIPTPSMEKTLLVG
                     DYLFVSKMSYGPKVPNTPLSFPFVHHTLPFTQYSKSYVEWIKLPYYRFPGFGKVKHYD
                     PVVFNYPSGDTVVLERQNEDYYRIVRMAEEEFKMRMGSRYREGMGREAVWHTYHVVAR
                     PVDKRENYIKRCIALPGDTIQIINRQVYLNGKELPDPPLLQFNYLINTEGRGLSRRVL
                     ERMDISKEDMGWFQQYAILPLTKDKVQQISRIPGVIEVKPQLAPAGEWSPDIFPFDSA
                     YPWNVDNFGPLYIPKQGNEVTLTLHNLPLYKRIIEVYEKNRLVVKGNKIFINGEEANS
                     YRFQQNYYWMMGDNRHNSADSRYWGFVPEDHIVGKAVFVWLSLDKDKSLTEGKIRWKK
                     LFRIPR"
BASE COUNT          725 a          480 c          509 g          797 t
ORIGIN      
        1 agtatggggg tttcttgatg gaattctctg atttcagcaa tacgaagata tggaggcctg
       61 aagtcgtaac cccattgcga aatgcagtga gcttcgtcga cggctatcag atttaatttc
      121 atatagggca atcggtctcg aaatgaggaa gtgataagcc tttcgggtga taaatataag
      181 aatttcaaat tgccataagc tgcgtcgtcg aaaatttttt cgatctgata tgaactcatg
      241 cccgaaaaaa cagcttcggc ttcgactcct ttttttttga gagtttcaac ctgatctttc
      301 atcaaggcta tcagaggcga aaccactaag caaacccctt ccatggctaa ggcaggaatt
      361 tgataacaaa tactcttccc tcctcctgtc ggtaataacg caagtgtatc tttcctcccc
      421 aataccgacc gaataatgtc ttcctgcaat ggtctgaacg atttgtaccc aaaatattgg
      481 gaaaggatat tttgtatgtc gtatttatcg ttactaacca tttttttact ctatatcaaa
      541 agtatattga cagataaaat tcatacacct tctgggtttg ttcctctact aaggagaaca
      601 taaagtcagg aatcggattc cttcaaaatt actatcattg caacaaattt acacatgtat
      661 cgttaaaact gaatatcaat acataaagaa tatatggacc ctttggtata catcatttta
      721 gcctttgtct ttttcccggt ttttttattg tcaatattta aggatgttgg tatagcacca
      781 tggaaaacac ttatcccttt ttataattat tatttatgga ataagatgat agggaaacga
      841 ctttattggt ttttattgtt gtttgttcct ttcatcaatg ttttcatggt tttcctgatg
      901 gaggtggaga ttgccaagtg ttatcagaaa tatgatctgg gtcatcaagc attggcagtc
      961 ttgtttcctg taatatatct accttatttg ggattatcgg ccaaggaaaa atatctcgag
     1021 ccttcgaaac ggcctgtgat aaaaaaaact gctttgagag aatgggtcga cgctttaatt
     1081 tttgcagtag tagctgctct cattattcgt acgtttattt tcgaggcgta caccatccct
     1141 actccatcga tggaaaaaac gcttcttgtt ggcgattacc tttttgtaag caaaatgtct
     1201 tatgggccta aagtacccaa tacaccctta tcgttcccct tcgttcatca cacacttcct
     1261 ttcactcaat attcaaaatc gtatgtcgag tggattaagt taccctatta tcgatttcca
     1321 gggtttggta aagtaaagca ctatgaccca gttgtattta attatccatc gggagatact
     1381 gttgttttgg agcgtcaaaa cgaagattat tatcgtattg tgaggatggc cgaggaagaa
     1441 ttcaaaatgc gcatgggaag tcgttacagg gaagggatgg gcagagaagc ggtatggcat
     1501 acttatcatg tagttgctcg tccggtcgat aaacgtgaaa attatatcaa acgctgtatt
     1561 gctttacctg gtgatacgat acaaattatc aacaggcaag tttatctcaa tgggaaagaa
     1621 ttacctgacc cacccttgtt gcaattcaat tatttgataa atactgaggg acgtggcttg
     1681 agtcggcgag ttctcgagcg catggatatt tcgaaagaag acatgggatg gtttcaacaa
     1741 tatgccatac tgccccttac caaagataaa gttcaacaaa ttagcagaat acctggtgta
     1801 atcgaagtaa aaccgcaatt ggctcctgct ggcgaatggt cacccgacat cttccccttc
     1861 gattcggctt atccatggaa tgtcgataat ttcgggcctt tgtacattcc gaaacaaggc
     1921 aatgaggtca cccttaccct ccataatcta cctttataca agcgaatcat cgaagtgtac
     1981 gaaaaaaacc ggctagttgt taaaggaaat aaaattttta tcaatggaga agaggctaat
     2041 tcgtatcgtt ttcagcagaa ttactattgg atgatgggtg ataaccgaca taattcggct
     2101 gattcccgtt attggggctt tgttcccgag gatcacatag tagggaaagc cgtatttgtt
     2161 tggctttctc tcgataaaga taaatcgctc accgaaggaa aaattaggtg gaaaaagtta
     2221 tttcgcatcc ccagataagg agtgtttcga tcaaaaggtt tgagttggaa tactctctta
     2281 ggtaattacg atatcgttaa gaactctcga catttgggta aagctctggt catcatctaa
     2341 cgacatttcg agcttgtgtg tatgatagcg gatttgttag aaataaggtt atagaagatt
     2401 tttagcaggc attgaatact tcttcgacca actccgaatt attggtcttc ttccgtttct
     2461 ctgctatgac atctaccagc gttttactcc gggctgccta gtttgagata t
//
LOCUS       DAORPD010000166         2506 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_7483_length_2506_cov_2.346797, whole genome shotgun sequence.
ACCESSION   DAORPD010000166 DAORPD010000000
VERSION     DAORPD010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7483_length_2506_cov_2.346797"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /gene="rnc"
                     /locus_tag="PK976_02725"
     CDS             <1..709
                     /gene="rnc"
                     /locus_tag="PK976_02725"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HRC66834.1"
                     /translation="SNDKKFYIALKNIFGFWPENIFLYKLALRHKSAALEFVNGERLS
                     NERLEFLGDAILDAVVAHYLFQLFPLRDEGFLTQMRSKIVNRQQLNALSHHLGLDNYI
                     VASREINHVRSINGNALEALIGALFLDKGFKFAEKVIINRILKIHFDLDELQNKDFNF
                     KSKLIEWGQKEKHQVNFLLRGETGKGMNKQYLVEVLIDQKKISEGRDFSIKGAEQIAA
                     EKAILRMKEIFSTSSFN"
     gene            770..1255
                     /locus_tag="PK976_02730"
     CDS             770..1255
                     /locus_tag="PK976_02730"
                     /inference="COORDINATES: protein motif:HMM:NF033205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPExxxVDY family protein"
                     /protein_id="HRC66835.1"
                     /translation="MPKTLIESELNENYRIYSLSTDLPDYKLCYSLNRQLKWDFRRRS
                     HLHLTFVKKNSPRQFSLFQYELDQFARFFLLYPFSEQIIVLPNCFFILQGCIHLSTEQ
                     KILETIRWIDGIHSFYFIDIQNPNASSAIHDFFDSLIYDLEYHLLQLDVSRAANAMIR
                     Y"
     gene            complement(1677..>2506)
                     /locus_tag="PK976_02735"
     CDS             complement(1677..>2506)
                     /locus_tag="PK976_02735"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRC66836.1"
                     /translation="KIPWMADLRDPWTDIYYYDEFRHLPWIKHKDAQLELSVLENADA
                     ILVVSPSIKNLFLSKSAKISEDKIHVIPNGFDDEDFNSISNQPKNESFTITYTGTLST
                     AYNIDALIHAVKHLKNSNPNIKIHIQFIGKISPEILQSLKNNGLENMLSIIDYLPHRE
                     AITQMMAADVLLLAIPKINENKGILTGKLFEYMATGNPILGIGPPDGDASKILDETHS
                     GIMFDYDQNNEVLSFLRSHYEIWQKGDNLRRSSPKVEKYSRKALTAQLAQIIDQFAR"
BASE COUNT          734 a          449 c          517 g          806 t
ORIGIN      
        1 ctccaacgat aaaaaatttt acatcgcttt gaaaaatatt ttcgggttct ggcccgaaaa
       61 tattttttta tataaactgg ccttgaggca caaatcggcc gcccttgagt ttgttaatgg
      121 tgaacgcctc agtaacgaac gactcgaatt tttgggcgat gccatcctcg acgccgttgt
      181 ggctcactat cttttccaac tgttcccttt acgtgatgag ggcttcctta ctcaaatgcg
      241 tagcaaaata gttaatcgcc agcagctcaa tgcactttct catcatcttg gcctcgacaa
      301 ttatatcgtg gccagccgcg aaattaatca tgtcagaagc atcaatggaa atgccttaga
      361 agcacttata ggggcacttt tcctcgataa aggattcaaa tttgccgaaa aggtcattat
      421 caaccgaatc ttaaaaatac atttcgatct cgatgaactt caaaacaagg attttaattt
      481 caagagcaaa ctcatcgagt gggggcaaaa agagaaacat caggtgaatt ttcttttaag
      541 aggagaaacg ggcaaaggta tgaacaagca ataccttgta gaagtattga ttgatcagaa
      601 aaaaattagc gaaggtcgcg acttttccat taagggagcc gaacaaattg ccgccgaaaa
      661 agctatcctt cggatgaagg aaatcttttc aacctcctca tttaactgat taaattcttt
      721 tattaggcaa aactattaat tttgggcttg ctcctctttt tgcttcatca tgcctaaaac
      781 cttaatcgaa tcagaactta acgaaaatta taggatatat tctctgagca ctgatctccc
      841 ggattataag ctgtgttata gcttaaaccg tcaattgaaa tgggatttca gaagacgcag
      901 ccatctgcat ctgacctttg ttaaaaaaaa ttcaccacgt cagttttctc tttttcaata
      961 tgaactcgat cagtttgctc ggtttttctt gctttaccca ttctcggaac aaataattgt
     1021 gctgcccaat tgtttcttta ttcttcaagg atgcattcac ctcagcacgg agcaaaaaat
     1081 attggaaacc atccgctgga tagatggaat tcattccttt tactttattg atattcaaaa
     1141 ccccaatgcc tcttcggcca tccatgattt tttcgactca ttgatttatg atctcgaata
     1201 tcatttactt cagctcgatg tgtctagggc ggccaatgca atgattcgct attagaagta
     1261 ccctttttga tgaataatga cgaagggaat gatggttgat tcaacaatgt tgctaagaat
     1321 atatgattga ttattaatgt aataaggtga ggattagaaa ttaacatggg atttcttggt
     1381 ttgggttaaa gtattgtaag ctgaattcat gttgacatat tttaattcgt cgaaaaccta
     1441 atggtggctc agatatagga atgttgcgag tcgtgtaata tacaagaatg aatctatcaa
     1501 ggtattggaa tagaatagcg gttacaggaa tctgaccgct tgaattggaa gaacagaatg
     1561 caggttgctt ttggaattgg ctaaattgaa tcgaaaggat ttctctggat tcttataggt
     1621 ggctaattat ctatatcaat tcgaatcagg atgatgatat agaacaagga aaatttttat
     1681 cgagcgaatt gatctatgat ttgggctaat tgagcggtca atgctttgcg tgaatatttt
     1741 tcgactttag gggagctcct tcgaaggtta tccccttttt gccagatctc ataatgactg
     1801 cgtaaaaatg ataacacttc gttgttctgg tcataatcga acatgattcc cgaatgggtt
     1861 tcatcgagta tttttgaggc atctccgtca ggaggcccta tgcccagaat cgggtttcca
     1921 gtagccatgt actcgaaaag ttttcctgtc agaattcctt tattttcgtt gatttttggt
     1981 attgccaaga gcaagacgtc agcagccatc atttgagtaa tggcttcacg gtgaggaaga
     2041 taatcgatta tggacaacat attttccaaa ccattgtttt tcaaagattg cagaatttcg
     2101 ggagaaattt ttccaatgaa ttggatatga attttaatat ttgggttgct gtttttgaga
     2161 tgctttacgg catgaataag agcatctata ttataagctg ttgacagagt cccggtatag
     2221 gtaattgtaa aagattcgtt ctttggctga ttggaaatac tgttgaaatc ttcatcatcg
     2281 aagccgttgg gaatgacgtg aattttatcc tctgagattt tggctgattt ggaaaggaaa
     2341 agatttttta tggatgggct caccacgagg atggcatcgg cgttttcgag aaccgaaagt
     2401 tcgagttggg catctttgtg ttttatccac ggaagatgtc ggaattcgtc gtaatagtat
     2461 atatctgtcc atggatcacg aagatcggcc atccatggaa tttttg
//
LOCUS       DAORPD010000167         2487 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_7563_length_2487_cov_2.068668, whole genome shotgun sequence.
ACCESSION   DAORPD010000167 DAORPD010000000
VERSION     DAORPD010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7563_length_2487_cov_2.068668"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1510
                     /locus_tag="PK976_02740"
     CDS             <1..1510
                     /locus_tag="PK976_02740"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC66837.1"
                     /translation="SQFSLEAHAPVLQEGEISVNDSLGNNNQRFDAGESVYVNIPVIN
                     IGSSAAYNLIGTLTTADPYLLISTKEATYGDILPLGSQSQAFLISALPTVPPGYQAMI
                     TLTLMGDFGWTASYDFTLTIGLIPVLVIDLDGNNNSGPTMQAALNQLNVANEYTTSFS
                     TDLSKYKSLFVCLGVYPNKHILSAAEGLVLKNYLNQGGRIYMEGGDTWYYDQQSTPTP
                     LHPMFQIKGLKDNGGTLTTIAGKTGTFTEGMSFVYNGDNNYIDKIEPINQAFTVFMNT
                     SPSYIVTIANDPGSVYKTIGSSNEFGGLTDNVSPSTKKDLMLQYLTFFDIFGSPYWGN
                     FVGIPTTIGINDTVEFFDLSSTTFTSRIWSFPGGEPSTSTELNPKIVYPSMGTYDVTL
                     EVSSSDSTIIIIRNDYISVYDYTGINPQSVSTEIILYPNPLSRNFGALNIRSSNANIQ
                     SIRIFNSTGMEVFSQMNYMESSPISLNHLKPGLYLVEIKTDQGIATQKLMIR"
     gene            complement(1525..>2487)
                     /gene="queG"
                     /locus_tag="PK976_02745"
     CDS             complement(1525..>2487)
                     /gene="queG"
                     /locus_tag="PK976_02745"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HRC66838.1"
                     /translation="KLAFMDTLSIKSKIRQQAFDIGFSKCGFAVSRFLTSDFEHYKKQ
                     IALGFHADMLYLEKEAEKRFHPLLVHPDIRTVIVLLAGYKTNARQKDGAFYSISDYAL
                     IPDYHKIMKEGLQKLSCFIENEFHSDVLINFVDTGHISEKAWAREAGLGNFGKNSILL
                     TPEGSYFFIGVILTDLVIIPDEAFDEDLCKNCDLCIKSCPTQAIHQPYAVDARLCISY
                     HTIENKGHIPSNIIEKIGTQIYGCDICQKVCPWNRHQTVNSVLFSPLPPLLDMKAEDF
                     ESVTETDFRERFHESPMVRLGHRRYMRNIEAVKNMAATERKRPY"
BASE COUNT          758 a          527 c          444 g          758 t
ORIGIN      
        1 aagccaattt tcgctcgagg cacatgcccc agttttacag gagggagaaa taagtgttaa
       61 cgacagcctt ggaaacaaca atcagcgctt cgatgcagga gaatctgttt atgtgaacat
      121 tcctgttatc aatattggat cttcagcagc atataatctc attggaactc tcaccactgc
      181 agatccttat cttctaatta gtacgaagga agctacttat ggagatatat tgccccttgg
      241 ttcccaatct caagctttcc taatatcggc tcttccaacc gtccctccag gttatcaggc
      301 catgattact ttgacgctca tgggagattt tggttggact gcttcgtatg actttacctt
      361 gacaattggc cttattcctg ttttagtgat cgatcttgat ggcaacaaca actccgggcc
      421 aactatgcaa gccgcattga atcaactcaa tgtcgcaaat gaatatacta cctccttctc
      481 caccgattta agtaaataca aatccttatt cgtatgtcta ggagtttatc ctaacaagca
      541 cattctttct gctgccgaag gccttgtgct taaaaactat ttgaatcaag gtggtagaat
      601 atatatggaa ggtggagata cctggtatta tgatcaacaa agcactccta ctcctttaca
      661 tccaatgttt caaatcaaag ggctgaaaga caatggaggt acgcttacga caattgccgg
      721 aaaaacaggc acatttaccg aaggaatgag ctttgtttac aatggggaca acaactatat
      781 cgataagata gaaccaataa atcaagcatt taccgtattt atgaatactt caccctctta
      841 tatagttact atagccaatg atcctggttc tgtttacaag accattggtt cttctaatga
      901 atttggggga cttaccgata atgtttctcc ctcaactaaa aaggacttga tgctacaata
      961 tcttaccttt ttcgatatct tcggaagccc ttactgggga aacttcgtcg gaattccaac
     1021 tactatcgga atcaacgata cggttgaatt cttcgatctc tccagcacta catttacgtc
     1081 gcgtatatgg tctttcccag gaggagagcc ttcaacatcg accgaactca accctaaaat
     1141 agtttatcct tccatgggaa cttatgatgt tacgctcgaa gtatcatcat ctgattctac
     1201 tataattatt atccgaaatg actatatttc agtatatgat tatactggca tcaatcccca
     1261 atcagtttcc accgagatta ttctttatcc caaccctcta agtcgaaatt tcggtgcttt
     1321 gaatattcgt tcctcgaatg ccaatattca atctattcga attttcaatt cgacgggaat
     1381 ggaggtcttt tctcaaatga attatatgga aagcagtccc atttcactga atcatttgaa
     1441 accaggttta tatctggtag aaataaaaac agatcaggga atagcgacac aaaaactcat
     1501 gattcgataa ataacgagca gtttttaata tggccgcttc ctttcggtag cggccatatt
     1561 ttttacagcc tctatatttc gcatgtatct tctgtgtcct aacctgacca tggggctttc
     1621 atgaaatctt tccctaaaat cagtctccgt aacagattcg aaatcttcgg ctttcatatc
     1681 caataggggt ggcaaaggcg aaaacaaaac agaattcaca gtttgatgac gattccatgg
     1741 gcaaaccttc tggcatatat cgcaaccata aatctgggtg cctatctttt ctataatatt
     1801 ggagggaata tggcctttat tctcgatggt atgatacgaa atgcacaatc gggcatccac
     1861 agcataaggt tgatgtattg cttgtgtagg acaactcttt atacataaat cgcaattttt
     1921 gcataaatct tcatcaaagg cctcatcggg gataataacc agatcggtca aaattacacc
     1981 aataaaaaaa taagaacctt cgggggttaa caaaatactg ttctttccga agtttcctaa
     2041 acccgcctca cgagcccatg ctttttccga aatatgacct gtatctacaa aatttattaa
     2101 aacatctgaa tgaaattcat tttcgatgaa acaagataat ttttgcaagc cttctttcat
     2161 tattttatga taatcgggta tcaaagcata atctgaaatg gaataaaaag ctccatcttt
     2221 ttgccgtgca ttagttttat agccggctaa aagaacaata acggtccgaa tgtcgggatg
     2281 aacgagtaaa ggatggaatc ttttctcggc ctctttttcc agatacagca tatcggcatg
     2341 gaatcctagg gcaatttgct tcttataatg ttcaaaatcg gaggtgagga atctactaac
     2401 tgcaaagcca catttggaaa agccaatatc aaaagcttgt tgacgaatct tcgattttat
     2461 ggataaagta tccatgaaag ccaattt
//
LOCUS       DAORPD010000168         2485 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_7571_length_2485_cov_2.011934, whole genome shotgun sequence.
ACCESSION   DAORPD010000168 DAORPD010000000
VERSION     DAORPD010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7571_length_2485_cov_2.011934"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..810)
                     /locus_tag="PK976_02750"
     CDS             complement(<1..810)
                     /locus_tag="PK976_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HRC66839.1"
                     /translation="MRQLKITKQVTNRETASLDKYLQEIGKVELLTAEEEVALAQRIK
                     QGDQVALEKLTKANLRFVVSVSKQYQNQGLSLPDLINEGNLGLIKAAQRFDETRGFKF
                     ISYAVWWIRQSILQALAEQSRIVRLPLNKIGSLNKINKAYAKLEQTFEREPNYEEIAQ
                     QLEITENEVKDSLRNSGRHISMDAPLVEDEDNTMLDVLRADDTPSPEAGLIYESLCHE
                     IQRVISTLSPREADVIRLYFGLEGTHPLTLEEIGEKFDLTRERVRQIKEKAI"
     gene            complement(874..>2485)
                     /gene="pnp"
                     /locus_tag="PK976_02755"
     CDS             complement(874..>2485)
                     /gene="pnp"
                     /locus_tag="PK976_02755"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014775183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HRC66840.1"
                     /translation="LMVAATLDNIVMVEGEMKEISENELLQALEYAHEVIKVQCLAQI
                     ELSKKVGACQNKRQYNHEVNDEELKQRVHDFCYDKIYDLASHPTNKHERTEKFEQILN
                     DFIETIPVEERTGKELMVKRYYYRTQWEAVRNVILERRVRLDGRQLDEIRPIWSEVDY
                     LPTAHGSAIFTRGETQSLTTCTLGTKLDEQTIDGAVLEGKVNFILHYNFPPFSTGEVR
                     TTRGTSRREIGHGNLALRALKPVMPLPEENPYTIRVVSDILESNGSSSMATVCAGTLA
                     LMDAGVKIRKPVSGIAMGLITDEQTGNYAILSDILGDEDHLGDMDFKVAGTEVGITAC
                     QMDIKVSGLSAEILNQALVQARKGRLHILREMRKTISSPRPDYKPHVPRIVQMTIPRE
                     FIGAVIGPGGKIIQDIQHETNSTIVIEEVGEFGIIDIVAVDRDSIEAVKKKIKSIIAI
                     PEIGEVYTGKVKTIVPFGAFVEILPGKDGLLHISEIDWKRINDIESVLKVGDEVTVKL
                     IDIDRTTGKLKLSRKALLPKPGNNNTKQ"
BASE COUNT          634 a          552 c          479 g          820 t
ORIGIN      
        1 gattgccttt tccttgatct ggcggacgcg ttcgcgagtc aggtcaaatt tttcgccaat
       61 ttcttcgagg gtaagtgggt gcgttccttc cagtccaaaa taaaggcgaa tcacatctgc
      121 ctctctgggt gaaagggttg aaattactct ttgaatttca tgacatagag attcataaat
      181 tagacctgct tcagggctgg gggtatcatc ggccctaaga acatccagca tagtattgtc
      241 ttcgtcttct accagagggg catccatcga aatatggcgg ccagaatttc gcaatgaatc
      301 ctttacttcg ttttcggtta tctccaattg ctgagcgatt tcctcataat tgggttctct
      361 ttcgaaagtt tgttctaact tggcataagc cttatttatt ttattcaacg aacctatctt
      421 gttgagaggt aggcgcacaa tacgtgattg ttcggcaagt gcctgaagaa tactttgcct
      481 gatccaccat accgcataag agataaattt aaaaccccgg gtttcatcaa aacgctgggc
      541 tgccttgatc aaacccaaat taccttcatt aatcagatca ggaaggctta aaccttggtt
      601 ttgatactgt ttggaaacag atactacaaa acggagattg gctttggtta atttttcgag
      661 tgctacctga tcgccttgtt taatccgctg cgccaaagca acttcttctt cggctgtaag
      721 tagttcaact ttcccaattt cttgcaaata cttgtcgagt gatgcagttt ctcggttagt
      781 aacctgtttg gtaattttta gctgcctcat attaggattt ttttaccatg tttcatttta
      841 cgacttatcc ctataaaggt ttcaaaaatt caattattgt ttggtattgt tattccctgg
      901 ttttggcagg agagcctttc gcgagagttt taattttcct gttgttcggt cgatatcgat
      961 gagtttcacg gtaacttcgt ctccaacttt cagaacactc tctatgtcat taattcgttt
     1021 ccaatcaatt tctgatatat gtagcaagcc atccttaccc ggaagtattt cgacaaaagc
     1081 gccaaaaggc acaatggttt tcactttgcc cgtatatact tcaccgattt ctggaatagc
     1141 aatgatggat ttgattttct ttttaacagc ttcgatcgag tcgcgatcga cggctacaat
     1201 atcaataatt ccaaattctc ctacttcttc tattactatt gtagaattag tttcatgttg
     1261 aatgtcctga attatctttc ctcctggacc aattactgca ccaatgaatt ctcttggaat
     1321 agtcatttga acgatacgtg gtacatgggg tttataatcg ggccgtggag acgaaatagt
     1381 tttgcgcatc tcccgaagaa tatgcaaacg cccttttcga gcctgtacga gtgcctgatt
     1441 cagtatctct gccgataaac ccgatacttt aatatccatt tggcaggctg taatgccaac
     1501 ttcagtccca gcaaccttaa aatccatgtc tcctagatga tcttcatctc cgagaatatc
     1561 ggaaagaatg gcataattac ccgtttgttc gtcggtaatt aatcccatag cgatacccga
     1621 cactggtttt cttattttta caccagcatc catcaaagcg agggttcctg cgcaaacagt
     1681 agccatagag gatgaaccgt tcgattccaa gatatctgat accactctta tggtataggg
     1741 gttctcctca ggtaatggca tcacaggttt aagtgctctc agtgcaaggt tgccatgccc
     1801 aatttctcta cggctggttc ctcgggtcgt tcttacttct cctgtcgaaa agggtggaaa
     1861 attataatga agaataaaat tgaccttccc ttccagaact gcgccgtcaa tagtttgctc
     1921 gtcgagcttg gtcccaagtg tacaagtggt tagcgactgc gtttcgcctc tcgtaaatat
     1981 ggctgaacca tgtgccgtag gcagataatc tacttcagac catataggcc ttatttcatc
     2041 caattgcctt ccatcgaggc gtactctcct ttctaatata acattacgaa ctgcttccca
     2101 ttgggtacga taataatacc gtttcaccat caattcctta ccagttcttt cttccacggg
     2161 gatggtttcg ataaaatcat tcagtatttg ttcgaatttt tccgtacgtt cgtgtttatt
     2221 agtaggatga gaagccaggt cataaatttt atcatagcaa aaatcgtgta ctctttgctt
     2281 aagttcctca tcatttactt cgtggttata ttggcgcttg ttttgacaag caccaacttt
     2341 tttcgatagt tcgatttgag caaggcactg cactttaatt acttcatgag catattcaag
     2401 agcctgaagt agttcatttt ccgaaatttc cttcatctcg ccttcaacca tcacgatatt
     2461 atcgagagta gcagctacca taagg
//
LOCUS       DAORPD010000169         2474 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_7616_length_2474_cov_2.236048, whole genome shotgun sequence.
ACCESSION   DAORPD010000169 DAORPD010000000
VERSION     DAORPD010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7616_length_2474_cov_2.236048"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="PK976_02760"
     CDS             <1..648
                     /locus_tag="PK976_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRC66841.1"
                     /translation="GSEYHSTDGSIKKAGTIGNIGCTSFFPSKNLGCYGDGGAIFTND
                     DELANMLRGIANHGMFARYYHDFVGVNSRLDSIQAAILKVKLPHLDEYISARQKAAQY
                     YERAFADIPYLKLPIRHPKSSHVFHQYTLICNGISRDDLVNYLAKKDIPAMVYYPVPL
                     HLQKAYKSERYGKGDFPVTEMLSANVFSLPMHTELDEEQLQYICDQVLEFISRVK"
     gene            669..1247
                     /locus_tag="PK976_02765"
     CDS             669..1247
                     /locus_tag="PK976_02765"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRC66842.1"
                     /translation="MAKDYFAHETAVIDKGCVIGQGTRIWHFSHIMSGCTIGENCNLG
                     QNVVVSPKVVLGNNVKVQNNVSIYTGVICEDDVFLGPSMVFTNVINPRSAVNRRGHYA
                     QTIVKRGATIGANATIVCGHNIGEYAFVGAGAVVTREVLPYALVIGNPARQIGWMSEY
                     GHRLHFDEDNIAICPESHEKYKLENNQVFKIS"
     gene            1272..2219
                     /locus_tag="PK976_02770"
     CDS             1272..2219
                     /locus_tag="PK976_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRC66843.1"
                     /translation="MYNFALIGAGGYIAPRHMKAIKDTGNRLVAALDPNDSVGIIDSF
                     FPEADFFLQPERFDRHLDKLRRQGEKKIDFVSICSPNFLHDSHIRLALRNQANAICEK
                     PIVLNPWNIDALEDIENETGFRIYNILQLRLHPQIIALKEKIKNGPTDKIYDLDLTYI
                     TSRGKWYHYSWKGDVEKSGGLATNIGVHFFDMLGFIFGKIRLVAVHHSSPTVVAGFLQ
                     YDKARVRWFLSIDSKYLPKEIAAKGQRTYRSILIDGEEFEFSEGFADLHTRVYEDILS
                     GGGYGLEDARQSVEIVYQIRNAKPFGLTGDFHPYLLKVD"
BASE COUNT          719 a          409 c          566 g          780 t
ORIGIN      
        1 ggatccgaat atcactcaac agatggcagt ataaagaagg ctgggacaat aggaaatatt
       61 ggatgcacat cttttttccc aagtaaaaat cttggttgtt atggtgatgg aggggcaatc
      121 tttacaaatg atgacgagct tgcaaatatg ctgagaggta tagccaatca tggaatgttt
      181 gcacggtatt atcatgattt tgttggagtg aattcacgct tagacagcat tcaggcggct
      241 atcttgaagg ttaaattgcc ccatctcgat gaatatattt cagcccgcca aaaggccgct
      301 caatattatg aaagggcttt tgcagacatt ccctacctga agttaccgat tcgccatccc
      361 aaatcatcgc atgtttttca ccaatatacc ttgatttgca atggtatttc tcgggatgat
      421 ctagtgaatt atttggcaaa aaaggatatt cctgccatgg tttattatcc tgtccccctt
      481 catttacaaa aggcttataa aagcgaacgc tatggcaagg gcgatttccc tgtaacagag
      541 atgctctcgg ccaatgtttt ttccttaccc atgcataccg agctcgatga agaacaatta
      601 caatatattt gtgatcaggt tttagaattt atttcacggg tcaaataata ataaaaaatc
      661 tgtcgaaaat ggccaaagat tattttgcgc atgaaactgc tgtaatcgac aaaggatgtg
      721 tgattggcca gggcactcgc atctggcatt tttcgcatat catgtcgggt tgtacaattg
      781 gagaaaactg caaccttgga caaaatgtag tggtttcgcc caaggtagtt ctgggaaaca
      841 atgtcaaggt tcaaaacaat gtttccattt atactggggt aatttgtgaa gatgatgttt
      901 ttttgggtcc ttctatggtt tttaccaatg tcatcaatcc aaggagtgct gtcaatcgca
      961 ggggacatta tgctcaaacg atagtaaaac gaggagctac cattggggct aatgctacta
     1021 ttgtttgtgg acacaatatt ggagaatatg cctttgtggg agctggagcc gttgttactc
     1081 gcgaagtact accttatgcc ctcgtaattg gaaacccagc ccgacaaata ggatggatga
     1141 gtgaatatgg tcatcgtttg catttcgacg aagataatat tgctatttgc cctgaaagtc
     1201 acgaaaaata taaattagaa aataatcagg tattcaagat ttcttaaatt tttaatttta
     1261 tggaaaattt aatttacaat tttgccttga taggtgcagg gggttacatt gcacccaggc
     1321 acatgaaagc cattaaagac acgggcaatc gtttggtagc agctctcgat cccaacgaca
     1381 gcgtggggat tatcgattct tttttcccgg aagccgattt ctttttacaa cccgagcgtt
     1441 tcgaccgtca tttggataaa ttgcgccgac agggagaaaa gaaaatagat ttcgtaagca
     1501 tttgttctcc aaattttctg cacgattctc acatacgact ggctctgcgt aatcaagcca
     1561 atgctatctg tgaaaaaccc atcgtgctga atccttggaa catcgatgcc ttagaagaca
     1621 ttgaaaatga aaccggattc agaatctata atattcttca attgaggctt catcctcaaa
     1681 ttattgcatt aaaagagaaa atcaaaaatg gccctaccga taaaatctat gatcttgatc
     1741 ttacctatat tacctcaagg ggtaaatggt atcattattc atggaagggt gatgtggaaa
     1801 aatcgggtgg tttggccacc aatattggcg ttcatttttt cgatatgctg ggttttattt
     1861 ttggaaagat tagattggtg gccgttcatc attcgtcacc tacagttgtt gctggttttt
     1921 tacagtacga taaagccaga gtgagatggt tcttgagtat cgattcgaaa tatttgccta
     1981 aagaaattgc cgctaaaggt cagcgtactt atcgttcaat attaatagat ggtgaagagt
     2041 ttgagtttag tgaaggtttt gccgatttac atactcgagt ttatgaggat attttgtcag
     2101 gtgggggcta tggtttagaa gatgcacgcc agagtgtgga aattgtctat caaattcgta
     2161 atgcaaagcc ctttggactt acaggcgact ttcatccata tttgctgaag gtggattgat
     2221 gggtggttgg tgattagtga gttaaaattt gaaagttaaa agtttataaa gttaaaaagg
     2281 aggaagggag gaaaggagaa tggtggatgg tggatggtga ctggaatttg gagactgggc
     2341 gactgagaga cttgaattta taatttagtt gaaatttgaa acttgtaatt tggagttttt
     2401 tagttgtaag ttgttggttg tcagttatca gttggatttt gtcgatgttt gtttgtgatt
     2461 tgggatttgt aatt
//
LOCUS       DAORPD010000170         2468 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_7639_length_2468_cov_2.693742, whole genome shotgun sequence.
ACCESSION   DAORPD010000170 DAORPD010000000
VERSION     DAORPD010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7639_length_2468_cov_2.693742"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="PK976_02775"
     CDS             <1..248
                     /locus_tag="PK976_02775"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HRC66844.1"
                     /translation="IWRYLSGGNENTVWKFIRLAWSSVAFIAVAPMQDILELDYTARM
                     NTPGTSSNNWQWRFKKSDYNDRIVNKLIELTQLYDRD"
     gene            638..>2468
                     /locus_tag="PK976_02780"
     CDS             638..>2468
                     /locus_tag="PK976_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014298424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HRC66845.1"
                     /translation="MNLNNFTVKSQEIIQEAQFLAQEKQHGQIDTGHFLVALLEKDEQ
                     VTPFLLKKIGANPSFILQEGQKLLNTYPRVSGGELLLSRESSQLLQRAISIAREMGDD
                     YVAIEHLLLSLVAGNDAVASILKSQGVGRKDLEEAIQQMRKGSKVDSVTSDENYNALN
                     RFAINLNEMARKGKLDPVIGRDEEIRRVLQILSRRTKNNPILLGEPGVGKTAIVEGLA
                     FRIINGDVPENLKTKQIYSLDMGALIAGAKYKGEFEDRLKAVIKEVTSSEGEIILFID
                     EIHTLVGAGGGEGAMDAANIMKPALARGELRAIGATTLKEYQKYFEKDKALERRFQTV
                     MIDEPDAASAISILRGLRERYENHHKVRIKDEAIIAAVELSQRYITDRFLPDKAIDLI
                     DEAAAKLRLEINSLPEELETVERRIRQLEIEREAIKREGDEEKLEQLNQEIANLTEER
                     NVLRAKWQSEKNLIDQIQQKKREIEDYRLAAEQAEREGNYGRVAELRYGKMREAEQQI
                     EHLRKQLAEIQGDKPMINEEVSAEEVAEVVSKWTGIPVSRMLQSEREKLLHLEEHLHK
                     RVVGQDEAISAVADAIRRSRAGLQDPKRPVGSFIFLGTTGVGKT"
BASE COUNT          809 a          452 c          592 g          615 t
ORIGIN      
        1 gaatttggcg ctatttgtca ggaggaaacg aaaataccgt ttggaaattt attcgattgg
       61 catggagttc ggttgctttc atcgctgttg ctcccatgca ggatatcctc gaactcgact
      121 atacagcccg aatgaataca cctggaacat cgtccaacaa ctggcaatgg cgttttaaaa
      181 aatcggatta taacgacaga atagtaaata aactaatcga actgacacaa ctttacgata
      241 gagattgaaa aactattgtc gagcctcttt caggaatttt ccctcttata tcatgaattc
      301 atcatttttt gctgattctt cgatattttt gtgaagttat acgattagga tttttccata
      361 gactaatcta ctatcaaaat agaacgagaa agtgaatggc ttgaagattt tttgaaatct
      421 gataaaactg actgcttttg agttttttaa gttatagaaa agatttacaa taatgacatt
      481 ttgtcttatt tttagaagca attttatcga tttataagac attttgtcat aattatatcg
      541 catagggact cgataataag aatggcaagc caattgagta aagttgggaa aaacaaagat
      601 tagatgaaat tagataaaac aggaaggagc aaaagatatg aatctcaata attttacggt
      661 caaatcgcag gaaattattc aagaagccca gtttttagca caagaaaaac agcatgggca
      721 aattgacacg gggcattttt tggtagccct gctcgaaaaa gacgaacaag ttactccatt
      781 tttattgaaa aaaattggag caaatccatc gttcatactt caggaaggac aaaagctgtt
      841 aaatacttat ccaagggtaa gtggaggcga gttgttacta tcgcgtgaaa gcagtcagtt
      901 gttacagcga gccattagca ttgcccgtga aatgggagat gactatgtgg ccatagaaca
      961 tcttttatta tcgctggtag ctggcaatga tgccgtagcc tcgatattga aatcacaggg
     1021 tgttggtcgc aaagacctcg aagaagccat tcagcagatg cgcaaaggct cgaaggtgga
     1081 cagtgtgact tctgatgaga attacaatgc cttgaatcgt tttgccatca atttgaatga
     1141 aatggcacga aaagggaagc tcgatcccgt aattgggcga gatgaggaaa taaggcgtgt
     1201 gttgcagatt ctttcgcgac gcaccaaaaa caatcccatc ttgttaggag aacctggtgt
     1261 tggaaaaacg gctatcgtag aaggattggc ttttcgaatc atcaatggcg atgtgcccga
     1321 aaatctaaaa accaaacaaa tctattctct cgacatgggt gccttgatag cgggtgcgaa
     1381 atacaaggga gaattcgaag atcgtttgaa ggctgtcatt aaggaagtta ccagttcgga
     1441 aggtgagatc attttattca tagatgaaat tcatacattg gtaggagccg gtggaggcga
     1501 aggtgccatg gacgccgcaa atattatgaa gcctgctctt gcgcgaggcg aattgcgggc
     1561 aattggtgcc actaccttga aagaatatca gaaatatttc gaaaaggaca aggcattgga
     1621 acgtcgtttc caaacagtga tgatagatga acccgatgca gcttcggcta tttctatcct
     1681 cagaggctta agagaacgtt acgaaaatca tcataaagtc agaataaagg atgaagccat
     1741 catcgctgca gtcgagttgt ctcaacgtta tattaccgat cgttttctac cggataaagc
     1801 catcgacctg atcgatgaag ctgcagcaaa gttaaggctc gaaatcaatt cattgcccga
     1861 agaattggaa acggtggagc gtcggattcg tcagcttgag attgaaagag aagccattaa
     1921 gcgtgaaggc gatgaagaaa agctcgaaca gctcaatcag gaaatcgcca atcttaccga
     1981 ggaacgcaat gtattgagag ccaaatggca atcggagaaa aatctgatcg accaaatcca
     2041 gcaaaagaaa agagagatcg aagattatcg ccttgctgcc gagcaagccg aaagagaagg
     2101 aaactatgga agagtggccg aactccgata tggtaagatg cgagaagccg aacaacagat
     2161 cgagcacctc agaaagcagc tggcagaaat tcagggcgat aaacccatga tcaatgaaga
     2221 agtaagtgcc gaagaagtcg ctgaggtggt gagcaaatgg actggcatac cggtaagtcg
     2281 aatgttgcag agtgaacgtg aaaaactgct tcatctcgaa gaacatctgc ataagagagt
     2341 agtcggacag gacgaagcca tcagtgcggt ggccgatgct atccgacgta gccgagctgg
     2401 actgcaggat cccaaacgac cggttggttc gttcatcttc ctaggaacta ctggagtggg
     2461 taaaaccg
//
LOCUS       DAORPD010000171         2468 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_7641_length_2468_cov_2.464981, whole genome shotgun sequence.
ACCESSION   DAORPD010000171 DAORPD010000000
VERSION     DAORPD010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7641_length_2468_cov_2.464981"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..959)
                     /locus_tag="PK976_02785"
     CDS             complement(<1..959)
                     /locus_tag="PK976_02785"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRC66846.1"
                     /translation="MDQITLSIIIPAYNEEKTIVKVIHQVWETPLQENVSREIIIVDD
                     GSKDATVKVVKEFLDQNSEKNIRLIECGKNRGKGAAIREGIKQACGKWIIIQDADMEY
                     NPRDYNILLQPALEGYADVVYGSRFVGGNPHRILFFWHSIGNKFLTFISNAFTNLNLT
                     DMETGYKLFRADIIKSINLEENRFGFETEVTAKIARIPGIRIYEVGIAYYGRTYAEGK
                     KIKAKDGFRALYTILKYNLQSKNPRRPIALVITLLLLFIVGLKINDYYKDKPFNFTRN
                     DAGEYYQYLPALFIHHDLGFGFLEKTTPYKYEHYYKFSAYRFE"
     gene            complement(972..>2468)
                     /gene="alr"
                     /locus_tag="PK976_02790"
     CDS             complement(972..>2468)
                     /gene="alr"
                     /locus_tag="PK976_02790"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HRC66847.1"
                     /translation="EGINQCTLINDTYNSDINSLEVALDYLNRQAGVRPTAVILSDIL
                     QSGQNPAELYDHVAYLLNLRNVGRVIGIGSEISKFGGLFPMQKNFYVSTQQFIDEFPF
                     SSLQNDVILLKGARSFEFENITRILRRRSHQTVLEINLENLIHNFNFYKQKTKPGTLT
                     LAMVKAFGYGSGMHEIASILQQNGVDCFGVAYPDEGAQLRRAGIQTPIMVMNPENDNL
                     DIITQFHLEPEIYSQTSLTQLAHYLSSRRPDYLPFPIHLKFDTGMHRLGFLPEQLDEA
                     LMFLKQHPNIKVKSVFSHLAASEDPLMDDFTRKQAEIFEEMAQKTIRTLEYPVIRHLV
                     NSAGIIRFPEYHFEMVRIGLGLYGIGHDEKTQRSLQLVSKFSSVISQIKTIRKGEGIG
                     YNLKWRAPADTRIGIVTVGYADGLRRELSSKGFCFHVRGHLAPIIGAISMDMCAIDLT
                     GIAATEGDPVIIFDNADTIHQLSRLLNTIPYEILTSISQRVRRVYLMG"
BASE COUNT          694 a          490 c          516 g          768 t
ORIGIN      
        1 ttttcaaagc gataggccga aaatttataa taatgttcgt acttgtaggg tgtggttttt
       61 tcgaggaatc caaatccaag atcatgatga atgaaaagag cgggtaaata ttgataatat
      121 tcacctgcgt cattcctcgt aaaattgaag ggtttgtctt tataataatc gttgatcttc
      181 aaaccaacga tgaataacaa aagcaaggta ataaccaagg caatggggcg tcgaggattt
      241 ttgctttgga ggttatattt tagaatagtg tataaggccc ggaatccatc ctttgccttt
      301 atttttttcc cctcggcata ggttcggcca taataggcaa tccctacctc ataaattcga
      361 atgccaggta ttcgagcaat ttttgcggta acctccgttt caaacccaaa acgattttct
      421 tctaagttaa tgcttttaat gatatcagct ctgaaaagtt tatagccggt ttccatgtcg
      481 gtgagattta ggttggtaaa agcattcgaa atgaaggtaa ggaatttatt tccgatagaa
      541 tgccagaaaa ataaaatgcg gtgtgggttg cctcccacaa atctcgaccc gtaaactaca
      601 tctgcatatc cttcaagcgc aggttgaagc aagatattat aatcgcgagg attgtattcc
      661 atgtctgcat cctgaataat gatccatttg ccacaggcct gttttatgcc ttcacgaatg
      721 gcagcgcctt tcccacgatt tttaccacat tcaatcagtc ggatattctt ctcactattt
      781 tgatccaaga attcttttac aacttttacc gttgcatctt tacttccatc atccactatg
      841 atgatttcgc ggctaacatt ttcttgtaga ggtgtttccc aaacttgatg aataactttt
      901 actatggttt tttcttcatt ataagcaggg atgataatgc tcagtgtaat ttggtccata
      961 tcaaattaat tttatcccat taaatatact cttctaactc gttgagagat agaagtcaat
     1021 atttcgtagg gaatagtatt tagtaaacgg gaaagttggt gaatcgtatc tgcgttgtcg
     1081 aaaatgataa ccgggtcgcc ttctgttgcg gctatgccgg ttaagtcgat ggcacacata
     1141 tccatagaga tggcaccaat aataggagcc aaatgaccac gaacgtggaa gcagaatcct
     1201 ttggatgata attctcttcg taagccatcg gcataaccca cagtgacaat cccgatgcgg
     1261 gtgtcggctg gtgctctcca tttcagattg taccctattc cttcgccttt tcgaatggtt
     1321 ttaatttgag agataactga agagaatttg cttactaact ggagtgagcg ctgagttttt
     1381 tcatcatggc ctattccata caatccgagc cctatgcgta ccatctcgaa atgatattcc
     1441 ggaaatcgaa taattccggc cgaattgacc aaatgcctga ttacaggata ctccaaagtc
     1501 cttattgttt tttgagccat ttcttcgaaa atttcggctt gttttcgcgt gaagtcatcc
     1561 ataagtgggt cttcgctggc ggcaaggtgc gaaaataccg attttacttt tatgttaggg
     1621 tgctgtttaa gaaacatgag ggcttcgtcg agttgttcag gtaaaaatcc caatctgtgc
     1681 atgcctgtat cgaatttcaa atgaattggg aatggcagat aatcgggtcg tcgagaagat
     1741 aggtaatgtg ccagttgggt caatgaggtc tgactataaa tttctggttc taaatgaaat
     1801 tgggtgataa tatcgagatt atcgttctcg ggattcataa ccattatcgg ggtttgaatg
     1861 ccagctcttc taagttgagc accttcgtcg ggataggcca caccaaaaca gtcaacgcca
     1921 ttttgctgga gaatactggc tatctcgtgc atcccactgc catatccaaa ggctttgacc
     1981 attgccaagg ttaatgtacc cggcttggtt ttctgtttgt aaaaattgaa attgtgaata
     2041 aggttctcca gattgatttc gagcacagtt tgatgagaac gccgacgtaa tattcgggtg
     2101 atgttctcaa actcgaacga tcgtgcaccc ttcaacaaaa tgacatcgtt ttgtaggctc
     2161 gagaagggaa attcatcaat aaactgttga gtggaaacgt aaaaattttt ttgcattggg
     2221 aataaacccc caaatttcga aatctcagaa cctataccta ttacccgtcc cacatttctc
     2281 aaattcagta gataagcaac atggtcgtaa agctcagcag gattttgtcc gctctgtaat
     2341 atgtccgaaa gaataactgc cgtaggacgg acgcctgctt gccggttcaa ataatcgagg
     2401 gcaacttcaa gagaattgat atcgctgttg taagtgtcgt taatcaaggt gcactggttt
     2461 attccttc
//
LOCUS       DAORPD010000172         2467 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_7648_length_2467_cov_2.045191, whole genome shotgun sequence.
ACCESSION   DAORPD010000172 DAORPD010000000
VERSION     DAORPD010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7648_length_2467_cov_2.045191"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            32..1846
                     /locus_tag="PK976_02795"
     CDS             32..1846
                     /locus_tag="PK976_02795"
                     /inference="COORDINATES: protein motif:HMM:NF038324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1998 domain-containing protein"
                     /protein_id="HRC66848.1"
                     /translation="MTQSIRRSQFITTYGPGAILEGPNGPRIIPVIEQSDLFNQQRQV
                     QDFEIREERLPRALLNGSGIFRIPSNAELGEIDSLSLYNTKRFPTWSLCPRHQILYRK
                     TQSDNVACPRCGALSSPAEAWNQSNRQAIRFVTACPDGHLDDVDWVKVAHNRNHNCHP
                     SYLIWRGGGGALKNISIECPLCRDSGNFGFAYTRDWECTGRYPERETTGAGQPIRSGC
                     TKVAKIIQRGAANLRISEIKTALTIPPWDSPIHHLLGMNSIKIRILGIQFQSKSQLMQ
                     SLLPLVQGNLLNQAIYDEINTYTEHEILEAINVIQAANPIQSSRQLRLDEFEALQRAA
                     STGHPPVPSSTPGGPPQFEIIYQQVRTIPGPGNHYLRITPVNRLRVVMVQTGYRRVPG
                     GDPTLSSVVDCRYHDGNRYWYPGVELFGEGIFIDLDPSTNPTSHFPLTGHPANVWMDA
                     WSNPESFSQNLLTPEDRDQLHPVFVWWHTISHRLINALSVDSGYSSAAVRERVYVRID
                     ENSGQALGGVLLYTTQPGGDGTLGGMVALAPAFDRVLNSALRTIDACSNDPLCGEEQF
                     GAGKYNGAACYACSLISETSCEHRNMRLDRNLVLENLP"
     gene            1843..2355
                     /locus_tag="PK976_02800"
     CDS             1843..2355
                     /locus_tag="PK976_02800"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HRC66849.1"
                     /translation="MSSIQNKVNVVVTGTEWMGSGVGSIETVIADLFKTVISELYISA
                     YAISNSSDLFFEWLEKALQRGIKVKILINRLDEQPFEVRGRLKNLLINYPHFFLYEFK
                     STENVDLHAKVIAIDRKKALVGSSNLSKRGLISNFEMGLLAEGPVADTITNTLDKLVL
                     KSLRIMAHDL"
BASE COUNT          730 a          491 c          528 g          718 t
ORIGIN      
        1 ggaagaaaca acaggtttta aggattaatc tatgacccaa tccatccgaa gatcacaatt
       61 cataaccact tatggtccgg gggcaattct tgaagggcca aacggacctc gaattattcc
      121 tgtgatagaa caatctgacc tttttaatca acaaaggcag gtacaggatt ttgagattag
      181 agaggaacgc ttacccaggg ctttgcttaa tggttcaggt atttttagaa tcccatcgaa
      241 tgctgaattg ggtgagattg attctctttc gttatacaac acgaaacgat tccccacatg
      301 gtctttatgc ccccgacatc aaatcttata cagaaaaaca cagagcgaca atgttgcatg
      361 tcccaggtgt ggagcattat catctccggc agaagcatgg aatcagtcaa atcgccaagc
      421 cattcgtttt gttacagctt gtcccgatgg gcatctagat gatgtcgatt gggtgaaagt
      481 tgctcataat agaaaccata actgccatcc ttcgtattta atttggcgag gtggaggtgg
      541 cgcattaaaa aatatctcca tcgaatgtcc tttatgtaga gattccggaa actttggctt
      601 cgcatacacc agagattggg agtgtacggg acggtatccc gaaagagaga caacaggtgc
      661 cggacagcct attcgatctg gttgtacaaa agtcgcgaaa atcatccaaa ggggagctgc
      721 aaatttaaga atatctgaaa taaaaactgc tttaactatt cctccatggg attcgcctat
      781 tcatcatctc ttaggaatga actcaatcaa gattcgaata ttgggtattc aatttcaatc
      841 caaatcacaa ttaatgcagt ctcttttacc tctcgttcag gggaacttgc taaaccaagc
      901 catatatgac gaaattaata cctacacaga gcatgaaatc ctggaagcga ttaacgttat
      961 ccaggccgca aatcctattc aatcttctag acaattaagg ttagatgaat ttgaagctct
     1021 tcaacgagct gcatcgactg ggcatcctcc ggttccatct tcaactcctg gcggtccccc
     1081 tcaatttgaa attatctatc aacaagtcag aacaattcca gggccaggaa atcattatct
     1141 tcgaattact ccggtcaacc gcttacgtgt cgtaatggta cagacaggtt ataggcgagt
     1201 acctggagga gatcctacat tatcatcggt tgtcgattgt agatatcatg atggaaatcg
     1261 ttattggtat ccaggtgtgg aacttttcgg cgaagggatt tttattgatc ttgatccctc
     1321 caccaatccc actagtcatt ttccacttac gggacatccg gcgaacgttt ggatggatgc
     1381 atggtcaaat ccagaatctt tttctcaaaa tttactcact ccagaggata gagatcaatt
     1441 acatcctgta tttgtgtggt ggcatactat ttcacatcga cttatcaatg ccctttctgt
     1501 cgattctggc tattcttctg ctgcggtccg agaaagggtt tatgttcgaa tagatgaaaa
     1561 ttcaggtcag gcattagggg gggttctact ttacacaacc caacccggag gtgatggaac
     1621 actcggtgga atggtcgctc tcgcacctgc ttttgaccgg gttctcaatt cagccttgag
     1681 aacaatcgat gcctgctcga atgatcctct atgcggcgag gagcagtttg gtgcaggtaa
     1741 atataatggt gcagcctgct atgcgtgttc ccttatatct gaaacatcat gtgagcatag
     1801 aaatatgcga ctggatcgga atctggtttt ggaaaatctc ccatgagttc tattcagaac
     1861 aaggtaaatg ttgttgtaac tggaactgag tggatgggta gcggagtcgg ttccattgaa
     1921 actgttatag ctgatctatt taagacagta ataagcgaac tatatatttc agcttatgct
     1981 ataagtaact cctccgatct gttttttgaa tggttagaaa aagcgttaca aagaggtata
     2041 aaggtaaaaa ttttgattaa tcgacttgac gaacagccat tcgaagttag aggtagatta
     2101 aagaatttat taataaatta tccccacttt ttcctatacg agttcaaatc cacagaaaat
     2161 gttgatttac atgcaaaggt aattgcaatt gatcgtaaga aagcattggt cggctcctcg
     2221 aatctttcca aaaggggttt gatatccaat tttgagatgg gtcttttggc cgaaggtcca
     2281 gttgcagata caataacaaa taccttagac aagttggtct taaaatctct gagaattatg
     2341 gcccatgatt tgtgagatta gttgttggtg gcgatcaaat aattaaccag atgaacgagg
     2401 aaatttttat cggtaatatc ctcaatatga ggggcaattt aaatttggga aattctaaac
     2461 caaatta
//
LOCUS       DAORPD010000173         2460 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_7670_length_2460_cov_2.182121, whole genome shotgun sequence.
ACCESSION   DAORPD010000173 DAORPD010000000
VERSION     DAORPD010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7670_length_2460_cov_2.182121"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="PK976_02805"
     CDS             complement(<1..593)
                     /locus_tag="PK976_02805"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRC66850.1"
                     /translation="MLTWQAIALFLGGIFAGFVNTLAGGGSIVSLSLMLLFGLDANVA
                     NGTNRLAIIFQSLTSSETFRVNKILDFKRGKAAVWSALIGSLAGAWVAVDLNKEIIEK
                     IITIVMMAMLLFIFYNPSRWLKGDPERNAKPANLFEIFLFLVIGFYGGFVQVGIGYLL
                     LAGLVLQAGYDLVKANALKGFITFVYSPFVFAIFVWH"
     gene            complement(595..2328)
                     /locus_tag="PK976_02810"
     CDS             complement(595..2328)
                     /locus_tag="PK976_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HRC66851.1"
                     /translation="MHRKSSSFFRRKSVELIIEHAEDKVEGQTSLRRNLGVADLTALG
                     IAAIIGAGIFSTIGTAAAYGGPAVSLLFIFTAIACGFSALCYAEFSSAIPISGSAYTY
                     AYASFGELIAWIIGWDLIMEYAIGNIAVAISWSGYFTGLMEGLGIPIPQFLCIDYLTA
                     SRGFHEIIESGISVEQASRQLQEAFQAWTTAPQIAGIRLIADIPAFGIVVLISWLVYV
                     GIHETKVAGNFMVLLKLITLLVVIGVGAFYVNPDNWSPFAPNGISGVLKGTAGVFFAY
                     IGFDAISTTAEEARNPRQDLPRAMIYALLITTILYILISLILTGMVPYSHLEVSDPLA
                     YVFSFHHINWLSGIISLSAIIAMAGVLLVFQVGQPRIWMSMSRDGLLPKIFSKLHPRH
                     RTPAFATIVAGILVAVPSLFLNLTEVTDLTSIGTLFAFVLVSGGILAVNPHGKKQKKD
                     DINRKFVVPYLNSRIWLLPIWTIMIGSLFYFQPEGFIKSDGLTSLIGNIPILLFAICS
                     IIISIFAILKEWSLIPVLGLLTNLYLMSQLGITNWMRFLIWLVIGLIIYFTYGYRKSR
                     LKGWLKQIKTD"
BASE COUNT          806 a          560 c          512 g          582 t
ORIGIN      
        1 tgatgccata cgaatatggc aaagacaaaa ggtgaataga caaaagtgat gaaacccttc
       61 aaggcattgg cttttaccaa atcataaccc gcttgaagta caagcccggc cagaagtaaa
      121 taaccgattc ctacctgcac aaatccacca taaaatccaa tgaccagaaa caaaaagatt
      181 tcgaacaggt tagccggttt ggcattacgt tcagggtctc ccttcagcca tctcgaagga
      241 ttataaaaaa taaacaacag catggccatc atgacgatgg taattatttt ttcgatgatt
      301 tccttattca ggtcgactgc tacccaggca ccggccagtg agccaatcag ggcactccat
      361 acagcagctt tcccacgttt aaaatccaga attttgttaa cccgaaaagt ctcacttgag
      421 gtaaggcttt gaaaaataat ggccaaacga tttgtcccat tggctacatt ggcatccaaa
      481 ccaaacagga gcatgagcga aaggctcaca atgcttccac ctccagccag agtgtttaca
      541 aaacccgcaa atattccacc caagaacaat gcaattgcct gccaagtcaa catatcagtc
      601 tgtttttatt tgtttgagcc agccttttaa gcggcttttc cgatatccat aagtaaaata
      661 aatgattaac ccgatcacca accatatcaa aaatcgcatc cagttagtta tccccaactg
      721 cgacatcaga tacaggttgg taagaagtcc caatacaggt atcagcgacc attccttcaa
      781 gatggcaaat atcgagataa tgatggaaca gatagcaaat aataaaatgg gtatatttcc
      841 tatcaatgaa gtcaaaccgt cagatttaat aaacccttcg ggttggaaat aaaacaaact
      901 acctatcatg atagtccaga taggtaggag ccagattctg ctattcaaat aaggtacgac
      961 gaattttcgg ttgatgtcgt cttttttttg ttttttgccg tgtggattga cggctaaaat
     1021 tccacccgaa acgaggacga aggcgaagag agtacctata ctggtgagat cggtaacttc
     1081 agtaagattg aggaaaaggg aaggaacggc cacaagaatg cctgcaacga tggtggcaaa
     1141 agccggtgtg cgatgtcggg gatgtagttt cgaaaagatt ttaggaagca gtccatcgcg
     1201 gctcatgctc atccagatgc ggggttgccc tacttggaag accaaaagta cacctgccat
     1261 ggctataata gcactcaaac taataatccc cgaaagccag ttgatgtgat gaaacgaaaa
     1321 aacataggcc agaggatcgg atacctcgag gtgactgtaa ggaaccatac ctgtgagaat
     1381 gaggctgatg aggatgtata ggattgtagt tatcagcaag gcataaatca tggcccgggg
     1441 caaatcttgc cttggattgc gtgcttcctc ggcagtcgtg ctgattgcat cgaaaccaat
     1501 ataggcaaaa aataccccag cagtaccttt tagcactccc gatattccat tgggagcaaa
     1561 aggcgaccag ttgtcgggat ttacataaaa tgctcccact ccgatgacta caagtaaagt
     1621 aatgagtttg agcaatacca tgaaatttcc agccaccttg gtttcgtgaa ttccaacata
     1681 aaccagccag ctaatgagca ccacgatgcc aaaggcagga atatcggcta tcaaccttat
     1741 tcctgctatc tgtggagccg tagtccatgc ctgaaaagct tcctgcaatt gccggctggc
     1801 ttgttcgaca gatattcccg attcgataat ctcatgaaaa cctctggagg cagtcagata
     1861 atcgatacat aaaaattgag ggatgggtat gcccaaaccc tccatcaatc cagtaaaata
     1921 acccgaccag gaaattgcta cggcaatgtt tccgatagca tattccatga tgaggtccca
     1981 gccaataatc caggcgatga gttcaccaaa tgaagcataa gcgtaagtat aagcactgcc
     2041 actaataggt atggccgaag aaaattctgc atagcacagg gccgaaaaac cacaagctat
     2101 agccgtaaag atgaacaaaa gtgaaacagc aggcccccca taagctgctg cagtaccaat
     2161 cgtactgaaa atacccgcac cgataatggc ggctataccc agagcggtaa gatcggcaac
     2221 cccgagattg cgcctcagac tcgtttgtcc ttcaactttg tcttcggcat gttcgataat
     2281 cagttcaacg gatttgcggc ggaaaaaaga ggacgatttt ctatgcatcc ctcattgttt
     2341 gaccgtaaaa ataaaaagaa aaatcttaaa ggatcatatt ttttaatgaa tttggcttgc
     2401 aaattaactg acaacgaacc actaacaact gacaacgaac cactaacaac aaataactta
//
LOCUS       DAORPD010000174         2454 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_7697_length_2454_cov_1.955815, whole genome shotgun sequence.
ACCESSION   DAORPD010000174 DAORPD010000000
VERSION     DAORPD010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7697_length_2454_cov_1.955815"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..199
                     /locus_tag="PK976_02815"
     CDS             2..199
                     /locus_tag="PK976_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HRC66852.1"
                     /translation="MRPYLQRDGGDIEFLDLTDDMQVNVKLVGACGTCPFSILTLKNG
                     IEQAMIKAIPEIKSVEAINIE"
     gene            255..785
                     /locus_tag="PK976_02820"
     CDS             255..785
                     /locus_tag="PK976_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66853.1"
                     /translation="MKKITFWGLSLILLMLMSCKHEKVYNQIVKDETTGKDIYVGYVN
                     VEGLKTEIFKDFYNNGLYNYEPNDSLVQALKPYLDDVTFRVIMGTWCTDTQEEIPRLL
                     HVLYTAEYDPLNPEKFEMICVDRNKKAGNINVKKYNIEKIPTIILYKNGKEIGRIVER
                     FTKRPEADLLDIVKQN"
     gene            792..1646
                     /gene="panC"
                     /locus_tag="PK976_02825"
     CDS             792..1646
                     /gene="panC"
                     /locus_tag="PK976_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005785326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HRC66854.1"
                     /translation="MQLVTTRAEATQFSLSEQSLGKKVGFVPTMGALHQGHLSLVHQS
                     IRQNDSTIVSIFVNPIQFNNADDLKKYPRTLDNDLELLESEGCNMVFAPSVEEMYPQV
                     PTESYSFGLLEQVMEGFYRPGHFNGVAIVVRRLFDILRPHRAYFGEKDYQQLVIIRKM
                     VEQFQLPVETIGCPIVREPDGLAMSSRNRRLAASERAQASFIYQNLVFAQGNAGKLTV
                     KDLKNEIIRRFQNHPSFRLEYFEFSDSSSLQPVDDWNDAANIRAFIAVYLGEVRLIDN
                     IVLKETRV"
     gene            1705..2055
                     /locus_tag="PK976_02830"
     CDS             1705..2055
                     /locus_tag="PK976_02830"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004354715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HRC66855.1"
                     /translation="MMIEILKSKIHRATVTAADLNYIGSITIDEDLMDAANIIENERV
                     FIYNINNGQRFETYVIKGKRGSGVIAINGAAARMVAVGDLVIIVSYATIDFEEAKNFK
                     PTIIFPGKNNQLNW"
     gene            2055..>2454
                     /locus_tag="PK976_02835"
     CDS             2055..>2454
                     /locus_tag="PK976_02835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HRC66856.1"
                     /translation="MRKKLLNIFRFLFFLSIGIFFIWIFVRNLTAQERSEIIASFRRA
                     NYFWIAISICIGLASHASRARRWVILMEPMGYKPKYSNVLMAVFVGYFANLALPRLGE
                     LGRCGVLARYEKIPFNKSLGTVITERTLDLI"
BASE COUNT          749 a          461 c          516 g          728 t
ORIGIN      
        1 gattcgtcct tatctgcagc gcgatggtgg tgatatagaa tttctagacc ttaccgacga
       61 tatgcaggta aatgtaaaac tcgttggagc ttgcggcacc tgccctttca gtatcctgac
      121 tctgaaaaat ggaattgaac aagctatgat aaaggcaatc cccgaaatca aatcggtcga
      181 agccatcaat atagaataac aaataaaaaa ttttttctat cgaaaatcag tgaatcatat
      241 aaaaatccat ttctatgaaa aaaattacat tctggggttt atcacttatt cttctgatgc
      301 tgatgagttg caaacacgaa aaggtatata atcaaatcgt gaaagatgaa actaccggta
      361 aggatattta tgtaggctat gtaaatgttg aaggcttgaa aaccgaaatt ttcaaagatt
      421 tctataataa tggcttgtat aactacgaac ccaatgattc attagtgcaa gccctgaaac
      481 cctatttgga tgatgttact ttcagggtca tcatgggaac ttggtgcacc gatactcagg
      541 aagaaattcc acgcctttta catgttcttt atacagcaga gtatgatccc ttgaatccgg
      601 aaaaattcga aatgatttgt gttgacagga acaaaaaggc tggcaatatc aatgtgaaaa
      661 agtacaatat cgagaagatt cccactatta ttttgtataa aaatggaaaa gaaatcgggc
      721 gcatagtcga aagatttacc aaacgacccg aagccgactt gctcgatatt gtaaaacaga
      781 attaaattga aatgcaatta gttacaactc gggctgaagc cacgcagttt tctctttctg
      841 aacaatcgtt ggggaaaaag gtaggatttg tcccaacgat gggcgctttg caccagggac
      901 atttgtctct ggttcatcaa tcgattcgac aaaacgacag cacaatcgtt agcatttttg
      961 taaatccaat ccaatttaat aatgctgacg acctcaagaa atatcctcgt acgctggata
     1021 atgatctcga acttcttgaa tctgagggat gtaacatggt ttttgcacct tcggtcgaag
     1081 aaatgtatcc ccaagttccg accgaatctt attcgtttgg attgcttgaa caagtgatgg
     1141 aaggttttta taggccaggg cattttaatg gagttgccat tgtagtcagg cgtttattcg
     1201 atattcttcg gcctcatcga gcttatttcg gagaaaagga ttatcagcaa ctggtcatca
     1261 tccgaaagat ggtcgagcaa tttcaattac ccgtcgaaac tataggatgc cccatcgtaa
     1321 gagagcccga tggattggcc atgagttcga gaaatcggcg ccttgcggct tccgaaaggg
     1381 ctcaggcttc atttatatat caaaatctcg tttttgctca gggaaatgct ggcaaattga
     1441 cggtcaaaga tttaaaaaac gaaatcatcc gccggtttca gaatcaccca tctttcaggc
     1501 tcgaatattt cgaattttct gattcatcga gccttcagcc tgtcgatgac tggaatgatg
     1561 ctgctaacat cagggcattt attgcagttt atctcggtga ggttcgtcta attgacaata
     1621 ttgtattaaa agaaactcga gtttgagttg aatagggttg aaaaacctta aaagatccag
     1681 aattaaattt tgcttgttaa tcttatgatg attgaaatat tgaaatcgaa aatccataga
     1741 gctacggtaa cggctgccga cttgaactat attggaagca ttaccattga tgaggatttg
     1801 atggatgcag ccaatattat cgagaatgaa agggttttta tttataatat caataatggc
     1861 caacgtttcg aaacctatgt aataaaaggg aaaagaggca gtggtgtaat agccatcaat
     1921 ggtgctgctg cacgtatggt agcagtgggt gatctggtaa tcatcgtttc ctatgcgact
     1981 atagattttg aagaagcaaa gaatttcaaa cctaccatta tttttcctgg taagaataat
     2041 caattgaatt ggtaatgagg aagaaattac tgaacatttt ccgttttctg tttttcctca
     2101 gtatagggat tttttttatc tggattttcg tacgcaacct tactgctcag gaacgcagcg
     2161 aaattatcgc ttcatttcgt agagccaatt acttctggat tgcaatttct atttgtatag
     2221 gattagccag tcatgcttcg agggctcgac gttgggtcat cctgatggaa cccatgggat
     2281 acaagcccaa atattccaac gttttaatgg cagtctttgt gggctatttt gccaatctgg
     2341 cacttcctcg cttgggtgaa ctcggccgtt gcggtgtgct ggcacgttac gaaaaaatcc
     2401 ctttcaataa atcgctcggc acagtcatca ccgaacgaac cctcgatctg atcg
//
LOCUS       DAORPD010000175         2452 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_7708_length_2452_cov_2.660826, whole genome shotgun sequence.
ACCESSION   DAORPD010000175 DAORPD010000000
VERSION     DAORPD010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7708_length_2452_cov_2.660826"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..538
                     /gene="rnpB"
                     /locus_tag="PK976_02840"
     ncRNA           141..538
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PK976_02840"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            581..913
                     /locus_tag="PK976_02845"
     CDS             581..913
                     /locus_tag="PK976_02845"
                     /inference="COORDINATES: protein motif:HMM:NF015953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HRC66857.1"
                     /translation="MRFFLKWIASAMAIAIIGWILPGIKVESFLTAFIVAGIIAFLNL
                     VLKPILLFLSIPITIFTMGLFILVINAIIVIIASLLVPGFIVNSFGWALLFGLLNSII
                     HFFLDNAD"
     gene            923..1384
                     /locus_tag="PK976_02850"
     CDS             923..1384
                     /locus_tag="PK976_02850"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRC66858.1"
                     /translation="MKHRNITDLEEINQILQAADCCYMGMANVDGSPYVVPMNFGFDN
                     QYIYLHSARTGKKIDILRTNPRVCLAFSAFHQLRYQSEKVACSWSMKYKSVLVFGEVE
                     FIEQKEEKVKMLNCLMKKYTGREFTYNVPALKEVLTYKVSLKKVEARAYRY"
     gene            complement(1442..1526)
                     /locus_tag="PK976_02855"
     tRNA            complement(1442..1526)
                     /locus_tag="PK976_02855"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1490..1492),aa:Leu,seq:taa)
     gene            complement(1570..1642)
                     /locus_tag="PK976_02860"
     tRNA            complement(1570..1642)
                     /locus_tag="PK976_02860"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1607..1609),aa:Gly,seq:gcc)
     gene            complement(1698..>2452)
                     /locus_tag="PK976_02865"
     CDS             complement(1698..>2452)
                     /locus_tag="PK976_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005863767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HRC66859.1"
                     /translation="KSAFLRQTALIVLMAQIGCFVPAQKATIGIVDKIFTRVGASDNI
                     SLGESTFMVEMNETASILNNISDRSLIILDEIGRGTSTYDGISIAWSIVEYLHNHPLF
                     KPKILFATHYHELNEMARIFPRIKNYHIAVREVGNQVVFLRKLMPGGSEHSFGIHVAR
                     MAGMPLKVIERATELLETLENSRNSNSLASSAEKSSKQSSLEGVQLSFFQLDDPLLLK
                     LKDEIHRIDINTITPVEALMKLHQLKKLLGEK"
BASE COUNT          698 a          478 c          523 g          753 t
ORIGIN      
        1 tattccaatg tatattttca gcctgctcca tttgcacgtt ttggaatggg ggccatagaa
       61 gtaatgatta tgcatactcg ggaatagttt ttgattaagt agataaaatc ataattatct
      121 ttgttctggc aaattgaatg atggcagtcc attctatcgc cttggatttc accatccaag
      181 gaggaaagtc gggacaacac agagtagcct gcttcctaac aggaaggtat cgcatagagc
      241 gataacagat agtgccacag aaaagaaccg tcgaagggtt ggttcagcca acaatcgaca
      301 agggtgaaaa cgtgaggtaa gagctcacgc tgcaggatgg cgacatcttg tgggggtaaa
      361 cctcaggcgt tgaaaggcca aataaaccag ggatgctcta tggttcatag agtgagaggg
      421 ttactcgccc aatcctggag ggtaggccga tagaggtaac aggtgactgt tatcccagat
      481 aaatgataga aatcctgaat tgaggataca gaatcccgct tatagggctg ccatcttttc
      541 atttgccttt attgattaga aaacctaact aatttgcaat atgcggtttt tccttaaatg
      601 gatagcctct gcaatggcca tagccattat tggttggata ctcccgggta taaaagtaga
      661 aagtttttta actgctttta ttgtggctgg catcattgcc tttctgaatc ttgttcttaa
      721 acccatccta ctgtttctta gtattcctat tactattttt accatgggat tatttattct
      781 tgtaatcaat gctattatag tgataattgc atctttactt gttcctggct ttatcgttaa
      841 tagctttggg tgggcgctgc tcttcggcct tttaaattcc atcattcatt tttttcttga
      901 taatgctgat taaattcaaa atatgaaaca tcgtaatatt accgatctgg aggagatcaa
      961 ccagatactt caggctgccg attgctgtta tatgggaatg gcgaacgtgg atggaagtcc
     1021 ttatgtggtt cccatgaatt ttggattcga taaccagtat atttacttgc attcagcaag
     1081 aactggtaaa aaaatcgata ttttacgaac caatcccaga gtttgtctgg cttttagcgc
     1141 atttcaccaa ttgaggtatc agagcgaaaa agtagcctgt tcatggagta tgaagtataa
     1201 aagtgttttg gttttcggtg aagtggaatt tatcgagcaa aaagaagaaa aagtgaagat
     1261 gttgaattgc ttgatgaaga aatatactgg cagggaattt acttataatg ttcctgcctt
     1321 gaaagaagta ctcacttaca aagtttcatt gaagaaagtc gaagctcggg cttacaggta
     1381 ttagaaacaa ctcatcacaa aaaacctggt taacatcatg caaggttaag gcttttacta
     1441 gtacccgggg cgggacttga acccgcacga cctcaacggt caaaggattt taagtccttc
     1501 gtgtctacct cttccaccac ccgggccgat aggaaaaccc gaatctgcct gggcagcacc
     1561 cgggctttga gcggaaaacg ggactcggac ccgcgacccc aaccttggca aggttgtgct
     1621 ctaccagctg agctatttcc gcttttcaat tttatgtctg caaaattaat agtattttct
     1681 gtttccttcc aaattcttta tttctctccc aataattttt ttagttgatg caacttcatc
     1741 aaggcttcta ctggagttat tgtattgata tcgatgcgat gaatttcgtc ctttaatttt
     1801 aacaatagag gatcatccaa ttgaaagaaa ctcaattgaa caccttcgag tgaggattgt
     1861 ttcgacgatt tttcggctga agaagccaaa gaattactgt tacgcgaatt ttcgagcgtt
     1921 tcaagcagtt ctgtagccct ttcgattact tttaaaggca tacctgccat acgtgccaca
     1981 tgtattccga aactatgttc acttcctcca ggcatcagtt ttctcagaaa aacaacctga
     2041 ttcccaactt cccttactgc aatgtgataa ttctttatac ggggaaatat tctggccatt
     2101 tcgttcagtt cgtggtaatg tgtggcaaag agaattttcg gtttgaataa aggatgattg
     2161 tggagatatt ctactatgct ccatgcaata gaaataccat cataagtact ggttcctcgt
     2221 ccaatttcat cgagaatgat caagctacgg tcggaaatgt tgttcaaaat gcttgctgtt
     2281 tcgttcattt cgaccataaa ggtagattca ccaagggaaa tattatcgga ggctccgacg
     2341 cgcgtaaaaa tcttatcgac aattcctatg gtagcttttt gggcagggac aaaacatcca
     2401 atctgagcca tcaatacgat gagagcggtt tggcgaagaa aggccgattt tc
//
LOCUS       DAORPD010000176         2451 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_7711_length_2451_cov_2.237062, whole genome shotgun sequence.
ACCESSION   DAORPD010000176 DAORPD010000000
VERSION     DAORPD010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7711_length_2451_cov_2.237062"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..2231)
                     /locus_tag="PK976_02870"
     CDS             complement(99..2231)
                     /locus_tag="PK976_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HRC66860.1"
                     /translation="MPSPLSRLIAKELNLTEQQVENVINLLQEGASIPFIARYRKELT
                     GSLNELSIIAIRDRLKQLTELEKRRDSVIHSVREQGKLTEELEQALRRATTLTEIEDL
                     YLPFRPKRKTRASMAIEKGLEPLAKIILAQKEEDILRLAKRFIDVEKGVENEDEALQG
                     ARDVIAEWVNQDAVVRQRLRRLFRSEAVIASRLVKGKDEEAQKYRSWFHWEEEVRRIP
                     SHRLMAILRGEAEGYLKVEIQPSLAKALEIIERIYMKARNMSSAQVRLALYDAYKRLL
                     QPSLETEIRSELKLKADIEAIGVFSENLRQLLLAPPLRGKNVLAIDPGFRTGCKVVCL
                     DQHGNLLHNETIYPHPPENKVKESMHKIKNLVNAYKIDAIAIGNGTAGRETERFIRHI
                     PFEKDIIAIVVNEAGASIYSVSDIAREEFPDYDVTVRGAVSIGRRLIDPLAELVKIDP
                     KSIGVGQYQHDVDQKMLQESLHDVVESCVNSVGVDVNHASKELLTYISGIGPVLAKNI
                     IEYRKENGPFPSRQQLLEVPRLGAKAFEQSAGFLRIPDAPNPLDTSAVHPESYPMVEA
                     MAAKLGVSVNELIQNKELQQSINIEDFVTEKCGIPTLTDIMKELAKPGRDPRVNFDFF
                     EFDKNVHQIEDLTVGMILPGIVTNITRFGVFVDIGVHQNGLVHISQLSKKYITDPSQV
                     VLLNQKVKVKVLEVDIARSRISLSMKDV"
BASE COUNT          641 a          539 c          495 g          776 t
ORIGIN      
        1 gttgttcgtt cgacgaataa attcgttggc aaaagtaccg aattaaaaac aataggttga
       61 aggataaatt ccaacagagg tggcaatgca gatattgatt atacatcttt catgctcagg
      121 cttatcctac ttcgggctat atcgacctcc agcactttta cctttacttt ttggttgaga
      181 agaaccactt gagaagggtc agtgatatat tttttgctca attggctgat atgtaccagc
      241 ccgttctgat gtactccaat atcaacaaaa acaccgaagc gagtaatatt tgtaacaata
      301 ccaggcaaaa tcattcctac tgtcaagtct tcaatttgat gaacgttttt atcgaattcg
      361 aaaaaatcaa agtttacacg gggatcgcgg ccaggtttag caagttcctt cataatatcg
      421 gtgagagttg ggatgccaca tttttctgtc acaaaatcct caatattgat actttgttga
      481 agttctttat tttggatgag ttcattgaca ctcactccaa gttttgctgc cattgcctcg
      541 accatgggat agctttcggg gtgaacggca ctggtatcga gagggttggg tgcatcggga
      601 atacgtagaa atcctgctga ctgttcgaag gcttttgctc ccaatcttgg cacttcaagc
      661 agttgctgtc gcgagggaaa tggaccattc tcctttctgt attcaataat atttttggct
      721 aaaacgggtc caattcccga aatgtaggtc agcaattcct tacttgcatg gtttacatcg
      781 actccaacgc tgtttacgca gctttctacc acatcatgca aggattcctg aagcattttt
      841 tggtcgacat cgtgttgata ctgtcccact ccaatagatt tgggatcgat cttcaccagt
      901 tctgccagag gatcgataag cctgcggcca atagagactg cgcctctcac ggtgacatca
      961 taatcgggaa attcttcacg ggcaatatca gaaaccgagt aaattgaagc tccggcttca
     1021 ttcaccacaa ttgcgataat atctttttcg aaggggatgt ggcggatgaa tctttcggtt
     1081 tcgcggcccg cagttccatt accaatggct atagcatcaa ttttataggc attcaccaga
     1141 ttcttgatct tgtgcatgct ttcttttacc ttgttttcgg gaggatgagg atagatggtt
     1201 tcgttgtgta aaagattgcc atgctgatcg aggcaaacca ccttacatcc cgttcgaaaa
     1261 cccggatcta ttgccagaac gtttttccct cttaatggag gtgcaagtaa caattggcgt
     1321 aagttttccg aaaaaacccc gatggcttca atatcagcct taagttttaa ttccgagcgg
     1381 atttcggttt cgagtgaagg ttgcaaaaga cgcttataag catcgtataa agccaagcgg
     1441 acctgagccg atgacatgtt tctcgccttc atataaattc tttcgattat ttcgagtgcc
     1501 ttagccagag agggttgaat ctccactttc aggtatcctt cggcttcacc tcgtaaaatc
     1561 gccatcaaac gatgcgaagg aatccttctc acttcttctt cccaatgaaa ccatgaacgg
     1621 tatttttgcg cttcctcgtc cttacctttc accaagcgtg aagcaattac tgcttcactt
     1681 cgaaataacc tgcgcaatct ctgacgtacc actgcatcct gatttaccca ttcggcaatg
     1741 acatcgcgag ctccctgcaa tgcctcatct tcattttcga ctcccttttc aacatcgata
     1801 aacctttttg ccaaccttaa tatatcctct tctttttgtg ccagtattat ttttgccaat
     1861 ggttctaacc ctttttcgat agccatgctg gctctggttt ttcgtttggg acgaaaaggc
     1921 agatataggt cttcgatttc agttaaagtg gtagccctgc gaagtgcttg ttccagctct
     1981 tcagtcagtt tcccttgttc tcttaccgaa tgaattaccg agtcacgtcg tttttcaagt
     2041 tcggtaagct gtttgagcct gtcgcgtatg gcaataatac tcagttcgtt aagactgccc
     2101 gtaagttctt tcctgtaacg cgcaatgaaa ggaatgctgg ctccttcctg aagcaggttg
     2161 atgacgtttt caacctgttg ttcagttaaa ttcaattctt tagcaatcaa tcgagaaagg
     2221 ggtgaaggca tttttcctat tttttacaaa gatagtctcc ttgattttaa cagaaattcg
     2281 ttgacttttt ggagttattt tcaatcgaaa aaaattatta tccaaaagga aataaaccag
     2341 ttttcgagtt ttacacgaca ttcttcaaga tgatgccttc tcgtgctaaa aaattataat
     2401 ggtgaaaaag cctttataaa tgaacgacgt atggagtctc tttgagaagg g
//
LOCUS       DAORPD010000177         2449 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_7721_length_2449_cov_1.942774, whole genome shotgun sequence.
ACCESSION   DAORPD010000177 DAORPD010000000
VERSION     DAORPD010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7721_length_2449_cov_1.942774"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..2073)
                     /locus_tag="PK976_02875"
     CDS             complement(118..2073)
                     /locus_tag="PK976_02875"
                     /inference="COORDINATES: protein motif:HMM:NF037667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HRC66861.1"
                     /translation="MNNKKIHTVCSLIFTFLFAGLNIFEYSFVHTLEAQNLHLMARGK
                     IFPTQNSLDTLETLPPPLPGDKYDIVFMKLELELDPSIHYVKGKAKIYIIPKEDSDSI
                     SLQLHDSLQVEYVNFHGNFLNYSRPGIHALIIKFPEFLHKGELDSLEIMYQGDPPEYL
                     GENAFFTGFHNGKPILWTLSEPYGAFTWFPCKITLDDKIDSTDIVIQCHLDYQAASIG
                     MPVKTDTLEGWVKTSWKHRYPVASYLIGVAVGDYQTYIEEVPTQYGTITTYNYVYSGD
                     ISQQQAATSGLFPVYALYSELFGPYPFIEEKYGHLECGMGGGMEHQTLTFAGQFNHHI
                     LSHELAHSWFGNKITTGSWTDIWLNEGLASYATGLTYQYMFDGFYWKKWKTETIAAVC
                     QQPNGSVIVDDTTFAGRIFDARLSYHKASLVLHMIRFLIGDSAFFRALQNYAANPLLS
                     YGYARTFDLIGQFETSSGINLSEFTAQWLYGEGYPTYTIDWGISGNDTLELSIWQQTS
                     SPSVAFFDMPVEIGIKGAMNDTLIRLNPSFSGEQFQIPLTFTPDSLVFDPNLWLISAN
                     NRVNGIKYEPHETLRVYPNPATSFFYLPVTFSLPIQLTVFDLSGRIIRQCQVIQRSVP
                     VDDLEAGIYIIALSNGKTTAYTKLIKQ"
     gene            complement(2167..>2449)
                     /locus_tag="PK976_02880"
     CDS             complement(2167..>2449)
                     /locus_tag="PK976_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66862.1"
                     /translation="IGRLFINKNMHYFIDGKREVGILYPQFGTLLFDETAAENLIKSS
                     MLYTINFDLLTPPFDEVKVITAGAVIHYAENCRLVIGKRLGFRFQADND"
BASE COUNT          773 a          467 c          515 g          694 t
ORIGIN      
        1 ttcgtctcat ttcaaatgca cttctgcata taatcctcgc ttaacaataa gatttaataa
       61 caaagagctc attgtaggaa taatcgaatc cgaaaaaatc aaggcatatt gttcagatca
      121 ttgctttatc aattttgtat aagctgtggt tttgccattt gatagtgcta tgatataaat
      181 tcccgcttcg aggtcatcta cgggtacaga ccgttgtatc acttggcatt gtcggatgat
      241 tcttccagat aggtcaaaaa cggttagttg gattggcaat gagaatgtaa ctggaagata
      301 gaaaaaagat gtagccggat tgggataaac tctcagggtt tcatgcggtt catactttat
      361 tccattcacc cggttattgg ccgaaatgag ccatagattc ggatcgaaaa cgaggctatc
      421 gggtgtgaat gtgagtggaa tctgaaattg ttcgccggaa aaagaaggat tcaagcgaat
      481 aagtgtgtca ttcattgcgc cttttattcc aatttccacg ggcatgtcga aaaaagcgac
      541 cgagggagaa gaagtttgtt gccagatgga aagctcgagt gtatcgttac ccgaaattcc
      601 ccaatctatg gtatatgtag gatatccttc accatagagc cattgggcag taaattcgga
      661 aagattgata cccgaactgg tttcgaattg tccaattaga tcaaaagttc gggcataacc
      721 ataagaaagc aaggggttag ctgcataatt ttgcaatgcc cgaaaaaaag cgctatcgcc
      781 aattaaaaat cgaatcatgt gaagcaccaa tgaggctttg tgatacgaca ggcgcgcatc
      841 gaaaatccgg ccagcgaagg tagtatcatc tacgatgaca gaaccgttag gttgctggca
      901 aacggcagca atcgtctcgg ttttccattt cttccagtaa aatccatcga acatgtattg
      961 ataagtcaag cccgtggcat acgaagccaa gccctcattt aaccaaatat cagtccagga
     1021 acccgttgtt atcttgttcc cgaaccatga atgtgccagt tcgtgcgata aaatatgatg
     1081 attgaattga ccggcaaacg tcaaagtttg atgttccata cctccaccca tgccacattc
     1141 cagatgtcca tacttttctt cgatgaaagg ataaggacca aataattctg aataaagcgc
     1201 atacaccggg aaaagccctg acgtagcagc ttgttgctgg cttatatctc cggaataaac
     1261 gtagttgtaa gttgttatgg ttccatattg agtagggact tcctctatat aagtttgata
     1321 atcacccacg gcaacaccta taagataaga ggcaactggg taacgatgtt tccatgacgt
     1381 ttttacccat ccttcgaggg tatcggtttt gacgggcatt ccaatgcttg ctgcctgata
     1441 atctaaatgg cattgaatca caatatccgt agaatcgatt ttatcatcga gggtaatttt
     1501 gcatgggaac caagtaaatg ctccataggg ttcggatagt gtccacaaaa ttggcttgcc
     1561 attatgaaaa cctgtaaaaa acgcattttc tccgagatat tcaggtggat caccctgata
     1621 catgatttcc aagctatcta attctccttt atgcagaaat tctggaaatt tgatgataag
     1681 cgcatgtatc ccaggtctcg aataattcaa aaaatttcca tgaaaattaa catattccac
     1741 ttgaagcgaa tcgtgtaatt gcaaagaaat gctatcgcta tcttcttttg gaataatgta
     1801 aattttggct ttccctttta cgtaatgaat cgaggggtct aattcgagtt ccaacttcat
     1861 aaaaacaatg tcgtatttat cgccgggcaa tggtggcggc aatgtttcaa gtgtatcgag
     1921 cgagttttga gtaggaaata tctttcccct ggccataaga tgaaggtttt gagcttcaag
     1981 agtatgaaca aaagaatatt cgaaaatatt caggccagca aagaggaatg taaaaatcaa
     2041 tgagcagacg gtatgaattt ttttattgtt catggcttac aaatagaaga taaaatcttc
     2101 cttaaaaata aatcatggga cgagaaaacg aaaaattgaa taaaaaatca aagagatcgg
     2161 aaaacattag tcattatcgg cctggaatct aaaccccaaa cgtttgccga tgacgaggcg
     2221 gcagttttcg gcataatgaa tgacagctcc ggctgtaatc acttttactt cgtcgaaggg
     2281 tggagtcaac agatcgaaat taatggtata aagcatgctc gatttgatta ggttttcagc
     2341 cgcagtttca tcaaacaaaa gggttccgaa ttgtggataa agaataccga cttctctttt
     2401 accatcaata aaataatgca tgttcttatt gataaaaagc cgtccgata
//
LOCUS       DAORPD010000178         2445 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_7741_length_2445_cov_2.129707, whole genome shotgun sequence.
ACCESSION   DAORPD010000178 DAORPD010000000
VERSION     DAORPD010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7741_length_2445_cov_2.129707"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..728
                     /locus_tag="PK976_02885"
     CDS             <1..728
                     /locus_tag="PK976_02885"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HRC66863.1"
                     /translation="LCTNCQNMDESEVLILRSKMENLTEDAAIKEVAEWFPSRVCFAS
                     SLSAEDQVILHLIATQNLPIRIFTLETGRLFEETYDLLEKSRLRYKINIEVIFPDYKR
                     VEKLVNEKGINLFYYSVENRKMCCSVRKVEPLRRALEGMKLWITGLRRQQSQTREHTQ
                     LLEWDEVNGLLKFNPLIAWTDEKLWNFIHDHQIPYNPLHDKGYPSIGCEPCTHAIQPG
                     EDVRAGRWWWEAQGGRECGIHRS"
     gene            complement(1143..>2445)
                     /locus_tag="PK976_02890"
     CDS             complement(1143..>2445)
                     /locus_tag="PK976_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66864.1"
                     /translation="WLGKYIKPVAAAILAILIALPVPYVMAKDGWDDHNRSHKTAARD
                     FAFAYLDSTQPQSILFTFGDNDTFPLWYAQEVENHRTDVRVVNYMLASSDWYIHQMAR
                     KVYDSEPLPFTLKPEQYDKGNNDYVMVVDLNKSGQPILLRDIMRFIADERNKRDFGDG
                     QRLSFIPSKTIRIPVDTAAVKASGLIPNEMKNRVLPYLDFTIRRNYIVKNDLMLLDIL
                     TTNDWKRPIYLTSPSGIGGAINLDEYCHLEGMVYKFLPVKATDYIRGLGGVSSDTCYD
                     VLVNRIKQWGNLNDPRVTVDRESFRNAAIPRQNYMRVAQSMLNKGKNEEAEKALDLSL
                     QYFPTSKIFPDKYMLSYVDLYYAAKATEKANNMALQLANIFSQDLNFYLSLEPKYSSQ
                     YEEEMSENAYLIQRLSQVASQNGQDSTAKAIESMINLKLSQ"
BASE COUNT          723 a          449 c          531 g          742 t
ORIGIN      
        1 atctttgcac aaactgccaa aatatggacg aaagcgaagt attgattttg agaagcaaga
       61 tggagaattt aactgaagat gcggctataa aggaagttgc ggagtggttt ccttcgcgag
      121 tatgttttgc cagcagcctg agtgccgagg atcaggtgat tttgcatttg attgccactc
      181 aaaatcttcc gatacgtatt tttacgctcg agacggggcg gcttttcgaa gaaacttatg
      241 atttactcga aaaatcccgt ttaaggtaca aaatcaatat cgaagtaatt ttccctgatt
      301 ataagcgagt ggagaagttg gtgaatgaaa aggggattaa tcttttctat tatagtgttg
      361 aaaaccgtaa aatgtgctgt agcgttcgaa aggttgaacc tttaaggcgt gctctcgaag
      421 gaatgaagtt atggattacc gggctcagga gacaacagtc gcaaacacga gaacacacac
      481 agttattgga atgggatgag gtaaatggtt tgttaaaatt caatcctctc atagcatgga
      541 ctgacgaaaa gttatggaat ttcattcatg atcatcagat tccttataat cccttgcacg
      601 ataagggata tccgagcata ggttgtgaac cttgtactca tgccatacaa cccggtgaag
      661 atgtcagagc tgggcgctgg tggtgggaag ctcaaggagg acgcgaatgt ggaattcacc
      721 gaagttaaaa catgttgcaa ccaatatttt ttaacggagg gtcaagcact aagaaattaa
      781 aaataaagaa gctgatttta attttaaatg gcaatatttt cagctaagaa ttttttcaaa
      841 ttcattatga gtccctataa agattattat tatattatgt cggctcttgt gctacgagaa
      901 tttttatatc aggtttaaat ctcatagaga attcaatgac ccacaatatc ctgaatatcc
      961 tactgaaaaa aatccgatat actggatctg atcactaaaa catcgtctaa cgaaaacagc
     1021 attgacttga attatttctg tggctcgaga accaattcaa aatcagccca tattcatcat
     1081 ccaaactaga ttggcatcga taagtcagca cttgccataa ggactacaat cgagaaatgg
     1141 atttattggg atagcttaag gttaatcatc gattcgatgg ctttagccgt actatcctgt
     1201 ccattttgag aggctacctg acttaaacgt tgtatcagat aggcattttc tgacatttct
     1261 tcttcgtatt gagatgaata tttgggttcg agtgaaaggt agaaattaag atcctgtgaa
     1321 aagatattag ccaactgcag tgccatgttg ttcgcttttt cggtagcttt tgctgcgtaa
     1381 tataaatcaa catatgagag catatattta tcggggaata tttttgaggt tgggaaatat
     1441 tgtaacgaca aatcgagtgc tttctctgcc tcttcgtttt tgcctttatt tagcatagac
     1501 tgggcaacac gcatatagtt ttgtcttggt attgcagcgt tgcgaaaact ttctctgtca
     1561 accgtcactc tgggatcatt gagatttccc cactgtttaa tacgattgac tagaacgtca
     1621 tagcacgtat cggacgaaac tcctcctaat cctctgatat agtcagtggc tttaacaggc
     1681 aagaatttat aaaccattcc ctcaagatga cagtattcat ccaaattaat agctcctcca
     1741 attcctgaag gactcgttaa atatataggt cttttccaat cgttggtggt taagatatca
     1801 agcaacatta aatcattttt aacgatataa tttctgcgaa tagtgaagtc gagatagggt
     1861 aaaactctat ttttcatttc gtttgggatg aggccacttg cttttactgc agctgtatcc
     1921 acagggatac gaatggtctt ggaaggtatg aagctaagcc tttggccatc gccaaaatca
     1981 cgcttgtttc tttcatcggc aataaatctc atgatgtccc tcagcagaat gggttgtccc
     2041 gatttgttca ggtcaaccac catcacgtaa tcgttattgc ccttatcgta ttgttcgggt
     2101 ttcagggtaa atggaagggg ttcagagtca tagacttttc gggccatttg atggatgtac
     2161 cagtcgcttg aagccagcat gtagtttact actcttacgt cggtgcgatg attttctacc
     2221 tcttgggcat accacaaggg gaaagtatca ttatcgccga aggtaaaaag tatgctttga
     2281 ggctgggttg aatcgagata agcaaaggcg aaatcacgag ctgcagtttt atgagaacga
     2341 ttgtggtcgt cccatccatc ttttgccatg acatagggaa cgggcaatgc aatcaaaata
     2401 gctaagatgg ctgctgcaac gggtttgatg tatttcccga gccaa
//
LOCUS       DAORPD010000179         2439 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_7764_length_2439_cov_2.069211, whole genome shotgun sequence.
ACCESSION   DAORPD010000179 DAORPD010000000
VERSION     DAORPD010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7764_length_2439_cov_2.069211"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2380)
                     /gene="sprA"
                     /locus_tag="PK976_02895"
     CDS             complement(<1..2380)
                     /gene="sprA"
                     /locus_tag="PK976_02895"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HRC66865.1"
                     /translation="MVVGENYITDIYEATNIPLENGERGSVKWYQFKIPINDPQLIIG
                     NIQDFKSIRFMRMFFKNWQKPITMRFATFELVRGDWRRYRYDLLSPGEYIPNDEQSMT
                     KFEISTVNVEENGSKYPVPYVIPPGIEREINYGTTNFTRLNEQAMVLRVKELQDGDAR
                     AAYKTTGFDFRQYHRLKMFVHAEDLDALNPNNYGDLTVFLRLGSDFTQNYYEYEIPLT
                     FTPWGTAKTNPALIWPDENSFDIDLDKLVQMKQERNIQMRDGETPISLTVPYIAYDGK
                     NKVTIVGTPSISDVRAIMIGVRNPKKKSFTDADDGQPKSTEIWVNELRLTDFNDQSGW
                     AATGRLTVNLADLGNISVNLAHSTPGFGSIEKKVNERQKETITSYDFATNLQLGKFLP
                     EKLGLRFPMHVDYSTTVASPQYNPLDPDIKLKDELDSYDVSSQKDSIRKLTEDYTQRK
                     NINFMNVRKERVGTKRQPMPWDVENFDFTYSYSELNRRNIDIEYDNKKIYRGGIGYTY
                     MTRPKVYRPFEKIGIVSRSKDLQLIKDFNFSLLPRMFSFRTDINREYTERKLRNKSQA
                     LILIEPTYVKSYVWTRMYDYKHDLTQGLKLEYNANANALIDELPGSVDKDDYGYTVKE
                     KKDRIHEELMGFGTMNQFNQVLNLNYTIPINKIRAFNWMTATARYQGTYNWTASPKSI
                     QEVMGNKAENSNTIQLNSNLRFTSLYNKVPYFKKVLSTTQTQRGGPQPARPGQKPQPT
                     LQKPSREDTAKVAKPKKDYFKIISESMVKIVLGFKDGSLSYSETNGIFLPGFK"
BASE COUNT          617 a          499 c          511 g          812 t
ORIGIN      
        1 gtttaaaccc cggtagaaaa atgccattag tttcggaata cgaaagtgat ccatccttga
       61 aaccgagcac aatctttacc atcgattcag aaatgatctt gaagtaatct ttctttggtt
      121 tagcgacctt tgcggtatct tcacgggagg gtttttgtag tgtaggctga ggtttctgcc
      181 ccggtcgcgc tggctgagga ccacctcttt gggtttgagt cgtactcaaa accttcttaa
      241 aataagggac tttgttgtaa aggctcgtaa acctcaggtt gctgttcaat tgaatagtgt
      301 tggaattctc ggctttattt cccatgactt cctgaatgct ttttggcgaa gctgtccagt
      361 tatatgttcc ctgataccgt gccgtagcag tcatccagtt aaaagcccga atcttattga
      421 tgggaatggt ataattaaga tttaatacct gattaaattg attcatggtt ccaaaaccca
      481 ttaattcttc atgtatgcgg tcctttttct ccttcacggt atagccatag tcgtccttgt
      541 ccacacttcc tggcagttca tcgatcaaag cattggcatt ggcattatat tcgagtttca
      601 aaccttgtgt aaggtcgtgt ttataatcgt acatacgagt ccaaacgtac gattttacat
      661 aggtaggctc gatcaatatc aaagcctgac tcttgttgcg aagtttgcgt tcggtgtatt
      721 cacggttgat atcggttcgg aaactgaaca ttcgtggcaa aagcgaaaag ttaaaatctt
      781 ttatcaattg aaggtctttg ctgcggctaa cgatgccaat tttttcgaaa ggtcggtaaa
      841 cctttggtct ggtcatatag gtgtatccta ttcctcctct gtagattttc ttgttgtcat
      901 attctatatc gatgttgcgc cggtttagtt cgctgtaaga atatgtaaaa tcgaaattct
      961 ccacatccca aggcatgggc tgacgttttg ttcctacacg ttcctttcga acgttcataa
     1021 agttgatatt ctttcgctgg gtataatctt ccgttagctt tcttatcgag tctttctgcg
     1081 atgaaacgtc ataactgtcg agttcatcct ttagcttgat atccggatcg agaggattat
     1141 attgtggact tgccacagtg gtggaataat cgacatgcat gggaaatcgt aaacctaatt
     1201 tttcgggtaa gaatttcccc aattggagat tagtggcaaa atcatacgat gtaatggttt
     1261 ctttctgtcg ttcatttacc tttttttcga tacttccaaa acccggtgtg ctgtgggcta
     1321 aatttaccga aatattaccg aggtcggcca gattaacggt taggcggcca gtagctgccc
     1381 atcccgattg atcattaaaa tcggtgagcc ttaattcgtt tacccatatc tcggtactct
     1441 tgggctggcc atcatcagca tcagtgaagg attttttctt gggattgcgc actccaatca
     1501 tgatggctct cacatcgcta atacttggtg taccaactat ggttacctta tttttcccat
     1561 cataagcaat ataaggaacg gtcaaagata taggagtttc tccatccctc atttgtatat
     1621 tccgttcttg cttcatttgt accagtttat cgagatcgat gtcgaaactg ttttcgtcgg
     1681 gccatatcaa agcgggattg gtttttgccg ttccccatgg cgtaaaggtc aaagggattt
     1741 cgtattcgta atagttttgg gtaaaatcgg agcccaaacg cagaaaaact gtcaaatcgc
     1801 catagttatt aggatttaat gcatcgaggt cttcggcatg gacaaacatt ttcagcctgt
     1861 gatattgtct gaaatcgaag ccggttgttt tataagcagc tcttgcatca ccatcctgaa
     1921 gttcctttac tctcagaacc attgcttgtt cgttgaggcg ggtgaaattg gtagtcccat
     1981 aattaatttc tctttcgatg cctggtggga ttacataagg aaccggatat ttacttccat
     2041 tttcttcgac atttaccgta gagatttcaa atttggtcat gctttgttca tcgttaggga
     2101 tatattcgcc aggtgaaagc aaatcatatc gataacgacg ccaatcgccc cttacgagtt
     2161 caaaagtcgc aaaacgcata gtgatgggtt tttgccagtt tttgaaaaac atcctcatga
     2221 agcggataga tttgaaatcc tgaatgtttc caatgatgag ttgagggtca ttaataggga
     2281 tcttaaattg ataccatttt accgagcccc gctctccatt ttcgagtgga atattcgtgg
     2341 cttcgtagat atcggtaata tagttctccc caacgaccat tttatcaggt cttaaatcta
     2401 tccgatattg aaaatatctt tcgtcttctg acagggtat
//
LOCUS       DAORPD010000180         2425 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_7835_length_2425_cov_2.104219, whole genome shotgun sequence.
ACCESSION   DAORPD010000180 DAORPD010000000
VERSION     DAORPD010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7835_length_2425_cov_2.104219"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(223..1446)
                     /gene="rocD"
                     /locus_tag="PK976_02900"
     CDS             complement(223..1446)
                     /gene="rocD"
                     /locus_tag="PK976_02900"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005846053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HRC66866.1"
                     /translation="MSNQLTSQQLIQLEENYGAHNYHPLPVVLAKGQGPYVWDIEGKK
                     YYDFLSAYSAVNQGHCHQKIVNALIEQAQKLTLTSRAFFNDSLGVYEKFITEYFGYDK
                     VLPMNTGVEADETALKLCRKWAYQKKGIPDGEAKIIVCEGNFHGRTITVISMSTDPDA
                     YREYGPYTPGFIKIPYNDLEALAKELEDPTVAGFLVEPIQGEAGVYVPDEGYLAKAYA
                     MCKEKNVLFMADEVQTGLGRTGKLLACDYENVRPDILILGKALSGGVMPISAVLADDH
                     IMLCIKPGEHGSTFGGNPLAARVAIAALEVIKDEHLADNAARLGEIFRNEMRTIKSEM
                     VELVRGKGLLNAIVIHPRNGKEAWDVCLKMAENGVLAKPTHQHIIRFAPPLVITEEQL
                     REAIEMIKKSILSFH"
     gene            1673..2119
                     /gene="tsaE"
                     /locus_tag="PK976_02905"
     CDS             1673..2119
                     /gene="tsaE"
                     /locus_tag="PK976_02905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HRC66867.1"
                     /translation="MMIVFRTSHLGELDHTASLMLSAFPNHRIFAVEGPIGSGKTTLI
                     KAFCQTLQIYDKVSSPTFSIINVYESPQGLVYHFDLYRLNDMAEFIATGAMEHLESGA
                     YCFIEWPNKALEILPHETIIIKINVEDDGARIYEMEPLPERTHKLR"
BASE COUNT          720 a          536 c          452 g          717 t
ORIGIN      
        1 taatcgaatg gagagacccg accatacata ataatttttt gatggcagtc caatggcacc
       61 ccgaacgctt gactgttacc cccgaaatga gccaaccgct tggcatggct tttattcgcc
      121 aagccaagct atatcatgaa tcaaaaaaat caaaagatta aaaaaacaaa cagtcgcctt
      181 ataaagcgcg actgtttttt aaaattcatc gagttgagcc gatcaatgaa aactgaggat
      241 tgactttttg atcatttcga tggcttctcg cagttgttct tctgtaatga ccaatggagg
      301 agcaaaacgg atgatatgtt gatgtgttgg tttggccaga acaccgtttt cggccatttt
      361 caaacaaaca tcccatgctt cttttccatt tcttggatgg atgactatgg cattcagcaa
      421 gcctttccct ctcacaagtt ctaccatttc gctcttaatg gttctcatct catttctgaa
      481 gatttcacca agccgtgcag cattatcggc cagatgttcg tccttaatga cttctaaggc
      541 agcaatagct actcgagcgg ccaaaggatt tcctccaaaa gtcgaaccat gctctccggg
      601 ctttatgcac agcattatgt gatcatcggc tagaaccgcc gaaattggca tcactccgcc
      661 actgagggct ttcccaagaa tcaaaatatc gggtcgtaca ttttcatagt cgcaagcaag
      721 tagcttcccc gtacgtccta atcctgtctg tacctcatcg gccataaaca gtacattctt
      781 ttctttacac atagcataag cttttgctaa atacccttca tcaggaacat atacgcctgc
      841 ttcgccctga atgggttcaa ccagaaaccc tgccactgtc ggatcttcca attcctttgc
      901 caaagcctct aaatcgttat aaggaatttt aatgaaaccg ggagtataag gaccgtattc
      961 acggtaagca tcagggtcgg tcgacatcga aatcaccgtg atggtgcgtc catgaaaatt
     1021 accctcgcat acgataatct ttgcttcgcc atcgggtatg ccttttttct gataggccca
     1081 tttgcggcat aatttcaggg cagtttcgtc ggcttcaaca cctgtattca tgggtaaaac
     1141 tttatcatac ccaaaatact cggttatgaa tttttcatac acccctaagg aatcattgaa
     1201 gaatgcacgt gaagtgaggg tgagtttttg tgcttgctca attagtgcat tcacaatctt
     1261 ttgatgacaa tgtccttgat ttacagccga gtaagccgaa agaaaatcat aatacttctt
     1321 cccttcgatg tcccaaacat acggtccctg accctttgcc agcactaccg gcaatggatg
     1381 gtaattgtgt gctccataat tttcttctaa ttgaatgagt tgttgagatg tgagttgatt
     1441 tgacataata taaaatttta taaaacgtta gtattcctca atcctattca aacctagcaa
     1501 aattagctat ttagtggaat ataaaaaaat gcagttacca gatatctact ctgtctattg
     1561 gataaaatgc tgctaatctg ctaaatgtgc gccaaataaa atatttttgc aaattcatga
     1621 ttcaaaaacg atactaagca gttttatttc attttgaaat cactaaaatc agatgatgat
     1681 tgtttttaga acttcacatt taggcgaatt agaccatacg gccagcctga tgctctcggc
     1741 ttttcccaac cataggattt ttgccgtgga agggcctatc ggtagcggga aaactacctt
     1801 aataaaggct ttttgccaga cattacagat ttacgataaa gtatcaagcc ccaccttttc
     1861 tatcatcaat gtatatgagt cacctcaggg gttagtatat cacttcgatc tttaccgcct
     1921 gaacgacatg gcagagttta tagctactgg tgccatggaa cacctcgaaa gtggagctta
     1981 ctgctttatt gaatggccta ataaggctct ggagattctt ccccatgaga ccatcatcat
     2041 aaaaataaat gtggaagacg atggcgcccg aatttatgaa atggaacccc tcccagaaag
     2101 gacccacaaa cttcggtaaa acctatatga gttatgattg tatcaatata aggggttaag
     2161 ccacgttcaa gaaatctcaa aaaattgaat tatactttgg taagcgataa gatgatagca
     2221 ggagctacag caattacatc gttggatatc aattatcttt atttccactt tacctttaca
     2281 aagagcaagc tccccaaaaa tcgcattcct cgtcgatgat gattttgttt gaacctaaaa
     2341 tggaatatga aagcagtaaa acgcctaatc tcgacgaaaa ggttaccgta tcttttaaaa
     2401 atttatggtc gtgaacttac gcccg
//
LOCUS       DAORPD010000181         2420 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_7857_length_2420_cov_2.475264, whole genome shotgun sequence.
ACCESSION   DAORPD010000181 DAORPD010000000
VERSION     DAORPD010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7857_length_2420_cov_2.475264"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            400..1362
                     /locus_tag="PK976_02910"
     CDS             400..1362
                     /locus_tag="PK976_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HRC66868.1"
                     /translation="MEKENIRKIAEQTLHEEALAIQGLKQHIDDEFVHAIEVILEASG
                     RVIITGIGKSSDIGRKFTATLNSTGTPAFFLHAADAIHGDLGMVRPDDIVVCLSKSGD
                     TPEIKVLLPLIKANGNTLIGIVGNPQSYLGLQSDIVLNVHVEHEACPNNLVPTASTAA
                     QLAMCDALAVALLTARGFTIEDFARLHPGGALGKKLYLRVSDLYLRNEKPQVETHTPI
                     TQVIFEISSKRLGCTAVLDQDRLIGIITDGDLRRMLQKNIELKNIVASDVMTTSPKTI
                     LPSSLVADALDVMRSNNITQLLVTENGIYLGVIHLHDILKEGVV"
     gene            1384..1740
                     /locus_tag="PK976_02915"
     CDS             1384..1740
                     /locus_tag="PK976_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HRC66869.1"
                     /translation="MKDNVDDFKEYRQRMNEKILAAPNNIVLKRIYSVDAMAYKDGAL
                     NAKTKEMLGLAISMVLRCDDCIRYHLIQCHQLGVTTEELLELFGIVNLIGGTIVIPHT
                     RRAFEFWEELNPQNHL"
     gene            1737..>2420
                     /gene="lptB"
                     /locus_tag="PK976_02920"
     CDS             1737..>2420
                     /gene="lptB"
                     /locus_tag="PK976_02920"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HRC66870.1"
                     /translation="MKLHTQGLYKKYRNRMVVKDVSVEVNQKEIVGLLGPNGAGKTTT
                     FYIIVGLIKPFAGKVFIDDQEITSFPMYKRAQLGVGYLAQEASVFRNLSVEDNIKAVL
                     EFSHFSKNEQKKRLEELLTEFGLNHIRKSKGIQLSGGERRRTEIARALAVDPKFILLD
                     EPFAGIDPIAVEDLQNIVAHLKDKNIGVLITDHNVHETLSITDRAYLLYEGSVMKSGT
                     SEELANDEQV"
BASE COUNT          749 a          465 c          517 g          689 t
ORIGIN      
        1 cttttgggga tacattttat ttctttccaa cgacaaaaat gcatcttcat tctgtattgt
       61 aaaaataata taaaaatctg aaacttttaa attttcatgt aaaaattttt ccatttttca
      121 ttctgacaaa aggtcaataa ggttttcttc cgacgtttat ttcactcgac gtattacaat
      181 acattgaaat tcaatctatt aaaataattt tttctgcaac ttaacttcag ggggtgatga
      241 agaaagtgaa gggtttgcaa tgactttgag gtaagtatga tattctataa tatgcgttgg
      301 atcctcattt cctaagcggt ttattggatc atcatctctc gattagaacg gagaagtaag
      361 gtatattctt atctttgcaa cattaaaaat tgctaaaggg tggaaaaaga gaacatcagg
      421 aaaatagctg agcagacctt gcatgaagaa gcgctagcca ttcagggttt aaagcaacac
      481 attgatgatg aatttgttca tgctatagag gtgattctgg aagcaagtgg tagagttata
      541 ataaccggga tagggaaaag ctcggatata ggtcggaaat tcacagccac cttgaattcg
      601 acgggtaccc ctgcattttt tcttcatgct gccgatgcca ttcatggtga tcttggaatg
      661 gttcgcccag acgatatagt agtttgcctc tcgaaaagtg gcgatactcc cgaaataaaa
      721 gtcttattgc cactaattaa ggccaatgga aatactttga tcggaatcgt aggaaatcct
      781 caatcctatt taggtctcca atccgacatc gttctcaatg tacatgttga gcatgaagcc
      841 tgtccgaaca acctcgttcc tacagccagt actgcggctc agctggccat gtgcgatgca
      901 ttggcagtag cattgctgac tgccagagga tttactatcg aggattttgc tcgactacat
      961 cctggaggtg cattagggaa aaaattatac ctgagggttt ctgatctcta tctaaggaac
     1021 gaaaaacctc aggttgaaac tcatacgccc attactcaag taattttcga aatctcttcc
     1081 aaacgcctcg gctgtaccgc agttcttgac caagatcgat tgataggcat aattaccgat
     1141 ggcgacctta ggcgaatgct tcaaaaaaat atagaactga aaaatattgt ggcatctgat
     1201 gttatgacca cttcgcctaa aacaatccta ccctcgagtc tggtggctga tgccctggat
     1261 gtgatgcgct ccaataacat tactcaattg ctggttaccg aaaatggtat ttatcttggc
     1321 gtaattcact tacatgatat tttgaaagaa ggggtggttt aatgtattga ttatttgcta
     1381 aaaatgaaag ataacgtgga tgatttcaag gaatatcgcc agagaatgaa tgaaaagatt
     1441 ctggctgcac ccaataatat agttttaaaa cgaatttaca gcgtcgatgc catggcttat
     1501 aaggatgggg cattgaatgc aaagaccaaa gaaatgctgg gcttagctat aagtatggtg
     1561 cttcgatgtg acgattgcat tcgctaccat ctcatccaat gccatcaact cggggttaca
     1621 accgaagaat tactcgaact cttcggcatc gtcaatttaa taggaggcac catcgttata
     1681 ccacatacac gaagagcctt cgaattttgg gaagaactta accctcagaa tcatttatga
     1741 aactgcacac tcagggttta tacaaaaagt atcgcaatcg tatggttgtg aaagatgtgt
     1801 cggttgaagt gaaccagaaa gagatagtgg gattgcttgg gcccaatggg gcagggaaaa
     1861 cgacgacttt ctacatcatt gtgggtttaa taaaaccctt tgcaggcaaa gtattcatcg
     1921 atgatcagga gattacatca ttccctatgt ataaacgagc tcagcttgga gttgggtatc
     1981 tggctcagga agcatcggta tttcgtaact tgagtgtaga agataatata aaggctgtgt
     2041 tggaattcag tcattttagc aaaaatgaac aaaaaaaacg cttggaagaa ttgcttaccg
     2101 agttcgggct caatcatatt cgcaaaagca agggtattca attatcagga ggtgaacgcc
     2161 gaagaaccga gattgctcgt gctttggcag tcgaccccaa gtttattctt ttagacgaac
     2221 ccttcgctgg tatcgaccct atcgctgtcg aagaccttca aaatattgtg gctcacctaa
     2281 aggataaaaa tattggcgta ctaattaccg accacaatgt tcatgaaacc ctcagcataa
     2341 ccgaccgcgc ctatctgctt tacgaaggaa gtgtcatgaa atcgggaaca tcagaagaac
     2401 ttgccaatga cgaacaggtg
//
LOCUS       DAORPD010000182         2419 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_7866_length_2419_cov_2.489002, whole genome shotgun sequence.
ACCESSION   DAORPD010000182 DAORPD010000000
VERSION     DAORPD010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7866_length_2419_cov_2.489002"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1228)
                     /locus_tag="PK976_02925"
     CDS             complement(<1..1228)
                     /locus_tag="PK976_02925"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRC66871.1"
                     /translation="MESFKKKIVWRLWFIYWLMFLLGMVILGKVIYIQAVKGKEYREM
                     VKQSTFKFDEIEAIRGNILAADGSLLATTVPIFDVRMDVANPNISKEFFNKKVDSLAL
                     GLSKLFPNKSRNEWKSELKEARKQGNRYYLIKRNVDYSTLKRIKTLPILCEGKNKGGL
                     ILIQKNIRKMPYGMLARRTIGYENKQEGILVGLEGAYDEELKGINGSMLMQKISGGEW
                     IPVMGAQRIEPRNGKDIVTTIDIKLQDVAEQALLEQLTENDAYQGCVVLMEVATGDIK
                     VIANLRKIKDGSYDETYNYAIAESIEPGSIFKLPSLVAVMEYAGYNIKDSVSYQGSIS
                     WSRNAVMKDDHPLNRTYITLEDAFAHSSNVGVSKIVYKAFGKKPKRFSNALYNMGLGK
                     PLGIEIPGEGEPFIRKA"
     gene            complement(1235..1615)
                     /locus_tag="PK976_02930"
     CDS             complement(1235..1615)
                     /locus_tag="PK976_02930"
                     /inference="COORDINATES: protein motif:HMM:NF039650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HRC66872.1"
                     /translation="MKKTNSHIKSKKKHRNSVKSWISGAIVINKEIPIIKVVGFLAFI
                     GLIYIASNYHAMESLRRINQLKTDIKELRYEYINAKSQFTDSTKQSRMIYRASALGLR
                     PSQDPPIRIAVCDEDFKELKKLKQ"
     gene            complement(1612..>2419)
                     /gene="rsmH"
                     /locus_tag="PK976_02935"
     CDS             complement(1612..>2419)
                     /gene="rsmH"
                     /locus_tag="PK976_02935"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HRC66873.1"
                     /translation="GGGHTAEILRQMPSGRVIAFDQDSDVIANLPKDERLVFINDNFR
                     YLKNFLRFYEAIPVDGILADLGVSSYQIDRAAKGFSTRWDGPLDMRMDKRKSLTAAQI
                     INKYSETELFRLFKQYGELAQARRIASIICNERTRMSLETTTQLCELIKPLAPKGAEN
                     KFMAQVFQALRIEVNDELEVLKEFLLQSIDVLCPGGRIAIISYHSLEDRLVKNFFKSG
                     NFEGEVHKDFFGNVVCPLRPVNKKPIEPSPTEIVDNPRARSAKLRIAERL"
BASE COUNT          637 a          535 c          464 g          783 t
ORIGIN      
        1 cggcttttct gatgaagggt tcaccttcgc cggggatttc tataccaagg ggcttcccta
       61 aacccatatt ataaagcgca ttgctgaagc gttttggttt tttcccaaaa gccttgtaca
      121 cgattttcga aactcctaca ttggatgaat gagcgaatgc atcttcaaga gtgatatagg
      181 ttctgttcaa gggatggtca tctttcatta cagcgtttct cgaccagcta atacttcctt
      241 gatacgacac tgaatccttg atattgtatc ctgcatattc cataaccgct accagcgaag
      301 gcaacttgaa gatagaaccg ggttcgatgc tttctgctat ggcataattg tatgtttcgt
      361 cataactgcc atccttgatc tttcgaaggt tcgctatgac tttgatgtcg ccggttgcaa
      421 cttccattag caccacgcaa ccttgatagg catcattttc ggttaattgc tcaagcagag
      481 cttgttcggc aacatcctga agcttgatgt cgatggtagt tacgatatct ttgccatttc
      541 gtggttctat acgttgcgca cccattacag gaatccattc ccctcccgaa atcttttgca
      601 taagcatact gccattgatt ccctttagtt cttcatcgta agctccttcc aatccaacca
      661 aaattccctc ttgtttattt tcgtagccaa tagtacgacg agcaagcatg ccataaggca
      721 tttttctgat gtttttctgt atcaaaatga gaccaccctt gtttttgcct tcacataaaa
      781 taggcagggt cttgattctt tttagagtgg aataatctac attgcgcttg atcaaataat
      841 atcgattgcc ttgtttccgg gcttctttta gctctgattt ccattcattc ctgcttttat
      901 tggggaataa tttcgatagg cccaacgcca gactatcaac tttcttattg aaaaattctt
      961 tcgaaatatt tggattagcc acatccatac gaacatcgaa aataggtaca gtagtcgcca
     1021 gcaggctccc atcagcagca agaatatttc ctctaatggc ttcaatttca tcgaacttga
     1081 aggtagattg tttaaccatt tcacgatatt ccttgccctt tactgcctga atatagatga
     1141 ccttacccaa tattaccata cccaagagaa acattagcca atatatgaac catagacgcc
     1201 agactatttt tttcttgaat gattccatat ctgactattg tttaagtttt ttcaactctt
     1261 taaaatcttc atcacatact gctattctta taggaggatc ttgggagggt cgtagaccga
     1321 gtgccgaagc gcgatagatc attctcgact gcttggtcga atcggtaaat tgcgactttg
     1381 cattgatata ttcgtacctt agttctttga tatccgtctt aagctgattg attcttctaa
     1441 gtgattccat cgcatgatag ttggaagcga tatagatcaa tccaatgaat gctaaaaagc
     1501 caaccacttt aatgatgggg atttccttat tgatgactat agctcccgaa atccagcttt
     1561 ttacactatt cctgtgcttt ttcttcgatt tgatatgtga attggttttc ttcataatcg
     1621 ttcagcaatt ctaagtttag cacttctggc tctcggattg tcgacgattt cggtgggcga
     1681 aggttcgatg ggttttttgt ttacaggcct taacggacaa acaacatttc caaaaaagtc
     1741 tttatgaact tcaccctcga aattcccgct cttaaagaag ttttttacca aacggtcttc
     1801 gagcgaatga taggaaatga tggcgatgcg accacctggg cataatacat cgatagattg
     1861 aagaagaaat tctttgagta cttctaattc atcgtttact tctatgcgca gagcctgaaa
     1921 tacctgggcc atgaatttgt tttcggctcc ttttggggca agaggtttta ttaattcaca
     1981 caactgagtg gtggtttcca gagacattct ggtgcgttcg ttacagatga ttgaagcaat
     2041 gcgacgagct tgcgccaatt cgccatattg cttaaacaac ctgaagagtt cggtttctga
     2101 atatttattg atgatctggg cggctgtgag gctttttctt ttatccatgc gcatatcgag
     2161 gggtccatcc catcgggtcg aaaaaccctt tgccgctcgg tcgatctgat atgaagaaac
     2221 ccctaaatcg gccaaaatcc catctacagg aatagcctcg taaaatctca agaaattctt
     2281 caaataacga aagttatcat ttataaaaac caaacgttca tctttgggta gattagcaat
     2341 aacatcgcta tcctgatcga aagctatgac gcggcctgac ggcatttgcc gcaaaatttc
     2401 agctgtgtgg ccgcctccc
//
LOCUS       DAORPD010000183         2414 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_7892_length_2414_cov_2.870708, whole genome shotgun sequence.
ACCESSION   DAORPD010000183 DAORPD010000000
VERSION     DAORPD010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7892_length_2414_cov_2.870708"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="PK976_02940"
     CDS             <1..431
                     /locus_tag="PK976_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66874.1"
                     /translation="IQQLRFDDKFEYEIEVSPELKTEKIAIPPLLIQPFIENSIEHGF
                     NDIKIKGHLKIVYTRQGKNLMIYILDNGIGLSRSRDQNPDPHKHKPMAIPITKERLEI
                     LNRRFRREQIQFKVTDNLTSGSVYPGTRVEFSIPLIDWKT"
     gene            428..2383
                     /locus_tag="PK976_02945"
     CDS             428..2383
                     /locus_tag="PK976_02945"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRC66875.1"
                     /translation="MKKHTIGLLALIFSFLSSGLSAQNLYIKTDSLQKALPSLKGVER
                     REAMRQIIEYSEEFSKDISKEYSLRLFEEAKMVGDTFYMIEGLYNLGMVEKLDGNFFQ
                     ANQYLNELISLAKKMKNPEFQAKNLTRVSIILSLMDMSDLAFTYLEQAIVLLRNSSDI
                     REIVNLMQTIAQEYYRLRLYHEAIGIYRILKIFLIPQIPANDLSRTIFYRDYAYALCM
                     NDYLTDALYYADKAREIAMKNHSLRDIYNLDRLYGLIYNKMQNNDKVIQYLEPAALQT
                     ENYGYLLKSSYIHNELAHYLQQAGDYSHALEHQLKALRLRQKANFNSVICFAYINYGD
                     ALMKAGKMDSAFIYLSMGKDLASKMNFKTEIRRAYEKLGDYYMRTNRFDKALAFIKEA
                     RDIQDKEAAIQSEQQRKLLALNTEAQSYNEKNLGLKEIESKQNIFFILIAGIILLLIV
                     ILWLYYINHRREDFLRSIEIKQKILASQLNPTFIFNMLVGIRGIIRQGRNEEAGKYLA
                     GFAKLIREILVSSQHNYQFIEREIDMLNSYLELQKIRFSGLLNYEIHLSQSLDSSRMG
                     IPPFLCHSILENFFNFIADKNDKPFHIFIDFQEDNNSIVETIKINLDSEIYHQNLGSL
                     WLDELKPVLELIKERITIICKSKKNHY"
BASE COUNT          818 a          460 c          438 g          698 t
ORIGIN      
        1 aaattcagca acttcgattc gacgataaat tcgaatacga gatagaagtt tcgcctgagc
       61 tcaaaaccga aaaaatagcc attccaccct tacttattca accattcatc gaaaacagta
      121 tagaacacgg atttaatgac atcaaaatta aaggtcatct gaagatagtc tatacacggc
      181 aaggaaagaa tctcatgata tacatactgg acaatggtat tggtttgtca agaagcaggg
      241 atcagaatcc cgaccctcac aaacataaac ccatggccat tcccattacc aaggagcgtc
      301 ttgaaatctt gaaccgccga tttcgccgtg agcaaattca gttcaaggtt accgataatc
      361 ttacctcagg ttcggtctat ccaggcacga gggtagaatt ttcaattcct ctaattgatt
      421 ggaagacatg aagaaacata ctattggcct attggctttg attttttctt ttctctcttc
      481 ggggctttct gctcagaatc tttatattaa gacagatagc ctgcaaaaag cattgcccag
      541 tttgaaagga gtcgaacgca gggaagccat gaggcaaatt attgaatatt cggaagaatt
      601 cagcaaagat atttccaaag aatattcgct tcgattgttc gaagaagcaa aaatggtggg
      661 cgataccttt tacatgattg agggcttgta caatttgggg atggtcgaaa aattggatgg
      721 gaattttttt caggcgaacc agtatttaaa cgaattgatt tccttagcca aaaaaatgaa
      781 aaatcctgag tttcaggcga aaaatcttac gagagtctca ataatccttt ccttaatgga
      841 catgtcagat ctggccttca cttatttgga acaagctatc gttttattga gaaattcgtc
      901 tgatattcgt gaaatagtca atctaatgca aaccattgcc caagaatatt accgcctgag
      961 actttaccat gaagcaatag gaatttatag aatattgaaa atattcctaa tacctcaaat
     1021 tcccgccaat gatttgtcaa gaaccatctt ttacagggac tatgcctatg cactatgtat
     1081 gaacgattat cttaccgatg ccctgtatta tgctgataag gctcgtgaaa ttgccatgaa
     1141 aaatcattcc ctccgggaca tctacaattt agacagactt tatggactca tttataataa
     1201 gatgcaaaat aatgacaagg ttatacaata tcttgaaccg gccgccctgc agaccgaaaa
     1261 ttatggttac cttctgaaga gctcttatat ccataatgaa ctcgcccact acttgcaaca
     1321 agcaggagat tattcccatg cattggaaca tcagttgaaa gcattgcgct tgcggcaaaa
     1381 agctaatttt aactcggtga tttgctttgc ctatataaat tatggcgatg ccttgatgaa
     1441 agctggcaaa atggattcgg ccttcattta tctttcgatg gggaaagatt tggcttcaaa
     1501 aatgaatttt aaaacagaaa taaggagagc ttatgaaaaa ttaggggatt attacatgcg
     1561 tacgaatcga ttcgataaag ctctggcatt tatcaaagaa gccagagata ttcaagataa
     1621 ggaagcagcc attcaatcgg aacaacaacg taaactcttg gctttaaata cagaagctca
     1681 atcctataac gagaaaaatc tcggattaaa agaaatagaa tcgaagcaaa atattttctt
     1741 tattctgatt gcaggaatta ttttactatt gattgttata ttgtggttgt actatatcaa
     1801 tcacagacgc gaagattttt taagaagtat cgaaatcaaa caaaaaatcc ttgcatcgca
     1861 gcttaatcct acgttcattt ttaatatgtt ggtgggaatt cgtggcatta tccggcaagg
     1921 aagaaacgaa gaagcaggga agtatctggc tggttttgcc aaattgattc gagagatact
     1981 ggtaagctcc caacacaact atcaatttat tgagcgagaa atagacatgt tgaatagtta
     2041 tctcgaactc caaaaaatac gtttctctgg gctacttaac tatgaaattc atctcagtca
     2101 atcactcgat tcttctcgta tgggaattcc accgttttta tgccattcca ttctcgaaaa
     2161 cttcttcaac tttatagccg acaaaaacga caaacccttc catattttca ttgattttca
     2221 agaagataat aacagtatcg tggaaactat aaaaataaat ctcgatagcg aaatatatca
     2281 tcaaaatctc ggatcgctat ggctcgatga gttgaaacct gttctcgagc tcatcaagga
     2341 aagaattacc attatttgca aatcgaaaaa gaatcactat taaccgacaa aattctaaaa
     2401 agttcctaaa catt
//
LOCUS       DAORPD010000184         2412 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_7899_length_2412_cov_2.444633, whole genome shotgun sequence.
ACCESSION   DAORPD010000184 DAORPD010000000
VERSION     DAORPD010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7899_length_2412_cov_2.444633"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="PK976_02950"
     CDS             <1..625
                     /locus_tag="PK976_02950"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC66876.1"
                     /translation="STTLKVICGLVLPHFGEILFQGKKINGSQPYQLVRKGLSLVSEG
                     RKVFTSMTVMKNLEMGGYILKKNIANSVNGKNKLKNSIKNIFEIFPVLKERRKQKAGA
                     LSSGEQQLLAIARALILNPKLFLLDEPSLGLSPNNVKIVFEKIREINKKGTTILVVEQ
                     NARMALEYADRGYIFEVGGIIMNDTAKNLLNNEKVKKSFLGEINYFG"
     gene            complement(740..1714)
                     /locus_tag="PK976_02955"
     CDS             complement(740..1714)
                     /locus_tag="PK976_02955"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HRC66877.1"
                     /translation="MKSFKNTLSQLLKALVAVGLIWLIIGKTGFTFKEFLDILKGINL
                     WMYSLALPGVLIVLWLKSRRWHYLMKTEGIHYDTTNTYKSYLTSYSIGLLTPGRFGEI
                     VRVYYIRDLSEGGLFKAFQTVIADRFFDLYFVLGGGLLSYALLLQTENITFLWISVMI
                     VIFGLLILWVLLYCFRNKNQKHNFISFLYGSLYPITGIHSMKNWLLTVFAYMIYFLQS
                     YLIAKALNFHIGFWEMAGVIIITSAVLLIPITWAGFGTRELSLVVLLAHWGISSESAM
                     AFSLLQFVATFFIGGITGLVIWFSHPIPREALKKDYESIKQFLKFRKN"
     gene            complement(1725..>2412)
                     /locus_tag="PK976_02960"
     CDS             complement(1725..>2412)
                     /locus_tag="PK976_02960"
                     /inference="COORDINATES: protein motif:HMM:NF016328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HRC66878.1"
                     /translation="YGNTLHVSSIRSFLAAHKEKDLTSIEKIYFRYGEWPGRMRIEMK
                     NGRIMELPKFHANYLIPFHILKNSLLCTDLTNEFTDISGGDAWAPVYEERGKGFSLVI
                     ARSKQGQNLLEEMANQHIITLWPIAEEEAIKMHSHGYDLKKRGTFIRMQFRSLLGLKN
                     PDYGYKISGFSLGRYMMEILIDLLFLLFGTKPARYMADHIPQKTMGYIFENARKYWKK
                     ATYSTKREKL"
BASE COUNT          799 a          429 c          453 g          731 t
ORIGIN      
        1 gtcgacaact ttaaaggtta tttgtggctt agttttacct cattttggtg agattttatt
       61 tcaaggtaaa aaaataaatg gcagtcaacc ttatcaactt gttagaaaag gattatccct
      121 tgtatctgaa gggaggaaag tgtttacatc aatgactgtc atgaaaaatc tcgagatggg
      181 aggttatatt ttaaagaaaa atattgcaaa ttcagtaaat ggaaagaata aattaaagaa
      241 tagtattaaa aatatattcg aaatttttcc tgtattaaaa gaaagaagaa aacaaaaggc
      301 aggcgcacta agcagtgggg agcaacaact acttgccatt gctagagctt tgattctcaa
      361 tcctaaactt tttttattgg acgagccttc tcttggcctt tcgcccaata atgtaaaaat
      421 tgtattcgag aaaataagag aaattaataa aaaaggaaca actattcttg ttgtggaaca
      481 aaatgctcgg atggctttgg aatacgccga tagaggctat attttcgaag taggcggaat
      541 tatcatgaat gatacagcaa agaatttact aaacaatgaa aaagtaaaaa agtcattttt
      601 aggagaaatt aattattttg gctaattctt aataccttct ctcaataata tttattttga
      661 tttttatttt tataacttta aacaacaaga ttccccctaa tttctatttc attaaagttt
      721 tttcatttta atccagagct taatttttcc ggaattttaa aaactgttta atgctttcat
      781 aatctttttt caaggcttca cgggggatgg ggtgtgaaaa ccatatcact aaacccgtga
      841 ttcctccaat gaaaaaggta gcgacaaatt gaagtaacga aaacgccatg gccgattctg
      901 aacttatacc ccaatgagcc aacaatacta ccaacgataa ttccctggtc ccaaacccag
      961 cccatgtaat tggtattagc aatacagcac tcgtgatgat gatgaccccg gccatttccc
     1021 aaaatccaat gtgaaaattc aaggctttgg ctatcaaata cgattgcaga aaatatatca
     1081 tataggcaaa aacagtaaga agccaatttt tcatggaatg aatgcctgtg ataggataaa
     1141 ggcttccgta caagaatgat ataaaattat gtttttgatt cttgtttctg aaacaataga
     1201 gcaaaaccca taaaatcagt aagccaaaga tgacaatcat cactgaaatc catagaaagg
     1261 taatattttc ggtttggagc aacaaggcgt acgagagcaa acctccaccc agtacgaagt
     1321 acaaatcgaa gaatctgtcg gcaattacgg tctggaaagc cttaaacagc ccgccttcgg
     1381 aaaggtcacg tatgtaataa actcgtacga tttcaccaaa acgtccaggt gtaagcaaac
     1441 caatagaata agaagtgaga taagatttat aagtattcgt ggtatcgtaa tgtatgcctt
     1501 ctgttttcat taaataatgc catctccggc tttttaacca caaaacgata agtacccctg
     1561 gtagagccag agaatacatc cataagttga tgccttttag aatgtcgaga aattctttaa
     1621 aagtaaatcc tgtcttgccg atgatcaacc agattaaacc aacggctaca agggctttca
     1681 acagttgtga gagggtattt ttaaaagatt tcattattcg attttcagag tttttcccgt
     1741 ttggtggaat aagtagcttt tttccaatat tttctggcat tttcgaaaat atagcccata
     1801 gttttctggg gaatatgatc ggccatataa cgtgcgggct ttgttccgaa aagaaggaaa
     1861 agcaaatcga tgagaatctc catcatatat cggcccaggc taaatccgga gattttatat
     1921 ccatagtcgg gattttttaa acccaataga cttctgaact gcatacgaat aaaggttccc
     1981 cgttttttta aatcgtatcc atgagagtgc attttgatgg cttcttcttc ggcgatgggc
     2041 caaagtgtga tgatgtgttg atttgccatc tcctcgagta aattttgtcc ttgtttgctt
     2101 cgagctataa ccaacgaaaa gccttttcct ctttcttcgt aaacaggtgc ccacgcatca
     2161 cctcccgaaa tatcggtaaa ctcattggta agatcagtac ataatagact gtttttaaga
     2221 atatggaaag ggatgagata gttggcatga aactttggta attccattat gcgaccgttt
     2281 ttcatttcta tgcgcatgcg gccgggccac tcaccataac ggaaatagat tttttctata
     2341 gatgtcaaat ctttttcctt gtgtgcagca agaaagctgc ggatggagga aacatgcagt
     2401 gtgttgccgt aa
//
LOCUS       DAORPD010000185         2411 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_7905_length_2411_cov_2.644312, whole genome shotgun sequence.
ACCESSION   DAORPD010000185 DAORPD010000000
VERSION     DAORPD010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7905_length_2411_cov_2.644312"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            110..901
                     /locus_tag="PK976_02965"
     CDS             110..901
                     /locus_tag="PK976_02965"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRC66879.1"
                     /translation="MNIAHIIILISIGLFTGLLTGLTGASGVILVVPCLNILCNFTIH
                     EAIGTSLMVNVITALAISFVYFKHKNIDFGSGLWIAAGSIFGSQIGTMYASGIKGVSL
                     GIAFEIFMIAMGAMIWSKGIRAGSMTKQSKSIFKFRNSILKTLTTLVLGFLVGIMTGI
                     FGAGGGGMILMILIFVLNFPLRLAIGTSSFLMTITALSGVLGYAGHGSVKWASGIIIG
                     LSSAISGIASAKFANRVNDKILSKAMGGIFIFLGVIMFVVQDFKP"
     gene            1468..1854
                     /locus_tag="PK976_02970"
     CDS             1468..1854
                     /locus_tag="PK976_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66880.1"
                     /translation="MITQGSRLYIATQWSKNQAVEEILGLKNFNEDTLYSNLEWLAAH
                     QLEIEKELFKKMRNEIKTVYLYEVTSSYFEGKQNELAAYRFNRYKKKGKMQITIGLLC
                     NEDGEPISVEVFEGNNPEKNSKEPAR"
     gene            1856..>2411
                     /locus_tag="PK976_02975"
     CDS             1856..>2411
                     /locus_tag="PK976_02975"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRC66881.1"
                     /translation="MKNDFDIENVTFVGDKGMLRTEQLNEITNMNWHYITFANKNQMK
                     TLLKRGATQMEIFKDKPMEKSIDGIRFILLRNAVMAKGIQKNRQKKIHDIETKIQQQN
                     IYLYEHPKAKLSTATKNIEKLIERKKLRNCLGINVDEDRRYLELLIDKEALKQEEQLD
                     NCYVIKTDLQATDASKEIVHNRYRN"
BASE COUNT          853 a          338 c          494 g          726 t
ORIGIN      
        1 ggactggatt tttattcgtc tgtgtgtcaa tcgaaagttt tcatgtaaat ttgtaaaatc
       61 ctctatatga ttgtccccct aatatggatt taagccaaaa ttcaaaaaga tgaatattgc
      121 tcatataatt attttgattt cgattggttt gtttaccggc cttttaacgg gtttgacagg
      181 cgcttcaggt gttatcttgg ttgtaccatg cttgaacata ctgtgtaatt ttacaattca
      241 tgaagctatt ggaacgagcc tgatggtaaa cgtaattact gccttggcaa tcagtttcgt
      301 atattttaaa cataaaaaca ttgacttcgg ttcagggcta tggatagcgg caggatctat
      361 ttttggatct cagataggga caatgtatgc ttcaggtata aaaggagtaa gtttaggaat
      421 tgcttttgag atttttatga tagcaatggg ggctatgata tggtcgaaag gaataagagc
      481 tggatccatg acaaaacaat cgaagagtat attcaaattt cgaaattcta ttctaaagac
      541 actaactaca ttggttctag gttttctggt tggcataatg acgggaattt ttggtgctgg
      601 aggcggtgga atgattttaa tgattttgat atttgttttg aattttccac ttcgcttagc
      661 gattggaact tcttcttttt taatgaccat cacagctcta tcaggcgttt tgggttatgc
      721 cggccatgga agcgtaaaat gggcatcagg tattattatt ggactaagtt cggcaataag
      781 tggtatagca agcgcaaaat ttgccaatag ggtgaacgat aaaattctta gtaaagctat
      841 ggggggaatt tttatcttcc tgggtgtcat tatgtttgtc gttcaagatt ttaaaccctg
      901 aaactatcat accaactccc ttaaaaatat tcgaatgatt ttaacttcct gattttcagt
      961 agataatatt ttattttttc gtttaagtga tattcctttc aaatttcttc aaaaaataaa
     1021 atttcgttca cactcgctaa agcaaaattt tgctttttat cgtgtacgca ataaaaaaat
     1081 tttccttaaa aaatttggca aattaacttt ttttgtatta attttataaa ataaatgtac
     1141 acatatatgt atattcaata catcaaaacc aaatataaag ataaggaata tacctctgtg
     1201 atttttgcgg aaagctacca tcaaaatggt aaaatgaaac gcagaatcat tgccaacttg
     1261 agccattgcc caaaagacct ggtggaaaat attcagaaat tgttaaagga tagaaagcta
     1321 aaccatgcga tggaattacc tataaaataa aggaaaagtt ttggtgcgtt atatgttttg
     1381 catataatag ctcagagaat agggttagac aaggcattgg gtaataaaag gaaggcgaga
     1441 ttagccttat tgcaaatata tgcaaggttg atcactcaag gatcgaggtt atacattgcc
     1501 acccaatggt caaagaatca agcagttgaa gaaatactcg ggttaaagaa ttttaatgag
     1561 gatacactgt attcgaattt ggagtggttg gctgctcatc agctcgagat agagaaggag
     1621 ttatttaaga agatgagaaa cgaaatcaaa acggtttact tatatgaggt aacctccagt
     1681 tattttgaag gtaagcagaa tgaactggct gcatacagat tcaatcgata taagaaaaaa
     1741 ggaaagatgc aaattacaat tggattactt tgcaatgaag atggggaacc cataagtgta
     1801 gaagttttcg aggggaataa cccggaaaaa aatagtaaag agccagctcg ataaattgaa
     1861 gaatgatttt gatatagaaa acgtaacctt tgtaggggat aaaggtatgc taaggacgga
     1921 gcaattgaat gaaattacaa atatgaattg gcattatata accttcgcga acaaaaatca
     1981 aatgaaaaca ttactgaaac ggggagcaac acagatggag atcttcaaag ataagccgat
     2041 ggaaaagagt atagacggaa tcaggtttat tttgctgcgc aatgctgtaa tggcgaaagg
     2101 aatccagaag aatcgtcaaa aaaagataca tgacatagaa accaaaatac agcaacaaaa
     2161 tatttatctt tatgaacacc ccaaggcaaa gttatccaca gctactaaaa acatagaaaa
     2221 gttgatagag agaaagaaat taagaaattg cctgggtatc aatgtggacg aagacagacg
     2281 ttacttggaa cttttgatag ataaagaagc attaaagcag gaagaacagt tggataattg
     2341 ctatgtaata aagacagatt tacaagctac ggatgctagt aaagaaatag tacataatcg
     2401 ttataggaat t
//
LOCUS       DAORPD010000186         2401 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_7955_length_2401_cov_2.465047, whole genome shotgun sequence.
ACCESSION   DAORPD010000186 DAORPD010000000
VERSION     DAORPD010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7955_length_2401_cov_2.465047"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..1792)
                     /gene="pruA"
                     /locus_tag="PK976_02980"
     CDS             complement(152..1792)
                     /gene="pruA"
                     /locus_tag="PK976_02980"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018358855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HRC66882.1"
                     /translation="MNNAIFMYDLPKNEPILGYLPCSLERKELEKEIQRLSSEVIEIP
                     LIIGGKEIYTGNLGEVVMPHNHSHVLATYHKAGEKEVKMAIDAALEAHSYWTNVSWVE
                     RMSVTLKIAELISKKYRALLNAATMLGQSKNIYQAEIDCVCETIDFLRFNAYYISEVY
                     NHQPFSESGCINRLEYRPLEGFIFTVSPFNFTSIASNLNMSPVLMGNTVVWKPASTAI
                     LSNYVLMKIYKEAGLPDGVINFLPGQGSFIGNIVLNHRDLAGVHFTGSTATFNEFWKT
                     ISHNIGHYRSYPKLVGETGGKDFVFLHSSADIDEAATALIRGAFEYQGQKCSAASRAY
                     VPRSIWPSLKDKMLGMLHEIKIGDPTNYENFVNAVIDEASFNKIMHYIDYAKGSKEAE
                     IIWGGKGDKSVGYFVDPTIIFTSNPHFLTMEEEIFGPVLTVYIYEDDQFDETLRLCDQ
                     TSPYALTGAVFSKDREALNHACRVLRYAAGNFYFNDKPTGAVVGQQPFGGSRASGTND
                     KAGGPINLIRWTSVRTIKENLNPPIDYRYPYMVNKECK"
     gene            complement(2024..2215)
                     /locus_tag="PK976_02985"
     CDS             complement(2024..2215)
                     /locus_tag="PK976_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HRC66883.1"
                     /translation="MSTKKVFPLRISPEMLDTLQQWANDEFRSINGQIEYILYEALSK
                     ACRLKSSKTQPTDPPEDEF"
BASE COUNT          673 a          506 c          458 g          764 t
ORIGIN      
        1 ataaccattt tgccctttac gagttcttat ttaatctggc agtggaattt ttctcattca
       61 tccattgaat tgcggctgag ccggagcctc agccgcaata aaagaactct tctccagtaa
      121 taaaaatatg atttcgaatc gaaggcattt actacttgca ctccttattt accatatagg
      181 gatatctata atcaatggga ggattgagat tttctttgat agtacgaaca ctggtccagc
      241 gtatcagatt gatgggtcct ccggccttat cgttcgtacc gctggcccta gagccgccaa
      301 aaggctgttg acctactacc gcacccgttg gtttgtcgtt gaaatagaaa ttacctgctg
      361 catatcgaag gacgcggcaa gcatggttca gggcttctct gtctttcgaa aacactgcac
      421 ccgtgagagc ataaggtgaa gtttggtcac aaaggcgaag tgtttcatcg aattgatcgt
      481 cttcgtaaat ataaaccgtg agcacgggtc caaaaatttc ttcttccatg gtcaagaaat
      541 gaggattgga ggtgaagata atggtaggat cgacaaaata accaacggat ttatctccct
      601 ttcctcccca gattatttcg gcttctttag aacctttggc gtagtcgatg taatgcatta
      661 tcttattgaa tgaggcttca tcgattactg cattcacgaa attttcgtaa ttcgtggggt
      721 cgcctatttt gatttcatgg agcattccaa gcatcttatc ttttaaactt ggccatatcg
      781 atcgcggtac ataagcccgt gaagccgccg aacatttttg tccctgatac tcgaaagcac
      841 ctcttatgag ggccgtggca gcttcatcaa tgtcggcact cgaatgaaga aatacgaagt
      901 ctttccctcc ggtttccccc actagcttgg gataggagcg gtaatgacca atattgtggc
      961 taatggtttt ccagaattca ttgaaagtgg cagtggaacc tgtaaaatga accccagcca
     1021 gatcgcggtg attcaagacg atgttgccta taaatgaacc ttgtccgggc agaaagttga
     1081 tgactccatc gggaaggccg gcttctttgt aaatcttcat taacacataa ttcgaaagaa
     1141 ttgcagtgga ggcaggtttc caaactaccg tgttccccat caaaaccggc gacatgttca
     1201 aattggaagc tatggaagta aaattaaatg gcgacactgt aaagataaat ccttctaatg
     1261 gcctgtattc caatcggtta atacaaccgc tttcggaaaa aggctgatga ttgtaaactt
     1321 ctgaaatata ataggcatta aaccgcaaaa aatcaatggt ttcgcaaaca caatcaatct
     1381 cagcctgata aatattctta ctttgtccaa gcatggtagc agcatttaag agggcacgat
     1441 attttttcga aatcaattcc gctattttca gcgtaacact catcctttct acccaagaaa
     1501 cgttcgtcca ataactatgt gcctcaagag cagcatctat ggccatcttt acttcttttt
     1561 ctcctgcctt atgataggtg gccaatacat gagaatggtt gtgtggcatg actacctcgc
     1621 ccaaattccc tgtataaatt tctttcccac ctataataag cggaatttca attacttcac
     1681 ttgataatcg ctggatttcc ttctccagtt cctttcgctc gagtgaacag ggtaaatacc
     1741 caagaattgg ctcattctta ggcaaatcat acataaaaat tgcgttgttc ataaaattta
     1801 ttatttattt tttcgacttg caaagatatt caactcgctt ttaaaaaaca aacatactca
     1861 gaacttttcc ctcattttat tcccaaattg aataaaagaa aattttattc gttatgggtc
     1921 acttcatcac tctgagtaga aaattcagga tggaaagaga ttttgaattt atgattctcc
     1981 tatttatcgt aataacttgg cacaacgtat ttttcctgat tctttaaaac tcatcttcag
     2041 gggggtcagt aggctgagtt ttacttgatt ttaacctgca cgccttcgaa agcgcttcgt
     2101 agagtatata ttctatctga ccgttgatgc ttctgaattc atcattagcc cattgctgaa
     2161 gtgtatcgag catttcgggc gaaattctca atgggaaaac ctttttcgtg ctcatgtttt
     2221 cgagtgaata tttagaacga ttatcttatg tttattcgat gaattttatt tattcggata
     2281 tattttcaaa tccataatgt tgaagtactt gcctgacttt ttcgggctgc ttggttccaa
     2341 tgagaatttg tttcccgttt ttcatcataa tttgtatccc ccagctacca cttaaattat
     2401 a
//
LOCUS       DAORPD010000187         2401 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_7956_length_2401_cov_2.207161, whole genome shotgun sequence.
ACCESSION   DAORPD010000187 DAORPD010000000
VERSION     DAORPD010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7956_length_2401_cov_2.207161"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2157)
                     /gene="alaS"
                     /locus_tag="PK976_02990"
     CDS             complement(<1..2157)
                     /gene="alaS"
                     /locus_tag="PK976_02990"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HRC66884.1"
                     /translation="MKSADIRKTFLDFFAERQHQIVPSAPMVVKNDPTLMFVNAGMNQ
                     FKDIFLGNRPVVFPRVADTQKCLRVSGKHNDLEEVGHDTYHHTMFEMLGNWSFGDYFK
                     EEAIAWAWELLTEVFKIDGSRLYVTVFQGDDADYLPPDQESYKIWQHYQTEEKILFGG
                     KKDNFWEMGDTGPCGPCSEIHIDVRSGEERNAVPGYTLVNKGHPKVIEIWNLVFIEFN
                     RMANGQLQQLPARHVDTGMGLERLCMVLQGKQSNYDTDVFQPIIREIEKLSGKYYGSD
                     PRIDVAMRVIADHLRAVAFAIADGQLPSNNKAGYVIRRILRRAVRYGYNFLGFRQPFM
                     VSLVPILRDQMGSFFPELIAHESLISNVMAEEEANFLRTLESGIQKFNSYISNNQSKG
                     IIDGNFAFELYDTYGFPIDLTQLMAAEQGWEVDMPSFYQHLEEQKQRSRADAAINTDD
                     WIEVVSGLGETTFIGYDTLEAEAEIIKYRYVSTAKKQYFQIVFNHTPFYAESGGQVGD
                     RGWIIAGDEKIEIENTIKENNLVIHIVSRLPLDVSQPVLLKVDEVKRRLTANNHSATH
                     LLHAALRKVLGTHIEQKGSLVDENRLRFDFSHFTRLTSDEILKIEAMANGKIWENLPL
                     KEWRNLPMEEAQKMGAMALFGERYGEMVRVVAFDPEYSVELCGGTHVKATGQIGLIKI
                     ISETGIAAGIRRIEAVTGTVAVAFYNQQLQILEEIKT"
BASE COUNT          701 a          549 c          430 g          721 t
ORIGIN      
        1 ggtttttatt tcttcgagaa tttgcagctg ttgattataa aatgccacgg ctactgtgcc
       61 cgtaacagct tcgattcggc ggataccagc agctataccg gtttcagaaa taatttttat
      121 caatccaatc tgtcctgttg ctttcacatg agttccccca cacaattcga ccgaatactc
      181 tggatcgaaa gccaccaccc taaccatttc gccgtatctt tctccaaaaa gtgccatggc
      241 tcccattttc tgagcttctt ccattggaag gtttctccat tccttcaatg gcaggttttc
      301 ccaaattttt ccgttggcca tggcttcaat cttcagaatt tcatcggagg ttaagcgtgt
      361 aaaatgagaa aagtcgaagc gcaagcgatt ttcgtccacc aatgatccct tttgttctat
      421 atgagttcct aaaacctttc gtaaggctgc atgaagcaaa tgagtagcac tgtgattatt
      481 agccgtaagc cggcgtttta cttcatctac ctttaaaagc actggttgac tcacatccaa
      541 cggaagacgt gaaacgatat gaattacaag attgttctct ttgattgtgt tttctatttc
      601 aattttttca tctccagcga ttatccatcc acgatcgcca acctgtccgc ccgattctgc
      661 ataaaaagga gtatggttga aaacaatctg gaaatattgt ttcttggccg tggacacata
      721 ccgatatttg atgatttcgg cttcggcctc cagtgtatcg tatcctatga aagtagtttc
      781 accaaggccc gaaactacct ctatccaatc atcggtattt attgcagcat cagcacgcga
      841 acgttgtttc tgctcttcga ggtgctgata aaacgaaggc atatctactt cccaaccttg
      901 ttcagcagcc atcaattggg ttagatcgat cgggaaacca taagtatcgt aaagttcaaa
      961 ggcaaagttg ccatcgatga tgcctttcga ctgattattt gagatatagg aattgaattt
     1021 ttgaattccc gattcgagag ttcgtaaaaa attggcttct tcttcggcca tcacattgct
     1081 aattagtgat tcatgggcaa ttaattcggg gaaaaacgat cccatttgat ctctcagaat
     1141 gggaaccaaa gaaaccataa atggttgacg aaatcccaaa aaattatagc cataacgaac
     1201 tgcgcgtcgt aaaattctcc taatcacata tcctgccttg ttgttggatg gaagctgacc
     1261 atcggctatt gcaaaggcca ccgccctcaa gtgatcggct atgactcgca tagcaacatc
     1321 aattctggga tcgctaccat aatacttgcc cgataatttt tcaatttctc gaataatagg
     1381 ttgaaaaaca tcggtatcat agttgctttg cttgccttgc aataccatgc ataatcgttc
     1441 gagacccatt cctgtatcga catggcgggc tggaagttgc tgcagttgac cattagccat
     1501 acgattgaat tcgatgaaaa ccagattcca aatctcgatt accttaggat gccccttatt
     1561 gaccaaagta tatccgggta ctgcattcct ttcttctccc gaacgaacgt caatatgaat
     1621 ttccgaacat ggtccacaag ggccggtgtc acccatttcc caaaaattat cttttttccc
     1681 tccgaacaga atcttttctt cagtctgata atgttgccag atcttgtatg attcctgatc
     1741 gggaggtaaa tagtcggcat catctccctg aaaaaccgta acataaagcc ggctcccatc
     1801 aattttgaaa acttcagtaa gcagctccca tgcccatgca atggcctctt ccttaaagta
     1861 atcaccaaac gaccagtttc ccaacatctc aaacatggta tggtgatagg tatcatgacc
     1921 cacctcctcc agatcattgt gcttaccaga aactctaagg catttttgtg tatctgccac
     1981 acgggggaaa acaactggcc gattgcccaa aaaaatatcc ttgaattgat tcatgcctgc
     2041 attgacaaac attaaggtag ggtcattctt gactaccatt ggtgccgaag gcactatttg
     2101 atgctgtcgc tcagcaaaaa aatccagaaa agttttacgt atatccgctg atttcattat
     2161 ttaattcgtt cctttcaatt aaaccatcat cttttgaaaa actcataaat aaatatatgt
     2221 gccaaaacag gaacatccat aagaatcaaa ccttcaaaat actttatata taaaaatttt
     2281 ccctttaaag ggcgcaaatt tacgtgaaaa tattattttt gcgatcgaag atgattttaa
     2341 atcattcaat aattaaaaaa atctgaagca ttatgagacg aaaaatacga tattactttg
     2401 a
//
LOCUS       DAORPD010000188         2395 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_7985_length_2395_cov_2.540598, whole genome shotgun sequence.
ACCESSION   DAORPD010000188 DAORPD010000000
VERSION     DAORPD010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7985_length_2395_cov_2.540598"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="PK976_02995"
     CDS             complement(<1..429)
                     /locus_tag="PK976_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HRC66885.1"
                     /translation="MTWLQALIIAIVEGITEFLPISSTGHMIITEAVLGIEPSEFSKT
                     FIVNIQFGAILSVIVLYWKNFFASLDFYYKLFVAFLPAAVVGYLAGDLIDRMLGSVLV
                     VAISLVVGGIVLLFIEEWFKNTAEKGKNDVTYSSALKIGFF"
     gene            complement(426..713)
                     /locus_tag="PK976_03000"
     CDS             complement(426..713)
                     /locus_tag="PK976_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HRC66886.1"
                     /translation="MEKKTIVTSKKAKEEEKPQAGFAFERQNYILMVIGIVVLIIGFL
                     LMIGGGSDDPQVFNYDLFNFRRLTLSPILIIAGFIIEIFAIMWNPGKKVKK"
     gene            complement(727..1602)
                     /locus_tag="PK976_03005"
     CDS             complement(727..1602)
                     /locus_tag="PK976_03005"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HRC66887.1"
                     /translation="MSQEETLTRKRLRSAYITTIVSLTLVLFMLGLWGVIGLHAHKIS
                     RYVKENLGLTIYFQKNARETEILRLKKSLDTTRWVTSTHYISPEEAEQILKKELGEDF
                     IGFLGYNPLLPCLQIRLNADYTHPDSIQYIEKHLKQNPKVAQIEYQRDLINSVNKNLN
                     IISIIIAVLSFLMFLISIGLINNTIKLSVYSKRFIIRSMMLVGATRGFIRAPFVKKGL
                     WHGILSAIFAIILLSILLYAAYLKIPELKTLADFHILMIIAGAMIVTSALISVISNYI
                     AVTRFLNMNIDKLYM"
     gene            complement(1658..>2395)
                     /locus_tag="PK976_03010"
     CDS             complement(1658..>2395)
                     /locus_tag="PK976_03010"
                     /inference="COORDINATES: protein motif:HMM:NF012562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66888.1"
                     /translation="ETHFENNLPILLALLSIWYTNFFDCATEAILPYDQYLERFPAYL
                     QQTIMESNGKSIDRSGLPVSYKTSPVIWGEPGTNGQHSFYQLIHQGTQMIPCIFMAAA
                     KPLTPLSEHHQLLLSNFFAQPEALMKGKTAEEVRAEMLSKGFNEEEINKLLPFRVFRG
                     NIPSISLLYQQLTPYNLGAITALFENRTFVQGIIWNIFSFDQWGVELGKSLANNIIPE
                     LAHDDPIQTHDGSTRQLIHKVKQMRKS"
BASE COUNT          755 a          454 c          483 g          703 t
ORIGIN      
        1 gaagaatccg attttcaggg ccgaagaata ggtaacatca ttttttccct tctcggcagt
       61 atttttgaac cattcttcaa tgaataaaag cacgattcct ccaacaacca atgagatagc
      121 cactaccaat acacttccca gcatgcggtc gatgaggtcg ccagccagat atccaactac
      181 cgcagcaggt aaaaaagcca caaataactt gtagtaaaaa tcaagtgaag cgaaaaaatt
      241 cttccaatac agaacaatta ccgacagtat ggctccaaat tgtatgttga cgataaaagt
      301 cttcgaaaac tcactgggtt caatgcccaa gacggcctcg gtgataatca tgtggccagt
      361 ggaactgatg ggtaaaaatt cggtaatgcc ttctacaata gcgataatta gagcttgtag
      421 ccaggtcatt ttttaacttt tttcccaggg ttccacatga tggcaaagat ttcgatgatg
      481 aatccagcaa taatcaagat gggtgaaagt gtaagtcgcc tgaaattgaa taaatcgtaa
      541 ttaaatactt gagggtcatc acttcctccg cctatcatca ataaaaagcc aatgatgaga
      601 acgactatgc caataaccat cagaatatag ttctgacgtt caaaagcaaa tccggcttga
      661 ggtttttctt cttcttttgc ttttttagag gtaactatcg tttttttttc cataagagaa
      721 aagttattac atatacagct tgtcaatatt catattgagg aaacgcgtaa cggcgatata
      781 attcgaaatt accgagatca atgcgctggt aactatcata gctccagcaa ttatcattag
      841 aatatgaaag tcggccaatg ttttaagttc gggaattttc aaataagcag catagagcaa
      901 aattgaaagc aagatgatag caaaaatcgc actgagtata ccatgccaca aacccttttt
      961 aacaaatgga gctcttatga aaccgcgagt ggctcccacc agcatcatac ttctaatgat
     1021 aaagcgttta gaatagactg aaagttttat ggtattattg atcaaaccaa ttgaaatgag
     1081 aaacatcaaa aacgatagaa cggcaataat gatactgatg atattcagat ttttatttac
     1141 actgttgatg aggtctcttt gatattcgat ttgggccact ttaggatttt gcttaaggtg
     1201 cttttcgata tattggatgg aatcgggatg ggtataatcc gcatttaatc ttatttgcag
     1261 gcaaggcaag agggggttat agccgaggaa accaataaaa tcctctccaa gttccttttt
     1321 tagtatttgc tcggcttctt caggcgatat ataatgtgtc gaggtaaccc acctcgtggt
     1381 atccaagctt ttttttaaac gcagaatttc agtttcacgt gcattcttct ggaaataaat
     1441 ggttaaacca agattctctt ttacatacct cgatattttg tgtgcatgaa gcccaatcac
     1501 tccccaaagc ccaagcatga aaagtaccag tgttagactc acgatggtag taatatatgc
     1561 tgagcgtaag cgtttgcgtg taagtgtttc ttcctgcgac aaagaaataa tgtttaaaga
     1621 ttctgcaaat ttaggaatta aagcctttag aaaaggatta ggattttctc atttgtttca
     1681 ccttgtgaat caattggcga gtagaaccat catgtgtttg aatgggatca tcgtgggcca
     1741 gttcgggaat gatattgttg gcaaggcttt tcccgagttc aacaccccac tggtcgaaac
     1801 tgaagatatt ccaaatgatg ccctgtacga aagttctatt ctcgaaaaga gctgtaatgg
     1861 ctccgagatt ataaggcgta agttgctgat aaagcaagct tatcgaagga atatttcctc
     1921 taaaaacgcg gaatggaagt aatttattaa tttcttcttc attaaaacct ttcgacaaca
     1981 tttcagctct cacttcttcg gctgtcttgc ccttcatcaa agcttcgggc tgagcaaaga
     2041 aatttgataa caaaagctga tgatgttcac ttaatggagt taaaggtttg gcggcagcca
     2101 taaaaataca aggaatcatt tgagttccct gatgaatgag ttgatagaac gaatgttgac
     2161 catttgtacc aggctcaccc caaattaccg gacttgtttt gtaggaaaca ggcaatccgc
     2221 tgcggtcgat cgacttgcca ttactttcca taatggtttg ttgcaaataa gcaggaaatc
     2281 tttcgagata ctggtcgtaa ggcaaaatgg cttctgtggc acaatcgaag aagttggtgt
     2341 accaaatgct caaaagagcc agcaatattg ggagattgtt ttcgaagtga gtttc
//
LOCUS       DAORPD010000189         2395 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_7987_length_2395_cov_2.357265, whole genome shotgun sequence.
ACCESSION   DAORPD010000189 DAORPD010000000
VERSION     DAORPD010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7987_length_2395_cov_2.357265"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..1217)
                     /gene="dprA"
                     /locus_tag="PK976_03015"
     CDS             complement(123..1217)
                     /gene="dprA"
                     /locus_tag="PK976_03015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HRC66889.1"
                     /translation="MSLLYEIALTLIPGVGDINGKKLVNYCGSAEAVFKEKKSALMRI
                     PGIGEYTANAILNTKPFDRAQQEIEFIKKYKVNVFYYKDKNYPLRLRNCEDAPILIYY
                     KGTESLNPPLSLSIVGTRRATDYGKWYIDKLMEALSSYSVVIISGLAFGIDTAAHRSA
                     LEHGLPTIAILGHGLDRIYPSQNRQLAKKILDHGGLLTDFPSQTKPDRENFPKRNRII
                     AGLCDALLVIETGIKGGSLITADIANSYNRDVFALPGRFGDQMSEGCNWLIRTNRASL
                     LDSPQVLLDDLGWAQVEGGTRSAQSKLFINYTEDEKKIVEILNRDSTVSIDKICIESQ
                     LPSTKVVAALLNLEFQGIVKCLPGKVYQLI"
     gene            1558..>2395
                     /locus_tag="PK976_03020"
     CDS             1558..>2395
                     /locus_tag="PK976_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66890.1"
                     /translation="MLIAPLNWGLGHATRCVPLIEAFLKRNVEIILAGEGLVFDFLRH
                     RYPFLHIINLPGQRITYQVRGSLILALAKSFPGFLRNIELEHHIIKDLIEKYRLGAII
                     SDNRYGLWDENIPSFMITHQINVRGKGLWRIGEPFAHAGIRRFLNHFHEIWIPDFPQF
                     PGLAGDLSHDKSPRLKYRYLGPLSRFEHAAPIYEHPQNDIMAIISGPEPQRTIFENIL
                     TRTFEGTNLKTIILRGLPSHQESIVKINRITLYSHLQDDDFIEVLKNSRHLVCRSGYS
                     SLM"
BASE COUNT          695 a          504 c          460 g          736 t
ORIGIN      
        1 cctccttctg acgacatcct gcctttggta aaaaagaaac tgggtatcaa agaataagaa
       61 gttttctatt gcttaattta aaaactgctt ccgaatgaaa tgaagcggtt tttttaggaa
      121 acttagataa gttgataaac ttttcccggc aaacatttta cgataccctg aaattcgaga
      181 ttgagcaaag cagcaactac cttggtggat ggcaattgag attcgatgca gattttatct
      241 atactaaccg tggaatctcg attcaggatt tcgacgatct tcttttcatc ttctgtataa
      301 tttataaaaa gtttcgattg tgccgatcga gtgccaccct ccacttgtgc ccatcccaaa
      361 tcatccagca atacttgggg agaatcgagt aatgaagcac gattggtgcg tatcaaccaa
      421 ttgcagcctt cactcatttg atctccaaaa cggcccggca gggcaaaaac atcacgatta
      481 tatgaatttg caatatcggc cgtgattaat gaacctccct ttattcccgt ttctattacc
      541 agcaatgcat cgcataaacc ggcaataata cgattgcgtt tcgggaaatt ctcacgatca
      601 ggcttggttt gagatggaaa atccgttaat agcccaccgt ggtcgagaat ttttttagct
      661 aattgtcgat tttggctggg atagattcta tcaaggccat gacctaaaat ggcaatcgtc
      721 ggcaaaccgt gttccagagc cgatctatgg gcggccgtat ctatcccgaa cgccaatccg
      781 cttataatca ccaccgaata agatgaaaga gcttccatca gcttgtcgat gtaccatttc
      841 ccatagtccg ttgcacgtcg agtgcctact atactcaaag acaaaggcgg attaagggat
      901 tcggtgcctt tgtagtaaat caaaatcgga gcatcttcac aattcctcag tctcaaggga
      961 taatttttat ccttgtaata gaaaacatta accttgtatt ttttgatgaa ttcgatttct
     1021 tgttgtgccc tgtcgaaagg ttttgtattc aaaatggcat ttgccgtgta ttcaccaatt
     1081 cccggaatcc gcattaaagc agatttcttt tccttgaaaa ccgcttcggc actcccgcaa
     1141 taattcacca gttttttccc attgatatca cctacacctg gaataagcgt caaggctatt
     1201 tcataaagta acgacataag ctgttgtttg gtaagcgaaa ttaaacatta taaccaaaat
     1261 cggcctatac taataaaaac tcgaaaccat cctccactct tatattttca cctatatgtt
     1321 atcggaatca tcggcttatg tcaaatttta tttctaaatt aatgtttcca atttcattat
     1381 tttgattcgt tcaaagcaaa ttcttccgac ttgaatgcct acctttgcca cattctcgaa
     1441 tgaaagatct tggaaaaatt cagaattcgg aatgtttttc gtaatccatc aatatcgacg
     1501 tcgggaattc cggaaaggaa ttgcaaaacg ttcgagcggg aatgaagtat gaaaagggtg
     1561 ttgatagcgc cattgaattg gggccttggt catgccacac gttgtgttcc tctgatagag
     1621 gctttcctga aacgtaatgt cgaaattata cttgcgggcg aaggtctggt tttcgatttt
     1681 cttcgacatc gttatccttt tttacacatt atcaaccttc cgggacaaag aattacctat
     1741 caagttcgcg gcagtttgat ccttgccttg gcaaaatctt ttccagggtt cctacggaat
     1801 atagagctcg aacatcatat catcaaagat ttgattgaaa aatatcgatt gggagctatt
     1861 atttcggata accgatatgg tttatgggac gaaaatattc catcatttat gatcactcat
     1921 caaataaacg ttagagggaa gggattgtgg aggattggcg aaccatttgc ccatgccggg
     1981 attcgtagat ttttgaatca ttttcatgaa atatggatac ccgattttcc tcaattcccc
     2041 ggattggccg gagatttatc gcacgataag agccctcggc taaaataccg ctatttaggt
     2101 cctttatcaa gattcgaaca tgcggcaccg atttacgagc atcctcaaaa tgatattatg
     2161 gccattattt ctgggccaga acctcagcga acgatctttg aaaatattct tactagaacc
     2221 ttcgaaggta ctaatctgaa aacgattatt ctcagaggat tgccttcaca tcaagaatct
     2281 attgtaaaaa tcaatagaat taccctttat tcgcatttgc aagacgatga ttttatcgaa
     2341 gtgctgaaaa atagccgaca tcttgtgtgc cgttcggggt attccagtct tatgg
//
LOCUS       DAORPD010000190         2383 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_8046_length_2383_cov_1.912371, whole genome shotgun sequence.
ACCESSION   DAORPD010000190 DAORPD010000000
VERSION     DAORPD010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8046_length_2383_cov_1.912371"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="PK976_03025"
     CDS             <1..189
                     /locus_tag="PK976_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRC66891.1"
                     /translation="YLIGKISITDLNIAQSEKDLARMNFLRALQRYWNSYYTIRKLTH
                     FDFTNNLPIRVDLNKIIE"
     gene            281..916
                     /locus_tag="PK976_03030"
     CDS             281..916
                     /locus_tag="PK976_03030"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005777720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HRC66892.1"
                     /translation="MSVLVGKKAPLFYADAVINGSQFVEKFSLEQYIGKKYVVFFFYP
                     MDFTFVCPTEILAFQEKLKEFEDRNCAVIGCSVDSKFSHWAWLNTPKEKGGIQGVTFP
                     LVSDFSKTISENYDVLAGYYDYDVEDEVSTFIGEPVAYRGLFLIDKQGIVRHQIVNDT
                     PLGRNVDEAIRMVDALQFYEEHGEVCPANWHKGEEGIKATKEGIAEYLSKH"
     gene            1103..1456
                     /gene="rplS"
                     /locus_tag="PK976_03035"
     CDS             1103..1456
                     /gene="rplS"
                     /locus_tag="PK976_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004330114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HRC66893.1"
                     /translation="MNKPELVKYVESLEEFKQFPEFKAGDTITVDYTIKEGNKTRTQR
                     FTGIVLQIKGTGNTKTFTVRKISGNIGVERIFPFASPNIERIEVNKRGAVRRARIFYL
                     RKLRGKKAKIKEKRS"
     gene            1825..>2383
                     /locus_tag="PK976_03040"
     CDS             1825..>2383
                     /locus_tag="PK976_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66894.1"
                     /translation="MKKIWHCLYVISFVSGFQITLLAQSNISVALIIPDNYASIETRC
                     AKQFLQNLPDVNGFYLNPEDMVNNPNILDQIDVFWIHSNDSACLSQIAKNKVIWSKIN
                     KAVENGKGLLLSNNAVQLLPILGLESHKPQTRTKTLKDKGYGRMAGFHAFRSHPIFKD
                     LNQGVYCLKPTRDITLEVIGYFGDDS"
BASE COUNT          741 a          472 c          436 g          734 t
ORIGIN      
        1 tatctcatcg ggaaaattag catcaccgac ctcaacatag ctcaaagtga aaaagacctg
       61 gctcggatga actttctaag agccttgcaa cgttattgga atagttatta caccattcgc
      121 aagctcactc acttcgattt taccaataat ctacccattc gggtggactt gaacaaaatt
      181 atagaataga ttggaatcag caactaaagt gtgatatact tggttaacac cattaacaat
      241 aaatcaataa acatttcaat aacttaaaat taaatcgatt atgtcagttt tagtaggaaa
      301 aaaagcccca ttattttatg cagatgcagt cataaatggg agtcagttcg tcgaaaaatt
      361 ttcgctcgaa cagtacattg gtaaaaagta tgtcgtcttt ttcttctatc caatggattt
      421 tacctttgtt tgtccaaccg aaattcttgc atttcaagaa aaactgaagg aattcgaaga
      481 tcgtaactgc gctgtaatag gatgctcggt cgattcgaaa ttttcacatt gggcatggtt
      541 gaacacaccg aaagaaaaag gcggaataca gggtgtaacc tttcctttgg tttccgattt
      601 ctcgaagact atttctgaaa attatgatgt tttggctggt tattatgatt atgatgtcga
      661 agacgaagtt tcgacattta ttggagaacc cgttgcttat cgcggtctct tcctcatcga
      721 taaacagggc atcgtaaggc atcagattgt caatgatacg cctttggggc gtaatgttga
      781 cgaagcaatt cgcatggtcg atgctctcca attttacgaa gaacatggag aggtttgtcc
      841 cgccaactgg cacaaaggag aggaaggaat aaaagcaact aaagaaggaa tagcagaata
      901 tttgagtaaa cactagcctt tgcttcattt cctaaaaagc aggtgataca cattcatgga
      961 gactccatat ctttatggac agttgatgca acctgatttt ttttcagaaa gttttcttga
     1021 aaactttatc gttgccttgt gagttgaaaa attgtttgta tttttgcact ccttttttaa
     1081 tgaaagaaat ttttttaaca tcatgaataa gcctgagtta gtaaaatacg tcgaatcgct
     1141 cgaggagttc aaacaattcc ctgaattcaa ggcgggagat accatcactg ttgattatac
     1201 catcaaagaa ggaaacaaaa cccgtacgca gaggtttacg ggcattgttt tgcaaataaa
     1261 aggcacaggc aatacaaaaa cttttaccgt acggaaaatt tcaggtaaca taggtgtgga
     1321 gcgcattttc cccttcgctt ctcccaatat cgaacgtata gaagtcaaca aaagaggagc
     1381 tgttcgacgc gccagaattt tctatttgag aaaacttcgt ggcaaaaaag caaaaatcaa
     1441 agaaaagcgt tcttgatgct tgcttccaaa tttgcaccta atgcataata tacgggcctt
     1501 cccaaaggtc cttttttatt ttatcctata aattcgaaga cggatcgttt tttatttgcc
     1561 atcagccttc agcttattag atataatggc aaggagcatt ccctttcgct gatttacaca
     1621 cttttcaaat tacaaaatat aaattacaaa ttacaatatc tcctttcctc ctttatatac
     1681 ttttaacttt cttctctcca accagcatcc tccagtctcc tccagcatcc tccagtctcc
     1741 tcgtctcctc gtcccccctc ttctatttta ctcaaaatca tttcaagaat tatcactaat
     1801 ttcattgccc gatattttaa tgaaatgaaa aaaatttggc attgtctgta cgttatttcg
     1861 tttgtttcgg gatttcagat tactcttctg gctcagtcga atattagcgt cgccttgata
     1921 attcctgata attacgcttc gatcgagacg cgatgtgcga agcaattcct tcaaaacttg
     1981 cccgatgtaa atggatttta tttaaaccct gaagacatgg tcaataatcc aaatatactc
     2041 gatcaaatcg atgttttctg gattcattcg aatgactcgg cgtgtttatc acaaatagcc
     2101 aaaaataagg ttatatggag caaaataaac aaggccgtcg aaaatggcaa gggtttgctc
     2161 ttgagtaata atgctgttca gcttctaccc attttaggat tagagagtca taaacctcaa
     2221 acaagaacaa agacattgaa agacaagggt tatggccgca tggcaggttt tcatgccttt
     2281 cgttcgcatc ctatcttcaa ggacttgaat caaggagttt actgtttaaa acccaccagg
     2341 gacattacgc tcgaggtcat tggatatttc ggtgatgatt cat
//
LOCUS       DAORPD010000191         2381 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_8058_length_2381_cov_2.401978, whole genome shotgun sequence.
ACCESSION   DAORPD010000191 DAORPD010000000
VERSION     DAORPD010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8058_length_2381_cov_2.401978"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="PK976_03045"
     CDS             complement(<1..704)
                     /locus_tag="PK976_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005777517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HRC66895.1"
                     /translation="MKSGIGKISQIIGPVIDVTFKEADELPNIYESLIVKRPDGTDLV
                     LECQQQIGEATIRAIAMDSTDGLMRGLNVIKTGKPISMPVGENIRGRLFNVIGQTIDG
                     LPNIEVKQTYAIHRDPPKFENLTTNNEVLFTGIKVIDLIEPYAKGGKIGLFGGAGVGK
                     TVIIMELINNIAKRYSGLSVFGGVGERTREGNDLLREMISSGVIRYGHEFIDEMEKGG
                     WDLSKVDMEELAKSQAT"
     gene            complement(753..1409)
                     /locus_tag="PK976_03050"
     CDS             complement(753..1409)
                     /locus_tag="PK976_03050"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HRC66896.1"
                     /translation="MKSKNKAIWLAIGTVALVLFVDQVLKIWIKTHFFLGEEIPVFDN
                     WFFLHFVENEGMAFGFSFGGNSGKILLSLLRIVAVLAIGYYLYLLIKRESKPLKIIVF
                     SLIFAGALGNIIDSCFYGLIFTESTYYQIASFTLKNGYAPFLHGRVVDMLYFPLIETQ
                     WPTWFPFIGGQEFQFFRPVFNIADSAITIGVFMLIIFYKNIFEVSAQKEAIHKIKEEY
                     "
     gene            complement(1438..1836)
                     /locus_tag="PK976_03055"
     CDS             complement(1438..1836)
                     /locus_tag="PK976_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HRC66897.1"
                     /translation="MKKEEAPKERYSDEELEEFRQIIMVKLEKARKDLALLMEAYANL
                     EENGTSDTSPTFKVLEEGNAVLTKEENGKLAARTEKFIKALENALVRIENKTYGICRE
                     TGTLIPKERLRAVPHATLSIDAKLKQKRNK"
     gene            complement(1871..>2381)
                     /locus_tag="PK976_03060"
     CDS             complement(1871..>2381)
                     /locus_tag="PK976_03060"
                     /inference="COORDINATES: protein motif:HMM:NF039585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HRC66898.1"
                     /translation="KKLGPKLGKKMKSVANALENLSQEQIRELEQKQSIQLKIDGDLV
                     LIHLEEVEIITEDIPGWISITETPYTVALDLTLTDDLIDEGYVRDLINRVQNIRKESG
                     FDVTDKIQLKIYHHPTLTKAIEKNYHYICSETLAKELLFTDQLEQEMIEVDIANEFKV
                     FIKVNRIEN"
BASE COUNT          683 a          511 c          384 g          803 t
ORIGIN      
        1 gtagcttgcg atttagcaag ttcctccata tctaccttgc ttaaatccca tccgcctttc
       61 tccatctcat cgataaattc atgaccataa cggattacac ctgatgaaat catttcacgc
      121 aataagtcat tcccttcacg tgttctttca ccaacccctc caaaaaccga taaacctgaa
      181 tagcgtttgg caatattatt tatcagctcc atgataatta ctgttttccc tacgccagct
      241 cctccaaata atccaatttt acctcctttg gcataaggtt caattaaatc gattactttg
      301 atgcctgtga acagaacttc attattggtt gtgagatttt caaatttagg aggatcgcga
      361 tgaatcgcat aagtttgttt tacttcgata ttgggaagcc catcaatggt ttgcccgatt
      421 acgttgaaca atcttcctct aatgttttcg cccacgggca tactgatagg tttaccggtt
      481 ttgatcacgt tgagaccacg catcaatcca tccgtcgaat ccatggcaat ggctcttata
      541 gtggcttcac ctatttgttg ctgacattca agaaccagat cagtaccatc gggacgcttt
      601 acaatcaaag actcataaat gttgggaagc tcatcggctt ccttgaatgt tacatcaatt
      661 accggcccaa tgatttgaga tatttttcct attccacttt tcatcatgaa taaatttact
      721 tggcaaaatt aacattgtta aagcatcaat atttaatatt cttcttttat tttatggatt
      781 gcctctttct gagctgaaac ttcaaaaata ttcttgtaaa aaatgatcaa cataaacacc
      841 cctatggtga ttgcagaatc ggcaatatta aacacaggcc taaaaaactg aaattcctgg
      901 cccccaataa acgggaacca agttggccat tgggtttcaa tcaatggaaa ataaagcata
      961 tccacaactc taccgtgtaa aaaaggggcg tacccatttt tcaatgtgaa tgaggcaatt
     1021 tgataataag tactttcagt gaatataaga ccatagaaac aactatcgat gatattgccc
     1081 agtgctccag caaaaatgag actaaaaact attatcttta aaggttttga ttctctttta
     1141 ataagtagat ataagtaata cccaatagct aaaacagcta caattcgcaa gaggcttagt
     1201 aatatctttc ccgaattgcc tccaaagcta aaaccgaaag ccattccttc attttcgaca
     1261 aaatgaagga aaaaccaatt atcgaaaact ggaatttctt ctcctaaaaa gaagtgtgtt
     1321 ttaatccaaa ttttcaagac ttgatcaaca aacaagacga gggcaacagt tcctatagca
     1381 agccagatgg ctttattttt cgacttcatt ctatggagta cgaagagtaa tcaaaattca
     1441 tttattccgt ttctgtttta gcttagcatc aatcgaaagc gtagcatgag gaactgctct
     1501 caatctttct tttggaatga gagtgcctgt ttctctgcat attccgtagg tcttgttttc
     1561 tattcgtacc aaggcattct cgagtgcttt gatgaacttt tcagttcttg cagcaagttt
     1621 gccattttct tctttggtta gcacggcatt gccttcctct aatactttga aagttgggct
     1681 tgtatcgctg gtgccatttt cttccagatt tgcataggct tccataagta aagcaagatc
     1741 tttacgcgct ttttccaatt ttaccataat gatttggcga aactcctcca attcttcatc
     1801 ggaataacgc tcttttggag cttcttcttt tttcattgta ttccctccta ttagttaaaa
     1861 tttcatcatg tcaattttct atccgattca ctttaataaa taccttgaat tcattggcta
     1921 tatctacttc tatcatttcc tgttccaatt gatcggtaaa taaaagttct ttggccaatg
     1981 tttcggagca aatgtagtga taattttttt caattgcctt agtcagggtt ggatgatggt
     2041 atatcttcaa ttgtatcttg tctgttacat caaatccact ctcctttctt atattttgca
     2101 cccggttgat taaatctctt acataccctt cgtcgataag atcatccgtc aaggtaaggt
     2161 cgagagcaac ggtatagggt gtttcggtta tactaatcca gccaggaata tcttcggtta
     2221 tgatttcaac ttcttccaaa tgaatgagaa ctagatcccc atcaatcttc agctgaatag
     2281 actgtttctg ctcaagttct ctgatttgtt cctgagaaag attctccagg gcattagcca
     2341 cacttttcat tttcttgccc aacttaggcc ctaacttctt g
//
LOCUS       DAORPD010000192         2357 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_8173_length_2357_cov_2.705474, whole genome shotgun sequence.
ACCESSION   DAORPD010000192 DAORPD010000000
VERSION     DAORPD010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8173_length_2357_cov_2.705474"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="PK976_03065"
     CDS             <1..605
                     /locus_tag="PK976_03065"
                     /inference="COORDINATES: protein motif:HMM:NF040342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6175 family protein"
                     /protein_id="HRC66899.1"
                     /translation="ALKRVAKADIILDLDFDIKRQGPQKYIVFNLKGLDAYTAKQVTG
                     VAGAGNPSSAASPELLLEEAVLSHMDNFNAGLMRHFDDMFANGREVKVMVKVWANWGQ
                     NLESEFGPDSKELSEIIEDWFFDNCVQGRFNLSDASENFMKFEQVRIPMMRTDERGRE
                     RAVDTRSFVSDLRKYLNELGVPSKLYLRGLGEAWLILGEK"
     gene            702..1673
                     /locus_tag="PK976_03070"
     CDS             702..1673
                     /locus_tag="PK976_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66900.1"
                     /translation="MKKRIIFFIIAFGFIYNLNAQNYLGKTDDLGRLAIAAIVPNQAE
                     GIPLSAHQLLVNKMEQIVVQNGLGATPVNPRFCMIPLINVLEKEITPTAPPMQALTLD
                     ITFYIVDALSQNIFSQTSIQVKGVGQTEDKAYIQAIRGINVKAGQFKGFIERGKEKII
                     EFYNSQCDVVLKNAQALAGQKQYEEALFVLLSVPDVCRECYDKCMDLSIDIYKQYADY
                     KCTEYLSGARASWANMDVDRAAEYLGKITPDMQCYKEASQLADQITQKQIADGANVWQ
                     FKMKKYDDSIEKDKMMIQAVKEVAKSWAYWGAAPYFNWDWSWIYIKK"
     gene            1749..2321
                     /locus_tag="PK976_03075"
     CDS             1749..2321
                     /locus_tag="PK976_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66901.1"
                     /translation="MKSTILVAGAFLFAVTLAGCKSSKKTVDSTFGKEVIVPCSDEEF
                     HTDSKYFRGTGNGISIDLSTARNKAKMDANTNLAQSINTTLKSVSERYVNERQFDASS
                     EFEQKFEQLTREVVNQQLNNVEVACYKTTQMKDGKFQVFTAVQVSKDEVLNNIANRVS
                     KDKKLQVDYDKMKFEQVFNEEMKKLESERP"
BASE COUNT          803 a          478 c          476 g          600 t
ORIGIN      
        1 atgccttgaa acgggttgcc aaagccgata tcattctcga tctcgatttc gatattaaac
       61 gccaaggacc tcaaaaatat atcgttttca acctcaaagg tctggatgct tatactgcca
      121 agcaagtaac cggtgtagca ggagcaggaa atccttcaag tgcggcatca cccgaacttc
      181 tgctcgaaga agccgtgtta agccacatgg ataattttaa tgctggcctg atgcgccatt
      241 tcgatgacat gtttgccaat ggccgtgaag tgaaagtgat ggtcaaagtt tgggctaact
      301 ggggacaaaa ccttgaatca gaatttggcc ccgattcaaa agaattatcc gaaatcatcg
      361 aagattggtt cttcgataac tgcgtccaag gccgctttaa tctctccgat gcgagtgaaa
      421 acttcatgaa atttgaacaa gtgagaattc ctatgatgcg aaccgatgaa agaggccgtg
      481 aacgtgctgt agatactcgt tcgttcgtga gcgatttacg taaatatctc aacgaattgg
      541 gcgttccttc caaactttat ctcagaggct taggagaagc atggctcata ttaggtgaga
      601 aataaaaaat aaaaataaac ctacttgaaa atgctccaaa ataaaaaagg cttaatcagg
      661 taggtttttg aactcttttt aggataacaa atcatgatac gatgaaaaaa cgaataatct
      721 tttttataat agcattcggg ttcatttata acctgaatgc gcaaaattat ctggggaaga
      781 ctgacgattt gggcagattg gcgattgcag ctattgttcc caatcaggca gaaggaatac
      841 ccctatcggc tcatcagctt ctagtaaaca aaatggaaca aattgtcgtc caaaatggac
      901 taggcgctac gccggttaac cccagatttt gtatgatccc tctaatcaat gtactcgaaa
      961 aggaaattac acctacggca ccccctatgc aagcccttac actcgatatt actttctaca
     1021 tcgtggatgc acttagccag aatatcttca gccaaaccag tattcaggtg aaaggagtag
     1081 ggcaaaccga agacaaagct tatatacaag ccattcgtgg aattaatgta aaagcaggtc
     1141 aattcaaagg ttttatagaa agaggcaagg aaaaaattat cgaattctat aattcacagt
     1201 gtgacgttgt acttaaaaat gctcaagctt tagcaggaca aaagcaatac gaggaagctc
     1261 tctttgttct cctctccgtt cctgatgttt gccgtgaatg ctatgacaaa tgtatggacc
     1321 tttccatcga catctacaaa caatatgctg actacaagtg caccgagtat ctctcgggtg
     1381 caagagcctc ctgggctaat atggacgtgg acagagccgc cgaatacctc ggaaaaataa
     1441 cacccgatat gcaatgctac aaagaagctt cacaattagc cgaccagata acccagaaac
     1501 aaattgccga tggtgctaat gtttggcagt tcaaaatgaa aaaatacgac gactctatcg
     1561 aaaaagacaa aatgatgatt caggccgtaa aggaagttgc taaaagctgg gcttattggg
     1621 gtgcagcacc ctattttaac tgggattgga gctggattta catcaaaaaa taaccttaat
     1681 aaacctttat taaataaatc aattaattta tcaaccaaca tttcaaatta aacttaacaa
     1741 ctatgaagat gaaaagtacg attttagtag ccggtgcatt tctttttgca gtaacgctgg
     1801 caggatgcaa gagttccaaa aaaacagtag attctacatt tggtaaggaa gttatcgttc
     1861 cttgcagtga tgaagaattt cataccgatt ctaaatactt caggggtaca ggcaatggca
     1921 tcagtattga tttaagtact gcacgcaaca aagccaaaat ggatgccaac accaatttag
     1981 cccaaagcat caatactacc cttaaatccg tgtccgaaag atatgtaaac gaacgccaat
     2041 tcgatgcaag ttcggagttc gaacaaaaat tcgaacaact cacccgcgaa gtagttaatc
     2101 agcaattgaa caatgtggag gttgcttgct acaaaaccac ccaaatgaaa gacggaaagt
     2161 ttcaagtgtt caccgcagtg caggtatcta aagatgaggt tctgaacaac attgccaata
     2221 gagtttcgaa ggataaaaaa cttcaggttg actacgacaa aatgaaattc gaacaagtct
     2281 ttaacgaaga aatgaaaaaa ctcgaaagcg aacgtccctg aataatttca ttcaccgaaa
     2341 atcagcagct cattccg
//
LOCUS       DAORPD010000193         2356 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_8177_length_2356_cov_2.398088, whole genome shotgun sequence.
ACCESSION   DAORPD010000193 DAORPD010000000
VERSION     DAORPD010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8177_length_2356_cov_2.398088"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="PK976_03080"
     CDS             <1..312
                     /locus_tag="PK976_03080"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC66902.1"
                     /translation="IRPIVGKPLAGVSKDKGNSKNLAVFPNPVIENVIYLSGELLLNG
                     INENFTLSLFNSNGQLVFRSYLKPRLDLPPSLSNGIYILQIRGKNGDIVYTTKLAVSK
                     I"
     gene            316..1347
                     /locus_tag="PK976_03085"
     CDS             316..1347
                     /locus_tag="PK976_03085"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRC66903.1"
                     /translation="MNPEQDFNEDLFEHYRIVVDKGQSLLRIDKFLVKRVENASRTKI
                     QNAARAGNILVNHRTVKPNYKVKPGDVISILLPGEPREIENIPEDIPLNIVYEDEDVL
                     IVNKPSGMVVHPAYGNYTGTLFNALLFLFMGKTNKDGKEIYPWLVHRIDKDTSGLLII
                     AKNELAQAILAHQFYEHSIKREYTALIWGDLENETGTIEGYINRNPKDRRIMTIYQDP
                     EKGRTSITHYNVIKRWGYVTLINCSLETGRTHQIRAHFASIGHPLFGDTPYGGRQILK
                     GSLYSKYKQFVENSFGLLKGQALHARTIEFVHPSSHQKVYFESELPADFQEVIERWDK
                     YVLGRNLQS"
     gene            1456..>2356
                     /locus_tag="PK976_03090"
     CDS             1456..>2356
                     /locus_tag="PK976_03090"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HRC66904.1"
                     /translation="MHTTRYIFVTGGVTSSLGKGIVSASLAKLLQARGYKVTIQKLDP
                     YINVDPGTLNPYEHGECYVTEDGAETDLDLGHYERFSNEPTSQANNVTTGRIYQAVIE
                     KERRGDYLGSTVQVIPHITDEIKYRIKLLSKYKDYDFIITEIGGTVGDIESLPYIETV
                     RQMRWEMGNLCVVIHLTLVPYLSSTGELKTKPTQHSVKTLLEQGVQPDIIVCRTEKPL
                     NEALRNKIALFCNVSSQAVIESIDMESIYEVPLKLRDEGLDREVLRKCGLPFEGQPDL
                     KSWEEFLYKMKNPKYSVEIGLVGK"
BASE COUNT          761 a          458 c          494 g          643 t
ORIGIN      
        1 attaggccca ttgttgggaa accacttgca ggtgtatcaa aagataaggg aaattcaaag
       61 aatcttgcag tatttcccaa tccggtcata gaaaatgtga tttatttaag tggtgagctg
      121 ttgctaaacg gtataaatga gaattttacc ctcagtctgt tcaattctaa cggacaactt
      181 gtttttcgaa gttacttgaa accccgactc gacctccctc cttccctttc gaatggtata
      241 tatattcttc aaatcagagg aaagaacggg gacatcgttt acacgaccaa actggcagtt
      301 tctaaaattt aaaccatgaa tcctgaacag gattttaatg aggatttatt cgaacattac
      361 cggatagtcg tcgataaagg tcaatcctta ttacgcatag ataagttcct tgtgaagagg
      421 gtggaaaatg cttcgcggac taaaattcaa aatgcagcca gggcagggaa tattctggta
      481 aaccatagaa ccgtaaaacc taattacaaa gtaaaaccag gcgatgttat cagcatttta
      541 ttaccgggcg aacctcgtga aatagagaat attcctgaag atatacctct gaatattgtt
      601 tatgaagatg aagacgtttt gatagtgaac aaaccttcgg gcatggtggt tcatcctgcc
      661 tatggtaatt ataccggaac gcttttcaat gcgttgttgt tcctttttat gggaaaaacc
      721 aataaagatg gaaaggaaat atacccatgg ttggttcatc gtatcgataa agatacttcg
      781 ggattattga tcattgcaaa gaatgaattg gcacaagcca tacttgctca tcaattctac
      841 gaacattcga tcaaaaggga atataccgct ttgatttggg gagatctgga aaatgaaaca
      901 ggaacaatag aaggatatat caaccgcaac ccgaaagatc gtaggataat gaccatttat
      961 caggatcctg agaaaggaag gacaagtatc acgcattata acgtcatcaa aagatggggt
     1021 tatgtaactt taataaattg cagtcttgaa acaggccgta ctcatcagat aagagcccat
     1081 tttgcatcta ttgggcatcc tctttttgga gatactccct atggaggccg ccaaatcctc
     1141 aaaggttcat tgtattcaaa atataaacaa ttcgtcgaaa atagtttcgg tttgttaaag
     1201 gggcaagcgc ttcacgcccg aaccatagaa tttgtccatc cctcaagcca tcaaaaggtt
     1261 tatttcgaat cggaattgcc tgccgatttt caggaagtaa tcgaaagatg ggataaatat
     1321 gtactgggaa gaaatcttca gtcataatat aattatttga ataataatgc gataactaaa
     1381 atttagggat tttcgaaaat ctatttgaaa tttgttgtaa cttgccgccc cgtaacgtaa
     1441 ctaaaaaagt taactatgca tacaacacga tatatattcg ttacgggagg tgttacctct
     1501 tctctgggaa aaggaatagt atcggcttca ttggctaaat tactgcaggc aagaggttat
     1561 aaagtaacta ttcagaaact cgacccctat atcaatgtcg atccgggaac actcaatccg
     1621 tatgaacatg gcgaatgtta tgtaaccgag gacggggctg aaaccgatct cgaccttggc
     1681 cattacgagc gtttttcgaa cgaacccacc tctcaggcaa ataatgtgac caccggaaga
     1741 atctatcaag cagtaatcga aaaggaaaga cgaggagact atttgggttc taccgtgcag
     1801 gttatcccgc atataaccga cgaaattaaa taccgtatca aactgctttc caaatataag
     1861 gattacgact tcattatcac tgagatagga ggtacggtag gcgacataga gtcgctgcct
     1921 tatatcgaaa ctgtacgtca gatgcgctgg gaaatgggta atctctgtgt agtgattcat
     1981 cttacactgg ttccttatct ctcttcgacg ggggaactga agacaaaacc tacccaacat
     2041 tccgtaaaga ctttactcga acaaggagtc caacccgaca tcattgtctg ccgtaccgaa
     2101 aaacctctca atgaggcttt gcgcaataag attgcactgt tttgcaatgt ttcttctcaa
     2161 gccgtaatcg aatccataga tatggaatcc atttatgaag tcccattgaa attaagagac
     2221 gaaggcctcg acagagaagt actgcgtaaa tgtggacttc cgttcgaggg gcaacccgac
     2281 ctcaagagct gggaagagtt tctttataag atgaagaacc ccaaatattc tgtggaaatt
     2341 gggctggttg ggaaat
//
LOCUS       DAORPD010000194         2346 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_8232_length_2346_cov_2.051069, whole genome shotgun sequence.
ACCESSION   DAORPD010000194 DAORPD010000000
VERSION     DAORPD010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8232_length_2346_cov_2.051069"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="PK976_03095"
     CDS             <1..182
                     /locus_tag="PK976_03095"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC66905.1"
                     /translation="AGSEVKLYSVFGQVVGITLQINPGETSNVRFPAYIRTGMYLLRC
                     NQPSKGTEVVKIVIY"
     gene            complement(548..1753)
                     /locus_tag="PK976_03100"
     CDS             complement(548..1753)
                     /locus_tag="PK976_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRC66906.1"
                     /translation="MPEISEKGKKMPASPIRKLVPFADQAKQRGIQVFHLNIGQPDIE
                     TPQTILDAIHHFDQKVIEYSHSAGFLSYRTKLTQYYAKLGISIQPDDIIITTGGSEAI
                     LFALQSTMDPGDEIIIPEPFYANYNGFAINSGVIVKPIPSFIENGFALPAISEFEKAI
                     GPRTKAIMICNPNNPTGYLYSPEELELLRDIALKYNLYLFADEVYREFCYDGREHFSI
                     MQLEGLDQHAVLIDSVSKRYSACGIRIGCIISKNKQLMATAMKFAQARLSPPSIGQVA
                     AEAALDTPAQYFENVRNEYFARRNVVVEAINKMPGAFCTSPQGAFYVMARLPIDDSDL
                     FCKWLLEDFESKGRTVMIAPGTGFYSTPGKGIDEVRISYVLRVDELQQAMECLAEALT
                     IYPHRKHCK"
     gene            complement(1913..>2346)
                     /locus_tag="PK976_03105"
     CDS             complement(1913..>2346)
                     /locus_tag="PK976_03105"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HRC66907.1"
                     /translation="QAQDAKPVSKNGPIITFDQKLRDEAGNIVWDYGTIYKDSKGDSY
                     FRFTNTGKEPLVLSEVRSSCGCTVPSWPREPILPGKSDTIKVRYATNRLGVINKTITV
                     MSNAVNNPVILRIKGNVIEKPAEVVPEKRSVDAAQPLNKAK"
BASE COUNT          686 a          514 c          455 g          691 t
ORIGIN      
        1 aagcaggctc tgaagtcaaa ttatattccg tgtttggaca agtcgtcggc ataactttgc
       61 aaataaaccc gggcgaaact tcaaatgtca ggttcccggc atacatcaga accggaatgt
      121 acttgctacg ctgcaatcaa ccatccaaag gaaccgaagt agttaagatc gtcatttatt
      181 gactggctca atgctgaaaa ccgatggctt tcccaaggag aacttattcg aatttaaaat
      241 gcttcacatg aatgctgaga atgtcaaatc cggataaatt tgttcgaact acatcttttg
      301 aaaagcaaaa atcatccttg attctttatt atccgaacat agaatcttct acttgaattt
      361 ccttgagcct atgttcaatc attatttccg atgaaatttg cttcatctaa tcaatttttc
      421 tcaccgacag catcatcaat aaattttgtt catttttacc tttttaaaag gtataaccaa
      481 taaaaacatc aagctggttc aatggatatt tttccaaaaa tccagctcaa gttcaagaaa
      541 aaaaatatca tttgcaatgc tttcggtggg ggtaaattgt caaggcttct gccaggcatt
      601 ccatagcctg ttgtagttca tcaactctaa gcacatacga gattctgacc tcatcgattc
      661 ctttacctgg agtagaataa aaacccgtac ccggcgctat catcaccgta cgtcctttcg
      721 attcaaaatc ttcgagaagc catttgcaaa agaggtcact atcatcaatc ggtaatcgag
      781 ccattacata aaaagctccc tgagggctgg tacaaaatgc gcctggcatt ttattgatgg
      841 cttcgactac cacattgcgt cgggcaaaat attcattcct gacattttca aaatattggg
      901 ctggtgtatc gagcgcagct tcggcggcta cctgaccgat acttggaggg ctcaatctgg
      961 cctgagcaaa tttcatggca gtagccatca attgcttatt cttcgaaatt atgcatccta
     1021 tccttattcc acaggcactg taacgtttgc tgaccgaatc gatcaaaacg gcatgctgat
     1081 ctaatccttc cagctgcata atactgaaat gttcacgtcc atcataacaa aattccctgt
     1141 acacttcatc ggcaaaaaga tataagttgt acttcaaggc aatgtctcgg agtaattcaa
     1201 gctcttcggg actatataag taacctgtag ggttattcgg attgcaaatc ataatggcct
     1261 tggtacgggg tccaattgcc ttttcgaact cactgatggc aggaagggca aaaccatttt
     1321 cgataaagga agggataggt tttacgatga ctcccgaatt aatggcaaaa ccattgtaat
     1381 tggcatagaa tggctcggga attataattt catctccagg atccatggta gattgcagag
     1441 caaataagat tgcttccgag cctcctgtcg taatgatgat atcatcgggt tggattgaaa
     1501 tacccagctt ggcataatac tgagtgagct tggtccggta cgacaaaaat ccggctgaat
     1561 ggctatattc gatgaccttc tggtcgaaat ggtgaatagc atccaagatt gtttggggtg
     1621 tttcgatatc gggttgacca atattcagat gaaacacctg gatacctctc tgtttggcct
     1681 gatcggcaaa tggaacgagt ttgcgaatgg gagaagctgg catcttcttg cctttctctg
     1741 atatctctgg catagcaatg aatttaaagt taagtaaccg caaaggtaaa caaatactac
     1801 cattttaaat tttacagaca acgcaacatc tattgaaatc tcctcaaacc tacacccaca
     1861 aaaaaaccca gaggtacatt ctccgggcat gagtattctt caagattaga gtttatttgg
     1921 ctttattcaa aggttgagca gcatcgaccg acctcttctc tggaactact tcggcaggct
     1981 tttcgattac attgcccttg atgcgtaaaa ttactggatt attcactgca ttcgacatca
     2041 ccgtaatagt cttattgatt actcctaaac gatttgtagc ataccttacc tttatggtat
     2101 cacttttacc cgggagtatg ggttcacggg gccagctggg cacagtacat ccacacgaag
     2161 aacgtacttc ggaaagcacc agaggctctt taccggtatt ggtaaaccgg aaataagaat
     2221 ctcctttgct atccttatat attgtaccat aatcccatac tatattccct gcttcatcac
     2281 gcagtttttg atcgaaagta ataatgggac catttttgct gactggtttc gcatcctgcg
     2341 cctgta
//
LOCUS       DAORPD010000195         2341 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_8252_length_2341_cov_3.327209, whole genome shotgun sequence.
ACCESSION   DAORPD010000195 DAORPD010000000
VERSION     DAORPD010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8252_length_2341_cov_3.327209"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..715)
                     /locus_tag="PK976_03110"
     CDS             complement(<1..715)
                     /locus_tag="PK976_03110"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HRC66908.1"
                     /translation="MQRLQYFKVQMLNALRLQFSLRNGKPMALLLIAVILSASLLKSQ
                     PPAGYYDGTEGLTGVALQAKLHDIIDDHASVSYGSILLHFQTTDKKPNNTVWDMYSDV
                     PGGNPPYIYYFNEDECGSYKSEGDCFNREHSWPASWFNDLIPMYTDLFHIYPTDRYVN
                     GKRSNYPYGEVGSASWTSMNGSKLGNCNYPGYSQVVFEPIDEYKGDLARSYFYVATRY
                     YNEDNGWASNGMVTGSQLKP"
     gene            complement(990..1373)
                     /locus_tag="PK976_03115"
     CDS             complement(990..1373)
                     /locus_tag="PK976_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66909.1"
                     /translation="MSIPKERWKLRPNIEATAREFKIRTEDGKLKVRGLIKASLFAYS
                     SGIAINFGRTYRFLVKNGCPDGFNRPIIVFLKDIMSNTGEILTKCEFIVDNFSLFRYF
                     WFFLIQKIFSGPKMSKQETLIQLSF"
     gene            complement(1599..>2341)
                     /locus_tag="PK976_03120"
     CDS             complement(1599..>2341)
                     /locus_tag="PK976_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66910.1"
                     /translation="DARAVKGIVDKYKYSKERFYFGFFQYQKIFSFTIAHRSIDAIKQ
                     DAKEGRQGIYRPTDRTLFKKRADKYIYGLKSSELPHELEKLGKIYYTIFEWLSKKEEY
                     HSRKEYINFTRAYNEHFVVKGEEVNPRSEKELTRSMLQSPDDVDATYRKKNGEQSKGF
                     SINITETANPDNPIQLITGIVVMPNNVDDSQILNERIDIIKEKTPQLNEMHIDGDFGS
                     EENDKKFEELGINHITTAVRGREREIEI"
BASE COUNT          669 a          484 c          348 g          840 t
ORIGIN      
        1 atggtttcaa ctgcgagccc gttaccattc cattgcttgc ccagccattg tcttcattgt
       61 agtaacgtgt tgccacataa aaatagcttc ttgccaaatc gcccttgtat tcatctatag
      121 gctcgaacac cacttgggaa tatcctggat aattacagtt ccctaattta cttccattca
      181 tagaagtcca tgaggcgctt cctacttcac cataagggta attgcttcgt tttccgttta
      241 cgtaacgatc ggtgggataa atatgaaaga gatctgtgta catgggaatt aaatcattaa
      301 accagcttgc aggccatgaa tgttccctgt tgaaacaatc cccttccgat ttgtagctac
      361 cacattcatc ttcattgaaa taataaatgt agggaggatt tccacccggt acgtcggaat
      421 acatatccca aaccgtgtta ttgggttttt tatcggtggt ttggaagtgc aacaaaatcg
      481 aaccatagga taccgaagcg tgatcgtcga taatatcgtg cagcttagct tgaagagcca
      541 cgcctgtcag gccttcggta ccatcgtaat atcccgccgg aggttgcgat tttagcaaac
      601 tggccgaaag tatgactgca ataagcaaaa gagccatagg cttgccgttt cttagcgaga
      661 attgtaaacg taaggcgttc aacatctgaa ctttaaaata ttgaagtctt tgcaaagata
      721 agtcagtttt taggattcaa atcttttcac atctatgttc gaaaattttg aaatagatga
      781 aatttttaag ataatttttt gtatatttac attggtaatc atttacaaac gaaagaagtc
      841 aaaataaaaa ttcattgatt tataaatctt taacctaaat ccaattactt atgaaaaaat
      901 tagtatttat aacgacgcta ttcttctcca tcccttaaaa gcttcttatt atgaatcgaa
      961 ttttgttatt tttgattatt gagtccggtt taaaaactaa gttggattaa agtttcctgt
     1021 ttcgacattt ttggacctga aaaaattttt tgaatcaaga agaaccaaaa atacctaaac
     1081 aaggaaaaat tatcaacaat aaattcacat tttgttaata tttcccctgt atttgacata
     1141 atatccttca aaaatacaat tattggccta ttaaatccat caggacatcc atttttgacc
     1201 agaaatctgt atgtccttcc aaaattaatc gctatcccac tgctataagc aaataaactc
     1261 gcctttatta atcctcttac ttttaatttc ccatcttctg tccttatctt aaattcacgt
     1321 gccgtagctt ctatatttgg cctaagtttc cagcgctctt taggtatgct caaaatattg
     1381 cgactgcgtt tgttttgtag atactctttt atgtgttaaa taatatcacc cattgttctt
     1441 aaatgcctaa caattatctt ttaacaaaca tcccttgcat attttcatag caaaacatac
     1501 tttatgtcgt ttctttgtgg gcatggactc taccttctga aaaggacatt ctacagtata
     1561 cacttccggc gtcttggatt tctgctctat ctttatttct atatttctat ctctctttct
     1621 ctccctctaa ctgctgttgt gatatggttt atccctaatt cttcaaactt cttatcattc
     1681 tcttcactac caaaatcacc atctatgtgc atttcattca actgtggcgt cttctcttta
     1741 attatatcga tcctttcgtt taatatctgg ctgtcatcaa cattgttggg cataaccact
     1801 atgccagtga ttaattgtat gggattatct ggatttgcag tttccgtaat gtttatcgaa
     1861 aatcctttac tttgctcccc attctttttc ctgtaagtag catccacatc atcaggcgat
     1921 tgcaacatgc tacgggtcaa ctccttctca gaccttggat tcacttcctc tcccttcaca
     1981 acaaaatgtt cattgtatgc ccgagtgaaa ttgatatatt ctttccttga atgatattcc
     2041 tcttttttac tcaaccattc gaatatagta tagtatatct tacctaactt ttctaattca
     2101 tgaggtaatt cactggactt caacccataa atgtatttat ctgccctttt tttaaataag
     2161 gttctatctg ttggccgata aattccttgt cttccttctt tagcatcctg tttaatcgca
     2221 tcaatacttc tatgagcaat tgtaaatgag aatatttttt gatattggaa gaaaccaaag
     2281 tagaatcgct cctttgaata tttgtattta tcaacaattc ctttaactgc tcttgcgtca
     2341 g
//
LOCUS       DAORPD010000196         2330 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_8309_length_2330_cov_2.346374, whole genome shotgun sequence.
ACCESSION   DAORPD010000196 DAORPD010000000
VERSION     DAORPD010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8309_length_2330_cov_2.346374"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="PK976_03125"
     CDS             complement(<1..295)
                     /locus_tag="PK976_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66911.1"
                     /translation="MNLKRRMKAIPGIFTMILMMKGGIAFSSSSSACMFMPPVDSNKI
                     VLKDTLHFADSLEDTDSEEIDDLIASNWIVNQIFAYRDVKEIPLNICIQLVDSL"
     gene            complement(621..1391)
                     /locus_tag="PK976_03130"
     CDS             complement(621..1391)
                     /locus_tag="PK976_03130"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HRC66912.1"
                     /translation="MENAVCQLSAAPLRVEPLHKAEMISQLLFGEPVEIIERRPPWCL
                     IKSKIDYYEGWVDAQQLEIMPQTPQFNEDNLLICHQLLSSLIHCITGEKQLITFGTML
                     TAENGKVKVGSSEYYKHDDFMPFFRKTTSNDIINSAKLWLNTPYLWGGKTPLGVDCSG
                     FVQLVFRCNGQKLPRDAYQQAELGIDIDFVSESMPGDLAFFSNDESHISHVGILIDNS
                     RIIHASGKVRIDAFDHQGIYNLSLKTYTHQLRLMKRLL"
     gene            complement(1447..>2330)
                     /locus_tag="PK976_03135"
     CDS             complement(1447..>2330)
                     /locus_tag="PK976_03135"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC66913.1"
                     /translation="GATKENLKQWQFFFNYEAANCSQPALEPELKPSVGGTKKAASDV
                     SISNLGSDFYLLTLNQGIPENVNAFFSGWSRLDYPSASGVCIHHPQGDIKKISTYTTP
                     TLSSSWENMSNSHWKVSWSATENGHGVTEGGSSGSALFNSNGLLMGTLTGGQSSCDSS
                     NLNQPDYFGKFSAHWDLNGQADTLRLQPFLDPDHIGMTTLEGMTLGLDQTFDILIFSV
                     FPNPSSQNISLHLPDSFSTLKIMVEIFHPTGQKVMKIELDGIHPVPIQISHLCPGIYF
                     IRITSDEKSSIAKFIKQ"
BASE COUNT          703 a          441 c          463 g          723 t
ORIGIN      
        1 gcagtgaatc gaccaattga atacaaatat tgagcggaat ttcttttaca tctcgatagg
       61 caaaaatttg attcacaatc caattgcttg caatcaaatc atcaatttct tctgaatcgg
      121 tatcttcgag tgaatcagca aaatgtaaag tatctttcaa cacaatcttg ttagaatcta
      181 caggcggcat aaacatacag gccgaagaac tcgacgaaaa tgcaatccct cctttcatca
      241 tcaagatcat tgtaaatatt ccggggatag ctttcattct tcttttaagg ttcatcagtc
      301 agtatttttt tattcgagtg attttttagt atgtgatcgt tttataaaaa tttttcgcaa
      361 agtacgaatt tttttaaaat ccaaaacatt tcaaaatatt ttagccttag aatatatctt
      421 tcggaattgt tgataaccga aatttggtaa attttcgaga ggcggctgta ctgttctcgt
      481 tttacaacga tatcgaattt aacaatagat ttaaatgtaa aatggatgtt ctggctcatt
      541 tcaatggcat aaagcgtatg aatggataaa tttcccaaat gaggaaaatc gatgttgtta
      601 agcgaatgag catcaaaatt ttataaaagt cgtttcatca atctcaattg atgtgtatag
      661 gtttttaatg ataagttata gattccctga tgatcgaaag catctattcg aactttgccc
      721 gaggcatgaa taattcgact gttgtcgata agaatcccca catgcgaaat atggctttcg
      781 tcattggaga aaaaggctaa gtcgccaggc atggattcgg aaacgaaatc tatatcaata
      841 cccaattcgg cctgttgata agcatcgcga ggtaattttt gtccattgca tcggaaaacc
      901 agttgaacga aacctgagca atctactcct aaaggtgttt ttccacccca taagtagggg
      961 gtattcagcc ataattttgc cgaatttata atatcgttgc tcgtcgtttt tctaaaaaaa
     1021 ggcatgaaat cgtcatgttt atagtattct gaactcccca ctttaacctt gccattttct
     1081 gcggtaagca ttgttccaaa agtgatgagt tgtttttcac ctgttatgca atgaatgaga
     1141 gaagaaagca attgatggca tattaacaga ttatcttcgt taaattgtgg agtttggggc
     1201 ataatttcca attgttgtgc atctacccat ccttcgtaat aatcaatttt actttttatc
     1261 aaacaccatg gcggacgtct ttcgattatt tcaaccggtt cgccaaagag caactgcgaa
     1321 atcatctcgg ctttatgcaa aggctctacc ctcaggggtg cagcactaag ctggcaaact
     1381 gcattttcca tcttcaaaaa acaatgtcat agttgaaaca tacatgtgaa taagcgtaaa
     1441 ttttggctac tgtttgataa attttgcaat cgaacttttt tcgtccgaag taatacggat
     1501 aaaatagata cccgggcata aatggctaat ctgaatagga acgggatgaa ttccatccaa
     1561 ttctattttc attacctttt gacctgtagg atgaaaaatt tccaccatta ttttcaaggt
     1621 cgaaaagctg tcgggaagat gtaaagagat attctgactg gaaggattgg gaaagacaga
     1681 gaaaattagg atatcgaagg tttggtctaa tcctaaggtc attccttcga gggtggtcat
     1741 tccaatatga tcgggatcga gaaagggttg gagtcgcaaa gtatcggctt ggccattcaa
     1801 atcccagtgt gcagagaatt ttccgaaata atcgggttga ttgaggttgg aggagtcgca
     1861 agaagactgg cctccggtta aagtacccat taataggcca ttactgttga acaaggcgct
     1921 tcccgaagag cctccttcgg ttacgccatg accattttcg gttgccgacc agctcacctt
     1981 ccaatgagaa ttactcatgt tttcccagct cgatgatagg gtaggtgtgg tataggttga
     2041 tatttttttg atgtcgcctt gcggatggtg aatgcaaacc cctgatgcgg aagggtaatc
     2101 caaccttgac caaccagaaa agaaggcatt cacattttca gggataccct gattgagagt
     2161 caataaataa aaatcgctcc ccaaatttga aatgctcaca tcggaagccg cctttttcgt
     2221 tcctcccaca gaaggtttca actctggctc taatgcaggt tgcgaacaat ttgcagcctc
     2281 gtagttaaag aaaaactgcc attgtttcag attttctttc gtggctcctt
//
LOCUS       DAORPD010000197         2324 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_8335_length_2324_cov_2.659762, whole genome shotgun sequence.
ACCESSION   DAORPD010000197 DAORPD010000000
VERSION     DAORPD010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8335_length_2324_cov_2.659762"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="PK976_03140"
     CDS             complement(<1..284)
                     /locus_tag="PK976_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66914.1"
                     /translation="MKRFLLTIFIACSVSIAWSQTAKRTTAYNFLRNGKLDKALENIE
                     PCLTHPKTMNEAKTWFYRGNIYLQIGLSEKPEYKALSDNPFQIAYESYQKA"
     gene            complement(369..>2324)
                     /locus_tag="PK976_03145"
     CDS             complement(369..>2324)
                     /locus_tag="PK976_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007214238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HRC66915.1"
                     /translation="LMNYIKGPDFPTGGIIYGYDGVREAYETGRGRIILRGTADIEED
                     NDRERIVVTSIPYQVNKAEMIKRTADLVNEKKIDGISDIRDESDREGLRIVYELKREA
                     NANVVLNQLYQHTPLQTSFNVNNIALVKGRPFTLNLKELIVYFIDHRHNVVIRRTKFQ
                     LNEAEKRAHVLEGLLIALDHIDEVIALIKSSADTEEAKNGLMEKFGLTEIQAKAILAM
                     RLSTLTGLEREKLQQEYQELRRMIDYYKEILSNESIRMDIIKKELFELKEKYGDEPKT
                     EIEYTSQDFRVEDTIADDSVVVTISHLGYIKRTPLREYRRQKRGGQGAKGSDIREEDF
                     VEHLFVATNHNYLLLFTEKGKCFWLRVFEIPEGVKTAKGRPIQNLINLESDDKIRAYI
                     NLKNLSDPEYINNNYIILCTRKGIIKKTSLEAYSRPRINGINAINIREGDHLLEARLT
                     DGQSEIIMALRSGRAIRFSETKVRPMGRTAMGVRGVRLAHKNDEVIGMICLDSHDYDI
                     LVVSEKGYGKRSSLDDYRETNRGGKGVKTLNITDKTGDLIAIINVKDSDDLMIINKNG
                     ILIRIPVAELRIQGRATQGVKLINLKGNDEIAAVTRVEPEEEDKNEEEEETSRIHTAI
                     DEMPTPEPEFESGPIESIEDEDNTAED"
BASE COUNT          569 a          505 c          442 g          808 t
ORIGIN      
        1 gctttctgat aactttcgta agctatctga aatggattat cgcttaatgc tttgtattcg
       61 ggtttttcgc tcaatccaat ttgaaggtag atgtttccac gataaaacca tgtctttgct
      121 tcattcatgg tttttggatg ggtcaaacag ggttcgatgt tttcgagagc tttatcgagc
      181 ttgccattgc gaagaaaatt gtaagctgtc gttcttttgg cagtttgact ccaagcaata
      241 cttacactgc aagctataaa aatcgtcaaa aggaatcttt tcatggtaat taagaatttt
      301 gtatcaattt tcctctgaca aaattatgaa ttttattcat ttcaaatcaa atcgtagaat
      361 tagaaaaatt aatcctcagc agtattgtct tcatcttcta tcgattctat aggacctgat
      421 tcgaattcag gttcgggggt tggcatttca tctattgctg tgtgtatcct cgaggtttct
      481 tcttcttctt cgtttttatc ttcttcttcg ggttcaacgc gggttactgc agcaatttcg
      541 tcgtttccct tcagattgat gagtttcacg ccttgagtag ctctgccttg tattctcaat
      601 tcagcaacag gaatgcgtat aagtatgcca tttttattga tgatcatcag gtcgtcggaa
      661 tctttgacat tgataatagc gatcaagtct cccgttttat cggtgatatt cagtgtttta
      721 acgcctttgc cacctcggtt agtttcccta taatcgtcaa ggctcgatcg ttttccataa
      781 cccttttccg atacgaccag aatatcataa tcatggctat cgaggcaaat cattccgatt
      841 acttcgtcat ttttgtgagc cagtcgaaca cctctcactc ccatagccgt gcggcccatt
      901 ggccttactt ttgtttcgga aaatcgaatg gcacgacccg aacgcaaggc catgataatt
      961 tcactttgcc cgtcggtaag tctggcttcc aacagatgat caccttccct gatgttgatg
     1021 gcattgatac cattgattcg tgggcgggaa taggcttcga gcgaggtttt cttgataatg
     1081 ccctttcttg tacataaaat gatgtaattg ttgttaatat attcagggtc ggagagattt
     1141 ttcagattaa tgtaagccct tattttatcg tcagattcca gatttatcag attctgaata
     1201 ggcctgccct ttgctgtttt tactccctcc gggatctcga aaacccttaa ccagaaacat
     1261 tttccttttt cggtaaacag caataaatag ttatgattgg tagcaacaaa caaatgctcc
     1321 acaaaatctt cttcacgaat atcacttcct ttggctccct gtcctcccct tttttggcgt
     1381 ctgtattctc gcaaaggagt tcgtttgata taccccagat gggatatggt tactactacg
     1441 ctgtcgtcgg ctatggtatc ttctactcta aaatcctggg aagtgtactc tatttcggtt
     1501 ttaggttcgt ctccatattt ttccttcaac tcgaacagtt ccttctttat aatatccatg
     1561 cgaatgcttt cgttcgagag gatttccttg taataatcga tcatgcggcg tagttcctga
     1621 tattcttgtt gaagtttttc gcgttccaaa ccggtgagag tcgaaagtct catggcaagg
     1681 atagctttgg cctgtatctc agttagcccg aatttttcca taagtccatt tttagcctct
     1741 tcggtatcgg ccgaactttt aataagggca attacttcat cgatgtgatc gagagcaatg
     1801 agcaaacctt caaggacgtg tgcacgtttt tcggcttcat tcaactggaa tttcgttctt
     1861 cttatcacta cattatgacg atgatcgata aaatagacaa taagttcctt aaggttcaag
     1921 gtaaaaggtc tgcccttgac cagtgcaata ttatttacat tgaacgaagt ctgaagtgga
     1981 gtatgctgat ataattgatt gagaaccaca ttggcattag cttcacgttt gagttcatat
     2041 acaatacgca atccttcgcg atccgattcg tcgcgtatat cgctaattcc atctattttc
     2101 ttttcgttaa ccaaatctgc agttctcttg atcatttcgg ccttatttac ctgataaggg
     2161 atagatgtaa ccacgatgcg ttctctgtca ttgtcctcct cgatgtcggc cgtgcctctc
     2221 aaaatgattc taccccgtcc ggtttcgtaa gcttctctta ctccgtcata accataaata
     2281 attcctccgg taggaaaatc cgggcctttg atatagttca tcag
//
LOCUS       DAORPD010000198         2323 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_8342_length_2323_cov_2.315256, whole genome shotgun sequence.
ACCESSION   DAORPD010000198 DAORPD010000000
VERSION     DAORPD010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8342_length_2323_cov_2.315256"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="PK976_03150"
     CDS             <1..431
                     /locus_tag="PK976_03150"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC66916.1"
                     /translation="GYDRIGATHQYSRIKTAPWVNAPGQWEEGALRWCFYDCNILGDP
                     AMKIWTSNLTSIPYARNNSNNFNIVYSPVNQFIEITSEESSSFQLQLIDFTGRIILTK
                     DNRNQKNIKLDTQTVKNGLYLLHINSNSFHLTKKILIYNL"
     gene            463..>2323
                     /locus_tag="PK976_03155"
     CDS             463..>2323
                     /locus_tag="PK976_03155"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HRC66917.1"
                     /translation="MMKTKFYSLTVYATLIFFMLMQSVGVLAQTQTITLQNSSTKQGF
                     NLKESKSSAVEVQYVVNEFYLEDFTLNGEQMKSVLLSGVFLPNDEGAPNLAGESRFIA
                     IPQGATPVLDIKSVEIEKFQNVEVGPAPRIPLESDDSPLHYEKNPAIYSVNAFYPAQP
                     IKLSEVSKIRGVDVVLLGITPFQYNPVTKELIVYKNIDVEIRFEGGNSQFGENAYRNP
                     YFDAILSDNLLNYSSLSHIDYAARYASYYAPTEDDECEYIIICPDGPEFQTWADSIRK
                     FRTEQGILTKVFTLTQIGGNTTTAIETFINNAYNNWSIKPIACLLLGDYGTNATNSII
                     SPIYNNYCVSDNIYADVDGDMMPDVIFSRITARNTTELQVMVTKVLNYERNPPTDTTF
                     YNRPITALGWQTERWFQICSEVVGGFFKNKLGKNPVRQNAIYQGNPNVDPWSTATNTN
                     TVLNYFGPNGQGYIPATPQELGGWTGGSATGINNAINNGAFIIQHRDHGMETGWGEPS
                     YTNSNINALSNTNLTFVMTINCLTGKYNWGSECFAEKFHRHTKNGLNAGALGLLAPSE
                     TSYSFVNDTYVWGVYDNWWTDFMPSYASTPEPRGVLPSFGNAAGKYFLRQSSWP"
BASE COUNT          711 a          506 c          435 g          671 t
ORIGIN      
        1 agggttacga tcggataggt gctacccacc aatattcacg tattaaaact gctccatggg
       61 tcaatgcccc tggtcaatgg gaggaaggtg cacttcgctg gtgcttttat gactgcaata
      121 tcttaggcga tcctgcaatg aaaatatgga caagcaatct tacttctatt ccttatgccc
      181 gaaacaattc caataatttc aatatcgtct attctcctgt aaaccaattc attgaaatta
      241 cttcagaaga atcgtcgagt tttcaactcc aattaatcga ctttacggga agaattattc
      301 taactaaaga caatagaaat cagaaaaata tcaaattgga tactcaaact gtaaaaaatg
      361 gattatattt gctgcacatt aattcgaatt cttttcacct cacaaagaaa attttgattt
      421 acaacttata actaatgtat taacaatcag tttatttata tcatgatgaa aacaaagttc
      481 tactccctta cagtttatgc aacgctcatc ttctttatgt tgatgcaaag cgttggagtt
      541 ttggcccaga ctcaaaccat tacattacag aattcatcga cgaagcaagg atttaacctg
      601 aaagaatcca aatcaagcgc tgtcgaagtt caatatgtag tgaacgagtt ctatttagag
      661 gattttacac ttaatggcga gcaaatgaaa agcgtattgt tgagcggggt ctttttgccc
      721 aacgacgaag gtgctcccaa tttagctggc gaaagccgct ttattgccat tccacaaggt
      781 gctacaccgg ttcttgatat aaaatcggta gaaatcgaaa aatttcaaaa tgtagaagtt
      841 ggacctgcac cccgtattcc tctggaatct gatgacagtc cattgcatta tgaaaaaaat
      901 ccagccatct attccgtaaa tgcattctat ccggctcaac cgattaaatt atcggaagtt
      961 tcaaaaatta gaggcgtaga tgttgttctg ctcggaatca ctccttttca atacaatccc
     1021 gttactaaag aacttatcgt ttataagaac atcgacgtag aaattcgttt tgaaggagga
     1081 aattctcaat ttggagaaaa tgcttatcgc aacccctatt ttgatgccat tttatccgac
     1141 aacctcctca attatagctc tttgtctcat atcgactatg ccgcccgtta tgctagctac
     1201 tatgctccca ctgaagatga tgaatgcgaa tatatcatca tttgccccga tggtcccgaa
     1261 tttcaaacct gggcagatag tattcgtaaa ttcagaaccg agcagggcat tcttactaaa
     1321 gtatttacac ttacccaaat tggtggaaat actaccaccg ctatcgaaac ttttatcaac
     1381 aatgcttaca acaactggag tataaaacca attgcctgtt tattgctagg tgattatggc
     1441 actaatgcta ccaatagtat catttcacct atttacaaca actattgcgt ttcagataac
     1501 atttatgccg atgttgatgg agacatgatg cctgatgtta tattttcgag gatcactgcc
     1561 cgtaatacta ccgaactaca agtcatggtt acaaaagtat tgaactatga acgaaatccg
     1621 ccaaccgaca ctacgtttta taaccgtcct atcactgcat tgggatggca gacagaacgt
     1681 tggttccaaa tttgttcgga ggtagtcgga ggctttttca aaaataaact tggcaaaaac
     1741 ccagtcaggc aaaatgcaat ctatcaggga aatcctaatg tcgacccgtg gtcaactgct
     1801 accaacacta acactgtact caattatttt gggcctaacg gacagggcta tatcccggct
     1861 acaccacagg agttaggagg ttggacgggt ggatctgcca caggtatcaa taacgccata
     1921 aacaatgggg cctttattat tcaacatagg gaccatggca tggaaactgg ttggggtgaa
     1981 ccttcctaca ccaacagcaa tatcaacgcc ctatccaaca ccaatcttac ttttgtgatg
     2041 accatcaact gcctaacggg aaaatacaac tggggcagtg agtgttttgc cgaaaaattc
     2101 cacagacata ccaagaatgg attgaacgcc ggtgcattag gcctcttggc cccttccgaa
     2161 acctcttata gcttcgttaa cgacacttat gtctgggggg tttacgacaa ctggtggact
     2221 gatttcatgc caagctatgc atctacacct gaacctcgtg gtgtacttcc ttcttttggg
     2281 aatgctgctg gcaaatattt cttgcgtcaa tcgagttggc ctt
//
LOCUS       DAORPD010000199         2321 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_8348_length_2321_cov_2.164607, whole genome shotgun sequence.
ACCESSION   DAORPD010000199 DAORPD010000000
VERSION     DAORPD010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8348_length_2321_cov_2.164607"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2321)
                     /locus_tag="PK976_03160"
     CDS             complement(<1..>2321)
                     /locus_tag="PK976_03160"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HRC66918.1"
                     /translation="QIVTNSGAPGAGATIRIRGGSSLSASNDPLFVIDGVPIDNTSIY
                     GTRDPLSTINPNDIETFTILKDASAAAIYGNRASNGVIIITTKKGTLAPGKKAPQFEY
                     NGGFSLYTIPKKREVFDGDQFRSIINERYANRPEVLALLGDANTDWQEEIYRSSFAHD
                     HTVSATGAIGALPYRASVGYANYDGILKTDNMQRTTFGLNLNPSLFDDNLKINLNGKG
                     MFIDQRFANEDAIGSAIQMDPTKPVHDSNAYGGYWTWLQANGNPVDQATDNPVALLEQ
                     KHDNADVSRFLGNAQFDYKMPFLPELRANLNLGLDYSKTNGTVYVPGNAAFAFDPQYG
                     GGTNTQYTQEKRNELLDFTLNYVKEVPTIFSKFDVMGGYSWQHFYREDTNSTTNDLNG
                     PFFIQKRIFDTSSNKSEFYLVSFFTRFNYSLFDRYLLTFTLRDDGSSRFSKDNRWGLF
                     PSVALAWRIMDEPWMKNMKTLSQLKLRLGWGITGQQNITNDDYPYLPRYTSSWATASY
                     ILGTDTLITLRPEGYDPNIKWEETTTTNIGLDYGFLNDRIYGSLDVYWRKTKDLINFI
                     PVPAGSNLSNYITTNIGDLENKGVEFAINGKPFVGPDFYWDLGVNATYNKNKITKLTA
                     SDDPNYLGVLVGTIAGGVGNTIQIHSVGYPSNSFFVYEQVYDANGKPIEGLYVDRNKD
                     GKINDDDRYHYKKPAADVYLGINSRMQYKNWDFIFSGRANIGNYVYDNFSSNNSTYER
                     LYRPEGPYLANVSADVAETGFKVPQYLSDYYIK"
BASE COUNT          666 a          468 c          507 g          680 t
ORIGIN      
        1 ttttgatgta ataatcggag agatattgag gaactttaaa gcctgtttcg gcaacatcgg
       61 cacttacatt ggccagataa ggtccctcgg ggcggtatag acgttcatag gtagaattat
      121 tagagctgaa gttatcatat acatagttgc ctatattagc ccgaccgcta aatatgaaat
      181 cccagttctt gtattgcata cggctgttga tgcccagata gacatcggct gcaggttttt
      241 tataatgata tctatcatca tcgttgattt ttccatcttt attgcggtca acataaagcc
      301 cttcgatggg ttttccattg gcatcgtaaa cctgttcata aacgaagaaa ctgttagaag
      361 ggtaacctac cgaatgaatt tggatggtat tgccaacgcc acctgcaatg gtacctacaa
      421 gtactcctaa atagttagga tcgtcacttg ctgttagttt ggtaatttta tttttgttat
      481 aggtagcatt gacacccaaa tcccagtaga aatctggacc aacaaaaggt ttcccattga
      541 tggcaaactc cactccttta ttttcgaggt cgcctatatt ggtggtgatg tagttggaaa
      601 gattagaacc tgccggcaca ggaatgaagt tgataagatc tttggttttt ctccaataaa
      661 catccagcga accatagatt ctatcgttaa ggaaaccata atcgagtccg atattggtag
      721 ttgtggtttc ttcccatttg atattcgggt cataaccttc gggacgcaag gtgatcaacg
      781 tatctgtccc cagaatgtaa gaagctgtag cccaagaact cgtgtaacga ggtaaatatg
      841 gatagtcatc attggtaatg ttttgttgac ctgtgatacc ccaacccaaa cgaagcttca
      901 attgggatag ggttttcata tttttcatcc aaggttcatc catgattctc caggctaagg
      961 caaccgaagg gaacaatccc cagcgattat ccttcgaaaa tctcgaagaa ccatcatcac
     1021 gcaacgtaaa ggtaagtagg tacctatcga acaaggaata attgaacctc gtaaagaaag
     1081 aaaccaaata aaattcactt ttatttgaac ttgtatcgaa aattctcttt tggataaaga
     1141 aaggtccatt caaatcattg gtcgttgaat tcgtatcctc acgatagaaa tgctgccatg
     1201 aataaccacc catgacatcg aatttgctaa atatggtggg aacttctttg acgtaattta
     1261 aagtaaaatc gagcagttca ttacgttttt cctgagtata ttgtgtgtta gtgccacctc
     1321 catattgagg gtcgaaagca aaagcggcat ttcctgggac ataaactgtt ccattggttt
     1381 tagaataatc gagaccaagg ttaaggttag ctctcaattc aggaaggaag ggcattttat
     1441 aatcgaactg ggcgtttcct aagaatcggc tcacatcagc attatcgtgt ttttgttcaa
     1501 gcagtgccac tggattatcg gtcgcttgat ccacaggatt tccattggct tgaagccagg
     1561 tccagtatcc accataagcg ttcgagtcgt gaactggttt ggtgggatcc atctgaatgg
     1621 ccgacccaat agcatcttca ttggcaaaac gctggtcgat gaacattccc tttccgttga
     1681 ggttaatttt taggttgtcg tcgaaaagag aaggattaag gttgagtcca aaggtcgttc
     1741 tttgcatatt gtcggtctta agaatgccat cataattggc atagcccacg gaagcacgat
     1801 agggcaatgc gccaatggca cctgttgcac ttaccgtatg atcgtgcgca aatgatgaac
     1861 ggtaaatttc ctcttgccaa tcggtattgg catctcccag caaagcaaga acttcaggtc
     1921 tgttggcata gcgttcattg atgatgctcc tgaattgatc accatcaaaa acttcacgtt
     1981 ttttagggat ggtgtagagg gaaaaacctc cgttatattc gaattgagga gcttttttgc
     2041 caggagctaa agtacctttc ttggtagtaa tgattataac tccattggat gcacgattac
     2101 catagatagc tgctgccgaa gcatccttca ggatggtaaa ggtttcgatg tcgttaggat
     2161 tgatggtact caatggatcg cgtgttccat aaatggaagt attgtcaata ggcacaccat
     2221 cgataacaaa aagaggatcg ttgcttgccg acaatgaaga acctccacgg atacggatag
     2281 tagctccagc acctggcgca ccactattgg taacaatctg c
//
LOCUS       DAORPD010000200         2311 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_8399_length_2311_cov_2.161791, whole genome shotgun sequence.
ACCESSION   DAORPD010000200 DAORPD010000000
VERSION     DAORPD010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8399_length_2311_cov_2.161791"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..581)
                     /locus_tag="PK976_03165"
     CDS             complement(96..581)
                     /locus_tag="PK976_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66919.1"
                     /translation="MKIEIKTEILPLRGLPLINFGDTIEDVESILGKPEETLMLEDIE
                     ERTLPAMVYLYWNSCLTFFFSTLEEKPFLIMIETDDLNAQLLDEKIFKMKPKQLIALL
                     SGQKLFDFEKEQERHEIRYTWDEWNIDFYFDGDKLSTVNWSAQLDDTGHVVIPEEPFK
                     W"
     gene            complement(659..1468)
                     /locus_tag="PK976_03170"
     CDS             complement(659..1468)
                     /locus_tag="PK976_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRC66920.1"
                     /translation="MELKVVVITGASSGIGRALAQEYGERGWHLALLARNFDKLLQLK
                     DELSGKASSILCLSTDVSKEEECKNAMDEIIATYGRIDVLINNAGISMRALFKDVDLN
                     VIRRLMDVNFWGTVYCTKYALSSLLANKGSVIGIVSIAAYVGLPGRTGYSASKFAIRG
                     FLETLRTENLRNGLHVLIAAPGFTATNIRSTALLADGSQQGQSPRNEGKMMTTTQVAK
                     KIVKAIDKRKKTLILTFWEGKFTVFLQKFFPSLVERLTYLHMAKEPDSPFK"
     gene            1510..>2311
                     /locus_tag="PK976_03175"
     CDS             1510..>2311
                     /locus_tag="PK976_03175"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC/Alx family metal homeostasis membrane
                     protein"
                     /protein_id="HRC66921.1"
                     /translation="MPAFLYFCNQFFTLLHSSVQISSIINVASVAPHHHWWIITAFSV
                     VIVIMLLIDLGVFNKKTHSIGTREALTWTIVWIVVSLLFGIVVYFESGLNSASDYIAA
                     YLIEKSLSMDNIFVFILIFSYFSVPSEYHHKVLFYGIIGAIIFRAIFIFAGVELIKLT
                     YLPPFEVNGTEVQLNVVLTIFGIFLVYAGLKTFNNHKEKQKDFSKNFVVRAAHRLFPF
                     SNEYDNGKFFTRQNGKRLATPLLLVVLVIESTDILFAVDSIPAIFAISN"
BASE COUNT          658 a          477 c          420 g          756 t
ORIGIN      
        1 gccgaactca cagctcgagc tatcgatgaa tatgtgaacg aacaaaagaa atcagtcaga
       61 gaagctcgtt tgatagaatc ttaaatcttt ggcatctacc atttgaaagg ttcttctgga
      121 ataactacat ggccagtatc atctaattgt gccgaccagt ttactgttga cagtttatcc
      181 ccatcaaaat aaaagtcgat gttccattca tcccaggtat agcggatttc atgtctttcc
      241 tgttctttct caaaatcgaa aagtttctgg cccgataata gggcaatcaa ctgcttgggt
      301 ttcattttga aaattttctc atcgagcaat tgcgcattca aatcatctgt ttcaatcatt
      361 attaaaaagg gtttctcctc taaggtgctg aaaaagaaag tcaaacaact attccaatat
      421 aaatagacca ttgcagggag tgtcctttct tcaatgtctt cgagcatcaa agtttcttcg
      481 ggttttccaa gaatagattc cacatcttct attgtatcgc caaaattaat caggggcaat
      541 cccctcaagg gcaggatttc cgtttttatt tctattttca tattgggata aaattttata
      601 ccattcacga agttcaatta ttaatacacc ttaaaagaac tgaaaaaatc atgataagtt
      661 atttaaaagg cgaatcgggt tccttggcca tgtgcaaata agttaaacgc tcgaccagtg
      721 aaggaaagaa tttctgaaga aaaaccgtaa attttccttc ccagaaggta agaatcaatg
      781 ttttctttcg tttgtcaatg gctttgacta tttttttggc tacctgagtg gtagtcatca
      841 tctttccctc gttgcgtgga gattgtccct gttggcttcc atcggccagc agagcggtgg
      901 agcgtatatt ggtggcagta aaaccaggtg cggctatcag tacatgaagt ccattacgaa
      961 gattttctgt ccggagggtt tctaaaaatc cccgaatggc aaatttcgaa gccgaataac
     1021 ccgttcggcc gggcaagccc acataagccg caatggatac tatgcctatc acgctcccct
     1081 tgtttgctaa aagtgaagac aaggcatatt tagtgcagta aaccgttccc cagaaattaa
     1141 catccatcaa tcgccgaatc acattcaaat cgacatcttt gaataaagca cgcatggaga
     1201 tgccagcatt gttaatcaaa acatctattc ttccataagt cgcaataatt tcatccatgg
     1261 cattcttgca ctcttcttcc ttacttacgt cggttgataa acaaagaatg ctggaagctt
     1321 ttccggaaag ttcatcttta agttggagta atttatcgaa gttgcgtgct aataaagcca
     1381 aatgccatcc tctttctcca tactcctgag ctaaggccct gccaatgccc gacgaagctc
     1441 cagtaatcac tacaactttc aattccattt ttttcgaaaa taaatatgcg aaaataatga
     1501 attagtgttt tgcctgcatt tctttacttt tgcaaccaat tttttaccct tcttcattcg
     1561 tccgtgcaaa tttcttctat aataaatgtt gcctctgttg ctcctcatca tcattggtgg
     1621 ataattacgg ctttcagtgt cgtaattgtc atcatgctcc tcattgattt gggagtcttc
     1681 aataaaaaaa ctcattccat tggtacccgt gaagcactta catggacaat tgtctggatt
     1741 gtggtttcgc tgctttttgg gatagtggtg tatttcgaat cagggctcaa cagtgcgagc
     1801 gattacattg ccgcctacct catcgaaaag tcgctttcga tggataatat tttcgttttt
     1861 atcttaatat tcagttattt tagtgttcca tcagaatatc accacaaggt acttttttat
     1921 ggaatcattg gtgccatcat atttagagcc atatttatct ttgctggcgt tgaattgatc
     1981 aagcttacct atctgcctcc ctttgaagta aatggaactg aggttcaact caatgtcgta
     2041 cttacgattt ttggaatttt tctggtttat gcaggattaa aaaccttcaa taatcataag
     2101 gaaaaacaaa aagatttttc gaagaatttc gttgttaggg cagcccatcg tttattccct
     2161 ttttcgaatg aatacgacaa cgggaaattt tttaccagac aaaatgggaa acgacttgct
     2221 acgcctttat tattagtcgt tttggttatc gagagtaccg atattttatt tgccgtagat
     2281 tcaatccctg ccatttttgc tattagtaac g
//
LOCUS       DAORPD010000201         2297 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_8475_length_2297_cov_2.830955, whole genome shotgun sequence.
ACCESSION   DAORPD010000201 DAORPD010000000
VERSION     DAORPD010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8475_length_2297_cov_2.830955"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="PK976_03180"
     CDS             complement(<1..461)
                     /locus_tag="PK976_03180"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HRC66922.1"
                     /translation="MILLCLTCFFVGYLIGSILFAHLITRIISGKNIRELGNLNPGAY
                     NVFTQVNKFWGVVSGLLDAVKALLPMMIASYFFDVPNIALGCLGIGAIFGHRYPLYYH
                     FKGGRGASTLLGMFLFFIPIEFGISLVVDAILVLGFIRKQYGTWGPSILIAL"
     gene            complement(479..1363)
                     /locus_tag="PK976_03185"
     CDS             complement(479..1363)
                     /locus_tag="PK976_03185"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HRC66923.1"
                     /translation="MEKKIFFITADNLGLTREQIDYPHLEILKFPVIIDGIGYRQSDE
                     YNAKWLISKYENENIVAKSSTLVQKEIVDIVEKYHNDFDMMIHVLMSSGMSAASFKVA
                     ENVRKQFENVIPIINIDSRQAINGVGNVLLAIIDIMKANPILSLEEIDQKAQEVVEST
                     FSYFVLPDLKYLYKGGRVGKAQSLMGSILHIIPVVGLIGTEKEGIIVPIGKGRTFKQV
                     NSLIYDKITEKMNEKGVSKIKRIISIFGYGDKNADAFTDLKEKVQAIPCDDFIEGKPA
                     IVDAVYCGPGAYGVSVYL"
     gene            complement(1374..>2297)
                     /locus_tag="PK976_03190"
     CDS             complement(1374..>2297)
                     /locus_tag="PK976_03190"
                     /inference="COORDINATES: protein motif:HMM:NF025064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66924.1"
                     /translation="SWNPEIKYRYCTEFTLESDHISKEHLIELLNGYGDSLIIIQSNN
                     RFKVHIHTNKPNDVFEQASEYGNLLFTKVDDMKKQHRNFISEDIIGFERDKSVFCIVS
                     GRGFAEILKNLGADDILCYGKNKPSVDQLLKNLNNLKAKNIIAAADDHDILMALKLAI
                     SLCKSNVYVVESDNPISLISIMMNMPKDYDITTMFEEAMKNLHNIPFSSIARATRDVS
                     GINGIEVKKKDYFAIYNKSIITSNPRLNSLIEEIIGRFAADSVLITFYRGEKVKNNAE
                     LLQVLKIKYQTIEFEEYYGGQSKYDYYITFE"
BASE COUNT          701 a          478 c          370 g          748 t
ORIGIN      
        1 agcgctataa gtatagaagg cccccatgtg ccatattgtt ttcttataaa acctaaaacc
       61 agtatggcat ctacgaccaa agaaatacca aactcgatgg gaataaaaaa tagaaacatc
      121 cctagtaaag ttgacgctcc acgcccacct ttgaaatgat aataaagagg gtaacgatgt
      181 ccgaatatgg cacctattcc taagcaacca agagcgatgt tgggaacatc gaaaaaataa
      241 ctggcaatca tcatgggcaa cagtgcctta acagcatcca ataatcctga caccacaccc
      301 cagaatttat tcacctgggt aaaaacatta taagcacctg gattgagatt tcccagttct
      361 ctgatgtttt ttccagaaat aattcttgta atcaaatggg caaaaagaat ggagcctatc
      421 aggtatccaa caaaaaagca agtcaggcat aatagaatca tattcataac aaaaaagttt
      481 acaaatagac tgaaactcca taagcgcctg gtccacagta aacagcatct acaatcgccg
      541 gtttcccctc tataaaatca tcgcagggaa ttgcttgcac cttctctttt aaatcggtaa
      601 aggcatcggc atttttatcg ccataaccaa aaatcgaaat gattcttttt attttactta
      661 cgcccttttc gttcatcttt tcggttattt tatcataaat caaagaattg acttgcttaa
      721 aggttcttcc tttgccaata ggcacaatta tgccttcttt ttcagttcct attagcccaa
      781 caacgggtat aatatgcaaa atacttccca tcaatgattg ggccttccct acccttcctc
      841 ctttatacaa atatttcaga tcgggtaaaa caaaataact gaaggtgctt tccactactt
      901 cctgggcctt ttgatcgatt tcttccagac tcaaaatggg attggctttc atgatgtcga
      961 tgattgctaa cagaacattc cccacaccat tgatggcttg ccggctgtca atatttatta
     1021 ttgggatcac gttttcgaac tgtttacgaa cgttttcggc cactttaaac gaagccgccg
     1081 acattccgct actcatcagc acatgaatca tcatatcgaa gtcattatgg tatttctcca
     1141 caatgtctac aatttctttt tgaactaaag tggatgattt agctacgata ttctcatttt
     1201 catattttga aatcaaccat ttagcattat actcatcgct ctggcgatat cctatcccat
     1261 cgatgataac cggaaatttc agaatttcaa gatgaggata atcgatttgt tctctggtaa
     1321 gccctaaatt atcggctgta ataaaaaata ttttcttttc cataacggtg attttattcg
     1381 aaagttatat aataatcata tttcgactgg cctccataat attcttcgaa ttcgatagtt
     1441 tgatatttga tttttaaaac ttgcagtaat tcggcattgt ttttaacttt ttcccctcta
     1501 taaaaggtaa ttagaacact atcagcggca aatctgccaa ttatttcctc gatgagtgag
     1561 tttaatcgag ggttggaggt tattattgat ttattgtata ttgcaaaata atcctttttc
     1621 ttgacttcta tgccatttat gccagataca tctcttgtgg ctcgagctat ggaagaaaag
     1681 gggatgttgt gaaggttttt cattgcttct tcgaacatgg tagtaatatc gtaatctttc
     1741 ggcatattca tcattatgct gatcaaagaa atgggattat ccgattcgac cacatacaca
     1801 ttcgatttgc ataacgaaat agctaatttc aaagccatca ggatatcatg atcgtcggcc
     1861 gcagcaatta tattctttgc tttcaggtta ttcagattct tcaaaagctg atccaccgag
     1921 ggtttattct tgccataaca gagaatatcg tcggctccca aattttttaa tatctcagca
     1981 aatcctcttc ccgaaacaat acagaaaaca cttttatcgc gttcgaatcc aataatatcc
     2041 tcactaatga aatttctgtg ctgtttcttc atatcatcta cttttgtaaa cagcaagttg
     2101 ccatactccg atgcttgttc gaaaacatca ttgggtttat tggtatgaat atggaccttg
     2161 aatctattgt tgctctgaat tattatcaaa ctatcaccat aaccgttcaa taattctatg
     2221 agatgctctt tcgaaatgtg gtcggattcc aaggtaaatt ctgtgcaata tctatatttg
     2281 atttctggat tccacga
//
LOCUS       DAORPD010000202         2295 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_8484_length_2295_cov_2.174554, whole genome shotgun sequence.
ACCESSION   DAORPD010000202 DAORPD010000000
VERSION     DAORPD010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8484_length_2295_cov_2.174554"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(272..841)
                     /locus_tag="PK976_03195"
     CDS             complement(272..841)
                     /locus_tag="PK976_03195"
                     /inference="COORDINATES: protein motif:HMM:NF015674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HRC66925.1"
                     /translation="MKNRSFTSSRSALIRGLIAILIGGLAVFIPGLTLEGIIFSAGLC
                     VLIGGLVSLIFALREKNLGAKNILLLQTVFNLIIGILFIAMPQNIVKFFVIILGIGLL
                     FLGAFQLMVGLSVRSRYGWSWFYFSISLIVIVAGIIMLYDPFKSAQALLIFLGVLLML
                     YGISELVMAWHLRKELKTLTEEGDIIEPE"
     gene            complement(1418..>2295)
                     /locus_tag="PK976_03200"
     CDS             complement(1418..>2295)
                     /locus_tag="PK976_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRC66926.1"
                     /translation="LLRVLETGEFIRVGSSKVLKTDVRVVAATNIDVHAAIEKGKFRQ
                     DLYYRLNSVPIIIPPLRERKEDIYLLFRKFALDFAEKYRMPPIQLDDEAQQMLINYRW
                     PGNIRQLKNITEQISIIEQDRLVTAQILAKYLPDPHHHDLPVLYHKEFLSSQEISERD
                     LLYKVLFEMKKDINDLKKLVFEIINQNGITQEMKQNNAPIINKLVRDYKDDPGFIAKS
                     SPFEDVHYEVAGKIDEPIIEPEVIDDSLSLQKKEIELIKRALAKHNGKRKGAAAELGI
                     SERTLYRKIKEFNLE"
BASE COUNT          755 a          465 c          401 g          674 t
ORIGIN      
        1 ggcaaccata gtcccgacag acaccgtacc cattatccgt aaccatgaca ctgtgatgta
       61 ttttcttata tatcacctga aaaatttaca aactctgcct gctgatcccc ttacaaccat
      121 aggaaattag tacacatcct atgaagggag atcctctctc ccccgttatc aaccgtaaaa
      181 tgtatggcca aatgcttctg acatgaaaaa caggtaagcc atgcataaag cagacgtaaa
      241 gggaaaagtc tatactgtcg acaagatttt cttattcagg ttcgataata tcgccttctt
      301 cagtgagggt tttcaattct ttacgcaagt gccacgccat aaccaactca gaaattccat
      361 agagcataag caatacaccc agaaaaatga gcaaggcctg agcactctta aacggatcat
      421 atagcatgat gatgcccgca acaataacta tgagagaaat ggaaaaataa aaccacgacc
      481 acccatatcg gctcctaacg ctcaatccaa ccattagctg aaatgcaccc aaaaacaaaa
      541 gaccaatgcc caatatgatg acaaagaact tcactatatt ttgaggcata gcaataaata
      601 atatccctat aatcaaattg aacaccgttt gaagaagcag aatattttta gctcccagat
      661 ttttttctct aagagcaaaa attaaagaga caagacctcc tattaaaaca cataaaccag
      721 cagaaaagat aatgccttcg agagtcagtc ctggaataaa cacagccaat cctcctatca
      781 atatggcaat taggcctctt ataagagcac tgcgcgaact cgtaaaactt ctgtttttca
      841 tagaatatgt tttttaagtt taaagcgaaa ttatacaaaa acctataata aacaagatcg
      901 ataaaccaaa taatgattta tcagtatata tgagctataa aattagcgac ttcgatgagt
      961 atccatttat tcaataacca gacttcaatg aattgattta ttttgaatta atcacaaatt
     1021 tcagctctct atcatagtaa tcgacctcaa tatttccgac gatattcatg caaatgttgg
     1081 aatgaaaata agaatccttt tgctgattca tgattaccat aaagcaaact ggtggttgca
     1141 ccaatgggta ctcttgcctg cacttgtgaa tcgattgaaa agccatttga gtttggtttg
     1201 gataattggg gaaaaatata tctgaacgaa ctgcaatcat atctatgacg aaacctaata
     1261 aaatattcga tttcaggatt tggccaataa gcgcaaaagc cctgtatgtg gttcgttcaa
     1321 gtcaaacttc gaagagttga aataaacacc tcagcatgac ctggtctatt gataaaaatc
     1381 gagagaatga aaaatcaata agtttttatc tcatcaatca ttcaagatta aattccttga
     1441 ttttacgata gagggtgcgt tcagagatgc cgagttcggc tgcggctcct ttacgtttac
     1501 cattgtgttt ggccaatgcc cgcttgatga gttcgatttc cttcttttga agtgaaaggc
     1561 tatcatcaat tacttcgggt tctataatag gttcatcaat tttgccggct acttcgtaat
     1621 gcacatcttc aaaaggcgaa gatttggcga tgaaacccgg atcatcctta taatctctta
     1681 ccaatttatt aattatagga gcattattct gtttcatctc ctgagtaatc ccattctggt
     1741 taataatttc aaatacgagt ttcttcaaat cattgatatc ttttttcatt tcgaaaagga
     1801 ccttataaag caagtcgcgt tctgaaattt cctgactcga aagaaattcc ttgtgataga
     1861 ggactggcaa atcgtgatgg tgggggtcgg gaagatattt agctaatatt tgggcagtca
     1921 ctaagcgatc ttgttcgatg atactgattt gttcggtaat atttttcaat tgacgaatgt
     1981 tgccaggcca gcgataattg ataagcattt gctgggcttc atcatcgagt tgaattggag
     2041 gcatacgata cttttcggca aaatcgaggg caaattttct aaacaacagg taaatatctt
     2101 ctttccgctc gcgtaaaggc ggaataataa tagggacaga attcaaacga taatacaaat
     2161 cttgccggaa tttacctttt tcgatggcag cgtgtacatc tatattggta gctgctacta
     2221 ccctcacatc tgttttcaat actttcgatg agccaaccct gatgaattcg cctgtttcaa
     2281 gcaccctcag caatc
//
LOCUS       DAORPD010000203         2291 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_8505_length_2291_cov_2.097943, whole genome shotgun sequence.
ACCESSION   DAORPD010000203 DAORPD010000000
VERSION     DAORPD010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8505_length_2291_cov_2.097943"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            849..1358
                     /locus_tag="PK976_03205"
     CDS             849..1358
                     /locus_tag="PK976_03205"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HRC66927.1"
                     /translation="MKKIFLFTGIVFISLSFVSSGKNQKTESTESKSKETTSPVQIIK
                     LDNGLFCKTIWDYTNSPDRWKYRGNKPSVIDFYADWCKPCKIASLILEDLAREYAGEV
                     NFYKIDTDREKELATLFGIRTIPSFLFIPKEGEPRMTAGIAPGEEATRKMFNDYIQQY
                     CLGNFSEAQ"
     gene            1408..1887
                     /gene="trxA"
                     /locus_tag="PK976_03210"
     CDS             1408..1887
                     /gene="trxA"
                     /locus_tag="PK976_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRC66928.1"
                     /translation="MKKINLKLILTIFVFTFSILDFFGQASTKTISNEGKVIYLTSAE
                     FKEKVFNWEKSPENWVFEGDRPCIVDFYADWCKPCRMVAPIMEELALKYKGKLTIYKV
                     NTDKEKEVSSVFGIRSIPSVLFCPTKGKPQMATGALPKDTYIQVIEEFLLGNKKQTR"
     gene            1946..>2291
                     /gene="trxA"
                     /locus_tag="PK976_03215"
     CDS             1946..>2291
                     /gene="trxA"
                     /locus_tag="PK976_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005643748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRC66929.1"
                     /translation="MENLTKQTFLEKVFDYEKNDQWKFEGKLPCIIDFYADWCGPCKV
                     VAPILEELSKEYAGRVNFYKVDTEAEQELAAAFGIRSIPSILFCPVDGQPQMAVGVLP
                     KDSFKQAIEDVLL"
BASE COUNT          771 a          409 c          422 g          689 t
ORIGIN      
        1 ctgccactaa aaattaattt agcattcaat aatctatttt gataattaat atattaatgt
       61 cctaaaatag aattcattat gcaaatccaa aattaatata gttttcgaat aaataaaata
      121 aagtaaaata tttttcaaga taaggatgaa aaaatcattc aattatgttg tgcaatcaat
      181 ttatacagtc attttcgacg atggaggttt tgatgaagcg gtttgaaaac ttattaaaaa
      241 ttctttaaac ctgattccag gcattccaca attttcttct tcagtttcca gcaagtcatt
      301 tgtcgtagat taaaatatct ggccaattca tagctcgaaa ttttgggatt gtggcaaacg
      361 atattggcaa atcgaaaggc ttccggcaaa ggtaaacggc aatagtgaaa aattgtatgg
      421 gccttggccg attcttcacg tttacatcgc gtacaccgcc tcgaatatgg tgtattcccc
      481 ttacaataat tgttatgcca gcaattgcga caaatatatc cgccttccca ttttttcaag
      541 gctaagtatt ccaagcaaga ttcctcactg gcaaattttt tgcagatgcc attaaaatca
      601 gtgtgatgaa atatttcaac aattccatcc atgacaacat gtcatattta aagttccaaa
      661 tgtaaaattt tatctactcg cagtttattc aataaaaact ctattcgttc gaacataaat
      721 gcatttaatt tgtttttggg atgttagtct catatcggtc taatctttgc aaagataaaa
      781 aagtgataaa aatgcgattt gattacaaga attcgtttaa ttttgctacc tcaaaaccaa
      841 gtgtcgatat gaagaaaatt tttctcttta cgggaattgt ttttatctct ctgtcattcg
      901 tttcaagtgg aaagaatcaa aagactgaat cgactgaaag caaatcgaag gaaacaactt
      961 ctcccgtaca gataatcaag ctcgacaatg gacttttttg caagacaata tgggattaca
     1021 cgaattcacc cgatcgatgg aaatatagag gtaacaagcc gagtgtcata gatttttatg
     1081 ctgattggtg caaaccctgt aaaatcgcct ctctcattct cgaagattta gccagggaat
     1141 atgcagggga agtgaatttt tacaaaattg atactgatag ggaaaaagaa ttagctactc
     1201 tatttgggat aaggaccata ccttcgtttc tttttattcc caaagaaggc gagccccgga
     1261 tgacagccgg tattgcccca ggggaagaag ctacgcgtaa aatgttcaac gattatattc
     1321 agcaatactg tctgggaaat tttagcgaag cacaatagtt taccaattga atacaaattt
     1381 gatataccaa tccaaaggcc tggaataatg aaaaaaataa atttaaaact catactcacc
     1441 atttttgtgt tcaccttctc gattctggat ttttttggtc aagcatcaac gaaaaccatt
     1501 tcaaacgaag ggaaagtaat ttatctaact tcagccgagt ttaaagaaaa ggtatttaat
     1561 tgggaaaaaa gccctgaaaa ctgggtattt gaaggcgatc ggccttgtat tgtagatttc
     1621 tatgccgatt ggtgcaagcc ttgtagaatg gttgctccta ttatggaaga gttagcctta
     1681 aaatataaag gtaaactaac catttacaaa gtgaatacgg ataaagaaaa agaggtctcg
     1741 agcgtattcg gtattcgcag cattccctcg gttcttttct gccccacgaa agggaagcca
     1801 cagatggcta cgggtgcttt acccaaagac acttacattc aggttatcga ggagtttctt
     1861 ctgggaaata aaaaacagac ccgataacaa gaatctaaat aattacaatt cattacggta
     1921 aaatacataa actcatttct attctatgga aaatttaacg aaacagacct ttctcgaaaa
     1981 agttttcgat tatgaaaaaa acgatcaatg gaaattcgag ggcaaattgc catgcatcat
     2041 tgatttttat gcagactggt gcggcccttg taaggtggta gcacctattc tggaagaatt
     2101 atcgaaagaa tatgctggca gggtgaattt ttacaaggta gataccgaag ccgagcagga
     2161 actggccgca gcttttggca tcagaagcat accctctatt ctgttctgcc ccgttgatgg
     2221 tcaaccacaa atggcggtgg gtgtattacc aaaagatagc ttcaaacaag ccatcgaaga
     2281 tgtactttta a
//
LOCUS       DAORPD010000204         2290 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_8511_length_2290_cov_2.300671, whole genome shotgun sequence.
ACCESSION   DAORPD010000204 DAORPD010000000
VERSION     DAORPD010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8511_length_2290_cov_2.300671"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="PK976_03220"
     CDS             <1..370
                     /locus_tag="PK976_03220"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRC66930.1"
                     /translation="ENGTYWVKVSNRGCYKIDTIQVQMCEGYVWAPTAFSPNADGLNE
                     TFKIITSDETINFQMYIYSRSGMLVYKTDDITKGWDGKDMNGEICPSGVYVWKIVYKG
                     NSPTAPGIEYTRSGVVTLIR"
     gene            492..1379
                     /locus_tag="PK976_03225"
     CDS             492..1379
                     /locus_tag="PK976_03225"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRC66931.1"
                     /translation="MASTKTHKVHYSTISSISSIKKYIFKGSPIKLVGILLFFVSSTV
                     MGGDTLSFTAKTYFKSYFTDAFPIITLPKEMTDADWAFAGFAATSGGWLMAYDKEAYD
                     FIRDKRGDFTQNISRYFLEPLGSGLITLPALLAMYGYNQWQGQSYHSEAALQAIKAFI
                     ISATFAEGAKLLLHRWRPSKTDDPYRFDGPSLSFERDAMPSEHTSTAFAVATVLAEYY
                     HDNWLITAVSYSMASLVGLSRIHDGKHWPSDVFAGALLGYTVGKTVSKHGNSLKNKKI
                     IVHPLVNSYETRICIIYRL"
     gene            complement(1737..>2290)
                     /locus_tag="PK976_03230"
     CDS             complement(1737..>2290)
                     /locus_tag="PK976_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66932.1"
                     /translation="IKYSVKSKKIIMKQVFLAHTQEKVAIIVLAAVLFSMLSCNKSSK
                     QVAIARLNSKDSSFEFSMDTNRLKALMIEGFDIDRSDSNILLTPTAIYYANIMVSDTH
                     TAPVMAYFKERLPKGSQATITFTTTENKSDIGSIFKTRVILAADAEPIHFDGLTKMEA
                     KTQINGIKISKIRVLIGLKKGNL"
BASE COUNT          671 a          461 c          449 g          709 t
ORIGIN      
        1 agaaaatggc acttattggg ttaaagtatc caacagagga tgttataaaa tagataccat
       61 tcaggttcag atgtgtgaag gttatgtttg ggctcctaca gccttctcac caaacgccga
      121 tggccttaat gaaaccttca agataattac ttctgacgaa acgatcaatt tccagatgta
      181 tatctattca cgctcgggta tgttggttta taaaaccgat gatatcacca aaggctggga
      241 cgggaaagat atgaatggtg aaatctgccc ctctggagtt tatgtgtgga aaattgttta
      301 taaagggaat agcccaacgg ctccaggtat tgaatatacc cgctcgggag tcgtaacact
      361 gataagataa taacttaacc tcattgcttg cataaaggct gtcattgctc taatttgctt
      421 tgacagcctt ttttctctca agggattcaa ttcgtttggc ctttttccct attattgtcg
      481 aaaaattcac gatggcatcg acaaaaacac ataaagtcca ttactctact atttcttcca
      541 tctcttctat aaaaaaatat attttcaaag gatcacccat caaattagtg ggtattcttc
      601 tgttttttgt ttcttcgacg gtaatgggtg gagatacatt atctttcacg gcaaagacct
      661 atttcaagag ttatttcacc gatgcctttc ccatcattac tttgcccaaa gaaatgaccg
      721 atgccgattg ggcttttgca ggatttgccg ctacgtctgg cggttggctc atggcatacg
      781 ataaggaagc ttatgatttt atcagagata aaagaggaga ttttacccag aatatctccc
      841 gctatttcct cgaaccactg ggttcaggat tgattacatt gccagcacta ttagctatgt
      901 atgggtataa ccaatggcaa ggacagtcat atcattcaga agcagccttg caagccatta
      961 aagcctttat tatttctgca actttcgccg aaggtgcaaa attattactt caccgatgga
     1021 gaccttccaa aacagatgat ccctatcgtt tcgatgggcc aagtttatca ttcgaaaggg
     1081 atgcaatgcc ttcggaacac accagcactg ccttcgcagt ggcaactgtc ttagccgagt
     1141 attaccatga taactggctc atcacagcag tttcttattc aatggcaagc ttggtgggac
     1201 tatcgagaat ccatgacggg aaacattggc cttcagacgt atttgcagga gccttactgg
     1261 gttatacagt gggtaaaact gtttccaaac atggtaattc gctaaagaac aagaaaatta
     1321 tcgtgcatcc attagtaaat tcctacgaaa cgagaatttg catcatctat cgattgtaag
     1381 ttttctgaaa atagttggcc atactataac ctgggttaag gctggctgtt ttgaacaaac
     1441 tgtttcaaac cctactgcca aggatttact ttcttaataa ttttccaaga ctataattgg
     1501 tatttaggcc ggctgcgtat gcatatccga tatctttgct gcatccttca tttttcacag
     1561 gtggatgccc ttcgcaataa caaatcaaat cctctgaaaa aagaggtttg caggagagcg
     1621 tggtatctct ttatatacaa gaaatgcagg ttttgtgttt cggattctgt tcttcaatga
     1681 gaaataaatt agtaccagat ttctttaaac gaaagaaaag tgccaccgac agttggtcat
     1741 aaattccctt tcttcaagcc aataagcacc ctaattttcg aaattttgat gccattaatt
     1801 tgggtttttg cctccatctt cgttaagcca tcgaaatgta tgggttcggc atcagcagct
     1861 aaaatgactc tggtcttgaa tatactgcct atatcgctct tattttcggt agttgtaaaa
     1921 gtaatggttg cctgagagcc cttgggaagt ctttccttga aataagccat gacaggagct
     1981 gtatgcgtat cacttaccat aatattggca taatagatgg cggtgggggt taatagaata
     2041 ttagaatcgg agcgatcaat atcgaaacct tcaatcatca aggctttcag ccgattggta
     2101 tccatcgaaa actcgaaact gctatccttc gaattcaatc tcgcaatagc aacctgcttg
     2161 ctacttttgt tacacgaaag catagaaaat aggactgctg ccaggacgat aatcgcaact
     2221 ttctcctgag tatgtgctaa aaaaacttgt ttcataatga ttttttttga tttaacagaa
     2281 tatttaataa
//
LOCUS       DAORPD010000205         2281 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_8555_length_2281_cov_2.280323, whole genome shotgun sequence.
ACCESSION   DAORPD010000205 DAORPD010000000
VERSION     DAORPD010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8555_length_2281_cov_2.280323"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="PK976_03235"
     CDS             complement(<1..404)
                     /locus_tag="PK976_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase"
                     /protein_id="HRC66933.1"
                     /translation="MNKIALQAVVELFERYKGAKPSSVHHLPPSASERKYFRLNVEND
                     SILGAWNPCIQENEAFFSFTYQFLNKDLPVPPIYAISDDRKAYLLRDLGNVTLFSHLQ
                     ELRKSMSWNEQLTETYRQTLDALIRFQLAGKG"
     gene            complement(594..664)
                     /locus_tag="PK976_03240"
     tRNA            complement(594..664)
                     /locus_tag="PK976_03240"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(630..632),aa:Gln,seq:ttg)
     gene            855..>2281
                     /locus_tag="PK976_03245"
     CDS             855..>2281
                     /locus_tag="PK976_03245"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HRC66934.1"
                     /translation="MKNYKRINNLLGWITFAIAFAVYMLTLEPTASWWDCGEYISTAY
                     KLQVGHPPGAPTFQLLGRLFTLVAGNDVTRVAYMINTMSALSSALTILFLFWSITMLA
                     RKVVLKQGQEINTAKLIAIMGSGFIGAMAYTFSDSFWFSAVEGEVYAMSSFFTALVFW
                     AILKWENVADEDHAVRWLIFIAFMIGLSIGVHLLNLLAIPAITYIYYYKKYKPNWKGF
                     LLTGVISIALLALIMYGIIPEIVNLFGKTELFFVNSLGLPFNSGTLFFALVLALMIVS
                     GLLYTIKGDKYKNFFLITLIIFFLILIGSGKGFLGVVISLAVAAISIYVVLKLKKARP
                     VINAVILSLAFILIGYSSFLMLVIRANANTPINENAPTDAIGLLSYLNREQYGSWPIF
                     YGQYYNTPVVDFKDQPPIYQKDEKSGKYVIADPRKGTLPVYDKRFMTLFPRMWSNQKQ
                     SHINAYKNWGKIKGVPITITNKSGKT"
BASE COUNT          720 a          452 c          428 g          681 t
ORIGIN      
        1 aaacctttac ctgcaagctg aaatcgaatg agagcatcta aggtttgccg gtaagtttcg
       61 gtaagttgtt cattccagct catggatttt cgtagttcct gcaaatgact gaaaagagta
      121 acattaccca aatccctcaa gaggtaggct ttgcggtcat cagagatagc atagatagga
      181 ggaacaggga ggtctttatt gaggaactga taggtaaaag aaaaaaaagc ctcattttcc
      241 tgaatacatg gattccaagc tccaagaatg gaatcattct caacgttcaa tcgaaaatat
      301 ttacgttcgg aagccgaagg aggcaaatga tgtacggaag atggtttcgc acccttatat
      361 ctttcgaaaa gctctacaac agcctgtaat gcaatcttat tcaatgaaaa aattcaattt
      421 atatgacaaa agtaatcagc aaaattcgaa tgagatatcg ttaaagagga atgaaatagc
      481 aaatcaatct ataaaataat gaataacaaa tagttaataa gaatagcaaa aatcctctct
      541 caaagcttag gatagacata ttaaaaaatg agccccgcat tcaccggggc ttgttgtccg
      601 accaggattc gaacctagac taagagaacc aaaatctcct gtgctgccat tacaccatcg
      661 gacaaaattt tcggcggcaa agataaaaaa aatcctctgt tcaagttgta tgccacataa
      721 ttttacaagc cataaatttt ttattacttt cgcactcgat tcataatatt aaaatttcaa
      781 agtgtttaat ggtttaatcg atagacaatc agtcggaaaa aaatactgat actttgaatt
      841 taatcagtaa aaacatgaaa aattacaaac gaatcaacaa cctgcttgga tggatcacct
      901 ttgccattgc ttttgcagtt tacatgctga ctctcgaacc cacagccagc tggtgggatt
      961 gtggagaata catttcaaca gcatacaaat tacaggttgg gcatccacca ggagcaccca
     1021 ctttccaatt attaggccgt ttatttaccc ttgtagcagg caatgatgtt acgcgagtag
     1081 cttatatgat caacaccatg tcggctttaa gcagtgctct gacaatcctg ttcctttttt
     1141 ggagcattac gatgcttgcc cgaaaggtgg ttttaaagca aggtcaggag atcaatacag
     1201 caaaattaat tgccatcatg ggctcgggat tcattggagc tatggcctat actttcagcg
     1261 actcattctg gttctcggct gtcgaaggag aagtttatgc catgtcgtct ttcttcacgg
     1321 ctcttgtctt ctgggcaatc ttaaaatggg aaaatgtggc tgacgaagat catgccgttc
     1381 gctggcttat ctttatagca ttcatgattg gtctttccat tggagtacat ctactgaacc
     1441 tcttggctat tcctgccata acttatatct attattacaa gaaatacaag cccaactgga
     1501 aaggattcct gcttacagga gtcatttcta tagccttact ggcactgatc atgtatggaa
     1561 tcattcccga aatcgttaac cttttcggca aaaccgaatt gttcttcgtt aattcacttg
     1621 gattgccatt caactcggga actcttttct ttgcattagt gcttgcctta atgatagttt
     1681 caggcttact ttatacgatc aaaggcgaca agtataaaaa tttcttccta attaccctca
     1741 ttatcttttt tttgatttta atcggatcgg gaaagggatt tttaggcgtt gtgatctcat
     1801 tggctgttgc agcaattagt atctatgtag ttcttaaatt gaagaaagca cggcctgtta
     1861 ttaatgcggt cattttatcg ttagcattta ttcttatagg ttattcttca ttcttaatgc
     1921 tggttatacg tgccaatgcc aacacaccta ttaacgaaaa tgctccaacc gatgccattg
     1981 gccttctttc gtatctaaat agagaacaat atggctcatg gcctatattt tatggccagt
     2041 actacaatac acccgttgta gattttaaag accaaccacc tatttatcaa aaagacgaga
     2101 aatcgggaaa atatgtgatt gccgatcctc gaaaaggtac tctgcctgtg tacgataaac
     2161 gattcatgac cttgtttccg cgaatgtgga gcaatcaaaa acaaagtcat atcaatgcct
     2221 ataaaaattg gggcaagatt aaaggagttc ccattacaat aaccaacaaa agcggtaaaa
     2281 c
//
LOCUS       DAORPD010000206         2281 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_8556_length_2281_cov_2.052111, whole genome shotgun sequence.
ACCESSION   DAORPD010000206 DAORPD010000000
VERSION     DAORPD010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8556_length_2281_cov_2.052111"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /gene="nth"
                     /locus_tag="PK976_03250"
     CDS             <1..382
                     /gene="nth"
                     /locus_tag="PK976_03250"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HRC66935.1"
                     /translation="ATLLEKFSGQVPDNLDDLESLPGVGRKTANVILSVAFNKPAMPV
                     DTHVHRVARRIGLVKDARTPLETEKQLTRYIPAHYISKAHHWLILHGRYVCQARKPKC
                     DICGIQDICLYYQNIIKPPQGPEP"
     gene            complement(354..1028)
                     /locus_tag="PK976_03255"
     CDS             complement(354..1028)
                     /locus_tag="PK976_03255"
                     /inference="COORDINATES: protein motif:HMM:NF013534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016977 - chitosanase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitosanase"
                     /protein_id="HRC66936.1"
                     /translation="MITPEIKRKILQVVNVFETGKLEGRYDALVVMNDGPGGIPQITF
                     GRSQTTEYSGLRDLILRYIKREGVYAAQLSEYEPRIGKTPLTNDQVFKQLLIQAGRND
                     PLMISVQDEFFDQTYYQPAFKFFTTNGFELPLSLLVIYDSYIHSGRVPDFLRRRFGEK
                     IPARGGNEKEWVTRYCDVRHQWLKYHSNPILRKTIYRTACFKEQIALGNWMLVLPIRV
                     QGLVAV"
     gene            complement(1041..1808)
                     /locus_tag="PK976_03260"
     CDS             complement(1041..1808)
                     /locus_tag="PK976_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66937.1"
                     /translation="MENNNGNSPTKDYMSARNVIAFLITLVILVATVYVAIVAVRISS
                     EQDRMEGLKFVAQTLLPLWGTWIGTILAFYFSRENFDAATKSYQNIIESMKPEEKIAK
                     IMAKDAMLPLEKITYLDYDETKTRTIRDILDDERFREYNRYAILNRDKTLKYIIHRST
                     FYRFLAEVSMGIVPIEKNTDDLTFSDMEEQASDAVKAMMYKGFNFIAENSTLLDAKKA
                     MDAIPECQDVFVTKSGKATEPILGLITNNKILDYARV"
     gene            complement(1820..2134)
                     /locus_tag="PK976_03265"
     CDS             complement(1820..2134)
                     /locus_tag="PK976_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66938.1"
                     /translation="MDLNFEDLIEGLSSILKNELEEGKEDVKTYARYIIEKRKQRLEQ
                     LAELYTRGFITKEELESELADEEKVIEAHLLSMQVMAKASIQQAANASIQFITDYLLK
                     LL"
BASE COUNT          640 a          500 c          442 g          699 t
ORIGIN      
        1 cgcgacgctg cttgaaaaat tcagtggtca ggtgcccgat aatctggatg atttggagag
       61 tttgcctggt gttggcagaa aaacggccaa tgtgattctt tcggttgcat tcaacaagcc
      121 ggccatgccc gtcgatacac atgttcatag agttgcaaga agaattggcc tcgtaaaaga
      181 tgcccgaact cccttggaaa ccgagaaaca attaactcgc tacattccag ctcattatat
      241 ctcaaaagct catcactggc ttattttaca tggaagatat gtatgccagg cacgtaagcc
      301 caaatgcgat atttgcggaa tacaagatat ttgcctgtat tatcaaaaca tcatcaaacc
      361 gccacaaggc cctgaaccct gataggcagc acaagcatcc agttgcccaa ggcaatctgc
      421 tccttaaaac aggccgtacg gtatatcgtc tttcgtaaga tgggattgct atgatacttc
      481 aaccattgat gcctgacatc gcaatatcgt gtaacccatt ctttctcgtt gcctccccgt
      541 gctggaatct tttcaccaaa tctacgcctc aaaaagtcag gtacccgtcc agaatgaata
      601 taactatcgt aaataaccag taaactcaag ggaagctcaa atccattggt agtaaaaaac
      661 ttgaaggctg gctgataata tgtctggtcg aaaaattcat cctgaacact gatcatcaaa
      721 ggatcattgc gcccggcttg tataagcaat tgtttaaaaa cttgatcgtt cgtaagcggt
      781 gttttcccaa ttcgtggttc gtactcactc aattgagctg catatacacc ttctcttttg
      841 atatacctca ggataaggtc tcttagtcca ctatattctg tggtctggct gcgaccaaaa
      901 gtaatctgag gaattcctcc tggaccatcg ttcatcacta ctagggcatc ataacgtcct
      961 tctaactttc cggtttcgaa aacgtttaca acctgcaaaa ttttgcgctt gatttcggga
     1021 gtaatcattt ttctaaggat ttacacacga gcataatcga gaatcttgtt gttggtaatc
     1081 aatcccagaa taggttctgt ggctttgcca cttttggtta cgaacacatc ctggcattct
     1141 ggaatggcat ccatggcctt tttggcatca agcaaggtac tgttctccgc aatgaaatta
     1201 aatcccttgt acatcatggc tttcacggca tcgcttgctt gttcctccat atcactgaaa
     1261 gtgaggtcgt cggtattttt ctcgattggg actataccca tcgagacctc agccagaaat
     1321 cgatagaaag tgctgcgatg gataatatat ttcaatgtct tatctcgatt gagtatagca
     1381 taacgattat attccctgaa cctctcatca tcgagaatat cgcgtattgt acgagttttg
     1441 gtttcgtcat aatcaaggta ggtaattttt tcgagtggaa gcatggcatc cttagccatg
     1501 attttggcta ttttttcttc gggtttcatg ctctcgatga tattttgata agattttgta
     1561 gcagcgtcga aattttcgcg actgaagtaa aaagccagaa tggtacctat ccatgttccc
     1621 caaagcggca aaagagtttg agccacaaat ttcaatccct ccatcctgtc ctgctcactc
     1681 gagatgcgaa cggctacaat agccacataa accgtggcca cgagaatcac aagggtaatc
     1741 aaaaaagcaa tgacattcct tgcactcatg taatctttag ttggactgtt gccattatta
     1801 ttttccatag ttaatctatt tataagagtt tcaataaata atctgtaata aattgtatgg
     1861 aagcattggc ggcttgctgt atggatgctt tggccataac ctgcattgac agtaaatgag
     1921 cctcaattac cttctcttcg tcggctaatt ccgattccaa ttcttccttg gtaataaaac
     1981 ctcgagtata aagttcggca agctgctcaa gacgttgttt ccgtttttct ataatatatc
     2041 gggcataagt tttcacatct tcctttcctt cttccaattc atttttcaaa atggatgaaa
     2101 gtccctcgat caaatcttca aaatttaagt ccatagcaaa aaaatttatt gaatggtttt
     2161 ggctttttcg gcattcagaa aggcaacaaa gagttcgtga aatctgtcgc gatggatagg
     2221 agcaagatgc aggttataag tattattttt tcgatgttgc tccttatatt tcacccagag
     2281 a
//
LOCUS       DAORPD010000207         2280 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_8563_length_2280_cov_2.791011, whole genome shotgun sequence.
ACCESSION   DAORPD010000207 DAORPD010000000
VERSION     DAORPD010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8563_length_2280_cov_2.791011"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1120
                     /locus_tag="PK976_03270"
     CDS             <1..1120
                     /locus_tag="PK976_03270"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRC66939.1"
                     /translation="HEIRISMNSILGMAELLYDAKLEKEQREWVEIINISGIKSLSFI
                     HDILDFSKIEAGFIKIESIEFNLSRIIEEVEPVLHFKANEKSILLSHSLETSLHQIEL
                     KGDAVRIRQVLINLINNALKYTSEGEMNIIGNIIFRNSDHVKFEIEVKDSGIGIPKAD
                     REKIFEGFNQLKLTDKNISEGTGLESRTGKSLVEMMDGMIGFGSEFGQGNNFWFTLYF
                     LLALEILSSLIQMIKKPRILLAEDIATNRKEIGSCSNQRGIYSYDLAIHGKQALENAK
                     DKKYDLMQMDIQMPQMDGIRATQHIRRHNPDDNPNVNTSILVITAYSTSADAYRFYHV
                     GMNVMMHKPFLFGEFPQTIENLLGEHFFQNKNRICKIN"
     gene            1122..1922
                     /gene="mazG"
                     /locus_tag="PK976_03275"
     CDS             1122..1922
                     /gene="mazG"
                     /locus_tag="PK976_03275"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HRC66940.1"
                     /translation="MNSENQQALEKFEQLLEIMDKLRAECPWDRSQTSESLRHLTIEE
                     TYELSDAILSHDGDAIRGELGDLMMHLVFYAKIASERGDFNLAEVLEGINRKLINRHP
                     HIFGDAVVTSAQEVADNWEKIKLEKEGKNSVLEGVPPSLPPLMKAYRMQEKARGIGFD
                     WENSQQVWEKVLEEMQELKQEVENLSPKERLEEEFGDLLFSLVNYARFIGVNPEDALE
                     NTNRKFISRFQYMEDVLNFNGQKFDQLTLNQMDKLWEEAKKSEKNQGK"
     gene            complement(2012..>2280)
                     /locus_tag="PK976_03280"
     CDS             complement(2012..>2280)
                     /locus_tag="PK976_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2400 family protein"
                     /protein_id="HRC66941.1"
                     /translation="KGVDFGLWYIPANKLLCPLDVHSGRTARELGLLSRPQNDLKAVI
                     ELTSHLRELDANDPIKYDFALFGLSMQKTSLWTSISGRPGDEEG"
BASE COUNT          733 a          399 c          499 g          649 t
ORIGIN      
        1 ccatgaaatt cgtatttcta tgaattccat tttagggatg gcggaattgc tttatgatgc
       61 caaattagaa aaagagcagc gcgaatgggt cgaaatcatc aatatatcgg gtattaaatc
      121 gctttctttt atccatgata ttttagattt ttcgaaaata gaggctggat tcatcaaaat
      181 tgaatcaata gaattcaacc tgtccagaat tatcgaagaa gttgaacccg tcttgcattt
      241 caaggctaat gaaaagagta ttctgctatc tcattcattg gaaacttctt tgcatcaaat
      301 cgaattgaaa ggggatgcag ttagaattcg gcaggttctt atcaatttga taaacaatgc
      361 cttaaaatat acttccgagg gtgaaatgaa tataattggc aatataattt ttcgaaattc
      421 tgatcatgtc aaattcgaga ttgaggtaaa agattcgggc attggaatac ccaaagcgga
      481 tagggaaaag attttcgaag gttttaatca gttgaaactt accgataaga acatatccga
      541 gggaacaggt ttagagtcga gaactggtaa gtctctcgtt gagatgatgg atgggatgat
      601 aggtttcggg agtgagtttg gtcaaggaaa caatttctgg ttcacccttt actttctatt
      661 ggctctggaa attttgtctt ctctcattca gatgattaaa aaacctcgaa ttttgctggc
      721 agaagatatt gcaaccaatc ggaaggagat aggttcctgt tcgaaccaga ggggtattta
      781 ttcttatgat ttggccatac acggaaagca agctctcgaa aatgcaaagg ataagaaata
      841 tgacctcatg cagatggata ttcaaatgcc ccaaatggat ggaataagag ctacacagca
      901 tattcgaagg cataatccag acgataaccc gaatgtcaat acttccatac ttgtcattac
      961 agcttatagc acttcagccg atgcttatcg tttttaccat gtcggaatga acgttatgat
     1021 gcataaacct tttttattcg gagaatttcc tcaaaccata gaaaatcttc tgggagaaca
     1081 tttctttcag aataaaaata gaatttgtaa aataaattga aatgaattct gaaaatcaac
     1141 aagcgcttga aaaatttgaa caacttctcg aaatcatgga taaacttagg gcagaatgtc
     1201 catgggatag aagtcaaacg agtgaaagcc tgaggcattt aactatagaa gaaacctacg
     1261 aactttccga tgctatactc agccacgatg gagatgcgat tcgtggtgaa ttgggcgatt
     1321 tgatgatgca tttggtattc tatgcaaaga tagcctccga aagaggtgat tttaatttag
     1381 ctgaggtgtt ggaaggcata aaccggaagc tcatcaaccg acatcctcat atttttggag
     1441 atgcggtagt taccagtgct caggaagttg ccgataactg ggaaaaaatc aaactcgaaa
     1501 aagaagggaa aaattcagta ttggagggag tgccaccttc tcttccgcct ttgatgaagg
     1561 cttatcgtat gcaagaaaaa gctcgtggca ttggtttcga ttgggaaaat tcgcaacagg
     1621 tttgggaaaa agtgctcgag gagatgcaag aattaaaaca agaagtcgaa aatttatccc
     1681 ctaaagaaag actcgaggaa gagtttggcg acttgttgtt ttctttggtc aattatgcac
     1741 gctttatagg agtgaatccc gaagatgccc tcgaaaatac caatcgcaaa ttcatcagcc
     1801 gttttcaata catggaggat gtgctaaact tcaatggtca aaaattcgac cagctcacat
     1861 tgaatcaaat ggataaactt tgggaagaag caaaaaaatc ggagaaaaac cagggtaagt
     1921 gaattctgaa atggagaatg gtgagatgaa agttaaaaat gagttcttat gggttttatg
     1981 gtgaagttaa agtctctcag tctctccgtc ttcaaccttc ttcgtctccc ggccttcccg
     2041 atatgctcgt ccaaagggat gttttttgca tacttagccc aaacagtgca aaatcgtatt
     2101 ttatcggatc gttagcatct aattccctca ggtgggaagt tagttctatt actgctttca
     2161 agtcgttctg gggtcgggat agcaggccta attcacgtgc ggtgcggccg ctatgcacat
     2221 cgagcgggca caataattta ttcgccggaa tataccacag gccaaaatca acacctttgt
//
LOCUS       DAORPD010000208         2275 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_8592_length_2275_cov_2.104505, whole genome shotgun sequence.
ACCESSION   DAORPD010000208 DAORPD010000000
VERSION     DAORPD010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8592_length_2275_cov_2.104505"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="PK976_03285"
     CDS             <1..390
                     /locus_tag="PK976_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HRC66942.1"
                     /translation="HGETDELFEETRQFIQSLPLSYMHVFTYSERSNTPAASLDGKVS
                     PQIKHQRSQMLHQYSAAKLDQFYKLCQHQWVNVLFESENTDGMMSGYSENYIRVETPF
                     REDFVNQIIHLPLEQRNVTGNYAYYPR"
     gene            417..1586
                     /locus_tag="PK976_03290"
     CDS             417..1586
                     /locus_tag="PK976_03290"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HRC66943.1"
                     /translation="MYPKISDLINDIFGTHINLPIQSYGMMMAIAFVVGGLILNSELK
                     RKEREGILKPHTKKIIEGLPATPIEIALSGFITFILGFKIIGIILDYNFFADHPQEFI
                     LSWKGSWLGGLLAGGFSAYYIWHSKEKKKLPKPKTKFVEIHPHQLTVNILFVAVVFGI
                     IGAKLFDVMEHLDELVRDPLGTLFSFSGLTFYGGLILAAIAVVFYARKYGIAFEHIAD
                     ATAPALILAYAVGRIGCQLSGDGCWGIPNTSPKPHWLSFLPNWLWAFNYPHNVINEGI
                     PIPGCEGQHCFVLQQPVFPTPLYETIICTLFFLVLWGIRKKLRIPGLLFGIYLILNAF
                     ERFFIEEIRVNRNYHIIGPIELSQAQIIAIAIFITGVVFSIVFYRKYKHLQTQRI"
     gene            1583..>2275
                     /locus_tag="PK976_03295"
     CDS             1583..>2275
                     /locus_tag="PK976_03295"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HRC66944.1"
                     /translation="MNFKEIAFEVESIAKEAARYILEQRKSLSESDVETKGLHNYVTY
                     IDKGSESLIIDHLKPLINNAGFIAEENTQNLRGKEFNWIIDPLDGTTNFIHGVPICSV
                     SIALMQNSEIVVGEVLEIFSGESFLAWKDGPALCNGKPVQVSRRSNVKEILLATGFPY
                     YDYDLLDQYLALFKHFMKNTRGLRRLGSAALDLAWVACGRFDGFYEYGLNEWDVAAGS
                     FIVERAGGKCSDF"
BASE COUNT          650 a          461 c          466 g          698 t
ORIGIN      
        1 catggcgaaa ccgacgagct tttcgaggaa acccgtcagt ttattcaaag tcttcctctt
       61 tcgtatatgc atgtttttac ttacagcgaa cgctccaaca cacctgctgc aagcctcgat
      121 ggcaaggttt caccccaaat aaaacaccaa aggagtcaga tgcttcacca gtactcagca
      181 gcaaaactcg accaattcta taaactatgc caacatcagt gggtaaacgt gttgttcgaa
      241 tcggaaaaca ctgatggcat gatgtcgggt tattccgaaa attatattcg cgtagaaacc
      301 cctttcagag aagattttgt aaatcaaatc attcatttac cactcgaaca aagaaatgtt
      361 acaggaaatt atgcctacta tcctcgataa atcatccttc aattcatttt tcatatatgt
      421 atcctaaaat cagtgatcta atcaacgata ttttcggaac tcatattaat ttgccaattc
      481 agtcgtatgg gatgatgatg gcaattgcct ttgtagtagg cgggctcatc ctcaacagcg
      541 aattgaagcg gaaagaaagg gaggggattt taaaacctca tactaaaaag atcatcgagg
      601 gcttaccagc cactcctatc gaaattgctt tgtctggttt cattacattc atacttggct
      661 tcaagatcat aggcattatt ctggattata atttttttgc cgatcatcct caagaattca
      721 ttctctcatg gaaaggaagc tggttgggag gattacttgc gggcggattt tctgcttatt
      781 acatctggca ttccaaagaa aagaaaaagc tgcccaaacc taagaccaaa ttcgtagaaa
      841 ttcatcctca tcaattaacc gtcaatatat tatttgtagc tgtggtgttc ggtatcatag
      901 gagccaagtt attcgatgtg atggagcatc tggatgagtt agtccgtgat cctttaggta
      961 ctttattttc atttagtggt cttactttct atggtggttt gattctggct gcgatagccg
     1021 tggtatttta tgccagaaaa tatggaattg cattcgaaca tattgcagat gcaacagcac
     1081 cagctttaat actggcctat gccgttggcc gaattggatg ccaactgtcg ggcgacggtt
     1141 gctggggaat tcccaatact agtcccaaac cccattggct ttcatttcta cccaattggt
     1201 tatgggcgtt taattatcct cataatgtta ttaatgaagg aattccaatc ccaggttgcg
     1261 aaggtcagca ttgcttcgtg cttcaacagc ccgttttccc tactccttta tacgaaacca
     1321 tcatttgcac cttgttcttt cttgttttat ggggaataag gaaaaaactc agaattcccg
     1381 gcttactgtt cggaatctat ttaatcttga atgctttcga gagatttttc atcgaagaaa
     1441 ttcgtgtcaa ccgcaattat catatcatag gcccgatcga attgagtcaa gctcaaatca
     1501 ttgccatagc cattttcatc actggtgttg tgttctccat tgttttctat cgcaaataca
     1561 aacatttaca aacccaacga atatgaattt taaggaaatt gccttcgagg tagaaagtat
     1621 tgccaaagaa gccgcccgat acatcttgga gcaaagaaaa tcattatctg aatcggacgt
     1681 cgaaaccaaa ggtttacata actatgttac ttatatcgat aaaggttccg aaagcttgat
     1741 catcgatcat ttaaagcctt tgattaacaa tgcaggattt atagccgagg aaaataccca
     1801 aaacctaaga ggcaaggagt ttaattggat tattgatccc ttggatggaa ctaccaattt
     1861 cattcatgga gttcccatct gttcggtgag cattgctttg atgcaaaata gcgagattgt
     1921 ggtaggcgaa gtgctcgaaa ttttttcggg agagagtttc ttagcatgga aagatggacc
     1981 tgccctttgc aatggtaaac ctgtacaagt ttcacgtcgt tcgaatgtta aagaaatttt
     2041 attagccaca ggatttcctt attacgacta tgatttactg gatcagtatt tagcactttt
     2101 taaacatttt atgaagaaca cacggggtct caggcgcttg ggctcggctg cgctcgacct
     2161 tgcctgggtt gcctgtggac gtttcgatgg attttatgaa tatggcttga acgagtggga
     2221 cgttgcggca ggttccttca ttgtggaaag agccggagga aaatgttccg atttc
//
LOCUS       DAORPD010000209         2272 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_8610_length_2272_cov_2.591340, whole genome shotgun sequence.
ACCESSION   DAORPD010000209 DAORPD010000000
VERSION     DAORPD010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8610_length_2272_cov_2.591340"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="PK976_03300"
     CDS             <1..822
                     /locus_tag="PK976_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRC66945.1"
                     /translation="QPIHVDIHLVDNDILDVEAFRKWRPDLTDADFILEDGKYVCGYE
                     IEKMSKSLHNVQNPDDLIEKYGADTLRIYEMFLGPLEQSKPWNTMGIEGVFRFLRKFW
                     KLFHDNHNHFYLSDSPADEKELKILHRTIKKVREDIERYSFNTVVSAFMIAINELTDL
                     KCNKRQILEPLIILISPYAPHIAEELWEMSGHNESITYAHLPDYKGEYLMETSFDYPV
                     SFNGKVRFNISLPLDLNNDEIEQAIINNEKTAYYLQGNKPKKIIVVPGKIINVVY"
     gene            complement(867..1562)
                     /locus_tag="PK976_03305"
     CDS             complement(867..1562)
                     /locus_tag="PK976_03305"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF001186.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HRC66946.1"
                     /translation="MMEKYAIIVAGGSGSRMKSVTPKQFALLAGKPVLLYSIEAFLKA
                     FEDIRIVIVIQQGMEAFWFTMKKEYQLSERILIVHGGTERFHSVKNGLEKVPDDVLVG
                     IHDAARPLIDAELIGKTFQIAEKKKNCIPAIFAHDSVRILDDVEISRPVPRERVQLIQ
                     TPQVFYSTPLKKAYRTSFHEQFTDDASIWEFAGNKVEFCEGSPFNLKITIPSDFALAE
                     ALLMARQKEAGSF"
     gene            complement(1601..>2272)
                     /locus_tag="PK976_03310"
     CDS             complement(1601..>2272)
                     /locus_tag="PK976_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HRC66947.1"
                     /translation="TSRGRTIRFLYDGPYEKFRKTLMQLGETPLPKIHQRPVETEDAK
                     RYQTIYAKYEGAVAAPTAGMHFTRNLMKRMEIKGIRFAEITLHTGVGNFREIEVEDLT
                     KHKMDSEEMFIEEEAAKIINEAKLSHRKVCAVGTTSLKALETAATINGKIKPYRGWSN
                     KFIFPPYEFSVPDAMISNFHLPLSPMLMVVCAFGGYDFVMKAYKEAIEKKYKFFTYGN
                     AMLII"
BASE COUNT          699 a          500 c          420 g          653 t
ORIGIN      
        1 caacccattc atgtcgatat tcacctggtc gataatgata ttctggatgt ggaggctttt
       61 aggaaatggc gtcctgatct caccgatgcc gactttattc tggaagacgg caaatatgtt
      121 tgtggttatg aaatcgaaaa aatgtcgaaa tctcttcata atgtccagaa tcctgatgac
      181 ttgatcgaga aatatggtgc cgataccttg agaatttatg agatgttctt aggtcccctt
      241 gaacagtcaa aaccatggaa taccatggga atagaaggag tattccgctt tttaagaaaa
      301 ttctggaaac tgtttcatga taatcataat catttttact tgagcgactc gccagccgat
      361 gaaaaagaac tgaaaatcct gcaccgaacc attaagaagg tgagggaaga tatcgaacga
      421 tactcattca atacagtggt cagtgccttt atgattgcca tcaatgaact aactgattta
      481 aaatgcaata agcgccaaat actcgagcca cttatcattc tcatttctcc ttatgctcca
      541 catattgccg aagaactctg ggaaatgagt gggcacaatg aaagcattac ctatgcccac
      601 ctccccgatt acaaaggaga atatctaatg gaaacctctt tcgattatcc tgtttcattc
      661 aatgggaagg ttcggttcaa tatttctctt cccctcgatt tgaacaacga tgaaatcgaa
      721 caagcaatca taaataacga aaagactgcc tattatcttc aagggaataa gcccaaaaaa
      781 atcatcgtcg ttccaggaaa aatcatcaat gtcgtttatt agcaaagcac gaaacttcaa
      841 gcccttttct ctgaaacaaa ttcaaatcag aaagaaccag cttccttttg gcgggccatc
      901 agtaaggctt cggccaaggc aaaatcagaa ggaatggtta ttttgagatt gaatgggctc
      961 ccttcgcaaa attctacttt attccccgca aattcccaga tgcttgcatc atcggtaaat
     1021 tgctcatgaa aagaagttcg ataagccttt ttaagtggag tggaataaaa cacctgaggc
     1081 gtttggatca attgaaccct ttcacgagga actggacgtg aaatttccac atcatccaaa
     1141 atccgcacag aatcatgagc aaatatggca ggtatacaat ttttcttttt ttcggcgatt
     1201 tgaaaagttt ttccgataag ttctgcgtca ataagtggtc gcgccgcatc atgaataccc
     1261 actaaaacat catcgggaac tttttcgagg ccattcttaa cggaatgaaa tctctcagtg
     1321 ccgccatgaa ctatcagaat cctttctgaa agttggtatt cctttttcat cgtaaaccag
     1381 aatgcttcca taccctgttg aatcacaatc acgatacgga tatcctcaaa ggcctttaaa
     1441 aaagcttcta tactatacaa caagactggt ttccctgcca gcaatgcaaa ttgtttggga
     1501 gtaacagact tcattctcga gccactaccc cctgcaacaa tgatcgcata tttttccatc
     1561 ataaatcttt aactctggaa ggaaatctca gaaaatgaga ttaaataatc aacatagcat
     1621 tgccgtaagt aaaaaattta tacttctttt cgatggcctc tttataggct ttcataacaa
     1681 agtcgtaacc accgaaggca caaacaacca tcagcatggg cgaaagagga agatgaaaat
     1741 tcgaaatcat cgcatcggga accgaaaact cataaggtgg aaaaatgaat ttgttcgacc
     1801 atcctcggta aggtttaatt tttccattga tagtagctgc ggtttcgagg gctttgagcg
     1861 aagtagtccc taccgcacaa actttacgat gagacaattt ggcttcattg attatcttag
     1921 ctgcttcttc ttcgataaac atctcttccg aatccatctt atgcttggtc aggtcttcca
     1981 cttcaatctc tctgaaattt cccacaccag tatgaagtgt aatctcggca aaacgtatcc
     2041 cttttatttc catccgcttc atcagattgc gtgtaaagtg cataccagca gtaggcgcag
     2101 ccactgcacc ttcatacttt gcataaatag tttgataacg ttttgcatcc tcagtttcca
     2161 caggtcgctg atgaattttg ggtaaaggtg tctcacccaa ctgcatcaga gtctttctga
     2221 acttttcgta aggcccatcg tataaaaatc ttatggttcg gcccctcgaa gt
//
LOCUS       DAORPD010000210         2270 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_8624_length_2270_cov_2.255079, whole genome shotgun sequence.
ACCESSION   DAORPD010000210 DAORPD010000000
VERSION     DAORPD010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8624_length_2270_cov_2.255079"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1972)
                     /locus_tag="PK976_03315"
     CDS             complement(<1..1972)
                     /locus_tag="PK976_03315"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HRC66948.1"
                     /translation="MIPVKLTLEGLFSYQQRQEINFRPLVEARLFGIFGPVGSGKSSI
                     TEAILYALFGDIERLNSREARNYNMMNLRSNRLWIDFIFNAGNPSQQYRITVEGRRKP
                     KNYHETESLDRKAYQKSNNTWLPIALEKIDEVIGLSYDNFRRTIIIPQGKFQEFLQLG
                     GADRIRMLKEIFQLEQFDLYNKAKNLFEKNHEILIQVNTRLESLSATPEAITTLQDEL
                     NTITAALLQERQKYSELQKRKDQQALLYEKNQQWQGLKLQFEQLNKEAEVMERNRNFI
                     EKYQMAESSFRSPLDLLDEISNRVNSLEKRCKELSHQIEILQPQRNNIQNQLALWKQK
                     SEELPFREVFVEKIEELLHYRKIQQQIIEINPKIQALIDQIKSHDETLKSKKEEKSSI
                     LKEYEELETQLPNPNEIKQINQWFTEYDRLLEAKQQTEVRKQSALNDMEIIKQDALQN
                     SHLILCHELLAQKSIPEVLEEIQRNYNLSKNQLDEIRNQIQSLKVTTKLADFAKELRE
                     GMPCPLCGSTSHPHLFSAPEVEAKLKKLESTEIQLKAQQQAIIDAMSELKQYLGKLKA
                     KSDEIEKANRGLEKLTQQIDLHFNTYHFDGLEVGNKKAYQEFSEKIEFLQNRLRKLNL
                     NRNTLEQEIHNLEEKHRKQSTEMNQLQQQINFL"
     gene            complement(1969..>2270)
                     /locus_tag="PK976_03320"
     CDS             complement(1969..>2270)
                     /locus_tag="PK976_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66949.1"
                     /translation="FQWLPHHTDCLLEMTLHTDIDLNAEITNHFHRLHPGIVQIIPEF
                     PEESNLKEKEEIAPDPTGEIMELFKDYFRSIKQVPPDEETLDIFREIIANEGDNQ"
BASE COUNT          563 a          405 c          455 g          847 t
ORIGIN      
        1 aaagaaaatt tatttgctgc tgcaattgat tcatttcagt gctttgtttt ctatgttttt
       61 cttctaagtt atgaatttct tgttctaatg tatttcgatt caagttaagc tttctcagcc
      121 gattttgtaa aaattctatt ttttctgaaa attcctgata agccttttta ttaccaactt
      181 ctaagccatc gaaatgataa gtgttgaaat gtaaatcaat ttgttgagtt aatttttcta
      241 accctcggtt agccttttct atttcatctg attttgcttt cagcttgcca aggtattgtt
      301 tcagttcact catagcatcg ataatggctt gttgttgagc ttttagctgt atttctgtgc
      361 tttcgagttt cttcaatttt gcttcgacct caggtgcgct aaaaagatgt ggatgtgagg
      421 tagacccgca gagtggacaa ggcattcctt cccgaagttc ttttgcaaaa tcggctaatt
      481 tagtggttac ttttaaggat tgaatctgat tcctaatttc atctaattga ttcttagata
      541 aattatagtt tctctgtatt tcttccaaaa cctctggaat agatttttga gccaataact
      601 cgtggcataa aatgagatga gaattttgca gggcatcttg ttttatgatt tccatgtcgt
      661 tgagcgccga ttgctttcgt acctctgttt gctgtttggc ttccaaaagt ctatcatatt
      721 ctgtgaacca ttgatttatt tgtttgattt cattggggtt gggaagttgg gtttcgagtt
      781 cttcatattc tttgagaatg gaggattttt cttctttttt cgacttgagt gtttcatcat
      841 gcgatttgat ttgatctatt aaagcttgaa tcttaggatt gatttcaata atctgctgtt
      901 gaatttttcg ataatgcaaa agttcttcta tcttttcaac aaaaacttcg cggaatggaa
      961 gttcttccga tttctgtttc cataaagcaa gctgattttg gatattattc ctttgaggtt
     1021 ggagtatctc tatttgatga gaaagctctt tgcatctttt ttcaagtgaa ttcaccctat
     1081 tcgatatctc gtcgagcaag tccaaagggc tgcggaagga ggattcagcc atttgatatt
     1141 tttcgatgaa attacggttt ctctccataa cctcggcttc cttgttcagc tgctcgaatt
     1201 gaagtttcaa accttgccat tgttgatttt tttcgtaaag aagcgcctgc tgatcttttc
     1261 ttttttgaag ctcagaatat ttttgtcgtt cttgcaatag tgccgcagta atcgtgttta
     1321 attcgtcctg aagagtggta attgcttcgg gagtggcgct taagctttcg aggcgagtgt
     1381 taacctgaat caggatttcg tggtttttct cgaataagtt tttggccttg ttgtagagat
     1441 caaattgttc gagctgaaag atttctttta gcattcttat tcggtcggcg ccgccaagct
     1501 gaaggaattc ctgaaatttt ccctgaggaa taataatagt acgacggaaa ttatcgtaag
     1561 aaagaccgat tacctcatca attttttcta aagcaatggg gagccaagta ttgttggatt
     1621 tttggtaagc cttgcgatcg agagattctg tttcgtgata attttttggc tttcgccggc
     1681 cttccacagt tatcctgtat tgttgggaag ggttcccggc attaaagatg aagtcaatcc
     1741 acagacggtt cgaacgcaga ttcatcatgt tgtaattacg ggcttcgcgt gaattcaatc
     1801 gttctatatc gccgaaaagg gcatataata tggcttcggt tatgctcgat tttccgcttc
     1861 ctactggtcc aaaaatacca aacagtctgg cttcaaccaa tggcctgaaa ttgatctcct
     1921 gcctctgctg ataactgaat aaaccctcta aagttaattt tactggaatc attgattatc
     1981 cccctcgttt gcaataattt cacgaaatat gtctaaagtt tcttcgtcgg gcggcacctg
     2041 tttgatgctt cgaaaataat ctttgaataa ttccattatc tctccagttg gatcgggagc
     2101 aatttcttcc ttttctttca gattggattc ttcaggaaat tcaggaatga tctgaactat
     2161 tccgggatgg agtcgatgaa aatgatttgt gatttctgcg tttaaatcaa tatcggtatg
     2221 tagagtcatc tcgagaaggc aatccgtatg atgaggaagc cattgaaaag
//
LOCUS       DAORPD010000211         2269 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_8636_length_2269_cov_2.030262, whole genome shotgun sequence.
ACCESSION   DAORPD010000211 DAORPD010000000
VERSION     DAORPD010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8636_length_2269_cov_2.030262"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            386..1078
                     /locus_tag="PK976_03325"
     CDS             386..1078
                     /locus_tag="PK976_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRC66950.1"
                     /translation="MEASAYKILVVDDEPDVLEFLGYNLQREGFLVFTANNGFEAIEK
                     VKESKPHLVLLDLMMPGMDGMETCQELRKIVSGQDMLIVFLTARGEDYSQIAGFEMGA
                     DDYVTKPIKPKVLISRIKALLRRIQPAELEKIIRFEGLTIDRERYVVIQNDEEIILPR
                     KEFELLSLLASKPDRVFTREEIFEQVWGNDVIVGERTIDVHVRKIREKLGISIITTVK
                     GIGYKFENIGDE"
     gene            1124..2179
                     /locus_tag="PK976_03330"
     CDS             1124..2179
                     /locus_tag="PK976_03330"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRC66951.1"
                     /translation="MKKIFRFSDSRKVASLTAFSTIVAYGIFLVIINLIYQQPVGIQF
                     LLLGLGIWVAVYFITLFFVDKFIFSKIRVIYKTIHSQKVPKGIDPRQTIEAHSVEDVQ
                     REVTEWSDERREEIETLKELEKYRREYIGNISHELKTPIFNIQGYILTLLDGGLEDPN
                     VNRNYLLKAEKSVNRMIAIIEDLEMINLLEANELKLNIIRFNLVDLAREAVESLEMKT
                     KKRECEVFINHNIDRPIYVLADRQRILQVFTNLIDNAIKYGKKSGGRIKISFFDMDEN
                     ILAEITDDGPGISQNDLTHIFERFYRLDRGRSREMGGTGLGLAIVKHIIEAHHQTVNV
                     RSSIGVGTTFGFTLKKA"
BASE COUNT          694 a          395 c          483 g          697 t
ORIGIN      
        1 agccctgtta atcaggcttt ttatcttacc gatgaaagca tcataaacct cttgattctt
       61 ctgataattt tctcttaccc tgtcaattct tacttgcagg cgctcggatt gccccttgaa
      121 ttcttctctt cgtgaacgtc tttccgcttc ttccaaccag ttcgacatgc acttttgctt
      181 ttggtcaact atcgcattaa ttccttgcaa atttaattat tttaaaggcc ttggttattt
      241 aaacccaaaa agattgtaac aaaaatttca ttcctttata catttgtttt tatatataaa
      301 gtctttgtta atcttgtttc aagataaata gattacattt gcccagagca attcgcatga
      361 aaaagtagaa acacataaat tacaaatgga agcttcggca tataagattt tagtggttga
      421 cgacgagccc gatgtccttg agtttttagg atacaattta cagcgagagg gttttctcgt
      481 ttttacagcc aataatgggt tcgaagcaat tgaaaaggtg aaggaaagca agcctcattt
      541 ggttttgctc gatttgatga tgcctgggat ggatgggatg gaaacctgtc aagaacttcg
      601 aaaaattgtt tctgggcaag atatgttgat tgtttttctc accgccagag gcgaagatta
      661 ctctcagata gccgggtttg agatgggtgc cgacgattat gttacgaaac ccattaaacc
      721 caaagtattg atcagtcgga ttaaggcctt gctacgtcgt attcagccag ctgaattgga
      781 aaagataata cggttcgaag gtttgaccat tgatagagaa cgatatgtag taatacagaa
      841 tgacgaagaa atcatcttgc caaggaaaga attcgaattg cttagcctgc tggcttcgaa
      901 accagacaga gtctttaccc gtgaagagat ttttgaacag gtatggggca atgatgttat
      961 tgtaggggaa agaacaatcg atgttcatgt gcggaaaatc agggaaaaat tgggaatttc
     1021 gattattacg accgtaaaag ggattggtta taaattcgaa aatataggtg atgagtgagg
     1081 atgattgttc tttcgataaa attatgagtt ttgttcctga tatatgaaga agattttccg
     1141 gttttccgat tcccgtaaag ttgcttccct tactgctttt tcaacaattg tggcttatgg
     1201 cattttttta gtcattatca atttaatata tcaacaaccc gttggtattc aatttcttct
     1261 attaggcctt ggaatctggg tagccgtata ttttatcacg cttttctttg tggataagtt
     1321 cattttcagt aagatcagag tcatttataa gaccatccat agtcaaaagg ttcccaaggg
     1381 aatagacccc aggcaaacca tcgaagccca ttctgttgaa gatgtgcagc gtgaagtaac
     1441 cgaatggagc gatgaacgta gggaagaaat agagactttg aaggagctcg aaaaataccg
     1501 gagagaatat ataggtaaca tttctcatga attgaaaact ccgattttta acattcaggg
     1561 atatattctt acgttgctcg atggagggct cgaagatcca aacgtgaacc ggaactatct
     1621 tttgaaagcc gaaaagagtg taaatcggat gattgccatc attgaagatc tcgaaatgat
     1681 caatttattg gaagcaaatg aattgaaact caatataata cggttcaatc tcgtggattt
     1741 agcaagggaa gccgttgaat ctctcgagat gaagactaag aaaagagaat gtgaagtgtt
     1801 tatcaaccat aacatagacc gccccattta tgttttggcc gacaggcaaa gaatacttca
     1861 ggtttttacc aatttgatcg ataatgctat aaaatatggt aaaaaatcag gagggcgaat
     1921 caagataagt tttttcgata tggacgagaa cattttggct gagattaccg atgatgggcc
     1981 gggtatatca cagaatgatc taactcatat attcgagaga ttttaccgcc ttgacagagg
     2041 tcgttcgaga gaaatgggcg gcacgggact tggactggcc attgtaaagc atatcatcga
     2101 agcccatcat caaaccgtga atgtgcgtag cagcataggc gtgggaacta ccttcggctt
     2161 tacattgaaa aaagcctgaa ccttgctaag aattcttacc ggcaattaca tttcatcctt
     2221 attcgagaat caactaattt ttcttctctt tttgatgttt cgatgaaga
//
LOCUS       DAORPD010000212         2267 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_8648_length_2267_cov_2.494575, whole genome shotgun sequence.
ACCESSION   DAORPD010000212 DAORPD010000000
VERSION     DAORPD010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8648_length_2267_cov_2.494575"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="PK976_03335"
     CDS             <1..459
                     /locus_tag="PK976_03335"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRC66952.1"
                     /translation="GPEYRAAAASLTRHLVDSLQNLYPQAKVIIMGDLNDDPTDPSVL
                     TVLNAKGDKREVQPGDLYNPMWKLFKNGVGSLAYRDNWNLFDQIILTYPLINQENNGF
                     KFYTARVFNKKFLFNKEGQYKGYPFRTFAGGAYQGGYSDHLPVYIILIKE"
     gene            487..>2267
                     /locus_tag="PK976_03340"
     CDS             487..>2267
                     /locus_tag="PK976_03340"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HRC66953.1"
                     /translation="MVHRFSLKTFSVKFLIFSVFQISSLAVFAHQDSINFIPAIPVDI
                     QPNFITIEWQTSNDVFSGVYYEAEGKNAWILDVATKPLRNHSYNIPASYPSQVIYALP
                     FQFVGSDTLWGDTLVLMSQSASSGKMRVYFTRPIDPNVATGSQAIYLPGTAADTVIEL
                     IHRAKNSIDLAIYNMNIDGIAYALNQAKVRGVRVRVIYDGSTTNSSIYVLQGVPILAS
                     PTSSSYGIMHNKFMVIDCDTDDPNDAILFTGSMNFTSTQVNKDANNILIIQDRSLALA
                     YRMEFEEMWGGSGNQPNPSASRFGPFKKNNTPHYFNVGGIPIESWFSPSDGVNSKILA
                     AINSANTSLFIPTNLITRDDLANAIAKRRQNGVLCRVVVDDDASCSDQVVATLTQALG
                     TNFRETTESGILHHKVLIADALQTNSNPVVLTGSHNWSNNAEIRNDENTLILYDANIA
                     NLYYQEFSQRFKQSRVIAPNPILDLGPDISVCAGQKVILDAGTGFFSYLWSNGSTASQ
                     IIVDTSGMGLGSITLSCTVNNVYGTQTDQITITFFTCTSIEDNLTKRQFSIYPNPTRE
                     FFKMQLPPDVKPQFIEVYGIQGQMVMR"
BASE COUNT          671 a          461 c          440 g          695 t
ORIGIN      
        1 ggtccagaat atagagctgc agccgcctct ttaacgcgtc atctggtcga ttcactccaa
       61 aatctctatc cacaagcaaa agtcatcatt atgggcgatt taaacgatga tcccaccgat
      121 cctagcgttt taacggtact gaatgcaaaa ggagacaaaa gagaagttca acctggcgat
      181 ttatacaatc ccatgtggaa attattcaaa aatggagtag gatcattggc ttatcgtgat
      241 aactggaatc tgttcgatca gatcattctc acttatcctt tgataaatca agaaaataat
      301 ggttttaaat tttatacggc tcgagtcttc aataaaaagt ttttatttaa taaagaagga
      361 cagtataaag gctatccttt tcgcaccttt gccggtggtg cctatcaagg cggttattct
      421 gatcatttac cggtttatat tatcctcatc aaagaataaa gtttttcaac ctaaaatttt
      481 ttcctaatgg tgcatcgttt ctctttaaaa accttttcag taaaatttct aattttttct
      541 gtttttcaga tatcttcatt agctgttttt gcccatcaag acagtataaa ttttattcct
      601 gcaatacctg tagatattca acccaatttt atcactattg aatggcaaac ttcgaatgat
      661 gttttttcag gggtttatta tgaagcagaa ggcaagaatg cttggatttt ggatgttgca
      721 actaaacctc tgcggaatca tagttacaat attccggcgt cttatccttc gcaagtgatt
      781 tatgctcttc cttttcaatt cgttggaagt gacacgcttt ggggtgatac attggtgctg
      841 atgtctcagt cggcttcttc ggggaagatg agggtttatt ttactcgtcc tatcgatccc
      901 aatgttgcca ctgggtctca agccatatat ctgcccggca cggctgccga tacggtaatc
      961 gagctcattc atcgagccaa aaatagtata gatctggcta tttacaatat gaatatcgat
     1021 ggcattgctt atgccttgaa tcaggcaaaa gtacgagggg tgagagtaag agtgatttac
     1081 gacggaagca caaccaacag ctcgatttat gtacttcaag gcgtgcccat acttgctagc
     1141 cctacttcat cctcttacgg aatcatgcac aataaattca tggttatcga ttgcgataca
     1201 gacgatccta acgatgcaat tctttttaca ggaagtatga actttacttc aactcaggtt
     1261 aacaaggatg ccaataatat tttgattatt caggacagaa gtttggcatt agcttatcgc
     1321 atggagtttg aagaaatgtg gggaggctct ggcaaccaac ctaatccatc tgcctcgcgt
     1381 tttggacctt ttaagaaaaa taatactcct cattatttta atgtgggtgg aattccaatc
     1441 gaaagctggt ttagcccttc cgatggagta aattcaaaaa tccttgcagc tatcaacagc
     1501 gctaatacca gcttatttat tcctaccaat ttaattactc gtgatgatct tgccaatgct
     1561 atcgcgaaaa gaagacaaaa tggagtactt tgtcgagtgg tggtagatga tgatgcctca
     1621 tgttcggatc aagtggtggc tacacttact caggctttgg gaacaaattt tagagaaact
     1681 actgaaagtg gtatccttca ccataaagtc ttgattgctg atgccctaca aaccaactcc
     1741 aatcctgtgg tactcaccgg ctctcataat tggagcaata atgccgaaat tagaaacgac
     1801 gaaaataccc ttatacttta tgatgccaac attgccaatc tatactacca ggaattctct
     1861 cagcgtttta aacagtcgag agtgatagcc ccaaacccca tccttgactt aggacccgat
     1921 ataagcgttt gtgctggcca gaaagtaata ttggatgcgg gaacaggatt tttttcctat
     1981 ttgtggtcaa atggcagcac ggcaagccag ataattgtgg atacttccgg aatgggtctg
     2041 ggaagcatta cattgagctg cactgttaac aatgtctatg gtacgcaaac cgatcaaata
     2101 accattacct tttttacatg tacaagcata gaagataatc tcacgaaaag acaattttct
     2161 atttatccca atcctacccg ggaatttttc aaaatgcagt taccgcctga cgttaaacca
     2221 caatttattg aagtttacgg cattcaagga caaatggtaa tgcggca
//
LOCUS       DAORPD010000213         2255 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_8697_length_2255_cov_2.388182, whole genome shotgun sequence.
ACCESSION   DAORPD010000213 DAORPD010000000
VERSION     DAORPD010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8697_length_2255_cov_2.388182"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..334)
                     /locus_tag="PK976_03345"
     CDS             complement(59..334)
                     /locus_tag="PK976_03345"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HRC66954.1"
                     /translation="MTALQITISGRVQGVGFRYYIFRAAQELRIKGIIKNLPDGKVYI
                     EAEGDPQNIEIFLEKCREGPPLAYVTHIHITECLPSGYNDFRVAYGY"
     gene            complement(394..1830)
                     /locus_tag="PK976_03350"
     CDS             complement(394..1830)
                     /locus_tag="PK976_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66955.1"
                     /translation="MAKTELSKSTFIRALQCLKSVYLNKNRPFLRDHLEARQLNKFER
                     GHEIGSLAWKLFPGGIDCSPKHPAAYAKAAAKTTELIRRGATILYEAVFEYDGIRVLP
                     DILVLNDSQIRMYEVKGSLNISQTYILDAALQYYVISGAGYTPSQTFILHVNKNYIYE
                     GGEIHPEDFFVFQDVTAEVIQQQNFIELKIPEAKAAVNADKSPEIPIGPHCYLPYPCD
                     FMGLCWKSIPADSPLYLNSIDIHSRFRLVSEGYSSISRIPLDSLDNELLKTEVEARIN
                     GKPIYKASELQKLLQPQKHQVYLKILFIENAVPRLAGTRPFEPLPIAWIAVKENGSLI
                     MGDSENQYQVFSEIINLLRNFLTEDDQLVFWGKPMELLIHQVEFDLPFLNLESVFQSE
                     NLYLPFYLEEKTPENIASQLGIINDEITTMNDDEAIRAWSGESSKGKFNSREIENHLK
                     MQLFLLRELYRYAAGFIETDHSIEAKKY"
     gene            complement(1848..>2255)
                     /locus_tag="PK976_03355"
     CDS             complement(1848..>2255)
                     /locus_tag="PK976_03355"
                     /inference="COORDINATES: protein motif:HMM:NF021613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HRC66956.1"
                     /translation="RMNSTQPYLLAAHNGKEFDFPYISRRMLVNGIMLPDVFDNSGKK
                     PWETSFIDTMEMWKFGDYKHYTSLALLAALFDIPSPKEDIDGSQVAIVYYESNDLKRI
                     VRYCQKDVITVARLYCKFKGLPAPADGQIVIIE"
BASE COUNT          632 a          443 c          473 g          707 t
ORIGIN      
        1 gagaaaaata ttattttttt tttcatttct cgcaactttc gttggaaatg ttaatttttc
       61 aatatccata agcgacgcgg aaatcgttat aaccagacgg taggcattcg gtaatatgaa
      121 tatgagtaac ataagccagg ggagggcctt ccctgcattt ttccagaaat atttctatgt
      181 tttgagggtc gccttctgct tcgatgtaaa ctttaccatc gggaaggttt ttaatgattc
      241 ctttaattcg aagttcctga gctgctctaa agatgtagta cctgaaccct actccctgaa
      301 ctcgtcctga aatggtgatt tgcaaggctg tcattctcta tgatctggtc gaataatgat
      361 agcgaataat cgaaattttg ttcaagttca ttgttaatac tttttggctt caatagaatg
      421 atctgtttcg atgaaaccag cagcatacct atacaattct ctcaataaaa ataattgcat
      481 tttcaaatga ttttcgatct cccgggagtt gaattttccc ttcgatgatt cacccgacca
      541 ggctcttatc gcctcgtcat cgttcatggt tgtgatttcg tcgttgataa ttcccaactg
      601 tgaagcaata ttttcgggtg ttttttcttc gagataaaat ggcagataaa gattttccga
      661 ttgaaagaca gattcgagat ttaagaatgg gagatcgaat tctacctgat ggatgagaag
      721 ctccataggt tttccccaaa acacgagttg atcatcttca gtcagaaaat ttctgagcag
      781 gttgatgatt tccgagaaga cttgatactg attttcggaa tcgcccatga taagacttcc
      841 attttccttc acggctatcc aagctatagg aagaggctcg aaaggacgcg ttcctgcaag
      901 ccgtggcact gcattttcga tgaacagaat cttcaaataa acctgatgtt tttgaggttg
      961 caataatttc tgcaattcag atgctttgta tataggcttg ccatttatcc ttgcttctac
     1021 ttcagttttc agcagttcat tatctaaact atccaaaggg attcgagaaa tagaagaata
     1081 tccttctgag acaagccgaa aacgggagtg aatgtcgatg ctgttgagat agagtgggct
     1141 gtcggctggg atcgatttcc agcacaagcc catgaaatcg caggggtaag gtaaataaca
     1201 atgaggtcct atgggaattt caggtgattt atcagcatta accgctgctt tagcttctgg
     1261 aatctttaat tcgataaaat tttgttgttg aataacttcg gcagtaacat cttgaaaaac
     1321 aaaaaaatcc tctggatgaa tctcacctcc ctcataaata taattcttgt tgacatgaag
     1381 aataaaagtc tgtgaaggag tataacccgc tccactgatg acataatatt gcaatgcggc
     1441 atcgaggata tatgtttgtg aaatgttcaa cgatcctttc acctcataca ttcgtatctg
     1501 tgaatcgttc aacacgagga tatcggggag aacccgtatg ccatcgtatt cgaaaacagc
     1561 ttcgtaaaga attgttgctc ctctgcgaat gagttcagtg gttttagctg ctgctttggc
     1621 ataagccgca ggatgttttg gagagcaatc tatgccacca gggaaaagtt tccaggctaa
     1681 tgaaccgatt tcatgacccc gttcgaattt gtttagttga cgtgcttcga gatggtctct
     1741 caaaaatggc ctgttcttgt tcaaataaac cgatttgaga cattgtaatg ctctgataaa
     1801 tgttgacttc gataattccg tttttgccat ataattgagg tttcgggcta ttcgataatg
     1861 actatctggc cgtcggcagg agctggcaaa cctttgaatt tacagtaaag tcgcgcaaca
     1921 gtaattacat ccttctggca ataccgcact atgcgtttca aatcgttcga ttcataatat
     1981 actatggcta cctgactgcc gtcaatatct tccttgggtg aaggaatgtc gaagagagcc
     2041 gccagaagag cgaggctggt ataatgttta taatcgccaa atttccacat ctccatggta
     2101 tctatgaaac tggtttccca gggtttttta cccgaattgt cgaaaacatc tggtagcatg
     2161 attccattta ctagcatacg ccttgaaatg taagggaaat cgaactcctt gccattgtga
     2221 gcggcaagca gatagggttg agtgctgttc atccg
//
LOCUS       DAORPD010000214         2243 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_8792_length_2243_cov_2.204296, whole genome shotgun sequence.
ACCESSION   DAORPD010000214 DAORPD010000000
VERSION     DAORPD010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8792_length_2243_cov_2.204296"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="PK976_03360"
     CDS             <1..207
                     /locus_tag="PK976_03360"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRC66957.1"
                     /translation="DNFVLQPQIYSKRVNAHPIEIFLIMMMGGSVGGIIGMILAVPAY
                     TLIRIVGKEFLSSFDLMDKLTRNL"
     gene            219..1220
                     /locus_tag="PK976_03365"
     CDS             219..1220
                     /locus_tag="PK976_03365"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HRC66958.1"
                     /translation="MRIFSWIRLIRCLTFRRSWNFLKNYASYSISQWFDRYIHWGFPW
                     TLSIEASGICNLHCPECPVGSHSLTRSAGLLRLHDYAQWLDESSPELFYLILYFQGEP
                     LLNEDFAAMVKMAHQKRIYTITSTNAHFLTSELSENIIMAGLDEIVISLDGTDSLTYE
                     KYRKGGSFDKATEGIRQLVAARKRSNRKNPFIRLQFIVMKHNQHQINKIQSLGKQLGV
                     DAVELKSAQIYSPGDENGLLTDIPEFARYIEGEDSLLLMKNRRKRGCRRMWQGSVITW
                     DGRLIPCCYDKDAVHSYGLLDSSTQVKTIWTSPNATIFRQNVMKSRDVYEICRNCGE"
     gene            complement(1263..1829)
                     /gene="frr"
                     /locus_tag="PK976_03370"
     CDS             complement(1263..1829)
                     /gene="frr"
                     /locus_tag="PK976_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HRC66959.1"
                     /translation="MLSEESEFCLEETRESMDNALLHFEKEFQKIRAGKATPQMLDGV
                     KVDYYGAITPIDKISNITTPDPRQIIVQPWDKTMLALIEKAILAANLGFNPQNNGEVL
                     RIIVPPLTEERRRELVKKAKVEAENAKVTIRNIRRNAMETAKKLKKDGIPEDEIDQLE
                     EEIQKITDAYIEKIDKAFEIKGKDIMTV"
     gene            2033..>2243
                     /locus_tag="PK976_03375"
     CDS             2033..>2243
                     /locus_tag="PK976_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HRC66960.1"
                     /translation="MKAYLFPGQGAQFSGMGKDLYEKSELARELFEKANSQLGFRITD
                     IMFQGSEDELMQTKVTHPAIFLHSVI"
BASE COUNT          623 a          418 c          460 g          742 t
ORIGIN      
        1 gataattttg tgctgcaacc tcagatttat tctaaaagag taaatgctca tcccatcgag
       61 attttcttga ttatgatgat gggcggttcg gttggaggga taataggaat gattctggcc
      121 gttccggctt atactttgat tcgtatagtg ggaaaggagt ttttgagcag tttcgacctg
      181 atggataaac ttacacgaaa tctttgatga tgaatttgat gcgcattttt tcatggatca
      241 ggctcatacg ttgcctcact tttcgacgtt cttggaattt cctgaaaaat tacgcaagtt
      301 attccatctc ccaatggttt gatagatata tccattgggg ttttccatgg acactttcta
      361 ttgaagcttc gggtatatgt aatctgcatt gtcccgaatg tcctgtcggc agccatagcc
      421 ttacgcgttc ggcaggcttg cttcgccttc atgattacgc acaatggctc gatgaatctt
      481 ctcccgaact tttttatctg attctgtatt ttcagggtga acctcttttg aacgaagatt
      541 ttgctgctat ggtaaaaatg gctcatcaaa agagaattta taccatcact tctaccaatg
      601 cacatttttt aacttccgaa ctgtctgaga atattataat ggcaggtttg gacgagattg
      661 tgatttcact cgatggaacc gattccctta cttatgaaaa atatagaaaa ggtggaagtt
      721 tcgataaagc caccgaagga atacggcagc ttgtagccgc ccgaaaacgt tcgaatcgaa
      781 aaaatccttt tatcaggtta cagtttatcg taatgaaaca taaccaacac caaataaata
      841 aaattcaaag cctggggaaa caattagggg tcgatgcggt agaacttaaa tcggctcaaa
      901 tctacagtcc tggggatgag aatggtcttc ttacggatat acctgaattt gcacgatata
      961 ttgaaggaga agatagttta ttgctcatga agaaccgtcg taaaagaggc tgcaggagaa
     1021 tgtggcaagg gtcagtgatt acctgggatg gtagactgat tccgtgttgt tatgataaag
     1081 atgctgtcca ttcttatggg cttctcgatt cttcaactca ggttaaaact atatggacaa
     1141 gcccaaatgc tacaatattt cgtcaaaatg tgatgaaatc gagagatgtt tatgagattt
     1201 gtcgtaactg tggggaataa aaacgaaaca atggagtgag gtaaaaattt tagactttcg
     1261 actcacacag tcataatgtc ttttcctttt atttcgaatg ctttatcgat tttttcgatg
     1321 tatgcgtcgg ttattttctg gatttcttct tccaactgat cgatttcatc ttcgggtatg
     1381 ccatcttttt tgagtttttt tgcagtttcc atggcattgc gacgaatatt gcgaatcgta
     1441 acttttgcat tttcggcttc aacttttgcc ttttttacca gttccctgcg cctttcttcg
     1501 gtaagaggtg gaacgatgat tcgcagaacc tctccgttgt tttggggatt gaaaccaaga
     1561 tttgcagcaa gaattgcctt ttcgatgagt gcaagcatcg ttttgtccca cggctgaaca
     1621 atgatctggc gaggatcggg agtggtaatg ttgcttatct tgtcaatagg agtgattgct
     1681 ccgtaataat ccactttcac tccatcgagc atctgaggtg tggctttgcc tgccctgatt
     1741 ttctgaaatt ctttttcgaa atgcaaaagg gcattatcca tgctttcacg tgtttcttcc
     1801 aaacaaaatt cgctttcttc gcttaacata aataatcttc cattgatttt cgattgcaaa
     1861 aatataatat ttgccaatct ggtcgaaatt tacatagaag gattcggctc gaaaaatatt
     1921 cttacttttg ccgcctcgtt tcaaaattat taagaaatag tatatttctg aatttgaata
     1981 gtaaatttag aagaatcgaa tttcacttta atttaacata acctatcatt tcatgaaagc
     2041 atatcttttc cctggacaag gggctcaatt tagcggtatg ggcaaagatt tatatgaaaa
     2101 atcggagctt gcgcgcgagc ttttcgaaaa agctaattct caacttggtt ttcgaattac
     2161 cgatattatg tttcagggta gtgaagacga actcatgcag accaaagtta ctcacccagc
     2221 catattccta cacagtgtta tat
//
LOCUS       DAORPD010000215         2239 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_8813_length_2239_cov_2.028388, whole genome shotgun sequence.
ACCESSION   DAORPD010000215 DAORPD010000000
VERSION     DAORPD010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8813_length_2239_cov_2.028388"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..91)
                     /locus_tag="PK976_03380"
     CDS             complement(<1..91)
                     /locus_tag="PK976_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRC66961.1"
                     /translation="MMRLDSKVAIITGASSGIGKAIALELAKEG"
     gene            complement(96..>2239)
                     /locus_tag="PK976_03385"
     CDS             complement(96..>2239)
                     /locus_tag="PK976_03385"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HRC66962.1"
                     /translation="AENIILKTSTTILKIGKNPVRFTFLTLDGRIINGDDPALGTNWN
                     GTEVTTCKILQAGEHFIGLGEKTGNFDRRGNAYWHWNTDNPRYDEYSDPLYSTIPFYI
                     GLHSGLTYGIFFDNTYKSYFNFGASQERFSSFGADDGEMDYYFINGENVAEIIKNYTW
                     LTGRMELPPIWALGNQQCRWSYYPDTEVLEIASMFRDKKIPADNIYLDIHYMDAYKIF
                     TWHPQRFPHPDQLVNNLKKMGFHTTAIIDPGIKVEKGYTAYEDGLKQHMFIKYPDGEP
                     YQGQVWPGWCHFTDYTKPVARQWWGKQFASLVELGIDGFWNDMNEIATWGSATPTFIQ
                     FDWEGYKTTYRQAKNIYGMLMARSTYEGTKELMHGGRPFVLTRSGYAGMQRYTSIWTG
                     DNQANDNHMLLGIRLVNSIGLSGVAFTGYDIGGFGGDASPALYARWISLGALSPFCRT
                     HSAYNTKSSEPWSYGEDVEDIARNYINLRYRLLPYLYSQFYEASQTGMPVQRALAIEY
                     AQDPRSLHTSYQNQYSLGPSLLVAPCKSTDEYVKVFLPRGNWFNIYNDEFLNGDNEII
                     VESPLQRLPVFVKAGSIIPVHPVRQHTAEDPGDTLELHLFYEKGLHEFIYYEDDGLTY
                     NYKNGEFYKRIIRFNGDRRELTLSSKEGSLPSHFHSLKIVLHGFEKLDSNFQKNITYQ
                     KISVLPDGFGYDIKNPCITLAFDENEIKIKL"
BASE COUNT          628 a          470 c          477 g          664 t
ORIGIN      
        1 caccttcttt ggctaattcg agggcaatgg ctttaccaat acccgatgat gcacctgtaa
       61 ttattgcaac cttagaatcg agtctcatca tttacttaaa gttttatttt aatttcgttt
      121 tcatcgaaag caagtgtaat gcatggattt ttgatatcgt agccaaatcc atcgggtaag
      181 actgaaattt tttgataagt aatatttttt tgaaagttgg agtcgagttt ttcgaatccg
      241 tgcaaaacaa ttttgagact atgaaaatga gaaggcaacg atccctcttt ggacgatagg
      301 gttaattctc ttctatcgcc attaaaacgg ataatgcgtt tgtaaaattc accattttta
      361 taattgtagg ttaatccatc gtcttcgtaa taaataaatt catgaagtcc tttttcgtag
      421 aaaagatgga gttcgagcgt atctccggga tcctcggccg tatgctgacg tacaggatgt
      481 actggaatta tagagcccgc cttcacaaat acgggtagcc gctgaagagg cgattctaca
      541 atgatttcat tatcaccgtt taaaaattca tcattataaa tattgaacca gttgccacga
      601 ggcagaaata cctttacata ttcgtcggta cttttgcaag gagctaccaa aagagagggt
      661 ccaagagaat attgattctg gtatgaggta tgcaaggagc gaggatcttg tgcatattca
      721 atagccagtg ctctttgaac tggcataccg gtttgtgatg cttcatagaa ctggctatac
      781 agatacggca agagtcggta gcgcaagttg atataatttc tggcaatatc ttctacatct
      841 tctccatagc tccaaggttc ggaagatttg gtattatagg cgctgtgagt tctgcaaaag
      901 ggagagagag ctcctaagct gatccatctg gcataaagtg ctggcgaggc atcacctcca
      961 aacccgccaa tatcgtatcc cgtaaaagca acgccagata atccaatgct gtttaccaac
     1021 ctgattccga gtaacatgtg attgtcgtta gcttggttat cgcctgtcca aatggaggta
     1081 tagcgttgca ttccggcata acccgagcga gtgagaacaa agggccttcc cccatgcatc
     1141 aattccttgg ttccttcgta agtactgcgt gccattagca ttccataaat gttttttgcc
     1201 tgacgataag tggttttata tccttcccaa tcaaactgta taaaagtcgg ggtggccgaa
     1261 ccccatgtag caatctcgtt catgtcgttc cagaatccat ctatacccaa ttcgacaagg
     1321 ctggcaaact gtttacccca ccattgacgg gctactggtt tggtataatc ggtaaagtgg
     1381 caccatcccg gccaaacctg cccctgataa ggctcaccat ctggatactt gataaacatg
     1441 tgctgtttca aaccgtcttc ataagcagta tatccttttt ctactttaat tcctgggtct
     1501 ataatcgcgg tagtatgaaa tcccatcttt ttcaaattat tgaccaactg atccggatga
     1561 gggaaacgct gaggatgcca tgtaaatatc ttataggcat ccatgtagtg aatatcgaga
     1621 tagatattgt cggcagggat ttttttatca cgaaacatag atgcaatttc gagtacttcg
     1681 gtatcggggt agtaactcca gcggcattgc tgattgccaa gggcccagat aggaggaagc
     1741 tccatacggc cggtaagcca tgtataattc ttgatgattt cggctacatt ttctccattg
     1801 atgaaatagt aatccatttc tccatcatcg gccccaaaag aggagaacct ttcttgcgaa
     1861 gctccaaaat taaaatagga tttataggtg ttgtcaaaga aaataccgta ggtcaaaccc
     1921 gaatgcagcc caatgtaaaa aggtatagtg gaatataagg gatcggaata ttcgtcataa
     1981 cgagggttat cagtattcca atgccaatag gcgttcccgc ggcggtcgaa gtttcctgtc
     2041 ttctcgccta aaccaataaa atgttcccct gcttgcagaa ttttacaggt ggttacctcg
     2101 gtgccattcc agtttgtccc taatgcaggg tcatccccgt tgatgattcg accatccagg
     2161 gtcagaaatg taaatcttac agggttcttg ccgattttca atatagtagt cgaagttttc
     2221 agaatgatgt tttcggcat
//
LOCUS       DAORPD010000216         2237 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_8822_length_2237_cov_2.999542, whole genome shotgun sequence.
ACCESSION   DAORPD010000216 DAORPD010000000
VERSION     DAORPD010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8822_length_2237_cov_2.999542"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1072
                     /locus_tag="PK976_03390"
     CDS             <1..1072
                     /locus_tag="PK976_03390"
                     /inference="COORDINATES: protein motif:HMM:NF020939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RQC domain-containing protein"
                     /protein_id="HRC66963.1"
                     /translation="TICRRKVLLHYFGEQYTQDNCGNCDNCLHPKTRYDGKEAVLTVL
                     DAITQTKQQCKSKHLVHVIIGKLTANVKAAKHHKLEVFGRGEEYDEPYWHSVVRQMLV
                     ENLIEKDIENYGILRITNEGKKFLKKPKTIMLTQDREYDNNEDDELIDQNTGAKPGTA
                     DATLFALLKDLRKEISRKENLPPFVIFQDPSLEEMAIQYPITLDELKQITGVGTGKAS
                     KFGKPFIDLIKNYVEENEIIRPNDMVIKSVINKSGLKIYIIQSIDRKVPLEDIALAKN
                     LSFDELLTEIEHIVASGTKIDISYYINEYIDEYHQEEVFEYFRTAETDSIEKAREELG
                     EEEFSEEEIRLMRIKFMSEMGN"
     gene            1264..1884
                     /locus_tag="PK976_03395"
     CDS             1264..1884
                     /locus_tag="PK976_03395"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HRC66964.1"
                     /translation="MKNKFNLSLILSLLSLFGVIVLFILYFTHRPLGREGIPKAAETG
                     GHRIAFFRTDSILHHYDFVKERAEELAQKSRKYGEDLHRRQSEFENEAAYFQESVRKN
                     ALSEKSAQEIYQELMQKQQSIIDLKDRYDMELAGEEAQINLILFDSVTNLLKRFNKSY
                     GYDYILGYNKNGNVFYTNDTFDITQMVIKDLNREYRAVKGKTSGSK"
     gene            1899..>2237
                     /locus_tag="PK976_03400"
     CDS             1899..>2237
                     /locus_tag="PK976_03400"
                     /inference="COORDINATES: protein motif:HMM:NF025494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4296 domain-containing protein"
                     /protein_id="HRC66965.1"
                     /translation="MKKDKCLFLMITVLLFIGCIQPKEKKPGALITPRKMAEILTDLQ
                     IADAWIAIKQNEGQDIKLLSTQIEDSLFKKHHITRAQFDTSMVWYSAHAEELDKIYDQ
                     VIQQLELLKEQ"
BASE COUNT          785 a          447 c          416 g          589 t
ORIGIN      
        1 cacgatctgc cgacgaaaag tacttcttca ttactttgga gaacaataca cacaagataa
       61 ctgtggcaac tgcgacaatt gtctgcatcc taaaacacgt tacgacggaa aagaagccgt
      121 tctaaccgtg ttggatgcta taacacaaac caaacaacaa tgcaaatcga aacatctggt
      181 gcatgtaatt ataggaaaac ttactgcaaa tgtcaaagca gccaaacatc acaaactcga
      241 ggttttcggt agaggtgaag aatatgatga accttactgg catagcgttg taagacaaat
      301 gctggtagaa aacctcatcg agaaagacat agaaaattat ggcattctta gaattacaaa
      361 tgaaggcaaa aagtttctca aaaaaccaaa aacgattatg cttactcagg accgcgaata
      421 cgacaacaac gaagacgacg aactaatcga tcaaaataca ggcgctaaac ctggcacagc
      481 agatgcaaca ctctttgccc tcttaaaaga tttgcgcaaa gaaatatcga gaaaagaaaa
      541 cctgcctcct ttcgtcatct ttcaagaccc tagcctggaa gaaatggcta ttcaatatcc
      601 tattactctg gatgaactga aacaaattac cggagtagga accggcaaag cctcgaaatt
      661 tggaaaaccc ttcatcgatc tgattaaaaa ctatgtcgaa gaaaacgaaa ttatccgccc
      721 taacgatatg gttatcaaat cagtaatcaa taaatcgggt ttgaaaattt atatcattca
      781 gagcatcgac cgtaaagtcc ccttagaaga cattgcttta gccaaaaatc tttctttcga
      841 cgagctgctt accgaaatcg aacacattgt tgcttcggga accaagatag acataagtta
      901 ttatatcaac gaatatatcg atgaatacca tcaagaagag gtttttgaat atttccgcac
      961 agccgaaacc gattctatcg aaaaggcacg cgaagaactt ggagaagaag aattttcgga
     1021 agaagaaatt cgcctaatgc gtatcaaatt tatgtcggaa atggggaatt aaacgatatt
     1081 catttcgaaa atgcaagggt tgctttatga ttaactttgg ccaatctcat agcaaaccca
     1141 gctgtgttta tttgttcgat caatcactct tcataaaatt tctgtcccct atatgaactt
     1201 caacatcaat aatacgagca ttctttaact ttgctagtca aacctttctt tattcttata
     1261 attatgaaga acaaattcaa tcttagcctt attctcagcc ttttatcgct atttggcgta
     1321 attgtcctgt ttattctcta ttttacacat cggccattgg gtagagaagg cataccaaaa
     1381 gctgccgaaa cgggaggcca tcgcattgct tttttccgaa ccgattccat tctccatcat
     1441 tatgatttcg taaaggaaag agctgaagaa cttgcgcaaa aatcacgaaa atatggggaa
     1501 gaccttcatc gaagacaatc ggaattcgaa aatgaagccg cttattttca agaaagtgtc
     1561 agaaaaaatg ccctttccga aaaaagtgct caggaaattt atcaagaact aatgcaaaag
     1621 cagcagagca taatcgatct gaaagatcgc tatgatatgg agctggctgg cgaagaagca
     1681 caaatcaacc tcatcttgtt cgactcggta accaatttgc tgaaacgttt caacaaaagc
     1741 tatggttatg attatatcct tggctacaat aaaaatggaa atgttttcta caccaacgac
     1801 acattcgata ttactcagat ggttattaaa gatttgaaca gagaatacag ggccgttaag
     1861 ggtaaaacat ctgggtctaa ataagaataa agcattttat gaaaaaagat aaatgcttat
     1921 ttctaatgat aacggtttta ttgttcattg gctgcattca gcccaaagag aaaaaacctg
     1981 gtgctctcat cacgccccga aagatggctg agattctcac cgatttgcag attgcagatg
     2041 cctggattgc cataaaacaa aacgagggtc aggatattaa attgctttct actcaaatcg
     2101 aagattcgct ttttaaaaaa caccatatta cccgggctca gttcgatacg agtatggttt
     2161 ggtattcggc acatgcagaa gagctcgaca aaatttacga ccaggtaatc cagcagcttg
     2221 aattgttgaa agagcag
//
LOCUS       DAORPD010000217         2236 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_8829_length_2236_cov_2.884915, whole genome shotgun sequence.
ACCESSION   DAORPD010000217 DAORPD010000000
VERSION     DAORPD010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8829_length_2236_cov_2.884915"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(153..2003)
                     /locus_tag="PK976_03405"
     CDS             complement(153..2003)
                     /locus_tag="PK976_03405"
                     /inference="COORDINATES: protein motif:HMM:NF015210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HRC66966.1"
                     /translation="MPKEITIQIQGKERTVSELLKDRKYSIDYYQREYRWETKHVTEL
                     IEDLSNKFFSYYDKEHELTQVGNYGNYFLGSIVISHKEGENFIVDGQQRLTSLTLLLI
                     YINNLQKSRTNHVNISDLIFSEVYGVKSFNIDIDERKNCMEFLYNDRKEQIDLTEAPE
                     SVRNLVNRYTDIEENFPDELKGEALPYFIWWLQKKVVLVEIIAYSDEDAYTIFETMND
                     RGLSLSPTDMLKGYLLANITGSENKDSLNELWKNQIYDLIKISKEDETDFFKVWLRAK
                     YAETIRERKKGATNKDFEIIGTAFHKWVRDEHIKIGLNKPKDYRDFIQEQFIYFSNYY
                     LDIRKASIQFKREFEYIYYNASNYFTLQNPLLLAPLTVEDDPETVRKKIRLVSGYIDI
                     FIARRVWNFRTLGYSSIVYTMFNLMKEIRGKEIPELVNLLHQKVDEMEDTFDTNDRLR
                     IHQQNHYYIHRMLARITNYIDTQCGIESSYATYIDPNIKKPFEVEHIWADRFERHTDE
                     FSHPTDFGDYRNRIGGLILLPKGFNQSLNDDDYETKVEAYFGQNLLAKSLNQSCYEKN
                     PSFLQFIQHSGLSFKPYSHFNKADLDERQELYRQICKKIWSPSRFDDELN"
BASE COUNT          686 a          417 c          360 g          773 t
ORIGIN      
        1 tggttttgcc cgattgcggc aagccgataa ttcctaactg catgctgtgc tcctgagtgg
       61 gtttgtgtaa ctatgtgaat tatactcggt tagcgaggat gaaacagcct aatagaccga
      121 gtgacagatt tcatttaaaa ggctggcttt tttcaattta attcatcatc aaaacgactt
      181 ggtgaccaaa tttttttaca tatctgacga tatagttctt ggcgttcatc taaatctgct
      241 ttattgaaat gagaataagg tttgaaagat aagccactat gttgaatgaa ctgcaaaaat
      301 gacggatttt tttcatagca gctttgatta agcgattttg ctaggagatt ttgtccaaaa
      361 taagcctcta cttttgtctc ataatcatcg tcattcaagc tctgattgaa ccctttagga
      421 aggaggatca atcccccaat tcgattacgg taatcaccaa aatctgtggg atgggaaaat
      481 tcatcggtat gcctttcaaa tctatcagcc catatatgct cgacttcaaa aggtttcttg
      541 atattgggat caatatatgt agcataacta gattcaatcc cacattgtgt atcaatataa
      601 tttgtaatgc gggctaacat acggtgaata taataatgat tctgttgatg aatcctaagg
      661 cgatcgttcg tatcgaaagt gtcttccatt tcatcaactt tttggtgaag caagtttact
      721 aattcgggga tttccttgcc tcgaatttct ttcatcaaat taaacatagt atatacaatg
      781 gaagaatatc caagagttcg gaaattccat acccgtcgag ctataaatat atctatatag
      841 cctgaaacaa gccttatttt ctttctcaca gtttcagggt catcttcaac ggtcaaaggc
      901 gctagcaaca atggattttg taaagtaaag taattgctcg cattgtagta aatatattca
      961 aattctcttt taaattggat cgacgctttt ctaatatcca ggtaataatt ggagaaatat
     1021 atgaactgtt cttgaataaa atcacgataa tctttaggtt tatttagacc aattttaata
     1081 tgctcgtctc ttacccattt gtgaaaagca gtaccaatta tctcaaaatc tttattcgtg
     1141 gctccttttt ttcgctcacg aatcgtttca gcatacttgg cccgtaacca aaccttaaaa
     1201 aaatcagttt catcttcttt actaattttt atcaaatcat atatctgatt tttccaaagt
     1261 tcattcaaac tgtctttatt ctctgaacca gtaatatttg caagtaggta accctttaac
     1321 atatctgtag ggcttagaga taacccccga tcattcatgg tttcaaaaat agtgtaggca
     1381 tcttcatctg aataagcgat gatttctacc aacactacct tcttttgtaa ccaccaaata
     1441 aaataaggca atgcttctcc tttgagttca tcgggaaagt tttcttcaat atctgtatag
     1501 cggttgacta agttacgaac tgattctggg gcctcagtta aatctatctg ctctttacga
     1561 tcgttataca aaaattccat gcaattttta cgttcgtcta tatcaatatt aaatgatttc
     1621 actccataaa cttctgaaaa gattagatct gaaatattga catgattagt gcgagatttt
     1681 tgtagattgt ttatgtaaat caataaaagt gtcagagatg ttagacgctg ctgaccatca
     1741 actataaaat tttccccttc tttatggcta ataacaatgg aaccaagaaa gtaattccca
     1801 taattaccca cctgggtcaa ttcatgttcc ttgtcataat acgaaaaaaa tttgttagaa
     1861 agatcttcaa ttaactctgt aacatgctta gtctcccaac gatattctcg ttgataatag
     1921 tcaatggaat atttcctatc ttttagtaat tcactgacag ttcgctcttt tccttgaatt
     1981 tgtatagtaa tttctttagg cattttccca ttcctttgag cttatccaac gaatatacat
     2041 tataacgaaa atctaaacga cttgctaata aggaatccag atattcacaa tattctgatt
     2101 accttttaat acatcttttt ctagaggcat aacattattt aatgaatgaa aatttgccat
     2161 ttgcattttc cttgacccgc atgcaggctt tggtatactt aatttgccag ccgaagtggc
     2221 ggaataggca gacgcg
//
LOCUS       DAORPD010000218         2227 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_8889_length_2227_cov_2.205341, whole genome shotgun sequence.
ACCESSION   DAORPD010000218 DAORPD010000000
VERSION     DAORPD010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8889_length_2227_cov_2.205341"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="PK976_03410"
     CDS             complement(<1..756)
                     /locus_tag="PK976_03410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRC66967.1"
                     /translation="MTTNKKSALGRGLSAILQSPETDITSRDISGEFVAGAIAEIEID
                     KIEANPFQPRSEFEEASLNELAESIHQQGIIQPITVRKMGYDKYQLITGERRLRASKM
                     AGLEKIPAYIRIANDEEMLEMALVENIHRDDLNPLEIAMSYQRLIEEVGIPVNDVASK
                     VGQNRSTVVNYTRLLKLPPEVQIAIRDVQISMGHARAIINVEDIEDQLIILKEIIDHD
                     LSVRQVEQWVRDLKKAQVSQETEIKKLPNEKPVL"
     gene            complement(746..1561)
                     /locus_tag="PK976_03415"
     CDS             complement(746..1561)
                     /locus_tag="PK976_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008773873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRC66968.1"
                     /translation="MGKIIAIANQKGGVGKTTTAINLGASLAVLEFKTLLVDADPQAN
                     ATSGLGFDPRNITSSIYECIIGDIDAAKIILTTETPNLDLIPAHIDLVGAEIEMINLP
                     NREKMMRKVLSPIKDSYDFIFIDCSPSLGLITVNALVAADSVIIPVQCEYFALEGLGK
                     LLNTIKIVQSRLNTNLDIEGILLTMYDGRLRLARQVVEEVKMHFQTMVFDTIINRNTR
                     LSEAPSFGQSIIMFDADCVGTANHLNLAREILQKNNLTKHSPLNQNNLNFHDN"
     gene            complement(1691..2023)
                     /locus_tag="PK976_03420"
     CDS             complement(1691..2023)
                     /locus_tag="PK976_03420"
                     /inference="COORDINATES: protein motif:HMM:NF023109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HRC66969.1"
                     /translation="MMNEREKTNKGYLDLFSRTIIARNRRYYFDVKSGENNLVLTLTE
                     SKRKFNNRNGKFYYERNRVIVFSEDMDNFMNSFREVEKFLKDYLKRHPDISNFPSPPN
                     AFGSKDSP"
BASE COUNT          604 a          453 c          468 g          702 t
ORIGIN      
        1 caggacaggt ttttcgttgg gtaatttttt aatttccgtt tcctgactta cctgggcttt
       61 tttcaaatcg cgcacccatt gttcaacctg gcggacagaa aggtcgtgat cgataatttc
      121 tttcagaatt atcaattgat cttcgatatc ttcgacgttg atgattgccc gagcgtgtcc
      181 catgctgatt tggacatcgc gaatggctat ttgaacctcg ggaggaagtt ttaacaggcg
      241 ggtataatta acgaccgtgg agcggttttg acctaccttg ctggccacat cattgacagg
      301 aattcccact tcttcgatga gtcgctgata gctcatggct atttccagtg ggttcaggtc
      361 gtcgcgatga atattttcca cgagtgccat ttcgagcatt tcctcgtcat tggcaatacg
      421 gatgtaagca ggtatctttt cgaggccagc catcttagaa gctctaagcc tgcgctcgcc
      481 tgtaatcaat tgatacttat cgtatcccat ttttctgacg gtaatgggtt gaatgatgcc
      541 ctgctggtga atggattcgg ccaattcgtt caaagaggct tcttcgaatt cagatcttgg
      601 ttgaaaagga ttagcttcga ttttatcaat ttcgatttcg gctatggctc ctgccacaaa
      661 ttctcccgaa atatctctcg aagtaatatc ggtttcggga ctctgcaata ttgcgctcag
      721 ccctcgcccc aaggccgatt tcttattagt tgtcatgaaa attcaagtta ttttgattta
      781 atggggaatg tttggtaagg ttatttttct gcagaatttc tctggccaga ttcagatggt
      841 ttgcagttcc aacgcaatcg gcatcaaaca tgatgattga ctggccgaaa ctgggagctt
      901 cgctcaagcg tgtatttcta ttgatgatgg tgtcgaagac catggtttga aagtgcatct
      961 ttacttcttc gacaacctgc cgagcaagcc tcaaacggcc atcatacatg gtaagcaata
     1021 tcccttcaat gtcgagattg gtgttaaggc gcgattgaac gatttttatg gtattcagaa
     1081 gctttccgag gccctcgagg gcaaaatatt cgcattgaac cgggataatg accgaatcgg
     1141 cagctaccaa tgcattcacc gtaatcaaac ccagcgaagg agaacaatcg ataaagataa
     1201 agtcataaga atctttaatg ggagacagaa ctttccgcat cattttttct ctgttgggca
     1261 ggttgatcat ttcgatttct gcacccacca gatcgatatg tgcagggatg agatcgagat
     1321 tgggggtttc ggttgtcaaa atgatcttag ccgcatcgat atcgccaata atgcattcgt
     1381 atatgctaga agtaatatta cgaggatcga aaccaaggcc agaggtggca tttgcctgag
     1441 ggtcagcatc taccaataaa gttttgaatt ccaatacggc caggctggct cccagattga
     1501 tcgccgtcgt tgttttgcct acaccacctt tttggttcgc aattgcgata attttaccca
     1561 taaaaatata ttcgagaaat ggaactttcc atcagcaaaa ttaaacaaat atatgtctgc
     1621 tttacctaaa attaggctat cctatcggct gattcaatgt aacaaactga ttgataattt
     1681 atttacttgt tcagggggaa tcttttgagc cgaaagcatt cggtggagaa gggaaattcg
     1741 aaatgtcagg atgtcgtttg agataatcct tcagaaattt ctccacttcc cgaaaagaat
     1801 tcatgaaatt atccatatct tcggaaaata cgataaccct attgcgctca taataaaact
     1861 tgccattgcg gttgttgaac ttgcgtttgc tctcggtcaa agtcaaaaca agattgtttt
     1921 cgccggattt tacatcgaag taatatcgtc tattccttgc tatgatggtt cttgaaaata
     1981 aatctaagta acctttgttt gttttttctc tttcattcat cattttaatt acttatagtt
     2041 ttaatgagac aaaaatagtg ggatgaatcc cgacatgcaa aattttccat taaaaattta
     2101 gattaagatt tagaaacaga aattccgagg ttctcgattg ttgtgagcca cttgataatg
     2161 atgtattttt gcagccgtaa aaaaatgctc cttctcaaac atgcttacac gacgtcatct
     2221 tagaatc
//
LOCUS       DAORPD010000219         2213 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_8964_length_2213_cov_3.298424, whole genome shotgun sequence.
ACCESSION   DAORPD010000219 DAORPD010000000
VERSION     DAORPD010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8964_length_2213_cov_3.298424"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(372..444)
                     /locus_tag="PK976_03425"
     tRNA            complement(372..444)
                     /locus_tag="PK976_03425"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(409..411),aa:Gln,seq:ctg)
     gene            complement(588..1319)
                     /locus_tag="PK976_03430"
     CDS             complement(588..1319)
                     /locus_tag="PK976_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HRC66970.1"
                     /translation="MENWLERTELLLGKEKLEALKRSHVLVAGLGGVGAYLAESLCRA
                     GLGELTLADSDIVKPSNRNRQLIALSSTEGRLKAEAMAERLRDINSEIHLHIITQYLK
                     DDVLKDVVCRPYDYVADALDTLSPKLFLLIHSVNYGHKIISSMGAGSKIHPERIMVAD
                     ISETNTCPLALLLRKRLKKHGISKGIKAVFSYEAVMEGSRKYVNDELNKKTIVGTISY
                     MPAIFGLFMASVIIRDLTGTLPVPY"
     gene            complement(1303..1965)
                     /locus_tag="PK976_03435"
     CDS             complement(1303..1965)
                     /locus_tag="PK976_03435"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRC66971.1"
                     /translation="MLCFDVHTHHFRADNRSIFNLILKEDGSMIPEMYFSSGIHPWYI
                     TDKWKKILEDHFRENYKQRVAIGETGIDMLKGTDLKLQELVFMAHIEWALNYSMPLVI
                     HSVRSSQRIIEILRAMNFQFPVVIHGFRQSNELAKKFLSEGYYLSLGVYVNYAGQKLT
                     EAIKYIPLDRILIESDDFDGDCCEIYHQIAHIKGISADELDNVVLMNVKNCFRIIDGK
                     LA"
BASE COUNT          726 a          459 c          350 g          678 t
ORIGIN      
        1 acactcattg tgttcagctc ttttttaatt tctctagtct gctctcaaat aatatttcat
       61 caaacctttt ggggaaaacc caagtgaaca gcccaaaaaa tagcgattgc acaattctat
      121 gacatataca agttatagaa atggaaatgc aattacttag agttggttat aaacccttgc
      181 ataaagttta ttattaaacg aagaaaactc aacctgcctt acccattcgt gaaagattag
      241 agtttttgca ctaatgcttt aataggcatc aaggaagaaa tgaaagattt caccaacaaa
      301 actgctgcga aagattatta caaacaaaaa aaggacttgg ttttttgagc cagtcctttt
      361 ctctattgtt gttgtcctgc cagggatcga acctggagtc ttctgatcca gagtcagacg
      421 tgttgccaat tacactacag gacaggatta tctttcattt gacagtgcaa aattataaat
      481 aaatcatcat agagcaacta cttttatagt cttgggcaag aattcttgcc gcctaaatca
      541 tcttcataaa tgatccatct attcgaaaga ttatacattc tcataattca ataaggaaca
      601 ggcaacgttc cagttaaatc gcggatgatc acagaggcca tgaacaagcc aaaaatagcc
      661 ggcatgtatg atatagtacc tacaatagtt tttttattga gctcatcatt cacatatttt
      721 ctacttcctt ccattacagc ttcataagag aaaacggctt ttattccttt cgaaatccca
      781 tgctttttga gccttttcct taataataag gctaatggac atgtattagt ctccgaaata
      841 tcggcaacca taattctttc ggggtgaatt ttactgccgg ctcccatcga gctaattatt
      901 ttatggccat aatttaccga atgtatcagc aggaaaagtt ttggggaaag tgtgtccaaa
      961 gcatctgcaa cgtaatcata aggtcgacac acaacatctt tcaatacatc atcctttaga
     1021 tattgagtta ttatatggag atgtatctca gaattgatat cacgcaatcg ctcagccatg
     1081 gcttcagcct ttaacctccc ttccgtactt gatagggcga ttagttgccg attacgattg
     1141 gaaggtttaa caatatcaga atcggcaaga gtcaattctc ccaaaccagc tcgacataag
     1201 ctttcggcca aataggctcc tacacctccc agtccggcca ccaaaacatg cgacctcttc
     1261 aaagcctcga gtttctcctt ccctaataga agttctgtac gttcaagcca attttccatc
     1321 gattatccga aaacagttct tcacattcat taagactaca ttgtccaatt catcggcact
     1381 tattcctttt atatgagcta tttggtggta aatttcacaa caatctccat caaaatcatc
     1441 actttcgatc aatattctat ctaaagggat atattttatc gcttctgtca acttctgtcc
     1501 tgcataattt acataaacac ccagcgaaag ataatacccc tcggatagaa attttttggc
     1561 aagctcgtta ctttgtctaa atccatgaat aacaacagga aattggaaat tcattgcacg
     1621 aagtatctcg atgattcgtt gcgaagaacg aacggaatga attaccaaag gcatgctata
     1681 attcagagcc cattctatat gagccataaa aactaattcc tgaagtttta aatcggttcc
     1741 cttgagcata tcgatgcccg tctccccaat agccactctt tgcttataat tttcccgaaa
     1801 atgatcttca agaatcttct tccacttgtc agtaatatac cagggatgga ttccactcga
     1861 aaaatacatt tctggaatca tcgaaccatc ttctttcaat atcagattaa atatggagcg
     1921 attatcagcg cggaaatgat gtgtatggac atcgaaacat aacataatta atcagctatc
     1981 gaactgcaaa tattagaaga aatattttat ttggtaaagt taaatgaatg atattattgc
     2041 aaactataga taccttgacg attcgaagga ggaatccaac caaattaatc ggaaataata
     2101 taaatcgctg atttaaaata atttatatta aaaattctct tctcgattgg taaaaaattg
     2161 ctttatccaa aatcatcgat ctgaaaaacg ttcgacgaaa aaagccctgc aat
//
LOCUS       DAORPD010000220         2212 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_8969_length_2212_cov_2.654149, whole genome shotgun sequence.
ACCESSION   DAORPD010000220 DAORPD010000000
VERSION     DAORPD010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8969_length_2212_cov_2.654149"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..918)
                     /locus_tag="PK976_03440"
     CDS             complement(<1..918)
                     /locus_tag="PK976_03440"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRC66972.1"
                     /translation="MENNIYIEVSCQQIHHEGEKICGDVFLSRKIKEEGRTIAVLSDG
                     MGHGVKANVLATLTATMALNFTIEHKDVDKIAELIMNTLPVCSERKISYSTFSIVDIQ
                     IDDLTKILEYDNPQCIILRGNDPFQPNWNCVILKSEKNAGKELMTCQFYHHKEDRIVL
                     ISDGVAQSGMGSEKYPFGWGRDNVQDFVVQLLTNDPQISAATLASKVVNFAHINDGYR
                     SKDDTSCAVIYYREPRKLLICTGPPFDEHKDREYAEAVKTFEGKKILCGATTADIIAR
                     ELGVEISDSFEFEDPDLPPISYMPGVDLVT"
     gene            complement(937..>2212)
                     /locus_tag="PK976_03445"
     CDS             complement(937..>2212)
                     /locus_tag="PK976_03445"
                     /inference="COORDINATES: protein motif:HMM:NF024586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HRC66973.1"
                     /translation="NPIDEILTFIELRKMFKEAGITEQTLEYSEFDSPIGHLGSLFPI
                     SNGLLQAVGLDENLLTGTITTIEGKDNFIDSVRQFHDYTELIRRHFNIFYCHGCLMGP
                     GTSPGGEKYLRRSLAVEYANKRLKEFDSQSWQENIEKYKTITLSRSFNPDDQRLQSPP
                     KEKIDEVLKVIGRVDADKLMGCGACGFSSCYEFATAVASGLAKPEMCITYNLRNQNEY
                     IKTLKATNEKLAKTEIALKESEKIARREQMLAREANEIVNIMLQKLPSGVVIVDENLK
                     IIQANKTFIETLGEDARLIDEVIPGLVGADLKTLLPYHFYNMFSYVLKNAEDITNRDV
                     NFNDNILNVSIFTIRPNKIVGAVVRDLKMPEVRREQIINRINEAITENLEMVQKIGFL
                     LGEGASKTERMLNSIISAYQSENGNKSGETKS"
BASE COUNT          565 a          471 c          455 g          721 t
ORIGIN      
        1 ggtaaccaga tcgactccag gcatgtaact tataggaggc aagtcggggt cttcaaattc
       61 gaaagagtcg gaaatttcga ctcccagttc acgagcaatg atatcggccg tagtagctcc
      121 gcacagaatt tttttccctt cgaaggtttt tactgcctcg gcatattctc tgtctttatg
      181 ctcgtcgaat ggaggccctg tgcatatgag aagttttcgt ggttctctat aatagatcac
      241 agcacagctt gtatcgtctt tactgcgata gccgtcatta atgtgggcaa agttgacgac
      301 tttggaagcc agtgtggcag ctgaaatttg cgggtcatta gtaagaagct gaacaacaaa
      361 atcctgcaca ttgtctcttc cccaaccaaa gggatatttt tctgacccca tgcccgattg
      421 agccacccca tcacttatta gaacaatacg atcttccttg tggtgataaa attgacaggt
      481 catcaattct ttacctgcat ttttttcact cttaagaatt acgcaattcc agttgggctg
      541 aaaagggtca ttccccctta aaataataca ttgaggatta tcatattcga ggattttggt
      601 taaatcatca atttgaatat ctacgatgga aaaggtactg taactgattt tacgttcact
      661 gcaaacaggc aaggtgttca tgattagctc agctattttg tccacatcct tgtgttcgat
      721 ggtgaagttc aatgccatgg tggcagtaag agtagccaga acattggcct tcacgccatg
      781 ccccatgcca tcggaaagaa cggcaatggt gcggccttct tccttgattt ttctcgatag
      841 gaaaacatct ccgcagattt tttctccctc gtgatgaatc tgctggcaac ttacttcgat
      901 gtatatatta ttctccataa atgaatcatc ttcatattag gattttgttt ctccactttt
      961 attgccgttt tccgattgat atgcgctgat gatggaatta agcatccttt cggtcttact
     1021 ggcaccttct cccaacaaaa aaccaatttt ttgaaccatt tcgagatttt cggtaatggc
     1081 ttcattaatc cggttgataa tctgttcacg ccttacttcg ggcattttta gatcgcggac
     1141 gacagcccct actattttgt tcggccttat cgtaaatatt gaaacattga gtatattgtc
     1201 gttaaaattg acatcccgat tggtaatatc ttcagcattt ttcaagacgt acgaaaacat
     1261 attgtaaaaa tgatagggta acaaagtttt taaatctgct ccaaccaagc caggaattac
     1321 ttcatcaatc aggcgagcgt cttctcctaa agtttctatg aatgtcttat tcgcttgaat
     1381 gatttttaga ttttcatcga cgatgaccac gcccgaaggg agtttttgta acatgatatt
     1441 tacaatctcg ttggcttcgc gcgctaacat ttgttcgcgg cgggcaatct tttccgattc
     1501 tttcagagca atttcggttt tggcaagttt ttcgttggtg gctttcagtg ttttaatata
     1561 ttcgttttgg ttgcgaagat tgtacgtaat gcacatttca ggtttagcca atcccgaggc
     1621 taccgcagtg gcaaattcat agcaagacga aaacccgcat gctccgcatc ccatcaattt
     1681 atcggcatct actcgtccta tcactttcag tacttcgtcg atcttttctt tgggtggaga
     1741 ttgaagcctt tggtcatcag ggttaaaact gcgggaaagt gtgattgttt tgtatttttc
     1801 gatgttctcc tgccatgatt gggaatcgaa ctctttgagt cttttattgg catattctac
     1861 agcgagtgaa cgcctgaggt atttttctcc tcctgggctg gtgccagggc ccattaggca
     1921 gccatgacaa taaaatatat tgaaatgcct tcggatcaat tccgtgtaat cgtggaattg
     1981 tctgaccgaa tcaatgaaat tatccttgcc ttcgatagta gtaattgtcc ctgtgagtaa
     2041 gttttcatcg aggccgaccg cttgaagtaa tccattgctg atgggaaaca gggaaccaag
     2101 gtgtccaata gggctatcga attctgaata ctccagcgtt tgttcagtga tacccgcttc
     2161 cttgaacatt tttcgcaatt caataaaagt gagaatttcg tctatgggat tc
//
LOCUS       DAORPD010000221         2210 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_8982_length_2210_cov_2.731787, whole genome shotgun sequence.
ACCESSION   DAORPD010000221 DAORPD010000000
VERSION     DAORPD010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8982_length_2210_cov_2.731787"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..1135)
                     /locus_tag="PK976_03450"
     CDS             complement(20..1135)
                     /locus_tag="PK976_03450"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRC66974.1"
                     /translation="MADKQPIKVLHVSTQTGWRGGEQQIMYLVRLGKEVRHYLFCPEE
                     AALRKEAKKEEITIKTFDSSSKFSFFLKVPFQLWQFISEVRPHLIHVHDSGAHTLAYL
                     LARRKELALPLIVSRRVDIRRGKNIFSRKKYNQPEIAKIFCVSNYVKTIMESVVKDRN
                     KLLTIYSGIDLQRFLYRSPYPKLRNELDIPDDYLIVGNIAALTKPKGYPVFVKTAAEL
                     LKHRQNFIFVIAGDGPMRANIEKMIQNLGLEKNVILLGFRDDIPEILPSLDILLFPSE
                     NEGLGTTILDAMACGVNVVATSAGGIPEMITHQMNGLLAPVGDIQQLASNVLRLACNA
                     ELRNMLKENAYITVKNFRVENMVTQTIKAYHEIPGTI"
     gene            complement(1114..1875)
                     /locus_tag="PK976_03455"
     CDS             complement(1114..1875)
                     /locus_tag="PK976_03455"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRC66975.1"
                     /translation="MEALSVVIITYNEERNIERCIRSVVGIADEIVVVDSFSTDRTRE
                     IAGEFGAKVIKHEFEGYVQQKNYALAQATHRWVLSLDADEALSEKLRNSIKEIKFNPS
                     ADGYTMNRLTNYCGKWIRHSGWYPDKKLRMVLKKKAYWTGTNPHDKLELFESKNIRHI
                     PGDILHYSYYSLEDHLQQIDRFMQIRAYELFEKRIKPNVWHFYLKPLHKFFKHYFFQL
                     GFMDGYEGYQIARLSAFGIFIRYAKLRELWRTNNP"
BASE COUNT          680 a          449 c          381 g          700 t
ORIGIN      
        1 cattcgaata aaataaaatc taaatagttc caggtatttc atgataagcc tttatggttt
       61 gagtcaccat attttcgacc ctgaaatttt tcaccgtgat gtaagcattt tctttgagca
      121 tatttcgcaa ttcagcattg caagccagac gtaatacatt agaagccagc tgttgtatat
      181 caccaacagg tgccagtaat ccattcattt gatgggtaat catttcgggg atgccacctg
      241 ccgatgtagc tactacattc accccacatg ccatagcgtc cagaatggtt gttcccagcc
      301 cctcgttttc ggatgggaag agcaaaatat ccagagaagg caaaatctca ggtatatcgt
      361 ccctaaatcc taacagaata acatttttct ccaatcccaa gttttggatc attttttcga
      421 tgttcgctcg cataggacca tcgccagcaa tcacaaatat aaaattctgc cgatgtttca
      481 ataattctgc cgcagttttt acaaaaaccg gatatccttt aggcttcgtt aaagcggcaa
      541 tattaccaac aataagataa tcgtcgggaa tgtctaattc attgcgtaat ttgggataag
      601 ggctccgata gagaaaacgc tgtaaatcga tgccactata aatggtcagt aatttatttc
      661 tgtctttcac caccgattcc ataatcgttt tcacataatt actgacgcag aaaatcttgg
      721 caatttcggg ctgattatat tttttacgtg agaagatatt ttttcctcga cgaatatcga
      781 ctctacggct cacgatcaaa ggtaaagcca gttcttttct tctggccaat aaataggcaa
      841 gtgtatgagc tccactatcg tgaacgtgaa tgagatgagg ccgaacttca ctgataaatt
      901 gccataactg gaatggaacc ttcaagaaga agctaaattt cgatgaagaa tcgaaagttt
      961 ttatcgtaat ttcttctttt tttgcttctt ttctcaaagc ggcttcttca gggcaaaaca
     1021 agtaatgcct aacttctttg cccaatctga cgagatacat gatttgttgt tcacctccac
     1081 gccaacctgt ttgcgtcgaa acatgcaaaa cctttatggg ttgtttgtcc gccataattc
     1141 acgaagttta gcataacgaa taaagattcc aaatgccgat aatcgagcaa tttgatagcc
     1201 ttcataacca tccataaacc cgagttgaaa gaaataatgt ttgaaaaatt tatgtaaggg
     1261 tttgagatag aaatgccaaa cattgggttt gattcgtttt tcaaaaagct cataagcccg
     1321 aatttgcata aaacgatcaa tttgctgaag atgatcttct aaagaataat aggaatagtg
     1381 cagaatgtcg cctggaatat ggcgaatatt tttactttca aaaagttcga gtttgtcgtg
     1441 tggattggtg cctgtccagt aggctttttt tttcaaaacc atcctcagtt ttttatctgg
     1501 ataccagcca ctatggcgaa tccattttcc acaatagttg gttagtcgat tcatggtata
     1561 tccatcggcc gaaggattaa atttaatttc ctttatacta ttccttagct tttcagaaag
     1621 agcttcgtcg gcatcgagag aaagaaccca acgatgtgtg gcttgggcaa gtgcatagtt
     1681 cttttgctgt acataacctt cgaactcatg cttgatgacc ttcgcgccga attcacctgc
     1741 aatttctctg gttcgatcgg tcgaaaatga atctacaaca actatctcgt cagcaattcc
     1801 aactaccgac cgtatacagc gttcgatatt cctttcttca ttataagtga tgatgacaac
     1861 cgataaggct tccattcgtc ttaacaattt ctgagacaaa caattgtaaa aaaatgaatc
     1921 gtaaagaaaa ttcttacaaa tattgcgata aatttacaac tcacataaaa agtagttcat
     1981 tcatcaaaaa ttaatcttct gccaaacatc ttcatttcgt taaattccgc atctatacat
     2041 gaaactgatt ttcgaatcca tcatttttac tcaaatctgt ttaaaaattg tatatttgcg
     2101 ccttcatcag atatttcaat cccctatttt gaaagggata agaattcaat caaaatcgat
     2161 acattaggca aaatatttct ttgcctctta taatgaagtg aacatggttt
//
LOCUS       DAORPD010000222         2208 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_8999_length_2208_cov_2.267998, whole genome shotgun sequence.
ACCESSION   DAORPD010000222 DAORPD010000000
VERSION     DAORPD010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8999_length_2208_cov_2.267998"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..1460)
                     /locus_tag="PK976_03460"
     CDS             complement(123..1460)
                     /locus_tag="PK976_03460"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRC66976.1"
                     /translation="MSKISLIIEREYITRVKKKTFIIMTILGPILIAALWIVPIYVTT
                     LSQGKKTVEILDETGLFFDQFQSDEHFRFVPIFTDLETAKKGLIASGDDALVYIPRTE
                     LNVPVTAILYSTSQTGIDIKTVVKENLRKQVEALKLKAKNIDPEVLTSIKTSIIINSI
                     KIGKDGSEVKSSEEISIALGLLSGIIIYMLIFMFSSQVMRGVIEEKANRIVEVIVTSV
                     KPFQLMMGKIIGVGLVGITQFVLWIVFTGVIIFGFQITHPAIQQNVSQIENFSSTSIV
                     SSGQSVDLNEDMVLESNIITQALEGIRAINIPLLLFAFILFFLGGYLLYSAMFAAIAA
                     AVDNEADTQQFVLPITIPLLITIILSGFIINNPQSQLAVWLSIIPFTSPVSMMMRIPF
                     GVPVWQLILSILLLVATFIIATWIAGKIYHTGILLYGKKITWADLIKWISMKL"
     gene            complement(1470..>2208)
                     /locus_tag="PK976_03465"
     CDS             complement(1470..>2208)
                     /locus_tag="PK976_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRC66977.1"
                     /translation="QPNHTDKIGYLPEERGLYKKMKASEQAIYLARLKGVSQHEAVSR
                     LKYWFRKFEILSWWDRKVEELSKGMQQKIQFIVTILHQPELLIFDEPFSGFDPINVDL
                     IKNEILKLRDEGASIIFSTHSMESVEQLCDNIALIHQSKVVLSGAVNEIKSRFSQNLF
                     EVESYKADLSTITPNLPQGIHYQKTEPNSSLYKHYFKLDASLAPNDLLASLLPYMQIT
                     GFRELLPSMHDIFVSTIQNGSLPANPS"
BASE COUNT          676 a          418 c          441 g          673 t
ORIGIN      
        1 tctgcattct ttgagtagat ttatagttaa tgtttttttc gaaggggcaa aattagaaga
       61 ttatttcatt cctcccaatt ttaaattatt caaaaagatg atgtatagag ttgaattgtg
      121 ttttatagtt tcatagaaat ccatttgatc agatcggccc aggttatttt ctttccatag
      181 agcaatattc cggtatgata gattttcccg gcaatccagg tggcaatgat aaaagtagcc
      241 acaagtaaaa gaatagacag aatcaattgc cagactggaa ctccaaatgg aattctcatc
      301 atcatactaa cgggtgaggt aaaagggata atagagagcc atactgccag ctggctttga
      361 ggattattga tgataaaccc cgatagaata atggtaatta acaacggaat ggttatagga
      421 agcacaaact gctgagtatc tgcttcgtta tctacagctg ctgcaatagc ggcaaacatg
      481 gccgaataaa gaagataacc tcccagaaag aatagaatga aggcaaatag tagcaaggga
      541 atattaattg ctcgaatccc ttcgagtgct tgggtgataa tattcgattc cagcaccata
      601 tcttcattca aatcaaccga ttgaccagaa gacactatac ttgtgctgga aaaattttct
      661 atttgtgaaa cattttgttg gatagcagga tgagttatct gaaatccaaa aatgataacc
      721 ccagtaaaca ctatccatag aacgaattga gtaatgccca ccagccctac gccaatgatc
      781 ttacccatca tcaattgaaa aggcttaacc gaagtaacga tgacttcgac aatacgattg
      841 gctttttctt cgatcacacc acgcatcact tgcgaactaa acataaaaat caacatatag
      901 atgatgattc ctgaaagtag tcccaacgca atgctgattt cctccgacga tttgacctct
      961 gaaccgtctt tgccaatctt tatcgagtta atgatgatgc tcgtcttgat agaagtcaat
     1021 acttcagggt cgatattttt ggccttcagt tttaaagctt cgacctgttt acgcagattt
     1081 tcctttacca cggttttgat gtctataccg gtttgggagg tagaatataa aatagccgtt
     1141 acgggtacat tcagctctgt acgagggata taaaccaatg catcgtcgcc cgaagctatc
     1201 aatccttttt tagcggtttc cagatcggtg aagataggaa caaatcgaaa atgctcgtcg
     1261 gattgaaatt gatcgaaaaa caatcccgtt tcatcaagaa tctcgacggt ttttttgccc
     1321 tggctcaaag tggttacata aataggaact atccagagtg cagctattaa aataggtccc
     1381 agaatcgtca tgatgataaa cgtctttttt ttgactctgg taatgtattc tcgttcgatg
     1441 atcagggata ttttactcat actcgaaaat taggatgggt ttgccggtaa agagccattt
     1501 tgaatggtag aaacaaagat atcgtgcata gaaggaagga gttcgcggaa tccggtaatc
     1561 tgcatataag gcaaaagaga agccaaaaga tcatttggag ctaaagaagc atccaatttg
     1621 aaataatgct tgtataagct ggaattgggt tcggtttttt gataatgaat tccttgcggc
     1681 aaattcggtg ttatagtgct taggtcagct ttgtaagatt ctacttcgaa cagattctgg
     1741 ctgaagcgac ttttaatttc gtttactgca ccgctaagca ccactttcga ctgatgaatc
     1801 aatgcaatat tgtcgcatag ttgttcgacc gattccatac tgtgagtcga aaaaatgata
     1861 cttgctcctt catctcttaa tttcaaaatt tcgttcttta tcaaatccac attgatagga
     1921 tcgaatccgc taaagggctc atcgaagatc aataattcag gttgatgaag tatagttacg
     1981 atgaattgga ttttttgctg catacccttg gaaagttctt ctacttttct atcccaccag
     2041 ctcaggattt cgaatttcct gaaccagtat tttagacggg atacggcttc atgttgtgaa
     2101 actcccttga ggcgagccag ataaatagct tgttcgctag ctttcatctt cttatacaat
     2161 cctctttctt cggggagata accgatttta tcggtatgat taggttgc
//
LOCUS       DAORPD010000223         2208 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_9000_length_2208_cov_2.100790, whole genome shotgun sequence.
ACCESSION   DAORPD010000223 DAORPD010000000
VERSION     DAORPD010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9000_length_2208_cov_2.100790"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2208
                     /locus_tag="PK976_03470"
     CDS             <1..>2208
                     /locus_tag="PK976_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005867246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRC66978.1"
                     /translation="VGRVLFNQVIPKEIGFINQMLTKKNLRDIIGEVLKRTGTAVTAK
                     FLDDIKEMGFMTAFKAGLSFNMGDVIIPDNKDELIAEANAEVEEVIGNYTMGFITNNE
                     RYNQIIDIWTHTNSKLTLTLMDKLTKDKQGFNSLFMMLDSGARGSKEQIRQLAGMRGL
                     MAKPQKSSGSGGEIIENPILSNFKEGLSVLEYFISTHGARKGLADTALKTADAGYLTR
                     RLVDVAHDVIINEEDCGTLRGLTVTALKKDEEIVESLYDRILGRVALHDIYHPYTNEL
                     IATAGQELTEEICEIIEKSPIESVDIRSVLTCESKSGVCALCYGRNLAKGKMVQKGET
                     VGVIAAQSIGEPGTQLTLRTFHVGGVAGNIALTSRIEAKYDGRLQIDELRSVEYTSES
                     GRTYHVVLGRSAEMHIVDPNTEIILTSAHIPYGANLYFKSSSMVRKGDLICDWDPYNA
                     LIISDVPGVVAYHNLIEGVTYRTESVEQTGHPYKVVVESRQKTKNPAIRILNADGEEI
                     KIFDIPVGAHLSVEDGQKIKAGDILVKIPRAVGKSGDITGGLPRVTELFEARNPSDPA
                     RVSEIDGIVTFGKKLKRGNRELIVTSRVGEEKTYLIPTTKQILIQEHDYVKAGQPLSE
                     GAISPSDILAIKGPMAVQEYIVNEIQEVYRLQGVKINDKHFEVIVRQMMRKVEIEDPG
                     DSKFLEGELVNKPDFQEENDWLFGKKVVIDPGDSPNLSAGQIISPRKLRDENSM"
BASE COUNT          653 a          438 c          528 g          589 t
ORIGIN      
        1 gttggaagag ttttattcaa tcaggttatt cctaaagaga tcggttttat taatcaaatg
       61 ctgaccaaga agaacctgag agatatcatt ggcgaagtat tgaaacgtac gggtactgcg
      121 gtaactgcta aatttctcga tgatatcaag gaaatgggtt tcatgacggc cttcaaggcc
      181 gggctttcgt tcaatatggg tgatgttatc atacccgata ataaagacga attgatagcc
      241 gaagccaatg ccgaagtcga agaagtaata ggcaactata cgatgggctt tattaccaat
      301 aatgagcgct ataatcagat catcgacatt tggacgcata ccaattcgaa acttacgctt
      361 acattgatgg ataagctcac caaagataag caaggattca attccttgtt catgatgctg
      421 gattcaggtg ctcgtggttc gaaggaacag atcaggcagt tggctggcat gcgtggtttg
      481 atggcaaaac ctcagaaatc gagtggtagc ggtggtgaaa tcatcgaaaa tcccattcta
      541 tcgaacttca aagaaggctt gtcggttctg gaatatttca tttctacaca tggtgccaga
      601 aaaggattgg ccgatactgc cctgaaaact gctgacgctg gttatttgac taggcgcctg
      661 gttgatgttg cccatgatgt gattatcaat gaagaagatt gtgggacctt aagaggttta
      721 accgtaacgg cactcaagaa agacgaagaa attgtcgaga gcctttacga ccgtatctta
      781 ggtcgcgtag cccttcatga tatttatcat ccatatacca acgaattgat tgctactgcg
      841 ggtcaggaat taaccgaaga aatctgcgag attatcgaaa aatcgcctat tgaatctgtc
      901 gatatacggt cggttcttac ttgtgaatcg aaatccggcg tttgcgcctt atgttatgga
      961 cgaaatctgg ccaaaggaaa aatggttcag aaaggtgaaa ccgttggagt aatcgctgcc
     1021 caatctatag gtgaacctgg aactcagctt acccttagaa ccttccacgt tgggggagtt
     1081 gctgggaata ttgccttgac ctcacgcatt gaggcaaaat atgatggtcg cctccagata
     1141 gatgaattgc gctcggttga atatacttcc gaaagcggcc gtacttacca tgtcgttctt
     1201 ggtcgttcgg ccgaaatgca tatcgttgac cccaacaccg agattatcct tacttcggca
     1261 catattccct atggagccaa cctgtatttc aagagcagct ccatggtgag gaaaggagac
     1321 ctgatctgcg attgggatcc ctataatgcc ttgataattt ctgatgttcc cggtgtagtt
     1381 gcttatcata atttgataga aggagttaca tacaggactg aatcggtcga acaaaccggc
     1441 catccttata aggtagtggt cgaaagccgt caaaagacca aaaacccagc catacgtatt
     1501 ttgaatgccg atggagagga aataaaaatt ttcgatattc ctgtcggagc tcatctttcg
     1561 gttgaagatg gacagaaaat caaagctgga gatattttag taaaaatacc tcgtgctgtg
     1621 ggtaaatcag gtgatattac cggaggttta ccgcgtgtta ccgaattatt cgaagctcgc
     1681 aatccgtccg atcctgcccg tgtttctgaa attgacggta tagttacctt tggcaaaaaa
     1741 ttgaaacgtg gcaaccgtga actcatcgta acgagccgtg tgggcgaaga aaagacttat
     1801 ttgattccta ccaccaaaca gattctaatt caggaacacg attatgtaaa ggccggccaa
     1861 cccctttcag aaggggcaat ctcgccaagc gatattcttg ctattaaagg tccaatggct
     1921 gttcaggaat atatcgtcaa cgaaattcaa gaagtttatc gcttacaggg cgtaaaaatt
     1981 aacgataagc attttgaagt aattgttcgg cagatgatgc gtaaagttga gatcgaagac
     2041 ccgggcgatt cgaaattcct tgagggtgaa ctggtcaata aacctgattt tcaggaagag
     2101 aacgactggt tatttggcaa gaaggtggta atcgatccgg gagattcgcc aaatctttcg
     2161 gcggggcaaa tcattagtcc acgcaaactg cgagatgaaa attcaatg
//
LOCUS       DAORPD010000224         2208 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_9002_length_2208_cov_1.978170, whole genome shotgun sequence.
ACCESSION   DAORPD010000224 DAORPD010000000
VERSION     DAORPD010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9002_length_2208_cov_1.978170"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(284..490)
                     /locus_tag="PK976_03475"
     CDS             complement(284..490)
                     /locus_tag="PK976_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66979.1"
                     /translation="MDNQLQRCNIRFSNYVFNQENNVSLRKFIMAIIGGERDPDKLCS
                     LVHGCTLNKYGRDVIQASLDGVIQ"
     gene            1096..1545
                     /locus_tag="PK976_03480"
     CDS             1096..1545
                     /locus_tag="PK976_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5606 domain-containing protein"
                     /protein_id="HRC66980.1"
                     /translation="MELKDIVAISGKTGLFKVVGQTKTGAIVESLIDGKRQPVFIHDR
                     LSSLADITVFTTEEDRPLRKILQAIRERENGGPAPSHKSDETTLKNYFATVVPDYDNE
                     RVYFSAIKKIISWYNLLHEKGLLDLLNEPEEKDISEENSTPVENPEQ"
     gene            1628..>2208
                     /locus_tag="PK976_03485"
     CDS             1628..>2208
                     /locus_tag="PK976_03485"
                     /inference="COORDINATES: protein motif:HMM:NF013161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRC66981.1"
                     /translation="MPGKFIHDFIVLRNISLNASHFILELLCPIQLPEIFPGQFVEVK
                     VEHSPATFLRRPFSIHEVDRQENCLQLLIQIKGDGTRQLSLLEPGESLNMIYPLGNSF
                     HMVKHEKVLLVGGGCGVAPLLFLARILKENENELYILIGAKTKEDILEKKKFEKFGKV
                     FITTEDGSEGRLGLVIHHGLWRQTPFMADKIYT"
BASE COUNT          703 a          452 c          421 g          632 t
ORIGIN      
        1 agatcgcatt cgcagcatga attggaaatt tgatttcgac cctacacaga aaaatttcgg
       61 attcaaggca aaaattcttt atatcatcga aaaaatcttc ggctggagac cattcgaata
      121 taaaaattat aggctgctga aataaaaaaa tgctacacag caaagggatt gcctcggcaa
      181 aatatgcatt tgccctttct tctaaatagt taaggcaagt agcctgctga ctatccaata
      241 aagatattta ttcattacac tgtcgaaaca tttcaacgtc aacttattgt attacgccgt
      301 ccaatgaggc ttgaatgaca tcgcgcccat atttgttcaa agtgcaaccg tgaacaagtg
      361 aacaaagttt gtccggatcc cgttcgccac ctatgatggc catgataaac ttacgcagcg
      421 agacgttgtt ctcctgattg aaaacataat tactgaaacg aatgttgcag cgttgcaatt
      481 gattgtccat ctgccggacc agatgtgccc ggcctttgtt cagccgccgg catcggcgtg
      541 tgtattgcca catcggatgc aataaatcac cagccacaaa actgtccctt atttggttct
      601 tttatagaca ttcgcctatc cactctgcct ctttggtatc cgatttacga cttggcaatt
      661 gattgatgaa gtatggctta gcccacttta actcaaaatc aaatttcatt acgtgccata
      721 ctaacatcca atagatcctc gtgccttcta ttgctaccca cccgcatcca ctttccataa
      781 gcttatcgcg aagggacatc aaatcggtag ttgatacctc gaaaaattgt tgaaattttt
      841 tttgcttttt ctgtacaaaa cacattcaaa tgcactatct ttggggatgt caaggccgca
      901 tgcttttcca taaatttgtc agatttaaat taataacagg gccgtgaagc ccgttaatta
      961 aaacaccggt aacaattgtg cttcgagcat gtacgacaag ccgttggaat ttgcacggtc
     1021 ttgattctta tttcagcgca aggaattcag cgaaatttca tgtaattttg caagcggaat
     1081 cttaaatttt ggcaaatgga attaaaagat attgtagcaa tttcaggaaa aacgggtctt
     1141 tttaaagtag taggccaaac taaaacgggt gccattgtag aatccttgat cgacggtaaa
     1201 agacaacccg tatttattca cgaccgtttg agcagtctcg ctgatattac tgtttttacc
     1261 accgaagaag accgtccttt gagaaaaatc ctacaggcca tcagggaaag ggaaaatgga
     1321 gggccagccc caagccataa atcagatgaa accaccctaa aaaattattt cgcaaccgta
     1381 gtacctgatt acgacaacga acgagtctat ttttcggcga taaaaaaaat catctcatgg
     1441 tacaaccttc ttcacgaaaa aggattactc gatttattaa atgaacccga agaaaaagat
     1501 atttctgaag aaaactcaac accagttgaa aatccagaac aataatcttc caaaaatccg
     1561 tcgatacaaa aaagacggat ttttttatgt tattttgtaa tcgaagttcc gttcatgaaa
     1621 atttattatg ccaggcaaat tcatccatga ttttatcgtt ctaaggaata taagcctgaa
     1681 tgcatcgcac ttcatccttg aattgctttg tcctatacaa ctacctgaaa tttttccagg
     1741 gcagttcgta gaagtaaaag tggaacattc accggctaca tttctaaggc gtccatttag
     1801 cattcatgaa gtcgataggc aagaaaactg ccttcaactt ctcatacaaa ttaaaggtga
     1861 tggaactcgt caactttcct tactcgagcc aggcgaaagt ttgaatatga tttatccatt
     1921 aggaaattct tttcacatgg taaaacatga aaaagtgcta ttggttggag gaggatgcgg
     1981 tgtagctccc ttgttgtttc ttgcaaggat tttaaaggaa aacgaaaacg agctctacat
     2041 tttaattggg gctaaaacga aagaggatat ccttgaaaag aaaaaattcg aaaagtttgg
     2101 taaggttttc atcaccaccg aagatgggag tgaaggaagg cttggtcttg tgattcatca
     2161 cggcctttgg aggcaaacgc cgtttatggc agacaaaata tatacctg
//
LOCUS       DAORPD010000225         2205 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_9019_length_2205_cov_2.629767, whole genome shotgun sequence.
ACCESSION   DAORPD010000225 DAORPD010000000
VERSION     DAORPD010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9019_length_2205_cov_2.629767"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /gene="pyrF"
                     /locus_tag="PK976_03490"
     CDS             <1..693
                     /gene="pyrF"
                     /locus_tag="PK976_03490"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007174182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HRC66982.1"
                     /translation="IDATLPFAIAYKPNFAFYESRGLVGWMSLKRTMDYLESFREEIF
                     TIADAKRGDIGNTSQQYAKAFFDKQSSGFDFDAATINPYMGTDSVSPFLEFPDKWVVL
                     LTLTSNKGAVDFQHLQKEGSKERLFEIVIKKSLEWGSTPSNMMYVVGATQASLLLKIR
                     KILPEHFFLVPGIGAQGGNLEEVLKYGSIREGGLLINASRSVIYASDGTDFSIRAAEV
                     ACALQCQMAAFI"
     gene            complement(1059..>2205)
                     /gene="yidC"
                     /locus_tag="PK976_03495"
     CDS             complement(1059..>2205)
                     /gene="yidC"
                     /locus_tag="PK976_03495"
                     /inference="COORDINATES: protein motif:HMM:NF002356.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HRC66983.1"
                     /translation="YSTDKEVKNLNEAKDDKKIITTSLKWVSLKSKFFLATLIAENAF
                     PNGAEISTYIDPERANEPRYNKSMSVQIPLEYGFSERMDYPMQIYFGPNQYKILHAYN
                     LRLERQIPLGWGFFLMWPVNVYAIIPVFNWLSNYGVNYGIIILILTVLLKIVLFPITF
                     VSYRSTAKMRALKPEIDEINKKYQRKEDAVKKQQATMDLYKKAGVNPMSGCLPMLLQF
                     PILIAMFRFFPSSIELRQQPFLWAHDLSSYDSILDLPFKIPFYGDHVSLFTLLMTIAT
                     LFYTKINNDLMNTGQQQIPGMKLMTYLMPILFLGIFNNYSAGLSYYLLVAQLITFGQM
                     FIFRKAINEEKLRARLLANKNKPVKKSNFQKRLEEMARQQQQQARRK"
BASE COUNT          643 a          416 c          462 g          684 t
ORIGIN      
        1 atcgatgcga cactgccttt tgccatagcc tacaagccca attttgcctt ttacgaaagc
       61 cgcggactgg ttggctggat gagtctgaaa agaaccatgg attatctgga atcttttaga
      121 gaagaaattt ttacaatagc ggatgccaaa cggggcgata ttggaaacac aagtcagcag
      181 tatgccaaag catttttcga caaacaaagt tccggcttcg atttcgatgc agctacgatt
      241 aatccttaca tgggtaccga ttcggtttcg ccttttttgg aatttcctga caaatgggtt
      301 gttttattaa cgctaaccag caataaggga gcagttgatt ttcaacatct tcaaaaagaa
      361 ggttccaaag agcggctctt cgaaattgtt ataaaaaaat cattggaatg gggttcaacg
      421 ccttcgaata tgatgtacgt cgttggtgca actcaagctt ctttactttt gaaaattaga
      481 aaaattttac cggaacactt ttttttagtt cctggaattg gtgctcaagg aggaaatttg
      541 gaagaagttt tgaaatatgg atcgatccgt gagggcggcc ttttgataaa tgcctctcgc
      601 agtgttattt atgctagcga tggaacagat ttttcgattc gtgctgccga agtagcttgc
      661 gcacttcagt gtcaaatggc ggctttcatt tgatgaatct ttgaaaaaga gtggtttgta
      721 ttacgcttct ttggagacta attgatcggc agacaaaagg acgaggagaa taagagattt
      781 ggagacgagg agactaaaag aatacccatg tcgaatatca tgaattattt tttaaatgat
      841 aacatatcca ttttcagtct tttaattaat acaccaatcc ctatccccca aacccttcca
      901 ccatttctcc ctttctgctt tatagatttt caacttttta ctcaccaatc cccctccaaa
      961 tgccattctc cgttcatcgt ctttatttat gctgtaactt ctcaaacatg tcgcacatcg
     1021 cgaaacggag gatcgcccct gcttgtcatt tatgtaaatt atttccttct tgcctgttgt
     1081 tgttgctggc gagccatctc ttcgaggcgt ttctgaaaat tcgacttttt aaccggttta
     1141 ttcttattgg ccagcagtct tgctctcaat ttttcttcat tgatagcttt gcggaagata
     1201 aacatctgtc caaaggtgat aagttgtgcc acaagtaaat aataactcaa accggcggag
     1261 taattattga aaatgccgag gaataaaata ggcattaaat aggtcataag tttcatgccc
     1321 ggtatttgtt gttgcccggt attcattaag tcgttattga tttttgtata gaagagtgtt
     1381 gcaatagtca tcagtaatgt aaagaggctg acatggtcgc catagaaagg tatcttgaaa
     1441 ggcaggtcga ggatagaatc ataactggaa aggtcgtgag cccagagaaa gggttgttgc
     1501 ctgagctcga tggaagaagg gaaaaacctg aacatggcaa taagaatagg gaattgtaac
     1561 agcatgggca aacagcccga catggggttt actccggcct ttttatacaa atccatagtt
     1621 gcctgctgtt ttttcacggc atcttccttg cgttgatatt tcttgttgat ttcatcgatt
     1681 tctggcttca acgcacgcat tttcgccgta gaacggtaag aaacaaaagt gatgggaaat
     1741 aagactatct tcagcaaaac ggttaaaata aggataataa tcccgtagtt tacaccgtaa
     1801 ttgctcaacc agttaaatac aggaataatt gcataaacat ttacaggcca cataagaaag
     1861 aagccccatc ctaatggaat ttgcctttct aatcgtaaat tataggcatg taaaatttta
     1921 tattggttgg ggccaaagta aatctgcatc ggataatcca tcctctcaga aaagccgtat
     1981 tctaaaggaa tttgaacgga cattgatttg ttatatctag gttcgttagc ccgctcggga
     2041 tcgatatagg tagaaatctc ggcaccatta gggaatgcat tttcagcaat gagtgtggca
     2101 agaaaaaatt tcgatttcaa tgaaacccat ttcaagctag tagtaatgat ttttttatcg
     2161 tctttggctt cattgaggtt ttttacctcc ttgtcggtgg aatag
//
LOCUS       DAORPD010000226         2204 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_9026_length_2204_cov_2.124244, whole genome shotgun sequence.
ACCESSION   DAORPD010000226 DAORPD010000000
VERSION     DAORPD010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9026_length_2204_cov_2.124244"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            161..820
                     /locus_tag="PK976_03500"
     CDS             161..820
                     /locus_tag="PK976_03500"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRC66984.1"
                     /translation="MASNRKKSYIEKLIEKGENQHLDFKFAVNDSRKIARSMVAFANT
                     DGGRLLIGVKDNGVIAGVKTVEEFHMVETAAHIFSRPPIPFRSIEWNMGGKMILEIII
                     SKGRHRPYYAQDEHGQWTAWLRQSDRDIKAPKILTEYWTRKSSGEITTLSYGPFEKFF
                     LQMLEKQGLVSLESFRRLMGISRNQANEIALQFMLMDIIAIRFENDKPFFFLKQGSTL
                     F"
     gene            complement(806..1045)
                     /locus_tag="PK976_03505"
     CDS             complement(806..1045)
                     /locus_tag="PK976_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66985.1"
                     /translation="MSAYYQNKDELIEILGEKIAYLNKVLFHNTSSEFYLEDIIEAID
                     FLKDHKYVLTGQGLNQLEFYIHEAEESLRRYLKKS"
     gene            1222..>2204
                     /locus_tag="PK976_03510"
     CDS             1222..>2204
                     /locus_tag="PK976_03510"
                     /inference="COORDINATES: protein motif:HMM:NF036933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgGFc-binding protein"
                     /protein_id="HRC66986.1"
                     /translation="MRNFLLLLAGLILTINGFSQSDTEFWFAGPENESNNGLRDRPLY
                     IRVATFNLPATVTLSQPARPQFTPITVDINANSTYTFDLQYPTDFTDVIETKPQNQVL
                     NYGLKLTSTNPITAYYEQSSSSNPDIFTLKGRNALGTRFIVPFQTTYYNSQRTDLGGT
                     KFSWFVVVATEDNTHVTIKPTVAIEGHVANVPFTVTLNKGQTYACRSIGQSPAEHAAG
                     TLITSDKPIAVTTNDDSVCQCQSCTNIGPDLIGDQIISYNVFGKEHIIVKGYFTVSGS
                     SQSADRIYVFADSANTALYINGTYVSTINNPGEFYTYNFGDENVIYLSASKP"
BASE COUNT          718 a          440 c          394 g          652 t
ORIGIN      
        1 gctatgaacc agggccgttt gtgtgataac cccatccaaa tcgaaaataa cagccgaaaa
       61 aggatagttt ttaagcataa tttgtacgat tcccggcaca aaattatgat atttgcaaga
      121 acttaatgcc ctaaacccga cgcttaaagc ctgataagcc atggcctcca atagaaagaa
      181 atcttacatt gaaaaattaa tcgagaaggg cgagaatcaa cacctcgatt ttaaattcgc
      241 cgtaaatgat tcccgtaaaa ttgcccgttc tatggtagcc tttgctaata ccgacggagg
      301 ccggttattg attggagtga aagacaatgg agttattgca ggagtgaaaa cagtcgaaga
      361 gtttcacatg gtagaaacgg ctgcacatat tttttcacgg cctccgattc catttagaag
      421 tattgaatgg aatatgggag gaaaaatgat tttggaaatt attatttcga aaggccgaca
      481 tcgcccgtat tatgctcaag atgaacatgg acaatggact gcatggcttc gacaaagtga
      541 ccgggacatt aaggcaccaa aaatattgac agaatattgg actcgtaaat cttcgggaga
      601 aattactacc ttatcttatg gtccatttga aaagttcttc cttcaaatgc ttgaaaaaca
      661 agggttggtt agtttggaaa gttttaggcg attaatggga atcagccgaa atcaagccaa
      721 cgaaatcgct ctacagttta tgttaatgga tattatcgct atcagattcg aaaacgataa
      781 accctttttc ttcctaaaac agggttcaac tcttttttaa atacctccgc aatgattctt
      841 cagcctcatg aatgtaaaat tccagctgat ttaaaccttg cccggtaagg acatatttat
      901 gatctttcaa aaaatcaatg gcctctatga tatcttccaa ataaaattcg gaagaagtat
      961 tgtgaaacag caccttattc agataggcaa ttttttcacc caaaatctct atgagttcat
     1021 ccttattctg gtaataagcg ctcattcttt catttatgaa gtggcaaata tatacaattc
     1081 aaacctaact gaacttatca aatcaaacct cacaggtctt ggagacctgt gaggtttctt
     1141 cgtatttatt taacctatat ctttgtggtt ctataactaa ataacatttc atggtgtcat
     1201 aaaaacattg aaaagaatca aatgaggaat ttccttctct tattagctgg attaatatta
     1261 acgataaatg gcttttcgca atcagatacc gaattttggt ttgcagggcc agaaaatgaa
     1321 tccaacaatg gattacgtga taggccatta tacattcgag tagctacttt taatttacca
     1381 gctacagtta ccctaagtca gcccgctcgc ccccaattta ctccaattac cgtagatatc
     1441 aatgcaaata gtacttatac ctttgattta caatatccta cggactttac cgatgtaatc
     1501 gaaacaaaac cacaaaatca agttctaaac tatggtttaa aacttaccag tactaatcct
     1561 ataactgcct attacgagca aagcagcagc agcaatccag atatttttac cctgaaagga
     1621 agaaatgcct taggaacaag gttcatagtt ccattccaga caacttatta caacagtcaa
     1681 aggactgatt taggaggcac aaagttttca tggtttgtag ttgtagctac cgaagacaac
     1741 acccatgtaa ctataaaacc cacggtagcc atcgaagggc atgtggccaa tgttccattc
     1801 acagtaacct tgaataaggg tcaaacctat gcttgccgtt ctataggaca aagccctgcg
     1861 gaacatgcag ctggaacgct cattacttct gacaaaccaa tcgctgtaac cacaaatgat
     1921 gattctgttt gtcagtgtca atcctgcacg aatattggtc ccgacttaat tggtgatcaa
     1981 atcatttctt ataatgtatt tggtaaggaa cacatcattg tgaaaggtta ttttaccgta
     2041 tccggttctt ctcaatcggc cgaccgaata tatgtttttg ccgattcggc caatacagct
     2101 ttatatatca atggaactta tgtatcgacg attaataatc caggggaatt ttatacttat
     2161 aattttggcg atgaaaatgt aatttatctt tccgcttcca aacc
//
LOCUS       DAORPD010000227         2199 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_9057_length_2199_cov_1.704757, whole genome shotgun sequence.
ACCESSION   DAORPD010000227 DAORPD010000000
VERSION     DAORPD010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9057_length_2199_cov_1.704757"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /locus_tag="PK976_03515"
     CDS             <1..811
                     /locus_tag="PK976_03515"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HRC66987.1"
                     /translation="AANVNIEVSCNRIRVWFQQNTDSRYRIITQGFIGQDMQGNMVTL
                     GREGSDYSAALFATALSANSVVLWKDVPGVMNADPDIYAQAVPFKFVSYREAVELTYY
                     GAKIIHPKTIKPLENAGIPLYVRSFLHPSEQGTLIGPGNGTIPQIPSIIHKANQILIT
                     ISHKDLSFITEKTLLTVLSVFDNSSLKINLMQNSALTFSVCLDDVKGKIDQILTRLME
                     SFNVKYNRKLVLLTFRYYAQQPRFIDQWIEGRSVLVEQRSRNTAQYVIAEK"
     gene            833..>2199
                     /locus_tag="PK976_03520"
     CDS             833..>2199
                     /locus_tag="PK976_03520"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HRC66988.1"
                     /translation="MKKYFFFSFLTFLIFYFPFVSQSQHRQQISLDKSWKFHLGSASS
                     AEKDFGYGLVNIYSKNGKSEGTAIYPGFDDSNWRRVNLPHDWAIELPFINHPSFDVMS
                     HGYKPVGGFFPETSIGWYRRSFDISRSDSGYRFSLTFDGVYRDAKFWLNGYYLGNNQS
                     GYSGVEFDITDFVWFDKPNTIVVRVDATQYEGWFYEGAGIYRHVWLNRYPEIHIPSDG
                     VFAYSIPKGKSCIIHFETTISNSSLNDKMVSMETKVKSRDGRLIAQNMSKSVRTASSG
                     QTRVNQQIKIDNPHLWNLDDPYLYRIETSILSEGKILDKVSMPFGIRFITWDADKGFF
                     LNNKPVKLLGVNMHQGHAGLGVALPNGMQYYRINRLKQMGANAYRCSHHPPTLELLDA
                     CDSLGMLVINENRLLNTSHEYLNQFSRMVIRDRNHPSVILWSIGNEEGYIQTNGNGKR
                     MAITL"
BASE COUNT          652 a          461 c          434 g          652 t
ORIGIN      
        1 cgctgccaat gttaatatcg aagtttcatg caatcgaatc agagtatggt ttcagcaaaa
       61 taccgattca cgatatagaa taattactca aggttttata ggccaagata tgcagggcaa
      121 catggttacg cttgggcgcg aaggttccga ttattctgct gccttgtttg ccacggcact
      181 ttcagccaat agtgttgtgc tttggaaaga tgtcccaggc gtgatgaatg cagaccccga
      241 tatttatgct caggccgtgc cttttaaatt cgtatcctac cgcgaagccg tcgaactcac
      301 ttattatgga gctaagataa tacatcccaa aaccatcaaa cctttggaaa acgcaggaat
      361 cccattgtat gtccgatctt ttctccatcc gtcagaacaa ggcacactca ttggaccggg
      421 aaacggaact attccacaaa tcccctctat tatccataaa gccaaccaaa ttttaattac
      481 tatttctcat aaagaccttt ccttcatcac agaaaaaacc cttttgacag ttttatctgt
      541 ttttgataat tcctccttga agatcaattt gatgcaaaat tctgctctca ctttttcggt
      601 atgtttggat gatgtaaagg ggaaaatcga ccaaatcctt actcgactca tggaatcttt
      661 caacgtaaaa tataatcgta agcttgtcct gttgactttc cgttattatg ctcagcaacc
      721 tcgatttatc gaccaatgga tagaaggaag gtccgttttg gtggaacaac gcagccgcaa
      781 cactgcccaa tatgttattg ctgaaaaatg atgtcctaat aataattaag ccatgaaaaa
      841 atattttttc ttttcgtttc tgacatttct aattttctat tttccatttg tatcacaaag
      901 ccagcataga cagcaaattt ctctggataa aagctggaaa tttcatttgg ggagtgcttc
      961 atctgccgaa aaagatttcg gttatggatt ggtaaatatc tattcaaaaa atggcaaatc
     1021 ggaaggtacg gccatttatc ccggttttga tgacagtaac tggcgtaggg taaatttgcc
     1081 tcacgactgg gctatcgaac tccctttcat aaatcatccc tctttcgatg taatgagtca
     1141 tggatataaa cctgttggcg gatttttccc tgaaacctct ataggatggt acaggcgaag
     1201 ttttgatatt tcacgttccg attcaggtta tcggttctct ttaaccttcg atggggtcta
     1261 tcgagatgca aagttctggc tgaatggata ttatctgggt aacaaccaaa gcggatattc
     1321 aggtgtagaa ttcgacatta ctgacttcgt ctggttcgac aaacctaata ctatagtcgt
     1381 tcgagtggat gccacccaat acgaaggttg gttttacgaa ggagcaggca tttatcggca
     1441 tgtctggctg aatcgctatc ccgaaattca tatcccttct gatggtgtct ttgcctacag
     1501 cattcccaaa ggaaagagtt gcatcattca tttcgaaact accatctcaa actcatcttt
     1561 aaatgataaa atggtgagta tggaaacaaa agtcaaaagt cgtgatggca gactaatcgc
     1621 ccaaaacatg tctaagtcgg ttcgcacggc ttcttcagga caaaccaggg taaaccaaca
     1681 gattaaaata gataatcccc acttgtggaa ccttgacgat ccttatcttt accgtatcga
     1741 aaccagtatt ctttcggaag gtaaaatctt ggataaagta agcatgccat ttggaatacg
     1801 ttttatcacc tgggatgccg acaaaggttt tttcctaaat aataaacccg taaaactatt
     1861 aggggtaaac atgcatcaag gacatgcagg cttgggtgta gccttaccga atggaatgca
     1921 atattatcgc atcaatcgtc tcaaacaaat gggagcgaat gcctaccgtt gttcgcatca
     1981 tccaccaact ctcgaattgt tggatgcctg cgatagttta ggcatgttag taattaacga
     2041 aaaccgtcta ttgaacactt cacacgaata tcttaatcaa ttcagtagaa tggtgattcg
     2101 cgacaggaat catccttcgg tcattttgtg gtcgatcggg aatgaggaag ggtatattca
     2161 aacaaatgga aacggtaaac gcatggccat taccttaat
//
LOCUS       DAORPD010000228         2198 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_9064_length_2198_cov_2.348577, whole genome shotgun sequence.
ACCESSION   DAORPD010000228 DAORPD010000000
VERSION     DAORPD010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9064_length_2198_cov_2.348577"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="PK976_03525"
     CDS             complement(<1..251)
                     /locus_tag="PK976_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HRC66989.1"
                     /translation="MIREPNFQYPFLIGTIFLFAYLIVRIILWYIELSKYDKLRFIHF
                     WKTKSVWAALKDAIADGLLHSSIFKKNLLLGYMHMSLAFG"
     gene            complement(248..586)
                     /locus_tag="PK976_03530"
     CDS             complement(248..586)
                     /locus_tag="PK976_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRC66990.1"
                     /translation="MSTFDFLKVRRLYIDYDNLDQELLNEVIREEPSFQWCISCGCCT
                     ATCSAGNHTTFNIRRISLILRRGEIEGLEEELKKCMLCGKCTLVCPRDIQLRNVVMNI
                     KKIVLKKKTA"
     gene            complement(593..1618)
                     /locus_tag="PK976_03535"
     CDS             complement(593..1618)
                     /locus_tag="PK976_03535"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRC66991.1"
                     /translation="MKKIAVIGGGIAGLEVSRLLLQEGLPVTLIEKGSRLGGNLRNYA
                     HLFPDFTPSDEILGNYRFLPSNSDLQLLMNTQIESIDFSTQPYTLHSKDGIAIPADPL
                     IIATGYQVFDAKLKEEFGYGIYDGVITSVELEKMIKQHHIVTPYGKIPRRIAFIHCVG
                     SRDAKVGNSYCSRLCCITAIKQAIETKKLLPETEIFCFYIDLRMYGLTFEELYLNAQK
                     EYNIQFVRGRLSETSEMIDKRLIIKAEDTLIGRPLQMKADMLVLMVGMEASDFSKQFA
                     YHNGLEIGDHGFFQPLNQFNARSKSQKEGIFFAGTSIGPMSVAETIEHARAAALSAIS
                     YLKDKEY"
     gene            complement(1760..>2198)
                     /locus_tag="PK976_03540"
     CDS             complement(1760..>2198)
                     /locus_tag="PK976_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HRC66992.1"
                     /translation="KAWGGEVIAYPERRHCCGFGFRQYLIKESRGYSLSHAQIKFESM
                     QPYHPDFILTNCPGCNMFMDRWQYVIQETAGKVYSSDGNGIPVLSYEELAALLLGYDP
                     IQIGLFMHQTEVLPLLEKLGIQFDKNEYSKLRAESHHNTKISR"
BASE COUNT          618 a          448 c          423 g          709 t
ORIGIN      
        1 ccaaaagcca ggctcatgtg catgtatccg agtaatagat ttttcttgaa aatgcttgaa
       61 tgcaataatc cgtccgcaat ggcatccttc aatgctgccc aaaccgattt ggttttccag
      121 aaatgaatga agcgtagctt gtcgtattta gatagttcga tataccaaag aatgattctc
      181 acaatcaaat acgcgaacaa gaatatcgtc cctataagaa atggatattg aaaattgggt
      241 tctctaatca tgctgttttc ttttttaaga cgattttttt gatattcatt actacatttc
      301 tcagttgaat atctcttgga cataccagag tgcatttgcc acagagcatg cattttttca
      361 gttcctcttc gagtccctca atttcacctc gtcgtaaaat gaggctaata cggcgaatgt
      421 taaaggtagt atgattgccg gcactgcatg ttgcggtgca gcatccacaa ctgatgcacc
      481 actgaaagga aggttcttcg cgtattactt cgttcaaaag ttcctgatcc aaattatcgt
      541 aatctatata taaacgcctt acctttaaaa aatcgaatgt tgacatttgt ttttaatatt
      601 ctttgtcttt taaatacgaa atcgctgata aagctgctgc tcgggcatgt tcaatagttt
      661 ctgctaccga catgggtcca atgcttgttc ctgcaaaaaa aattccttct ttttggcttt
      721 tgctccttgc attgaattga ttaagaggtt ggaagaaacc atgatcacct atctccaatc
      781 cattatgata ggcaaactgt ttcgaaaaat cggaagcttc catgccaacc atgagtacga
      841 gcatatcggc cttcatctgt aaaggacgcc caataagtgt atcttcggct ttaattatca
      901 accgcttatc tatcatttcc gaagtttctg acaatcgacc ccttacaaac tgaatattat
      961 attctttttg tgcatttaga tatagttctt caaaggtaag tccatacatt cttaggtcga
     1021 tataaaaaca aaatatctcg gtttctggaa gtaatttctt ggtttctatg gcttgcttga
     1081 tcgccgtaat gcagcaaaga cgtgaacagt atgaatttcc cacctttgcg tcacgcgaac
     1141 ctacacaatg aataaaagct atacgtctgg ggatctttcc ataaggagtt acaatatggt
     1201 gctgtttgat cattttttcc agttctaccg aggtaattac tccatcatag ataccgtatc
     1261 caaattcttc cttcagtttt gcatcgaaaa cttgataacc ggtggcgatg ataagagggt
     1321 cggcaggaat ggctattccg tcttttgaat gcaaggtata gggctgtgtc gaaaaatcta
     1381 tgctttctat ttgggtattc ataagaagtt gaagatcaga attacttggc aggaagcggt
     1441 aatttcccaa tatttcgtca gaaggtgtaa aatcaggaaa gagatgagca taatttctaa
     1501 ggttgccgcc caatcgtgaa cctttttcta tcagggtaac cggtaaacct tcctgcaaga
     1561 gaagtctcga aacttccagc cctgctatcc ctccgcctat tacagctatt tttttcatct
     1621 gtcgtttatg aaatttgttt tccttctaat ttactgaaat atttttctag ttgaaaaatg
     1681 aacttcatta attatcgttg gcttgttttt catggcgtca tcattttttc ggaacgatat
     1741 tgggattatc ttgatggatt tatcgggaaa ttttcgtatt atgatggctt tcggctctca
     1801 gttttgaata ttcattctta tcgaactgaa tgcccaattt ttccagcaag ggcaagacct
     1861 cggtctggtg catgaaaagg ccgatctgga taggatcata accaagcaat aatgcagcca
     1921 gttcttcata gctcaaaaca ggtatgccat tcccgtcgga tgaataaacc ttaccagcag
     1981 tttcctgaat cacatattgc cagcggtcca tgaacatgtt gcagccaggg caattggtaa
     2041 ggatgaaatc gggatgatag ggttgcatgc tttcaaattt aatctgagcg tgagagagag
     2101 aatagcctct gctttccttt attaagtatt gcctaaaacc aaagccgcaa cagtgcctgc
     2161 gttcaggata agcgatcact tcgccgcccc aggctttt
//
LOCUS       DAORPD010000229         2197 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_9067_length_2197_cov_2.524276, whole genome shotgun sequence.
ACCESSION   DAORPD010000229 DAORPD010000000
VERSION     DAORPD010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9067_length_2197_cov_2.524276"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="PK976_03545"
     CDS             <1..750
                     /locus_tag="PK976_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HRC66993.1"
                     /translation="DIKVFIQYGMITEPKFYERAEKFYLFKDIDHKYYTFEEYKALIK
                     PLQTDKEKNLVYIYSNNPETEYVFISAAKEKGYNILLMDGILDNHFINHLEMKFDKCR
                     FVRVDSDVIEKLIPKEEESISKLTEEQKENLKKLFEEVIDKNNFTLVLENLSESDLPV
                     FITKPEYMRRMKDMSALGGGMSYMDALPDQFNLVINTNHPAILHILDENDEEKRKNLA
                     HQLKDLAMLQQGLLKGEALTDFVKRSVEFIR"
     gene            894..1484
                     /locus_tag="PK976_03550"
     CDS             894..1484
                     /locus_tag="PK976_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007757729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HRC66994.1"
                     /translation="MKKGLIIGLSIAIIVLIFILTGVSMYNSLVKAEENVNAQWANVE
                     NVYQRRADLIPNLVNTVKGYANFEQKTITDVIEARAKATSVNINPEKLNEASLQEFQN
                     AQEQLSSALSRLMVVVERYPELKANQNFLELQSQLEGTENRIAVERRKFNEMAQAYNK
                     SIRTFPRNIIANMFRFERKAYFEATEGAEQVPKVEF"
     gene            1488..1955
                     /locus_tag="PK976_03555"
     CDS             1488..1955
                     /locus_tag="PK976_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HRC66995.1"
                     /translation="MALLKIKSVRDYFTPEERERLTEAVKEMELKTSAEIRIHIERKC
                     QGDPLDRAAYIFGKLGMHKTALRNGVLIYLAIENHQFAFIGDRGINAVVPPNFWTDLK
                     LKAQKYFSEGQFTQGLLEVIEEIGTYLQKFFPYKENDINELPDEISFKDKPSK"
     gene            1969..>2197
                     /locus_tag="PK976_03560"
     CDS             1969..>2197
                     /locus_tag="PK976_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HRC66996.1"
                     /translation="MQDNSLSKGLRTLAFLLFTSLFFITSLKADDLPPRPFPPRLVND
                     LAGIIDPYAIIQLEQKLVAFNDTTSTQIAVVT"
BASE COUNT          744 a          452 c          426 g          575 t
ORIGIN      
        1 gatatcaaag tctttataca atatggaatg attactgaac caaaattcta cgaaagagcc
       61 gaaaaattct acctcttcaa ggatatcgac cataaatatt acactttcga agaatacaaa
      121 gccttgataa aacctttgca aaccgataag gaaaaaaatc tggtatatat atacagcaat
      181 aatccggaaa ccgaatatgt attcatttcg gctgcaaaag aaaaaggcta caatattttg
      241 cttatggatg gaatcctcga caaccatttc atcaatcatc tcgagatgaa attcgacaaa
      301 tgcaggtttg taagagtaga ttctgatgtc atcgaaaaac tgattcccaa ggaagaagaa
      361 agcatttcta agttaactga agaacaaaaa gaaaacctga aaaagctttt cgaagaagtc
      421 atcgacaaaa ataatttcac tctcgtgctc gagaatctgt cggagagcga cctacctgtt
      481 ttcatcacca agcctgaata catgcggcgt atgaaggata tgtcagcctt gggagggggc
      541 atgtcctata tggatgctct tcctgatcag ttcaatctgg taattaatac caatcacccg
      601 gctattctgc atattctcga cgaaaacgac gaggagaaac gcaagaatct ggctcatcaa
      661 ctgaaagatc tggctatgct gcaacaaggc cttttaaaag gcgaagcgct gaccgatttt
      721 gtaaaacgtt cggtagaatt cattcgctaa aaaaccctcc aacttgccaa gaaaaaacga
      781 ttgactcaat aagaggatga ttcatctctg attttgattt tttttgactt caaatacaaa
      841 aagagtactt ttgtaggaag taattcttca aaataacctt taaattcaaa tttatgaaaa
      901 aaggactgat aattggattg tcgattgcca ttatcgtact gatattcatt ttaacaggcg
      961 tttcgatgta caactcgctg gtaaaagctg aagaaaatgt aaatgcgcag tgggcaaatg
     1021 tggagaatgt atatcagcgc cgtgccgatc ttattcccaa tttggtgaat acggtgaaag
     1081 gttatgccaa tttcgagcag aaaacgataa ccgatgtcat tgaagctcgt gcaaaagcca
     1141 cttctgtaaa catcaatccc gaaaaattga atgaagccag cctgcaggaa tttcaaaatg
     1201 cgcaggaaca actgagttca gccctgagcc gtttgatggt tgtggtggaa cgttaccctg
     1261 agctgaaagc caatcagaat tttcttgaat tgcagtcgca gctcgaaggt accgaaaacc
     1321 gcattgctgt ggaaagaaga aaattcaacg agatggctca ggcgtataac aaaagcatac
     1381 gcacctttcc tcgaaacatc attgccaaca tgtttagatt cgaaaggaaa gcctatttcg
     1441 aggctactga aggagccgag caagtgccta aagtcgagtt ttaaaaaatg gctctcttaa
     1501 aaataaaatc ggtacgcgat tattttacac ccgaggaacg cgaacgcctt acagaagcag
     1561 taaaagaaat ggagctgaaa acctcggcag agattcgaat tcatatagag cgaaaatgcc
     1621 agggtgatcc actcgaccgt gctgcctata tttttgggaa acttggtatg cataaaacgg
     1681 cacttcgcaa tggagtactg atctatcttg ccatagaaaa tcaccaattt gctttcattg
     1741 gagatagagg aatcaatgcg gtagttcctc ccaacttctg gacagattta aaactaaaag
     1801 cacagaaata tttcagtgaa ggacaattca cacaaggttt gctcgaagtg attgaagaga
     1861 tcggtactta tctccaaaaa ttctttccct ataaagaaaa cgatatcaat gaattacccg
     1921 atgagatctc attcaaagat aaaccatcga aataattcga agcctatttt gcaagacaat
     1981 tcactgagca aaggccttag aactttagca tttcttctat tcacctctct ttttttcatt
     2041 acaagcctga aggccgacga cctccctcct cgtcctttcc ctcctcgcct ggtgaatgat
     2101 cttgcaggaa tcatcgatcc ttacgccatc attcaactcg aacaaaaact ggtagcattc
     2161 aacgatacta catccactca aatcgccgta gttactg
//
LOCUS       DAORPD010000230         2179 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_9180_length_2179_cov_1.935499, whole genome shotgun sequence.
ACCESSION   DAORPD010000230 DAORPD010000000
VERSION     DAORPD010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9180_length_2179_cov_1.935499"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1622
                     /locus_tag="PK976_03565"
     CDS             <1..1622
                     /locus_tag="PK976_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66997.1"
                     /translation="DVQRSNITSQSAKTTPSIAQTTPSAIELQQQKNSLNPSEEESDF
                     SSAEQQYSTRIAEGGEIVSPPDRILYNEYYDENDEFDFDDYYDYAYSVRIKRFHRPVY
                     GAGYYDDCYTNLYWYTYDPWVWGMSVYLGYDWWWWDWYGGPYFSLGYNWGWGSINWHW
                     GFPYSGWYYYPHFFYHYPWYPWYYGWGAYPYYIGWWNGFWFGHYYNSHDPNSYYYGRR
                     VSRHETNGAASGGTFGEYYENYVNNQRRGGQGTNGVTQPSGQGSESSLTQRRIQAAGV
                     NQQAVKDKPGEEKNLTQGTLNERRISASESTQVTPKTDQPAVTPARIRSGEENLAAPK
                     PSNEQSEGNVSNRRISGNVNQTAEQSQTQDSRLRQTQENYAKPQASPANPQQYDYNRY
                     RRTSPATNPEQLARPKTQGNQNDRPVRTYTPPTYSRPRSSNEYTTPRRITIPQQSEQP
                     SQMNQGVKRETQKSSERNKSYTPPSSSRPNYSVPSSSNSHQNNSSRPSYNSSSSQERH
                     SSSSFSSPSRSSSNSGGGSSSSSRSGSGRRR"
     gene            1711..>2179
                     /locus_tag="PK976_03570"
     CDS             1711..>2179
                     /locus_tag="PK976_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66998.1"
                     /translation="MRKISLMGILLFATVMVFAQNATDALRYSSVNYFGTTRFMTMGG
                     AFGAVGADFSVLSTNPAGLGLYKSSEVCFTPLIYHGQTKSSFWANKAEDDRYNFAFSN
                     MGAVLTFEFPSQLETPSWRYVQFGFGVNRLLDFHNRIMVEGFNNDNSLLTGYVE"
BASE COUNT          653 a          500 c          457 g          569 t
ORIGIN      
        1 acgatgtaca aaggagcaat attacgtccc aatccgctaa aactactcca tccatagcac
       61 aaacaacccc ctcagctatt gaattacaac aacaaaagaa ttcgctgaat ccttcggaag
      121 aggaaagcga tttttcttcg gccgagcaac aatattccac tcgaattgct gaaggcggtg
      181 aaatcgtttc tcctcccgac cgtattctct ataacgaata ttatgatgaa aacgatgaat
      241 tcgacttcga cgactattac gactatgctt atagcgttag aataaagcgt ttccatcgcc
      301 ctgtctatgg cgcaggttat tatgatgatt gttatacgaa tttatactgg tatacttacg
      361 atccctgggt ttggggaatg agtgtatatc ttggatatga ctggtggtgg tgggattggt
      421 atggaggccc ttatttctct ctgggatata actggggctg gggcagtata aactggcatt
      481 ggggattccc ctattcgggc tggtattatt atccacactt cttttaccat tatccatggt
      541 atccctggta ttatggatgg ggagcttacc cttattacat tggttggtgg aatggtttct
      601 ggtttggaca ttattacaac agccacgacc ccaactccta ctattatggt cgcagagttt
      661 cccgtcacga aactaacggt gcggcaagcg gtggaacctt cggcgaatat tacgagaatt
      721 acgtaaacaa tcaaagaaga ggaggccaag gcactaatgg agtgactcag ccatcgggcc
      781 agggatcgga atcttcactg actcaaagaa gaattcaagc tgcgggagtt aatcagcagg
      841 ctgtaaagga taaaccaggc gaagaaaaaa atctgacgca aggaacgttg aatgaacgtc
      901 gtatttcggc gagtgaatca actcaagtaa cacctaaaac cgatcaaccc gctgtaacac
      961 ctgcacgcat tcgttcgggc gaagaaaatc ttgcagcacc taaaccatcc aatgaacaaa
     1021 gcgaaggaaa tgtttcgaat cgaagaatct caggaaacgt gaatcagacc gctgaacaaa
     1081 gtcagaccca ggattctcgc ttgcgtcaaa ctcaggaaaa ttatgcaaaa cctcaagctt
     1141 cacctgccaa cccacaacaa tacgattaca atcgttatcg tagaacttcg cctgccacga
     1201 atccagaaca acttgcccgt cctaagactc aaggaaatca aaatgacaga cctgttcgca
     1261 cctatacccc ccctacttat tcacgcccaa gaagtagtaa cgaatataca actccccgac
     1321 gcattaccat accccaacaa agtgagcaac cttcacaaat gaatcaggga gttaaacgtg
     1381 aaacccaaaa gtcttctgag aggaataaaa gctatacccc tccttccagc agtcggccta
     1441 attattctgt cccttccagt tctaattctc accagaataa ttccagcagg ccgtcctata
     1501 acagctcatc ttcacaggaa cgtcactctt cttcctcatt cagttcgcct tcccgatcga
     1561 gctccaatag tggaggagga tcatcctcct catcgagaag tggtagtggc cgaagaagat
     1621 aatcaaatcg aaataacctc ataaaatcgt ataaaagaat tttttttacc attcgcaaat
     1681 tcattcaaac catataaaaa agaatagtct atgagaaaaa taagtttgat gggaatatta
     1741 ctgtttgcca cagtgatggt ttttgcacag aatgctacgg acgctttgcg ttattcatcg
     1801 gtcaattatt ttggtactac ccgtttcatg acgatgggtg gcgcatttgg agcggttgga
     1861 gccgattttt cggtgttgag caccaatccc gcaggcttag gtttatacaa aagctccgaa
     1921 gtatgtttta ctcccttgat ttatcacggg caaaccaaat cgagtttctg ggctaataag
     1981 gccgaagatg acagatataa ttttgccttc agcaatatgg gagccgtgtt gacattcgaa
     2041 tttcccagtc aactcgaaac cccaagctgg cgctatgttc agtttggatt tggggtgaat
     2101 cgactgctcg attttcataa ccgtatcatg gtggaaggat ttaacaatga taattccctt
     2161 ctgacaggtt atgtagagt
//
LOCUS       DAORPD010000231         2174 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_9221_length_2174_cov_2.457763, whole genome shotgun sequence.
ACCESSION   DAORPD010000231 DAORPD010000000
VERSION     DAORPD010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9221_length_2174_cov_2.457763"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="PK976_03575"
     CDS             <1..405
                     /locus_tag="PK976_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRC66999.1"
                     /translation="QCGFVLVNKNDELLVQGRPMDISMLRAAAKEFLANPYNRPDLPE
                     KKMEIIPGLGEVETSKGVISLKNDRGTSYKMYIKVQNELTAAFNELRDELSMERFGVK
                     FSQLSNQQYIDAIQKAIPMSISEAEPENVGGK"
     gene            408..872
                     /locus_tag="PK976_03580"
     CDS             408..872
                     /locus_tag="PK976_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005781259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRC67000.1"
                     /translation="MARFKKSEGRSTPGINTGSMSDIIFMFLFFFMVITTIRESSLIV
                     RVKVPQATEVAKLERKSLVSFIYVGQPIDYKYGTESRIQLNNQFASVADIREFITRER
                     EARDEVDRPLLTTSLKVDEETRMGIVTDIKQELRQVGAFKINYSARKTLAKE"
     gene            1161..1970
                     /locus_tag="PK976_03585"
     CDS             1161..1970
                     /locus_tag="PK976_03585"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRC67001.1"
                     /translation="MPFMSFQNHPVYYESRGLGPAIVFLHGFMESHKIWESFANSFSN
                     EFQSITFDLPGHDQSDIIAPVHGMELMADCTKSILDHLGVKDCILVGHSMGGYVSLAF
                     ADKFPERVKGLVLFHSSALPDSDEGRKNRERTIEFIQQNKTGFILQFIPSLFAPSNAE
                     KYQMEIQMLIESASSMKPQAIIAAQIGMKERPSYLDVLTHIQAPVLFIIGKQDSRISA
                     KDYFSQIFLPSHSEILVLENVGHNGYIEAFPVTSAAIRHFAQRAFELYSAI"
BASE COUNT          654 a          437 c          416 g          667 t
ORIGIN      
        1 caatgtgggt ttgttttggt aaataaaaat gacgaactgc ttgtacaagg ccgacctatg
       61 gatattagta tgcttagagc cgctgctaag gagttccttg caaatcccta taatcgtcca
      121 gatctccctg aaaagaaaat ggagataatt cctggtttag gagaagtcga aacatcgaaa
      181 ggcgttatat cactaaaaaa tgaccgaggt acatcctata aaatgtacat taaagttcag
      241 aacgagctaa ctgcagcttt taatgagctg cgcgacgagc tttcaatgga acgctttggg
      301 gtgaaattta gccaattatc aaatcagcaa tatatcgatg ctatacaaaa agctattcca
      361 atgtcgattt cagaagccga acccgaaaac gtaggaggta aataattatg gcaagattta
      421 aaaaatcgga aggacgttca acaccaggaa tcaatacagg ttcaatgtcg gatattattt
      481 ttatgttcct gttttttttc atggttatta ctaccattag agaaagttct ttaattgtaa
      541 gagtaaaagt tcctcaggct accgaagtcg caaagctcga aagaaaatct ttggttagtt
      601 tcatttatgt aggacaaccc attgattata aatacggtac agaaagccgc attcagctca
      661 ataatcagtt tgcttcggtg gccgatattc gtgaatttat tactcgcgaa agagaagccc
      721 gtgatgaagt ggatagacct ctacttacaa cttcattaaa agtggacgaa gaaacccgta
      781 tgggaatcgt aacagatatc aaacaggaac ttaggcaggt gggagcattc aagatcaact
      841 attctgcccg aaaaaccctt gctaaagaat aatctttcct cttcatgact aattattagg
      901 aggccctcag gtatcatgcc taagggcctc tttttagcat cgatacatca atcccaaata
      961 tccactcaaa acttatgata gcccggttgg ctcaataggc cttaaggcac tatccaaaaa
     1021 ccaataaagg tagattcgtc atcgaaattt ataccttcat gaaaaattgg agatgtaaaa
     1081 ctacttcgtt catttggctc accttgtctt attagacaaa aatctcctaa attcgcacat
     1141 ttgttcaaac tctcgtttct atgcctttca tgtcatttca aaatcatccc gtttattatg
     1201 aaagccgagg tctagggccg gctattgttt ttctccatgg cttcatggaa tctcataaaa
     1261 tctgggaatc ttttgctaat tcattctcca atgaatttca atcaattacc ttcgatttgc
     1321 cgggacatga ccaaagcgat atcattgctc ctgtccatgg tatggaacta atggctgatt
     1381 gcactaagag tatattggac catctggggg tgaaagattg catcctggtc gggcattcta
     1441 tgggaggtta tgtttcgctt gcctttgctg ataaattccc tgagcgggta aaagggcttg
     1501 ttttgtttca ttcttctgct ttacccgatt ctgatgaagg ccgcaaaaat cgtgaacgga
     1561 caattgaatt cattcaacaa aacaaaacgg gatttatttt gcaatttatt ccatcattat
     1621 ttgctccgag taatgcggaa aaatatcaaa tggaaatcca aatgctcatc gagtcggcct
     1681 cgtccatgaa gccgcaagct attattgccg ctcaaatagg aatgaaggaa agaccttctt
     1741 atttggacgt tcttactcat atccaggcac ctgttctttt cattattggt aagcaggaca
     1801 gccgcatatc ggccaaagat tatttttccc aaatttttct tccctcacat tctgagatcc
     1861 tcgtactcga aaatgtcggc cataacggat atatcgaggc tttccctgtt acctcggctg
     1921 ctatccgaca tttcgcacaa cgagcattcg agttgtattc ggctatctga ttatttcatg
     1981 aagttgcttc tcctttcttg tttccttctt tattgttagg gaaaatgccg acgaattaat
     2041 aatggaaaaa attttggcaa aggcgattat aaaaatttga ctgcgaatta tgtatttaca
     2101 tctattttct caggacatag ttacatcaca agttcttctt ccttctgtaa gattgaaatt
     2161 agcctaagcg cttg
//
LOCUS       DAORPD010000232         2174 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_9222_length_2174_cov_2.253893, whole genome shotgun sequence.
ACCESSION   DAORPD010000232 DAORPD010000000
VERSION     DAORPD010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9222_length_2174_cov_2.253893"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..1172)
                     /locus_tag="PK976_03590"
     CDS             complement(135..1172)
                     /locus_tag="PK976_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008775385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HRC67002.1"
                     /translation="MVKRFKNNPLLCPSDIEPSMPDMEVACLLNPGVFRYEDKIWLVL
                     RVAERPKQEEGKISFPVYNDKGKIEILSFQKDDTDLDAHDSRVIRYKGSDYLTTLSHL
                     RLVCSEDGIHFMEPEGYSSIMGQGPLESFGIEDCRVATFDDFYALTFTEVSRYGVGVG
                     LIITQNWKDYQREGMIFPPHNKDSAIFEQKIDGIYYAFHRPSSPELGGNYLWIAESPD
                     LIHWGKHRCIATTRERMWDSARIGAGASPILTPEGWLEIYHGANEKNEYCLGVILLDK
                     KYPWKVLARSQEPIMQPEMEYELKGFFGHVIFTNGHLVEGDTIKMYYGASDEVICGAT
                     LSISEIMNTLK"
     gene            complement(1479..>2174)
                     /locus_tag="PK976_03595"
     CDS             complement(1479..>2174)
                     /locus_tag="PK976_03595"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRC67003.1"
                     /translation="QEIFIEGHIVNLNHQPVSYAHVILLRNLTGTVSDSAGRFMMPLK
                     YGDTLIISHVGYATRVYSLNESSLLYDHRLEIVLLDKIYELKEVVITTFPKTWEEFKY
                     AFENLKGDEEEQPADLQLNKEIPLGYNIPQSGFGITMNGPITALYNIFSHEGKSRRKL
                     ARLLENDKRNRAVSQRYNYDLIKKLTGITQQETIERFMQFCHLSDEFVLQSSDYELYL
                     AILECYREFCEES"
BASE COUNT          628 a          475 c          396 g          675 t
ORIGIN      
        1 cagaaaaatt tcgactacgg gaagcacgag ggaatataac atgttggtta tcaacaaaac
       61 cctcatgttg tatggcatcg aaagaaaatg ctcgtattct attttgatct tcttcagcat
      121 attttcgaat atgattactt cagcgtattc attatttcag agatagacaa agtagcgcca
      181 caaatcactt catcactggc accataatac atcttgatgg tatcaccttc caccagatgg
      241 ccgttggtaa aaattacatg accaaagaaa cccttcagtt cgtactccat ttcaggctgc
      301 atgatgggtt cttgacttct ggccagaact ttccatggat attttttatc gagaagaatt
      361 actccgagac agtattcatt tttttcgttt gcaccatgat agatttcgag ccaaccttca
      421 ggagtgagta tgggcgaagc gcctgctcca atacgagccg aatcccacat tctttcacgg
      481 gttgttgcta tacatcgatg ttttccccaa tgaatcaaat cgggagactc ggcaatccaa
      541 agataatttc ctcctaactc aggactactg gggcgatgaa aagcataata tattccgtcg
      601 attttttgtt cgaaaatcgc actgtccttg ttatgaggtg gaaaaatcat cccttcacgc
      661 tgataatcct tccagttttg agtaattatc aaacccacac ctacaccata tcgcgaaacc
      721 tcggtaaaag tcaacgcata gaaatcatcg aaagtagcta cccggcaatc ttctattcca
      781 aacgattcta aaggcccttg tcccatgatg gaagaataac cttcaggttc cataaaatgt
      841 atcccatcct ccgaacaaac cagtcgtaaa tgcgacagcg ttgtaagata atcgctgcct
      901 ttgtaccgta ttacccttga atcatgggca tccagatcgg tatcatcttt ttggaacgaa
      961 agaatctcga ttttaccttt atcgttatag acaggaaaag aaatcttacc ttcttcctgt
     1021 tttggacgtt ccgcaaccct tagtaccaac catatcttat cttcatatct aaatacgccg
     1081 ggattcaata aacaggcaac ctccatgtcc ggcatagatg gctcgatatc tgaaggacaa
     1141 agaagtggat tatttttaaa tcgtttcacc atggcttgat ttgttttgct caaaattact
     1201 gatactttgt gccagagcgt gtccgctcag taacatattt tgtccgttat gttatgaatt
     1261 ttattaggaa ggtggcaaga tttccagggt atgtgttgct tggggataca gagattgtgg
     1321 gatgaaagga ctcctattaa caagcaacta attcattttt agcttattga cttttaactt
     1381 tcggctctct ataccccatc taccatcctc tgtcctgtct attcttaaat taggttggcc
     1441 attttacaaa ttctggtgta gaatcgagtg ccactgccct aactttcctc acaaaattca
     1501 cgatagcatt ccaaaatggc cagatacagc tcgtaatcgg aagattgcag aacaaattca
     1561 tcggatagat ggcaaaactg catgaatctt tctatggttt cttgctgggt aatacccgtg
     1621 agttttttta tcaaatcata gttatatcgc tggctaacgg ctctattacg cttgtcgttt
     1681 tccagtaaac gggctaactt cctcctgctt ttgccttcat ggctaaaaat attgtacaat
     1741 gcagtaatcg gaccattcat tgtaattcca aaaccacttt ggggaatatt atatcccaag
     1801 ggtatttcct tattaagttg caaatcagca ggttgctcct cttcatcgcc tttcaggttc
     1861 tcaaaggcat atttgaattc ttcccatgtt tttggaaacg tagtaataac cacttctttc
     1921 agttcatata tcttgtccaa cagaacgatt tcaagcctat gatcgtaaag taaagaagat
     1981 tcattcaagg aataaactcg ggttgcatat cctacatgac tgataatgag tgtgtcacca
     2041 tattttaacg gcatcatgaa acgccctgcc gaatcgctta ccgttccagt taagttgcgt
     2101 aataaaataa catgcgcata agaaacaggt tgatgattga ggttgacaat atgtccctcg
     2161 atgaaaattt cttg
//
LOCUS       DAORPD010000233         2172 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_9235_length_2172_cov_2.672178, whole genome shotgun sequence.
ACCESSION   DAORPD010000233 DAORPD010000000
VERSION     DAORPD010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9235_length_2172_cov_2.672178"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..>2172)
                     /locus_tag="PK976_03600"
     CDS             complement(176..>2172)
                     /locus_tag="PK976_03600"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HRC67004.1"
                     /translation="LEIPCSQIAEHAGGELAEAIERLRQRRVMITPGYDGEYGTIKVF
                     RPGEAQSYAQKSSLFHDENPLNRPTAYPLLSFNIHHVLEQKIRYSAIPDSSSEQDTVY
                     PTTDDLQKAAIAHDEGPAIAIAGPGSGKTYVLTERIARLISQNIAPSEKILAITFTTR
                     AAQEMRQRLMSHPSLHQQASSLSISTIHALGLQILREIPSYSSFKLISETEKIKIIQS
                     LLQVQNYSPVKIVNYISLLKNHALNFRFDVTFQKIANQYQFYLLNNQLMDYEDLILLP
                     LNFLQEDAEFRSQIQNRNGFILIDEFQDVNPAQYEFLKIISHPQENNVFAIGDPFQSI
                     YGFRGAYSGIFETFLQDFPQATTYHMGKSYRCGDKILKAAYEVIGSENPLEGLPEKGK
                     VNIVQHATDKSEAEFIARSIDAMTGGTRFFAYDSGVAQAQTEIPLSEFTILCRTTRQF
                     PAIIAALNNHGLPYQTAQAGNLLDSELLSEIILYLRKILENPEENKCTVENLLKAYFE
                     QNLSEKNDTIDNRNIFMEIAKDFGNDLKSFIDFIDLNAPTGLYEAKENAVQLMTLHAA
                     KGLEFEHVFISGCEEGLIPYSLFENQETDPEEEKRLLFVGMTRAKKTLTLNYADSRKI
                     FHRNMKLPPSHFLAAISPANAIRTKSNLPEKHEGQQLSFF"
BASE COUNT          581 a          379 c          523 g          689 t
ORIGIN      
        1 taacgaaatc aaccattata gatttatgat tcatgattcg aaaactatgt tattacgtga
       61 tattttatgt gcttataatt ttagttggtt gggatattta tatcaatgac tcatctaaat
      121 tcttttgtta agatgaattt caaagccaaa ttcaagtaga atgcatgcgg aaagatcaga
      181 agaaacttag ttgctgtccc tcgtgttttt cgggcagatt ggattttgtc ctgatggcat
      241 tggctggcga aatggctgca agaaaatggc tagggggaag tttcatgttt cggtggaaaa
      301 tcttgcgtga gtcggcatag tttaaggtaa gtgttttttt ggcacgagtc attcctacga
      361 aaagcaggcg tttttcttct tcagggtcag tttcctgatt ttcgaaaagg gaatagggaa
      421 taaggccttc ttcgcaaccg ctgataaaaa catgttcaaa ttcaaggcct ttagctgcat
      481 ggagcgtcat aagctgaact gcattttctt tggcttcata aagtcctgtg ggagcattca
      541 aatcgatgaa gtctatgaaa cttttcaaat cattcccgaa atctttggca atttccatga
      601 aaatattccg gttatcaatg gtgtcgtttt tttctgagag attttgttcg aaataggctt
      661 tcagaagatt ttcaactgtg catttatttt cctcagggtt ttcgaggatt ttacgcaagt
      721 agagtatgat ttccgagagc aattccgaat cgagcaaatt tcctgcttga gcggtttgat
      781 agggaaggcc atgattgttc aaagcagcga tgatggccgg gaactgtcgc gttgtacggc
      841 ataagatggt gaattcagat aatgggattt cagtttgagc ttgcgctacg ccgctgtcgt
      901 aagcgaaaaa acgcgtgcct cccgtcatgg catctataga tcgagcaata aattcggctt
      961 cacttttgtc ggtagcatgt tgcacaatgt tcactttgcc tttctcgggc aagccttcga
     1021 ggggattttc actgccaatg acttcatagg cagccttcaa gattttatct ccgcaacgat
     1081 agcttttccc catgtggtaa gtagtcgctt ggggaaaatc ctgaagaaaa gtttcgaaaa
     1141 tacccgaata tgctcctcta aaaccataaa tactttgaaa aggatctcca atggcaaaaa
     1201 cattattttc ttgtggatga ctgatgatct ttagaaattc gtattgagct ggatttacat
     1261 cctgaaattc atctattaaa ataaacccat tacgattttg gatctgcgaa cggaattcgg
     1321 catcttcttg caagaaattc aaaggcaata agataagatc ttcgtagtcc atcaattgat
     1381 tgttgagaag atagaattga tattgattgg cgattttctg aaatgttaca tcaaaccgaa
     1441 agttaagggc atgattttta aggagggaaa tgtagttgac gattttaacc gggctgtaat
     1501 tttggacctg aagaagtgat tgaatgattt ttattttttc ggtttcgctg attagtttga
     1561 aggaagaata agaggggatt tcgcggagaa tttgcaagcc caaggcatga atcgtactga
     1621 tggagagaga tgatgcttgt tgatggagtg aaggatggga cataaggcgt tggcgcattt
     1681 cctgagctgc ccgtgtggta aaggtaatgg caagaatttt ttcggaaggt gcaatatttt
     1741 gtgatatcag gcgagcgatt ctttcggtga gtacgtatgt ttttccgctt cccgggcctg
     1801 ctatggcaat ggctggtcct tcgtcgtgtg cgatagcagc tttttgcaag tcatccgtgg
     1861 taggataaac tgtatcctgt tcgcttgagg aatctggaat tgccgaataa cgtatttttt
     1921 gttcgagcac atgatggatg ttgaaactga gcaggggata ggctgttgga cgatttaatg
     1981 ggttttcatc gtgaaatagt gaagattttt gggcatagct ttgtgcttcg cctgggcgaa
     2041 acactttaat ggtgccatat tccccgtcat atcctggagt aatcattacc cggcgctggc
     2101 gcaacctttc gatcgcttcg gccagctcac caccagcgtg ttcggcaatc tgtgagcaag
     2161 gtatctctaa aa
//
LOCUS       DAORPD010000234         2165 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_9278_length_2165_cov_2.741232, whole genome shotgun sequence.
ACCESSION   DAORPD010000234 DAORPD010000000
VERSION     DAORPD010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9278_length_2165_cov_2.741232"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..1453)
                     /locus_tag="PK976_03605"
     CDS             complement(86..1453)
                     /locus_tag="PK976_03605"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HRC67005.1"
                     /translation="MNRKLSYLFSALLMLIGIPLWAQKEADTIRYNQYGVAVDRIPLI
                     AERRGGVLVFESRDQKQRIWFDTRVQVDGAIFTGDTYNEIGNGTSIRRARLAMKTELN
                     EKWYGELDLDFANSELELKDAYLAYIPNEHWIIRTGNFKESFSMESTTTSRYLTFMER
                     PMAVSAFAPSRHIGMALQYNYNWLLAIGGIYFQDVSDLEERTYSVDNNKNLGIDEGYS
                     LTGKLVFMPLNNKSGYGIHLGASTSYRTPKTDAEIPGSVRFSTRSLTSINRKKYLDTD
                     NILNVDYTWQNGFELAAYYHNFRIQGEYLLTDVHRKNDLPTENFNGFYVMGSCMLFGG
                     EYRYNQSDGEFTQPSRGRKWGDIELALRYDMLDLNSRMDGVMGGAGEGITAGINYHVN
                     SSIKVMFNYAYLNHDRYANGKGKLFVGHDILGNLTKNPSDVVEAEGKAGEDFSMFSIR
                     FEIDF"
     gene            complement(1951..>2165)
                     /locus_tag="PK976_03610"
     CDS             complement(1951..>2165)
                     /locus_tag="PK976_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67006.1"
                     /translation="YSFSFGCSIHTLNYNIALYPLHNFHFLHQPPKCPATRNIFRLFS
                     LTTISHSPCTKHRVPLSIPRLVENRK"
BASE COUNT          666 a          422 c          417 g          660 t
ORIGIN      
        1 cttataacga actaaaaccg tacctttgcc gttattaaaa agcatgttct tttcatattt
       61 caaaaaggtt aagagagttg aacaattaaa aatcgatttc gaaacgaatg gaaaacatcg
      121 aaaaatcttc cccagctttc ccttcggctt caaccacatc ggaaggattt tttgtaagat
      181 tgcctaaaat atcatgtcct acgaatagtt ttcctttccc gttagcgtat ctgtcgtgat
      241 tgagataagc atagttgaac ataaccttta tgctggaatt tacatgataa ttgatacctg
      301 ctgtaattcc ctcgccagct ccgcccatta cgccatccat gcgactgttg aggtctaaca
      361 tatcgtagcg aagggcaagt tctatatcac cccatttcct tcctctcgaa ggttgggtaa
      421 actcgccatc actttgatta tatcgatatt cacctccaaa aagcatacag cttcccatga
      481 cataaaatcc gttgaagttt tcggtgggca aatcattttt gcgatggaca tcagtcaata
      541 ggtattctcc ctgtatcctg aaattatgat agtaggccgc cagctcgaaa ccattttgcc
      601 atgtataatc cacattgagt atgttatcgg tatcgagata tttcttgcga ttgatactag
      661 taagagaacg cgtactgaaa cgaacactcc ccggaatctc ggcgtcggtt ttgggagtac
      721 ggtaagaagt tgatgcgcct aaatgaatcc catagccaga tttattgtta aggggcatga
      781 agacaagttt acccgtaagg gaatatccct cgtctatacc aaggttcttg ttattgtcga
      841 ccgaataagt tcgttcttcc agatcactta cgtcctgaaa gtagatgcct ccaatagcca
      901 gtaaccaatt gtaattatat tgcaacgcca taccaatatg acgtgaagga gcaaaagccg
      961 atacagccat tggcctttcc atgaaagtta agtagcgaga ggtggtagta gattccatcg
     1021 aaaagctttc cttgaagtta ccagttctta ttatccaatg ctcgttagga atgtatgcta
     1081 ggtaagcatc cttgagttcg agttcagaat tggcaaagtc aaggtctaat tccccatacc
     1141 atttctcgtt taattcggtt ttcatggcca gacgagcccg gcgtatcgat gtgccattcc
     1201 ctatctcatt gtaagtatca ccggtaaata tagcgccatc tacctgaacg cgtgtatcga
     1261 accagattct ctgcttctgg tcacgacttt cgaatacaag tactccacct ctgcgttctg
     1321 caatcagtgg aatacgatct acagcaacac catactgatt gtatcgaatg gtgtcagcct
     1381 ccttttgagc ccacaaggga attcctataa gcatgagaag ggcagaaaat aaataagaga
     1441 gttttcggtt cataagattt tatttttaaa gtgagggatt catgatggca aatttaagta
     1501 aaatagcaga ttattagtaa gataatgctt attccgataa aatatttcca tgtaaggtaa
     1561 cttcaatttt aagaaaaggt tccgaaaaac aacacgatat tcttttcatc ggattttcaa
     1621 atttacggtt ataatcatta tcaatacttt aaagaccaaa tatcaacaat tttttactcc
     1681 ccaaacaagg tattttcata gcatagacaa tatggccgtt ctattcgaaa attattggta
     1741 agtgttagca agttgtaatc ctaagacgaa atatcaacca tctttcccca attgattgat
     1801 taacaatata ttttcaaaat ttcaaacttt tacaggctac taaaagaagc ttattattta
     1861 acactttttt ttacgccgat atatccaatt ttttatgaaa ccattgatat acatggcgac
     1921 ttcatccata atggtttgaa acaacaaatt tcattttcta ttttcgacaa ggcgtggaat
     1981 actaagtggt acgcggtgct tagtacatgg cgaatgggag atggtggtga gtgaaaaaag
     2041 gcgaaagatg ttgcgtgtgg cggggcattt tggaggttgg tgtaaaaaat gaaagttgtg
     2101 taaggggtat aatgcaatgt tatagtttaa ggtatgtatc gaacagccga acgagaatga
     2161 ataag
//
LOCUS       DAORPD010000235         2165 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_9279_length_2165_cov_2.398104, whole genome shotgun sequence.
ACCESSION   DAORPD010000235 DAORPD010000000
VERSION     DAORPD010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9279_length_2165_cov_2.398104"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1313
                     /locus_tag="PK976_03615"
     CDS             <1..1313
                     /locus_tag="PK976_03615"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HRC67007.1"
                     /translation="TAGISLTRHGHLDCYIYLSRAEVQGFTIETEVTNTGGDLGLSGN
                     LVYTNRNVFRGAEMLRLKLRGAAEMQKSFEQTSQNKLLLFNTYESGAELNLTFPRFVW
                     PLKGLRFPSSMRTKSNLLVGYNYQNRPDYIRHITNAAFSYQLKVSDFKSHLLTPLELN
                     AIKIYATPSFQQLIDSIDDQGIRSQYSDHLIPALRYSYIFTNQEIGKFHNYKYFRFDF
                     ESSGNTLYAVDRLTNVSPNIHNEYTFLGIAYAQYLKASSDFRYFYMFNLNNTLVMRIF
                     AGAAIPYGNSNYLPFEKGFFAGGANGMRGWTLRMLGPGHFSSEGGQRYNRMGDLHFET
                     NLEYRFPMYNFFKGAFFLDAGNIWLVKSNPMYPGGLLNARTFLKEIAMDAGIGIRLDF
                     TYFVFRLDAALPLLDPARPEGSRWLLPKSRLSDLVWNFGIGYPF"
     gene            1348..>2165
                     /locus_tag="PK976_03620"
     CDS             1348..>2165
                     /locus_tag="PK976_03620"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HRC67008.1"
                     /translation="MTYTEAVNYIFQHLPMFTKIGGVAYKADLSRTLYLCEKLGNPHL
                     NFRSIHIAGTNGKGSVASFLSSIFMESGSKTALFTSPHLRDFRERITINGILIPGDYV
                     AEFIEENRPNFETIQPSFFEMSFALAVKWFSDQKVDVAIIETGMGGRLDSTNVILPEI
                     SIITNIGLDHQQFLGSTLAEIAAEKAGIIKPNIPVVIGEHNQQTDRVFIEKAIQTHSL
                     LFWAEDLSSVEDSFYDVENNYLNLSLMILSGKLIQLQSPLPAEYEIKNIRTVMAA"
BASE COUNT          616 a          450 c          436 g          663 t
ORIGIN      
        1 atacagctgg tatttccctg acgcgacatg gccatctcga ttgttatatc tacttgtcgc
       61 gagctgaagt acagggattc actatcgaaa cagaagttac caatacggga ggtgatctgg
      121 gattgtcggg taatttggtt tataccaatc gaaatgtttt tcgaggagcc gagatgctaa
      181 ggctaaaact gagaggtgca gccgaaatgc aaaaatcgtt cgagcaaact tcacagaata
      241 agcttcttct gttcaatact tatgaatcag gagccgaact caatctgact ttcccgcgtt
      301 ttgtttggcc tttgaaagga ttacgttttc ccagttccat gcgaactaag tcgaatcttc
      361 tggttggata caattatcaa aaccgacccg attatattcg ccatattact aatgctgcct
      421 tttcttatca attgaaagta tccgatttta agagtcatct tttaacaccc ttagaattga
      481 acgctataaa aatatatgca actccttcat tccagcaact catcgattcg atcgacgatc
      541 aaggcattcg gagtcaatat agtgatcatc tgataccagc cttgagatac agctatattt
      601 ttaccaatca ggaaattggt aaattccaca attacaaata tttccgtttc gacttcgaat
      661 catcgggcaa taccctttac gctgtggatc gattaaccaa tgtatcaccg aatattcaca
      721 acgaatatac cttcttggga attgcctatg cacaatattt gaaagccagt tccgattttc
      781 ggtattttta tatgtttaac ttgaataata ccttggttat gcgaattttt gccggggctg
      841 ctattcccta tggtaatagt aattatcttc ccttcgaaaa gggcttcttt gctggcgggg
      901 caaatggcat gaggggatgg acgcttcgaa tgctgggacc tggccatttt tcaagcgaag
      961 gtggtcaaag atataaccgc atgggcgatc tccatttcga aaccaatctc gaatatcgtt
     1021 tccctatgta taatttcttt aaaggtgcat tctttctgga tgcaggaaat atttggctgg
     1081 taaaatccaa tccaatgtat ccgggaggcc tgttaaatgc cagaactttt ctcaaggaaa
     1141 ttgccatgga tgctggtata ggtatccgtt tagattttac ttatttcgtt tttcgtttgg
     1201 acgctgcgtt gcctcttctc gatcctgcaa ggccagaggg atcacgatgg ctactgccaa
     1261 aatctcgttt atcggatctg gtatggaatt ttgggatagg ttatcctttc taaatgcacg
     1321 tgttctgtta aaacgattat tgaatacatg acttatactg aagcggtaaa ttatattttt
     1381 caacatttgc ctatgtttac aaaaatagga ggtgttgcct ataaagccga tttgagccgc
     1441 actctttatt tgtgtgaaaa gctcggtaat cctcatctaa actttcgttc cattcatatt
     1501 gcaggtacaa atggaaaagg cagcgtggcc tcttttctaa gttccatctt catggagtcg
     1561 ggttcgaaaa cagctttgtt tacctcccct catctgcgcg attttagaga aagaattacc
     1621 ataaatggaa tactcatccc cggagattat gtagctgaat tcatcgaaga aaacagacca
     1681 aatttcgaaa ccattcaacc ttcatttttt gaaatgagtt ttgctcttgc tgtcaaatgg
     1741 tttagcgacc aaaaggtaga tgtggccata atagaaaccg gaatgggagg caggctcgac
     1801 tcaaccaacg taatccttcc tgagataagc atcataacca atattggact agaccatcag
     1861 caatttttag gctcgacact tgctgagata gctgccgaaa aggccggaat catcaaacca
     1921 aacattcccg tggtgatagg cgagcataat cagcaaaccg atcgtgtttt catcgaaaaa
     1981 gctatccaaa ctcattcatt actcttttgg gctgaagatt taagttctgt cgaagattcc
     2041 ttctatgatg ttgagaataa ttacttgaac ctttcgctga tgatcctttc gggaaaactt
     2101 atccagctgc aaagccctct tccggctgag tacgaaatta aaaacataag aaccgtgatg
     2161 gccgc
//
LOCUS       DAORPD010000236         2157 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_9328_length_2157_cov_2.857279, whole genome shotgun sequence.
ACCESSION   DAORPD010000236 DAORPD010000000
VERSION     DAORPD010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9328_length_2157_cov_2.857279"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="PK976_03625"
     CDS             complement(<1..181)
                     /locus_tag="PK976_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67009.1"
                     /translation="MFMIMGCHREFSSGEEPARWVDPFIGTAGHGHTYPGATLPFGMV
                     QLSPQTRLEGWDGCSG"
     gene            complement(210..>2157)
                     /locus_tag="PK976_03630"
     CDS             complement(210..>2157)
                     /locus_tag="PK976_03630"
                     /inference="COORDINATES: protein motif:HMM:NF012906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67010.1"
                     /translation="LVAYNGIKVEDASITTQSIVHDTALLKCEITVFSHFDKEISTKF
                     ELYFHGNLISNKDLNIEKGSHSFEFDFPVVHPQLWWCNGLGKPYLYEFELSLKTEKKE
                     WFHKNIRVGIRNIQLVLQPDSIGTSFYFKLNGVPVFMKGANYIPSHSFLTETTNSDYE
                     RLLRNAKEANMNMLRVWGGGVYERDEFYSLADEMGILIWQDFMFACAMYPGDEEFFQN
                     VRQEAIEQVRRLRNHPCLALWCGNNEIDEAWHNWGWRNQYQNSKLRDAVWSHYLNLFH
                     RLLPAVVHQYDPGTSYIPSSPRFGWGRSQSMVSGDSHYWGVWWGQQPFEILLQKIPRF
                     MSEFGFQSMPEISTIRKVQPPEADSLFSPQLRCHQKCSLGFEILSVYLEREGFSPQTL
                     EEYIYTTQLVQAKGIGLGLAAQRASRPHCMGSLFWQLNDCWPVVSWSSMDFQGNRKAL
                     FYKTRQLFDPIAIETFIHEQEVKIFLFSDLPKDIKGTLTFEMRYYTGKLVPILQAEVN
                     VSANSSSCVYSVPLNEFQQVGDYLFVATLRFDDSIIRKAYCLPGLLSQQNLPEAHLLT
                     SIRRENDGYIISVKSDVFIAFIQFYLKESPAEFSDNFIHVWPGQNVEIFCRTSLPEQE
                     FQRQFSFFTLNELYRINLNRHENK"
BASE COUNT          666 a          448 c          432 g          611 t
ORIGIN      
        1 atcccgaaca accatcccat ccttcgagac gggtttgagg gctaagttgc accattccaa
       61 aaggaagcgt agcacccgga taagtatgcc cgtggcctgc agttcctatg aaaggatcta
      121 cccatcgggc gggttcttct ccggatgaga attctctgtg gcatcccata atcatgaaca
      181 ttatgggaat gagccaaacg acaaaaaatc tacttgtttt catgtctatt caaattaatt
      241 cgataaagtt cattgagtgt aaaaaagctg aattggcgct ggaattcttg ttccggcagg
      301 ctggttctgc aaaatatttc cacgttttgg cctggccata catgaatgaa attgtcagaa
      361 aattcggccg gagattcttt cagataaaac tgtatgaatg caataaagac atcacttttc
      421 acggaaatga tataaccatc gttttccctg cgaatcgatg ttagtaaatg tgcttcggga
      481 aggttttgct ggcttaataa acctggcaag caatatgcct ttcgaatgat agaatcgtca
      541 aaacgtaaag tggctacaaa taaataatct cctacttgtt gaaattcgtt caatggtaca
      601 gaataaacac aagacgaaga atttgcggaa acatttactt cggcttgcaa aattggaaca
      661 agctttcccg tgtaatatct catttcgaaa gtcaatgtac ctttaatatc tttcggtaaa
      721 tcggagaata ggaagatttt cacttcctgt tcatggatga aagtttcaat agcaattggg
      781 tcgaaaagct ggcgtgtttt ataaaacagg gcttttcgat tgccttgaaa atccatggat
      841 gaccacgaaa ctaccggcca gcagtcgtta agctgccaaa aaagcgaacc catgcaatgc
      901 gggcgtgagg ctctttgggc agccaatccc agtcctatac ctttagcctg aacaagttgg
      961 gttgtataga tatattcttc taatgtttga ggcgaaaaac cctcacgttc gagataaacc
     1021 gataggattt cgaatcctaa tgaacatttt tgatgacaac gcaattgtgg agaaaacaaa
     1081 gaatcagcct cgggaggttg aacttttctg atggtagata tttcgggcat agattgaaaa
     1141 ccaaactcac tcataaaacg aggaattttc tgcaatagaa tttcgaaagg ttgctggccc
     1201 caccaaacgc cccaataatg ggaatcaccc gataccatgc tttgcgaacg accccagcca
     1261 aatctcggag aagatggaat ataggacgtt ccagggtcgt actgatgaac cacagcaggt
     1321 aataagcgat gaaataaatt gagataatga ctccagacag catctcgaag tttgctattt
     1381 tggtattggt ttcgccagcc ccaattatgc caggcttcgt ctatttcatt gtttccacac
     1441 catagagcca ggcaaggatg atttcgaagc cgtcttacct gctcgatggc ttcttgtctt
     1501 acattttgaa aaaattcttc atcgccagga tacatcgcac aggcaaacat aaagtcctgc
     1561 cagataagaa tacccatctc gtcggcaaga gaataaaatt catctctttc gtaaactcct
     1621 ccaccccaaa ctcttaacat gttcatgtta gcttcttttg cattacgcaa taatctttca
     1681 taatcagaat ttgtagtttc ggtaagaaaa ctatgcgatg gaatgtaatt ggcacctttc
     1741 ataaatacag gaacgccatt cagtttgaaa tagaacgaag tccctatgct gtcaggctgt
     1801 agaacaagct gaatgttccg aatcccaacc cgaatatttt tatgaaacca ttctttcttc
     1861 tctgttttca aagaaagttc gaattcatac aaatagggct tacctaatcc attacaccac
     1921 caaagctgtg gatgaacgac cggaaaatcg aattcgaatg aatggctgcc tttttctatg
     1981 ttcaagtcct tattcgaaat caaatttcca tgaaagtaaa gttcgaattt ggttgagatt
     2041 tctttgtcga aatgagaaaa gacggttatt tcacatttta gcaaagcagt gtcgtgaaca
     2101 atagattgag tagttataga ggcatcttct acttttattc cgttgtaggc caccaaa
//
LOCUS       DAORPD010000237         2156 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_9332_length_2156_cov_2.017135, whole genome shotgun sequence.
ACCESSION   DAORPD010000237 DAORPD010000000
VERSION     DAORPD010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9332_length_2156_cov_2.017135"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            430..1113
                     /locus_tag="PK976_03635"
     CDS             430..1113
                     /locus_tag="PK976_03635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HRC67011.1"
                     /translation="MSKTLLRMTSLSKKVFVSLAGLFLITFLLLHLSINLFILPITPN
                     HEETFRQAVHFMTTSPLIKVMEVVLFAAFIIHIFFAVTLQIENWIARPRGYKVWQKSR
                     TDSPFSKLAIYTGIIVLIFLIIHFMNFYFVKQGWVSPPEGAYEVADKHDFYNMALNLF
                     NNPVYCVLYIVLIIVLGFHLHHAFQAGFQSLGLNHPIYTPIIKILGWIYSILVPLGFI
                     SIPLYFLIS"
     gene            1132..>2156
                     /locus_tag="PK976_03640"
     CDS             1132..>2156
                     /locus_tag="PK976_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005633282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRC67012.1"
                     /translation="MTQLNAKIPQGSLQEKWTNYKANQKLVNPANKRKFEIIVVGTGL
                     AGASAASALGELGFNVKVFCIQDSPRRAHSISAQGGINAAKNYPNDGDSIWRLFYDTI
                     KGGDFRSREANVYRLAEVSNNIIDQCVAQGVPFARDYAGYLDNRSFGGAQVSRTFYAR
                     GQTGQQLLLGAYGALSKEIKRGSVKMYTRHEMLDLVIVDGIARGIITRDTITGKIERF
                     AAHAVIIATGGYGNVYFLSTNALNCNASAIWNCYKKGAFFANPAFVQIHPTCIPVHGD
                     YQSKLTLMSESLRNDGRIWVPKKKEDVEAIRSKKFRAVDIPENDRDYFLERRYPAFGN
                     LVPRDVAS"
BASE COUNT          610 a          394 c          443 g          709 t
ORIGIN      
        1 acagactaag gcttgctgag gcaaaagatt tattaatttc ttccgattcc gttggaataa
       61 attgggataa acaatgttgg tgtcgttcat tgtaaaaatt tttaaaagaa gggattatac
      121 atttatttac tcgatggatg aattcaaagg aaaatattat ttttgagatg caaccttaga
      181 agtatagcat tttgaaagca ttcttgaaat ttttcggtag agaggattgt tttttccatt
      241 aaaatagttt ttaaatttgt tagttcaatc atcgaattta ttcaattatg aatatttatc
      301 atctcagccg ttaattatca tgcgaagata aagaaaaaag cctttaatta atgtcttcta
      361 taaaagaaag ggcaaatgac tgtattcgaa ttaaataaat attttatatt cactcaaacc
      421 ttataattta tgagtaaaac tttacttaga atgacatctt tgtcgaaaaa ggtttttgtt
      481 tcgctggcag ggcttttctt gattactttt ttgcttctgc atctgagcat caaccttttt
      541 attttaccca ttacgcccaa tcatgaggaa acattcaggc aggcggttca tttcatgact
      601 acttctccgt tgattaaggt gatggaagtg gtactttttg cagcttttat cattcacata
      661 ttttttgccg taactctgca gatagagaac tggattgcac gaccaagggg atataaggtc
      721 tggcaaaagt ccagaactga ttcacctttt tctaaattgg ccatatatac gggcatcatc
      781 gttctcatct ttctgatcat tcatttcatg aacttttatt ttgtgaagca gggttgggtg
      841 tctccgcccg aaggcgcata tgaggtagcc gacaagcatg atttttacaa tatggctctc
      901 aatcttttca ataatcctgt atattgcgtc ttatacatcg ttctgatcat cgtacttggg
      961 tttcatcttc atcatgcttt ccaggctggt tttcaaagcc ttggactgaa tcatcctatt
     1021 tatacgccca tcattaaaat attaggttgg atttattcca tcttagttcc attgggcttc
     1081 atcagcattc cactttattt tttgatttcg taattcaaat cattccatga tatgactcaa
     1141 ctcaatgcaa aaattccaca aggttcactt caggagaaat ggacgaatta caaggccaat
     1201 cagaagctgg taaatcctgc caataaaagg aaatttgaaa ttatagtagt tggcactgga
     1261 ttggccggtg cttctgctgc ctctgctttg ggggaactag gtttcaatgt aaaagtgttt
     1321 tgtatacaag atagtccaag gcgggcgcat agcatttcag cccagggtgg cataaatgct
     1381 gccaaaaatt atcctaatga tggagatagt atctggaggc tgttttacga taccatcaaa
     1441 ggaggagact ttcgttcacg tgaagccaat gtttatcgtt tggccgaagt aagcaacaat
     1501 atcatcgacc agtgtgtagc ccaaggagtt ccatttgcga gagattatgc tggatatctt
     1561 gataaccggt cttttggagg cgcccaggta tcacgaacct tttatgctcg tggacaaact
     1621 gggcagcaac ttcttttagg agcttatgga gccttgagca aggaaatcaa acgcggttct
     1681 gtcaaaatgt acacccggca cgaaatgctc gatctggtga ttgtggatgg catagcccgt
     1741 ggaattatta cccgtgatac gattactggc aagattgagc gttttgctgc acacgcagta
     1801 atcattgcaa ctggtggcta tggcaatgtt tattttttat ccaccaatgc tttgaattgc
     1861 aatgcaagtg caatatggaa ctgttacaag aaaggggctt tctttgccaa tccggctttt
     1921 gttcaaattc atcctacttg tattcctgtg catggtgatt atcagagtaa gcttacgctc
     1981 atgagtgaaa gtttgcgcaa tgatggacga atttgggttc ccaaaaagaa ggaagatgtt
     2041 gaagccatcc gcagtaagaa atttcgagcc gtggatatcc ccgaaaatga tagggactat
     2101 tttcttgaac gtcgctatcc tgcctttggt aaccttgtgc ctcgtgatgt agcttc
//
LOCUS       DAORPD010000238         2153 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_9352_length_2153_cov_2.177788, whole genome shotgun sequence.
ACCESSION   DAORPD010000238 DAORPD010000000
VERSION     DAORPD010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9352_length_2153_cov_2.177788"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1585)
                     /locus_tag="PK976_03645"
     CDS             complement(<1..1585)
                     /locus_tag="PK976_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67013.1"
                     /translation="MQSLPVEIKCSEVAANGDVTIYWQPGGSVIDFQRNEVYYSSDIF
                     SSFDLIAQIPDLGILHYRHIGANANAVHAYYFVRTITTTGLSFDSDTIAAILPVGSIL
                     DQTYIQLQWNALMDTLPSSCDGWYRIFCREEGGKWTKIDSTTDLSYIHEVRPCVDRYY
                     KIEVDDSYCGSVSAIAKVDHDIEQPPTPIFDSVSVVNGRIHLGWQEVNAPDAAGYIVY
                     LQDQGIWDTLALIPDAHTTFFIDTLNDPSVKSWKYSIAAFDYCGNVSEDMGISQAQGS
                     ILLAEPIFEVCLGRIRLHWSSYTNLPGGLGGYTVYLSENNNTFYPFVHLNASTLTYDF
                     IQPQDKVKYSFFIRANSINGQISASSNVRYLHVRIPPQPEYAYIRYGTVVNNQSIALG
                     FHVDTAAPIQKFFLQRMTNYSGNFAQVKEFTQKDAVIYFIDSAVNVSKNQYHYRLIVL
                     DSCGEETLVSNLASNILLKNPHSSDLQWNAYRDWYNGLEGYEVYRIEFLDTVLVAKLN
                     PDDTIWSDPNIDRETGAMYF"
     gene            1907..>2153
                     /locus_tag="PK976_03650"
     CDS             1907..>2153
                     /locus_tag="PK976_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HRC67014.1"
                     /translation="MTLIAPSILTTDFSKLSDTISMLQLSEADWIHLDIMDGVFVPNL
                     TFGFPVIRAIRKHTTKPLDVHLMMVEPEKYFKEFREAG"
BASE COUNT          691 a          445 c          388 g          629 t
ORIGIN      
        1 taaaatacat agcacctgtt tcacgatcga tatttggatc gctccatatt gtgtcgtcgg
       61 gatttagttt tgctaccaaa acagtgtcta aaaattctat cctgtaaact tcataccctt
      121 cgaggccatt ataccaatct ctatatgcat tccactgcaa atcggagctg tgcggatttt
      181 tcaacaatat gttcgaagct agatttgata ctaaggtctc ttcgccacaa ctatccaaaa
      241 caattaaacg ataatgatac tggtttttcg aaacatttac cgcagaatct ataaaatata
      301 taacagcatc tttttgcgta aactccttca cttgtgcaaa atttcccgaa taattggtca
      361 ttctttgaag aaagaatttt tgaataggag ccgcagtatc tacatgaaac ccgagggcaa
      421 ttgattgatt gttcaccaca gtgccataac gaatataagc gtattcgggt tgagggggta
      481 ttcttacatg taagtaacga acattactcg atgcggagat ttgtccgttg atcgaatttg
      541 ctctgataaa aaaagaatat tttaccttat cttgaggctg aataaagtca taagtcaagg
      601 tcgaagcatt tagatggacg aaaggataaa acgtgttgtt attttcgctc agatacaccg
      661 tatacccgcc gaggcctccc ggcaggttgg tataagaaga ccaatgaagc cggatgcgtc
      721 ctaagcatac ttcaaaaata ggctcagcca ataaaatcga tccctgggct tgggaaattc
      781 ccatgtcctc gctcacattg ccacaataat cgaaggctgc aatagaatat ttccatgatt
      841 ttactgaagg atcattcagc gtatcgataa aaaaagtggt atgagcatct ggaattaacg
      901 ccagtgtatc ccaaatgccc tgatcttgca aataaacaat gtaacccgct gcatcgggag
      961 cattcacctc ttgccagcct aaatgtatcc ggccattcac aacacttaca ctatcgaaaa
     1021 tgggtgtagg tggctgttcg atatcatgat caacctttgc aatagctgaa accgatccac
     1081 aataactatc atccacttca atcttgtaat acctgtccac gcagggacga acctcatgta
     1141 tataactcaa atcagttgta ctgtctattt ttgtccactt tccaccctct tcccggcaaa
     1201 aaattctgta ccacccatca caactcgatg gcaaagtatc catcaaagca ttccattgca
     1261 gttgaatata agtttggtcg aggatagaac caaccggtaa aatggctgca atcgtatccg
     1321 aatcaaacga aaggcccgta gtagtaatag tcctaacgaa ataataagca tgaacagcat
     1381 tggcatttgc tccaatatgt cgataatgca atattcccaa atccggaatc tgagcaatga
     1441 ggtcgaaaga agaaaaaata tcggatgaat agtaaacttc atttcgctga aaatcaataa
     1501 cactcccccc tggctgccaa taaatggtaa catcgccatt cgctgccact tcactgcatt
     1561 ttatttctac aggtaatgat tgcacctgtg aaaatggcat cgtatataaa aaaagaatga
     1621 aaagaataga aggaaaggtc attttcatga aacgaaaacg taaagttcgt gtcctatatt
     1681 tttgcaaatt taaagataca gcaaattatc ctttagtttc ctaccgaaat aaaacttcgg
     1741 aaaggtcgaa atgtagcatg aagcgagtgc aattgtccta tgcctgtgca acgaatctat
     1801 tattattcat tattcatcat ttaaaacaaa tcatttgaat cgaatttcga tgttatgcat
     1861 tctatttaaa aaatttttat gaatattgca ttctaacaat ctgcttatga ctttaattgc
     1921 accttctatt ctgactactg atttttccaa attatcggat acgatttcta tgttgcagct
     1981 tagcgaagcc gattggattc atctcgacat catggacggg gtatttgttc ccaatcttac
     2041 ttttggtttt ccagtaattc gtgcaattag gaaacacact accaagccat tggatgtgca
     2101 tttaatgatg gtagaacccg agaaatattt caaagaattt cgagaagccg ggg
//
LOCUS       DAORPD010000239         2149 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_9370_length_2149_cov_2.519102, whole genome shotgun sequence.
ACCESSION   DAORPD010000239 DAORPD010000000
VERSION     DAORPD010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9370_length_2149_cov_2.519102"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..903
                     /locus_tag="PK976_03655"
     CDS             <1..903
                     /locus_tag="PK976_03655"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF002187.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HRC67015.1"
                     /translation="GKLDRLMKVNIDPNSGFCFGVIKAVETAEEHLQRGHELLGLGEM
                     VHNEAELERLENLGLKTVSIEQFPDLKGKAVLLRAHGEPPSTYRIAAENSVQLIDATC
                     PIVKRLQEEMKKAWQSGKDQGVQMIIFGKPLHAEAIGLNGQVDNNGIIVENLDDLQTI
                     DARRPAHLFSQTTMDAGKFEKIAEKLREMYTETGQNLIIHKSFCRQVSNRMKSIKEFA
                     ASVDVLFFVASKNSSNGKVLYQTAQSINERTFFISGIQDIDFQKIGIDWGKVETIGIS
                     GATSTPPWLMHEIARLIENAFDLT"
     gene            923..2038
                     /gene="recF"
                     /locus_tag="PK976_03660"
     CDS             923..2038
                     /gene="recF"
                     /locus_tag="PK976_03660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HRC67016.1"
                     /translation="MWLRHLNLIQFKNFGELEVDFSPKINCFIGENGAGKTNILDAIY
                     YLSFTRSCFNSMDVQNILHGEEFFAIHGDYVMDSSLENKVHCIQKKGHAKEFSLDEKR
                     YERMADHIGLIPLVMLSPYDNDLIYDGSEARRKYIDGVISQFDHPYLDDLLIYNKALA
                     NRNLLLRQFADSGRFDPEMLEIYNPQLIGPGNRIFEKRQKFIDQFVPIFEKYYNLISA
                     EKEKISLHYVSQLAEKPMQTLLCENFDRDRLTRYTNGGIHKDDLEFIIENYPIKRFGS
                     QGQQKSFIVAIKLAQFEFTRELTGIKPILLFDDIFDKLDECRVETIVRLVGSENFGQV
                     FITDTQANRVYNIFIENPIHHKIFMVRNGELEEVDLI"
BASE COUNT          667 a          378 c          466 g          638 t
ORIGIN      
        1 gggaaactcg atagactaat gaaagtgaac attgatccca acagtggatt ttgttttgga
       61 gtaattaaag cggttgagac ggcggaggaa cacttgcaaa ggggtcatga attattaggc
      121 ctgggtgaaa tggtgcataa tgaggccgaa ttagagcgat tagagaattt gggtttaaag
      181 acggtttcta ttgaacagtt tcctgattta aaagggaagg cagtgctttt gcgtgctcat
      241 ggcgagcctc cttcgaccta tcgaattgcg gccgaaaata gtgttcagct catagatgct
      301 acttgtccaa tcgttaagcg acttcaggaa gaaatgaaga aagcatggca aagtgggaaa
      361 gatcaaggag tacaaatgat catctttggc aagcctttac atgcggaagc cataggattg
      421 aatggtcaag tcgataataa tgggattatt gtggaaaatc tcgacgattt gcaaactatt
      481 gatgcacgac gtccggcaca tttatttagc caaactacca tggatgcagg aaaattcgaa
      541 aaaatcgccg agaaattgag ggaaatgtat accgaaacgg gtcaaaatct tattatccat
      601 aaaagttttt gccgtcaggt aagtaacaga atgaagagta ttaaggagtt tgcagcttct
      661 gtggatgttt tgtttttcgt tgccagtaaa aatagttcaa atggcaaggt gctctatcaa
      721 actgcccaaa gtatcaacga acgaactttt tttatttctg gaattcaaga cattgatttt
      781 cagaaaatag gaattgattg gggaaaggta gagaccatag gcattagtgg agctacaagt
      841 acacccccat ggcttatgca cgaaattgca aggttaatcg agaatgcctt cgacctaact
      901 taaagtatcg tatttttgca gcatgtggct gaggcactta aatctgattc aatttaagaa
      961 ttttggtgag ctggaagttg atttttctcc aaagatcaat tgttttatag gagaaaatgg
     1021 agccggaaag accaatatcc tcgatgctat ttattatctt tcgtttactc gttcatgttt
     1081 caattcgatg gatgttcaaa atatcctcca tggtgaagaa ttttttgcaa tacatggaga
     1141 ttacgtgatg gatagtagcc ttgaaaataa agtacattgt attcagaaaa aagggcatgc
     1201 caaggagttc tcgctcgatg aaaaacgtta cgaacggatg gccgatcaca taggattgat
     1261 tcctctggtg atgctttctc cttatgataa tgatttgatt tacgatggaa gtgaggctcg
     1321 tcgtaaatac atcgatggag tgatctccca attcgaccat ccttatctcg atgatctgct
     1381 catttacaac aaagcgctcg ccaatcgtaa cttattgctt cgtcagtttg ccgattctgg
     1441 ccgtttcgat cccgaaatgc tcgaaatata caatcctcaa ttgattggcc cgggaaatcg
     1501 tattttcgaa aaacgccaga aattcatcga tcaatttgtc ccgattttcg aaaagtatta
     1561 caaccttata tcagccgaaa aggaaaaaat aagccttcac tatgtgagtc aacttgcaga
     1621 aaagcctatg caaacactgc tttgcgaaaa tttcgatcgc gaccgcctga ctcgttatac
     1681 caatggaggt attcataaag atgatctgga gttcattatc gaaaattatc ctatcaagag
     1741 atttggttca cagggtcaac aaaaatcttt cattgtagcc ataaagctag cacagttcga
     1801 atttaccaga gaattgaccg gcatcaagcc aattcttctg tttgatgaca tcttcgataa
     1861 gctcgatgaa tgcagggttg aaacaatagt tcgccttgta ggaagtgaaa actttggcca
     1921 ggttttcatt accgatacac aggcaaatcg agtttataat attttcatcg agaatcccat
     1981 ccaccataag atatttatgg ttagaaacgg agagctggaa gaagttgacc tgatttgaac
     2041 ttttagagtg tatttccctt tttgtggttt ttctatattc aatacttcag gatttttcct
     2101 gtaaattgtt ggtcatcaca ttgaatattt aagatgtaga aaccatttt
//
LOCUS       DAORPD010000240         2147 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_9382_length_2147_cov_2.125239, whole genome shotgun sequence.
ACCESSION   DAORPD010000240 DAORPD010000000
VERSION     DAORPD010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9382_length_2147_cov_2.125239"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1182)
                     /locus_tag="PK976_03665"
     CDS             complement(<1..1182)
                     /locus_tag="PK976_03665"
                     /inference="COORDINATES: protein motif:HMM:NF023855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme N-terminal
                     domain-containing protein"
                     /protein_id="HRC67017.1"
                     /translation="MSYIKFVKDQLINLEFSLAREILRSNRAGAYASQTIIGCNTRKY
                     HGLLICPQAGIDDDNHLLLSNLDESIVDEGSEFNLAIRRYKGGIYNPKGHKYAREYEI
                     DHVPIITYRIGNMVITRQLVFAMNEDRVLIKYTLVDGQSPTKIRFKPFLAFRNIHFLT
                     HANLNANTNAQRVPGGIMVKMYEGYTPLYMQFSKEAIEYLHVPDWYYDVEYLKEIQRG
                     YEAHEDLFTPGYFELNLKKGENVIFSAGTTEADISKLKAIFNLEVRRRLSRSNFLNCL
                     KNAAQQFIVHRDGKTQLIAGYPYFGRFSRDTFISLPGLTLTVGNSRLCHEVLQSHLSE
                     LKGPLFPNILSKEKPVYNSIDAPLWFIWAVQKYACKTRRISSVWKEFGPAIIQILEGY
                     RK"
     gene            1391..>2147
                     /locus_tag="PK976_03670"
     CDS             1391..>2147
                     /locus_tag="PK976_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67018.1"
                     /translation="MQRFQQKLRVISILTLLLPSLILLSQKKPLTHEVYEKWNSIPYK
                     CISPNGKFVAFTIQPARGDGFLHLFNVSDHQVKVFPRGVNPAFSDNNYVIFSVKQPYD
                     TLRKLRLKKIKENDLPLDSLFILSLTTDNLLRFPHLYNFKLMEESPLVCFKRKFTPSD
                     TSSSDSTKYHPPKKKTSYQLGIFESFDKPCWFFPYIEDYATPRLNGPVAFVQKLPEND
                     SSGLYIFNPLKGQTIKVFQKPGKIKNLSWNETGT"
BASE COUNT          653 a          448 c          370 g          676 t
ORIGIN      
        1 ttttctatat ccttcgagaa tttgtatgat ggccgggcca aattccttcc ataccgatga
       61 aatcctccga gttttgcagg catacttctg tactgcccaa ataaaccata aaggagcgtc
      121 gatggagtta tacactggtt tttctttgct caagatattt gggaaaagag gacccttgag
      181 ctctgacaaa tgagattgca gcacttcgtg gcataatctg gaattgccga ccgtcaatgt
      241 aagaccagga agtgaaatga aggtatctcg actaaatctt ccaaaataag gataacctgc
      301 tattaactga gttttgccgt cacggtgaac aataaactgc tgagctgcat ttttcaggca
      361 attcaaaaaa ttactgcgcg acaagcgtct gcgaacctcc aggttaaaaa tggctttcag
      421 cttcgaaatg tcggcttcgg tcgtccctgc cgaaaatatt acgttttctc ctttttttag
      481 gttcaattcg aaatagcctg gggtgaaaag atcttcgtgc gcttcatagc ctctttggat
      541 ctcttttaaa tattccacat cgtaatacca atcaggaaca tgaagatatt cgatcgcctc
      601 tttcgagaat tgcatatata aaggtgtata gccttcgtac atctttacca ttattcctcc
      661 gggcactctt tgagcgttag tattggcatt taaattggca tgggttagaa aatgaatatt
      721 ccgaaaggct aaaaagggtt taaaacgaat cttcgtaggc gactgcccat caacaagcgt
      781 atatttgatc aaaaccctgt cctcattcat ggcaaatacg agttgcctcg taatgaccat
      841 attgcctatt cgataggtaa ttatgggtac atggtcaatt tcatattctc gtgcatattt
      901 atggcctttg ggattgtata ttcctccctt gtatcttctt atcgccagat tgaattcgct
      961 tccttcatcg acgatacttt catcgagatt cgaaagcaac agatgattat catcgtctat
     1021 gccagcttgt ggacaaatga gcagaccatg atatttacgc gtattacacc ctatgatggt
     1081 ttgagaagca taagcaccgg cacgattcga tcgtaaaatc tcgcgtgcaa gtgaaaactc
     1141 aaggttaata agttgatctt taacaaattt aatataactc atacaatgaa tttctaaaag
     1201 tcataggcca ttttgagaat gcaaataaac atatttcaaa tgaaatataa attaactttc
     1261 gaaaacaatt ctaacagcaa atatgctaaa atttcctttt gcttccatgc attcttcgta
     1321 attttacacc ttctttctac aatttctgcg gtttgtttaa atttataaaa gtattaaaaa
     1381 aatccctgaa atgcaacgtt ttcaacagaa attgagggtt atatcgattt taactctcct
     1441 cttgccttcc ctcatcctct tatcccagaa aaaaccactt actcatgagg tttatgaaaa
     1501 atggaactcc attccgtata aatgcataag cccgaatgga aagtttgtag cttttaccat
     1561 acagcctgca agaggtgatg gcttccttca tttgttcaat gtttccgacc atcaggtaaa
     1621 agtttttccc agaggggtta atcccgcatt ttctgacaac aattacgtga tatttagcgt
     1681 taaacagcct tatgatactt tgagaaaatt gcgtttgaaa aagataaaag aaaatgactt
     1741 acctctcgat tccctcttca ttctttcact taccactgat aacctgctca ggtttcccca
     1801 tctctataat tttaaattga tggaagaaag ccctttggtt tgtttcaaaa gaaaatttac
     1861 cccctcagat acaagttctt ccgatagcac caaatatcat cctcctaaga aaaaaacatc
     1921 ttatcaactc ggtattttcg aatcattcga taaaccatgc tggtttttcc cttatattga
     1981 agattatgct acaccccgat taaacgggcc tgtggccttt gttcaaaagc tacccgagaa
     2041 tgattcttct ggcctttata tttttaatcc tttaaaaggc cagacaatca aagtttttca
     2101 aaagcccgga aagatcaaaa atctaagttg gaacgagaca ggaacat
//
LOCUS       DAORPD010000241         2147 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_9386_length_2147_cov_1.925908, whole genome shotgun sequence.
ACCESSION   DAORPD010000241 DAORPD010000000
VERSION     DAORPD010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9386_length_2147_cov_1.925908"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2147)
                     /locus_tag="PK976_03675"
     CDS             complement(<1..>2147)
                     /locus_tag="PK976_03675"
                     /inference="COORDINATES: protein motif:HMM:NF039534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67019.1"
                     /translation="TTTYWATYENQCGISTCDSLQINVTPQPLSPSALQVDTNFFCTG
                     TINQINLTATGGAGDTLYWFKDECGGNPIGYGTPLTISAPDSSAIYHARWGNKCNLSN
                     CLSIEVEVLQPPVRPFSLSTNENDFCAEEVGVIQLSASGGSGDTLQWFKGNCGGIYIG
                     TGSPLLISAPGDTTIYYSRWTNLCGQSECDSIIINVRPQPRPLDSLSVDTNYFCISYN
                     ENIQLSAYGIYEYGDTVRWYEGGCGQNFIGSGNSLTIAAPDVSTTYYANNNNSCGESI
                     CRSIQVIVNTPEPPVLLTANPLFKCVSDTGQVTLKSFGGNGEIISWYADSCGGVPIGH
                     GDSITISYPDTTTFFYARWENYCGESPCVSIPVNVVEIPSPPAQILSDTNYYCIGDVD
                     YITLTAFGGYGDTLSPWGETLRWFVKSCGGATIGTRKNITIPAPVASTWFFARWENAC
                     GVSICDSFNLIVYTPRPPDSVIADTNYFCPGTVDSVSLMAWGGVGDQILWAKIYGNDT
                     IAAGSTNPFRVAAPNTTTRYLVRMKNYCGISKWFPFDIVVNLPQPPKLIVSDLDSLVC
                     SNYPGYIHLWTIGGWGDTVKWYSTDASGQNPVFLYSGDTLSLSPPTGTSYLMATFVTA
                     CGESKGTKTHIRTVPAPIVNIISNDSICEGSRYHVMGDLISNFHHLKWTVDGIGNLDH
                     PTQKSPVYSYGNHDIINPTVVYLKVNVFGNYPCG"
BASE COUNT          635 a          448 c          471 g          593 t
ORIGIN      
        1 ccacatggat aatttccaaa aacattcacc ttcagataaa ccacggttgg atttataata
       61 tcgtgatttc catatgaata aactggagat ttctgtgttg gatggtcaag attaccaatg
      121 ccatccactg tccattttaa atggtggaag tttgatataa gatcacccat aacatgatag
      181 cggcttcctt cgcagatgga atcgttggat ataatattta cgatgggtgc aggtactgtt
      241 cgtatatgtg ttttagtacc tttactttcg ccgcatgctg ttacaaatgt tgccatcaag
      301 taggaggtac cagtgggagg tgaaagacta agagtgtctc cactgtatag aaatactgga
      361 ttttgtccag aggcatcggt ggaataccat ttgacggtat cgccccatcc accaatcgtc
      421 cataaatgaa tatatccagg ataattggaa caaaccagac tgtctaaatc actaacaatc
      481 agtttgggag gttgggggag gtttacgacg atatcgaaag gaaaccattt cgaaatgcca
      541 caataattct tcatacgaac cagatagcgc gtggtagtat taggagctgc tacacgaaat
      601 ggatttgtcg aacctgcggc tatggtatca ttcccatata tttttgccca taaaatttgg
      661 tcacctactc ctccccatgc cattaacgaa acactgtcca cagtgccagg gcagaagtaa
      721 tttgtatcgg caattacgga atcaggtggt cgaggagtat atacgattaa attgaaagaa
      781 tcacaaatag aaactccaca ggcattttcc cagcgagcaa aaaaccaagt tgaagccaca
      841 ggcgcaggaa ttgtgatatt ttttctcgta cctattgtag ctcctccaca ggacttgaca
      901 aaccagcgca atgtttcacc ccagggcgaa agagtgtcgc cataacctcc aaaagccgta
      961 agtgtgatat aatccacatc ccctatacaa tagtagttgg tatcggaaag aatttgagca
     1021 ggaggggagg gaatttcgac tacattcact ggaatgctca cgcaaggcga ttctccacag
     1081 taattttccc atcgggcata aaaaaatgta gttgtatcgg gatatgaaat agtgatcgaa
     1141 tcgccatggc caattggcac accaccacat gaatcggcat accatgaaat gatttcgcca
     1201 ttgcctccga atgatttcaa tgttacctgt ccggtgtctg aaacgcattt gaataaagga
     1261 tttgcagtaa gcagtacagg aggttcagga gtattgacta ttacttgaat cgagcggcat
     1321 atcgattctc cgcatgaatt gttgttgttg gcataatagg ttgtgctaac gtcaggtgca
     1381 gcaatggtaa gtgaattacc tgaaccaatg aaattttggc cgcaacctcc ttcataccat
     1441 cgaaccgtat ccccgtattc gtatattcca taagctgata gctggatgtt ttcattataa
     1501 gagatgcaga agtagtttgt atctacagat aatgaatcga gaggacgggg ttgtggtcta
     1561 acattgatga tgatagaatc acattcggat tgcccacaaa gattagtcca tctcgaataa
     1621 tatatcgtgg tgtcgcctgg agcgctaatc aacaatggag aacctgttcc aatataaatt
     1681 ccgccgcaat ttcctttgaa ccactgaaga gtgtctccag aacctccact tgccgaaagt
     1741 tgtattactc caacttcttc tgcacaaaaa tcgttttcat ttgtactcag gctaaatggc
     1801 cgaactggcg gctgcaacac ttcgacttca atcgagaggc aattgctcag gttgcatttg
     1861 ttcccccagc gagcatgata gattgcagaa gaatcggggg ctgaaatagt caaaggagtt
     1921 ccatatccta tcggattccc tccgcattca tccttaaacc aatataaggt atccccagct
     1981 cctcctgtag ctgtaaggtt gatctggttt atggttccag tacagaaaaa attagtatct
     2041 acttgtaggg cagaaggtga taaaggctga ggggttacat ttatctggag actatcacag
     2101 gtagagattc cgcattgatt ttcgtaagta gcccaatatg tggtggt
//
LOCUS       DAORPD010000242         2140 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_9425_length_2140_cov_3.010072, whole genome shotgun sequence.
ACCESSION   DAORPD010000242 DAORPD010000000
VERSION     DAORPD010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9425_length_2140_cov_3.010072"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1260
                     /locus_tag="PK976_03680"
     CDS             <1..1260
                     /locus_tag="PK976_03680"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HRC67020.1"
                     /translation="HHRPGDELPPASAVLDPKRSDCTYPYNELSGCGVGFKLIQAYSR
                     FKGWSSQDLVKYLDLVAVSIAADIVQITGENRILAYYGLKLINSNPRPGIEALLEISG
                     IKRTNEKPDRIFTRELNINDLVFIVGPRINAAGRIESGNNSVELLISQSLEEARTLAE
                     QINNCNTERRNLDSLITQQALDMIDEQETLSKAFTTVLYHPNWHKGVIGIVASRLTEI
                     YYRPTVILCKSNGLITGSARSIKDFDIYDAIDACSDLLEHFGGHTYAAGLSLKPENLP
                     RFTSKFEEYAREKFNGNHPAPEIEIDQVINLSDITPSFFNILKQFAPFGPGNMSPIFA
                     THKLMDTGYSRIVGKNHLKLSVVHPHISGYPVSGIAFGLSKYFEDIQKGQPFSICYHI
                     EENEWNGKTNLQLNVKDIKFEPLDHEF"
     gene            complement(1555..1628)
                     /locus_tag="PK976_03685"
     tRNA            complement(1555..1628)
                     /locus_tag="PK976_03685"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1592..1594),aa:Pro,seq:cgg)
     gene            complement(1675..1748)
                     /locus_tag="PK976_03690"
     tRNA            complement(1675..1748)
                     /locus_tag="PK976_03690"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1712..1714),aa:Ile,seq:cat)
     gene            1847..>2140
                     /locus_tag="PK976_03695"
     CDS             1847..>2140
                     /locus_tag="PK976_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67021.1"
                     /translation="MNPVNNIIFDFGGVILNIDYKRSLNAFLKLGIQWEDSALEGFNR
                     SDAYIGLELGNICPENFFDELRKYFPESVSNKQLKDAWNAILLDMPPERVELLK"
BASE COUNT          659 a          439 c          423 g          619 t
ORIGIN      
        1 catcaccgcc ccggagatga gcttcctccg gcgagtgccg tgctcgatcc caagcgctcc
       61 gactgtactt atccctataa tgaactctct ggatgtggag taggctttaa acttatacaa
      121 gcatatagtc gttttaaagg atggtcatcc caagatctgg taaagtacct cgatttggtt
      181 gccgtgagca ttgcggcaga tattgtgcaa attaccggag aaaacaggat actagcctat
      241 tatggattga aactaatcaa ttcaaatcct cgtcccggta tcgaagcttt attggaaata
      301 agtggaatta aacggaccaa cgaaaaaccc gacaggattt ttaccagaga attgaatata
      361 aacgatctgg tatttattgt ggggccacgc ataaatgctg ccgggcgaat cgaaagcgga
      421 aacaatagtg tagaacttct tatctctcaa tcattagaag aagcccgaac acttgccgaa
      481 caaatcaaca attgcaatac agaacggcgt aacctcgaca gcctcatcac acagcaagct
      541 ctggatatga ttgacgaaca ggagactctg tcaaaagcat ttacgaccgt gttgtatcat
      601 cccaactggc ataagggggt gattggaatt gtggcttcgc gtcttaccga aatctattat
      661 cgacctacgg ttattctttg caaatcgaat ggattaatta ccggttcggc tcggtctatt
      721 aaagattttg atatttatga tgccattgat gcttgcagcg atttgctcga acattttgga
      781 ggtcatacgt atgctgctgg acttagctta aaacccgaaa atcttcctcg ctttacttca
      841 aaattcgaag aatatgcgag agaaaaattc aatggcaacc atccagcacc cgaaatcgaa
      901 atcgaccagg ttatcaactt aagcgatatt acgccttctt tttttaatat tctgaaacag
      961 tttgctcctt ttggccctgg aaacatgtca cccatttttg ccacgcataa actaatggat
     1021 acgggctatt cgcgtattgt tgggaaaaat catctcaaat tatctgtggt tcatcctcat
     1081 atcagcggat accctgtttc gggaattgct ttcggtttgt cgaaatattt cgaagatata
     1141 caaaaaggac aaccttttag tatatgctat catatcgaag aaaatgaatg gaacggaaaa
     1201 acaaacttgc agcttaatgt aaaagacatt aaattcgagc ctctggatca tgagttttaa
     1261 tattggccag aactcaaccc tataccaaat tcttctttca tccttataca tttttggttc
     1321 ttttaagttt tgatttcctt tgcagttgct catagcatct tcgactggga aacctaaaca
     1381 gaatatttta ttggttttga acacatttga cgatattgct catttcattt atgaaacaaa
     1441 tcttaccatc tatcattatc ctggaaaata gaaaagcatg caggcaaatt cctgcatgct
     1501 ttaatagata ggcagaagaa ataatgaaaa agacctcagc gatgagaggc cttgtcggga
     1561 tgactggact cgaaccagcg gcctccacgt cccgaacgtg gcgcgctaac caactgcgct
     1621 acatcccggg ataaagaatg tattcgtaca aaacaagctt aaatcgctgg attgtgcgcc
     1681 cacctggact tgaaccaggg gcctactgat tatgagtcag ttgctctaac caactgagct
     1741 atgggcgcaa ttttttttaa taaattttga aaacccgagt gcaaatttac atctttgctc
     1801 aaaaatatca acatgaactt tgataaatat ttaaaatgta ttgaaaatga accctgttaa
     1861 caacattatc ttcgatttcg gaggtgtaat cttaaacatt gattataagc gttctttgaa
     1921 tgcatttctg aagctaggaa ttcaatggga agactctgct ctggaaggat ttaatcgtag
     1981 cgacgcttat attgggctcg aacttgggaa tatatgccct gaaaattttt tcgatgaact
     2041 gagaaaatac tttcctgaat ctgttagcaa caaacaactc aaagacgcat ggaatgccat
     2101 cttgctggat atgcctcccg aaagagttga attattgaaa
//
LOCUS       DAORPD010000243         2136 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_9451_length_2136_cov_2.353196, whole genome shotgun sequence.
ACCESSION   DAORPD010000243 DAORPD010000000
VERSION     DAORPD010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9451_length_2136_cov_2.353196"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..905)
                     /gene="ftsA"
                     /locus_tag="PK976_03700"
     CDS             complement(<1..905)
                     /gene="ftsA"
                     /locus_tag="PK976_03700"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HRC67022.1"
                     /translation="METKERIADLGEIVDPSDIYVTSLDIGTTKIAAIVGRRNENNKV
                     EILGYGRSDSLGVRRGVVTNIENTVQSIQNAVRQAEERSGKKIKYVHVGIAGHHIKSM
                     QHRGTLIRKNGEDEITQEDIENLINNMYSLNMNPGEEIIDVIPQEFTVDGESGIKNPV
                     GVLGTSLEANFHIITGQTAAAKNIYKCVKKAGLEIVDLIIEPFASSEAVLSPEEKEAG
                     VVLVDVGGGTTDLAIFQDDIIRHTAVIPFGGEIVTEDVKEGCSIIRKHAEELKIKFGS
                     SLASENRDEDVVSIPGLRGRPPKEIT"
     gene            complement(895..1695)
                     /locus_tag="PK976_03705"
     CDS             complement(895..1695)
                     /locus_tag="PK976_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67023.1"
                     /translation="MKIKKLLEFTGWAVAVAGTSFLLSFANSEQNKTVCKDIIIQLHQ
                     GDYALVSENEILGKLTKNYGNLQGKPIHKIPFRNIESEIMKISALSRADVFATPSGYV
                     KISAVERMPLIKIFNHQNKNFYLDKEGNIFTTQTSHSARVLIANGNISLEPRAGTNIY
                     HFPDSVIGIKQVRAIAYVAQHISNNKTLNALISQIFVNHRNEIELIPLIANHVIILGD
                     TSNLTEKFENLLIFYKRMGNLNQLNRYETINLKYKNQIVCTKNTIYGN"
     gene            complement(1692..>2136)
                     /locus_tag="PK976_03710"
     CDS             complement(1692..>2136)
                     /locus_tag="PK976_03710"
                     /inference="COORDINATES: protein motif:HMM:NF014874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HRC67024.1"
                     /translation="EGIRRRFDILYQSDNFTLIDDYAHHPEEIKALAISLRNLFPTKK
                     LTAIFQPHLYSRTRDLAQGFAQSLSLFDEVFLMDIYPAREHPIHGVDSQMIMKKMSQV
                     AHLVTREEAFREIISRKHQVLATIGAGDIEKLALLAKENLNRQKS"
BASE COUNT          547 a          445 c          438 g          706 t
ORIGIN      
        1 gtaatttctt tgggtgggcg gcctcttaat ccaggaatcg agaccacatc ttcgtctcgg
       61 ttttcgctgg ccaaagacga tccgaatttt atcttcagtt cctcggcatg tttacgaatg
      121 atggaacaac cctcctttac gtcttcggtt acaatttctc ctccaaaggg gattacggcc
      181 gtgtggcgga taatatcgtc ttgaaagata gccaagtcgg tagttcctcc gcccacatcg
      241 accagaacta cccctgcttc tttttcttcg ggggataaaa ccgcttctga agatgcaaaa
      301 ggttcgatga tgagatctac gatttcgagc ccggcttttt ttacgcattt gtaaatattc
      361 ttggcagcgg cggtttgtcc ggtgattatg tgaaaattgg cttcgagcga agttcctaaa
      421 actcctacgg gatttttgat accactttca ccatccacgg taaattcttg aggaattaca
      481 tcaataatct cctcgccagg gttcatgttc agactgtaca tgttattaat cagattctca
      541 atatcttcct gagtaatctc gtcttcacca ttctttcgaa tgagtgtacc tcgatgctgc
      601 atgctcttga tatgatgccc ggcaatgcct acatgaacat acttgatttt cttgcccgat
      661 cgctcctcgg cctgccttac agcattctga atggattgaa cagtattctc gatattggtg
      721 accactcctc gtcttacccc caaagaatcg gaacgcccgt agcccaatat ctcgactttg
      781 ttgttctcgt ttcgacgccc gacaatggcc gcaatcttgg tggtgccaat atcgagactg
      841 gttacataaa tatcggaggg atcaacgatt tcccccagat cggctattct ctccttagtt
      901 tccatatatt gtgtttttag tacatacaat ttgattctta tattttaaat ttattgtctc
      961 gtaacggtta agctggttca aatttcccat ccgtttgtag aagatcaata gattctcgaa
     1021 tttctcggtt aaattcgatg tatctcccaa aataataaca tgattggcaa taaggggaat
     1081 gagttcgatt tcgtttctat ggttcacgaa tatttggctt atgagcgcat tcaaggtttt
     1141 attattgctg atatgctgtg ctacataggc tatagctctt acctgtttta tacctataac
     1201 tgaatcgggg aagtggtaga tattggttcc ggctcttggt tccaaagaga tattgccgtt
     1261 tgctatcaat actctggctg aatgtgaggt ttgcgtggta aagatgttcc cttccttgtc
     1321 taaataaaaa ttcttatttt ggtggttgaa aatttttatg agcggcattc tttctacggc
     1381 tgaaattttt acatagcccg aaggagttgc aaaaacatca gccctcgata aagcggatat
     1441 tttcattatt tctgattcta tgtttcggaa tggaatttta tggataggct ttccttgtaa
     1501 attgccgtaa tttttggtaa gttttcctaa gatttcgttt tcagaaacga gcgcataatc
     1561 gccttgatga agctgaataa tgatatcctt gcatacggtt ttattttgct cggaattagc
     1621 gaatgaaagc aagaaggaag ttccagccac tgccaccgcc catccggtaa attcaaggag
     1681 tttcttaatc ttcatgattt ttgtcggtta agattttctt tggcaagcaa ggctaatttt
     1741 tcgatatcac ctgcaccaat tgttgccaac acctgatgtt tgcggcttat gatttctctg
     1801 aatgcttctt cacgggtaac taaatgtgcc acttggctca tttttttcat aatcatctgg
     1861 gaatctacac catgaatagg gtgttcacgt gcaggataaa tatccatgag aaatacctca
     1921 tcgaaaagag acagactttg tgcaaaacct tgagctagat ctctggtacg actatagaga
     1981 tgaggttgga aaatggcagt taattttttc gtgggaaata aattccgcaa agatatggcc
     2041 aatgccttta tttcttcggg atgatgcgca tagtcatcaa tcagggtgaa attatccgac
     2101 tgataaagta tatcgaaacg acgacgaatt ccttcg
//
LOCUS       DAORPD010000244         2134 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_9466_length_2134_cov_2.229437, whole genome shotgun sequence.
ACCESSION   DAORPD010000244 DAORPD010000000
VERSION     DAORPD010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9466_length_2134_cov_2.229437"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="PK976_03715"
     CDS             complement(<1..620)
                     /locus_tag="PK976_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HRC67025.1"
                     /translation="MGLFSFLSREIAMDLGTANTIIIHNDRVVVDEPSIVAIERSTGK
                     VIAVGKRAQMMHGKTHENIKTIRPLRDGVIADFQAAEIMMREMIRMIPENHSIFPQSL
                     RMVISIPSGITEVEERAVKDSAEQAGAKEIRLIHEPMAAAIGIGIDVLEPTGNMIVDI
                     GGGTCEIAVIALGGIVTNESIHVAGDDFNDDIEEYMKKQHNINIGER"
     gene            complement(636..>2134)
                     /gene="purH"
                     /locus_tag="PK976_03720"
     CDS             complement(636..>2134)
                     /gene="purH"
                     /locus_tag="PK976_03720"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005640988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HRC67026.1"
                     /translation="LISAFHKEILLPWAETLFSHGVELLATDGSYRFLKDKGFEVQSI
                     ESLTGFPSIFSGRLKTLHPTIMGGILYQRDHPDDLNEAKLYGIPAIDLVIVDLYPFEQ
                     TVASTNHQEEIIENIDIGGISLIRAAAKNFKSVAVVADNASMNEALEWYRKGDGSFTE
                     EQRMILATRAFAVSSRYDAAILSYFSNQTGNHYLRITEDHHKILRYGENPHQKGIYFG
                     RSSSYFAQLHGKEISYNNLLDIDTALSLISEFTEPTIAIIKHGNSCGIASHAELMTAL
                     EMTLRGDPVSAFGGVIVSNRTIDKETVVKINSLFFEVILAPGFENEALQMLKSRKNRV
                     ILQTTGKSLPDKLYRSAAGGFLMQDRDSFPDDPRQWECKTRVEPTPEQTEDLLFANKV
                     VKHLKSNAIALVKNKQLIGMGAGQTSRVDALRYAIEKSHNFGFDLEGAVMASDAFFPF
                     ADSVELAHKAGITAIIQPGGSIKDQESIDYCDQHNMAMVFTGIRHFKH"
BASE COUNT          567 a          475 c          442 g          650 t
ORIGIN      
        1 cgttccccta tgttgatatt atgctgtttc ttcatgtact cttctatatc gtcgttaaaa
       61 tcatctccgg ctacgtgaat agattcattg gttacgattc caccaagggc tataacagca
      121 atttcacaag taccacctcc aatatcaaca atcatgtttc cggtaggttc tagaacgtcg
      181 ataccaattc caatagcagc ggccataggt tcatgaatca gacggatttc ttttgcacct
      241 gcctgctcgg ctgaatcttt taccgcacgt tcttcaactt cggtaatacc cgaaggtatc
      301 gaaatgacca ttctgagtga ttgaggaaag atactatggt tttcgggtat cattcgtatc
      361 atttcacgca tcatgatttc ggcggcctga aaatcggcaa ttactccatc gcgtaatgga
      421 cgtatggttt taatgttttc atgggttttc ccatgcatca tttgggctcg cttgccaacg
      481 gcaatcacct tgcccgtaga gcgttcaatg gctacaatgc ttggttcgtc tactactact
      541 cgatcgttat gtataatgat ggtattggcc gtacccaaat ccattgcgat ttccctgctt
      601 aaaaatgaaa aaaggcccat aaaacgattt atatgttaat gtttaaaatg tcgaatgcct
      661 gtaaatacca tggccatatt atgctggtca cagtaatcaa tgctctcctg gtctttaata
      721 gaacccccgg gctggataat ggcagtgatc cctgctttat gagccaattc aactgaatcg
      781 gcgaaaggga aaaaggcatc ggaggccatc actgctccct caaggtcgaa gccaaaatta
      841 tggctctttt ctatagcata cctcagagca tctactctcg aagtttgccc ggctcccata
      901 ccgataagtt gtttgttttt tacaagggct atggcattcg acttcaaatg ctttactacc
      961 ttgttggcaa acaacaggtc ttctgtttgt tccggagtag gctcgactcg agtcttgcat
     1021 tcccattgtc gaggatcgtc ggggaagcta tctctgtctt gcattaaaaa acctcctgca
     1081 gccgaacggt atagcttatc gggtaaactt ttgccggtag tttgcaaaat tactctgttt
     1141 ttacgagatt tcaacatctg gagagcctca ttttcaaatc ccggagcaag aataacttca
     1201 aaaaacaagg aattaatttt taccactgtt tctttatcta tggtcctatt gctgacaatc
     1261 acaccaccaa aggccgatac tggatctccc cgaagtgtca tttccaaagc ggtcattaat
     1321 tctgcatgag aagctattcc gcatgaatta ccgtgtttga taatagcaat ggtgggttcg
     1381 gtgaattcag atatcagact taatgccgta tctatatcga gtaaattgtt gtaagaaatt
     1441 tcttttccgt gaagttgagc aaaatacgaa ctcgatctgc caaagtaaat ccccttttga
     1501 tgtgggtttt caccataacg caatattttg tgatgatctt ctgtaattct gaggtagtgg
     1561 ttcccggtct gattgctgaa ataggatagg atagccgcat catagcgtga gctgactgca
     1621 aaggcacgtg tggcaagtat catgcgttgc tcctcagtaa aagagccatc cccttttcga
     1681 taccattcga gggcttcatt catgcttgcg ttatcggcca ctacggctac agatttaaaa
     1741 ttcttggcag ccgctcgaat tagcgaaatg cccccaatat caatattttc gataatttct
     1801 tcctgatgat ttgtgcttgc tacggtttgt tcaaagggat acaaatccac tattacaagg
     1861 tcaatggcag gaataccata taatttggct tcgttcaggt cgtcgggatg atcacgctga
     1921 tatagtatgc ctcccattat cgtgggatgc agcgtcttga gcctgccact aaaaatggaa
     1981 gggaatcctg ttaaagattc tatactttga acttcaaacc ctttatcttt caagaagcga
     2041 tatgatccat ccgttgccag aagctcgact ccatgggaaa agagagtttc ggcccacgga
     2101 aggagaattt ctttgtggaa tgccgaaatt aagg
//
LOCUS       DAORPD010000245         2134 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_9467_length_2134_cov_2.103415, whole genome shotgun sequence.
ACCESSION   DAORPD010000245 DAORPD010000000
VERSION     DAORPD010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9467_length_2134_cov_2.103415"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="PK976_03725"
     CDS             <1..231
                     /locus_tag="PK976_03725"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336385.1"
                     /note="catalyzes the phosphorylation of 2-butanoate to
                     butanoyl phosphate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HRC67027.1"
                     /translation="QVAKEIGAMASVLKGNVDAIILTGGIAHNTYVVNYIKEMTGFIA
                     PIVVYPGEDEMSALAINGLMVLRNEVNAGIYE"
     gene            388..1113
                     /locus_tag="PK976_03730"
     CDS             388..1113
                     /locus_tag="PK976_03730"
                     /inference="COORDINATES: protein motif:HMM:NF014607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein O N-terminal
                     domain-containing protein"
                     /protein_id="HRC67028.1"
                     /translation="MRTPAILLHYIHYTDHSIIARLYTRQGGIKAFIIKGINKKNPSL
                     KPSQLLPLSLLEIVYEEKPHNRIFFLKEARNYFLSYKVQNHVIKAGITLFMAEILNHS
                     IKEEEANILLYDFLEKTIIRLETQEENVAFYPIWFLIRLSSFLGITPLDNYSSETITF
                     NMEEGKFQENVPERNTYVAGENVKTLSLLIKAFSSSDYFIPNIHIKDKTAIIDILIRY
                     YHQHLPGFGEIHTHKILKEVLNS"
     gene            1400..>2134
                     /locus_tag="PK976_03735"
     CDS             1400..>2134
                     /locus_tag="PK976_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009037059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HRC67029.1"
                     /translation="MTKARVLFVSQEMVPYLKDSPMGQISRYLPQGIQEKGKEIRTFM
                     PRYGHINERRNQLHEVIRLSGINIIIDNTDRPLIIKVASIQQARMQVYFIDNDDYFAR
                     KFQFYDKNDKFFKDNDERAVFFARGVLETVKKLGWNPDIIHCHGWFTSLLPLYVKTAY
                     KDNPIYTDAKIIVSLYDDDFEGEMGKTIKSKIKLPGIREKHLANYKKGTYIGFMQGAL
                     DFADGAIIASENVNPEILSYLDQKNIP"
BASE COUNT          713 a          424 c          400 g          597 t
ORIGIN      
        1 caggtagcta aggaaatcgg agctatggca agcgtgttga agggaaatgt cgatgctatt
       61 attcttacag gaggaatagc tcataatacc tatgtggtaa attacatcaa ggaaatgact
      121 ggcttcatcg ctcccattgt ggtttatccc ggagaagatg aaatgagtgc cctggccata
      181 aacggcctca tggtattaag gaatgaagta aatgctggca tttatgaata ggtggtggaa
      241 aaaaccatct gttatccctt aaattgccca aggtttgcta tttgaatcct acaacagaaa
      301 attcggcctg cctatcatga tcaaaaacag tcgaaaagag gtaattttgc atcataacct
      361 caaatctgct gcatcattcc taatattatt cgcacaccgg caatattact ccactacatt
      421 cattataccg atcatagcat tattgccagg ctttatactc gccagggagg tataaaagca
      481 ttcataatca aaggcatcaa caagaagaac ccttcactga aaccttcaca gcttttgcct
      541 ctgagcctcc ttgaaattgt atacgaagaa aaaccgcaca atcgtatttt cttcctgaaa
      601 gaagcgagga attacttttt aagttacaag gtacaaaatc atgtgatcaa ggcgggtatt
      661 acactcttca tggccgaaat tctaaaccac agcatcaaag aagaagaagc caacatcttg
      721 ctgtatgatt ttctcgaaaa aaccatcatt aggttagaaa cccaggaaga aaatgttgca
      781 ttctacccta tctggtttct catcaggctt tcgagttttc tggggattac tcctctggat
      841 aactactcct ccgaaactat aaccttcaat atggaagagg gaaagtttca ggaaaatgtc
      901 ccagaacgaa atacttatgt ggcgggtgaa aatgtcaaaa cgctttctct tctcattaaa
      961 gcctttagct catccgacta ctttataccc aatattcaca tcaaagataa aacagccatc
     1021 atagacattc tcattcgata ctaccatcag catttacctg gctttgggga aattcataca
     1081 cacaagatct tgaaagaagt cttaaattca tgaaattttc actgtcgaat cgaatcagaa
     1141 aatcaagaat tatcgccaaa catgaaaaaa tacaagcccc cctcgatata gtatgatgca
     1201 gagtcaaatg tgggaaatcc tttgcatctc gagaactatt cctgcacaca tgattcatga
     1261 ggggttttgt attgctaaca gtgaggtgtt tcgaaaaaaa ttttatatag aattagtaat
     1321 caatcgattt tttctctatt taaggaaaaa atcgtaactt tgcaaagaaa agacgactaa
     1381 tttttataat tttttcataa tgactaaagc tcgtgtgctc tttgtttcgc aagaaatggt
     1441 tccttattta aaggacagtc ctatgggaca aatttcaaga tatctgcccc agggtattca
     1501 ggaaaaggga aaagaaatca gaacattcat gccaagatat gggcatatca atgagaggcg
     1561 taatcagctt catgaagtaa tcagattatc gggcattaat attatcatcg ataataccga
     1621 tcggcctctg attatcaagg tggcttctat tcagcaagca cgcatgcagg tttattttat
     1681 cgataatgat gactattttg cccgaaagtt tcagttttac gataagaacg acaaattttt
     1741 caaggataac gacgaaagag cggtattctt tgcaagagga gtgctggaaa ctgtcaaaaa
     1801 acttggctgg aatcccgata ttatccactg ccatggatgg tttacaagtt tactcccact
     1861 ttatgtgaaa actgcctata aagacaatcc tatctacacc gatgcaaaaa tcatcgtatc
     1921 tctctatgat gatgattttg aaggcgaaat ggggaaaacc atcaaatcga aaataaagct
     1981 ccccgggatc agagaaaagc atctcgccaa ctacaagaag ggcacttata ttggatttat
     2041 gcagggagcc ctcgattttg ccgatggggc tattattgca agcgaaaatg taaaccccga
     2101 aatcttgagc tatctcgatc aaaaaaatat accc
//
LOCUS       DAORPD010000246         2131 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_9488_length_2131_cov_2.598266, whole genome shotgun sequence.
ACCESSION   DAORPD010000246 DAORPD010000000
VERSION     DAORPD010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9488_length_2131_cov_2.598266"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..954
                     /locus_tag="PK976_03740"
     CDS             <1..954
                     /locus_tag="PK976_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67030.1"
                     /translation="KKFKIPPAITAVLVLILLVRTTLVYPYPIRPYAEEKVIAEAAEW
                     IKENGLANKKLYYYHNLFALFLNKDPMNDDEGKMMIHFTSIKPDQIPVGALLAWDAHF
                     GPNEGGTPLENILTDSSFKLLKAFEPETPFKVLGGHDFKVYLFEKTSQTQFPVFFDTN
                     SGQIFFMRLGFEGHDAFDKWHSQEESFEGEFSLKMESSDEFTEILNTSCSSIPFQDSL
                     AIIEIKAWLKETSSNSINTTRLVIHATKDNHQLYLPLFPDTLSIQKDNWKPIQGKVSL
                     KKFLPDGHLKIYFWKTNPNETAFIDNVSIILPKNEDKIHVE"
     gene            1084..1461
                     /locus_tag="PK976_03745"
     CDS             1084..1461
                     /locus_tag="PK976_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007558396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRC67031.1"
                     /translation="MKRIISSKEAPKAIGPYSQANEANGFLFISGQIPIDSSRGQLVE
                     GGIKEQTHQVMRNIGAILKEASLDYKDVVKCTCLLSNMEHFGEMNEVYAQYFITDPPA
                     RAAYGVVKLPLGVLVEIECIAAR"
     gene            complement(1530..>2131)
                     /locus_tag="PK976_03750"
     CDS             complement(1530..>2131)
                     /locus_tag="PK976_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67032.1"
                     /translation="WLRLGVAYHSPMFFTDMNDVWDSYIESYFDKEVPNKSVGSPLGE
                     FEYELITPMKVIGSVAFQVGKIGLISADYQFVDYRDARFHPTGEFADINDIIQSSYTT
                     QNIIRVGTEWRFGPFSVRGGYGFYSSPFNNNINDGERKSGSLGVGYRSDNFFVDLGFV
                     QTKSSEKYFLYDPMNGDPLPAANTDFTGNSLLMTLGFKF"
BASE COUNT          670 a          425 c          402 g          634 t
ORIGIN      
        1 aaaaaattta aaattcctcc tgctattaca gcggttttag tattaattct cttagtgcgc
       61 accacattgg tttatcctta tcctattcgt ccctatgccg aagaaaaagt aattgccgaa
      121 gctgcagaat ggataaaaga aaatggcttg gccaacaaga aattatatta ttatcacaat
      181 ttatttgcgt tgtttcttaa caaagatccc atgaatgatg atgaagggaa aatgatgata
      241 catttcactt ccatcaaacc cgatcaaatc cctgtaggag cattgcttgc ttgggatgcc
      301 cattttggcc ctaacgaagg aggcactccc ttggaaaata ttcttaccga ttcctctttt
      361 aaactcctga aagcgttcga acccgaaaca cctttcaaag tattgggcgg ccatgatttc
      421 aaggtatatc tttttgaaaa aacttcacaa acacaatttc ccgtgttttt cgataccaat
      481 agtggccaaa tattttttat gcggctcggc ttcgaaggtc atgacgcctt tgataaatgg
      541 cattcccaag aagaatcctt cgaaggtgaa ttttctttga agatggaatc ttccgatgaa
      601 tttacagaaa tattaaatac ctcttgtagc tccattccct ttcaggattc gcttgccata
      661 atcgaaataa aagcatggtt gaaagaaact tcttcgaatt caataaatac aacccgcttg
      721 gttattcatg ctaccaaaga caaccaccag ctttatctac cgcttttccc tgatacttta
      781 tccatacaaa aagacaactg gaagccaatt caagggaaag ttagcctgaa aaaattccta
      841 cccgacgggc atttaaagat ttatttttgg aaaactaatc ctaatgaaac tgcatttatt
      901 gataatgtta gcatcatctt gcctaaaaat gaagataaaa tccatgtaga atgattgaag
      961 atttaaattt cgattcgcct tttcctaaaa tcagattatg tttttatttt tatgcgtttc
     1021 attagcttta ttaaaattca tcatcatatt ttcacaaata caccaataaa ttttatatag
     1081 acaatgaaaa ggataatttc atctaaagaa gcgcccaaag ctataggtcc ttatagtcag
     1141 gccaacgaag cgaatggctt cctatttatt tcaggtcaaa tacctattga ttcttccagg
     1201 ggacagttag tagaaggagg gattaaagaa caaactcatc aggtcatgag aaacataggc
     1261 gccattttaa aagaggcgag cttggattat aaggatgtgg taaaatgtac ttgtctttta
     1321 agcaacatgg aacattttgg ggagatgaac gaagtttacg ctcaatattt tataaccgat
     1381 cctccagcca gagcggctta tggagtagtt aagctgccct taggagtgct cgtcgagatt
     1441 gaatgtattg ctgcccgctg atcaaaaaga aaagctgtat cttcatttat ggcagataca
     1501 gcttctgata gatgaaccaa aaacctgatt tagaacttga atccgagggt catcaggagg
     1561 ctattccctg taaagtcagt atttgctgct ggtaaggggt ctccattcat aggatcgtag
     1621 agaaagtatt tttcggaaga tttggtctgt acaaagccaa ggtcaacgaa gaagttatct
     1681 gatcggtaac caactcccaa agaacctgat tttctttctc cgtcgttgat gttattgttg
     1741 aatgggctac tataaaagcc atagcctcct cttacgctaa agggtccaaa acgccattcc
     1801 gttcccactc taattatatt ttgggtagtg tagctagatt ggataatatc gttgatatca
     1861 gcaaattcgc cggtgggatg gaagcgagca tcccgataat cgacgaattg gtaatcggca
     1921 ctaatcaatc cgatcttacc cacctgaaat gcaaccgatc caatcacttt catgggtgta
     1981 atgagctcgt attcgaattc gcccaaggga gatcctaccg atttattggg aacctcttta
     2041 tcgaaataag attcaatgta gctatcccat acgtcgttca tatcggtaaa gaacatgggt
     2101 gaatgataag ccacaccgag gcgcagccaa t
//
LOCUS       DAORPD010000247         2130 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_9495_length_2130_cov_2.567711, whole genome shotgun sequence.
ACCESSION   DAORPD010000247 DAORPD010000000
VERSION     DAORPD010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9495_length_2130_cov_2.567711"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            46..>2130
                     /locus_tag="PK976_03755"
     CDS             46..>2130
                     /locus_tag="PK976_03755"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HRC67033.1"
                     /translation="MGVNPWIAIIGALAYGFSSYFIIILEAGHNTKAHAIAYMAPLLG
                     SILLTMKGKWLKGGILTAIFACLELYANHLQITYYFLILVLIVWIAFLIKSIREKQLV
                     SLGKATGVLIIAAFLAILPNFTQLWTTWEYSKETTRGKTELSHQKEIQTRGLDKEYVT
                     QWSYGVAETWSLMIPNVKGGETEQLGKSEFALEKIEPNMRRAIAQQNHYWGEQPFTSG
                     PVYVGAIIVFLFILGIFIVEGTLKWALVVATLLSIMLAWGKNFMPLTNFFLDYVPLYD
                     KFRAVSMILVIAELCMPLLGILALWKIYQYPSLAKNEASSFRKKFFIAYCLTAGISLI
                     FYMFPNLFFNFISSSERSQLNELRTQVADPSQIAVFIQSLETARIAIFRHDAIRSFLF
                     ITIAAGFIYAYTSKLRFPRPLFLISMGLLIFIDMYVINKRYVTEDKFESSRIVDSPFT
                     PTPADQLILKDTDIHYRVLNLTTNTFNDARTSYFHKSIGGYHGAKLKRYQELIDHQIS
                     KNNMEVLNMLNAKYFIVPDENRKPSVRINMEALGNAWFPKEYKLVNNADEEMEALNHF
                     KAAETAIIDKRFAQYLEEYQSLPDSTAIIQLKEYAPNRLVYHSITNTDRLAVFSEIYY
                     PYGWQAYVDDKPQPHFRANYVLRAMIVPAGEHEIIFKFEPKSYYLGEKISLAGSLLLL
                     IMLIGMIGAEIKK"
BASE COUNT          650 a          439 c          392 g          649 t
ORIGIN      
        1 tatgtttttc tctatttcat cggatttttc tttctcttgg ttgtgatggg agtcaaccca
       61 tggatagcca ttataggggc attagcttat ggtttttcat catatttcat cattatttta
      121 gaagccgggc acaatacgaa agcgcatgcc attgcttata tggcgccact cttaggaagt
      181 atcttgctta ccatgaaagg caaatggctc aaaggaggaa ttttaacagc cattttcgca
      241 tgtttggaac tttacgccaa ccacttgcaa atcacttatt attttctcat acttgtttta
      301 attgtttgga ttgcattttt gataaaatct atcagagaaa aacaattggt ttcattgggt
      361 aaggcaacag gagttcttat tatagctgca tttttagcta ttttacccaa tttcactcaa
      421 ttatggacga catgggaata tagcaaggaa accaccagag gaaagacaga gttgagtcat
      481 caaaaagaaa ttcaaacccg aggtctcgat aaggaatatg ttacgcagtg gagttatggg
      541 gttgcagaga catggtcgtt gatgataccc aatgtaaaag gaggagaaac cgaacaactt
      601 gggaaaagcg agtttgctct cgaaaagata gaacccaaca tgagaagagc cattgcacag
      661 cagaatcact attggggaga acaaccattt acttcaggtc cagtttatgt aggggctatc
      721 atcgtatttc tattcatttt aggaattttt attgtggaag gaacactaaa atgggcatta
      781 gtggtggcaa ccttactttc aataatgtta gcctggggta aaaactttat gccacttacc
      841 aatttcttcc tcgattatgt tccactttac gataagtttc gggcagtaag catgatactc
      901 gttattgctg agttatgcat gcccttgctc ggaattcttg cattgtggaa aatataccaa
      961 tatccttctc ttgccaaaaa tgaagcctca tcgtttcgta aaaaattttt tatagcctat
     1021 tgtttaacag caggcatctc gctaatattc tatatgttcc ctaatctttt ctttaatttc
     1081 atctcttcat ccgaaagaag ccagttgaat gaacttcgta cacaagtagc cgatccttca
     1141 caaatcgcag tattcattca atctctcgag acagcccgca tagctatttt cagacatgat
     1201 gccatacgca gcttcctttt cattaccata gcagcaggat ttatttatgc ctatacttct
     1261 aaattgcgat tccctcgtcc tcttttcctt atttcaatgg gcttactcat tttcattgat
     1321 atgtatgtaa tcaataagcg ttatgtaacc gaagataagt tcgaaagttc acgcatagta
     1381 gattccccct ttacccctac ccctgccgat caactcatcc tgaaagatac cgacattcat
     1441 taccgagtac tgaatcttac aaccaacacg ttcaatgatg cacgaacctc ttatttccat
     1501 aaatccatag gtggatatca tggagcaaaa ttaaaacggt atcaggagct tatcgatcat
     1561 cagataagca aaaacaacat ggaagttctc aatatgctta atgcaaaata ctttatcgtc
     1621 cctgatgaaa atcgcaaacc aagcgttcga atcaacatgg aagcacttgg aaatgcttgg
     1681 ttccccaaag aatacaaatt agtaaataat gccgatgaag aaatggaagc gcttaaccat
     1741 ttcaaggctg ccgaaactgc catcatcgat aaacgttttg cacaatatct cgaagaatac
     1801 caatctttac ccgattccac ggccataatc caactcaaag aatatgcacc caatcggctg
     1861 gtttatcatt caataacaaa taccgatcgc ttagcggttt tcagcgaaat atattatcct
     1921 tatggctggc aggcttatgt ggacgataaa cctcagccgc actttcgggc taattatgtt
     1981 ctgagagcca tgattgttcc tgcaggagaa catgaaatca tcttcaagtt cgaacccaaa
     2041 tcgtattatc tgggagaaaa aatatctctt gcgggctcgc tgcttctttt gataatgtta
     2101 ataggtatga taggggccga aattaaaaag
//
LOCUS       DAORPD010000248         2130 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_9496_length_2130_cov_1.848193, whole genome shotgun sequence.
ACCESSION   DAORPD010000248 DAORPD010000000
VERSION     DAORPD010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9496_length_2130_cov_1.848193"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..342)
                     /locus_tag="PK976_03760"
     CDS             complement(88..342)
                     /locus_tag="PK976_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67034.1"
                     /translation="MLHNKIHRYQNLFSGNILDIGARSKPYRNLFTEVAQYVGTNTCR
                     HHQINHLRPDEDTTDVWIGDGTALPFQNATFDGVVFKSSL"
     gene            complement(449..1342)
                     /gene="folD"
                     /locus_tag="PK976_03765"
     CDS             complement(449..1342)
                     /gene="folD"
                     /locus_tag="PK976_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HRC67035.1"
                     /translation="MKLLDGKTLFANIKGEIKTEVSQIIDHNERPPHLAAVLVGDDPA
                     SLSYVASKEKACRQVGITSSLYHYPENFTENELLQVIDFLNHDQDIDGFIVQLPLPPQ
                     IDPNKIILSISPDKDVDGFHPYNLGRMMMGQPNFIPATPLGIMQMLERYQIETKGKHC
                     VVLGRSMIVGTPVSILLSRKAKPGNATVTLCHSYTQNLKEICLTADILIIAIGKPRFI
                     TADMVKEGSVVIDVGIHRIPDNTKEKGYMIVGDVDFEEVSKKAEWITPVPGGVGNMTI
                     AGLLMNTLNAWKQRNIRQNIN"
     gene            complement(1422..>2130)
                     /locus_tag="PK976_03770"
     CDS             complement(1422..>2130)
                     /locus_tag="PK976_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HRC67036.1"
                     /translation="QAKPDQVLLVVDAMTGQDAVNTAKAFNEVIDYDGVVLTKLDGDT
                     RGGAALTVRAVVDKPIKFVGLGEKMDAIDVFYPERMADRILGMGDIVSLVEKAQEQFD
                     AEEAKKLQRKIAKDEFNFDDFLKQIHQIKKMGNVKDLMGMIPGMGKMVKDLDVDDDAF
                     KSIEAIIHSMTPEERQNPAIINGSRRKRIAMGSGTDVTEVNRLMKQFDETRKMMKMVT
                     TGGPRQMLKNMKQQKKR"
BASE COUNT          596 a          455 c          411 g          668 t
ORIGIN      
        1 gataaagata atcggatgac atcatgaaat atccttgcgg tttgagtact ctatttactt
       61 cctgaaaaaa tttagcagga tgagtaatca aagggaagac ttgaaaacaa caccatcgaa
      121 agtggcattt tggaatggga gtgcagtccc atctccaatc caaacatcgg tcgtatcttc
      181 atcgggtcga agatggttga tttgatgatg tctgcaggta ttggttccaa catattgtgc
      241 aacttccgta aaaagatttc tatagggttt tgatcgtgct ccaatatcta agatgtttcc
      301 actaaagaga ttttgatagc gatgaatttt attgtgcaac atccagatat ttattttgca
      361 tggattacat gaaaccactc cttcagaagg tttgccaaaa agatttgaac atgtacaaga
      421 gatgttcatc gagaaataaa caattcttct aattgatatt ctgcctgata tttctttgtt
      481 tccacgcatt tagtgtattc atcaataaac cggcaatggt catattcccg actccacccg
      541 gcacaggcgt aatccattca gcttttttgc ttacctcttc gaaatcgaca tcaccaacaa
      601 tcatatagcc cttttctttg gtattgtcgg gaatgcgatg aattccaaca tctattacta
      661 cacttccttc cttcaccata tcggcagtaa taaaacgagg tttacctatg gcaataatga
      721 gtatatcggc cgtaagacaa atttctttca aattctgagt gtaagaatgg caaagtgtaa
      781 ccgtagcatt acctggtttt gccttacggg aaagtaaaat agagacagga gtaccgacaa
      841 tcatgctacg tcccaacacc acacaatgtt ttcctttggt ttctatctga taacgctcga
      901 gcatttgcat gatacccaaa ggcgtagcag gtataaaatt aggttgtccc atcatcattc
      961 tccctaaatt ataaggatga aatccatcca catccttatc aggagaaatg gaaagaataa
     1021 tcttattagg atcgatttga ggtggaagag gtaattgcac gataaatcca tcaatatctt
     1081 ggtcgtggtt caaaaaatca atcacctgaa gcaactcatt ttcagtaaaa ttttctggat
     1141 aatgatagag ggaagaagta atgccaacct ggcgacaagc cttttcctta gaggcaacat
     1201 acgaaaggct ggccggatcg tctcccacca aaacggctgc cagatgaggc ggacgttcat
     1261 tatggtcaat tatttgggat acttcagttt ttatttctcc cttaatattt gcaaataagg
     1321 ttttgccatc gaggagtttc atcatgttcg aatatttata acaaagctaa cgaaaatttg
     1381 gtgaattgtt gaattcgaat tattggggaa tgttcatctg ctcaacgttt tttctgttgt
     1441 ttcatattct tgagcatttg acgaggaccg cccgtggtca ccatcttcat cattttacgg
     1501 gtttcgtcga attgtttcat tagcctgtta acttcggtca catcggttcc cgagcccatg
     1561 gcaattcgtt tacggcggct tccgttgatg atggcgggat tttgcctttc ttctggtgtc
     1621 atcgaatgaa taatagcttc tatacttttg aaagcatcat cgtcaacatc gaggtctttc
     1681 accatttttc ccataccagg tatcattccc atgaggtctt ttacattccc cattttttta
     1741 atttgatgaa tttgtttgag gaaatcatcg aaattaaatt cgtctttggc aattttacgt
     1801 tgaagttttt tggcttcttc agcgtcgaat tgttcctgag ccttttcgac gagtgaaacg
     1861 atatcgccca tgccaagaat acggtcggcc atacgctcgg gatagaacac atcaatggca
     1921 tccatttttt caccaagtcc aacaaattta atgggtttat ccaccacagc cctaacggtt
     1981 aatgccgcac cccctcgggt atccccatcc agtttggtaa gcacaactcc atcataatcg
     2041 atcacctcat tgaatgcttt agcagtgttt acggcatcct ggcctgtcat ggcatccacc
     2101 accagcagta cctggtccgg ctttgcctgg
//
LOCUS       DAORPD010000249         2129 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_9502_length_2129_cov_2.175024, whole genome shotgun sequence.
ACCESSION   DAORPD010000249 DAORPD010000000
VERSION     DAORPD010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9502_length_2129_cov_2.175024"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="PK976_03775"
     CDS             <1..443
                     /locus_tag="PK976_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HRC67037.1"
                     /translation="YMRKKHNIMIGERTAERVKIEVGAAITDLENPPADYAVHGRDML
                     TGIPKEIKVNYVEIAQCLDKSISKIEAAVLHALEMTPPELSADIYRTGIYLAGGGGML
                     RGLDKRIIQKTKLPVHVAEDPLRAVARGSGIALKNFDKFTFLIK"
     gene            483..1328
                     /gene="mreC"
                     /locus_tag="PK976_03780"
     CDS             483..1328
                     /gene="mreC"
                     /locus_tag="PK976_03780"
                     /inference="COORDINATES: protein motif:HMM:NF010532.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HRC67038.1"
                     /translation="MNNILRFIARYHFFFLFLLLEIISFLLIFSFNYYPQAVYFKLAT
                     SFNGKVREINASIANFYKLKSTNRQLAIENSILRSSLPSSFLAVDTNVFWHHDTLYRQ
                     RYKYITAHVIGNSLGKRNNYIMIDKGSREGVGIDMGVIGPQGVVGIVVTVSDHFASIM
                     PVLHSATMISAKLKKNNELTSVIWKGGNPFKASLINLPGQVQISKEDSVVTSGYSLIF
                     PEGILIGTIDHFHLNTDDNYYDADILLSTRFGSLSYVYVVQNLLRDEQLQLLKKQDEI
                     ISEEN"
     gene            1475..>2129
                     /locus_tag="PK976_03785"
     CDS             1475..>2129
                     /locus_tag="PK976_03785"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009138127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HRC67039.1"
                     /translation="MTKKVFLTFFLACGFILGSYACTNYIITKGASVDGSVMISYSAD
                     SHVLYGELYHWDAATWPAGTMLEVYEWDTGKYLGKIEQALQTYNVIGNMNEYQVSIGE
                     TTYGGREELWHQPGSIIDYGNMIYIALQRSRTAREAIKVMVELSEKYGYVSEGESFSV
                     ADAEEAWILEVIGKGKEIRIDASGLRKESKEFQRTLKPFVDKVFYSDHDFLKKLEEIA
                     "
BASE COUNT          660 a          410 c          440 g          619 t
ORIGIN      
        1 gatatatgag gaaaaaacac aatataatga taggggaacg tactgccgaa agagtgaaaa
       61 ttgaagtggg agctgcgatc accgatttgg aaaatccccc tgccgactat gcggttcatg
      121 gccgcgacat gcttaccgga atccctaagg aaataaaagt aaattatgtc gaaattgccc
      181 aatgcctcga taaatcaatt agtaaaattg aggctgccgt tctccatgca ctcgaaatga
      241 cacctcctga gctttcggca gatatctatc gtacaggtat ctatctggcg ggtggcggag
      301 ggatgcttcg aggcctcgac aaaagaatca tacaaaaaac caaattaccc gttcatgtag
      361 ccgaagatcc tctgcgcgcc gtcgcccgag gctcaggaat agctctcaag aatttcgaca
      421 agtttacatt ccttattaaa taatattagg agaaatattt ttacgaaacc ggccgaaaga
      481 ttatgaataa catcctgagg tttatagctc gatatcattt ctttttcctc tttttattac
      541 tcgagataat atccttttta ctcatatttt ctttcaatta ttatcctcag gctgtttatt
      601 tcaaactagc tacttctttt aatggaaaag ttcgagaaat caatgcttcc attgccaatt
      661 tctataaatt aaaatctaca aaccgacagc ttgccatcga aaactccatc ctgcgcagct
      721 cccttccttc gagctttctg gctgtcgata ccaacgtctt ctggcatcat gatacccttt
      781 acaggcaaag atacaagtac atcacagccc atgtcatcgg taacagcctt ggcaaacgca
      841 acaattacat catgattgac aagggaagta gagagggagt tggcatcgac atgggggtca
      901 taggacctca aggagtagta ggaattgttg tgactgtttc cgaccatttt gcttctatta
      961 tgcctgtgct tcacagtgct actatgatta gcgcaaaact gaaaaaaaat aatgaactta
     1021 cttcggttat ctggaaagga gggaatccat tcaaagccag cctgatcaac ctaccgggac
     1081 aagttcaaat ttcaaaagaa gacagcgtgg ttactagcgg ctattctttg attttccccg
     1141 aaggtatcct tatcggaacc attgatcatt ttcatctgaa taccgacgat aattattatg
     1201 atgccgatat attactgagt acgagattcg gcagtctttc ctatgtttat gtggttcaaa
     1261 atcttctgag agacgaacaa ctgcaactgc tgaaaaaaca agatgaaatt atttctgaag
     1321 aaaattaatc tcattggtca attatttaat tatcagcaat ttatttgaca acagggcttt
     1381 atttgctcaa ggggtggggg gtatgtaaaa aataatctat aattttaggc catcaaacta
     1441 accttttctt tatttaattt ataaattgat ttttatgact aaaaaagtat ttcttacatt
     1501 ctttttagcc tgtggtttta ttttgggcag ttatgcatgt accaattata tcattaccaa
     1561 aggcgcatca gttgatggaa gtgtaatgat tagttattcg gccgattcgc atgttcttta
     1621 tggagaacta tatcattggg atgctgctac ctggcctgca ggaaccatgc ttgaggtata
     1681 cgaatgggat acgggcaagt atctgggtaa aattgaacaa gctcttcaaa cctacaatgt
     1741 gatcgggaat atgaacgaat atcaggtttc tattggtgaa accacttacg gaggccgtga
     1801 agaactgtgg catcagccgg gttctataat agattatggc aacatgatct atatagcttt
     1861 gcaacgatct cgcacggcgc gtgaagctat aaaagtcatg gtcgaactat ccgaaaaata
     1921 tggttacgtg agcgaaggtg aatcattctc ggttgccgac gcggaggaag catggatatt
     1981 agaagttatt ggaaaaggga aagaaattcg catcgatgcc agtggcttac gcaaagaaag
     2041 caaggaattt cagagaaccc taaaaccctt cgtggataaa gtattttata gtgaccatga
     2101 ttttctgaaa aaattggaag aaatagctg
//
LOCUS       DAORPD010000250         2125 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_9529_length_2125_cov_2.414010, whole genome shotgun sequence.
ACCESSION   DAORPD010000250 DAORPD010000000
VERSION     DAORPD010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9529_length_2125_cov_2.414010"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            265..>2125
                     /locus_tag="PK976_03790"
     CDS             265..>2125
                     /locus_tag="PK976_03790"
                     /inference="COORDINATES: protein motif:HMM:NF025702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HRC67040.1"
                     /translation="MSLDSIDIYVNKTLMLDNVKFREASEFIQGPSGIPLLIEICPGN
                     SINSESAFYSTAVMFSQGKTYIAIAQGIIDGNGYHPWQPFELQIIDYGRETSNTPGQT
                     DVLFFGGSTDAPAFDIIETTSLGLLSDDLTYGQFDGYKELTPANYVLEIKDSTSGTRY
                     FRLQFDLTTYSGQAFVLMASGFLVPGLNNNGENLGFYIVSANGGMGVSLPQIPDPMAS
                     IQIVHLCADRALDSMDVWIGNKKFGADLPFGNSLPFIEILAEKNQVIAIQPAGSTSPD
                     NPLVSLSYTFHDRQKYILMLTGITSETGYTPAPALQIYPFDSARIEANSPNATDMLVF
                     HGSTDAPTIELYETGIGLGLLVENLEYGNFASEGYLSLTTADYVIEVRNKPGNEVLYT
                     YSLPLSALGLQGEALSLCVAGFVNPTNNSNGPAFGLYLSGKNGGALFELPIYIPPTTS
                     FVQFIHNIADTSAQSLDIWMNNQMLLDNHLFQKASPYLEVPANQNLTFSVLPQTSISP
                     DNPLTSLNLTLESGKHYIILLDGIASSSGYSPLTPVSMEIFANARTIALNNTKIDVLF
                     HHGSIDAPEYSVFDFSMNSIFEGLSYTMYSSYTEVNSTDQGWHLISGNGILKSY"
BASE COUNT          589 a          445 c          421 g          670 t
ORIGIN      
        1 ataagggtta aaagattttg attggtattc gattctttag ttatggaaaa aaatgtaatt
       61 tcgtggccta acttattgcg atgactacag aatgcggttt gaagggggga ttcaaatgcc
      121 tgatattttg aaaaatagcc attaaaaact taataatcaa tgaaaatcca caaaattttt
      181 tctttattaa ttttcggttt aagttacttt cctttaattt cttttggcca agcccggtta
      241 cagtttattc ataattctcc cgatgtgagt ttagattcga ttgatattta tgtaaataaa
      301 actctgatgc tcgataatgt gaaattcagg gaggcttcgg agtttattca ggggccgtcg
      361 ggaattccgc ttttaatcga aatttgtccg ggaaattcga ttaattccga aagtgcattt
      421 tatagcactg ctgtcatgtt ctctcaaggg aaaacttata tagccattgc gcaaggcatt
      481 atcgatggga atggctatca tccgtggcaa ccgttcgagt tacagatcat cgattatggg
      541 cgtgaaactt cgaatacacc tggccaaacc gatgtattgt ttttcggtgg ctcaaccgac
      601 gctcctgcct tcgacattat agaaacaact tctttaggac ttctgagtga cgatctaact
      661 tatgggcaat tcgatggtta caaggagtta accccagcca actatgtgct cgaaataaag
      721 gattcaacca gtggaacacg atattttcgt ttacagttcg atttgacaac ctattcgggt
      781 caagccttcg ttctaatggc ttcgggattt ctggtccccg gcttaaacaa caatggagaa
      841 aatctgggtt tctatatcgt ttcggcgaat ggaggtatgg gtgtgtcctt gcctcaaata
      901 cccgacccaa tggcatcaat ccagatcgtt catctttgtg ccgatcgagc gctcgatagc
      961 atggatgttt ggattggaaa taaaaaattc ggtgcagatt tacctttcgg aaattctttg
     1021 ccttttatcg agattcttgc agaaaagaat caagtgattg cgattcaacc cgctgggagc
     1081 acttcccccg ataatcctct cgtttccctc tcctatactt ttcacgatcg acaaaagtat
     1141 atacttatgc ttaccgggat tacaagtgaa acaggatata caccggcccc tgccctacag
     1201 atttatcctt tcgatagtgc ccgaattgaa gctaactcgc caaatgctac cgatatgttg
     1261 gtatttcatg gttcgaccga cgctccaact atcgaacttt acgaaacagg aataggtctg
     1321 ggtttattag tggagaattt agaatatggg aattttgcat ctgagggcta tttatctctt
     1381 accactgccg actatgtcat cgaagttaga aataaaccgg gtaatgaagt cttatatact
     1441 tattctttgc cactttcagc actcggttta caaggtgaag ccttgtcgct ttgtgtcgct
     1501 ggttttgtaa atcccacaaa taatagcaat ggtcctgctt ttggtttgta tctctccgga
     1561 aagaatggag gagccttgtt cgaactgcca atttatattc ctcccactac ctcatttgta
     1621 cagttcatac ataatattgc cgatacctcg gctcaaagcc tcgatatctg gatgaataat
     1681 cagatgctcc tcgataacca tctgtttcag aaagcttctc cctatctcga agttccggca
     1741 aaccaaaatc tgacatttag cgttttaccc caaacttcca tttcaccgga taatcctcta
     1801 acaagcctga atctgacact tgaatcggga aaacattata taatccttct tgatggtata
     1861 gctagttcaa gcggatatag tcccttaaca cctgtttcta tggaaatctt cgctaatgct
     1921 cgtacaattg ccttgaacaa tacaaagatc gatgtgctgt ttcatcatgg gagtatcgat
     1981 gccccagaat attcggtatt cgatttttcg atgaacagca tattcgaagg actttcgtat
     2041 accatgtaca gttcttatac agaagtcaat tccactgatc aaggttggca tctcatctcg
     2101 ggaaatggaa ttctaaagag ttacc
//
LOCUS       DAORPD010000251         2120 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_9562_length_2120_cov_1.453269, whole genome shotgun sequence.
ACCESSION   DAORPD010000251 DAORPD010000000
VERSION     DAORPD010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9562_length_2120_cov_1.453269"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="PK976_03795"
     CDS             <1..888
                     /locus_tag="PK976_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67041.1"
                     /translation="VKITGADNVTIYVGDDLYSDYYENRGWNTSITFGWGWPPYYSWY
                     YGSRYRPWHYSSWYYPWYDPWYSYWYDPWYYGGWYSPWYYSWYSPGYYGGWYSPWYGS
                     PWYGGGYYSNIRYNRSGRSSGSYRSSASGVSTDSRIISGNSRSSRSTTVGTSAGRSSY
                     RSSQIAPRTRIVDESGRIYNSRTGETVSRTISPSTRSSSAATVDRSGNTNSTRQSSNT
                     YQRPSNNSSTRQSATTGRSYSTPSRSESTSRSSTYSTPSRSYDNSSTRSSSVGSSSSS
                     RSLSGSSGGSGRSSGGGRR"
     gene            1048..>2120
                     /locus_tag="PK976_03800"
     CDS             1048..>2120
                     /locus_tag="PK976_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67042.1"
                     /translation="MAFSQGEVEALKLSGSDLSGTARGMAMGGAFGALGGDLTGISIN
                     PAGIGVYRSSEVVGTLGLAQEKSEVGSVTRNKTQFAFDNIGFVGYFPLRNDAIPFLNF
                     GFTYNNIKNFDKNIATPFGSPNSSLMDYMSYVSNSFNDGKGVNPSLLDFNITDDPFSS
                     GAPWLSVFGFNSYLIDRNPNRTQQGYEYDNRPSPLQDEAVDNSISLSERGYVNNYDFT
                     VGTMVANKLNIGVAITVADLYYRLTSRYSEEFSNGENAGFDLRNYLKTDGAGVGAKVG
                     IIYRPVNSFRIGVSYHSPVWYYLTDTYSAEMVEDVSAYFQNTDYESGRTNSDVYYVDY
                     RLKTPDKWIFSLAGVFGNNFIASL"
BASE COUNT          582 a          425 c          470 g          643 t
ORIGIN      
        1 gttaaaatta ccggagcaga taatgttacg atctatgttg gtgacgattt gtattccgac
       61 tactacgaaa acagaggatg gaacacaagc attactttcg gctggggatg gcctccatat
      121 tactcatggt actatggtag ccggtatcgt ccttggcatt acagttcttg gtattatcca
      181 tggtacgacc catggtatag ttattggtat gatccttggt actacggagg atggtattcg
      241 ccgtggtatt attcatggta ttcgcctgga tattatggcg gatggtattc cccatggtat
      301 ggtagcccgt ggtatggagg cggatattac tccaatataa gatataatcg ttcaggcaga
      361 tcttcggggt catacagatc gagtgcatcc ggagtttcta ccgactcccg aataatcagc
      421 ggtaatagtc gaagttctcg atctactaca gtcggaacct cggccggacg ttcatcttac
      481 agaagttcac aaatcgctcc tcgtacacgt atcgttgacg agtcgggtag aatttataat
      541 tcgagaacgg gagaaactgt aagccgaacc atttctccat caactcgttc ctcttccgca
      601 gcgacggttg atcggtccgg aaatacaaat tcaacacggc aaagcagcaa tacttatcaa
      661 cgtccgtcga acaattcgag cacaaggcaa tccgccacaa ccggccgttc gtattctact
      721 ccttccagga gcgaatctac aagccgcagt tccacatact cgactccttc gagaagttat
      781 gacaattctt ctactcgttc ttcttctgtc ggctcttcat cgagttccag aagtttgtcc
      841 ggatcttcgg gcggcagtgg acgaagttca ggtggcggtc gcagataaat tcttaaatat
      901 ttgactgtct tggtataaat tccattatga atgaattata gtgagacagt cattttttta
      961 taaatgcaga ttaaaataat catatacaat acaataatga aaaaatcgat aattgttttt
     1021 tgctctgttc tgatgtgttc aatctccttg gcattttcgc aaggtgaagt tgaagcattg
     1081 aaattgtcgg ggagcgattt aagcggaaca gctcgtggta tggcaatggg tggagcattt
     1141 ggtgcattgg gaggagatct gaccggcatt tccatcaatc cggccggtat tggggtctat
     1201 cgaagttccg aagtagtagg tacattgggc cttgcgcaag agaaatcgga agtgggaagc
     1261 gtcacgagaa acaaaactca atttgctttt gacaatattg gttttgtggg ttattttcct
     1321 ctgcgaaacg atgccatccc ttttttgaat ttcggattta cctacaataa tattaaaaac
     1381 ttcgacaaaa atattgccac cccttttggt tcgcccaaca gctcattgat ggattatatg
     1441 agttatgttt ccaattcatt taatgatggc aaaggagtta atccatcttt attggatttc
     1501 aacattaccg atgatccttt ttcatcagga gctccgtggc tttcggtttt cggttttaat
     1561 agctatttga tcgatcgaaa tcccaatcga acgcagcaag gatatgaata tgataatcga
     1621 ccttcacctt tacaagacga agcagttgat aattcaatat cgctttcgga aagaggatat
     1681 gtcaacaatt atgattttac ggttgggaca atggttgcca acaaactgaa tattggcgta
     1741 gcaatcactg tggccgattt gtattatcgt cttacatcac gttattcgga agaattttca
     1801 aatggagaaa atgccggttt cgatttacgc aattacctga aaacagacgg agcaggagta
     1861 ggggcaaaag tcggcattat ttacagacct gtcaattctt tccgtatcgg cgtatcgtat
     1921 cattcccctg tttggtatta tctaaccgat acttattcgg ctgaaatggt ggaagatgtt
     1981 tctgcctact ttcaaaatac cgattatgaa tcaggtagaa caaactctga tgtttactat
     2041 gttgactacc gactgaaaac tcccgataaa tggatattta gtttggccgg agtattcggt
     2101 aataatttta ttgcaagcct
//
LOCUS       DAORPD010000252         2119 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_9570_length_2119_cov_2.258721, whole genome shotgun sequence.
ACCESSION   DAORPD010000252 DAORPD010000000
VERSION     DAORPD010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9570_length_2119_cov_2.258721"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1801
                     /locus_tag="PK976_03805"
     CDS             <1..1801
                     /locus_tag="PK976_03805"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HRC67043.1"
                     /translation="RITHTPFSEYEARWTPDGKITFISSESGTPQIWIMDKNGKNRKQ
                     LTRVENGVNGYKFSADMSLLVYVSDVPRQRIKKKLFEGLDKTSGRLMNDLMYRHWDSW
                     VDAFSHIFITTFNGQPVSQGKDIMNGEPWESPIRPFGGMEQVAISPDSKTIIYTCRKK
                     QGKEYALSTNTDLYAYSLATGQTTNLTSGMMGYDMEPVFSPDGKYLAWWSMERDGYEA
                     DQQRLFLLNLFTGEKIYASQGFDGDVSQITWSKHGDLIYFISNHQATYQIYSYSMADG
                     EIRQVTDGMHDFKTVLDGGDKLYGIRMSIQQPDEIFSIDPSNGEFTEISFINKDLFAR
                     LTLGKVEERWTKTTDGKMMKSWVIFPPHFDSTKSYPTLLFCEGGPQNATSQFWSYRWN
                     FQLMAANGYIIVAPNRRGVPGFGQEWKEQVSGDYGGQNMKDYLSAIDDVTSEPWSDEN
                     HLGAIGASYGAFSVYWLAGHHNKRFKAFIAHDGMFNLEAQYLETEEMWFVNWDLGGPY
                     WDKDNAIAQRSFANSPHRFVDKWDTPILIIHSEKDYRIVASQGMSAFNAAVLRGIPAE
                     FLYFPDENHWVLQPQNSVLWQRVFFDWLDQWLK"
BASE COUNT          616 a          433 c          481 g          589 t
ORIGIN      
        1 ccggattacg catacaccat tttcggagta tgaagcccga tggacccccg acggaaaaat
       61 tacctttatt tcttccgaat cgggcacacc acaaatttgg ataatggata aaaatgggaa
      121 aaaccgcaaa cagcttactc gagttgagaa tggagtaaat ggttataaat tttcagccga
      181 tatgtcgctg ttggtttatg tttcggatgt tccccggcaa cgaatcaaga agaaactttt
      241 cgaagggctc gacaaaacca gcggccgatt gatgaatgac ttgatgtatc gtcattggga
      301 ttcatgggta gatgcttttt cgcacatatt tattacaact tttaacggcc agccagtaag
      361 ccaaggcaaa gacatcatga atggcgaacc atgggaatcg cccataagac cattcggtgg
      421 aatggaacaa gtagccatta gccccgatag caaaaccatt atttacacat gtcggaaaaa
      481 acaggggaaa gaatatgccc tgagcaccaa taccgatttg tatgcttatt cgttggcgac
      541 tggacaaact actaatttaa cttcaggaat gatgggctat gacatggaac ccgtattttc
      601 acccgatggt aaatatctgg catggtggag catggaaaga gacggctacg aagccgatca
      661 acaaagattg tttttgttga atttattcac tggcgaaaaa atctatgcca gtcaaggatt
      721 cgacggggat gtaagtcaga ttacatggag caaacatggc gacctgatct attttatcag
      781 taatcaccag gctacttatc agatttattc gtattctatg gcagatggcg aaatccggca
      841 agttaccgat ggaatgcatg attttaaaac agttttggat ggaggggata aactttatgg
      901 gattagaatg agtatccaac aacccgacga aatcttctcc atcgatccca gcaatggaga
      961 atttactgaa atttcattca tcaacaaaga tttatttgct cggcttactc tgggaaaagt
     1021 tgaggaacgc tggaccaaaa ccaccgatgg aaaaatgatg aagtcgtggg ttattttccc
     1081 tcctcatttt gacagtacaa aatcttaccc caccctgctt ttttgtgaag gaggtcctca
     1141 aaatgctacg agccagtttt ggtcgtaccg atggaatttt cagcttatgg cggctaatgg
     1201 atatataatt gtagctccta accgacgcgg agtaccgggt ttcggacagg aatggaagga
     1261 acaggtctcg ggagattatg gtggccaaaa catgaaggac tatttatcgg ctattgacga
     1321 tgtaacgagc gaaccctgga gcgacgaaaa ccatctgggt gctattggcg caagttatgg
     1381 agcattcagt gtttattggc tggcagggca tcacaataaa agattcaaag catttattgc
     1441 tcatgatggc atgttcaatc tcgaagctca atatctcgaa actgaagaaa tgtggtttgt
     1501 aaattgggac ctcggcggtc cttactggga taaagataat gccattgccc agcgctcgtt
     1561 tgccaattcg ccccatcgct ttgtagataa atgggataca ccgattttaa tcattcacag
     1621 tgaaaaagat tatcgaatcg tggcctcaca gggtatgagc gcctttaatg ccgctgtgct
     1681 tcggggaata ccagccgagt tcctttattt tcctgacgaa aatcattggg tattgcaacc
     1741 tcaaaactct gtgctctggc aacgagtctt cttcgattgg ctggatcaat ggttgaaata
     1801 ggctaccatc catcgaacca taattgcctg atttagattt ttttctgtat ttcgctgagt
     1861 ctatgacctc actggaatat gttggcaggc tttcaaaaga taggatgaga tcgaatatac
     1921 tcccctcttc ttttttatcc tgaattatct ataaaaaaac ttgaaaggcg cttatctcga
     1981 tgcgctatga cggattactc gacagtcaat gaaggtaggt ggccgagtag ggcgaagccc
     2041 gtatcgaggc cactataaat aattcgtgct catcgcaatt tgagctattt ccctttctct
     2101 attatatttt cctgcctct
//
LOCUS       DAORPD010000253         2112 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_9625_length_2112_cov_2.226544, whole genome shotgun sequence.
ACCESSION   DAORPD010000253 DAORPD010000000
VERSION     DAORPD010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9625_length_2112_cov_2.226544"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            16..>2112
                     /locus_tag="PK976_03810"
     CDS             16..>2112
                     /locus_tag="PK976_03810"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HRC67044.1"
                     /translation="MLSWCFDRGNLRGWGANVGKWGEVEVNGLIGEVNENEGYAFVMN
                     GFQQAGALTPLAKYDKRYARALGKWLANLASSSRYFYWNALPSDYQDSYSWASQYDTS
                     ACIPYEALKSTWQGKSPFARGDAIAGGWAATNLSLYSGSSVGYLGAMIKRTSVDQILA
                     FDLNQTDFLVKDSIPAFLLYNPNDSNLTCSFDLPSGNWKVYESLTETFFPEVISGSFT
                     LNIPADEACLIRLYPASETCTIEGNHLVCNGKIIDYQYHYNFSKKLRIKALESEPNPV
                     EKNASFRVWVKADQIPEGVVPVYFWNFNGTQITTLVDSTHLKTPSQTGLYVLTLKVQA
                     GDQWAEDSLRIEVVDWIPKPPLIDSISVSKFYCNPLEKITLEIWADTTGSGPYNFSWT
                     SSAGQISFSNNIAEFVAPSAEQIVSITGKITNSLGLSSTKSIEILVRDTLHHSAQPLI
                     WYPFDTSDSNAVTNKFHATSIGTIKAPDARNIENKAKRFISNSDLIYTENEPELDFSY
                     PLTLSCWIYPEKIYQEHYIISHGSWQERFKLSLLSDHHLRLTVNTTSGIFDLDSKNPI
                     PLEQYTHVTGIYTGFSLEIYINGKLDNFMSCSGNLNPTSHPLTLGRMNEIETKYSFWG
                     RIDEFKLWNDALSLSEVKNLPFIWFSLADGQKLHVYPTLLRKGESIKISGIEAGSEVK
                     LYSVFGQVVGINLQINP"
BASE COUNT          612 a          469 c          429 g          602 t
ORIGIN      
        1 tacaatctca caaaaatgct cagttggtgt ttcgatcgtg gaaatttgcg aggctggggc
       61 gccaatgtcg ggaaatgggg tgaagtcgag gtaaatggcc tcattggtga agtaaatgaa
      121 aacgaaggat atgcttttgt gatgaatgga tttcagcagg caggtgcact tacacccctc
      181 gcaaaatacg ataaacgtta tgctcgcgca ttgggtaaat ggttagccaa tctggccagc
      241 tcctcaagat atttttactg gaatgctctt ccatccgatt atcaagatag ttattcatgg
      301 gcttcacaat atgatacttc ggcttgcatc ccttatgaag ctctaaaatc gacttggcaa
      361 ggtaaatcgc cttttgccag aggtgatgcc atagctggag gctgggctgc taccaatctt
      421 tctctatatt caggatcaag tgttggctat ctgggcgcaa tgatcaaaag aacttcggta
      481 gaccagatcc tcgctttcga tctgaatcag actgattttc tggttaaaga ttctatccct
      541 gcatttttac tctataaccc taacgattcg aaccttacat gcagcttcga tcttccctca
      601 ggaaactgga aggtatacga aagccttacc gaaacctttt tccctgaagt aatctccggt
      661 tcgtttacac tgaatattcc cgccgacgaa gcctgtttga taagattata tccagcatca
      721 gagacttgca caatagaggg aaatcacttg gtttgtaatg gaaaaatcat cgattaccaa
      781 tatcattata atttctcgaa gaaattacgc atcaaggcat tggaatcaga acccaatccc
      841 gttgaaaaaa atgcttcatt tcgcgtctgg gtcaaagccg accagattcc cgaaggtgta
      901 gttcctgtct atttttggaa tttcaatgga acacagatta caaccctcgt cgattccact
      961 catttaaaaa ctccctcgca aaccggtctg tatgttttga ctctcaaagt acaggcagga
     1021 gaccagtggg ctgaagattc ccttcgaatc gaagtggtcg attggattcc taaaccccct
     1081 ctcatcgatt ctatcagcgt ttccaaattt tactgcaatc cactggaaaa gatcacgctg
     1141 gagatatggg ctgataccac agggagcggg ccttacaatt tttcatggac atcaagtgct
     1201 ggtcagatat ctttttcgaa taatattgct gaatttgtgg caccttcggc cgaacagatc
     1261 gtcagcatca ccgggaaaat aaccaattct ttgggattaa gctctacgaa atcgatcgaa
     1321 atattggtta gagatacctt gcatcattcg gctcaaccct taatctggta tcctttcgac
     1381 acgagtgata gcaatgccgt aaccaataag tttcacgcca cttctatagg cacaatcaag
     1441 gcacccgatg cccgaaacat cgaaaataaa gctaaacgtt tcattagcaa cagcgatttg
     1501 atttacaccg aaaatgaacc cgaactcgac ttttcttatc ccttgactct gagctgctgg
     1561 atatatcccg aaaaaatata tcaggaacat tatatcatct ctcatggtag ctggcaggaa
     1621 aggttcaaac tgtcgctttt gtctgatcat cacctcaggc tgacggtcaa tacgactagc
     1681 ggaattttcg acctcgacag caaaaacccc attcccttgg aacaatatac ccatgtaacg
     1741 ggcatttata ctggtttttc gcttgaaatt tacatcaatg ggaagttaga caatttcatg
     1801 tcgtgcagcg gtaatcttaa cccaacctcc catcctctta ccttgggtcg aatgaatgaa
     1861 atagaaacca agtatagttt ttggggtcgt atcgacgagt ttaaactttg gaatgatgca
     1921 ttgtcacttt ccgaggtaaa aaatcttcct ttcatctggt tttctttagc cgatgggcaa
     1981 aaattacatg tttatcccac tttgcttagg aaaggagaaa gtataaaaat ttccggaata
     2041 gaagcaggct ctgaagtcaa attatattcc gtgtttggac aagtcgtcgg cataaatttg
     2101 caaataaacc cg
//
LOCUS       DAORPD010000254         2105 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_9675_length_2105_cov_2.445366, whole genome shotgun sequence.
ACCESSION   DAORPD010000254 DAORPD010000000
VERSION     DAORPD010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9675_length_2105_cov_2.445366"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="PK976_03815"
     CDS             complement(<1..402)
                     /locus_tag="PK976_03815"
                     /inference="COORDINATES: protein motif:HMM:NF014656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HRC67045.1"
                     /translation="MAYYVKNKYSSRRRRTSGQKIIFGVLIVCIVLALIIAYGLYSAL
                     FKPNVWTKGENIVYINIPTGSNFEDLKNMMYERGLIVNRKTFEWLARQRKLDKHVIPG
                     RYPLTNQMSNLELIRMLSGGHQVPVQLVFNNM"
     gene            complement(406..936)
                     /locus_tag="PK976_03820"
     CDS             complement(406..936)
                     /locus_tag="PK976_03820"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HRC67046.1"
                     /translation="MIQGDNIRLRALEMEDADWIFHFENDPSLWRVTQTVTPYSRYAI
                     EQYVLNASIQDIYGSRELRLVIETLRDKRPVGLIDLFEFDPLNRRAGVGVAIEQKERR
                     QGFASEALQLLIQHCFRVLALHQLYCNILVSNEASIQLFEKFGFEVTGRKKEWIIKHN
                     QWEDELFLQLFNPQTI"
     gene            complement(939..1733)
                     /gene="dapF"
                     /locus_tag="PK976_03825"
     CDS             complement(939..1733)
                     /gene="dapF"
                     /locus_tag="PK976_03825"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HRC67047.1"
                     /translation="MTVNFAKYHGTGNDFILIDNRTDFFSVNKEFISNLCHRRFGIGA
                     DGLILLNKANGFDFEMLYFNSDGNQATMCGNGGRCIVAFAQKLGIIQQNAIFKAVDGL
                     HTARILEVDKHDTLIELSLNDVELYHFVGADCLLDTGSPHLVRFMEYSDLRKFNVKKE
                     GRKIRYRSPYTNEGINVNFVCETSEGIFVRTYERGVEAETLSCGTGVTASALAWSLNK
                     NLLRGPVEVFTRGGNLTVDFEKADEKFIHINLTGLATFVFTGKYSI"
BASE COUNT          673 a          444 c          367 g          621 t
ORIGIN      
        1 catattgtta aatacaagtt gtacaggcac ctgatgtcct cccgagagca tgcgtataag
       61 ttcgagattg ctcatttggt tggtcaaagg gtagcgcccg ggaataacat gcttatcgag
      121 cttgcgctgg cgcgctaacc attcgaaagt tttcctgttg acaatcaggc ctctctcata
      181 catcatattc ttcaaatctt caaaattaga gcccgtagga atatttatat aaactatgtt
      241 ttcgcccttt gtccatacat tcggcttaaa cagggcagaa taaagaccat aagctatgat
      301 gagtgctaaa acaatgcaaa caatcaatac tccaaaaatg atcttttgcc ctgaagttcg
      361 cctccgcctc gaagaatact tattttttac ataatatgcc atatcttaaa tggtttgagg
      421 attgaataat tgcaagaaaa gctcatcttc ccattggttg tgtttgatga tccattcttt
      481 tttccggccg gtgacttcga atccaaattt ttcgaaaagt tggatgctgg cttcattcga
      541 gacgaggata ttgcagtata gctgatgaag cgctaaaacc cgaaagcaat gctggatcaa
      601 tagctgaagt gcttcgctag cgaagccctg tcttcgttct ttctgttcga tggcaacacc
      661 cacaccagct ctccggttca agggatcgaa ctcgaaaagg tctatcaaac ctacggggcg
      721 cttatcgcga agagtttcta tcaccaagcg gagttcacga ctaccataaa tatcctgtat
      781 gcttgcattc aaaacatatt gttcgatagc atagcgtgaa taaggtgtaa ccgtttgcgt
      841 aaccctccat aaggaaggat cattttcgaa atgaaaaatc cagtcagcat cttccatttc
      901 caaagcccta agacgaatat tatctccctg tatcatgatt atatcgaata ttttccagta
      961 aatacaaaag tggcaagccc tgtaagatta atgtgaataa atttttcatc tgccttttca
     1021 aaatccactg tcaaattacc tcctctcgta aaaacctcta ccggacctct gagcaaattc
     1081 ttattaagac tccaggctaa agccgaggcc gtaacaccag ttccacaact taaagtttct
     1141 gcttctacgc ctctttcgta agttctaacg aaaatacctt ccgaagtttc acatacaaaa
     1201 tttacattga ttccttcgtt cgtataggga gatcgataac ggattttcct gccctctttt
     1261 ttcacattga attttcgaag atcagaatat tccataaaac gtaccaaatg tggcgaacct
     1321 gtatcgagta aacaatcagc cccaacgaaa tgatataatt ccacatcatt caacgataac
     1381 tctattaggg tatcatgctt atctacttct aatatcctcg cagtatgaag tccatcgact
     1441 gctttaaaaa ttgcattttg ttgaataatc cccagctttt gagcaaaagc cacgatacat
     1501 cgccctccat tgccgcacat ggtagcttgg ttgccatcag aattgaaata aagcatttcg
     1561 aaatcaaatc cattagcctt gttcagtaaa atcaggccgt cagctccaat cccaaaacgg
     1621 cgatggcata agttagaaat gaattcttta ttcactgaaa aaaagtcagt tcggttatct
     1681 atcaatatga aatcattccc agttccatga tatttggcaa aatttacagt catttgttca
     1741 catcaattaa accacattaa acttatatta aaaagcctcg aatttaaaca gaaatgaaaa
     1801 tattgtcaaa attaccgaag cgaaattagt atgaaaatga atcaaggtat aaaaattgaa
     1861 tgaaatttgt agaataaaaa aatagttaaa agtatgaaag ttatatttaa aaatttaaaa
     1921 atcaatgaca tgaaaaaata tctttccttg ttgcttgtag ctattatagg tgggatgatg
     1981 gcggtaggta tttataaagc tgttgaaccc aagcaatcgg atctccaaaa tagagaaata
     2041 attccggtgc agcaagttag ccttacggat gcgcatgcca tgcctctgcc cgattttgtc
     2101 gaagc
//
LOCUS       DAORPD010000255         2103 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_9686_length_2103_cov_2.882812, whole genome shotgun sequence.
ACCESSION   DAORPD010000255 DAORPD010000000
VERSION     DAORPD010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9686_length_2103_cov_2.882812"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..914)
                     /locus_tag="PK976_03830"
     CDS             complement(<1..914)
                     /locus_tag="PK976_03830"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67048.1"
                     /translation="MRNPLRLFTDHLYYVSKGLLFLCALIIILLFFPREGKFKYEYVK
                     GKPWLHEDLIAPFDFSVLKTDEEYELQKERLLAKSRLYFTYQPEVTANQRVIVAQALI
                     DFLMMSPSDTLSGKPIAEVYRKAIQIADTIFRHGIIQMVPELIALPSGQSIMIVKGNI
                     ATEAYPEHFFTVQSADAYIVKHLSGIDSAYRRKVRQALEMGLAHNIRFDKELTEKSQR
                     QLLANLSRVKGLIQQGEKVISKGEVVDEEKFQVLESLRIETEKRLGTGHARFAVFIGQ
                     LILVSLAMLVLGLFLYFFRPHIFADNHQV"
     gene            complement(1056..>2103)
                     /locus_tag="PK976_03835"
     CDS             complement(1056..>2103)
                     /locus_tag="PK976_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HRC67049.1"
                     /translation="FEGLGKERIKTPVEAGDIVAVLGPEEFEIGNTIADFENPEAIPP
                     IKVDEPTMSMIFAINNSPFYGKEGKYVTSRHLRDRLFLETQKNLALRVEETFQPDTYM
                     VYGRGVLHLSILIETMRREGYEFQVGQPKVIIREMNGEKCEPIEFLSINVPEEFTGKI
                     IDIVTSRKGEIEGVCTRNNRAYLEFSIPSRGIIGLRNAVLTATEGEAVMSHRFKGFET
                     WKGDIPHRRNGVLIAMETGEAITYSLDKLQDRGTFFIEPGQNVYAGQIIGENSRSTDL
                     VVNVIHTKKLTNIRAAGSDEKAILSPPRIFSLEEALEYIAADEYVEVTPNAIRMRKII
                     LDHLKRKRENKTEE"
BASE COUNT          602 a          459 c          417 g          625 t
ORIGIN      
        1 acctgatggt tatcggcaaa aatatgaggc ctgaaaaagt acaagaacaa gcccaatacg
       61 agcatggcaa gactaaccag aatcaactgc ccgatgaaaa ccgcaaatcg agcatgcccc
      121 gtgcccaatc gtttctcggt ttcaatacgt aaggattcaa gaacctgaaa tttctcttca
      181 tcgaccactt cacccttcga aattactttt tcgccttgtt gtatcaatcc ttttactctg
      241 gacaaattag ccagcaattg tcgttgcgat ttttccgtga gttccttatc gaaccgaata
      301 ttatgagcca atcccatctc gagagcctgt cggacttttc ttctataagc agagtcaatt
      361 cccgataaat gtttcactat gtaggcatcg gcagattgta ccgtaaagaa atgttcagga
      421 taagcttcgg tggctatatt gcctttgaca atcatgatgg attggccgga aggcagggca
      481 ataagttcgg gaaccatctg aattattcca tgtcgaaaga tggtatcggc tatttgaata
      541 gcttttcgat aaacttcggc aataggtttg ccggaaagag tatcagaagg cgacatcatt
      601 aaaaaatcga tcagggcctg agcaactatt actctctgat tggcagtaac ttcaggttga
      661 taggtaaaat acaaacggct ctttgccaat agcctctctt tctgcaactc atattcttca
      721 tccgttttca ataccgaaaa atcgaatgga gcaatcaaat cttcgtgaag ccagggtttt
      781 cccttcacat attcatattt gaactttcct tcacgcggga aaaataacag aataattatc
      841 aaagcacata aaaaaagcaa gcccttgctt acataataca gatgatcggt gaacaagcgc
      901 aaagggtttc tcatgcaatt tcaaattgaa tttcaaattc tggtggataa acttgcatag
      961 ggacaagaga atttgagaaa tagccaaatc gataatcctg aaaaatagaa gacatttttc
     1021 aaggatggat ttgccacgaa aataaacaaa atctcctatt cttcggtttt attttcgcgt
     1081 tttcgtttca aatgatcgag aataatcttg cgcattctga tggcatttgg ggttacttcg
     1141 acgtattcat cagctgcaat gtattcgagg gcttcttcga gagaaaagat gcgtggaggt
     1201 gaaagaatgg ccttttcatc acttccggct gctctaatat tggtgagttt cttcgtatgt
     1261 atgacattga ccacaaggtc ggtactgcga ctattttcgc cgataatttg acccgcatag
     1321 acattttgcc ctggttcgat gaaaaaagtt ccacgatctt gaagtttatc gagggaatat
     1381 gtgatagcct cgcctgtttc catagctata agaactccgt ttcgtcgatg agggatatct
     1441 cctttccatg tttcgaatcc cttgaaacga tgggacatca ccgcttctcc ctcggtagca
     1501 gtcagcactg cattgcgcaa tcctataatt cctcttgaag gtatgctaaa ttcaagataa
     1561 gcccgattat tacgtgtaca aaccccttcg atttctccct tgcgtgaagt tacgatatcg
     1621 ataattttac ccgtaaactc ttcgggtaca ttaatagaaa gaaattcaat gggttcacat
     1681 ttttctccat tcatttcacg gataattacc ttaggctggc ctacctgaaa ttcgtaccct
     1741 tcccttcgca tagtttcgat gagtatagaa agatgaagca ctcctcgacc gtaaaccata
     1801 taagtgtcgg gctgaaatgt ttcttctacc cttaaggcaa gattcttttg cgtttcgagg
     1861 aacaatctat cacgaagatg gcgagaggta acatattttc cttccttccc gtaaaacggg
     1921 ctgttattga tggcaaagat catgctcatg gtcggttcat ccaccttgat gggagggatg
     1981 gcttctgggt tttcgaaatc agcaatcgta ttgccaattt cgaattcttc gggtcctaaa
     2041 acagccacaa tatctccggc ttcgacggga gtttttatcc tttccttacc taaaccctcg
     2101 aaa
//
LOCUS       DAORPD010000256         2095 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_9728_length_2095_cov_2.244118, whole genome shotgun sequence.
ACCESSION   DAORPD010000256 DAORPD010000000
VERSION     DAORPD010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9728_length_2095_cov_2.244118"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2079)
                     /locus_tag="PK976_03840"
     CDS             complement(<1..2079)
                     /locus_tag="PK976_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67050.1"
                     /translation="MDKVLNESMLHRYKRIKATRLDKDGQVIYYTYRHRVLLASFSPE
                     ILAMSLQQLNKPHWISNNPQIENLLKTAGNHVDGTLIFNFDYLPEIFKKWPASDFIPG
                     FGKLKKFAHYASLDLYFQKKKIILNGYSLTRPGDFLRLFRNQAPQEPTALNLLPEHTA
                     SYIYLCFDNFDNFIDSYNDYFASFSSPQQPSQQLSFSAIQNLKESQIAEIASGLINTG
                     SETALDNSLVILHSLNPQHFERMIKETLPSTETGLSFTINQREFRKIDFNRFFGNFLS
                     SILPDFQKAFFFRIGDYFLFSSKPQILHDYIVQYLCGKTLVNTQGFKALSEISEERTN
                     LWFYYNPPEAVAFHHFLFAAEAADNFDANLPVLTLFDAVSVQVSGEGQFPYISTIIKH
                     RIANEEILALYSSTILSSDTLADSHSRDIRADERLLWKNQLDHPAAQPVVIVNDLVKG
                     KSFLLAFDQRQNTYLFDAAGSLKWKKQIEGSGEAFCRVECIKINNQPVLFFITRSRLY
                     AIDASGRMLAGYPIKLNIPLAGPASIFPQKNSSELRIYYPGTDSLLYCINIKGKPING
                     WNPPKLTGQVVQAVKRFEFEGKTYFAVPYSSGQVTMIQGNGKKAELFEQAFTNSSNSE
                     FYLNETNRKGALLTTDQSGNLVYLSPKGPIEKTVFDNFSKNHFFIYEDFDNNGSRDFI
                     YLDGKQLVIYD"
BASE COUNT          610 a          373 c          465 g          647 t
ORIGIN      
        1 gtcataaata accagttgtt tcccatcgag atagataaaa tcgcgactgc cattattatc
       61 gaaatcctca taaataaaaa aatgattttt cgaaaaattg tcgaatactg ttttttcgat
      121 agggcctttt gggctcaaat agaccagatt tcccgactga tcggtagtga gcaaggcgcc
      181 tttccgattg gtctcgttca aataaaattc agagttggaa gagttggtaa aagcctgttc
      241 gaaaagttcc gcctttttgc cattgccttg aatcatcgtg acttgccccg atgagtaagg
      301 aacagcaaaa taggtctttc cttcgaactc gaaacgtttc acagcctgaa caacttgtcc
      361 ggttagtttt gggggattcc atccgttgat aggtttccct tttatgttga tgcagtacaa
      421 taaactgtcg gttccgggat aataaatacg aagctctgat gaatttttct ggggaaaaat
      481 agatgccggg cctgccagag gaatgtttaa tttgatagga tatcctgcaa gcatcctacc
      541 ggaagcgtcg atggcataaa gccggctgcg ggtaataaag aacagtaccg gttgattatt
      601 gattttgata cattctactc tacaaaatgc ttctcccgat ccttcgattt gttttttcca
      661 tttcaaagag cccgctgcat cgaataaata ggtattttgt ctttggtcga atgccagaag
      721 gaagcttttg cctttgacca aatcattgac aatgactact ggctgtgcgg ctggatggtc
      781 aagctggttt ttccataata agcgttcgtc tgctcgaata tctctcgagt gcgaatctgc
      841 gagagtatca ctcgatagga tggtggaaga ataaagtgcc agaatttctt cgtttgctat
      901 gcgatgtttg ataatggttg agatgtaggg gaattgtcct tcacccgaga cttgaaccga
      961 aactgcatcg aaaagagtga gtacgggtaa gttagcgtcg aaattgtctg ctgcttctgc
     1021 tgcaaataag aaatgatgaa aagccacagc ttcaggggga ttataataga accacagatt
     1081 ggttctttct tccgatattt ctgagagagc cttaaatcct tgcgtgttca ccagagtttt
     1141 cccgcacaga tattgaacga tgtaatcatg aagaatttga ggtttagaac taaaaaggaa
     1201 ataatccccg attctaaaaa aaaatgcttt ttgaaaatca ggcaaaatac tcgataaaaa
     1261 attgccaaag aagcgattga agtctatttt tcggaattct ctctggttaa tagtaaatga
     1321 tagtccagtt tcggtcgatg gcaaggtttc ttttatcatt ctttcaaaat gctgaggatt
     1381 gagtgaatga agaatgacta gtgaattatc cagagccgtt tcgcttcccg tattgatcag
     1441 tccagaagca atttcggcta tttggctttc ttttagattt tgtatggctg aaaagcttag
     1501 ttgttgagaa ggttgttggg ggcttgagaa agaagcaaag taatcattat atgaatctat
     1561 aaaattgtcg aaattgtcga aacacaggta aatatagctt gctgtatgtt cgggaagaag
     1621 attaagtgct gtaggttctt gaggtgcctg gtttctgaac agtcgtaaaa aatctccggg
     1681 gcgtgtaagt gaataaccat tcagaatgat cttttttttc tggaaataaa gatcgagtga
     1741 agcataatgg gcaaatttct tgagtttgcc aaatcctgga ataaaatcgc ttgccggcca
     1801 tttcttgaaa atctcgggca ggtaatcgaa attgaagatg agtgtaccgt ccacatggtt
     1861 tcccgcagtt ttcaaaaggt tctctatctg agggttgttg gaaatccaat ggggcttatt
     1921 gagttgttgc aagctcatag ctagaatttc gggcgaaaaa gaagctaata aaaccctatg
     1981 cctgtaagta taataaatca cctgtccatc tttatccaat ctagtggctt tgattctttt
     2041 atatctatga agcatagatt cgtttaaaac cttatccata gcattgtttc ccaga
//
LOCUS       DAORPD010000257         2094 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_9732_length_2094_cov_4.794017, whole genome shotgun sequence.
ACCESSION   DAORPD010000257 DAORPD010000000
VERSION     DAORPD010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9732_length_2094_cov_4.794017"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /locus_tag="PK976_03845"
     CDS             <1..721
                     /locus_tag="PK976_03845"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC67051.1"
                     /translation="LIGWMQDVNGNGSLDLIGLTSGSYPLYYLSPTSMPQIIYDNDND
                     IIYAVFVSITEGYHTDSQDYRHLWVRVSYDKGLTWSDFTDLVANIFHIYDECVFPVIS
                     PTLDGGCPAIIYQRDEEPGLSARGDEDPPTTNYITYMKINEYVGVKEVAKHTFTVEQN
                     YPNPSEGMTKVIVEVGQPNRLILSVSTVTGQKVMVIDKGNVNAGQHIFEINTSNLQSG
                     IYFYTVRCGNESMTRKMVVNN"
     gene            901..>2094
                     /locus_tag="PK976_03850"
     CDS             901..>2094
                     /locus_tag="PK976_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67052.1"
                     /translation="MRKFTLIVVCVLVVSGLMAQTTRVKVNGGNIAVKMEKATSEGIS
                     PVNVVSPYVNPVNDAIEEDIGTTIYDLPSNSCTPYGRLYVYPDGTKAGVWTIGKNPTA
                     YPDRGTGYNYYNGSSWGPDPTLRIESVRAGWPSYAPCGPNGEIVVSHQSGTLPLVVSK
                     RETKGSGNWEQSFLNPPSGATGMLWPRMITTGQDHNTVHIIALTAPTGNNGTVFQNMD
                     GALVYCKSEDGGTTFGEWQLLDGLTSEFYLGFGGDSYSWLIPYENDIAFVICDNITDL
                     IIMHSTDNGETWEKIIVWEHPYPFYSSSMATDKFYCPDGSAHGVIDKYGKIHLAFGVN
                     RSYSDGSSSYWFPWVDGLAYWNSDIPVWLGGDTIALHPDTLYEHGRLIGWMQDVNGNG
                     SLDLIG"
BASE COUNT          579 a          367 c          524 g          624 t
ORIGIN      
        1 attaatagga tggatgcaag atgtgaatgg gaatggcagt ttagatttga ttggcctcac
       61 cagcgggagt taccccctgt attatttaag tccgacttcg atgccgcaga tcatatacga
      121 caatgataat gatattattt atgccgtatt tgtgagcata acggagggat atcataccga
      181 ttcgcaggat tatcgtcatt tgtgggtgag ggtttcgtat gataagggat tgacatggag
      241 tgatttcacg gatttggttg ccaatatttt tcacatctat gatgagtgtg tatttcctgt
      301 aatcagtccg acgctggatg gaggctgtcc agcaattata tatcagaggg atgaagagcc
      361 tggtttatcg gcaagggggg atgaggatcc acctacgacg aattacatta cctacatgaa
      421 aatcaatgag tatgtaggag taaaagaggt tgctaagcac acttttaccg tagaacagaa
      481 ttatcctaat ccatcggaag gtatgacgaa ggtgatagta gaggttggcc agccgaatag
      541 attaattttg agtgtaagca ctgttactgg tcagaaggta atggtgattg acaagggcaa
      601 tgtaaatgct ggtcagcata tattcgagat caacacgagc aatttacagt cgggtattta
      661 tttttacact gtacgttgcg gcaatgagag catgaccagg aagatggttg tgaataatta
      721 ggataaaatc gatcttggga aaaggccgtc ctcttcgaag ggatggcctt tttttattaa
      781 ttcatctcat catattttga acttcattca agaatttttt tgaaatcgaa tgatattttt
      841 atgatttgat taaaaattgt attattttta cagcattatt ttaaccaaat aaccaacgcc
      901 atgagaaaat ttactttaat tgtagtttgt gtgctggtag tgagtgggtt aatggcccag
      961 actacccgtg taaaggtaaa cggcggcaac atagccgtga aaatggaaaa agccacttcg
     1021 gaagggataa gccctgtaaa tgtggtgagt ccttatgtaa atccggttaa cgatgcaata
     1081 gaagaggata taggtacgac catatatgac cttccctcca attcttgcac accctatggc
     1141 cgtttgtatg tatatccgga tggtacgaag gccggtgtat ggacgattgg gaaaaaccca
     1201 acagcttatc ccgaccgtgg cactggatat aattattaca atggttcttc atggggaccc
     1261 gatcctactc tacgcatcga aagtgtgaga gctgggtggc catcgtatgc tccttgtggg
     1321 cccaatggcg agatcgtggt ttctcatcag agtggaacct tgcccttggt ggtttcgaaa
     1381 cgtgaaacta aaggcagtgg caattgggaa cagtcatttt taaatccccc ctcgggagca
     1441 acaggtatgt tatggcccag aatgattact acgggtcagg atcataacac agtccacata
     1501 atcgccctta ctgcccctac tggcaataat ggtactgtct ttcaaaatat ggatggcgca
     1561 cttgtttatt gcaagtcgga ggatggaggt actacctttg gagaatggca gcttctcgat
     1621 ggcttgactt cggaatttta tttgggattt ggaggagatt catactcatg gctcattcct
     1681 tatgagaatg atattgcttt tgtaatctgt gataatataa ctgatctaat catcatgcat
     1741 tccaccgaca acggtgaaac gtgggagaag ataatcgtat gggagcatcc ctacccattt
     1801 tattcctcct caatggctac ggataagttt tattgtccgg atggctctgc ccatggagta
     1861 atcgacaagt atgggaagat acatttggct tttggagtga atcgttccta ttctgatggg
     1921 tcgagttcct attggtttcc ctgggtggat ggtttggctt attggaattc ggatataccg
     1981 gtatggttgg gaggcgatac cattgcttta catcccgaca cattgtatga gcatgggaga
     2041 ttaataggat ggatgcaaga tgtgaatggg aatggcagtt tagatttgat tggc
//
LOCUS       DAORPD010000258         2092 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_9753_length_2092_cov_2.545410, whole genome shotgun sequence.
ACCESSION   DAORPD010000258 DAORPD010000000
VERSION     DAORPD010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9753_length_2092_cov_2.545410"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..1394
                     /locus_tag="PK976_03855"
     CDS             156..1394
                     /locus_tag="PK976_03855"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRC67053.1"
                     /translation="MLIIIKLIRESYLMAAAEISVNKTRTFLSLLGITIGIFCVVSVF
                     TVFDSMESTIKSSIESLGNNVVYIQKWPWLFGDDYPWWKYVNRPETKLEELKKLQERS
                     QTAEAMAFLTGTNRTVKSLKHSLKNVTVLAVSYDFFKLRNMEIGEGRYFSPLESNNGR
                     NVTILGNAIAQKLFDGEPAVGRQIKIYGLNTEVVGVLAKEGNNMGESFDEEVIVPVLF
                     AANFLNIDRDGSIAVIAKEGIAKQELIDELTGIMRSLRKLSPGMEDNFSINEISTIST
                     RFDQFFRIMAVIGWVIGGFSLLVGGFGIANIMFVSVKERTNMIGIQKALGAKNYFILL
                     QFLFEAVFLSLLGGILGLFIIFIFTLISTYAFDFRLILTFANIILGVTVSIIIGLIAG
                     IIPSYSAARMDPVEAMRSTF"
     gene            complement(1412..>2092)
                     /locus_tag="PK976_03860"
     CDS             complement(1412..>2092)
                     /locus_tag="PK976_03860"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HRC67054.1"
                     /translation="PGEKLSPTVRKFTDGSGYYDAYNTALVIDTGRELGYYHKSKLVP
                     GPEKLPFPRLTRPIQEVVLNFGGTTGSLGISSERTVFTHRRTGARFNAAICYESVYGE
                     FMTGFIRNGSQLIFVMTNDGWWGNTEGHRQHLTFSSLRAIETRRCIGRAANTGISCFI
                     NQKGDILQSIPYWTKGAIKDQLHLNDNLTFYVKYGDYIGRISALVTILFLFLVFCYNL
                     MKRGKSKF"
BASE COUNT          614 a          382 c          461 g          635 t
ORIGIN      
        1 ccatttataa aaaatgagaa tgcggctgca attttaacca aaatgcattc ataaggcaaa
       61 caattccatt cgaaatgcaa attaatttgt ccttttcaaa ttgagataaa ctcttttcat
      121 gtaggtttgc ataatcataa caatagaatt tttatatgtt gatcatcatt aagctaatca
      181 gggaaagcta cctgatggca gcagctgaaa taagtgtaaa taagacgcgc acctttttat
      241 cgttgctggg gattaccata gggatctttt gtgtggtttc ggtttttact gtattcgatt
      301 cgatggaaag cacaattaaa tcgagcatag aatctttggg aaataatgtg gtttacattc
      361 agaaatggcc ctggttattt ggagatgatt atccctggtg gaaatacgtg aacaggcccg
      421 aaaccaaatt ggaagaattg aagaagctgc aggagcggtc gcaaactgcc gaagcaatgg
      481 cctttttaac tggtacaaac aggacggtaa aaagtttgaa acatagtttg aaaaatgtga
      541 cggttttggc agtttcgtat gattttttca agctgcgcaa tatggaaatt ggcgaaggcc
      601 gttatttttc gcctcttgag tctaataatg gaagaaatgt gacgatttta ggaaatgcca
      661 tagcacaaaa gttgtttgat ggagaaccgg cagttgggcg acaaataaag atttacggat
      721 taaataccga agtcgtagga gtccttgcaa aggaagggaa taatatggga gaaagcttcg
      781 atgaggaggt gatcgtgccc gttctttttg ctgcaaattt tctcaatatc gatcgcgatg
      841 gttccattgc agtcattgct aaagaaggaa ttgcaaaaca ggagctcatc gatgagctta
      901 ctggtattat gcgctcgcta cgcaaattat cgccaggaat ggaagataat ttttccatta
      961 acgaaatcag taccattagt acccgattcg accaattttt ccgcataatg gcagtcatcg
     1021 gatgggttat tgggggtttt tcattgctag tgggtggatt tggaattgcc aatattatgt
     1081 ttgtatcggt aaaagaacga accaatatga ttggcattca aaaagctctg ggtgccaaga
     1141 attatttcat tctcctgcag tttttatttg aagctgtttt tttaagcttg ctgggcggta
     1201 ttttaggcct attcatcata ttcatcttta ccttgatcag tacttatgcc ttcgattttc
     1261 ggctgattct gacatttgcc aatattattc tgggagttac ggtttcgatt attataggat
     1321 tgatagccgg aataattcct tcttattctg cggctcgcat ggatcctgta gaagctatgc
     1381 gatcgacatt ttgagatttg cttaaagaaa ctcagaattt tgatttgccc ctcttcatca
     1441 aattataaca aaaaacaagg aataaaaaca aaatcgtaac caaagcagag atgcgtccaa
     1501 tgtaatctcc gtattttacg taaaaggtaa gattgtcgtt caaatggagt tgatccttaa
     1561 tggcgccttt ggtccaatat ggaatggatt gcagaatatc ccctttttga ttaatgaaac
     1621 acgagattcc tgtattggcg gctcttccta tgcagcgtct ggtctcgatg gcacgaagag
     1681 atgaaaaggt aagatgctgg cggtgtcctt cggtattgcc ccaccaacca tcatttgtca
     1741 tcacaaatat gagctgagaa ccatttcgga taaatcctgt cataaattct ccgtaaaccg
     1801 attcgtaaca tatagcggca ttgaatcgtg ctccggtacg ccgatgtgta aatactgtac
     1861 gctcggaaga aatacccaga cttccagtag tccctccaaa attcaacacg acttcctgga
     1921 tgggccgagt aagccgtgga aatggaagtt tctcggggcc agggactaat ttcgatttgt
     1981 gataatatcc gagctctcta ccggtatcga tcacaagcgc agtattgtaa gcatcataat
     2041 atccgcttcc gtcagtaaac tttcttactg taggagaaag cttttctccg gg
//
LOCUS       DAORPD010000259         2090 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_9766_length_2090_cov_1.819656, whole genome shotgun sequence.
ACCESSION   DAORPD010000259 DAORPD010000000
VERSION     DAORPD010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9766_length_2090_cov_1.819656"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..829
                     /locus_tag="PK976_03865"
     CDS             <1..829
                     /locus_tag="PK976_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC67055.1"
                     /translation="LYTGQALGGFGAAIAANYSWRTVFHGFGIIGIIYAVILILFLND
                     QKSHTSRISETQPLNTKAEKEPFWKPFIVLLSTASFWVMLFYFIVPSLPGWATKNWLP
                     TLFTKNLGIPMTMAGPMATISIAVASLIGVFIGGPLSDRWVLKNIKGRIYTSAIGLTL
                     TVPSLILLGFGHTYISLICSAFLFGIGYGMFDANNMPILCQIISPKYRATAYGIMNMM
                     GVFGGYAITIVLGTSTDKGNLGSDFSKLAFLVLLAVIMVLLFLKPKKEYTYTEENNI"
     gene            863..1786
                     /locus_tag="PK976_03870"
     CDS             863..1786
                     /locus_tag="PK976_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009126756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRC67056.1"
                     /translation="MNFEKIKGLIVAPFTPFDKRGEVDLSPIGDYAMMLQKNGIIGVF
                     INGSSGEGYMMTVEERMKLAEKWISVVDKEFKVIVHVGSTCIKDSCKMAQHAAEIGAF
                     GIGAMASPFPKAGRVEELVKYCEEIACGAPSLPFYFYHIPALSGVCLPMLPFLKAAED
                     RIPNLAGIKYTYESLYEYNQCMLYKNGKFDMLHGQDETILSALVMGGAQGGISGTGNY
                     IGKVLVGVIDEFNKGNIEKARELQNFAQKVINVIVNYRGNIVGGKRIMKLIGIDLGIN
                     RTPFQNMNGEEELIMKRNLEEINFFEKCNKL"
     gene            complement(1866..>2090)
                     /locus_tag="PK976_03875"
     CDS             complement(1866..>2090)
                     /locus_tag="PK976_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007213485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HRC67057.1"
                     /translation="GTQPGKVLRLRNKGLPSVNGFGTGDELIHVNVYVPENLTDKEKK
                     LLSEMEKSPNFEPSKSAKKSVFNKFKKMFD"
BASE COUNT          648 a          412 c          424 g          606 t
ORIGIN      
        1 gctttatacg gggcaagcac tgggtggttt tggggctgca atagcggcca attattcctg
       61 gcgaacagta tttcatggtt ttggtatcat tgggattata tacgccgtta tccttatttt
      121 gttccttaat gaccaaaaaa gtcatactag tcgaatatcc gagacacaac ctttaaacac
      181 aaaagccgaa aaagaacctt tctggaaacc atttattgtg cttttaagta cagcatcttt
      241 ctgggttatg ctattctact ttatcgtccc gagccttccc ggatgggcaa ctaaaaattg
      301 gctaccgaca ttatttacaa aaaacttggg cattcccatg acaatggcag gaccaatggc
      361 cacgatttcc attgccgttg catctctcat cggcgttttt atcggcggcc ctctatccga
      421 ccgatgggtg ctcaaaaaca tcaaaggccg tatctacacg agtgccatag gattaaccct
      481 gaccgtacct tcactcatac tcctgggctt tgggcacacc tatataagcc tcatttgttc
      541 ggcctttttg ttcggtatcg gttatggaat gttcgatgcc aacaatatgc ctatactttg
      601 ccaaatcatc tctccaaaat atcgggcaac agcctacggc ataatgaaca tgatgggagt
      661 ttttggagga tatgctatta caattgtact tggaacttca accgacaaag gaaatttagg
      721 ttccgatttt agcaaattgg cttttttggt tttactggca gtcattatgg tattgctctt
      781 cttaaaaccc aaaaaagaat atacctatac ggaagaaaat aatatttaac taaaataatc
      841 aacatctaaa aaaaattaca tgatgaattt cgaaaaaata aaaggattga tcgttgctcc
      901 gtttacgcca tttgacaaaa gaggcgaagt agatttaagc ccgatcgggg attatgcaat
      961 gatgttgcaa aaaaacggaa taataggggt ttttatcaac ggttcgtcag gcgaaggtta
     1021 catgatgacc gttgaagagc gtatgaaact ggcagaaaaa tggatatcgg ttgtagataa
     1081 agaattcaaa gttatcgtcc atgtcggatc aacatgcata aaagacagtt gtaagatggc
     1141 acaacatgct gctgaaatcg gagcattcgg tatcggggca atggcttcac ctttcccaaa
     1201 ggcaggacgc gttgaagaac ttgtaaaata ttgcgaagaa attgcttgtg gtgcaccctc
     1261 tctgcccttc tatttttatc atattccggc cctaagcggt gtatgcctgc ccatgctacc
     1321 cttcttgaaa gcagccgaag acaggattcc caatctggca ggaattaagt acacttacga
     1381 aagcttatat gaatataacc aatgtatgct atataaaaat ggcaaattcg acatgttgca
     1441 cggacaagat gaaaccattc tttctgcgtt agtcatgggt ggagcacaag ggggaattag
     1501 cggaacaggc aactacatcg gtaaagtact tgtaggcgtg attgacgaat tcaataaagg
     1561 caatatcgaa aaagcgaggg aattacaaaa tttcgcccaa aaagtcatta atgtgattgt
     1621 aaattatcga ggaaacatcg tgggaggaaa acgcattatg aaattaatcg gtattgactt
     1681 gggcattaac cgcacgccat ttcaaaacat gaatggcgaa gaagaattaa taatgaaacg
     1741 caatcttgaa gaaattaatt tttttgaaaa gtgtaataaa ttataatcct tatatttatg
     1801 attaagaaaa gcgaaagtcc acctcattaa actttcgctt ttttcgaaat ctaataatct
     1861 gtttttcagt caaacatctt cttaaattta ttgaaaacgc tttttttagc ggatttgcta
     1921 ggctcaaaat ttggcgattt ttccatctcc gaaaggagct ttttttcctt gtccgtcaag
     1981 ttttcgggaa catatacgtt aacgtgtatc aactcatcac cggttccaaa accattgaca
     2041 gaaggcaatc ctttattgcg cagtcgcagc accttgcccg gttgcgtacc
//
LOCUS       DAORPD010000260         2087 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_9785_length_2087_cov_2.357283, whole genome shotgun sequence.
ACCESSION   DAORPD010000260 DAORPD010000000
VERSION     DAORPD010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9785_length_2087_cov_2.357283"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /gene="aroE"
                     /locus_tag="PK976_03880"
     CDS             complement(<1..624)
                     /gene="aroE"
                     /locus_tag="PK976_03880"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF020090.2"
                     /note="AroE; catalyzes the conversion of shikimate to
                     3-dehydroshikimate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HRC67058.1"
                     /translation="MDNGFSHIYGLIGYPLEHSFSPAYFRQIFEKSGMNNCDYRLFPV
                     RDIESFSFILNDIPHLYGLNVTIPYKEAIIPWLNELSPEAKLIGAVNVIKIIHKGTKI
                     QTIGHNTDAAAFIRTLLPLLRSHHSQAMIFGCGGATKAVAYSLSLLGIPYIIVSRGKK
                     GPHIISYEELSISLINKYPVLINCTPVGMFSYPDEALSIPFEGIGPRH"
     gene            complement(691..>2087)
                     /locus_tag="PK976_03885"
     CDS             complement(691..>2087)
                     /locus_tag="PK976_03885"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRC67059.1"
                     /translation="KMMEEQVEYFFDAEDFEDIIDYYLEQHDSPRAQAAIEAAQHQYP
                     YNSIFELKKAQLLASTNQEEKALKILSRLERIFPNNFEIHYTRSFIYSKLSQHKLAIK
                     ELKKALKLTDNPYEILVTLAFEHEKIWEYEEAIRQLEKALEYDSTEETIINELASFYD
                     MAENLDKGVSYFQKFVEKNPYNVPGWFNLGILYNKLSLYEKSIEAFDFAIAIDPEYSP
                     AYFNKGNAYASASMYEKAIETYRESLQHEAPDANAYCCIGECYQKLQNYDDALFYFKL
                     ATKLDTETYYAWYGIACIHYLQGNLKPALVYVQKALHYFEEFERIKYLEGDIFMALGK
                     YDKAIDTYNRVAANIPKNHDIWFDLASAFDAINDSASAISVLKEGCSYHHNENIDLLY
                     YCIEYIIEHDSVAHALPYIKIAYPLQPSIIEILFMKFPMLKNNKEYLSFLNSLFDKGD
                     NSISEKDDSSPSIN"
BASE COUNT          622 a          400 c          409 g          656 t
ORIGIN      
        1 atgacgagga cctatccctt cgaaaggaat ggagagagct tcatcggggt aagaaaacat
       61 ccccacaggt gtacaattaa tcaaaacagg atatttatta atcaatgaaa tgctgagctc
      121 ttcatacgaa atgatatgcg gacccttttt ccccctcgat acgatgatat atggaatacc
      181 tagcaaactc aagctatatg caacagcttt tgtggcacct ccacatccaa atatcattgc
      241 ctgactatga tgacttctta acagtggcag aagtgttcga ataaatgccg ccgcatctgt
      301 attatgccct atcgtctgta ttttagttcc tttatgaatt atcttgatga catttaccgc
      361 tccaatcagt ttggcttcgg gcgataattc attcaaccat ggtataatag cctctttata
      421 gggtatggta acatttagtc catataaatg aggaatatca ttaagaatga atgaaaagga
      481 ttctatgtcg cgaactggga aaaggcggta atcgcaatta ttcatccctg atttttcgaa
      541 tatttggcga aaatatgccg gagagaagga gtgttcgagg ggatatccta tcaatccata
      601 aatatggcta aatccattat ccattgtatt atgaaaagac ttccaataga ttttcgttcg
      661 aacgaatcga gccgcatgga agtaattatc tcaatttatt gaaggtgaag aatcgtcttt
      721 ttcagagatt gaattatcgc ctttatcaaa cagggaattg aggaaagaca agtattcttt
      781 attgttcttt agcatgggaa atttcatgaa aaggatttcg atgatggatg gttgtaacgg
      841 gtaggctatt tttatataag ggagggcatg agccacagaa tcatgctcga tgatgtattc
      901 aatacaataa tagagcaggt cgatattttc attgtgatga taagagcaac cttccttcaa
      961 aacagaaatg gccgaagctg aatcattgat ggcatcgaag gccgaagcaa ggtcaaacca
     1021 gatatcatga ttttttggta tattcgctgc cacccgattg tatgtatcaa tagctttgtc
     1081 atattttccc aacgccatga aaatatcccc ttcgaggtac tttattcttt cgaactcttc
     1141 gaaataatgg agggcttttt gtacataaac caatgcaggt tttaaatttc cctgcaaata
     1201 atgaatacag gctattccgt accaggcata ataagtctcg gtatcgagtt tagtagccaa
     1261 tttaaaataa aagagcgcat catcatagtt ttggagcttt tgataacatt cgcctataca
     1321 acaataggca ttcgcatcag gagcctcgtg ttgtaaactt tctctgtaag tttcgatggc
     1381 cttttcatac atgctagcac tggcataagc atttcccttg ttgaaatatg caggcgaata
     1441 ttcggggtct atggcaatgg caaaatcaaa agcctcgatg cttttttcat acagactcag
     1501 cttattgtat aaaattccca gattgaacca gccaggaaca ttatatggat ttttctcaac
     1561 gaacttttga aaataactta cgcctttatc aagattctca gccatgtcgt aaaatgatgc
     1621 cagctcattg atgatggttt cttcggtcga gtcgtattcg agcgcttttt ccaactgtcg
     1681 aatggcttcc tcatattccc agattttttc atgctcgaaa gctaaagtaa ctaatatttc
     1741 ataaggattg tcagtaagtt tcagggcttt tttcaattct ttgatagcta gcttgtgctg
     1801 gcttaatttg ctataaataa aactgcgagt ataatgaatc tcgaaattat tgggaaaaat
     1861 ccgttccaat cgtgaaagaa ttttgagcgc cttctcttcc tgattggtag aagccaaaag
     1921 ttgagctttt ttcagctcga atatgctatt gtaaggatac tgatgctgag ctgcctcaat
     1981 tgctgcctgg gctcgcgggc tatcatgctg ttcgagataa tagtcgatga tgtcctcgaa
     2041 atcttctgca tcaaaaaaat attccacctg ctcctccatc atcttct
//
LOCUS       DAORPD010000261         2087 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_9788_length_2087_cov_1.999508, whole genome shotgun sequence.
ACCESSION   DAORPD010000261 DAORPD010000000
VERSION     DAORPD010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9788_length_2087_cov_1.999508"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="PK976_03890"
     CDS             <1..572
                     /locus_tag="PK976_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HRC67060.1"
                     /translation="ISPTAALSSFPYTPVESMKALKYFYRKLPKTWGYYGFYDAFNQK
                     RNWWATSYLAIDEGPILVMIENYRSALLWNLFMKNPEINPALENIGFVYDPFGIDKIP
                     QRNKYFELTPNPYQETISIRYLSNQPTIASIKIYNSAGQSIPLSEKTLYFNDDSKVKT
                     LNLNSLKKGLYIITIEGNHSKETLKFTKK"
     gene            676..1836
                     /locus_tag="PK976_03895"
     CDS             676..1836
                     /locus_tag="PK976_03895"
                     /inference="COORDINATES: protein motif:HMM:NF012889.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC67061.1"
                     /translation="MKKTLLSLFTMVALSMSMLGQTVTVTFNVNMTNAGLSDQNVYIA
                     GDFGATTWGSWNEPGTNPACLMTDEDGDMIYSITMDLPKGTYPFKFFKGEGWSGDEWP
                     GDPNRTATVKKAMNYSYVWAVRLDQPQILADFEDGTWGILTPHVMGCGDYDNPDLHPL
                     EETFMIVDNPSPDAMNHSAKVLKFIRRGTTNGGLPWGGYWANNNPSVDAQTNKYVHVK
                     LLKPRISPVKFKLEGGTSGTLEIFSENTQQLTNQWEEFVFKFDTMTGQYPISVLMPDF
                     EDPLTITDDIIIYIDDIRIDTIATVSNGIPSISNDKILIYPIPLTKDLTIENFGKIVR
                     VNVYNLAGQLIKQIETQPSSKVVINLEDLCSGMYLIYITNTEGKTITRKISK"
BASE COUNT          698 a          428 c          347 g          614 t
ORIGIN      
        1 ctatttctcc aaccgccgcc ttgagctcat tcccttatac cccggttgaa tccatgaaag
       61 ccttaaaata tttctaccgt aagcttccta aaacatgggg atattatgga ttctatgacg
      121 ccttcaatca aaaacgtaac tggtgggcta cctcctacct ggctattgat gaaggcccaa
      181 tattggtcat gatcgaaaat tatcgttcag ccttactttg gaatctattt atgaaaaatc
      241 ccgagataaa tcctgccctt gaaaatattg gcttcgttta cgaccccttt ggcattgata
      301 aaattcctca aagaaacaaa tacttcgaac taactcccaa tccatatcaa gaaactattt
      361 ccattagata tctttcgaac caacctacga ttgcttcgat caaaatttat aactctgcgg
      421 gacagtcgat tccattatca gaaaaaacac tttatttcaa cgatgattct aaggttaaaa
      481 cccttaattt aaactcatta aagaaaggat tgtatatcat taccatcgaa ggcaatcatt
      541 caaaggagac tcttaaattt accaaaaaat aaaaaaatta ctttttttaa aaaaatttaa
      601 agtaagatgg aattttcgga taaatattgt ttaaatttgc tacgaattat tctgttaaac
      661 ctaaaaacta aaattatgaa gaaaacttta ctttcattat ttacgatggt agctctcagc
      721 atgagtatgc tgggacaaac cgtaacagta acctttaatg taaatatgac caatgctggt
      781 ttgagcgacc aaaatgttta cattgctggc gattttggag caacaacatg gggtagttgg
      841 aatgaaccag ggaccaatcc agcttgctta atgacggatg aagatggtga tatgatttac
      901 tctattacta tggacctccc taaaggtacc tatcctttta aatttttcaa aggtgaaggt
      961 tggagtggtg acgaatggcc aggcgacccc aatcgtacag ctactgttaa aaaagcgatg
     1021 aattactcct atgtttgggc tgttaggtta gaccaacccc aaattttggc cgactttgaa
     1081 gatggaacat ggggaatttt gacccctcac gttatgggtt gcggcgatta cgacaacccc
     1141 gatctccatc ccttagaaga aacattcatg attgttgata atccctctcc cgacgcaatg
     1201 aaccatagcg caaaagtgct gaaattcatc cgtagaggaa caaccaatgg aggattgcct
     1261 tgggggggat actgggcaaa taataatccc agtgtagatg ctcagactaa caaatatgtg
     1321 catgtaaaac tcttgaaacc acgtatctct cctgttaaat tcaaactcga aggtggtaca
     1381 tcaggtacac tcgaaatctt tagtgaaaat actcaacaac ttaccaacca atgggaagaa
     1441 ttcgtattta aatttgatac catgaccggt caatatccca tctccgttct tatgcccgat
     1501 ttcgaagacc ccctcactat tactgacgat atcatcatct acatcgacga tattcgtatc
     1561 gatacgattg ccactgtatc caatggaata ccttctatat cgaatgacaa aatcttaatc
     1621 tatcctattc ccctaacaaa agatcttact atagagaact ttggaaagat tgttcgagtc
     1681 aatgtttata atcttgccgg acaactgatt aaacaaatcg aaacccagcc atcttccaaa
     1741 gtggtcatca atctcgaaga tctatgcagc ggaatgtatc tgatttatat caccaacacc
     1801 gaagggaaaa ccattacaag aaagattagc aaataattag caacaattgt aaaagcattt
     1861 gctttcatct gattatcccc tcaatttgca ttaatcaagt ttcttaactc ttaaatcctt
     1921 ttaatgagaa gaaatatcat gataaaaatg cagaataagg ggattttctt tgaattcatt
     1981 cttttgaatg aaattccaag ccaacatatc tcaaaaatta tgaaatgcat aggaagcaaa
     2041 cgctggaacc catggttatt ttagtacatc atttacaatc gttcatc
//
LOCUS       DAORPD010000262         2083 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_9814_length_2083_cov_1.963511, whole genome shotgun sequence.
ACCESSION   DAORPD010000262 DAORPD010000000
VERSION     DAORPD010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9814_length_2083_cov_1.963511"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="PK976_03900"
     CDS             complement(<1..578)
                     /locus_tag="PK976_03900"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HRC67062.1"
                     /translation="MKNRFISNILFEDTWIGRQLDKLSRRGLCIIHWSKKIVVPGFEG
                     MPLYDVMTFFLKSLTKGNISAKASGIAYSFLAAIFPAIIMLFTIIPFIPIENFQPMLM
                     GLLEDFLPDEIWRVVNKTVEDIITRPRGGLLSVGFIMALYFCSNGITGLTNAFNSSVH
                     AFKTRSWFKQKAINIFLILLYTILLIVAIALI"
     gene            complement(579..1415)
                     /gene="nadC"
                     /locus_tag="PK976_03905"
     CDS             complement(579..1415)
                     /gene="nadC"
                     /locus_tag="PK976_03905"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005809499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HRC67063.1"
                     /translation="MNVEEIIELALREDIGEGDHTTLATVSRRQRAHARLLIKGKGVI
                     CGIDIARRIFQRLDSSMEFTEILHDGAEVEAGEIGFTLYGNARAILMGERTALNFMQR
                     LSGIATQTCQLVKLTEGFPVRLLDTRKTTPLLRQLEKYAVRVGGGVNHRFGLFDMILI
                     KDNHVDYAGGIAQAIRAVNQYLKENHLDLKVEIEVRNLTELNHVLDEGHIHRIMLDNF
                     TPDELKMAIKIIGGRFETEASGGITLQNIREYAATGVDYISVGALTHHIKSLDLSLKA
                     YI"
     gene            complement(1440..1847)
                     /locus_tag="PK976_03910"
     CDS             complement(1440..1847)
                     /locus_tag="PK976_03910"
                     /inference="COORDINATES: protein motif:HMM:NF027349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4783 domain-containing protein"
                     /protein_id="HRC67064.1"
                     /translation="MKILLQKEFLLFLAIFSGFSASVCAQNNQAVIVKAISDGDAHEL
                     VKYFHTMVNLAIPGSEGVYNRNQSEALLQKFFSQNEVLSFKVKHEGRSNEGTRFLIGK
                     MNCPTKIYNLYYLMKEMEGKYWIIQFQLEPFEE"
BASE COUNT          634 a          431 c          389 g          629 t
ORIGIN      
        1 ctgattaggg caatggcaac gatcagcaat atggtatata gaagtatgag gaatatattt
       61 atggcttttt gtttgaacca agaacgtgtt ttaaaagcat gcacactcga attaaaagca
      121 ttggtaagac cggttatgcc attcgagcaa aaatacaagg ccatgataaa ccctacggag
      181 agcaaaccac ctcgtggtcg tgtgatgata tcttcaactg ttttattaac cactcgccag
      241 atttcatcgg gtaagaaatc ttcgagcaat cccatcagca taggctgaaa attctcgata
      301 gggataaaag gaatgatagt aaatagcatt atgattgcag gaaagatagc agctaaaaaa
      361 ctataggcta tacccgaagc cttggcactg atattcccct tcgttagcga tttcaggaag
      421 aatgtcatta catcgtatag aggcattcct tcgaaaccag gtacaactat tttcttcgac
      481 caatgaatga tacaaagccc ccttcggctc agcttatcca attgccggcc aatccaagta
      541 tcttcgaaaa gaatattcga tataaagcgg ttcttcactt atatataagc cttcaaactt
      601 agatcgagac tcttaatatg atgggtaaga gcacctaccg agatgtaatc aacccctgtg
      661 gcagcatatt ctcttatatt ctgcaacgta atgcctccac tggcttctgt ttcaaaacgg
      721 cctccgatta tttttatggc cattttcaat tcatcaggtg taaaattatc gagcataatg
      781 cggtgaatat gcccttcgtc aagtacatgg ttcaattcag ttaggttgcg gacttctatt
      841 tctactttca aatcaagatg gttttctttg agatattgat tcactgctct gatagcctgt
      901 gcaattcctc cggcataatc cacatggttg tccttgataa ggatcatgtc gaaaagtcca
      961 aaacgatgat tgactcctcc tccaacccgt acagcatatt tttcgagttg acgaagtaat
     1021 ggggtagttt tgcgcgtatc gagcaatcga accgggaagc cttcggtaag ttttaccagt
     1081 tgacaggttt gagtggctat gccgctcaat cgttgcataa aattaagagc agtcctttca
     1141 cccataagta tagccctggc attgccataa agcgtaaaac caatttctcc ggcttctact
     1201 tcggctccgt cgtgcaaaat ttcagtaaat tccatggagg aatccaagcg ctgaaatatt
     1261 cttcgggcaa tatctatccc gcagatgact ccctttccct ttatcagtaa tcgagcatgc
     1321 gctctttgtc gtcgagatac ggttgcaagg gtggtatgat caccttcacc tatatcttct
     1381 cgaagggcta attcaataat ctcttctaca ttcattttaa tttcaatgtt tcttgagaat
     1441 cattcttcga atggttctaa ttggaactgg ataatccagt atttcccctc catctccttc
     1501 attaaataat acagattata aattttagta gggcaattca ttttgccaat aagaaaacgt
     1561 gttccttcgt tcgatcgtcc ttcgtgcttt accttgaatg ataatacttc attctgactg
     1621 aagaatttct gcaaaagtgc ttccgactga ttacggttat atacaccttc gcttccggga
     1681 atggctaaat tgaccatagt atgaaaatac tttaccaatt catgtgcatc gccatccgaa
     1741 atagctttta caatcacagc ctggttattc tgggcacaaa cagaagccga aaagcctgaa
     1801 aaaatcgcca gaaacagcaa aaattctttt tgtaataata tcttcatgcc cctttcgaat
     1861 taatgaataa tgaaagaaat tctgaaacgg tttatattac aataaaagtg ccataagcat
     1921 tggttgcgaa aatgagcaaa tttatggtaa aatatgatta ccatgaggaa ttcaatacaa
     1981 taaggtcgaa aaatcataat ttcgcacttg ctctaaattt tacttatgaa gattaactta
     2041 atagcgatcg ggaaaactga tgaggcctat gtggaagatg gtt
//
LOCUS       DAORPD010000263         2081 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_9830_length_2081_cov_2.098717, whole genome shotgun sequence.
ACCESSION   DAORPD010000263 DAORPD010000000
VERSION     DAORPD010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9830_length_2081_cov_2.098717"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="PK976_03915"
     CDS             complement(<1..292)
                     /locus_tag="PK976_03915"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRC67065.1"
                     /translation="MKRLLLFMVILLTTSSFILAQNRIEGKVTDAKSGEALPGVTIYI
                     KGTTIGTVSDVNGKYVLESAALKPQAVIVFSFVGYNTQEVVFQNQKVLDIKLE"
     gene            complement(307..2043)
                     /locus_tag="PK976_03920"
     CDS             complement(307..2043)
                     /locus_tag="PK976_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67066.1"
                     /translation="MKKLKTIYFGNSCIALLLIVLTIACTQQKPAPLLQKSIVRIEQM
                     PDLPQPLSIIDWGEKARQFDSLVFDFEATTPFAPYIWWDTNHRNFPQNTYGIYTAIGD
                     ARQGSSKNGGEFHEALVTLPALLSAGLIGIDKTHQNGYNYVELAQNYFNRDNGWNIVM
                     NNTHPDVAHLGGGYGRDWWYDVYPNVLYYSVAALFPHVQRNDEIMRSVANKFYQADSI
                     LNGCYDYSYFDYGRMKPMRNNIPFQQDAAGGHAWVLYSAYKKFGDTKYLQGAISACKA
                     LDNQKESRFYEILLPFGAYTAARLNAEQGQNFDVEKILNHIFDGCQSTDGRYGWGVIA
                     EKWGDYDVYGLQGSIIDGGGYAFLMNSVVMAWPLLPMVKYDPQYARAIAKYLLNAINA
                     ARLFYPDQMDEQHQWLSNKKDITKGIIAYEGLRKHDDYGKPELKGITPVALGDGPKWA
                     PGQPEESMFSLYSSAVAGIYGAIVNKTDIEGILRLNCNATDFYGDNDFPVYLIYNPYL
                     ENREINYSIDTASDLFDILSRNYICRNGEGAVKLEIPAHTALLIAELPSMSKIKFSNH
                     KIWCNDKTVSWK"
BASE COUNT          594 a          445 c          421 g          621 t
ORIGIN      
        1 gttccagctt aatatcgaga accttctgat tctggaagac aacctcctga gtgttgtatc
       61 caacaaaaga gaaaacaatc actgcttgag gcttcagagc ggccgattcc aaaacgtatt
      121 taccgttaac atccgatact gtgccaattg tggttccttt gatgtagatg gttacacccg
      181 gtaaggcctc accacttttg gcatccgtca ctttcccttc tatacggttt tgggccagaa
      241 taaaagaaga agttgttaac aaaatgacca tgaaaagtaa tagtcgtttc ataagaatag
      301 atttttttat ttccagctaa cagttttgtc gttacaccat attttatgat ttgagaattt
      361 aatcttcgac atagaaggca actctgcaat caaaagagca gtgtgtgcag gaatttctaa
      421 tttgacagca ccctcgccat ttctgcaaat ataatttcga gagagaatat caaaaagatc
      481 agaagcagta tctattgaat aatttatttc acgattttca agatatgggt tatagatcag
      541 ataaaccggg aaatcattgt ctccataaaa gtcggtagca ttgcagttaa gacgtaatat
      601 accttctatg tcggttttgt tgacgatagc cccataaatt ccagccacag ccgaactata
      661 gaggctaaac atggattctt cgggttgacc tggtgcccat ttgggcccat ctccaagggc
      721 tacgggagta attcccttga gttcaggttt cccatagtca tcgtgcttgc gtagcccttc
      781 ataagcgatg attcctttag taatgtcttt tttattgctg agccattgat gttgctcatc
      841 catctgatcg ggataaaaaa gacgagcagc attgattgca ttgagcagat attttgctat
      901 agcccttgcg tactgaggat cgtattttac cataggaagc agaggccatg ccatgaccac
      961 agaattcatc aaaaaagcat accctcctcc atcgatgata ctgccctgaa gtccatatac
     1021 atcgtaatcg ccccattttt cagcaattac accccatcca tatcttccat cggtagattg
     1081 gcagccatcg aaaatatgat tgagaatttt ttcgacatcg aaattttgac cttgttctgc
     1141 attgagacga gcggccgtgt aagctccaaa tggaagaagg atttcgtaaa acctgctttc
     1201 tttctgatta tcgagagctt tgcatgcact tatggcacct tgaagatatt tcgtatctcc
     1261 gaatttcttg taagccgaat agagtaccca tgcatggcca ccggctgcat cctgctgaaa
     1321 tgggatattg ttacgcatgg gtttcatcct tccataatca aagtaggaat aatcgtaaca
     1381 tccgttcaaa atgctatcgg cctgataaaa tttattggcc acacttctca ttatctcgtc
     1441 gtttctctga acatgaggga aaagtgcagc aacgctataa tacaagacat ttgggtaaac
     1501 atcgtaccac caatccctgc catagcctcc acctagatga gccacgtcgg gatgggtgtt
     1561 gttcatcact atgttccatc cgttatcgcg attgaagtaa ttttgcgcca attcaacgta
     1621 attatagcca ttctgatggg ttttgtcgat tccaataagg ccggcactca ataaggctgg
     1681 aagtgtaaca agtgcttcgt gaaactcacc tccatttttc gatgagccct gacgagcatc
     1741 tcctatagcc gtatatattc cgtaagtatt ctgaggaaaa ttccgatggt tcgtatccca
     1801 ccagatataa ggagcaaacg gagtggtagc ctcgaaatcg aagacaagac tatcgaattg
     1861 tctggctttt tctccccaat cgataatgga aagaggttgt ggcaaatcgg gcatctgttc
     1921 aattcttaca atgctttttt ggagcaaagg tgcaggcttc tgctgtgtac aagcaatggt
     1981 cagaactatg agcaacagag cgatacagga atttccaaaa taaatcgttt ttaatttctt
     2041 cataggggtt gataagattt tttcatgatt gttttagcca g
//
LOCUS       DAORPD010000264         2071 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_9901_length_2071_cov_2.615575, whole genome shotgun sequence.
ACCESSION   DAORPD010000264 DAORPD010000000
VERSION     DAORPD010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9901_length_2071_cov_2.615575"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="PK976_03925"
     CDS             complement(<1..251)
                     /locus_tag="PK976_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HRC67067.1"
                     /translation="MEEYQNQTEVKDDIVIRFSGDSGDGMQLTGTLFSETAAFTGNDL
                     LTFPDFPAEIRAPQNTLAGVSGFQLHFGSKKIYNSGDLC"
     gene            complement(654..1235)
                     /locus_tag="PK976_03930"
     CDS             complement(654..1235)
                     /locus_tag="PK976_03930"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRC67068.1"
                     /translation="MKKLILLTSFVIISFLCVNAQMKINPSVGIGIAVPMGDFGDAVG
                     LGYGASVGADFKIKPHIKPELIASLGYYMFSSKEQKYTKSSMTGGEYGFKTKANTFYI
                     GAGAKYVTLSGFYGAALISFHNFTNKVEYNYPDYLPLEDETISKDFTRLGFTPIVGYQ
                     FKLAALKLEVYVRYSIISDYNNFGAGASLKFSL"
     gene            1798..1968
                     /locus_tag="PK976_03935"
     CDS             1798..1968
                     /locus_tag="PK976_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRC67069.1"
                     /translation="MAYVITEDCIACGTCIDECPVEAISEGDIYKIDPDMCTDCGSCA
                     EVCPTEAIHPAE"
BASE COUNT          676 a          363 c          371 g          661 t
ORIGIN      
        1 tcacaaagat cacccgaatt ataaatcttc ttcgaaccaa aatgtaactg aaaacccgaa
       61 actcccgcaa gagtgttttg aggtgccctg atctcggccg gaaaatccgg aaaagttaaa
      121 agatcattgc ccgtaaacgc cgcagtctcc gaaaataaag tacccgtcaa ctgcatacca
      181 tcaccagaat caccggaaaa acgtattact atatcgtctt ttacctcggt ttgattttga
      241 tactcttcca tatacatgac ttttgaatgt tgatgcaaag gtatggctta tttatcaatt
      301 gccaattaca atttatttcg taatttgtta tgagtctctt aacagagaat cgaactctcc
      361 gaggagcctg ttaaaatatc tcattttata tgtattttac aagaaacgga aatgtcttgt
      421 aaacgaagat aaataattca aaatagaaag ggaataatcc atttttttcg aatgtgtata
      481 gcaaaaaaac cagctcgata tttagctcgg aggaacgaac caaaaaccga agctggcttt
      541 tgaaattgaa tatgcttact taattgagtt taaagcttga acaaaaccat acctgaaatc
      601 tgttgaaaat tagaagggtt ataacaacaa aggttgttgt taatgattta gacttagagt
      661 gaaaatttca aagatgcacc tgcgccgaag ttgttgtagt cggatatgat ggaatatcta
      721 acataaactt cgagttttaa ggcagcaagc ttgaactgat agcccacaat gggggtgaaa
      781 cccaaacgtg tgaaatcttt acttatggtt tcatcttcca aaggtaggta gtcggggtag
      841 ttatactcca ccttgttggt aaaattatga aacgaaatca gtgcagcacc ataaaaacca
      901 cttagagtta catatttggc tcctgctcca atataaaacg tgtttgcttt cgttttgaag
      961 ccatattctc cacctgtcat tgagctttta gtgtactttt gttctttcga agagaacatg
     1021 tagtaaccca agctggcaat cagttcaggc ttgatatgcg gtttaatttt aaagtcggcc
     1081 cctacggaag cgccataacc taaacctaca gcatcaccaa aatcacccat ggggacggca
     1141 atacctattc ctactgatgg attaatcttc atttgtgcat ttacacacaa aaatgaaatg
     1201 attacaaagc tggttaatag aattaatttt ttcatagtaa ttgattttta aatttttaag
     1261 ttattgattt agatgaatga aacgagaaag atggttgttt tttaaattta tatggttcta
     1321 ttcatttgct ttaaactctt tgaagatttt tttaagtcgc atcataccct tcttcccttc
     1381 agggtggcaa ggagtattga taagacttgt cattgataga acactcagat ggttttagtg
     1441 ttattcttat catttttcag gatgagttta attcaataca aatgtatgta aacaaatcac
     1501 gagatggcaa aattttacaa tgaaaggtaa aatttatttt gtggtttctt cttaatatac
     1561 atatctttat cactattaac gcattataca tttaaaccca gcatatttaa actcaaattg
     1621 ccccaatcga tgacctatag ctgttgcgta tcgttgcaat aaatcgacta ttccattttt
     1681 agtcggtaat tatgtacgaa tataggtatt ttttagaatc aaaataaatt cagagagata
     1741 aatgagtatt aaatgaaaag atataatttt gccaagtcaa ttgaaaaatt taaaatcatg
     1801 gcttacgtaa ttactgaaga ttgtattgcc tgtggcacgt gcattgacga atgccccgtc
     1861 gaagcaatca gcgaaggtga tatttacaaa atcgatcccg acatgtgcac cgattgtggt
     1921 agttgtgccg aggtttgccc aacagaagcg attcatccgg ctgaataact ggcaatttaa
     1981 aatttcaatc aggaaccacc gtactcttgc ggtggttttt ttttatgacg ggcatacatt
     2041 actacttgca agatgaaagt tcttgtacac g
//
LOCUS       DAORPD010000265         2070 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_9910_length_2070_cov_2.267494, whole genome shotgun sequence.
ACCESSION   DAORPD010000265 DAORPD010000000
VERSION     DAORPD010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9910_length_2070_cov_2.267494"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="PK976_03940"
     CDS             <1..283
                     /locus_tag="PK976_03940"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC67070.1"
                     /translation="WIRVDPSLHTRNYPDFSFRYFPNPVQNFVIFESDRNISRIELIS
                     MDGKIVKLIEVVPSPQIKIKLNDLPTGLYVFKLYDSEELIRLGKLIKRQ"
     gene            442..>2070
                     /locus_tag="PK976_03945"
     CDS             442..>2070
                     /locus_tag="PK976_03945"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HRC67071.1"
                     /translation="MRKFYLILAECLMFSMVAKTQVIEHSYSWNNPIIKDISGYSSLF
                     LAGCQIAGEAGAPGLPYQAVKLLLPPAHEADSMEVEYSQPFEIHLASSILPMQHAKPY
                     SLAGNSKWLQNEKIYSSSKPYPNSKQGKLSTHFFKGLSIALSTLTPVEYFPKEQKVIV
                     YQHAKVKIFCHETHKARKALENLYPVSFSDKDLKNLIQNASEIHQYASDKKLNNPYDL
                     LILTKESFTEYFFPLASLYEQNGIAVRIDTLENVLTNTAGQDDQEKIRNFIIAQYQQN
                     GISYVLLGGDVEIFPARGFYCKVFSSSIYEDNNIPSDLYYSALDGTWNDDGDDLWGEI
                     GEDDLLPEVAVARLPFSTTTELQNMLHKIISYQSNPIPNELEKPLMAGELLYNNPLTF
                     GEDYLKQLIGYHEDNGYITNGIPEYQEIDSLYESHLGYWSKYDLLQKINTGKSFIHHA
                     GHANWDYAMKLSSSDINNQNFSQVNGIDHNYTLVYTHGCICGAFDYSDCIAEEMLKIE
                     NFAVAGAFNSRYGWFNEGQTEGPSLHLHREFVNAL"
BASE COUNT          648 a          433 c          368 g          621 t
ORIGIN      
        1 ctggataagg gtcgacccct ctttacacac aaggaattat cccgatttct cattccgata
       61 tttcccaaat cccgttcaaa atttcgttat attcgaatct gaccggaaca tttcaagaat
      121 cgaactcatc tcgatggatg gcaaaatagt aaagctaata gaagttgttc cttcgccaca
      181 gataaaaatt aagttaaatg acttgccaac aggtttatac gttttcaaat tatacgacag
      241 cgaagaattg attcgcttgg gtaaacttat aaaacgtcaa taaccagggg cctttcgagc
      301 ccctaaactt ttttgatcga aatttactca ttctttttcc caattaaatg ctgagggttt
      361 aaaaatttta tgaactttgc atgtgtcttt aatgattttt aagcaattat taaaacaagt
      421 aattatttat atccaatgtc tatgagaaag ttttacttga tcttggccga atgccttatg
      481 ttctcgatgg ttgctaaaac acaagtaatc gagcattctt actcctggaa taatcccatt
      541 attaaagata tttcaggata ttcatctttg tttctggctg gttgtcaaat agctggagaa
      601 gctggtgcac ccggacttcc ctatcaggcg gtgaagttat tgctgcctcc agcccatgaa
      661 gccgattcaa tggaggtgga atattcccag cctttcgaaa ttcatcttgc ttcatccatc
      721 cttcctatgc aacacgcaaa gccttattcc ttggcgggaa attcaaaatg gctccaaaac
      781 gaaaaaatct attcgtcttc aaaaccctat cccaacagca aacaaggaaa actcagcact
      841 cattttttta aaggcttgtc tattgctctt tccactttga ctccagtaga atattttcca
      901 aaagagcaaa aagtgattgt ctatcaacat gcaaaagtca agatcttttg tcacgaaacc
      961 cataaagcca gaaaagcttt agaaaacctt taccctgtct cattttctga taaagatttg
     1021 aaaaacctga tccaaaatgc ttctgaaata catcaatacg cttcggataa aaagttgaac
     1081 aacccttacg acctgctaat actcaccaaa gaatctttca ccgaatattt cttcccattg
     1141 gcttctcttt acgaacaaaa cggaatagcg gtgagaatcg atactctcga aaacgttctg
     1201 accaatacgg caggtcaaga cgatcaggaa aaaatccgaa atttcatcat tgctcaatac
     1261 cagcaaaatg gaatatcata cgtcttattg ggaggtgatg tagagatttt tccagcaaga
     1321 ggattttact gtaaggtttt ttcatcttca atctatgaag acaacaatat tccttctgac
     1381 ctctattatt cagccttgga tggaacttgg aatgacgatg gagacgacct ttggggtgaa
     1441 ataggagaag atgatttatt gccagaggtg gccgttgccc gccttccttt cagtactacc
     1501 accgaacttc agaatatgct tcataaaatc attagctatc aatcgaaccc tatccccaat
     1561 gaactcgaaa aacctctgat ggctggcgaa ctgctttaca ataatccttt gacttttgga
     1621 gaagattacc tgaaacagct gattggatat catgaagata acggctatat cactaatggt
     1681 atacctgaat atcaggaaat agattcattg tatgaaagtc atttgggtta ctggtcgaaa
     1741 tatgatttac tgcaaaagat caacactgga aaatcattta tccatcatgc tggccatgca
     1801 aactgggatt atgccatgaa actttcttcg agcgatataa acaaccagaa tttttctcag
     1861 gtgaatggga tcgaccataa ttatactttg gtttatacac atggatgtat ctgtggagca
     1921 ttcgattatt ccgactgcat tgcagaagaa atgctgaaaa tcgaaaattt tgcagttgcc
     1981 ggggcattta attcgagata tggctggttt aacgaaggcc aaaccgaagg cccctccctc
     2041 catcttcacc gtgagtttgt caatgccctt
//
LOCUS       DAORPD010000266         2064 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_9943_length_2064_cov_2.811349, whole genome shotgun sequence.
ACCESSION   DAORPD010000266 DAORPD010000000
VERSION     DAORPD010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9943_length_2064_cov_2.811349"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..879
                     /locus_tag="PK976_03950"
     CDS             145..879
                     /locus_tag="PK976_03950"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HRC67072.1"
                     /translation="MLNIAIDIGNSMTKVGYFENDQLVHFEKVRKLTADRLKGITNRY
                     FSNSPEAVILSNVKEHDIGLYRFLSQTYRFYEVGFNTQYPIKIAYKTPHTLGNDRIAA
                     AAAAYSKSSGAPALVITCGSCIIYDFVDENGNYLGGAISPGLRMRLKALHNFSAKLPL
                     IKPRFIDSNLGVDTETSILSGVINGMTYEVDGFIDEFRTKNIDLSVFLSGGDMLYFEG
                     KFKNSIFAVPNIVLEGLNKILLFNER"
     gene            869..>2064
                     /locus_tag="PK976_03955"
     CDS             869..>2064
                     /locus_tag="PK976_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67073.1"
                     /translation="MSVNRKILFVLPLIMMACLSKKSLSQSSITSPYSHYGIGDLSSI
                     SNTRNRTMGSIGYAQRGFYNVNPINPASYTSYDSMSFVFEGGMKSSFVTLKTSGISES
                     ATSASLDFLFFGFPVSRRIGVSFGTMPFSTVGYKILQKQTDPMLGEVNYYYQGDGGLN
                     QYYIGAAFKLHKNFSIGFNAIYLAGNLERSRLLHFPDSVAILNTRIKDNIHISDISYS
                     AGIQYHRTLSKGNLFNLGATVSYGNKLNTNTDKLSETLFGGINGGYELYKDTIEYISD
                     IEGDLKLPLHFGGGFLYEIPEKLCFGADFMYSFWEDYTISGKSDSLKNSYRINAGVEW
                     IPDNRPISKYWQKIRYRFGINYNDTYLRLNGTNINEFTASIGFGLPLRRQAASINFGA
                     EFGNRGT"
BASE COUNT          647 a          338 c          420 g          659 t
ORIGIN      
        1 tcagctgaag acagcgacgc ggctctaccg actgagctac gacaccatca tttagttcga
       61 ggctgcaaag ataaatattt tgtgtaatca accaaaatat tctcaaggtt tcaatacgat
      121 tgttataact ttgcataaaa gctgatgtta aatatagcta tagacatagg aaattctatg
      181 acaaaagttg gttatttcga gaatgatcaa cttgtgcatt ttgaaaaagt aaggaagtta
      241 actgccgacc gtttaaaagg aatcaccaat agatactttt caaactctcc tgaagctgtt
      301 atcctatcga atgtaaaaga acacgacatc gggttatata gatttttgag tcaaacctat
      361 agattctatg aagtaggttt taatactcaa tatccgatta agattgcgta taaaactcca
      421 catacattgg gaaatgatag aatagctgcg gctgcggctg catattcgaa atcttcgggt
      481 gcccctgcat tggttatcac atgtggaagc tgtattattt atgattttgt cgatgaaaat
      541 ggaaactatc tgggaggtgc aatttcgccg ggtttaagaa tgagattgaa agctttacat
      601 aattttagcg caaaattacc gttgataaaa ccgcgtttca ttgattcgaa cttaggtgtt
      661 gataccgaaa catcgatact ctcaggggta atcaatggaa tgacttatga ggtagatgga
      721 tttatcgacg aatttcgcac aaaaaacatt gatttgtctg tatttttaag tggaggagat
      781 atgttgtatt ttgaaggtaa gtttaaaaat agcatttttg cagttccaaa tattgtgctt
      841 gaaggactta ataaaatttt gctttttaat gagcgttaat agaaagatat tgtttgttct
      901 tcctctaatc atgatggctt gtctctcaaa gaaaagcctt tcacaatcga gtattacttc
      961 cccctattcg cattatggca taggtgactt gtcaagcata agtaatacga gaaatagaac
     1021 gatgggttca attggctacg cccaaagagg attttacaat gtgaatccca taaatcccgc
     1081 ttcttatact tcttatgatt cgatgtcgtt tgtctttgag ggtggcatga aaagttcctt
     1141 cgtaactttg aaaacatctg gaatttcgga gagcgctacg agtgcaagct tagacttctt
     1201 gttctttggt tttcctgttt ctcgaagaat tggagtaagt ttcggaacca tgccattttc
     1261 gaccgttggt tataaaattc tccaaaaaca aacagatccc atgttaggag aggtcaatta
     1321 ttattaccaa ggagatgggg gtcttaatca gtactatata ggagctgcat ttaagttaca
     1381 caaaaatttt tctattggct tcaatgcaat ctatttagcg ggcaatttag aaaggagccg
     1441 cttattacat ttccctgatt cggtagctat attgaacacc aggattaaag ataacattca
     1501 tattagtgat atttcttact ctgctggtat tcaatatcat agaactttat cgaaaggaaa
     1561 tttgttcaat ctgggtgcaa cagtcagtta tggaaataaa cttaacacca ataccgacaa
     1621 actctcggaa actttgtttg ggggtattaa tggaggctat gaactctata aggatacaat
     1681 agaatatata tcggatatag agggcgattt aaaattgcct cttcattttg gaggtggatt
     1741 tttatacgaa attcccgaaa agctatgttt tggcgctgat tttatgtatt cgttctggga
     1801 agattacact atatctggga agtccgattc attgaaaaat agttaccgaa ttaatgcagg
     1861 tgtggaatgg atccccgaca atagaccaat atcgaaatac tggcaaaaaa tcaggtatcg
     1921 ttttgggatt aattataacg atacgtattt gagactcaac ggaaccaata ttaatgaatt
     1981 tactgccagt attggctttg gactcccttt gagacggcag gcagcctcga ttaattttgg
     2041 cgcagaattt ggtaacagag gtac
//
LOCUS       DAORPD010000267         2063 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_9953_length_2063_cov_2.458665, whole genome shotgun sequence.
ACCESSION   DAORPD010000267 DAORPD010000000
VERSION     DAORPD010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9953_length_2063_cov_2.458665"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1038)
                     /locus_tag="PK976_03960"
     CDS             complement(<1..1038)
                     /locus_tag="PK976_03960"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HRC67074.1"
                     /translation="MSSKTLFDKIWDDHVVYSIEGGPDVLYIDMHYIHEVTSPQAFNG
                     LRNRGIKVFRPERTLATADHNIPTKDQHLPIRDEISRNQVETLEKNCREFGIKFFGMQ
                     HPNNGIVHVIGPELGFTRPGMTIVCGDSHTSTHGAFGSIAFGIGTSEVEMVLASQCIL
                     QNRPRVMRISVNGTLPHATTAKDIIMFIISRLSSGGATGYFIEYAGQAIESLSMEGRM
                     TVCNMSIEMGARGGLIAPDNKTIDYVKKSSKISGIEITENEIRNWENLKSDNGASFDR
                     EVEFDAGEIKPMITFGTNPGMAIAVDDVIPEPSENKKEATSFMKALEYMGFKPGMKLE
                     GHKIDYVFLGSC"
     gene            complement(1052..>2063)
                     /locus_tag="PK976_03965"
     CDS             complement(1052..>2063)
                     /locus_tag="PK976_03965"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010539074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HRC67075.1"
                     /translation="GATVVNIPDTTGYCLPVEYGEKIKYLKDNVPNIDKAIISTHCHN
                     DLGMATANTIMGILNGARQAEVTINGVGERAGNTSLEEVVMTLKSHKNLKFVTGINTQ
                     LIYKTSRLVSTLMNMPVQPNKAIVGRNAFAHSSGIHQDGVLKKRENYEIINPVEVGVR
                     ESSIILSARSGRAALNHRLELLGYKFNKEELDDIYYRFLKLADKKKIINDDDIKILTG
                     EVTQGEKPLRLELLQVSCGKSLIPVATVGLWINGEIHTATATGNGPIDAAFTAVRQLI
                     NKTVHLEEFLIQAITRGSDDIGKVHIQVEHQGKIYYGFSANTDIITASVEALIDAIAK
                     IK"
BASE COUNT          495 a          434 c          444 g          690 t
ORIGIN      
        1 acaactaccg aggaaaacat aatctatttt atgtccctcg agtttcatgc ctggtttgaa
       61 gcccatatat tcaagagctt tcataaatga tgttgcttct tttttatttt cggaaggttc
      121 aggaatgaca tcatcaacag ctattgccat acccggattt gttccgaatg ttatcatagg
      181 ttttatttcg cctgcgtcga attccacttc cctgtcaaaa ctggctccat tgtccgattt
      241 aagattttcc cagtttctta tttcattttc cgtgatttca atacctgaga ttttggagct
      301 tttcttaacg taatcgattg ttttgttatc gggtgcaata aggcccccgc gggctcccat
      361 ttcaatactc atattacaca cggtcattct accttccata ctgaggcttt cgatagcctg
      421 tccggcatat tctataaaat agcctgtagc tccgccggaa gaaaggcgcg agatgataaa
      481 cattataata tctttggcag tggttgcatg tggaagtgta ccatttacac ttattctcat
      541 cacccgtggt cgattttgga gtatgcattg ggaagcaagt accatttcca cctcggaagt
      601 gccgattccg aaagcaatcg aaccaaaggc accgtgagtt gaggtatggc tgtcgccgca
      661 tacaatcgtc ataccaggtc gtgtaaaacc aagttctgga ccaataacat ggacgattcc
      721 attattagga tgttgcattc cgaaaaactt tattccgaac tccctgcagt ttttctcgag
      781 agtttcgacc tggtttcttg aaatttcatc tcgtattggc aggtgctgat cttttgtagg
      841 aatattatga tcggcagttg ctagagttct ttcaggtcgg aatactttta tgcctctatt
      901 tctgagtccg ttaaaggctt gagggctagt cacttcgtga atgtaatgca tatcgatata
      961 gaggacgtcg ggtcctcctt cgattgaata gacgacgtgg tcatcccata ttttatcaaa
     1021 aagagttttt gaactcataa ttgaaaatgg tttacttaat tttagctatt gcgtcaatta
     1081 aagcttcaac cgaggctgta atgatgtcag tattagcaga gaatccgtaa tatattttcc
     1141 cttgatgttc aacctgtata tgtacctttc cgatatcatc actacccctt gtaatagcct
     1201 gaataaggaa ttcttcgaga tgtaccgttt tattaataag ttgtcgtaca gcagtaaatg
     1261 cggcgtctat agggccattc cctgttgcag tggccgtatg aatttctcca tttatccaga
     1321 gtccaactgt agcaacaggt attaatgatt tgccacatga cacttgcagg agctcgagtc
     1381 gcagaggttt ttcaccctga gtcacctctc cagtaagtat cttaatgtca tcatcgttta
     1441 taattttttt cttgtcggcg agtttaagga agcggtagta tatatcgtca agctcttcct
     1501 tgttgaattt gtatccaaga agttcgagtc tgtggttaag agctgctcgt ccgctgcgtg
     1561 cggagaggat gatagatgat tctcttacgc cgacctcaac aggattgata atttcgtaat
     1621 tttctctttt cttcagtacg ccgtcctggt ggatacctga ggaatgggcg aatgcattac
     1681 ggccgacaat tgctttgttt ggctggacag gcatattcat caatgttgag acgagacggc
     1741 tggttttgta tatgagctgt gtgttgatgc cggttacaaa ttttagattt ttgtggcttt
     1801 tcaacgtcat tacgacctct tcgagggagg tgttgcctgc tctttcgccc acgccgttta
     1861 ttgtaacctc ggcctggcgt gccccgttca gaattcccat gattgtattt gcagttgcca
     1921 ttccgaggtc gttatggcag tgtgtggaaa tgatggcctt atctatgttt ggtacattgt
     1981 ccttcaggta tttgattttt tccccgtatt caacagggag gcaatatcct gtagtatccg
     2041 gtatatttac tacagtggca cct
//
LOCUS       DAORPD010000268         2063 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_9958_length_2063_cov_1.602590, whole genome shotgun sequence.
ACCESSION   DAORPD010000268 DAORPD010000000
VERSION     DAORPD010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9958_length_2063_cov_1.602590"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1094
                     /locus_tag="PK976_03970"
     CDS             <1..1094
                     /locus_tag="PK976_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HRC67076.1"
                     /translation="IITASHNPANWNALKLLNRDGEFISSDEGEEILRISEEESFIYA
                     ELADIGRYSQNDEMLSHHIRRILQLPLVDVEAICRANFKIVVDTVNSSGGIAIPSLLR
                     ALGVKEIICLNCTPDGNFAHNPEPLPEHLGEISEAVKKHGAHLGIVVDPDVDRLAFVC
                     ENGEMFGEEYTLVAVADYVLQHRGGPTVSNLSSTRTLKVITQKAGYEYFASAVGEVNV
                     VELMKKTHAVIGGEGNGGIIYPELHYGRDALVGVALFLSYLAHTGLTASSLRKSYPNF
                     FMSKNKIDLPENTDIRSILEEISKKYSSFPQNKVDGLKIEFPDEWVHLRRSNTEPIIR
                     IYAESLSPDKAEELVKKFIYEIEQFIPIL"
     gene            1204..1440
                     /locus_tag="PK976_03975"
     CDS             1204..1440
                     /locus_tag="PK976_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67077.1"
                     /translation="MPKKKKRKPLKSKKLIIKLGASQMELLNNFCIRHQTTPNKVIKQ
                     SLMTYLNNCKPEDLKPLEEVDPRQLDIFTMLDNN"
     gene            complement(1449..>2063)
                     /gene="recR"
                     /locus_tag="PK976_03980"
     CDS             complement(1449..>2063)
                     /gene="recR"
                     /locus_tag="PK976_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HRC67078.1"
                     /translation="PSKYLEAAVNELSKLPGIGRKTALRLCLYLLDQPQAFAQNLGEA
                     LIRLRENIRYCEQCHNISDEPVCRICSDGFRDSSLVCIVESARDVMAIENTGQFKGIY
                     HVLGGIISPFEGKGPTDLTLAPLIAKAESGTIKEIIFALPANMEGDTTCFYLYKKLKP
                     FVDTFTTIARGIAIGEELEYTDELTLGRSIMNRTSYEMPLNNKN"
BASE COUNT          643 a          447 c          416 g          557 t
ORIGIN      
        1 tcatcattac tgccagccat aatcctgcga actggaatgc tttgaagtta ttgaaccgag
       61 atggagaatt catatcatct gatgaaggag aagaaatatt aaggatttcg gaagaagaat
      121 ctttcatata tgccgaattg gccgatattg gccgatattc acaaaatgat gaaatgctgt
      181 cgcatcacat tcgccgaatt ctgcaacttc ccttagtgga tgtagaagcc atttgcaggg
      241 caaatttcaa gattgttgta gatacagtca attcatcagg gggaattgcc attccgtcat
      301 tgttaagagc tcttggggta aaggaaatca tctgtttaaa ctgtactccc gacggtaatt
      361 ttgcacataa tcccgaaccc ttgccagagc atctgggtga aatttccgag gcagtaaaaa
      421 aacatggtgc gcatctgggc atagtagtcg atcccgacgt cgatcgtctg gcttttgttt
      481 gtgaaaatgg agaaatgttt ggcgaagaat atacattggt ggctgtagcc gattatgtac
      541 ttcagcatcg tggaggtcct acggtttcga atctttcctc taccagaacc ttgaaggtga
      601 ttactcaaaa ggcaggttac gaatattttg cttcggctgt tggcgaagta aatgtggtgg
      661 aattgatgaa aaaaactcat gcagtcatag gaggcgaagg taacggcgga atcatctatc
      721 ccgagctgca ttacggtcgt gatgctctgg ttggggttgc cctattcttg agctatctgg
      781 cacacacagg gcttaccgct tcgagcttgc gcaaatctta tcctaacttc tttatgtcga
      841 aaaataagat cgatttgccc gaaaacaccg atattcgatc cattctcgaa gaaatatcca
      901 aaaaatattc ttcttttcca caaaataaag tggatggact caaaatagaa tttcccgacg
      961 aatgggtgca tttgcgacgc tccaataccg aacccattat tagaatatat gccgagagct
     1021 tatcgccaga caaagccgaa gaactcgtaa aaaaatttat atacgaaatc gaacaattca
     1081 taccgatttt atagataaac gcattcgaac tatacttgac ttatgccata cctatagatt
     1141 tatcgaaaac aaataccatc cctcggatta cctgactaac ttattgaaaa cgtttgcata
     1201 gccatgccca aaaagaaaaa acgaaaacca ctaaaatcga aaaaactaat catcaagtta
     1261 ggtgcttctc aaatggaact tctcaataat ttttgcattc ggcatcaaac cacacccaac
     1321 aaagtgatca aacaaagcct aatgacctac ctcaataatt gtaaacctga ggatttaaag
     1381 cccttagaag aagtcgatcc ccgtcagctc gatatattta caatgctcga taataactga
     1441 taaaaacctc aattcttatt gttcaatggc atttcgtaag aagtgcggtt catgatggag
     1501 cgcccaagtg tcaattcgtc ggtatattcg agttcttcac ctatggcaat gccgcgggca
     1561 atagttgtaa aagtatcaac aaaaggtttg agtttcttgt aaagataaaa gcaagtagta
     1621 tcaccctcca tattggccgg cagggcaaag atgatttctt ttattgtccc cgattcggct
     1681 ttggctatca gaggagccag cgtaagatca gtaggacctt tgccttcgaa aggtgatatg
     1741 attcccccca acacatgata aattcctttg aactgacctg tattttcgat agccatcaca
     1801 tcacgggccg attcgacgat acacaccaaa ctgctgtcgc gaaagccatc agaacaaatc
     1861 ctgcaaaccg gctcatcgct aatgttgtga cattgctcgc aataacgaat attttcccgc
     1921 aatcgaatga gtgcttcacc caagttttgt gcaaaagcct gaggttggtc gagcaaatac
     1981 aaacacaaac gcaaagcagt tttacgacct atacccggca gtttcgacag ctcatttacc
     2041 gcagcttcca aatacttcga agg
//
LOCUS       DAORPD010000269         2062 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_9962_length_2062_cov_2.741903, whole genome shotgun sequence.
ACCESSION   DAORPD010000269 DAORPD010000000
VERSION     DAORPD010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9962_length_2062_cov_2.741903"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="PK976_03985"
     CDS             complement(<1..642)
                     /locus_tag="PK976_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67079.1"
                     /translation="MKTKIYFLILVLASIALLSMAASVLHTGSVQIEERGYSDYWRQI
                     DSLKELGQPATAARLTEELLSVALKENNSPQILKAHLYRIGLWAQYEEDYELKAIEAS
                     KKLINVTTTPAREILKSITAELYYLWYNNHQWELRGRGIMGDKLESDFRLWDARTFAD
                     TIASYYLASMDDPEKLYQIKAREFIPILDTAQGSEIFMPTLLDLLSHRALEFFS"
     gene            complement(829..1070)
                     /locus_tag="PK976_03990"
                     /pseudo
     CDS             complement(829..1070)
                     /locus_tag="PK976_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032488594.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          653 a          370 c          370 g          669 t
ORIGIN      
        1 gctaaaaaat tccaaggcac gatgcgataa taaatcgaga agggtaggca tgaaaatttc
       61 gctgccttgg gctgtatcca aaataggaat aaattctctg gctttgatct gataaagttt
      121 ttcagggtca tccatcgaag caaggtaata actggcaata gtatcagcaa aagttctggc
      181 atcccacaat cggaaatccg attccaattt atcacccatt attcctctcc ctcttaattc
      241 ccattgatgg ttattgtacc ataaataata aagttcagcc gtaatacttt ttaatatttc
      301 ccgagcaggc gtagtggtta cattgatgag ttttttactg gcctctatgg ctttcaactc
      361 ataatcttct tcatattgcg cccataagcc aatacgataa agatgtgctt tcagaatttg
      421 aggtgaattg ttttccttca atgccacaga tagcagttct tcggttaatc tggcggccgt
      481 tgcaggctgt cccagctctt ttaaactgtc gatttgcctc cagtaatcag aataacccct
      541 ttcttcgatt tgaacagagc ctgtatgaag tacggatgca gccatactca ataatgctat
      601 tgaagccaag acgagtatta aaaaatatat tttagttttc attccataaa tttcaataaa
      661 aattaagggg actactaaaa attcattttt tattttttgg ttgagttgaa aagttaaggc
      721 aaatttataa tcaattttta tcagttttga caaattatga caatcttttg aaaaatttta
      781 gtgggaaact ctctatttca attctatatc tatctgtttt tcaataattt atttatttaa
      841 gatattcagt cgcacaatct gctcatatcg aaacagatta tatatcaaat tggtcaaacc
      901 tataatcgcc ttagctcaaa attttcctat tgttttcatt acaatccatg catgctttgc
      961 tccataaacg caaatacatg cgcaactctc ccacgtattc ttgatttttc ctggttattc
     1021 gccttctgtt cttcggttaa tggcttattc ctgtatccct tctcgtgaat gtgattgatc
     1081 atttgatatt tgactattat ttgctcttgt gcttttccaa cataagctta atctccccat
     1141 tgtctatgcc ctatatcttt gtaacttaag atatctccaa gtacttgcga atcatttaaa
     1201 ggtgcatatt tataacgaaa aaaagacgcc ataacgatta acggcatctc aaccactatt
     1261 tgagtatgtt gttgagaatt aaaaagtgat gaattttgaa cagatgaaag atagttataa
     1321 aacgggattt gttctgattt cgatagatga atacatcagg aagcatttag agagtcagca
     1381 aactgagact gaaaaggatc ttaggaaacg attaaacgcg gcattgaacg attataagaa
     1441 gggcatataa tgtttttgtg gtaacgatat ttgggtaata gggtcggcta ttgttagaaa
     1501 tgcttgcttt acgtgtataa caggcgaaag ttgcccagac catgattatg aaatagatga
     1561 ggcgatcatc aagagaccat ctacaaaaag acgaaggcat attgacacca tggataaaag
     1621 ttaaattcat ggacttttta ataacgatgg atttgaaatt aaccctaaac ttattaaaaa
     1681 gccttcatta tgtttgattt gtaccaataa tgacaatccc aatgaagaaa tactatgtaa
     1741 tctgacccgc tacgaccaaa gggataggga tgatttcaca tgctttgtat atataaagag
     1801 tatgattgat tcaaaaaaac gcacgacggt acaacgcgca gtcataagct ttagtgacac
     1861 accaatgtta tcaatttctg caatgctccc cgatgcgagg atttgtaatc caaggcgaga
     1921 tgagttacag ttcttgaagc catgaatcac gaaacgcagg gattgcaaat ctcaaagaac
     1981 cgaaattgga tggttggtta taacatttcc agcgggctct tgatgctgga tttggcaata
     2041 tcggtaatat gcatgtagat tt
//
LOCUS       DAORPD010000270         2057 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_9999_length_2057_cov_1.783217, whole genome shotgun sequence.
ACCESSION   DAORPD010000270 DAORPD010000000
VERSION     DAORPD010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9999_length_2057_cov_1.783217"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2057
                     /locus_tag="PK976_03995"
     CDS             <1..>2057
                     /locus_tag="PK976_03995"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRC67080.1"
                     /translation="IRTNGEFKNSDVVKDIVVSKSSAGLVYLRDVAQVKDTLKHIYVD
                     ERIDGEVGVRLMVQKQSGANTVQIANLVRERMDELSKELPSDVKIVNIIDTSEFIQSS
                     INNLSETLLYAFIFVTLVVFIFLGRWRSTFIILLTIPVSLIAAFIFLYAIGGTLNIIS
                     LSALAIAIGMVVDDAIVVLENITKHIERGSNPMEAAVFATREVGLAVVASTLVIVAVF
                     FPLTLMTGLTGVVFRQLGWIVSITITISVLAALSLTPMLSSRILRSNLYYNYHPNKFL
                     NAVTRFWGRFDDWYAKSLSYALRHKKLILSAGFVLFVGSLFLLNFIGTEFIPEQDNGR
                     IGINIEIPQGVRIDESKKLARDFENVIRQKYPEIELISTSIGIDEEGSMASAIQKAGS
                     YIITMTIRLSSVEERSRSVFEIVDSLRNDLTKYPIIASSYVSVGGSRNQMAATAMGGG
                     GNNVVVNIFGYDINKTNYIADQISSYMKTQPGFRDVTISRDKDRLEIQVIPDRTKLAQ
                     LGMNTAMLSSAIRNRVLGFTATKYREEGNEYDVIVKYNDDNLVSIDDIRNITFRGPQG
                     NLVRVNDVAEVREVYTTPNIEHENKTRVVRVEASLSGTDLGKATNMINSRLKTLDIPP
                     EIFVEISGAAKDMADTFRDLGLLFILIMVLTYIVMASQFESMREPFIIMFSIPFAITG
                     VLI"
BASE COUNT          630 a          363 c          422 g          642 t
ORIGIN      
        1 aattagaacc aatggtgaat tcaaaaacag cgatgttgtg aaagatattg tggtttcgaa
       61 atcgagtgcg ggtttagtgt atttaagaga tgttgcccaa gttaaggata cacttaaaca
      121 tatttatgta gatgaacgca tcgatgggga agttggagta agattgatgg tacagaagca
      181 aagtggagca aataccgttc aaattgctaa tttggtgcgt gaaagaatgg atgaactcag
      241 caaggaactc cctagtgatg taaaaatagt aaatatcatc gatacatccg aattcattca
      301 gagttctatc aacaatcttt ccgaaactct tttatatgcc tttatttttg ttactttagt
      361 tgtatttatt ttcttgggca gatggcgttc aaccttcatc atcctactca ctattccagt
      421 ttcacttatc gcagcattta tcttccttta cgccatcggt ggaacactga atatcatttc
      481 actttctgca cttgccattg ccataggtat ggttgtggat gatgctattg tagtgttgga
      541 aaacattact aaacatatag agcgaggtag taatcctatg gaagcggcag tatttgcaac
      601 aagagaagta ggtttagccg ttgtagcaag taccttagtt attgtagcag tatttttccc
      661 attgacttta atgacaggcc ttactggagt cgtattcaga caattgggtt ggatagtcag
      721 cataactatc acaatttcag tattagccgc attatcgcta actcccatgc tttcttctcg
      781 tattttgaga agcaacctct attataacta ccatcctaat aaatttttaa atgctgtaac
      841 acgattttgg gggaggttcg acgattggta tgcaaaatct ctaagttatg cccttcgaca
      901 taaaaaactt atcctttctg ctggatttgt tctctttgtt ggaagcttat tcttactcaa
      961 ttttattgga accgaattta tacctgaaca agataatggc agaataggta tcaatattga
     1021 aattcctcag ggagttagaa ttgatgaatc taagaagcta gctcgggatt ttgagaatgt
     1081 tataagacaa aaataccccg aaatcgaatt aatttcaacc agtatcggca tagatgaaga
     1141 aggttctatg gccagtgcca ttcagaaggc tggttcttac attattacca tgaccattag
     1201 attaagtagt gtagaagaac gttcacgtag tgtttttgaa atagtcgata gtttgcgcaa
     1261 cgatttaacc aaatatccca tcatagcttc ttcttatgtt agtgttggtg gttcaagaaa
     1321 tcaaatggca gccacagcta tgggtggtgg aggtaataat gtagtggtaa atatttttgg
     1381 ctacgatatt aataaaacta actatatagc cgatcaaatc tcaagctata tgaaaacaca
     1441 gcccggtttc agagacgtta ccatttcccg cgataaggac cgcctcgaaa tccaggtaat
     1501 acccgatcga actaaactgg cccaattggg tatgaataca gccatgttat cgagcgctat
     1561 tagaaatagg gtgttaggat ttacggctac aaaatataga gaagaaggaa atgaatacga
     1621 tgttattgtt aaatataacg atgataacct ggtttcgatc gatgatatac gaaatatcac
     1681 ttttagagga ccccagggca atttggtcag agtaaacgac gtagccgaag ttagggaggt
     1741 ttatactaca ccaaatatcg aacatgaaaa taaaacccgt gtagttagag tcgaagcctc
     1801 gctcagtggc actgaccttg gaaaagctac caatatgatc aattctcgac tgaagactct
     1861 cgacatccct cctgagattt ttgtcgaaat aagtggtgcc gccaaagata tggccgatac
     1921 ttttagagat ttggggcttt tgttcattct tatcatggtg ctcacatata ttgtaatggc
     1981 ctctcagttt gagtcgatgc gcgaaccttt tattatcatg ttttcaatac catttgctat
     2041 tacaggtgta ttaatcg
//
LOCUS       DAORPD010000271         2054 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_10021_length_2054_cov_2.037019, whole genome shotgun sequence.
ACCESSION   DAORPD010000271 DAORPD010000000
VERSION     DAORPD010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10021_length_2054_cov_2.037019"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(9..437)
                     /locus_tag="PK976_04000"
     CDS             complement(9..437)
                     /locus_tag="PK976_04000"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HRC67081.1"
                     /translation="MMKKLYQVSRLLLGMTFIFSGFVKGQDPLGTMFKIEDYLIAYGW
                     DWALPFALALSILLCTAEFVIGAGLILNYQTKLIQWLILLFMVFFTCITFYDALYNAV
                     SDCGCFGDAIKLTNWQTFFKNIVLLALVAILFYFFVVVYC"
     gene            complement(460..1005)
                     /locus_tag="PK976_04005"
     CDS             complement(460..1005)
                     /locus_tag="PK976_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1599 domain-containing protein"
                     /protein_id="HRC67082.1"
                     /translation="MQNTLLQYDEAVAECRKVFEAKLKDYGPSWRVLRLPSITDQIYI
                     KARRIRTLQSSSEQRINEGIKPEFIGIYNYSLIALIQHELGPEPENASPSNIDYLNSY
                     NRWSQKAKQLMLDKNHDYDEAWRQMRLSSLTDIILMKIKRIKQIEDNSGLTLVSEGIE
                     SNYLDIMNYAIFSLIKISENK"
     gene            1041..1196
                     /locus_tag="PK976_04010"
     CDS             1041..1196
                     /locus_tag="PK976_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67083.1"
                     /translation="MNLGSKDTNSLTTRGLLIGEKGIMIPFPAVMGILNITQDSFYDG
                     GRYLTED"
     gene            1218..1919
                     /gene="folP"
                     /locus_tag="PK976_04015"
     CDS             1218..1919
                     /gene="folP"
                     /locus_tag="PK976_04015"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HRC67084.1"
                     /translation="MLQEGASIIDVGAASTRPGAQEIPEIFEIEKIRKVVHTLIQHFP
                     DIIISVDTYRSLVAQTALDEGASIINDISAGDFDASMIPLVASRKVPYIMMHILGTPA
                     TMQINPHYNDVVTDIIDYFKHRIEVCNRYGLDSLILDPGFGFGKTLKHNYTLLSHLSS
                     FIKLGYPVLVGVSRKAMIYKLLNVQPEEALFGTVVANALALLNGAHIIRVHDVKPTIQ
                     AIKILDEYKDSLTNP"
BASE COUNT          635 a          397 c          380 g          642 t
ORIGIN      
        1 cagctgaact aacaataaac taccacaaag aagtaaaaaa gtattgctac taaagcaagt
       61 aaaacgatat ttttaaaaaa ggtttgccaa ttggtgagtt taatggcatc gccaaaacat
      121 ccacaatcgg aaacagcatt ataaagggca tcataaaagg taatgcaggt aaaaaaaacc
      181 atgaagagca aaataagcca ttgtatgagt tttgtctgat aattgagaat taatccggca
      241 cctattacaa attctgccgt gcatagaaga atggaaagtg cgagtgcaaa aggcaaggcc
      301 caatcccagc cataagcaat caaataatcc tctatcttga acatagtgcc caatgggtct
      361 tggcctttaa caaatcccga aaagataaaa gtcattccta aaagcagtct gctgacttga
      421 tagagttttt tcatcataag tcaaaagaaa tttttattct tacttgtttt ctgatatctt
      481 gatcaatgag aaaatagcgt aattcataat atccaaataa ttcgattcaa ttccttccga
      541 taccagtgta agtccgctat tatcttcgat ttgcttgatg cgttttattt tcatcagaat
      601 gatgtcggtt aatgagctca ggcgcatttg ccgccaggct tcatcgtaat cgtgattttt
      661 atccagcatg agttgtttgg ctttttgcga ccacctgttg tatgaattga gataatctat
      721 attgctgggg ctggcattct ctggctctgg gccaagttcg tgctgaatca aggcaatgag
      781 tgaataattg tagatgccta taaattccgg cttaatccct tcgttgatac gttgttcgct
      841 tgatgattgt aaagtacgta ttcttcttgc cttgatatag atttgatcgg taatggaagg
      901 aagccgcaaa accctccatg aaggcccata atctttcaat tttgcctcga ataccttgcg
      961 gcattctgcc accgcttcat catattgcaa taaggtattt tgcatgattt tcttaatttc
     1021 gcactataaa tcgaatactg atgaatttgg gcagtaaaga tacaaattcc cttacaactc
     1081 ggggcttact cattggcgag aaaggtataa tgattccttt tcccgccgtt atgggcatat
     1141 taaacattac tcaggattca ttctatgatg gtggtagata tctaactgaa gattaatgga
     1201 taaatagaac tgcactcatg cttcaagaag gtgcttccat tatcgatgta ggtgccgctt
     1261 ctacacgccc tggagctcag gaaatacccg aaatttttga aatagaaaaa attagaaaag
     1321 tcgttcatac gctcatacaa catttccctg atattatcat ttcggttgat acttaccgct
     1381 cgctggttgc acaaaccgcc ctggatgaag gtgcttccat tattaacgat atttcggcag
     1441 gcgacttcga tgccagcatg attccacttg tagccagccg gaaagtccct tacatcatga
     1501 tgcatattct tggcactcct gccaccatgc aaataaatcc tcattacaat gatgtagtta
     1561 cggatattat cgactatttt aagcatagaa tcgaagtttg caaccgatat ggattggata
     1621 gtctaattct cgatcccggc ttcggtttcg gcaaaacctt gaagcataat tatacactat
     1681 tgtctcatct ttcctcattt attaaactgg gttatccggt tttagtgggc gtttcgcgta
     1741 aagcaatgat ttataaatta ttaaatgttc aacccgaaga agcccttttt gggactgttg
     1801 ttgccaatgc gcttgcttta ttgaatggag ctcatattat acgtgttcac gacgtaaaac
     1861 cgaccattca ggccataaaa attctcgatg aatacaagga ttccctgact aatccttaat
     1921 aatttatttg aagacagttt taactggttt taatgaatgc ttgaatattc tttacatgtt
     1981 tttagtaaac aaaataaccg aaattcgtat agcaaatacc gaattaattt tcaaatagag
     2041 taaaaaatga aatt
//
LOCUS       DAORPD010000272         2053 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_10023_length_2053_cov_2.386887, whole genome shotgun sequence.
ACCESSION   DAORPD010000272 DAORPD010000000
VERSION     DAORPD010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10023_length_2053_cov_2.386887"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            267..863
                     /locus_tag="PK976_04020"
     CDS             267..863
                     /locus_tag="PK976_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010537505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HRC67085.1"
                     /translation="MLKSQQTIQLTPPDLIRGLPAMKTLSLRASATEFDTTMLSLKDI
                     SDLLWAANGINRPTSGKRTAPSAMNAQDIDIYSFMKSGVYLYDAGKHALQLVVTGDHR
                     RLFQRDEKSPAPPVILLLVSDVSRFRAGEDSLKMIWGAMDAGIVSENISMFCASENLA
                     TRPRASMNQKKLRELLNLKDSQYPMLNHPVSYKKNRTN"
     gene            complement(1215..>2053)
                     /locus_tag="PK976_04025"
     CDS             complement(1215..>2053)
                     /locus_tag="PK976_04025"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC67086.1"
                     /translation="SGIDIGKMVIDDYDQKWVLMRKEHSLLVFNETLTQGKPYKILGS
                     ATGEGAIPGNMVLSIAVDLDGEIWIGTDAGVAVFYNPDAVFSGYNFDAQRILVEQDGY
                     VQYLLENESVTAIAIDGANRKWIGTDKAGVFLLSTDGTQQIYHFTEDNSPLLSNSVAD
                     IAIGKDGEVFIGTSLGIVGFRGDSPSPGPPSDEVYAFPNPVRPSYDGPIGISGLPQNA
                     DVKITTISGKLVYHTVAAGTQAVWNGRDFDGRRVQSGVYLVFVSNEDGSEKLITKILF
                     IH"
BASE COUNT          602 a          437 c          410 g          604 t
ORIGIN      
        1 catggcggtt tatcgctcga ggaaattctt gtgcctttcg tatatctcaa ggcaaaataa
       61 agagggtaag aaatgattca aaagagtttt tctattcgct tcgaattatt tttaagacgg
      121 caacataatt tttatgtata tttacgaaaa ctttcaaggt gactacctta tttcttaaat
      181 tctaaacgtt tatctcaaac cttttgaata tgaataagat tgctcattat gtttcatttt
      241 cagttttgtt tttactgacc tcaaccatgt taaaaagtca acaaactatt cagctcacgc
      301 ctcccgatct tatcaggggt cttcccgcca tgaaaacctt atcgcttagg gcttctgcaa
      361 ccgaattcga tacgaccatg ctttcgttga aggatatatc cgatctattg tgggctgcca
      421 atggcataaa ccggccaacg tcgggcaaac gcactgcccc ttcggctatg aatgcccagg
      481 atatcgacat ttattccttt atgaaatcgg gagtatatct gtacgatgca gggaaacatg
      541 cacttcagct tgtggtaaca ggcgatcaca gacgtttgtt ccaacgcgac gaaaagtcac
      601 ctgctccacc tgtaattctg ctacttgttt ccgatgtctc tcggttcagg gctggagaag
      661 attcattaaa aatgatttgg ggcgcaatgg acgcaggtat tgtttcagag aatatttcga
      721 tgttttgtgc ctcagaaaac ctggctaccc gtcctcgagc cagtatgaat cagaaaaaac
      781 taagagaact gctcaacttg aaagattctc aataccctat gctgaatcat cctgtatctt
      841 ataaaaagaa tcggacgaat taattcagaa tatcgccact aaactctccc tgattcatag
      901 cttttcgaac aaaatttctg tgtaaaggcc gttcctacat aaaatttgat tatttagggc
      961 gaattggagg atggagagta gaatgtggaa cgaaatgaaa atgacaaata aaggagttga
     1021 gtgaaaagcg aaaaagcatt tcacggtgag tgatttatga agcagcttag gtcttttcgt
     1081 attacctatt cactgatcat ttgaaatacc aaaggataat tatcatattc gccttctcgc
     1141 ccattctacc ttcttgcagt aattttattt tccagtgaat aaatcttatt cgttttaagg
     1201 tctgcaaaac attgctaatg aatgaataaa atcttggtga tcagtttttc gcttccatct
     1261 tcgttagaaa caaaaaccag ataaactccg ctttgcactc ttcggccatc gaaatcgcgg
     1321 ccattccata cggcttgtgt tccggcggct acggtatgat aaactagctt tccgctgata
     1381 gtagtaattt ttacatcggc attctgcgga agtcctgaaa ttccaatagg tccatcataa
     1441 gatggacgta cgggatttgg gaaagcataa acctcatcag aaggaggccc aggtgaaggg
     1501 ctgtcgcctc taaagccaac gattcccaaa gaagttccaa taaatacttc cccatctttg
     1561 cctatggcga tgtcggctac cgaattggaa agcaatggac tgttatcttc ggtaaaatga
     1621 taaatctgtt gggtgccatc ggtcgaaagc aagaaaacgc ctgctttatc ggttcctatc
     1681 catttgcgat tggctccatc tatggcaatt gcggtaaccg attcattctc gagtaaatat
     1741 tgaacatagc catcctgttc gaccaatatg cgctgggcat cgaaattata gcccgaaaat
     1801 acggcatcgg gattgtaaaa aacagctaca ccagcatccg taccaatcca gatttctcca
     1861 tcgaggtcaa cagcaatgga aagaaccata tttccaggaa tagcaccttc gcctgtggcg
     1921 gaacccaaaa ttttataagg tttaccctga gtaagggttt cattgaatac gagtagagag
     1981 tgttctttgc gcatcaatac ccatttttgg tcgtaatcat cgatgaccat tttaccgata
     2041 tcgataccgc tgg
//
LOCUS       DAORPD010000273         2049 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_10051_length_2049_cov_2.589769, whole genome shotgun sequence.
ACCESSION   DAORPD010000273 DAORPD010000000
VERSION     DAORPD010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10051_length_2049_cov_2.589769"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="PK976_04030"
     CDS             complement(<1..655)
                     /locus_tag="PK976_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006255681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HRC67087.1"
                     /translation="MTEPGRYQQRGVSSNKEDVHAAIAAIDKGIEPKAFCKIIPDVLG
                     GNPEYCNIMHADGAGTKSSLAYIYWKETGDLTVWKGVAQDAIVMNTDDLLCVGLFDNL
                     LISSTIGRNAHFIPGEVIHAIIQGTEEVLQWLRRMGITIYSTGGETADVGDLVRTIIV
                     DSTIIARARRSDIITNANIRPGDVIVGLASFGQANYESSYNSGIGSNGLTSARHDVLH
                     "
     gene            complement(660..>2049)
                     /locus_tag="PK976_04035"
     CDS             complement(660..>2049)
                     /locus_tag="PK976_04035"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRC67088.1"
                     /translation="PGLGGLDIFKAYFRTDKEGRKYFTRPIHMGVPLNSNFDDFGIVF
                     HPEAEEEGFFASNRQGGRGSDDIYSFVVEPVVFTLEGLVKDESTLQPIAGALVEMKVS
                     DGSQHKSNTSERGRYFFNQNQVKPNRNFDLSVSKEGYFTKTGSISTIGLESSKDLVLD
                     FVLQPIPRKPIVLPEILYDLGKWDLKPQYQDSLQGLITILEKNPNLEIELAAHTDARD
                     TEEHNDILSQKRAQSVVDYLVQRGIDPDRLTAKGYGERVPRVLTRDIKKAGYTFKAGT
                     RLTESFIDSLPNREVKEAAHQLNRRTEFSITGTNFKSKPKPQGQQFAPKIEIVTTPPK
                     NSVTYILSAKKNRMLIPASIDTYSLEFLYEPNTKYVSISMDAALDLLKQNIITKDDFQ
                     GNPEEIISEGQIKRGSVLTLPSLRIGRKMIYDVDVIVEPKQDEKLILNNEILSRFGAF
                     TINEDTREIIFE"
BASE COUNT          491 a          445 c          479 g          634 t
ORIGIN      
        1 tatgcagaac atcatgcctg gccgaggtca gtccattgct gccaatgcca ctgttgtatg
       61 aagattcgta attagcctgt ccaaaggaag ccaaacctac tatgacatcg cccgggcgga
      121 tatttgcatt ggtgatgatg tctgatcgtc ttgcgcgggc gataattgtc gaatcaacga
      181 taattgtgcg taccagatct cccacatcgg cagtttctcc tccggtcgaa tagatcgtaa
      241 ttcccatcct cctcagccat tgcaacacct cttctgttcc ttgtatgatc gcatgaatta
      301 cttcaccggg aataaaatgc gcattgcggc caatcgtgga cgaaatgagg agattatcga
      361 ataatcctac acacaacaaa tcatccgtat tcatcacaat agcatcttga gccacacctt
      421 tccaaactgt cagatcgcca gtttctttcc agtaaatgta ggctaggctg cttttagttc
      481 cggctccgtc ggcatgcatg atgttgcaat attcggggtt gcctcccaaa acatcaggaa
      541 ttatcttgca aaaggcttta ggctctatgc ctttgtctat ggcagctatg gctgcatgca
      601 catcttcttt gttcgaagat acgcctcttt gttgataccg gccgggttct gtcataaatt
      661 tattcgaaaa taatttctcg ggtatcttca ttgatggtga aagctccaaa acgagataat
      721 atttcattat tcagaatgag tttttcatct tgttttggtt cgacaatcac atcaacatca
      781 taaatcatct ttctgccgat tcgcagggag ggtagggtca gcactgagcc acgcttgatc
      841 tgtccttccg aaataatttc ttcgggattg ccctgaaaat cgtctttggt gatgatgttt
      901 tgtttcaaaa gatctaaggc cgcatccatc gaaatgctta cgtattttgt attgggttcg
      961 tataggaatt cgagcgaata ggtatcgatt gaagcgggaa taagcatacg gtttttcttg
     1021 gccgagagga tgtaagttac gctatttttt ggtggggtgg ttacgatttc gattttaggt
     1081 gcaaactgtt gaccttgagg ttttggttta gatttaaaat tcgttccggt gatagaaaat
     1141 tcggttcttc tgttgagctg gtgggcagct tccttcactt cacggttggg taagctgtcg
     1201 ataaacgatt cggtaagtct tgttcctgcc ttgaaagtgt atcctgcttt tttaatgtcc
     1261 cttgtaagca cgcgaggaac acgttctccg tatccctttg ccgttagtct atcgggatcg
     1321 atccctcgtt gaaccagata atcgactacc gactgggctc gtttttgcga caagatatcg
     1381 ttatgttctt cggtgtctcg ggcatcggta tgggctgcca gttcaatttc gagattggga
     1441 tttttttcga gaatagtgat gagcccttga agagaatcct gatattgagg tttcaagtcc
     1501 catttaccca aatcgtagag gatttcggga agaacgatcg gttttcgagg tataggctga
     1561 agaacgaagt cgagtactag gtctttactg ctttcgagac ctatggtaga aatgctgcct
     1621 gttttggtaa aatatccttc tttgcttacg cttagatcga aattacggtt gggtttcacc
     1681 tggttctgat tgaagaaata gcggccacgt tcgctggtat tactcttgtg ctggctgccg
     1741 tcggatactt tcatttcgac cagtgctccg gctatgggtt gaagtgtact ttcatctttt
     1801 acgaggcctt cgagggtaaa aacaacaggc tcgacaacga aactgtagat atcatcacta
     1861 cccctaccac cctgtcgatt cgatgcaaag aacccttctt cttcggcttc ggggtgaaac
     1921 acgataccga agtcgtcgaa gtttgaattc agaggaacgc ccatgtggat aggcctggtg
     1981 aaatatttgc ggccttcttt atcggtgcgg aagtaagcct tgaaaatatc gagccctcct
     2041 aagccggga
//
LOCUS       DAORPD010000274         2048 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_10060_length_2048_cov_2.302559, whole genome shotgun sequence.
ACCESSION   DAORPD010000274 DAORPD010000000
VERSION     DAORPD010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10060_length_2048_cov_2.302559"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1251)
                     /locus_tag="PK976_04040"
     CDS             complement(<1..1251)
                     /locus_tag="PK976_04040"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HRC67089.1"
                     /translation="MKKLQFFILTVSIFVGLLPYRSIAQGWRSNEMEINVLVKTLEDM
                     ELIHRSGWSYDRPSSIPGIARLYLIPKELEELKQMGMEYSISIPDIAEHYRDLWGGVV
                     PPGYYTYEQLIAIEDSLAAAFPAICKKIVIGTSVQNRQLAVLKISDNMEQDEAEPEIF
                     FEAGIHGDEVGGPENLIRFARDLCKGYGSNPQITNLVNTRELFILVMTNPDGRVQMSR
                     YNANLVDINRDGGYMWDGEGNSPGAFSQTETKAIRDFAAQNQFVVFTDYHSGTEFISY
                     PWSYRPQMAPDQPHLHQLAHVYATNSGYTNIPYASGYSGMYPINGSTKDFNYGLQGSI
                     SWSIEISNNKQPPASQIYYYYLKNKGAMIAISEQCGKGIYGVVTDSVTGNPIPAKIRI
                     GGTVPLYNDPVVGDYHKFVVPGIYT"
     gene            complement(1476..>2048)
                     /locus_tag="PK976_04045"
     CDS             complement(1476..>2048)
                     /locus_tag="PK976_04045"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC67090.1"
                     /translation="EHAYAQRYNQRDSLIVLASDDCGSTWTRIFASGPDGNGIFETAP
                     KTESEFIPSTAEDWCGSGWGANCIQLDLSAWAGKPNCRIAFEAFNKLGNNLYIDNIVV
                     SRSGVGMAHSVENTFIVSPNPSNGIFNITTPYHNQQRAEIYSTDGRMLFSKDFSNNNL
                     KINVSSYPSGIYTLKIIANQQTYQIKLIKK"
BASE COUNT          564 a          420 c          438 g          626 t
ORIGIN      
        1 agtgtaaatc cccggtacca caaatttgtg ataatctccg accacaggat cattataaag
       61 aggaacagtc cctccaatac gaatttttgc cgggattgga tttcccgtaa ccgagtctgt
      121 tacgacacca tagattcctt tgccacattg ctctgaaatt gcaatcatgg ctcctttgtt
      181 ctttagataa taataatata tttgagaagc aggcggttgc ttgttatttg agatttcgat
      241 cgaccaggaa atgcttccct gcaatccata gttaaaatct ttagtgctcc cattgatagg
      301 atacatacca ctgtagccag aggcataggg aatattcgtg tagccggaat tggtagcata
      361 aacatgagca agttggtgta aatgaggttg atcgggtgcc atctgaggcc tgtaactcca
      421 tggataactt ataaactctg tcccgctgtg gtaatcggta aaaacgacaa actggttttg
      481 tgcggcaaag tctctaatgg cttttgtttc cgtctgagaa aaagcaccag ggctattgcc
      541 ctcaccatcc cacatataac ctccatctcg gttgatatct accagattgg cattgtaacg
      601 gctcatctga acccgtccgt cgggattggt cattactaaa atgaataatt cacgtgtatt
      661 taccagatta gtaatttgtg gattcgaacc ataccccttg cataagtctc gggcaaacct
      721 gatgaggttt tcggggcctc ccacttcatc cccatgtatg cctgcttcga aaaatatttc
      781 gggctcggct tcgtcttgtt ccatattatc actgattttc aaaactgcca gttgacgatt
      841 ctgaacgctt gtacctatga cgattttttt gcaaatagct ggaaatgctg cagcaagact
      901 atcctcgatt gcaatcaatt gttcgtaagt gtaatatcct ggcggtacaa cccctcccca
      961 aaggtctcga taatgttccg caatatcggg aatacttatg gaatattcca tgcccatttg
     1021 ttttaattct tcgagttctt tgggaatgag ataaagtctg gctatgcccg gaattgatga
     1081 aggtcgatcg taactccatc ctgaacgatg aatgagttcc atatcttcca gagtttttac
     1141 caaaacattt atttccattt cattactacg ccagccttgt gctatacttc gatagggcaa
     1201 caagccaacg aatatagata ctgtcaaaat gaaaaactgc aattttttca tttgttttcg
     1261 gttttgttta aagagataaa aatttgaacc acattatttt gaagtgataa tttcgaggac
     1321 gcatgaagaa aatgagccaa agattatcat tcgaaagagc aacgataggg tatgacagaa
     1381 tatattcgac gagtgaggtt cgaataggat ttgagcgata acggaagtgg tctgcaagat
     1441 ttgtagcacc tgcagaccat ctcagttatg attctctatt tcttgattaa tttgatctga
     1501 taggtttgtt gattggctat aatttttaat gtatagatgc ctgagggata agaagaaaca
     1561 ttgattttca ggttgttgtt gctgaaatct tttgaaaaca gcatacgtcc atcggtggaa
     1621 tagatttctg ctctttgttg gttatgatag ggggttgtaa tgttgaagat tccattcgaa
     1681 gggttggggc taacgataaa agtattttca acggaatgag ccattcccac acctgagcgg
     1741 cttacaacaa tattgtctat ataaagatta ttgcccaatt tattgaatgc ctcgaaagcg
     1801 atgcgacagt tgggtttgcc ggcccaagca cttaggtcga gctggatgca atttgctccc
     1861 cagcccgagc cgcaccagtc ctcggcggta gatggaatga attctgattc ggtctttggg
     1921 gcagtttcga agatgccatt gccatcgggc cccgaagcaa atattcttgt ccatgtactt
     1981 ccgcagtcat cactcgccag cactattaaa gaatctcgtt gattatagcg ctgtgcataa
     2041 gcatgttc
//
LOCUS       DAORPD010000275         2036 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_10159_length_2036_cov_2.156487, whole genome shotgun sequence.
ACCESSION   DAORPD010000275 DAORPD010000000
VERSION     DAORPD010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10159_length_2036_cov_2.156487"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..811
                     /locus_tag="PK976_04050"
     CDS             89..811
                     /locus_tag="PK976_04050"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HRC67091.1"
                     /translation="MKKFIGLLLVMVAALNIFGQQSEKKYQFGIKVSPNYTWINPETR
                     NYKNDGGKIGFTWGLIADINLRDNYFFSSGFGLSYQGGKICYPVNYPIPKPKTNGIGD
                     TTLILGKVYRNYSYRYLEIPLLFKMKTLPKKNTRFYGDMGLVPAFKLRAHANDIFYSQ
                     QGTTLKNEPDVSSALNAMKLSIFVGGGFEYHFDQSTFIMIGLGFNNSFTDIFSDYNSH
                     PSVNASEDGIANYLELSLALFF"
     gene            837..>2036
                     /gene="nadE"
                     /locus_tag="PK976_04055"
     CDS             837..>2036
                     /gene="nadE"
                     /locus_tag="PK976_04055"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HRC67092.1"
                     /translation="MKIALIQLNYIIGDFEGNTKKIIQSIQEAKSKGASLAIFSELSV
                     CGYPPKDLLEFDDFIEKCEESVQKISRACRDIAAIVGSPIKNETRKGKPLFNAALLLY
                     EGQVLGIQRKTLLPDDDIFDEYRYFEPNQQFDVFEFRGERIALCICEDLLDTDNNPLY
                     LCKPQDELLKLNPSLMVNISASPFHDNQTELRPKILKENTVRSQIPLIYVNQVGAHTD
                     LIFDGDSMVIDIYGDIVARMKLFAEDMVIVDTKKLHSISNALPHYPLSRIEMIHDALV
                     FGIQEYFGKLNLKNAILGLSGGIDSALVLVLAKEALGNDHVKALILPSHYTAPQSITD
                     AILLSQRLEVDFEIISIDKAYKSLENTFEYFFKKMPSDVTEENLQARLRGIILMAFSN
                     KFGHILLN"
BASE COUNT          656 a          388 c          372 g          620 t
ORIGIN      
        1 cctacattac agggaacaaa acaatacttt tatttcaacc tttataaatc aataaatgcc
       61 tgtatctttg tttaaaattc aaactaccat gaaaaagttt atcgggttat tgcttgtgat
      121 ggttgcggct ctgaatattt ttggccaaca aagcgaaaag aagtatcaat ttggaataaa
      181 agtcagtcca aattacacct ggataaatcc tgaaacgcga aactataaaa acgatggtgg
      241 aaaaattgga ttcacgtggg gtttaattgc tgacatcaat cttcgagata attatttctt
      301 ttccagcggg tttggattaa gttatcaagg aggtaaaatt tgctacccgg taaactatcc
      361 aatacccaaa cctaagacta atggaatagg tgatactaca ttgattctcg gaaaggtcta
      421 ccgcaattat agctatcgtt atcttgaaat tcctttacta ttcaagatga aaacactacc
      481 caaaaaaaac actcgatttt atggagatat gggtttagtg cctgctttta aactaagagc
      541 ccatgctaac gatatttttt attcccaaca gggaacgacc ttgaaaaacg aacccgatgt
      601 ttcatcggct ctaaatgcta tgaaactctc catttttgtt ggaggtggat tcgaatacca
      661 cttcgatcaa tccaccttta tcatgattgg attgggtttc aataattcat ttacggatat
      721 cttttccgat tataactccc atccttcagt aaatgcttcg gaagatggga ttgccaatta
      781 tttggaactt tctttggctt tatttttcta ataatcaaag cgttagatcg gatcttatga
      841 agatagctct gattcaactc aattacatca ttggtgactt cgaaggaaac acaaaaaaaa
      901 tcatccaatc tatacaggaa gcaaaatcaa aaggagcttc gctggctatt ttttccgaac
      961 tctcagtatg tgggtatccc ccaaaagatt tattagaatt cgatgatttt atagaaaaat
     1021 gcgaagaatc ggttcagaaa attagtcgag cctgtcgaga tattgctgcc attgtgggaa
     1081 gtcccataaa aaacgaaaca aggaaaggga aacccttgtt taatgctgca cttttattat
     1141 atgaaggaca agttcttggc attcaacgca aaaccttgct ccctgatgat gatatattcg
     1201 atgaatatcg ctactttgaa cccaaccagc agttcgatgt ttttgaattt agaggtgaaa
     1261 gaattgcgct ttgcatctgc gaggatttac ttgataccga caataaccca ctttatttat
     1321 gcaaaccaca ggatgaacta cttaaattaa atccatcgct tatggtgaat atttcggctt
     1381 caccatttca cgacaatcaa acggagctga ggccgaaaat actgaaagaa aacaccgtaa
     1441 gatcccaaat acctcttatt tacgtcaatc aggtgggcgc acataccgat ttgatattcg
     1501 acggagattc gatggttatt gacatttatg gagatatcgt agcacgcatg aaactttttg
     1561 ccgaagacat ggtgattgtc gataccaaaa aactccattc gatatccaat gccctgcctc
     1621 attatcccct ttcacgtata gaaatgatac atgatgcact ggtttttggc atacaggaat
     1681 attttggcaa attaaacctg aaaaatgcca ttctaggact ttcgggcgga atagattcgg
     1741 cattggtgct ggttttagca aaggaagcac ttgggaatga tcatgtaaag gctctcattc
     1801 taccctctca ctataccgca cctcaaagta ttactgacgc tattctatta tctcaacgat
     1861 tagaggttga ttttgaaata ataagcatcg ataaggcgta taaaagttta gaaaacacat
     1921 ttgaatattt tttcaagaaa atgccttccg atgtcaccga agaaaatctt caggcgcgcc
     1981 ttagaggaat tatcctgatg gctttttcga ataaatttgg ccatattttg ctcaat
//
LOCUS       DAORPD010000276         2035 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_10165_length_2035_cov_1.332323, whole genome shotgun sequence.
ACCESSION   DAORPD010000276 DAORPD010000000
VERSION     DAORPD010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10165_length_2035_cov_1.332323"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="PK976_04060"
     CDS             complement(<1..324)
                     /locus_tag="PK976_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008154288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HRC67093.1"
                     /translation="MAKNLVIVESPAKAKTIESFLGKDYMVKSSFGHIRDLDKNKLSV
                     DIQHDFTPQYEIPREKKKVIEELLAAARKAEIIWLASDEDREGEAIAWHLYEVLGLEE
                     SHTRRI"
     gene            473..1813
                     /gene="miaB"
                     /locus_tag="PK976_04065"
     CDS             473..1813
                     /gene="miaB"
                     /locus_tag="PK976_04065"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HRC67094.1"
                     /translation="MSENRLYIETYGCQMNFSDSEIVASIMTSEGYSLVNDPHQANLI
                     LINTCSIRGKAEERVWKRLRELKSAKKLNPELKIGVIGCMAERLADKLLSDEQVVDLV
                     AGPDAYRFLPKMVAESFHGAQAANIQLSLEETYADIEPVRLDPSGVNAFISIMRGCEN
                     FCAYCVVPFTRGKERSRHPQTILHEAENLFQKGYREITLLGQNVNSYHFKDDRLDMDF
                     SDLLIEVAKINPSLRVRFTTSHPKDLSNKLISAISSYPNICKSIHLAVQSGSDVILKR
                     MNRKYTRSWFLDRILTIRKMIPEVTISTDIITGFCGETEEDHRQTLSLMEEVRFDSAF
                     MFRYSERPGTIAAKKFSDDVPSEIKAKRLQEIIELQHKHSLYSNRQDVGKTFEVLVEG
                     FSKRSRSMLSGRNSQNKMIVFKPKKSSIGQYTQVKITGCTQATLIGEELENTTQ"
BASE COUNT          629 a          404 c          374 g          628 t
ORIGIN      
        1 aatgcgtctt gtatgtgact cttccagtcc taaaacttca taaagatgcc aagcaatggc
       61 ttctccttcc ctatcttcat cggaggccag ccaaataatc tcagccttac gcgcagctgc
      121 taaaagttct tcaatgacct ttttcttttc cctcggtatt tcatattgag gagtaaaatc
      181 atgctgaata tctacactta acttattctt atcgagatca cgaatatgac caaaactcga
      241 ttttaccata taatccttcc ccaaaaaact ctctatggtt tttgccttgg ctggagattc
      301 tacaataacc agattttttg ccatgctaaa aaatattaat gctttagttt gaaatgagtg
      361 caaaagtaat attttgaaat tattgctcta tttcgtcata aacgactgag ataagccatt
      421 ttcagccctt ggtaatatta tggctaattt tgcatccgat taatatagtg tgatgtcaga
      481 aaatcgtttg tatatagaaa cctatggctg tcagatgaat ttctctgaca gtgagatagt
      541 ggcttccatc atgacttcag aaggttattc attggtgaat gaccctcatc aggctaatct
      601 tatacttatt aatacttgct cgattagggg gaaagctgaa gaaagggttt ggaaaagact
      661 tagagagctc aaaagtgcga agaaattgaa tccagagctt aaaattggcg taattggctg
      721 tatggccgaa cgtttggctg acaagttgtt gtccgatgaa caggtagtag atctggtagc
      781 cgggcctgat gcctatcgtt tcttgcctaa gatggtggca gaatcgtttc atggcgcaca
      841 ggcagccaat attcaacttt cactcgaaga aacttatgca gacattgaac ccgttcggct
      901 cgacccttct ggtgtaaatg catttatttc catcatgcga ggatgcgaaa atttttgtgc
      961 ctattgtgtg gttccattta cccggggaaa agaacgtagc cgccacccac aaactatcct
     1021 ccatgaagca gagaatcttt tccaaaaagg atatagagag attacacttt tagggcaaaa
     1081 tgtcaattca tatcatttta aagatgacag actcgatatg gattttagcg atttgcttat
     1141 agaagtggca aaaattaacc cgtcattaag agtcagattt actacttcac atcctaaaga
     1201 tctttccaat aaacttattt cggccatctc gtcctatccc aatatctgta aatcgattca
     1261 tctggcggtt caatcgggga gtgatgtcat actgaaaagg atgaaccgta aatacactcg
     1321 cagctggttt ctcgatcgta tccttacaat tcgtaaaatg attcctgaag tcactatttc
     1381 aaccgatatc attactggat tttgtggaga aaccgaagaa gatcaccgac aaactctttc
     1441 attgatggaa gaagtgcgtt tcgattcggc ttttatgttt cgttattccg agcgacctgg
     1501 aactattgct gcgaaaaaat tctccgacga tgttccttct gaaattaaag ctaaacgttt
     1561 gcaagagatt atcgaacttc agcataaaca ttcgctctat agtaatcgac aggatgttgg
     1621 gaaaaccttc gaggtattag tagaagggtt ttcgaaaaga agtcgctcaa tgctttcagg
     1681 acggaattca caaaataaaa tgatagtatt taagcctaaa aagtcaagca tagggcaata
     1741 tacacaggtt aaaataacag gctgtaccca ggcaaccctc ataggcgaag aattagaaaa
     1801 tacaactcaa taaatccttt tccataccta ctgatcctat ggatattaga aaagatctta
     1861 tcacgagcca tattctattt ctctaacctt tataaagaaa accatttgaa tttttctaag
     1921 attttacaat caaggcaatt ccattgaaac gatcgagctg gttaccatca ataaaaacta
     1981 cttctccttt ctgatttatc attttttcta tcatttcgtc gatgatatct tcaac
//
LOCUS       DAORPD010000277         2032 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_10186_length_2032_cov_2.589277, whole genome shotgun sequence.
ACCESSION   DAORPD010000277 DAORPD010000000
VERSION     DAORPD010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10186_length_2032_cov_2.589277"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2032
                     /locus_tag="PK976_04070"
     CDS             <1..>2032
                     /locus_tag="PK976_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67095.1"
                     /translation="AGNETSVPLMEWPSNSRTILDGIEYSGQHNIIGAGVYIGANKEG
                     LPGKSNRLFALCGAVGASDPEVAIGRWSFPLYMEEIENFPLNSDGSLNENYNPNEAEE
                     IISAGWSTPTGISVYRTSRAWSYPDYDDLIIYEYEFEYTGDTDGDPSTIEMNDKLKDV
                     MICFNYGLYPSMYGAQRVYLEWKYEGGLYRGDVNNFWDADYWLTWNMNLRNNITDENG
                     MGKPEPNPDYFLQFARSGEHGGGLNSPQAPGYCVLFYDTTHLAVVIPPEMDTLGLNES
                     EAQAILRSTTVTSLSQYSGIDVYTETTGGYKWYYELDKSFHIRQPWDNKVSTGNTNSE
                     KIMYEKEPFNPSTRWSGVYSPGSATWPEVKSDDWYGRAAYPYRQSADAGMRLHAFGPY
                     TLKPGDKIRLAYAEVIGYGAQEGKRVEGGQTATQWAKIPSLNRKVTYKDSKGNEKVLT
                     EHYLDDFGYPDYVNSNIITVHDVAQKAFEAYLGNEPVVPVQPEKHPSRGTYSVPTPPP
                     APVISIKNYGLAEVKITWGRAQEQFTHPRLTGDLAKYMVQRAVSGMGPWITLDTIDVG
                     DNEYLNENDEYEYIDSDPTYNVGEFRYYAVVSVDEHGNKSGKTNMTCFQKNIPAVEKI
                     DKVYVVPNPFICKSGFTTEGDERMIGFYGLPDVCTIRVFSYSGQLIDTIEHKN"
BASE COUNT          608 a          369 c          471 g          584 t
ORIGIN      
        1 gctggtaatg aaaccagtgt ccccttaatg gagtggccat ctaactccag aacgatttta
       61 gatggaattg aatatagcgg acaacataac attattggcg ccggtgttta tatcggtgcc
      121 aacaaagagg ggttaccagg taagtccaat cgtttatttg ccttatgtgg tgccgtaggg
      181 gccagtgatc cggaagttgc aattgggcgc tggagctttc ccttatatat ggaagagatt
      241 gaaaacttcc cgttaaattc agatggttcg ctgaatgaaa attataatcc aaatgaagct
      301 gaagaaatta tcagcgctgg ttggtccaca ccaacgggaa taagtgttta ccgtacatct
      361 cgggcttgga gctatcccga ttacgatgat ttaatcatct atgaatacga atttgaatat
      421 actggcgata cagatggtga cccctccacg attgaaatga atgataaatt aaaagatgta
      481 atgatttgct ttaattatgg tttatacccg tcgatgtacg gagctcaaag agtttatctt
      541 gaatggaaat atgaaggcgg tctatatcgg ggagatgtta ataatttttg ggatgctgat
      601 tattggctga cctggaatat gaatcttcgc aataatatta ctgatgaaaa tggcatgggg
      661 aagcccgaac caaatcctga ttattttctc caatttgccc gaagtggtga acatggtgga
      721 ggactaaatt caccccaagc tccaggctat tgtgttcttt tttacgacac tactcacctg
      781 gcagtcgtaa ttcccccaga aatggataca ttgggtttaa atgaatcgga agcccaagcg
      841 attcttcgtt cgactacagt tacatctctt agccaatatt ctggaattga tgtatataca
      901 gagacaacag gtgggtataa atggtactat gagttagata aaagttttca tattcgacag
      961 ccatgggata ataaagtcag tacgggcaac accaacagtg aaaaaataat gtatgaaaag
     1021 gagccgttta atccatcaac acgctggtct ggggtttata gccctggcag tgctacctgg
     1081 ccggaagtaa aaagtgatga ttggtatggt cgagcggcct atccttatcg tcaaagtgct
     1141 gatgctggaa tgcgcttgca tgccttcggt ccatatacct taaaacctgg tgataaaatc
     1201 cggctggctt atgccgaagt aattgggtat ggtgctcaag agggtaaaag agttgaagga
     1261 ggtcagaccg caacacaatg ggcaaaaata ccgtcgctaa atcggaaagt tacctacaag
     1321 gatagtaaag gtaatgagaa ggttttaact gagcattact tggatgattt tggttatcct
     1381 gattatgtta attctaatat aattactgtt catgatgtag cccagaaagc tttcgaagcc
     1441 tatctcggga atgaacccgt ggtacccgtt cagccagaga aacatccttc acgaggaaca
     1501 tattcagtac ctacaccacc acctgccccg gttatttcaa taaagaatta cggtttggct
     1561 gaagtaaaaa taacttgggg cagagctcag gaacaattta cccacccacg tttaactggt
     1621 gatttggcaa agtatatggt tcaacgcgct gtctccggga tgggcccgtg gattacactt
     1681 gatactattg atgttggcga taatgaatat ctcaatgaga atgatgaata tgagtacatt
     1741 gattccgacc cgacctacaa tgttggtgaa tttcgttact atgctgttgt atctgttgat
     1801 gagcatggta ataaaagcgg taaaactaat atgacctgtt ttcagaaaaa tattccggcg
     1861 gttgaaaaaa tagataaagt ctatgtcgtt ccaaatccgt ttatttgcaa atcaggcttt
     1921 accactgaag gtgacgagag gatgatcggt ttttatggat tgcctgatgt ttgtactatt
     1981 cgagtgtttt catattcggg tcagttgatt gatacaatcg agcataaaaa tt
//
LOCUS       DAORPD010000278         2028 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_10217_length_2028_cov_2.597567, whole genome shotgun sequence.
ACCESSION   DAORPD010000278 DAORPD010000000
VERSION     DAORPD010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10217_length_2028_cov_2.597567"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..172)
                     /locus_tag="PK976_04075"
     CDS             complement(<1..172)
                     /locus_tag="PK976_04075"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRC67096.1"
                     /translation="MSYTYEYPRPCVTVDVALFRKHHDKEEILLIQRKNPPFEGMWAL
                     PGGFINMDENLSE"
     gene            complement(185..985)
                     /locus_tag="PK976_04080"
     CDS             complement(185..985)
                     /locus_tag="PK976_04080"
                     /inference="COORDINATES: protein motif:HMM:NF020442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FRG domain-containing protein"
                     /protein_id="HRC67097.1"
                     /translation="MKTDLIAKNWVHLQDLLFRDTWDKDLKRYRSAYVYRGLSDKNYQ
                     LVSSLIRLGGPYDRLERHLLRNFIKYARRGSTGESLWNWLVLAQHHGLPTRLIDWTYS
                     PLVALHFATANPLKYKDDGVIWCVNYVKANHFLPQSLLDIILEEGSNVFTPYMLDKVC
                     NSLSKLSELQSEPFNIFLEPPSLDERIVNQHALSSLMSTAKAHLGVWLQNHLDIYFRI
                     IIPAELKWEIRDKLDQSNITERVLFPGLDGLSLWLKRNYSPKDIPFDI"
     gene            complement(975..1655)
                     /gene="rsmI"
                     /locus_tag="PK976_04085"
     CDS             complement(975..1655)
                     /gene="rsmI"
                     /locus_tag="PK976_04085"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006256326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HRC67098.1"
                     /translation="MSKLYLVPTPIGNLEDITLRALRILKEVNFILAEDTRSSRILLQ
                     HYQIQTPLVSHHKFNEHQSLRKVIERLRNGEMAALISDAGTPAISDPGFLLVRECIRE
                     NISIECLPGPVAFIPALVESGLPCDRFYFEGFLPVKKGRKTRMARMAQRDETTIVYES
                     PYRIVRTLKELSEILGMERKASISREISKIFHETIRGTFLELIEWAKSKPLKGEFVIV
                     IGGTKDEN"
     gene            1782..1855
                     /locus_tag="PK976_04090"
     tRNA            1782..1855
                     /locus_tag="PK976_04090"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1816..1818,aa:Ala,seq:ggc)
BASE COUNT          640 a          416 c          385 g          587 t
ORIGIN      
        1 cttcagaaag attctcgtcc atgttgataa atcctcctgg taaagcccac atgccttcga
       61 aaggaggatt tttacgttgt atcagtaaaa tctcctcctt gtcgtgatgt ttacgaaata
      121 aggcaacatc aacggtaaca cagggtcgtg gatattcata agtgtatgac attttcaaaa
      181 ataactaaat gtcgaaagga atatcttttg ggctataatt ccgtttaagc catagactca
      241 gtccgtcgag gccagggaaa agaactcttt cggtaatatt cgactgatcg agtttatccc
      301 tgatttccca tttgagttcg gcaggaataa tgatgcggaa gtaaatatcg aggtgattct
      361 gcaaccaaac acctagatga gcttttgcag tcgacatgag cgaagaaaga gcatgttgat
      421 tcactatgcg ctcatcgagt gaaggaggtt caagaaaaat attgaagggt tccgactgta
      481 actcactgag tttactcaat gaattacaaa ctttatcgag catgtaagga gtaaatacat
      541 tcgatccttc ctcgagaatg atatcgagca agctttgcgg caaaaaatga ttggctttca
      601 cataattgac acaccatatc acaccgtcat ctttatattt caaaggattg gccgtagcaa
      661 aatgcaaggc taccagaggc gaataagtcc agtcgatcaa acgggtgggc aaaccatgat
      721 gttgagccaa tacgagccaa ttccacaaag actcgcctgt tgagccacga cgggcatatt
      781 taataaaatt tcgcagcaga tgccgttcga gcctatcgta aggcccacca agcctaataa
      841 ggctggatac aagctgataa ttcttatcgg ataaaccccg ataaacataa gctgaacgat
      901 atcgcttcaa atctttatcc caggtatcac gaaaaagcaa atcctgaaga tgaacccagt
      961 ttttggctat taagtcagtt ttcatctttt gtgcctccta taacaataac aaattcccct
     1021 tttaaaggtt tggattttgc ccattctatg agttccaaaa atgtgcctct aatggtttca
     1081 tggaatatct tgctgatttc gcgcgaaata gaggccttcc tttccattcc taaaatctca
     1141 gaaagctcct ttaaggtacg aactattcgg taaggagact cataaacaat agtagtttca
     1201 tctctctgtg ccatacgtgc cattcgtgtc ttccttcctt tttttactgg caggaaacct
     1261 tcgaaataaa acctatcgca aggtaagccc gactcaacca gcgcaggtat aaaagctacg
     1321 ggacccggaa gacattcaat cgaaatattt tctctaatgc attctctcac caataaaaat
     1381 cctggatcgg aaatagccgg agttccagca tcggaaatca aggctgccat ttccccattt
     1441 cttagccttt ctattacctt tctcaatgat tgatgttcgt tgaacttatg atgagaaacc
     1501 aatggggttt gaatttgata atgttgtaat aatattcgtg atgaacgcgt atcttcggcc
     1561 aatataaaat tgacttcttt aagtatacgc aatgccctca gagtaatgtc ctcgaggtta
     1621 ccaataggtg ttggcaccag gtataatttc gacataatct acctttgata aattttaaaa
     1681 agcaagcctt ctaatatcat cgacaatatg gtaaaggatt tttcataaaa ttaagcaaag
     1741 aaataaaaaa aatagcttac ttttgcatgc cataaaacat tggggcgtta gctcagttgg
     1801 ctagagcgtt agactggcag tctaaaggtc aggggttcga ctcccctacg ctccacatca
     1861 gaccacagag aaaattctgt ggcttttttt tggaaaatct attcccaaat aaaaatttca
     1921 tttacccgag ggttttttca tcattcatgg tcaaatagaa taacccaatt aaaattttaa
     1981 ataagatgaa atccaaaatg atcattttat gtgtaacgat gatgagct
//
LOCUS       DAORPD010000279         2026 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_10234_length_2026_cov_2.768645, whole genome shotgun sequence.
ACCESSION   DAORPD010000279 DAORPD010000000
VERSION     DAORPD010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10234_length_2026_cov_2.768645"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..424)
                     /gene="gcvH"
                     /locus_tag="PK976_04095"
     CDS             complement(41..424)
                     /gene="gcvH"
                     /locus_tag="PK976_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008661521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HRC67099.1"
                     /translation="MNILDNLKYTRDHEWIKVEGNSAWIGISDYAQSQMGDIVYVEVE
                     TEGEILNKGESLGSIEAVKTVEDIYMPVSGEIVEFNEKLIEKPDLLNKDPYGEGWIVK
                     ITLINPSELDDLLDSQSYKEFIESL"
     gene            complement(585..1154)
                     /gene="gmk"
                     /locus_tag="PK976_04100"
     CDS             complement(585..1154)
                     /gene="gmk"
                     /locus_tag="PK976_04100"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HRC67100.1"
                     /translation="MNRKMLIFSAPSGGGKTTIVKAVLKQFPELAFSVSTTSRPQRPN
                     EINGVDYYFISTEEFRKKIANEEFIEWQEVYPGIYYGTLKSELERIWALGKNPVFDID
                     VKGALNLKNIFQSESLTIFIQPPSLQILEQRLRNRGTETEESLARRLGKAAYELTFAT
                     YFDAVIVNDQLQQAIQEAIHLVADFLHSI"
     gene            complement(1159..>2026)
                     /locus_tag="PK976_04105"
     CDS             complement(1159..>2026)
                     /locus_tag="PK976_04105"
                     /inference="COORDINATES: protein motif:HMM:NF015699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1732 domain-containing protein"
                     /protein_id="HRC67101.1"
                     /translation="MTGLGKTQVLAKGKNITIEVRSLNSRQADLNLKLPVIYREKEYE
                     IRNTVIDSLERGKIDVSINVDFTNSSHAAILNMAVAKNYFDQLKTLEKELGLDRVQTD
                     YIGIISRMPEVFRMIDSEINQEEWDLLIGGLKDALCQVIGFRKEEGSVLEKDIGQRIG
                     CIEKLLQSIQPYEEERIDLVRNKILKELMALPIQPSYDKNRFEQEMIYWLEKIDFTEE
                     KTRLSKHLHHFLRTLEEESSQGKKLGFIAQEIGREINTLGSKANHAEIQRIVISMKDE
                     LEKIKEQLLNVL"
BASE COUNT          572 a          437 c          368 g          649 t
ORIGIN      
        1 aaattcaacc ccatagaata ggataacaag aagctctggt tcagagagat tcgatgaatt
       61 ccttataaga ctggctgtcg agcagatcat ccagttcgct ggggttaatc agagtgattt
      121 ttacgatcca gccttcgcca taagggtctt tattcaacaa atcgggtttt tcgatgagtt
      181 tttcattaaa ctcgacaatt tcacccgaaa cgggcatata aatatcttcg acagttttta
      241 ctgcttcgat gcttccgagg ctttctcctt tgtttagaat ttcaccctca gtttccacct
      301 ctacataaac aatatcaccc atctggcttt gggcataatc ggaaatacct atccatgccg
      361 aattgccttc caccttaatc cattcatgat cacgagtgta ctttaggtta tctaatatgt
      421 tcattttatt gttttttgat tttcgatttg cttgcaaata taaagttttc tttcaagtgg
      481 tgatacaaga aataaaattt cccaaaaatc aaataaagaa gcatgaaagg ttcaaaactt
      541 cataaacata atttctgtaa tctgtagaaa aaacaaattt ggattcaaat ggaatgcaag
      601 aaatctgcga ccaggtgaat ggcttcctgg atagcttgct gcaattgatc gttcacgata
      661 acagcgtcga agtaagtggc gaaggtaagc tcgtaggcag ccttgcccaa acgtcgagca
      721 agcgattctt cggtttcggt gcccctgttg cgcagcctct gttccaaaat ctgtaacgag
      781 ggtggctgaa taaaaatggt caaggattct gattggaaaa tattcttcaa attgagagct
      841 cctttcacat caatgtcgaa cacaggattt ttgcctaggg cccaaattct ttctaactcg
      901 cttttcagag ttccatagta aatcccggga taaacttcct gccattcgat gaattcctcg
      961 tttgcaattt tctttcgaaa ttcttcagta ctgatgaaat aataatccac accattgatt
     1021 tcatttgggc gctgtggccg gcttgtggtg gaaaccgaaa aagccagttc ggggaattgt
     1081 ttcaaaacag ccttgacgat ggtcgtttta cctccacccg aaggagctga aaatatcagc
     1141 atcttcctgt tcatgacttc ataagacatt caaaagttgt tccttgatct tttcgagttc
     1201 atctttcata gatatgacga ttctctgtat ctcggcatga ttggccttcg atcccaatgt
     1261 attgatttct cgcccaatct cctgggcaat aaaaccaagt tttttcccct gtgatgattc
     1321 ttcttcgagc gttcgaagaa aatgatgcag atgcttggaa agacgggttt tttcctctgt
     1381 aaaatcaatt ttctcgagcc aataaatcat ttcctgctca aaacggtttt tatcataaga
     1441 tggctgaatg ggcaaagcca tcaattcctt caaaattttg ttgcgcacca aatcgatgcg
     1501 ttcctcctcg tagggttgaa tgctttgaag caatttttct atacatccaa ttcgctggcc
     1561 aatatctttt tcaagaaccg aaccttcttc ctttctaaag ccaattactt gacacaaggc
     1621 atccttcaga cctcctatca aaagatccca ttcttcttga tttatctcgg aatcaatcat
     1681 tctaaatact tcaggcattc gcgaaataat accaatataa tcggtttgaa ctctatcaag
     1741 gccgagttcc ttttcgagag tttttaactg atcgaaataa tttttagcca cggccatatt
     1801 caggatagct gcatgtgaag aattagtaaa gtcaacattg atagaaacat cgattttccc
     1861 tctttcgaga gaatcaatga cggtatttcg aatttcatat tctttttcgc gatatatcac
     1921 aggaagtttc aagtttaaat cagcttgcct gctattgagt gaacggacct cgatggtaat
     1981 gttttttccc ttggccaaaa cctgagtttt ccccaaaccc gtcatg
//
LOCUS       DAORPD010000280         2023 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_10258_length_2023_cov_2.538618, whole genome shotgun sequence.
ACCESSION   DAORPD010000280 DAORPD010000000
VERSION     DAORPD010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10258_length_2023_cov_2.538618"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..638
                     /locus_tag="PK976_04110"
     CDS             171..638
                     /locus_tag="PK976_04110"
                     /inference="COORDINATES: protein motif:HMM:NF015581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HRC67102.1"
                     /translation="MANKIPDIEPDIEDEQFFFIFAINYVTMNYYFTKTLNCSFEEAE
                     SRVHEGLSKVGMGIVMEIDFREKFKEKLNVDFRPYKVLGACSPGHAYRSILKEDKLGV
                     LLPCNVLIQQIGDKLTEVSVMDPEGAMMAVNNPEVKCITDEVRIKLQHFIEEL"
     gene            818..1810
                     /locus_tag="PK976_04115"
     CDS             818..1810
                     /locus_tag="PK976_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRC67103.1"
                     /translation="MQSIDIRELNERIQQESAFVDIIRMEMGKVIVGQKQMVERLLIG
                     LLSNGHILLEGVPGLAKTLAIKSLANIIDAKYSRIQFTPDLLPADLTGTLIYSQKSEE
                     FLVRKGPIFAHFILADEINRSPAKVQSALLEAMQERQVTIGEHTFKLEEPFMVLATQN
                     PIEQEGTYPLPEAQVDRFMLKVVITYPDKEEEKLILRQNIRNEEVFTETRLKPEDIIK
                     ARSLVREVYLDEKIENYITDIVFATRFPEHYKIDSFKGLISYGASPRASINLALASKA
                     FAFIRRRGYVIPEDVRAVAHEVMRHRIGLTYEAEAENITTEEIISQILNTVEVP"
     gene            1855..>2023
                     /locus_tag="PK976_04120"
     CDS             1855..>2023
                     /locus_tag="PK976_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012055625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRC67104.1"
                     /translation="MDTSEIFKKVRKIEIKSRGLSNQIFSGEYHSSFKGKGMEFSEVR
                     EYQYGDDIRNID"
BASE COUNT          653 a          383 c          408 g          579 t
ORIGIN      
        1 agggatacat ctttattaga tttttcgata caaagataag gcattttttt caaaagtcaa
       61 caccttttca aaaatttaac aaaatttaat atgagaaact ttaatattgg gttgttaaga
      121 ttcttttcgg atgaaacgca atggaagcag aaaatcattt cccaggcttt ttggcaaata
      181 agattcctga tattgaacct gatattgaag atgagcaatt tttctttatt tttgcaataa
      241 attatgtaac catgaactat tattttacca aaactttgaa ttgttctttt gaagaagccg
      301 aatcgcgtgt tcatgaaggg ctatcgaaag taggaatggg tatagtgatg gaaatcgatt
      361 ttcgtgaaaa attcaaggaa aagctcaatg tcgatttcag gccttataag gtcttaggag
      421 cttgttcacc cggtcatgct tacagatcta tcctcaagga agacaaactg ggtgttttgc
      481 tgccctgcaa tgttcttatt caacaaattg gggataagct gaccgaagtt tcggtcatgg
      541 accctgaagg agccatgatg gccgtgaata accccgaagt aaaatgcatt accgacgaag
      601 tgaggataaa gctgcaacat ttcatcgaag agctttagat agaaaatagt ttgtctcgag
      661 tcctcgaaat gtcgagcgaa aaggagattt tttataactt cttcataatc acttaaatta
      721 acatttttta acgacttatt taacactatt taacccaatg aaagttgttc agcccgaaag
      781 aggctgctaa ttttgccaca tcaaaatata aaatgatatg caaagcatag atataagaga
      841 acttaacgag cgtattcagc aggaaagtgc gtttgtcgat atcatccgaa tggaaatggg
      901 aaaggtcatt gtaggtcaga agcaaatggt agagcgatta ttaattggat tgctcagtaa
      961 cgggcacatt cttctggaag gtgttcctgg acttgcgaag acattggcca tcaaatctct
     1021 ggcaaacatt attgatgcga aatacagccg cattcaattt acgcccgatt tattacctgc
     1081 cgaccttacc ggtacgttga tttacagtca gaaaagcgaa gaatttcttg tccgtaaagg
     1141 acccattttt gcccacttca tactggccga tgaaattaat cgttcgccag ccaaggtaca
     1201 gagtgcgctg ctcgaagcca tgcaagaacg tcaggttact ataggggaac atactttcaa
     1261 gctcgaagaa ccctttatgg tattagctac tcagaatccc atcgaacagg aaggtactta
     1321 tcctttgccc gaagcacaag ttgatcgttt catgctaaag gtagtgatta cttatcccga
     1381 caaggaagaa gaaaaactca tcttaaggca aaacattcgc aacgaggagg ttttcaccga
     1441 aacacggctc aaacccgagg acatcatcaa ggcacgtagc ttagtaagag aagtctatct
     1501 ggacgagaaa atcgaaaact acattactga catcgtgttt gccacacgtt ttcccgaaca
     1561 ttacaaaata gatagtttca aaggcctgat ctcttatggg gcctccccac gtgcaagtat
     1621 taatctggct cttgcatcaa aagcctttgc attcattaga agacgtggct atgttattcc
     1681 agaagatgtt cgcgccgttg ctcacgaggt gatgcgtcat cgcattggac taacctacga
     1741 agccgaagcc gaaaacatca ctaccgaaga aattattagc cagatactga ataccgtcga
     1801 agtaccctga taattgaatc tattattttc ttatcacatt ttaatttttt tacgatggat
     1861 acttctgaaa tcttcaaaaa agtcagaaaa attgaaataa aaagccgtgg gctttccaat
     1921 caaattttct ctggagaata tcacagttct ttcaagggga aaggaatgga atttagcgaa
     1981 gttcgtgaat atcaatatgg cgatgatatc cgtaatatcg att
//
LOCUS       DAORPD010000281         2023 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_10259_length_2023_cov_2.307927, whole genome shotgun sequence.
ACCESSION   DAORPD010000281 DAORPD010000000
VERSION     DAORPD010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10259_length_2023_cov_2.307927"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(402..1127)
                     /locus_tag="PK976_04125"
     CDS             complement(402..1127)
                     /locus_tag="PK976_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRC67105.1"
                     /translation="MEEVIRFEDAAIFQRDILVLNGIDLVVKPGEFVYFIGKTGSGKS
                     SLLRTIYADLPLRAGKGWVAGFDLTRLRKRQLPYLRRKIGIVFQDFQLLSDRTVAENL
                     LFVMKATGWKDKKQMKQHIEELLSKTGIPTKGFKMPHQLSGGEQQRVGVARALINQPE
                     ILIADEPTGNLDPESSDGILQLIQEINRSGTTVIMATHNYRLIRDFPGRVIRLEQGKL
                     LETPQTMEEENITENPDNLDNEP"
     gene            1518..1733
                     /locus_tag="PK976_04130"
     CDS             1518..1733
                     /locus_tag="PK976_04130"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC67106.1"
                     /translation="MYLDKESCHIHFGLGARQIQLYNLQGKVIREWLVPQGCIFMDVS
                     LKNLPTGIYLFRVTENQGVKGMRVVKI"
BASE COUNT          570 a          456 c          339 g          658 t
ORIGIN      
        1 ttctcgagaa attaaattca gacaacacat cctatcacac tctattgatt tgcatatatt
       61 tttaaattca aacctttaac cttttcctac tattcaccaa ccaatcctct aaacattaaa
      121 tcccaaatta caactaacaa cgaacaactt accaaattac aaatttcaat ctctgtcaca
      181 ccgtctcctt gtctcttcgt cttccttcct tccttccttc cttcctccat tttcaacttt
      241 atgaactttt aactcatcca tccaccatcc accaatccag cttgtctccc ggtctcctcg
      301 tcccgcagtc tcctcgtctc ccttcctccc ttatgaactt ttaacttacc aatcaccatc
      361 tcctaaatac tagttacaaa ataaaagttg ttttttatgc ttcacggctc attatcaaga
      421 ttatcagggt tttctgtgat attttcttct tccatagttt gaggcgtttc gaggagtttt
      481 ccctgttcga ggcggattac gcgtcctggg aaatcgcgaa taagccggta attgtgagtg
      541 gccatgatga cagtggttcc acttcggtta atctcctgaa ttaactgtag aatgccgtcc
      601 gacgattcag gatcgaggtt gccggtgggt tcgtcggcga tgaggatttc gggctgattg
      661 atgagtgctc gggctacacc cacgcgttgt tgttcccctc ccgagagttg atggggcatc
      721 ttaaagcctt tggtagggat tccagtcttt gatagtaact cttctatatg ttgtttcatc
      781 tgtttcttat ctttccagcc cgtagccttc attacaaaaa gcagattttc ggccacagtt
      841 ctgtcggaaa gcaactgaaa atcctgaaaa acgattccta tctttcgacg tagataaggt
      901 agctgacgct ttcgaagccg agttaaatcg aaacctgcta cccatccctt gccagctcga
      961 agtggcaaat cggcataaat ggtcctcaac aaactcgact tgccactccc tgtcttccca
     1021 atgaaataca cgaattctcc cggttttact accagatcga ttccgttgag cacaagaata
     1081 tccctctgaa atatggccgc atcttcgaaa cgaatcactt cctccattaa aatcggtata
     1141 ttttaaataa ttatcaccta caaagatatc agtctttttt gattgttctt tgtattaaac
     1201 aatcatattt ggatgtgaat ttttctcaat cgctcttgtt tgaggagatt tggcaatcaa
     1261 accatgagca ataacccaaa actttctatc aaagcttagc ctgaaaaaat tacttccctc
     1321 caacttctat ctctttttat cccgaaaatt ttctctaatc gaaagtttat cagccagcta
     1381 tgcattttct atggatagcc gcagaattcc cgtttgctcc ttgtaatggg ttcttctcta
     1441 ttggaaaatt atttatcatt aggttttaaa atttatttat taaggatcat cattaagcaa
     1501 aagatagaaa actggatatg tatcttgata aagaaagttg tcatattcat tttggtcttg
     1561 gagcaaggca aattcaacta tacaaccttc aaggtaaagt aataagagaa tggcttgtac
     1621 ctcaaggttg tattttcatg gatgtgtcgt taaaaaatct tccaacaggg atttatctat
     1681 tcagagtaac tgaaaatcag ggagtgaaag gcatgagagt agtaaaaata tagaacaatg
     1741 attaaaatca ataacatggc atatcactct tatgtgagag gagttttaag aacttgattt
     1801 cggtaaattt tgcaaagtcc agtaaatttg tcagcacaat aatgttatat gaagggtcat
     1861 tttcttgggt tatttattat tccgctggca attgtagtta cctcgtgccg taccttggct
     1921 cccgaaaagc ctatcgaatc gtatcccatt gcaaggcaac aggctcgctc atcttttatt
     1981 caggtaaaca tcccgctcga aggtgtagta atccaacaaa tca
//
LOCUS       DAORPD010000282         2015 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_10317_length_2015_cov_2.224490, whole genome shotgun sequence.
ACCESSION   DAORPD010000282 DAORPD010000000
VERSION     DAORPD010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10317_length_2015_cov_2.224490"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="PK976_04135"
     CDS             <1..376
                     /locus_tag="PK976_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67107.1"
                     /translation="VFGEPDVDVKTLPDGQLTVTIKGVDIYDPTTGEIRSSSTDDIAC
                     WFIDSNYNGESFFVRHAYFTGADQPYDKLKRALRAEVDESAWSALYSTTSYPFEKPQT
                     GKIAVKVINHYGDEVLKVYECH"
     gene            415..891
                     /locus_tag="PK976_04140"
     CDS             415..891
                     /locus_tag="PK976_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67108.1"
                     /translation="MNLKELSNDDLLTLRAEVEKELHARGILFSVGETGEKIAIEFFC
                     STPGLPNLIYAPTGAKNVDALSRDGDRYSIKTIQYAKKTGTIYPDTKNPEKPLFEYLL
                     IVILSTNYELRAVYRFSWEQFCVVRAWDKRMNAWYVPVSRKRLDQGEEIISIKKDS"
     gene            947..1405
                     /locus_tag="PK976_04145"
     CDS             947..1405
                     /locus_tag="PK976_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67109.1"
                     /translation="MDIVKVFEDSSEIIDSETHVEQESNKVLSKIRDGLINIGFQVEA
                     GKKADEKINVPVLFGKNGKIVRSFDADAYCEENGFVIEVEAGRGVTNYQFLKDLFQAC
                     MMHEVNYLAIAVRNKYRNNRDYEKVQTFFETLYASQRLQLPLKGILVIGY"
     gene            1464..>2015
                     /locus_tag="PK976_04150"
     CDS             1464..>2015
                     /locus_tag="PK976_04150"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HRC67110.1"
                     /translation="MIADPSRSGPIIAILPMTDGQMRYRVFHSPTDIREYLADQIEVV
                     DITPTGDRLVDALQREEWLPASEFQARLTAARLADPQIDHLYSLYSGRIQHIPFQFKP
                     LLRFIRADQPRLLIADEVGVGKTIEAGLILKEFQSRQRVDNVLVLCPKALVGKWREEM
                     RRFDEDFRPLSAENLRYCLEETHL"
BASE COUNT          586 a          399 c          470 g          560 t
ORIGIN      
        1 ggtctttggc gagccggatg tggacgtcaa aacattgccc gatggccagt tgacagtcac
       61 catcaagggc gtagatattt acgaccccac cactggcgag atccgctcca gctcaaccga
      121 tgatattgcc tgctggttca tcgattcaaa ttacaacggc gagagtttct ttgtgcgcca
      181 tgcctacttc actggtgctg atcaacctta cgataaactg aagcgtgccc tgcgcgccga
      241 agtggacgaa tccgcatgga gtgcacttta ctcgaccacc tcctatccat ttgaaaaacc
      301 ccagaccggc aagatcgcgg tcaaggtgat caatcattat ggggatgagg tgttgaaggt
      361 gtatgaatgt cattagcttt ttaaaaagta tctatataca caatagaagg caggatgaat
      421 cttaaagaac taagcaatga tgatcttctt actttacgag cagaagttga aaaggaactg
      481 catgctcgag gaattttatt ttcagttggt gagacaggtg aaaagattgc tatagagttc
      541 ttttgctcaa ccccgggctt gccaaattta atatatgctc caactggggc aaaaaatgta
      601 gatgcattat cgagggatgg agatcgatac tcgataaaga cgattcaata cgcaaagaaa
      661 accggaacca tttatcctga taccaaaaac ccagaaaaac cattatttga ataccttttg
      721 atcgtcatac tttcaactaa ttatgagctg cgagcagtat atcgatttag ttgggaacaa
      781 ttctgcgtgg tacgagcatg ggataagaga atgaatgcat ggtatgttcc ggtcagtaga
      841 aaacgtcttg atcaaggcga agagattatc tcaataaaaa aggatagtta gttttatgat
      901 caattggatt tattttccac taagtgatcg ccctccacag aaggtattgg atatcgttaa
      961 ggtattcgaa gattcctcgg aaattattga ttctgaaaca catgtagaac aggaaagtaa
     1021 taaggtcctt tccaagatac gtgatggctt aatcaatatt ggttttcaag tcgaagcagg
     1081 aaaaaaagct gacgaaaaaa tcaatgtacc agttctgttt ggtaaaaacg gcaagattgt
     1141 gcgatctttt gatgctgatg catattgcga agaaaatgga tttgtaatag aagtcgaggc
     1201 aggtcgtggt gttacaaatt accaattttt gaaagattta ttccaggcat gtatgatgca
     1261 tgaggttaat tatctggcta ttgctgtgcg taataaatat cgaaacaatc gcgattatga
     1321 aaaggtacag actttttttg agacattata tgccagtcaa agattacaat tgcctctgaa
     1381 agggatatta gtaataggtt actagatttc tacaataagg atttctcatg agctattctt
     1441 tcgccattgg gcaaatcgtt cgattgattg cagatccttc ccgcagtggg ccgattattg
     1501 cgatactccc aatgacggat gggcagatgc gctatcgcgt gttccattct ccaacagata
     1561 tacgggagta tctggctgat cagatcgaag tggtggacat tacacccact ggtgatcgat
     1621 tggtcgatgc cctgcaacgc gaagaatggt tgccggcttc agaattccag gccaggctta
     1681 ctgccgcccg tctggcagat ccgcagatcg atcacctgta ttcactctat tccgggcgaa
     1741 ttcagcatat cccatttcaa tttaagccac ttctgcgttt tatccgtgcc gatcagccac
     1801 ggcttctgat tgcggatgaa gttggcgttg gaaaaacaat cgaagccggg cttattctca
     1861 aagagtttca atcccggcag cgagtggata atgtgttggt tctttgtcct aaagccctgg
     1921 ttggaaaatg gcgagaagaa atgcgccgtt ttgatgaaga ttttcggcct ctcagtgctg
     1981 agaatttacg gtactgcctt gaagaaacgc acctg
//
LOCUS       DAORPD010000283         2014 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_10330_length_2014_cov_2.046963, whole genome shotgun sequence.
ACCESSION   DAORPD010000283 DAORPD010000000
VERSION     DAORPD010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10330_length_2014_cov_2.046963"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="PK976_04155"
     CDS             <1..253
                     /locus_tag="PK976_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRC67111.1"
                     /translation="GYLYVLSIRKNWLKKLKLKVLFKPKPKAKIKYRPGSEKEEKEYF
                     EDKKHMEEEIDRILDKIKLTGYQGLTAKEKETLFKYSKK"
     gene            317..1450
                     /locus_tag="PK976_04160"
     CDS             317..1450
                     /locus_tag="PK976_04160"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRC67112.1"
                     /translation="MTRKKKSKKHKLVGGFLRLISLLIAIGLIISCIAQFVPPDKFWP
                     PAFFGLSFLIWFVANLFFLIFWIIRRARFAWIHVACLILSLHLFSHFIPLKGSKSSDK
                     LPASISLLSYNIKGLAGVNSIKSSGLSDSLFNFIKNENANIVCLQEYTERRRENKPPA
                     ELVFKSMDLDHFAKSAYFKNKEVARLFIATFSQFPICNKIEVHSDHRLIALITDIVVE
                     KDTFRVFNVHLQSISFNDENYRFLMEAQQNISSLQSDSMQQDSKKIFWKIRTGFIKRA
                     GQARFLREIIETSPYPVVVCGDFNDTPASYAYHTIKRGLTDAFMEKGKGIGNTYFGNL
                     PKIRIDYVLLHPKFQCQRFEIIKQTISDHHPVKAVFYKKKYDE"
     gene            1450..>2014
                     /locus_tag="PK976_04165"
     CDS             1450..>2014
                     /locus_tag="PK976_04165"
                     /inference="COORDINATES: protein motif:HMM:NF021210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2400 domain-containing protein"
                     /protein_id="HRC67113.1"
                     /translation="MEKGKYFLDLKFFLDEAAEFFNCPEFIPEDPICIPHRYQMREDI
                     EISAFLAAIMAWGNRKTIITKASELLERMHNHPFEFIMDDNPKKYKFVEGFKHRTLND
                     EDIIFLLEGLHFIYKKHQSPEKLFMPRQNEPDTSGGIQRFVELMLQSPHSKRSLKHLP
                     NIEKGSAAKRINMFLRWMVRKDDKGVDF"
BASE COUNT          670 a          391 c          371 g          582 t
ORIGIN      
        1 tggctacctc tatgttttat ccattcgtaa aaactggctg aaaaaactaa aactgaaagt
       61 attgttcaaa cccaaaccaa aagcgaaaat taaatacagg ccaggaagcg aaaaggaaga
      121 aaaagagtat ttcgaagata aaaaacacat ggaagaagaa atcgatcgta ttctcgataa
      181 gattaaatta accggatatc aaggactaac tgctaaagaa aaagaaacct tgttcaaata
      241 cagtaaaaag taaaaacgta gattctatca tacaaattca tagattcttc caaattatta
      301 aaacacgata agaataatga cgaggaaaaa gaaaagcaag aagcacaaac tggtaggggg
      361 cttccttcgt ttgattagcc tattgattgc tattggattg attataagct gtatagctca
      421 gttcgttcct cccgataaat tctggcctcc tgcattcttc ggactttcat tcctcatctg
      481 gtttgtggct aacctgttct tccttatttt ttggatcatt cgcagggctc gttttgcatg
      541 gatacatgta gcatgcctta ttctttctct tcatctcttc tcgcatttca tcccattgaa
      601 gggttccaaa agctccgaca agctgcctgc ttctattagc ttgctcagtt ataacatcaa
      661 aggacttgca ggtgtaaact caattaaaag ttcaggctta tccgactctt tgttcaattt
      721 tattaaaaat gaaaatgcca acattgtttg tttgcaggaa tataccgaaa ggcgaaggga
      781 aaacaagcct ccagcggaac tggttttcaa atccatggat ttggatcatt tcgccaaatc
      841 ggcctatttt aaaaataaag aagttgcccg tttgtttata gctaccttta gtcaattccc
      901 gatttgcaac aaaatagaag tccacagtga ccatcgattg atagcgttaa ttactgatat
      961 tgtggtcgaa aaagatactt tccgtgtatt taatgttcat ttacagtcta tttcgtttaa
     1021 cgatgagaat tacaggtttt taatggaagc tcaacaaaat atttcttcac ttcagagcga
     1081 ttccatgcag caagattcca agaaaatatt ctggaagatt cggactggat ttatcaaacg
     1141 cgctggtcag gctcggttct tacgtgaaat aatcgaaact tcaccatatc ctgtggtggt
     1201 ttgcggcgat ttcaacgaca ctccggcatc ttatgcctac catactataa aaagaggact
     1261 cacagatgcc tttatggaaa aaggaaaagg cattggcaac acctattttg gaaatcttcc
     1321 taaaattcga attgattatg tgctgctaca tcccaaattt caatgccaga gattcgaaat
     1381 catcaaacaa accatttcgg accatcatcc tgtaaaagca gtcttttaca agaagaaata
     1441 cgatgaataa tggaaaaggg gaaatatttt cttgatttga aatttttttt agatgaggct
     1501 gctgaatttt tcaattgccc agaattcatt ccagaagacc caatttgcat tccacatcgt
     1561 taccaaatgc gagaagatat agagataagt gcatttttag ctgccatcat ggcttgggga
     1621 aatcgaaaaa ccatcatcac aaaagcgtca gaactgctcg agcgcatgca caatcatccc
     1681 tttgaattca ttatggacga caatcccaaa aaatataaat ttgtcgaagg cttcaaacac
     1741 aggaccctga atgacgaaga tataatcttt ttgttagaag ggcttcattt catctataag
     1801 aaacatcaaa gtcctgaaaa acttttcatg cctcggcaaa atgaacccga cacatcaggt
     1861 ggtattcagc gatttgtgga gctgatgctg caaagcccac attctaagcg ctctctcaaa
     1921 catttaccaa acatcgaaaa gggctcggct gcaaagagaa tcaacatgtt tttaagatgg
     1981 atggttagaa aggacgacaa aggtgttgat tttg
//
LOCUS       DAORPD010000284         2009 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_10366_length_2009_cov_2.293245, whole genome shotgun sequence.
ACCESSION   DAORPD010000284 DAORPD010000000
VERSION     DAORPD010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10366_length_2009_cov_2.293245"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1142
                     /locus_tag="PK976_04170"
     CDS             <1..1142
                     /locus_tag="PK976_04170"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HRC67114.1"
                     /translation="IGVLIIFFVLILDDVIRSYKSYYQSFLVLFVLHFSFTYSARFLL
                     TTITNNKIHRRKIGFNTVMVGCNGNALSIYQEIENQPLSPGNKFVGFVDAGVSEKNLL
                     DGYMPHLGSYKDLKRIIDEYKIEEVIIAFERSEKDTVERVITELEDANVVIKIAPIMQ
                     DILLGSVRTSSIYSPLIEISTDLMPAWQMVLKRAMDIVVSLIAIIVLSPVFLFTAIGV
                     KLSSPGPILYSQPRVGLRGKTFKMVKFRSMYQDAESNGPQLSSKNDPRVTPFGRIMRQ
                     YRLDEIPQFFTVLKGDMSIVGPRPERSYYIEQIIQKMPHYKLLLKVKPGITSWGQVKF
                     GYAENLDQMTERAKFDLIYLDNMSIAMDIKILVYTIMIVLQGRGK"
     gene            complement(1149..1733)
                     /locus_tag="PK976_04175"
     CDS             complement(1149..1733)
                     /locus_tag="PK976_04175"
                     /inference="COORDINATES: protein motif:HMM:NF025492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HRC67115.1"
                     /translation="MKKSIVTGILLLCTAMTIEAQTSPQIVVRATIFENDTIPLINLN
                     PVTIYGLPVFKNKKDQRQWEKLVRNVKKVYPYARLAGIKFQEYENLILTSKSEKERKK
                     YVNRLEKELTEQYSDELEKLTFTQGKILFKLLDRETGNSSYELVKELKGKFMAFFWHS
                     FARIFGYNLKEKYDPYGKDATIEQIVVLIENGAL"
BASE COUNT          612 a          361 c          384 g          652 t
ORIGIN      
        1 tgataggggt tctaattatt ttctttgttt tgattcttga tgatgtaatt cgttcttata
       61 aatcctatta tcaatcattt ttagttttat tcgttttgca tttttcattt acttattcgg
      121 cccgattttt actaactact attactaaca acaagattca tcgacgaaag attggattca
      181 atacggttat ggtgggctgc aatggcaatg ccttatccat atatcaggaa atcgaaaacc
      241 agcctttgtc gccaggcaac aaatttgttg gatttgtcga tgcgggggtt tctgaaaaga
      301 atttactcga tggttatatg cctcatttgg gaagctataa ggatctgaaa agaataatcg
      361 atgaatataa gattgaggaa gtaatcattg cattcgagcg atcggaaaaa gatactgtcg
      421 aacgggtaat taccgaactg gaagatgcca atgtggtgat aaagattgct cccatcatgc
      481 aggatattct tctggggtcg gttcgtacct cctccattta ttcacccttg attgaaattt
      541 cgaccgattt gatgcctgca tggcaaatgg tattgaaacg agccatggat attgtcgttt
      601 cattgattgc gattattgtt ctttctcccg tatttttatt cacagccatt ggagtaaaac
      661 tttcgtcgcc gggtcctata ctctatagcc aaccacgagt aggactgaga gggaaaacat
      721 ttaaaatggt caaattccgt tctatgtatc aagatgccga aagcaatggt ccacaattat
      781 cctcgaaaaa cgatccgcga gtaacacctt ttggaagaat catgcggcaa taccgattgg
      841 acgaaatccc tcagtttttt acagtactga aaggtgatat gtccatagta ggtccgaggc
      901 ccgaacgttc ttattacatc gaacagatca tccaaaaaat gcctcattac aaactgttac
      961 tcaaagtaaa accaggaata acgagttggg gacaggtaaa atttggttat gccgaaaacc
     1021 tcgatcagat gaccgaaagg gcaaaattcg atctcatata tctggataat atgtcaattg
     1081 ccatggatat taagatactc gtctatacca tcatgattgt tttacaggga agaggaaagt
     1141 aattgctctc aaagagcacc gttttcgatt agaaccacga tctgttcgat agtggcatct
     1201 tttccgtaag ggtcgtattt ttctttcaaa ttatatccaa aaatccgggc aaatgaatgc
     1261 cagaaaaaag ccataaattt tcccttgagt tcttttacga gttcatacga agaattaccc
     1321 gtttctctat ctaatagttt aaatagaatc ttcccttgtg taaaggtgag tttttccaat
     1381 tcgtcggaat attgttcggt tagctctttt tctaaacgat tgacatattt tttccgttcc
     1441 ttttccgatt tagaggtcaa aatgagattt tcgtattcct gaaatttaat gcctgccaat
     1501 cgtgcataag gatacacttt tttcacatta cgtaccaatt tttcccattg acgctgatcc
     1561 ttcttgtttt tgaatacggg taaaccatag attgtaacag ggttgaggtt tatcaaaggg
     1621 atagtatcat tctcgaatat agtagccctg accactattt gaggtgaagt ttgagcttca
     1681 atggtcattg ctgtacatag aagaagaatg cctgttacaa tcgacttttt cacggtaaaa
     1741 attctcgaaa cgaatttcaa tgagagtgca aatttaatac cggaataaac acccggaaac
     1801 atattccctt actctatttc ctctataaat ctcataattt ttttataagg gtcttataac
     1861 ggacaattat gaattttttt tatttaagtt tgcatataga gtaaaaaagt tggaatcaaa
     1921 aaggaataaa ttcataacaa aaccaaatta ttgaaaatga acagagaatt agtagctacg
     1981 ggtggtggcg attgccctgg attgaatgc
//
LOCUS       DAORPD010000285         1994 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_10469_length_1994_cov_2.795771, whole genome shotgun sequence.
ACCESSION   DAORPD010000285 DAORPD010000000
VERSION     DAORPD010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10469_length_1994_cov_2.795771"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1994
                     /locus_tag="PK976_04180"
     CDS             <1..>1994
                     /locus_tag="PK976_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67116.1"
                     /translation="SSQSRSVNYSIPTSSWGFVSNNPNASDNAEVVNYGLPLPVVENP
                     QSNPSDGVLFDNGPFINSPGTGPGGTDESIIVAGTNSYGFNFNNAIPYTVADDFTVTG
                     TVPWAIGSVEFYGYQTGSTTTSTFTGGYIRIWNGDPSVGGSVIWGDLTTNRMTSTSWI
                     NAYRVNVSGGGTTRPIMKVVCSTPGLTLNPGTYWIEFSATGSIASGPWVLPVCLSTPA
                     SGNALQFTGTWGPIVSGTAPNTYPQGVPFKLNGAALTGLEYQVWRLKQGDETNPATWT
                     SLGTTATTSIADNSWPTLPNGPYRWAVKAKYTGDRWSDAAFSNVLGKGWTSNVTFNIT
                     LSSSTATPQNVMVYMENTSPTADSIYWGLTPASGTINFPHVWKGNYNINIQKFGYQPY
                     TANVDITQNTHTFNILLNETTWAPTDLWVDDRTLIAYWNAPNPQIALFEERWGSGNFT
                     ANQWTVNGTNWSVSSYDGNPAPSAEFNYSPQVTNYEQSIVSKDIQGQGSPGLYLKYDI
                     YLNNYSTTNENQMAVEIWDGTAWNRLKNYTNILGSIPWTSESINISSYTWDTFKIRFL
                     AYGVDSWDINYWNIDNIIVAAALSGKSVLGFDMYLDDIQIGFTTDTTYQIPHNLCLYG
                     HSYTASVDAAYESGASDRDYYTFAAHYLPAPRNLEATPIQ"
BASE COUNT          552 a          505 c          419 g          518 t
ORIGIN      
        1 gtcttctcaa tctagatctg tcaattattc catccctact tcatcatggg gattcgtttc
       61 gaataaccct aatgccagcg ataatgccga agtagtaaat tacggtcttc ctctgcctgt
      121 tgttgaaaat cctcaatcga atccatcgga tggagttctt ttcgataatg gaccctttat
      181 caatagccca ggaacggggc ctggaggaac cgacgaaagt atcatcgtag ccggtaccaa
      241 ttcctatgga ttcaacttta ataatgcaat tccatatacc gttgccgacg atttcaccgt
      301 tactggaacc gttccatggg ctattggttc tgtagaattc tacggttatc aaacaggctc
      361 aaccactact tctaccttta cagggggcta tattagaatt tggaatggag atccaagtgt
      421 aggtggtagc gtaatttggg gtgatctaac cactaatcgc atgacgtcta cttcttggat
      481 aaacgcttac agagtgaacg tgagtggtgg aggcacaacc cgtcccatca tgaaagtagt
      541 ttgctctacc ccaggattaa ccctaaatcc gggaacctat tggattgaat tttcagctac
      601 ggggtctatt gcatccggac catgggttct acccgtatgc ttgagtactc ctgcctcggg
      661 aaatgccttg caatttactg gcacgtgggg ccccatagta agtggaaccg ccccaaatac
      721 ttatcctcag ggtgtcccat tcaaattgaa tggagctgct cttactgggc tcgaatacca
      781 agtttggaga ctcaaacaag gcgatgaaac caatccggct acatggactt ccctgggaac
      841 cacggcaaca accagtattg ccgataatag ctggcctaca ttacctaatg gcccctatcg
      901 ctgggcagtc aaagccaaat atacaggcga ccgctggtcg gatgccgctt tctccaatgt
      961 tctgggaaaa ggctggacat ccaatgtaac cttcaacatt acgcttagct catccaccgc
     1021 taccccgcaa aatgtcatgg tttacatgga aaatacttca cctacggccg actccattta
     1081 ctggggactt acccctgcat ccggaactat caatttccca cacgtttgga aaggaaatta
     1141 caacatcaat atccagaaat tcggctatca accctatacc gctaatgtgg atatcaccca
     1201 aaatacccat accttcaata tactgctcaa cgaaacaaca tgggcaccaa ctgatctatg
     1261 ggtggacgac agaaccctca tagcctattg gaacgcacct aatcctcaga ttgccctctt
     1321 cgaagaacgc tggggatcgg gtaacttcac tgctaaccaa tggacagtga atggaacgaa
     1381 ctggagcgta agctcctacg acgggaatcc agctccttct gctgaattca attattcacc
     1441 ccaggttacc aattacgaac aatccattgt atcgaaggat atccaaggac agggttcacc
     1501 cggtttgtac cttaaatatg atatctatct caacaactat tcaacgacca acgaaaacca
     1561 gatggcggtc gaaatatggg acggcacagc ctggaaccgc ttgaagaatt ataccaatat
     1621 actcggaagt attccatgga catcggaaag catcaatatc tcatcctata catgggatac
     1681 cttcaagatt cgtttcctgg cctacggcgt tgatagttgg gatatcaact actggaacat
     1741 cgataatatt atcgtagcgg cagccctgag tgggaaaagt gttctgggat tcgatatgta
     1801 tctggatgat attcaaattg ggtttaccac cgataccacc tatcagattc ctcataacct
     1861 ttgcctttat ggccattcct atactgccag tgtagatgct gcatacgaaa gtggggcatc
     1921 cgacagggat tattatactt tcgcggcgca ttacctgcct gctcctcgca acctcgaagc
     1981 tactccgatt cagg
//
LOCUS       DAORPD010000286         1994 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_10472_length_1994_cov_2.222795, whole genome shotgun sequence.
ACCESSION   DAORPD010000286 DAORPD010000000
VERSION     DAORPD010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10472_length_1994_cov_2.222795"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..874
                     /locus_tag="PK976_04185"
     CDS             119..874
                     /locus_tag="PK976_04185"
                     /inference="COORDINATES: protein motif:HMM:NF015962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HRC67117.1"
                     /translation="MSGKEQKLELAFTPLLFRHDALSAPYVVVLIDILRATTSICTAF
                     AHGVEAMIPVASLEEARHYKEWGYIIAAEREGIKPDFADLGNSAFHFMKPKLRGKTIV
                     YSTTNGTQAFSVVGYTIDVVIGAFVNLSTLTKWLIEDGRNIYLLCAGWKGNFNLEDTV
                     FAGALAASLMQSSQIIIQSDAVMASIDLWNLAKNNLAEYLLKTEHSQRLKRLGLDDVI
                     PYSLTFDTCPVVPMARNGMIRLATQTNHEHFYD"
     gene            898..>1994
                     /locus_tag="PK976_04190"
     CDS             898..>1994
                     /locus_tag="PK976_04190"
                     /inference="COORDINATES: protein motif:HMM:NF015530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HRC67118.1"
                     /translation="MNKISYTLLLAFVMITACNQTQKKQPNEEFRWQIDRFADIKIMR
                     YQVPGWDSLSLQQKELIYDLSQAALCGRDIIFDQNYKYNLAIRRTLESIYENYRGERS
                     GADWDNFMVYLKRVWFSNGIHHHYSMDKFLPQFSKEYFLQLVADCPDNTLPLQVNQSK
                     DEFLGWISDIIFNPEIAPKRVCLDASKDLVTSSACNFYEGVTQKEVEEFYNSFKIPDP
                     QRPLSLGLNSKVVKKKGKIVEIPYVADGLYGPAIQKIIYWLEKAVNVAENENQKKGLL
                     MLIDYYRTGDLYTWDQYNILWAQDTSFVDYVNGFIEVYGDPLGRKATWESVVDFRNDE
                     ATRRTKIISQNAQWFEDHSPIDPRFKKPKVKG"
BASE COUNT          598 a          402 c          421 g          573 t
ORIGIN      
        1 ctcatgcaat taacaaatta attgaatatg aatgaataaa gtaagctcag gaagaaggtg
       61 aagcttttac ttccgaggtg ttttgtaatt tagccaggcc aaataatttg agagatagat
      121 gtccggaaaa gaacaaaaac tcgaattggc atttacgcct ttactatttc ggcatgatgc
      181 attgtctgca ccctatgtgg ttgtgttgat agatattctg agggctacaa cttctatttg
      241 tacggccttt gctcatggag tggaagccat gattcccgtg gcaagcctcg aagaagcaag
      301 acattataag gaatggggat acattatagc cgctgagcgt gaaggaatta aacccgattt
      361 tgccgaccta ggtaactcag cctttcactt tatgaaaccc aaattaagag gaaaaaccat
      421 agtgtatagc actacaaacg gaactcaggc attttctgtt gttggttata ctatcgatgt
      481 agtaataggt gcttttgtga atttaagcac tctaaccaaa tggctcatcg aggatggcag
      541 gaatatttac cttttatgtg caggttggaa aggcaatttc aatttggagg acactgtgtt
      601 tgctggagca ctggctgcat ctcttatgca atcgagtcaa ataattatcc aaagcgatgc
      661 cgttatggct tctattgact tatggaacct ggctaaaaac aaccttgccg aatatcttct
      721 caagaccgaa catagccaaa ggctaaaacg actggggctc gacgatgtta ttccctactc
      781 tcttaccttc gatacctgcc ctgtagtacc catggctcga aacggcatga ttcgtcttgc
      841 cacgcaaaca aatcatgaac atttttacga ttaaaaccgt ttttaaatct tcatattatg
      901 aataagattt cttacacttt attacttgcc ttcgttatga tcacagcatg caaccaaacc
      961 caaaagaaac aaccaaacga ggaatttcgt tggcaaatcg atcgctttgc cgacatcaaa
     1021 attatgcgtt atcaagttcc aggctgggat agtttaagtc ttcagcaaaa agagctcatt
     1081 tatgatttga gtcaggcagc actttgcgga cgcgatatca tttttgacca gaattataaa
     1141 tataaccttg ctattcgacg cacgctcgaa tccatctacg aaaactatag aggcgagcgc
     1201 agtggagccg actgggacaa tttcatggtt tacttgaaaa gggtgtggtt ttcaaatggt
     1261 atccatcatc attattctat ggataagttc ttaccccagt tcagtaagga atattttctg
     1321 cagttagtgg ctgattgccc cgataatact ctccctcttc aagtgaatca atcgaaggat
     1381 gagtttttgg gctggatttc tgatatcatc tttaatcctg agattgcccc aaaacgtgtt
     1441 tgtcttgatg cttcgaaaga tttagtaacc tcttcggcct gcaattttta tgaaggagtt
     1501 actcaaaaag aagtagaaga attttacaat agttttaaga ttcctgaccc acaacggcct
     1561 ctttcgctcg gattgaattc gaaagtcgtg aaaaagaaag gtaaaatcgt tgaaattcct
     1621 tatgtcgccg atggacttta tgggcctgcc attcaaaaga tcatttactg gctcgagaag
     1681 gcggtaaatg tggcggaaaa cgaaaaccag aaaaaaggtc tactcatgct catcgattat
     1741 taccgaacgg gcgatttata tacatgggat cagtacaaca ttctttgggc acaggacact
     1801 tccttcgtag attacgtaaa tggtttcatc gaagtttatg gcgatcctct gggacgcaag
     1861 gccacatggg aatcggtagt agattttcgg aatgatgagg ccacacgccg taccaagatc
     1921 atcagccaaa atgcacagtg gttcgaagat cattccccca tcgatcctcg tttcaagaaa
     1981 ccaaaggtta aggg
//
LOCUS       DAORPD010000287         1994 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_10473_length_1994_cov_2.184116, whole genome shotgun sequence.
ACCESSION   DAORPD010000287 DAORPD010000000
VERSION     DAORPD010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10473_length_1994_cov_2.184116"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="PK976_04195"
     CDS             <1..395
                     /locus_tag="PK976_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67119.1"
                     /translation="IEGELSDNPPSLISTGLGGKDNKLRMFIAIFPSFEGTFSENQAR
                     IIRMEWEETCKNYQIETEKLRFLFEYTSLRILVPLNIDLSAVVKEFIAECNKYGNFVY
                     EGFLATNVAIPSHREILSAIERIKNHPY"
     gene            467..>1994
                     /locus_tag="PK976_04200"
     CDS             467..>1994
                     /locus_tag="PK976_04200"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HRC67120.1"
                     /translation="MKTKLTIILSLVLGLGILNSGKAQQVFERGSYRCHLDKIKKYST
                     EYFRNEEKNYFPKSFDVLKYQLDVDIWSCYQSPYSHAFDGKLVITLKADSLLDHVKLN
                     AVNSSLQIQEVSIAASTFTHSGNILTINLDTTMIPGDTFQIAIKYHHNNVNDYAFYAK
                     NGFVFTDCEPEGARCWFPCYDRTSDKAQWELTAKVPSNVKLGSNGILQDSVKIADTIY
                     YTWKSDENVATYLIALTSRTNYNLKIINYTNPNDTSEHFPFRFYYNQGENPQNIINVI
                     DDVTTYFSSTFCKHPFPKNGFASLNDEFQWGGMENQTLTSICPNCWDESLIVHEFSHQ
                     WFGDMITCATWADIWLNEGFATFSESLWLGEKSGYNAYKQDVLQNASYYLSANPHWPI
                     SDPSWAITTPSSDILFNYAITYCKGACVLHQLRYTLGDSIFFNIIHQYAHDSSFRYQS
                     ATIPDFINIVNSVTGEDYQWFFDEWIYMPNHPIYQNYYSITQIDDQHWGLYLEVNQTQ
                     T"
BASE COUNT          605 a          436 c          350 g          603 t
ORIGIN      
        1 taatcgaagg agagttgagc gacaatcctc ccagccttat ttcgacgggt ttgggcggta
       61 aagacaacaa actgcgcatg tttattgcca tcttcccttc ctttgaagga actttcagcg
      121 aaaaccaggc tcgcatcatc cgtatggaat gggaagaaac atgtaaaaac tatcagattg
      181 aaaccgagaa acttcgtttc ttgttcgaat acacctctct tcgtattctt gtacctctga
      241 atatagacct gagtgcagtg gtgaaggaat ttatcgccga atgcaataaa tatggcaatt
      301 tcgtttatga aggatttctc gctaccaatg tggctattcc ctctcaccgt gaaattttat
      361 cggccatcga aagaataaag aatcatccat attaatcttt tctctatttt ataaaattgg
      421 ttcttcgaaa aaaatcctat cttcgcccat tcaaagcata accattatga aaaccaaact
      481 taccataatt ctttccctcg ttttgggcct tggaatatta aattctggaa aagctcaaca
      541 agttttcgaa agagggtcgt atcgctgtca tctcgataaa ataaaaaaat attctactga
      601 atactttcgc aacgaagaga aaaattattt ccccaagtcg ttcgatgttt tgaaatatca
      661 actcgatgtt gatatctggt cgtgctacca atcgccttac tcgcatgctt tcgacggtaa
      721 gctggtcatt accttgaaag ccgacagttt gctcgatcat gtaaagctga atgcagtgaa
      781 ttcatctctt caaattcagg aagttagtat tgctgcctcc accttcaccc acagcggtaa
      841 catccttacc atcaatctcg atactactat gataccgggc gacacctttc agattgccat
      901 aaaatatcat cataacaatg taaatgacta cgccttctat gctaagaatg gctttgtatt
      961 taccgattgt gaacccgaag gtgcccgctg ctggtttccc tgttacgacc ggacatctga
     1021 taaggcccaa tgggaattaa ccgctaaagt accttccaat gtaaaacttg gaagtaatgg
     1081 tatattgcaa gattcggtca aaattgccga tactatctat tatacttgga aaagtgatga
     1141 aaacgtggct acgtatctca ttgccctcac ttcgagaacc aattacaacc tcaagatcat
     1201 aaattatacg aatcctaacg acacatccga gcatttcccc ttcaggttct attacaatca
     1261 aggcgaaaat cctcaaaata tcatcaatgt tatagatgat gtaacaactt attttagttc
     1321 aactttttgc aagcatccct ttcccaagaa tggatttgca agcctgaacg acgaatttca
     1381 atggggtgga atggagaacc agactcttac gagcatttgt cccaattgct gggacgaaag
     1441 cctcattgta catgaatttt cacatcagtg gtttggcgat atgataactt gtgcaacctg
     1501 ggccgatatc tggcttaacg aaggatttgc cacctttagc gaaagtctct ggctgggaga
     1561 aaaatcgggt tataatgctt acaaacagga tgtgcttcaa aatgcttcct attatctatc
     1621 agctaaccca cattggccta tttctgatcc aagttgggct attactactc cctcctctga
     1681 tatattgttc aactatgcca taacttactg taaaggcgct tgtgtacttc atcagctaag
     1741 atatactttg ggcgattcaa tatttttcaa tattattcac caatatgccc atgattcatc
     1801 gtttcgatac caatcagcta ctatccctga cttcatcaat attgtaaact cagttacggg
     1861 tgaagattat caatggtttt tcgatgaatg gatatatatg cccaatcatc ctatttacca
     1921 aaattattac agcatcacgc aaattgacga tcaacattgg ggattatatt tagaagttaa
     1981 tcaaactcaa acca
//
LOCUS       DAORPD010000288         1993 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_10477_length_1993_cov_2.523736, whole genome shotgun sequence.
ACCESSION   DAORPD010000288 DAORPD010000000
VERSION     DAORPD010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10477_length_1993_cov_2.523736"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="PK976_04205"
     CDS             <1..233
                     /locus_tag="PK976_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67121.1"
                     /translation="KNDSGSAKHFATKVKRNSKAFVLSHKKLYFSLLLSFFIKAKVEE
                     DAFDRAVDKGSYFIGRERKKSEKIILLQRKDD"
     gene            196..1584
                     /locus_tag="PK976_04210"
     CDS             196..1584
                     /locus_tag="PK976_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67122.1"
                     /translation="MKKSYYFSEKTIEAIKSSEVQFLLRFGFGIIKGDILHAAPYGVW
                     SFHHDDPEVIRGVPSNFWEILEDIPVNGAILQQLDESIDCGKILWQGWFPTINHSWKA
                     NLDQAYYGSVEWPAQTCREILFKGADCLKNKPFPKPAPMRFAPINCIMLKFFLKLWAN
                     KVKFHFNELFRAEHWSIGFIESSIKNFIGNNSQPENIKWIRSGSRRQYYADPAGIYQN
                     HQLKILCEHYDYKPAHGEICLFKTNHHSTIYRPEKVLLNASWHLAFPFIFEAEGNIWC
                     LPESAQSNQLRLYRWDTKKEELMFEKIIISDLQAVDPIIFHHNRKWWLMFTDKTKSNY
                     SLHLWYAEDFRGPYFPHINNPVKTDIRSSRPAGNIFYMDGRLMRPAQDCSVISGHRIT
                     LNEIIEITPFSFVETTRFFVEPPKSRQHFKGMHTLNTAGPYVIFDVKQDLFIWDNFKR
                     RLLRKTKKIIVQ"
     gene            complement(1787..>1993)
                     /locus_tag="PK976_04215"
     CDS             complement(1787..>1993)
                     /locus_tag="PK976_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67123.1"
                     /translation="NRVLKPQGYFMMSSDYLYLSWSVEDYMRHSRLHLVKLAMENGYT
                     VYFIESFGGILTSIYSMVIRYMRI"
BASE COUNT          678 a          371 c          367 g          577 t
ORIGIN      
        1 taaaaaatga ctctggaagt gcaaaacatt ttgccaccaa agtcaaaagg aattcgaagg
       61 cttttgtgct atcccataaa aaactttatt tttctctttt attatcgttt tttattaaag
      121 ccaaagtcga agaggatgct ttcgatagag ccgtggataa aggaagctat tttattggaa
      181 gagaacgcaa aaaaagtgaa aaaatcatat tacttcagcg aaaagacgat tgaagctatc
      241 aagagttcgg aagttcaatt ccttctgaga tttggtttcg gaatcatcaa aggcgatatc
      301 cttcatgcgg ctccttatgg agtttggtcg tttcatcatg acgatcccga agtcattaga
      361 ggagtgccat caaatttctg ggaaatctta gaagatatcc cggtgaatgg tgctatatta
      421 caacagctcg acgaaagtat agattgcgga aaaattctat ggcaaggatg gtttcccacc
      481 atcaatcatt cttggaaagc caacctcgat caagcatact atggttcggt agaatggccc
      541 gctcaaacgt gtagagaaat tctattcaaa ggtgcagact gcctaaaaaa caaacctttc
      601 ccaaaacctg cacccatgcg attcgctcca atcaattgta taatgttgaa attctttctc
      661 aaactatggg cgaacaaggt aaaatttcat ttcaatgaat tatttcgtgc tgaacactgg
      721 agcattggat ttatagaaag ttcgataaaa aattttatag gaaacaattc tcaacctgaa
      781 aatatcaaat ggatacgtag tgggtcaaga agacaatatt atgctgatcc agcagggatt
      841 taccagaatc atcaactgaa aattctatgt gaacattacg attacaaacc agctcatggc
      901 gagatatgtc ttttcaaaac aaatcatcat tcaactattt atagaccaga aaaagttctt
      961 ctgaatgcct cctggcatct tgcctttcct tttatttttg aagcagaagg gaatatctgg
     1021 tgcttacccg agtcggccca aagcaatcag ctcaggttat acagatggga cacgaaaaag
     1081 gaagaactta tgttcgaaaa aattattatt tccgatttac aagcggtcga tcccatcatt
     1141 tttcatcata atcgaaagtg gtggcttatg tttaccgata aaaccaaaag taattacagt
     1201 ctgcacttat ggtatgccga agattttaga ggcccttatt ttccacacat caacaatcca
     1261 gtaaaaactg acattcgttc atcgagaccc gcaggcaata tattttacat ggatggccga
     1321 ctaatgcgcc cggctcagga ttgctccgtc atatcaggac atagaatcac ccttaatgaa
     1381 ataattgaaa taactccttt ttctttcgtc gaaaccacca gatttttcgt agaacctcca
     1441 aaatcgaggc agcatttcaa agggatgcac actttaaata cagctggacc ttatgtgatc
     1501 ttcgatgtta aacaagatct ttttatctgg gacaatttca aaagaaggct tttgagaaaa
     1561 acaaagaaaa taattgttca ataaaatgaa aagacaaggc taagtgtttt tctcacaact
     1621 atcagaatat tcatggtata gctaaattct ttgattttat tgcattcgaa aaatagataa
     1681 tcgacctact aataatgaaa gcggagaaat aacaataaaa gaataaaagc catagaattc
     1741 tctataattt catgacagga tgaattgctt ggtggattct ttcgggttag attctcatat
     1801 agcggattac catagaatag atgctggtga ggattccacc aaaactttcg atgaaataaa
     1861 ctgtatatcc attttccata gccaatttaa cgagatgcag acgtgagtga cgcatatagt
     1921 cttccacgct ccatgaaaga taaagataat cggatgacat catgaaatat ccttgcggtt
     1981 tgagtactct att
//
LOCUS       DAORPD010000289         1993 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_10478_length_1993_cov_2.137255, whole genome shotgun sequence.
ACCESSION   DAORPD010000289 DAORPD010000000
VERSION     DAORPD010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10478_length_1993_cov_2.137255"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /gene="rpsE"
                     /locus_tag="PK976_04220"
     CDS             <1..444
                     /gene="rpsE"
                     /locus_tag="PK976_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HRC67124.1"
                     /translation="TTKVTKGGRTFTFAAMVVVGNEDGIVGWGLGKAGEVTTAIAKGV
                     EAAKKNLVKVPVYKGTIPHEQTAHYGGAEVFLKPAFTGTGVKAGGAMRAVLESVGITD
                     VLAKSKGSSNPHNLVKATILALSEMRDPITVAQNRGVSLDKVFNG"
     gene            465..641
                     /gene="rpmD"
                     /locus_tag="PK976_04225"
     CDS             465..641
                     /gene="rpmD"
                     /locus_tag="PK976_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004331515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HRC67125.1"
                     /translation="MATIKIKQTRSRIDCPRVQRRTLDALGLTKIGRVVEHEDTPAIR
                     GLIAKVSHLVTVID"
     gene            672..1121
                     /gene="rplO"
                     /locus_tag="PK976_04230"
     CDS             672..1121
                     /gene="rplO"
                     /locus_tag="PK976_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HRC67126.1"
                     /translation="MNLSNLKPAEGSTKTRKRIGRGPGSGKGGTSTRGHKGAKSRSGY
                     KHKIGFEGGQMPLQRRLPKFGFNPLKRTEYRAINLDKLQEIADSQNVSKIDIETLINA
                     GLISPKHSVKILGKGSLTAKLEVTAHAFSKSAEAAIIAAGGNVVKIQ"
     gene            1123..>1993
                     /locus_tag="PK976_04235"
     CDS             1123..>1993
                     /locus_tag="PK976_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA];
                     GO_process: GO:0065002 - intracellular protein
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HRC67127.1"
                     /translation="MRFIEILKNIWKIEDLRHRLIITASLVAVYRFGSFVVLPGIDPA
                     LLTVLQNNASSGLLGLLDMFSGGAFSNASIFALGIMPYISASIVMQLLGIAVPYFQKL
                     QREGESGHTKMNQYTRYLTVAILVLQGPAYIYGALQGAIPGGFWDWVLPSTIILAAGS
                     MFVLWLGERITDKGVGNGVSFIILIGIIARLPHALVAEVDRLLNAPGGLLVLLLEILF
                     LMVVTAFAIMLVQGTRKVPVQYAKRIVGNKQYGGVRQYIPLKVNAANVMPIIFAQAIM
                     FIPITIAQFTAQGG"
BASE COUNT          576 a          381 c          481 g          555 t
ORIGIN      
        1 acgacgaaag ttaccaaagg aggacgtaca tttacctttg ccgctatggt tgtagtgggt
       61 aatgaagatg gtatcgtagg ttggggactt ggtaaagcag gtgaagtaac aacggctatt
      121 gccaaaggag tagaagctgc aaagaagaat ctggtaaaag tgcccgtgta taagggaaca
      181 attcctcacg aacaaaccgc acattatgga ggtgcagaag tgtttctgaa accggcattc
      241 acaggaaccg gtgtaaaagc aggaggtgct atgcgtgctg ttctcgaaag cgtagggatc
      301 accgatgtat tggcaaaatc aaaaggttcg tccaatcctc ataatcttgt gaaagctacc
      361 attcttgcgc tgagtgaaat gagagatccc ataactgttg ctcaaaacag aggcgtgagt
      421 ttggataaag ttttcaatgg ttaaaaattt ggaggaacac taatatggca actatcaaga
      481 tcaaacaaac aagaagccga atcgactgcc caagagttca gagaaggacg ttggatgctc
      541 tcggacttac taaaatcggg cgtgttgttg aacacgaaga tactccggct atacgtggct
      601 taattgcaaa agtaagccat ctggttactg tgatcgatta aattagttta taacgataaa
      661 aatacacaat aatgaattta tcgaatttaa aaccggcaga aggatcaacc aaaactcgga
      721 aaagaatagg tcgaggaccc ggatcgggta aaggtggaac ttctaccaga ggacataaag
      781 gagctaaatc acgttcgggt tacaaacaca aaatcggatt cgaaggtggg caaatgcctt
      841 tacagcgccg actgccaaag ttcgggttta atcctttgaa aaggacggaa tacagagcta
      901 ttaatttgga caaacttcaa gagattgccg attcgcaaaa tgtatcgaaa atagatatcg
      961 agacattgat aaatgctggt ctgatttctc cgaaacattc ggtgaaaatc ctcggcaaag
     1021 gctcgttaac tgccaaattg gaagtcacag ctcatgcctt ttctaaatcg gctgaagcgg
     1081 ctattattgc tgcaggtgga aatgttgtaa aaatccaata agatgagatt tatcgaaatt
     1141 ctaaaaaata tttggaaaat tgaagacctt cgtcaccggc ttatcattac ggcttcctta
     1201 gttgcagtgt acaggttcgg ttcctttgtg gttcttcccg gaattgatcc tgctttgttg
     1261 actgttctgc aaaacaatgc aagctcgggg cttctgggtt tgttggatat gttctcggga
     1321 ggtgcttttt caaatgcctc tatatttgca ttgggaatca tgccatacat ttcggcctct
     1381 atcgttatgc aattgttggg tatcgcagtt ccttattttc aaaaattgca gcgtgaaggc
     1441 gaaagcggac atacaaaaat gaatcaatac acacgttatt tgactgttgc gattcttgta
     1501 ctgcaagggc ctgcatatat ctacggggca ttacagggag ctatacccgg cgggttttgg
     1561 gattgggttc ttccctctac cattatttta gccgccggaa gtatgttcgt attgtggttg
     1621 ggtgaaagaa ttaccgacaa aggcgttgga aacggagtct cgtttatcat tcttataggt
     1681 atcattgctc gtttgcctca tgctttggtt gccgaagtcg atcgattgct caatgcacct
     1741 ggaggtttgc tcgtgctgct actcgagatt ctcttcctga tggtagttac ggcatttgct
     1801 atcatgcttg ttcaaggaac tcgaaaagtt cctgtgcagt atgccaaacg tatcgttggt
     1861 aacaaacaat atggcggagt acgtcagtat attcctctta aagtgaatgc tgccaatgtg
     1921 atgcctatca tctttgctca ggcaatcatg tttatcccca tcacgattgc tcagtttacg
     1981 gcgcagggtg gtg
//
LOCUS       DAORPD010000290         1992 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_10489_length_1992_cov_2.179659, whole genome shotgun sequence.
ACCESSION   DAORPD010000290 DAORPD010000000
VERSION     DAORPD010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10489_length_1992_cov_2.179659"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1642)
                     /gene="thrS"
                     /locus_tag="PK976_04240"
     CDS             complement(<1..1642)
                     /gene="thrS"
                     /locus_tag="PK976_04240"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HRC67128.1"
                     /translation="MINIKFPDQSIRQYPEGVTGLEIARSINEGLARKVLAIKLNGEV
                     WDVTRPIFQDASIRLLTWDDDEGKATMWHSSAHLMAEAIESLYPGVKFGVGPDIENGF
                     YYDIDFGDYQISSDDFKKIEDRMLELARQKQEFKRRDVSKAEALEYFRRKGDEYKIEL
                     INDLEDGSITLYETGQFTDLCRGPHLPNTSPIKAVKLLSIAGAYWRGDEKRKMLTRLY
                     GITFPKAEMLAEFLNMLEEAKKRDHRKIGKELDLFHFSSRVGNGLPLWTPRGALLRER
                     LTDFLKKELYKRGYVLVASPHIGNVNLYKTSGHYEKYGADSFRPISTPEPGEEFMLKP
                     MNCPHHCEIYNRKPMSYKELPQRYAEFGTVYRYEQSGVLHGLTRVRGFTQDDAHIFCR
                     PDQLKEEFIGVIDLVLFLFKTFDFNNFIAQVSLRDPENKIKYIGSDENWDRAEQAIIE
                     AAEEKNLNPVLVKGEAAFYGPKLDFIVKDAIGRKWQLSTIQVDYNLPERFELEYIGAD
                     NQRHRPIMIHRAIFGSIERFVAVMLEHTAGKFPLWLAPT"
BASE COUNT          569 a          417 c          378 g          628 t
ORIGIN      
        1 gtgtgggagc tagccataaa ggaaatttcc cggctgtgtg ctccagcatc acggctacaa
       61 aacgttcgat cgaaccaaaa atggctctat gaatcataat agggcgatgc cgttggttat
      121 cggcaccaat atattccaat tcgaaacgtt cgggtaagtt ataatccact tgtatcgtac
      181 taagctgcca ttttcgccca atggcatcct ttacaataaa atcgagtttt gggccgtaaa
      241 aagctgcctc accttttacc aagacagggt tcaaattttt ctcttcggcg gcttctatga
      301 tggcttgttc ggctctatcc cagttttcat ccgatccaat gtacttaatc ttattctcag
      361 ggtctctcaa cgaaacctgc gcaatgaagt tattgaaatc gaaagttttg aaaagaaaaa
      421 gaacaagatc gatcaccccg ataaattctt ctttcaattg gtcgggacgg caaaaaatat
      481 gtgcatcatc ctgagtaaag cctcttactc tggttaatcc atgcaaaaca cctgactgtt
      541 cgtaacgata aaccgttcca aactcggcat atctttgagg caattcttta taagacattg
      601 gtttacgatt gtagatttcg caatgatgtg gacagttcat gggtttcagc ataaactctt
      661 caccaggttc gggagtagaa atgggtcgga aggaatcggc accgtatttt tcataatggc
      721 ctgaagtttt ataaaggttg acattgccaa tgtgaggtga agccactaag acataacctc
      781 ttttatagag ttcctttttc agaaaatcgg tgagtcgttc gcgcagtaaa gctccgcgcg
      841 gcgtccaaag gggcaaacca ttacccacgc gtgaagagaa atgaaataaa tcgagctctt
      901 ttccaatttt acgatgatct cttttttttg cttcttcgag catattgagg aattcagcaa
      961 gcatctcggc tttcggaaag gtaatgccat agagacgcgt aagcattttg cgtttttcat
     1021 ctcctctcca gtaagcaccg gctatcgata ataattttac cgccttgatg ggcgaggtat
     1081 tgggcaaatg tggcccacgg cacaaatcag taaattgacc tgtttcgtat aaagtgatgc
     1141 taccatcttc caaatcattg attagttcga tcttatactc atcgcctttg cgtcgaaaat
     1201 attccagcgc ctcggcttta ctcacatctc ttcttttgaa ttcctgcttt tgacgtgcaa
     1261 gttctagcat gcgatcttcg attttcttga agtcgtctga tgaaatctgg taatcaccaa
     1321 aatcgatatc ataatagaaa ccattctcta tatcgggacc aactccgaat tttacaccag
     1381 gataaaggct ttcgatggct tctgccatta aatgcgccga tgaatgccac attgtggctt
     1441 tgccttcatc atcatcccat gtgagtaaac gaatgctggc atcttgaaat ataggccgcg
     1501 taacatccca aacttcaccg tttaacttga tggcaagcac cttccgcgct aaaccttcat
     1561 ttatgcttct ggctatttct aatcctgtaa ctccttctgg atattgccga atactttgat
     1621 cgggaaattt tatgttaatc attatcaaat aattgtgata aaacaaaact gatctcattg
     1681 ggtcaatttt gggcgtgcaa agttaggttt tctcatctga tctttaaaat catttcatgt
     1741 cgataattta aagtgtatca atgtcgaaca taaaggataa gaaaagatgt attttactcc
     1801 attttcaaag agtaaatcct ttcatttttt tcttaaataa tgagtagctc actagatccg
     1861 tataaagaga tataggtctg gcgaaccata gtagaacatt aatctgcaat gttttaaatt
     1921 ttaatttttc tttctgagcc ataactctat gattttgtca aaagagcatt atctttgtaa
     1981 aaaatgtttc ag
//
LOCUS       DAORPD010000291         1988 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_10513_length_1988_cov_2.045525, whole genome shotgun sequence.
ACCESSION   DAORPD010000291 DAORPD010000000
VERSION     DAORPD010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10513_length_1988_cov_2.045525"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(199..456)
                     /locus_tag="PK976_04245"
     CDS             complement(199..456)
                     /locus_tag="PK976_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HRC67129.1"
                     /translation="MILFVIFLIWLTFFDPNSLITQIKLSHQEKQMRQEAEWYRKEIN
                     NYKTQLNEILYNPEVTEKFVREQYLMKRPNEEIFLIARPVE"
     gene            complement(498..1064)
                     /locus_tag="PK976_04250"
     CDS             complement(498..1064)
                     /locus_tag="PK976_04250"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HRC67130.1"
                     /translation="MILTKEEALKLLNEHVQNPRMIQHCLSSEAVMRALARRLGKDED
                     RWGLAGLLHDIDVEYTNADPLTHGLIGAQWLEDMGIDKDIVDAIRMHNEMAAQKPRTT
                     VFEHALSAGETITGLINATALVYPDKDIQLVKPKSIIKRMKEKAFAASVKRENIMECE
                     DIGLSLEEFITIALEAMQAIPDYNEFNK"
     gene            complement(1024..1581)
                     /locus_tag="PK976_04255"
     CDS             complement(1024..1581)
                     /locus_tag="PK976_04255"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRC67131.1"
                     /translation="MESTNTNIKPFIHLREASKDDAPIILELICELADYERAPEQVTL
                     TEAELLNDGFGERPLFKVILAETGGKTVGMALWYFAYSTWKGKVLYLEDIIIKKDFRG
                     MGIGSLLFEELILQAGKHRAKRMQWQVLNWNEKAIRFYQKYGANMSSEWLNGSLTEEQ
                     ILNLKDKIQPFHHDINEGRSFETFK"
     gene            complement(1556..>1988)
                     /locus_tag="PK976_04260"
     CDS             complement(1556..>1988)
                     /locus_tag="PK976_04260"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HRC67132.1"
                     /translation="GPEIIKMLGDFFYKQNWKFTLHSQILPDNAECLKNIITQSFNEK
                     TDLLITTGGTGIGPHDTTVDVVAPFLQKQIPGIMEIIRIKYGTVNPAALLSRSVAGIY
                     DKTLVFTLPGSTKAVREYLNEIFKILPHCFEMLYGIDKHKH"
BASE COUNT          527 a          373 c          402 g          686 t
ORIGIN      
        1 gaaagtaatt tagataaaca ttagaaaatt tcgaaatcat tgaagaaggg atattgcttt
       61 tttatccctt cttttttggt atattacagg aaagatgaag gtttgtaata aaaattataa
      121 atcagaatag gttgaaaaca aaaaaatctg atttattttg ataatttttg ttaaagattt
      181 tgtatccttc cattttattt attcaacagg acgagctata agaaaaattt cttcgttggg
      241 tcttttcatc aaatactgtt ctcttacgaa tttttcggta acttcgggat tgtacagaat
      301 ttcgttgagc tgggttttat agttgttgat ttcttttcga taccattcgg cttcctggcg
      361 catttgtttt tcctgatgcg ataatttgat ttgtgtaatc aggctattgg gatcgaaaaa
      421 ggtgagccag ataagaaata tgacaaacag aatcaagaat aaatacttat gggaaaaatg
      481 ttttaactta ttgactttca tttgttaaat tcattataat cgggtatagc ttgcatggct
      541 tcgagggcaa tagtaataaa ttcttcgagg ctaaggccta tgtcttcgca ttccataata
      601 ttttctcgtt ttaccgatgc agcgaaggct ttttccttca ttcttttgat aatggattta
      661 ggttttacta attgaatgtc tttatcggga tagaccagtg cggtagcgtt tatcagtccg
      721 gtaatggttt caccggctga cagcgcatgt tcgaagacag tagtccgagg tttttgagca
      781 gccatttcat tgtgcattcg tatggcatcc actatatcct tgtcgatgcc catatcttcg
      841 agccattggg cacctatgag gccgtgggtg agggggtcgg cattcgtata ttctacgtct
      901 atatcatgca gtaaacccgc aagcccccat cggtcttcat ctttccctaa ccgacgtgct
      961 aaagccctca tcactgcctc actcgaaaga caatgctgga tcattcgggg attttgtaca
     1021 tgctcattta aaagtttcaa agcttcttcc ttcgttaata tcatgatgaa atggttggat
     1081 tttatctttc aaatttagaa tctgttcttc tgtcagactg ccatttagcc actccgaact
     1141 catatttgcc ccatatttct gatagaatcg tatagctttt tcattccagt tcaaaacctg
     1201 ccattgcatt ctttttgccc gatgttttcc tgcctgcaaa ataagctctt cgaatagcag
     1261 gcttccaatg cccattcctc tgaaatcttt tttaatgata atatcttcga gatacaatac
     1321 tttgcctttc caggtagaat atgcaaaata ccacagtgcc atgcctactg ttttacctcc
     1381 ggtttctgct aaaattacct tgaagagtgg tcgttctcca aatccatcat tgaggagttc
     1441 ggcttcggtt aatgtaacct gttctggagc tcgttcgtaa tcggcaagtt cgcagattag
     1501 ctcgagaatg atcggagcat catctttcga agcttctcta agatgaatga agggtttaat
     1561 gtttgtgttt gtcgattcca tataacattt cgaagcaatg gggtaagatc ttgaatattt
     1621 cattcaggta ttctcgaacg gcttttgtgc tacctggaag agtgaacacg agggttttgt
     1681 cgtaaatccc tgccacggat cgcgagagta aagcggcagg attcacggta ccatatttta
     1741 tccgtattat ttccatgatg ccgggaattt gtttttgaag gaagggggca acaacatcta
     1801 cagtagtatc gtgaggtcca atacccgttc cacctgttgt aatcaggagg tcggtctttt
     1861 cgttaaaaga ttgggttatt atatttttca aacattcggc attgtcgggc aggatttgcg
     1921 aatgaagggt aaatttccaa ttttgtttgt agaaaaaatc gccgagcatt tttatgattt
     1981 ccggcccg
//
LOCUS       DAORPD010000292         1987 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_10522_length_1987_cov_1.931677, whole genome shotgun sequence.
ACCESSION   DAORPD010000292 DAORPD010000000
VERSION     DAORPD010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10522_length_1987_cov_1.931677"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..1161)
                     /locus_tag="PK976_04265"
     CDS             complement(58..1161)
                     /locus_tag="PK976_04265"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HRC67133.1"
                     /translation="MKNLKLKLVVSVILIASVLSGKAAIITPPDEGMWLPMFIERLNY
                     TDMQKLGLRLTPEELYSINHSSLKDAVVGLAAGSQPEGYFCTGEIVSDQGLLFTNHHC
                     GYNYIQEHSTLENDILTNGYWAMSFEEELPNPGLTASLLVRMENVTKAVLQNVTPEMD
                     EDAREQAISEVTDSIVKEASEGKKYNCTVKSFFNGNEYYLFVYQTYEDVRLVGAPPSS
                     VGKFGGDTDNWMWPRHTGDFSIFRIYTAPDGSPAPYSKENVPLKPKHFLPVSIKGVQK
                     GDFAMIWGFPGGTDRYMTSWEVKQAIDQTEPAIVKIRDLKLKIMKEDMQTDPRINLMY
                     AAKYAATSNYWKYYIGQIRGLKRLNVYEKKLKR"
     gene            1284..>1987
                     /locus_tag="PK976_04270"
     CDS             1284..>1987
                     /locus_tag="PK976_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRC67134.1"
                     /translation="MRYQNDTRIVMTLDGGGTNFVFSAMQAEKEIITPFAFPSQASTL
                     SETLYNIETGFRRIKSLLPGEPAAISFSFPGPADYELGIIGDLVNLSVFRGGVPLGPY
                     LEDIFKIPVFINNDGDLFAYGEAISGFLPEVNRWLADAGSPKRFQNLLGVTFGTGFGA
                     GIVLRGELFLGDNSAAGEINRMSNKLYRNMSVEESVSIRGVRRVFSNYSNIPFDQTPS
                     PKEIYEIAIGKRPGNQ"
BASE COUNT          543 a          407 c          406 g          631 t
ORIGIN      
        1 cccctttttc attagctaaa tacatattag aagccttcat acctaccaag gacgtttcta
       61 tctttttaat ttcttttcgt aaacattcaa gcgttttaag cctcgaattt gccctatata
      121 atatttccag taattacttg ttgcagcata tttggccgca tacatgagat taattctggg
      181 atcggtctgc atatcttctt tcatgatttt taattttagg tcacgaattt ttacgatagc
      241 aggttcggtt tggtcaatag cctgttttac ctcccaagaa gtcatataac gatcggtacc
      301 accagggaat ccccagatca ttgcaaaatc acctttttgt acgcctttga tagaaaccgg
      361 cagaaagtgc ttgggtttta gaggaacatt ttctttgctg taaggagccg ggctaccatc
      421 tggagcggta tagattctga aaatactaaa atcgcctgta tgacggggcc acatccaatt
      481 atcggtgtct ccaccaaatt ttcccaccga cgaaggtggt gcacctacca gtcttacgtc
      541 ttcataagtt tgatacacaa ataaataata ctcattccca ttgaaaaacg atttgaccgt
      601 acaattatat tttttccctt cggatgcttc cttgacgatg gagtctgtaa cttctgatat
      661 ggcctgttct ctggcatctt catccatttc gggagtcaca ttttgtaata cggctttagt
      721 aacattttcc atgcgaacaa gcaatgaagc cgtgagtcct ggattgggca gttcttcttc
      781 gaaactcatc gcccagtaac cattcgtcag aatgtcgttt tcaagtgtgc tgtgttcttg
      841 aatgtaattg tatccgcaat gatgattggt aaatagcaaa ccttgatcgg atacgatttc
      901 tcctgtacag aaatatcctt cgggttgtga accggcagct aaaccaacta ctgcgtcctt
      961 caaactcgag tgattgatac tgtatagttc ttcgggtgtc agccttaatc ccagtttttg
     1021 catatcggta tagttgagac gctcgataaa catgggaagc cacatccctt catcgggagg
     1081 tgtgattatg gctgcttttc ctgacaatac actggctatc agtattaccg acacaaccag
     1141 tttaagtttt aaatttttca tatagtttgt tttaagtaat tgaatgagat aacagaaatt
     1201 taccatttac aaaattaaag agaggccaaa attaccaatt ttaagaaata tttgatttct
     1261 ttgcactgcc aaaatctctg acgatgcgat atcagaatga tacacgaata gttatgaccc
     1321 tcgatggggg agggacaaat tttgtattca gtgccatgca ggccgaaaaa gaaattatca
     1381 cgccatttgc ttttccttca caggcttcta ctttatcaga gactttatat aatattgaaa
     1441 cgggttttag aagaataaaa agcttacttc ccggcgagcc cgcagccatt agttttagtt
     1501 ttcccggtcc cgccgattat gaattgggca ttattggcga tctggtcaat ttatcggtct
     1561 ttcgtggagg tgtcccctta gggccttatc tcgaagatat atttaagatt cccgttttta
     1621 tcaacaatga tggggatttg ttcgcctatg gcgaagccat atcaggattt ttgcctgaag
     1681 taaacagatg gcttgccgat gctggcagcc ctaaacgctt tcagaattta ttgggagtta
     1741 ctttcggaac gggtttcggt gcaggaattg ttttaagagg agaattgttt ttgggcgata
     1801 actcggctgc tggtgagatt aaccgaatga gcaataaact ttatcgcaac atgagtgtag
     1861 aggaaagtgt gagcatccgt ggggttagac gcgtattttc caattattcc aatattccat
     1921 tcgaccaaac acctagcccc aaggaaattt atgaaatcgc cattggcaag aggccgggca
     1981 atcagaa
//
LOCUS       DAORPD010000293         1982 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_10555_length_1982_cov_2.301505, whole genome shotgun sequence.
ACCESSION   DAORPD010000293 DAORPD010000000
VERSION     DAORPD010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10555_length_1982_cov_2.301505"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            42..356
                     /gene="rplU"
                     /locus_tag="PK976_04275"
     CDS             42..356
                     /gene="rplU"
                     /locus_tag="PK976_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HRC67135.1"
                     /translation="MYAIVEIAGQQIKVETKREYYVNRLQGDEATQVIFENILLIDDD
                     STIRVGRPRVEGARVKATISEHIKSDKVLIFKKKRRKGYQKLNGHRQPLSKIIIEEIS
                     VA"
     gene            377..646
                     /gene="rpmA"
                     /locus_tag="PK976_04280"
     CDS             377..646
                     /gene="rpmA"
                     /locus_tag="PK976_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HRC67136.1"
                     /translation="MAHKKGAGSSQNGRESESKRLGVKIFGGQFAKAGQIIVRQRGTI
                     HHPGINVGLGKDHTLFALSDGIVEFHRRKNNKSYVSVFPDNKKEN"
     gene            749..>1982
                     /gene="serS"
                     /locus_tag="PK976_04285"
     CDS             749..>1982
                     /gene="serS"
                     /locus_tag="PK976_04285"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HRC67137.1"
                     /translation="MIPLQIIREQPDWVIQRLAVKNFDANSLIYQILELDWKRKSTQF
                     ESDERSAEYRKLTQEIGSLIKAGQAGKANDRKNEVAKIKEEVRNLQERLDEISRQMNE
                     LLVRLPNIPDERVSPGISPADNEIVYSEGSIPQLPPEALCHWDLAVKYQIISFEIGSK
                     VTGAGFPFYKDKGAKLQRSLISFFLDEATKAGYTEYEPPLLVNEDSAFATGQLPDKEG
                     QMYFVPQDNFYLIPTAEVPLTNVYRNEFLKHTDFPIKLCAYSACFRREAGSYGKDVRG
                     LNRLHQFDKVEIVQLSLPENSFQILEEMTEHVAHLLRKLELPFRKVKLCGGDMSFTSA
                     LTYDFEVFSAAQQKWLEVSSVSNFTEFQANRMKLRFKDKNGKTRLAHTLNGSALALPR
                     IMAALLENNQSSQGIHIPE"
BASE COUNT          632 a          375 c          436 g          539 t
ORIGIN      
        1 ttcaaaaatt gagacgagta agaaatttta atcgagataa aatgtacgca attgtagaaa
       61 tagccgggca acaaataaag gttgaaacca agcgtgaata ttatgtcaat cggttgcaag
      121 gtgatgaagc aactcaggtt atcttcgaaa atatattgtt gattgatgat gattcgacca
      181 taagagttgg caggccaaga gtggaaggag cccgagtaaa ggcgacgatc tctgagcata
      241 taaaatcgga taaagtgctt atatttaaga agaaacgtag gaaaggatat caaaagctga
      301 atgggcatcg tcagccctta tctaaaatta taattgaaga aattagtgtt gcttaacttt
      361 taaatattca atttatatgg ctcataagaa aggtgccgga agttctcaaa atggcagaga
      421 atcggaaagc aaacgactgg gtgtaaagat tttcggaggt caatttgcca aagccggaca
      481 gattattgta agacaaagag gaacaattca tcatccgggg attaatgttg gcttgggtaa
      541 agaccatacc ttatttgctc tatccgatgg aattgtcgag tttcatcgac gtaaaaacaa
      601 taaatcgtac gtgtcggttt tccccgacaa taagaaagaa aactaaatct acttgagtta
      661 aaacatgata aggctcccgg cattctaacc ccgggagttt tttattatcg ctttagacct
      721 aattttgcaa tcttaaattt tattcagaat gattcccttg cagattattc gtgaacaacc
      781 cgactgggtt atacaaagat tggcggtcaa gaatttcgac gccaatagtc ttatctatca
      841 gatactcgaa ctcgattgga agagaaaatc cactcaattc gaatcggatg aaagatcggc
      901 tgagtataga aaactgactc aggaaatcgg ttcattgata aaagcagggc aagccggcaa
      961 ggccaatgat cgaaaaaatg aagttgcaaa aataaaggaa gaggtaagga acctgcagga
     1021 aagactcgat gaaatttcac gacagatgaa tgaacttttg gttcgcttgc ccaatattcc
     1081 cgatgagaga gtatcgcctg gtatttctcc ggccgataat gaaatcgtat attcggaagg
     1141 cagtattcct caacttcccc ctgaagctct ttgccattgg gatttagctg taaagtatca
     1201 gatcatcagt ttcgaaattg gttcgaaagt tactggtgcg ggttttccat tttacaaaga
     1261 caaaggagcg aaactccagc gtagcttgat cagttttttt ctggatgaag ccactaaggc
     1321 gggttatacc gaatacgaac cccctctcct ggtgaatgag gattcggcct ttgctactgg
     1381 ccaattaccc gataaggaag gtcagatgta ttttgttccc caggataatt tctatttgat
     1441 tccaacggct gaagtccctc tgactaatgt ttaccggaat gaattcttaa aacataccga
     1501 tttcccgatt aaattatgtg catattctgc ctgtttccgg cgcgaagctg gttcatacgg
     1561 aaaggacgta aggggattaa atcggcttca ccaattcgat aaagtggaaa tagtacaact
     1621 ttcattaccc gaaaattcct ttcagattct cgaagagatg acagagcatg tagcccattt
     1681 gctacgcaaa ctcgagctcc ctttccgaaa agtaaaacta tgtggaggag atatgagttt
     1741 tacctcggcc ttaacttatg atttcgaggt tttttcggcg gctcaacaaa aatggttaga
     1801 agtaagctcg gtgtcgaatt tcaccgaatt tcaagccaat cgaatgaagc tgcgttttaa
     1861 ggataagaat ggaaaaaccc gcctggctca caccctgaac ggaagtgcct tggcattgcc
     1921 tcgtatcatg gctgccttgc tcgaaaataa tcagtcatcc caaggtattc atatccctga
     1981 ag
//
LOCUS       DAORPD010000294         1982 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_10557_length_1982_cov_2.129735, whole genome shotgun sequence.
ACCESSION   DAORPD010000294 DAORPD010000000
VERSION     DAORPD010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10557_length_1982_cov_2.129735"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="PK976_04290"
     CDS             complement(<1..186)
                     /locus_tag="PK976_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRC67138.1"
                     /translation="MKRARPELNTGSMADISFLLLTFFLLTSSIDTDLGILRRLPPPP
                     DPTQEQVDIKKRNIFTVL"
     gene            complement(207..539)
                     /locus_tag="PK976_04295"
     CDS             complement(207..539)
                     /locus_tag="PK976_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67139.1"
                     /translation="MSSTLIDIGIYITYALALVALVSVIVFPLMSVFKDFKQAKGGLL
                     GFLGIIALIVIAILLSPADQGPFYDKFSIGPTLSKAIGGTIIATYILFGGLILSILYF
                     EVVKWFKK"
     gene            complement(546..1343)
                     /locus_tag="PK976_04300"
     CDS             complement(546..1343)
                     /locus_tag="PK976_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRC67140.1"
                     /translation="MKKLIALFSIAGMLTFGIYNTVSAQDTTKAQPQTEQTEEAAPAQ
                     SLTPPPAESQSFHQVLKEKFIEGGVPWMTPILIVLIFGLALTIERIIYLNLSTTDTRK
                     LLHKIENTLDAEGVEAAKEICKNTRGPVAGVFFQGLDRINEGLDVVEKSVVSYGGVLV
                     SKLENNLSWITLFIAIAPMLGFLGTVVGMVQAFDAIEAAGDISPTVVAGGMKVALLTT
                     VFGLIVALILQVFYNYLLSKVESIVNDMEDSTISFMDILVRYKNTKK"
     gene            complement(1411..1498)
                     /locus_tag="PK976_04305"
     tRNA            complement(1411..1498)
                     /locus_tag="PK976_04305"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1462..1464),aa:Ser,seq:gga)
     gene            complement(1658..>1982)
                     /locus_tag="PK976_04310"
     CDS             complement(1658..>1982)
                     /locus_tag="PK976_04310"
                     /inference="COORDINATES: protein motif:HMM:NF036996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-asparaginase 1"
                     /protein_id="HRC67141.1"
                     /translation="TNLQTFGTGNVPTDHRFLNVLGEAIKRGILIYNVSQCKAGSVAQ
                     GLYKTSLALESMGVISGADITTESAIAKMMFLFGQNYPREEICRLLSLPLRGEMSLTG
                     QKISN"
BASE COUNT          633 a          395 c          350 g          604 t
ORIGIN      
        1 caaaacagta aatatattcc tctttttaat atcaacctgt tcttgagtag gatccggtgg
       61 agggggcaaa cgtcttagaa tacctaaatc cgtgtcaatt gaagaagtta acaggaaaaa
      121 ggttaacaag aggaaggaga tgtctgccat tgaaccggta ttaagttctg gccttgcacg
      181 tttcatgttg attccatttt taaggtttat ttcttaaacc attttacaac ttcaaaataa
      241 agaatagaaa gaattaatcc tccgaaaagg atataagtag caataattgt acctccgatt
      301 gcctttgaaa gagtaggacc aatggaaaat ttatcataga aaggtccctg atcggctggg
      361 gatagtaaga ttgctattac aataagagca atgattccca aaaaacctaa tagccccccc
      421 ttagcctgct taaaatcctt gaatacagac attaaaggaa aaacaataac agacacaagg
      481 gcaactaagg ctaatgcata cgttatataa atgccaatat ctattaatgt agatgacatg
      541 ggtttttatt ttttagtgtt tttatatcta accaaaatat ccataaacga tatagttgaa
      601 tcttccatat cgttaacaat ggattctacc ttacttagga gataattata aaatacctgc
      661 aaaataagag caacaattaa accgaaaacg gtagttaaca aagctacttt catcccacca
      721 gcaacgacgg ttggagaaat gtcaccagct gcttctatag catcaaatgc ctgtaccata
      781 cctacaactg ttcccaagaa tccaagcata ggcgcaatag caataaacaa ggtaatccaa
      841 gaaagattgt tctcaagttt actcaccaaa actccaccat aagatactac tgatttctcg
      901 actacatcca aaccttcatt tatacggtct aatccttgaa agaagactcc agctacagga
      961 cctcttgtgt ttttgcaaat ttcttttgct gcctccacac cttcagcatc aagtgtattt
     1021 tcaattttat gtaaaagttt acgagtatct gtagttgaca agttcagata aataattctt
     1081 tcaatcgtaa gcgccaaacc aaagataaga acgattaaaa taggagtcat ccaaggtaca
     1141 ccaccttcga tgaacttttc ctttaaaact tgatggaaac tctgagattc ggcagggggt
     1201 ggtgttaagc tctgggctgg agcggcttcc tcagtttgtt cggtttgggg ttgggccttg
     1261 gtagtatctt gggccgaaac ggtattgtag ataccgaaag ttagcattcc tgctatcgaa
     1321 aataaggcaa tcaacttttt catagggcaa tgaattggat ttttaatgac taatttagtt
     1381 tgtagttttt caattttaag ttaatttttg cggagagaaa gggattcgaa cccttggtac
     1441 gctttcggcg tacacacact ttccaggcgt gcgccttaga ccactcggcc acctctccga
     1501 atccataacg atttgaattt taatgatccc atctaatgga aattcggctg ctaatttata
     1561 aagtttttta tttgtctgaa aaaacttcaa aaagttttta aaattattaa cggattctca
     1621 ttacaacgat aagatgagtc gtttatttta aatatcctca gttcgaaatc ttttgtccgg
     1681 taaggctcat ttcacctctc aaaggcaagc tgagcaatct gcatatttct tctcttggat
     1741 aattctggcc aaaaagaaac atcatttttg ctattgccga ttctgttgta atatccgctc
     1801 cactaatcac tcccatgctc tccaaagcca aactcgtctt gtataaaccc tgagctacac
     1861 ttcccgcttt acactgtgac acattatata taaggattcc tcttttgata gcttcgccaa
     1921 gaacatttaa aaaacgatgg tcggttggta cattgcccgt tccaaaggtt tgaaggttgg
     1981 tc
//
LOCUS       DAORPD010000295         1977 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_10592_length_1977_cov_2.275234, whole genome shotgun sequence.
ACCESSION   DAORPD010000295 DAORPD010000000
VERSION     DAORPD010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10592_length_1977_cov_2.275234"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..892)
                     /locus_tag="PK976_04315"
     CDS             complement(<1..892)
                     /locus_tag="PK976_04315"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67142.1"
                     /translation="MKQKFCALFFLIGTTLAATAQQGGYSLKFDGTNDYVNVPDNVSL
                     NFGTNPFTIEAWVKSTENTDNETDQLGDIITKFDQSNKKGFNLGFLRQSAHGSQSNYR
                     NLYFGIDNNHLDISWVEKASQAGNTEQHILSLVVYNGKLFGGTGENGMLYEWNGIDAW
                     VQKAPKLNDETEIISLAVYNGKLYGGTNPNGKLYEWNDTDAWVEKAAKLGDEIEIFSL
                     AVYNGKLYGGTYPNGSLYEWNGTDAWVEKAPKLSTETFIGSLAVYNGKLYGGTYPNGK
                     LYEWNGADAWVEKASKLGDET"
     gene            complement(1299..>1977)
                     /locus_tag="PK976_04320"
     CDS             complement(1299..>1977)
                     /locus_tag="PK976_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HRC67143.1"
                     /translation="EAARLFLWQKQLSYADLILTVSEDDSLYLRKKFPHKPVECIPSF
                     HGNDEVTSKAGFGNFALYHGKLSVPENDQSARFLIRKVFAGLPYRLIVAGMNPEKNLI
                     RLAKNYPNVVVMANPSLEEMDKLIEEAHINVLITQQNTGLKLKLLNTLFKGRHCLVND
                     KMLVGTNLAQLCHVANDKNSLKEKITELFQQPFSPDDIGKRIHHLNHWYSDDENTRKI
                     LDLVFKF"
BASE COUNT          577 a          397 c          340 g          663 t
ORIGIN      
        1 cagtctcatc accaagtttt gatgctttct caacccacgc atcagctccg ttccactcgt
       61 atagcttgcc gtttggataa gttcccccat aaagctttcc attataaact gctaatgacc
      121 caatgaaagt ctcagtgcta agttttgggg ctttctcgac ccaggcatca gtaccgttcc
      181 attcatacag acttccattt ggataagttc ctccataaag ttttccatta taaactgcta
      241 atgaaaaaat ctcaatctca tcaccaagtt ttgccgcttt ctcaacccag gcatctgtat
      301 cgttccattc atatagcttt ccgtttggat tagttccgcc ataaagtttt ccattataaa
      361 cagctaatga tataatctca gtctcatcat taagttttgg ggctttctga acccatgcat
      421 ctataccgtt ccattcatat agcatgccat tttctcctgt tccaccaaaa agttttccat
      481 tataaactac taatgacaaa atatgttgct ccgtattacc agcttgtgat gctttctcaa
      541 cccagctaat atctaaatga ttattatcaa tcccaaagta aagatttcga taatttgact
      601 gcgacccatg agctgattgc ctcaaaaaac caagattaaa tccttttttg ttagattggt
      661 caaatttggt aataatatca cccaactggt cggtttcatt atctgtattt tcagtggatt
      721 ttacccacgc ctcaattgta aaaggatttg ttccaaagtt taagctaacg ttgtccggca
      781 cattaacata atcatttgtg ccatcaaact tcagggaata accaccttgt tgtgccgttg
      841 ccgctaaggt agtacctatc aagaaaaata aggcgcaaaa tttctgtttc ataattttag
      901 tgttttattg gttaagaaat ttaataaata tctaactttt aaataattaa ctaaatatgg
      961 tgttgtaacc aataaaactc ttgtttctcc tcctaccttt tattcatcgt atcaatcttc
     1021 ctttcaatga gaaaaccagg gttttatttt aagtaaaggg aaagtaatct gcctacatta
     1081 gtaattgacg acaaaattaa aacataaata tgtatttgtt aagtgtttta cgaaaaaaaa
     1141 tgaaaaatat ttttcaacaa tttgtaaaat actggatatc agtgattggt gttgtccgtc
     1201 ttcagtcggc gtgaaaaaat gtaaaacccc acagttttat aaaatccgtc agggttaggt
     1261 gtgaaccggc tgttacgtaa attttgtgag gtttcgtctt agaacttaaa aaccaaatcc
     1321 agaatttttc ttgtattttc atcatccgaa taccagtgat tcaggtgatg aatccttttt
     1381 ccaatgtcgt cgggtgagaa gggttgttga aataattcgg tgattttttc tttcagagag
     1441 tttttatcat tggctacatg gcatagttga gccaaattgg ttccgacgag catcttgtcg
     1501 tttacaaggc agtgccttcc cttgaagagt gtattcagaa gctttagttt cagtcctgta
     1561 ttttgttgtg taatcaatac attgatgtgg gcttcttcga tgagtttgtc catttcctcc
     1621 aaggaaggat tggccatcac cacgacatta gggtaatttt tggcgagcct aatcaagttc
     1681 ttttcgggat tcattccggc aacaatcaag cgataaggaa gtccggcaaa tacctttcga
     1741 ataagaaacc gagccgactg atcattttct ggcaccgaaa gttttccgtg ataaagcgca
     1801 aaattaccaa aaccggcttt gcttgttacc tcatcgttgc catgaaaact gggaatgcat
     1861 tcgactggtt tatggggaaa tttttttctt agataaagag aatcatcttc cgaaactgta
     1921 agtataagat cggcatacga aagttgcttt tgccacagga ataatcttgc cgcttcc
//
LOCUS       DAORPD010000296         1975 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_10618_length_1975_cov_1.917708, whole genome shotgun sequence.
ACCESSION   DAORPD010000296 DAORPD010000000
VERSION     DAORPD010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10618_length_1975_cov_1.917708"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="PK976_04325"
     CDS             complement(<1..639)
                     /locus_tag="PK976_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRC67144.1"
                     /translation="MNQQSIKIFLVEDDLNFGAILKSYLELNDYEVIWVNDGKDAMTA
                     FYQGKFDICILDVMLPNIDGFSIAKEIKHFRPDIPLIFLTAKTLKNDVLEGFKLGADD
                     YITKPFDSDVLLYKIRAILKRHGQVSQKEEQREFQIGEYKFDAFLRIITHNDESRKIS
                     PKEAELLKMLCMNMNDIVPRELALKTIWGDDNYFTTRSMDVFITKLRKYLSKD"
     gene            complement(620..>1975)
                     /locus_tag="PK976_04330"
     CDS             complement(620..>1975)
                     /locus_tag="PK976_04330"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRC67145.1"
                     /translation="RFERMLPFANLSADEYYNAIIRRQERFFREQRQMMDKFDSVFMR
                     YQGRIISDRPKPRPQKLNPNPAFQKQVEDLQSQTTLLSDALDRLIFEIKQLEMPARER
                     LDVPASHEILKQELKKHNIDLPFEYAIFDEERDTLVRSPRFNPLSSTKVYEANLFTDR
                     FFQSDDHIALYFPDRSTHLLRSMLWIITGSALFTLIIVITFIISILVILRQKKISDVK
                     SDFINNMTHEFKTPIATISLAADSLVNARIINDPEKIHYFTRVIKEESKRMNTQVESM
                     LQMALLEKKDFSLHLQPMDAHDLMAQAVQNISIQIHKRGGEIHTDLQATNPIVEVDEV
                     HFLNVLFNLLDNANKYSPQSPQIEVNTWNGHHSLFISVKDHGMGMDRETRNRAFEKFY
                     RKSTGNIHNVKGFGLGLSYVKAIIMASGGNIHIQSEPGNGSEFIIELPQINNDDESTI
                     D"
BASE COUNT          559 a          368 c          408 g          640 t
ORIGIN      
        1 gtctttgctc agatacttgc gtaatttggt aataaacaca tccatgcttc gagttgtgaa
       61 ataattgtca tctccccaaa ttgttttcaa tgcaagttca cgaggaacga tatcgttcat
      121 attcatacat agcattttga ggagttcagc ttcctttgga ctgatcttgc ggctttcgtc
      181 attatgggta atgattcgca gaaatgcatc aaatttatat tcgccgatct ggaattctcg
      241 ctgttcttcc ttttgagaaa cttgaccgtg ccttttcaga atggctctga tcttataaag
      301 taaaacatcg ctatcgaagg gttttgtaat ataatcgtcg gcgcccagtt taaacccttc
      361 taaaacatca tttttaagtg tttttgctgt aagaaagatg agggggatgt cggggcgaaa
      421 atgtttgatt tcctttgcga tgctaaaacc gtcaatattg ggtagcatca cgtcgagaat
      481 acaaatgtcg aatttccctt gataaaaagc tgtcatggca tccttgccat cgttcaccca
      541 tattacttcg taatcattga gctcgaggta tgatttaagg atagctccaa agttaaggtc
      601 gtcttcaacc agaaatattt taatcgattg ttgattcatc gtcattgttt atttgaggca
      661 gttcgataat aaattcgcta ccgtttccag gttcactttg tatatgtatg tttccaccgg
      721 aggccattat gatggctttt acataactca aacccaatcc aaatcctttg acgttatgaa
      781 tgtttccagt agattttcga taaaattttt cgaaggcacg atttcgggtt tctctatcca
      841 tacccattcc atgatcttta accgaaatga ataaagaatg atggccattc catgtattta
      901 cctctatttg aggagattgt ggagaatatt tgttggcatt atccaaaagg ttgaataata
      961 cattcaggaa atgaacttca tcaacttcga cgatcggatt tgtagcctgc aaatcggtat
     1021 ggatctcacc tcctcgtttg tgaatctgga tggagatatt ttgaacagcc tgagccatca
     1081 agtcgtgtgc atccataggt tgcagatgaa gtgaaaagtc ttttttctcc aggagtgcca
     1141 tttgcagcat gctttcaacc tgtgtgttca ttcttttgct ttcttcctta attacgcggg
     1201 tgaaataatg aatcttttcg ggatcattga taatacgtgc gttaacgagg ctatctgcgg
     1261 ccagagaaat cgtagcaatt ggagttttaa attcatgcgt catattatta ataaaatctg
     1321 atttaacatc ggaaattttt ttctgacgca gaatcaccag aatacttatg ataaaggtaa
     1381 taactattat cagggtaaaa agcgccgatc ctgtgataat ccaaagcata gaacgcagca
     1441 aatgggtgct gcgatcggga aaatacaaag cgatatgatc gtccgattgg aaaaaacgat
     1501 cagtaaagag attagcctca tagactttag tgctggataa agggttgaag cgagggcttc
     1561 ggacgagggt atccctttcc tcatcaaaga tggcatattc aaaaggtaga tcgatattgt
     1621 gttttttcaa ttcttgtttc aaaatttcat gcgaagctgg cacatcaagc cgttcacgag
     1681 caggcatttc gagttgtttt atttcgaaaa tcagacgatc aagcgcatcg ctaagtagag
     1741 tggtttgcga ttgaagatct tcgacttgct tctgaaatgc gggatttgga ttaagcttct
     1801 gaggccgtgg tttgggacga tcggaaatta ttctcccttg ataacgcata aatactgaat
     1861 cgaatttatc catcatctgt ctttgttctc taaaaaatct ttcctgacgt ctgatgatcg
     1921 cattataata ttcatctgca gagagattgg cgaaaggtag catacgttcg aaacg
//
LOCUS       DAORPD010000297         1969 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_10674_length_1969_cov_2.331243, whole genome shotgun sequence.
ACCESSION   DAORPD010000297 DAORPD010000000
VERSION     DAORPD010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10674_length_1969_cov_2.331243"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(976..1452)
                     /locus_tag="PK976_04335"
     CDS             complement(976..1452)
                     /locus_tag="PK976_04335"
                     /inference="COORDINATES: protein motif:HMM:NF025491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4293 domain-containing protein"
                     /protein_id="HRC67146.1"
                     /translation="MIQRIQTIYLLIAVIATPILLFFFPLAGYFSDMAVIKLFLTHVQ
                     NLVPSGEMPLKLNPFLQYTGTFIAIIAGAFILYALFTFKNRIKQVKLVRISLFLIILM
                     IAWQFFITDLIKKNLGIIPNYELGIYMPIISLIFLLLAQRAILKDERKVHAADRLR"
BASE COUNT          730 a          314 c          341 g          584 t
ORIGIN      
        1 gacaaagacg aaattgttat caagactcac caagtggttc aacccgaaag tgccaccgga
       61 ttgtcgtaat tcttcaaaac ttcagaaagt tttcgcttaa tagtaaattc gcataatttt
      121 tcatccccct tcggtttgaa tggaatttca tcaacactca aaattcagaa aggggatttt
      181 taattgatca caaattttta aaaagccttc actcgatatt atatcccgaa atactccttt
      241 ttgccctaat tttagaatga aacccgcaag ctcttttctt atatcgaata aactatcagc
      301 tcgatctgaa tccaagcact ttccccatgc cagccgaaat ccccaacgct cagacaaaca
      361 tgtagaaagt tttacaaaaa ttgacgtaaa attcgaaaac aaaataaatt cagaatttcg
      421 aattgagatg atttgaatga atacatacag gtaaggatca atgatatgtt ttaaattcga
      481 taataaagaa gcataagata aattatccac atagatataa caaaagaagt aaaatctcat
      541 gtagataaga ccaatttcag taaaactgac ttaacgagtg aatgcaggga aaaaacaaga
      601 cttccgtaga aactagtttt cacgctgtgc catgtataaa gcaagtctcc cctcaaaata
      661 gatcaatatg gctgaaagtg tttcataacg atttcaaaaa tttcactgta gatggaacat
      721 tcagctcaaa ggcaagcata taacgaaact tcacagggtt atctaaaaat ttattaaaaa
      781 gggatcatta gatgtttcaa acgaagataa ttttcgtgat atgaattaaa atagacttga
      841 taaaggaagg agtttattcc acaatctacc tgtttctgtg aaaggaaaag caggttattc
      901 gaaacaagga gcgagaaata atgattcgaa aactcctgtc ttgccagacg atgaaattca
      961 tttcgaagta aataatcagc gaagcctgtc ggcagcatga actttccttt cgtctttcaa
     1021 aattgcgcgc tgcgccagta aaaggaaaat cagagaaatg atgggcatat aaatgcctaa
     1081 ttcataattg ggtatgatgc caagattttt cttaatcaaa tcggtaatga agaactgcca
     1141 agctatcatc agaatgatga gaaataaaga gattcttacc aattttacct gtttgatacg
     1201 gttcttgaaa gtaaataaag cataaagaat aaaagctccg gcaataattg cgataaatgt
     1261 tccagtatat tggagaaaag gattcaattt taacggcatt tcacctgaag gaacgagatt
     1321 ctgaacatgc gtgagaaata attttatgac ggccatgtca ctaaaatatc ctgcaagcgg
     1381 gaaaaagaaa agcagtatag gtgtggcaat tactgctatc aacagataaa tggtctgaat
     1441 tcgttgaatc attggttcag gttttttgca aaattaaaac gaagattcaa aagtactgaa
     1501 tttttgttga attacttaaa aattctcttg agcattcatc agaaaaaatc ctatttgatt
     1561 tgaatggggt atctgtttaa atttttttca aaaaaaggtt caaaatgctg aaaagtttat
     1621 aaaaacttct tacctttgca gcattaaaat ttgcctcctg cacatctcgg taaaatttct
     1681 aatgattttt tgatataata tggaggatag aaatatattc gattgaagca gtggaggtta
     1741 taaatagttt acttctaata tcagttttta aatagttttg aagaagagaa ggtggtaata
     1801 tccactgaag aagaaaatat ttggctatat actttcaaca ctcttaacat gtataatctt
     1861 caagagctaa atgaaaaatt tttgcccgaa ttgagggaaa tcgctcagtc gttgcatatt
     1921 gaggacatag acaaattatc gaaaatggat ttgatctaca agattatcg
//
LOCUS       DAORPD010000298         1965 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_10713_length_1965_cov_2.391099, whole genome shotgun sequence.
ACCESSION   DAORPD010000298 DAORPD010000000
VERSION     DAORPD010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10713_length_1965_cov_2.391099"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="PK976_04340"
     CDS             complement(<1..705)
                     /locus_tag="PK976_04340"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRC67147.1"
                     /translation="MKKYILLSIVLGHFASMFAQHILSGKVTTQAGEPLPGATIYCYE
                     LSKGTFSDSDGNFELSNLPAGKITVQFSFVGYASQVQIVLMDENVKRIDVALHETALE
                     AEEVVVSGGYNATQHQTAVKIDVLNLDEKRRKISPNIMETLSQVPGVNMISKGSGVAK
                     PVIRGLSMNDILTLNNGVRIENYQYSDHHPLGVNEFGIEDVEIIKGPASLLYGSDAIG
                     GVINFIREKPAPVGTLQ"
     gene            complement(1140..1475)
                     /locus_tag="PK976_04345"
     CDS             complement(1140..1475)
                     /locus_tag="PK976_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007134823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRC67148.1"
                     /translation="MKSADLQYPQCPIRNVLARFSDKWSLLILTTLHEKGMLRYSEIC
                     KSIPDISQKMLSTTLKRLEEDGLINRVAFAEIPPRVEYSLTEMGESLIPIFSQLIDWS
                     LLHFREIVR"
     gene            1702..>1965
                     /locus_tag="PK976_04350"
     CDS             1702..>1965
                     /locus_tag="PK976_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009131832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRC67149.1"
                     /translation="MKNLIVKNVDEIQGQTNAEHGNKFTVKTIIPEEEVSKCRVNFVE
                     VDPGNYAYGYHYHEMNEEVFYVISGQGAVRTEDGEIMLKAGDVI"
BASE COUNT          619 a          360 c          412 g          574 t
ORIGIN      
        1 ctgcaatgtc ccgaccgggg caggtttctc gcgaatgaaa ttgataacgc ctccgatcgc
       61 atcagatccg taaagaagcg aagcagggcc tttgattatc tccacatctt ctattccaaa
      121 ttcattcact ccaagcggat gatggtcgga atactgataa ttttcgatgc gtacgccatt
      181 gttgagcgtc aggatatcat tcatagaaag cccccgaata acgggcttgg caacaccact
      241 gcccttcgaa atcatgttga caccgggtac ttgcgataaa gtttccatta tattcggaga
      301 aatttttcgg cgcttttcgt caagattcag aacatctatt tttacggcag tttggtgctg
      361 tgtggcgttg taacctcctg aaaccacaac ctcttcggcc tcgagggcag tttcatgcag
      421 tgctacatcg attcttttga cattttcatc catcaaaaca atttgtactt gcgaagcata
      481 accaacaaat gaaaactgca cggtaatttt tccggcagga agattggaga gttcgaaatt
      541 gccatcggaa tccgagaatg tacctttgct cagttcataa caatatatgg tggctccggg
      601 taaaggctct cctgcttgcg tggtaacctt gcccgaaagg atatgttgtg caaacatgct
      661 tgcaaaatgg ccaagcacga tgcttaagag gatatatttt ttcatttttc tttccttttt
      721 aaatcgtgaa ataattaggg ttcctattct ttgataaaat ctatcgatat gagatttcag
      781 gaaaaaggag gggcacgatg tgaaaaagga agattagtga aagaaaaggt gcaaggagtg
      841 ttatactgat ttttgtatcg atcgaaatga gtaagaactt ccaaatcgat tttctgaatc
      901 tgatcggtga agatcgagaa atgaaaatcg caaataggac agttttcgtg aaatgttggg
      961 caggattttt cggaatgaca gcaacccgtt ctttgatgat gatgttccag tttcaccacc
     1021 gaaggaaaca ggaaaatgaa gatcaaagaa agcgaaacat atcttgtgta gctgcgattc
     1081 atttctattt gagaataagc tttgatagac tattcttaga cagaagtagt cagtctcttt
     1141 tatcgaacga tttctctaaa atgtaacagc gaccaatcta tcaactgact gaatataggt
     1201 attaaacttt cacccatttc agtaagtgaa tattcaacac gaggaggaat ttctgcgaat
     1261 gccactcggt tgataagtcc atcttcctcc aatcttttca gagtggtcga aagcatcttt
     1321 tgagaaatat cgggaatcga tttgcaaatc tccgaatagc gaagcattcc cttttcgtgc
     1381 agcgtggtca aaatcaatag cgaccatttg tcgctgaatc gagccaggac atttcgaatc
     1441 gggcactgtg ggtattgcaa atcggcagat ttcattattt tctttttttc attcacaaaa
     1501 gtaaaaataa ttcctgagga tgttacgggt attaaagatt attactttca aaaagagatt
     1561 tattataaat attcttaaaa aaatatttcc tattcagctg ataatcaata tttcgaacga
     1621 tagtagttcg taacacacat ctttgctttg tagaaagtgt tttgctaaat ttgaaaggtc
     1681 aaacaattta aatagaaaaa aatgaaaaat ctaatcgtaa aaaatgtaga tgaaattcag
     1741 ggacaaacaa atgcggaaca cggcaataaa tttaccgtaa agaccatcat ccccgaagaa
     1801 gaagtgagca aatgtagggt aaatttcgtg gaagtggatc cgggcaatta tgcctatgga
     1861 tatcattatc acgaaatgaa cgaggaagtt ttttatgtca ttagcggaca gggagccgtt
     1921 cgtaccgaag atggagaaat tatgctgaaa gccggtgacg ttata
//
LOCUS       DAORPD010000299         1965 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_10714_length_1965_cov_2.329843, whole genome shotgun sequence.
ACCESSION   DAORPD010000299 DAORPD010000000
VERSION     DAORPD010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10714_length_1965_cov_2.329843"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..612)
                     /locus_tag="PK976_04355"
     CDS             complement(46..612)
                     /locus_tag="PK976_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67150.1"
                     /translation="MKKSSMLASTAHTCQFFPPKPAQTALLCPDTEYIAGMMIIVIRD
                     IPAVYPNRSTENVTDETLKIHFASKVVSQIHHLILHKNNRKYLLSKIVDAVIIGGSSK
                     SNYLEKKSIKSEGNFWCTYGMTETLSHEAIRKIYPEEQGNYLASYFHHPFIITSLPDD
                     SKGEILLLMIEYDQAPDEKTMNEWNEMK"
     gene            complement(1366..>1965)
                     /gene="menA"
                     /locus_tag="PK976_04360"
     CDS             complement(1366..>1965)
                     /gene="menA"
                     /locus_tag="PK976_04360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase"
                     /protein_id="HRC67151.1"
                     /translation="FGVWLILEGLHYRISIMALIYLLMGFAAILAAIKYTVGRNPYGY
                     VGLGDLFVFIFFGPVGVAGTYYLHTNTWDPCVWLPSISLGLFSVAVLNINNMRDLSSD
                     ASWGKKTIPILLGFQKAKIYHLFILLIADAAFFVYIYYRQTSILQWLTLLNIPWFVLH
                     LTKVFKIKTQKELDPELKKVIMGTIFTVILYGIGSIWVI"
BASE COUNT          639 a          365 c          382 g          579 t
ORIGIN      
        1 agaaggaact atagaggtga gaatttcatt tcatttcatt tcatttcatt tcatttcatt
       61 ccattcattc attgtttttt catcgggagc ttgatcgtac tcgatcatca gaaggagaat
      121 ttcgcctttc gaatcatcgg gcagggaagt tatgatgaag ggatgatgaa aataagatgc
      181 aaggtaattt ccctgttcct cgggataaat ttttcgtatt gcctcatgcg aaagagtttc
      241 ggtcatgcca taggtgcacc aaaaattacc ttcagatttt atcgattttt tttcgagata
      301 attgctttta gacgaaccac cgataatgac tgcatctact attttactaa gaaggtattt
      361 tctgttattc ttatgtagga tcaaatgatg aatctgtgaa accaccttag atgcaaaatg
      421 aatttttagg gtttcatcgg taacattctc tgtcgatcta tttggataaa cagccggtat
      481 gtctcttatt actattatca tcattcctgc aatgtattca gtatcgggac aaagcagggc
      541 agtttgtgcg ggcttcggcg gaaaaaactg gcaggtatgg gctgtcgaag ccagcataga
      601 agattttttc acccgaataa ttttaagctt acacttagaa ccggaattct gaataaaaat
      661 ttcatcggaa tcgctgttcc agttattgat aaaatttccg ggaagtctat gccagtttta
      721 atgtgttggg tcgctgagct tgctgaccaa tccaataagt taattacctg cataaatttt
      781 tccgttcagg gtgagagaaa aggagataca agggttaatt cactttcgat caaatcatgc
      841 aagttgcatt ttcagcgggt agataatata gagatgacct ctgtctcatc gcgaaaaaag
      901 aaccaaaatt attgatatac aaaattcccc ttcctgaaat ttatctcagt tggttactca
      961 gggcaaaggc aaactgcgcg atgacattca acccaaataa gatcaatgat gagggtggat
     1021 ttattgtcta ggacaaaatt cgaccgaaac aaaatccgat tcccaccagt taagaggtca
     1081 agcaatggag tttcataagc ccttcgtagg gaaggaaaaa ccattgaaga aaccatacac
     1141 ttcagccaat tgaggtgatc tacataaaga tcgaagtttt tcaataaaag caggaggaat
     1201 atcgggtctg ggttaaagca gcgggctacc ttcgcctatg ctcataagtc tggggtttag
     1261 catcatcaca aaggcatagg aaataactgt ctttgggaat aagcttgctt ctttatgttt
     1321 ctacaggttt ttttaaatat aaatttccta taaacgtttt tgactttaaa ttacccatat
     1381 cgagccaatt ccgtaaagaa tgacagtaaa aatagttccc atgattactt tcttcaattc
     1441 cggatcgagt tctttctgag tcttaatttt aaacactttg gtcaaatgca agacaaacca
     1501 gggtatattc agcaaagtca gccattgcaa aatcgaagtt tgtcggtaat agatatagac
     1561 aaaaaaagcc gcatcagcaa tcaaaagaat gaatagatga tagatttttg ctttttgaaa
     1621 gccaagcaag atgggaatgg tttttttgcc ccaggatgca tcgcttgata gatccctcat
     1681 attattgata ttcaatacag caacactaaa caatcccaat gagatggaag gcagccaaac
     1741 acaaggatcc catgtattgg tgtgtaagta atacgtacca gcaaccccaa cgggtccaaa
     1801 aaatatgaaa acgaaaagat cacccaatcc aacataacca taagggttgc gaccaaccgt
     1861 atatttgatg gctgctaaaa tggctgcaaa gcccattaat aaataaatta aagccataat
     1921 gcttatgcga taatgcagac cttctaaaat gagccagact ccaaa
//
LOCUS       DAORPD010000300         1964 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_10722_length_1964_cov_2.589838, whole genome shotgun sequence.
ACCESSION   DAORPD010000300 DAORPD010000000
VERSION     DAORPD010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10722_length_1964_cov_2.589838"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            180..>1964
                     /locus_tag="PK976_04365"
     CDS             180..>1964
                     /locus_tag="PK976_04365"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRC67152.1"
                     /translation="MIRKLLFTLGIILATHVLVFSQATGTLRGRIIDKETKEPIPFAN
                     VIIEMNGVQAGGATSDFEGNYVIKPINPGKYDIRCSYVGYKTLLTKGIIIGAGKIEFY
                     NIELEPTTVALSEIEVTSYKVPLIEKDQTTVGATVTAEQIDKMPNRSATAVAITVGGV
                     FSSDGERGSVRGARSEATVTYIDGVKVTGSASLPPSAIEQVSVIISGLPAQYGDATSG
                     VINITTKGPSNIYAGGFEAQTSELLDNFGYNRFGFNLQGPLIRKKNKETGESRSILGF
                     FFAGDVILQGDAAPSPIGRWKVKDDVLQYLKENPLRPTGLGYGTYPNAAFLASDDLEY
                     VKRSQNADAFNINLSGKIDLRLSSTTNFTLGGSTVYYKSNAFGYSRSLMNWENNPFHD
                     ELTYRGYARFTQRFPAAADSRSLIKNVYYSLQADYTKYHSRTQSAQHKDNLFKYGYLG
                     RFDTYTVRSYELGSDTINGQVYNNVYIHNGYRDTMVTFQPSDFNRDLARYTETYYNFY
                     PDVTGNWENFDQILLGGGYINGMSPSAIYSLWQAPGTVYNGYSEYDADQIGFNGMLSA
                     DIGNHEVQIGFQMEQRSESYYGYAPTYFW"
BASE COUNT          611 a          408 c          408 g          537 t
ORIGIN      
        1 tgacaaaatt tttactgagt atagaaaagt ctataattaa ttcataatca ataaataaca
       61 acctcaaaat attctcgaaa atttcaaata taaaacaaag gtttgtaaaa taaacttaaa
      121 tctttctaat tttaccgact ttaaatgcac aacgcaaatc atcaataaat ctatctggta
      181 tgataagaaa actactattt acactgggta ttatactggc aacccacgtg ctggtatttt
      241 cccaggcaac gggtaccctg agggggagaa taattgacaa ggagacgaaa gaacccattc
      301 catttgccaa tgtcatcatc gaaatgaatg gcgtacaggc tggtggtgcg acttctgatt
      361 tcgaaggaaa ttatgtcatc aagcccatta atcccgggaa atatgatata aggtgttctt
      421 acgttggtta caaaaccctt ctaactaaag gcattatcat aggtgcgggg aaaatcgaat
      481 tctataacat cgaattggag cccaccactg tagctttaag tgaaattgag gttaccagct
      541 ataaagtacc tctgatagag aaggaccaaa ccactgtggg agctacggta actgcggaac
      601 agatcgataa gatgcctaat cgttccgcta cagctgtggc aataaccgta ggcggtgtgt
      661 tttcatctga cggtgagcga ggcagtgtta gaggtgcacg aagcgaagcc actgtaactt
      721 acatcgacgg agtaaaagtt acaggatcgg ccagcctgcc tccttcagct atcgaacagg
      781 tttcggtaat tatttcggga ttaccggcac agtatggcga cgccacctcc ggcgttatca
      841 atatcaccac caaaggtcca tccaacattt atgctggagg tttcgaggct cagacttccg
      901 aactcctcga caactttgga tataaccgat ttgggttcaa cttgcaaggc cctctcatcc
      961 gtaagaaaaa taaggaaact ggcgaatcga gaagcatctt gggatttttc tttgctggcg
     1021 atgtgatttt acagggagat gctgccccca gccctattgg aagatggaaa gtcaaagacg
     1081 atgtattaca ataccttaag gaaaatcctc ttcgacccac gggtttaggc tatggaactt
     1141 accccaatgc cgcattcctc gcttccgatg acctcgaata tgtgaaaaga tctcaaaatg
     1201 ccgacgcttt taatattaac ctttcgggaa aaatagattt gcgcttatcc tcaactacca
     1261 attttacgtt gggtggttcg acagtgtact ataaaagcaa tgcctttgga tactcccgtt
     1321 cgttgatgaa ctgggaaaat aatcctttcc atgatgaact gacttatcgt ggttatgcca
     1381 ggtttacgca gagattccca gccgcagccg atagcagatc tctcataaaa aacgtttatt
     1441 atagccttca ggcagactac acaaaatacc attcaagaac tcaaagcgcc cagcataaag
     1501 ataatctatt caaatatgga tatttgggac gattcgatac ctataccgta cgttcttacg
     1561 aattgggaag cgatactatt aatgggcaag tttataataa tgtatatata cataatggct
     1621 acagagatac catggtaacc ttccaaccct ccgattttaa cagagatctg gcacgctata
     1681 ctgaaaccta ctataatttt tacccggatg ttaccggcaa ttgggaaaat ttcgaccaaa
     1741 ttcttctggg aggtggttat atcaatggaa tgagtcccag tgccatttat tctttgtggc
     1801 aagcccccgg aaccgtttac aatggatata gtgaatatga tgccgatcag attggattca
     1861 atggtatgtt atcggccgat attggtaatc atgaagttca gataggattc caaatggaac
     1921 aaagaagtga aagctattat gggtatgccc ctacctattt ctgg
//
LOCUS       DAORPD010000301         1963 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_10732_length_1963_cov_2.251572, whole genome shotgun sequence.
ACCESSION   DAORPD010000301 DAORPD010000000
VERSION     DAORPD010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10732_length_1963_cov_2.251572"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            75..>1963
                     /locus_tag="PK976_04370"
     CDS             75..>1963
                     /locus_tag="PK976_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRC67153.1"
                     /translation="MEYNFREIELKWQKYWSNNKTFKTENASSKPKFYVLDMFPYPSG
                     AGLHIGHPLGYIASDIYARYMRLKGYNVLHPMGYDAFGLPAEQYAIQTGTHPEITTRK
                     NIERYRKQLDRLGFSFDWDREIRTCDPSYYKWTQWTFIQLFNSWYDKKLDKACPISEL
                     MEYFSLNGNEGIEAVSGKVPSFSAQYWNSATEKTRSEILMEYRLAYQSETIVNWCSEL
                     GTVLANDEVSDGLSVRGGYPVERVKMKQWLLRITAYAERLLEGLEKIDWSDSIKDIQR
                     NWIGRSEGCTIFFKLADYEDAIEIFTTRPDTIFGATFMVLSPEHPFAEKITTTSHLYD
                     VSSYITWAKNRSERERMAEVKRVTGVFTGAYAINPFNNKKIPVWIADYVLAGYGTGAI
                     MAVPAHDSRDFIFARHFQLPIIQVVKKPNEEILDINLCDSSYDAKEGIMINSGFMNGM
                     DVNDAIKAAVAKVEEMGIGHRTISYRLRDAIFSRQRYWGEPFPVYYKDGIPYTLPENE
                     LPLELPLIDAFLPTTNGEPPLARAKNWNYKGYPLETNTMPGFAGSSGYYFRYMDPHND
                     KAYFSHEAVNYWRNVDLYIGGSEHATGHLIYSRFWCKFLYDIGLAVEDEPFQKLINQG
                     MIQGR"
BASE COUNT          585 a          392 c          422 g          564 t
ORIGIN      
        1 cattaaaacc caagacagga tagagcgaaa cgttgtaatg tcctactttt gcaatttcaa
       61 attttgtata gtgaatggag tataatttca gagaaatcga gctcaagtgg caaaaatact
      121 ggagtaataa caagactttc aaaaccgaaa atgccagtag caaacctaaa ttttacgttc
      181 tggacatgtt cccctatcct tcgggtgctg ggttgcacat aggacatcct ttgggatata
      241 tcgcttcaga tatttatgcc cgatacatgc gactgaaagg ctacaatgtc ttgcatccta
      301 tgggttatga tgcctttggg ttgcccgccg aacagtatgc cattcaaaca ggaactcatc
      361 ctgaaattac tacccgtaaa aatatcgaac gctatagaaa gcaattggat agattaggtt
      421 tttctttcga ttgggacaga gaaataagaa cctgcgatcc ttcttattat aaatggacac
      481 aatggacttt tatacagctt ttcaattcct ggtacgacaa gaaactcgac aaagcttgtc
      541 ctatcagcga attaatggaa tatttttcat tgaatggaaa tgagggcata gaagcagtct
      601 ccggcaaagt tccctctttt tcggctcaat actggaattc ggccactgaa aaaacacgtt
      661 cggaaatctt gatggaatat cgtctggctt atcaatcgga aaccattgtc aactggtgtt
      721 ccgagctggg cactgtttta gctaacgatg aagtaagtga tggattatct gtgaggggtg
      781 gctatcctgt cgaaagagtt aaaatgaaac aatggctgct tagaataacg gcttacgccg
      841 aacgcttgct cgaaggcctc gaaaaaatcg actggagcga ttccatcaag gatatccaaa
      901 gaaactggat aggtaggagt gaaggatgca ccatattctt caagttagct gattatgagg
      961 atgctatcga aatttttacg actcgccctg atacgatatt cggcgcaaca tttatggttc
     1021 tgagccccga gcatcctttc gcagaaaaaa ttactacgac ttcacacctt tatgacgtat
     1081 cttcctatat tacatgggct aaaaaccgat ctgagcgtga acgtatggca gaggtgaaaa
     1141 gagtaacagg ggtgtttaca ggtgcttatg ccatcaatcc attcaacaat aaaaagatac
     1201 ccgtttggat tgctgattat gtattagccg gatatggaac gggggctatt atggctgttc
     1261 ctgctcatga tagccgagac ttcatctttg ctcgccattt tcaacttcct atcatccaag
     1321 tagtaaaaaa acccaacgaa gaaatcttag atatcaatct ttgcgattcc tcttatgatg
     1381 caaaggaagg aatcatgatc aacagtggtt ttatgaatgg tatggacgta aatgatgcta
     1441 ttaaggcagc tgtggctaaa gtagaagaaa tgggtattgg tcataggacc atcagttatc
     1501 gcctgcgcga tgctattttc agtcgtcagc gttattgggg cgaacctttc cctgtttatt
     1561 ataaagatgg catcccttat acattgcctg aaaatgaatt gcccttagag ttacccctta
     1621 tcgatgcctt cttgccgact acaaatggtg agcctccttt ggctagagct aaaaattgga
     1681 attataaggg ttatccactc gaaaccaaca ctatgccagg cttcgccggc tccagtggtt
     1741 attatttccg ctacatggat cctcataatg ataaggcgta tttctcacat gaagcagtta
     1801 attattggag aaatgtcgat ttgtatatag gcggttctga acacgctact gggcatttga
     1861 tctattcacg cttctggtgt aaatttcttt acgatatagg tttagccgta gaagacgaac
     1921 ccttccaaaa actcatcaat caaggcatga ttcaaggtcg tag
//
LOCUS       DAORPD010000302         1961 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_10751_length_1961_cov_2.610178, whole genome shotgun sequence.
ACCESSION   DAORPD010000302 DAORPD010000000
VERSION     DAORPD010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10751_length_1961_cov_2.610178"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1961)
                     /gene="dnaE"
                     /locus_tag="PK976_04375"
     CDS             complement(<1..>1961)
                     /gene="dnaE"
                     /locus_tag="PK976_04375"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HRC67154.1"
                     /translation="LGILSKRCDVPLIATNDVHFINKSDFEAHKILIKLNTDPKNLET
                     DTNLVYTGNEYLRSPDEMAEIFKDFPEAIENTVSLAEKVEKYKLKRSILLPRFPLPEE
                     FTDENEYLRYLTFEGSQRKYPEMTDEIKQRLDYELEVIKNMGFSGYFLIVQDFINAAR
                     RMDVIVGPGRGSATGSAVAYAIGITNIDPIRYNLLFERFLNPERISMPDIDVDFDDEG
                     RQNVLNYVAEKYGEDHVAQIITFGSMAAKSAIRDVARVIGLPLSEADRLAKLIPDRLG
                     IKLQEAIEEVPELEQAAKGSDEKIQKVIEYARILEGSVRQTGTHACGVIIGPDDLKKY
                     IPMATQKDSQLLVTQFEGKSMESVGMLKMDFLGLKTLSIIREALHNIKLNRSITIDID
                     NIPLDDPLTFQLYQKGDTIGTFQFESEGMRMYLRELKPEHIEDLIAMNALYRPGPMQY
                     IPMYINRKKGREKVTYPHDMLRNILEDTQGIMIYQEQIMQVAQKMGGFTLGSADILRR
                     AMGKKDPVVMAQQKEIFIQGALNKGVTVETASEVFETMLKFAQYGFNRSHSTAYSILA
                     YQTAYLKANFPAEYMAAVLTHNLNDIKKITYYIDECQRMGINVLGPDVNESQLNFFVN
                     KKNEIRFGLTAIKNVGENAANSIIKERENG"
BASE COUNT          494 a          424 c          392 g          651 t
ORIGIN      
        1 ccattttctc tttcttttat gatggaatta gctgcatttt cgccaacatt tttgatggca
       61 gtaagcccaa accttatttc atttttttta tttacaaaaa agtttagttg actctcattc
      121 acatcaggcc ccagtacatt tattcccatt ctctgacatt catcgatata ataggttatt
      181 tttttaatgt cgttcaagtt atgggttagg actgcagcca tgtattctgc aggaaaattt
      241 gccttgagat aagctgtttg atatgccaga atagaatagg cagtagagtg tgatcggttg
      301 aagccatatt gggcaaactt gagcatagtt tcaaagactt cggaagctgt ttcgacagta
      361 actcctttat ttaacgcacc ttgaatgaag atttcctttt gttgtgccat tacaaccggg
      421 tcctttttcc ccatcgctct gcgaagaata tcggcgcttc cgagagtaaa acctcccatt
      481 ttttgtgcta cctgcataat ctgttcctga taaatcataa ttccctgagt gtcttccaga
      541 atgttgcgaa gcatgtcatg aggataggta accttttcgc gtcctttttt tcgattgata
      601 tacatgggga tatattgcat ggggccggga cgatataaag cgttcatggc aatcaagtct
      661 tcgatatgct cgggtttcag ttccctcagg tacatgcgca ttccttccga ttcgaattga
      721 aatgtgccta tggtatcgcc tttttggtaa agttgaaatg tcaagggatc atcgagcgga
      781 atgttgtcga tgtcaatagt aatagagcga ttgagcttga tgttgtgtaa tgcttctcga
      841 ataatgctca aggtcttcag tcctaagaaa tccattttca acattcccac tgattccatt
      901 gatttacctt cgaactgggt aaccagtaat tggctgtcct tttgggtagc catgggtatg
      961 tatttcttca aatcatcggg gcctatgatt acaccgcagg cgtgggttcc agtttgcctt
     1021 accgagcctt ctaagattct ggcgtattca attacctttt gaatcttttc atcagatcct
     1081 ttcgccgcct gttcgagttc aggaacttcc tctatggctt cctgcaattt aatgcccaac
     1141 ctgtcgggta tcagttttgc cagtcggtca gcttcagaaa gaggcagccc tattaccctt
     1201 gcaacgtctc tgatagccga ttttgctgcc atacttccaa aagtaataat ttgtgctaca
     1261 tggtcttctc cgtatttctc tgctacgtaa tttaaaacgt tttgccggcc ttcatcatcg
     1321 aaatctacat cgatatcggg catagatatt cgttcggggt tgagaaagcg ttcgaacaga
     1381 aggttgtaac gaatgggatc gatgttggta attccaatgg cataagctac agccgagcct
     1441 gtagctgagc ctctgcctgg tcctacaatc acatccatcc gccgtgcagc attgataaaa
     1501 tcctgcacga taagaaaata tcccgaaaat cccatgtttt taatcacttc cagctcgtaa
     1561 tcgagcctct gcttgatttc gtcagtcatt tcagggtatt ttctttggct accttcaaaa
     1621 gtcaaataac gtaaatattc attttcatca gtaaattctt caggaagtgg aaaacgagga
     1681 agcaagatac ttcgtttcaa cttgtatttt tccacttttt cggctaagct aaccgtattt
     1741 tcgatggctt cgggaaaatc cttgaaaatc tctgccatct catccgggct ccttaaatat
     1801 tcattccccg tatataccag atttgtgtcg gtttcgagat tcttcggatc agtatttagc
     1861 ttgatgagaa ttttatgcgc ttcgaaatcg ctcttattga tgaaatgaac atcgttggta
     1921 gcaatcaacg gaacatcgca tcttttcgaa agtatgccaa g
//
LOCUS       DAORPD010000303         1960 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_10759_length_1960_cov_2.051444, whole genome shotgun sequence.
ACCESSION   DAORPD010000303 DAORPD010000000
VERSION     DAORPD010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10759_length_1960_cov_2.051444"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..>1960)
                     /locus_tag="PK976_04380"
     CDS             complement(61..>1960)
                     /locus_tag="PK976_04380"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HRC67155.1"
                     /translation="GANISVKLTDEFMEAVKNNREYVHQFPIDAEKPSFTKTVYARQL
                     WDKIIHNAWKSAEPGILFWDTIIRESIPDCYADYGFRTISTNPCGEIPLCPYDSCRLM
                     AINLYSYVENPFTPQAFFNAELFTVHVRKAQRIMDDIIDLELEKIDRILEKITQDPED
                     EETKRIERLLWENIRHKAVLGRRTGLGITAEGDMLAALGYRYGSDEAINFCVAVQRTL
                     ALEAYRSSVEMAVERGPFEIYDSQREENNPFILRLKEADEELYHNMVKHGRRNVSLLT
                     VAPTGSVSILTQTSSGIEPVFAVRYKRRRKINPSGFPVSKISYTDISGDSWEEYNVFH
                     HGFIRYLQVKGYNPDEVLQLSDKEIDKLIEDSPYYNSTANDIDWVAKVRMQGNIQKWV
                     DHSISVTVNVPAETSEELVSKIYLTAWESGCKGVTIYREGSREGVLIKEKKEEEMVIH
                     ENNAPRRPKVLEASVVRFLNGSEEWVAVVGIYNGRPYEIFTGKAEGFYAPQWVTHGWV
                     IKNRQPDGKARYDFQFEDKDGYRTTMEGLSRMFDAEYWNYAKLISGILRHGMPLTSVI
                     DLVSKLRLDSDSINSWKTGVERALKQFIPNGTKVAKATCEKCGQTGTLQYQEGCLICT
                     SCGYSKCG"
BASE COUNT          499 a          457 c          393 g          611 t
ORIGIN      
        1 cctcaacctt gacattcact tttgttgatt ccttcgatgt atcataataa aaaacacctt
       61 ttaaccacat ttcgaataac cacatgaggt acaaatcaaa catccttcct gatactgaag
      121 cgttccggtt tgtccacatt tctcacaggt tgccttggcc accttggttc cattgggtat
      181 gaactgttta agggctcttt cgactccggt tttccatgaa ttgatcgaat cggaatcgag
      241 tcttagtttc gataccaaat ctattactga agtaagaggc attccatggc gcaggatacc
      301 cgaaatcagt ttggcataat tccaatattc tgcatcgaac attcttgaaa gcccttccat
      361 ggtggtgcga tatccgtcct tatcttcgaa ctgaaagtcg taacgagcct tgccatcggg
      421 ttggcgattt ttgatgaccc atccatgggt aacccattgt ggagcataaa agccctctgc
      481 ttttcccgta aagatttcat aaggtcgccc attataaatt cccaccactg ccacccattc
      541 ttcagagccg ttcaagaatc ttactacgct ggcttccagt actttaggac gacgcggggc
      601 attgttttca tgtatcacca tttcctcttc tttcttttct ttgatcagaa caccttctct
      661 ggatccttca cgataaatgg taacaccttt gcaacccgat tcccatgctg ttaaatatat
      721 tttactcact agttcttcgc ttgtctcggc aggaacattt accgttacgc taatggaatg
      781 atctacccat ttttgaatgt ttccctgcat tctaactttg gctacccagt caatatcatt
      841 ggctgttgaa ttataatagg gtgaatcttc gatgagtttg tcgatttctt tgtcggagag
      901 ttgcaaaacc tcgtcgggat tgtaaccctt aacctgtaaa tatcggatga aaccatgatg
      961 aaatacattg tattcttccc aggaatcgcc cgatatatcg gtataggaga ttttactgac
     1021 agggaatcca gaggggttta tcttgcggcg tcgtttgtat ctgacggcaa aaacaggttc
     1081 gatccccgat gaagtctgcg taagtatgct gacgctcccc gtaggggcta ctgtgagcaa
     1141 tgagacattt cggcgaccat gtttcaccat gttgtggtaa agttcctcat cggcttcttt
     1201 caaacgaagg ataaaaggat tgttttcttc tcgctgcgag tcgtatattt caaatggccc
     1261 tctttctacg gccatttcta ctgaagagcg ataagcttcg agagctaatg ttctttgcac
     1321 agccacgcaa aaattaatgg cttcatcact tccataacga taacccagtg cggccagcat
     1381 atcaccttcc gcggttatac ctaaacccgt ccttcgtccc aatacggctt tatgacgaat
     1441 gttttcccat agcaaacgtt ctatgcgttt ggtttcttca tcttcgggat cttgagtaat
     1501 cttttcgagt atcctgtcga ttttctctaa ttccaaatcg atgatatcgt ccatgatgcg
     1561 ttgtgctttc cttacatgca cagtaaaaag ctcagcattg aagaaggcct ggggggtaaa
     1621 gggattttcg acataagaat aaaggttgat ggccatcaga cgacagctat cgtaagggca
     1681 gagtggaatt tctccacagg gattggtgct tatcgtgcga aaaccataat cggcataaca
     1741 atcaggtatg gattccctta taatggtatc ccagaacaat atgccgggtt cggctgattt
     1801 ccaagcatta tgaatgatct tatcccatag ctgacgtgca taaacagtct ttgtaaatga
     1861 tggtttttcg gcatcgatag gaaattggtg aacgtattcc cgattgtttt tcaccgcctc
     1921 cataaattca tcggtcaatt ttaccgagat attagccccc
//
LOCUS       DAORPD010000304         1959 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_10773_length_1959_cov_2.161765, whole genome shotgun sequence.
ACCESSION   DAORPD010000304 DAORPD010000000
VERSION     DAORPD010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10773_length_1959_cov_2.161765"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..935)
                     /locus_tag="PK976_04385"
     CDS             complement(<1..935)
                     /locus_tag="PK976_04385"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRC67156.1"
                     /translation="MMNSFKILKQVILLMLLFFLPFLGYGQEIHEWTLEECITYARNH
                     NINVKKQMLNVQLQKINTTESKLTMLPTLGGNATHVYNWGQTIDPFTNQFATERVRSN
                     SFYVSTQMVLFSGFQKLNTLRKSQLDLNISELEVEKMMNDISLNVATFYLQALYNAEL
                     LNISESQLKVVKLQRDRIKILVESGSLAQGELLNMEAQVASAELQLVEARNNYELALL
                     QLSQLIDLEDSRGFSVVAPELAAPSVNISLSPVDQVVNYALQHQPEIISSKYRIESAR
                     KEFDINRGSYGPTLALNGSWGTGYSGVSLEKDPNVP"
     gene            1231..>1959
                     /locus_tag="PK976_04390"
     CDS             1231..>1959
                     /locus_tag="PK976_04390"
                     /inference="COORDINATES: protein motif:HMM:NF037667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67157.1"
                     /translation="MMRKIRISMILLLTGILSSVQAQQLKSDTFDVVHYDLHLDIMNF
                     QAKQLNGFAILTLTPKMNQLSYISLDLLSLQVDSVKVEGQPVVSWYQDDTLLRIPLIS
                     PVSVGDTFQMRIRYHGTPIVEPAGWGGFHFDSWIAYNLGIAFQANPHNYGRAWFPCID
                     DFIDRATYDYYITTESGKTAVCGGLLIDSIVHPDNSITWHWKMNQTIPAYLASVAVAS
                     YIKIADIYNGIQTDIPISLYFRPSD"
BASE COUNT          548 a          460 c          365 g          586 t
ORIGIN      
        1 ggcacattgg ggtccttttc gagcgaaact cccgaataac ccgttcccca actgccgttg
       61 agtgcaagcg tcggaccata acttccgcga ttgatgtcga attcttttct ggcagattct
      121 attctgtatt tcgaagaaat gatttcgggt tgatgctgca aggcataatt aaccacctga
      181 tcaacgggcg aaaggctgat atttaccgaa ggagccgcca actcaggagc tacaacacta
      241 aaaccgcgcg aatcttccag atcgatgagc tgagataatt gcaacaatgc cagttcataa
      301 ttattgcgag cttcaaccaa ctgcaactcg gcagatgcca cctgcgcctc catgttcaac
      361 aattcaccct gtgccagtga acccgattct actaaaatct ttatccgatc acgctgtaat
      421 ttcaccacct ttaactgact ctccgatata ttcaacaact ctgcattata caaggcctga
      481 agataaaatg tggcaacatt cagcgatatg tcgttcatca ttttttccac ctccagttca
      541 cttatattca aatcgagctg gctcttacgc aaggtgttga gcttctgaaa gccactaaat
      601 aacaccattt gcgtagaaac ataaaaactg ttagacctta ccctttcggt tgcaaactga
      661 ttcgtaaatg gatcgatggt ttgtccccaa ttgtacacat gcgtggcatt acctcccaaa
      721 gtgggcaaca tcgtcagctt cgattccgtc gtattaatct tctgaagctg aacattcaac
      781 atttgtttct tgacgttaat attgtgattg cgggcatagg taatacattc ttccagcgtc
      841 cattcatgaa tttcctgacc gtaacctaaa aaaggtaaaa agaaaagcag catcaataaa
      901 atgacctgct tcaaaatctt aaaggaattc atcatattta gaccaatttt caaattgtaa
      961 aatctaattt cctgagtttt gatacaattt atacttccac gtttagacga taaccgaaaa
     1021 atgatgttac attttcaggc tcaaaacgct tcaaaggtat gactttctgg tatagcacaa
     1081 aagaatgtga gatgaaatta tttattctcc ttggcaaaca tcgataaaca cccgatgaga
     1141 acttcatttc tttttttgaa ttttcttcaa gataagtcaa tgaattaata agagtggctt
     1201 atatttgtgc attgattcct ttagaactct atgatgagaa aaattcgcat ctcgatgatt
     1261 ttgttgctca cgggtattct atcctcggtg caggcacagc aattgaaaag cgataccttc
     1321 gacgtggtac attatgatct ccatttggac atcatgaatt ttcaggccaa acagctgaat
     1381 ggatttgcca ttctcaccct tactcccaag atgaatcaac tttcatacat ttcactcgat
     1441 ttgctgagtc ttcaggtgga ctcggtaaaa gtggaaggac agcctgtagt gtcatggtat
     1501 caggatgaca ctctgttgcg catccctttg atatcgccgg tttcagtggg cgataccttt
     1561 cagatgcgaa ttcgttatca tggcactccc attgttgaac ctgccggatg gggcggtttt
     1621 cacttcgatt catggattgc ctataacctt ggtattgcct ttcaggctaa cccacacaac
     1681 tatggtcgcg cttggttccc ctgtatagac gattttatcg accgcgccac ttacgattat
     1741 tatattacta ccgaatccgg caaaactgcc gtatgtggtg gcttgctcat cgattctatc
     1801 gtccatcccg acaacagcat tacctggcat tggaaaatga accagaccat cccagcttat
     1861 ctggcttccg tagccgtggc ttcctatatc aaaattgccg acatttacaa cggtatccaa
     1921 accgatattc cgatttcatt gtacttcaga ccttccgat
//
LOCUS       DAORPD010000305         1956 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_10788_length_1956_cov_2.579169, whole genome shotgun sequence.
ACCESSION   DAORPD010000305 DAORPD010000000
VERSION     DAORPD010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10788_length_1956_cov_2.579169"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="PK976_04395"
     CDS             complement(<1..390)
                     /locus_tag="PK976_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67158.1"
                     /translation="MEKKYKIWILVLSSVIIFLIVAFITLYARTQRIITGSAPIERPV
                     GQGRGMVIRYLQLDENQTNQYDQIRRQYNVQIASLRDSMLIIRKKMIQELKSDTPDTI
                     KLNYLALEIGRLEGEINMALNQHLLKLK"
     gene            complement(425..874)
                     /locus_tag="PK976_04400"
     CDS             complement(425..874)
                     /locus_tag="PK976_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67159.1"
                     /translation="MKIDCQNFNKWYPRYKSKRLSQQEIISSKQHLLKCTNCSTLAEE
                     IESAVESKVNFEPDPYFFTRFQARLNTQDINRSSTSWIFQFKPRNIFLYGFSIIFAVL
                     MGYYTGRILLPTSPTDELTLQEVLVMEDGIANEPLGLEMIFEESDLE"
     gene            complement(871..1419)
                     /locus_tag="PK976_04405"
     CDS             complement(871..1419)
                     /locus_tag="PK976_04405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRC67160.1"
                     /translation="MQENNQNAFRILVENFEPMIRSIGYQLFMQEEEVEDFTQEVFMK
                     IHLGISGFRKKSSLSTWIYRIALNHGLNSKRKNSAIPFSHTSHKIYAQALESENNILH
                     PRTFNHFVEKKETRKILNHALNKLPKNQRIAFILTHIEDLSYSEVAEIMEKTPKAIDS
                     LTQRAKNNLRKYLAKYYKLLKP"
     gene            complement(1605..>1956)
                     /locus_tag="PK976_04410"
     CDS             complement(1605..>1956)
                     /locus_tag="PK976_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67161.1"
                     /translation="TMMSLFPIAIMAQTTSKNQSQYKNIGSTPLTSPRFLDQNKDGIC
                     DNWPSRAWIQGRGAFIDKNGDGVCDNYGNRTGIRMNRYFVDANNDGVCDNYPSTFSGK
                     RGGHKGYKATQLSR"
BASE COUNT          543 a          346 c          381 g          686 t
ORIGIN      
        1 tttaagcttc aaaagatgtt gattcagggc catatttatt tctccttcga gtctgccgat
       61 ttctaatgcc agataattta attttatcgt atcgggagtg tctgatttca attcctgaat
      121 catctttttt cgaataataa gcatcgaatc gcgtaaggat gcaatctgaa cattgtactg
      181 gcgtcgaatt tgatcgtatt ggttagtttg gttttcgtcg agttgtaaat atcggataac
      241 cattccccta ccttgaccca ctggcctttc aattggagcg gaacccgtga ttatacgttg
      301 ggttctggca tatagagtga tgaatgccac aattaaaaag attattacag atgaaagcac
      361 caaaatccaa atcttgtatt tcttttccat ttatcgaatt attagccttt aaaaaaactt
      421 taaatcattc taagtcactc tcttcaaaaa tcatctcgag ccctaaaggc tcattcgcta
      481 ttccgtcttc cataaccaaa acctcttgaa gtgttaattc gtcggttggt gaagttggca
      541 agagaattct tccagtataa taacccatga ggacagcaaa gatgatactg aaaccataga
      601 gaaagatatt tctgggcttg aattggaaaa tccaggatgt agaagatcga tttatatcct
      661 gggtatttag gcgggcttgg aaacgagtga agaaataagg atcgggttcg aaatttactt
      721 ttgattcaac agccgattcg atctcttcgg ccaatgtcga acagttggtg catttcaaca
      781 gatgttgttt tgacgatata atttcttgct ggcttaacct cttcgattta taccgtggat
      841 accatttatt gaagttctgg caatcgattt tcatggcttt aggagtttat aatacttggc
      901 aagatatttt cgtaagttgt tttttgctcg ttgtgtaagc gaatcgatgg cttttggagt
      961 tttttccatt atttcggcta cttcagagta ggacaaatcc tcgatatgcg tcaaaataaa
     1021 tgcaattctc tggttttttg gaagtttatt caaggcatgg tttaaaattt ttcttgtttc
     1081 ctttttttct acaaaatgat tgaaagttct gggatgaaga atgttatttt ctgattccag
     1141 ggcttgtgca taaattttat gcgaagtatg tgaaaaagga atagcactat ttttacgttt
     1201 cgagtttaat ccatgattca gggctatgcg atatatccat gtggatagag aagacttttt
     1261 acgaaaccct gagataccga gatgaatctt cataaaaacc tcttgggtaa aatcttccac
     1321 ttcttcttct tgcataaaga gttggtatcc aattgaccta atcatgggtt caaaattttc
     1381 gaccaggata cgaaatgcat tttggttgtt ttcttgcaaa gcctctatca aggaggattc
     1441 atctattttc tgtttacaaa taatagtcta tttttttcga gatgatttat ttgcaaactt
     1501 aatgataaca tcttgagata aacattttta ataggggagc aaatcgtggc ttgggaaggg
     1561 attcctcagc agaatcgtat cagcgattgg gaatggtttg attattacct cgagagttga
     1621 gttgctttgt agcccttatg tcctccacgt ttacctgaga atgtgctggg atagttgtcg
     1681 cacactccat cgttgtttgc gtcaacaaaa tatctgttca ttcttattcc tgttctgttt
     1741 ccatagttgt cgcatactcc atctccattt ttatcaataa aggctcccct tccctgtatc
     1801 caagctcttg atggccaatt gtcgcaaatt ccatccttgt tttggtctaa gaatcgagga
     1861 gaagttaaag gagtagaacc aatattttta tattgagact gattctttga agttgtttga
     1921 gccataattg caatcggaaa caagctcatc atcgtt
//
LOCUS       DAORPD010000306         1955 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_10797_length_1955_cov_2.198421, whole genome shotgun sequence.
ACCESSION   DAORPD010000306 DAORPD010000000
VERSION     DAORPD010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10797_length_1955_cov_2.198421"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="PK976_04415"
     CDS             complement(<1..440)
                     /locus_tag="PK976_04415"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HRC67162.1"
                     /translation="MSQLSSHSQKRYVVSGIIIAVAAIFILKLFYMQVISDKYKILSE
                     SNVRRIVTIYPARGLIYDRNGRKIVSNEEAYDVMVVPRQVRNIDTAELCQLLEIDMQS
                     FTNRLNKARQYSPYRASPFVEQISKTTYGYLQEKLYRFPGFFVQP"
     gene            816..>1955
                     /locus_tag="PK976_04420"
     CDS             816..>1955
                     /locus_tag="PK976_04420"
                     /inference="COORDINATES: protein motif:HMM:NF028500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRC67163.1"
                     /translation="MKLIETPIEYLKGVGPVRGDLLRKELQIFTFEDLLYYFPFRYED
                     RTIIYRIKSLNPSMNYVQVIGKVEMMETLGSGRNLRLHVYINDGTGRLELVWFQGLKW
                     AKQLFKSDLTYLVYGKLNYFNGTYSIPHPEVEIYDPSNGDDIKALYPVYSSTEKLKSR
                     GLGSKGISKLVKTLLLTLEGQIQESLDEMVIDEMNLISLSKALQFIHFPPDQFSLESA
                     RLRLKFEELFYAQLSVLQLKHQRKSFSHGHTFQKVGKFFNEFYEHHLPFALTNAQKRV
                     IREIRADLRGPRQMNRLLQGDVGSGKTLVALMVMLIALDNNYQTCLMAPTEILAQQHY
                     NTFLEMLSGMEVEVRLLTGSTRQAERQEILNGLLENRVQILLGTHA"
BASE COUNT          574 a          383 c          392 g          606 t
ORIGIN      
        1 ggttgtacga aaaaccccgg gaaacggtag agcttttcct gtaaatagcc ataggtagtt
       61 ttcgaaatct gttccacaaa gggcgaagct ctatacgggc tatattgtct ggctttgttt
      121 aatcgattgg taaaactttg catatctatc tcgagtaatt ggcatagctc ggccgtgtcg
      181 atatttctga cttgtcttgg aaccaccatc acatcataag cctcttcatt cgaaacgatc
      241 tttctgccat tcctatcata aatcaaaccc cttgccggat aaatggttac tatgcggcgt
      301 acattgctct ccgacagaat tttgtacttg tcgctgatta cctgcatgta aaataatttc
      361 aaaataaaaa tagcggccac ggcgatgatg atccccgaaa ctacatacct tttctgcgaa
      421 tgcgatgaaa gctgagacat attgcaaatt tattgggtat tccctgaact tttataatgg
      481 cgattcgtta tgaacttttt tgagccgaaa tctcaactac tgaatttttt gcccatcttg
      541 ttgcttttca tcgtatccaa catttcccag aataaagccc ttctttacaa gaagaatgtt
      601 acgggaaaat caatgaatta tttgttggat gattttatct ctttttacgg atgtattttt
      661 atatatcaat gttaatcagc aaagaagaat gactttcctt tatcggtatc cttttgaaaa
      721 aacaataagc gattttaaat aaacacctta tgtctacaat cttttattaa tcagcgataa
      781 gatttaattt tgcttattag cttatagccc aaaagatgaa attgatcgaa actcccatag
      841 aatatttgaa aggagtgggt ccggttcgtg gagacctctt acgaaaagaa cttcaaattt
      901 ttacctttga agatttgctc tattattttc cttttcgtta tgaagatagg accatcattt
      961 acaggataaa atccctgaat ccatcgatga attacgtgca ggttatcggt aaggtagaaa
     1021 tgatggaaac gctgggatcg ggtcgaaacc tacgcttgca tgtttacatc aacgatggaa
     1081 cgggtagatt ggagctggta tggtttcagg ggctcaaatg ggcaaagcag ctctttaaat
     1141 cagacctaac ttatctcgtc tatggtaaat tgaattattt taatggaaca tacagcatcc
     1201 ctcatcccga agtggaaatt tatgaccctt cgaatgggga cgatataaag gcactgtatc
     1261 cggtttattc ttctaccgaa aagttaaaaa gccgtggatt gggaagcaaa ggaatctcaa
     1321 aactggttaa aacccttttg cttaccctcg aaggacaaat acaggaaagt ctggatgaaa
     1381 tggtaatcga tgaaatgaat cttatctctt tatcgaaagc tttgcagttc atccattttc
     1441 cacccgatca gttttcattg gaatctgctc ggctacgctt gaaattcgag gagttgttct
     1501 atgcacagtt atccgttctt caattgaagc atcaacggaa gtcgtttagc catggacata
     1561 cttttcaaaa agtaggcaaa tttttcaatg aattctatga acatcatctt ccgtttgcat
     1621 tgaccaatgc tcaaaagcgt gtaattcgtg aaattagggc agatttaaga ggccctcggc
     1681 aaatgaatcg cttgctgcaa ggagatgtgg gcagtggtaa aaccttagtt gccttaatgg
     1741 taatgctcat tgcgctggat aataattatc aaacctgctt gatggctccc accgaaattc
     1801 tcgcacagca acattacaat acctttctcg agatgctttc gggaatggag gtagaagtcc
     1861 gtttgctcac gggcagcact cgtcaggccg aacgtcagga aattttgaat ggtctcttgg
     1921 aaaacagggt gcagattctt ttaggcacgc atgcc
//
LOCUS       DAORPD010000307         1949 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_10847_length_1949_cov_2.507392, whole genome shotgun sequence.
ACCESSION   DAORPD010000307 DAORPD010000000
VERSION     DAORPD010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10847_length_1949_cov_2.507392"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="PK976_04425"
     CDS             complement(<1..462)
                     /locus_tag="PK976_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRC67164.1"
                     /translation="MTEIRNIAIIAHVDHGKTTLVDRILYQGKLFRENQQIGELILDS
                     NELERERGITILAKNVSVRYKGVKINVIDTPGHSDFGGEVERVLNMADGVLLVVDAFE
                     GPMPQTRFVLEKALEMNLKPVVVINKVDKPGCEPELVQEAVFDLMFSLGASN"
     gene            complement(572..1372)
                     /locus_tag="PK976_04430"
     CDS             complement(572..1372)
                     /locus_tag="PK976_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67165.1"
                     /translation="MNEKKNKVSGIVATIVFHALFLLLMFTMALRTPLPLPEEEGVEV
                     NLGYSDEGFGSIQAAELPAGQRAAVIPLGSIKDEEIVTTNDEEIPSLSHKEKNIKKNK
                     QTTPLEQTGEKKSQEPVLNPNALYTGKLNGEGTVTNQGIAGGIGDQGRPDGTTGSNSY
                     TGTGGTGQGPGVYLSGRTKVFLYQPAYNSMEQGTVVVEITVDRQGNVIRAIPGKKVPN
                     TSIGTNTTDQELWKAAREAALKSKFSSNPNADPEQKGYIVYHFVKLEG"
     gene            complement(1369..1812)
                     /locus_tag="PK976_04435"
     CDS             complement(1369..1812)
                     /locus_tag="PK976_04435"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRC67166.1"
                     /translation="MAIQKRNKLNIAFNPAPMSDLVFLLLIFFVISSTLVSPNAIKLL
                     LPSSKSKTMAKQAITVYINDHFEYFVDPDGRGHAVPVNLPQLQQILAENLQKQDIDAS
                     VVLRADQSVPVQYVVSLFDIVNDINTQLNSKYKVILATRPVKEIQ"
     gene            complement(1824..>1949)
                     /locus_tag="PK976_04440"
     CDS             complement(1824..>1949)
                     /locus_tag="PK976_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRC67167.1"
                     /translation="LIVGILAYVCYNVLVARIEKLVFNLEARATDFMDLLQEPAA"
BASE COUNT          532 a          413 c          410 g          594 t
ORIGIN      
        1 gttcgatgct cccaatgaaa acatcaaatc gaaaacggcc tcctgcacta actcgggttc
       61 acatcctggc ttatctactt tgttgattac aacaacaggt ttcaaattca tctcaagcgc
      121 cttttcgagt acaaagcgcg tttggggcat gggaccttcg aaggcgtcca ccacaagcag
      181 aacgccatcg gccatattca acactctttc cacttctccc ccaaaatcgg aatggccagg
      241 agtatcaata acattgattt ttactccttt atatctcacc gaaacatttt tggccaatat
      301 ggtgatgccc ctttcccgtt ccaattcatt ggaatcgagg attaattctc caatctgttg
      361 attttcacga aacaactttc cctgataaag aatgcgatcg actaaagtgg ttttgccatg
      421 atcgacatgg gcaatgatgg caatattgcg tatttctgtc atgcaattta tcgaatcgaa
      481 aaaagtttgc aaaattacgg taaattgcaa caagatatat tccgaaatat ttcaaaaaag
      541 ggaaaaggat ggaggaattt ggatgaagta attatccttc gagttttacg aagtgataaa
      601 cgatatatcc tttttgctcg ggatcggcat tagggttgga agaaaatttc gatttaaggg
      661 cggcttcccg tgcagctttc catagttcct ggtcggttgt attggttccg atagaagtat
      721 taggaacttt tttcccagga atggcacgga ttacatttcc ctgacggtct actgtaattt
      781 cgaccacgac tgttccctgc tccattgagt tgtatgcagg ctgatagagg aataccttgg
      841 ttcgtccact caaataaact ccaggtcctt ggcctgtgcc tccagttcct gtataggaat
      901 ttgatccggt tgttccgtcg ggtcttccct gatcgcctat tcctcctgca ataccttgat
      961 tggttacggt tccctcccca ttcaatttcc cagtatataa ggcatttggg ttgagcacgg
     1021 gttcttgtga ctttttttca ccggtttgtt ctaaaggagt agtttgcttg ttctttttta
     1081 tatttttttc tttatggctt aaagaaggaa tttcctcatc attggtagtt acaatttctt
     1141 catcctttat cgagcctaaa ggtatcaccg cagctctttg gcctgcagga agttctgcag
     1201 cttggatact tccaaaaccc tcgtcgctat agcccaaatt cacctctacg ccttcttcct
     1261 ctggtaaagg aagcggggta cgcaaagcca tagtaaacat cagcaaaagg aatagagcgt
     1321 ggaatactat ggtggccaca atgccagata ctttgttttt tttctcgttc attgaatttc
     1381 cttaaccgga cgagtagcca aaatcacctt gtatttgcta ttgagttggg tattgatgtc
     1441 attcactata tcgaagagtg aaactacata ttggacaggt acagattgat cggctctcaa
     1501 aaccaccgaa gcgtcaatat cctgtttttg gaggttttcg gccaaaattt gctgaagttg
     1561 tggaagatta actggtacag catgaccacg accatcggga tcgacaaaat actcgaaatg
     1621 atcattgata taaaccgtaa tggcttgctt ggccattgtc ttactcttac tggaaggcag
     1681 gagaagttta atggcattgg gacttaccaa ggtagaagaa ataacgaaga aaatcaataa
     1741 gaggaatacc aggtctgaca tgggagccgg gttaaaggca atattcagct tatttctttt
     1801 ttggatggcc atattttagg aatttatgcg gcgggttcct gaaggagatc cataaaatcg
     1861 gtggctcttg cttcaagatt aaacacgagt ttttcgattc gagcaaccag aacattatag
     1921 cacacataag cgagaattcc aacgataag
//
LOCUS       DAORPD010000308         1949 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_10849_length_1949_cov_1.721753, whole genome shotgun sequence.
ACCESSION   DAORPD010000308 DAORPD010000000
VERSION     DAORPD010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10849_length_1949_cov_1.721753"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="PK976_04445"
     CDS             <1..711
                     /locus_tag="PK976_04445"
                     /inference="COORDINATES: protein motif:HMM:NF014714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HRC67168.1"
                     /translation="ITILPFESDFYSQHHFEVFYAGHPLLDVISEYIPPSREEFLSRN
                     SLNHQPIIAILPGSRKQEIKVMLPVMLSMVKRFPEFQFVIGAAPSLPDNFFEPITNHH
                     RIAITKGQTYDLLSHSYAALVTSGTASLETALLNVPQVICYKGQPLSYFIARRMVKIG
                     FIGLPNLIAGRQVVKELIQNYFTPARLYDEIDKIVKDERYRNEIRKGYDEIRHLLGGP
                     GASKRAANEIVRVIASQS"
     gene            708..1634
                     /locus_tag="PK976_04450"
     CDS             708..1634
                     /locus_tag="PK976_04450"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRC67169.1"
                     /translation="MKQYFKIILLLFISWNLIIESSAQNDAEYFIKQGITAARLGYTE
                     QALQNFNTAIYKDPTLPEAWYNRGVLYYKLGNFQKALFDMDQAARINSKQTNVFYQRG
                     LIYHALGNDSLAREDFRKTISLDTTMTDALFNLGLIDFEHQNYEQALMNFDKLVKSDS
                     KNTQALYHRGLCKHHLGRFFEAIADFDAALALLPNDAEIILSRGVAKAEAGYYYTAIE
                     DFTRVIYLNPNYAEAYFNRAMASFNLYKFAEAESDYNMAYSIDSSAEALTGRGLSRLA
                     QHQMDTGCADLKMAIELGYEPAIKYLMNYCKD"
     gene            1664..>1949
                     /locus_tag="PK976_04455"
     CDS             1664..>1949
                     /locus_tag="PK976_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67170.1"
                     /translation="MKHFLSIFLIALVVISACKSNSSKKELPKENLTATIHDTTQKSY
                     QPGQDAFKPGTSDTDENSQWTFTKAATLIGLGNYDEALNYMNQYIEKNPED"
BASE COUNT          601 a          418 c          364 g          566 t
ORIGIN      
        1 attactatac ttcctttcga aagtgatttt tattctcaac accattttga ggtattttat
       61 gcagggcatc ccttgctcga tgttatttcc gaatatattc ctccctctcg agaagaattc
      121 ctcagtcgaa attctttgaa tcaccagcct ataatagcaa ttttgccggg cagtcgaaaa
      181 caggaaataa aagtgatgtt acccgtgatg ctcagcatgg taaagcgttt ccctgaattc
      241 caatttgtga ttggagcagc tccttctctt cccgacaatt ttttcgaacc aattaccaat
      301 catcatagaa ttgcaatcac taagggacaa acctatgatt tgctgagtca ttcttatgca
      361 gctttagtta cctcgggaac tgcttcactc gaaaccgcct tactgaatgt gcctcaggta
      421 atttgttata aaggtcagcc cctttcttat tttattgcca gacgtatggt aaaaatcgga
      481 tttatcggct tacctaatct tatcgccggc cgacaagtag taaaagaact catacagaac
      541 tattttactc ctgcacgttt atacgacgaa atagacaaga tagtgaaaga tgaacgatat
      601 agaaacgaga ttcgcaaagg atacgacgaa attaggcatt tgttgggagg cccgggggct
      661 tcgaaaagag ctgctaatga aatcgtacga gtaattgctt cacaatcatg aaacaatatt
      721 tcaaaattat cttgttgctt tttatttctt ggaatctcat tattgaatct tcagcccaga
      781 atgatgcaga atatttcata aaacagggaa taacagcagc ccgtctggga tatacagaac
      841 aggccctcca gaatttcaac accgccatct ataaagatcc tacactgccc gaggcatggt
      901 ataatagagg tgtgctctat tacaagttag gaaacttcca gaaagccctt ttcgatatgg
      961 atcaggcagc cagaatcaac tcaaagcaaa ccaatgtatt ctatcaacgt ggtttgattt
     1021 atcacgctct gggaaatgat tccctggcca gggaagactt tcgaaagact atttcacttg
     1081 acactaccat gaccgacgcc ctttttaatt taggcctcat cgatttcgaa catcaaaatt
     1141 atgagcaagc actcatgaac ttcgataaat tagtcaaatc tgattcaaaa aatactcaag
     1201 ctttgtatca taggggttta tgcaaacatc atctgggaag gttcttcgag gccattgccg
     1261 acttcgatgc cgccttggcg cttttgccaa acgatgcaga aattatcctg agccgtggcg
     1321 tcgcgaaagc cgaagctggt tattactaca ctgcaatcga agattttacc cgagttattt
     1381 atttaaatcc caattatgcc gaagcatatt ttaaccgcgc tatggctagt tttaatcttt
     1441 acaaatttgc agaagccgaa tcggattaca acatggccta ttccatagat tcatccgctg
     1501 aagctctcac aggaagaggt cttagccgcc ttgcccagca tcagatggat actggctgtg
     1561 ccgacttgaa aatggccata gaacttggat atgaacctgc aataaaatac ttgatgaatt
     1621 actgtaaaga ttgataattt tgccttcttt aaactttcca gatatgaaac actttttatc
     1681 tatcttcttg attgccttag ttgtaatttc ggcttgtaaa agcaattcaa gcaaaaagga
     1741 actgcccaag gaaaatctaa ccgctaccat tcacgatact acgcagaaat cttatcaacc
     1801 cggacaagat gcatttaaac caggtacgtc tgacaccgat gaaaattccc aatggacatt
     1861 taccaaggca gctaccttga ttggcttggg taattatgat gaagccttga attatatgaa
     1921 tcaatatatt gaaaaaaatc cagaagatg
//
LOCUS       DAORPD010000309         1943 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_10898_length_1943_cov_2.703390, whole genome shotgun sequence.
ACCESSION   DAORPD010000309 DAORPD010000000
VERSION     DAORPD010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10898_length_1943_cov_2.703390"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(197..>1943)
                     /locus_tag="PK976_04460"
     CDS             complement(197..>1943)
                     /locus_tag="PK976_04460"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRC67171.1"
                     /translation="DYIRSMQNLGLLYFQNRMYDSAIQEFAKAISISDEMIKNQEPQS
                     PEYYRLLEQNADLHRLLALGWQNKGRFKLALQEMQRGITLAIMCNNTQLQASLYSDMS
                     QILQKQGIYEKALEYLIKAVKIYEKLGEKLMIANAYQTIGKIYIENSDYINAYRFFKN
                     SLQVHKSLDNKAGLATIYNDFGNLKRIFNETDSAEFYYKKSLEINKLLSNKQEMGINY
                     LNLGILSEERGKHSHARIYLEEAKKIFEQIDDEENLARVLLEQGLLEANQNHTSLAID
                     LLLKAEKMASNYGMIPIRKSAAKYLSDIYERTGDYFKAFQYQKLYSILSDTLNNIERQ
                     KQILSIQATYDFEKQQNELLLEQEKRKTLEKNQTLQRVISISSLLALILTGLITYLIF
                     KRIKEKQKIEAEKLLKEAEISKAKETLIETQLRNQDLEKQRLIEELKYKSNHLTNLAL
                     IIAQKNEFINELKNLLKELKNSNESQKDKVMKELWQKTTQQSNINKELDKFKKEIDDA
                     NRSFYEKLNNICPQLTLHEKELAGLLRINLSSKEIASLHNVSVKAIEMSRYRLRKKLQ
                     LESNDKLVDFLQKLD"
BASE COUNT          491 a          329 c          379 g          744 t
ORIGIN      
        1 caaaaataca agtttcctac cccacatacg tatttttata ttctatcata cttctacaag
       61 tcttggattt tggcatatta ttgagtgaat ttacctctac aattaattta actaaattat
      121 taaaaatcaa ttaatattca tataaaaaaa ttctacaaaa gtgaatttgc gaacattaga
      181 ataggcaaca gaattatcaa tcgagctttt gaagaaaatc taccaattta tcattgctct
      241 cgagctggag ttttttgcgc aaacgatatc ggctcatttc tatggctttt accgaaacat
      301 tatgcagtga tgcaatttcc ttcgatgaaa ggttgatacg taataagccg gcgagctcct
      361 tttcgtgcaa agtaagctga gggcagatat tatttagttt ttcgtagaaa cttcggttgg
      421 catcatctat ttctttcttg aacttatcca actctttgtt gatgttgctc tgttgggtag
      481 ttttttgcca gagttccttc atcactttgt ccttttggct ttcattgctg tttttcagtt
      541 cttttaagag gtttttcaat tcgttgatga attcattttt ttgagcaata atcagtgcta
      601 agttggtgag atgattgctc ttgtatttta attcctcgat aagtcgttgt ttttcgagat
      661 cttgatttct taactgtgtt tctatcagag tttcttttgc cttactgatt tccgcttctt
      721 tcaatagttt ttcagcttct atcttttgct tttctttgat tcgtttgaag atcagatagg
      781 taataaggcc cgttaaaatg agagctaata atgatgaaat agaaattact ctttgcaagg
      841 tttgattttt ttcgagagtc tttcgttttt cttgttcaag aagcagttcg ttttgctgtt
      901 tttcgaaatc ataagttgcc tggatgctga ggatttgttt ctgacgttcg atattattca
      961 gtgtatcgct caagattgaa tacagttttt ggtattgaaa ggctttgaag taatctcctg
     1021 ttctttcata gatatcggaa agatatttag cggctgattt tcgaatggga atcatgccat
     1081 agttcgaggc catcttctct gctttaagaa gtaagtcgat ggcaagggat gtatggtttt
     1141 gatttgcttc caagagtcct tgttcaagta gaactcgggc aagattttcc tcgtcgtcga
     1201 tttgttcgaa aatttttttt gcttcttcca gataaatgcg ggcatgtgag tgcttgcccc
     1261 tttcttccga taaaatgcca aggtttagat agtttatgcc catttcttgc ttgttggaga
     1321 gtaatttatt aatttcgaga cttttcttgt aatagaattc agccgagtcg gtttcgttga
     1381 atatccgttt taaattgcca aaatcgttgt atatggtagc gaggcctgct ttattgtcga
     1441 gagatttatg tacttgcaaa gagtttttga agaagcggta ggcattgatg tagtcgctgt
     1501 tttcgatata tattttgcct atggtttgat aagcattggc tatcatgagt ttttcgccga
     1561 gtttttcgta gattttaacg gctttaatca aatactcgag cgctttttcg taaatcccct
     1621 gtttttgaag aatctggctc atgtcggaat ataaactagc ttgaagctgc gtattattgc
     1681 acataatggc caatgtaatt cctctttgca tttcctgcag agccagtttg aatctgccct
     1741 tgttctgcca tcccaaggcc agcaagcggt gaaggtcggc attttgttct aaaagtcgat
     1801 aatattcggg gctttgtggt tcctgattct ttatcatttc gtcggaaatg ctgatggctt
     1861 ttgcaaattc ttgaattgcc gaatcataca tacggttctg aaaatacaac aatcctaaat
     1921 tctgcatgga tcgaatataa tcg
//
LOCUS       DAORPD010000310         1942 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_10907_length_1942_cov_2.888182, whole genome shotgun sequence.
ACCESSION   DAORPD010000310 DAORPD010000000
VERSION     DAORPD010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10907_length_1942_cov_2.888182"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="PK976_04465"
     CDS             <1..278
                     /locus_tag="PK976_04465"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012527.2"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HRC67172.1"
                     /translation="LEKGKRNVEILKQGLHQPLPVEEQIAIIYCGTHNLLKNVPVNRI
                     HDFETEYLNLLSEKYPDILQTIQTGQFPDEVANKLEEIVAKLVKRYE"
     gene            292..1170
                     /gene="atpG"
                     /locus_tag="PK976_04470"
     CDS             292..1170
                     /gene="atpG"
                     /locus_tag="PK976_04470"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HRC67173.1"
                     /translation="MSNLKEVRLRIESVKNTQQITNAMKMVAASKLRKAQNAILSLRP
                     YAQKMSELISTLYSNYSENLSSPFFEQHETDKVLLVSLASNRGLCGAFNSNIIKQIKY
                     LTETSFEQQYGLNHLKLISIGKHVSDILINKKYPVLQIKDNLIEKVTWEGVKLLADEL
                     IELWTKHEFDRIVLVYNQFINAATQVVTTETFLPLSYDENKKTRNIDYIFEPSSETII
                     EELIPHYLHIQLYKTFLDSFASEQGARMTAMMIATDNADVILKDLTLSYNKARQAAIT
                     KEILEIVSGSNALNKV"
     gene            1207..1707
                     /locus_tag="PK976_04475"
     CDS             1207..1707
                     /locus_tag="PK976_04475"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HRC67174.1"
                     /translation="MKKNIEQAIIEQIQKEEHSSRLYLQMAIWCEINGFPGAAAFLYK
                     HSDEERLHQLKFIHFLNDREGMALLKPLETPQSDYPSLKEMFEKILEHEKFISASINQ
                     IYGLTLQEKDYTTASFIHWFINEQIEEETLVRTILDKMKLAGDGQNRGLYLIDKELGV
                     MAASGH"
BASE COUNT          659 a          431 c          352 g          500 t
ORIGIN      
        1 tactggaaaa aggaaaacga aatgtcgaaa tcctcaaaca aggtcttcat caacccctgc
       61 ccgttgaaga acaaattgct atcatctact gcggtacgca caacctcttg aaaaacgttc
      121 cagtaaatcg catacatgat ttcgaaacag aatatctgaa tctgcttagc gaaaaatatc
      181 ccgacattct ccaaactatt caaaccggcc agtttccgga tgaagttgcc aataaattgg
      241 aagaaatagt tgccaaactg gtcaaacgat acgaataaaa aatattagcc catgtcgaat
      301 ctcaaggaag tcaggttaag aatcgaatcg gttaaaaata ctcaacagat caccaatgcc
      361 atgaaaatgg tagcggcttc gaaactgcgc aaagcacaga atgccatcct ttccctccga
      421 ccttatgctc agaaaatgag tgaactcatt tcgactctct attcaaatta tagtgaaaac
      481 ctttcttccc ctttcttcga acagcacgaa accgataaag ttcttctggt gagccttgcc
      541 tccaacagag gcctgtgtgg cgccttcaac tccaatatca tcaaacagat aaaatatctc
      601 accgaaactt cttttgaaca acaatatggc ttaaaccatc taaaactcat cagtattgga
      661 aaacacgtaa gcgatatcct catcaacaaa aaataccctg tgttgcaaat caaagataac
      721 cttatagaaa aagtaacctg ggaaggagta aaattactgg ccgacgaatt gatcgaattg
      781 tggacgaaac acgaattcga tcgtatcgtg ttggtttata accagtttat aaatgctgcc
      841 acacaagtcg taactaccga aacttttctc cctctttctt atgatgaaaa taagaaaacc
      901 cgaaacatcg attatatctt tgaaccttca tcggaaacca tcatcgaaga acttatccct
      961 cattacttgc atatccagct ttataaaact tttctcgatt cctttgcttc cgaacaaggt
     1021 gcaagaatga ctgccatgat gatagctacc gataatgctg atgtgatttt gaaggatctt
     1081 accctgtcat ataataaagc ccgacaggct gccattacaa aagaaatcct cgaaatcgtt
     1141 tcaggttcga acgcattgaa caaagtttag gtttaaatgg ttattatttc gataaaatac
     1201 aaaaacatga aaaagaatat cgagcaagct atcatcgaac agatacagaa agaagagcac
     1261 agttcgaggc tttatcttca aatggctata tggtgtgaaa taaatggctt ccctggagcg
     1321 gcagcctttt tatacaaaca ttccgacgaa gaacgtcttc atcagctgaa attcatccat
     1381 tttctgaacg atagagaagg tatggctcta ttgaaacccc tcgagacccc tcaaagcgat
     1441 tacccctcac ttaaagaaat gttcgagaaa attctcgaac acgaaaaatt tatttccgct
     1501 tctattaacc aaatttatgg tctaacccta caagaaaaag attacacaac tgcaagtttc
     1561 attcattggt tcatcaatga gcagatcgaa gaggaaactt tagtgcggac tattctcgac
     1621 aagatgaaac tcgccggcga tggtcagaac aggggcttat acctcattga taaggaactc
     1681 ggagttatgg ccgcttctgg gcactgactc ggctttagaa cctcctcaac aggggcatag
     1741 attttcgtac aataattatt tctactctga caaccgaaag ggctatgcaa agtaagctgc
     1801 taaatggtgg atggcggccg gagagtgcgg gtaggagact aaaaagtgaa aaaccaaacg
     1861 tcacatcttc tcaaaaaata tggctgggga aggcaattgc ggcaacgctg atccttactc
     1921 cctccatctc caataatcta tt
//
LOCUS       DAORPD010000311         1941 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_10921_length_1941_cov_2.283139, whole genome shotgun sequence.
ACCESSION   DAORPD010000311 DAORPD010000000
VERSION     DAORPD010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10921_length_1941_cov_2.283139"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1028)
                     /locus_tag="PK976_04480"
     CDS             complement(<1..1028)
                     /locus_tag="PK976_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67175.1"
                     /translation="MTKAERLFTLKRLGNLLRWITLTGVYFTPSDEEIELSKKLQRII
                     DENHHFNLWFDPFHVKYSLSALGLMLDETRLENWANRYIIPEDRINRKTIAVVCAGNI
                     PAVGFHDFLCVLVSGHNILVKLSSDDLLLLPWFSEFLHNTNPEFKNIITITEGTISGF
                     DAVIATGSNNTSRYFEYYFGKYPHIFRRNMNSIAILDGQESPQELSGLVDDILIYYGR
                     GCRNVSFLLVPENYDFSPLLDAFRKYSHYAYHSKFYNNYEYNKAVMLVNRIPFYDIGF
                     MLLTSHPSIQSPLAVIHYQTYKKSDEIYSFLHFNRDKIQCNVSHIQGIPIKVDFGKSQ
                     QPALDDYA"
     gene            1109..>1941
                     /locus_tag="PK976_04485"
     CDS             1109..>1941
                     /locus_tag="PK976_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67176.1"
                     /translation="MKRTLLYLFLPVLVNLYSNQSIKAQKVHPNQDTLLAYWQIMLRG
                     ESDSVQRVASLKVKEILSQSFTTTQCLSLKLPKEIRLSALMPTDSAFLLITWNIPLST
                     GGYFYDGLLVAKNTPDSKLSVYPLKDKSDEIKNPETLITRPENWYGALYYQLLEFKSE
                     KGKFYVLLGWDGGNGRIQRKIIEVLSFDDDHLPRFGDKIFDQSPKTRLIFEYASGSYF
                     VLRYEKQHYRKKVWYKRKPVIKEEAMIVMNRLVYEPTVGANVAAANILDAYLFSDGKL
                     I"
BASE COUNT          622 a          374 c          388 g          557 t
ORIGIN      
        1 tcagcatagt catccagagc aggctgttgt gattttccaa aatccacttt aatgggaatt
       61 ccctgaatat gactgacatt acattgaatc ttatccctgt taaaatggag gaaagagtat
      121 atctcatccg attttttata tgtttgataa tgaatcactg ccaatggact ttgaatggag
      181 gggtgggaag ttagcagcat gaaacctata tcatagaagg ggatgcgatt caccaacatc
      241 acagccttat tgtattcgta attattataa aacttcgaat gataggcata atgagaatat
      301 ttccgaaagg catcgagcaa aggtgagaaa tcataatttt caggaacgag caggaaggaa
      361 acattacggc acccacggcc ataatagatt aaaatatcat cgacaagacc cgaaagttcc
      421 tgtggcgatt cttgtccatc aagaatggca atgctgttca tattccttcg aaatatgtgg
      481 ggatatttcc caaaataata ttcgaaatac ctggaagtat tgttcgaacc tgtggctatt
      541 accgcatcga agcccgaaat tgtaccctcc gtgatggtga tgatattctt gaattcagga
      601 ttggtgttat gcagaaattc agaaaaccaa ggcaacaaaa ggaggtcgtc ggaggataat
      661 ttcaccagaa tgttatgacc cgatacgagt acacataaaa aatcgtgaaa cccgactgct
      721 ggtatattcc cagcgcacac tacggcaata gttttcctgt tgattctatc ttcgggaata
      781 atatagcggt tagcccaatt ttcgagtctc gtttcgtcga gcatcaatcc aagcgcagaa
      841 agtgaatatt ttacatgaaa aggatcaaac cagagattaa aatgatgatt ttcatcgatt
      901 attctttgca atttctttga taactcgatt tcttcatcag acggagtaaa ataaacacct
      961 gtcagggtaa tccacctcaa cagatttccc aaacgtttca atgtaaataa acgttctgcc
     1021 tttgtcattt ttaaatcttt acttttgcaa agctaagaga ttatgaagta atattttaaa
     1081 ttgtcactcg cctccacatc aagatgatat gaaaagaacc ctattatatc tcttcctgcc
     1141 tgttttggtg aatttatatt caaatcaaag cattaaggct caaaaagtgc acccgaatca
     1201 ggatactttg cttgcatatt ggcagataat gctaaggggc gagagcgaca gtgtgcaaag
     1261 ggttgcatcg ctcaaagtaa aggagattct ttctcaaagt tttaccacaa ctcagtgcct
     1321 ttctctcaaa ctgcccaaag aaataagact ctcggcattg atgcccacag actcagcttt
     1381 tctattgatc acatggaata ttcctctttc gacaggaggt tatttttatg atgggctact
     1441 agtagctaag aatacacccg attcaaaatt atcggtttat ccactgaaag acaaatcgga
     1501 tgagatcaaa aatcccgaaa ctctcataac caggcctgaa aactggtatg gcgctttgta
     1561 ctatcaacta ttggaattca aatcggagaa aggtaaattc tatgtcctgc tgggctggga
     1621 tggaggcaac ggccgcatac aaagaaaaat catcgaggtg ctaagttttg atgatgatca
     1681 cttgcctcga ttcggagata aaattttcga tcaatcaccc aaaactcgtc taatattcga
     1741 atatgcttcg gggagttatt ttgtcctaag atatgaaaaa caacattatc ggaagaaggt
     1801 atggtataaa cgcaaacctg tcatcaaaga agaagccatg attgtgatga atcgtctggt
     1861 atatgaaccg acagtaggag ccaatgtagc cgcggcgaat attctggatg cctatctttt
     1921 ttccgatggg aagcttattt t
//
LOCUS       DAORPD010000312         1941 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_10922_length_1941_cov_1.930011, whole genome shotgun sequence.
ACCESSION   DAORPD010000312 DAORPD010000000
VERSION     DAORPD010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10922_length_1941_cov_1.930011"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="PK976_04490"
     CDS             complement(<1..522)
                     /locus_tag="PK976_04490"
                     /inference="COORDINATES: protein motif:HMM:NF014114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HRC67177.1"
                     /translation="MNHLYRRPAYLNSGDKICITSPAGRVAPEKVLKADKVFKNYGFE
                     VEIMSHALGAFYQFSGTDDERLEDLQQALDDPYCKAILMSRGGYGLHRIIDRLDFRSF
                     ETFPKWIIGFSDITLLHAHLHRLKWQSLHSPMASAFSIENPSQALKVLSFLQGNLPKY
                     SLAPHALNRKGKAR"
     gene            690..>1941
                     /gene="metG"
                     /locus_tag="PK976_04495"
     CDS             690..>1941
                     /gene="metG"
                     /locus_tag="PK976_04495"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HRC67178.1"
                     /translation="MASENNKSYKRTTITSALPYANGPVHIGHLAGVYIPADIYARYL
                     RSKGEEVLFIGGSDEHGVPITIKARKEGISPQQVVDKYHEIIKKSFEELGISFDIYSR
                     TSLPIHHETASNFFKKLYEEGKFIEKVTEQYYDAEVGQFLADRYIIGTCPICGNQGAY
                     GDQCEQCGSSLNATDLIQPRSALSGSIPVLKATRHWFLPLNEYEAWLKIWILEEHKDD
                     WKINVYGQCKSWLDHGLQPRAVTRDLDWGVKVPIQGTEGKVLYVWFDAPIGYISATKH
                     WAQLTGGDWEKWWKSPDTRLIHFIGKDNIVFHCIIFPVMLKAEGSYILPDNVPANEFL
                     NLEGDKISTSRNWAVWLDEYLKDFPGKQDVLRYVLCANAPETKDNDFTWKDFQTRNNS
                     ELVAIFGNFVNRTAVLATKFFDGRI"
BASE COUNT          580 a          395 c          430 g          536 t
ORIGIN      
        1 cctcgcctta cctttacggt tgagggcatg aggtgctaag ctatatttcg gaagatttcc
       61 ctgtaaaaaa ctcaacactt ttaaagcctg acttggattt tctatagaaa atgccgaagc
      121 catgggactg tgcagacttt gccatttcag acgatgcaaa tgagcatgta agagggtgat
      181 atcgctaaat ccaatgatcc atttgggaaa cgtttcgaag gaacgaaaat cgagcctgtc
      241 gatgatacga tgaagcccat atccacctct cgacatgaga atagctttgc agtatggatc
      301 gtccaaggcc tgttgcaaat cttcgaggcg ttcgtcgtcg gtaccgctaa attggtaaaa
      361 tgcacccaaa gcatgggaca tgatttctac ttcgaatcca taattcttga aaactttatc
      421 ggccttcaat accttttcgg gcgcaacacg tcccgctggt gatgtgatgc aaatcttatc
      481 gcctgaattt aaataggcag gccttctata taaatgattc attcgaatga atagaaatgc
      541 taaagtataa atttttcaat cgaccacata tcgtcataaa agatttattt tcggtataga
      601 cataccggaa ttaagggtga ggaaagcgag ttgcaatata ttggcaagca agccgattat
      661 ttatctttgc aactcaaatt ttcgagataa tggcttcaga aaataataaa tcctataaac
      721 gtactaccat tacttcggca ttgccttatg ccaatggtcc tgtgcatatt gggcatttgg
      781 ctggagttta cattccagcg gatatttatg ctcgctatct gcgttcgaaa ggagaagaag
      841 ttttatttat aggtggaagc gatgagcatg gcgtgcccat taccataaaa gccagaaaag
      901 aaggtatcag cccacaacag gtagtagata agtatcacga gattataaaa aaatcattcg
      961 aagaattggg tatatccttc gatatataca gccgcacttc cttacctata catcatgaaa
     1021 cggcatcaaa ttttttcaaa aaattatatg aagaggggaa attcatcgaa aaagttactg
     1081 aacagtatta tgatgcagag gtagggcaat ttttagccga tcgttatatt attggtacct
     1141 gtcccatttg tggcaatcag ggggcttatg gcgatcagtg tgaacagtgc ggaagttcac
     1201 tcaatgctac cgatcttatt caacctcgat cggccttaag cggaagcatc ccggttttga
     1261 aagcaacccg ccactggttt cttccgctca atgagtatga agcatggcta aaaatatgga
     1321 tactcgaaga acataaggat gactggaaaa taaatgttta tgggcaatgc aaatcatggc
     1381 tcgatcatgg attgcaaccg cgggccgtta cccgtgatct cgactgggga gtaaaagtgc
     1441 ccatccaagg taccgaaggc aaagttttgt atgtatggtt tgatgctccc atcggttata
     1501 tctcggctac aaaacactgg gctcagctaa ctggcggaga ctgggaaaaa tggtggaaat
     1561 cgcctgacac acgtttgata catttcatcg gcaaggacaa tatcgttttc cattgcatca
     1621 tctttccggt tatgctgaag gccgaaggaa gctatattct tcccgataat gttcctgcca
     1681 atgaatttct gaatttagag ggcgacaaga tttcaacttc tcgcaactgg gcggtctggc
     1741 tcgacgaata ccttaaagat tttcccggca aacaggatgt gctgcgttat gtgctgtgcg
     1801 ccaatgctcc cgaaaccaaa gacaacgatt ttacatggaa agactttcaa acacgtaata
     1861 atagtgagct tgtggctata ttcggaaatt tcgtcaaccg aacggctgtg ctggcaacaa
     1921 aatttttcga cggacggatt c
//
LOCUS       DAORPD010000313         1937 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_10950_length_1937_cov_2.501063, whole genome shotgun sequence.
ACCESSION   DAORPD010000313 DAORPD010000000
VERSION     DAORPD010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10950_length_1937_cov_2.501063"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            220..624
                     /locus_tag="PK976_04500"
     CDS             220..624
                     /locus_tag="PK976_04500"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HRC67179.1"
                     /translation="MATIETIYLGELRTKATHLQSGSSIITDAPLDNQGRGEAFSPTD
                     LLAAALASCMLTIMGIAARTHGFNLDGTKAEITKIMESNPRRVGEIIIHLYFPPHPFT
                     EKEKQIIRNSAFTCPVYQSLNEQLKKTVHFHF"
     gene            640..1026
                     /locus_tag="PK976_04505"
     CDS             640..1026
                     /locus_tag="PK976_04505"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRC67180.1"
                     /translation="MDIIHVPALPLNFEQKKIILAEDTASHYGSGLLEVFATPAMIAF
                     MEKTCYEGIAHYLAPGYSSVGTKVHVEHLRAIRVGVKIHCEARLNTIDGKKLFFEVRV
                     FDHQGIIGQGTHTRYIIDIEKFMLKL"
     gene            complement(1028..1480)
                     /gene="dtd"
                     /locus_tag="PK976_04510"
     CDS             complement(1028..1480)
                     /gene="dtd"
                     /locus_tag="PK976_04510"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HRC67181.1"
                     /translation="MRVVIQRVSRANVKIDERLISEIGYGLMVLCGFEEGDEVEDLRW
                     IAGKISRLRIFDDEAGMMNLSVVDKAGEMMVISQFTLHASTKKGNRPSYIRALEPVRA
                     KELYQLFVDELVCYSGIGVKTGIFGAYMMIELVNDGPVTIIIDSKMRE"
BASE COUNT          617 a          417 c          318 g          585 t
ORIGIN      
        1 aagcctataa aaccttgcga tcatcctata cagggaaaaa atacctgctg cgacaaaaaa
       61 gagaaaaaag cttcatgcaa cgaaattcat aaggttaagc aggctgaaac aaaaccagct
      121 actcctgaaa agaaataatt ttccgcttgt ttgatggata aacctgaaat atccgggttt
      181 attatttatc ttgcgactca ataaaataat gaaatgctta tggctaccat tgaaaccatt
      241 tatttaggtg agttgcgtac aaaagccaca cacctccagt cgggcagttc gattataacg
      301 gatgccccac tcgacaatca aggcagaggg gaggcattct ctcctactga tttattagcc
      361 gcagccctgg caagctgcat gctgaccatt atgggaattg ccgcccgcac gcatggattt
      421 aatcttgatg gaaccaaagc cgagattact aagattatgg aatcgaatcc acgaagagtc
      481 ggtgaaatta ttattcatct ctattttcca cctcaccctt tcaccgaaaa agaaaaacaa
      541 atcatacgca attcggcttt tacctgccct gtgtatcaga gcctaaacga acaactcaag
      601 aaaaccgtac attttcattt ctaatttgat ttgaaatcta tggacatcat tcatgttccc
      661 gctctacctc tcaatttcga acagaaaaaa atcatactgg cagaagacac tgcttcacat
      721 tatggttcgg gtttgctcga ggtatttgca acacctgcca tgatagcttt tatggaaaaa
      781 acctgttacg aagggattgc tcattattta gctcctggat attcatccgt gggaacaaaa
      841 gtgcatgtcg agcatttaag agccatcaga gttggtgtaa aaattcattg cgaagcccgt
      901 ctaaacacaa tcgacggaaa aaagttgttc ttcgaggttc gagtgttcga ccatcagggc
      961 attataggcc aaggtaccca tacaagatac attatcgaca tcgagaaatt catgttgaaa
     1021 ctttgaatta ctccctcatc ttgctgtcaa taatgatggt aacaggccca tcatttacta
     1081 gttcaatcat catgtaagcg ccaaaaatgc ctgtttttac gccgattccg ctgtaacata
     1141 ccaattcgtc gacgaaaagc tggtataatt ctttggctct cacaggttct aaggctctaa
     1201 tatatgaggg gcgattccct tttttggtac tggcatgcaa agtaaactga cttattacca
     1261 tcatctcacc agctttatct acaaccgaca agttcatcat ccctgcttca tcatcaaaaa
     1321 tcctcaaacg agaaatctta ccagctatcc atctcaagtc ttctacctca tcgccttcct
     1381 caaacccaca taacaccata aggccatagc caatttccga aatcagcctc tcatcaattt
     1441 ttacattggc tctacttacg cgttgaataa ctactctcat gcttaaatga tttgttgatg
     1501 gttctcactt caaagtattt ttacagagct tagcaaagta aattcgcctt tgaatgaaca
     1561 aaattagtat acaattgatt cttattgcct cgaagttctg ttgtaacctc ggtaaaaagt
     1621 tattcaaaga attattctcg gttctatcaa taaagtttct tcattgtgat ttagaaaact
     1681 attttgagtt aaagaggctg taatagtggt gagtaaaccc cttgaaggcc tcaaaaaaat
     1741 ataattgtgt tgtttccttt tttcatgctc aaattccgat taattccaaa aattctctat
     1801 tccaaaaaat tcagtcgttt catgattgga agcaaattta tataaatatc tcagaacaat
     1861 ctttaaaaat aaaatatttt gaatttcaat atttaatatt tattggaatc tttattaaat
     1921 gcactcctca ctccttt
//
LOCUS       DAORPD010000314         1935 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_10969_length_1935_cov_2.634574, whole genome shotgun sequence.
ACCESSION   DAORPD010000314 DAORPD010000000
VERSION     DAORPD010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10969_length_1935_cov_2.634574"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1810
                     /locus_tag="PK976_04515"
     CDS             <1..1810
                     /locus_tag="PK976_04515"
                     /inference="COORDINATES: protein motif:HMM:NF012307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HRC67182.1"
                     /translation="AEKVRKRRQPLIGKSIFSHWLTKKNDSEFFLSDWKYGYYELRKS
                     LKYWYNQGYKWLANFDLAAFYDTIPHELLLRIIAPHRGQTELTDFISNCLQTWSADNR
                     SVQHNHGIPQGPSASDFLAECVMLPIDEKMHQTCVYLRYVDDIRILGKNEYEVRKALV
                     DLDVLCRERGLIPSSEKTTILKIKDEEELVRNIPPILLYQEALGPKQMVEEEAEVAIK
                     EALSLSDNSLEIIDKSKFRYILFRTGPSDNILQMIITLWTQNPHQIDAFASFLENYDR
                     VEDIINLCIQDIDQSPYDFVRGEAWKILARMCTPNECRQLATRAINAVKSQHCPATKI
                     GAYKFLLRCEELGLGSYSKWMMFENQALIQAISINNLELNINKGVEVASQILLRRIPD
                     PSLGLLNPLVKSRMTIDQLGQQPNTLLPVTRNVYFKARIIHDGRRIRLDVLGNKLSQR
                     YKITKWNKWQTLLGSEYQHAYSMLNLAEAYYKGHRSAWLAQQDAFNDALFRAFQLFLA
                     SKSAPGAIPTSNVKGLLDYGALINDNSFKNAYALLSSHLIAVHSRRSTLPNAHPYEKR
                     TGKKAIALKANEQRTMVAHLAAAYAEIIGIVTSLGI"
BASE COUNT          633 a          385 c          370 g          547 t
ORIGIN      
        1 tgcagaaaaa gttcgcaaac gccggcaacc attaatagga aaatcaattt ttagccattg
       61 gctgacaaag aaaaacgact ccgaattttt cttgagtgat tggaaatatg gttattacga
      121 attacggaaa tctttgaaat attggtataa ccaaggctat aagtggttag caaattttga
      181 cctggcagca ttttatgaca ctattccgca tgaattactt ttaagaatta ttgccccaca
      241 tcgtggacaa accgaattaa cggactttat ttccaattgt ctgcaaactt ggagtgcaga
      301 taatcgttca gtccaacata atcatggtat tccacaaggt ccatcagcat cagattttct
      361 agccgaatgt gttatgttgc ctatagacga aaaaatgcat cagacttgtg tttacctccg
      421 ttatgttgat gacatacgga ttctagggaa aaatgaatat gaagttcgta aagcactggt
      481 agatttggat gttttatgta gagagagggg attaatccca agttctgaaa aaaccacaat
      541 tttaaaaata aaagatgaag aagaacttgt ccgaaacatt ccaccaatct tactttacca
      601 ggaagccctc ggtcctaagc agatggtgga agaagaagct gaagtagcca ttaaagaggc
      661 tttgtccttg agcgataact ccttagaaat tattgataaa tctaagtttc gatatatttt
      721 gtttagaaca ggcccttcgg acaatatatt acagatgatt attactcttt ggactcaaaa
      781 cccacaccag attgatgcat ttgcatcttt tttggaaaat tacgatagag tagaggacat
      841 tatcaattta tgtattcaag atattgacca aagcccatat gatttcgtca gaggtgaagc
      901 ctggaaaatc ttggctcgta tgtgtacgcc caatgaatgt cgacaactgg caacaagagc
      961 aatcaatgca gtaaagtcgc aacactgtcc agccaccaaa attggtgctt acaagttcct
     1021 acttcgatgt gaagaattag gattgggttc ttatagtaag tggatgatgt ttgaaaacca
     1081 agccctaatt caggccatat ccataaataa ccttgagctt aatataaaca agggcgtcga
     1141 agtcgcctct caaatcttat tgaggcgaat accggatcca tccttagggc ttctcaaccc
     1201 attagtaaaa tcacgaatga caatagacca gcttgggcaa caacccaata cattgttgcc
     1261 agttactcgc aatgtttatt ttaaagcaag aattattcat gatggtcgca gaattaggtt
     1321 ggatgtctta ggcaataagc tctcacaaag atataagata acaaaatgga ataaatggca
     1381 aacactttta ggaagtgagt atcaacatgc ttacagtatg cttaatttgg cagaagctta
     1441 ttataaaggc catcgatcag cctggcttgc acaacaggat gcgttcaatg atgctctttt
     1501 tcgagctttt caactattct tagcttcgaa aagcgcccct ggggcaattc ccacttccaa
     1561 cgtaaaggga ctattagatt atggagcatt aataaatgat aattctttta aaaatgccta
     1621 tgctcttcta tcgtctcacc taattgcagt tcacagtcga aggagcacac ttcccaatgc
     1681 ccacccatac gaaaagcgca ccgggaaaaa agccatagcg ttgaaggcaa atgaacaacg
     1741 aacaatggta gcacatttag cagctgccta tgcagaaatt attggaatag tgacgagctt
     1801 aggtatttaa tttcccacaa aatattaaga ccttgaggac tgctcaaggt cttaagtaca
     1861 taaatattta tattccaaat cccatctgac cctccggtac tacctccttt ctccttgtcc
     1921 gcctgatcac caccg
//
LOCUS       DAORPD010000315         1930 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_11008_length_1930_cov_2.413333, whole genome shotgun sequence.
ACCESSION   DAORPD010000315 DAORPD010000000
VERSION     DAORPD010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11008_length_1930_cov_2.413333"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            7..>1930
                     /locus_tag="PK976_04520"
     CDS             7..>1930
                     /locus_tag="PK976_04520"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HRC67183.1"
                     /translation="MTKEIPSRYNPETTEDKWYSFWESHGFFHSVPDEREPYCIVIPP
                     PNVTGVLHMGHMLNNTIQDVLIRRARMQGKNTLWVPGTDHASIATEAKVVANLREKGI
                     EKSSLSREEFLKYAWEWKEKHGGIILQQLRKLGASCDWSRTKFTMDPDMYESVIEVFV
                     DLYHKGLIYKGVRMINWDPQALTALSDEEVIYKEVQSKLYYVRYKIYDSEDEWIIIAT
                     TRPETILGDTAVCIHPDDERYKHLHGRKAVIPLINRIVPIITDEYIDREFGTGCLKVT
                     PAHDINDYNLGMKHKLDAIDIFNDDGTLNEKAILYVGEDRLKVRNKITEDLRQKGFLV
                     KEEPYLNRVGFSERTDAMIEPRLSQQWFLDMKPLAQPALDVVMNDTIHFYPQKFKNTY
                     KYWMENVKDWCISRQLWWGHRIPAYYLPNREVIVAKTQKEAYQIALKQYPDLDELKPE
                     DLKQDEDVLDTWFSSWLWPISVFDGIRHPHNPDIEYYYPTNDLVTAPEILFFWVARMI
                     MAGLEYRGEIPFRNVYFTGIVRDKLGRKMSKQLGNSPDPIALMKKYSADGVRVGMLLT
                     SPAGNDLPFDEALCEQGRNFANKIWNALRLLKGWEIDEKIPQPNYSAVACRWFENRFN
                     KSSGGEYLAQGKTVTGF"
BASE COUNT          594 a          407 c          429 g          500 t
ORIGIN      
        1 cttcaaatga caaaagagat tccttcgaga tacaatcctg aaactaccga agataaatgg
       61 tattctttct gggaaagtca tggttttttc cattcggtgc ccgacgagcg tgaaccttat
      121 tgtattgtca tacctcctcc gaatgttacc ggagtattgc acatggggca catgctcaac
      181 aatacaattc aggatgtact aatacgcagg gctcgcatgc aggggaaaaa tacactctgg
      241 gtgcctggta ccgaccatgc atcaatagct accgaagcca aagtggtagc taacctgcga
      301 gaaaaaggaa tcgaaaaatc ttcactttcc cgtgaggagt ttcttaaata tgcttgggaa
      361 tggaaagaaa aacatggagg tatcattctg cagcagcttc gtaaattagg agcttcgtgc
      421 gactggtcga gaacgaaatt taccatggat cccgatatgt acgagtcggt aatcgaggtt
      481 ttcgtcgatt tataccataa aggactcatc tacaaaggcg taaggatgat taactgggat
      541 ccacaagcat taacggcctt gtccgacgaa gaagtcattt acaaggaagt gcaatccaaa
      601 ctctattatg tgagatataa aatttacgat agtgaagacg aatggatcat cattgccacg
      661 acccgacccg agaccatttt gggcgataca gcagtctgca ttcatcccga tgatgaaaga
      721 tacaaacatc ttcacggccg aaaagcagtt ataccgctca tcaacagaat agtacccatt
      781 atcaccgatg aatacatcga cagagaattt ggtacgggtt gtctgaaggt tactcctgct
      841 cacgacatca acgactataa tttaggtatg aaacataagc tcgatgccat tgatatcttc
      901 aatgacgatg gaactttgaa cgaaaaggcc attctttatg tgggtgaaga ccgtttgaag
      961 gttcgtaata aaattactga agacttacgc caaaaaggat ttcttgtgaa agaagaacct
     1021 tatttgaacc gagtcggatt cagcgaaaga acagatgcaa tgatcgagcc acgactttcg
     1081 cagcagtggt tcctcgatat gaaacccctc gcccaaccag cacttgatgt ggtgatgaac
     1141 gataccattc atttctatcc tcagaagttc aaaaacactt ataaatactg gatggaaaat
     1201 gtaaaagact ggtgcatctc gaggcagttg tggtggggcc atcgtattcc tgcctattat
     1261 ttacccaatc gagaagttat tgttgccaaa acacaaaaag aagcctatca aattgccctg
     1321 aaacaatatc ctgatctcga tgaattgaaa ccagaagatc tgaaacagga tgaggacgta
     1381 ctcgacactt ggttttcgtc gtggctttgg cctatctcgg tattcgatgg catacgccat
     1441 cctcataatc ccgacatcga atattattat ccgaccaacg atttggttac ggctccggag
     1501 attctatttt tctgggtagc acgtatgatt atggctggat tggaatatag aggcgaaatt
     1561 ccctttcgta acgtctattt tacaggcatt gttcgcgaca aattaggtag aaaaatgtcg
     1621 aaacaactgg gcaattcgcc tgatcctatc gcactaatga aaaaatacag tgccgatggc
     1681 gttagggtag gaatgttgct tacttcaccc gcaggcaatg atctgccttt cgatgaagca
     1741 ctatgtgaac aaggccgaaa tttcgctaat aaaatctgga atgctttacg cttgttgaaa
     1801 ggttgggaaa tagatgaaaa aattccacag cccaactatt cggccgttgc gtgccgatgg
     1861 ttcgagaata ggttcaacaa atcatccggc ggcgaatacc tcgcacaggg gaaaactgtt
     1921 accggctttg
//
LOCUS       DAORPD010000316         1929 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_11022_length_1929_cov_1.759872, whole genome shotgun sequence.
ACCESSION   DAORPD010000316 DAORPD010000000
VERSION     DAORPD010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11022_length_1929_cov_1.759872"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="PK976_04525"
     CDS             <1..364
                     /locus_tag="PK976_04525"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRC67184.1"
                     /translation="GKKRNNTGKKMRKNFSARSIQQFNVRVYGIYMNATSVLVTDEFC
                     LGMRMTKFPGGGLEFGEGTLDGLRREIKEELHQEPVILKHFYTTDFFQRALLVEPPSQ
                     LLSIYISSIYLSPGKFLL"
     gene            complement(510..785)
                     /locus_tag="PK976_04530"
     CDS             complement(510..785)
                     /locus_tag="PK976_04530"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HRC67185.1"
                     /translation="MVKHYAITLLYESQIEGFRFSMMEKALRLNITGYVQRQLNNSVF
                     IEAEGEKTNLEKFLEWCKKGPLGVNILRVEVTEGEVKGYSTFDIQHR"
     gene            complement(976..1545)
                     /locus_tag="PK976_04535"
     CDS             complement(976..1545)
                     /locus_tag="PK976_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67186.1"
                     /translation="MQDLEPYKSWHYLYNAARDIRSPFFGRSYHKTLCTHTIYNYYIH
                     PQWDEIGSPTLYIKILFADYIRRFCVIEMLGEWNDCINNDIMYLKRNVVDALTSEGIR
                     YFILIGENVLNYHSDTDDYYQEWFDDIEDGYIIGLNFREHVVQEFIHARLDNYMLFGG
                     RFSEIPWRKFSPQHLFDLTKKMITRRLSE"
     gene            complement(1606..>1929)
                     /locus_tag="PK976_04540"
     CDS             complement(1606..>1929)
                     /locus_tag="PK976_04540"
                     /inference="COORDINATES: protein motif:HMM:NF018201.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67187.1"
                     /translation="IQHNSNEIDARTVTKFANQKGLEIRYIHQMNLAEGIFSAVEGGD
                     GGHCEQCNRLRLTADGFIKPCLFSDLRYEVRQLGARNALLETIRNKPKEGTTNRINYF
                     YNIGG"
BASE COUNT          551 a          379 c          376 g          623 t
ORIGIN      
        1 cgggaaaaag agaaataaca ctggcaagaa aatgaggaag aatttttctg ctcgatctat
       61 acagcagttt aacgtgaggg tgtatggaat ttacatgaat gccacttctg ttttggttac
      121 cgacgaattt tgcctcggca tgaggatgac taagtttccg ggtggtgggc tcgaatttgg
      181 tgaaggaacc ctcgatggcc tccgccgcga aattaaagaa gaactccatc aggagcctgt
      241 cattctaaag catttttata ctaccgattt cttccagcgt gctttgctgg tagaaccgcc
      301 ttcacagctt ctttccattt atatctcatc gatttacctt tcccccggaa aatttctctt
      361 gtagataaac cttttgattt ttctgttgaa caagagggag cccaaacttt cagaatgcta
      421 aacctgcatg agtctaacga ggtgatgact tttcctatag atcgtcatgt ttggaaactc
      481 ttgcagaaaa cctttttgca agaattacct tagcgatgtt gaatatcgaa agtgctgtag
      541 cctttcactt ctccttcggt aacttctacc cttagaatgt tgactcccag tggtcctttt
      601 ttacaccatt ctaaaaattt ttccagattg gttttttctc cttcagcttc gataaataca
      661 ctgttattca gttggcgttg tacataaccg gtaatattca atcgcagggc tttttccatc
      721 atggaaaaac gaaaaccttc tatttgactc tcataaagca aggtgatggc gtaatgttta
      781 accataagat gaaaatgaat taaattaaaa ttctgataat acaaaggtac gaattatgat
      841 tcgaaaattc tatactgaag ctgaggattg taagattttt ttacagtatt gcccttacat
      901 gttaggtgtt gtataatgcg ttgaatttta gagaatggga tttagggaat gaagaatagg
      961 agttaaaagc gtttttcatt cactcagtct tcgtgtaatc attttcttgg ttaaatcgaa
     1021 caaatgctga ggcgagaact tccgccaggg tatttcactg aaccgaccgc caaaaagcat
     1081 gtaattatcg agccgggcat ggataaattc ctgaacaacg tgctcacgaa aattcagtcc
     1141 aatgatataa ccatcttcaa tgtcatcgaa ccactcctga taataatcgt cagtatcgct
     1201 atgataattg agcacatttt ctcctatcaa aataaaatac ctgattcctt cggatgtgag
     1261 tgcgtcgacc acgttccgtt tcaaatacat gatatcgttg ttgatgcaat cgttccattc
     1321 gcccaacatt tcgattacac aaaatcttct gatataatcg gcaaataaaa ttttgatgta
     1381 gagtgtgggt gagccaattt catcccattg tggatgtata taataattgt aaatagtatg
     1441 ggtacataat gtcttgtgat agcttctccc aaaaaacggg cttcgtatgt cgcgcgctgc
     1501 attataaaga taatgccaac ttttgtaagg ttctaagtcc tgcattctaa aactcctcga
     1561 aaatatattc taaatgcgat tttaaacaag atttgtgcct tgagtttatc ctccaatatt
     1621 gtaaaaataa tttattcgat tggttgtacc ttcttttggt ttgttgcgga tggtttccaa
     1681 aagagcattg cgtgcgccta attgtctaac ctcgtaacgt aagtcggaaa acaggcaagg
     1741 ttttatgaaa ccatcggcgg taagccgaag gcggttacat tgctcgcaat gccctccatc
     1801 gcctccctcg acggccgaga agattccttc cgccagattc atttgatgaa tataacgaat
     1861 ttctaagcct ttttgatttg caaattttgt aaccgttcgg gcatcaattt cgttgctgtt
     1921 gtgttgtat
//
LOCUS       DAORPD010000317         1923 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_11079_length_1923_cov_2.573876, whole genome shotgun sequence.
ACCESSION   DAORPD010000317 DAORPD010000000
VERSION     DAORPD010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11079_length_1923_cov_2.573876"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1088
                     /locus_tag="PK976_04545"
     CDS             <1..1088
                     /locus_tag="PK976_04545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRC67188.1"
                     /translation="LSKYHQITLIALTDQKVHPQAKDKLLNFCDEVYFLKLNKLGILL
                     NVIGAFFRGWPLQIGYFYNKKVKKEIDSIVRQVKPNHIYCQLIRTAEYIHDFDIPKTI
                     DFQDTFSIGMKRRYEKANFLLKPFFHLEYKRLMRYERRIFDLFHNHTLISCPDKELFP
                     HPNKYKIFIIENGVDFEYFETKSPIKKYEVIFTGNMGYPPNIDAAFYLANEIMPLVRK
                     QFPQAHLVLAGATPHPKIKRLANTYTIVTGWVDDIREWYDTSMIFIAPMRLGTGLQNK
                     LLEAMAMHLPCITSSLANEALHAIPNEEILIGDSAEEYANHIIDLLVNPALYNKLILN
                     GHRFVIENYSWENASEKLNAILTHQKD"
     gene            complement(1218..1763)
                     /locus_tag="PK976_04550"
     CDS             complement(1218..1763)
                     /locus_tag="PK976_04550"
                     /inference="COORDINATES: protein motif:HMM:NF025268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HRC67189.1"
                     /translation="MKSAKLRLVLFVVLLTTTIAFQPEEKSTVQWQTFESAIAKNEGK
                     IKKKVFIDVYTDWCGWCKKMDATTFSEKAVASYMNEHFYCGKLNAERCDSIRYNGKIY
                     VNTCPSSKKSAHQLAIALLQGRMYYPSYVILDENNQKLTSFEGYLKPEEMMPILVYFA
                     ENHYLKMSWENFKKEYDDHKK"
BASE COUNT          626 a          401 c          300 g          596 t
ORIGIN      
        1 gcctttcgaa atatcatcaa attaccctca tagctcttac cgaccaaaag gtccatcctc
       61 aggcaaaaga taagctgctg aacttctgtg atgaagttta tttcttgaaa ttaaataaat
      121 taggaatact acttaatgtc attggcgcct tttttcgcgg atggccattg caaataggtt
      181 atttctataa taaaaaagta aagaaggaaa ttgattccat cgtccgtcaa gtaaaaccca
      241 accatattta ttgtcaactg attcgaactg cagaatacat ccatgacttt gatattccta
      301 aaaccattga cttccaggat actttttcaa taggcatgaa acgtcgttac gaaaaggcca
      361 attttcttct caaaccattc ttccatcttg aatacaaacg tctgatgcgt tacgaacgaa
      421 gaatattcga cttgtttcat aatcacactc tcatttcctg ccccgacaaa gagcttttcc
      481 ctcaccctaa caaatataaa atttttatca tcgaaaatgg agtcgatttc gagtatttcg
      541 aaacaaaatc tccaattaag aaatacgaag taatctttac aggaaatatg ggatatccgc
      601 caaatatcga cgcagcattt tatttagcca acgagattat gccccttgtg agaaaacaat
      661 tcccccaggc tcatttagtt ctggctggag cgacacccca tccaaaaatc aaaagattag
      721 ccaacactta taccattgta acaggatggg ttgatgatat ccgtgaatgg tacgacactt
      781 ctatgatttt tatagctccc atgcgccttg gaacaggctt acaaaataaa cttctcgaag
      841 ctatggccat gcatctaccc tgtattacct cttcactggc caatgaggcc ttgcatgcaa
      901 ttcccaatga agaaatcctg ataggagatt cggccgaaga gtatgccaat catatcatag
      961 atttactcgt aaaccctgca ctctataata aattaatcct caatggacat cgattcgtaa
     1021 tcgagaatta cagttgggaa aatgcctcgg aaaaattgaa tgccatactc actcatcaaa
     1081 aggattgaaa agtcaaataa ttacttataa ctccctcctt ctctcacata aatatttctt
     1141 ttcccgtaaa aaattcgaat aaaccataag aattctggaa tagttattat aaacgattga
     1201 tacacgttct cagaggttta cttcttatgg tcatcgtatt cctttttaaa attttcccac
     1261 gacatcttaa gataatggtt ttcggcaaaa tatacgagta ttggcatcat ctcttcgggt
     1321 tttaggtatc cttcaaaaga agtcaatttc tgattgtttt catccagtat cacataagaa
     1381 ggataataca tacgaccctg aagtaaggca atagctaact ggtgtgctga ttttttgcta
     1441 gatggacaag tattcacgta aatttttccg ttatatctaa tcgaatcaca tcgttcggca
     1501 ttcaattttc cacaatagaa gtgttcattc atgtaagatg ctacggcttt ttcggaaaag
     1561 gtagtagcat ccattttttt acaccaacca caccaatcgg tgtatacatc gataaatacc
     1621 tttttcttta tctttccttc gttttttgca atggccgatt cgaatgtctg ccattgaaca
     1681 gtggattttt cttcaggctg aaatgctatc gttgttgtaa gtaacacaac aaacagaaca
     1741 agtcttaatt ttgcagattt catgaatgaa tccatttggt tgtacaaaaa taaatcatac
     1801 atgaatcata atggcaaacc ccgtccagtt atgcatcata tttgaaacat aaaactaccc
     1861 gcttacatat cggataaaaa gaatgatatg cccgaattaa cttacgaatt tgtaaaatct
     1921 ctc
//
LOCUS       DAORPD010000318         1922 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_11092_length_1922_cov_2.475094, whole genome shotgun sequence.
ACCESSION   DAORPD010000318 DAORPD010000000
VERSION     DAORPD010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11092_length_1922_cov_2.475094"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..481)
                     /locus_tag="PK976_04555"
     CDS             complement(2..481)
                     /locus_tag="PK976_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67190.1"
                     /translation="MMKILGCGLLNLAFFAVPIITLAQPPQDNPDKWEQIKAQKIAYL
                     SAKLELKPSESQKFWPVYNEYSEKRDKLLNEIYPKLSKQELDRLMMLSDKEAAQRMDE
                     YFIKQTQLLDLEKKYNACFRKILPDSKVFRLYMAEMMFKKELLDDMRRSKHGPKEEY"
     gene            complement(501..1022)
                     /locus_tag="PK976_04560"
     CDS             complement(501..1022)
                     /locus_tag="PK976_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67191.1"
                     /translation="MKTKKEIDREISEKRLEARFGKKNSFPVPEGYFADLPQNIIQKI
                     KSEQTFTLSFDHSPRTQRRWLIPAITIAVAAFIGAFFLFKPLTTQVNSIDKQVVSKGD
                     IQNDASDYYYISHILYNLPYDHLLNAINKEDDTYLDSVIYSRSLIPDGTLKEEEVIDY
                     LYYHMSIYDITQF"
     gene            complement(1072..1644)
                     /locus_tag="PK976_04565"
     CDS             complement(1072..1644)
                     /locus_tag="PK976_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005831992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRC67192.1"
                     /translation="MTLKIEVVLPKEDDKRLLNLLAQEKTKNYGFELLVKTYQERIYS
                     IVRRMVIRHEDANDIVQDVFVKVWQNLDNFRQESSLYTWIYRIAVNESLRHLKSRKVK
                     IFSSDVDVQKHLEQLIDNPLHFNGDQLEAKLQKAILKLPPKQRLVFNMKYYEDLTYEQ
                     MSAILGTSVGALKASYHLAIKKLEHYLDIY"
BASE COUNT          557 a          353 c          346 g          666 t
ORIGIN      
        1 gctaatattc ttctttcggc ccgtgtttcg agcggcgcat atcgtccaat aattcttttt
       61 tgaacatcat ttcggccata taaagcctaa acactttact atcgggtaaa atcttgcgaa
      121 aacaagcatt gtatttcttt tccagatcaa gcaattgcgt ttgtttaatg aagtattcat
      181 ccattctttg agcagcttcc ttatcggaaa gcatcatgag tctgtctaat tcctgtttcg
      241 aaagtttagg ataaatttca ttgagcaatt tatcacgttt ttcggagtat tcattataaa
      301 cgggccagaa tttctggctt tcggaaggct tcagctcgag ctttgcactg aggtaagcaa
      361 ttttttgggc ttttatttgt tcccatttgt cggggttatc ttgaggaggc tgagctaagg
      421 taataatagg tactgcaaag aatgctaagt tcagcaatcc acatcccagt attttcatca
      481 ttctttgagc tttcatagtt ttaaaattgt gttatatcat aaatcgacat gtgataatac
      541 aaataatcta taacctcctc ttctttgagc gttccgtcgg ggatcaagct tctcgaatag
      601 atgacgctgt cgaggtaagt atcatcttct ttatttatag cattcaataa atggtcataa
      661 ggtaaattat acaaaatgtg agaaatgtaa tagtagtcag aagcatcgtt ttgaatatct
      721 cccttcgaaa ccacttgttt gtcgattgag tttacctgag tagtaagggg cttgaataag
      781 aagaaagctc cgataaaagc agccacagct atagtaatgg ctgggatcaa ccatcttctt
      841 tgagtacgag gggaatgatc gaaagaaagg gtaaaagttt gttccgattt aattttctgt
      901 atgatgtttt ggggaaggtc ggcaaagtaa ccttcaggaa caggaaaaga attttttttc
      961 ccaaaacgag cctcaagccg tttttcagaa atttccctat cgatttcctt cttggttttc
     1021 atttcttgta ccctcttctc agtttgtttg acaataatac agaaaaaagg tttaatatat
     1081 atccagataa tgttctaatt tcttaatggc cagatgataa ctcgccttga gggcaccaac
     1141 cgaagtaccc aatattgccg acatttgttc gtaagtcaaa tcttcataat atttcatatt
     1201 aaaaaccaaa cgttgttttg gtggtagttt tagaatggcc ttctgaagct tggcctcgag
     1261 ttgatcgcca ttaaaatgta atggattatc aatcagttgc tcgaggtgtt tctggacatc
     1321 cacatcggac gaaaaaatct tgactttcct gctttttaag tgcctcaatg attcattcac
     1381 cgcaatacgg taaatccatg tatataaaga tgattcctgt ctgaagttgt ccaaattttg
     1441 ccaaaccttt acgaaaacat cctgaacaat atcattggca tcttcatgtc ttatgaccat
     1501 gcgtctgacg atagagtata tccgttcctg ataggttttt actaagagct caaaaccata
     1561 atttttggtt ttttcctgag ctaacagatt caaaagtcgt ttatcatcct cctttggcaa
     1621 aactacttct atttttaagg tcaaatttga ttatttcatc cttcttgata cttattttca
     1681 aaacggccaa attacggtaa aagtatgcaa caaaactaat tcattacttt tgccgaggtt
     1741 gaaattattc aatctgcttc ttatgaaaaa taattcgatc aataaagttt tttcaaagat
     1801 cattctattt tttttgcttt taatatcgtt ccagtcagag agtaaagatt tctcctcgga
     1861 atttgtcttg cagcacgatt ctcttagatg gattgattca gtgtatagga gcttgacaac
     1921 cg
//
LOCUS       DAORPD010000319         1921 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_11108_length_1921_cov_2.017149, whole genome shotgun sequence.
ACCESSION   DAORPD010000319 DAORPD010000000
VERSION     DAORPD010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11108_length_1921_cov_2.017149"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1921
                     /locus_tag="PK976_04570"
     CDS             <1..>1921
                     /locus_tag="PK976_04570"
                     /inference="COORDINATES: protein motif:HMM:NF013957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HRC67193.1"
                     /translation="EFFSNEPIALTHSLEEFQPDHPDFFSPANSFIKVNLDKIQGTGF
                     KKLALQIYRQLAALHLTYQEFPPLIYNDLERIEFVYQNTGLSDKDSLYLQSLINLKNV
                     YSENPHSTLVDHRLALWYYDKDELLKAANICRNAIEKYPETLGAKNCASLLNELYKPS
                     LQIYCQNTFPPHSPILTQFFYKNTDSIFYRIYRYDERKAGSRGMRQEDLFKNLLSKKY
                     LRNIGVKVIDPGDLKVHSTEIIIEGLENGSYFLLASTGKNFDTIRDILVVQSLRISGL
                     NAATSTLPDRRSVVYVVDRIQGFPVADAAIKLFEQYYKPSTRTWEKHLAAETKTNLQG
                     MAFFPLIRHSELKQPSCLIIFQSDTLYLENFFPQNIYNPVKTPIKRVYLFTDRKIYRP
                     GQTLYYKGIFTETTGKQYQLLTNEQCNVQLFDANGKQIEQKAHKTNRFGSFSGRMTLP
                     SAGLNGSYSLVTPYGSVVFLVEEYKRPNFEVSFESPSEVYKLGEKVSLRGKAIAYSGF
                     PIGQAKVSYRVTRSAFSPYPRSKWWPPLIPSEETEICNMTTTTDEKGFFNVDFTAYPD
                     PSSANDPKIAYRFFIEATIVDLNNETHEAGTSLSIGNRALISYVEVPSQVDKNQPVNF
                     KIISTNLSGKPVNSRG"
BASE COUNT          602 a          388 c          375 g          556 t
ORIGIN      
        1 tggaattttt tagcaatgaa cccattgctc tcactcactc ccttgaggaa tttcaacccg
       61 atcatcctga ttttttttct ccagccaatt ctttcattaa ggtaaactta gacaaaattc
      121 aaggaacagg attcaaaaag ctcgccttgc agatttatcg gcaacttgct gcattgcacc
      181 tgacttatca agagtttcct ccattgatat acaacgatct ggagagaata gaatttgtgt
      241 atcaaaatac gggattgagc gataaagaca gtctttatct acagtccttg ataaatctca
      301 aaaacgtcta ttccgaaaat cctcattcta ccttggtcga tcatcgtttg gctctatggt
      361 attacgacaa ggatgaattg ttgaaagctg ctaatatatg tcgcaatgcc atagaaaaat
      421 accctgaaac cttaggagca aaaaattgtg cttctttgtt aaatgagctt tataagccaa
      481 gtcttcagat ttattgtcaa aatacttttc cgccacattc gcctattctc actcaatttt
      541 tttacaaaaa cacggattct attttttatc gaatttatcg ctatgatgaa agaaaggcag
      601 gttcgagagg gatgagacaa gaagatttat tcaagaattt actctctaag aaatacttgc
      661 gaaatattgg agttaaggta atcgatccag gagatttaaa ggtacattcg accgaaatta
      721 ttatagaagg cctcgagaat ggaagctatt ttcttttggc cagtacggga aaaaatttcg
      781 ataccatcag ggatattctc gtcgttcagt cattacgtat aagtggttta aatgcagcca
      841 caagcacttt gcccgacagg cgttcagtgg tttatgtagt cgatagaata cagggctttc
      901 cggttgctga tgctgcgata aaattattcg aacagtatta taagccttct acaagaacat
      961 gggaaaaaca cttagcggct gaaacaaaga ctaatttgca gggcatggct tttttcccgc
     1021 tcatacggca tagtgagctc aaacagccca gctgtctgat aatttttcaa agcgatacgc
     1081 tgtatctcga gaattttttc cctcaaaata tttataatcc ggtgaaaaca cccatcaagc
     1141 gagtttacct ttttaccgac agaaaaatat atagaccagg gcaaaccctt tactataaag
     1201 gaatttttac ggaaacgacg ggaaaacaat accaacttct gacgaatgaa caatgcaatg
     1261 tgcaattatt tgatgccaat gggaaacaaa ttgagcaaaa agcccacaag accaatcgtt
     1321 ttggttcttt cagcggaaga atgaccttac cttcggccgg attgaatggt tcatattctc
     1381 ttgtaacacc ttatggaagc gttgtttttt tggtggaaga atataaacgg ccaaattttg
     1441 aagtgagttt cgaatccccc agtgaagttt acaagttagg agagaaagtg agcctgagag
     1501 gtaaagccat tgcctacagt ggcttcccaa ttgggcaagc taaggtaagc tacagagtaa
     1561 caaggtctgc cttctctcct tatccacgct cgaaatggtg gcctccgctg attccatccg
     1621 aagaaacgga gatttgcaat atgaccacaa caacagatga aaagggattc ttcaatgttg
     1681 attttactgc ttatcccgat ccttcatcgg caaatgatcc taaaatagct tatcgcttct
     1741 ttattgaagc tactatagta gaccttaaca acgagacaca cgaagctgga acctcgcttt
     1801 cgatagggaa tcgagcattg atatcctatg tggaggttcc ctctcaggtt gataaaaatc
     1861 agccggtcaa ctttaaaatt atatcaacaa atctttccgg gaaaccggtg aattcgcgcg
     1921 g
//
LOCUS       DAORPD010000320         1919 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_11127_length_1919_cov_2.104077, whole genome shotgun sequence.
ACCESSION   DAORPD010000320 DAORPD010000000
VERSION     DAORPD010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11127_length_1919_cov_2.104077"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="PK976_04575"
     CDS             <1..714
                     /locus_tag="PK976_04575"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HRC67194.1"
                     /translation="SEPYSPIITHTTHDEGARRLAALAKIDPTDFEGGMALASDEVTA
                     STHAGTHVDAPWHYGPTCEGKPSKTIDQVPLEWCYGNGVVLDFRYLEQGSEITVEDVK
                     EALNKINYTLNEGDIVLIETGADKYWGTNKYLPAQSGLGMDATEFILNQGVRVIGIDG
                     WGLDRPVAKMSEAYHSTHNKKALWPSHFFGRQREYLQVEKMANLDKLPSTGFKVCVFP
                     IKIERGSAGWTRAVAILED"
     gene            733..>1919
                     /locus_tag="PK976_04580"
     CDS             733..>1919
                     /locus_tag="PK976_04580"
                     /inference="COORDINATES: protein motif:HMM:NF012885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67195.1"
                     /translation="MEEKLFHNEYYWTSPYNYIDEVRAKFQLPEKIKFHDVTLRDGEQ
                     SPGVAFRAEDKVAIAKLLDELGVDRIEVALPAVSQEDLKATKNIVALGLNAKIFVLSR
                     GIASDVEAALDCGVDGIILELPVGIPRLKYQFPTWTEDDVVNKAIQWAKFAKSNGLEV
                     VLFPMDCTRSNPEFFARFLREAGALPEIDGVCLVDTSGSLTPQAAVWLVKKMKEITHK
                     RIEVHTHTDFGMGVATSLAAITAGAEVIHASIAGLGERSGNTPLDEAAISVKALYGLE
                     CNIKYEKLLEVSTKVLELSKFQIAPSKPIIGERAFTRESGMGVDLVKKQPLALFGINP
                     TWVGQKPKYVLGKKSGLASITMKLEDLGMPDLPDEKKLEILNKVKMLSLKKKGLVNDD
                     EFI"
BASE COUNT          595 a          346 c          452 g          526 t
ORIGIN      
        1 agtgaaccat attcaccgat aatcacccac acaacgcatg atgaaggggc aaggcgtttg
       61 gcagcattag caaaaatcga tccgactgat tttgaaggtg gaatggcatt agcatccgat
      121 gaagttactg ccagtacgca tgcgggtaca catgttgacg caccttggca ctacggaccc
      181 acatgtgaag gaaaaccttc gaaaacgatt gatcaagtac ctttagagtg gtgttatggc
      241 aatggagtcg ttttagattt tcgctatttg gagcaagggt ctgaaattac agttgaggat
      301 gtgaaagaag cattaaataa gataaattat acactaaatg aaggtgatat tgttttgata
      361 gaaacaggtg ctgataaata ttggggaaca aataaatatc tacctgcgca gtctggactt
      421 ggcatggatg cgacagaatt tattcttaat caaggcgtta gagtcatcgg aatcgatggc
      481 tgggggctcg accggcctgt agctaagatg tctgaagctt atcactctac acataataaa
      541 aaagcactct ggccttcgca cttttttggt cgccagcgcg agtacttaca ggtcgaaaaa
      601 atggctaacc ttgataagct tccatcgaca ggctttaagg tatgtgtttt tccaataaaa
      661 atcgaacgtg gttcagcagg ttggacgcgt gcagttgcca ttcttgaaga ttagacagaa
      721 agcgtagaaa acatggaaga aaaattattt cataatgaat actattggac aagtccttat
      781 aactatattg acgaagttcg ggctaaattt caattaccgg aaaaaataaa gtttcatgat
      841 gttactctac gggatggaga gcaaagccca ggagtagcat ttcgtgctga agataaagta
      901 gctattgcaa aattattaga cgagcttggt gtagatcgca tagaagttgc cttgcctgct
      961 gtttctcagg aagaccttaa agcaacgaaa aatatcgttg ccttaggcct taatgcgaaa
     1021 atttttgtac tcagccgagg tattgcctcg gatgtcgaag ctgctttaga ttgcggagtt
     1081 gatgggatta ttcttgaatt gccagtggga attcctcgcc taaaatacca atttccaaca
     1141 tggactgaag acgatgttgt aaataaagcc attcaatggg caaaatttgc gaagagcaac
     1201 ggcttggaag tagtgctttt tcctatggat tgcactcggt ctaatccaga attctttgcg
     1261 cggtttttac gtgaagcggg agcattacca gaaatcgatg gagtttgctt agttgatacc
     1321 tctggttccc ttacaccaca agctgcagtc tggcttgtca aaaaaatgaa agagattact
     1381 cacaaacgca tcgaagttca tacgcatacc gattttggaa tgggtgtagc aacttcattg
     1441 gctgcgatta cagccggagc ggaagtaatt catgcttcaa ttgctggctt aggtgagcgc
     1501 tcaggtaaca cacctttgga cgaggcggct atatctgtca aggcgcttta tggtttagag
     1561 tgcaatatta aatatgaaaa actgcttgaa gtatccacta aagttcttga attaagcaaa
     1621 tttcaaattg ctccatcgaa accgataatc ggagaacgag cctttacccg tgaatctggt
     1681 atgggggttg acctggtcaa aaaacaaccc ttagcgttgt ttggaataaa tccaacttgg
     1741 gttggtcaaa aaccgaaata tgtcttaggc aagaaatcgg ggttggcatc aattactatg
     1801 aagcttgaag atttagggat gccggatttg ccagatgaga aaaaattaga aatattaaat
     1861 aaagtcaaaa tgctttcctt gaagaaaaaa ggccttgtca atgacgatga gtttatcga
//
LOCUS       DAORPD010000321         1917 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_11137_length_1917_cov_2.212675, whole genome shotgun sequence.
ACCESSION   DAORPD010000321 DAORPD010000000
VERSION     DAORPD010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11137_length_1917_cov_2.212675"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1552)
                     /locus_tag="PK976_04585"
     CDS             complement(<1..1552)
                     /locus_tag="PK976_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67196.1"
                     /translation="MATVVGKLELCRIDDFEKYFKAIQGIEGHTLFSRFHSLANIVRS
                     NIEVRYQDFLAYPVLDQHGITFYGRKYTETPRLFSELQDEDLEKYNAIKDSTIAHYQD
                     KISSLRNSGNKTEADYLTGAIKFIDNRFLYCYDESVVLGVWGMQLRDTIREDINQIRK
                     SLFTKRKSHPDGSDGIPQPEPTTLEYVVSFNAGENGTLSGESHISKEEHSFLTEDEIP
                     KVEPKEGFHFAGWDENPTDYKVSGDKVFTAQYEGHGTLPPPSVKLPWYRRFWNWLRAL
                     FLDPGCLKWLLWILLFLFLFWLFSALIRGCNEKHHIGGSALNERDSAWIRQDPNVGNV
                     GGIYDPHNPYQLQPTPPGFDNLLPPHQGVLPPITENPDTIPGNPTIIGDRLNVLMENE
                     DKSILDFVKSFKSKYPGEKYKIVYYDDVVKRIQIEVPGSERERLKTEIPGAFKPEYEL
                     FVFDEALFEGTQVSKDPYLSDSDKSWYLSAIHAQQAWSITRGSDKITVAIVDNGFSLD
                     HPELASKVV"
     gene            complement(1557..>1917)
                     /locus_tag="PK976_04590"
     CDS             complement(1557..>1917)
                     /locus_tag="PK976_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67197.1"
                     /translation="SAEMLNRCLNTVGFDYYEESEVNDLRIANEKNRLGLVIEQQNSP
                     MERTVEDLFIKIDNLPELIQSNPEEMKTLPSYRNYLSWYHRLKMGFIYVCDIPSYNVL
                     ANEKLGTLIKECKRITY"
BASE COUNT          529 a          455 c          353 g          580 t
ORIGIN      
        1 ccactacttt gcttgccaat tcggggtgat cgaggctaaa gccattatct actattgcca
       61 cggttatctt atccgaccca cgagtaatac tccatgcttg ttgagcatgg atggcactca
      121 aataccatga tttatcacta tccgataaat aaggatcctt tgaaacctgg gttccttcaa
      181 agagagcttc atcaaaaaca aaaagctcat attccggttt gaaggcccct ggtatctctg
      241 tttttaaacg ctccctctcg cttccaggta cttcaatttg gattctctta accacatcat
      301 cataatacac aatcttgtac ttttcaccag gatacttact cttgaatgat tttacaaaat
      361 cgagtataga tttatcctca ttctccatca acacattcaa gcggtcacca ataattgtgg
      421 gatttcccgg aatcgtatca gggttttcgg tgattggggg cagtacgccc tgatgaggtg
      481 gcaacaaatt atcaaaaccc gggggcgtag gttggagttg gtaagggtta tgcggatcat
      541 aaattcctcc gacatttccc acattcgggt cctgtctgat ccatgcacta tccctctcat
      601 taagcgccga acctccgata tgatgctttt cattacaacc gcgaataagc gctgaaaaaa
      661 gccagaacaa gaataaaaaa agcaaaatcc aaagcaacca cttcaaacag cctggatcta
      721 aaaataatgc gcggagccaa ttccagaatc gcctgtacca cggcaatttc actgagggtg
      781 gaggtaaagt accatgaccc tcgtattgcg ccgtgaacac cttgtccccg gataccttat
      841 aatctgtcgg attttcatcc catccggcaa aatgaaatcc ctccttgggc tccacttttg
      901 gaatctcatc ctctgtcaag aaactatgct cttctttact aatatgggat tctccactca
      961 aagttccgtt ttctcctgca ttgaatgaaa cgacatactc gagggttgtc ggttccggct
     1021 gcgggatccc atcggatcca tctgggtggg atttacgttt cgtaaagaga ctcttccgaa
     1081 tctgatttat atcctcgcgt attgtgtcgc gcaattgcat gccccagaca ccaagaacaa
     1141 cgctttcatc gtaacaatat aaaaatcggt tatctataaa tttgattgct ccggtgagat
     1201 aatccgcttc tgttttattg ccggaattcc ttagcgatga gatcttgtct tggtaatgag
     1261 caatggtgct atccttgatc gcattatact tttccagatc ctcatcctgc aattcagaaa
     1321 ataaccgcgg ggtttcagtg tatttccttc cataaaatgt aatgccatgc tgatcgagaa
     1381 caggatatgc taggaagtcc tgatatctaa cttcaatatt acttctaacg atattggcaa
     1441 gtgaatgaaa tcgactaaac agagtatggc cctctatacc ttgaatggcc ttgaagtact
     1501 tttcaaagtc atcaattcta catagttcta atttgccaac aacagtcgcc attaactcaa
     1561 taggttattc tcttacattc cttaataagt gttcccaatt tttcgttagc cagtacatta
     1621 taactgggta tgtcacaaac gtatataaaa cccattttca gccgatggta ccaggagaga
     1681 taattccgat aactcggcag ggttttcatt tcctcggggt tcgattgaat gagttccggc
     1741 aaattatcaa ttttgataaa caaatcctcc actgtccttt ccataggact gttttgctgt
     1801 tctataacca atcctaatcg atttttctca tttgcaatcc gaagatcatt gacctcggac
     1861 tcttcgtaat agtcaaatcc tactgtattg aggcatctgt tcaacatttc tgcgctc
//
LOCUS       DAORPD010000322         1915 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_11162_length_1915_cov_2.588710, whole genome shotgun sequence.
ACCESSION   DAORPD010000322 DAORPD010000000
VERSION     DAORPD010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11162_length_1915_cov_2.588710"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1037
                     /locus_tag="PK976_04595"
     CDS             <1..1037
                     /locus_tag="PK976_04595"
                     /inference="COORDINATES: protein motif:HMM:NF014818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67198.1"
                     /translation="KKCQVEIPDYESQIVFIDGFPIKILNKERSPQNINWRNEKVRIV
                     VDCTGKFLDPTISPAEGKDALCGHLTAGAQKVISSAPFKIKNASTHDLDSFPTLIYGI
                     NHTSYDPLRHHIISAASCTTTGLAHMIKPLLENKETSNVLTASMSTVHAATNTQSVLD
                     SVPAAGATDLRKNRSVFNNIILSTTGAAKTLEMVIPQVTNFGFMADSIRIPTNTVSLI
                     ALNITFSSSLDEKGMPSINANSIKEIYKTAANGNQKGLLVFSTKQNVSADLLGFAAAI
                     VIEGNDIHTRTGFLKIPQDAISSLGIPDIHEINIPVTHAKIMGWYDNEFGSYVNCLGK
                     LTEHIASRME"
     gene            complement(1703..>1915)
                     /locus_tag="PK976_04600"
     CDS             complement(1703..>1915)
                     /locus_tag="PK976_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67199.1"
                     /translation="GFNNDQKLAAIITPAANPNMELSTFLLMLLKKNTMDAPNAVISH
                     VNNVAIKASKAGLYCNKLSYFIPKNN"
BASE COUNT          600 a          371 c          352 g          592 t
ORIGIN      
        1 ggaaaaaatg tcaggttgaa attccagatt atgaaagtca aatagttttc atcgatggat
       61 ttccaataaa aatcctcaat aaagaaagaa gccctcaaaa tatcaactgg agaaacgaga
      121 aagtaagaat cgtagtggat tgcaccggaa agttcttaga ccctaccatt tcacctgccg
      181 aaggtaagga tgccttatgt ggtcatttga ctgctggtgc gcaaaaagta atttcaagcg
      241 ctccatttaa aattaaaaat gcttcgaccc atgatctcga tagcttccct actctcattt
      301 atggaataaa ccataccagt tatgaccctc taagacatca tataatttca gcagccagct
      361 gtactactac cggacttgct cacatgataa agcctcttct cgagaataaa gaaaccagta
      421 atgtgcttac agcttctatg tcgacagtcc atgccgctac caatacacaa agcgtgctcg
      481 attcagtccc tgcagccgga gcaacagatc tacgaaagaa caggtcggta ttcaataata
      541 ttattctttc gacaactgga gcagctaaaa cactcgaaat ggtcatacca caagtcacta
      601 acttcggctt tatggcagat tctataagaa tacccacaaa caccgtgtca ttgattgccc
      661 tcaatatcac tttcagctca agccttgatg agaaaggaat gccttctatt aatgccaata
      721 gcatcaaaga gatttataaa actgcagcca atggcaatca gaaagggtta ttggttttct
      781 ctaccaaaca aaatgtatcg gccgatttac ttggttttgc agcagccata gtgatcgaag
      841 gaaatgatat tcataccaga acgggctttc tgaaaattcc ccaagatgcc atatcctcct
      901 taggtattcc cgacatccac gaaataaata tccctgtcac tcatgcaaaa attatgggat
      961 ggtatgataa tgaatttggc agctatgtaa attgtctggg aaaattaacc gagcatatcg
     1021 catctcgaat ggagtgaagc attccttacg cattatgatc tagaatcttc gaattgaaaa
     1081 ttcggttaga agttaggcca aaagaacaat ccagattatt agttctttca ggcgatacgg
     1141 ataattttta ctgccgggct ttctcttcct tgaaaaaatt cctgatcact tcattctgac
     1201 gaattgtttc cacgtgggcg aagcgactga ctctaaactc agaactctta cttcgttttt
     1261 aatcaggttg tcgtgatttt gatgattgcg ttatagcatt acctattcta tataatcact
     1321 atcagtctta tcactccttg agacctactc caatcatctg caaccgttaa ttctttttta
     1381 aatttctttt cttgacgtta aattctaaaa ggttctcctt atgtgagaaa agaataacag
     1441 gcagaggtgc acagtgaact aaggatttga agtagttttt catagtgttt ctaagaaagt
     1501 acttttgtaa aatattgacg ggtaatgata agatcgggaa ttccgacatt tgagaaggtc
     1561 gaatcggtac tttttttata tatcataccg taaaccaatt cattgtattt tatcctcttt
     1621 attgattgac attagaatat cttccataat aaatagttta ggattattgt atggatgaag
     1681 attcttagat ttgagaaata ttttaattgt ttttgggtat gaagtacgaa agtttgttgc
     1741 aatacaatcc agctttactt gcctttatag caacattatt tacatggctg atgacggcat
     1801 tgggtgcgtc catggtattt ttcttcaaaa gcatcaataa gaaggtactt aattccatgt
     1861 tgggatttgc tgccggagtg atgatagcag caagtttctg gtcgttatta aagcc
//
LOCUS       DAORPD010000323         1911 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_11195_length_1911_cov_2.521552, whole genome shotgun sequence.
ACCESSION   DAORPD010000323 DAORPD010000000
VERSION     DAORPD010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11195_length_1911_cov_2.521552"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="PK976_04605"
     CDS             complement(<1..706)
                     /locus_tag="PK976_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005852577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HRC67200.1"
                     /translation="MAKKLKKHVYLFGNKTAEGSAEMKDLLGGKGANLAEMNLIGMPV
                     PPGFTITTEVCDAYYKLGREAVIEMIKDEVEAGIRFIENIMGTRFGDPNNPCLVSVRS
                     GAKASMPGMMDTILNLGLNDAVVEGMIKKTGNPRFVWDSYRRFVQMYGDVVLGMKPAS
                     KQDVDPFEEIIEELKEERGLHFDNELTTEDLKELVFRFKKAVRERTGKDFPENPWDQL
                     WGAIMAVFDSWMNDRAI"
     gene            1136..1717
                     /locus_tag="PK976_04610"
     CDS             1136..1717
                     /locus_tag="PK976_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008146779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HRC67201.1"
                     /translation="MEAKSIKGTRTEQNLLKAFAGESQAKNRYIFFAKKAREEGYEQI
                     AAIFELTAENEETHAKQFFRFLEGGMVEITASYPAGVIGTTMENLEQAATGEYEEWSQ
                     LYPEFARIAEEEGFKQIAVKFRTVAKVEIEHEKRYRKLLANIENGEVFKKKQKVRWIC
                     RKCGFVYVGESAPQRCPGCDHPQAFFELLTENY"
BASE COUNT          598 a          388 c          355 g          570 t
ORIGIN      
        1 agatagcacg atcgttcatc cacgaatcaa aaactgccat gatggcaccc cagagctgat
       61 cccatggatt ttcgggaaaa tctttccctg tacgttctct tacagccttt ttaaatcgaa
      121 acactaattc tttcaaatct tcggtagtca attcattgtc aaaatgtagg ccgcgttctt
      181 cctttaattc ttcgataatt tcctcgaaag gatccacatc ttgtttcgaa gcgggtttca
      241 tgcccaaaac gacgtctcca tacatctgta cgaaacgtct ataactatcc catacaaatc
      301 gtggattccc ggtttttttg atcatacctt ccactacggc atcattcaaa ccaaggttga
      361 gaatggtatc catcatgccg ggcatagagg ctttggcgcc ggaacgaaca gataccaaac
      421 acggattgtt aggatctcca aaccgagtgc ccataatatt ttctataaag cggattccgg
      481 cttctacctc atcctttatc atttcaataa cagcttcgcg gcccaattta taataggcat
      541 cacaaacctc ggtcgtgatg gtaaagccag gtggcacagg cattcctatc aaattcatct
      601 ctgcaaggtt ggctcctttg ccaccgagta aatctttcat ttcggcactt ccttcggctg
      661 tcttgttgcc gaataggtaa acatgttttt tgagtttctt tgccattttc agtttcaatt
      721 atttgggttg attattcaaa tgattttcaa tttcatgaaa tatttttcaa acaagcctgc
      781 aaaagtataa cttttcgtaa aatattctga ataacttaaa gatttcttat tacatctcac
      841 cactgacctc attttataaa tcttcgaaaa tatatgcttc accagaaaaa tcaaagtcat
      901 ggattcccat ttcactaaac tttctcttga tattctgaat gaaagggaaa tggtattatc
      961 attcatcacg aataaaaact aacaatgaaa gacttcaatt tgggtttctg attatcattt
     1021 ttccatatac atataaaaaa gtatatcttt gtagctatgt tgaaacattt cgatgactcg
     1081 aacttgaatc ttaaatgaaa aataaaacaa tcttcatctt aataatcatt ttgttatgga
     1141 agcaaaaagt ataaaaggga cacgaaccga acaaaatctg ttaaaggcct ttgcgggaga
     1201 atcgcaggca aagaatcgct atattttctt cgcaaaaaag gcaagggaag aagggtatga
     1261 acaaattgca gccattttcg agctaacagc cgagaatgag gaaacgcatg ccaagcagtt
     1321 tttcagattt cttgaaggag gaatggttga gattactgcc tcttatccag caggtgtaat
     1381 cggcacgact atggaaaacc tcgaacaagc agccaccggc gaatacgaag aatggtcgca
     1441 attataccct gaatttgcgc gcattgccga ggaagaaggg ttcaagcaga ttgccgtgaa
     1501 atttcgtacg gttgctaaag tagaaatcga acatgaaaaa cgctaccgta agttattagc
     1561 caatattgaa aacggagaag ttttcaagaa aaagcaaaag gtccgctgga tttgtcgtaa
     1621 gtgcggcttc gtttacgtag gagaaagtgc tcctcagcgt tgccccggat gcgatcatcc
     1681 tcaggcattt ttcgaattat tgaccgagaa ttattgaagg tttcctcgaa taatgacaaa
     1741 atccgatgaa cccttggtat taaggaatga aattaaaaaa tgtatcactt gttaaggttc
     1801 atcttattgc gttttcgaag ggtttttcaa attttcacga taggagagaa atcccactct
     1861 aaaggataaa ttattatatt tccgatatcg aagatgccat gctatgcatg c
//
LOCUS       DAORPD010000324         1911 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_11196_length_1911_cov_2.367457, whole genome shotgun sequence.
ACCESSION   DAORPD010000324 DAORPD010000000
VERSION     DAORPD010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11196_length_1911_cov_2.367457"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            767..1447
                     /locus_tag="PK976_04615"
     CDS             767..1447
                     /locus_tag="PK976_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008151428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HRC67202.1"
                     /translation="MGGIYIIFIAFAIISWLISNQLKVKFAKYSKMPIQYSGKEIAER
                     MLRQNGIDDVKVLSVEGQLTDHYNPANKTVNLSRDVYYGSNVAAAAVAAHETGHAVQH
                     ASAYAWLQMRSALVPVVSFASRWVQWVLLAGILVVNTFPQLLVAGIILFASTTFFSII
                     TLPVEVNASHRALVWLKGTGITNNQNQAAAFDALKWAAYTYFIAALASLATLVYYVLI
                     LMGARNRD"
BASE COUNT          547 a          377 c          362 g          625 t
ORIGIN      
        1 actcctcgaa gaaaaattcc tcaaagaagt gaagctctca ttcatataaa aaaaattgca
       61 tgatcccgat cttctatcac tcaatgaccg ctaaaccttt cattaaaata ttccctgatg
      121 catttcccac acattgactc cttttttcgg ggtgttcaac ccaacatacc gtatttttag
      181 cctcaagggc tatattgaaa atgcagtttt ccctgccatg aatttacccg acctcttctc
      241 caaaaattta gaatgtctca aatccgtcaa tgagtcttcc atttttcctt cgatattcga
      301 attgtttacg aatttctatt ggatcatctt cctgatgctg tgaaaggtta ctattggctg
      361 cataatttgg tcgttttcgt ctatcgcaga ttttgcccat ggcatgtatg ttattatata
      421 agagctgaaa tttcgatccc gtttaaaaag ttttttattg tataatctgt aataaattat
      481 ttctcggtag atatcaaaaa tcgactgctg ccattccatt atttatttgg aaggccaaat
      541 aattagatcg aatcgaaata tgttcttcga gctgagcaag tccaaagatg tgaatgaaaa
      601 gcgggctctt tcttatttct atcttccttc ttatcctgaa aattttcatg aatattatgg
      661 taataaaaat ggttttctcc gaagattgaa tagaaaggaa atgaggtatt ctctatcgga
      721 tggcataaaa cttgataatg ggggcgtaat aattcagatc gtagaaatgg gtggaatata
      781 tatcattttc attgcttttg caataattag ctggctgatc agcaatcagt tgaaggttaa
      841 atttgctaaa tattcaaaaa tgcctattca atatagcggg aaagaaattg ccgaacgtat
      901 gcttaggcag aacgggatag atgatgtgaa agtattatcg gttgaagggc aattgaccga
      961 tcactataat cctgcgaata aaacagtaaa tctgagccgg gatgtatatt atggcagtaa
     1021 tgtagctgcg gctgccgttg cggctcacga aactgggcat gccgtacagc acgcttcggc
     1081 ttatgcctgg cttcagatgc gttcagctct tgtgcctgta gttagttttg ccagccgatg
     1141 ggttcaatgg gtattgctgg caggaattct ggtggtaaat acttttcccc aacttctggt
     1201 ggcaggcatt attctttttg caagtactac ttttttcagc atcattactc ttcctgtcga
     1261 ggtaaacgcc agtcacaggg cattggtttg gctcaaggga acaggcataa caaataatca
     1321 aaatcaagct gcagcatttg atgccttgaa atgggctgcc tatacttatt ttatagccgc
     1381 tctggcatca cttgctactt tggtgtatta tgtcctgatt ctgatgggtg ctcgaaaccg
     1441 tgactaattg tcattccatc atttttagaa ccattctatg cttgaattta atatcaaact
     1501 tcctataaag gaaggatttc ataacccttg atcctatcat tgcatattga ttccttcagt
     1561 aatgagggga taaatcatgc tttagaatat cccaagaata acgagtctca tcgttctttt
     1621 gccctttacg ctttcgcgag cgatgttttt cttgcaaaga cgccaagaac gctaagaaaa
     1681 tcacttcaaa tccttagcga actttgcgta aagccatcta tccatcatca aaaccatgac
     1741 aactccgttt aaaactgaat caggagttct gaatttattt tgaaaatttt tgaggcttca
     1801 gggaattgaa atagcgttta cctcttatat aatagctcca cacaatactt ccgcggtaaa
     1861 ttcccgaaac ctgtcgggaa ggcaagtttt ctcttttgct catagtacgg c
//
LOCUS       DAORPD010000325         1910 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_11209_length_1910_cov_2.356334, whole genome shotgun sequence.
ACCESSION   DAORPD010000325 DAORPD010000000
VERSION     DAORPD010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11209_length_1910_cov_2.356334"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            168..659
                     /locus_tag="PK976_04620"
     CDS             168..659
                     /locus_tag="PK976_04620"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HRC67203.1"
                     /translation="MKNPSSKIHSSDEGLFEKAKRWCAFEERCSYDLRLKLEQWGVTE
                     DKISEIIQQLTREGFIDDGRFARMFVQGKFHNLKWGKIKIKAELQRHHISNELTEQAI
                     ALLDDDEYKKSLLKILNNKRKSLSKELPDVARQKLIRFALSKGYEAELIYSLLGKTNL
                     IEE"
     gene            680..1778
                     /gene="prfB"
                     /locus_tag="PK976_04625"
     CDS             join(680..739,741..1778)
                     /gene="prfB"
                     /locus_tag="PK976_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_157754648.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HRC67204.1"
                     /translation="MMNEDLKVLESRIEALRRFLDIDKKLKDVKEEEDITHANDFWND
                     PKSAEKILKSIQAKKVWIERFEKVKNLYEDLRVLVDFWDMGEATEEEVNRHYQETLQE
                     VEELEFMNMLSAEEDRLGAIMEINAGAGGTESQDWASMLMRMYIRYGERQNFKVTELD
                     LHEGDVAGIKNVVLEFDGEYAFGYLKAENGVHRLVRISPFDSNARRHTSFASVYIYPI
                     VDENIEIDINPADITWETYRSSGPGGQNVNKVETAVRLKHEPTSIVIECQVTRSQQQN
                     KEKALQMLKSKLYEIELRKKREKIDAIEAAKKKIEWGSQIRSYILHPYKMVKDHRTNY
                     ETGDTQAVLDGDIQDFIKAYLMQNGNIARKQ"
BASE COUNT          687 a          340 c          396 g          487 t
ORIGIN      
        1 gatggaatat tttattcaaa ggtaagcata aaatgtagat tagaaaagtt tttctgaatt
       61 ttttaagaga agtaaaaaat cccagcccaa tacatctaat aaatttaaac acaacattct
      121 ttctatatga acaagaagtt acataatttt gcagtttctt aatctatatg aaaaatcctt
      181 cttcaaaaat tcattccagc gatgagggtc tgttcgagaa ggcaaagcga tggtgcgcct
      241 ttgaggaaag atgcagctac gatctcaggc ttaaactcga gcaatgggga gtaactgaag
      301 acaaaatctc agaaattatt cagcagctta cccgtgaagg gtttatagat gatggacgtt
      361 ttgcccgcat gtttgtccaa ggaaaatttc acaatttaaa atggggcaag ataaaaatca
      421 aggccgagct tcaacggcac cacatatcga atgaattgac agagcaagcc attgccttgc
      481 tggatgacga tgaatataaa aaaagcttat tgaaaatctt gaacaacaaa cgaaaatcgc
      541 tatcaaaaga actgccagat gttgcccgtc agaaactcat acgttttgcc ctgtcaaaag
      601 gatacgaagc tgaactgatt tacagcttac tgggtaaaac aaacctcata gaagaataat
      661 tttttaaatg actcaaaata tgatgaatga agaccttaaa gttctcgaat caaggataga
      721 agccttgagg aggtttcttt gacatagata aaaaactcaa agatgtaaag gaagaggaag
      781 acataaccca tgcaaacgac ttttggaatg atcccaaatc tgcagaaaaa attcttaagt
      841 ccattcaggc aaaaaaagta tggatagagc gttttgagaa agtaaagaat ttatatgaag
      901 atttacgggt tttggttgat ttttgggata tgggcgaagc caccgaggaa gaggtgaata
      961 ggcattatca agaaaccctt caggaagtag aagaacttga atttatgaat atgctttcgg
     1021 ccgaggaaga ccgtctgggt gccatcatgg aaatcaatgc tggggcagga ggaacggaga
     1081 gtcaggactg ggcttccatg cttatgcgca tgtacattcg atatggcgag aggcagaact
     1141 tcaaggtgac cgaactcgat ttacatgaag gcgatgtagc aggaataaaa aatgtagttc
     1201 ttgaatttga tggtgaatat gcttttggat atttgaaggc cgaaaatggg gtgcatcgtc
     1261 ttgtgcgcat ctcacctttc gattcgaacg cacgacgtca tacttctttt gcatcagttt
     1321 atatatatcc tatcgttgat gaaaatatag aaatcgatat caatccggcc gatattacat
     1381 gggaaaccta ccgcagtagc ggcccgggcg gacaaaatgt taacaaggta gaaaccgctg
     1441 tcaggcttaa acatgaacct acgagcattg tcatcgaatg tcaggttaca cgctcacagc
     1501 agcaaaacaa ggaaaaagcc ctgcaaatgc tgaaatcgaa actctatgaa atagaactgc
     1561 gcaagaaaag agaaaaaata gatgccattg aagctgcaaa gaaaaaaatt gaatggggat
     1621 cacaaatacg tagctatatc ctgcatcctt ataaaatggt gaaagatcac cgaaccaatt
     1681 atgaaaccgg agacacacaa gcagttctcg atggtgatat tcaagacttc atcaaggcct
     1741 acctgatgca aaatggcaac attgctcgaa aacaataata tacaaaattc gaagtatgca
     1801 tcagctttac accccttttt taaaatgtcc gaatcatcct ttaaccataa aatcggaata
     1861 ataaatgcca attgtcatta aaaaaaccta tttttaccgg aacttttatc
//
LOCUS       DAORPD010000326         1909 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_11218_length_1909_cov_2.490831, whole genome shotgun sequence.
ACCESSION   DAORPD010000326 DAORPD010000000
VERSION     DAORPD010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11218_length_1909_cov_2.490831"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1887
                     /locus_tag="PK976_04630"
     CDS             <1..1887
                     /locus_tag="PK976_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRC67205.1"
                     /translation="VEGIFYDAWAYFLGIERKINNNHSLALTVYGSPTRRGQQGPATQ
                     EAYDLSETNFYNPYWGYQQGDKRNSRVKDFHEPMILLNHYGKLSENLQWNNGLAVSFG
                     YNGGTALNWYNAPDPRPDYYRYLPSYATDENIRKMVADRWKSSENVRQINWDKLYQIN
                     YLSNLEGKGARYIVENRRNDQLQFLINSTLNYQINQNIALSGGATYRNYRGSHFKLIE
                     DLLGSNYWLDIDQFAERDFTGDTIKAQNDLNNPNRKVSEDEKFGYNYCVYQQLLQAWV
                     QTEFSYDAGNFYAGIAYTGQQFYREGLMRNGRHPDNSFGDSQKYNFNDATLKTGGTYQ
                     IFPRNFIQVNGMYMTRAPFIQNVFISPRTRADVLPQMVSEKIVSGEASYLMRYPFLNL
                     RFTVFHTIFDDQSEVNSFYHDDLKTYVNYVLTGISKTHQGLEFGTEVKITSALSLQAV
                     AAWGNYRYTNRPLATICYDNGSKPDTTERGYIKNFYVPGTPQTAASLGLKYAGRRYLY
                     INLNANYFDRNYVDFNPERRTSAAIANLGPGDPLITTITQQEKLPDAFTLDASIGKSW
                     RWGEYFLNLNFSVSNILDNKKVITTGFEQMRFDFTNKDLAKFPPKYYYGYGRNFFLNL
                     GFRF"
BASE COUNT          590 a          442 c          367 g          510 t
ORIGIN      
        1 gtagaaggaa tattttacga cgcatgggct tattttttag gcatagaacg caaaatcaac
       61 aataaccata gtttagcctt aacagtttat ggctcaccta cccgccgtgg acagcaaggt
      121 ccagctactc aggaggctta tgacctgtca gaaactaact tttacaaccc ctattggggc
      181 taccaacaag gagataaacg aaattctcga gtaaaagatt tccatgaacc catgatcctc
      241 ctcaatcatt atggaaaact cagcgaaaac ctacaatgga acaacggtct ggccgtaagc
      301 tttggatata atggaggaac agccttgaat tggtacaacg ctcccgatcc acgtcccgat
      361 tattacaggt acttacccag ttatgccacc gacgaaaata tccgcaagat ggttgccgac
      421 cgttggaaaa gttcggaaaa tgtccgccaa atcaattggg ataaacttta tcaaatcaac
      481 tatttgagca atttagaagg gaaaggagct cgttatatcg tggagaaccg tcgcaacgat
      541 cagcttcaat tcttgatcaa ttcaactctc aattatcaaa tcaaccaaaa catcgcactt
      601 tctggaggag ctacttacag aaattaccgt gggtctcatt ttaaactcat agaagatttg
      661 ctgggatcga attactggct cgatattgat cagtttgccg aaagggactt cacgggtgat
      721 accatcaagg ctcaaaatga tctgaataat cccaatcgga aagtttccga agatgaaaaa
      781 tttggatata actactgtgt ctatcaacaa ttgttacaag cctgggtgca gaccgaattt
      841 tcgtatgatg caggcaattt ttatgcaggt attgcataca cagggcaaca attctatcgc
      901 gagggcctga tgcgtaatgg ccgccacccc gataattcct ttggcgattc tcaaaaatac
      961 aatttcaatg acgctacatt aaaaacgggt ggcacatatc aaatctttcc ccgtaatttc
     1021 attcaggtaa acggaatgta tatgacccgg gcacccttta tccagaatgt tttcatttca
     1081 ccacgtactc gagccgatgt actgccccaa atggttagcg aaaaaatcgt gagcggcgaa
     1141 gccagttact tgatgcgtta cccctttttg aacctacgtt ttacggtttt tcacaccatt
     1201 ttcgacgacc agagcgaagt aaacagtttt taccacgatg atttaaaaac ttatgtcaat
     1261 tatgttctca caggaataag caagactcac caaggattag agttcggaac agaagtgaag
     1321 attacttcgg ctttgtcgct gcaagcggtt gcagcttggg gaaactatag atataccaat
     1381 cgtcctttag ccactatttg ttatgataat ggctcgaaac ccgataccac cgaaagggga
     1441 tatatcaaga atttctatgt cccgggaaca ccacaaacgg ctgcctcttt aggtttaaaa
     1501 tatgctggtc gacgttatct ttacatcaac ctcaacgcca attatttcga ccgtaattat
     1561 gtagatttca atcccgaacg ccgtacttcg gcggccattg ccaaccttgg ccccggcgat
     1621 cccctcatta ccactatcac tcaacaggaa aaactgcccg atgccttcac cctcgatgct
     1681 tcaatcggaa aatcatggag atggggagaa tattttctga acctcaactt ctccgttagc
     1741 aatatactcg acaacaaaaa ggtgattact accggattcg agcaaatgcg cttcgacttc
     1801 actaacaaag acttagccaa attcccaccc aaatattatt atggatatgg aagaaacttc
     1861 ttcctaaatc tcggttttcg tttttaaatt cctcatccaa aagaaaaat
//
LOCUS       DAORPD010000327         1909 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_11219_length_1909_cov_2.279396, whole genome shotgun sequence.
ACCESSION   DAORPD010000327 DAORPD010000000
VERSION     DAORPD010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11219_length_1909_cov_2.279396"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(389..703)
                     /locus_tag="PK976_04635"
     CDS             complement(389..703)
                     /locus_tag="PK976_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67206.1"
                     /translation="MRFLSVGAYQYWSREDTIYNNKLYHILYLEQHCLSLVTFKYCEA
                     IKEDNLKRIYIIDCNCAACGENEVILYDFSKTIDNTVFVGVKGLWSGKLLNHNRNIFD
                     SR"
     gene            1162..>1909
                     /locus_tag="PK976_04640"
     CDS             1162..>1909
                     /locus_tag="PK976_04640"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRC67207.1"
                     /translation="MIEDYFSPFRKNIVGNERMIETPFGCRKLIYADWIASGRLYKPI
                     EEKMLNVFGPWVANTHTETSETGTLMTKSYQYAHQLIKQHVHASPHDVIITYGFGMTG
                     VVNKLQRILGLKFCGRLFSSTCLAERDKPIVFITHMEHHSNHTSWFETAADVEVIGHT
                     PNLEVDLNDLEDKLKKYKDRKFKIASVTACSNVTGIQPPYYEIAKLIHSYGGLCFVDF
                     AASAPYVDINMHPDNPLEKLDAIFFSPHKFL"
BASE COUNT          598 a          355 c          341 g          615 t
ORIGIN      
        1 gggtaatgaa aaaaactggg aatcttgctt ggatatattg aaaacatcgc tgatcctgtg
       61 tcttagcccc ataaattgta aaattcaagg aacttataac ctctatagaa atcattgagt
      121 tatgcttgtt cctattaccg gatggtgaaa tatatttaac ttggattttt ccgaatgaga
      181 aggaactcct tcaaataaca gaaaacaagt ttcataatca ggattcagat attttacggt
      241 atcattttgc ctcatacata ttaatttctt cacaataaaa cctgtaggga aagggtaaat
      301 aggcgaaagc aacccgaagg tactaccaat actattcatc catttttcta catactcgta
      361 aaagtgaaat ctctttctgc atccatcttc aacgagaatc gaatatattt cggttatgat
      421 tcaatagctt gcccgaccaa aggcctttca caccaacaaa aacagtgttg tcaatggttt
      481 tcgaaaagtc atataatatt acctcatttt ctccacacgc agcacaattg caatcgatta
      541 tataaattcg tttcaaattg tcttctttga tagcttcgca atacttaaaa gtaactaagg
      601 ataaacagtg ttgctcaaga tataatatgt ggtaaagctt gttgttataa atagtgtcct
      661 cacgagacca atattgatag gctcctaccg acaaaaaccg catgaattgt tctattttac
      721 tccatatctt attgtgactc tttctccaat ttgtttcgga gtttcatcaa ggcgagtcga
      781 gagcctttct attaaaaggg atgaatatgc gatgatgggg taatttctct tttttcttca
      841 aaaaactcat tgaatttcaa aaattcaagt gataggataa tttttctatt tgtcaaagtt
      901 tagtaaaaaa gttgaaaatg tttttaggaa aattaaaagc ttgggttgaa aatataaaat
      961 ataaacagat ggtaattggc gatgggggaa taaagaatga aaaggagtta tttttatatt
     1021 tcttcttctt tttcctatta gcatattttc ctgtttcctt ggcttaaaat ccccaatgtt
     1081 tgtgcactcc ttgcaacatt atatttcata ctccttcaca aaaacctgta aatttgcccc
     1141 attttccgaa tcattcgttt tatgattgaa gattactttt cgccatttcg taagaatata
     1201 gttgggaatg aaaggatgat cgagactcct tttgggtgca ggaaactcat ctatgccgat
     1261 tggattgcaa gcggcaggct ctacaaacct atcgaagaaa aaatgttgaa tgttttcggc
     1321 ccctgggtag ccaatactca taccgaaacc agcgaaactg ggacactgat gaccaaatcg
     1381 taccaatatg ctcatcagtt gatcaaacaa catgtacatg ccagtcctca tgatgtcatc
     1441 atcacttatg gttttggaat gacgggtgtt gtaaataaat tgcagcggat tcttggattg
     1501 aaattttgtg gacgtttatt cagttccacc tgtcttgctg aaagggacaa acccattgtg
     1561 tttattacgc acatggaaca tcacagcaat catacttcat ggttcgaaac cgcggccgat
     1621 gtcgaagtga ttggtcatac cccaaacttg gaagtcgatt taaatgatct ggaagacaag
     1681 ttaaaaaagt ataaagacag aaaatttaaa attgcctcgg tcacggcttg ttcgaatgtt
     1741 acgggaatac agccacccta ttacgaaatt gccaaactca ttcatagtta tggaggtttg
     1801 tgttttgtag attttgccgc ttcagctcct tatgtagata ttaacatgca tcccgacaac
     1861 ccgctcgaga agctcgatgc tattttcttt tcgccccata agtttcttg
//
LOCUS       DAORPD010000328         1909 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_11221_length_1909_cov_1.853830, whole genome shotgun sequence.
ACCESSION   DAORPD010000328 DAORPD010000000
VERSION     DAORPD010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11221_length_1909_cov_1.853830"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /gene="serC"
                     /locus_tag="PK976_04645"
     CDS             <1..889
                     /gene="serC"
                     /locus_tag="PK976_04645"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HRC67208.1"
                     /translation="IPEGYHVLFLGGGASLQFCMVPFNLFNKKAAYLETGVWAKKAIK
                     EAKLFGEVVIPASSSDKNFNYVPKGFTIPTDVDYFHITTNNTIYGTELREDLDSPVPL
                     VADMSSDIMSRPLDVSKYAIIYGGAQKNVGPAGVAFVIIREDVLGKVERNIPTMLDYR
                     THIKEGSLFNTPPVLPIYTLMQTLRWLKGLGGVSEMEKINKQKANLLYDYIDQSKMFV
                     GTAEKDSRSLMNVCFVMKPQYTDKEEAFMDFAKNKGMVGIKGHRSVGGFRASLYNALP
                     IESVKALIACMEEFEKANA"
     gene            1030..>1909
                     /gene="amrB"
                     /locus_tag="PK976_04650"
     CDS             1030..>1909
                     /gene="amrB"
                     /locus_tag="PK976_04650"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HRC67209.1"
                     /translation="MKLTNFLLIFTVFWMTTVGCHSQNKGGNMQSIDRKPAVAGQFYP
                     ADSASLQKSLENLFVQAKPGPTLNLRAIIVPHAGYIFSGKVAASGFNQIPADMKYENI
                     FIIGPSHRAAWDGASVYDRGDYITPLGKATVNTQLASELIKKYSFFSYRQDADAYEHD
                     IEVQIPFLQKHLKNPFKIVPIIIGTDSPETLKKIASALKPWFNPNNLFVISTDFSHYP
                     SFEDAVNVDRVTAQAICTNSPQNLLDTLISNRKKHISGLSTSLCGSSAVFTLLYITFE
                     LPGIEIKDIDYQNSGHS"
BASE COUNT          575 a          444 c          374 g          516 t
ORIGIN      
        1 cattcccgaa ggatatcatg tactgttcct gggagggggt gcgtccttgc agttttgtat
       61 ggttccattc aacctcttca acaagaaagc agcctatctc gaaacaggcg tatgggccaa
      121 aaaagccatc aaagaagcta aactctttgg cgaagtagtc atacctgctt cgtcaagcga
      181 caaaaatttc aactatgtcc ccaagggttt taccattccc accgatgtag attacttcca
      241 cattactacc aacaacacga tttatggaac cgaacttcgt gaagacctcg acagccccgt
      301 tcctctggtt gccgacatgt cgtccgacat catgagcagg cctttggacg tttcaaaata
      361 tgccatcata tatggaggtg cacagaaaaa cgtcggtcct gcaggagtcg ctttcgttat
      421 aattcgtgaa gatgttctgg gcaaagtgga acgcaatatt cccaccatgc tcgattatcg
      481 tacacacatc aaggaaggat cgttattcaa tactcctcca gttctaccta tttacaccct
      541 tatgcagacc ttgcgctggc tcaaagggct gggcggagtg agcgaaatgg aaaaaatcaa
      601 caaacaaaag gctaatctgc tttacgacta catcgaccag agcaagatgt tcgtgggaac
      661 agccgaaaaa gacagccgtt cgctcatgaa tgtatgcttt gtaatgaaac cccaatatac
      721 cgacaaggag gaagccttca tggactttgc aaaaaacaag ggaatggttg gaattaaagg
      781 tcaccgttca gtaggcggtt ttagagcctc cttatacaat gctttgccca tcgaaagtgt
      841 aaaagctctg attgcttgca tggaagaatt cgaaaaggca aatgcctgaa tgccctctcg
      901 aaaagaataa ttagaaaacc gtactctttc agtacggttt ttctgttttg tataaaaatt
      961 ttcattgctc ggaaaattta cagtttttta tactaaatta gcattcctat tttaaataga
     1021 ctccaaaaca tgaaacttac taattttcta ttgatattta cagtgttttg gatgactacc
     1081 gtgggttgtc attcacaaaa taagggagga aatatgcaaa gcatcgatcg taaacctgct
     1141 gtggcgggtc agttttatcc ggccgatagt gcttcactgc aaaaaagtct cgaaaacttg
     1201 tttgtacagg caaaaccggg cccaactctc aatctcagag ccatcatagt gcctcatgca
     1261 ggctatattt tctcgggaaa agtagctgca tcggggttca atcagatccc agccgacatg
     1321 aaatacgaaa atatttttat cattgggcca agccaccgcg cagcctggga tggagcaagc
     1381 gtttacgaca ggggcgatta tattaccccc ttaggcaaag ctacagtaaa cactcaattg
     1441 gccagcgaac tcataaaaaa atattctttc ttttcttatc gccaagatgc cgatgcctat
     1501 gaacacgaca tcgaggttca gattcctttc ctgcaaaaac atcttaagaa tccattcaaa
     1561 atcgttccaa tcatcattgg tacggattca cccgaaaccc tcaagaaaat tgcgtcagca
     1621 ttaaaaccat ggttcaaccc aaacaatctt tttgtaattt cgaccgattt ttcacattat
     1681 ccttcattcg aagatgcggt gaatgtggac agggtcactg cacaagctat ttgtaccaat
     1741 tctccccaaa accttctcga taccttgatt tcgaatcgta aaaaacatat ctccgggctt
     1801 tcgacaagcc tctgtggctc ttctgcagtt ttcacactgc tttacattac tttcgaatta
     1861 cccggaatcg aaatcaaaga catcgactat caaaattcag gtcacagtc
//
LOCUS       DAORPD010000329         1906 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_11253_length_1906_cov_2.621826, whole genome shotgun sequence.
ACCESSION   DAORPD010000329 DAORPD010000000
VERSION     DAORPD010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11253_length_1906_cov_2.621826"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="PK976_04655"
     CDS             <1..625
                     /locus_tag="PK976_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67210.1"
                     /translation="VELENKLGELKYCLRLGNRPISTSKKPIVKSECARIGSVINNAD
                     NVIIKYDYENCYEDPENNILVKYRFGTTDVQKQFYFDVAANKTSIFVSDPLHFKTILK
                     ENEMVTKSILDLTIHSKYDSPFTIEKVVLEWKGLAPIIIDNIQQNFKGKGSHNLRLNI
                     NQPININETSALGKTLPVVSGYIYYRSSATGELMSYRIYNQSYTTDW"
     gene            822..>1906
                     /locus_tag="PK976_04660"
     CDS             822..>1906
                     /locus_tag="PK976_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67211.1"
                     /translation="MHEKKNRILAAILLGIFAIIILLIAFLSPGIGGEGDSFTHYFIA
                     RHAASDPKWLLDLWGRPLFTLLATPFAQFGYSGMKIFSILMALITAWLGYLMADKMKF
                     QHPWLAIPFIILTPLYFNQIFSPLTELAMAMFLMAGIYTYISKKYILSAILFSLLPFI
                     RLEAFVILPVVAIAFLVDRKYPHILLLLTGYLIFSCIGYFLFKDFLWIYHKNPYQSGA
                     TEIYGKGSLLHFIRHTDKIQGYLMASLFLLGLIGWGLQIIKKKGELYLGENHLGVYII
                     TAGSFLIYYAAHSYSWWSGKGNSLGLIRVIAAVLPAASIISHAGYTLLINYLKKFKIP
                     PAITAVLVLILLMRTTLVYPYPIRPYA"
BASE COUNT          566 a          394 c          333 g          613 t
ORIGIN      
        1 ggtagaactg gaaaacaaat tgggcgaatt gaagtattgc ctccgtttag gcaaccgccc
       61 catctcaact tctaaaaaac ccatcgtgaa atcggaatgt gcaagaattg gttcagttat
      121 caataatgcc gacaatgtca tcattaaata cgattacgag aattgttacg aagaccccga
      181 aaacaatatc ctcgtaaaat accgcttcgg aacaaccgat gtgcaaaaac agttctattt
      241 cgatgtagcc gctaacaaaa cctctatttt tgtgagcgac cccttacatt ttaaaaccat
      301 cctcaaagaa aacgaaatgg ttaccaaatc cattctcgat cttacaatcc attcaaaata
      361 cgattctccc tttaccatcg agaaagttgt tttagaatgg aaaggcttgg ctcctattat
      421 catcgataat atccaacaaa atttcaaggg gaaaggtagc cataatctaa gactgaacat
      481 caatcagcca attaatataa atgaaacctc ggctttaggg aagacccttc ccgtggtttc
      541 gggctatatt tactacagat cttccgccac aggcgaattg atgagctacc gcatctacaa
      601 ccaaagctac acaaccgact ggtaatagaa aatcaaatta ataaaattcc ttcttatcct
      661 ctgtttattt atagatttta tgtagaaatt cgacgaaaat tccattctcg accactcctt
      721 ctgctcttat aaaatcgata ccgtctgata tttcgaaagg aaatgaattt ttagggttta
      781 atgaaacatt cataaatttg taaatctgta acctatctaa aatgcatgag aagaaaaatc
      841 gaatacttgc agccattttg ttgggaattt ttgcaatcat tatcctactc atagcatttt
      901 tatcgccagg gattggggga gagggagatt catttactca ttattttatt gcaaggcatg
      961 ctgccagcga tcccaagtgg cttcttgatc tttggggaag accgcttttt actcttctgg
     1021 ccaccccctt tgcacaattt ggatattcgg gcatgaaaat cttttcaatt ttaatggcct
     1081 taattacagc ctggctggga tacctgatgg ccgataaaat gaaatttcaa cacccatggt
     1141 tagctatccc gttcattatt cttacaccat tgtacttcaa tcaaatattc tctcctctca
     1201 ccgaactcgc tatggccatg tttttaatgg cgggtattta tacatacata agtaaaaaat
     1261 atattctttc ggccattttg ttcagtttgc ttccttttat acggctcgaa gcctttgtta
     1321 ttctacccgt agttgctata gcatttttag tcgatagaaa atatcctcat atccttcttc
     1381 ttttgaccgg ttatcttatt tttagctgca tcggatattt tcttttcaag gattttctgt
     1441 ggatctatca taaaaatcct taccagtcgg gtgccacaga aatttatggc aaaggcagtt
     1501 tacttcattt cattcgccat actgacaaaa tacaggggta cctgatggcg tcattattct
     1561 tgttaggatt gataggatgg ggacttcaaa ttataaagaa gaagggagaa ctttatttag
     1621 gtgagaatca cttaggggtt tatattataa cagctggaag cttcttgata tattacgcag
     1681 ctcatagtta ttcatggtgg tcgggcaagg gtaatagtct ggggttaatc agggttattg
     1741 cggccgtctt gccagctgcc tccattatta gccatgcagg atatactcta ctaatcaatt
     1801 atctcaaaaa atttaaaatt cctcctgcta ttacagcggt tttagtatta attctcttaa
     1861 tgcgcaccac attggtttat ccttatccta ttcgtcccta tgccga
//
LOCUS       DAORPD010000330         1904 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_11272_length_1904_cov_2.389941, whole genome shotgun sequence.
ACCESSION   DAORPD010000330 DAORPD010000000
VERSION     DAORPD010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11272_length_1904_cov_2.389941"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1719)
                     /locus_tag="PK976_04665"
     CDS             complement(<1..1719)
                     /locus_tag="PK976_04665"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HRC67212.1"
                     /translation="MNFKTIIFLSALLLVNFYGMTQVRVKVWSDEFNYSGLPDTNLWG
                     YDVGAGGWGNNESQYYTDHRWLNARAESGKLIIEARKENFEDCEYTSARLVTRNKGDW
                     KYGRIEVSARLPHGRGTWPAIWMLPTDWVYGSWVKSGEIDIMEYVGFDPGIVHGTIHT
                     QAYNHIIGTQKTDTFYVPDAETNFHLYAIEWTEEKIDFFVDDEKYFSFYNEHTGYTTW
                     PFDQRFHLLLNIAVGGSWGGQQGIDPDIWPQKMWVDYVRVFQNIPFEEIHIEGPCCLQ
                     AGQANAVFEIQDIEGAIFHWTVPEGATIESGQGTHKIKVTWGNDEENVKCEVIHPHAG
                     GIYSKEVFIALAPVYSRYDLANFAIQDTEGWTITANPPNQVNIFKQDTLMQLNYSIFP
                     NAGKPYLEYTFPTIVDMNQHPHLNVFLKTFNKSGTAATKIELFDSRKRFTDVDPGFSI
                     LPLQLSGEIQAFHFDFTNHWNTNLPFPNSKVDSNCISGLRIYLDTPGSECPCFDSLWI
                     SRIEISKENYLGISNRQSEPFIRFYPNPFRQTLYIKSSKNFPDHFYIFDLTGREVFHT
                     IINQPVN"
BASE COUNT          585 a          384 c          367 g          568 t
ORIGIN      
        1 gttgacgggt tgattgataa ttgtatgaaa aacttctcta cctgtgaggt caaagatata
       61 aaaatgatcg ggaaagtttt tactcgattt tatgtaaaga gtttgtcgaa aaggattggg
      121 ataaaaacga atgaagggct cagattgtcg gttgctgatt cccaaataat tttccttgct
      181 gatttcgatt ctcgatatcc agagtgaatc gaagcaagga cattcggaac caggtgtatc
      241 caagtatatt ctcaagcctg aaatacagtt ggaatctacc ttggaatttg gaaagggtag
      301 attggtgttc caatggttag taaaatcgaa atggaaggct tgaatttcgc ctgaaagttg
      361 caaaggcaga atcgaaaatc cgggatcaac atcggtgaat cgctttcgag aatcgaaaag
      421 ttcgatcttt gtcgctgcag taccactttt attaaaagtc ttcagaaaca cgttcagatg
      481 aggatgctgg ttcatatcta caattgtagg aaaagtatat tccagataag gtttacccgc
      541 atttggaaat attgaataat tgagttgcat caaggtatct tgcttgaaga tattgacttg
      601 attgggtgga ttagcggtaa ttgtccatcc ttcagtatcc tgaatggcaa aattggcgag
      661 atcatacctc gaataaacag gagcaagagc aatgaatacc tccttcgaat aaattccgcc
      721 agcatgagga tgaatgactt cacattttac attttcttca tcatttcccc atgttacctt
      781 gattttatga gtcccctgtc ccgattctat agtagctcct tcaggaacag tccaatgaaa
      841 tatagctcct tctatatctt gaatttcaaa aacagcatta gcttgtccgg cctgaaggca
      901 gcatggacct tcaatatgaa tttcctcaaa aggaatattc tgaaaaactc ttacataatc
      961 tacccacatt ttttgtggcc agatatctgg gtctattcct tgttgtcctc cccagcttcc
     1021 gccaacggca atattcaata ataaatgaaa gcgctgatcg aagggccaag tagtgtaacc
     1081 cgtatgttcg ttatagaaac tgaaatattt ttcatcatct acgaagaaat ctattttttc
     1141 ttctgtccat tcaatggcat agagatggaa gttggtttcg gcatcaggaa cataaaatgt
     1201 atccgtcttt tgtgtgccaa taatatgatt ataggcttgt gtatgaatag tgccatgaac
     1261 tataccagga tcgaaaccaa cgtattccat gatgtcgatt tcaccacttt taacccaact
     1321 gccataaacc caatcggtag gcaacatcca gatggctggc catgtgcctc gaccatgcgg
     1381 taatcgagca gatacctcga tgcgaccata tttccaatct cccttgttgc gcgtaacaag
     1441 ccttgccgag gtatattcac agtcttcaaa attttctttt cttgcttcaa taatcagctt
     1501 accactttcg gcccttgcat ttaaccaacg atgatccgtg taatattgag attcgttgtt
     1561 cccccatcca ccagctccaa catcatatcc ccaaagatta gtatcgggca ggccgctata
     1621 attaaattca tccgaccaga ccttcaccct cacttgtgtc atgccgtaaa agttaaccag
     1681 caataaagcc gaaagaaaga ttatcgtttt gaaattcact gaattatata aaatatcaaa
     1741 tggccataaa atttatataa agataatgga aatacaggat gtggaaataa agaacggcga
     1801 gcttttttta tgtatttggg ggaactttct cttcgccaag ccgtgaaatt ccagaaaaaa
     1861 aatgtcggaa ccacaaaaac accaatatcg ttgggcttat ttac
//
LOCUS       DAORPD010000331         1902 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_11291_length_1902_cov_1.639415, whole genome shotgun sequence.
ACCESSION   DAORPD010000331 DAORPD010000000
VERSION     DAORPD010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11291_length_1902_cov_1.639415"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1902)
                     /locus_tag="PK976_04670"
     CDS             complement(<1..>1902)
                     /locus_tag="PK976_04670"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HRC67213.1"
                     /translation="FNANAISQADYKLEHLVYQMNYEAARLAIEVARQYSTPTKPRFV
                     AGSIGPTNKTLLLSPDINNPANRAISFEQMEEAYVTQVEALLDGGVDILLIETIFDTL
                     NAKAALLACKRVLQAKGKDIVMTARGLKRRFPIMVSGTISDASGRTLSGQTPEAFLIS
                     LSHAELLSIGFNCSMGALQLRPYIEEIASKAPFYISVYPNAGLPNQFGEYEQSPDEMA
                     HDLAPLLHASKVNIIGGCCGTTPEHIRKLVEMVNLSQPHLPSPRHEALMLSGLDPLVQ
                     FPGSNFINIGERTNVSGSKKFARLIREERFEEALQVARQQVENGAQILDINMDDALLD
                     SERCMKIFLHHLMSDPEIARIPLMIDSSKWSVIETGLQCSQGKCIVNSISLKEGEKVF
                     IQHARTILDYGAAVVVMAFDENGQADTFERRISICQRAFRLLTHKVGFHTSDIILDAN
                     VLAIATGMSEHNQYAVEFIRAVKWIKENLHGVRTSGGISNLSFSFRGNDKIRQAIHSV
                     FLYHAIQAGLDMGIVNAGALPVYEEIPEPLLTLVEDVILNRRKDATSRLVMYANQYGK
                     KAETDSLITHNWRSDSLEKRLEHALIKGISDFLEEDMKEALETYPSALQVIEGPLMEG
                     MNHIGELFG"
BASE COUNT          499 a          421 c          434 g          548 t
ORIGIN      
        1 aaccaaacag ctctcctata tggttcatcc cttccatcaa gggtccttcg atgacttgaa
       61 gcgcagaggg ataagtttcg agagcctcct tcatatcttc ttccagaaaa tcacttattc
      121 cttttatcaa ggcatgctcg agacgtttct ccaatgaatc gcttctccag ttatgagtta
      181 ttaagctatc tgtttcggct tttttgccgt attggttcgc atacatcacc agacggcttg
      241 tggcatcttt acggcggttt aatattacat cttccaccaa agtaagtagg ggttcgggga
      301 tttcttcata cacgggcaag gcccctgcgt tcacgatgcc catatcgagt ccggcttgta
      361 tggcatgata aaggaaaaca gaatgtatgg cctggcgtat cttgtcgttg ccacgaaaac
      421 taaaagaaag attactaatt cctccactgg ttcttacacc atgcaaattt tcctttatcc
      481 atttcacggc tctgataaat tctacagcat attgattgtg ttcgctcatg cccgtagcga
      541 tagccaatac attggcatcg agtataatgt cgcttgtatg gaaacccact ttatgggtga
      601 gtaaacgaaa ggctctctgg caaatggaga ttcttcgttc gaaagtatcg gcttgtccgt
      661 tttcgtcgaa agccatcact acaaccgctg caccataatc cagaatagtc cgtgcatgtt
      721 gtataaagac tttttctcct tctttgagac tgatggaatt gacgatacat tttccctgag
      781 aacattgcag cccggtttct attacactcc atttggaaga atctatcatc aaaggaatcc
      841 ttgcaatttc gggatcagac atgagatggt gaagaaatat cttcatgcaa cgttcggaat
      901 caaggagagc gtcgtccata ttgatatcga gaatctgagc tccgttttcg acctgttgac
      961 gagccacctg aagggcttct tcgaatcttt cttctcgaat aaggcgggca aatttcttag
     1021 aaccgctcac attggttcgt tctccaatat taatgaagtt gcttcccgga aattgaacca
     1081 gagggtcaag tccactgagc ataagagctt catgacgagg agatggaaga tgtggctggg
     1141 aaagatttac catttccacg agttttcgaa tgtgctcggg ggtcgtacca caacatccac
     1201 caataatatt cactttggaa gcatgaagaa gaggtgcgag atcatgggcc atttcatcgg
     1261 gcgactgctc atattcgccg aattggttgg gcaggccggc attgggataa acacttatgt
     1321 aaaaaggtgc cttggaggca atttcttcta tatatggcct gagttgcagg gctcccatcg
     1381 aacaattgaa accaatgctc agcagctcgg catgtgaaag agaaatgagg aatgcttcgg
     1441 gtgtttggcc agagagggta cggccgctag catcgctaat tgttcctgaa accatgatgg
     1501 gaaatctgcg ttttaacccc ctggcagtca tcacaatgtc tttccctttt gcctgaagaa
     1561 cccttttgca agccaacaaa gccgctttgg cattcaaggt gtcgaatatc gtttcgatta
     1621 acagaatatc tactccacca tcgagcaaag cctcaacctg agtaacataa gcctcttcca
     1681 tttgctcgaa acttatggct cggttagctg gattatttat gtcgggagaa agtaagagag
     1741 ttttattggt ggggccaata gatccggcaa cgaatcgtgg tttcgttggt gtggaatatt
     1801 gtctggctac ctcaatagct aggcgggcag cctcataatt catctggtaa acaagatgtt
     1861 cgagtttata atcggcttgt gagatggcat ttgcattgaa gg
//
LOCUS       DAORPD010000332         1900 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_11308_length_1900_cov_2.539295, whole genome shotgun sequence.
ACCESSION   DAORPD010000332 DAORPD010000000
VERSION     DAORPD010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11308_length_1900_cov_2.539295"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="PK976_04675"
     CDS             complement(<1..556)
                     /locus_tag="PK976_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HRC67214.1"
                     /translation="MKKLTIILPYLLLMFMENYAQEEIKFQLPPPEILQLVDVKPQPA
                     VYIDYHNRYMLLFERKAFKNLEELAQPELRLAGLRIDPQTHAPSRIRYFYGVQLIDLS
                     TGKEVTLKNIPDSLNIANVSFSPDSRKLAFTNTVGRNISLWIIELETGMARMIYHQRL
                     NACMGAPYQWTPDSESLLVSVVPEN"
     gene            complement(633..1805)
                     /locus_tag="PK976_04680"
     CDS             complement(633..1805)
                     /locus_tag="PK976_04680"
                     /inference="COORDINATES: protein motif:HMM:NF025224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HRC67215.1"
                     /translation="MMNTRYRVCFFTIFLLFNGFSYYSIFSQPLSIEELAREKVYNSY
                     DEAFGDPWNCVVLDLSYQNLTFNSERVRNLNNLRVLHLAGNPNVDFEEAFTRKYFGSL
                     PYLQIIDLQENNLKYLPSGLSKFPYLNMFFAGKNQITEIPDYFCRFEYLQVLSLHHNR
                     IRTLPECLGSLDSLQTLDLSGNTSLQLDQVIPILKECRNLRQLYLSECNLDRLPVTIS
                     DLQSLHYLNLSENKLSQPPQYLFHLSHLQELYLSGNNITSLPDDIQFLENLEVLVLKN
                     NQLKTIPNTIKNLHKLVYLDISGNKLANVSDSLFFLKELKELHLASNQLSSLPAAIKE
                     LTQLEVLDVSSNKLTSLPEEVDYCNSLKYINLKGNSINKEKVQRMKDKWKTVQIVF"
BASE COUNT          603 a          342 c          341 g          614 t
ORIGIN      
        1 gattttcggg caccacactc accaacaagg attcggaatc aggcgtccat tgatatggag
       61 ctcccataca tgcatttaaa cgttgatgat agatcattct ggccattcct gtttcgagct
      121 cgattatcca taatgaaata tttctcccaa cggtattcgt aaatgctaac tttctgctgt
      181 cgggtgaaaa gctgacattg gcaatattca ggctatcggg gatatttttt aatgttactt
      241 ccttacccgt ggaaaggtcg atcaactgca ctccataaaa atatcttatc ctgcttggag
      301 catgagtttg cggatcgatt cttaatccgg ccaatcttaa ttctggctgg gctaattctt
      361 ctaaattctt gaaagccttg cgctcaaaca gcaacatgta tcgattatga taatcgatat
      421 aaacagccgg ctgaggcttt acatcaacta attgcaatat ctcgggtggt ggaagctgaa
      481 attttatctc ctcctgggca tagttctcca taaacatcaa taataaataa ggtaaaataa
      541 tcgtcaattt tttcataaag cattgagttt tttatttaca ttgaatctta ttgaacgaat
      601 tcatgtatta tcccttctcc ctatctgcat catcaaaaga caatctggac ggttttccat
      661 ttgtctttca tacgctgaac cttctcctta ttaatagaat tcccttttaa attaatgtat
      721 tttaatgaat tacagtaatc tacctcctct ggtaaactcg tgagcttatt ggaggataca
      781 tcaagcactt cgagctgagt gagttctttt attgctgcag gcaaagaact gagttgatta
      841 gatgctagat gcaattcctt caattctttc aaaaaaaata aagaatcgga tacattggct
      901 agtttattcc cagaaatatc gagataaaca agcttgtgga gattttttat agtattagga
      961 attgttttca attggttgtt tttcaacact aacacttcga ggttttctaa aaattgaatg
     1021 tcatcaggca gagaggtgat attattaccg gatagataaa gttcctgtaa atgagacaga
     1081 tgaaataaat attgaggtgg ctgcgatagc ttattttctg aaagattcaa ataatgcaat
     1141 gattgtaaat cgcttatagt aacaggaaga cgatcaagat tgcattcact taaataaagt
     1201 tgacgcagat tcctgcattc ttttaaaata ggaataacct gatcgagctg caaagatgta
     1261 ttgcccgata aatcgagggt ttgcagacta tcgagcgaac cgaggcattc gggcagagtt
     1321 cgaattctat tatgatgaag tgagagtact tgaagatatt caaatctgca aaaataatcg
     1381 ggtatttctg taatttgatt tttaccggca aaaaacatat tcaagtaagg aaacttcgat
     1441 aaacccgaag ggagatattt gaggttgttt tcctgcaggt cgattatctg caaatagggc
     1501 aaacttccaa aatactttcg agtaaaggct tcttcgaaat caacattagg attaccggct
     1561 agatgaagca ctcttaaatt attcagattt ctaacacgct cagaattaaa agtaaggttt
     1621 tgatagctta aatcgagcac tacacaattc catgggtctc caaatgcttc gtcatacgag
     1681 ttgtaaacct tttctctagc taattcttca atggataagg gctggctaaa gatagaataa
     1741 tacgaaaagc cattaaataa cagaaatata gtaaaaaaac aaaccctata tcgtgtattc
     1801 atcataaatt catttttcct gcgatcaata tgaataattt atcttgtata cgctcctttc
     1861 ggcttattct aaattatctg tccattgttt gctaaaactt
//
LOCUS       DAORPD010000333         1899 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_11314_length_1899_cov_2.695228, whole genome shotgun sequence.
ACCESSION   DAORPD010000333 DAORPD010000000
VERSION     DAORPD010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11314_length_1899_cov_2.695228"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="PK976_04685"
     CDS             <1..498
                     /locus_tag="PK976_04685"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC67216.1"
                     /translation="PCSGFPETLLEKGIITRIDSAWTKVPFFGWIPVPGFPMITVDTM
                     YNWFTANSIHPYAQLVVDQSGTPYSASYYSGNLEAIDEYSHSQNFPFVVSPNPVESEV
                     SLFSTQTGWSGKLFDYTGRLLKDDIKVRIGTTRVDVSGLKTGVYLLVLKNNRGEVKTI
                     KIQKR"
     gene            complement(500..>1899)
                     /gene="lysS"
                     /locus_tag="PK976_04690"
     CDS             complement(500..>1899)
                     /gene="lysS"
                     /locus_tag="PK976_04690"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HRC67217.1"
                     /translation="IGDIIWVKGFVFITQMGEITIHVKELELLAKSLRPLPIVKEKDD
                     KVYDAFQNPEQRYRQRYLDLIVNPHVRQIFIKRTQMVNSMRDFLNWKGYLEVETPILQ
                     PLYGGAAARPFKTYHNALDATLYLRIANELYLKRLVVGGFDGVYEFSKDFRNEGMDRF
                     HNPEFTQMELYVAYKDYYWMMDLVEEMVEKIALDLHSSTQIQVGDHVINFKRPWKRFT
                     MTGIIQEFTGLDISDMDETQLRQTATRLNIEITDAMGKGKIIDEIFGEKCEPYLIQPT
                     FIMDYPIEMSPLAHKHRSKTGLVERFEMICNGKEICNAFSELNDPIDQRQRFEAQLEL
                     RRRGDEESMVLDEDFLRALEYGMPPTAGLGIGIDRLAMIMTNSSSIQEVILFPQMRPE
                     KSQEVAPPSDLEDTGIPREWIPLVIQAGYTSKEALSQANPGSLFNQLGGLRKKNKIDI
                     PMPSLDEVKQWIANC"
BASE COUNT          520 a          401 c          401 g          577 t
ORIGIN      
        1 ccctgtagtg gatttccaga gaccttgctc gagaaaggca taatcactcg aattgacagt
       61 gcatggacca aggtgccatt tttcggatgg attcctgtcc caggcttccc aatgataacc
      121 gtagatacaa tgtacaactg gtttaccgct aattctatac atccttatgc tcagttggta
      181 gttgaccaat ctggtacacc ctacagcgca agttattatt cgggcaattt agaggcaatc
      241 gatgaatatt cacactcgca gaatttcccg tttgtagtaa gtccaaatcc ggttgaaagc
      301 gaagtttcat tattttcgac tcaaacaggt tggagcggta aacttttcga ttataccgga
      361 agattgctta aggacgatat taaagtgaga ataggaacga ccagagtcga tgtttcaggg
      421 ctcaagacag gggtttatct cttggttttg aagaataata gaggagaagt aaaaaccata
      481 aaaattcaaa agcgttgatt tagcagtttg caatccattg ttttacttca tccaacgaag
      541 gcatggggat atcgatctta tttttcttgc gcaagcctcc taactggttg aaaagagaac
      601 ctggatttgc ctggctgagg gcttccttgc ttgtgtaccc tgcctgaata accaggggaa
      661 tccattcacg aggtattcct gtatcttcca gatcgcttgg gggagcaact tcctgacttt
      721 tttccggtcg catttgaggg aatagaatca cttcctgaat agacgaagaa ttcgtcatga
      781 tcattgccag acgatctatt ccaatcccaa gaccagccgt aggtggcata ccatactcga
      841 gggctctcag aaaatcttcg tctaacacca tggattcttc atcgcctctg cgtctcagtt
      901 ctaattgtgc ttcgaaacgc tgacgctgat cgatgggatc attcaattcg gaaaaagcat
      961 tgcaaatttc ttttccgtta caaatcatct cgaatctttc caccaaacca gttttggaac
     1021 ggtgtttatg cgccagaggc gacatttcga taggataatc catgataaat gttggttgaa
     1081 ttaaataagg ttcacatttt tcgccgaaga tctcatctat gatttttcct tttcccattg
     1141 catcggtaat ttcaatattc aagcgggttg cagtctggcg gagttgggtt tcatccatgt
     1201 cggagatgtc gagacccgta aattcttgaa tgataccagt catggtaaat cgtttccaag
     1261 gtcgtttgaa attgataaca tgatcaccca cctgaatttg tgtggatgaa tgaaggtcta
     1321 aagcaatttt ttcgaccatt tcttcaacca ggtccatcat ccaataatag tctttatagg
     1381 ctacataaag ctccatctgg gtaaattcgg ggttatggaa cctgtccatt ccttcgttgc
     1441 gaaagtcttt cgagaattca taaacaccat cgaatccacc gacgaccagg cgcttcaaat
     1501 acagttcgtt ggctattcga agataaagag tggcgtcgag agcattatga taagttttga
     1561 aaggccgggc agcagctcct ccatataaag gttgaagaat aggtgtttct acctcgaggt
     1621 atcctttcca atttagaaaa tcccgcatcg aattgaccat ttgcgttctc ttaataaata
     1681 tttgccttac atgcggattg acgatcaagt cgagatatct ttgacgatat ctttgctcgg
     1741 gattctgaaa agcatcatat actttgtcgt ctttttcttt tacaataggg agtggtcgta
     1801 acgattttgc cagcagttcg agctctttca cgtgtatggt aatttctccc atctgggtaa
     1861 tgaaaacaaa gccttttacc cagataatat cgcctatgt
//
LOCUS       DAORPD010000334         1896 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_11342_length_1896_cov_2.126018, whole genome shotgun sequence.
ACCESSION   DAORPD010000334 DAORPD010000000
VERSION     DAORPD010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11342_length_1896_cov_2.126018"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="PK976_04695"
     CDS             <1..294
                     /locus_tag="PK976_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67218.1"
                     /translation="SWEFDGPADKTAVIESENLKIDFPYLSPAELKFTKGPSKHILSH
                     RTLCLNFLVFESTMLPAIKNKKHRVISIDEAIHLPLPRPIEKFLEELIVGQIP"
     gene            complement(481..1509)
                     /locus_tag="PK976_04700"
     CDS             complement(481..1509)
                     /locus_tag="PK976_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase family protein"
                     /protein_id="HRC67219.1"
                     /translation="MTSFDPNAIGIPNGNFLGLPYSNEEADIIIIPVPWDVTTSYHPG
                     TCQGPEAIIKASTQVDLFDFFLEQAWKNRIATLPLSEDILQLNEQLRPLAQQVIHLLE
                     TGMNANNPQILPLLEKINLGCEKMNQWVYKTSYYWISKGKTACVLGGDHSTSLGLMRA
                     LTKQYDGFGILHIDAHADLRKAYEGFIFSHASSMYNALELKSLEKLVQVGIRDVCEDE
                     MMLGYHDKRIKQYDGYLLAFETLNGKPFSEICEEIVKHLPSKVYVSFDIDGLNPELCP
                     HTGTPVPGGLSFDQVVLLLHILATSGRQIIGFDLVEVAPGSDDEWDANVGARILQKLC
                     NLTRLSIP"
BASE COUNT          582 a          385 c          364 g          565 t
ORIGIN      
        1 tcctgggaat tcgatggtcc ggctgataag acagcagtaa tagaatctga gaatttgaaa
       61 atcgattttc cttacctttc tcccgctgaa ttaaagttta caaaaggccc atcgaaacat
      121 atcctcagcc atcgtacctt atgtcttaat ttcctggtct ttgaatcgac catgttgcct
      181 gctattaaaa acaagaaaca tagagtgatt tcaatagatg aagctattca tttgcctttg
      241 cctcgtccga tagaaaaatt cctggaagaa ttaattgttg ggcaaatacc atgaaaacat
      301 ctatagggaa gaacgagaat aaaaaaattc gatgaacggg atgataattt tcttatttcc
      361 ctcacaagtg cctgttgcaa aatggaatgt gaaatctgtg ccctttaaaa attttggaga
      421 gataaggtat tatttcatga aggtttcagg attctgattc tttgaatttc aatcggcagc
      481 tcagggaatg gaaaggcggg tgaggttaca tagtttttga agaatgcgag ctccaacgtt
      541 ggcgtcccat tcgtcatcgg aaccaggagc aacctctacc agatcgaaac caatgatttg
      601 ccggcccgaa gttgccagaa tatgcagcaa tagtacaacc tgatcgaatg aaagtccacc
      661 cggaacgggt gttcctgtgt gggggcagag ttcgggattt aatccatcta tatcgaaact
      721 gacatacact ttcgaaggca gatgcttcac gatttcttcg cagatttcgc tgaagggctt
      781 tccattcaaa gtttcgaagg ctagtaaata tccatcgtat tgcttgattc ttttgtcgtg
      841 atatcccagc atcatttcat cttcacaaac atcgcgtatc cccacttgaa cgagtttctc
      901 aagcgatttc aactccagtg cattgtacat tgatgaagca tggctaaaga taaaaccttc
      961 gtaagccttt cttaaatcgg catgagcgtc gatgtgaagt attccaaaac catcgtattg
     1021 cttcgtcaac gcacgcatca atcctaatga agtgctatga tcacctccga gcacacaggc
     1081 tgttttaccc ttcgaaatcc agtaataact ggttttataa acccattggt tcatcttttc
     1141 acatccaaga ttaatttttt cgagtaaggg caatatctgg gggttatttg cattcatgcc
     1201 agtttctaaa agatggatta cctgctgggc aagcggccgc aattgttcat tgagctgcaa
     1261 aatatcttca gatagaggca gagtggctat tcgattcttc catgcctgtt cgagaaagaa
     1321 atcaaataaa tcgacctgag ttgaggcctt tatgattgcc tcaggcccct ggcaagtacc
     1381 aggatgataa gaagttgtaa catcccatgg aacaggaata atgatgatat cggcttcttc
     1441 gttgctataa ggcaaaccta agaaatttcc attgggaatg ccaatggcat tgggatcgaa
     1501 tgaagtcata taattggtat tacttatgca aaattagtga tttcgatata agcctatatt
     1561 atacctcttc tcatctgaat attcgaaaat tcgtgttatt tataaattca aaaatggatc
     1621 gtagtttatt gactattagc ttgtagcttt atagaaatga tggcaactgg cattctctct
     1681 tgctaattta gtccaccgat tcacactgat cgtctgcggt tatctgcaag aactctttat
     1741 tttcttactg attgacgcag attaaatccg ctgattataa caaacaacaa acaactacaa
     1801 gatttcaaat tacaagcaaa tttcaacgaa caactgacaa ggaacaactt acaactacaa
     1861 catttcaaat tacaaattcc aaactccaaa tttcag
//
LOCUS       DAORPD010000335         1893 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_11376_length_1893_cov_1.928183, whole genome shotgun sequence.
ACCESSION   DAORPD010000335 DAORPD010000000
VERSION     DAORPD010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11376_length_1893_cov_1.928183"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(141..>1893)
                     /locus_tag="PK976_04705"
     CDS             complement(141..>1893)
                     /locus_tag="PK976_04705"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRC67220.1"
                     /translation="DQVIKIPLDYSALLKNQPIPDTSRRTSKGGFLTYTVKEKETLYA
                     ISRKFGVTIADIIEWNPQVKEGLKAGEEILIGKPGIKPQERGFIEYKIQKHESLYGIA
                     RKNRISIDSLKMFNQGLTENIKEGQIILIPKRRAASYITHVAEPKENLQKIADKYEVG
                     EEELKKANPHIDKKIEENAPIKIPIQSENKKEHEMAQLPAETENIASTSLCNPDYKFK
                     HEEFKIAIIVPFMLNTPLNVRSQSSAYGKKPGDSPLFKYLPFYEGALLALDSLKKIGF
                     NAKVYVYDLGNDMSGTHRLLQKPEMKSMDLIISLAFSKNFELIADFAKRHKIPTINAI
                     SKRDEILSNNEYIIKPYPSEDFQAEAVAQFLSSRPGKQNIIILRNNKNLYTNTIDKLL
                     TMLDQHLQEGHFTKGSRIHFLNDSVTKVKSAIKPGYSNYLIVLSENEAFNINLLRNLR
                     TLNDTLRFSLIGMPSWANMKNAEISSLIQNNVHFLSPFMVDYYDEKVQDFLYKYRDRY
                     LAEPNELAFTGFDITWFFCNALMNYGKDFRECLPYLRISNIQSSYRYKWLKPNGLVNT
                     FWNIYQYIGYRPVIVNL"
BASE COUNT          546 a          333 c          373 g          641 t
ORIGIN      
        1 aaaacttaat cttcatctcc tctgaaaaag ttgcatttat tagttgaatt tagtatatgt
       61 tttatagaat tttacaaaaa aaagaaaacg ttatgcaaag gtataaatag ccttttcaat
      121 aaatagacta aaaaataaat ttataggttg acaatgacag gccggtagcc aatatattgg
      181 taaatgttcc agaaagtatt caccaaacca tttggtttta accatttata gcgataactg
      241 gattgaatgt tggatatacg caaataaggt aggcattcgc ggaaatcttt tccataattc
      301 atcaaggcat tgcaaaaaaa ccatgtgatg tcgaatccag taaaggcgag ttcgttgggt
      361 tcggccaagt aacggtcacg gtatttatat aaaaaatctt gaaccttttc atcgtagtaa
      421 tctaccataa aaggagaaag aaaatgcaca ttattctgaa tgagtgaaga aatctcagca
      481 tttttcatat tggcccacga aggcataccg atgagggaaa atctcagggt atcgttcaaa
      541 gtacgcagat tccgtaaaag attgatattg aatgcttcat tttctgaaag tacgatcaaa
      601 taattggaat aacccggctt gatagctgat ttaacttttg ttacactatc gttgagaaag
      661 tgtattcgac ttcccttggt aaaatggcct tcttggagat gctgatcgag cattgtgagg
      721 agtttatcga tggtattcgt gtataaattt ttattgttgc gcagaatgat gatgttttgt
      781 ttaccaggcc tcgagcttaa gaattgggca actgcttcgg cctgaaaatc ttcggatgga
      841 taaggcttaa tgatgtattc gttgttcgac aatatttcat ctcttttaga tatagcattg
      901 atagtaggaa ttttatggcg ttttgcaaag tcggcaatta gctcgaaatt cttgctgaaa
      961 gccaggctaa ttatcaaatc catggatttc atctcgggtt tttgcaataa cctatgggtt
     1021 ccactcatat cgtttcccag atcgtacaca tacacttttg cattaaaacc tatcttcttg
     1081 agtgaatcaa gagccagaag agcaccttcg taaaatggca gatacttgaa caagggagaa
     1141 tcacctggct ttttaccata agctgagctc tggctacgaa cattcaaggg agtattcagc
     1201 atgaaaggga caatgatggc aattttaaat tcctcgtgtt tgaatttata atcgggattg
     1261 cacaatgatg ttgatgcaat attttcggtt tcggcaggaa gctgagccat ttcatgttct
     1321 ttcttgtttt cggactggat aggaattttg ataggagcat tttcttcaat ttttttatcg
     1381 atgtgaggat tggctttttt aagctcctcc tctcctactt catatttgtc ggcaattttt
     1441 tgtaaatttt ccttcggttc ggccacatga gtaatataag aagctgctct tcgtttagga
     1501 atgaggataa tttgtccttc tttgatattt tcggtaaggc cctggttaaa cattttcaac
     1561 gaatctatac tgatgcggtt tttacgggcg attccataaa gactttcgtg tttctgtatc
     1621 ttatactcaa tgaatcctct ttcctgaggc tttatgcctg gcttcccaat caatatctcc
     1681 tcacctgcct tgagtccttc cttcacctga ggattccatt cgatgatatc agcaatagtt
     1741 actccaaatt tccggctgat ggcatataat gtttcttttt cttttactgt ataggtaaga
     1801 aaacctcctt tagaggttct ccttgacgta tcgggaattg gctgattttt cagcaatgct
     1861 gaataatcga ggggaatttt gattacctga tcc
//
LOCUS       DAORPD010000336         1890 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_11397_length_1890_cov_3.014714, whole genome shotgun sequence.
ACCESSION   DAORPD010000336 DAORPD010000000
VERSION     DAORPD010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11397_length_1890_cov_3.014714"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1890
                     /locus_tag="PK976_04710"
     CDS             <1..>1890
                     /locus_tag="PK976_04710"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HRC67221.1"
                     /translation="DVTQIPAFNGTTGSHATDLRYAEYTGDNLPEVYYGRFSANNLNE
                     LQPQIDKTLQYEQYSFPSENFLGEAVMIAGDDAGHMTYSNGQITYGTTNYFNLQHGIL
                     SHTYFQPEPTGGNYSQNIIQNISNGVGYANYTAHCSASGWANPSFTISNIPSLQNQDK
                     YPLMVGNCCQSSMYNTTCFAEEIVRAANKGAVGYIGGSDYTYWDEDYWWGCGFKTVST
                     NPPYDPNHLGAYDVTFHDHGEPSSSWYVTQGQMFVGGNMAVQQSNSSMKAYYWEIYCL
                     MGDPSLMIYYGIPQPIAANYQNTLLIGTDNLTITTEQYAYAALSFEGNLLATGMAEAN
                     GTITLNFSPLTNVGNATLIITKQNRKPHIGTIQVIPATGPYLIVQNFTVNDSLGNNNG
                     HADYGEDVFLNVTYKNVGVQSAVNATSNLFTADTNVILTDTSCFMQIVEPNSTFVVRN
                     AFRINIKPKVDDQYNVPLTFSTFDTANIWNTNKYLKLYAPILEIGNLIIDDAITGNGN
                     GVIDPGETVNLILQVSNTGGAVALNAKCSIDIDPLYSNYLMALNPLVNLGEVGIGENK
                     QAFFSVMANIITPPNTTLPLIFKFTAGQMNQYEAENTKEVIIGGPATILMTNGSTNTC
                     NGAFY"
BASE COUNT          576 a          408 c          361 g          545 t
ORIGIN      
        1 tgatgtgact caaattccgg ccttcaatgg aacaacagga agtcacgcta ccgatttgcg
       61 ctatgccgaa tacacgggtg acaatctgcc cgaggtttat tatggaaggt tttcggcaaa
      121 taatctgaat gaattacaac cccaaattga taaaacttta caatatgaac aatattcctt
      181 cccttccgag aattttttgg gcgaagcggt aatgatagca ggtgatgatg ctggccatat
      241 gacatatagc aacgggcaaa tcacctatgg aacaacaaat tatttcaatc ttcagcatgg
      301 tatcctgtct catacttatt ttcaacccga acctactggc ggtaactatt cgcaaaacat
      361 cattcaaaat atttcgaacg gtgtaggata tgccaactat acggcacatt gcagcgcttc
      421 gggatgggca aatccaagtt ttactatttc gaacattcct tccttacaaa atcaagataa
      481 atacccattg atggtaggaa actgttgtca atcctccatg tacaacacca cctgttttgc
      541 agaagaaatc gtaagagccg ccaataaggg tgctgtgggg tatattggtg ggtccgacta
      601 cacttattgg gatgaggatt actggtgggg atgtggtttc aaaaccgttt cgaccaatcc
      661 tccttatgat cccaatcatc tgggagctta cgacgtaacc ttccatgatc atggagaacc
      721 tagtagtagc tggtatgtta ctcagggaca gatgtttgta ggcggaaaca tggccgtaca
      781 acagtccaat tccagcatga aagcatacta ctgggaaatt tattgcctga tgggcgatcc
      841 ctccctgatg atctattatg gaatccctca acccattgct gcaaattatc aaaatacatt
      901 gctcatcggt acagataatc tcaccattac taccgagcag tatgcctatg cagctctctc
      961 tttcgaagga aatctgcttg cgacgggtat ggctgaagcc aatggcacta taactcttaa
     1021 tttttctcca ctcacaaatg ttggaaatgc tactcttatc attactaaac aaaaccgtaa
     1081 acctcacatt ggcactatac aggtaatccc cgctacaggt ccttatctga tcgtacagaa
     1141 ctttactgtg aatgattcat taggcaacaa caatggacat gccgattatg gagaagatgt
     1201 cttcttgaat gttacttaca aaaatgtagg tgttcagtca gctgtgaatg ccacatcaaa
     1261 tctctttacg gctgatacga atgtcatact gactgatact tcttgtttta tgcaaatcgt
     1321 tgagccaaat tccactttcg ttgttcgcaa tgctttcaga atcaacatta aaccgaaagt
     1381 tgatgatcaa tataatgttc ctctcacgtt tagcactttt gatacagcca atatctggaa
     1441 caccaataaa tacttgaagc tctatgcacc tattcttgaa attggaaatc ttattatcga
     1501 cgatgcgatt acaggcaacg gaaatggagt aattgatcca ggtgaaactg tcaacctcat
     1561 attgcaagtt tcgaacactg gtggagcagt agcccttaat gcaaaatgct ctattgatat
     1621 cgatcctttg tattcgaact acttaatggc tttaaatcca ctggtaaatt tgggtgaagt
     1681 tggaattgga gagaacaaac aagccttttt ctccgttatg gccaatatta tcactccccc
     1741 caatactacc ttacctttga tctttaaatt cactgctgga caaatgaatc aatatgaagc
     1801 agaaaatacc aaagaagtca ttataggcgg acccgcaaca attttaatga cgaatggttc
     1861 aacaaacacc tgtaatggtg cattttatga
//
LOCUS       DAORPD010000337         1883 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_11468_length_1883_cov_2.253282, whole genome shotgun sequence.
ACCESSION   DAORPD010000337 DAORPD010000000
VERSION     DAORPD010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11468_length_1883_cov_2.253282"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(148..1659)
                     /locus_tag="PK976_04715"
     CDS             complement(148..1659)
                     /locus_tag="PK976_04715"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRC67222.1"
                     /translation="MPDHIPIIKWAAGAPVALPDDVDFNTNTLIDLVDAHNLSGQFLS
                     RIRNNNPKWVTYDLVEGLNDLFFMTTTQVARNISAFTQLRQQLKFKKTNIIVIKGIST
                     YVLSDESEIMHAGDIDLLSDNTNNVVQTLLDTGYRQTRTPFMHEIGEFTKDSTEFDVH
                     DYFPVYGYSKTLLESDLSPLHQNEEWQQQGYSFPHNKITFFDMFKSANHGTRPETEPI
                     VVPDANMLAMIISAHAFMNYTNIWSISHREKACVRLGEIIDLFALSRHASFNPNRFIS
                     YVDYFNAHDAVEWAASTAVSVFGKNPLPISVSNNLGQPVSATRFPRCLWWNFWASLSS
                     EPDELLKTKWLSMEWLIQRIGANHIPVSQESEKSYSTVNSDFYSPLRRYITQQPEPIP
                     LVIQIRRSEKGLILNLQISSSFEFDTERIRFDLGSIACEWIYDVGRNCTKTVGYPMDL
                     SFRCNASGYEIIAKLDLEEIVLPMKIVGLCIGVMRQAGLNVLESILIPLELEF"
     gene            complement(1668..>1883)
                     /locus_tag="PK976_04720"
     CDS             complement(1668..>1883)
                     /locus_tag="PK976_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67223.1"
                     /translation="LAYFDMDLYQPTKSCLEIIQPYLVKGSILAFDELLHPEFPGETL
                     ALKEVLDLRKYKIERFPTAPYPSYIVI"
BASE COUNT          572 a          371 c          369 g          571 t
ORIGIN      
        1 tcgtgatgtt tcttgcgact gagacgttcc tccagaatag ctactcgtat ttgcccatct
       61 atcattaagc aagcggaggc atcatgagcg caattaatac caagaataac ctgtgactga
      121 agatccattt ttaatttcct ttgattatta aaattccaat tcaagaggaa taaggatgct
      181 ttccaaaaca ttcaggccag cttgtctcat cacccctatg caaagtccaa caattttcat
      241 tggtaaaaca atctcttcca aatcaagttt cgcgataatc tcataacccg aagcattaca
      301 tctgaatgat aagtccatag ggtatccaac tgtctttgta caatttctgc ccacatcata
      361 aatccactca catgcaatac ttcctaaatc gaaacggatc cgctctgtat caaattcaaa
      421 acttgaagat atttgcaggt taagtattag ccccttttcg cttctgcgga tttgaatgac
      481 taatggaata ggttcaggtt gctgggttat atatcgtctg agcggtgaat aaaaatctga
      541 atttactgtg gagtaacttt tttctgattc ttgtgaaaca ggaatatggt tagcgccgat
      601 tctctgaatc aaccattcca tggaaagcca tttggttttc aggagttcat ccggctctga
      661 tgataaactg gcccaaaaat tccaccacag gcatctggga aatcgagttg ctgatactgg
      721 ttgtccaaga ttattagata cagaaatggg cagcggattt ttaccaaata cagaaacagc
      781 cgtagaagct gcccactcaa cagcatcatg agcattgaaa taatctacgt aacttataaa
      841 cctattagga ttgaacgatg catgtcttga gagggcaaaa aggtcaataa tttctccaag
      901 tcggacgcaa gccttctctc gatgcgaaat cgaccaaata ttggtgtaat tcatgaaggc
      961 atgggcgctt ataatcatgg ctaacatgtt tgcatctggt actacaatag gctcagtttc
     1021 tggtcgtgta ccatgatttg cggacttaaa catgtcgaaa aaggtaattt tattatgggg
     1081 aaatgaatag ccttgttgtt gccattcctc attttgatgc agaggagaca agtcagactc
     1141 cagtaatgtt tttgagtacc cataaacggg aaaataatcg tgaacatcga attctgttga
     1201 atctttcgta aattcaccta tttcatgcat gaaaggagtt cttgtttgtc tataacctgt
     1261 gtcgagtaaa gtttgcacaa cattattggt attatcactt agtaaatcaa tatctcccgc
     1321 atgcataatc tcgctctcat cagacaggac ataagtcgat atacctttga tgacaataat
     1381 attggttttt ttgaatttca attgttgtct gagctgagta aacgctgaga tatttcgggc
     1441 aacttgggtt gttgtcataa aaaacaaatc gtttaaaccc tcaacaagat cataagtcac
     1501 ccatttaggg ttattatttc gtattcgcga caaaaactgc cccgagagat tgtgcgcatc
     1561 tacaaggtcg attagggtat tcgtattgaa atcaacatca tcgggcaatg caacaggagc
     1621 accagcagcc catttaatta ttgggatgtg atcaggcata ggttttctta aattactatg
     1681 taagagggat atggggctgt aggaaatctt tctattttat acttcctgag atctaaaacc
     1741 tcttttaagg ccaatgtttc gcctggaaac tcaggatgta gcagttcatc aaatgccagg
     1801 atgctaccct tcacaagata aggctgaatt atttccagac aactcttggt gggttggtaa
     1861 agatccatat caaagtaagc gag
//
LOCUS       DAORPD010000338         1876 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_11539_length_1876_cov_2.363537, whole genome shotgun sequence.
ACCESSION   DAORPD010000338 DAORPD010000000
VERSION     DAORPD010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11539_length_1876_cov_2.363537"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="PK976_04725"
     CDS             <1..508
                     /locus_tag="PK976_04725"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRC67224.1"
                     /translation="ISFITIQYFEPHSIYTKKLAERGELITHHKDKAVLSMMKVTDLL
                     ETNFKTVFPDATLGEFVKVVASSERNVFPVVDKENNFLGVVFINDIRHILFQQELYHK
                     IFVKDLMFMPDVKVSPDDTMEDVAKLFQQTAHYNIPVIKDGKYLGFVSRANVFSNYRK
                     LLKEFSED"
     gene            622..>1876
                     /locus_tag="PK976_04730"
     CDS             622..>1876
                     /locus_tag="PK976_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HRC67225.1"
                     /translation="MFKNHPKGLLVLAITNMGERFGYYTMLAILTLYMQAKFGLTSAT
                     TSMIYGTFLALVYFLPILGGVIADKALGYGKTILLGILVMFIGYLLLAIPLESNAVGL
                     ATMIFSLVLIALGTGFFKGNLQALVGKLYDDPRYGKNRDIAFSIFYMFINIGAFFAPS
                     AANYINNTVLRSADFTYDASIPKNYVILNDEKNAIDKNTFILKYISPEEQKLSGKEFE
                     SVLNKAKKEHKAIFTDDIEFALFRLKAAGLYQYTQSGKITDSSMMISSHEYELLQSRD
                     PKDAQDKADVITKIQAIHVTDPNLGGGPNPDDFARNFGKHYVSHSLSTSYNLAFGVAC
                     ISLIISVLIFLLFKKTWKAVDKTVKQEIAEAKITGKEVKMMPKEEVRERMIALFLVFF
                     VVIFFWMSFHQNGLTLTFFARDYTMS"
BASE COUNT          552 a          366 c          372 g          586 t
ORIGIN      
        1 tatttctttt atcaccatcc aatatttcga acctcattcc atctatacaa agaagctggc
       61 cgaacgtggc gaactgatta ctcatcataa ggataaagct gtactctcga tgatgaaagt
      121 taccgatttg cttgaaacca atttcaaaac tgtttttcct gatgctacct tgggagaatt
      181 cgtaaaagta gtagcaagtt ccgagcgtaa tgttttccct gtggtagata aagagaataa
      241 ttttttggga gtagttttca tcaacgacat tcgacatatt ttgtttcaac aggaactata
      301 tcacaagatc ttcgtaaaag atttgatgtt tatgcccgat gtaaaagtaa gccccgacga
      361 tactatggaa gatgtggcta aactgtttca acaaaccgcc cactacaaca ttcctgtgat
      421 aaaagatggg aaatatctgg gcttcgtttc cagagccaat gtcttttcca attatcgtaa
      481 attgcttaag gagttttcgg aagactgatc atctgttttc tttgttgttt tatcgtagat
      541 ttaattttct gtttttaaaa aggatgtata tttgctgcca aagtgaaccg aatgtttaat
      601 taaatatcta aatctaagta tatgttcaag aatcatccca aaggattatt ggttttagcc
      661 attaccaaca tgggcgagag atttggctac tacaccatgt tggctattct taccttgtat
      721 atgcaggcca agtttggttt gacttcggcc acaaccagca tgatttatgg aacctttctg
      781 gctcttgttt atttcctccc cattttagga ggagttatag ctgataaggc actgggttat
      841 gggaaaacga ttctgttggg catcctggtc atgtttatcg gatatctgtt gcttgctatt
      901 ccactcgaaa gcaatgcagt aggattggcc acgatgattt tttcgctcgt tcttattgct
      961 ttgggtactg gatttttcaa gggaaattta caggcactcg taggcaagct ttacgatgat
     1021 ccccggtatg gaaagaatag agatattgca ttttcgattt tttatatgtt tatcaatatt
     1081 ggggccttct ttgctcccag cgcagcaaat tatatcaata ataccgtttt gaggtcggcc
     1141 gattttacct acgacgcttc catacctaaa aactatgtca ttctcaatga tgaaaagaat
     1201 gccattgata agaacacatt tattctgaaa tatataagcc ccgaagaaca aaagctgtcg
     1261 ggaaaggaat tcgaatccgt cttgaacaag gcaaaaaagg aacataaagc gattttcacc
     1321 gatgatatcg aatttgccct tttcagattg aaagccgctg gcctttatca atatactcaa
     1381 tcgggcaaga ttaccgattc ctccatgatg attagcagcc atgaatacga attgttacaa
     1441 agccgtgatc ccaaagatgc tcaggacaaa gccgatgtaa taaccaaaat acaagctatt
     1501 catgtaacag acccaaattt ggggggtggc ccaaaccccg acgattttgc tcgcaatttt
     1561 ggcaagcatt atgttagtca ttcactttcc acctcttata atctggcctt tggcgtagct
     1621 tgcatcagtt tgatcatttc cgttctcatc ttcctgctgt tcaagaaaac atggaaagca
     1681 gtagataaaa cggtaaaaca ggaaatcgcc gaagctaaga taacaggcaa agaggtaaag
     1741 atgatgccaa aagaagaagt gagagaacga atgatcgctc tgttcttggt atttttcgtg
     1801 gttatattct tctggatgtc gttccatcaa aacgggttaa cgcttacctt ctttgcccgc
     1861 gattatacca tgagca
//
LOCUS       DAORPD010000339         1869 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_11610_length_1869_cov_2.237596, whole genome shotgun sequence.
ACCESSION   DAORPD010000339 DAORPD010000000
VERSION     DAORPD010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11610_length_1869_cov_2.237596"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(243..>1869)
                     /locus_tag="PK976_04735"
     CDS             complement(243..>1869)
                     /locus_tag="PK976_04735"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC67226.1"
                     /translation="YDLQTNSTLGNRFYMYPEGQMVHVWTIGMTPTSYPDRGTGYNYW
                     DGTSWQPAPTSRIESSRTGWPGYAPAGANGEAVIAHRSSDLLFSYRENRFSGSWIQTP
                     IAGPTGTEPAWPRMIASGPDNQYLHYIVNSYTEYNGQGMALLYSRSLDGGQTWEIQNA
                     IIEELGPEYYYEIGADAYVMASRGETVAYLIGRPWMDLVLMKSNNNGETWHKTVIWQH
                     PYPFYDFTYVTDTFYCVDNSIALTIDKDYMVHVAFGINKVQGNSDGTYYLYPYVDGIG
                     YWNESRPPFSDNKMALCPYGFEGSEMIENYNLIGWSQDLNGNGQLDFQSDLLYYRSIG
                     LSTMPSIYVDDQDNVYVAYASTTEGYDNGTNNFKHIWMRVASNKGELWSGKFIDIMSD
                     ISHIFDEGIWPQLGQLSDDNVYLVYNVDPHPGLAVDGDHDPVDNHQLFVSIPKNQLGV
                     GIHDIELTNISKPYPNPASDKVFVSVDQKAAANVSVELTNLAGQIVKRCDYGNKTAGT
                     HLLSINTSSLKSGYYLLTVYSGTQTVTYPVLIQ"
BASE COUNT          565 a          374 c          383 g          547 t
ORIGIN      
        1 aaagccatta acaagaaaat cgttcaaagt gcttgaaaaa tttatcatat attcaatttt
       61 cttgttaatt tcgttggtag ggtgtctttt ttgaaattca ttaaaaaaac cctgtcaaag
      121 aatcccaaaa ttggtgaatt ttgataacct ttgctctgac agggttttgt taaaagctga
      181 attcggtcag agtattctat gatgaaagaa tttcatcgag aataaccgcc tcttgatatg
      241 gtttactgaa taagaacagg ataagtaacc gtttgagtac ccgaataaac tgtcaataaa
      301 taataccctg attttaaaga agatgtatta atggagagca aatgagttcc ggcagtttta
      361 tttccgtaat cacaacgttt tacgatttga ccggcaaggt tggtaagttc tactgaaaca
      421 ttagctgcag ccttttggtc gacagatacg aagactttat cggaagcagg attggggtaa
      481 ggtttcgaaa tgttagtcag ttctatatca tggatgccca caccgagctg atttttagga
      541 atagatacaa acaattggtg attatcaacc ggatcgtgat ctccatcaac agcaagacct
      601 ggatgcgggt caacattgta aaccaaatac acattatcgt cggagagttg ccctaactga
      661 ggccagattc cttcgtcgaa gatatgcgaa atgtcgctca taatatcgat aaatttgcct
      721 gaccataatt cacctttatt agatgctacc ctcatccaga tatgtttgaa attattggtt
      781 ccattatcat aaccttcggt agtagaggca taagccacat aaacattatc ctgatcatcc
      841 acgtaaatgc ttggcatagt agaaagtcca atagacctat aatataatag atcactctga
      901 aaatctaatt gtccattacc attcaaatcc tgactccagc cgataagatt gtaattttct
      961 atcatttccg aaccttcgaa gccataaggg caaagagcca ttttgttatc agaaaaggga
     1021 gggcgagatt cattccagta tccgataccg tctacgtaag gatataaata ataggttcca
     1081 tcactatttc cttgaacttt attgattccg aaggctacat gtaccatgta gtccttatcg
     1141 atagtcaagg caatggagtt gtccacacaa tagaaggtat cagtgacata ggtaaaatca
     1201 tagaatggat agggatgttg ccagatgacg gttttgtgcc aggtttcgcc attattgttt
     1261 gatttcataa gaacgaggtc catccatggt ctcccaatga gatatgctac ggtttctccc
     1321 cgcgatgcca ttacgtatgc atcggctcct atttcgtaat aatattcagg gcctaattct
     1381 tcaatgatag cattttggat ttcccaggtt tgccccccat ccaatgaacg agaataaagc
     1441 aaagccatgc cctgaccgtt atattcggta tagctattta ctatatagtg gagatactgg
     1501 ttatcagggc cagaagcaat catacgtggc caggcaggtt cggttcctgt aggaccagct
     1561 atgggagtct gaatccacga gcccgaaaaa cggttttctc gataagaaaa aagaagatcg
     1621 gatgatcgat gggcaatcac ggcttctccg ttggctcctg ctggtgcata tcctggccag
     1681 cctgtacgcg aactttcgat acgactcgta ggtgctggct gccatgaagt accatcccaa
     1741 taattataac cagttcctct gtcgggataa ctggttggag tcattcctat agtccaaaca
     1801 tgaaccatct gaccttcggg atacatgtaa aagcgattgc ccaaagtgga gttagtttga
     1861 agatcatat
//
LOCUS       DAORPD010000340         1866 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_11638_length_1866_cov_2.214246, whole genome shotgun sequence.
ACCESSION   DAORPD010000340 DAORPD010000000
VERSION     DAORPD010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11638_length_1866_cov_2.214246"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..943)
                     /locus_tag="PK976_04740"
     CDS             complement(<1..943)
                     /locus_tag="PK976_04740"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRC67227.1"
                     /translation="MKNTPIDYQIVRDCISKNGLISVGKASIREIKKLVDDIEQASGQ
                     RFIRMELGNPGLPPAAPGVKAQIEALEKGIASEYPDIQGIPPLKKEISRFLKLFVNID
                     VDPQYCFATVGSMQGSFASFLTVNRMTDGKNKTLFIDPGFPVHKQQVKVLGQPFKSFD
                     VYDYRGERLRDKLEEELADGTVASLIYSNPNNPAWFCFNEQELQIIAEVATKHDVVVI
                     EDLAYFGMDFRKDYGQPGQPPYQPSIARFYDKYILLISSSKIFSYAGERIACLIISPK
                     LYHTNTPDLKKYYGTDSFGHAFVFGTLYTLSSGTAHSP"
BASE COUNT          598 a          340 c          377 g          551 t
ORIGIN      
        1 ggggtgaatg tgctgttccg ctgctaagag tatataatgt accaaaaaca aaggcatggc
       61 caaaactgtc ggtaccgtaa tactttttta aatctggagt atttgtatga tataactttg
      121 gtgaaataat gagacatgcg attctttctc ctgcataact aaaaatcttg gaactcgata
      181 tcaagagaat gtatttatcg tagaaacgag ctatgcttgg ttgatatgga ggttggccgg
      241 gctgaccata atcctttcta aaatccatcc caaaataggc taaatcttca atgaccacca
      301 catcgtgttt ggttgctacc tcggcaatga tttgtagttc ttgctcgttg aagcagaacc
      361 atgcaggatt attgggattg gaataaataa gggaagccac cgtgccatcg gccaattcct
      421 cttccagttt atctcgtagt ctttcacctc tataatcata aacatcgaac gatttgaaag
      481 gctgtcccaa aactttaacc tgttgtttat gaactggaaa ccccggatca atgaataagg
      541 tcttgttttt accgtcggtc atgcggttaa cggtgagaaa agatgcaaaa ctaccctgca
      601 tgctgccaac agtcgcaaaa caatattgtg ggtcgacatc aatatttacg aaaagcttta
      661 agaatcggga aatttccttt ttaagcggag gaataccttg gatatcaggg tattcggagg
      721 caataccctt ttcaagagct tctatctggg cttttactcc aggcgctgca ggaggtaaac
      781 ccggattacc caattccata cggataaacc tttgaccact ggcctgttca atatcatcaa
      841 ccagtttctt gatttcacga atggatgcct ttcctaccga aatcaatcca tttttcgaga
      901 tacaatctct aacaatttga taatcaattg gagtattctt cataatataa atagttttgc
      961 aaggctaaaa tatgcaataa attcataact gacaatcaca aaaacaattc atcccatttc
     1021 tctaagggaa ctttccatag cagaagcgca gaaatacgct acaagcgact taaattctca
     1081 aggttcgagg ctgaagatag ccatagaagg actaagtttt gaaaaactcg gggatgacaa
     1141 aatgggaacc atcattttcg agccaaggtc agctttattt ttttatggat tgaatttttg
     1201 aacatgttta atgtttccaa catcgatgaa tttgaatttc gaattgatct cgaaaggtaa
     1261 atcaggtggc aggataaatc gaaagcgagc ccgcttgccg ggttctgagt tggaagtaat
     1321 tgtttcctaa aagccttttc gataatggcg gaaattaccg ataataattc caaaagtaaa
     1381 taagcatgat gaatgtgaaa tgcattggta tgtcaggaag tataaaacag aaaatctatt
     1441 ccgcgattag atacgatgag gagtaaggtc aaatttgaat gaattccttc ttcgatcaat
     1501 attcgtgaac tctggggata gggagattcg acgagttata aattcacatc acgaacgatt
     1561 tgaattttac cttcttttaa atacaggtta caagctcgaa agcgccaaat ctgccgagaa
     1621 agaattatca acaataagaa caggttctta tttctttcgg tcaaagtcaa ttcccgctcg
     1681 taaaatattg aattatagta gatatatttt tcatgctatg attctatttt atgaatattt
     1741 tatccagaaa aaatcaaatg ggtgtaaggc aagggggaga tgaagatcga taggtttatt
     1801 ggtgaaaagg tctataggag ccacgacgaa ttcaggccat aagaattcgc ccgttgtatt
     1861 ggcaag
//
LOCUS       DAORPD010000341         1865 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_11650_length_1865_cov_2.075691, whole genome shotgun sequence.
ACCESSION   DAORPD010000341 DAORPD010000000
VERSION     DAORPD010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11650_length_1865_cov_2.075691"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1114)
                     /gene="mnmE"
                     /locus_tag="PK976_04745"
     CDS             complement(<1..1114)
                     /gene="mnmE"
                     /locus_tag="PK976_04745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HRC67228.1"
                     /translation="MESNLNTLSEMFAMESTICAPATPQGTGAIAIIRLSGPNTFRII
                     EKIFQPAKSSLKIKDIKSHTVHLGNFHNEDEIIDEGLITVFRAPHSYTGQDVVEISCH
                     GSRYIQNTLLSLLMKHGAVMARPGEFTLRAFLNGKMDLSQAEAVADLVNAEAHMAHQL
                     AIRQMRGGFSSEIQQMRQKLIDFTSLIELELDFPEEDVEFANREHLLELLGEIRQKLH
                     NLIESFRYGNVIKHGIPVAIIGKTNVGKSTLLNALLNEERAIVSEIPGTTRDSIEDTM
                     VIEGITFRFIDTAGLRQSKEPIESLGIERTYEKINQARIILYVFDVNETTIEEIKENL
                     CDFQHHLTNPDKKFILVANKIDLLIESPRQFADFVEL"
     gene            complement(1415..>1865)
                     /locus_tag="PK976_04750"
     CDS             complement(1415..>1865)
                     /locus_tag="PK976_04750"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HRC67229.1"
                     /translation="VALNISDLEQVKQNFAKLMAIEDAVGVLFQPMLSGTELFIGANW
                     ELKFGHLILCGIGGILIETLKDFSTALAPLSHDEAVSMIHNLKSYSLFKGVRGKEGIN
                     EGIFTDILLRVSSLLQAAPEIREMDLNPLLGNSKSMTTVDARIRIEK"
BASE COUNT          473 a          407 c          369 g          616 t
ORIGIN      
        1 cgagttcgac gaaatcggca aactgccgtg gcgattcgat gagaaggtct attttattgg
       61 ctaccagaat aaatttttta tcagggttag ttaaatgatg ttggaaatcg caaaggtttt
      121 ccttgatttc ttcgatagta gtttcattta catcgaaaac ataaaggatg atacgggctt
      181 gattgatttt ttcgtaagtt ctttcgatgc ccagcgattc gatgggttct ttgctttgac
      241 gcaacccggc cgtatcgata aagcggaatg tgattccctc tatgaccatg gtatcctcaa
      301 tgctatcgcg tgtagtaccg ggaatttcgg atactatggc tctttcttcg ttcaataagg
      361 cattcaataa ggtggattta cctacattgg tttttccgat gatagcgact ggaatcccat
      421 gtttaattac attgccatag cggaatgatt cgatgaggtt gtgaagtttt tgtcggattt
      481 cacccagtag ttccaacaaa tgctcgcgat tggcaaattc aacgtcttct tccgggaaat
      541 cgagctccaa ttcgataagt gaagtgaagt cgatgagttt ttggcgcatt tgctgtattt
      601 ccgacgaaaa tcctcctcgc atctgtcgga tagcgagttg atgagccata tgtgcttctg
      661 catttacaag gtcggctacg gcctcagcct gcgataaatc cattttccca ttcagaaaag
      721 cgcgaagggt aaattccccg ggtcttgcca ttaccgctcc atgcttcatt aataaagaca
      781 gcaaggtgtt ttgaatatac ctcgaaccat gacaggatat ttcaactaca tcttgtcctg
      841 tatacgaatg tggagcacga aatacggtta taaggccttc gtctatgatt tcatcttcat
      901 tgtgaaaatt tcctaaatga accgtgtggc tctttatatc ctttattttc agactcgatt
      961 tagccggttg aaaaattttt tcgatgatac gaaatgtatt aggacccgag agacgaataa
     1021 tggcaatagc tcccgtgccc tgaggcgtag ccggtgcaca aatggtagat tccatcgcaa
     1081 acatctccga taaggtattc agattggatt ccatgatttc ttgttttttt ttgattaaac
     1141 ttttccagat gctaaattgg ttcttttttc tgtaatggta caaattccta tttttctgaa
     1201 taagtcattt tatgcattct tctcttttgg attaatttta tttgcaagtc gacccacata
     1261 tgtcaatgct cgaaatgcat tgaccgtact cacctttcct cctttttacc tcttctcccc
     1321 gtcccctcgt ctccgttctc ccttcctccc ttataaactt ttaactttca acttttaact
     1381 catctatcca ccaaccaccg tttttctggt tcacttattt ttctatcctg atgcgggcat
     1441 caacggttgt catacttttc gaatttccaa ggagagggtt gaggtccatt tcgcgtattt
     1501 cgggagcagc ttgtaataat gacgaaactc ttaacaaaat atcggtaaaa attccttcat
     1561 tgattccttc tttccccctt actcccttga aaaggctata acttttcagg ttatggatca
     1621 tgctgacggc ttcgtcgtgg ctcaaaggcg ccaaggcagt cgaaaaatct ttcagggttt
     1681 ctattagtat gcctccaatt ccacaaagta taagatgacc aaatttgagt tcccagttag
     1741 cacctataaa aagctcggta cctgagagca taggctgaaa gagcactccc acggcatctt
     1801 caatcgccat gagttttgca aagttttgtt tgacctgttc gaggtcggat atattcagcg
     1861 caacg
//
LOCUS       DAORPD010000342         1856 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_11753_length_1856_cov_2.507496, whole genome shotgun sequence.
ACCESSION   DAORPD010000342 DAORPD010000000
VERSION     DAORPD010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11753_length_1856_cov_2.507496"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            253..>1856
                     /locus_tag="PK976_04755"
     CDS             253..>1856
                     /locus_tag="PK976_04755"
                     /inference="COORDINATES: protein motif:HMM:NF014896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRC67230.1"
                     /translation="MKTFHYPFTTILFTFIFMTHLQLDAQQNLRPLKQYLYPAPPIAA
                     SRPVEFREFGNTRIDPYFWLKDRNNPEVIAYLNAENEYCKLVMQETEPLQEEIFNEIK
                     SRIKEEDMSVKIPDNGYYYFTRTLKGKQYPLYYRQKMTPQAEEELIFDVNAMAESQPA
                     FIFSDYEVSPDNNFAAYSYNTTGSFAEFTLKIRDLRSGEDLPEQIENVQSFTWAADSK
                     TLFYTIGNESLRPYKVLRHIAGTLAEDTPVFEEDDELLNVSVSKTLSNDYILIESASF
                     TTSEVRMLESDQPDGNFQIFLPRKKDVMYGVEHHKNLGFYVYYKSPESKNGMVYLTPF
                     GKNSNPKQWKVIFPHDEKVKIENYEVFENHLVIQTRSNGLTGLRIYDLDGSKFHNVAF
                     PEPVYVVSTYTNPEYSQKVFRYSYSSLNRPTTIYQIDMDSHSVQTLKVQEIPSGFDPD
                     KYAVERRFAKATDGTDVPMAILYRKGLELNGSNPALLYGYGAYGYSTDAWFRTSVFSL
                     VDRGFVYAIAQVRGGSEMGEQWYEDG"
BASE COUNT          584 a          367 c          373 g          532 t
ORIGIN      
        1 ttgccagcgt catcgaaaaa ataaatgcca gcttgcatgg attattttga taagcaataa
       61 aattataaga aaataatcgg aaagtgtaac tttttggcat ccttgaaagt cgaagattca
      121 tctctgcaaa ctttattata gattatagat tttcgtttct ttgcaacaaa atattttgaa
      181 agcgaacgac gccttatact tttagtgaaa aactcactgc aacaatgcat cacatataat
      241 cttcaattca tcatgaaaac atttcattat ccattcacca ctattttatt tacattcatc
      301 tttatgacac acttacaact cgacgctcag caaaatttgc gtcctttgaa gcaatatctt
      361 tatcctgcac cgcctatagc cgcttcgcgc cctgtagaat ttcgtgagtt tggaaataca
      421 cgaatcgatc cttatttctg gctgaaagac cgcaataatc ccgaggtgat tgcttacctg
      481 aacgccgaaa atgaatattg caaactggtt atgcaagaaa ctgaacctct ccaggaagaa
      541 atcttcaatg aaataaaatc gcgtatcaaa gaagaagata tgagcgtaaa aattcctgac
      601 aacggttatt attactttac gcgtacgctt aaaggaaagc aatatccctt gtattatcgt
      661 caaaaaatga ctccacaagc agaagaagag ctaattttcg acgtaaatgc catggccgag
      721 agccagccag cttttatttt tagtgattat gaagtgagcc ctgacaataa ttttgccgct
      781 tattcctata acacaaccgg ttcgtttgcc gaatttaccc ttaagataag agatctaaga
      841 agtggagaag atttacccga gcagatcgaa aatgttcagt cctttacttg ggcagccgat
      901 agtaaaacat tattctatac cattggaaat gaatccctcc gcccatataa ggttttacgc
      961 catattgcag gaactctcgc cgaagatact cctgttttcg aggaagatga cgaattgctg
     1021 aatgtttccg tatccaaaac cttgtcaaac gattatatat tgattgaatc ggctagcttt
     1081 acaacttctg aggtaagaat gctcgaatcc gatcaacccg acgggaattt ccagattttt
     1141 ttgccacgaa aaaaggatgt catgtatggt gtggaacatc ataaaaattt agggttctat
     1201 gtctattaca aatctcccga aagtaaaaat ggaatggttt atctaacacc tttcggcaaa
     1261 aactccaatc cgaaacaatg gaaagttatc tttcctcacg atgaaaaggt aaagatagag
     1321 aattatgagg tcttcgagaa tcatctggta atacagacac gcagcaatgg attgacggga
     1381 ttgagaattt acgatctgga cggaagcaag tttcataatg tggctttccc cgaaccggtt
     1441 tatgtggttt ccacttatac gaatcctgag tacagccaga aggtattcag gtattcctat
     1501 tcatcgctca atcgaccgac caccatatac caaatcgata tggatagcca tagcgtgcaa
     1561 acgctcaagg ttcaggaaat tccttcaggt tttgatcctg ataagtatgc agtggaacgt
     1621 agatttgcca aagctactga cggtacagat gtgcccatgg ccattttata caggaaggga
     1681 ctcgaattga atgggtcgaa tcctgccttg ttgtatggtt atggagctta tggttacagc
     1741 accgatgcat ggttccgtac ctcagttttc agtctggtgg ataggggatt tgtgtatgcg
     1801 attgctcagg tgcgtggagg aagtgagatg ggcgaacaat ggtacgaaga tggaaa
//
LOCUS       DAORPD010000343         1856 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_11755_length_1856_cov_1.837868, whole genome shotgun sequence.
ACCESSION   DAORPD010000343 DAORPD010000000
VERSION     DAORPD010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11755_length_1856_cov_1.837868"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..1184)
                     /locus_tag="PK976_04760"
     CDS             complement(105..1184)
                     /locus_tag="PK976_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRC67231.1"
                     /translation="MKSLKFTVCLFFALLAGQLYAQDASTEQKLEHLMKKVQDLEHEF
                     DILKKLSDDILWFDRVGDMAHIDKVYITGPPPQVVKNPDATGARNPLKFYCYVFIPRQ
                     LDYSKKYPLIILPHGGVHADFTTYHTHIIREMIYQGYIVIAPEYRGSTGYGKSFYEKI
                     DYGGLEIEDVHAARNYMVENYPFVDKNRIGLVGWSHGGLIALMDVFEHPSDYAVIFAG
                     VPVSDLIARMGYQGPSYQELFSADYHIGQTAAENTAEYRRRSPVWNAEKLQTPLLIHT
                     NTSDDDVNVLEVEHLIKSLKAANKKFEYEIFQEVEGGHSFDRIDTKTAKEIRVKIYRF
                     IGTYLKPPRQIQTVDQINKAAYFFE"
     gene            1556..>1856
                     /locus_tag="PK976_04765"
     CDS             1556..>1856
                     /locus_tag="PK976_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67232.1"
                     /translation="MDNSANLIGKNLEELRNIFMAAGYETKDPSLLAHNLYKKYGKPL
                     SSIEAYPKSLRWFLEDNFSIEVLQPILKLQSSDGTIKGLFESEGGKAFEAAWIPAA"
BASE COUNT          553 a          387 c          357 g          559 t
ORIGIN      
        1 catccggaac cacaatccga cgctctaacc gctgaactac gcccaccgta taattggatg
       61 caaaattaag aaaattttct gatttcaaaa cttcatgaaa gaaattattc gaagaaataa
      121 gctgctttgt tgatttgatc gacagtttgg atttgtcggg ggggttttaa ataagtccct
      181 ataaaccgat atatctttac tctgatttct tttgcggttt ttgtatctat tctgtcgaat
      241 gaatgtcctc cttccacttc ctgaaagatt tcatattcga atttcttgtt tgcggctttt
      301 aatgatttta tcaggtgttc tacctctaaa acattcacat cgtcgtcgct ggtgttggta
      361 tgaataagta atggcgtttg gagtttttct gcattccata ccggcgacct gcgacggtat
      421 tctgctgtat tctcggctgc ggtttggcca atatgataat cggccgaaaa gagttcctga
      481 taggatggcc cttgatatcc cattctggct atcaaatcac ttaccggcac acccgcaaaa
      541 atcacagcat aatccgatgg atgctcgaaa acatccatca aggctataag ccctccatga
      601 ctccatccca ccaatcctat cctgttcttg tcgacaaaag gataattttc gaccatatag
      661 tttcgtgctg cgtgaacatc ttcaatctcg aggccaccat aatcaatctt ttcgtagaat
      721 gattttccat agcctgttga accacgatat tcgggtgcaa ttacgatata tccttgataa
      781 atcatctccc gaataatatg ggtatgataa gtggtaaaat cggcatgaac acctccatgc
      841 ggcaatataa tcagtggata tttcttcgaa tagtcgagct gtcggggaat gaaaacataa
      901 caataaaatt tcaaaggatt tcgcgcacca gtggcatcag gattctttac tacctggggt
      961 ggtggccccg tgatatatac cttatcgata tgtgccatat cccctacccg atcgaaccat
     1021 aaaatatcgt cgctgagttt tttcaagata tcgaactcat gttccagatc ctgaaccttc
     1081 ttcataagat gctcaagctt ctgttctgtg gatgcatcct gagcatacaa ttgccctgcg
     1141 agcaaagcaa aaaacaagca cactgtgaat tttaagcttt tcataaccca aaaattttaa
     1201 acgaggtaaa tataagtgat ttataaatat cacactcgac aaaaggaaaa cgttcgaaaa
     1261 cataagctat ggattattta cgcatacttt ttgcaaattg tcaggaaaac cgttccggat
     1321 caccttgaat gtctcagtgg taaaaacctc caaaatttca ataatattcc ttcgaaattc
     1381 agacctaaca ggtcttagag gcctattggg tttaaaaaaa tttagtaatt ggagcttcca
     1441 catgtcgtaa attttgggag gtgagggctg aatgcgtcga tcgactaaac taagcctatg
     1501 tccttaccag ccattttatt gaaacatagg tatctttgcg gctgagcttt atgacatgga
     1561 taattcggca aatctcatag gtaaaaatct cgaagaacta agaaatattt ttatggcagc
     1621 cggctatgaa accaaagatc catcgctgct tgcgcacaat ttgtataaga aatatggcaa
     1681 acctttgagt tccattgagg cttatcctaa gtcattgagg tggtttctcg aagataattt
     1741 ttcaatagag gttttacaac ctattttaaa acttcaatcg tccgatggta cgataaaagg
     1801 tctattcgag agtgagggag ggaaagcgtt cgaagcagca tggattccag ccgcaa
//
LOCUS       DAORPD010000344         1852 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_11807_length_1852_cov_1.767390, whole genome shotgun sequence.
ACCESSION   DAORPD010000344 DAORPD010000000
VERSION     DAORPD010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11807_length_1852_cov_1.767390"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="PK976_04770"
     CDS             <1..428
                     /locus_tag="PK976_04770"
                     /inference="COORDINATES: protein motif:HMM:NF013315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HRC67233.1"
                     /translation="LLNVGPDDVVLEIGTGSGYQAAILSRIVKKVYTVEIVEELGLAA
                     KERLKALNYDNVEVKIGDGYYGWEEHAPYSGIIVTAASEYIPPPLIKQLKDKGRMIIP
                     VGSPFSVQNLMLVEKSGDKIRTKSLIPVRFVPLTRQSAP"
     gene            477..>1852
                     /locus_tag="PK976_04775"
     CDS             477..>1852
                     /locus_tag="PK976_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67234.1"
                     /translation="MESNSQLSLIRINVAIGLISAAIVAFQLILMQILSYVQWYHFAY
                     MIISIALLGFGAAGTLLTIFRKKLEQNYEAIFPFLLIITAILIPLVVGMANSNAIRFD
                     SLLIFQDSRHIGRLILTYFIFFLPFLTGALAIGLSFSKFADRIGKIYFSNLIGSGIGG
                     IIALGFMQWIIPEQQPFFVALPAFLAGMVSIPKNAKPFLRITLPVSAIILLILFFYPP
                     KLIPSEYKDISKTMLLPEATVDYKKSSPHGLVEIVSSPVLRFAPGVSLTYQEPFPVRK
                     TVFNNGNWAGSLLPQPLKQNQTSILDYTPQALPYHIGKIKNALILDAGTGENVLLASS
                     HHVTHITANEANAEIFRLLQDSFEDFSQVQLHQTMSRTLLIPDTMRYDLIELPVIGSF
                     FGNSGLNAVEPRYELTLEALQEMWHKLSEKGMISLSCWMDYPVRNAYRLLSTISLLLE
                     KNELENYQ"
BASE COUNT          515 a          412 c          383 g          542 t
ORIGIN      
        1 aattactaaa tgtaggaccc gatgatgtgg tattggaaat aggaacaggt tcaggttatc
       61 aggctgccat tttatcgcgg atcgtaaaaa aagtatatac tgttgaaatt gttgaagagc
      121 ttggcctcgc cgcaaaagag cggttaaaag cgctgaatta cgataacgtg gaagtaaaaa
      181 taggcgatgg ttactatgga tgggaagaac atgcgcccta cagcggcatc attgttaccg
      241 cagcgtcgga atatattcca ccgccattga ttaagcaatt gaaagataaa gggcgtatga
      301 ttattccggt gggctctcct ttcagcgttc aaaaccttat gttggttgag aaatcaggag
      361 acaaaataag gactaaaagt ctgattcctg taagatttgt acctttaacc cgacaaagtg
      421 caccctaacc gcagcaaccg attaagaaaa aatcgttgaa aacaggcaaa taaaaaatgg
      481 aatcgaacag ccaactatcc ctgattcgca tcaatgtcgc aatcggattg atttcagcgg
      541 ccatcgttgc ttttcaattg atattgatgc agattttgtc gtacgttcaa tggtatcact
      601 ttgcctacat gatcatatcg atagccctgc tcggctttgg tgctgccgga acgttgctga
      661 caattttccg aaaaaaactg gaacaaaatt atgaggcgat tttccctttt ttattaatta
      721 ttactgcgat tctcatccca ctcgtggttg ggatggccaa ttcgaatgct attcgtttcg
      781 attccttact catctttcag gattctcgcc acattggccg attaatcctt acctatttta
      841 tcttttttct gccttttctg acaggcgcat tggccattgg actaagtttt tcgaaatttg
      901 ccgaccggat tggaaaaatc tatttttcca atctcatcgg atcgggtatc gggggaatca
      961 ttgccttagg tttcatgcaa tggataattc ccgaacaaca accctttttc gtagctttgc
     1021 cggccttttt agccggaatg gtctcaattc caaaaaatgc aaagccattt cttcggatta
     1081 ctctgcctgt ttcggcaatc atattgttga ttctattctt ttatccgccc aaacttatcc
     1141 cgtccgaata taaagatatc agcaaaacca tgctgttgcc cgaggccacg gtcgattaca
     1201 agaagtcctc tcctcacgga cttgtagaga tcgtttcatc gccggtactg cgttttgcac
     1261 ccggggtaag tcttacttat caggagcctt tccctgtacg aaaaaccgtt tttaataacg
     1321 gaaactgggc tgggagtctt ctaccgcaac ccttaaagca aaaccaaacc agtatccttg
     1381 actatacccc gcaagctttg ccttatcata tcggaaaaat taaaaacgct ttgatccttg
     1441 atgctggaac aggggaaaat gtgttgctgg catcatcgca tcatgtgacc catattaccg
     1501 ctaacgaagc caatgcagaa atctttcgat tgctccaaga ctctttcgaa gacttttcgc
     1561 aagttcagct ccatcaaacc atgtcgcgaa ctttacttat tcccgacacg atgaggtacg
     1621 atttgataga acttccggtt atcggttcgt ttttcggcaa ttcgggtctg aatgccgttg
     1681 agccccgata cgaattaact ctggaagctt tacaagaaat gtggcacaag ctttcggaaa
     1741 aaggaatgat ctcattgagc tgctggatgg attatccggt gagaaacgct tatcggcttt
     1801 tatccacgat tagtcttctt ctcgaaaaaa atgaactcga gaattaccag ca
//
LOCUS       DAORPD010000345         1851 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_11817_length_1851_cov_2.400891, whole genome shotgun sequence.
ACCESSION   DAORPD010000345 DAORPD010000000
VERSION     DAORPD010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11817_length_1851_cov_2.400891"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /locus_tag="PK976_04780"
     CDS             <1..891
                     /locus_tag="PK976_04780"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HRC67235.1"
                     /translation="WDFGMNSLFKGGNINAWAQFKNYWQISAGLNFDGNNLDNYVLRG
                     GPALKLPGGYSYWGYLSTDSRKKLVAEINASIYRTFIANNNYNFFGVGMHYQPFTALS
                     FGIFPEYSVNNDNLQYVKEKKMNGVPRYIMAYIHQKTLALEMRINLNLSNDLTLQYYG
                     QPFISSGEYSHFKFISSPKANDYYQRFTEYSEAQIFYQPSDKTYQIDEDYDGIPDYSF
                     NKPDFKALYFVSNLVLRWEYRPGSFIYVVWSQNRADQGKEISFKPKKDITSLFDIYPG
                     NTFMVKISYLIQYQHLKRIF"
     gene            complement(874..1257)
                     /locus_tag="PK976_04785"
     CDS             complement(874..1257)
                     /locus_tag="PK976_04785"
                     /inference="COORDINATES: protein motif:HMM:NF013633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HRC67236.1"
                     /translation="MSEEEIRIDKWLWAVRVFKTRSLASQACRSGKVRINGMVVKPSR
                     DIRCGDVIQISLGSLNKTVEVKAILHNRISAKEVSTYLIDRTPVEEYERQMIIAEMKQ
                     EIRPKGKGRPTKRERRDIDRLKDSF"
     gene            complement(1254..>1851)
                     /locus_tag="PK976_04790"
     CDS             complement(1254..>1851)
                     /locus_tag="PK976_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRC67237.1"
                     /translation="KVEENIEFYSVMRSVRAIENSDVCILMADANVGFQQQDMNIFSL
                     IQRNHKGMVILMNKWDLVNKDSKTLRIYEEEVHRKIEPFTDVPVLFTSVHEKKRLLKA
                     LEWAIKVYENRKQRITTSRLNEVMLPIIGQNPPPVVKGKVVKIKYATQLPKPYPVFLF
                     FCNHPQYIREDYKRFIENKLRENFSFTGVPIEIYFREK"
BASE COUNT          543 a          378 c          326 g          604 t
ORIGIN      
        1 tgggattttg gaatgaacag cctttttaag ggaggaaata tcaacgcatg ggcccaattc
       61 aaaaactatt ggcaaatcag cgcaggattg aatttcgatg gcaataacct cgataattac
      121 gttctaagag gtggacctgc tttaaaattg cctggcggat attcttattg gggttattta
      181 tcaaccgact cacggaaaaa gctcgtagca gaaatcaatg cgagcattta tcgaacattt
      241 atcgccaata ataactataa tttttttgga gttgggatgc attatcaacc ttttacggct
      301 ttgagcttcg gtatttttcc agaatattcg gtaaataacg acaacttgca gtacgtgaag
      361 gaaaagaaaa tgaatggagt accccgctac atcatggctt acattcatca aaaaacctta
      421 gcccttgaaa tgcgtattaa cttgaatctc tccaacgatc ttacacttca atattatggc
      481 caacccttta tttcgagcgg agaatattcg cattttaaat ttatctcttc tccaaaggct
      541 aatgattatt accagcgatt tacggaatat tccgaagctc agatttttta tcaaccatca
      601 gacaaaactt atcaaattga tgaagattat gatggaatac cagattattc atttaacaag
      661 cctgatttca aagccttgta ttttgtatcg aatctggtat tgagatggga atatcgtcca
      721 ggttcgttta tttatgttgt ttggtcgcaa aaccgtgccg atcaagggaa agaaataagt
      781 ttcaagccaa aaaaggatat tacatcactc ttcgacattt atccgggaaa tacttttatg
      841 gtaaaaataa gttatttaat tcaatatcag cacttaaaaa gaatctttta agcggtcgat
      901 atctcgcctt tccctttttg ttggccgccc tttgccttta ggcctaattt cttgcttcat
      961 ttcagcaatt atcatttgtc gttcatattc ttcaacaggt gtacggtcga tcaagtaagt
     1021 cgatacttcc ttcgccgaaa tacgattatg cagaattgcc tttacttcga ctgttttatt
     1081 caagctaccc aaactaattt gaatgacatc tccgcagcga atatcccgtg aaggtttcac
     1141 aaccatacca tttattctga ccttgcctga gcgacaagcc tggcttgcca gtgaacgtgt
     1201 cttaaacaca cgtactgccc atagccattt atcgatacga atttcttcct cactcatttt
     1261 tctctgaaat aaatctcgat tggaacgcca gtaaaagaaa aattctcacg taatttattc
     1321 tcgatgaatc tcttgtaatc ttctcgaatg tactggggat gattgcagaa gaaaaggaaa
     1381 accggataag gtttcggcaa ttgtgtagca tattttattt tcaccacttt tcctttcact
     1441 actggaggag gattctgacc gataataggt agcatcactt cattgagacg ggaggtagta
     1501 atgcgttgct ttcggttttc ataaacttta atagcccatt ccaatgcctt gagcaaacgt
     1561 ttcttttcgt gtaccgacgt aaataaaaca ggaacatcag taaagggttc gatttttcta
     1621 tgaacttctt cctcgtaaat tctcagggtt ttgctgtctt tattcaccaa atcccactta
     1681 ttcataagga tcaccattcc tttatgattt ctctgtataa ggctgaagat gttcatgtct
     1741 tgctgttgga atccaacatt tgcatcagcc attaaaatac agacatcgct gttttcgata
     1801 gccctcactg atcgcatcac cgaatagaat tcgatattct cttccacttt t
//
LOCUS       DAORPD010000346         1850 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_11830_length_1850_cov_2.210585, whole genome shotgun sequence.
ACCESSION   DAORPD010000346 DAORPD010000000
VERSION     DAORPD010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11830_length_1850_cov_2.210585"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1686)
                     /locus_tag="PK976_04795"
     CDS             complement(<1..1686)
                     /locus_tag="PK976_04795"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRC67238.1"
                     /translation="MKRFLLSFILMVYMTSISTFSQIAPEKYVAWLSDKANNPYSLSN
                     PSEFLSERALERRARYNIPLDNKDLPVTPQYVERIAQIGVQVLHSSKWLNFVMFQTAD
                     HSLVEQIQQLEFVIKVEKMKRRIRTEPCPVNKVLYESYYPKPNEINFFSSQLNLKFSS
                     LTANLDYGAAYTQIHMVKGDELHRMGYLGDGMLIVLLDAGFNSADINPAFDSLWMKGR
                     ILGYRDIVQRGNNVFSTSMSPHGAYVLSIMGANLPGQMVGTAPHASYYLIRTEDDGSE
                     YIIEEYNWVTGAELADSLGADIINSSLGYTVFDDPSQNHTYADMDGNTAISSRGADIA
                     ASKGILVVNSAGNSGSSAWHYIGAPADGDSVFTIGAVDRWGYYASFSSTGPTYDGRIK
                     PNVMAMGQGTAVVDIYGNVTYGSGTSFSSPLTAGCIACLWQSKLGKNNMTVMQSVMQT
                     ASQFNKPDSLMGYGIPDFLAAFQTLSVGQRECANHNNFTVSPSFFIDNLIITNELNRD
                     ENFSLSLLDVQGKLILEKEFQGGNRYFYLRDEIRNLKPGLYILQISYSSGFDSF"
BASE COUNT          570 a          390 c          377 g          513 t
ORIGIN      
        1 aaaagagtca aaacctgacg aataggaaat ctgaaggata taaagacctg gttttaaatt
       61 tcttatttcg tccctcagat agaaatatct gttaccaccc tggaattctt tttcaaggat
      121 gagtttacct tgaacatcaa gtaatgaaag ggaaaaattt tcatcccgat tcaattcatt
      181 tgtaataatc aaattgtcaa tgaaaaaaga tggagaaact gtaaagttgt tatgattggc
      241 acattctctt tgtcctacag aaagggtttg gaaagcagct aaaaaatcag gtatgccata
      301 acccattaaa gaatcgggtt tattgaattg tgaagcggtt tgcattaccg attgcattac
      361 cgtcatatta tttttcccga gtttagattg ccataaacat gcgatgcatc cagcagtcaa
      421 tggagaagag aaggaagttc cactgccgta tgtcacatta ccgtatatat caaccactgc
      481 agtgccctgt cccatagcca tcacattggg tttgatacgg ccatcgtaag ttgggccggt
      541 ggaactgaat gaagcataat atccccagcg atccacggct cctatggtaa aaacactatc
      601 accatcggcc ggggcaccaa tgtaatgcca tgccgaactg ccactattgc cagcactgtt
      661 gactaccaga atccctttgc tggcggcaat atcggctcct cggctcgaaa tggcagtatt
      721 tccgtccata tcggcatagg tatggttttg agaaggatcg tcgaagacgg tgtatcccaa
      781 ggaagagttg atgatatcgg cgcccaggct gtcggctaat tcagcaccgg taacccaatt
      841 gtattcttcg attatatatt ccgagccatc gtcttcggtt cgaataagat aataggaagc
      901 atgcggggcc gttccaacca tctgcccggg aagattggcc cccatgatag ataatacata
      961 tgcaccatga gggctcatcg aggtagaaaa aacattattg cctcgttgca ctatatcacg
     1021 gtaacccaaa attctcccct tcatccaaag gctgtcgaag gcagggttta tatctgccga
     1081 attaaaacct gcatccagaa ggacaatcaa cattccgtcc ccaagatatc ccattcgatg
     1141 cagttcatcg ccctttacca tgtgaatctg tgtataagcc gctccataat caaggtttgc
     1201 tgtcaaagaa gaaaatttta gattcaattg tgaagagaaa aaatttattt cattgggttt
     1261 aggataataa ctctcataca aaactttgtt tacgggacaa ggctctgttc gaattcgccg
     1321 cttcatcttc tccaccttaa taacaaactc aagctgctga atctgttcga cgagtgaatg
     1381 atcagcagtt tgaaacataa cgaaattcag ccatttggat gaatgcagaa cttgaactcc
     1441 aatttgagca attcgctcga cgtattgtgg agttacgggc aaatccttat tatcaagagg
     1501 aatattgtaa cgcgccctgc gctccaaagc tcgctcagaa agaaattccg atggattaga
     1561 cagactgtat ggattatttg ctttgtcaga taaccaggct acatattttt caggagcaat
     1621 ttgtgaaaaa gtgctgattg aagtcatata aaccattaaa ataaaactga gtagaaatct
     1681 cttcatggta aaaaagatga tgttatttag aatgatgatg tcgctaaaat tctattattt
     1741 attttaaaga aagctaatct ctatgagaga gctcaagaag caaaattacg gctttttggc
     1801 taaaatcgag taggtaggga gattgctccc ctgccctctc accccaccca
//
LOCUS       DAORPD010000347         1849 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_11839_length_1849_cov_1.507246, whole genome shotgun sequence.
ACCESSION   DAORPD010000347 DAORPD010000000
VERSION     DAORPD010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11839_length_1849_cov_1.507246"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1849
                     /locus_tag="PK976_04800"
     CDS             <1..>1849
                     /locus_tag="PK976_04800"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HRC67239.1"
                     /translation="TDYLFIYNDEINANEKVSVKAKQEAVSSVLNSMLEDKDIKYSME
                     GNHIILSKSEPSENENITNVLQQQKKRISGTVLDENGQPIIGANIIEAGTTNGTVTDI
                     DGKFTLDVSDNATIRISYIGYLEQEVNTSGKNTLNITLVEDTQTLEEVVVVGYGIEKK
                     VNIIGSIATVGTDDLNASPVSNISNAIAGRLPGVVIQQATGEPGNDAATIRIRGRATL
                     GDSEPLVVVDGIPGRDLNSINVYDVENISVLKDASAAIYGARAANGVILITTKQGKED
                     APLTVNYGFYQGFSSPTVKIKMADAPTYAQMIREVQTYQNVDEANMKFSLDDIEKFKS
                     GEYPWTHPNTDWYDAALKNYSTTWNHNVSINGGSKAADYYISFGTHYDGGLFKNSSTS
                     FTRNNIKATVNAEINKYLSLGLDINGSQENKKNPSVSAWGNFEGIIKSLPTSPAFFPN
                     GLPGPDIAYGGNPVVTSTDQTGFDNSKRYRANTMFSANLKIPGIEGLVLSSYYAYDLN
                     IGQRKLFQKPWMLYELDEPAYLAAGNTGKEDGSAFLIGTEKGTTDIWLRNYYDDAKTK
                     TFNVKADYKTTINDVHNISAFIAYESSEYESKGIDAYRRNLISDQLPYL"
BASE COUNT          637 a          320 c          389 g          503 t
ORIGIN      
        1 aaaccgatta tctgttcatt tacaacgatg aaataaatgc caacgaaaaa gtatcggtga
       61 aagctaagca agaagcagtt tcgagtgtac taaattcgat gctcgaagat aaagacataa
      121 aatattcgat ggaaggaaat catattatcc tttctaaaag tgaaccatcg gaaaacgaga
      181 acataacgaa tgtccttcaa caacagaaaa aacgaatctc gggaacagtg cttgacgaaa
      241 atgggcaacc cattattggc gccaatatca ttgaagccgg cactaccaac ggaacagtaa
      301 ccgacataga tggcaaattc actttagatg tttcagataa tgccactatt cgaatttcct
      361 atatcggata tctcgaacaa gaagtgaata catccggcaa aaatacccta aacattaccc
      421 tagtagagga tacacaaacg ttagaggaag tggttgttgt ggggtatggt atagaaaaaa
      481 aggtaaacat aataggatca atagcgactg ttggaacgga tgacctcaat gcctcccctg
      541 tttcaaatat ttcaaatgcc atcgctggcc gccttccagg ggtcgtaatt caacaagcaa
      601 caggtgagcc tggtaacgac gcagcaacca ttcggatcag gggtagggct actttgggcg
      661 acagcgagcc tttggtagtt gttgatggta ttcctggaag ggatttgaat tcaattaatg
      721 tttacgatgt tgaaaatatc agtgttctta aagatgcgtc agcagcgatt tatggtgcca
      781 gggctgccaa tggtgtgatt cttattacaa ccaaacaagg caaggaggat gcaccattaa
      841 cagttaatta cggattttat caaggttttt cttccccaac agtaaaaatc aaaatggcag
      901 atgcaccaac gtatgcgcaa atgattcgtg aagtacaaac atatcaaaat gtagacgaag
      961 ctaatatgaa gttttcattg gatgatattg aaaaatttaa atccggagaa tatccatgga
     1021 ctcatccaaa tacagattgg tatgatgctg cattaaaaaa ttacagcacg acatggaatc
     1081 ataacgtgtc gataaatgga gggagtaaag ccgcagatta ctatatatcc tttggtacgc
     1141 attatgacgg aggattattt aaaaattcat ccacttcatt tacgagaaat aatattaaag
     1201 ctactgttaa tgcggaaatt aacaaatatc tgagtcttgg acttgatata aatggctctc
     1261 aggaaaataa aaagaatccc tctgtaagtg catggggtaa ttttgaggga ataattaaga
     1321 gtcttcctac ctcacctgca tttttcccaa atggattacc cggacctgat attgcttacg
     1381 ggggaaatcc tgttgtaacc tcaactgatc aaacggggtt tgacaattca aaacgctaca
     1441 gggctaatac gatgtttagt gctaacttga aaattccagg tatcgagggg ttagtcttgt
     1501 cgagttatta tgcttatgat ttgaacatag gtcagagaaa actatttcaa aaaccatgga
     1561 tgctttatga attggatgaa ccagcctatc tggcggcagg aaatacagga aaggaggatg
     1621 gatctgcatt cttgattggt actgagaagg gaactactga catctggtta agaaattatt
     1681 atgatgatgc caaaactaaa acttttaatg tcaaagctga ttataaaaca accattaacg
     1741 atgttcataa tataagtgca tttattgcat acgaaagttc tgaatatgag tctaaaggga
     1801 ttgatgcata ccgtaggaat ttaattagtg atcaattgcc ttacttatt
//
LOCUS       DAORPD010000348         1848 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_11847_length_1848_cov_2.220859, whole genome shotgun sequence.
ACCESSION   DAORPD010000348 DAORPD010000000
VERSION     DAORPD010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11847_length_1848_cov_2.220859"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="PK976_04805"
     CDS             <1..285
                     /locus_tag="PK976_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67240.1"
                     /translation="RLHADLSFHHDGLILAGLYDWGFPIAPGFIFYPGVGGIVYIGDV
                     FRLGVTGELGFEYAFDIPLSIGLDWRPIITIIDEGGFSGDGFGLNIRYRF"
     gene            763..1728
                     /locus_tag="PK976_04810"
     CDS             763..1728
                     /locus_tag="PK976_04810"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67241.1"
                     /translation="MKKMTFILGLAIASIIFLAPQKKICAQTLEDAGNTFNSAIQLMQ
                     NDLPSAISKFEEVIGMCEKIGESGNELKMQAQNQLPILYYNYAVNLQASQNYDQAIEA
                     CNKAIQTGQQVNNADIIAKAVNLTAQIYMVKGNEYFKNKDFANALVSFDQAITLDSTL
                     YRAYYGKLLVYREQDKYDEMDEMLEKVKTTSTQDPQFAEKAQALVAREYSIAAAKAIQ
                     AKNSSLALTYCDKALALGDATANTYYYQTLAANMEKKYSLAAEAAQKALELEQKDKSK
                     IYFELGKAYEGLGRAADACAAYKNVTDGPLVQNAQYQIKQVLKCK"
BASE COUNT          589 a          386 c          349 g          524 t
ORIGIN      
        1 cgtcttcatg ccgatctgtc ttttcatcac gatggcctaa ttttggccgg attatacgat
       61 tggggcttcc ctattgcacc tggttttatt ttctatcccg gtgtgggagg catcgtatat
      121 attggagatg tatttcgcct tggagttaca ggagaattgg gattcgagta tgctttcgat
      181 attcctttga gtattggatt ggactggcgt ccaataataa ctatcataga tgaaggaggt
      241 ttctcaggag acggttttgg tttgaacatt cgataccgtt tttgattcgt aacataaggc
      301 catgaaacat gatgtttttg agtttgtcga gaaaataatc catgcggtta gttataactg
      361 attttagctg gaaagctata taaacaaaag gaaaacaccc caattccaac taaatttaaa
      421 actcaaaatt ctaacatctc tatcccataa tcttttcaac tttatagcca tagtcgaaag
      481 aaccttattt tttttcgctg atttacgctg ctttaatacg ctgatattaa ctaataactg
      541 acaacgaaca acaaacaagc ccccccacaa tcaccatcca ccatccacct tgtctcccag
      601 tctcccttcg tcctttcctc ctttataaac ttttaacgtt ctacccccca ttctcgataa
      661 aaacgaattt ttatccgtca gtgtaccgat tgaaaattat catgtaaatt tgtggtctta
      721 aatgaaaggt aaacaacgat tattataatt aaaacattga agatgaaaaa gatgactttt
      781 attttagggt tagccattgc aagtattata ttcttggctc cccaaaagaa aatttgcgct
      841 caaaccctcg aagacgcagg aaacacattt aattctgcta ttcaactgat gcagaacgat
      901 ctgccaagcg ctatttcaaa gtttgaagaa gtgatcggca tgtgcgagaa aataggcgaa
      961 agtgggaatg aattgaaaat gcaggcccag aaccagctac ccattctgta ttacaattat
     1021 gcagtaaatt tacaagcttc tcaaaattat gatcaagcaa tagaagcatg taataaagcc
     1081 attcaaacgg gccagcaggt gaacaatgcc gatatcattg caaaagcggt caacttgact
     1141 gctcaaattt atatggtaaa gggtaacgaa tacttcaaga acaaagattt tgcgaatgcg
     1201 ctggtcagct ttgatcaagc tattacgctc gacagtaccc tataccgagc ttattatggc
     1261 aaattactgg tttataggga gcaagacaaa tatgatgaaa tggatgagat gctcgagaag
     1321 gtaaaaacca ccagcactca ggacccccag ttcgcagaaa aagctcaggc tctcgtggcc
     1381 agagagtatt ccattgccgc tgcaaaagcc attcaagcaa aaaatagcag ccttgctctt
     1441 acctattgtg acaaagccct tgcattgggc gatgctacgg ctaacactta ctattatcaa
     1501 accttagcag caaatatgga gaaaaaatac agcctggcag cagaagcagc tcaaaaggca
     1561 ctcgagctcg aacaaaaaga taagagcaag atttacttcg aattgggcaa ggcatacgaa
     1621 ggacttggta gggccgccga tgcctgtgca gcttataaaa atgttactga tggtccactg
     1681 gttcaaaatg ctcaatacca aataaaacag gtgctgaaat gtaaatagaa cattccacct
     1741 tttataatcc taaaactctt accaaattgc gggagattcg aaagagtctt ccgctttttc
     1801 tgtttttcag ttaacaacca gctctcattt ttctccggca aatttcct
//
LOCUS       DAORPD010000349         1846 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_11857_length_1846_cov_2.882189, whole genome shotgun sequence.
ACCESSION   DAORPD010000349 DAORPD010000000
VERSION     DAORPD010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11857_length_1846_cov_2.882189"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..781
                     /locus_tag="PK976_04815"
     CDS             <1..781
                     /locus_tag="PK976_04815"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HRC67242.1"
                     /translation="KALPIGVVLTLPATGSEMNSGAVISKESTKEKCSFFSPLVFPRF
                     SVTDPSFTFSLPPRQVANGIVDAFIHTMEQYLTYPVSAPLQDRMAESILNTLMEEGPK
                     AFTKPYDYDTRANIMWCATLALNGLIACGVPTDWATHGIGHELTALHGLDHALTLAIV
                     FPGVMKVMQDEKREKILQYGERVLKITQGTEKERIEQAILKTDEFFRSLGMKTRLSEN
                     GIDQDAIETIVERFSKPRMICLGENQSITPVKIRQILLDRL"
     gene            806..>1846
                     /locus_tag="PK976_04820"
     CDS             806..>1846
                     /locus_tag="PK976_04820"
                     /inference="COORDINATES: protein motif:HMM:NF014301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HRC67243.1"
                     /translation="MKLFKNLLFRSFLIILFILRIFSIVEAQEEGISPEIIKNHIDFL
                     ASDKLQGRKTPSAGLDTAASYIAHELQNLGINSLNGTYFHSLNLCRCGLGKRQHFELN
                     TPSGKVVVPLKTGYIPFQTIGDTTVEGVLVFAGYGLNLPNLGYDDYSNIDVKGKIVVV
                     LSHFPGETSDDHRAKLIEKSGKTSINEKIATARNKGAIGLILLTNPKNHLLLKPQGHT
                     WAALLKRAKEDSDPLILKFHKESFTAIHAGEETMKLLFGSKENLIKIQEKIDKNFQPY
                     SFEIPGFTASIATSLHKRFIKADNVIGILEGNNPKLRSQYLIIGAHYDHIGTMKSEGT
                     DNSDTIFNGADDN"
BASE COUNT          572 a          383 c          379 g          512 t
ORIGIN      
        1 gaaagctctt ccgattggtg tagtgcttac tttgccggca acgggaagtg agatgaattc
       61 aggagcggta atctcgaaag aaagcaccaa agaaaaatgt tctttcttca gtcctttggt
      121 tttcccacgt ttttcggtca ccgatccttc atttaccttt tccttgccgc cacggcaggt
      181 agccaatggt atcgttgatg cctttattca caccatggaa caatatctta cttatcctgt
      241 gagtgcaccc ttacaagacc gcatggcaga aagcatactg aatactttaa tggaggaagg
      301 tccaaaagct ttcacaaaac cctatgatta tgacacacgt gcaaatatca tgtggtgtgc
      361 taccttggcg ttgaatggat tgatcgcttg tggtgttccc accgattggg ctactcatgg
      421 catagggcat gaactcaccg ctctgcatgg tctcgaccat gccttgacgc ttgccattgt
      481 ctttcccggc gtgatgaagg tgatgcagga tgaaaaacgc gaaaaaattc ttcagtatgg
      541 tgaaagagtc ttaaaaatta ctcaaggaac cgaaaaggaa agaatagagc aggccatatt
      601 aaaaaccgat gaattcttcc gctctttagg gatgaaaacc cgcctgagtg aaaatggtat
      661 agaccaggat gccatcgaaa ccattgtcga acgcttttct aaacctcgaa tgatttgttt
      721 gggcgaaaac caaagcatta ctcccgtaaa aatccgtcag attttgttgg atagacttta
      781 atcaatgaac tccaatgtat aatttatgaa actctttaaa aatttattat ttaggtcgtt
      841 tctcatcatc ttgttcattc tgagaatatt ttccatagtt gaagcacagg aggaaggcat
      901 ttctcccgaa atcataaaga atcacataga ttttctggca tccgacaagc tgcaaggacg
      961 aaaaactcca agtgcaggtc tggatactgc tgcaagctat attgctcacg aattgcaaaa
     1021 tcttggaatt aattccctga acggcactta cttccattcc ttgaatttat gcagatgtgg
     1081 tctgggcaaa cgacagcact tcgaattgaa tacaccctca gggaaagttg tcgtcccgtt
     1141 gaaaacaggt tatattcctt ttcagactat aggcgatact accgttgaag gagtattggt
     1201 ctttgcaggt tatggactca acctgcccaa tttgggatat gacgattaca gcaatattga
     1261 tgtaaaaggt aagattgtgg tggtactaag ccattttcct ggtgaaactt ctgatgatca
     1321 cagagcaaaa ctgatcgaaa aatcagggaa aacatctatc aatgagaaaa ttgctactgc
     1381 acgaaacaaa ggagctatcg gcctgatttt acttacaaac cccaagaatc atctcttgtt
     1441 gaaacctcag gggcacactt gggcagccct tttgaaaaga gcaaaagaag attccgatcc
     1501 gctgattttg aaatttcaca aggaatcatt tactgccata catgctggtg aggaaacgat
     1561 gaaattattg tttggcagca aagaaaacct catcaagatt caagaaaaaa tcgacaagaa
     1621 ttttcaacct tattcttttg aaattcccgg atttacagca tccattgcaa caagtcttca
     1681 caaacgtttt ataaaagccg acaacgtcat tggaattctc gaaggcaaca acccaaaatt
     1741 gaggtcgcag tatctcatca ttggggctca ttacgatcat attggaacaa tgaaatccga
     1801 aggaactgat aattcggata ctattttcaa tggtgccgac gataat
//
LOCUS       DAORPD010000350         1846 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_11858_length_1846_cov_2.487996, whole genome shotgun sequence.
ACCESSION   DAORPD010000350 DAORPD010000000
VERSION     DAORPD010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11858_length_1846_cov_2.487996"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1336
                     /locus_tag="PK976_04825"
     CDS             <1..1336
                     /locus_tag="PK976_04825"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HRC67244.1"
                     /translation="KKVLILGAGLIARPIVKHLLEQGYLVTVATRTKSKADEMIKKHP
                     NGTAIAWTVENEDMLESMVHENDLTVSLLPYAYHVLVAKACIKAKKHMVTTSYVKPEM
                     QALDEQVKEAGIIILNEMGLDPGIDHMSAMRIIDHIHQKGGKIEQFYSLCGALPAPEA
                     ADNYFRYKFSWSPKGVVMAGNNDACFLMNGKIIEIPSKDLFKNSMKVCFPEVGMLEVY
                     PNRDSILYKEIYGIEEAKTMMRGTFRYPGWCVTIDTMKALNLISYDKYNFAGKTYAEF
                     VGMMIGIEDASGIRQKVADYLGIDVNNYALQAMQWLGLFDDKPMGRAIDSPFEITSDL
                     MISKMQLGDNEHDMVVMQHNFLAVYPDGKREVIRSRMLDYGTPTTDTSIARTVALPAA
                     LGVRMILEGKINIKGVYRPVLPEIYNPVLDGLEELGIKMNEEYGLPESEILL"
BASE COUNT          584 a          316 c          399 g          547 t
ORIGIN      
        1 gaagaaagta ttaattcttg gagccggctt aatagcaaga cctattgtaa agcatctgtt
       61 ggaacagggc tatttggtta cagtggctac acgtacaaag tcgaaagccg atgagatgat
      121 caagaagcat cctaatggca cggccatcgc atggactgtc gaaaatgaag atatgctcga
      181 gagcatggtt cacgaaaatg atcttaccgt aagcttatta ccctatgctt atcatgtatt
      241 ggttgccaaa gcctgtatca aggccaaaaa acacatggta accacctcct atgtcaaacc
      301 cgaaatgcaa gcgctcgatg aacaggtaaa agaagctggc attatcattc tcaacgagat
      361 gggcctcgat ccaggtatcg atcacatgtc ggcaatgaga attatcgatc atatccatca
      421 aaagggagga aaaatagaac aattttatag tctttgcgga gctttacctg cacctgaagc
      481 agccgataat tacttcagat ataaattctc atggagccct aaaggagtag ttatggcagg
      541 aaataatgat gcctgttttt tgatgaatgg aaaaattatc gaaattccat cgaaagatct
      601 gtttaagaat tcaatgaaag tatgtttccc cgaagttgga atgcttgagg tttatcccaa
      661 tcgcgactcg attttgtata aagaaattta tggaatcgaa gaggccaaga ctatgatgag
      721 gggaactttt agatatccag ggtggtgtgt aaccattgat accatgaaag cattgaattt
      781 gatttcgtac gataaataca atttcgctgg aaaaacttat gctgaatttg ttggaatgat
      841 gataggaatc gaagatgctt ctggcattcg tcaaaaggtg gccgattatc tgggaattga
      901 tgtaaataat tatgctttgc aagccatgca atggcttggc ctgttcgatg ataaacctat
      961 gggacgtgca atcgattctc cttttgaaat tacttccgat ctgatgattt cgaagatgca
     1021 attgggcgat aatgagcacg atatggtggt gatgcaacac aattttttgg cggtttatcc
     1081 tgatggaaaa cgcgaagtaa tccgctcgcg catgctcgat tatggaacac cgacaaccga
     1141 tacgagcatt gcccgcaccg tagccttacc tgctgcttta ggcgttagaa tgattttaga
     1201 agggaaaata aacattaaag gcgtttatcg acctgtatta ccagaaattt ataatccggt
     1261 tctcgatgga ttagaagaac ttggaatcaa gatgaatgaa gaatatggcc tcccggaaag
     1321 tgagattctt ctttaaaacc aatcgatgaa atttgagttt tgggctggca agactttttt
     1381 accagcccaa aatgtattca tactttttta agtttctctg aaagctcgca ttatcatctt
     1441 ttcatgtcca aattcccgta tgaccaaact gaaaaaacct tccttgagtt ttcgattggg
     1501 gtttttaaga gtcataacgt ttcttttcaa gccatttgct taatatcata gaaaatatct
     1561 ttgaaaatat aagaattgcc tcaattttta gtgagaatct tttacatttg taataaaatt
     1621 gttggtttgt agataattga tgaaatatca atataaggaa aaatattaaa aatcagtcga
     1681 ttaaattatg gaacgcaggg aattcatgag aaagctttgt ttaggcgcag taggtggaac
     1741 tttgatggta aagggaatga gagcacagag taaggactta gaattattaa ctccctcttt
     1801 tattccttct tcctcgggat atgatttggt tgccgttaaa ggaggc
//
LOCUS       DAORPD010000351         1844 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_11873_length_1844_cov_2.514254, whole genome shotgun sequence.
ACCESSION   DAORPD010000351 DAORPD010000000
VERSION     DAORPD010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11873_length_1844_cov_2.514254"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="PK976_04830"
     CDS             <1..699
                     /locus_tag="PK976_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005675594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HRC67245.1"
                     /translation="DAFPMDLPAIISNFYSRAGYVYLNNNQTGSITFIGTVSPAGGNL
                     KEPVTESTKKVARAFYALSQQRADSKRYPAIDPIESYSKYLEYPEVQESLRNTIADDW
                     VDKVMLVKNIMIRGREASEQINILGDDSVPLDYHLTFWKSEVIDSIILQQDAFDKIDF
                     NTPMTRQKYMLDKVIDIYETNFDFDSFEDVNPYFKRIINQLKQMNYSEFQSEQFKHFE
                     NELYKIIEERKLKK"
     gene            696..>1844
                     /locus_tag="PK976_04835"
     CDS             696..>1844
                     /locus_tag="PK976_04835"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007560284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HRC67246.1"
                     /translation="MSPLAFQKIYTHISNITKATCTLKASGVGNEELALVHGRLSQVV
                     KIIDNEVTLQVFGGTEGIPSNAEVIFYNRPPTLKVSDDLAGRFFNAYGQPIDGGPEVE
                     GEERPIGGPSVNPVRRRQPSELIATGIAGIDLNNTLVTGQKIPFFADPDQPFNQVMAM
                     VALRAEADKIILGGMGLTNDDYLYYKNVFENAGALDRIISFVNTTEDPPVERLLVPDM
                     ALTAAEYFAVDKNEKVLVLLTDMTLYADALSIVSNRMDQIPSKDSMPGSLYSDLAKIY
                     EKAVQFPSGGSITIIAVTTLSGGDITHAIPDNTGYITEGQLFLRRDTDIGKVIVDPFR
                     SLSRLKQLVIGKKTREDHPQVMNTAVRLYADAANAKTKLENGFDLTDYD"
BASE COUNT          550 a          426 c          378 g          490 t
ORIGIN      
        1 gatgccttcc ctatggacct gcccgcaatt atctcaaact tttactctcg tgcagggtat
       61 gtctatttga acaataacca aaccggaagc attacattta ttggaacggt ttcgcctgca
      121 ggcggaaatt tgaaagaacc cgttaccgaa tcaacaaaaa aagtagctcg agctttttat
      181 gcactatctc agcaaagagc tgatagcaaa cgttatcccg ccatcgatcc tatcgaaagt
      241 tattctaaat atctcgaata tcccgaggtt caagaatcat tgcgcaatac cattgccgat
      301 gattgggtcg ataaggtgat gctggtgaaa aatatcatga tccgtggacg tgaagcttcc
      361 gaacaaatca atattctggg cgatgatagt gttcctctgg attatcacct tactttctgg
      421 aaatcagaag taatagattc cattattctt caacaggatg ccttcgacaa gatcgacttc
      481 aatactccca tgacgcgcca aaaatatatg ctcgacaagg tgatagatat ttacgaaacc
      541 aatttcgatt tcgacagctt cgaagatgtc aacccctact ttaaacgaat tattaatcaa
      601 ttgaaacaaa tgaattattc cgaatttcaa tcggaacagt ttaaacattt tgaaaatgaa
      661 ttatataaaa tcattgagga aaggaagtta aaaaaatgag tccacttgca ttccagaaaa
      721 tatataccca tatcagcaat attaccaagg ctacatgcac gctgaaagct tcgggagttg
      781 gcaacgaaga actggcactg gtgcatggac gcctgtctca agtggtaaaa attatcgata
      841 atgaagtaac acttcaggta tttggtggta cagaaggaat tccttccaat gccgaagtta
      901 ttttctataa ccgtcctcct acactcaagg taagcgatga cttggcaggt cgttttttta
      961 atgcttatgg tcagcctatc gatggaggtc ctgaggtaga gggtgaagaa cgtcccattg
     1021 gtggaccttc ggtaaacccc gttagaagac gccaaccttc ggagctcatt gccaccggaa
     1081 tagctggtat agatttaaat aatacgctgg tcaccggaca gaaaattcct tttttcgccg
     1141 accccgatca acccttcaat caggtaatgg ctatggtagc tctccgtgcc gaagccgaca
     1201 agatcattct gggcggaatg ggcttgacga acgacgacta cctttactac aaaaatgtgt
     1261 tcgaaaacgc cggtgccctc gaccgtatca tcagtttcgt aaatactacc gaagatccac
     1321 ccgtcgaacg cctactggtt cctgatatgg ctcttacggc agccgaatat tttgcagtcg
     1381 ataaaaacga aaaagtcttg gtgctgctta ccgatatgac actttatgcc gatgccctca
     1441 gtatagtctc caatcgtatg gatcagatac cttctaaaga ctccatgccc ggctcacttt
     1501 atagcgattt agccaagatt tatgaaaagg ctgtccaatt tcccagtgga ggttccatta
     1561 cgattattgc agttactacc ctttcaggag gcgatattac ccatgccata cccgataata
     1621 cgggttatat caccgaaggc cagcttttcc tgcggcgtga tacagatatt gggaaggtta
     1681 tcgtcgatcc tttccgcagc ctttcacgac tgaaacaact cgtcatcgga aagaagacac
     1741 gtgaggatca tcctcaagtg atgaatacag ccgtacgtct ttatgccgac gccgccaacg
     1801 ccaaaactaa actcgaaaat ggcttcgacc ttacggatta cgac
//
LOCUS       DAORPD010000352         1841 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_11910_length_1841_cov_1.970885, whole genome shotgun sequence.
ACCESSION   DAORPD010000352 DAORPD010000000
VERSION     DAORPD010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11910_length_1841_cov_1.970885"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /gene="rplF"
                     /locus_tag="PK976_04840"
     CDS             <1..501
                     /gene="rplF"
                     /locus_tag="PK976_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HRC67247.1"
                     /translation="PDHFITVQGKLGELCQKIDPAIKVNIEGGEIRLEIDNPDDKNLR
                     AKHGLYRSLINNMVIGVYEGFTIQQEIVGVGYKVAVSGQLLEFSLGYSHSLFLELPPE
                     IKAEAVTERGKNPILTLKSHDKQLLGMVARKIRSFRPPEPYKGKGIRFVGEVVRRKAG
                     KSASSK"
     gene            524..886
                     /gene="rplR"
                     /locus_tag="PK976_04845"
     CDS             524..886
                     /gene="rplR"
                     /locus_tag="PK976_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HRC67248.1"
                     /translation="MSTKNRKLKRRQRIKTSIRKRLRGTPERPRLTVYRSNSQIYAQI
                     VDDTQGKTLIATSSLQKDFSREKMTKTQQARIVGVEIARKALEAGITAVVFDRNGFLY
                     HGRVKALADAARENGLIF"
     gene            896..1414
                     /gene="rpsE"
                     /locus_tag="PK976_04850"
     CDS             896..1414
                     /gene="rpsE"
                     /locus_tag="PK976_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HRC67249.1"
                     /translation="MANANIRRVKSSEIEFKDRLVSIQRVTKVTKGGRAFSFSAIVVV
                     GNENGVVGYGLGKAKEVTAAISKGIDDAKKNLIRVPIIKGTIPHAQEGKYSGAVVFLK
                     PAAPGTGVIAGGAMRAVLESVGIKDVLAKSKGSSNPHSVVKATINALLQLRDPYTVAQ
                     LRGVDLDTVFNG"
     gene            1438..1617
                     /gene="rpmD"
                     /locus_tag="PK976_04855"
     CDS             1438..1617
                     /gene="rpmD"
                     /locus_tag="PK976_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HRC67250.1"
                     /translation="MKRIRIKQVRSWIGKPIDQKRTLKALGLGRLNRTVEHTATPQIL
                     GMVRKVAHLVSVEEI"
     gene            1654..>1841
                     /gene="rplO"
                     /locus_tag="PK976_04860"
     CDS             1654..>1841
                     /gene="rplO"
                     /locus_tag="PK976_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HRC67251.1"
                     /translation="MDLSNLKPAKGSVKSKRRLGRGQGSGRGGTSTRGNKGAQSRSGN
                     KRKIGFEGGQMPLHRRVP"
BASE COUNT          595 a          347 c          437 g          462 t
ORIGIN      
        1 cccgatcatt tcattactgt acagggcaaa ctgggagaac tctgccagaa aattgacccc
       61 gccattaagg tgaatataga aggcggagag attcgactcg aaatagacaa cccggatgac
      121 aagaatctgc gagcaaaaca tggactttac cgctctttga taaataatat ggtgattggg
      181 gtttacgaag gttttacaat tcaacaggaa attgttggag taggctataa agttgcggtc
      241 agcgggcaac ttcttgaatt ttcgttgggg tattcgcata gcctttttct ggaacttcct
      301 cccgaaatta aggccgaagc cgttaccgaa agagggaaaa atcctatact aaccttaaaa
      361 tcgcatgata aacaattgtt gggaatggtt gcccggaaaa tccgttcctt cagaccaccc
      421 gaaccttaca aggggaaagg catacgtttt gtgggtgagg tagtaagacg taaagctggt
      481 aaatcagcct catcgaaata atttttaaaa cttcgaacgc aatatgtcaa ctaaaaatag
      541 aaaactgaaa agacgccaac ggataaaaac gagtatccgc aaacgtttaa gaggaacacc
      601 cgaaaggcca agattaacag tatacaggag taatagccaa atttatgctc aaattgtaga
      661 tgatacccaa ggcaagactt tgattgctac ctcgagttta caaaaagatt tttcaaggga
      721 aaagatgacc aaaacacaac aagctcgcat agtaggagtt gaaattgccc gtaaagctct
      781 cgaagcaggt atcacagcag ttgtttttga tcgtaatggc tttctttatc atggtagggt
      841 caaagctctg gccgatgccg caagagaaaa tggtttaatt ttttaaattt tgaatatggc
      901 taacgcaaat ataagaagag taaaatcgag cgaaatcgaa ttcaaagata gactggtaag
      961 cattcaacgt gtgacaaagg ttacaaaggg tggtcgtgct ttcagttttt ctgccattgt
     1021 tgtggtagga aatgaaaatg gggttgtggg atatgggctt gggaaagcca aggaagttac
     1081 ggctgctata tctaaaggta ttgatgatgc taagaaaaat ctcattcgag taccgataat
     1141 taaaggcacc attcctcacg ctcaggaagg gaaatatagc ggagccgtag tgtttctcaa
     1201 accagccgct cccggaacgg gtgtcattgc tggtggcgcc atgcgtgccg tgcttgagag
     1261 tgtcggaata aaagacgtac tggccaaaag caagggatct tccaatcccc atagcgtggt
     1321 gaaagctacc atcaatgctc tgttgcaact gcgcgatccc tatactgttg ctcagttgcg
     1381 cggtgtggat ctcgatactg ttttcaatgg ttgaactact ataatgcttt aaacattatg
     1441 aaaagaatta gaataaaaca agttcgaagc tggattggaa aacccatcga ccagaaacgt
     1501 acactcaagg cgttgggact tggccgtttg aaccgtacgg ttgaacatac cgctacaccg
     1561 cagattctag gaatggtcag aaaagttgct catttagttt ctgttgaaga aatttgatta
     1621 attcattgat atttatctaa aactgaaaaa aatatggatt taagtaatct gaaacctgcc
     1681 aaaggatcag taaaatcgaa aagacgtttg ggaagaggcc aaggttcagg ccgtggtgga
     1741 acttccactc gcggaaacaa gggagctcaa tcgcgctcgg gtaacaaaag aaagattggt
     1801 ttcgaaggcg gtcaaatgcc actgcatcgc cgtgttccaa a
//
LOCUS       DAORPD010000353         1833 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_11986_length_1833_cov_2.213161, whole genome shotgun sequence.
ACCESSION   DAORPD010000353 DAORPD010000000
VERSION     DAORPD010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11986_length_1833_cov_2.213161"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="PK976_04865"
     CDS             <1..492
                     /locus_tag="PK976_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HRC67252.1"
                     /translation="AFRVSSKIDSRTILDTGGADQLVGRGDMLLSLGSDLLRLQCAFV
                     DLSEIESITGYIGSQRGYPEAYALPEYYESEDEIPAITEPGERDELFEEAARIIVIHQ
                     QGSTSLLQRKLKLGYNRAGRLIDQLEAAGIVGPFEGSKAREVRIPNEQALEQYLRELN
                     NKQ"
     gene            511..1137
                     /locus_tag="PK976_04870"
     CDS             511..1137
                     /locus_tag="PK976_04870"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LolA-like putative outer membrane lipoprotein
                     chaperone"
                     /protein_id="HRC67253.1"
                     /translation="MKKYLILLLAFTPVFVQAQYSSEKAQNQLQSLAQTLKSAQNIRI
                     QFTLSQTADKASASKPNQGTLWISGNKFRLMLDGQIIVNDGKTLFTYLPEANEIQIND
                     ANSSHNELNPLTLLTSYQQNYRSKYIREEDNRMIIDLLPLNENRFYKIRIEILTSNNY
                     PSKISLYERNGTTLTYQIDRFELNKDVNSSIFSFNPADYKNAEVVDLR"
     gene            1141..>1833
                     /locus_tag="PK976_04875"
     CDS             1141..>1833
                     /locus_tag="PK976_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRC67254.1"
                     /translation="MITPPLAEILRPQSLSEFIGQEHIIQPNSILYNSIQAGKVPSMI
                     LWGPPGTGKTTLAYIISRTLNCHFFSLSAIHSGVKEVRTVIEEAEKNPSRTILFIDEI
                     HRFNKSQQDSLLGAVEKGIITLIGATTENPSFEVITPLLSRCQVYILKPLERKHLEIL
                     IEKAVRYYDTQGIHLQIAENEALLRISGGDARKLLNAIELIVNNRKTGTNSLLIDNGI
                     TQKIIQQNMAIYD"
BASE COUNT          594 a          406 c          357 g          476 t
ORIGIN      
        1 gcatttagag tgagttcgaa gatcgattca cgaactatac ttgatacggg aggagccgac
       61 caattagtgg gtcggggaga tatgttgctt tcacttggga gtgatttgct tcgtttgcaa
      121 tgtgcttttg tcgatttatc ggaaatcgag agtattactg gatacatagg cagccaaaga
      181 ggctatcccg aagcctatgc cctccctgaa tattacgaaa gtgaagatga aataccagct
      241 attaccgagc cgggcgaacg agacgaactc ttcgaagaag ccgccagaat catagtcatc
      301 catcagcaag gtagtacttc cttgctccaa agaaaattaa aactcggata taaccgggca
      361 ggccgcttga ttgaccaact cgaagccgct ggcattgtag gccctttcga aggaagtaag
      421 gcccgcgaag taagaattcc taacgaacaa gcgttggaac aatatttgag agaactcaac
      481 aataagcaat aatgcacaat ctattaaaac atgaaaaaat acctcatcct tctccttgct
      541 tttacacctg tatttgttca ggcccaatat agtagcgaaa aggctcaaaa tcagcttcaa
      601 tccttagctc aaactcttaa atcggctcaa aacatccgaa tacaattcac tctttcgcaa
      661 accgctgata aagccagtgc gtcaaaacca aatcagggaa cactctggat ttcgggaaac
      721 aaatttcgcc tgatgctgga tggtcagatc atcgttaatg atgggaaaac cctatttacc
      781 tatttgcccg aagccaacga aattcagatc aatgatgcca actcgtccca taatgaattg
      841 aatccattga cgctcttgac aagctatcaa caaaactaca gatcaaaata cattcgtgaa
      901 gaagataacc gaatgatcat cgatttattg cccttgaacg aaaataggtt ttacaaaatc
      961 aggatcgaaa ttctgacatc caataactat ccatcgaaaa tttcgcttta tgagcgtaat
     1021 ggaactactc tcacttatca gatcgataga ttcgaattga acaaggatgt caattcttcc
     1081 atcttttcat ttaaccctgc cgattataag aatgcagaag tagtcgattt gcgataaacc
     1141 atgatcactc ctccccttgc cgaaatcctg cgtccacaat cgctttccga attcatcggc
     1201 caagaacata ttatccagcc caattccatt ctatataatt ctattcaagc agggaaagtg
     1261 ccctcgatga ttttgtgggg ccctccaggc acaggtaaaa ccacacttgc ttatatcatt
     1321 tcgagaacat tgaactgcca ctttttctcg ttaagcgcta tccattcggg agtaaaggag
     1381 gttcgtacag taatcgaaga agccgaaaaa aacccaagca gaaccatatt gtttattgat
     1441 gaaatacatc gttttaacaa atcgcagcag gattctttgt taggagcggt cgaaaagggc
     1501 attatcacgc taataggagc cactaccgaa aatccttcct tcgaagtcat tacacctttg
     1561 ctttcgagat gtcaggtcta tattctgaag ccgttggaaa gaaaacatct ggaaatttta
     1621 atcgaaaagg cagttcggta ttacgacact cagggaattc acttacaaat tgctgaaaat
     1681 gaagcgctat taaggatatc gggcggagat gcccgtaagt tgttgaatgc aatcgaactg
     1741 atcgttaata atcgaaaaac tggtaccaac agccttttga tcgacaatgg aatcactcaa
     1801 aaaatcattc agcaaaacat ggccatttac gac
//
LOCUS       DAORPD010000354         1832 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_11993_length_1832_cov_2.819921, whole genome shotgun sequence.
ACCESSION   DAORPD010000354 DAORPD010000000
VERSION     DAORPD010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11993_length_1832_cov_2.819921"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="PK976_04880"
     CDS             complement(<1..500)
                     /locus_tag="PK976_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HRC67255.1"
                     /translation="MTFNSLPFLIFLPIIVITYYLLSPKWRWIMLLAASYLFYMWWNP
                     AYIILILVSTLISFYSSQAIERQTSKKGKKKYLYLCLIINLGILFTFKYYGLFSQAFT
                     SIADIFGVKAQLPFLNLLLPVGISFYTFQTLSYTLDVYFGKQKAETHLGYFALFVTFF
                     PQLVAGP"
     gene            complement(519..>1832)
                     /locus_tag="PK976_04885"
     CDS             complement(519..>1832)
                     /locus_tag="PK976_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67256.1"
                     /translation="GMAMILTPGFLIAHGIAILTGLPADGFSPPYQWAILLTNLLFLG
                     LGIYFTLKTLFRYFDSITSVITIVMLVLATNVLAYVYLHPGFSHIFTFGLMGMLLYVT
                     DTFYRKPSIGLAMKAGLLMGFISLIRPSNTIVGLLFLLWGTFSVSDLKKRFQFFIKNY
                     HLTLIIAAGIVIVWLPQMIYWKWVSGRFFLYTYGLKGERFFFGNPQIVNVLLSYRNGW
                     LVYSPLFFLALLGFIFMIKNKNRQVLAIGFYFIGLIYIISSWWCWWYGGSFGARSLID
                     AYPALAFPLAAFFEKAGKTPWWLQILLIIVCGYLIHLNYYQSYQYSKGIIHWDGMSKE
                     AYWRSIKEKHPPKDYYDLFVIPHTPYAKKGIYFEHDTLNIQRPPSLQEKEFIEKIKTN
                     IRINKEWMQVIEEKAKRKGISVDSMLTLDARHIYKIRYHEKVPKN"
BASE COUNT          618 a          335 c          362 g          517 t
ORIGIN      
        1 ggtccggcca ccagttgagg gaaaaaagtc acaaaaagag cgaaataccc cagatgagtt
       61 tctgcctttt gcttcccaaa atatacatcc aatgtatagc ttaaggtttg gaaggtgtaa
      121 aatgaaattc ctaccggaag caataaattc agaaaaggca gttgggcttt caccccaaaa
      181 atatcggcaa tactcgtgaa ggcttgagaa aataatccgt aatatttaaa agtaaataaa
      241 attcctaaat tgataatcaa gcatagataa agatattttt ttttcccttt ctttgaggtt
      301 tgtctttcaa tggcttggct cgaataaaaa ctaatcagtg ttgaaacaag aattaaaatg
      361 atataagctg gattccacca catataaaac aggtagctgg ccgcaagtag cattatccat
      421 cgccactttg gtgatagaag ataatatgtg atgacgataa tgggcagaaa aataagaaaa
      481 ggaagggaat taaatgtcat gttgaattcc taattttttt aatttttagg aactttttcg
      541 tgatacctga ttttgtaaat gtgtcgagca tctaaagtga gcatagaatc aacagatatt
      601 ccttttcgtt tggctttttc ttctataact tgcatccatt ctttattaat ccggatattg
      661 gtttttattt tttcgatgaa ttctttttcc tgtaaagatg ggggtctttg aatattcagc
      721 gtatcgtgtt cgaaataaat tcctttcttg gcataagggg tatggggaat tacaaataaa
      781 tcgtagtaat cttttggagg atgcttttct tttatggatc tccaatatgc ttctttcgac
      841 attccatccc aatgaataat tccttttgaa tattgataag actggtagta attcagatgg
      901 atgagataac cgcaaacaat aatgagcaat atttgaagcc accagggggt tttccctgct
      961 ttttcgaaga aggcggctaa tgggaaagcc agagcggggt atgcatcgat caagctacga
     1021 gctccaaatg aacctccata ccaccagcac caccacgatg aaataatgta tataagtcca
     1081 ataaaataaa aacctatggc taaaacctgt cggtttttgt tctttatcat aaaaataaaa
     1141 ccaagcaatg caaggaaaaa caggggtgaa taaaccagcc atccgttgcg atagcttagt
     1201 aaaacgttga cgatttgagg attaccaaaa aagaatcttt cacccttcaa tccataagta
     1261 tataagaaaa acctgccact tacccatttc cagtaaatca tttgaggcag ccatactata
     1321 acaattccag cagcgattat caatgttaga tgataatttt ttatgaagaa ctggaatctt
     1381 tttttcaggt cgctcaccga aaatgtcccc caaagtagga aaagcagtcc aacaatggtg
     1441 ttactgggtc gtatcaaact gataaaaccc atgagcaagc ctgccttcat cgccaatcca
     1501 attgatggtt tgcgatagaa ggtatcggtt acgtacaaga gcatccccat cagtccgaaa
     1561 gtaaagatgt gcgaaaagcc aggatgcaga taaacatacg ccaatacgtt agtggcaagc
     1621 actaacataa cgatggttat caccgaggta atactgtcga agtaacgaaa cagggttttg
     1681 agtgtaaaat aaattcccag tcccaagaaa agtaaattag tcaggagaat tgcccattga
     1741 tagggaggtg aaaatccatc ggcaggcaag cccgtaagta tggctatgcc atgggcgatg
     1801 agaaaacctg gcgtaagaat catagccatg cc
//
LOCUS       DAORPD010000355         1831 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_12008_length_1831_cov_2.425113, whole genome shotgun sequence.
ACCESSION   DAORPD010000355 DAORPD010000000
VERSION     DAORPD010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12008_length_1831_cov_2.425113"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..>1831)
                     /locus_tag="PK976_04890"
     CDS             complement(111..>1831)
                     /locus_tag="PK976_04890"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HRC67257.1"
                     /translation="DSIYFVDNNNLNIELWINEGHKYYFRNITWVGNTKYSSDVLNAY
                     LKIRKGDVYNQQMLQTNLSYNPNGFDVSSLYLDDGYLFFNIQPVEVNVENDSIDLEIR
                     IYEGKQATLNRVTVKGNDRTSDHVIIRELYTKPGQLFSRNDIIRTQRELAQLRYFNPE
                     KLGVDYTPNPEDGTVDLEYTVEETSSDQIELSAGWGYGRLVGTLGLSFNNFSMRRIFD
                     KNAWKPIPSGDGQKLSLRFQTYGVGYTSYSASFTEPWLGGKKPNAFTVSYYHSKYTNG
                     LKSSDTLYASFIIDGLGFSLGKRLEWPDDYFQIYHSINLQRYRLINYPNIFTFGTGNG
                     EYNNFSYTISFGRSSDDSPIFPRRGSTVNLSLELTPPYSLFSNKDYSSMSAEEKYKWI
                     EYHKWKFYGNWYSQLVGNLVLATKYRFGFLGYFNKEIGVTPFERFYVGGDGLSGYGSL
                     DGREIIALRGYSNESITPEYFENNGVGGTIFSRYVFELRFPLSLNPNSTIYALAFMEG
                     GNTWNKFTSFNPFKVYRSAGLGLRIFLPMFGVLGLDYGYGFDEVPGIPSANGGQFHFS
                     INQSID"
BASE COUNT          532 a          374 c          376 g          549 t
ORIGIN      
        1 caatagccag cattatggcc gtaaatagaa gagcaagcct ttttcttatc ataattcctc
       61 ctttgatttg aattttgaaa cgaatttaca aattttatgc cttatattca ttaatcaatg
      121 gactgattga tggagaaatg gaattgacct ccatttgcgc tggggattcc gggtacctcg
      181 tcgaatccgt acccatagtc gagtccaaga acgccaaaca taggcagaaa tatcctcaat
      241 ccaagaccag cacttcggta aaccttgaaa ggattgaagc tggtgaattt attccatgta
      301 ttgcctcctt ccataaaagc aagggcatag atggttgagt tggggttgag cgataaggga
      361 aaacgcaact cgaaaacata gcggctgaaa atcgtgcctc ctaccccgtt gttttcgaaa
      421 tattcaggag taatgctttc gttggaataa cccctcagag caatgatttc tcgtccatcc
      481 aaactgccat atcctgagag cccatctcca ccaacataga atctttcgaa aggagtaacg
      541 cctatttctt tgttaaaata gccgagaaat ccaaagcggt atttcgttgc caataccagg
      601 ttgcctacca gttgagagta ccagttacca tagaatttcc atttatgata ttcaatccat
      661 ttatattttt cctcggcgct catactgcta tagtccttat tgctaaagag gctatatgga
      721 ggagtgagtt cgaggctgag gtttacggtc gaacctcgtc gcgggaatat gggagaatca
      781 tcgctacttc ttccgaacga aatggtatag ctaaaattat tatattcgcc attgccggtt
      841 ccaaaagtaa aaatattggg gtagtttatt aatctgtaac gttgcagatt gatggagtga
      901 taaatctgaa aataatcgtc gggccattcg agtcgtttac ccaaactaaa gccaagtcca
      961 tctataataa aggaggcata aagcgtgtcg ctacttttga gaccattggt atatttggag
     1021 tgataatagg aaacggtaaa ggcattaggt tttttccctc ctagccaggg ttcagtaaat
     1081 gaagcagaat agctggtata tccaacgcca taggtttgaa atcggagact gagtttttgt
     1141 ccatctcctg atggtattgg tttccatgca ttcttatcga aaattctcct catggaaaag
     1201 ttattgaacg acaaaccgag tgttccaacc aatcggccat aaccccagcc agcagaaagt
     1261 tcgatctgat cgctcgatgt ttcctctacg gtatattcca ggtcaactgt tccatcctcg
     1321 ggattaggag tgtagtccac gcccagtttt tcaggattga aatagcgcaa ttgagctaat
     1381 tcacgttgtg tacgaatgat atcgttacga ctaaatagct gaccaggttt ggtatagagt
     1441 tcacggataa tgacatgatc gctggttcta tcgttacctt tcacggttac cctgttcaat
     1501 gtggcttgct tgccttcata tatcctgatc tcaaggtcga tggaatcgtt ttctacattc
     1561 acttctactg gctgaatatt aaaaaacagg taaccatcat cgagatataa cgagctcacg
     1621 tcgaatccat taggattata agaaaggttg gtttgcagca tttgctggtt ataaacatca
     1681 ccctttctaa ttttcaggta agcattcaat acatcagagg aatatttggt attacccacc
     1741 caggtaatat tgcggaagta atatttatgg ccttcattga tccatagctc gatattcaga
     1801 ttattgttgt ctacaaaata gatggaatct t
//
LOCUS       DAORPD010000356         1831 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_12009_length_1831_cov_2.390766, whole genome shotgun sequence.
ACCESSION   DAORPD010000356 DAORPD010000000
VERSION     DAORPD010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12009_length_1831_cov_2.390766"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1831)
                     /locus_tag="PK976_04895"
     CDS             complement(<1..>1831)
                     /locus_tag="PK976_04895"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRC67258.1"
                     /translation="DLNRTGSETNITRDKIDKLPTIGRNLTDYTRLTPQARVQETSDG
                     PNLTVAGMNNRYNAIFIDGAVNNDVFGLAGSGTNGGQTGISPFSMDAIDQLNIAIAPY
                     DIKLGGFAGAGINAVTRRGTNEIEGSAYYYFRNQDLAGKTPTDDSNIERKKLPDFSSK
                     TYGVRLGGPIIKDKVFFFGNVEIQKDETPQPFEFSTYEGNSDVDKISQLIEKVKSYGY
                     DPGGFQGVIKKLDGTKIFGRVDWNINQNHKLTLRHQYTKGTKTEPGYQTNTNITFENA
                     GIYFPSTTNSSALELKSIFGNKFANNLIVGLTFVRDDRDPMGQNFPYVSIRDGKGSIN
                     LGSEQFSTANQLDQDIITLTDNFEWYSGKHTLTFGTHNEFYNMYNLFIRQNFGVYTYN
                     SIDDFLNNDTATQYDRTFSMTDGKTGDGSSAATKFKALQLGFYAQDEYQALDNLKLTF
                     GLRVDIPMFLDDPIVNKDFNNNIIPVLEQNGWDLQGAQTGQMPDVHLLWSPRFGFNWD
                     VFNDQSTQVRGGIGIFTSRIPYVWPGGVYNNNGLMLGGMRVTYVSNPTSPYLIFNPDW
                     NNQPGWKPGDPVTPSGQIDLFAKNFKFPQVWHMSLAVDQKLPWD"
BASE COUNT          495 a          371 c          397 g          568 t
ORIGIN      
        1 atcccaaggt agtttttggt cgacggccaa actcatatgc caaacctgag ggaatttgaa
       61 attcttagca aataggtcaa tctggcctga aggtgtaact ggatctccgg gtttccatcc
      121 gggctggttg ttccaatcgg gattgaaaat cagataaggg ctcgtagggt tcgaaacata
      181 ggttaccctc atgccgccaa gcataagtcc attgttgttg taaacaccac ctggccaaac
      241 ataaggtatg cgggaagtga aaatacctat acctcctcta acctgagtac tttgatcatt
      301 gaatacatcc cagttgaatc cgaatctggg cgaccataac agatggacat caggcatttg
      361 accggtttgt gccccttgca aatcccatcc attctgttct aaaacaggaa tgatgttatt
      421 attgaaatct ttgtttacaa tgggatcgtc taaaaacatg ggaatatcaa ccctgaggcc
      481 aaaggtaagt ttcaaattat ccagagcttg atattcgtcc tgagcataga atcccaactg
      541 aagggctttg aattttgtag ctgcactcga accatcaccc gttttcccat ctgtcatcga
      601 aaatgttcta tcatattgag tagctgtatc attgttcaag aaatcatcaa tgctgttata
      661 ggtataaact ccgaaatttt gacggatgaa gaggttatac atgttataga attcgttgtg
      721 cgtaccaaat gtaagggtat gttttccaga gtaccattcg aaattatcgg taagagtgat
      781 aatatcctga tcgagctgat tggctgtgga aaattgttca gaaccaagat tgatgctgcc
      841 tttaccgtcg cgaatgctca cgtaagggaa attctgtccc ataggatcgc ggtcatctct
      901 aacaaaggta agtcccacaa tcaggttgtt ggcaaatttg tttccgaaga tacttttcag
      961 ttctaaagcc gatgaattgg tggtagatgg aaaatatata cccgcatttt cgaaagtgat
     1021 gttagtattg gtttgatacc caggttcggt ttttgttcct tttgtatatt gatgtcgaag
     1081 ggtaagctta tggttttgat tgatgttcca gtcgacacgt ccaaatattt tggtgccgtc
     1141 gagttttttt ataactccct gaaatccacc cgggtcataa ccatacgatt ttaccttttc
     1201 gattagttga ctaattttat ctacatccga attgccttca taagtagaaa attcgaaagg
     1261 ctgaggagtt tcgtcctttt gaatctcgac gttgccaaaa aagaaaactt tatccttgat
     1321 gatagggcca cctaagcgaa cgccataagt tttactcgaa aaatcgggta gtttctttct
     1381 ttcgatgttg gaatcgtcgg tgggagtttt tcctgcgagg tcttgattac ggaaataata
     1441 ataggccgaa ccttcaattt cattagttcc tcttcgagtt acggcattga ttcctgcacc
     1501 ggcaaaacca cccaatttga tgtcgtaagg agcaatagca atattcaact gatcgatggc
     1561 atccatacta aaggggctta taccggtttg tccaccgttg gttccacttc cggctaatcc
     1621 gaatacatcg ttgttaacgg caccatctat aaatatggca ttgtagcggt tgttcatgcc
     1681 agctaccgta agattgggtc catcgctggt ttcctgaacc cgggcttgag gcgtcaatcg
     1741 agtgtaatcg gttagattgc gcccgatggt tggtaattta tcgattttgt cacgtgttat
     1801 attggtttca ctaccggttc gatttaaatc g
//
LOCUS       DAORPD010000357         1830 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_12025_length_1830_cov_2.329577, whole genome shotgun sequence.
ACCESSION   DAORPD010000357 DAORPD010000000
VERSION     DAORPD010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12025_length_1830_cov_2.329577"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(488..961)
                     /locus_tag="PK976_04900"
     CDS             complement(488..961)
                     /locus_tag="PK976_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67259.1"
                     /translation="MNLLSGCDRFSCFTRLANLTLVFCLIVSFWACTDDNDISPIPDV
                     YVNIVINPASIEYGNLNIPGNYALIKGGYRGIIVYHVIDNQYMAYERTCTYDWEKPCS
                     KLIIDASGLIARDTCCKSSFLLIDGSPMEGSLATIPLKQYKTMYDPNFGRLYITN"
     gene            1051..1320
                     /locus_tag="PK976_04905"
     CDS             1051..1320
                     /locus_tag="PK976_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67260.1"
                     /translation="MMLVDISCSTRESQTAKQQKEIEMRKEERRKKEEAAYQSKIERH
                     EKIQSEEGRKLIQQANEHRNKLDKQNNKKKFSLKNLFGKDEPACH"
     gene            1413..>1830
                     /locus_tag="PK976_04910"
     CDS             1413..>1830
                     /locus_tag="PK976_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67261.1"
                     /translation="MEDLIVLLIFIAWIGYTFYKELKKHKKTVGKPPTLPVDIPEQAD
                     KRRITPQMEEFDYDTDFEGGIPVTEAKENVIMASPEVSRAETSSPIQFIPLINQIHRP
                     DFESIEYVRDEQTKIHELMKEQPEDEASKEFSFDLRQ"
BASE COUNT          621 a          322 c          361 g          526 t
ORIGIN      
        1 acggtggtac ttttgtaaaa tattgatatg caattatagg atcaggagtt ctgaatttac
       61 aaaatcttac gaattgcagg ttttcaatgc ttttggttct tgcatttata atagaaagat
      121 atcacgtaac gattatcagt taaatctttc taatcagagc tcggatattt atttttatcg
      181 ggcttacgat gaatcaggaa ctttgggaac tggtaaactg ataattagga aataaggaag
      241 gactgcattg taattgttga atctctaaag agactaataa tttgtcaatg ctcaaatgac
      301 tttagtcttt tttgggctaa ttatacgtga tgcgagagaa aagttcccgc aaaatgacac
      361 aatttctgaa ctttcaagac ttttactcac catcccttat ccacctatac ctatttgcca
      421 tcttctatcc cgtacccgtc ataatcctat tcatgagcag agtgtatata aaaacataca
      481 gtatgactta attcgtaata tacaatctgc caaaattcgg gtcatacatc gttttgtatt
      541 gttttaatgg aatagtggct aaacttcctt ccatgggcga accatctata agtagaaaac
      601 tcgatttgca gcatgtatcg cgggcaatca atcccgaagc atctattatt aatttcgaac
      661 agggtttttc ccaatcgtaa gtgcaggttc tttcgtatgc catgtattga ttgtcgatta
      721 catgataaac gataattcca cggtatccac ctttaatcaa tgcataattg ccaggtatat
      781 tcaaattgcc atactctatc gaagcaggat tgattacaat gttcacatac acatcgggaa
      841 tgggcgagat gtcattatcg tcggtacaag cccaaaagga aacaatcaag caaaatacaa
      901 gggttaggtt tgctaacctg gtgaagcatg aaaatctgtc gcaaccgctt aataaattca
      961 tattgattta ttttgtttaa gtttgcaaag ataatgccct tttatatgcg aggaatgaga
     1021 tttcggcgga taatcataat gatgtttctg gtgatgctcg tagatatttc ttgtagcacc
     1081 cgagagagtc agaccgcaaa acagcaaaag gaaatcgaaa tgcgaaagga agaaagacga
     1141 aagaaggaag aggcagctta tcagagtaaa attgaaagac atgaaaaaat tcagagcgaa
     1201 gaagggcgga aattgattca gcaagccaat gaacatagaa acaagctgga caagcaaaat
     1261 aacaaaaaga aattttcgtt gaaaaatctg tttggcaaag atgaaccggc ttgccattaa
     1321 catacatcac acgaaaagtt tatttggttt tacttctttc tgtgaggaga ggatatcaag
     1381 aatttattta attttaccat caaatttgct atatggaaga tctgatcgtt ttgctcatat
     1441 ttattgcatg gataggctat acattctata aggaattgaa gaaacacaag aaaacggtgg
     1501 ggaagcctcc gaccttgcct gttgatatac ccgaacaagc agataaaaga agaatcaccc
     1561 cccagatgga agaattcgac tatgatacag acttcgaagg aggaattccg gttacagaag
     1621 caaaggagaa tgtgataatg gcatcccccg aagtttcaag ggccgaaacc agttcaccca
     1681 tccaatttat acccctgata aatcaaattc atcggcctga ttttgagagc attgaatatg
     1741 tacgtgatga gcaaaccaaa attcatgagt taatgaagga acaacccgag gacgaagcct
     1801 ctaaggagtt ttctttcgat ttaaggcaag
//
LOCUS       DAORPD010000358         1830 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_12029_length_1830_cov_1.860845, whole genome shotgun sequence.
ACCESSION   DAORPD010000358 DAORPD010000000
VERSION     DAORPD010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12029_length_1830_cov_1.860845"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="PK976_04915"
     CDS             <1..375
                     /locus_tag="PK976_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine radical domain-containing protein"
                     /protein_id="HRC67262.1"
                     /translation="PLSEGISPVQGADHHGPTAVIRSCAKLDHLRTGGTLLNQKFTPS
                     FFKNHEAIQKLTALVRTYFRLDGHHIQFNVVNAEILRDAQREPEKYRDLIVRVAGYSD
                     YFNDLTPELQEEIIRRTEHETM"
     gene            461..>1830
                     /locus_tag="PK976_04920"
     CDS             461..>1830
                     /locus_tag="PK976_04920"
                     /inference="COORDINATES: protein motif:HMM:NF024724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HRC67263.1"
                     /translation="MEFEEIKTKADAKYLALVKVIKPYLHRNDTAVLRKAYETSLVHL
                     GEIINEARQPLVLQQLDIALILAHRMGMGPQTVLPAFVVEMLRFGKIEPTQISSTFGE
                     VTLKITQGLLSIEKLNLSKVSTGSENFRELLISLSNDIRGIFYLMARNLYDLNHYSSL
                     PTASQQLIIQLARDVFIPFAHRLGLYNIKTQLENDVMRYCEPEIYRNIAQKMEELRPS
                     QQSFFTDFIIPIREALSEAGIECEFKWRFKTIPSIYQKIKKQNVDIQHVYDLFAVRII
                     LTGQLSDEIAECWRVYSIVSNIYPPDTSRLRDWITRARPSGYESLHTTVMTPEGKFVE
                     VQIRTRRMDEIAEKGDAAHWHYKEAGRKSEIDTWLREIREVLESYPGEGADPLHTHQP
                     LPSSTHIFVLTPAGDVKELPQGASVLDFAFEVHSSLGASCTGARVNGKVVPLKHRLSN
                     GDKIEIL"
BASE COUNT          540 a          406 c          377 g          507 t
ORIGIN      
        1 cctctttcgg agggtatttc gcccgtgcag ggagccgatc atcatggtcc aaccgccgtt
       61 atccgttcct gtgccaaatt agaccacctg cgaaccggcg gtactctctt gaatcaaaaa
      121 tttactccct ccttcttcaa aaaccatgaa gccatacaaa aactaactgc cctggtaaga
      181 acatatttcc gattggatgg tcatcatata caatttaatg tggtgaatgc cgaaattttg
      241 agggatgctc agcgtgaacc cgaaaaatac cgcgatttga tagtgcgcgt agccggttat
      301 tccgattatt tcaacgacct gactcccgaa ctccaggaag aaatcatcag aagaactgaa
      361 cacgaaacca tgtaattcat tttgtcgttt taattattga cctgatgaca gctttttcgg
      421 ggctttcgta ttttctttat ttccaaacta attttgcatc atggaattcg aagagataaa
      481 aaccaaagcc gatgcaaagt atctggcact tgtcaaggta atcaaacctt acctgcacag
      541 aaacgacact gcagtattgc gcaaagcata cgaaacctca ctcgtgcatc tgggagaaat
      601 aatcaacgaa gcacgtcaac ccttggttct tcagcaactc gatattgcac tcattcttgc
      661 ccatcgaatg ggaatggggc cgcaaaccgt gctacctgcg tttgtggttg aaatgttgag
      721 atttgggaaa atagaaccca ctcagatttc ttcgaccttt ggcgaagtta ccctgaaaat
      781 tactcaggga ttgttatcca ttgagaagct taacctatcg aaagtttcca ccggttcgga
      841 aaatttccgt gaacttctca tcagcctttc caatgatata agaggcatct tttatctaat
      901 ggccagaaac ctctatgatt tgaaccatta ttcttctttg cctacggcat ctcaacaatt
      961 gatcatacaa ttggcgagag atgtttttat accctttgca catcggcttg gattgtataa
     1021 tatcaagact cagttagaga atgatgtgat gcgatattgc gagccagaaa tctatagaaa
     1081 tattgcacaa aaaatggaag aacttcgtcc gagccaacaa tcctttttta ccgattttat
     1141 tattcccatt cgcgaagctt tatcggaagc aggaatcgag tgtgaattca aatggagatt
     1201 caagacaatt cccagtatat atcagaaaat aaaaaagcaa aatgttgata tccaacatgt
     1261 ttacgatctc tttgccgttc gcatcatact tacaggccaa ctcagcgatg aaatagccga
     1321 gtgctggaga gtttatagca tagtaagcaa tatttatcca cccgatactt cacgcctgcg
     1381 tgattggata acacgcgccc gaccgagtgg ctacgagagt cttcatacta ctgtaatgac
     1441 tcccgaagga aagtttgtgg aagtacagat tcgcacccgt cgtatggacg aaatcgctga
     1501 aaaaggtgat gctgcccact ggcattataa ggaagcaggt agaaaatcgg agatagatac
     1561 ctggttacgg gaaattcgtg aagttctgga atcttatcct ggagagggag ccgatccctt
     1621 acatactcat caaccattgc cgtcttctac tcatattttt gtgcttactc ctgcaggtga
     1681 tgtaaaagaa ttgcctcagg gtgcaagtgt cctcgatttt gccttcgaag tacattcgag
     1741 ccttggagcc agttgtacag gagcccgggt aaatggcaaa gtagttccct taaaacatcg
     1801 attgtccaat ggcgataaaa tagaaattct
//
LOCUS       DAORPD010000359         1829 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_12039_length_1829_cov_2.236753, whole genome shotgun sequence.
ACCESSION   DAORPD010000359 DAORPD010000000
VERSION     DAORPD010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12039_length_1829_cov_2.236753"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1258)
                     /locus_tag="PK976_04925"
     CDS             complement(<1..1258)
                     /locus_tag="PK976_04925"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRC67264.1"
                     /translation="MDKKSHSQPGIKYIHILRIAGDALMVNKTRTLLTALGIIFGVAA
                     VIAMMAVGNGARKEILDQMKLVGVNNIIITPKSDITQKNEEEAEQSKNLKKGFSAGLS
                     MSDAEGIKKIIPGVITVSPEVTYEVMGIHEGIGRTLKISGVTPDFFNTFGLKLENGVI
                     FSEKEMEQSSPVCVIGSEIRAKIFPNKDPLGQYIKCSGIWLKVIGVLEYRPVSEKSSE
                     SLGISNYNQHVYAPIKTVLNRIKDRSLITSKLLSGANVYNGGDFVVIESSSGAKAASF
                     NQLDKIVVQVKESSMLSTSSEIIKRILLRKHNEIEDFEVIIPELLLKQEQRTKDIFNI
                     VLGAIASISLIVGGIGIMNIMLASVLERIKEIGVRQAIGARRQDIVLQFLSEATLISI
                     AGGFIGIILGFILSKVIMKLAGILTII"
     gene            complement(1251..>1829)
                     /locus_tag="PK976_04930"
     CDS             complement(1251..>1829)
                     /locus_tag="PK976_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67265.1"
                     /translation="KKMGVGAQISTWDNIVATLPDLTKMISKTYVNEIDISKVKTSQK
                     AEISIDAFPNKKLTGLVDEVANIGEQIQGSNAKVFEVVINVNEFDSILRPAMTTKNVI
                     ITREFQNVLYLPIEAVHTEGKVTFVYKDNKKVEISTGESNENYIIVKNGLKEGDAVYI
                     SIPKETDHWPVARLSKSESTDKPKDGRDKSNG"
BASE COUNT          460 a          403 c          325 g          641 t
ORIGIN      
        1 atataatcgt cagaattcct gccagtttca tgatgacttt cgataaaata aagcccagaa
       61 tgattcctat gaaaccacct gctatgctga tcaaagtagc ttcggaaaga aattgcagta
      121 caatgtcttg cctgcgagct ccaatagcct gccttacgcc aatctctttg atacgttcca
      181 gaaccgaagc cagcatgatg ttcataatgc ctattccacc aacgattaat gaaatactcg
      241 ctatggctcc caatacaatg ttgaagatat ctttggttcg ttgttcctgt ttgagaagca
      301 attcgggtat gattacctcg aagtcttcga tttcattatg tttacgcaga agtatccgct
      361 tgatgatttc actactcgtg cttaacatgc tgctttcctt tacctgaact acaattttat
      421 ctaattggtt aaaggaagct gcttttgctc cgcttgaact ttcgataact acaaaatcac
      481 ctccattgta aacatttgct ccgcttaaaa gtttggaggt aatgagcgag cgatctttta
      541 ttcggttcaa tacggttttg atgggagcat atacatgttg attataattg ctgatgccca
      601 gactttctga acttttttct gaaaccggcc tgtattctaa tacacctatc accttcaacc
      661 atataccgct acattttata tattgtccca agggatcttt attggggaaa atcttagccc
      721 taatttcaga acctattacg caaacaggtg aagattgttc catttccttt tccgaaaaaa
      781 ttacgccatt ttcgagcttc aatccaaacg tattgaaaaa atcaggtgtt actccactga
      841 tcttcaatgt tcttcctatg ccttcatgaa ttcccattac ttcataggtt acttcaggac
      901 ttaccgtaat cactccagga attattttct tgattccttc ggcatcactc attgataatc
      961 ctgctgaaaa tcctttcttg agattcttgc tttgttctgc ttcttcttcg tttttctgtg
     1021 taatatccga tttgggtgta atgatgatat tgttgacccc aactaatttc atttgatcca
     1081 gaatttcttt acgcgctcca ttgcccacag ccatcattgc gatgacggcg gcaacaccaa
     1141 agataatccc cagcgcagta agcagagttc tggttttgtt taccatcagg gcgtcacctg
     1201 caatccgtaa aatgtgaatg tatttgattc ctggctggga atgacttttc ttatccattt
     1261 gatttgtccc ttccatcctt cggcttatct gtggattcac tcttgctcaa gcgggcaaca
     1321 ggccagtgat cagtctcttt gggaatgctg atgtaaactg catccccttc ttttagccca
     1381 ttttttacga tgatataatt ttcgttgctt tcgcctgtag aaatttcgac ttttttattg
     1441 tctttatata caaaggtaac tttcccctct gtatgaacag cctctatagg caaatacaat
     1501 acattctgaa attctctggt tattataacg tttttggttg tcatggcagg acgcaatatg
     1561 ctgtcgaatt catttacatt aatcactact tcgaaaactt tggcatttga tccctgtatc
     1621 tgctcgccaa tattggctac ttcgtcaact aggcccgtga gttttttatt ggggaaggca
     1681 tctatgctaa tttcggcttt ttggctggtt ttaactttcg agatatcgat ttcgttgacg
     1741 taagtttttg aaatcatttt tgtgagatcg ggaagggtgg ccacaatgtt gtcccatgta
     1801 ctgatttgag cgcccacacc cattttttt
//
LOCUS       DAORPD010000360         1827 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_12057_length_1827_cov_2.086343, whole genome shotgun sequence.
ACCESSION   DAORPD010000360 DAORPD010000000
VERSION     DAORPD010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12057_length_1827_cov_2.086343"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /locus_tag="PK976_04935"
     CDS             complement(<1..800)
                     /locus_tag="PK976_04935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRC67266.1"
                     /translation="MTLRSKRIIRITMTIAAGVVILPLIFYPKIKPLWNKASEGGQLM
                     PQRGAGRGNQPLFASGYVIIPVQMNEMIRSTGTLLPDEEVELTFETQGKVVGIYFDEG
                     RRVKKGELLAKLNDAPLQAQLLKLQAQHKLTEEKVFRQKQLLDRDAISRESYDQVATE
                     LQTIEADIKLLEARISETELRAPFDGIIGLRLISEGAYATTQTPLARLVKISPLKLEF
                     SIPERYAGDVSPGFPVKFSIDGISKEFQADVYAIDPKIEINTRTIVVRA"
     gene            complement(1152..>1827)
                     /locus_tag="PK976_04940"
     CDS             complement(1152..>1827)
                     /locus_tag="PK976_04940"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HRC67267.1"
                     /translation="LMNLIEIVSAAIPLVIAGGLIGIIKYKFPKGNISSLKYAFIWGI
                     IAGLASVFIQYLAHWMNLDSLRSLKRMTFFAFIVVGGGQEFFKFLVLRFYALPKNSFK
                     GSSDGIIYSIIIGMACCFVILIYYILSPTMKNLPYYNPMITTMGLVSVIFAIFMGFFT
                     GLGKARKNKFVDSMTGLFAAAFIHGLYKFCFYTEEYTLGVIAAIAELIIAIILIIISV
                     RTQEVS"
BASE COUNT          577 a          384 c          334 g          532 t
ORIGIN      
        1 gcccttacca ctatagttcg ggtattgatt tcaatcttgg ggtctatggc ataaacatcg
       61 gcctgaaatt cttttgaaat tccgtcgatg gaaaatttaa ccgggaatcc tggtgaaaca
      121 tcgccagcat aacgctcggg aatagaaaat tcgagtttca agggactgat ttttactaat
      181 cgagctaggg gtgtttgagt ggtagcgtag gcgccttcgc ttatcagacg caatccgatg
      241 attccatcga aaggagcacg cagctcggtt tccgaaattc gcgcttctaa gagtttaatg
      301 tcagcttcta tggtctgcaa ctcagtagct acttggtcgt aactttcacg gctaatggca
      361 tcccggtcta ataattgttt ctggcgaaaa accttttctt cagtcaattt atgttgagcc
      421 tgtagcttca acaattgagc ctgcagaggt gcatcattca atttggctaa aagctcaccc
      481 ttcttcaccc ttctgccttc atcaaaataa attcctacaa ctttgccttg tgtttcgaaa
      541 gttaattcta cttcttcatc aggaagcaat gtacccgtgc tacgtatcat ttcgttcatc
      601 tgaaccggaa tgatgacata accactcgca aacaaaggtt gattgcctct tccagcacca
      661 cgctgcggca tcaattgacc accttcagaa gctttattcc atagtggctt gatcttggga
      721 taaaaaatca agggaagtat gaccacccct gcagcaatcg tcatcgtaat ccgtattatt
      781 ctcttcgatc ttaatgtcat aaaaacctca ttatttgtta tagaagaaca tattcagggg
      841 acttcaaatt tcgtaatatt attaaagaag agctatggac aacccctcca atagtatcta
      901 cctcatatat agttaattga actcttattc taatttcaat gaatggtgaa aaatattaca
      961 aaatcttcat ctttggatat tatttcaaaa gaatggttta ttgaaaaaag ggaaaaatgg
     1021 catcccatga ttcagcatcc cgaaccatgg agccggttgg ttcattcgaa tttgatataa
     1081 ccccaaaccc aatttgctct tgacgaaaga tttgcatagg cgttttaacc atacacaaac
     1141 actttactgt atcaagaaac ttcctgggtt ctcaccgaaa ttatgattaa aattatggct
     1201 atgatcaatt cggctatagc agcaataact cctaaagtat attcctcagt gtagaaacaa
     1261 aacttataaa gtccatgaat gaatgcggct gcaaacaatc ctgtcattga atcgacgaat
     1321 ttatttttac gagccttgcc caagccagta aaaaatccca tgaatatggc aaaaataaca
     1381 cttaccagac ccatagtggt aatcatgggg ttataatatg gcaggttttt catagtaggg
     1441 ctcagtatat agtatattag aataacgaag cagcatgcca tgccaataat gatagaatag
     1501 ataatgccat ccgatgaacc tttaaaacta tttttaggca aggcataaaa acgcagcacg
     1561 agaaatttga aaaattcctg tcccccacct actacaatga aggcaaaaaa agtcattctt
     1621 ttgaggcttc gcagcgaatc gagattcatc caatgagcca gatattgaat gaacaccgag
     1681 gcaaggccgg ctattattcc ccagataaat gcatacttca atgaagaaat attgcctttg
     1741 ggaaatttat atttaatgat accaatcagt ccccccgcaa tgacgagtgg tatagctgca
     1801 gaaacgattt caattaaatt catgagt
//
LOCUS       DAORPD010000361         1822 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_12108_length_1822_cov_2.113752, whole genome shotgun sequence.
ACCESSION   DAORPD010000361 DAORPD010000000
VERSION     DAORPD010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12108_length_1822_cov_2.113752"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..1004)
                     /gene="pyrB"
                     /locus_tag="PK976_04945"
     CDS             complement(96..1004)
                     /gene="pyrB"
                     /locus_tag="PK976_04945"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HRC67268.1"
                     /translation="MKNKSLISINDFCKEEYLQILDLATYFEKNPNQKILEGFVVATL
                     FFEPSTRTRLSFESAVNRLGGRVIGFSDAALSSTTKGESLADTIRTVSNYADLIVMRH
                     PIEGSARWASQVASVPIINAGDGSNQHPTQCLLDLFTIRQTQGTLDNLDIAFVGDLKY
                     GRTIHSNVIALSHFNTTFHLVSPEELKLPSAVKMHIKSNNLQYFQYTDLEEVIPKVDI
                     IYMTRIQRERFADPIEYERVKNSYTLTKKMLEGSRENLKILHPLPRVNEISVDVDLDP
                     RAYYFKQALNGVYIRQALIASILGLA"
     gene            complement(1118..>1822)
                     /locus_tag="PK976_04950"
     CDS             complement(1118..>1822)
                     /locus_tag="PK976_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67269.1"
                     /translation="CDVILWDGGNNDFPFYKPDLMIAVADPHRPGHEVSYYPGEVVAR
                     IADIIVINKIDTACNENILTVRQNIARINPNAIVVDGASPLTVDHPELIRGRKALVVE
                     DGPTLTHGEMKIGAGTVAALKYGASELIDPRPYAIGKIADTFHIYPNIAALLPAMGYG
                     DQQIKDLEITIENTPADVVIIGTPIDLTRIIKINKPTVKIGYDLQEIGHPDLEELLVN
                     FLEKHNLTKKAPKSCC"
BASE COUNT          515 a          340 c          429 g          538 t
ORIGIN      
        1 gttccatttt gaatggcgga cacgatgagt ttttttcggc tttcgggttt catatgggtt
       61 agaataaaga taaaaatcat gattacattt tagaatcaag caaggccaag aatagaagcg
      121 atcaaagctt ggcgtatata aacaccattg agggcctgtt taaaataata ggcacgggga
      181 tcgagatcca catctacact aatctcgttg actctgggca aagggtgtag aattttcagg
      241 ttttcccttg aaccttcgag cattttttta gtaagagtat aagaattttt tactctttcg
      301 tattcgatgg ggtcggcgaa gcgctcgcgc tgtatacggg tcatgtaaat aatatccacc
      361 ttaggaatta cttcttcgag atcagtatat tgaaaatact ggaggttgtt agatttaatg
      421 tgcattttga ccgcacttgg caatttgagt tcttcgggag agacgaggtg aaaggtggta
      481 ttaaaatgag atagtgcaat gacgttggaa tggatagtac gaccatattt caggtcaccc
      541 acgaaggcga tatctagatt gtcgagagtg ccttgtgtct ggcgaatagt gaaaagatcg
      601 agaaggcatt gtgttggatg ctgattagaa ccatctccag cattgatgat ggggacactt
      661 gctacttggc tagcccatcg tgcagaacct tcaataggat ggcgcattac aatcaggtca
      721 gcataattac taaccgtgcg tatggtatcg gccaaacttt cacccttcgt ggtgctgctc
      781 aaggctgcat cgctaaaacc aatcacacgg cctcctaaac ggtttactgc actttcgaaa
      841 ctcaggcggg tacgggtact tggttcaaaa aacaaggttg ctacaacaaa accctcaaga
      901 atcttttgat taggattttt ctcgaaataa gtggcaagat caagaatttg aaggtattct
      961 tccttacaga agtcattgat cgaaatcaga cttttattct tcatgttcat agtgggtggt
     1021 tattttttcc gtttcaaatt taagttcaaa gatcaaaaaa agtaaaaatc tttctggttt
     1081 ctaaaaaaaa accccgcaat tgcggggtct tcgaatatca acagcagctt ttgggagctt
     1141 ttttggtgag gttatgtttt tcgagaaaat tcacgaggag ttcttccaga tccggatgtc
     1201 caatttcttg aaggtcgtaa ccaattttaa cagtaggttt attgattttt attatccggg
     1261 tgaggtcgat gggagtacca attataacaa catcggcagg ggtattttca atggttattt
     1321 cgagatcttt aatttgttga tccccatatc ccatagctgg gagcaaggca gcgatatttg
     1381 gataaatgtg gaaagtatcg gcaattttac caatggcata agggcgtggg tcgataagtt
     1441 cggaggcgcc atatttcaaa gcggcaaccg taccagctcc aattttcatt tcaccgtggg
     1501 ttagtgttgg gccatcttcg actaccagag cttttctgcc cctgatgagt tcagggtggt
     1561 caacggtaag cggagatgca ccatccacaa caatagcatt aggattgatg cgtgcaatat
     1621 tttgacgaac ggtgagaata ttttcgttgc aagccgtatc aatcttgttg ataacgatga
     1681 tatcggctat acgggcaaca acttcaccgg gataataaga aacttcgtgg cctggccggt
     1741 gtgggtcggc tacagcaatc ataaggtctg gcttgtaaaa agggaaatcg ttgtttccac
     1801 cgtcccaaag aataacatcg ca
//
LOCUS       DAORPD010000362         1819 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_12135_length_1819_cov_2.431406, whole genome shotgun sequence.
ACCESSION   DAORPD010000362 DAORPD010000000
VERSION     DAORPD010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12135_length_1819_cov_2.431406"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="PK976_04955"
     CDS             <1..262
                     /locus_tag="PK976_04955"
                     /inference="COORDINATES: protein motif:HMM:NF013024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uL15m family ribosomal protein"
                     /protein_id="HRC67270.1"
                     /translation="KGGFKNFNRVEYTGINLDLLQNLSDKWAITDFDLNTFIHHGLIS
                     KNERVKILGRGQISSKLNVTAHAFSKKAREVIESLGGTVTLI"
     gene            360..1712
                     /gene="secY"
                     /locus_tag="PK976_04960"
     CDS             360..1712
                     /gene="secY"
                     /locus_tag="PK976_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HRC67271.1"
                     /translation="MKKIIQTLRNIFQIEELRQRLLYTLGIILIYRLGAYITIPGIDP
                     NQLTAIRNQTKDGLLGLLNMFSGGAFSHASIFALGVMPYISASIVIQLLGIAVPYFQK
                     LQKEGESGRKKINQITRYLTIAVTALQAPGYIANLINQSPDAVLLVNPASNSPTTFFW
                     ISSVVILVAGTMFVMWLGERITDKGIGNGISLIIMIGIIARLPFALAGEFVSRMEEKG
                     GGLVFFVIEIAVLIGVILVTIMLVQGTRKIPVQYAKRIVGNKQYGGVRQYIPLKVNAA
                     GVMPIIFAQAIMFLPLTIAGFARSETLTGFAAAFTNINSFWYNFVFAILIIVFTYFYT
                     AITVNHNQMAEDMKKNGGFIPGVKPGKKTAEFIDNVMSRITLPGSIFLAFVAIMPAIV
                     RLFGVSSQFAQFYGGTSLLILVGVVLDTLQQIESHLLMRHYDGLMKSGRIKGRTSFAM
                     "
BASE COUNT          526 a          357 c          379 g          557 t
ORIGIN      
        1 aaaaggtgga ttcaaaaatt tcaatcgtgt ggaatatacc ggcatcaatc tcgaccttct
       61 acaaaacctg tcggataaat gggccatcac cgattttgat ctcaatactt tcattcacca
      121 tggactcatc agtaagaatg aaagagtaaa aatccttggt cggggccaga tttcttcaaa
      181 gctgaatgtt acagcacatg ctttttccaa aaaagcacgc gaagtcattg aatcattggg
      241 tggtacagta actttgatat aattccagat gaaagccgat cgtgaaatta tcaatagaaa
      301 ggaacatata gacaatccga aagtattcac tcagcctgaa ttaaacaatt aaattacaga
      361 tgaaaaaaat tattcagaca cttagaaaca tattccagat agaggaactt cgacaaagat
      421 tgctttatac cctgggtatc atcttgatat atcgtttggg tgcttatatt accattccgg
      481 gcatcgatcc caatcaattg actgccatta gaaaccaaac taaagatggc ttgcttggtc
      541 tgttgaatat gttttcgggt ggagcatttt cgcatgcttc aatctttgct cttggagtaa
      601 tgccatacat ctcggcttct atcgttattc agcttttggg tatcgctgtt ccttattttc
      661 agaaactgca gaaggaaggt gaaagcgggc gaaaaaaaat caatcagatt acccgctatc
      721 ttacgatagc tgttacagcc cttcaagccc ctggttatat tgccaacctc atcaatcaaa
      781 gtcctgatgc agttttgctc gtcaatcctg caagtaattc acccacgact tttttctgga
      841 tatcaagtgt cgttattttg gtagcaggta ccatgtttgt gatgtggttg ggcgagagga
      901 ttaccgataa aggcataggc aatggaattt cacttatcat tatgattggt attatagcca
      961 gattgccttt tgcgctagcc ggggaatttg tatcgagaat ggaagaaaaa ggcggcgggc
     1021 tggtgttctt cgttatcgaa atcgccgtat tgatcggtgt aatcttagtt acaattatgt
     1081 tggtacaggg aacccgtaaa attcctgtac aatatgccaa acgtatcgtt ggcaataagc
     1141 aatatggagg tgtacgtcaa tacatccctc tgaaagtgaa tgctgccggt gtaatgccca
     1201 ttatctttgc tcaggctatt atgttcctcc ccctcaccat agctggtttt gctcgttcag
     1261 aaacccttac gggttttgca gctgcattta ccaatattaa tagcttttgg tacaactttg
     1321 ttttcgcaat tctaattata gtgtttactt acttttatac agcaattacc gtaaaccata
     1381 atcagatggc tgaagacatg aagaaaaacg gtggattcat tcccggagta aaacccggta
     1441 agaaaactgc cgaattcatt gataacgtca tgtcgagaat tactttgccc ggttctatat
     1501 ttctggcttt cgttgctatt atgcctgcca tcgtcaggct attcggagta agctcgcagt
     1561 ttgcccaatt ttatggagga acttctttgc ttattctagt aggagttgtt ctggatacat
     1621 tacaacaaat cgagagccac ctgctgatga ggcactatga tggattgatg aagtcgggaa
     1681 gaataaaggg acgtacctcc tttgctatgt aaattcgagt atcattatga gtcttgtaaa
     1741 atcacccgaa gaaatagagt tggtacgtca aagttctttg cttgttggta agacccttgc
     1801 ggaagttggc aaacatatc
//
LOCUS       DAORPD010000363         1819 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_12138_length_1819_cov_2.065193, whole genome shotgun sequence.
ACCESSION   DAORPD010000363 DAORPD010000000
VERSION     DAORPD010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12138_length_1819_cov_2.065193"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..812
                     /locus_tag="PK976_04965"
     CDS             <1..812
                     /locus_tag="PK976_04965"
                     /inference="COORDINATES: protein motif:HMM:NF014799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRC67272.1"
                     /translation="FSMGIAEQNGMGVAAGLALEGKIPVFSTYAVFSAFRAADQLRIS
                     VCYNNLHVIIGGAHAGISVGPDGATHQALEDLAYVRALPNITVLSPCDASQAFKATLS
                     ALLEIAGPVYIRFGRENVPDFTTYDLEFIPGKAQVFRPGKDVTIIATGHLVWPSIEAS
                     ELLAKQGISARVINLHTIKPIDEETIIRAAAETGCILTAEEHQIHGGMGSAVAEVIVR
                     DHAVPMEMIGINDTFGESGQPDELMKKFGLTAENIAQKTTQLLKRKKITNS"
     gene            812..1423
                     /locus_tag="PK976_04970"
     CDS             812..1423
                     /locus_tag="PK976_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67273.1"
                     /translation="MNTFIVLLQSTIPSLLVLAVAVYFFHASNEQFLKRWKDTVPLLK
                     DDRTERLLKSFIEREEKLQLLSIKKENQRITLPLRLQAYERFVLLLERITLSRLLLRV
                     AEPALTAQQLQHSLILNIRNEFEHNLSQQIYISETSWEWIKKTVEESIQLINTSASTI
                     PADSSASVLISLLMEMENSGQAPQTHKVISHLHAEAQFLLAWG"
BASE COUNT          533 a          346 c          411 g          529 t
ORIGIN      
        1 ttttttctat gggtattgcc gaacaaaatg gcatgggagt agctgcagga ttggccctcg
       61 aagggaagat tccggtattt tcgacttatg ctgtattttc tgcttttagg gcagctgatc
      121 agctcagaat ttcggtttgt tacaataatt tacatgtaat aataggtggg gcacatgccg
      181 ggatatctgt gggacctgat ggagctacac atcaagcgct cgaagacctt gcttatgttc
      241 gcgctttgcc gaatataacg gttctgagtc cctgtgatgc tagccaggct ttcaaggcaa
      301 ctctatcggc tttattggag atagcagggc ctgtttatat acgatttgga agggaaaatg
      361 tacccgattt tacaacttac gatctggaat ttatcccagg gaaggctcag gtttttcgtc
      421 cgggaaagga tgttaccatc attgctactg gccatttggt atggccgtca atcgaagctt
      481 cagaattgct tgctaaacag gggatttcag ccagagtcat caatctgcat actattaaac
      541 cgatcgatga agaaaccatc atcagggcgg ccgcagaaac ggggtgcata ctcactgccg
      601 aagagcatca gatacatgga ggaatgggta gtgctgtggc tgaagtgatc gtgagagatc
      661 atgccgtacc tatggaaatg atcggaatta atgatacttt cggagaatcg ggacaacccg
      721 atgaactgat gaaaaaattc ggcctgactg cggaaaatat tgctcaaaaa acgactcaat
      781 tattgaaaag aaagaaaata acaaactcat aatgaataca ttcatagttt tattacaaag
      841 cactatccct tcattactgg tgttggccgt agcggtttac tttttccatg cttctaacga
      901 acaatttctg aaaaggtgga aagacacagt acctctctta aaggatgacc gaacggaaag
      961 attgttgaaa tcattcatcg agagagaaga gaaattacag cttttgtcca tcaaaaaaga
     1021 aaatcagcgt attacacttc ctttacgatt acaggcttac gagcgttttg tgctcttgct
     1081 cgaacgtatt acgttatcac gcttgctact gagagttgca gaacctgcat tgactgccca
     1141 acagcttcaa cattccctta ttctgaacat tagaaatgaa tttgagcata acctttcgca
     1201 acagatttat atttcggaaa catcgtggga atggatcaaa aaaacggtag aagaaagtat
     1261 tcaattgatc aatacttcgg cttcgacaat acctgccgat tcatcggctt cggttttgat
     1321 ttcgctgctt atggagatgg aaaattccgg ccaggcacct cagactcata aagtcatcag
     1381 ccacctacat gccgaagctc agttcctttt agcctgggga tagattttta tatcacagga
     1441 tttgatgacc ataccactga tatctaccat agactttagt gtgataattt aaatcggtaa
     1501 aagaagcggg tagaattatc agagttggga tcattatcaa cgcactccat attttgtaaa
     1561 atctcgaatt ttcttagcca ttgatgatgg tgttttactc gaacagaatc cgaatggata
     1621 agctcgacca attgagtatc agaatgctta tcatcttgca atgattgtgt aatgaagaca
     1681 tttttactgg tacatttccg gattggcttt atggtagctt ggcataaacc ttagttttgt
     1741 tcataagaat tgggagtttt aactctgaga gttcgggggg aaaaggtttc gggaaaggct
     1801 atgtgcgata tgattgagc
//
LOCUS       DAORPD010000364         1818 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_12147_length_1818_cov_2.464549, whole genome shotgun sequence.
ACCESSION   DAORPD010000364 DAORPD010000000
VERSION     DAORPD010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12147_length_1818_cov_2.464549"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(401..>1818)
                     /locus_tag="PK976_04975"
     CDS             complement(401..>1818)
                     /locus_tag="PK976_04975"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HRC67274.1"
                     /translation="VAGLKDKWVFTLAKPSMIPFLQYSERRDLREKIYRAYFMRGDNN
                     NEYDNKKIIADMVKLRAEKAELLGYDNWASYVIAENMAKTPIAVDEFLMNLWNAALPV
                     AKNEASQMQAIIHKEGGNFELEPWDWWYYAEKICREKYNIDENEIKPYFSLQNVRQGM
                     FDVANKLYGITLSRRTDLPMYHKDVEAYEVKDADGSHLGILYLDYYPRAEKRVGAWCT
                     EFRSAGWENGQRVSPVVSIVYNITPSTASTPSLLNWDETTTLFHEFGHALHALFAEGK
                     YRRTAGVVPRDFVELPSQIMENWAQEPEVLKIYAKHYQTGEIIPNTLVQKIEKSGQFN
                     QGFETVEYLAAAILDMKYHELASQNANIEPNTFEKKSMDEIGLIKEIIPRYRSTYFQH
                     IFSGGYSAGYYVYIWAEVLDADAFDAFVKSGDVFNKELASKFRNYCLAHVGEDDAMTQ
                     YVKFRGQQPSIGPLLKRRGLK"
BASE COUNT          502 a          382 c          351 g          583 t
ORIGIN      
        1 atcggtcagg tagtttttct ggtcttcaga tgatacagca ataaaagcct gatctttccc
       61 atccatcctc catcttaata ataaaataaa tctaagttag taatcgtcgg gcataattaa
      121 tctctcctct gacttatggt gttttatacg caaggtaaaa taatgtagtt atttgaagaa
      181 atgggcatca ctgtcttgtt ctgacaataa tattaaaatc agcttctgaa aatttataat
      241 tcaattgaaa tacaaggttc tatatttatc cattgccgct tttctgtctc tttcgtatgt
      301 gggatagatc tgataagaat agaagttgct tcgaaaaaat aaaaaaaacc cgtaatgatt
      361 tacgggtttc aataaggcta aataaatatg gaattgattt ttattttaaa cctcttcttt
      421 tcagtaaagg cccgatggaa ggttgctgcc cgcggaattt cacatactgt gtcatggcgt
      481 cgtcttcgcc aacatgcgcc agacaataat tgcggaactt cgaagccaat tccttattga
      541 aaacatctcc gcttttgacg aatgcatcga aagcatcggc atccagaact tcagcccaga
      601 tatacacata ataaccagca gaatagccac cgctaaagat atgttgaaaa taagtgcttc
      661 tgtatcgggg gattatttcc ttgattaaac cgatttcgtc cattgatttc ttttcgaaag
      721 tattaggctc tatgttggcg ttttgagatg ccaattcatg atatttcatg tcgagtatag
      781 ctgctgcaag atattctacg gtttcaaaac cctggttaaa ttggccgctt ttttcgattt
      841 tctggacgag tgtgttaggg attatttccc cagtttgata atgttttgca tatattttca
      901 gtacttcagg ttcctgagcc caattttcca tgatctgaga agggagttcg acaaagtcgc
      961 gaggaactac tccggcagtt cgtcggtatt tcccttcagc gaaaagtgca tgcaatgcat
     1021 gtccaaattc atggaaaagc gtggtagttt catcccagtt gagtaatgat ggggtggagg
     1081 ctgtggaagg agtaatgttg taaacgatgg aaaccacggg acttactctc tgtccgtttt
     1141 cccagcctgc cgaacgaaat tccgtacacc atgcgcctac gcgtttttca gcccttggat
     1201 aataatccaa ataaagaata cccaaatgac tgccatcggc atctttcact tcataggcct
     1261 caacgtcctt atgatacatt ggcaggtccg ttctgcggga aagagtaatc ccataaagtt
     1321 tattggctac atcgaacatt ccctgacgca cattttgaag tgaaaaataa ggttttatct
     1381 cattttcatc tatattgtat ttctctctgc atattttttc ggcataatac caccaatccc
     1441 acggctcaag ttcaaagttt cctccttcct tgtggatgat cgcttgcatt tgtgaggctt
     1501 cgttcttggc cacgggtaga gcggcattcc atagattcat taggaattca tctacagcaa
     1561 tgggagtttt ggccatgttt tcggcaatta cataagatgc ccaattatca taccccagca
     1621 gctcggcttt ttcagcccgg agcttcacca tatcagcaat aatttttttg ttatcgtatt
     1681 cattattatt atcaccccgc ataaagtagg cacgataaat tttctccctc agatccctac
     1741 gttctgaata ttgcagaaaa ggtatcatgc tgggtttggc aagggtaaat acccatttgt
     1801 ccttcagccc tgcaactt
//
LOCUS       DAORPD010000365         1816 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_12164_length_1816_cov_2.379898, whole genome shotgun sequence.
ACCESSION   DAORPD010000365 DAORPD010000000
VERSION     DAORPD010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12164_length_1816_cov_2.379898"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            239..>1816
                     /locus_tag="PK976_04980"
     CDS             239..>1816
                     /locus_tag="PK976_04980"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRC67275.1"
                     /translation="MLLLIFEPLQADDVRGMFFYHQRLDDLRQNITKAKNPEEKIRNY
                     LLLSKMYQIISADKASAYADSAIFLSHSAELHSLEADALLLKARVYMIGYVTDSANLF
                     LDRAYNKALKCKAYSSLGYILTTQAIKSMYENDKGVFLDEINQAEKYFKLAEDYEGLV
                     YAYNTRASLYISLNLYEEARSYIHSAMEEADKLNDQSFKIPIQLKQADLLSSTGNHQQ
                     AISLILLNIPYLKKNNLVYLEISSLRSISSLYIKLGEYNKALEYAYDAYDKCKPLGFH
                     LLASDLLTLIGNIFHRTNNYDLSIRMNHQARTLRYYNHFPLACFNSYYNLGALFGAIN
                     QIDSSLYWLNQAMALANKYNNIETKALTAELFYQTYESAGLYEPALNYFQLWSEYKAQ
                     RNQTVTKNAFRALQATLDVQRQKEQYELNTKRIQTRLIFSYLLAGLTLLVIIIALLLI
                     RSYKNKIKVQYSQMRERLLRMQMNPHFIFNALIAIQSYIYKNDTSKAIGFLDDFSNLI
                     SLFLTSSSKDFIPLSEEL"
BASE COUNT          563 a          360 c          309 g          584 t
ORIGIN      
        1 atgaaacttc ggttttgtcc ctccttcttt ttatccaatt tttttgtcgg tcagtaatga
       61 ttaaatagat aacatccatg ctatcaataa atctttatca acgatatttg taattctata
      121 aaaattctat ctttgaaagg tttactccaa aattggttct cgtatgattc atttcatgag
      181 aaaatgctat attaatgcat tcaatgtgaa aagaattttt catttcttta ggattttgat
      241 gctactgctt atatttgagc ctcttcaggc cgacgatgta agaggcatgt ttttctatca
      301 tcaacgactc gatgacttaa gacaaaatat tactaaagcg aaaaatcctg aggagaaaat
      361 cagaaattat ctgctgcttt cgaagatgta ccagataatt tcggccgata aagcttctgc
      421 ttacgccgat tcggcaattt ttttgagcca ttctgcagaa ttgcattccc ttgaagccga
      481 tgctctcttg ttgaaggcca gagtttacat gattgggtat gtgaccgatt cagctaactt
      541 atttctcgat agggcataca ataaagcttt gaaatgcaag gcttattcat ccctgggata
      601 tattttgact acccaagcca ttaaaagtat gtacgaaaat gataaaggtg tatttctcga
      661 cgaaatcaac caggctgaaa aatatttcaa acttgccgaa gattacgaag gccttgtata
      721 cgcttataat acaagagcaa gtttatatat tagtttgaat ttgtatgaag aggcccgtag
      781 ttacattcat tctgcaatgg aagaagcaga caaattgaat gatcaatcgt ttaaaattcc
      841 cattcagctc aaacaagccg atttactttc ttcgacgggt aatcatcagc aagccattag
      901 tcttatctta ttgaatattc cctatctcaa gaaaaataac ctcgtttatt tagaaatcag
      961 cagtttacgc tcaatttcct ctctttacat aaaattagga gaatataata aggctttgga
     1021 atatgcctat gatgcttacg acaaatgtaa gccattgggc tttcatctcc tggcttcaga
     1081 tcttctcact ttaataggga acatctttca tcggacaaac aactatgatc tcagtatacg
     1141 aatgaatcat caggcaagaa cgttgcgtta ctacaatcat ttcccgctgg cctgtttcaa
     1201 ttcctattat aatctgggtg ctttattcgg ggctataaat caaatagata gttctcttta
     1261 ctggttgaat caggccatgg ccttggctaa taaatacaac aacatagaaa cgaaggcttt
     1321 gacagccgaa ttgttctatc aaacttatga aagtgccggg ctttacgaac ctgccttaaa
     1381 ttattttcaa ctctggtcgg aatataaagc ccaacgaaac caaacagtaa ccaaaaatgc
     1441 gtttcgtgcc ctgcaagcca cactcgatgt ccaaaggcaa aaggagcaat acgaactgaa
     1501 caccaaacga atacaaactc ggcttatctt ttcatatctt ctggctggcc ttacgctcct
     1561 ggttatcatc attgccttac ttcttatacg ttcatataag aataaaatca aagtgcagta
     1621 tagccagatg cgtgaaagat tactccgcat gcagatgaac cctcatttca tcttcaatgc
     1681 tttaatagcc atacagagct atatctataa aaatgatact tcgaaggcca ttggttttct
     1741 cgatgatttt tcgaatctta tcagtctgtt tttgacttct tcgtcgaaag attttatccc
     1801 cttgtcggaa gaattg
//
LOCUS       DAORPD010000366         1816 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_12165_length_1816_cov_2.312890, whole genome shotgun sequence.
ACCESSION   DAORPD010000366 DAORPD010000000
VERSION     DAORPD010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12165_length_1816_cov_2.312890"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1250
                     /gene="gatD"
                     /locus_tag="PK976_04985"
     CDS             <1..1250
                     /gene="gatD"
                     /locus_tag="PK976_04985"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatD"
                     /protein_id="HRC67276.1"
                     /translation="NDDDLHIVIKIATGYNIGIDVRTIIEMKEIGYKKANYKIPEKQF
                     PFTPGLPKVKLLGTGGTIASRLDYRTGAVIPAFTPGELYGAVPELADICNLETEKLFA
                     VFSENMGPTQYKALAIAIGKEIEKGVDGIVIGHGTDTLSHTGAALSFMVQNSPVPIIL
                     VGSQRSSDRPSSDAALNLMHAMTAAGHGDIAEVLVCMFGPTSDEYGLLHRGTRVRKMH
                     SSYRSTFRTISDTPVAMVTRKGVTPIKKKYNPRRKDRQVIIKPYYDERVTMLYYYPGM
                     HPDILNGIIDMGYKGIIFVGTGLGHVNKELYPAIERAHKLGIHMFMTVQTIWGYVHMF
                     VYDTGRDMMAKGIIPAGNMLPETAYIKLSWVLGQTDDPEEVKRLMLTPINDEITEREP
                     YNGYLVFQGGVPEIEQFLKRVHK"
     gene            complement(1554..1625)
                     /locus_tag="PK976_04990"
     tRNA            complement(1554..1625)
                     /locus_tag="PK976_04990"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1590..1592),aa:Glu,seq:ttc)
BASE COUNT          590 a          414 c          328 g          484 t
ORIGIN      
        1 aaaacgacga tgatttacac atcgtcataa agatagccac cggctacaat attgggatag
       61 atgttcgtac cattatcgaa atgaaagaga taggttataa aaaagccaat tacaagatac
      121 ccgagaagca attccctttt acacctggtc tccccaaggt aaaacttttg ggtactggcg
      181 gtacaattgc atcaaggctc gattatcgga ctggagcggt tataccggct tttacgcctg
      241 gtgaacttta cggagctgtg cctgaattag ctgacatttg taatctcgaa accgaaaagc
      301 tttttgctgt attcagtgag aatatgggcc ctacccaata caaagcgctt gctatagcta
      361 taggaaaaga aatcgagaaa ggagtagatg gcattgtgat agggcatggc acagacacgc
      421 tttcacatac aggagccgca ctttcgttca tggtacagaa ttctcctgta ccaatcatct
      481 tggttggttc gcagcgttca tccgacagac caagcagtga tgccgcattg aacttgatgc
      541 atgccatgac tgccgctggc catggcgaca tagccgaagt tctagtttgt atgtttggac
      601 ctaccagcga tgaatatggc ttacttcatc gtggaaccag agttagaaaa atgcactcga
      661 gctaccgctc cactttccgc acgattagcg acactcccgt agctatggtt acacgtaaag
      721 gcgtcactcc catcaagaaa aaatataatc ctcgtagaaa agatcgccaa gtaattatca
      781 aaccctatta tgacgaaagg gttaccatgc tctattacta ccctggcatg catcccgaca
      841 tactcaacgg aatcattgat atgggctata aaggaattat atttgtagga actggcctgg
      901 gacatgtaaa caaggaactt tatcccgcca tcgaaagagc acataaattg ggtattcaca
      961 tgtttatgac cgttcaaact atatggggct atgttcacat gttcgtttac gatactggac
     1021 gcgatatgat ggccaaagga atcattccag caggcaatat gctacccgaa actgcttaca
     1081 tcaaactgag ctgggtttta ggtcaaaccg acgatcccga agaagtaaaa cgtcttatgc
     1141 ttactcctat caatgatgaa attaccgaac gagaacctta taatggctat cttgtttttc
     1201 aaggtggcgt acccgagata gagcaatttt taaaaagagt gcataaataa aaattccctt
     1261 cgaataaatt cgaatgaaga tattcaaaca actgacaaca aacaactatc aaactccaaa
     1321 ttacaaatta caaacaaatt ccaaaatcca actaacaatt acaaaattcc aaattctaat
     1381 caccatccac tatccaccaa ccaccttgtc tcttcgtctc ccagtcctca ttcaccaatc
     1441 actatccacc attctccgtt cacctaaatt aaaacaaaaa cactcaacac cattgaagca
     1501 tcgacaacag aaaacatcaa ttatttcaga tttctctctt caccttcatt ttgtagcccg
     1561 taggggaatc gaacccctgt tacaaggatg aaaaccttgc gtcctaaccc ctagacgaac
     1621 gggccttata tgaaagaact tacttgtttt tcgggctgca aaattagagg ttttatacga
     1681 atcccacaat attcggctaa taattttcaa aatattacga aacacgcctg aaaactaatt
     1741 cattagaaat tcatccccat tttttcgggg gaatggatca ttccaaacaa tttattgata
     1801 atgaatattt tatatt
//
LOCUS       DAORPD010000367         1815 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_12172_length_1815_cov_2.495455, whole genome shotgun sequence.
ACCESSION   DAORPD010000367 DAORPD010000000
VERSION     DAORPD010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12172_length_1815_cov_2.495455"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..942
                     /locus_tag="PK976_04995"
     CDS             100..942
                     /locus_tag="PK976_04995"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-archaeol synthase"
                     /protein_id="HRC67277.1"
                     /translation="MKYKTLLVRSATGALYVAMVVFSIALGPWWFAALMFIFFLLSLL
                     EYLHLVLPSKEKILKCLTIIVSSGLYCILCFTALNPLLQTEKLLAWSILLIPILLTFS
                     LFIKSSDILMKIALVLLGIIYITFPFVLLNFIGNPWLTKFDDPRIILLSFFIFVWTND
                     TFAYLMGVNFGRHRLFERLSPAKTWEGMLGGIFFTLVMGILLSCFFKELSFIQWIGMA
                     LITSVSGILGDLSESLIKRTFGVKDSGKAIPGHGGFLDRFDSILFAAPSIFVYFIMIS
                     LITL"
     gene            982..1650
                     /locus_tag="PK976_05000"
     CDS             982..1650
                     /locus_tag="PK976_05000"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF003678.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HRC67278.1"
                     /translation="MSKIHPEALKIVPPIALVLLIISGVLTFIFRDSLTGIIIVWVLA
                     LVFIFLFTLFFRFPKRPRPQNGRKVYSSADGKVVVIEEVMEKEYFGEKRIQLSVFMSI
                     TNVHINWSPVTGKVIYEKYKPGRFFPAWMPKSSALNEHNTFVVETHSGHQIMVRQIAG
                     AVARRIAVYKRCGDEVKAGDQLGFIRFGSRVDLFLPLGSEIKVNIGQHVQGSRTLLAI
                     LPPA"
BASE COUNT          490 a          347 c          359 g          619 t
ORIGIN      
        1 gtgtgatcaa caaacaggat tctgaactca ttataggttt tgccgaagtt tatgtaaaac
       61 gagaagataa agaaaaagct ctgcaaatca ttcgatcacg tgaaatataa aactttattg
      121 gtcaggtcgg ctaccggagc attgtatgta gccatggtag tgttttccat cgctttggga
      181 ccctggtggt ttgcagccct gatgttcatt ttctttttat tatccctgct tgaatatctt
      241 catctggttt taccatcgaa ggagaagatt ctgaaatgct tgacgattat agttagcagt
      301 ggactttact gcattctctg cttcacagcc ttgaatcctc ttttgcaaac agaaaagtta
      361 cttgcctggt cgatcctatt gatacctatt ttgttgacct tttcactctt tattaaaagc
      421 tctgatattc tgatgaagat tgctctggtc ttattaggta tcatctatat tacctttcct
      481 tttgtgttgc ttaatttcat tgggaatcca tggcttacca aattcgatga tcctcggatt
      541 atacttttga gtttttttat tttcgtttgg acgaatgata cctttgctta cttgatggga
      601 gtaaattttg ggcgtcatcg tctcttcgaa aggctcagcc ccgcaaaaac atgggaagga
      661 atgttgggag gaattttttt tactcttgtt atgggcatcc tgttatcctg tttcttcaaa
      721 gaactttcgt ttatacaatg gataggaatg gctctgatta cttccgtttc tgggatactt
      781 ggcgatttat ctgagagctt gataaaacgg actttcggtg tcaaagatag tggtaaagca
      841 atacctggtc atggaggttt tctcgatcgt ttcgattcca tcctgtttgc tgctccatct
      901 atttttgttt attttatcat gatatcgctc attacgttat aattttgcaa tcgtaaaatc
      961 ttattatacc gaaaacaagg aatgtcaaaa attcatcctg aagcattgaa aatagttcca
     1021 cctatagcct tggtattgct gattatttcg ggagtgctta ctttcatttt ccgagatagt
     1081 ttaacgggaa ttatcatcgt ttgggtattg gctcttgttt ttatctttct ttttactcta
     1141 ttttttaggt ttcccaaaag gcctcgaccg caaaatggga gaaaggtcta cagttcggcc
     1201 gatggaaaag ttgtcgtgat cgaagaagta atggaaaaag aatattttgg tgagaaacga
     1261 atccaattat ctgtctttat gtcgattacc aatgtacata tcaattggag ccctgtaact
     1321 ggcaaggtta tctatgaaaa atataaaccc ggaagattct tccctgcttg gatgccaaaa
     1381 tcttcggcat tgaatgagca caataccttt gtagtagaga ctcattctgg acatcaaatc
     1441 atggtgcgtc aaattgcagg agcagttgcc cgacgcatag ctgtttacaa acgttgtggc
     1501 gatgaggtca aagccggcga tcagttgggc ttcatccgct ttggctcccg agtcgaccta
     1561 tttttgcccc tgggttccga aataaaagtg aatatcggac agcatgtaca aggcagccga
     1621 acgcttctgg ccattttgcc accagcatga atcaccttct tcagaaactt tctaccttaa
     1681 ttcccaatca gattgcaaat tataattctc aataggatat cattcaaaac taatgtgcaa
     1741 tgaggcagct actggaattc aattcatcaa atgatatgaa acacaaaaat cgtatttctt
     1801 catcatatat aatcc
//
LOCUS       DAORPD010000368         1814 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_12187_length_1814_cov_2.217169, whole genome shotgun sequence.
ACCESSION   DAORPD010000368 DAORPD010000000
VERSION     DAORPD010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12187_length_1814_cov_2.217169"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..542)
                     /locus_tag="PK976_05005"
     CDS             complement(36..542)
                     /locus_tag="PK976_05005"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRC67279.1"
                     /translation="MSEIEFNYQLLSLQNSLNSFALSLTKNVDEASDLVQDTYLKAII
                     YRDKFDAGTNLKAWTYTIMKNTFINNYRREVRLNAFIDSTDDIYHLNAKSTMEAERPD
                     SSLHAKEIQKSIQSLGEEHRIPFLMHTRGYKYKEIADQMNLSIGTVKSRIFFGRKKLM
                     EILRDYQN"
     gene            complement(783..1685)
                     /locus_tag="PK976_05010"
     CDS             complement(783..1685)
                     /locus_tag="PK976_05010"
                     /inference="COORDINATES: protein motif:HMM:NF014646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRC67280.1"
                     /translation="MSKYLIKDIERFTGIKAHTLRVWEKRYHVLKPCRTSTNIRTYDD
                     DDLKRILNISILNRHGYRISEIIKLSEEEICEKVMQLGQRCADTHSQIEKLIMAMVQM
                     DEEIFQEVFDQSVKKSGFEDTFYYLIFPFLERTGLLWQSGSILPAHEHFISNLIRQKL
                     IVAIDSILSPRKPNALHSLLFLPEGEWHEIGLLFTYYLIKKWGHYTLYLGPHLPIENI
                     ESTLLKHSFDFLVTQFINGISGNDLNLQIQKIVKICKGKKVLLSGGRLIETPFDLPPD
                     FFIYENTQQLKEIIDNQYILKIKS"
BASE COUNT          550 a          304 c          348 g          612 t
ORIGIN      
        1 atatatgcgc catatattct aactcgttgg aaaatttaat tttgataatc gcgtagaatt
       61 tccatcagtt tctttctgcc gaaaaaaatt ctactcttta cagtacctat cgaaaggttc
      121 atttgatcgg caatttcctt atacttatat ccacgcgtat gcatcaagaa tgggatgcga
      181 tgttcttcgc cgaggctttg aatacttttc tgtatctcct ttgcgtgtag gctcgaatct
      241 ggcctttcgg cttccatggt gctctttgca ttcaaatgat aaatatcatc agtgctatcg
      301 ataaaggcat tcaagcgaac ctcccgacgg taattgttaa tgaaggtgtt tttcatgatg
      361 gtgtaagtcc aagctttgag atttgtccct gcatcaaact tgtcgcggta gattatcgcc
      421 ttcaaatagg tatcctgtac cagatcgctt gcctcatcaa cattcttagt caatgataaa
      481 gcaaaactat tcaggctgtt ttgtaaactt aacaattgat aattaaactc tatttcgctc
      541 ataatgtgga atttttacta attagaaaat ttctaaagtg tttaaatttt atggcacaaa
      601 tttaaacaat atttctaaaa tatgttaaca ctttcctata taaattttta aatatttatt
      661 caaaaaatgc cattacattg atttacaata taaattggtt gtataattta acatttatta
      721 acttttcttg cttaaaaaag aaaagccaat gtaatttatc aatggagaag ggagaggaag
      781 gattagctct ttattttcag tatatactga ttatcaatga tttccttcaa ttgttgggtg
      841 ttttcgtaaa taaaaaaatc gggaggaagg tcgaaaggcg tttcgataag tcgaccgccg
      901 cttagaagaa cttttttgcc cttgcatatt ttgactattt tctgaatctg aaggtttaaa
      961 tcgttgccag aaattccatt gataaattgg gtgaccaaga agtcgaaaga gtgtttgagt
     1021 aaggttgatt cgatattttc gatgggaaga tgaggcccga gataaagtgt ataatgtccc
     1081 cattttttta tcagataata agtaaacaac aatcctattt catgccattc cccttcggga
     1141 agaaaaagca atgaatgcaa ggcattaggt tttcgcgggg agaggataga atcgatggct
     1201 acgattaatt tttgcctaat caggttactg atgaaatgtt catgagcagg cagtatgctt
     1261 cccgattgcc agaggaggcc ggttcgctct aaaaagggga atatgaggta gtaaaaggta
     1321 tcttcgaaac cacttttttt aaccgattga tcgaaaacct cttgaaaaat ttcctcatcc
     1381 atttggacca ttgccatgat gagtttttcg atctggctat gcgtatcggc acatcgctga
     1441 ccgagctgca ttaccttttc gcaaatttct tcttcagaaa gtttgatgat ctcagatatt
     1501 ctatagccat gccggttaag gatagagata ttgagaatac gttttaaatc gtcgtcgtcg
     1561 taagtacgaa tattggtcga agtacggcag ggtttcagga cgtgataacg cttttcccag
     1621 actctgaggg tgtgagcttt aattcccgta aatctttcaa tatctttaat caaatatttt
     1681 gacatggtta aacttttgaa ataagaaagc tgtcgagcaa cagtctatcg ttattttaac
     1741 aattggaatg attatatgtt taataagaaa tttcgaaggg ttgaaaatgt tcgatgaaat
     1801 aacatattgg cctg
//
LOCUS       DAORPD010000369         1807 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_12263_length_1807_cov_2.228311, whole genome shotgun sequence.
ACCESSION   DAORPD010000369 DAORPD010000000
VERSION     DAORPD010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12263_length_1807_cov_2.228311"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            69..1523
                     /locus_tag="PK976_05015"
     CDS             69..1523
                     /locus_tag="PK976_05015"
                     /inference="COORDINATES: protein motif:HMM:NF014930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67281.1"
                     /translation="MRLIQLSVEEDACREISDIGHFRTFKTDRLGMPLIETVTYPDCT
                     NPDEVMEACNYIRFLNRSTGHVRTGSGAGREDVNVSCRGGHRVEIKGVSHTRWIPELT
                     HNESFRQWALLRIRDKLQSRIQKENWKMTYKRLNFFDFHFDLPALILARDNRYRIIAV
                     NLSQFRGILSHFTQPEHCFANEISDRLKVIACIEKPNMTHSESFTPEINEEDWSKICQ
                     ILQAEENDAQIVFWGPEDDISTALETIEERCWMAFDGVPPETRKSFEDGTTIFERVLP
                     GADRMYPDTDSAPIPLTDEYIEEIQKDLPTEVMVRYNQLVQWKVPQDTFTYIFKKNLY
                     PLIERIYTELDVPARFTGSFLGHTVKYIEGQKGPSSTFQFDRIYDLFEYLKNNHLDLR
                     LAQKMFPEVYDHPGMDFDSVLTILKFKRVSKEDLLSKINFLSKKFDEIAINPQPENKI
                     NWIMGQLQETALGNISMFELRNLLESVVQLPEKE"
BASE COUNT          579 a          369 c          357 g          502 t
ORIGIN      
        1 tacccacagg atttcagcgc acggccattg tgggagtgga aggcgaaatt ccttgacggc
       61 acaagaaaat caggcttata caactgagtg tcgaagaaga cgcctgccgt gaaatttccg
      121 atattggcca ttttcgtact ttcaaaaccg atcgtttggg aatgcccctg atagaaacgg
      181 taacctatcc cgattgcacc aatcccgatg aagttatgga ggcctgcaat tatattcgat
      241 ttctcaaccg tagcacggga catgtaagaa cgggcagtgg cgcgggacgt gaagatgtaa
      301 atgtaagctg cagaggaggt catcgtgtag aaatcaaagg agtttcgcat acccgctgga
      361 ttccagaatt aacgcataat gaatcttttc gtcaatgggc attgttgcgc attcgagata
      421 aacttcaatc tcgtatccaa aaggaaaact ggaaaatgac ttataaacgt ctgaattttt
      481 tcgatttcca tttcgatttg cccgccttga ttttggcccg cgacaatcgt tatcggatta
      541 ttgcagttaa cctttctcaa tttcgcggca tcctttctca tttcactcaa ccagagcatt
      601 gctttgccaa cgaaatttct gaccgactta aggtaattgc ctgcatagaa aagcccaata
      661 tgacgcattc tgagtccttt acaccagaaa ttaatgaaga agactggagc aaaatctgcc
      721 agatattgca agctgaagaa aatgacgctc agattgtttt ttgggggccc gaagatgata
      781 tctctaccgc attggaaact attgaagaac gctgttggat ggcattcgac ggagttccac
      841 ccgaaacccg gaaatccttc gaagacggta ctactatatt tgaacgcgtt ttacccggcg
      901 ccgaccgtat gtaccccgat accgattctg cacctatacc tctgaccgat gagtatatag
      961 aagaaattca aaaagatttg cccactgaag tcatggttcg ttataaccaa ttggttcaat
     1021 ggaaagtacc tcaagataca tttacttaca tcttcaagaa gaatctttat cctctcatcg
     1081 aacgcatcta taccgagctt gatgttccag cccggtttac aggaagtttt ctgggacata
     1141 ccgtcaaata tatcgaggga caaaagggtc cgtcatccac attccaattc gaccgcatct
     1201 acgacctctt cgaatacctg aagaataatc atctcgattt gagactggcg caaaaaatgt
     1261 ttcccgaagt ttacgaccac cctggaatgg attttgactc agtacttacg attcttaaat
     1321 ttaaacgtgt atcgaaagaa gatctccttt ccaaaataaa tttcttatcg aaaaaattcg
     1381 acgaaatcgc catcaaccct caacctgaaa ataaaattaa ctggatcatg ggacaacttc
     1441 aggaaactgc tttgggcaat atttccatgt ttgaattgag aaatttactc gaatccgttg
     1501 ttcaattgcc tgaaaaggag taaaacatga agaactattc atgtattttg ctaaattcta
     1561 agattattaa agatatatat aaacagttta aaaatgctaa tttagaaata aaaaaaataa
     1621 tattcgattt tttatttttt gaaataattg aaaaatggaa aagctgtcaa taataatcgg
     1681 atcaactaaa tttaattact tacaatgagt gaagatattt ttcagggcta taaaggcagg
     1741 gcactcgaaa tattgaagca attcaacgtt agagtatggg gacaggctga aataaaaacc
     1801 agtcggg
//
LOCUS       DAORPD010000370         1805 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_12281_length_1805_cov_2.294286, whole genome shotgun sequence.
ACCESSION   DAORPD010000370 DAORPD010000000
VERSION     DAORPD010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12281_length_1805_cov_2.294286"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1521)
                     /locus_tag="PK976_05020"
     CDS             complement(<1..1521)
                     /locus_tag="PK976_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HRC67282.1"
                     /translation="MFKEYNQQQLSLPMDLESMIPTNHMVRVINSAIDQMNLDPLLAK
                     YPGGGRASFHPVMMTKLIVYAYADKIYSSRRIEKAARENIMYMWLCGGNAPDFKTINS
                     FRGERMKDVILDIFSEVVELLIKQGYIKLENYFLDGTKIEANANKYSWVWGKSTKRYK
                     EKLREKCKELFAMAELINDEENMEYGDRNLEELGEKPIDAKAIEEAVQRIDERLAANP
                     ADKTLKKAKKLIEKDYLPRMQKYEKQEELLAERNSYSKTDTDATFMRMKEDHMKNGQL
                     KPGYNVQIGTENQFVVGYSIHQFAGDTSCMKDHLEEVKKILGGKLPANIVADAGYGSE
                     ENYEYLENESLGNYVKYNTFHKEATRKWKSDITRVQNWQYNEEVDEYICGNGQTLRFK
                     YETTQKSKNGYSSLIRVYESDDCKDCPYREYCIKQTDKPDFNKKIYINRRGNELKAKA
                     RDNLISDYGKQMRSLRPVEVESVFGNIKGNSGMRRFILKGLNKVKLEWGLLCIAHNM"
BASE COUNT          416 a          391 c          310 g          688 t
ORIGIN      
        1 catattatgg gcgatacata gcaaccccca ttccagcttt actttattta atcctttgag
       61 aataaatcgt cgcataccag agttgccttt gatattacca aagacacttt ctacctctac
      121 aggtcttaaa ctgcgcatct gcttcccata atcactaatt agattatctc gtgctttagc
      181 ttttagctcg ttgcctcgcc tgttgatgta gatttttttg ttgaaatctg gtttatcagt
      241 ctgttttatg cagtactcac gatagggaca atccttacaa tcatcacttt catagactcg
      301 gattaaactg ctgtatccat ttttgctttt ttgggttgtc tcatatttaa atcgcagagt
      361 ttggccattg ccgcaaatgt attcatctac ttcttcgtta tattgccaat tctgtacccg
      421 agttatatct gatttccatt tgcgagttgc ttctttgtga aacgtgttgt attttacata
      481 gttcccaagg gattcgtttt ctaggtattc atagttttct tcactgccat atccggcatc
      541 agctacgatg ttggcaggaa gcttcccccc aagaattttc tttacttctt ccaggtgatc
      601 tttcatgcaa ctggtatctc cagcgaattg gtgaatacta taaccaacca cgaattggtt
      661 ctcggtacct atttgtacgt tatacccagg ttttaattgc ccgtttttca tgtggtcttc
      721 tttcattcgc ataaaggtgg catcagtatc tgtcttggag tagctattac gttcagctag
      781 cagttcttct tgtttttcat acttctgcat acggggaagg taatctttct cgatcaattt
      841 tttggccttt ttcagtgttt tgtcggcagg atttgctgcc agtctttcat caattcgttg
      901 taccgcttct tctatggctt ttgcatctat gggtttttct cctagctctt caaggttacg
      961 atccccatat tccatatttt cttcatcatt tattagttct gccatggcaa aaagttcctt
     1021 gcatttttcc cttagttttt ctttatagcg tttggttgat ttaccccaaa cccagctata
     1081 tttattagcg ttggcttcga tttttgttcc atccaggaag tagttttcta atttgatgta
     1141 accttgctta atcaacaact ccacgacttc actaaatatg tccaaaataa catctttcat
     1201 tcgttcccca cgaaagctat taatggtttt aaagtctgga gcattaccac cgcatagcca
     1261 catatacatg atgttttctc tagctgcttt ttctatccgt cggcttgaat atattttatc
     1321 tgcataggca tatacgatga gttttgtcat cattacggga tggaagctgg ccctcccgcc
     1381 tccagggtat ttagcaagca gcgggtcaag attcatctga tctatggccg aatttatgac
     1441 ccttaccata tgattagtcg gaatcatgct ttccagatcc attggcaaac ttaattgctg
     1501 ctgattatat tctttgaaca cgaccttgtt atgtactttc tgccctggct ttgacatgtt
     1561 ttttacacct gcttttttat caattctact tcttatattt taccagtttt cttccctcct
     1621 gaaattacaa aaccgcctaa aaccttaagt tttaagcggt cttgtgaact gtgttttcac
     1681 acagcctctt gctgtttctt atagtggttc gacatattaa tgattatttc gcaggtgaaa
     1741 aatatgaagg ataagctttt atgcctggtg gaggaagaag gcatttatct attacattgg
     1801 gattt
//
LOCUS       DAORPD010000371         1804 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_12292_length_1804_cov_2.408805, whole genome shotgun sequence.
ACCESSION   DAORPD010000371 DAORPD010000000
VERSION     DAORPD010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12292_length_1804_cov_2.408805"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1330
                     /locus_tag="PK976_05025"
     CDS             <1..1330
                     /locus_tag="PK976_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67283.1"
                     /translation="SGLSFGKSGITGGMPHTARGLTPSTSSNEIYYLRGGGITLGLGN
                     FEISTLASRIALDASTSVLDTLEPSEIIEGLRTTGLHRTISEVDLSKNIHLTTLGGNI
                     SYTGNIFKAGFTIASENFDKAFNYQTGLYNLFQQPRKSQFTIGTDVCIYLRDFVLQGE
                     IARDVEGHLAGIFNANFQPDPRLTLAFIYRNYDKAFQNYHSNAFSENSGVYNEQGVYI
                     GFRASVAKQLELTGYADYFLFPWFKYRVDGPSKGREYFIRMDLLMDEKTRAYLRYRFE
                     QKEINTKEENRKLNTLSPRKYHNVRMHFDYQPIEEWKFASRAEAIFNYTLEGNSRMGF
                     LIYQDVTWQPFRLPISISGRYALFDTETYDERLYAYEHDMLYSFSIPAYYYKGQRIYL
                     ILKYSLYKNLNFWLRFASSIYWNKDEIGTGLEIIHGNTRSEIKAQLIWKF"
     gene            1457..>1804
                     /locus_tag="PK976_05030"
     CDS             1457..>1804
                     /locus_tag="PK976_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005925906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRC67284.1"
                     /translation="MDKVKILWVDDEIDLLKAHVIFLEQKGYEVHTSNNGNEALDMMR
                     NVPYDIVFLDENMPGLTGLETLEIIKQINPNLAVVMITKSEEESIMEEAIGSNISDYL
                     IKPVKPNQILLSIK"
BASE COUNT          565 a          346 c          366 g          527 t
ORIGIN      
        1 gagcggtttg tcattcggga agtcaggaat tacaggtggt atgcctcata ctgcccgggg
       61 tcttactcca agcacctcat ccaatgaaat ttattatttg agaggtggag gaattacgtt
      121 aggactgggc aatttcgaga taagtactct ggcttcacgt attgccctcg atgcgagtac
      181 atcggtactg gatacgttag agccatctga aatcatcgag ggacttcgaa ctactggcct
      241 gcaccgcaca atatctgaag tggatcttag taaaaacata catcttacta ctcttggagg
      301 aaatatcagt tatactggaa atattttcaa ggccggtttt acaattgcat cggaaaattt
      361 cgataaggca ttcaattatc aaacaggcct ttacaattta tttcagcaac ccagaaaatc
      421 acaattcacc atagggactg atgtttgcat atatttacgc gattttgttc tacaaggcga
      481 aattgccaga gacgtggaag ggcacttagc gggaattttc aacgctaatt ttcagcctga
      541 tccacgactc acattggcgt tcatataccg aaattatgac aaggctttcc aaaactatca
      601 cagcaatgct tttagcgaaa attcgggtgt atataatgaa cagggagtat acataggatt
      661 tagggcatct gtagccaaac aacttgaatt gacaggctat gccgactatt tccttttccc
      721 atggttcaag tatcgggtcg atggcccttc gaagggaagg gaatatttca ttagaatgga
      781 tttattaatg gacgaaaaaa ccagagctta tcttcgctac agattcgagc aaaaagaaat
      841 taatacgaaa gaggaaaacc gaaaacttaa tacactttcc cctcgtaaat atcacaacgt
      901 aagaatgcat ttcgattatc aacccattga agaatggaag tttgcctccc gagcagaagc
      961 cattttcaat tacactttgg aaggaaattc aagaatggga tttcttattt atcaggatgt
     1021 tacctggcaa cctttccgac ttcccatttc catctcgggt agatatgctc tcttcgacac
     1081 ggaaacttac gacgagcgtt tgtatgctta tgaacatgac atgctataca gcttctccat
     1141 tccggcttat tattataaag gacagagaat ctatttgatt ttgaaatatt ccctctataa
     1201 gaaccttaac ttttggttgc gttttgcttc atccatttat tggaataagg atgaaattgg
     1261 cacgggtctt gaaattattc atggaaacac tcgaagcgaa atcaaagccc agcttatctg
     1321 gaaattttag tcatttatca gagcgtgaaa taaacttttt tgaaagccgt tgatatattt
     1381 atcgggctaa tgatttgtga aggctttgtg ctacaataat aaaatatttt cccttcatta
     1441 taaatggaga tagtttatgg ataaagtcaa gatactatgg gtggatgatg aaattgatct
     1501 gttgaaggct catgtcatat ttttagaaca gaagggctat gaggtgcata cttccaacaa
     1561 tggcaacgaa gctttggata tgatgcgcaa tgtaccttac gacattgttt ttcttgatga
     1621 gaatatgccg ggactcacgg gtctggaaac gctcgaaatc atcaagcaaa tcaatcccaa
     1681 ccttgccgtg gtgatgatta ccaaaagcga agaagaaagc ataatggaag aagccattgg
     1741 aagtaacatc tctgattacc tcattaagcc cgtaaaaccc aatcagattc tattatccat
     1801 caaa
//
LOCUS       DAORPD010000372         1803 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_12307_length_1803_cov_2.533753, whole genome shotgun sequence.
ACCESSION   DAORPD010000372 DAORPD010000000
VERSION     DAORPD010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12307_length_1803_cov_2.533753"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="PK976_05035"
     CDS             complement(<1..290)
                     /locus_tag="PK976_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67285.1"
                     /translation="MKKNSKSKNALLWIISFIIMTAASVYQRLTGPTHPVQAKIEIEG
                     TSYLFKLIRSHGSKADARIVLSIPDTSIHGIIAYKKINATETWENDSLVRLG"
     gene            complement(298..1224)
                     /locus_tag="PK976_05040"
     CDS             complement(298..1224)
                     /locus_tag="PK976_05040"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HRC67286.1"
                     /translation="MNHRPHTHNAHTQNTSQQRLGLTIGLNLMIAMVEVVGGIISGSL
                     ALISDSVHNLADSVSLIISYFALKLSKKKVSLRNTFGYHRAEILAAFINATILAGITL
                     FLIVQAFIRFFRPVQIKVDTMGIVALIGLGANLAGMILLKKPSSQSLNIRSSYLHLVG
                     DTLSSVAVIIAALLIELTKLTFIDPLITIIISLYIIKETWSVLQRSTDILMMASPTDI
                     NLLKIQREIEKMEGIDNIHHVHIWQLDDKTIHFEAHLRLAKNPSIHEADQIRQKIERW
                     LIDNNIQHVTLQIEPSETANACPSNSLLGSTH"
     gene            complement(1296..>1803)
                     /locus_tag="PK976_05045"
     CDS             complement(1296..>1803)
                     /locus_tag="PK976_05045"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRC67287.1"
                     /translation="HERIAPTLVDTEFRKQLLHGYVHDPAAAMLGGVSGHAGLFAHAF
                     DVARIMQMLLWKGEYNHIRFFQKETVNYFTSNVEDGNNNRRGLGFDKPPKNFKSSSSS
                     VCSLASPKSFGHSGFTGTYVWADPDHDLIYVFLSNRIHPSDENEMINKLNIRTRIHQA
                     AYEAIIDY"
BASE COUNT          543 a          333 c          384 g          543 t
ORIGIN      
        1 tttcccaacc tcactaaact atcattttcc catgtttctg tggcattgat cttcttatat
       61 gctattatgc catgaatgga tgtatcggga attgaaagaa ctatcctagc atcagcctta
      121 cttccatgac tacggattaa tttgaagaga taagaagttc cttctatttc tattttggcc
      181 tgtactggat gagttggccc tgtaagacgt tgatatacag aggcagccgt cattataatg
      241 aaagagataa tccagagaag ggcatttttc gacttcgagt tttttttcat aatgtattca
      301 atgcgttgaa cctaataatg aattcgaggg gcaagcattc gcagtttcgg atggttcgat
      361 ctgaagagta acatgctgga tattattatc gatcaaccat ctctcgattt tttgtcttat
      421 ttgatcggct tcatgtatag aaggattttt cgcaagtctt aaatgagctt cgaaatggat
      481 ggttttatcg tcaagctgcc agatatgcac atgatggata ttgtctatgc cttccatttt
      541 ttcgatctcg cgttgtattt tcagaagatt gatatccgta ggagaagcca tcatcagaat
      601 atcagtggaa cgttgcagaa ccgaccatgt ttctttgatg atatataagc ttatgatgat
      661 ggtaatcagg ggatcgataa aagtgagttt cgtcagctct ataagcagag ctgcaatgat
      721 aactgctacc gacgaaagag tatctccaac gagatgaagg taagatgatc gaatgttcag
      781 gctttgagaa gaaggtttct ttagtaatat catgcctgct aaatttgctc ccaatcctat
      841 aagggcaact atccccatgg tatcgacctt aatttgtaca gggcggaaga atcgaataaa
      901 agcctgaacg attaaaaata gtgtgatgcc agctaaaatg gtagcattaa tgaaagcggc
      961 caaaatttca gcacgatgat aaccaaaagt attacgaagg ctaactttct tcttagataa
     1021 tttaagggcg aaataactga taattaacga tacactatcg gccaagttat gaacggagtc
     1081 ggagatcaag gccaatgaac ccgatataat tcctcccacc acttccacca ttgcaatcat
     1141 caaatttaaa ccaatcgtca atcccagacg ttgctgagag gtattctgag tatgggcgtt
     1201 atgcgtgtgg ggtcgatggt tcatgagagg agaactattt ttattatagt aagtttttcg
     1261 agatgacagt taatttaaga atatttcttt gatggtcaat aatcgatgat agcctcgtag
     1321 gctgcttgat gaattcgggt tcggatgttg agtttattga tcatttcatt ttcatcggag
     1381 ggatggatac gattgcttaa aaaaacataa atcaggtcgt gatcgggatc agcccataca
     1441 taagtacctg taaacccaga atggccaaaa ctttttggag aggctaagga acaaactgag
     1501 ctgctgctgg atttgaaatt ttttggaggt ttatcgaaac ccagtccacg acgattatta
     1561 tttccatctt cgacattaga agtaaaataa ttgacggttt ctttttgaaa aaatcgaata
     1621 tgattatact cacctttcca gaggagcatt tgcataatac gcgccacatc gaaagcatga
     1681 gcaaataggc cagcatgtcc cgaaacacct cctaacatgg cggcagccgg atcatgcaca
     1741 tacccatgaa gaagttgttt gcgaaattct gtatcgacta acgtaggggc aatccgctcg
     1801 tgc
//
LOCUS       DAORPD010000373         1797 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_12367_length_1797_cov_2.284156, whole genome shotgun sequence.
ACCESSION   DAORPD010000373 DAORPD010000000
VERSION     DAORPD010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12367_length_1797_cov_2.284156"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..1549
                     /locus_tag="PK976_05050"
     CDS             158..1549
                     /locus_tag="PK976_05050"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRC67288.1"
                     /translation="MNDKDRPTLLLVYPANPLRKGFLSNRDTKYQPLSLGIIAALTPP
                     HWKVKLIDENFRSFKYYPADLVGITAFSSTVTRAYEIARIYREKGIHTVIGGIHASMM
                     PDEALQCVDTVVIGEAESVWPKVISDFEAGCIQRIYKGELLEMINSPKPRRDLFHPGY
                     VFASIQTTRGCPFDCSFCSVSAFNGRKYRMRPIEEVLDELEEIPQNNVFFLDDNIVGI
                     TKEHQERAIELFRRIIERRIKKNWISQASINLADNPEVLKWAARSGCRMIFIGVESEA
                     EDGLRESNKRINLKYGVSSYEKVFQKVHQHGIAVIAGFMFGWDSDTAEKMQQRVEYIR
                     HSQADSIQTSIVTPLPGTRLYHKLMAENRIILKNYPDDWQRYDYWEIVHSLPMMAKQE
                     FETEMQRVYRRIYEPTFLRQKFFRSIWRLKSIITAYWSYMSNWNYRNMYIHDGKKARN
                     FLLDPFPFFRKKL"
BASE COUNT          540 a          389 c          370 g          498 t
ORIGIN      
        1 aatttctgcg tacactctgg aatactaaaa acttaagaac ttcagtttgg gcatggaaca
       61 gcaacctcaa ctacagggca gtttccatgg aaaagcctcc agccgttggc catgatatcc
      121 aaaacacttc ttcagaagct gattatattc attgaaaatg aacgataaag atcgccccac
      181 cctattgctg gtttatccag ccaatccgct caggaaagga tttttatcga atcgcgatac
      241 aaaatatcaa ccgctgagtt taggaattat tgccgcctta actccccctc actggaaagt
      301 gaagctgata gatgaaaatt ttcgttcttt caagtattac cctgccgatc tggtcgggat
      361 taccgcattt tcgagcacag ttacacgggc ttacgaaatt gctcgaattt acagggagaa
      421 gggtatacac acggttattg gcggaataca tgccagcatg atgcctgatg aagctctgca
      481 atgtgtggat acagtggtaa ttggagaggc tgagagtgta tggccaaaag taatctccga
      541 ctttgaagcc ggatgtattc aacgcatata taagggagaa ctactcgaaa tgatcaatag
      601 cccgaagcca cggcgcgatt tgttccaccc gggttatgta tttgcttcca ttcaaaccac
      661 tcgtggctgc cctttcgatt gtagtttttg ttccgtatcg gctttcaatg gacgcaagta
      721 tagaatgcgc cctatcgaag aagtattgga cgaattagaa gaaattcctc aaaataatgt
      781 cttctttctc gacgataata ttgttggcat caccaaagaa catcaggaaa gagccataga
      841 gctattcaga agaatcatcg aacgcagaat taaaaaaaac tggattagtc aggcatccat
      901 taatcttgcc gataatcccg aagttctcaa atgggcagcc cgaagtggct gtcgaatgat
      961 cttcattgga gtagaaagtg aagcagaaga cggactccgg gaatcgaata aacgcatcaa
     1021 cctcaaatat ggggtttcat cctatgaaaa agtttttcaa aaagttcatc aacacggtat
     1081 agcagtgatt gcaggcttta tgttcggttg ggactcggat acagccgaaa aaatgcaaca
     1141 gagagtcgag tatattcgtc attctcaagc cgactccatt caaaccagca tcgtaacccc
     1201 attaccagga acacggcttt atcataaatt aatggctgaa aaccgtatta tcttgaaaaa
     1261 ttatcctgat gactggcaac gttatgatta ctgggaaatt gttcacagtc taccaatgat
     1321 ggccaagcaa gagtttgaaa cagaaatgca aagggtatat cgacggattt atgaacctac
     1381 gttcctccga caaaaatttt tcaggtcgat ttggaggttg aaaagtatta ttaccgccta
     1441 ttggagttac atgagcaatt ggaactatcg taacatgtat attcacgatg ggaaaaaggc
     1501 aaggaacttt cttctcgatc catttccatt ttttcgtaaa aagttataga aaagaccaag
     1561 tcgcctcgtt ccccactctc ttggtttact catctctttg gccaactggg ctttactaaa
     1621 cttccactcc aacttgattc tttccttcct tattcctcac atttcacgca atgctcagct
     1681 aaactcagta aacgagggaa atggcgatag agcatttaca tcatttcttt cacggctttg
     1741 atgatgcctt ccttgtcgta gccacactct ttctgcaatt gttcgatggt gccatgg
//
LOCUS       DAORPD010000374         1795 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_12385_length_1795_cov_2.760345, whole genome shotgun sequence.
ACCESSION   DAORPD010000374 DAORPD010000000
VERSION     DAORPD010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12385_length_1795_cov_2.760345"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            183..1091
                     /locus_tag="PK976_05055"
     CDS             183..1091
                     /locus_tag="PK976_05055"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRC67289.1"
                     /translation="MRQDGKFSAFVYLLVILAMVFWGMSYVWTTMVFRYLNPITTIFL
                     RLVISSALLFLIFGLTHQLQPVRKKDYKLFLFAALFEPFLYFLGEGYGVKYSTPTISA
                     IIISTIPIFSAIAASIFLKEVLQPLQIFGFFLSLGGIIFMLLGPNFSFQFNPIGIVSL
                     FFAVATAVIYAVLLKRLTMLYSSFTIVAWLNLIGAFYFLPLVLIVEPSQLTLLHFEWG
                     LWYPLIMLAVFASSLAFIFFTFGMHHLGVSRTSIFTNFIPVFTAIFSYFKIHEKLTVN
                     TLVGMLMVIVGVIITQKNVRIKTLRA"
     gene            1164..1237
                     /locus_tag="PK976_05060"
     tRNA            1164..1237
                     /locus_tag="PK976_05060"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1198..1200,aa:Met,seq:cat)
     gene            complement(1492..>1795)
                     /gene="rfbC"
                     /locus_tag="PK976_05065"
     CDS             complement(1492..>1795)
                     /gene="rfbC"
                     /locus_tag="PK976_05065"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF013103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HRC67290.1"
                     /translation="VDLRKGSPTYGQWDSIVLSESNKFMYWIPEGMAHGFRTLQDDTL
                     FFYKCTHFYNKGSEQALRWNDPALNIDWQLTEPPILSEKDKLSPFFKDFVSPFEYE"
BASE COUNT          484 a          328 c          329 g          654 t
ORIGIN      
        1 ccaatctcca atccccaatt tccaatctcc aatccccaac ctctattctt acttttttcc
       61 ttcctctgtt ttcccttcct tttgtcaaaa tcaggaaatt ttttcccctt gattttaaat
      121 tttttcaaga tgcagctgga aggtgtttgt acttttgcaa taccaactca agatgcttag
      181 ggatgagaca agatggtaaa ttttcggctt ttgtctattt attggtgata ttagctatgg
      241 ttttttgggg tatgtcgtat gtatggacaa ccatggtgtt tcgatattta aatcctatta
      301 cgacaatttt tcttcgattg gtcatcagta gtgctctact ttttcttatt ttcggactta
      361 ctcatcaatt gcaacctgtt cgaaagaaag attacaagtt attcctattt gctgcattat
      421 ttgaaccatt cttatatttt ttgggtgaag gatatggagt gaagtattcc actccaacca
      481 taagtgctat tattatttct accattccta ttttttcggc tattgcggct tctatttttc
      541 tgaaggaagt tttacaacca ctgcaaattt ttggattctt cctttcgtta ggaggaatta
      601 tttttatgtt gttaggcccc aatttttctt tccaattcaa tccaattgga attgttagtt
      661 tgttttttgc tgttgctact gcggtaattt atgcagtttt gctgaaaaga ttgactatgc
      721 tttattcttc tttcaccatc gtagcatggt taaacctcat tggcgcattt tattttctcc
      781 cattggtatt gatagtcgaa cccagccagc ttactttgct tcatttcgag tggggattat
      841 ggtacccact gatcatgctt gctgtttttg catcttcact tgcattcatc ttttttactt
      901 ttggcatgca ccatcttggt gtgagccgca cgagtatctt tactaatttt atcccagtat
      961 ttacagcgat attttcctat ttcaagattc acgaaaaatt gacggttaat actttagtag
     1021 gaatgttaat ggtaattgtc ggtgtgatta tcactcaaaa aaatgtcaga ataaaaacac
     1081 tgagagcata gcaaaagatt agaagtgagc ggaggattgt ttgcaagctg gattatttta
     1141 tctaattttg ccgaactttt taaggcgggg tagctcaggt ggttagagcg tcggattcat
     1201 aacccggagg tcacgagttc aaatctcgtc cccgctacta aaggctttgc ctacaatgta
     1261 aggctttgtt ttatttcctt cttataagtt tttaaaaatt tccttcctat cctcaacctg
     1321 tgctcatctg agattctatt gattgttgaa agaattgtgg taaacataga attagttgaa
     1381 aaattaaccc ccctctggaa taagagctaa attttaaaag agaaaaaaat acaggatatt
     1441 gttaaaaaga agattgatga gtattaacta atataaaatg aagatatggt cctattcata
     1501 ttcgaaaggg ctgacaaaat ccttgaagaa tggtgaaagc ttgtcttttt cggaaagtat
     1561 gggtggttct gttaactgcc aatcgatgtt caaagcggga tcattccagc gaagagcctg
     1621 ttccgatccc ttattataga aatgcgtgca tttgtagaaa aacaaggtat cgtcttgcag
     1681 ggttcgaaag ccatgagcca ttccttcggg aatccaatac atgaatttat tgctttcgct
     1741 aagtacgatt gaatcccatt gaccataagt aggagaacct ttacgtaaat cgacc
//
LOCUS       DAORPD010000375         1795 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_12388_length_1795_cov_2.222989, whole genome shotgun sequence.
ACCESSION   DAORPD010000375 DAORPD010000000
VERSION     DAORPD010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12388_length_1795_cov_2.222989"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            87..692
                     /gene="amrA"
                     /locus_tag="PK976_05070"
     CDS             87..692
                     /gene="amrA"
                     /locus_tag="PK976_05070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HRC67291.1"
                     /translation="MVGYHAMTITQKLSSNDFSLTDTDKKNLLKIARQSIEQYFKTKT
                     RLPKPDALLTPNLLKPCGAFVTLHKHGELRGCIGHFGEDKPLWETVAEMALASAFNDT
                     RFYPLSEEELREIEIEISVLTPMRKISSINEIQLGKHGIYIKKGWRSGTFLPQVATET
                     GWTLEEFLGHCSRDKAGLGWDGWKDAEIYVYEAIVFSEKEF"
     gene            complement(696..>1795)
                     /locus_tag="PK976_05075"
     CDS             complement(696..>1795)
                     /locus_tag="PK976_05075"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HRC67292.1"
                     /translation="GGLGGGEKSRFILQIENEFPLNVKNTKISTTTVSFRYPSIPHEE
                     IVFTKTPEVMKKLTYKAQDGLSPSALWTFNVCGVKFYFDYMVGLIDYQITEGEIDEAV
                     FGTVVHKVLQEFYPHEYPFRVTEEFINNKLTQLDDLIRKSFQSELEGIDIDYGRNKLL
                     FGVAQQILTRFFQNEIEFLKDYPDLQILNLEKVITYTMPFPLSVDMDGSDISLDSVVI
                     HGRIDRIDQVGEQIRIIDYKTGKGLDKKLSLDISKNLSNKLDTDKAFQLLTYLWMYKM
                     NNPKLNDVKAGIMFLRRSDIGFTEAIIKNPSHSNDNPVQFNSSIQSIQYIEQQIKMLL
                     RNLFDLNQSFVQTSNKNACRYCKYKFICARG"
BASE COUNT          585 a          362 c          328 g          520 t
ORIGIN      
        1 acattacttt cgaattaccc ggaatcgaaa tcaaagacat cgactatcaa aattaaggtc
       61 acagtcccta tggcgaccaa gaccacgtgg taggctatca cgccatgacc ataacccaaa
      121 aacttagttc gaacgatttt tcgcttaccg ataccgataa aaagaatcta ttgaagattg
      181 cacgtcaaag catagaacaa tatttcaaga cgaaaacgcg actgccaaaa cccgatgcac
      241 ttcttacccc gaatctgctg aaaccctgcg gagcttttgt tacacttcat aaacacggag
      301 aactaagagg ctgtataggc cattttggag aagacaaacc actgtgggaa actgtagccg
      361 aaatggctct tgcatcggct ttcaacgata cgcgatttta tcccttaagc gaagaggaac
      421 tcagagaaat tgaaatagaa atttcggtgc ttacacccat gcgcaaaatc tcatccatca
      481 acgaaattca attaggaaaa catggcatct acatcaaaaa aggctggaga tcgggtacat
      541 tcttgcctca agtagcaacc gaaacaggct ggacactcga agaatttctt ggccattgta
      601 gccgcgataa agctggcttg ggttgggacg gttggaaaga cgccgaaatc tatgtttatg
      661 aagctatcgt tttcagcgaa aaagagtttt aatcttcacc cgcgggcaca aataaacttg
      721 tatttacaat aacgacaggc attcttgttt gaagtctgga caaaggattg attaaggtcg
      781 aaaagatttc taagcaacat ttttatttgt tgctcaatgt attgtataga ttgtatagag
      841 gaattaaatt gaacaggatt atcatttgaa tgggaaggat tttttattat ggcctcagta
      901 aaaccaatat cagatcttct aagaaacatt atgccagcct tcacatcatt caatttcgga
      961 ttattcatct tatacatcca taaataagta agcaattgaa aggctttgtc tgtatctaat
     1021 ttattggaaa gattttttga aatatctaaa gacaatttct tatctaagcc tttgcctgtt
     1081 ttgtagtcta tgatgcgaat ttgctcgcct acttgatcga tgcgatcgat tcggccatga
     1141 atgactaccg aatctaaaga gatgtcacta ccatccatat caactgataa tggaaaaggc
     1201 atggtataag tgattacctt ttcaagattc aaaatttgca aatcgggata atcctttaaa
     1261 aactcgattt cattctgaaa aaatcttgta agaatttgtt gagccacgcc aaaaagcaac
     1321 ttattacgtc catagtcgat atctatgcct tcgagctcac tctggaaact cttcctgatt
     1381 aaatcatcta attgagtaag tttattattt ataaattctt cagttacacg aaatggatac
     1441 tcatgaggat aaaattcctg taaaacttta tgaaccaccg tcccaaaaac agcttcatca
     1501 atttctcctt cggtaatctg ataatcgatc aaacccacca tatagtcgaa ataaaatttt
     1561 acaccacaaa cgttaaaagt ccacaaagcc gaaggcgaaa gcccatcttg agctttatac
     1621 gtcaattttt tcatgacttc gggagtttta gtaaaaacta tttcctcgtg aggaatgctg
     1681 gggtagcgaa agctcaccgt ggtggttgag attttcgtat ttttcacatt taaagggaat
     1741 tcgttttcga tttgaaggat aaagcgactt ttttcacctc cgcccaaacc cccgg
//
LOCUS       DAORPD010000376         1794 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_12394_length_1794_cov_2.175963, whole genome shotgun sequence.
ACCESSION   DAORPD010000376 DAORPD010000000
VERSION     DAORPD010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12394_length_1794_cov_2.175963"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1794)
                     /gene="mutS"
                     /locus_tag="PK976_05080"
     CDS             complement(<1..>1794)
                     /gene="mutS"
                     /locus_tag="PK976_05080"
                     /inference="COORDINATES: protein motif:HMM:NF003810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HRC67293.1"
                     /translation="GDFYETFGEDAIKASQILGIVLTKRSNGAAAMVDLAGFPYHALD
                     NYLPKLVKAGYRVAICDQLEDPKLAKTIVKRGITELVTPGVSTDDRILDQKENNFLAS
                     IQLGENLSGISFLDISTGEFLVAQGNNEYIDKLLQNFKPSEIILQKNKLHRFIEIFGD
                     KYYINTFDDWVFTEDFARETLLRHFQVVSLKGFGIEIFPLAIIAAGAAMHYLAETQHD
                     RLSHITRIARIEEEKYVWLDRFTIRNLEIVQTFNENARSLLQILDQTISPMGARLLRR
                     WITMPIKERKVLEERLDLVELLISDHELAEFLATHLKTVGDLERLISKVSIGKANPRE
                     VLYIARGLEAIERIASRCLLTHHPVLMQLCEQLNPCKLIRDRIFKTLKEDCPPVLIKG
                     NIIAERVDEELDHLREILYQGKDVLAKIQQREIQTTGISSLKIGFNNIFGYYIEVTNA
                     HKNKVPSTWHRKQTLVNAERYITEELKEYEQKILNAEEKIVELENRIFNELLSSVAEY
                     VEPLQLNAGLLARLDVFLSFAQLSASSNYTRPVLNDGLNIDIRAGRHPVIEKQLPAGE
                     QYIPNDVVLNPNNQQIIILTGPNMAGKSAFLR"
BASE COUNT          487 a          361 c          386 g          560 t
ORIGIN      
        1 cgaagaaagg ccgattttcc tgccatgtta ggaccggtaa gaataataat ctgctgatta
       61 tttggattca gtactacatc attagggata tactgttctc ctgcaggaag ttgcttttcg
      121 atcactggat ggcggcctgc gcggatatcg atgttcaatc catcatttag cacaggtcgg
      181 gtataatttg aacttgccga aagttgtgca aacgacagga aaacatctaa acgcgctagt
      241 aatccagcat tcaattgcaa aggttccaca tattcggcta ccgagctaag taactcatta
      301 aaaattctgt tttcgagttc tacaattttt tcttcggcgt tgagaatctt ttgttcgtat
      361 tcctttagtt cttcggtaat atatctttcg gcgttcacta atgtttgctt gcgatgccaa
      421 gtggatggta ctttattttt atgtgcattg gttacttcta tataatagcc aaagatatta
      481 ttgaatccaa ttttgaggga tgaaattccg gtagtttgga tctctctttg ctggatttta
      541 gcaagcacat ctttgccctg atagagaatt tcacgtaaat ggtccaattc ttcatcgact
      601 ctttcggcaa tgatatttcc cttgatcaat actggtgggc aatcttcctt gagtgtttta
      661 aatatgcgat ctctgatgag tttgcagggg ttcagttgtt cgcataattg catcaataca
      721 ggatgatgtg taagaagaca gcgcgaagca attcgttcga tggcttcgag cccccgggca
      781 atataaagaa cttcacgtgg attggctttt cctatgctta cttttgaaat caggcgttcc
      841 aaatcgccaa cagttttgag atgggtagcc agaaattcgg ccagttcatg gtcggaaata
      901 agaagttcta ccaggtcgag acgttcttca agtactttac gttcctttat aggcatggtg
      961 atccaccgcc ggagcaggcg ggctcccatc ggactgatgg tttgatcgag aatttgaaga
     1021 agtgagcggg cattttcgtt gaaggtctga acgatttcga gatttcgaat cgtaaatctg
     1081 tccagccaaa cgtatttttc ttcttcgata cgggcaatac gagtgatgtg gctcaaacga
     1141 tcatgctgag tttcggcaag ataatgcata gcagctccag cggctatgat agctaacgga
     1201 aatatttcta tcccgaatcc tttgagggaa acaacctgaa aatgcctcag caaagtttca
     1261 cgcgcaaaat cttcggtaaa tacccaatca tcaaaggtat ttatgtaata tttatctccg
     1321 aaaatctcga taaaccgatg caatttattc ttttgaagaa tgatttcgct gggtttgaaa
     1381 ttttgcagaa gcttgtcgat atattcatta ttaccctgag ctacgaggaa ttcacctgtg
     1441 gagatatcca aaaaacttat gcccgaaagg ttttcgccca gctgaatgct ggcaagaaaa
     1501 ttattttcct tctgatcgag aattctatca tcggtgctca caccaggagt aaccaattca
     1561 gtaattcctc ttttgactat ggtttttgcc agtttggggt cttccagttg atcgcaaata
     1621 gccactctat agccagcctt gaccagtttt ggtaagtaat tatcgagagc atgataggga
     1681 aatcctgcta aatcaaccat ggcagcggca ccattcgatc ttttagtaag gacaatgcca
     1741 aggatttgag aggcttttat ggcatcttcg ccaaaggttt cataaaaatc accc
//
LOCUS       DAORPD010000377         1790 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_12435_length_1790_cov_2.396542, whole genome shotgun sequence.
ACCESSION   DAORPD010000377 DAORPD010000000
VERSION     DAORPD010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12435_length_1790_cov_2.396542"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /gene="yihA"
                     /locus_tag="PK976_05085"
     CDS             <1..502
                     /gene="yihA"
                     /locus_tag="PK976_05085"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HRC67294.1"
                     /translation="NVGKSTLINMLLQCRGLAKTSSTPGKTRTINHYLVNRSWYLADL
                     PGYGYARLSKIERKALSNMISSYLQSRKNLLNTFILIDARISPQPIDIDFINIFGEYQ
                     LPFCLVFTKTEKLGQMVLQQQIDAFLETLSPFWDEFPKYFITSGKTKVGRDEILDFIN
                     DTNLIM"
     gene            complement(592..969)
                     /locus_tag="PK976_05090"
     CDS             complement(592..969)
                     /locus_tag="PK976_05090"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HRC67295.1"
                     /translation="MNEENLPIPSEELPPIEMVKVNVDGSTFLTTLSPKFKLSKPYKI
                     NEPDKITAFIPGIIINVLVKKNQVVRKGDKLLILMAMKMNNHIRAPFDAIVKKIYVKK
                     GDQVKKDQLLVELQPILELNSIN"
     gene            complement(1001..>1790)
                     /locus_tag="PK976_05095"
     CDS             complement(1001..>1790)
                     /locus_tag="PK976_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRC67296.1"
                     /translation="SKAKPFARKPPKRTYKIDDIIPADTTEPYDVKDVIRSIVDDSEF
                     LEVHELFAPNIVVCFARIAGQSVGIVANQPMVLAGVLDVDSSDKAARFIRFCDAFNIP
                     LVSLVDLPGYLPGIDQEHAGVIRHGAKVLYAYSEATVPKITIILRKAYGGGYIAMCSH
                     HLKADFVLAWPTAEIAVMGPEGAANIIFRKEIMEAENPEEMRKQKIEEYKAKFANPYV
                     AAAYGYVDAVIEPSETRHFLTHALEISVSKSETLPRKKHGIPPF"
BASE COUNT          496 a          366 c          373 g          555 t
ORIGIN      
        1 gaatgtaggc aaatctaccc ttatcaatat gttgctccag tgcagaggtt tggccaaaac
       61 ttcttcaacc ccgggcaaaa ctcgcactat caatcattat ctggtgaatc gttcatggta
      121 tctcgccgat ttacctggct acggttatgc tcgattatcg aaaatagaaa ggaaagcact
      181 cagcaacatg atatcatcct atctccaatc tcgaaaaaat ctgctcaata cattcattct
      241 tatcgatgct cgaatttcac cccagcctat agatattgat tttatcaata ttttcggtga
      301 atatcaattg cctttttgcc ttgtatttac aaaaaccgaa aaattgggac aaatggtttt
      361 acagcagcaa atcgatgcat ttttagaaac tttgtcacct ttctgggatg aatttcccaa
      421 gtatttcatt acctcgggga aaactaaggt tggtcgcgac gaaatccttg attttatcaa
      481 cgacacaaac ctcattatgt agcctgcaaa aaaaaaatcc tcgaaaaaac taaatttccg
      541 ggggttcgca tgaagttcat ttggaaaatg ggcgatgggg aggttaagca gttaattaat
      601 agaattcaat tcaaggatgg gctgaagttc aacgagaagt tggtcttttt ttacctgatc
      661 gccttttttg acgtaaattt ttttcactat ggcatcgaag ggggcacgga tatgattatt
      721 cattttcatg gccataagaa tcaataattt atctcctttc cgtacgactt gattcttttt
      781 tactagcaca ttgatgatta taccgggaat gaaggccgta atcttatccg gctcatttat
      841 cttgtaaggt ttactaagtt taaacttagg acttaaggtg gtaagaaagg tcgatccgtc
      901 aacattgact tttaccattt ctatgggagg taattcttct gatggaatgg gtaaattttc
      961 ttcgttcatg ggctattgtt ttaattcaac caataattaa ttaaaaggga gggattccgt
     1021 gctttttgcg tggtagggtt tctgattttg agacagatat ttcgagtgca tgagttagga
     1081 aatggcgagt ttcgctaggt tcgattacgg catccacata gccataagct gcggctacgt
     1141 atggattggc aaattttgcc ttgtattctt cgattttttg tttgcgcatt tcctcgggat
     1201 tttcagcttc catgatttct ttacggaaga tgatgttagc cgctccttca ggacccataa
     1261 cggcaatttc tgcggtaggc catgctaaaa caaagtctgc cttcaaatgg tgagaacaca
     1321 tggcaatata tcctccgcca taagccttgc ggaggattat ggtaatcttg ggaaccgtgg
     1381 cttcagaata agcatacagc actttggctc catgcctgat cacaccggca tgttcctgat
     1441 ctattccagg cagataaccc ggaagatcga ccagtgaaac caaaggaata ttgaatgcat
     1501 cgcaaaaacg gataaatcgt gcggctttgt cgctactatc tacatcgagt acacccgcca
     1561 gaaccattgg ctggttcgct acaatgccca cgctttggcc cgctattctg gcaaagcaga
     1621 ctacaatatt gggggcaaac aactcgtgca cctcgagaaa ttccgaatca tccacaatgg
     1681 atctgataac gtcttttaca tcatatggtt cggttgtatc ggcaggaata atgtcatcga
     1741 ttttataagt tcgcttgggt ggtttcctgg cgaaaggctt agccttgctt
//
LOCUS       DAORPD010000378         1781 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_12525_length_1781_cov_2.335458, whole genome shotgun sequence.
ACCESSION   DAORPD010000378 DAORPD010000000
VERSION     DAORPD010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12525_length_1781_cov_2.335458"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1287)
                     /locus_tag="PK976_05100"
     CDS             complement(<1..1287)
                     /locus_tag="PK976_05100"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HRC67297.1"
                     /translation="MAIEDKLLELLEKRQKASLGGGQKRIDAQHAKGKLTARERIDLL
                     LDDGSFEEFDMFVTHRCTDFGMQDQLYLSDGVVTGHGTIDGRVVYVFSQDFTVFGGSL
                     SESYAKKICKIMDQAMKVGAPIIGLNDSGGARIQEGVLSLAGYAEIFERNILASGLIP
                     QISAIFGPCAGGAVYSPALTDFIIMSQKTSYMFVTGPKVVKTVTGEVVTEEELGGAMI
                     HGAKSGVAHFVAEDEREGILLIRKLLSYLPQNNLEDPPVIPCNDPIDRLDDSLNYFIP
                     DNPNKPYDVKTIIYSIIDENEFLEVQRHYAPNIVTGFSRFDGMTVGIVANQPNYLAGV
                     LDINASRKAARFVRFCDAFNIPLLTLVDVPGFLPGTAQEYGGIIIHGAKLLYAFGEAT
                     VPKVTIILRKAYGGAYDVMSSKHLRGDINYAWPTAEI"
     gene            complement(1308..1712)
                     /gene="mce"
                     /locus_tag="PK976_05105"
     CDS             complement(1308..1712)
                     /gene="mce"
                     /locus_tag="PK976_05105"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HRC67298.1"
                     /translation="MQISHIEHIGIAVRSIEESLPYYQEVLGLKCYGIEEVKDQKVRT
                     AFFQAGQTKLELLESTDPDGAIGKFIEKRGEGIHHIAFAVKEIESALEEARSKGIRLI
                     DESPRLGAEGLHIAFLHPKSTHGVLTELCENK"
BASE COUNT          510 a          428 c          321 g          522 t
ORIGIN      
        1 aatctcggct gtaggccagg cataattgat gtcgccacgc aggtgtttcg aactcatcac
       61 atcataagca cctccataag ccttgcgcaa aataatggta actttgggta cagtggcttc
      121 tccaaaagca tagagcaatt tggcaccatg aatgataatc cctccatatt cttgagccgt
      181 tccaggtaaa aaaccgggaa catccacaag agtaagtaat gggatattga aagcatcaca
      241 gaatctgacg aatcgggctg ccttcctcga ggcattgata tcgagcactc cggccagata
      301 attgggttga ttagccacaa tgccaacagt cattccatcg aaacgagaaa atccggttac
      361 gatgttgggt gcataatgcc gctgcacttc taaaaactca ttctcatcaa taatactata
      421 gataatggtt tttacatcat aaggcttatt gggattatcg ggaatgaagt agttgaggct
      481 gtcgtccaaa cgatcaattg gatcgttgca aggaatcacg ggagggtctt ctaaattgtt
      541 ctgcggtagg taactcaaca gttttcttat caatagtatg ccttcacgct catcttcagc
      601 aacaaaatgt gcaacccccg attttgcacc atgaatcatg gctcctccca attcctcctc
      661 agtaactacc tcaccagtga cagtctttac gacttttggc cctgttacga acatgtaact
      721 ggttttttga ctcatgatga tgaaatcggt aagagctggc gaatagacgg ctcctccagc
      781 acaaggacca aaaatagccg aaatttgagg tatgagaccc gaagcaagaa tatttcgttc
      841 gaaaatttca gcataacctg ccaggctcaa taccccttcc tgaatacggg ctccaccact
      901 gtcgttgagc ccgataatag gcgcccctac cttcatggcc tgatccatga tcttgcaaat
      961 cttttttgca tacgactccg ataaactacc accaaaaacc gtaaaatcct gcgaaaaaac
     1021 atacaccact cttccatcta ttgttccatg acccgtaaca actccatccg acaaataaag
     1081 ctgatcctgc atcccaaaat cggtacagcg atgtgttacg aacatgtcga attcttcaaa
     1141 actgccgtcg tcgagcaata agtctattct ttcacgtgcg gttagtttcc ccttggcatg
     1201 ctgagcatcg attctttttt gaccaccgcc taaactggct ttctgtcttt tttccagcaa
     1261 ttccaataat ttatcctcaa tagccatata aatgtataat tttattttta tttgttctca
     1321 cacaattcag tcaatactcc atgcgtagat ttaggatgca aaaaagcaat gtgcaatccc
     1381 tcggctccta atcgcggtga ttcgtctatg agccgaatcc ccttcgaccg agcctcttcg
     1441 agagcagatt ctatctcttt tactgcaaaa gcaatatgat ggattccttc tccccttttt
     1501 tcgatgaatt ttccaatggc accatcaggg tcagtcgatt caagaagttc gagtttggtc
     1561 tgaccggcct gaaaaaaagc tgttctaact ttctgatcct tcacctcttc tattccataa
     1621 cattttaaac ctaaaacttc ttgataatac ggtaaactct cttcgatgct tcgaacggca
     1681 atgccaatgt gctctatatg tgatatttgc atattaattt ggttttaaat tttcccttta
     1741 caaaggtaaa tttatttttt tgttaaaaaa acttaatgaa a
//
LOCUS       DAORPD010000379         1760 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_12758_length_1760_cov_2.256305, whole genome shotgun sequence.
ACCESSION   DAORPD010000379 DAORPD010000000
VERSION     DAORPD010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12758_length_1760_cov_2.256305"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="PK976_05110"
     CDS             <1..506
                     /locus_tag="PK976_05110"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HRC67299.1"
                     /translation="LRYFNPIGAHPSALIGELPLGIPSNLVPFITQTAIGKREVLRVY
                     GKDYNTPDGTAIRDYIHVVDLSEAHVVALDRMLQNKMKNNYEIFNLGTGQGHTVLEVI
                     RSFEKVSGVRLNYVFTDRRPGDVEKVWADTSYANQELGWKAKRSLDEMTLSAWRWELR
                     LAGKLNE"
     gene            530..1585
                     /gene="rfbB"
                     /locus_tag="PK976_05115"
     CDS             530..1585
                     /gene="rfbB"
                     /locus_tag="PK976_05115"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HRC67300.1"
                     /translation="MEKKILITGGAGFIGSHVVRLFVNKYPQYHIFNLDKLTYAGNLE
                     NLRDIEDKPNYSFIRADIQDNDAIMKLFVLHRFDGVIHLAAESHVDRSISNPLEFVMT
                     NVVGTVNLLNAARFIWKGNFEDKRFYHISTDEVYGSLGSIGLFTEETPYEPHSPYSAS
                     KASSDHFVRAYHDTYGLPTVISNCSNNYGAYQFPEKLIPLCINNIRFNLTLPVYGKGE
                     NIRDWLWVEDHAAAIDLIFHRAPNGETYNIGGNNEWKNIDLIRLLCRIMDRKLERPSG
                     TSEKLITFVKDRAGHDLRYAIDSSKLQEQLGWKPSVTFEEGLEKTIDWYLSNPEWLAH
                     VTSGEYQKYYEEQYIKR"
BASE COUNT          518 a          347 c          376 g          519 t
ORIGIN      
        1 ccctgcgata tttcaatccc attggggccc atccatcggc actgattggg gaattacctt
       61 tgggtattcc cagtaatttg gtgcctttta tcacgcaaac cgctattgga aaacgtgaag
      121 ttttgcgtgt ttatggaaaa gactacaata cacccgatgg cacggccatt cgcgactata
      181 ttcacgtggt cgatctttcc gaagcccatg tggtagccct cgaccgtatg ctccaaaata
      241 aaatgaagaa taactacgaa atctttaatc tgggtacagg gcagggacat acagtgttag
      301 aagtcattcg gtcattcgaa aaagtgtcgg gtgttagatt gaattatgtt tttaccgatc
      361 gtcggccagg tgatgtggaa aaagtgtggg ctgatacttc ttatgcaaat caggaattag
      421 gatggaaggc taaacgttcc ctcgacgaaa tgactctctc agcctggcgt tgggaacttc
      481 gactggccgg aaaattaaat gaataacatt attattgaat tgtttggcta tggaaaagaa
      541 aatacttatt acgggtggag ctgggtttat tggttcgcat gtagtgaggc tttttgtgaa
      601 taaatatcct caatatcata tttttaacct cgataaactt acctatgctg gaaacctcga
      661 aaatctgcgc gatatcgaag ataaacctaa ctattccttt atcagggcgg atattcaaga
      721 caatgacgct ataatgaaac tatttgtgct tcatcggttc gatggggtca tccatttggc
      781 cgccgagtct catgtagacc gttctatctc caatcccctc gaatttgtca tgaccaatgt
      841 ggtgggtacg gtcaatctat tgaatgctgc acgttttatc tggaaaggaa atttcgaaga
      901 caaacgattt tatcatattt caaccgatga ggtctatggt tctttaggtt caataggact
      961 tttcaccgaa gaaactccct atgagcccca ttccccttat tcagccagca aagccagcag
     1021 tgatcatttt gtgagagctt accacgatac ttatggtttg ccaacggtta tttcgaattg
     1081 ttcgaataat tatggcgctt atcagtttcc tgaaaaactt attcctctgt gcatcaataa
     1141 tatacgtttc aatttgacct tgccagttta tgggaaaggc gaaaacataa gagattggtt
     1201 atgggttgaa gaccatgcgg cagctatcga tttgattttt catcgagcac ctaatggcga
     1261 aacctataac ataggcggaa ataatgaatg gaagaacatc gacttgataa gacttttatg
     1321 cagaataatg gatcgtaagc tggaaaggcc ttccggaacc tcggaaaagc tgattacttt
     1381 tgttaaagac agagctggcc atgacctgcg ctatgccatc gactcatcca agttgcagga
     1441 gcaattagga tggaaaccca gcgtaacttt cgaggaaggc ctcgaaaaaa ccatagattg
     1501 gtatctctcc aatcctgaat ggcttgctca tgtaacttct ggagaatatc aaaaatacta
     1561 tgaagagcag tatataaaac gataaaacct gctcccctca tattcgagtt tttcttttga
     1621 aagggttaaa ttctctattt aatatggcaa ctggccaata ttttccttaa aatttggaga
     1681 aagtttcgac tatttttcta tttaaaaata gaagcgattt tggagaagcc ctccttaaac
     1741 gaatccatgg ccgatgaaaa
//
LOCUS       DAORPD010000380         1758 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_12778_length_1758_cov_2.329419, whole genome shotgun sequence.
ACCESSION   DAORPD010000380 DAORPD010000000
VERSION     DAORPD010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12778_length_1758_cov_2.329419"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1045..>1758
                     /locus_tag="PK976_05120"
     CDS             1045..>1758
                     /locus_tag="PK976_05120"
                     /inference="COORDINATES: protein motif:HMM:NF012879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRC67301.1"
                     /translation="MKRTLFLTGFLLMGFWLNAQFQYQPKASVPTDPSVKIGQLPNRL
                     TYYIQKNGQPEKRAEFYLIVNAGAILEDVDQNGLAHFCEHMAFNGSKHFQKHQIINYL
                     QSIGMKFGPEINAFTSHDVTAYMLQNVPLNDPAQMDTALLILYDWASQVSFEDAEIDA
                     ERGVIHEEWRTGRNAMERMNKQAMKKLFYNSKYAVHDVIGDIQIIDIFPYETVRRFYH
                     DWYRPDLQAVIAVGDFDPQI"
BASE COUNT          574 a          310 c          330 g          544 t
ORIGIN      
        1 gaatttacat gatgccattt tacaggaaag aatttgtgtt ccccgaaagt tgaaagttaa
       61 aaattcaact tgctgtatac aaggaaattg catctctaag gctctcaagc tgggttaatg
      121 ttttcgagct taattgggtt ttcataggaa acggttttag tttttattga caataagcaa
      181 gttggataat gttttggaag atttttcatc gcagacccta ataagataaa tgccgggaat
      241 gagatgattg ggaaggtgca aacttttttt tgttcgactc atcgaaagga acccaaacga
      301 tttgccctac taagggagac accgctcccc aaagcaaaca aaaaattaaa ccaattacat
      361 tttcttcata taaataattg aatttaagct aaatacattg gtggaataca ttcatctcca
      421 tcaacatcaa atataatact ttaataagta aaatttcaaa gccgcagtta aaatattttt
      481 tcttggagga gaactcatta caaggagcaa acgaaaattc tgcctattca gagaataata
      541 cacagcagat agataagctg gcgattaggg aaaaattttt taagatgaaa ttcgaataac
      601 tgtaaatcag gtttaatgat tgttcaaacc cctaaatccg gaaaagatat gcttcttctt
      661 gaatacttat atggattacc atttagcatc attatactga attatatttc atacggatgc
      721 cgctcaaact tcattcttta aatttttaag aatatttttt catagaatgg attaacaaca
      781 atggattaga cttgcaattt agatttcaat taatatctca ggttgttaaa tcattttata
      841 gagagagcaa taaaaaaagc actgcgatgt gcttcattcc attgttttat tgctgattta
      901 aagtctttcg ttatcaagaa aggaaaggaa taggttattt tggtagtgta tagtttaatt
      961 gtttatatat ttgtcctcaa aatgcaatgc taataaggag atattttaat agatgacata
     1021 ccaattagtt aaatataaaa atttatgaaa cgcactttat ttctcacagg cttcttattg
     1081 atgggctttt ggcttaatgc ccagtttcag tatcaaccca aggcatcagt gcctaccgat
     1141 ccttcggtaa aaatcgggca acttcccaat aggctgactt attatattca aaagaatggc
     1201 cagcccgaaa agagagccga attttatctg attgtgaatg ctggagccat tctcgaagat
     1261 gtcgatcaga acggattggc tcatttttgc gagcacatgg ccttcaatgg cagcaaacat
     1321 tttcagaagc atcagattat aaattattta caatccatag gcatgaaatt cgggcccgag
     1381 atcaatgcct ttacaagcca cgatgttact gcctatatgc ttcagaatgt tcccctcaac
     1441 gaccctgctc agatggatac agccttgctg attttatacg attgggcttc gcaggttagc
     1501 ttcgaggatg cagagattga tgccgaacga ggagtaattc atgaggaatg gcgtacgggc
     1561 cgcaatgcca tggaacgcat gaataagcaa gccatgaaaa aactcttcta caactcgaaa
     1621 tatgcggttc acgacgtgat tggtgatatc caaatcattg acattttccc ttatgaaacc
     1681 gtcaggagat tttatcacga ctggtatcgt cccgatcttc aggccgtaat tgcggtggga
     1741 gatttcgatc ctcaaatc
//
LOCUS       DAORPD010000381         1757 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_12788_length_1757_cov_2.293772, whole genome shotgun sequence.
ACCESSION   DAORPD010000381 DAORPD010000000
VERSION     DAORPD010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12788_length_1757_cov_2.293772"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..995)
                     /gene="ppk2"
                     /locus_tag="PK976_05125"
     CDS             complement(90..995)
                     /gene="ppk2"
                     /locus_tag="PK976_05125"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF015909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HRC67302.1"
                     /translation="MGNNSNCNELNELFGEEFRKYIQPRDESEQIKFGEDADNGHEVS
                     KLGKKFYEKELRKLQIELVKLQEWVKAKGLRVVVIFEGRDAAGKGGVIKRITECLNPR
                     ICRIAALPVPTEKERTQWYFQRYVSYLPSAGEMVLFDRSWYNRAGVERVMGFCTEEEY
                     WEFFRSCPLFEEMLIRSGIILIKYWFSVSDHEQEQRFQQRLADPTKRWKLSPMDIASR
                     EKWEDYSKAKDEMFAFTDTKISPWWDVNADDKRRARLNCISHLLSMIPYEELPSPLIK
                     FSPRKESTGYVRPPIKRQNFVPERY"
     gene            complement(1013..>1757)
                     /locus_tag="PK976_05130"
     CDS             complement(1013..>1757)
                     /locus_tag="PK976_05130"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HRC67303.1"
                     /translation="IRVIKGKLLFLEDHLKRFENSARNMGFQLSLSQKEITYRLYEML
                     HANGSLDGNIELDVNYRPDSFSPKQTFVAWYIPHHYPTEEEYRQGVKTGLFYYERKDP
                     NIKYRNIDLKEKVKIFIEKNKIYESLLVDDGGAVTEGSKSNVFFIKENQFFTPPLSKV
                     LPGVTRQKIIYLINQQNIVLNEGNIYVKTLNTYEASFLTGTSPKVLPIAFINEQSYNV
                     NHPLLHFIMKSYDDIIESYLNEGINGLNQ"
BASE COUNT          519 a          360 c          292 g          586 t
ORIGIN      
        1 gccagaagtt gctttacggg atttgcaaca atcaaactca ataaaaacat aaaaattaat
       61 aactttttca ttttcattgc aaaaaattct taatatcgtt ctggtacaaa attttgcctc
      121 tttattggag gtctaacata acccgttgat tcttttcgag gtgaaaattt gatgaggggt
      181 gaaggtaatt cttcataagg gatcatgctt agtaaatgag agatgcaatt gagacgggct
      241 ctgcgtttat catcggcatt cacgtcccac cagggagata ttttcgtgtc ggtaaaggca
      301 aacatctcat cttttgcttt gctataatct tcccattttt cgcgcgaggc gatgtccatt
      361 ggactgagtt tccaacgttt agtcggatcg gcaagacgtt gctgaaacct ttgctcctgt
      421 tcatgatcac ttaccgaaaa ccaatattta ataagaataa ttccactacg aatgagcatt
      481 tcttcgaaaa gagggcatga acggaaaaat tcccaatact cttcttcagt acaaaaaccc
      541 atcacacgtt ccacacctgc cctgttgtac catgagcgat cgaaaagaac catctcgccc
      601 gcactgggca aataagaaac atatctctga aaataccact gtgtgcgttc cttttcggtt
      661 ggaactggca aggctgcaat tcgacaaata cgtggattta gacattcggt aatacgcttg
      721 ataacgccac cctttcctgc tgcatcacga ccttcgaaaa taaccactac cctcaatccc
      781 ttcgccttta cccattcctg taatttgacc agttctatct gtaatttccg taattctttt
      841 tcatagaatt tctttcctaa tttcgaaact tcatggccat tatcagcgtc ttctccaaat
      901 tttatttgtt cagattcatc tcgaggctga atatatttcc gaaattcttc cccaaacaat
      961 tcattcaatt cattacagtt ggaattgttt cccataaatt attgatttta aatcattgat
     1021 tcaaaccatt tatcccctca ttgagataac tttcgatgat gtcgtcataa gatttcataa
     1081 tgaaatgcaa aagcggatga tttacattat atgattgttc attgataaat gcaatgggta
     1141 gtacttttgg tgaagttcca gttaaaaaag aagcctcata agtattcagc gtttttacat
     1201 aaatatttcc ctcatttaaa acaatatttt gttgattgat taggtaaatg attttttgac
     1261 gagtaacacc aggcagtact ttcgaaagtg gtggcgtaaa aaattgattt tcttttataa
     1321 agaacacatt cgacttgctc ccttctgtaa ctgctccgcc atcatccaca agtaaagatt
     1381 catatatttt attcttttcg ataaaaattt ttaccttttc cttcaaatct atatttctat
     1441 acttgatatt gggatccttt cgttcataat agaacagccc ggtttttact ccttgacggt
     1501 attcttcttc cgtagggtaa tggtgaggaa tataccatgc tacgaaagtt tgttttgggg
     1561 aaaaactatc aggccggtaa ttcacatcga gttcaatatt gccatcgagg gaaccattgg
     1621 catgcaacat ttcataaagt cgatatgtaa tttccttttg gctcaatgaa agctgaaatc
     1681 ccatgtttcg agcagaattc tcgaatcttt tcaaatgatc ttccagaaat agaagtttgc
     1741 ctttaataac cctgatg
//
LOCUS       DAORPD010000382         1756 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_12799_length_1756_cov_2.144621, whole genome shotgun sequence.
ACCESSION   DAORPD010000382 DAORPD010000000
VERSION     DAORPD010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12799_length_1756_cov_2.144621"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1137
                     /locus_tag="PK976_05135"
     CDS             <1..1137
                     /locus_tag="PK976_05135"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF003705.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HRC67304.1"
                     /translation="HCHFVGNDNSPKVFFAPGRINLIGEHTDYNGGFVMPLAFQLGTY
                     IVTAPSANKCFRFYSENFKSHYEHPYGLETSKGKADWFKYPMGVVDRFVRHSGWPDCG
                     WDLYFFGDLPLSAGLSSSASIEMVTAVAMDFIFQSTYPMIELVKIAKEAENEFVGLQC
                     GILDMFASGMGRKGHCLLIDCNSMEFSTIPFKTGDYRLIIMNTNKRRGLADSKYNERV
                     KECEQALADLRQEVDIDFLCALSYSDFQKYGAAINNPMHYKRARHVISENQRTQDAAQ
                     ALIANDIRRFGKLMVESHKSLHSDYEVTGLELDTLVEASMEAEGVMGARMTGAGFGGC
                     AIALVHRNHISKFQHHVEKVYLQKTGLKVEFYDAYAVNGACRIL"
     gene            1205..>1756
                     /locus_tag="PK976_05140"
     CDS             1205..>1756
                     /locus_tag="PK976_05140"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HRC67305.1"
                     /translation="MKLHHNILAFWIFLFITLAIFQSNAIAQNIPDKKIPDTYCLNED
                     ELQLFNLLNRERQQKGLPKLELSKSLSYVAYLHVRDLYINRPDTVKGCNMHSWSSKGQ
                     WTPFCYPRDQTKRRSIWDKPKEITLYPGSAFEVVYYSEDKTGPVEIIELWKGVTPSAA
                     LIFNTGKYMKNHWKSAGVSIYKGY"
BASE COUNT          524 a          337 c          373 g          522 t
ORIGIN      
        1 cactgtcatt ttgtggggaa tgacaattcg cctaaggtat tttttgctcc gggtcgaatc
       61 aatctcatag gtgaacatac cgattataat ggcggctttg ttatgccatt ggcttttcaa
      121 ctgggaacat atattgtaac agccccaagt gcgaacaaat gtttccggtt ttattcggag
      181 aatttcaaga gccattatga gcatccttat ggtttagaaa cctcaaaagg caaggcagat
      241 tggtttaagt atcccatggg agtggttgac aggttcgtaa ggcactctgg atggccagat
      301 tgcggatggg atttgtattt cttcggtgat ttgcctttgt ctgccggttt gtcttcctca
      361 gcttccatcg aaatggttac agccgtcgcc atggatttta tcttccaatc aacctatccc
      421 atgatcgagc tcgttaaaat tgctaaagaa gccgaaaatg aatttgtagg tcttcaatgt
      481 ggtattctcg acatgtttgc ttcaggaatg ggtcgaaaag gccattgtct tttgatcgac
      541 tgcaattcga tggagttttc caccattcct ttcaaaacgg gtgattatcg gttaattatc
      601 atgaacacca ataaaagaag aggattggcc gattcgaaat acaacgagag ggtaaaagaa
      661 tgcgagcagg cacttgccga cttacggcaa gaagtagata tagattttct ttgcgcctta
      721 tcttattctg attttcaaaa gtatggggca gctatcaaca atcccatgca ttacaaaaga
      781 gccagacatg tgatctccga aaatcaacgt acgcaagatg ctgctcaggc cttgatagct
      841 aatgatattc gccgatttgg gaaactaatg gttgaatcac acaaaagcct tcattctgat
      901 tatgaggtta ctggccttga actcgatacc ctcgtagaag cttccatgga ggccgaagga
      961 gtgatgggtg ccagaatgac cggcgcagga tttggaggat gtgctattgc tctcgttcat
     1021 cgaaatcata tatctaaatt tcaacatcat gtcgaaaaag tatatttgca aaaaaccgga
     1081 ttaaaagtcg aattttatga tgcttatgcc gtaaatggtg catgccgtat tttataaaaa
     1141 aatgcctaac ctttcatctc taagaaatat gtctggaaaa tattggattt tattggcaaa
     1201 gtttatgaaa cttcatcaca acattctggc gttttggatt tttcttttca ttacccttgc
     1261 aatatttcag agtaatgcta tcgcacagaa catacctgat aaaaaaatcc ctgatactta
     1321 ttgtttgaat gaagatgaat tacagctatt taacctgttg aatcgggaac gacagcaaaa
     1381 aggacttccc aaattggaat tatccaaatc cttgtcctat gtagcctatc ttcatgtcag
     1441 agatctgtac ataaaccgtc ccgataccgt gaaaggatgt aatatgcata gctggagttc
     1501 aaaaggccaa tggacgccat tttgttaccc gagagatcaa accaagcgac gatcgatatg
     1561 ggataaaccc aaagaaataa ccctatatcc tgggtcagct ttcgaagtag tttattattc
     1621 cgaagacaaa actggtccgg ttgaaattat tgaactatgg aaaggagtaa ctccttctgc
     1681 tgctctgata ttcaatactg gaaaatacat gaagaaccac tggaaatcgg ctggtgtgtc
     1741 tatatataag ggttat
//
LOCUS       DAORPD010000383         1754 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_12821_length_1754_cov_2.085344, whole genome shotgun sequence.
ACCESSION   DAORPD010000383 DAORPD010000000
VERSION     DAORPD010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12821_length_1754_cov_2.085344"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="PK976_05145"
     CDS             complement(<1..315)
                     /locus_tag="PK976_05145"
                     /inference="COORDINATES: protein motif:HMM:NF024959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRC67306.1"
                     /translation="MSNLGKLERKRNEGIVKQASMLLDVTQKLIMKAEERNPSFMPMF
                     RGNYSRTGIAKTKSINQYPKVLYTFHELAGVYASPVMAEGKVYFGSDSHSFFCLDAQD
                     GSL"
     gene            complement(278..1216)
                     /locus_tag="PK976_05150"
     CDS             complement(278..1216)
                     /locus_tag="PK976_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67307.1"
                     /translation="MHRRLRTILHLLEQRKLTELQLEFDAILLEDHSEEVKYLYHLFR
                     QKLFTELTHEIQDVLQKTQHIVPSDINIQGIKTQIRILETQLSVVQYKKTEILKEIDR
                     FRIRHNSDLGWLIGKILKIQMEIFYLLQDVDSSYQANYQEAKKDYTAYQHQRRSLEKI
                     QPLQILDEKQRESMKKMYREASKLCHPDIVNDDFREEARKVFIELNRAYMINDIQKVE
                     EIHKLLTRSRMFSRLRTPSEERELLEARLARLQADLKILQSEIRELEESQVYHTLMNI
                     RDWDEYFEQTKMKLNAEYQFLKTEHEQLRKVRTEKK"
     gene            complement(1226..>1754)
                     /locus_tag="PK976_05155"
     CDS             complement(1226..>1754)
                     /locus_tag="PK976_05155"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRC67308.1"
                     /translation="KNDRRWRIEHAQVVDPEDLMKFGQFSIIPSVQPTHATSDMDWAE
                     KRLGPQRLQYAYTYHDLLTQNGWIALGTDFPIEEVNPIFTFYAATTRKNLQHRPPGGF
                     LPQNKLSRRETLYGMTLWAAKSTFLENEIGSIEPGKKADFIVTSDDLMQLPDDSIPHI
                     VVEQTWINGKCVFHK"
BASE COUNT          465 a          354 c          365 g          570 t
ORIGIN      
        1 gagcgaacca tcctgcgcat ctaaacagaa aaaactatgg ctgtcggagc cgaaatatac
       61 tttcccttcg gccatcaccg gactggcata aacgccggca agttcatgaa aggtataaag
      121 gacttttgga tattgattta tagactttgt tttggcaatt cctgttctgg aataattacc
      181 gcggaacatg ggcataaacg aaggattcct ttcttcggcc ttcatgatga gcttctgcgt
      241 tacatccaac aacatgctgg cttgttttac gattccttca tttcttttcc gttctaactt
      301 tcctaagttg ctcatgttcg gttttcaaaa attgatattc agcattcaac ttcatttttg
      361 tctgctcaaa atattcatcc caatcgcgaa tattcatcag agtatgataa acttgacttt
      421 cttcgagttc acggatttcg gactgcaaaa tttttaaatc ggcctgcagg cgtgctaaac
      481 gagcctccag aagttccctt tcttcactag gagtacgtag cctactaaac atcctgctcc
      541 gtgtaagtaa tttatgtatc tcttctactt tttgaatgtc gttaatcata taggcgcgat
      601 tgagttctat gaaaaccttt cgagcttctt cacgaaagtc gtcgttcacg atatcggggt
      661 gacacagttt actggcttca cgatacattt ttttcatgct ttcgcgctgc ttttcatcaa
      721 gaatttgtaa aggctgaatt ttttccaagc tgcgcctttg atgctgataa gcagtataat
      781 cctttttggc ttcttggtaa tttgcctgat atgaggaatc tacatcctga agtaaataaa
      841 atatctccat ctgtattttc aaaatctttc cgataagcca tcccaagtca ctgttatgac
      901 gaatacgaaa ccgatcaatc tccttgagta tttctgtttt cttatattgg accaccgaaa
      961 gctgggtttc aagaattctt atttgagttt taattccttg aatgttaatg tcggaaggaa
     1021 caatatgctg ggttttttgc aatacatctt gaatctcatg ggtcaattct gtgaatagct
     1081 tttgacgaaa gagatggtaa agatatttca cttcttcgct atgatcttcg aggagaatgg
     1141 catcaaactc gagctggagt tcggtcaatt tgcgctgttc caataaatgt aaaatggttc
     1201 gcagacggcg gtgcatcagc agctgttatt tatgaaaaac acatttacca ttaatccaag
     1261 tttgttctac cacaatatga ggtatgctgt cgtcgggaag ctgcatcaaa tcatcggaag
     1321 ttacgataaa atcggctttt ttacctggtt ctatcgagcc gatttcattt tcgaggaatg
     1381 ttgattttgc agcccaaagc gtcatgccat acaaggtttc gcgtcgtgag agtttgtttt
     1441 ggggtaaaaa accgcctgga ggtcgatgtt gcaaattttt tcgggtagtg gctgcataaa
     1501 aggtgaatat gggatttacc tcttctatgg ggaaatcggt ccccaaagct atccatccgt
     1561 tttgggtgag aagatcgtgg taagtataag catattggag gcgttggggt ccaaggcgtt
     1621 tttcagccca atccatatcg gaagttgcgt gagttggctg aacggaagga atgatggaaa
     1681 attgaccaaa tttcatcaaa tcttcaggat cgactacctg agcatgctct atacgccagc
     1741 gacgatcatt ctta
//
LOCUS       DAORPD010000384         1746 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_12918_length_1746_cov_2.496747, whole genome shotgun sequence.
ACCESSION   DAORPD010000384 DAORPD010000000
VERSION     DAORPD010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12918_length_1746_cov_2.496747"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="PK976_05160"
     CDS             <1..198
                     /locus_tag="PK976_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009436957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase"
                     /protein_id="HRC67309.1"
                     /translation="EFPEVDFRIVCSKGTYIRAIARDFGEYLGCGAYLKSLRRTRIGN
                     FDVKDAFDIETFKSSLYHKLE"
     gene            211..>1746
                     /gene="gldG"
                     /locus_tag="PK976_05165"
     CDS             211..>1746
                     /gene="gldG"
                     /locus_tag="PK976_05165"
                     /inference="COORDINATES: protein motif:HMM:TIGR03521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     substrate-binding protein GldG"
                     /protein_id="HRC67310.1"
                     /translation="MSLALRPKLLEFFKFAIRFCFMNAPPKDNTTSSSRKKIARQAIS
                     KLLVGILIVILVNIIGSYSFTRFDLTSEKRYTLSESTKKLIKEVDDYVYFRIYLEGDF
                     PAGFKRLRNETREMLDEMRAYNKFINYEFINPNASSNPKDRQNIYRQLIEKGLNPTDL
                     QVKDKEGESRQIIFPGAIASYRGKETPVQLLTGQMGARPEEILNLSVENLEYNLANTI
                     RKLIVSYKPKVAFIYGHGELEEAFIYDISQSMAEYYTVERIKLDGQINALTEHKTDSG
                     RISIFNKYKAIIIAKPDSAFTEKDKFIIDQFLMRGGKILWFIDGVNAEMDSLQNKSST
                     LGLAHDINLEDMLFRYGVRINNDLVLDANCLPIMMVTGTIGNQPRFSFVPWIFSPLAN
                     PATKHPIVNNLNPIKTQFISSIDTIAIKGVKKTIMLSSGQYSRKIIAPIIIDLNIARQ
                     EPDLKLFNQPYIPLAVFLDGKFPSLYEHRIPANISDSPEIGFHAESKPTQMLVVSDGD
                     VIRN"
BASE COUNT          554 a          365 c          335 g          492 t
ORIGIN      
        1 gaatttcccg aagtcgactt caggatcgtt tgtagtaaag gaacctatat acgtgctata
       61 gcacgcgatt ttggcgaata tttgggatgt ggagcctatc tgaaaagtct gcgccgtacc
      121 cgaatcggaa atttcgatgt aaaagatgcc ttcgatatcg aaaccttcaa atcatcgctc
      181 taccataagt tagaataagt ctatcgatga ttgagtcttg cacttcggcc taaactactg
      241 gaatttttta aatttgccat tcgtttttgt tttatgaacg ctccacctaa agacaataca
      301 accagcagtt cgagaaagaa aattgcacgg caggcaattt cgaagctctt agtgggaatt
      361 ctcatcgtta tccttgtcaa catcattggt tcttattcat ttacgcgctt cgatctaact
      421 tcggaaaaac gttacacact ttccgaatct actaaaaagc ttataaaaga agtagatgat
      481 tacgtttatt ttagaattta tctggaaggc gattttccag ctggattcaa gcgattgaga
      541 aacgaaactc gtgagatgct cgatgaaatg agggcttata acaaattcat taattatgag
      601 tttattaatc ctaatgcaag tagtaatccc aaagaccggc aaaacatata caggcaattg
      661 atcgagaaag gacttaatcc taccgattta caagttaaag ataaggaagg cgaaagtcgg
      721 cagatcattt tcccaggtgc cattgcctca tatcggggga aagaaactcc cgttcagctc
      781 cttacgggtc aaatgggggc aaggcctgaa gaaatcttga atctctcggt cgaaaacctg
      841 gaatataatt tagccaatac catacgaaaa ctcattgtat cctataaacc gaaagttgcc
      901 tttatctatg gtcatggcga actcgaagag gcttttatct acgatatttc tcaaagcatg
      961 gccgaatatt acaccgtcga aagaatcaaa ctcgatggcc agatcaatgc acttaccgaa
     1021 cacaaaaccg attcaggacg tatttccatt ttcaataaat ataaggccat catcatagcc
     1081 aagcccgact ctgcattcac cgaaaaagac aagtttatca tcgatcaatt tttgatgcga
     1141 ggtggaaaaa ttctgtggtt tatagatgga gtaaatgcag aaatggacag cctccagaac
     1201 aaaagcagta ctttgggctt ggcacacgac ataaatctcg aggatatgct ttttcgctat
     1261 ggagtacgta tcaataatga tcttgtttta gatgccaact gccttccgat aatgatggtt
     1321 acaggaacta ttggcaatca gcctcgcttt agctttgtac catggatttt ttccccattg
     1381 gctaatccag ctacaaaaca tcccatcgtt aacaacctaa atcctattaa aacacagttt
     1441 attagcagta tagatacaat agccatcaag ggagttaaaa aaaccatcat gctttcgagc
     1501 gggcaatatt cgcgtaaaat tattgcgccg attatcattg atctcaatat agcccgtcaa
     1561 gaacccgacc tgaagttgtt caatcaacct tatattcctt tggccgtgtt tctcgatgga
     1621 aaatttcctt cattatatga acatcgtatc ccagctaata ttagcgatag ccccgaaata
     1681 ggattccatg ccgaaagcaa accaactcaa atgcttgtgg tgagcgatgg agacgttatc
     1741 cgtaac
//
LOCUS       DAORPD010000385         1745 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_12933_length_1745_cov_2.436686, whole genome shotgun sequence.
ACCESSION   DAORPD010000385 DAORPD010000000
VERSION     DAORPD010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12933_length_1745_cov_2.436686"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            315..1007
                     /gene="mtgA"
                     /locus_tag="PK976_05170"
     CDS             315..1007
                     /gene="mtgA"
                     /locus_tag="PK976_05170"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009138130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HRC67311.1"
                     /translation="MKKLMRRVLKWIRNIILILIGLSLFFTLLYKFVPVYYTPLMFIR
                     YFENVKAGKEARIERNWKRLDEISPNLWLAVVASEDNNFLKHHGFDMDAIKKAQKHNQ
                     THARKRGASTISQQVAKNVFLWPSRTWVRKGLETYFTFLIETFWSKKRILEVYLNVIE
                     IGPGIYGAEAASQIYFHKPASKLTRGEAALIAAVLPAPLRRNPAKPSPYVLSRQQWIL
                     WNMNNIGKIEFD"
     gene            1018..>1745
                     /locus_tag="PK976_05175"
     CDS             1018..>1745
                     /locus_tag="PK976_05175"
                     /inference="COORDINATES: protein motif:HMM:TIGR01533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HRC67312.1"
                     /translation="MMKKFLIVLLCLVMAACQVTKKSSIQGHDDNDHLIMATLWFQQA
                     AEVRALYYQAFHLAQLQLEHDLAFGDNSTKRAVVLDIDETVLDNSPHEGYLIQNGKSY
                     PEGWSEWVSLAQAAALPGALDFINFAISHDVDVFYITNRKKDELNVTILNLRRLGFSM
                     ADSSHILVRTDGANKENRRQRVRQTHRIVLLIGDNLNDFSNLWEKTLPQRRNFLADSL
                     QYEFGKRFIILPNPMYGDWENALY"
BASE COUNT          510 a          325 c          358 g          552 t
ORIGIN      
        1 ttccccaaaa tatattttca caggattgta aaaaacgaaa tttttcattg tgtgggtttt
       61 tcgatttcaa tttttcgatg taaagttatt attcactatg ttagttgcaa aaataatagg
      121 atttcatcga gagaattatt ttaattgtga ttcgattgat cgatcgaggt gtagatagca
      181 tatcttcctt aatcaatcat ttttcatgac atcagctgaa tcttaggcta tcctccttaa
      241 ttttgtgtaa tcatttcttt ttatcttttt tgcacgaaag atgactttcg gtcagattca
      301 gcttgtttgc ccctatgaaa aaattgatga gacgtgtttt aaagtggatc aggaatatta
      361 ttctaattct tattggtttg tctttatttt tcactctact ctacaagttt gttcccgtat
      421 attatactcc attgatgttt attaggtatt tcgagaatgt caaggcagga aaggaagcaa
      481 gaatagaaag gaattggaaa agactggatg agatctcgcc caatttatgg ttggcggtgg
      541 tagcttcgga agacaataat tttttgaagc atcatggatt cgacatggat gccataaaaa
      601 aggcccaaaa acacaatcag acgcatgcca gaaaaagagg agcaagtacc attagccaac
      661 aggtagctaa gaacgttttt ttatggccat ctcgtacttg ggttcgaaag ggattggaga
      721 catatttcac ctttctgata gaaacttttt ggagcaaaaa acgaattctc gaagtttatt
      781 tgaatgtaat agagataggc cctgggattt atggggcaga agcagccagc cagatttact
      841 ttcacaagcc tgcttcaaaa cttacacgtg gagaggctgc acttattgcg gcagtgttgc
      901 cggctcctct caggcgaaat cccgctaaac catctcctta tgtactttca cgtcaacaat
      961 ggattctttg gaatatgaat aatatcggta aaattgaatt cgattgaatt tacttatatg
     1021 atgaaaaaat ttctaatagt tttattatgt ttagtcatgg ccgcctgtca ggttaccaaa
     1081 aaatcgtcaa ttcagggaca cgacgataat gaccatttga ttatggcgac gctttggttt
     1141 cagcaagcgg ccgaagttcg tgctttatat tatcaggctt ttcatttggc tcagctccag
     1201 ctcgagcacg accttgcctt tggagataac tcaacaaaaa gagccgtggt actcgatatt
     1261 gatgaaaccg tgctcgataa tagccctcat gaaggttatt tgattcaaaa cgggaaaagt
     1321 tatccagaag gatggagtga atgggtttct ctggcacagg ccgccgctct tccaggtgca
     1381 ctcgacttta tcaattttgc cataagccat gatgttgatg ttttttatat cactaaccgt
     1441 aaaaaagatg aactgaatgt taccatcctc aatcttcggc gattagggtt ctctatggcc
     1501 gattcttcgc acatactcgt aagaaccgac ggggctaaca aggagaatcg ccggcagaga
     1561 gttcgtcaaa ctcatcgaat cgttttattg attggggata atttgaacga ttttagtaat
     1621 ttatgggaaa aaactttgcc ccaacgtcgc aacttccttg ccgattcact gcaatatgag
     1681 tttggcaagc gcttcatcat tttacccaat cctatgtacg gagactggga aaacgcactc
     1741 tatca
//
LOCUS       DAORPD010000386         1745 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_12937_length_1745_cov_2.018935, whole genome shotgun sequence.
ACCESSION   DAORPD010000386 DAORPD010000000
VERSION     DAORPD010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12937_length_1745_cov_2.018935"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..269)
                     /locus_tag="PK976_05180"
     CDS             complement(111..269)
                     /locus_tag="PK976_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67313.1"
                     /translation="MRKEKMESGFNEIVDEEIKVIQKDSDTDYGYIKMTYDLSCLKIL
                     INIKKTYS"
     gene            complement(697..1293)
                     /locus_tag="PK976_05185"
     CDS             complement(697..1293)
                     /locus_tag="PK976_05185"
                     /inference="COORDINATES: protein motif:HMM:NF003191.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HRC67314.1"
                     /translation="MIEWFEAHPGFYTYAIIPFLIFFARICDQSIGTLRLILISKGYK
                     TIAPFIGFFEVIIWVLAISQIFKHLDNIVCYLAYGAGFATGNYIGILLEERISLGTAL
                     IRIIPRYDTTDLITYLSERGYGLTFWDAEGRYGNVKIILTIIKRKEIPQVVDIINRFN
                     PNAFYTIEEIKAVNKGILKDEKATDIFRNLIFGPRKAK"
     gene            complement(1306..>1745)
                     /locus_tag="PK976_05190"
     CDS             complement(1306..>1745)
                     /locus_tag="PK976_05190"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRC67315.1"
                     /translation="YLFYSDHFDLEGNHDYDHPFLKDVPMQAMKIGESVVLRNVFFAT
                     DSYELMPESRAELDRLAALLMQNPNLQIEIAGHTDSQGNDDYNMELSLKRARAVYSYL
                     ISKNIDPLRLTYKGYGETKPVDTNDTPEGRANNRRTEFKIIHL"
BASE COUNT          483 a          329 c          343 g          590 t
ORIGIN      
        1 aggagctaat gatgcctttt tttatgttgc tcttcatacg ttgtttctat cggcttttcc
       61 ccccgataat tattcttgca cctggttatg aagcaattgg ttttcgacca ttagctataa
      121 gtttttttta tgttaataag aatcttcaag catgataaat cataggtcat cttgatgtat
      181 ccataatcag tatcagaatc tttctggatg acttttattt cttcatccac tatttcattg
      241 aatccacttt ccattttttc ttttctcaca gcaagtttgg tttcaattgg caggatcaac
      301 cctgctcagg gaggctgttt ttttatgatt ttagacttct tctttattgt gaattttttt
      361 gggtattaaa gttcagattt ctatccatgc ttttatccaa tattcaggga gcaaatccaa
      421 ataaggaaga ttagtgataa taaaaattcc aaagaatcgg atatatgaag gtgtggggct
      481 ataaaatatg gttatctttg agaaaaaatt taaaaatgat aagtgatgca gtgtccatgt
      541 gaaggggaag atggggcgat acttcctgcc tattccttat ttcacaatca ccaaatatta
      601 attttttctt tctcttctga ctctggtttc tattttcccc attcatcaaa atcccctata
      661 cctcatagcc cttaacgtgt atctcgcata gggtcatcat tttgctttac gcggcccaaa
      721 aattagattg cgaaaaatgt cagttgcctt ttcatccttt agaatgcctt tattgacggc
      781 ttttatttct tcaattgtat aaaatgcatt cggattaaac ctattgataa tatccaccac
      841 ctgaggtatt tctttccgct tgatgatggt gaggataatc tttacgtttc catatcggcc
      901 ttcggcatcc cagaaagtaa ggccataacc ccgttcactc aggtatgtga ttaggtcagt
      961 agtatcatat ctgggaataa ttctgataag tgctgtacca agcgagatac gttcttcgag
     1021 caatatgccg atatagttac ctgtggcaaa tcctgctcca taagccaagt agcatactat
     1081 attatcgagg tgtttaaaga tttgtgaaat ggccagcacc cagataatga cttcgaaaaa
     1141 tcctataaaa ggtgcaatgg ttttataccc tttcgaaatc aggatcaaac gaagggttcc
     1201 aatactttga tcgcaaattc tggcaaagaa aataaggaaa gggataatag cataagtata
     1261 aaatccagga tgagcttcaa accattctat catcgttaat caattttaaa ggtgaataat
     1321 tttgaattcg gttcggcggt tattggccct accttcgggt gtatcgttgg tatccactgg
     1381 tttagtttct ccatatccct tataggtcaa gcgcagtgga tcgatgtttt tactgattag
     1441 gtagctgtaa actgcccgag ctcgtttcag cgaaagttcc atattatagt cgtcgttgcc
     1501 ttggctatcg gtatgtccgg cgatttcgat ttgcaggtta gggttttgca tcaggagagc
     1561 ggccaggcgg tcgagttcgg ctcgtgattc gggcataagt tcgtaactat ccgtagcaaa
     1621 aaagacatta cgaagtacga ccgattcgcc aattttcata gcctgcatag gaacatcttt
     1681 caagaaagga tggtcataat cgtggttgcc ttcgaggtcg aagtggtcgg aataaaataa
     1741 atagt
//
LOCUS       DAORPD010000387         1742 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_12970_length_1742_cov_2.441020, whole genome shotgun sequence.
ACCESSION   DAORPD010000387 DAORPD010000000
VERSION     DAORPD010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12970_length_1742_cov_2.441020"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..302)
                     /locus_tag="PK976_05195"
                     /pseudo
     CDS             complement(1..302)
                     /locus_tag="PK976_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710583.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
     gene            789..1160
                     /locus_tag="PK976_05200"
     CDS             789..1160
                     /locus_tag="PK976_05200"
                     /inference="COORDINATES: protein motif:HMM:NF012354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRC67316.1"
                     /translation="MKNDHLILRMNYKIWMETEDGTPILGEGKCKLLKEIQRTGSLKI
                     AIENLGLSYRKTWNNLNRIEKKIGFHLVEKQRGGKEGGHTALTPQGEKVVEAFDRYYQ
                     EVDPLVKDLVGKVVLKFPHAG"
     gene            1247..>1742
                     /locus_tag="PK976_05205"
     CDS             1247..>1742
                     /locus_tag="PK976_05205"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRC67317.1"
                     /translation="MMKQTKITQEKPLSFDRFKKMVLKAIGRLGNPLPSIMLVCMAIL
                     LGPAQVYSQNIVKGKVLDANSHAAIRGANVIEESSKVGTTTSTSGEFSLEISKDVQKP
                     VLIISYLGYKTQKFAVYVEGKKFIEVFLEPEVYMVPELIVLGQRLSSKQNENAARIYV
                     VPEEE"
BASE COUNT          573 a          318 c          329 g          522 t
ORIGIN      
        1 agaaatttag gaaatttcga aaaatcgagt ccgcattttt caagaaaagc ttttttatct
       61 tcctgtgatt cttcgatgca ataataaagc aaggtagcct tatcggtcag tttccgtatg
      121 ccagcctgtc catcaagttc gacattcaac gacattttga tcatccttag tgcctgttga
      181 ctgtgtttca tcatggtttt aaaccaattc actactttat ctttaagctt attgaaaggt
      241 actaccttat ttataagccc aaattccaaa gctttttgaa cagtatattg acggcaaaga
      301 tagtcactta acctcaggaa cgacggggaa tggtaactct ttcttccttg ttcttgaaaa
      361 tttagttttt tcttttgccc cttaaactaa ccatcccaaa agtttgcatt tgcaataaaa
      421 atctaagtac aaataattca ttgaaactgt aatatccgaa aaatcaacca tcgagagtca
      481 ttgtgggttc ctctgcgcgg cagtgtagtt cgtttatccg cttcattttc atattatcgt
      541 ggtttggata agcatacaga ttcgaaatca tttttctctc ccttatcaat ttattggcaa
      601 acatttcagt aattcgagca tatagttcgg ttcagacagt caaataaaag gtcttctgag
      661 cacttgtaca agataaaagg cgataattat tttcgacgaa attttattta aataccaaaa
      721 tttcgattca tatcttggat ttcagtatat atcataaatt tgctcaactg ataaaagtat
      781 caaccatcat gaagaacgat catcttatct taagaatgaa ttacaagatc tggatggaaa
      841 ctgaggatgg tacaccgatt ttgggcgaag gcaagtgtaa gttactgaaa gaaattcaaa
      901 ggacaggttc tttgaagatt gccatcgaaa atttaggctt atcctatcgt aaaacatgga
      961 acaacctaaa ccggatcgag aaaaaaatcg gctttcatct agttgaaaaa caaaggggtg
     1021 ggaaagaagg cgggcacacg gctcttaccc ctcaaggcga aaaagttgta gaagcattcg
     1081 acaggtatta ccaggaagtt gatccactgg ttaaagattt agtggggaag gtagtattaa
     1141 aatttcccca tgcaggataa cgtggataaa tttcaattat atccgattcg tatatttgct
     1201 gacgaattgc aatatttttc aactccaaaa tctactaaaa cttaaaatga tgaaacaaac
     1261 aaaaatcacc caagaaaagc cattgagctt tgacagattc aaaaaaatgg ttttgaaagc
     1321 aataggacgg cttggtaatc ctttaccatc catcatgtta gtttgcatgg ctattttatt
     1381 agggccagcc caagtttatt cacaaaacat cgtcaagggc aaagtattgg atgcgaactc
     1441 gcatgcagcc attcggggtg ctaatgtcat tgaagaaagc agcaaagttg gaacaaccac
     1501 aagtacctca ggcgaatttt cgctcgagat ttcgaaagat gttcaaaaac cagttctcat
     1561 tatcagctat ttgggatata aaacccagaa gtttgctgta tatgtcgagg ggaaaaaatt
     1621 tatcgaagta tttctcgaac cggaagttta tatggtgcct gaattaatcg ttttagggca
     1681 gcgcctttcg agcaagcaaa acgaaaacgc tgcgaggatc tatgttgttc ccgaagaaga
     1741 aa
//
LOCUS       DAORPD010000388         1742 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_12971_length_1742_cov_2.303497, whole genome shotgun sequence.
ACCESSION   DAORPD010000388 DAORPD010000000
VERSION     DAORPD010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12971_length_1742_cov_2.303497"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            188..1237
                     /locus_tag="PK976_05210"
     CDS             188..1237
                     /locus_tag="PK976_05210"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HRC67318.1"
                     /translation="MKISENFFGRIEEKEIKIFELENPKGSKVSITNFGAIVQSLYVR
                     DREGNFEDIVLGFDSLEDYIQQTSYFGAIAGRYANRIAFAQFTLDGKTYQLSANDGNH
                     HLHGGFKGFDKVIWDARPYLDTTGACLELHYISKDGEEGYPGTLDVYVTYCFTLDDEL
                     KIYYQATTDHPTILNLTHHSYFNLSGFTQRNILEHELIIHADSFTPVDSTLIPTGEIV
                     PVKNTVFDFTSPRPIGSRIREVFNKKTGYDNNYVLNTTDSTVHPAAKAYDPESGRILE
                     VFTDQPGLQFYSGNFLDGKIKGKNGKIYQQHDGFCLETQHFPDSPNHPEFPSTVLRPG
                     QIFRSLTIYKFSTDR"
BASE COUNT          573 a          362 c          278 g          529 t
ORIGIN      
        1 gggcgcaaga tataaaaata ttttttccgt gaagcaaatc ctctcttttc tgattgtaaa
       61 aaatacaata attcaatagt tcaccatgaa aacaagctct aaaaaatttg tttatctaac
      121 tgtatttgtt attattacaa gtcagcttac tctttcctca tgccttaata tgaaatctga
      181 cactactatg aaaatctccg aaaatttttt tggtcgaata gaagaaaaag aaattaaaat
      241 ctttgaactc gaaaatccaa agggaagcaa ggtaagtata acgaattttg gtgctattgt
      301 ccaatcgttg tatgtaagag acagggaagg aaatttcgaa gatatcgtcc ttggattcga
      361 cagtttggaa gattatatac agcaaacctc atattttgga gccattgccg gccgctatgc
      421 caatcgtatt gcttttgcac aatttaccct cgatgggaaa acctatcaat taagcgccaa
      481 tgatgggaat catcatcttc atggaggttt taaaggattc gataaggtca tctgggatgc
      541 aagaccatat ttagatacca caggcgcatg tctcgaattg cattacatca gtaaggatgg
      601 tgaagaaggt tatcctggca cactggacgt atacgtaact tattgtttta cattagatga
      661 tgagctcaaa atttattacc aggccaccac cgatcatcct accatactta atcttaccca
      721 tcatagttat ttcaatctca gcggttttac ccagcgcaat atcctcgagc atgaactgat
      781 aattcatgcc gattctttca cacccgtcga tagtactttg atacccaccg gagaaatcgt
      841 tccggtaaag aataccgtct tcgattttac atcaccacgt ccgattggca gccgaatcag
      901 agaagtattc aacaaaaaaa caggctatga caacaactat gttttaaata cgacggattc
      961 cactgttcat cccgccgcta aagcttatga tcctgaaagc ggccgtattc tcgaagtttt
     1021 taccgaccaa ccaggattgc aattttattc gggaaatttc ctcgacggaa aaattaaagg
     1081 gaaaaatgga aaaatctatc aacaacatga tggcttttgc ttagaaacac aacatttccc
     1141 tgacagtcca aaccatcctg agtttccttc caccgtgctt cgacctggcc agatattccg
     1201 atcgctaacc atctataaat tctcgaccga tcgttaataa ggccttcaag catccgtaca
     1261 ccctgaataa cctaccatct tttaatctaa ccccaaaccc ccctccattc ccccattatc
     1321 cgtcttccat tttcccgcat ttggcgatgt ttttcacttt tcttagaatt acctttaatc
     1381 tctatgacgt ttaattaatg tttattctaa atgtttgagg aattcaacct atcctccatt
     1441 gagaaaatga gatggttaga gccaaagaaa acagtaaaaa tgaaaaattt cggtttttca
     1501 agcaacaaaa caacttaaca aatataagcc atgaatggag aaaatcaaaa tccattgcaa
     1561 acgaggaaga taggatttat catataaatt aatctctgac tgtaaaaatt caatcgaact
     1621 tgtatataat tttgttaagt gctttgatgt ttaaaaatta aaatctgttt gtatcataaa
     1681 attttaataa agccatttca tgaaaacttc agcgagattc ttactacctc ttcttataat
     1741 tt
//
LOCUS       DAORPD010000389         1732 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_13102_length_1732_cov_2.480024, whole genome shotgun sequence.
ACCESSION   DAORPD010000389 DAORPD010000000
VERSION     DAORPD010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13102_length_1732_cov_2.480024"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            260..1060
                     /locus_tag="PK976_05215"
     CDS             260..1060
                     /locus_tag="PK976_05215"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRC67319.1"
                     /translation="MYFASNIKLLRKRKGRTQDAVALALNMKRPTLSGYENGVAEPPL
                     GVLAQFSEYYKLSIDTLLKVDLAKLPESQLSQIERGYDPFVRGTQLRVLATTVDAQNE
                     ENIELVNEKAKAGYATGFADPDYVSILPTFRLPFLSRDKKYRTFQISGDSMLPIPDGA
                     YVTGEFVQNWLSIRDRHPYIILTLDDGIVFKVVENLIAQEGKLRLYSLNPVYQPYDVD
                     IRDVREVWKFINYITNQFPEPLPSTDLIYQTLNEVKDDVKAIRKKIDE"
     gene            1124..>1732
                     /locus_tag="PK976_05220"
     CDS             1124..>1732
                     /locus_tag="PK976_05220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRC67320.1"
                     /translation="MAKYSIIDVETTGTSIKGERITEIAIFNHDGQKITDEFVTLVNP
                     EKRIPYRISQITGITDRMLENAPRFCEIARKVLEMTEDSVFVAHNVGFDYGFVREEFK
                     NLGYRFERNKLCTVRLSRKLIPHHSSYSLGNICRDLGIQIFDRHRAGGDALATVKLFE
                     YLLGIDPNLSKINLKGINSLISQATLRALPEEAGVYYFYNASH"
BASE COUNT          494 a          406 c          353 g          479 t
ORIGIN      
        1 catgaggcta ccacgcctct gtcgcccatc ccaccaatga ttaccggttt gtcttcgagc
       61 ttgtggttgt aaagccgctc ggccgaaaca aaaaacgtgt ccatgtcgag atgcaaaata
      121 gtacgtcccg gctcatttat atatttcgtt gacaaaggca tgcatttcat agtaaatgat
      181 tattttttaa tcaaaatttc gatggcaata taatcatttt ttacaaaaaa tgttatcttt
      241 gcaaaaaaaa ttttgtctta tgtattttgc atccaacatc aaactgcttc gcaagcgtaa
      301 ggggcgcaca caggacgcgg tagccctggc tttgaatatg aaacgaccta ctctgagcgg
      361 atatgagaac ggggtggctg aaccacctct tggcgtactg gcgcaattct ccgaatatta
      421 caaactttct atcgatactc ttttgaaggt tgacttagca aagctgcccg aaagccaact
      481 ctcccagata gaacgtggat atgatccttt tgtgcgtggc acccaattaa gggtgctggc
      541 tactaccgtt gatgcacaaa acgaagaaaa catcgaactg gtcaacgaaa aggccaaagc
      601 cggttatgct acgggttttg ccgaccctga ttatgtaagc atactgccaa cttttcgcct
      661 gcctttcctt tcacgtgaca agaaataccg tacctttcaa atttctggag attccatgct
      721 tcctatccca gacggcgctt acgttacggg cgaattcgtc caaaactggc tgagcatccg
      781 cgaccgccat ccttatatca tccttacact cgacgacggc atagtattta aagtggtgga
      841 aaacctgatt gcacaagaag gtaaactccg actctattct cttaacccag tctatcagcc
      901 ctacgatgtg gacatacgcg acgtacgcga agtatggaaa ttcatcaatt acattactaa
      961 ccagtttcct gaacctcttc cttccaccga tcttatttat caaaccttga acgaagtgaa
     1021 agacgacgta aaggcaatcc gtaaaaaaat agatgaatga gattctcgca gtgtcattcc
     1081 caatcaatct taaatcaata agcctattat ccctactcag caaatggcta aatactctat
     1141 tattgacgtc gaaactacgg gcacttcgat taagggagaa cgcatcaccg agattgctat
     1201 attcaatcac gacgggcaga agattaccga tgaattcgtt accctggtga atcctgagaa
     1261 aaggattcct tatcgcataa gccagataac cggcatcacc gatcgaatgc tagagaatgc
     1321 acctcgattc tgtgagattg cccgcaaggt gctcgagatg accgaagact ccgtttttgt
     1381 tgcgcataat gtgggattcg attatggttt tgtgagagag gaattcaaaa atttaggcta
     1441 tcgcttcgag cgcaacaaac tatgtacggt tcgtctgagc cgaaaactca ttccccatca
     1501 ctcatcttac agtcttggga atatttgtcg ggatttgggt attcaaatct tcgaccggca
     1561 cagggctggt ggagatgctt tggccacggt caaactcttc gaatatctat tgggcattga
     1621 ccctaatctc tcaaaaatta acctcaaggg cataaattct ttaatttctc aggctacttt
     1681 gcgtgctttg cccgaagaag ccggagtgta ttatttttat aatgcatctc at
//
LOCUS       DAORPD010000390         1731 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_13118_length_1731_cov_2.449881, whole genome shotgun sequence.
ACCESSION   DAORPD010000390 DAORPD010000000
VERSION     DAORPD010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13118_length_1731_cov_2.449881"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            269..682
                     /locus_tag="PK976_05225"
     CDS             269..682
                     /locus_tag="PK976_05225"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRC67321.1"
                     /translation="MEEFIEIISQLSHLIGEAEEKAREDYHKLHLTITQMHYLEMINF
                     MHNPNITELAAQLNLSKPSVKAVVDKLIEKSCIYKVRSDADRRSTHLHLTEKGKLIQQ
                     MHDKAHRNIAELFEHKLTEEEIGKLEELLTKVLRE"
     gene            856..>1731
                     /locus_tag="PK976_05230"
     CDS             856..>1731
                     /locus_tag="PK976_05230"
                     /inference="COORDINATES: protein motif:HMM:NF012350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HRC67322.1"
                     /translation="MITNLQEYTVRGLTHEEVERRLAMEGYNELPSSRPRNLFQIALG
                     VLKEPMFILLVVCGSLYLILGDLQEGLMLLSFVFVIMGIEFYQEKKTERALDALRNMA
                     SPRALVIREGETIRIPGREVVKDDIVVLQEGDRVPADAIVLSCVNLSADESMLTGESI
                     PVRKREWSEDDVTFSPGGDDVPLVYSGSMIVGGNAIVKVLATGIHTEIGKIGKALESV
                     KEEPTQLKREMGMLVKRLTWMGAILCVMVIVIYTLTRGNLLKGFLAGITLAMAMLPEE
                     FPVVLTVFLALGAWRI"
BASE COUNT          539 a          278 c          431 g          483 t
ORIGIN      
        1 gccataacca acctctcaag gatttcatta cggatctgac tgaagggtgt catagaatga
       61 atataccgga attgattgat gccatttcga ttcatttgaa ggtttccagc ttgttcatcg
      121 agcattgtga gtaatatgcc tgaaataaac ccagaccaga aaagcagtca ttatgttttt
      181 tgattagcta aatgactgat tcgataaaaa atttcaattg aaacttattt ttaaaaatta
      241 attagtaaaa actaactatt tttgttgcat ggaagagttc atcgaaatca tatcgcaatt
      301 atcacatttg attggagaag ccgaggaaaa ggcacgcgaa gattatcaca aacttcatct
      361 caccataacg cagatgcatt atctggagat gatcaacttt atgcataatc ccaatattac
      421 tgaacttgct gcccagttga acctatcgaa acccagtgtg aaagctgtag tggataagct
      481 catcgaaaag agttgcattt ataaggtaag gtctgatgcc gacaggcgta gcacacattt
      541 gcatctcacc gaaaaaggaa aattgattca acaaatgcac gacaaggctc acagaaacat
      601 agctgaatta tttgagcata aactcacaga ggaggaaatt gggaaactgg aagagctttt
      661 gacgaaggtt ttgagagaat gaaacgaaat ccttgataag gctgagacta caagagtata
      721 caaatcatct ggaaggaaaa ttgttaattg ggatttttca ttttgattgc tttttagaat
      781 gaataaatga gatataagat ggtttcattg gttttgaaaa aatagttagc aaatcctaac
      841 aagataaaaa agaaaatgat aacgaatctt caagaatata cagtaagggg tttaactcat
      901 gaagaggtag agcggaggct tgccatggaa gggtacaatg agctgccatc atcaaggccg
      961 aggaatttat ttcagattgc gctgggagta ctcaaagagc ctatgtttat tctactggtc
     1021 gtgtgtggta gcttgtatct gatattgggg gatcttcagg aaggcctgat gctgctgagt
     1081 tttgtctttg tcatcatggg gatagaattt tatcaggaaa agaagacgga gagagcactc
     1141 gatgcattga gaaatatggc aagtccacga gcattggtaa tacgtgaagg tgaaaccata
     1201 aggattcccg ggcgcgaagt agtgaaggat gatattgtag ttttacagga aggcgatcgt
     1261 gttccggccg atgctatcgt tttgagctgt gtgaatctat cggccgatga gtcgatgcta
     1321 acgggtgaat ctattcccgt gaggaagaga gaatggagcg aagatgatgt gacctttagc
     1381 cctggaggcg atgatgttcc cctggtttat tctggttcga tgatagttgg agggaatgcc
     1441 atcgttaaag ttttggctac tggaatacac acagagatcg gtaaaattgg caaggcgctg
     1501 gagtcggtga aagaagagcc cacacaattg aaaagggaaa tgggaatgct ggtaaaacgg
     1561 cttacctgga tgggggctat tctgtgtgta atggtgatag tcatttatac cctcacaagg
     1621 ggcaatttgc tgaaaggttt tttggcgggt attacacttg ccatggctat gctgcctgag
     1681 gaatttcccg ttgtattgac ggtttttctg gcgttgggag cttggcgaat c
//
LOCUS       DAORPD010000391         1728 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_13145_length_1728_cov_2.536163, whole genome shotgun sequence.
ACCESSION   DAORPD010000391 DAORPD010000000
VERSION     DAORPD010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13145_length_1728_cov_2.536163"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /gene="rodA"
                     /locus_tag="PK976_05235"
     CDS             <1..675
                     /gene="rodA"
                     /locus_tag="PK976_05235"
                     /inference="COORDINATES: protein motif:HMM:NF037961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HRC67323.1"
                     /translation="LFVLALVLDKYILIGLCALILLVYYLTRRNRKKLHWILIGIFIA
                     TSSITFSVDYVFHNVLQPHQRSRINVLLGKETDIKGAGYNVYQSMIAIGSGGFWGKGY
                     LKGTQTKYNYVPKQSTDFIFCTIGEEWGFVGSLILIGLYVGLMLRILQLAERQRSAFS
                     RIYGYGVASIIFAHFAINIGMTIGLLPVIGIPLPFVSYGGSSLLAFTILLFIFIKLDA
                     HRHDII"
     gene            924..1619
                     /locus_tag="PK976_05240"
     CDS             924..1619
                     /locus_tag="PK976_05240"
                     /inference="COORDINATES: protein motif:HMM:NF040157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5714 domain-containing protein"
                     /protein_id="HRC67324.1"
                     /translation="MPEESCRICGNQILETKEFHSVRGYYCKQDNQLKKTCKQFHYIC
                     FGSTNQLINEMIFTLCMDTGLSDPDEIDLQAFDHLGIPVHGPQHHFLISMALTVAYLN
                     SIHHNYKKEEYLKEKRERSERIPGGFCWTYDTCGAAIIVGMFISVIIRAQWFSEKEWQ
                     LSNMAITRTLKSIAKVRRPRCCKRVTFISSRNAQNFLEKLLQGKLSVHVYIVCHPHDL
                     NKECFQNRCMFHF"
BASE COUNT          517 a          349 c          336 g          526 t
ORIGIN      
        1 ctgttcgtac tggcactcgt tctcgataaa tacatcctca taggcctatg tgctctcatt
       61 ctgctggttt attatctgac tcgaagaaac aggaaaaagc ttcactggat cttgatagga
      121 atatttatag ctacttcttc cataaccttt agcgtggatt atgtattcca taatgtttta
      181 cagcctcacc agcgaagccg gataaatgtg ttgttaggaa aggaaaccga tataaaaggc
      241 gctggatata atgtctatca aagcatgatt gccattggta gtggaggttt ctggggaaaa
      301 gggtatttaa agggcacaca aaccaaatac aattacgttc ccaaacaaag caccgacttt
      361 atattttgta ccatcggaga agaatggggc tttgtgggaa gtctgattct cattgggctt
      421 tatgtggggt taatgctcag gatattacaa ttagccgaac ggcaacgatc ggcgttttcg
      481 agaatatacg gatatggtgt ggcttcaatc attttcgctc atttcgctat taacattgga
      541 atgactattg gcctactccc agtaatcggg attcccttgc ccttcgttag ctatgggggt
      601 tcttctttac tggcgtttac cattttattg tttattttta tcaagctcga tgctcataga
      661 cacgatatca tctgaggaaa ccaaacaacc cgcattggtt atgattcatt ccccttctga
      721 aatcaggaag agcaaattat tataaactat tctccagtcg ttccatttcg atcttcgatt
      781 catagatcgt cgaaagcaac cggcatgaag gattcgcgtc atattcgaat aaacataagg
      841 gcagcctcaa cattcttctc ctaccaataa tatttcgcta tttcagagaa aaaatatagg
      901 tttgccataa atctctccat tcgatgccag aagaaagctg tcggatttgt ggcaatcaaa
      961 tattagaaac taaagaattc cattcggtaa gaggctatta ttgtaagcag gataaccagc
     1021 taaaaaaaac ctgtaaacaa tttcattaca tctgctttgg ctccaccaat cagctcataa
     1081 atgaaatgat attcactctc tgcatggata caggcttaag cgatccggac gagattgacc
     1141 tgcaggcatt cgatcatctt ggaattcccg ttcacgggcc acaacatcat tttttgattt
     1201 ctatggctct cactgttgca taccttaatt ctatccatca taactataaa aaagaagagt
     1261 atctaaagga aaaaagagaa cgctcagaga ggattcccgg aggtttttgc tggacttatg
     1321 atacctgtgg ggcagccata atcgtgggta tgtttattag cgtgattata cgagcacaat
     1381 ggttttcgga aaaggaatgg caactttcga acatggccat aacccgaacg ctcaaaagca
     1441 ttgcaaaggt acgaaggcct cgctgctgca aaagggttac gtttatctca tcgagaaatg
     1501 cacaaaattt cctcgaaaaa ctgttacagg ggaaactctc cgttcatgtg tatatagtat
     1561 gccatccaca tgatctgaac aaagaatgtt ttcaaaatcg ctgcatgttt catttttgaa
     1621 ttatgaaaat taagtcatcc tacaatgatc attgtttgtc aataaaatta tatgcttcct
     1681 ccaagatcca tcttcttatt ttgacctttt cgatagaatc gtactttt
//
LOCUS       DAORPD010000392         1728 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_13149_length_1728_cov_2.201435, whole genome shotgun sequence.
ACCESSION   DAORPD010000392 DAORPD010000000
VERSION     DAORPD010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13149_length_1728_cov_2.201435"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..691)
                     /locus_tag="PK976_05245"
     CDS             complement(92..691)
                     /locus_tag="PK976_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007483040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP diphosphatase"
                     /protein_id="HRC67325.1"
                     /translation="MKNIELVFATNNPHKLREASQILGPNIEILMLNEIGCFDEIPED
                     GATVEANASIKSWYVYRHYQKNCFADDTGLEIDTLGGRPGVFSARYAGENKSFDDNIR
                     KVIYEMKGKTNRRARFRTVISLIIQGKEYFFEGIVEGKIIENKRGNMGFGYDPIFIPN
                     GYSRTFAQMDEAEKNHISHRGIALRKMAEFLEYKKIFEQ"
     gene            complement(743..1504)
                     /locus_tag="PK976_05250"
     CDS             complement(743..1504)
                     /locus_tag="PK976_05250"
                     /inference="COORDINATES: protein motif:HMM:NF013395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HRC67326.1"
                     /translation="MSKNFIWSIVFFILMIREGGLFAQPEIWCLPEYSEDELVIRHVG
                     FTLKYDKNHKLSDWVAYDIVPYKLLKSVKKSNHFIPDPQLPDEWSAHSIDYKYSGYDR
                     GHLAPAADMAWSAQAMKESFYLSNICPQQPQFNRITWKKLEEKVRQWARIYDTLLITT
                     GPVLSSDLPKIGHGKVSVPKYFYKAVVIYTHHKKSGIAFVMQNSYSSKVLMQSAITID
                     SLERLTGFNFFARLPEEEQLNIEGKLIIEDWNILR"
BASE COUNT          489 a          354 c          308 g          577 t
ORIGIN      
        1 cagtatagtt cctaaaatgg ccatcaaggc tggtttcgag ctcggcaaat tttatgattt
       61 gttaatcgag gaagctctgc gagattgaat tttattgttc gaatatcttt ttatattcaa
      121 gaaattcggc catttttctc aaagcaattc ctctgtggct tatgtgattt ttctcagctt
      181 cgtccatctg ggcaaaagtt cggctataac catttggaat aaaaatagga tcgtacccaa
      241 aacccatatt acccctttta ttttcgatga ttttgccctc tacgattcct tcgaaaaaat
      301 attcttttcc ctggatgatg agagaaatca ccgtacgaaa tcgggcacgt ctgttagttt
      361 tccccttcat ttcgtagatt accttccgga tgttgtcgtc gaatgattta ttttccccag
      421 cataacgcgc cgagaaaacc cccggacgtc ctcctaaagt atctatctcc aatcctgtat
      481 cgtcggcaaa acaattcttc tgataatggc ggtacacata ccacgatttt attgaagcat
      541 tggcctcgac cgtagcacca tcttcgggaa tttcatcgaa acatccgatt tcatttagca
      601 tcagaatctc aatatttggt cctaaaattt gtgaagcttc ccttagttta tgtggattgt
      661 ttgtagcaaa aacgagttca atatttttca tttccagatt gttattcgtc ttttatcaat
      721 catatatcat atatcttgta ttttatctta atatattcca atcttctatg atgagcttcc
      781 cttcgatatt aagttgttcc tcttctggaa gacgagcaaa gaaattgaat cctgttagcc
      841 tttccagcga atcaatagta atggccgatt gcatcaaaac cttggaagaa taactatttt
      901 gcattacgaa agctattccc gatttcttgt ggtgtgtgta aataactact gccttataaa
      961 aatactttgg cacactcact ttaccatgtc ctatttttgg aaggtccgag ctaaggacag
     1021 gacctgttgt aatgagcaag gtatcataaa tccgagccca ctgccgtacc ttttcttcaa
     1081 gctttttcca cgttatgcgg ttaaattgag gctgctgagg acaaatatta ctcaaataga
     1141 atgattcttt cattgcctgc gccgaccatg ccatatcagc ggctggtgct aaatgacccc
     1201 ggtcgtaacc actatattta taatctattg aatgagccga ccattcatca ggcagttgtg
     1261 gatcgggtat aaaatggttg gattttttca ctgattttag aagcttatag ggcactatgt
     1321 cgtaagctac ccaatcggaa agtttgtgat tcttgtcata tttcaaagtg aatcctacat
     1381 gcctgatgac aagttcatcc tcggaatact ccggtaaaca ccatatttcc ggttgagcaa
     1441 ataaaccccc ttccctaatc attaaaataa aaaagactat tgaccagatg aagttcttcg
     1501 acatcaatac gaattatgac ttcaaaatta caaagtttta gcattttgat attgatgagg
     1561 ccttggatac ttttgttgaa gcttattcta attatgctat cctgataaaa acggcaaaag
     1621 cggtttttaa ctgaatgaat gattagccga ggaggaaatg ttcattcttc atattgtata
     1681 gtcattttac aagttacaag cacttattta ggccaaatgg cttcgggg
//
LOCUS       DAORPD010000393         1727 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_13156_length_1727_cov_2.851675, whole genome shotgun sequence.
ACCESSION   DAORPD010000393 DAORPD010000000
VERSION     DAORPD010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13156_length_1727_cov_2.851675"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="PK976_05255"
     CDS             <1..825
                     /locus_tag="PK976_05255"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRC67327.1"
                     /translation="GFSVEFAEHRLYNTGEPIRNIDWKLYARTDKLFVKRFEEETNLR
                     CQLVMDTSASMYYPPIQYPDVEHPTKLLFSIYAAAALMFLFKQQRDAVGLSIFNNDIS
                     QTIQARSSAIHIHYLFTELEKLIESQPPSRPHESSIADSLHLIAESIHKRSLVIIFSD
                     MFDKAGEPELLFHALQHLRHNKHEVILFYTIDKQLELNFDFENRPYRFVDIENGKEIK
                     LNPYELRERYMTSMQNFLDELKFRCGQYHIDLVEADIHEGFNQILLPYLIKRSRLY"
     gene            complement(1005..1628)
                     /locus_tag="PK976_05260"
     CDS             complement(1005..1628)
                     /locus_tag="PK976_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009123123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HRC67328.1"
                     /translation="MDEKYYICIGRQIGSGGKKISEKLAQILQLPVYDKELIQMASRK
                     SGLRSEIFEQFDEKGSRSLIGGLLGMSSPIPDEIYAQYYLTNESLFVIESDVIRELAA
                     QKSCIFLGRCADYVLKDCERCLNVFICANYEDRIKTIAETYHYQVNKAKDFIKKMDKK
                     RADYYNYFTNKHWGVADSYHLCINSSTLGIDETVNFIYLFVKKNFGI"
BASE COUNT          523 a          368 c          292 g          544 t
ORIGIN      
        1 ggcttttcgg ttgaatttgc cgaacatcgt ctatacaata ccggcgaacc tatccgaaat
       61 atcgattgga aactttatgc ccggactgat aaattattcg tgaagcgatt cgaagaagaa
      121 acgaatcttc gctgtcaact ggtcatggat acctcggcct cgatgtatta tccacctata
      181 cagtatcccg atgttgaaca tcccactaaa cttctttttt cgatttatgc tgccgcagct
      241 ctgatgttct tgtttaaaca gcaaagagat gcagtgggct tgtcgatctt taataatgac
      301 ataagccaaa ccattcaggc tcgctccagt gccattcata tccattattt attcaccgaa
      361 ttagagaaat taatagaatc gcagcctcca tctcgtcccc acgaaagttc cattgccgat
      421 tcacttcatt tgatcgccga atccattcac aaaagatcac tggtgatcat tttcagtgat
      481 atgttcgata aagccggtga gccagaatta ttattccatg ctttgcaaca tcttcgacat
      541 aacaaacatg aagtcattct tttctatact atcgacaaac agctggagtt aaacttcgac
      601 ttcgagaacc ggccttaccg ttttgtggat atagagaacg gaaaagagat caaactaaat
      661 ccctacgaac tgagagaacg atacatgact tcgatgcaga attttctgga tgaattaaaa
      721 tttcgttgcg gtcaatatca tattgatttg gtcgaagccg atattcacga aggattcaac
      781 cagattctac tcccttacct aattaagcgc tcgagacttt attaggttcg atggacaaag
      841 gataatgatt gaggatgaaa gttgtaagtt gtttgaattt tactacgagt taggtggagt
      901 gaatttcacg gtagctggta tttattgctg tatcactcag gcatatggct atattcccca
      961 taagtcaccc ttccccatcc accacgttca tcacctttca catatcagat tccaaaattc
     1021 tttttaacaa acaaataaat gaaatttact gtttcatcga taccaagggt ggatgaattt
     1081 atgcagagat ggtatgaatc ggcaacgccc caatgtttat tcgtaaaata attgtaataa
     1141 tctgctcttt ttttatccat tttcttaata aaatctttgg ctttattcac ctgataatga
     1201 taagtctccg caatggtttt tattctatct tcataattgg cgcaaataaa tacattcaaa
     1261 caacgttcgc agtctttcaa cacataatct gcgcaacggc caaggaagat acatgatttt
     1321 tgtgcagcta attcacgaat cacatcactt tcgatcacaa ataatgattc attggttaaa
     1381 taatattgtg catagatttc atcgggtata ggactgctca ttcccagcaa tccgcctatc
     1441 agactacgac ttcctttttc atcaaattgc tcgaaaatct cgcttcgtaa accgcttttt
     1501 ctggaagcca tttggataag ttccttatca tagacgggca gttgtaagat ttgggcaagt
     1561 ttttcgctaa ttttcttccc cccgcttcca atctgacgtc ctatacagat gtaatatttt
     1621 tcatccataa ttcgaagatt tgaactaaca aacttacata aaaaaataat ttatatacgc
     1681 cgcacaacaa aaatcaaggc catgattgtt ctataagtaa tcaacgt
//
LOCUS       DAORPD010000394         1727 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_13160_length_1727_cov_2.109450, whole genome shotgun sequence.
ACCESSION   DAORPD010000394 DAORPD010000000
VERSION     DAORPD010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13160_length_1727_cov_2.109450"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1029)
                     /locus_tag="PK976_05265"
     CDS             complement(<1..1029)
                     /locus_tag="PK976_05265"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HRC67329.1"
                     /translation="MRNKKKKILWALSLIFSIIVFPEITLGDDPIAIDRMITITRELP
                     PFHGVEAGGTFNVVINIGQLQEVKIEVDEDQIENVITEVVNEILYIKGNKLKNPLVNI
                     SITIPELKYLNASGAATFKLNGMNRVKEMKLMASGAANVELNVEIDHLLSDVSGAADV
                     KVNGRASWHKSDVSGAGSLKASELVSDTLIIFLRGAADAFVDVKDLAIVQKSGAANIK
                     YRTKPRILTDVGYSTQYKIDTADKGVVNLGGDSVVVDLGKLKIKVDDNEGVSTIKIGK
                     QKLIVDDDGNVKWKKETNQHCFTGHWGGFDLSLNGYLNSDGNMDFAGADRYLDLNMPK
                     SIGVHLNAY"
     gene            1176..>1727
                     /locus_tag="PK976_05270"
     CDS             1176..>1727
                     /locus_tag="PK976_05270"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK/PrmC family methyltransferase"
                     /protein_id="HRC67330.1"
                     /translation="MTLEISNNRVYHIYRHYRHKLSSIYPSEEASTIARMVMQEILGI
                     DQIKFLSEPDLRISESEILKIHFAFKDLLNHRPLQYVLGYAWFRNIRLKVGHGVLIPR
                     PETEELVGWILENFLDKPNLRILDIGTGSGCIAISLAKELKGAQITATDISEMALEYA
                     RENASEHGTAIQFVLHDITSNAKS"
BASE COUNT          494 a          395 c          276 g          562 t
ORIGIN      
        1 ataggcattt aagtgaactc caatggattt aggcatattc aaatcaagat aacgatcagc
       61 accggcaaaa tccatatttc catcactgtt taagtaaccg ttcaaactta gatcgaatcc
      121 tccccaatgc cctgtaaaac aatgttggtt cgtttctttc ttccatttca cattgccgtc
      181 atcatcgacg attaatttct gtttcccaat tttaatcgta cttacgcctt cattatcatc
      241 cactttaatt ttcaatttac ccagatcgac aactaccgag tctcctccca gattgactac
      301 acccttatct gcagtgtcga ttttgtactg ggtagaataa ccgacatctg tcaaaatacg
      361 aggtttcgtc ctatatttga tatttgcagc cccgcttttc tgcacaattg ccaaatcttt
      421 tacatcgaca aaggcatcgg ctgctccacg taaaaatatg atcaaagtat cgcttaccag
      481 ttccgaagct ttcaaacttc cagctccact tacatccgac ttgtgccaac tcgccctgcc
      541 attcacctta acatcggcag ctcccgatac atccgaaagc aaatgatcta tttcaacatt
      601 cagctcaaca ttggctgcac cactggccat aagtttcatt tcttttaccc gattcatccc
      661 atttaacttg aatgtagctg caccacttgc gtttagatat ttcaattctg gtatggtgat
      721 agatatattc acaagaggat tcttcagttt attcccttta atgtataaaa tttcattcac
      781 cacttccgta atcacatttt ctatttgatc ttcatcgact tcgattttca cttcctgtaa
      841 ctgaccaatg tttattacca cattgaaagt gcctcccgcc tcaactccat gaaaaggggg
      901 caattctctt gttatcgtga tcatcctatc aattgctatg ggatcatcac ccagcgtgat
      961 ttccggaaaa acaataatcg agaatatcag gctaagtgcc caaagaatct tcttcttttt
     1021 gtttctcatt gctataaatt ttgaatttct tggtttttga attttattta tgacgaattt
     1081 ttacttcaga tgttacaaat attcggtttg tttattcatt atacgaattc gaatatcttt
     1141 caattatagt tctggaaact tactaacttt gctgcatgac acttgaaata agcaacaatc
     1201 gggtttacca tatttaccgg cattatcgtc acaaactttc ttcgatttat ccttcggaag
     1261 aagcatccac catagctcgt atggtgatgc aggaaatttt aggaattgac caaataaaat
     1321 ttctttccga acccgatctg cgtatttccg aatcagaaat cctaaaaatt catttcgcct
     1381 tcaaggattt attgaatcat cgacccctac aatatgtact aggttatgca tggtttagga
     1441 atatacggct aaaagtcggc catggcgtgc ttattccaag gcctgaaacc gaagaactgg
     1501 ttggttggat actcgaaaat tttttggaca aacccaattt gagaattctt gatataggaa
     1561 cgggaagtgg ctgtatagca atttcattgg caaaagagtt gaaaggtgcc cagattacgg
     1621 caaccgacat ttcagaaatg gctctcgagt atgcccgcga gaatgcttca gaacatggaa
     1681 cagccattca gtttgtcctc catgatatta cttccaatgc aaaatca
//
LOCUS       DAORPD010000395         1724 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_13186_length_1724_cov_3.118634, whole genome shotgun sequence.
ACCESSION   DAORPD010000395 DAORPD010000000
VERSION     DAORPD010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13186_length_1724_cov_3.118634"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="PK976_05275"
     CDS             <1..793
                     /locus_tag="PK976_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HRC67331.1"
                     /translation="MELGIVDATLVGDEATIVKVCKDLNIQPEKFRIVQESDEQKAAH
                     KAVELIVKGEGDILMKGLVTTDKYMRAILNKDAGLLPPKAILSHVTVIENPNYEKLLI
                     VGDVAIIPVPDLDQKIAITNYLIQTAHRLGNEKPKVAIIAATEQMLPNMPTCVDAAII
                     SKMGERGQIKGALIEGPLALDTAIDKESAAIKKLKSEVAGDADCLLFPNIETGNVFYK
                     FNTKLAKGELGAIVIGAKCPAVLSSRGDSALTKTYSIALAALSTD"
     gene            821..>1724
                     /locus_tag="PK976_05280"
     CDS             821..>1724
                     /locus_tag="PK976_05280"
                     /inference="COORDINATES: protein motif:HMM:NF013665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HRC67332.1"
                     /translation="MITNFTQLVDKLNTAAPQTIAVVAAEDHATMGAIVRAIRTGFAK
                     AILIGNKTRTESLLAHLELPPETFLIVHLPDEQMAMKEALSLIHQGKADILMKGLIGT
                     DIFLKAVLDKTSGLMKQGEVMTYVAALQIPSYHKLLFISDTAVIPYPNLEQKIAMLKY
                     AVEMARRFGISKPKVALIGASEKVGNQSQTAIDASIICKMAQRGQLGEVIVDGPLDIF
                     LATQPESLNIKRIITPIGGEADILIFPSLESCNAFYKGLVRFAGAELAGLLQGTRKPV
                     VLMSRSESENSKFYCLALACLMANN"
BASE COUNT          519 a          391 c          384 g          430 t
ORIGIN      
        1 catggaatta ggaattgtag atgctacgct tgttggcgat gaagccacta ttgtaaaggt
       61 ttgcaaggat ttgaatatcc aacccgagaa atttcgcatt gtacaggaat cggatgagca
      121 aaaggcagct cacaaagccg ttgaattgat tgtaaaggga gaaggcgata ttctgatgaa
      181 aggccttgtg accaccgata aatatatgag ggctattctc aataaagacg caggtttgtt
      241 gccaccaaag gcaatcttga gtcatgtaac cgttatcgaa aacccgaatt acgaaaaact
      301 gttaattgtt ggtgacgtag ccattatacc tgttcccgac ctcgaccaga agattgccat
      361 cacgaattac ctgatacaaa ctgctcatcg tttaggtaat gaaaagccta aggtggcaat
      421 tattgccgca acagaacaaa tgttaccgaa catgccaaca tgcgtggatg cagccataat
      481 ttcaaagatg ggtgaacgcg gacaaatcaa aggagcgctt atagagggtc ctcttgcact
      541 cgacacggct atcgacaaag aaagcgctgc cattaaaaag ctgaagagcg aagtggccgg
      601 cgacgccgat tgtctgcttt tccctaatat cgaaacaggt aatgttttct acaaattcaa
      661 tacaaaactc gccaaaggcg aactgggagc catcgtcata ggagccaaat gcccggcagt
      721 cctctcctca agaggcgata gcgcccttac taaaacctat tccattgccc tggctgctct
      781 gtcaaccgat taatgaccca tatttcgtca tttcccggct atgatcacga attttacgca
      841 attggtggat aaattaaata cagcagcccc tcaaaccatt gctgtggtgg ccgccgaaga
      901 tcatgcaacc atgggagcca tcgttcgggc cataagaacg ggatttgcca aggctatcct
      961 tatcggcaac aagacccgaa cagaatcatt gcttgcccac ctggaacttc ctcccgaaac
     1021 ttttttgatc gttcatctac ccgatgaaca aatggcaatg aaagaggcat taagtctgat
     1081 tcatcaagga aaggccgata tcttgatgaa aggactcatt ggtacggata tttttctgaa
     1141 agccgtgctg gataaaacat cgggactgat gaaacagggc gaagtaatga cttatgtagc
     1201 tgctctgcaa atcccttctt accacaaact acttttcatt tccgatacgg cagtcatccc
     1261 ttatcccaat ctcgaacaga aaattgccat gttgaaatac gcggtggaga tggcaagaag
     1321 gtttggcatt tccaaaccca aagttgcttt gattggagcc tccgaaaaag tcggaaatca
     1381 atcacaaaca gccatagatg ccagtattat atgcaagatg gctcaacgcg gacaacttgg
     1441 ggaagtgatt gtggatggcc cactcgatat ttttcttgct actcagcccg aaagcctgaa
     1501 cattaaacga atcatcactc ctattggagg agaagccgat attttgatct ttccttctct
     1561 cgaaagttgc aatgcattct ataaaggcct tgtaaggttt gctggcgcag aattggctgg
     1621 cttgctccaa ggaacacgca agcccgtagt tctgatgtcg agaagcgaaa gcgaaaattc
     1681 gaaattttac tgtttagcac ttgcctgcct catggcaaat aata
//
LOCUS       DAORPD010000396         1724 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_13190_length_1724_cov_2.216896, whole genome shotgun sequence.
ACCESSION   DAORPD010000396 DAORPD010000000
VERSION     DAORPD010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13190_length_1724_cov_2.216896"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1571
                     /locus_tag="PK976_05285"
     CDS             <1..1571
                     /locus_tag="PK976_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67333.1"
                     /translation="LNIHIKTVGEKICFGFGDRIDNYGGNISDVQYRLRKPDGTIAMG
                     PANLPTSGAGYISTYNQATIGPSNLTGNTGGYTPLTHTATMTGDYYIEFSFNYSGWGS
                     PDRCRFKYFDITVGNSANQEVDGRVWSKAWQFTSGNQNNEFTGSLFVYADDGIVTKID
                     FNGIQPYVFTVYCNPTGITNTGVFEVDRKSKLGTSYTVNAQYKIFLNDPDITVYPTGA
                     FGSITGPITTDSHCDGNLDIFVPVNKTGKVDIFFNINPLPGTQAEDLSLTANVNVGNN
                     TITWNGLNGMGLPVPSGTVFTITVTYINGLTNLPMRDPDTHPYGFKVSLIRPAGPSPA
                     LFWDDSEVGGSVNLDGCISTTGCHSFSYSVGNNNYINTWWYASSTTGDPVTVNYRRSY
                     MGTINTSICEGDSILINGIWVYAGGTYVNNLMNQQGCDSIVTTNLTLRPTPIVNLGPD
                     TTVCNGLPVQFTAPTGIGYQYLWSTGATTQTIIVTQSGTYTVNVTNSAECTGQGSVNY
                     TSYPKPPPKPIYHD"
BASE COUNT          527 a          353 c          332 g          512 t
ORIGIN      
        1 ggctcaatat tcatattaaa actgttggag aaaaaatctg ttttggcttt ggcgatcgta
       61 tcgacaacta cggtggaaac atttcagatg tacaatatcg attacgtaaa cccgatggca
      121 ctattgctat ggggccagcc aaccttccca cttctggtgc aggttacatt tcgacctata
      181 atcaagcaac cattggcccc agtaatttga ctggaaacac aggcggttac actcctttga
      241 cacatacagc taccatgaca ggagattact atatcgaatt ttcatttaat tactctggtt
      301 ggggttctcc cgaccgctgt cgtttcaaat acttcgatat tacagtagga aattcagcta
      361 atcaagaagt tgatggaaga gtatggtcaa aagcatggca atttacctcg ggaaatcaaa
      421 acaatgaatt tacgggaagc ctttttgttt atgccgatga tggcatagta actaaaatag
      481 atttcaatgg tattcagcct tatgtattta ccgtttactg caaccccacg gggattacta
      541 atacaggagt tttcgaagta gatcgtaaat caaaacttgg cacttcttat acagtaaatg
      601 cgcaatacaa aatttttttg aatgatcccg atattactgt ttatccaact ggagcatttg
      661 gatccataac cggacccatt acaacagata gtcattgcga tggcaacctt gatattttcg
      721 ttccagtaaa taaaaccgga aaggttgata ttttttttaa tatcaatcca cttccaggta
      781 ctcaagccga agatttaagt ctcaccgcta atgtaaatgt cggcaataat actattactt
      841 ggaatggatt gaatggaatg ggtttgcccg tgccaagcgg tacggttttt acgattacgg
      901 taacttacat taatgggctt actaatcttc ctatgcgcga tccagataca catccctatg
      961 gttttaaagt ttcattgatt cgacctgcgg gaccctcccc cgcccttttc tgggatgatt
     1021 ctgaagtggg cggttcagtc aatctggatg gctgcatatc gactacaggt tgccattcat
     1081 tttcatattc agtggggaat aataattaca tcaatacttg gtggtatgca tcaagcacta
     1141 ccggagaccc ggttacggta aattatcgcc gctcttatat gggaaccata aataccagca
     1201 tttgtgaagg ggattcgatc ttaattaatg gaatttgggt atatgctggg ggaacgtatg
     1261 tgaataatct gatgaatcag caaggctgcg atagcattgt tacaacaaat ctaaccttga
     1321 ggccaactcc catcgtaaat ttaggccctg atacaactgt ctgtaatggt ctaccagttc
     1381 aatttactgc tcccacgggc atcggctatc aatacctatg gagtacaggt gcaacaactc
     1441 aaacgatcat tgttacacaa agtggaactt ataccgttaa cgtaaccaat agtgcagaat
     1501 gcaccggcca aggatcggta aattatacca gttatcctaa accccctcca aaacccatct
     1561 atcacgattg atatcttttt aactatcaat agattaaaca cgtattcgcc taaaggtttt
     1621 aatagggttt aaaagatata aaggtctttc taaatcatta tcatccgcat aatttagccc
     1681 aagaatataa aaaaacaagg tatggtataa catttttcat gtaa
//
LOCUS       DAORPD010000397         1724 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_13191_length_1724_cov_2.007789, whole genome shotgun sequence.
ACCESSION   DAORPD010000397 DAORPD010000000
VERSION     DAORPD010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13191_length_1724_cov_2.007789"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="PK976_05290"
     CDS             <1..272
                     /locus_tag="PK976_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67334.1"
                     /translation="VDQLMTDFKNIEILIISGLYAIELEEADLKVFIELTYRETLDVQ
                     RESEKEELDDFRMKVLEQEHKAVQALKHKADFFVDFDTALEIFHL"
     gene            complement(496..>1724)
                     /locus_tag="PK976_05295"
     CDS             complement(496..>1724)
                     /locus_tag="PK976_05295"
                     /inference="COORDINATES: protein motif:HMM:NF025620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HRC67335.1"
                     /translation="TINTSGAAILEASGSGYDPSYCTAIKCNHSIYINGGTFTITATG
                     TGGKGISADNNIEITNGSITISTSGGGAIYTNTSGKTDAYNATCLTSDGNIVIIDGTI
                     ALSSSGAGGKGVSSDGIFTLGSSSTSPTLSITTTGSEISLGNTSYSSPKALKADGDIK
                     FVGGTTLISSADDGIKSETNVYVNGGNIVVSKSYEAIEAPFIYINGGITDITATNDAI
                     NATKGLVSGGTEQNDGSCLYVTGGILIASCTNGDAIDSNGDILITGGLTIANGPSNGI
                     EEAVDFNGIFNMNGGIFIGAGSNSNMTKPMSNSSTQPNMFISSSSLISSSTFLNIQIN
                     STDVITFKPKYGGYKFLISSPDMTKGASYKIYTGGSYSTSINTGGYYTGGTYTPGNLK
                     KSGTLSLSSTVNTINL"
BASE COUNT          506 a          331 c          361 g          526 t
ORIGIN      
        1 aggtagacca gctgatgact gattttaaga atatagaaat tctaatcatt agtggactat
       61 acgctattga actcgaagaa gccgatctga aggtttttat cgaactcact tatagagaaa
      121 ctctcgatgt gcaacgtgaa tcggagaaag aagaactgga tgatttccgt atgaaggtgc
      181 tcgagcaaga acataaagcc gtgcaagcct taaaacataa agccgatttc tttgttgatt
      241 ttgatacggc tctcgaaatc tttcatcttt aagttgaaat tcactgaagg gcgaaggcat
      301 tagaagacat tatatatgtg cacggcgcag ggtattaaaa tgaggctgcg attaaatgaa
      361 tttcatccaa aatatcctta tatacgattt tgactaagta agaaggagtg gtgtatttaa
      421 ccaaaatata aaataggtat aaaaagagga acattctttt tattatctaa ttatctaaag
      481 ctgactggtg ctatttcata aattgatagt atttactgtg ctcgacaagg agagggttcc
      541 tgattttttt aagtttccag gtgtgtatgt tccgcctgta taatatcccc ctgtattgat
      601 agaagtcgaa taggaaccac ctgtatagat tttgtacgaa gcgcctttgg tcatgtcggg
      661 tgaggaaata agaaatttat atccaccata ttttggtttg aaggtaatga catcggtcga
      721 attgatttga atattcagaa atgtagaaga agagatcaat gagctcgaag agatgaacat
      781 attaggctga gtggatgaat tactcatagg ttttgtcatg ttggaattcg agcctgcacc
      841 aataaagatt ccgccattca tattaaatat gccgttaaaa tcaacggctt cttctatacc
      901 attagatggc ccattagcta tagttaggcc acctgttatc agaatatccc cgttgctgtc
      961 gattgcgtcg ccatttgtgc atgaggctat gagaattccc cccgtaacat acaaacaact
     1021 tccatcattt tgttccgttc cgccgctgac caggcctttg gtagcattta tggcatcatt
     1081 cgtagcagta atatcagtaa ttcctccgtt gatatagata aaaggcgcct cgatggcttc
     1141 gtaagattta ctgactacaa tattgccacc gttcacatat acattggttt ctgattttat
     1201 tccatcatct gctgaagaga taagtgttgt tccaccgacg aattttatat caccatctgc
     1261 tttaagcgct tttggtgatg aataacttgt gttccctaag ctaatttcac ttcccgtggt
     1321 ggtaatagaa agtgtgggcg aagtggaaga actccccaga gtaaagatcc catcgcttga
     1381 aacgcctttt cctcccgctc ccgacgaaga taaagcgatc gttccatcga ttatgacgat
     1441 atttccgtcc gacgtcaggc aagtagcatt gtaagcatct gtcttgccag aagtgttagt
     1501 atagatagct ccaccgcctg aggttgaaat tgtaatcgac ccgtttgtaa tttcaatatt
     1561 attatccgca gaaatacctt tgccacctgt ccctgtagcc gttatagtaa atgttccgcc
     1621 gttgatataa atcgaatgat tacatttgat ggctgtacaa taggagggat cataacccga
     1681 accggaagct tccaatatgg ctgcccccga ggtgttgatc gtga
//
LOCUS       DAORPD010000398         1720 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_13233_length_1720_cov_1.981381, whole genome shotgun sequence.
ACCESSION   DAORPD010000398 DAORPD010000000
VERSION     DAORPD010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13233_length_1720_cov_1.981381"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..1336
                     /locus_tag="PK976_05300"
                     /pseudo
     CDS             59..1336
                     /locus_tag="PK976_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842790.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
     gene            1535..>1720
                     /locus_tag="PK976_05305"
     CDS             1535..>1720
                     /locus_tag="PK976_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67336.1"
                     /translation="MDREKFKENAKKNIDDLFAKIDEMEAKKDKVKAETRAEYEAKIN
                     ELKAKKDELQKKYEALSE"
BASE COUNT          590 a          367 c          320 g          443 t
ORIGIN      
        1 ggaagtacgt taccaagggt agcgcagccc cagctgggag gaggttgagg ggaatgcttt
       61 tttgaataac gggcttacca ttctctttaa tggccagcga cttcaggcaa aaaacggatt
      121 ggccgatttg ctcgaagtaa acatggaaac cgaacctctt tatcccatca tccatctgaa
      181 agatgctgat tttgaatgcg ctttcacaca tgattcctca cattatggag aagagtatta
      241 ttcctttgta aacgggcagc ataccattca ggggggaacg caccagcagg cttttagaga
      301 agcttatgta aaaaccatcc gagatttctt caagaaagat tatgatacct ccgatattcg
      361 cactggtctt attgccgcta ttagcattaa aattcaggaa cccatcttcg aatctcaaac
      421 caagacacgc ctcggttcta cccttatgga acccgaagga caaagcgtca gcaagtatat
      481 tcatgatttt ctaaaaaaac atctcgacga ttatcttcat atcaatcctg ctacggccga
      541 agccattcag cgtaaaattc tgcaaagtga gaaggaacgc aaggaaatgg caggcattaa
      601 gaagcttgct cgcgaaagtg ccaagaaagt tagtcttcac aataaaaaat tacgcgattg
      661 tcgtattcat tacaattcga acgaccctcg caaactcgaa tccactcttt ttatcaccga
      721 aggagattca gcaagtggtt cgatcactaa aagccgcgac gtcaatactc aggccgtatt
      781 cagcctgaaa ggcaaaccct tgaacagcta tgggctttca aaaaaaatcg tttacgaaaa
      841 cgaagaattt aacctcttgc aatcagccct cgatatcgaa gatggtttgg aaaatctacg
      901 ttacaatcag attgtgattg ccaccgatgc cgacgtggat ggcatgcaca tccgactgct
      961 cctgctcaca ttctttcttc agtttttccc cgatctggta aaagccggac atttattcat
     1021 tttgcaaact cctttgttca gggtaagaaa caaacaacat acattttatt gttacaatga
     1081 agaagaaaag gaagaagcct tggctaaaac gggaaagaat cctgaaatca ccagattcaa
     1141 aggcttggga gaaatttctc ccgaagaatt tcgtcatttt attggcccac aaatgcgcct
     1201 cgaccccgta attcttcgca aagaccaagc tatcaacgaa attctcgcat attacatggg
     1261 aaaaaatact ccccaacgcc aggcattcat catagaaaac ctacgctacg aggccgatgc
     1321 tattttcata gattgattca tatccgttca tgagtatttt ccgaacagat caagaaaaat
     1381 caatttcttt atatcgaaat ttctattttg ttcgaaaaat cattcggaca atttttaaaa
     1441 aataaaattt actataattt tgtactacat tgcctgatat tatcaaataa tcatatatct
     1501 cattcactgt caaacattta aaaatataaa aattatggat agagaaaaat tcaaggaaaa
     1561 tgcaaaaaag aacatagacg atctctttgc caagatcgac gaaatggaag ccaaaaagga
     1621 caaggttaaa gctgaaactc gagccgaata cgaagctaag atcaacgaat tgaaggcaaa
     1681 aaaagacgag cttcaaaaaa aatatgaagc actaagcgaa
//
LOCUS       DAORPD010000399         1720 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_13234_length_1720_cov_1.842042, whole genome shotgun sequence.
ACCESSION   DAORPD010000399 DAORPD010000000
VERSION     DAORPD010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13234_length_1720_cov_1.842042"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="PK976_05310"
     CDS             <1..316
                     /locus_tag="PK976_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018358789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67337.1"
                     /translation="IDLHLAQDFYVNVAGKRNTLQITFDIFNLGNFINSDWGRKYYVS
                     GYYGNYPLLNFQGLKTDADGNKTVPTFTFQKPKGEVWNIDDSGLISSRWMGQVGIRYI
                     FN"
     gene            complement(464..563)
                     /gene="ffs"
                     /locus_tag="PK976_05315"
     ncRNA           complement(464..563)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PK976_05315"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            671..1234
                     /locus_tag="PK976_05320"
     CDS             671..1234
                     /locus_tag="PK976_05320"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HRC67338.1"
                     /translation="MKNKLILTLNNSLKYGALMGVVFILLSLIYYIAGFDQFNTVFGI
                     VNLLISLAMYIGFLYYSNKILRDKKLNGILTYTDGLLNSFFTGVIAAIVGGIYSYVFY
                     QYFDPSYFTEAIERSIEMLEQNPALTGEALDSAIARVESVTPLKSALQGFYGNIAMSL
                     IFALIISAFTRKKPIPSFSDEEISTHD"
     gene            1234..>1720
                     /locus_tag="PK976_05325"
     CDS             1234..>1720
                     /locus_tag="PK976_05325"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRC67339.1"
                     /translation="MDISIIIPLYNEAESLTELHAWITKVMQQNGFSYEIIFIDDGSN
                     DDSWKIIEALNHDDPHVKAIKFRRNYGKAAALNTGFEAAIGEVIITMDADLQDSPDEI
                     PELYQMIKNQGYDLVSGWKKKRYDPPSKTIPTKLYNWATRKATHIKLHDFNCGLKAYR
                     NE"
BASE COUNT          528 a          333 c          322 g          537 t
ORIGIN      
        1 cattgacctt catttggcac aggactttta tgttaacgta gccggcaaac gaaatacgct
       61 tcagattact ttcgatatct tcaatctggg taattttatt aattccgatt ggggtcgtaa
      121 atattatgta tcaggatatt atggtaatta tcctcttctt aacttccagg gactcaaaac
      181 cgatgccgat ggcaataaaa ccgtaccaac ctttactttc cagaaaccta aaggcgaagt
      241 atggaatatc gacgacagcg gtctgataag ctcacgatgg atggggcaag tcggaattcg
      301 ttatattttc aactaatatt tctatttcat tcgccatgaa aaaacctgct caaaagcagg
      361 ttttttcttc cgatgcatct catgcatctt tacaaatttc ttacggtgaa agagtgataa
      421 tagtattgtt cagcctcgct aagataaaaa tctataaaat aagaaagggt aagctcctta
      481 tatcgcaccg ctacgaccga ttacccttgc ttcctcccgg acctggggga gttcatcggg
      541 agctggtcgt ataagactta ccctttgcaa aaatacaaca aaattctaat tttttattct
      601 ttatcgaaag atttttacaa tttcgtgaat atttttacct ttgctcaata agtaaaaata
      661 cctttaaact atgaaaaaca aattaatcct tacgctcaac aatagtttga aatatggagc
      721 ccttatggga gttgttttca ttctcctcag cctgatatat tacattgccg gattcgacca
      781 gtttaatact gtttttggaa tagtaaatct tctaatatct ctggctatgt acattggatt
      841 tctttattac agtaataaaa tcctaagaga taaaaagctg aatggcattt tgacctacac
      901 cgatggtttg ctgaattctt tcttcacggg agttattgca gctatcgttg gtgggattta
      961 ttcttatgta ttttaccaat attttgatcc atcctatttt acagaagcta ttgaacgcag
     1021 tatcgagatg ctggaacaga atcctgcctt gacgggcgaa gctctggaca gtgctattgc
     1081 aagggtagaa agtgtaactc ctttaaaatc ggctttgcag ggattctatg ggaatattgc
     1141 catgagcctt atttttgcac tgattatttc tgcctttacc agaaaaaaac ccattcccag
     1201 tttttctgac gaagaaatca gtacccacga ttgatggata tatctatcat tattccttta
     1261 tataacgaag ctgaatcgct tactgaattg catgcctgga ttactaaggt aatgcagcaa
     1321 aacggatttt cttacgaaat tatctttatt gacgatggca gcaatgatga ttcatggaaa
     1381 atcatcgaag ctttgaatca tgatgatccg catgtaaaag ccatcaagtt tcgcaggaat
     1441 tatggtaaag ccgcagcatt aaatacgggt ttcgaagcgg caataggtga ggtaatcatc
     1501 actatggatg ccgatttgca agatagcccc gatgagatcc ccgagcttta ccaaatgata
     1561 aaaaatcaag gatatgattt ggtatcgggt tggaagaaaa aacgatatga tccgccttca
     1621 aaaactatcc ctacaaaact ttataactgg gctactcgca aagctacaca tatcaagtta
     1681 catgatttca actgcggatt gaaggcctac cgcaacgagg
//
LOCUS       DAORPD010000400         1718 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_13252_length_1718_cov_2.110042, whole genome shotgun sequence.
ACCESSION   DAORPD010000400 DAORPD010000000
VERSION     DAORPD010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13252_length_1718_cov_2.110042"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1718
                     /locus_tag="PK976_05330"
     CDS             <1..>1718
                     /locus_tag="PK976_05330"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRC67340.1"
                     /translation="IAKYNLGDYQGAEQDFTRSIEINPMFSEAYHYRAITRNRLRNYQ
                     AAMDDFKEALRIAPLSAEIYVNRSITRMGLKDYKNALIDVDSAIKIKNDIPLAYLARG
                     LIKHQLNDTLGAIADINKAIQLNYFFTEAYIRRGSIKYDIGDYKGAIDDFTQAIKMEP
                     DNPAVYFQRAIAWIKLENKNAAFDDLNKVLELDPYNALTYYNRAIIKGELGDLKGALE
                     DYNHVLALNPDNILAAYNRAITRHQLEDYHGAIEDYTKAINLFPDFSKAYFMRAEAKR
                     MLKDEKGAFEDRNKAIQIEETIAKTNDSILRLYTDSTYLASIIEFEADFRKTSAVDGR
                     IQYQPVYVDLEQNFIVTYLYNDSLYVEQKRGEYWLKKIDDIGKKVDYKLSFGLTNKET
                     KLTPEQIALYLKNIDLSTKLNPSDPLAYFFMGILNGMIKNYQSALDAYNKALELDPGF
                     SLAYFNRANTRLEMLEELKEQEPLPVAISFGNNMQQPRITEKTPLPEYQEVINDYNKV
                     IELNPDFSFAWFNRANIKARIADYESALQDYSIAIQLEPNLAQAYYNRALILIYQHNN
                     QPACQD"
BASE COUNT          578 a          408 c          302 g          430 t
ORIGIN      
        1 tattgcaaaa tataatcttg gcgattatca gggagcggaa caggatttta ctcgcagcat
       61 agaaatcaac cctatgttca gcgaagctta tcattacagg gctattacgc gcaacaggct
      121 tcgcaattat caggctgcta tggatgactt caaggaagct ctccgaatag cgcctcttag
      181 cgcagaaatt tatgtaaacc gaagcatcac cagaatgggt ttgaaagact ataaaaatgc
      241 tttgatagat gtcgattcgg ccatcaaaat caaaaacgac atccctctgg cttatcttgc
      301 ccgcggtctt atcaaacatc aactcaacga cactctggga gcaatcgccg acatcaacaa
      361 ggctattcaa ctcaattact tctttaccga agcttatata cgaagaggaa gcattaaata
      421 cgatattgga gattataagg gagctataga tgacttcact caggctatca aaatggaacc
      481 cgacaatcct gcagtttatt ttcaaagagc cattgcatgg attaaactcg aaaataaaaa
      541 tgccgccttt gacgatctga ataaagttct cgagctcgat ccatataatg cacttactta
      601 ttacaatcga gccatcatca aaggagaatt gggcgattta aaaggagcat tggaggatta
      661 caaccacgta cttgcactaa atcccgacaa tattctggca gcttacaacc gcgccattac
      721 tcggcatcaa ctcgaagatt atcatggagc catcgaagat tatactaaag ctatcaacct
      781 tttccccgat ttctcgaaag cctattttat gcgtgccgag gccaagcgaa tgctcaaaga
      841 tgaaaaagga gccttcgaag atcgcaataa agccatacaa attgaagaaa ccatcgctaa
      901 aaccaatgac tccatacttc gactctacac cgattctacc tatttagcca gcatcatcga
      961 attcgaagcc gattttagaa aaacttccgc cgtcgatgga agaattcaat atcaacctgt
     1021 ttatgtcgat ctcgaacaga atttcatcgt aacttatctt tacaacgact ccctatatgt
     1081 agaacaaaaa cgaggcgaat actggctcaa aaaaatcgac gacatcggta aaaaagtgga
     1141 ttataaactc agctttggcc ttaccaacaa agaaaccaag cttacacccg aacaaattgc
     1201 actttatctg aaaaatatcg acctgagcac aaaactcaac ccatccgatc cgcttgctta
     1261 cttttttatg ggtatattaa atggcatgat taaaaattac caaagtgctc tcgatgccta
     1321 taataaggcc ctggaactcg atcccggttt ttcactggcc tattttaatc gtgccaatac
     1381 tcgtttagag atgctcgaag aactgaagga acaagaacct ctacctgtgg caatttcctt
     1441 cggaaacaac atgcagcagc ctcgtattac agaaaaaacc ccactaccag aatatcagga
     1501 agttatcaac gattataaca aagtgatcga attgaacccc gactttagct ttgcatggtt
     1561 caaccgagct aacataaaag caagaattgc agattatgaa agcgcactcc aggattattc
     1621 catagccatc caactggaac ccaatctggc tcaggcttac tataatagag ctctcatcct
     1681 catctatcaa cataacaacc aacctgcctg ccaagacc
//
LOCUS       DAORPD010000401         1718 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_13256_length_1718_cov_1.918220, whole genome shotgun sequence.
ACCESSION   DAORPD010000401 DAORPD010000000
VERSION     DAORPD010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13256_length_1718_cov_1.918220"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /gene="rpsC"
                     /locus_tag="PK976_05335"
     CDS             <1..374
                     /gene="rpsC"
                     /locus_tag="PK976_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HRC67341.1"
                     /translation="NNVARDLERRISYRRAIKMAISNTMRIGAEGIKIQISGRLGGAE
                     IARREMFKEGRIPLHTLRADIDYARDEAHTTYGRIGIKVWICRGEIYGRPDFFATSSA
                     TRQQRSPVPGTSRPRSRGKRK"
     gene            407..829
                     /gene="rplP"
                     /locus_tag="PK976_05340"
     CDS             407..829
                     /gene="rplP"
                     /locus_tag="PK976_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HRC67342.1"
                     /translation="MLQPKRTKYRKMQKGRMKGNAQRGYEIAFGSFGLKALEQTFITG
                     RQIEAARQALTRHMKREGQIWIRIFPDKPITKKPAEVRMGKGKGAVEYYVAPVTPGRI
                     LFEADGIPLEIAREGMRLAAQKLPIKTKFVIRPDYLEE"
     gene            863..1072
                     /gene="rpmC"
                     /locus_tag="PK976_05345"
     CDS             863..1072
                     /gene="rpmC"
                     /locus_tag="PK976_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013546678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HRC67343.1"
                     /translation="MKQAVIKELSTNDLVERLEEERKKLTRLRLSHTVSPLDNPMKIR
                     QNRKTIARILTELKKRELEKNKKTS"
     gene            1076..1333
                     /gene="rpsQ"
                     /locus_tag="PK976_05350"
     CDS             1076..1333
                     /gene="rpsQ"
                     /locus_tag="PK976_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016660790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HRC67344.1"
                     /translation="METRNLRKERIGVVVSNKMDKTIVVKVERRLMHPKYGKFVKRST
                     KFKVHDQNNDCNVGDVVRIMETRPLSREKCWRLVEIIERAK"
     gene            1336..1704
                     /gene="rplN"
                     /locus_tag="PK976_05355"
     CDS             1336..1704
                     /gene="rplN"
                     /locus_tag="PK976_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HRC67345.1"
                     /translation="MIQQETRLNVADNSGAKEVLCIRILGGTGHRYATVGDKIVVTVK
                     KALPSGTIKKGTVTKAVVVRTKKEIRRADGSYIRFDDNACVILNNTGEMAGTRIFGPV
                     ARELREKQYMKIVSLAPEVL"
BASE COUNT          589 a          343 c          385 g          401 t
ORIGIN      
        1 ccaataatgt agcacgtgac cttgagcggc gtatatctta ccgcagggct atcaaaatgg
       61 caatttccaa taccatgaga attggggctg aaggcatcaa gattcaaata tcgggtcgtt
      121 taggtggtgc tgaaattgca cgtcgtgaaa tgttcaagga aggcagaatt ccactgcata
      181 cccttagagc cgatattgat tatgccagag acgaagccca tacaacttat ggacgcattg
      241 gcataaaagt atggatttgc cggggtgaaa tttatggaag gcctgatttt tttgccacat
      301 cgtcggctac ccgtcaacaa cgatcgcctg ttcccggaac tagccgcccc cgatccagag
      361 gtaaacgcaa ataatctatc agtaaaaaat taaaacctgt aatacaatgt tacagccaaa
      421 aagaactaaa tacagaaaaa tgcaaaaagg ccggatgaaa ggcaatgccc agagagggta
      481 tgaaattgct tttggatcat ttggcctcaa agcacttgaa caaaccttca ttaccggacg
      541 acaaatcgag gctgcccgcc aagccctcac acgtcacatg aaacgtgagg gacaaatctg
      601 gatacgtatt ttccccgaca aacctattac caaaaaacca gctgaagtac gtatgggtaa
      661 aggtaaagga gcagtagaat attatgttgc tcctgtaacc ccgggaagaa tccttttcga
      721 agccgatgga atcccactgg aaattgcccg tgaaggaatg cgactggcag ctcaaaaatt
      781 gcccatcaaa acgaaatttg ttatacgtcc cgattatctt gaagaataaa cctcaagctt
      841 tgtaaacata aaatttttaa agatgaaaca ggcagttata aaggaactct ccaccaacga
      901 ccttgttgaa aggctcgagg aagaaaggaa aaaactgacg cgtttgcgac tcagccatac
      961 agtttctcct ctcgataacc ccatgaagat tcgccaaaat cggaaaacga ttgctcgcat
     1021 tcttactgag ttgaaaaaac gtgaactcga aaagaataaa aaaacaagtt aaataatgga
     1081 aaccaggaat ttacgtaagg aacgaattgg agtggtagta agtaataaaa tggacaaaac
     1141 cattgttgtg aaagtcgagc gccgcttgat gcatcctaaa tatggaaaat ttgtaaagcg
     1201 aagcaccaaa ttcaaggtac atgatcaaaa taatgactgt aatgtgggtg atgtggttcg
     1261 gataatggaa acccgcccac ttagcagaga aaaatgctgg agactagttg aaattattga
     1321 aagagcaaaa taactatgat acaacaggaa actcgtttga atgttgccga taacagtgga
     1381 gccaaggaag tgctatgtat ccgtattttg ggcggtacag ggcaccgata tgctacggta
     1441 ggcgataaaa tcgtggttac cgtcaaaaaa gcccttcctt cgggtaccat caaaaaaggt
     1501 acagtaacca aggctgttgt tgtgagaacg aaaaaagaaa tcaggcgggc cgatggatca
     1561 tatatccgtt tcgacgacaa tgcctgtgtt attctgaaca ataccggtga aatggctggt
     1621 acgcgtattt ttggcccggt agcccgggaa ttacgtgaaa aacaatacat gaaaatcgta
     1681 tctcttgcac ctgaagtgtt ataatattga aatcaaat
//
LOCUS       DAORPD010000402         1708 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_13379_length_1708_cov_3.232305, whole genome shotgun sequence.
ACCESSION   DAORPD010000402 DAORPD010000000
VERSION     DAORPD010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13379_length_1708_cov_3.232305"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(401..>1708)
                     /locus_tag="PK976_05360"
     CDS             complement(401..>1708)
                     /locus_tag="PK976_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HRC67346.1"
                     /translation="GLTLIFSRKARAVTNTEVSLSRQEEGLERFSPNPFSRNIVRMLI
                     RFGNTTDKILPEGMKKFIASRLTPPDAEEMKQAHKAGISYDLVRASVNLVVSSALIAY
                     GTSHKLPLSTTYVTFMVAMGSSFADGAWGRESAVYRVSGVMTVIAGWFLTAFIAFSVA
                     FILATIIFFGKIPAIVILMILAALMLIHQQKVFVRKEKEAKIAQEILSDDSVISAEKM
                     IARCNNDVLSILKTTDRLYTRILVGLFKEDRKKLKAVQAEVDEFNKRTKLLKSNVHNV
                     VEKLKEDDVETGHFYVQVVDFMREIAHCINYISEPALNHVENNHKGLLPSQKAELTRI
                     SAALSELIHTLQHMIETCDLEQRDKIVKLQQQVNEYIDKARKDQVKRIKNHETGTKNS
                     LLYLNILAETKNLSLFILNLYKSHRDFVVFSIGHEMQILSDEK"
BASE COUNT          493 a          353 c          316 g          546 t
ORIGIN      
        1 tgttcttcaa gacgactaaa ttctcccagt cgtttgccta aatgccaatc agaagtatgc
       61 ataattttca tatcgggtgg cagtttgttt tttattcgaa gatattaaaa atcaaataga
      121 tatcatgatt caatagaata cacgcagaac acaaaattat gcattagtaa acaatatttt
      181 gaccctgctc ttatagccca tgcctcgcta tctcatcttt ccgaaaaact taaaataacc
      241 caaactatta tggtttttca cttcattgcg caatctctaa tcgccattct gtatcactgc
      301 tgtcgcacta cgtattcctc catcacgttc attttaataa cttatatacg tttaattcac
      361 tattctccgt tctggggaat gcataacagg aaacatgttc ctatttttca tcacttaaaa
      421 tttgcatttc atgtccaata gaaaaaacca caaaatctct atgcgactta taaagattga
      481 ggatgaaaag tgacaggttt tttgtttcgg ctaagatgtt gagatacaaa aggctattct
      541 tagttcctgt ttcatgattt tttatgcgtt ttacctgatc tttgcgggct ttgtcgatat
      601 attcatttac ttgctgctga agttttacta tcttgtcgcg ttgttcgagg tcgcaagttt
      661 ctatcatgtg ctgcaatgta tggataagct ctgaaagggc agccgaaatt cgtgtcaatt
      721 cggctttttg tgaagggagg aggcctttgt ggttgttttc gacatgattg agtgcgggtt
      781 cggagatata attgatgcaa tgtgcaattt cacgcataaa atctaccacc tgtacgtaga
      841 aatgtccggt ttccacatcg tcttccttaa gtttttccac tacattatga acattacttt
      901 tcagaagctt tgttcgttta ttgaattcgt caacttcagc ttgcacggct tttagttttt
      961 tacggtcttc tttgaataaa cccaccagaa ttcgagtgta taagcggtcg gtagttttaa
     1021 gaatcgaaag aacatcgttg ttgcagcgag caatcatttt ctcggctgaa ataacggaat
     1081 catccgaaag gatttcttga gctatttttg cttccttttc cttgcgaacg aagacttttt
     1141 gctgatgaat taacatcaaa gcagcgagaa tcatcagaat aacaatagca ggaattttac
     1201 caaagaagat aatagttgct aagataaaag ccacgctaaa tgcaataaat gccgtgagga
     1261 accaacctgc aataacagtc ataacgcccg aaaccctgta aaccgcactt tcccgacccc
     1321 atgctccgtc tgcaaacgac gaccccatgg ctaccataaa tgtaacataa gtagtgctca
     1381 agggaagttt atgggaagtg ccataagcaa tcaaggcact cgaaacaact aaatttaccg
     1441 aggctctcac aaggtcgtaa gaaattcctg ccttgtgtgc ctgtttcatt tcttcggcat
     1501 cgggaggagt aagtcgtgag gcaataaatt tcttcattcc ctctggaagt atcttatctg
     1561 tcgtattgcc aaatcggatg agcattctca caatatttct cgaaaaaggg ttaggagaaa
     1621 atctttccaa cccttcttct tgcctgctca aacttacttc ggtgttggtt acagctcgtg
     1681 ccttacgaga aaaaatgaga gtcaaacc
//
LOCUS       DAORPD010000403         1704 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_13435_length_1704_cov_2.238326, whole genome shotgun sequence.
ACCESSION   DAORPD010000403 DAORPD010000000
VERSION     DAORPD010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13435_length_1704_cov_2.238326"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="PK976_05365"
     CDS             <1..456
                     /locus_tag="PK976_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HRC67347.1"
                     /translation="VQKKVSHNDRPARIFDGFMNSGVWYVSGGRSYMAQLYKDAGLSY
                     VFSDLNETASKGLGFEEVFSRAHDADYWRIVVSQKENFSLQDLLNLDKRYVLFESARK
                     QKVVVCNASHVPLYEEGVAHPELILSDLVYLTRPELLPDYTPRYYQIIE"
     gene            456..1505
                     /locus_tag="PK976_05370"
     CDS             456..1505
                     /locus_tag="PK976_05370"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRC67348.1"
                     /translation="MHTLDISRHTPAFRIFIGIFLLLAFVILFFANLSVGTVKVSFSE
                     IMQIFAGKGRGLNQNALVVYEFRLPQALMAILAGASLAIGGLLMQALFRNPLADPSIL
                     GISSGASLGVALVTMAAGHISGVSIFSSGSWGYIGITLAALIGSLSVLMLLLFFSARL
                     KNIGQLLILGLMLGYLSYSLVSLLKFYSLKDDLQHYVIWGMGSFSEVSMEQLKIITPA
                     VMIGLVFSLLLAKSLNLMLLGDEYAQNLGLKLSLARTLIIFVAGFLTSIITAWCGPIA
                     FIGLAVPHLCRGLLATHDHHILLPVSMLTGAILSLICNFIARLPVFGGALPINAITSL
                     IGAPVMIGVILRLKN"
     gene            1519..>1704
                     /locus_tag="PK976_05375"
     CDS             1519..>1704
                     /locus_tag="PK976_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRC67349.1"
                     /translation="MKFNRRVFPIHEDALTTNHLSIGYLNPKTVIASDINLNFRRGRL
                     YCIIGPNGSGKTTLLRTL"
BASE COUNT          419 a          333 c          385 g          567 t
ORIGIN      
        1 gttcagaaga aagtttctca caatgatcga cctgcgcgta tattcgatgg attcatgaat
       61 tctggggtat ggtatgtttc gggaggaaga agttatatgg ctcaattata caaagatgcc
      121 ggtttgagtt atgttttttc cgacctgaac gaaaccgcaa gcaaaggcct tggtttcgaa
      181 gaggtattct caagagctca tgatgctgat tattggcgga tagtggttag ccagaaagaa
      241 aattttagcc tgcaggattt gttgaatctc gataaacgtt atgttctttt cgagtcagct
      301 cgaaaacaaa aagtcgtagt ttgtaatgct tcgcatgtgc ctctatatga agaaggagta
      361 gcccatcccg aactgattct ctccgatttg gtctatctta ctcgacctga acttctgcct
      421 gattatacac cccggtatta tcaaatcatc gagtaatgca taccttagac atctcacgtc
      481 atactccggc tttccgcatc tttatcggta tctttttact tctggctttt gtgatattgt
      541 tttttgctaa tctttcggtg ggtacagtaa aagtaagttt ttcggaaata atgcaaattt
      601 ttgcagggaa gggaagaggt ttgaatcaga atgctctggt agtatatgaa tttcgattac
      661 ctcaggcttt aatggccata ttggcagggg cttcactggc cattggaggc ctacttatgc
      721 aggctttgtt ccgcaatcct ttggccgatc cctccattct gggcatcagt tctggagcaa
      781 gtctgggtgt agccctcgta actatggcag ccggccatat tagcggagtt tccatatttt
      841 catcaggttc atggggatat attggcatta cgctggctgc tctaatcggc tctttgagtg
      901 tgttgatgct tcttctattt ttttcggcac gtctgaaaaa catcggccaa ctgcttattc
      961 ttggactgat gttgggttat ctgagctatt cgttggtgag tttgctcaaa ttttacagcc
     1021 tgaaagacga ccttcagcat tacgtgattt ggggaatggg aagcttttcg gaagtaagca
     1081 tggaacaatt gaagataatt actccagcag taatgattgg acttgttttt tcgcttttat
     1141 tagcaaaatc tctcaacctt atgctcttag gagatgaata tgcccagaac cttggattga
     1201 agctaagttt agctcgtacc cttatcatat ttgtggctgg atttttaacc tcgattataa
     1261 ctgcctggtg cggtcctata gcttttatcg gattggctgt tccacatctt tgtcgaggtc
     1321 ttctggcaac gcacgaccat catattttgt tacctgtgag catgctaaca ggcgcgattc
     1381 tttctttaat atgtaacttt attgctcgtt tgccagtgtt tggaggtgca ctgcccatta
     1441 atgccatcac ttcgctgatt ggcgctccag tgatgatcgg ggtgattctg cgtttaaaaa
     1501 attaaaatca atgtgtgaat gaaattcaac agaagagtct tccccattca tgaagatgct
     1561 ttaacaacga atcatcttag cattggatat ttgaatccaa agactgtaat tgcgtcggat
     1621 atcaatttaa atttcaggcg gggtcgtttg tattgtatta tcggacccaa tggttcaggc
     1681 aagactacat tgcttcgcac gttg
//
LOCUS       DAORPD010000404         1703 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_13440_length_1703_cov_3.490898, whole genome shotgun sequence.
ACCESSION   DAORPD010000404 DAORPD010000000
VERSION     DAORPD010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13440_length_1703_cov_3.490898"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            815..>1703
                     /locus_tag="PK976_05380"
     CDS             815..>1703
                     /locus_tag="PK976_05380"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008774267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HRC67350.1"
                     /translation="MKTRTEHDTMGPVEVPADKYWGAQTERSRQNFRIGPEGSMPKPI
                     IEAFAILKKAAALANYELGVLPEEKCKLIVTVCDEILDGKLDDQFPLVIWQTGSGTQS
                     NMNLNEVIANRAEVLRGGTLGTGKTFIHPNDDVNKSQSSNDTFPTAMSIAAYKTLVEI
                     TLPGVKKLRDTLRVKANEFKHIVKTGRTHLMDATPLTLGQEFSGYAAQLDYAIENIEA
                     AMPHVSELALGGTAVGTGLNAPKDYDVKVAANIAELTGYPFKTAPNKFEALSAHDALV
                     EASGALKTLAVSLMHIANNI"
BASE COUNT          561 a          327 c          343 g          472 t
ORIGIN      
        1 taatttggaa ccctgacgat tacaagtaag cgttgttcag gatatgaaca tcaagttgaa
       61 cttttaggca aatggtacaa ccaaatattg ctccgagggt ttaggatttt acggtgctgg
      121 atcaggcttt catgcagcct gttatatcat tctccagaac ttcctaccga tcaaggcaga
      181 ggttatgtgt taaaactatt cacgacaaaa aaataccatc tggtatttga ataaaatttc
      241 ttaagcaacg gaacgataaa agggaaaatt atgtccttaa tcaaaacata agtatctgat
      301 aatacacact ttaaaaataa ataggaaaat caatagttat tttgagactg tcgttacaaa
      361 aaagtaaacc tcaggaaggt tacactttca aaatttatga gaaagaagag acaaaatagg
      421 ataaaaaacg atcaatatcc acaaaaattg actcaaaaat tgaaatcttt ctaaccaaat
      481 tttttacttt cggattttaa ctatttacaa aaatcgagct aagataggca atgctgaata
      541 aagtcagttt cgttccatac aaatgaaagt aattgctgaa ttttttgcat gcgaagaatc
      601 aaggaattta aaatcaaagg aaatgatttt tgtgctcctt actctcatgg attcgtttat
      661 agaagattat ttcgaaatat tccatcagtg tctttcaatg gtttacgaaa agaacctatc
      721 aaatttatct gggcatgata tttttctctt ttctatatca agcaaaaatc tctaactttg
      781 agcttaatga gtttgcttta aaattttaaa aattatgaaa acacgtacag aacacgacac
      841 catgggacct gtagaagttc ctgccgataa atactgggga gctcagaccg aacgttcgcg
      901 tcagaacttc cgtattggcc ccgaaggctc tatgcccaag cccatcatag aggcttttgc
      961 catattgaaa aaggcggctg cactagccaa ttacgaactg ggtgttttgc ccgaagaaaa
     1021 atgcaaattg atagttacag tttgcgacga gattcttgat ggcaagctgg atgatcagtt
     1081 tccgctggta atctggcaaa ccggttcggg aacgcagagc aatatgaatt tgaacgaagt
     1141 cattgccaat cgagcagaag ttttgagagg aggtactttg ggcacaggga aaacctttat
     1201 acaccccaac gatgatgtaa acaagtcgca atcgtcgaac gacacttttc ccacggccat
     1261 gagcatagct gcttacaaga ctcttgtgga gattactttg ccaggcgtta agaaactacg
     1321 cgatacctta agggttaagg ccaatgaatt caaacacatt gtaaagactg gccgcacaca
     1381 tttaatggat gccacaccct taacgctggg tcaggaattt tcgggttatg cagcccaatt
     1441 agactatgcc attgagaaca tagaagcggc catgcctcat gtatctgagc ttgccttggg
     1501 aggaacggcc gtaggtacag gattgaatgc acccaaggat tatgatgtaa aggtagccgc
     1561 taatattgcc gagctgacgg gttatccctt caaaacggct cctaacaaat ttgaggcatt
     1621 atcggctcat gatgctttag tggaagcttc aggagctctt aagactcttg ccgttagcct
     1681 catgcacatt gcaaacaaca ttc
//
LOCUS       DAORPD010000405         1699 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_13496_length_1699_cov_2.519465, whole genome shotgun sequence.
ACCESSION   DAORPD010000405 DAORPD010000000
VERSION     DAORPD010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13496_length_1699_cov_2.519465"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1220
                     /locus_tag="PK976_05385"
     CDS             <1..1220
                     /locus_tag="PK976_05385"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRC67351.1"
                     /translation="LRYLLGKKTHHAINFVSGISVAGILTGTAALVIILSVFNGFQQV
                     IQSLYNVFDPDIKITAVKGKVFKPNEDIKNRISSMAGVAGISETIEDNALFRYRNHQH
                     IGLIKGVDQTYPLFSHLREKILQGEFVLSDSTGPYAVVGAGVAYYLNMGLDDAFYPLE
                     VFVPSRKSNLSALFSEEAFNSLAIRPSALFSVQQDFDISYVITPLSFARKLLEYSTEV
                     SAFEVYLKPNADIKSIQKKISEMMGSGFKVANRYEQQASLYKIMRTEKYAIFIILAFI
                     LIVATFTLIGSLSMVISEKQRDIAIFMAMGAPTSYIRKVFLYQGLWISVAGGIGGTLL
                     GAFICFLQQTFGFVKINTSGSSFVISQYPVEMQGMDFLVVFVFVVIIGIIASWIPARK
                     STQKSFIAYLRVE"
     gene            1342..>1699
                     /locus_tag="PK976_05390"
     CDS             1342..>1699
                     /locus_tag="PK976_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67352.1"
                     /translation="MKNKIFTQFSFLLLFLPLCMFSQQITIHPGGENAFKVLTSDYNA
                     FLFSNQLHTISWYSVSTSQGDFTEIAIPGYGFSNIVGCPKVPVIKKLIEVPLGAKTEV
                     KTIQVEYKEIPLEQYGI"
BASE COUNT          504 a          332 c          336 g          527 t
ORIGIN      
        1 ctttgaggta tttattaggc aaaaaaactc accatgccat taattttgtt tcggggatat
       61 ctgtagcagg catattaacc gggaccgctg ctctggttat catcctttcg gttttcaatg
      121 gctttcagca ggtaattcaa agtctctata acgttttcga tcccgatatt aaaatcacgg
      181 cagtgaaagg aaaggtattc aaacccaacg aagacatcaa gaacagaatt tcttctatgg
      241 caggcgttgc aggtataagt gaaaccatcg aagacaatgc tttattccga tatagaaatc
      301 atcaacatat tggtttgatc aaaggagtcg atcagaccta cccgctgttc tctcatctta
      361 gagaaaaaat tcttcaaggt gaattcgtac tgagcgattc aacgggtcct tatgcggttg
      421 tgggagcggg agtagcctat tatttgaata tgggtttaga cgatgcgttc tatcccctcg
      481 aggtatttgt tccttcccga aaatcgaatc tatcggcttt attttcggaa gaagccttca
      541 actcacttgc tatcagacct tctgcattgt ttagcgtaca acaggatttc gatatttcct
      601 acgtaattac tcccctttca tttgcgcgga agttactgga atattcaaca gaggtgagtg
      661 cttttgaagt ctatttaaaa ccaaatgccg acataaaatc tatacaaaag aagatttcgg
      721 agatgatggg ttcggggttc aaagttgcca atcgctacga acagcaagct tcgttatata
      781 aaatcatgcg aaccgaaaaa tatgctattt tcattatcct tgcctttatt ttgattgtgg
      841 ccacatttac gcttatcggt tcgctttcga tggttatttc cgaaaaacaa agagacatag
      901 ctattttcat ggccatggga gctcctacat cctatattcg gaaagtattt ctatatcaag
      961 gattatggat tagtgtagct ggaggcatag ggggtacact gctgggagcc ttcatttgct
     1021 ttttacagca aactttcgga ttcgtcaaga tcaacactag cggaagttca ttcgttatta
     1081 gtcaataccc cgttgaaatg cagggaatgg actttttagt ggttttcgtc ttcgttgtga
     1141 ttattgggat aatcgcctca tggattccag caagaaaaag tactcaaaaa tcattcattg
     1201 cttatctgag agtcgaataa catttcattt ctaaaaaaat gattttcatc gcatacatta
     1261 cattaacata ccgaagaaat agtactaatt ttgcgcaaga aattctcgaa gtgaactcaa
     1321 gaaatcaatt ccattttacc aatgaaaaac aaaattttta cacagtttag ctttttgctg
     1381 cttttcttgc ccttatgcat gtttagccag caaatcacca ttcatccagg gggagaaaat
     1441 gcttttaagg tgttaacgag cgattacaat gcttttttat tcagcaatca gctccacacc
     1501 atttcctggt attcggtgag cacttcgcaa ggagatttca ccgaaattgc catacctggt
     1561 tatggattca gcaacattgt tggttgtccc aaggtaccgg ttatcaaaaa attaatcgaa
     1621 gttccactgg gagccaaaac cgaagttaag acgattcagg ttgagtacaa ggaaataccc
     1681 ttagaacaat atgggatta
//
LOCUS       DAORPD010000406         1698 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_13508_length_1698_cov_2.284236, whole genome shotgun sequence.
ACCESSION   DAORPD010000406 DAORPD010000000
VERSION     DAORPD010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13508_length_1698_cov_2.284236"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1634
                     /locus_tag="PK976_05395"
     CDS             <1..1634
                     /locus_tag="PK976_05395"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HRC67353.1"
                     /translation="SIPQGNYTVRYSAIGYITQDISDITINNGQTTTQDVTLEPAGNP
                     PTNLIATQGAGLQVDLSWEASTSPGVVGYNIYRKQYSFEYYPSTPLGFVEAGKLSFAD
                     STALPLIHYYYVVTAKLSGDLQTPYSNEAIGWISTGFITNEISAYYGSTPSIDGIISP
                     GEWSDAFMVDLSNVLGRYDNIVRPIGSVMGYFKVNENKTALYVAVDNKNDVSLKDHDE
                     VALYIDDNNDGSYPEPGDDSEGNYWAVYYGSGNLIKYRPIYNTGGVGTTIELVNPQIE
                     VSDLSGHVVYEFVLPLGHDYPWQINYNANNESGLFVFVLDDPSYYTGWWPCQNMNIFT
                     PEGYGKITFGAIDELPPPPENLQIEVGFGGKIMLHWTQPTINDFNHFNIYWLAEGSPN
                     FTLLDTTVGVQYFYTVPSSGLYKFYVTTVDNTGHESVPSNIVQANIIIGIDEFILNED
                     FSSIKIWPNPFEGILNIDIEAMKETNLSIRITDMSGKLIANVFDSRISKGYHHFTWEG
                     KMDNGKLLKPGVYLINLMSETGYIQTYKLVVMGRK"
BASE COUNT          521 a          292 c          357 g          528 t
ORIGIN      
        1 cgagtattcc tcaaggaaac tatacggttc gctacagcgc aatcggatat attacgcagg
       61 atattagtga tatcacaata aataatggcc aaaccaccac acaggatgta acactcgaac
      121 cagccgggaa tccgccaact aatctgattg ctactcaggg agcgggtctt caggtagatt
      181 taagctggga agcttctaca tcgccaggag ttgttggata taatatttac aggaaacaat
      241 attctttcga atattacccc tccacaccat tgggatttgt agaagcaggg aaactctcgt
      301 ttgccgatag tacggcattg cctttgattc attattacta tgtggtaact gcaaaacttt
      361 caggagattt gcaaactcct tattcgaatg aagctatagg ttggatttca actggtttta
      421 ttacaaatga gattagtgcc tattatggat cgactccttc gatcgacggc attattagtc
      481 cgggtgaatg gtctgacgct tttatggttg atttgtcgaa tgtattgggg aggtatgaca
      541 acatcgtcag gcctatcggc agcgtgatgg gctattttaa agtaaatgaa aataaaacgg
      601 cactttatgt tgcagtagat aataaaaatg atgtatcgct aaaagatcac gatgaagttg
      661 ctctatacat cgacgacaac aacgatggtt cttatcctga acctggcgat gattcggaag
      721 gtaattactg ggctgtttac tatggctcgg gtaatctgat taaatacaga cccatatata
      781 atactggagg tgttggtact acgatagaac ttgtaaatcc acaaatcgaa gtttcggatt
      841 taagtggaca tgtagtttat gaatttgttt tgccgttagg gcatgattat ccatggcaaa
      901 tcaactacaa tgcgaataat gagagcgggt tatttgtgtt tgttcttgac gatccatcgt
      961 attataccgg ctggtggcct tgccagaata tgaatatttt tactcccgaa ggctatggta
     1021 aaataacttt tggcgctatt gacgagcttc ctcctccccc tgaaaattta cagattgaag
     1081 taggattcgg aggaaagatt atgttgcatt ggactcaacc tactattaac gacttcaacc
     1141 atttcaacat ctattggcta gctgagggaa gtcctaattt tacattgctt gatacaactg
     1201 tgggtgtaca atatttttat acggttcctt cgagtggtct atataaattc tatgtaacaa
     1261 ctgtagataa tacgggtcat gaatcggttc cttccaatat cgttcaggct aatattatca
     1321 ttggaatcga tgaatttata ttgaatgagg atttttcatc cattaaaatc tggcctaacc
     1381 ctttcgaagg aattttaaat atcgatatag aagccatgaa agaaacaaac ctttcgattc
     1441 gaataacgga tatgagtggt aaattaatcg ccaatgtatt tgattcgcga atttcgaagg
     1501 gatatcatca ttttacatgg gagggtaaaa tggataatgg gaaacttttg aaacctggcg
     1561 tttatctgat taatctaatg tcagaaactg gatatatcca aacctataaa ttggttgtaa
     1621 tgggaagaaa gtaatttaga taaacattag aaaatttcga aatcattgaa gaagggatat
     1681 cgatatttat cccttctt
//
LOCUS       DAORPD010000407         1689 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_13628_length_1689_cov_2.137087, whole genome shotgun sequence.
ACCESSION   DAORPD010000407 DAORPD010000000
VERSION     DAORPD010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13628_length_1689_cov_2.137087"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..549
                     /locus_tag="PK976_05400"
     CDS             97..549
                     /locus_tag="PK976_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67354.1"
                     /translation="MSAILKKIAKEPLYVINLAGQKEPFSWEKVFLSAQRAGSSKELA
                     KKIADEVSLLAYPGISTALIHEEVLKRLRENEPRAGIRFSLKKAIQRLGPTGFPFEKY
                     IGALFENQGWQVSLNQHLKGYCLTYEIDFLAEKTIVPFFLPLPFEFPL"
     gene            complement(1172..>1689)
                     /locus_tag="PK976_05405"
     CDS             complement(1172..>1689)
                     /locus_tag="PK976_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67355.1"
                     /translation="FSNLYLTTYDGKVLELQPGDYPEEEPFTLHLYQELSPVPPRVAS
                     KLNPRQFMNYVTDQRNPVSFPKVVFAELILNGLAENPITGMSNNLPYQNINHLRDCLI
                     GLYSGYEKPNKTVERFYYGDLLYRTVKNGFFLGDQDHFVYYPFPPVDQLQKLYYPWWR
                     SALNLEFNEKK"
BASE COUNT          571 a          315 c          313 g          490 t
ORIGIN      
        1 aaatgtttct ctcgcttagg cattctatcc ctaaatcaat aaatcaaccc ccggggttgg
       61 cgagccttcg aacctcgaga aaaatttaat atttagttga gtgctatact taaaaagata
      121 gctaaagaac ctctttatgt tatcaatctt gccggtcaaa aagagccttt ctcttgggaa
      181 aaagtttttt taagcgcgca aagggccggc agttctaaag agttggccaa aaaaattgcc
      241 gacgaggtca gtcttttggc ttacccgggc atctccaccg ctctcattca cgaagaagtc
      301 ctaaaacgtc ttcgcgaaaa tgaacccagg gcgggaatcc gattttctct taaaaaagca
      361 atccagcgtc ttggccccac cggtttccct tttgaaaaat acatcggcgc tcttttcgaa
      421 aatcagggct ggcaggtttc tctcaatcag catcttaaag gctactgtct tacctacgaa
      481 attgattttt tagccgaaaa aacaattgtg ccattttttc ttccgcttcc tttcgaattt
      541 cctttataaa ttctacatct aacatggtgg gagtaataat agaatcggga gaatacccct
      601 caggcatgat aaattgatcg aaaaccgcat gaaacaacaa aatttcggct cccgtagcct
      661 ttgccatcga gaaggcatat gaggctgtaa caaaagtatg gtcggaaaaa tcaacaggaa
      721 caagaatacg tttcatatct caaaggtatt ttcgaatgat tgaaaaactt ctacaaatat
      781 aaaccaaaaa ggaatccatt gcgtgaactt ggtaaaaaat tatatggcaa tatccaagaa
      841 acccttaatt ttttcgaaaa taaaacttta tcaacaaaaa aatattgata aattctgaaa
      901 ctatttaatt tgtcggattt tcgatactca aaaaaataaa tgcaatacaa taattctaat
      961 cattacaagt aaggcacaat aaaaaatcgc aatccaaaag tcgtatgatt tttatttttt
     1021 atatatgata aaaaagcctt ttcaatgcat ggtttttcat acgaaaatga atataagaca
     1081 acagcatcat tggtaattcg ataaaatcgt taatatattc atccttgctt gatggaatgc
     1141 agagagaatt aatgaatttc aatatatcaa tctatttttt ctcgttaaat tcgagattaa
     1201 gggccgaacg ccaccacgga taataaagtt tttgtaattg atcgaccgga gggaaaggat
     1261 aatatacgaa atgatcctga tcaccaagaa aaaatccatt tttaacggta cgatagagca
     1321 aatctccata ataaaaacgt tcgacggttt tgttgggttt ttcgtagcct gaatataaac
     1381 cgatgaggca gtcgcgtaaa tgattgatat tttgataagg gagattgttc gacatgccag
     1441 tgataggatt ttcggccaat ccattgagaa taagctcagc aaataccact tttgggaaag
     1501 agactggatt cctctgatca gttacataat tcatgaattg cctgggattt agcttgctgg
     1561 ctacacgagg aggtaccgga gacaattcct gataaagatg aagcgtgaaa ggttcttctt
     1621 cagggtaatc tccaggctga agttctaaaa ccttcccgtc ataggtagta agatagaggt
     1681 tgctgaaaa
//
LOCUS       DAORPD010000408         1686 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_13661_length_1686_cov_2.320049, whole genome shotgun sequence.
ACCESSION   DAORPD010000408 DAORPD010000000
VERSION     DAORPD010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13661_length_1686_cov_2.320049"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(340..594)
                     /locus_tag="PK976_05410"
     CDS             complement(340..594)
                     /locus_tag="PK976_05410"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HRC67356.1"
                     /translation="MENLSSIFSSISFGLIAGISIGPTFFLILDHTITQSRTQGFLSA
                     AGVILSDVLIFILAGIGVAQFIEQDNVFFTSDWEGLSCWL"
     gene            703..1599
                     /locus_tag="PK976_05415"
     CDS             703..1599
                     /locus_tag="PK976_05415"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRC67357.1"
                     /translation="MKKPQHIIWCIGLLLFLLSSLKAQIPSQGFSFVFMTDIHLTRDD
                     YAVEGFRKALAVASDLKPDFIITGGDLVMDALDCDYQHAIDQYDLYLGTISNSPIPFY
                     NAMGNHEIWGWQNKHLRQEEWEDYGKNLFKRRIGPPNYSFDHKGWHFVVINGIEYNDS
                     TSYVGWALDETLDFIQNDLKNVPVGMPIILITHIPLITLEAQWEKGALACNHPMEVIG
                     NSKEVLECFPAHSLRLVLQGHLHYYEKMEIDGIIFITGGAVCGAWWKGNYRVTDEGFV
                     LIRINGEYCQTQYIDYGWEAMR"
BASE COUNT          527 a          294 c          337 g          528 t
ORIGIN      
        1 atgaataaat agatgcctga tgctatcagc actccgccta ctgaaacatt taaaattctt
       61 ctccattttc cttttgatat ccgacttaaa taatgcgcta aaaaaccttt attgccatta
      121 aaagactctc tgttagaaaa atggaaagga aaacgacaaa atgggaagaa tcattcgcga
      181 tgctatttgc cagagagatg ccctgtgcca ccatccaata taaccaattg aatgggttga
      241 gggcatttac aaaaaaagtt tgtaacatgg gcatcgaact ataagaatgg ccggctgata
      301 aatgtttttt ctagatgtct attgatgaaa ataagccaat tataaccagc aagatagacc
      361 ctcccaatcc gaagtaaaaa agacgttgtc ttgttcaatg aattgagcca ccccaattcc
      421 agctaaaatg aaaatgagaa catcagaaag aatgacacca gcggccgata aaaatccctg
      481 cgttcgactc tgagtaattg tatgatcgag aatgagaaaa aaagttggtc ctatgcttat
      541 gccggctatc aatccaaaag aaatcgacga aaatatgctc gataaatttt ccatactttt
      601 attcacaaac ttacttcaat cttattcttc ttgaaaattt aaaaagattt taatcctctt
      661 tttaattgtg tttgaataga tttgcataat aaattcatca tcatgaaaaa accgcaacac
      721 attatatggt gtatcggtct tttactgttc ttattgagca gcttaaaggc gcagattccc
      781 tcccaaggat tttcgtttgt gtttatgacg gacattcatc tcacgagaga tgactatgcc
      841 gtagaagggt ttagaaaggc attggctgtc gcttctgatt taaaacccga ttttattatt
      901 actggtggag atttggtgat ggatgccctt gattgtgatt atcagcatgc tatcgatcaa
      961 tatgatcttt atttaggaac gatatccaat tcccctattc ctttttacaa tgcgatggga
     1021 aatcatgaaa tttggggctg gcaaaacaaa catctccgtc aggaagaatg ggaggattat
     1081 gggaaaaatc ttttcaaaag aagaattggt ccaccaaatt attcgttcga ccataagggt
     1141 tggcatttcg ttgttattaa tggaatcgag tacaatgatt cgacgagtta tgtaggttgg
     1201 gctttagatg aaactctcga tttcattcag aatgatttga aaaatgttcc ggttggcatg
     1261 ccaattattc tcataacgca tattcctttg attacgctcg aagctcaatg ggagaaaggt
     1321 gcactggcct gtaatcatcc tatggaagtc attggaaatt cgaaggaggt tttggaatgt
     1381 ttccctgctc atagcctgcg attagtttta caaggccatt tgcattatta tgaaaaaatg
     1441 gaaatcgatg gaattatttt tattacagga ggtgctgtat gtggagcctg gtggaaagga
     1501 aattaccgtg ttaccgacga aggttttgtg ctcatcagga taaatgggga atattgccag
     1561 acgcagtaca tcgattatgg ttgggaagct atgagataaa ttttctctaa tttttttaca
     1621 attggtaaaa tgataataaa ttcctgtgtt tacgggtttt tcccgtgtct gtatctcttc
     1681 ccttgc
//
LOCUS       DAORPD010000409         1684 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_13693_length_1684_cov_2.147330, whole genome shotgun sequence.
ACCESSION   DAORPD010000409 DAORPD010000000
VERSION     DAORPD010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13693_length_1684_cov_2.147330"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1684)
                     /locus_tag="PK976_05420"
     CDS             complement(<1..>1684)
                     /locus_tag="PK976_05420"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HRC67358.1"
                     /translation="IYKIGDHGLNPYLRKTPQIKIQQEFSQVYQKQFLNYNPTDYTPL
                     PDFGKMLVICYPDFMASMQPYVDWKNSIGIPTEMVNVATIGSTSAVIKNYIVNYYNTN
                     TLTFVLLVGDGPQIPTNTGSGLGGPSDNAYGYIVGNDHYIDVFIGRFSAENVAHVQTQ
                     VSRTLEYEKNPSFLNDDWYTTCLGIASDQGPGDDGEYDYQHVRNMQTDLLGYTYTWNP
                     ELFDGSQGGNDAPGNPTPAMVSSEVNNGTSIILYTGHGSTTSWGTSGFSNANVNQLTN
                     VGKLPFIWAVACVNGEFMNTTCFAEAWLRATQNNQPTGAISFLGSTINQSWNSPMEGQ
                     DEMVDILVETYPNNIRRTFAGLSLNGCAKMIDSYGTDGSNMADTWTVFGDPSLMVRTA
                     NPQPLSATFNPTIFVGASTFTINCNVNGARVTLSNNGTILNTTWVANGTATLNFTPLI
                     TPNDTLTLTVVEYNYIPLIAQIPVIPAVGPYVIYESTQIADFTTGNGNGLLDYSESSN
                     LTISVKNIGVDSSVNTWVSLRSLSPYVEITDSIENYGTIPAGEIISVDEGFQ"
BASE COUNT          477 a          333 c          381 g          493 t
ORIGIN      
        1 aattgaaatc cttcatctac gctaataatc tcgcctgcag gtatagtgcc atagttttca
       61 atcgaatcgg taatttcgac ataagggcta agagagcgta aacttaccca tgtattgaca
      121 gatgaatcca ccccgatgtt tttaacggag atagtcaggt tagatgattc ggaataatcg
      181 agcaaaccat taccattgcc tgttgtaaaa tcagctattt gggtgctttc ataaataaca
      241 tagggaccta ctgctggaat aacggggatt tgtgcaatta ggggaatata attatattcg
      301 actacagtaa gtgtaagcgt atcattgggg gtaatcaaag gcgtaaaatt aagagtagcc
      361 gttccgttgg ctacccaagt ggtattgaga atagtgccat tattcgaaag agtaacccgg
      421 gcgccattta cattgcaatt gatagtgaaa gtgcttgctc caacgaagat agtaggattg
      481 aaggttgctg aaagaggctg aggatttgcg gttctgacca ttaagctggg atcgccaaat
      541 actgtccatg tatcggccat attagaacca tcggttccgt aagaatctat cattttggca
      601 caaccattca aagataagcc agcaaaagtc cttctgatat tgttaggata ggtttctacg
      661 agtatgtcca ccatctcgtc ttgtccttcc ataggtgaat tccagctttg gttgatagta
      721 gaaccaagaa atgagatagc acccgttggc tggttatttt gagttgctct gagccatgcc
      781 tctgcaaaac aggtagtatt cataaattcg ccgtttacgc aagctactgc ccaaataaaa
      841 ggcaatttac ctacattggt gagctgattt acattggcat tgctgaatcc cgatgttccc
      901 catgaggtag tacttccatg accggtatag agtataatgc tggttccatt gtttacctcg
      961 cttgaaacca tagctggagt tggatttcct ggagcatcat taccaccctg cgaaccatcg
     1021 aataattccg gattccatgt ataggtataa cctaacagat ccgtttgcat atttcttacg
     1081 tgctgataat catattctcc gtcgtcacca ggtccttggt cggaggctat gccaaggcaa
     1141 gtagtatacc agtcatcatt aaggaaggaa ggattctttt cgtattcgag ggtgcggcta
     1201 acttgagttt gtacatgagc cacattttcg gcagaaaaac gaccgataaa tacatcgata
     1261 taatggtcgt tgcccacgat gtatccatag gcattatcgc tgggtcctcc caacccactt
     1321 cctgtattgg taggaatttg aggcccatcg cctaccagca gtacgaaagt tagagtattg
     1381 gtgttataat aattaacgat ataattctta attacagccg aagtactgcc aatagttgca
     1441 acattcacca tttcggtagg gatgccaatg gagttcttcc agtcgacata aggttgcatg
     1501 ctggccataa aatcgggata acaaatgacg agcatcttcc cgaagtcggg taggggggta
     1561 taatcggtag gattgtaatt gaggaattgt ttttggtaaa cttgtgagaa ttcctgctgg
     1621 attttaatct ggggtgtttt ccgaaggtaa ggattcaatc catggtcgcc aattttataa
     1681 atct
//
LOCUS       DAORPD010000410         1684 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_13696_length_1684_cov_1.993247, whole genome shotgun sequence.
ACCESSION   DAORPD010000410 DAORPD010000000
VERSION     DAORPD010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13696_length_1684_cov_1.993247"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1684)
                     /locus_tag="PK976_05425"
     CDS             complement(<1..>1684)
                     /locus_tag="PK976_05425"
                     /inference="COORDINATES: protein motif:HMM:NF016563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl hydrolase"
                     /protein_id="HRC67359.1"
                     /translation="VGGYILFSEDNINRHHLEKFTSNCLYYLNNSDEYRPIPAFYWNN
                     AQTSIKSFRLKDTLHLKEKTYSHFLAETSSQPKVSKNANGQYYQVSGQRIVLMGKENG
                     GIKEIWAHPFMAMYDYKVILQAPSPALPVYLDSLIPEITIRPGCIKRHYQLQNNSLNE
                     IIVAHPEKGIATIHYEFEGDEKTHLYITYQSNFRLMWPYSEKASRILRYSKGNKNRFH
                     IITDFTQDYVCLTGSQRIPHQTKAGHFNGFTFDEQARIIGHVTDEAIASCCYDYLLKS
                     KDTLDFLIIAGSEGLQAALDNFSSTAKNSARLSSEHEKAMKNLYEYHLQIETPDTLFN
                     EAWKWYLAGINSFYIETPQLGRSLVAGFNWTGTGWNGGHRIDGRPGYSWYFGRDSEWS
                     ALAILDYGDFEKAKDVLSMLSDYQDLNGKIFHELTTSGFVHYDAADATPLYIVLACRY
                     LRHTGDTAFIRSIWPNLLQAYKFCKSTDTDYDHLIENTNVGHGWVEGGPLYGSHTSLY
                     LASCWNEALIAMAEMASVMNEDSLAHECSKEAYIVNHLLNDSFYDTHEQSWRQ"
BASE COUNT          473 a          348 c          337 g          526 t
ORIGIN      
        1 cctgacgcca cgattgctca tgcgtatcgt aaaagctatc gttcaagaga tggttcacaa
       61 tataagcttc cttgctgcat tcgtgggcga ggctatcctc gttcatcact gatgccattt
      121 cagccatagc aattagagct tcattccagc acgaagccag ataaagtgaa gtatgagaac
      181 catataatgg gccgccttct acccaaccat gtccaacatt ggtgttctcg atcaaatgat
      241 cgtaatcagt atcggtagat ttgcaaaatt tataggcttg gagtaagtta ggccatattg
      301 aacggataaa agccgtatct cccgtatggc gaagatatcg gcaagccaga actatatata
      361 aaggagtggc atcagcagca tcgtaatgga caaaaccgct ggtagttagt tcatgaaaga
      421 ttttaccgtt gaggtcttga taatcgctta acatgcttaa gacatccttt gccttttcaa
      481 aatcgccata gtctaaaatg gctaaagccg accattcgct atctcttcca aagtaccaac
      541 tatatccagg tcttccatct atgcgatggc ctccattcca gccggtccct gtccaattga
      601 agcctgctac cagtgacctt cccaactgtg gcgtttctat gtagaaactg tttattcctg
      661 ccaaatacca tttccatgct tcattaaaaa gtgtatctgg tgtttcaatc tgaagatgat
      721 attcgtaaag atttttcatc gctttttcgt gctcgctcga taaccgagca gaattttttg
      781 ctgttgaaga aaaattgtcg agggctgcct gtaatccttc gcttccggca ataattagaa
      841 agtcgagggt atctttgctt ttgagtaaat agtcatagca acacgatgct atcgcctcat
      901 cagtaacatg gccaataatt cttgcctgtt catcaaaggt gaagccatta aaatgaccgg
      961 ctttcgtttg atgaggaatt ctttgtgaac ctgtcaaaca gacatagtcc tgtgtaaaat
     1021 ctgtaattat gtgaaatctg tttttgtttc ccttgctgta acgcaaaatc ctcgaagctt
     1081 tttcgctgta gggccacatc aaacgaaaat tgctctgata agttatatac agatgagttt
     1141 tttcatcccc ttcgaattca taatgaattg ttgcaatgcc cttttcggga tgggctacga
     1201 tgatttcatt caaggagtta ttctgcagtt gatagtgtcg ttttatacaa ccaggtcgaa
     1261 tggtgatctc gggtattaaa ctgtccagat aaacaggcag agcaggtgaa ggcgcttgta
     1321 aaatgacttt ataatcgtac attgccataa aaggatgagc ccaaatttct tttatcccac
     1381 cattttcttt tcccattaat acgattcttt gcccagaaac ctgataatat tggccatttg
     1441 cgtttttact tactttaggt tgtgaggatg tctcggccag aaaatgagaa taggtttttt
     1501 ctttgagatg caaggtatct ttaagacgaa atgactttat cgaagtttgg gcgttattcc
     1561 aataaaaggc cggaatcgga cgatattcat ccgaattgtt caaataatac agacaattcg
     1621 atgtaaattt ttcgagatga tggcgattga tgttgtcttc cgaaaataat atgtatcctc
     1681 ccac
//
LOCUS       DAORPD010000411         1681 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_13731_length_1681_cov_2.258303, whole genome shotgun sequence.
ACCESSION   DAORPD010000411 DAORPD010000000
VERSION     DAORPD010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13731_length_1681_cov_2.258303"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="PK976_05430"
     CDS             complement(<1..353)
                     /locus_tag="PK976_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67360.1"
                     /translation="MTYKNITRLFFVFALVFHVFSVSAQRRILHWETLQTTTDTTLNL
                     WLQEAAYINDSLNVPVYLEEIPLPVGSNGLEYNLKEVYFEKLESKISGIGTEIKINVT
                     IVTECKQKKARITFIP"
     gene            complement(382..>1681)
                     /gene="porU"
                     /locus_tag="PK976_05435"
     CDS             complement(382..>1681)
                     /gene="porU"
                     /locus_tag="PK976_05435"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HRC67361.1"
                     /translation="IALFTTSRLTYGGSNITLNQNFYKYAFERTNGQYRTLGEIIMIA
                     KQKSGSDLNWIKFILLGDPALKLAYPENKVSALTINNTSIEISTDTLKALSNVSITGE
                     VTDPLSYPLHKFNGTVTTTIFDKVSLIKTKANDPESKPTTFALRKNIIYKGLSEVKNG
                     KFYMNFIVPKDIEYKIGEGKICFYACSDKDDAAGYFAPVYVGGINTNAQIDNTPPSIH
                     LFMNDLNFKDGGITDENPVLLAFISDENGINTVGSGIGHDIVAILDNNSNNPLILNNY
                     YKSDLNTYTSGSIAYPLFNLEEGDHEIRLTAWDVYNNSATSKLKFTVKNSASFIISNL
                     YNYPNPFYDKTYFVFEHNKSSEEMQVEIRIMDIYGRVVKTIAHTIFFTGYCSQPIEWD
                     GASDNGKRLSQGIYIYQLTLTDSKGNARQKSGKLMISKNQ"
BASE COUNT          479 a          294 c          353 g          555 t
ORIGIN      
        1 ggaataaaag ttatccttgc ttttttctgc ttgcattcgg taacgatagt tacattgatt
       61 tttatctcgg ttcctatccc actaattttc gattcgagtt tttcgaaata cacttccttc
      121 agattatatt cgaggccgtt gctcccaact ggaagaggta tttcttccag ataaacggga
      181 acattcaatg agtcatttat ataggcagcc tcttgcaacc ataaattcaa ggtagtgtca
      241 gtagttgttt gtaatgtttc ccaatgtaga atccttcgtt gagctgagac ggagaaaaca
      301 tgaaaaacaa gagcaaagac gaagaacagg cgagttatgt ttttataagt catcgaaaat
      361 atggtgtatt atgagcaaag tttattgatt tttcgaaatc atgagtttgc cgcttttttg
      421 tcgagcgttt cctttcgagt cggttaacgt caattgataa atataaattc cctggcttag
      481 tctttttccg ttgtccgagg caccatccca ctcgatgggt tggctacagt atcctgtaaa
      541 gaaaatagta tgagcaatag ttttgacaac ccggccataa atatccatta tccttatttc
      601 gacctgcatt tcttctgagc tcttattatg ctcaaaaaca aaataagttt tatcataaaa
      661 agggttagga taattatata gattcgaaat aatgaaggaa gcagaatttt tcacggtgaa
      721 tttcagtttg gatgtggccg aattgttata aacgtcccat gccgtgaggc gtatttcgtg
      781 atccccttct tcgagattaa acaagggata agcaatactt ccactggtgt aggtatttag
      841 atctgatttg taataattat tcaggatgag gggattgttc gaattgttgt cgaggatggc
      901 cacaatatcg tggccaatgc cgctacctac ggtatttatt ccgttttcat cggaaataaa
      961 ggcgagcagg acagggtttt catcggtaat tcctccatct ttgaaattca gatcattcat
     1021 aaataaatga atagagggag gtgtattgtc gatttgggcg ttggtattga ttccaccaac
     1081 ataaacaggt gcgaagtaac cggccgcatc atccttgtcg ctgcatgcat aaaagcaaat
     1141 tttgccttcg ccaattttgt attcaatgtc ttttggtacg ataaaattca tgtaaaactt
     1201 accattttta acttccgata aacctttata aatgatattc tttctcaatg caaaggtagt
     1261 aggtttactt tccgggtcat ttgccttggt tttgatgagc gagaccttat cgaaaatggt
     1321 ggtcgtaacg gttccattga atttatgtaa tggatagctt agaggatcgg taacttcgcc
     1381 agttatggaa acattcgaca gagccttgag ggtgtcggta gaaatttcga tcgaagtatt
     1441 gttgatagta agtgcactaa ctttattctc gggataggca agttttagtg ctggatcacc
     1501 gagcaaaatg aattttatcc aattcaggtc ggaaccgctt ttttgcttgg caatcatgat
     1561 aatctcgccc aaggtacggt attggccatt cgttcgttcg aatgcatatt tgtaaaaatt
     1621 ttggttaaga gttatattag aaccaccgta ggtcaatcgt gatgtagtaa ataatgcaat
     1681 g
//
LOCUS       DAORPD010000412         1677 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_13775_length_1677_cov_3.146116, whole genome shotgun sequence.
ACCESSION   DAORPD010000412 DAORPD010000000
VERSION     DAORPD010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13775_length_1677_cov_3.146116"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(196..>1677)
                     /locus_tag="PK976_05440"
     CDS             complement(196..>1677)
                     /locus_tag="PK976_05440"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRC67362.1"
                     /translation="GSIEINNNPSWNDNNRYEAKIETGSFGYQSYGGKAAIKHKNLQT
                     KFYTRYQSADNDFEYINNMLAEPKKEKRLGASNHQWLVGEDFYLNADSLNAIEIHLFT
                     QSLNRDIPSPISTVQLPENEQMKLDDARIMASWRHQSKNQIRLGLKSAWTYNFMNYQN
                     KLAAINSTYTMHQGFLIINASKYWENNTHLESEIRFQSQMARTANYADNITRNISSLW
                     IKLNHHLGSFANLYAGMRKEIAESTSTPLLPVIGTELFPMATKNLAFYLAYAKNSRIP
                     ALNDLYWVPGGNPNLKPEISHHLESGLRFRQNLLNRISIGLQANAYSSRIKDWIAWMP
                     DSVMISIWEPINLKHVKASGIEILIDIGCTWSKTSLNSRLSYAFNITNENEDDPQGRL
                     QLPYTPQHIGNFSFYLVHRSFSFMYSMNYTGKRFTNYDESHFLKPYLLQDTGLDYIFS
                     IKKLEIKLTLKINNLFNIQWENIAYQPMPGRYFHLGFTFLLNP"
BASE COUNT          504 a          289 c          321 g          563 t
ORIGIN      
        1 atcataaaaa tcgatacttg catttccttg ctggaaaagc ccttcattaa tgataaaaaa
       61 gcctctacgt ccggaaaaat tattttcgga agtattttct ggattctcgg gctcattgct
      121 gcatgctgaa atgaacatga caagggaaaa acaaaaaaag taaaagaatt ttttgtaaag
      181 catggttttt aatttttaag ggtttaataa aaaagtaaat ccaagatgaa aatatcggcc
      241 tggcatgggt tgataggcaa tattctccca ttggatatta aataaattat taatttttaa
      301 tgttagttta atttcaagtt tttttatgga aaatatataa tctaagccag tatcttgaag
      361 cagatatggt ttcagaaaat ggctctcatc gtaattcgta aaacgtttac ctgtgtaatt
      421 catcgaatac ataaagctga aacttcgatg aacgagatag aaagaaaagt ttccaatatg
      481 ttgtggtgta tatggtaatt gaagtctccc ctgagggtca tcttcgtttt cgttggtaat
      541 attgaaagca taggaaagtc tgctattcaa cgaggttttg ctccatgtac agcctatgtc
      601 tattaaaatt tcgataccgc tggcttttac atgtttcaaa ttgatgggtt cccaaattga
      661 aatcattaca ctatcgggca tccatgctat ccaatctttt attcgggacg aataggcatt
      721 tgcctgtaaa ccgatagaaa ttcgattgag taaattttgt cggaaacgca aacccgattc
      781 gagatgatgg ctgatttcgg gttttaaatt aggattgcct cccggaaccc aatacaaatc
      841 attcaaggct ggaattctcg aatttttagc ataagcgagg taaaaggcaa gatttttcgt
      901 agccataggg aataattctg tcccaataac aggcagtaat ggcgtcgaag ttgattctgc
      961 gatttctttc ctcatacctg cataaagatt agcaaaagag ccaagatgat gatttagctt
     1021 tatccataga gaagagatat ttcgagtgat attatcggca tagtttgcag tacgagccat
     1081 ttgcgactga aaccggattt ctgattcgag gtgtgtgttg ttttcccaat atttggatgc
     1141 atttattatt aaaaatccct gatgcatagt ataagtagaa ttgattgcgg ctaatttgtt
     1201 ctggtaattc ataaaattat aagtccaggc cgatttcaag ccaagacgaa tttgattctt
     1261 gctctgatga cgccaggatg ccataattcg ggcatcatcc aatttcatct gttcattttc
     1321 aggtaattgg accgtcgaaa tgggcgaggg aatatctcta tttaatgatt gtgtaaaaag
     1381 gtgaatttca atggcattca aactatctgc atttaaatag aaatcttctc caaccagcca
     1441 ttgatgatta cttgccccaa gtcttttctc ttttttgggt tctgccagca tattgttaat
     1501 gtattcgaaa tcattatcag ccgactgata cctcgtataa aacttcgtct gaaggttttt
     1561 gtgctttatt gctgccttgc ccccatagct ttgataacca aaactccctg tttctatttt
     1621 agcttcataa cgattattgt cgttccagct gggattattg ttgatttcta tgcttcc
//
LOCUS       DAORPD010000413         1677 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_13780_length_1677_cov_1.760789, whole genome shotgun sequence.
ACCESSION   DAORPD010000413 DAORPD010000000
VERSION     DAORPD010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13780_length_1677_cov_1.760789"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="PK976_05445"
     CDS             complement(<1..284)
                     /locus_tag="PK976_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HRC67363.1"
                     /translation="MSSPNFVDYVKIYLRSGRGGAGSVHFRREKYIPKGGPDGGNGGR
                     GGHIILRANAQLWTLLPLKYTKHVFAGDGKPGAGNRRTGADGEDRIIEVPV"
     gene            complement(363..1532)
                     /locus_tag="PK976_05450"
     CDS             complement(363..1532)
                     /locus_tag="PK976_05450"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF001381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HRC67364.1"
                     /translation="MLNIALFGPPGAGKGTQSKKLIEKYNLTYVATGDLLRKEIAEGT
                     ALGIEAKNLIEKGRLVPDDMILQIIEKHINTNTNSNGILFDGFPRTYIQAYILEGLLM
                     RMHTSLTCLLSLEVPREELIERMLKRAEMENRSDDTLEVIEYRLLEYETKTTPVIEFY
                     KERNIYYPINGVGKVDEVFERLCQAIDNSLTKQWMNVVLLGPPGSGKGTQGQIIAKRH
                     ALGYIPTGTLLRREIKEGTDIGHRAKPYMEKGEIVPDEIAVKLIERELHLTSKVRGFV
                     FKGFPRTMVQAYILDGMLKKLNSSVTIAIELKVPTLECFKRLTMRAKTDKARTYDKDP
                     ELIIHRLEEYEQKTSLVKQYYKNQNKLIKVDGTGDVAEVSDRISEVIDKVARMGR"
     gene            complement(1547..>1677)
                     /locus_tag="PK976_05455"
     CDS             complement(1547..>1677)
                     /locus_tag="PK976_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HRC67365.1"
                     /translation="KNFKIDYLGFEIPNDFIVGYGLDYNGLGRNLPDIYQIVKTDS"
BASE COUNT          457 a          371 c          318 g          531 t
ORIGIN      
        1 acgggaactt ctatgattct gtcttcacca tcggctccag tacgacgatt gccggctcct
       61 ggcttgccat ctcccgcaaa aacatgtttt gtgtatttca aaggcagtaa agtccataat
      121 tgagcattag cacgcagaat gatatgacct cctctgcctc cattgccacc atcgggtcca
      181 cctttgggga tatatttttc acgtcgaaaa tgtacagaac ctgctcctcc tctacctgag
      241 cgaagataaa tctttacata atcgacaaaa ttaggcgatg acatgtcaaa ttcttaacca
      301 gatgcaaagt taatcgaaag ctaatgaacg ctcaagccct ctactcatta tgtatcatag
      361 ccttacctgc ccattcgggc tactttgtca atcacctccg aaatacggtc ggagacttcg
      421 gctacatcgc ctgttccatc cacctttatc aatttattct gattcttata atattgtttg
      481 accaagctgg ttttctgctc atattcttca aggcggtgaa taattagttc gggatcctta
      541 tcgtaagtac gagctttatc agtttttgct cgcatggtaa gacgtttgaa acattcgagt
      601 gtaggaactt tcaattcaat ggcaatagta acacttgagt taagtttttt aagcataccg
      661 tcgagaatat aagcttgcac catggttcgc gggaaacctt taaaaacgaa gcctctaact
      721 tttgacgtga ggtgaagctc gcgttcgatg agttttacag caatttcgtc gggaacgatt
      781 tctccttttt ccatataagg ttttgctctg tgtccaatat cagtcccttc tttaatttct
      841 cttctgagca aggtacctgt ggggatatat cccaaggcat gacgtttggc aataatttgg
      901 ccctgagtac ctttacctga tcccggaggg ccaagtaata ccacattcat ccattgtttg
      961 gtaagggaat tatctatggc ctgacacaac ctttcaaata cctcatcaac cttaccaact
     1021 ccattgatgg gataatagat atttcgttcc ttataaaact cgattacggg tgtggttttg
     1081 gtttcgtatt cgagcaagcg atattctatg acctcgagag tgtcgtcgct acgattctcc
     1141 atctcggctc gtttgagcat tctttcaatt aattcctctc gcggaacctc aagactcaaa
     1201 aggcaagtga ggctagtatg catacgcatg agtaaacctt cgagaatata agcctgtatg
     1261 taggttcttg gaaaaccatc gaacaaaatg ccattcgaat tggtattggt gttgatgtgt
     1321 ttttcgatga tctgaagaat catatcatca ggcactaaac gacctttttc gataagattc
     1381 ttagcttcta ttcctaaagc tgtgccttcg gcaatctcct tacgaagcaa atcgccagta
     1441 gcaacataag ttaaattgta tttttcgatt aattttttcg attgtgttcc tttccctgca
     1501 cccggagggc caaataatgc aatgttgagc atgaagttga attaaattaa gaatcggttt
     1561 ttacaatttg gtaaatatcg ggcaaatttc gccctaatcc attatagtcg agaccataac
     1621 ccacaataaa gtcattaggt atttcgaatc ccagataatc gattttgaaa ttcttac
//
LOCUS       DAORPD010000414         1676 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_13793_length_1676_cov_2.412708, whole genome shotgun sequence.
ACCESSION   DAORPD010000414 DAORPD010000000
VERSION     DAORPD010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13793_length_1676_cov_2.412708"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1676)
                     /locus_tag="PK976_05460"
     CDS             complement(<1..>1676)
                     /locus_tag="PK976_05460"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HRC67366.1"
                     /translation="NRRAIISDLSPAAAFISYNYNTPINTNKFELELRGIVEQVKKEC
                     EWLYETRHVDGRVGRINFTVWSDVFVCPYCKNDVIFWETGVDHENGKVLDAFQCQNCK
                     AQITKTEANRAKFTVQDPYIGSLVERSRQVPVMINYSLGKTRYEKKPDSFDLALLERI
                     NNSEIPFWVPIFRLPEGFNTEQPKNSHGATHVHHFYTKRNLWVLSCLFDKLRRTNSRY
                     LILGLSITSSLVSLLTRYNMGHRGNGQVSGTLYIPSLVAETSVIKVVENKIRDIANMS
                     ELSKDNHAIISTQSTTSLKNIPDNSIDYVFTDPPFGGNLMYSELNFLWEAWLKVFTNN
                     TDEAIVNNVQKKNLENYRELMESCFKEMYRILKPGRWITVEFHNSKASVWNAIQDSLS
                     HAGFVVAQVTVLDKQQGSFKQVTSAGAVKNDLVINAYKAREGFAKRLVSQAGQGLEVD
                     FIREHLGQLPLSANIERSKEMLYSKYLAYYVQHGYQVMYNGEQFYHALPQWGFEERDS
                     YWFADEAQANEYEKRKAKTFGKGGIQAQAVLFVSDERSARQWIWNFLDQP"
BASE COUNT          493 a          343 c          301 g          539 t
ORIGIN      
        1 gggctggtcc aggaaattcc agatccactg acgagcagac cgttcatcac ttacaaataa
       61 gactgcctga gcttggatac cacccttgcc aaaggttttt gctttgcgct tttcatattc
      121 gttggcttgt gcttcatctg caaaccaata actgtcacgt tcttcaaagc cccattgtgg
      181 taatgcatga tagaactgct caccgttata catgacttga taaccgtgtt gaacataata
      241 tgctaaatat ttcgaataaa gcatctcttt gctgcgttcg atattggcgg atagaggaag
      301 ttggcctaaa tgttcacgaa taaaatctac ctcaagacct tgtccggctt gcgaaacaag
      361 tctcttggca aatccttctc gggctttgta tgcatttatg acaagatcat tttttactgc
      421 accagcactg gttacttgtt tgaacgatcc ttgttgtttg tcgaggacag tcacctgggc
      481 gaccacaaaa ccagcatgtg aaagagaatc ttgaattgca ttccaaacac tagctttgga
      541 gttgtgaaat tctacagtta tccaccttcc aggtttcaag attctataca tttctttaaa
      601 acatgattcc attaattcgc gataattttc aagatttttc ttttgaacat tgtttacaat
      661 ggcttcatca gtgttattag tgaagacttt cagccaagct tcccataaga agttaagctc
      721 agaatacatt aggttccctc cgaatggggg atcagtaaaa acataatcaa tcgaattatc
      781 gggaatattt ttcagagacg tggtagattg cgttgaaatt atggcgtgat tatccttact
      841 taattcagac atatttgcaa tatcacgaat tttattttcc acaaccttta ttacactggt
      901 ttccgccacc aatgatggga tatataatgt tcccgatact tgtccattcc cacgatgtcc
      961 catattgtat cgggttagta aggatacaag gctagaggtt atagataaac cgaggatcaa
     1021 ataacgagaa ttggttcgtc gcaatttgtc gaaaaggcat gatagaaccc ataaattgcg
     1081 ctttgtataa aaatgatgca cgtgtgttgc gccatgagaa ttttttggct gttcagtatt
     1141 aaatccttct ggtaaccgaa atatgggcac ccaaaaagga atttcggaat tatttattct
     1201 ttccaataat gccaaatcaa atgaatctgg ttttttctca tatcgagttt ttcctaaact
     1261 gtaatttatc attacaggaa cctgacgaga acgctcgaca agtgatccta tataaggatc
     1321 ttgaacggta aatttggctc gattagcctc agtttttgtt atctgtgctt tgcaattctg
     1381 acattgaaaa gcatctaaaa ctttcccatt ttcatgatct actccagttt cccaaaaaat
     1441 gacatcattt ttacaatacg gacatacaaa cacatcactc caaaccgtaa aattgattct
     1501 gcctacccta ccgtcaacat ggcgcgtttc atacagccat tcacactctt tttttacctg
     1561 ttcgacaata ccccttaatt ccaattcaaa tttattagta tttattggag tattgtagtt
     1621 gtaagaaata aaggctgctg caggtgataa atcagaaata atagcccgtc ggttga
//
LOCUS       DAORPD010000415         1676 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_13795_length_1676_cov_2.257866, whole genome shotgun sequence.
ACCESSION   DAORPD010000415 DAORPD010000000
VERSION     DAORPD010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13795_length_1676_cov_2.257866"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            120..452
                     /locus_tag="PK976_05465"
     CDS             120..452
                     /locus_tag="PK976_05465"
                     /inference="COORDINATES: protein motif:HMM:NF039670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6150 family protein"
                     /protein_id="HRC67367.1"
                     /translation="MAFLSFVIIQAFSVQAQKVFKVDYPSQADLKVFIVSYESQADLL
                     VYFVKYASQTNRDGLFFPVKYASQADFKLYFTDYESQADLKIFIVDYESKAGWRNKSK
                     QYLLKFKK"
     gene            462..1511
                     /gene="mtnA"
                     /locus_tag="PK976_05470"
     CDS             462..1511
                     /gene="mtnA"
                     /locus_tag="PK976_05470"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HRC67368.1"
                     /translation="MKVNGKNYKTIWMEGKKVKLIDQNLLPFRFEIIELENWEGICKA
                     IKNMNIRGAGAIGVAAGYAMALAFVQSPGKGGEDYLWKSRKEIEQTRPTARDLFYATE
                     RVMQAGIAYGIEAAIEESEKLAIENEEEAKAIAIYGESLIPDSCNILTHCNAGWLGFV
                     DWGSALAPIYLAHRKGKKIFVYVDETRPRLQGSRLTAWELQNEGIAHVIVADNAGAWL
                     LSNNRVDLFITGADRIALNGDTANKIGTFEKAIVAKNFGVPFYVAAPLSTFDPACPDG
                     SCIPIEHRPLEEITEMEGLDEKHHFIKIRISSPGSEALNPAFDITPSSYIHSLITSKG
                     IIQPNNQAIRQLLFG"
BASE COUNT          548 a          270 c          355 g          503 t
ORIGIN      
        1 atgaatgggt aagaagccga aaattattcc ttaattttga aatgaactac aaaattagtc
       61 attcaaccca acttaaataa tttacataaa tgaggttcat gttgaaacca ataaaactta
      121 tggcgttttt atcttttgtt attattcagg cattttctgt acaagcgcaa aaggttttta
      181 aggtagatta cccttctcag gctgacttga aagtattcat cgtttcgtac gaatcgcagg
      241 ctgatttatt ggtttatttt gtgaaatatg catcacaaac caatagggat ggactgtttt
      301 tccccgtaaa gtatgcatca caagctgatt tcaagttata ttttaccgat tatgaatctc
      361 aagccgattt aaagattttt attgtagatt atgaatcaaa ggcaggatgg cgcaataaat
      421 cgaaacaata cctgttaaaa tttaaaaagt aagcaattgg aatgaaagta aacggtaaga
      481 attataaaac catctggatg gaaggtaaaa aggtgaaatt gatcgatcag aacctattgc
      541 cttttcgatt cgaaatcatt gaattagaga attgggaagg gatatgcaag gccattaaaa
      601 acatgaacat aagaggcgct ggggcaatag gagttgcggc tggttatgct atggcactcg
      661 cttttgttca atcccccggt aaaggaggag aagattattt gtggaaatcc cgcaaggaga
      721 ttgagcaaac tcgacctaca gcgagagatt tgttttatgc taccgagagg gtaatgcaag
      781 caggaattgc ttatggcatt gaggcagcaa tagaagaatc cgaaaagtta gctattgaaa
      841 atgaagaaga agcaaaagcg attgcgattt atggagaaag tttaataccc gattcatgta
      901 atatattgac gcattgtaat gcgggctggt taggttttgt agattggggg agtgcattgg
      961 caccaattta tttagcacat cgtaaaggga aaaagatttt cgtttacgtt gacgaaactc
     1021 gtccgcgttt acaaggttca aggcttacag catgggaatt gcaaaacgaa ggtatagcgc
     1081 atgttatcgt tgcagataat gcgggggctt ggcttttatc gaacaataga gtggatttgt
     1141 ttatcacggg ggccgataga attgccctga atggagatac ggccaataaa attgggacat
     1201 tcgaaaaggc cattgtggcg aaaaattttg gggtaccatt ttatgttgca gcaccattgt
     1261 ctactttcga tcctgcatgc ccagatggtt cctgcatccc tatcgaacac cgtcctcttg
     1321 aagaaataac cgagatggaa ggtttagatg agaagcatca ctttataaag ataagaattt
     1381 cttctcctgg ctctgaagct ttgaatccag ctttcgatat cactccatct tcctacatcc
     1441 acagcttgat tacttcgaaa ggaatcatac agcctaataa tcaggctatt aggcaacttt
     1501 tatttggtta accacaaaat gcaattttta taataaggaa ttactccaat tggatattaa
     1561 ttttagagga taacgagaat aatgtaaata taaattaatt ttcctcagtt ttcgatagag
     1621 aatttttttc aggttatttt ataaatttat ccaaaatccc gaaagcctat gaagac
//
LOCUS       DAORPD010000416         1675 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_13812_length_1675_cov_2.227160, whole genome shotgun sequence.
ACCESSION   DAORPD010000416 DAORPD010000000
VERSION     DAORPD010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13812_length_1675_cov_2.227160"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            255..836
                     /locus_tag="PK976_05475"
     CDS             255..836
                     /locus_tag="PK976_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67369.1"
                     /translation="MTIKRMTFIVAVLALFPGCSNQKKQERPAITFELSQLEEMATQY
                     VGKEIVLKGTIDHVCKHGGARMFLVGNTPGERIRIDAGENMKFDPAWEGNTAYITGIL
                     EELKIDSTYLDKWEQEVRAKKGREAASKIHTGEQGHEEAENNIENELQQIADYRTAIY
                     DSGKDYLSFYSVKCLKYQIVEEYDSNKKKDSVN"
     gene            919..1488
                     /locus_tag="PK976_05480"
     CDS             919..1488
                     /locus_tag="PK976_05480"
                     /inference="COORDINATES: protein motif:HMM:NF027675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HRC67370.1"
                     /translation="MKRFKWRKSNRVIHRDLGYFFVGMTIVYALSGIAFNHINDWNPS
                     FIITNKVVSLSSAPSLENINNQHIKEILRDCEESEEFKKFYISSDSILKIFIEHGTVT
                     VSTETREATIEKIRRRPLFHTFNYLHYNPGRVWTYFSDFYAVSLLVLATSGLFILPGK
                     NGIRWRGAILAGLGIVVIIVLLFLTDYSF"
BASE COUNT          572 a          276 c          307 g          520 t
ORIGIN      
        1 caaatttaca actgggggat gaaacttcgg ttttgtccct ccttcttttt atccattttt
       61 ttgtcggtca gtaatgataa attttgagtt ctaaatatca atatattgaa ttacaataat
      121 ttataaattt acatctttcg tcaaatcact ttattgttgt agcattctca aatatttcat
      181 aatattgcac gaaatcgtgc taagtaaaca ttactgaaat ttagttagat ttataaatca
      241 taaacacaaa cattatgact attaaaagga tgacatttat tgttgcggta cttgctttat
      301 tcccgggttg tagcaatcaa aagaagcaag aaagaccggc cataaccttt gaactttcac
      361 aattggaaga aatggccacc caatatgttg ggaaagaaat cgtattaaaa gggacaatcg
      421 atcatgtttg caagcatggg ggagccagaa tgtttttagt aggcaatact ccaggcgagc
      481 gcattcgcat cgatgccggt gaaaacatga aattcgaccc agcatgggag gggaatacgg
      541 cttatataac aggtattctc gaagaactca agattgatag cacatacctc gacaaatggg
      601 aacaagaggt aagggcaaag aagggacgag aagctgcttc aaaaattcac actggcgagc
      661 aaggccacga agaagctgaa aacaatatag agaatgaact tcagcaaatt gctgattacc
      721 gaactgccat ctatgatagt gggaaagatt atttatcgtt ctattcggta aaatgcctta
      781 aatatcaaat agtcgaagaa tacgacagca acaagaagaa agattctgtc aattaacttt
      841 cggacaatac aggattagga aatataagtg aatcattcta tcaaagtatt ttcataaatt
      901 cccctctttt ttattaaaat gaaaagattc aaatggagaa aatctaaccg ggttattcac
      961 agagatttag gatatttttt tgtgggaatg accattgttt atgcactttc ggggatagcc
     1021 ttcaaccata tcaatgattg gaatccgagt tttattataa ccaataaggt agtttctttg
     1081 tcttcagcac catcactcga aaatatcaat aatcaacaca tcaaggaaat tttaagagat
     1141 tgtgaagaaa gcgaagaatt caagaaattt tatatctcct ccgatagtat tctaaaaatc
     1201 tttatagagc atgggacagt aactgtgagt acagaaacta gggaagcaac aatcgaaaaa
     1261 atacgacgca gaccattgtt tcatactttt aattatctac attataatcc tggcagggta
     1321 tggacatatt tttccgactt ttatgcagtt tcattgttag tactagctac cagtggttta
     1381 ttcattttac cgggtaaaaa cggaataagg tggagagggg ccattcttgc agggttagga
     1441 attgtagtca tcatcgtatt attgtttcta acggattatt ccttttagca tagaataaat
     1501 tcagaactcc tgatcctata attgcatatc aatattttac aaaagtacta ccgtatttca
     1561 atttgtcgta ttttcggaca aaaattttta gatttgcaac ttcagttacc atttttccct
     1621 accgatactc gaatgattaa ttccaacgtt ggagtttttt cgaaagatga atttg
//
LOCUS       DAORPD010000417         1675 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_13815_length_1675_cov_2.148765, whole genome shotgun sequence.
ACCESSION   DAORPD010000417 DAORPD010000000
VERSION     DAORPD010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13815_length_1675_cov_2.148765"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1675
                     /locus_tag="PK976_05485"
     CDS             <1..>1675
                     /locus_tag="PK976_05485"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HRC67371.1"
                     /translation="NNNPHNPVINYRSIGENPQQVAYNASLLMQGMQQAGIMTTLKHF
                     PGHGDTETDSHNGLPEIKKQIQDLETNELIPFAYNLSLTDAVMVAHILIPSLDTVNTL
                     PASLSPVIVNHLLREQMGFRGLIITDALDMKGAGNYKQAGEKEWLAFIAGNDILLLPE
                     NIKAAIEKIKNAIDSTIIPYQFLEEKCKRVLAHKYKAGLHDFQPIKTTNLLQDLNQPY
                     YSTIIRDAFKGSLTLINEPVPVPINPVNYYKILYVPVFAENQAFGEQMKILFPHEKFI
                     LPSLADTVAIRQIFDSIKEYDLLITTIAPPSTFASKNYGLDKDFHSFINALPKNIPGI
                     LVLFGCPYIASYFGPLLNHQSIIITYQNNSITGELAANALYGDNAIRGHLPVSIDNNV
                     SSGTGIETEASQVLGFATPSEVDVDEGKLNKVDEIALEGIKKGAYSGCQILLAHHGKV
                     FYYKSFGYLDVTHRVPVKNSDLYDLASITKIAATTLVTMKLYDEHKIDNDTELKDLLP
                     NLKNRKLRNTKITDIMYHQAGLFPWIAYYKATLENGVPSKKYYRTQTEPG"
BASE COUNT          544 a          369 c          293 g          469 t
ORIGIN      
        1 gaacaataat ccccataacc ctgtcatcaa ttatcgttcg ataggagaaa accctcaaca
       61 agtggcttat aacgcctctc tattgatgca ggggatgcaa caggctggca ttatgacaac
      121 cctgaaacac tttccgggac atggagatac tgaaaccgat tcacacaatg gattacccga
      181 aattaaaaaa caaattcaag atcttgaaac caacgaattg attcctttcg cttataatct
      241 ttctcttacc gatgccgtta tggtcgctca cattttaata ccttcgctcg acacggtgaa
      301 tacattaccc gcaagccttt ctcctgtcat tgttaatcat ttgctgagag aacaaatggg
      361 ttttcgagga cttatcatca ccgatgcact cgacatgaaa ggagctggaa attataaaca
      421 agctggtgaa aaagaatggc ttgctttcat agctggcaac gatatcctcc tattgccaga
      481 aaacataaaa gcggctatcg agaaaattaa aaacgctatc gattcaacca taatccctta
      541 tcaatttctg gaagaaaaat gcaaacgggt attagctcat aagtataaag ctggccttca
      601 tgactttcaa ccaataaaaa ccacaaacct tttacaggat ttaaaccaac cttattattc
      661 cactattatc agggatgctt ttaaaggctc acttacgcta atcaatgagc ctgttccagt
      721 tccaataaat ccagtaaatt attataaaat tctatatgtt cccgtatttg ccgaaaatca
      781 ggcttttggc gaacaaatga agattttatt ccctcacgaa aaattcatac tgccttcatt
      841 ggcagatact gtggcgattc ggcaaatctt cgatagcatc aaggagtacg acttgctcat
      901 aactaccata gcaccccctt ctacgtttgc ttcgaaaaat tatgggctcg acaaagattt
      961 tcattctttc atcaacgcct tgcctaaaaa tatacccgga atactagttc tttttggttg
     1021 tccttacatt gccagttatt tcgggccttt attgaaccat caatcgatta tcatcactta
     1081 ccagaacaat tccattacag gtgaactggc agcaaatgca ttatatggag ataatgccat
     1141 tcgcggtcat ctcccggtct ctatcgataa taatgtatcc tcgggtactg gcatagaaac
     1201 tgaagcatca caggtgcttg gctttgcaac tccatcggaa gttgatgtag atgaaggcaa
     1261 acttaataaa gtggatgaga tagccctcga aggaattaaa aaaggcgctt acagtggatg
     1321 ccagattcta ttggcgcatc atgggaaagt gttctactac aaatcatttg gatatcttga
     1381 tgttactcat cgagtacctg taaaaaatag cgacttatac gatctggctt ccattacaaa
     1441 aattgcagcc actacgctgg ttaccatgaa actatatgat gaacataaaa tcgataatga
     1501 cacggagtta aaagacttat tacccaactt gaaaaaccgt aaactgcgaa atacaaaaat
     1561 caccgatatt atgtatcacc aagccggttt attcccatgg atcgcttatt ataaagccac
     1621 cttggaaaac ggggttccca gcaagaaata ttatcgtact caaactgaac ctggt
//
LOCUS       DAORPD010000418         1673 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_13836_length_1673_cov_2.901731, whole genome shotgun sequence.
ACCESSION   DAORPD010000418 DAORPD010000000
VERSION     DAORPD010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13836_length_1673_cov_2.901731"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..956
                     /locus_tag="PK976_05490"
     CDS             24..956
                     /locus_tag="PK976_05490"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRC67372.1"
                     /translation="MNRIFTIGETVMDIIFRDQHLETAMVGGAMLNTAVSLGRLHMPA
                     FLISEWGNDGVGEITQNFLTNNAVNTSFCKSYHGNTSLSVALIDTKGNASYTFYKNYP
                     EKRFQIDTPDFEPGDFVLFGSFFSIDPGIRTQMTNILKQARNSGALLIYDPNFRKNHL
                     ASLEQLLPFIEENIRYSHIIRGSHEDFHYIYRVDSCQKLFEKVHSLGCEVLIITHGSD
                     EILLFKECTKTRVAAAAVEAVSTIGAGDSFNAGLMYELYRRQISPNNLNMIPSCEWAE
                     IVSVASSFAANTCSHYENYISQEFAQHILSSQAI"
     gene            953..>1673
                     /gene="rhaD"
                     /locus_tag="PK976_05495"
     CDS             953..>1673
                     /gene="rhaD"
                     /locus_tag="PK976_05495"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: protein motif:HMM:NF002963.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HRC67373.1"
                     /translation="MKISIPELKPLLKEIAEVAYNVWAHGWAERNAGNISMNVTPHIR
                     TMDYISDKKQEQHRLPFLFPNLSEQILLMSATGSRMRELAEFPHKYMMLLRISSDGDS
                     YSIIPLANHSFHLQPSSELLAHLAIHQQLIEEDSLNNTLLHTHITEVIALNHFPNLNE
                     KNALNKVLLSMHPETILFLPEGAGFVPFLPPGSQELARKTLDEIKHHSVVIWEKHGVL
                     SYGTSPANACDQLELIAKAARI"
BASE COUNT          508 a          371 c          314 g          480 t
ORIGIN      
        1 gatagaatcg tacttttata aaaatgaaca gaatttttac aataggtgaa acagtaatgg
       61 atatcatctt tcgggaccaa cacttggaaa ccgccatggt gggaggtgcc atgctgaata
      121 ctgcagtaag tctcggtcgc ttgcacatgc cagcatttct catcagtgaa tggggaaacg
      181 atggagtggg agaaattaca caaaatttct tgacgaacaa cgctgtcaat acatcgtttt
      241 gcaaatctta tcatggaaat acttcacttt cggttgcttt aatagatacc aaaggcaatg
      301 cctcttatac tttttacaag aattatcccg aaaaacgatt ccagattgat actccagact
      361 tcgaacccgg cgattttgtg ctgtttggtt cattcttctc gatcgatccg gggattagaa
      421 ctcaaatgac aaatattctg aaacaagcca gaaacagtgg tgctttattg atttatgatc
      481 ccaatttccg taagaaccat ctcgcctcgc tcgaacaatt attacctttt attgaggaaa
      541 atatacgata cagccatatc atcagaggca gccatgaaga ttttcactac atctaccgag
      601 tggattcttg ccagaaactt ttcgaaaaag tccattcgct aggatgtgaa gttcttatta
      661 taacgcatgg cagtgatgag attctcttat tcaaagaatg cacaaaaact cgtgttgcag
      721 ccgctgccgt cgaagctgtg agtactattg gcgctggcga ttcattcaat gcgggcttga
      781 tgtatgaatt gtatcgccgt caaatatctc cgaataattt aaacatgatc ccatcgtgtg
      841 aatgggccga gatcgtgtcg gttgcttcat cttttgccgc taacacttgt agccattacg
      901 aaaattacat ttctcaagaa tttgcacaac acatcttatc ctctcaagca atatgaaaat
      961 ttcgattcca gaattaaaac ccctattaaa ggaaatagcg gaggtagcat ataacgtatg
     1021 ggcacatggt tgggccgaac gcaatgcggg taacatttcc atgaatgtca ctccacatat
     1081 tcgaaccatg gattatatca gcgataaaaa acaagaacaa catcgactcc ctttcttgtt
     1141 ccctaattta tcggaacaga tattattgat gagtgccaca ggcagccgca tgagagaact
     1201 ggctgagttt ccgcacaaat acatgatgct tttgcgtatt tcgtccgatg gcgacagcta
     1261 ctccatcatt cccttggcaa atcactcctt tcatttgcaa ccttcttccg agttacttgc
     1321 ccatctggcc attcatcaac aattaataga agaagattca ctaaataata cactattaca
     1381 tactcatatc accgaggtca ttgctttgaa tcattttccc aatctcaacg aaaaaaatgc
     1441 cctaaataaa gttcttttat ccatgcatcc cgaaactatt cttttcttac ccgaaggagc
     1501 cggattcgtg ccttttttgc ctcctggaag ccaggaactt gcacgtaaaa ctttagacga
     1561 aatcaaacat cattctgtag taatctggga aaaacacggc gtgctttctt atggaacgag
     1621 tccggcaaat gcctgcgacc aattagaact aattgccaaa gcagccagaa ttt
//
LOCUS       DAORPD010000419         1673 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_13839_length_1673_cov_2.335600, whole genome shotgun sequence.
ACCESSION   DAORPD010000419 DAORPD010000000
VERSION     DAORPD010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13839_length_1673_cov_2.335600"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1538
                     /locus_tag="PK976_05500"
     CDS             <1..1538
                     /locus_tag="PK976_05500"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HRC67374.1"
                     /translation="STGSFAYVRIKCRILMNARRKNTMKKFIFGWAGLVFILMLASCE
                     DFLELDPVSNGIAVGNAAGDSILYKTGAEVEAALAGAYADFKNEYYQLDYYVNGDAQS
                     DDAYAGADNPANFQIDDYKIDALNSNVARDWAYLYAHIGKTNAVINNVDKCPDASLSA
                     ARRKEIKAEALFIRAFQYFQLVQLWGDVPLQLTEVRTISAANLEEIYPLLYPPRTPLD
                     MVYQQIISDLATAARGVKVTAPHKGYATKGAVYTLLAKVYATIEPHDYNKVNLYCDSV
                     MALGYSLMPQYDQLWDNSQENCSESIFEINYNGGVSDGNWGAKMFRGLDWKKFNIPSN
                     DLVKIFEQEGDMIRFTSSIVWEDVSGRWTDPHWPQVHYPFINKWRNYAEGSDQNYIIY
                     RLADVMLLKAEALNEMGDVSGAASLVNQIRARVNLPETQANDQASMRLTIEKERRLEL
                     AFEGHRWYDLKRTHRAIEVINNVKGENDIPLGYQLTENRLLWPIPQAELDKNSKLTQN
                     PGY"
BASE COUNT          480 a          358 c          361 g          474 t
ORIGIN      
        1 tatccacagg ctcgttcgct tatgttagga ttaaatgtag aattttaatg aatgccagga
       61 ggaaaaatac tatgaaaaaa ttcatatttg gatgggcagg ccttgtattt attctaatgc
      121 tggcctcctg tgaagatttt cttgaactcg atccagtatc caatgggata gccgtaggaa
      181 acgctgcagg cgattctatc ttgtacaaaa ctggtgcgga ggttgaagct gccctcgctg
      241 gcgcttatgc cgatttcaag aatgaatatt atcagttgga ttactacgta aatggagatg
      301 ctcagtcgga tgatgcttat gcaggcgccg ataatcctgc aaattttcaa atcgacgatt
      361 acaaaatcga cgcactgaat tcgaatgtag ccagagactg ggcttacctc tatgcacata
      421 ttggaaaaac caatgctgtc atcaacaatg tcgataaatg tcctgatgct tcgttatcgg
      481 cagctcgcag aaaggagata aaggccgaag cattgtttat cagagctttc cagtattttc
      541 aactcgtaca gttgtgggga gatgtgcctc ttcaactcac cgaagtacgt actatcagtg
      601 cagccaacct cgaggaaatc tatcctttgc tgtatcctcc aagaacgcct cttgacatgg
      661 tttatcagca aattatttcc gatttggcaa cagctgccag aggagtaaaa gttactgctc
      721 ctcataaggg ttatgccaca aaaggtgcag tctatactct tctggccaag gtttatgcca
      781 ctatcgagcc acacgattat aacaaggtaa acctttactg cgacagtgta atggcgctcg
      841 gatattcact tatgcctcaa tatgaccaat tgtgggataa ttcgcaggaa aactgttccg
      901 aatctatctt cgaaatcaat tacaatgggg gcgtttcgga tggtaactgg ggagcaaaaa
      961 tgtttcgtgg cctcgactgg aaaaaattca atattcccag caacgatctc gtaaaaatct
     1021 tcgaacaaga aggcgacatg attcgtttta cttcatctat cgtttgggaa gacgtttcag
     1081 ggcggtggac cgatcctcac tggccacagg ttcattatcc cttcatcaat aaatggagaa
     1141 attatgccga gggaagcgac caaaattaca ttatttatcg tttggcagat gtaatgctgc
     1201 taaaggctga agccttgaac gagatgggtg atgtaagtgg agccgcaagt ctagtaaatc
     1261 agattcgtgc aagagttaat ttacccgaaa ctcaagcaaa tgatcaagct tccatgcgct
     1321 tgaccatcga aaaggaacgt cgtcttgaat tagccttcga aggtcataga tggtatgatc
     1381 tgaaacgtac gcatcgtgcc atagaggtca tcaataatgt aaaaggcgaa aatgatattc
     1441 cccttggtta tcagcttacc gagaaccgtt tactttggcc tattcctcaa gctgaacttg
     1501 ataagaactc aaaactcacc caaaatcctg gttattgatc ttacggggcg caagctttca
     1561 aagaaagctt gcgcctcttg ctttttcatc tttgcccagt ccttttatta tcatgaaaac
     1621 tctgatcccc gtttcctcga tgatttactc tcttctattg ctatcagcac ttg
//
LOCUS       DAORPD010000420         1670 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_13879_length_1670_cov_2.654489, whole genome shotgun sequence.
ACCESSION   DAORPD010000420 DAORPD010000000
VERSION     DAORPD010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13879_length_1670_cov_2.654489"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..383
                     /locus_tag="PK976_05505"
     CDS             54..383
                     /locus_tag="PK976_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019152175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRC67375.1"
                     /translation="MGNTLFSAGEIMQFTQKVEYSKEGIVSKVVIDKSSGTITLFAFD
                     QGQRLSEHSAPFDALVQVIEGEAEIIINGKSFMLKAGDSIIMPAGIAHAVNAPDRFKM
                     LLTMIER"
     gene            528..>1670
                     /gene="hcp"
                     /locus_tag="PK976_05510"
                     /gene_synonym="priS"
     CDS             528..>1670
                     /gene="hcp"
                     /locus_tag="PK976_05510"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HRC67376.1"
                     /translation="MFCFQCQEATKGVGCTVKGICGKTPQVANLQDLLLFVLKGIAIY
                     SSALRRAGEKNLPNRFLVEGMFVTITNANFDADAIRERITKGLQIRDRLKVRANELGL
                     HPEFSDHDAATWLAVEDKYEIIAKQVGVLSTEDPDIRSLHELLIYGLKGMSAYTEHAM
                     NLEFEDEALFSFLEEGLKATLNPNLSLDELTSLILECGRHGVLAMSLLDRANTTRYGN
                     PEITKVNIGVGKNPGILISGHDLKDIEELLAQTEGTGIDVYTHSEMLPAHYYPEFKKY
                     RHFVGNYGGSWWKQNEEFETFNGPILFTTNCIVPPRSTSTYSNRVYTTGAAGHQGFKH
                     IPDRIAGKPKDFGEIIEHAKNCKPPVEIEQGEITGGFAHEQVFALAD"
BASE COUNT          513 a          292 c          368 g          497 t
ORIGIN      
        1 aaagaaagaa agttgtatta taatcaatta aataacaatt aattataaca aaaatgggaa
       61 acacgttatt ttctgctggt gaaatcatgc aatttaccca aaaagtagag tattcaaaag
      121 aaggaatcgt gagtaaagtg gtgattgaca aatcatcggg aacgattacc ttgtttgcgt
      181 tcgaccaggg tcagcgcttg agtgagcatt cggcgccttt cgatgccctt gtgcaggtta
      241 tcgaagggga agctgagatc attatcaatg gaaaatcttt tatgctaaaa gctggcgatt
      301 ctattatcat gcccgctgga atcgcacatg cagtgaatgc acccgataga ttcaaaatgc
      361 ttttaaccat gattgagcgt tgattctttt gattgatctt atgtataaac gtttataaac
      421 ttaatcgttc aattatttta acttcaaact ttaatgctat gagttttatt cattatttac
      481 ggttagaaga aaccgaagga aaccatctat cgaattccag acctgagatg ttttgttttc
      541 aatgtcagga agctaccaaa ggggtaggat gtacagtaaa aggaatttgt ggcaaaacgc
      601 ctcaagttgc taatcttcaa gatcttttgt tatttgtatt gaagggcata gcgatttata
      661 gttctgcctt acgtagagcg ggagaaaaga accttcccaa tcgctttttg gtcgaaggaa
      721 tgtttgttac gattactaat gccaattttg atgctgatgc catacgtgag cgcattacaa
      781 aaggattgca aattcgtgac agactgaagg ttcgtgcaaa tgaattggga ttgcatcccg
      841 agttttccga tcatgatgca gccacatggt tggctgtaga ggataaatat gagattattg
      901 cgaaacaggt aggcgttttg agcaccgagg atccagatat tcgttcattg catgagctgt
      961 tgatatatgg tttaaaaggt atgtcggcct acactgaaca tgcaatgaat cttgaatttg
     1021 aagatgaagc cctgttttct tttcttgaag aaggattgaa agctacattg aatcccaatc
     1081 ttagtttaga tgaactcact tctcttattt tagaatgtgg aaggcatgga gttttggcca
     1141 tgtcgttgct cgatagggca aatactactc gttatgggaa tcccgaaata acgaaagtaa
     1201 acattggtgt tggaaaaaat ccaggtattc tcatcagcgg tcacgacctg aaagatatag
     1261 aagagttact tgcacagaca gaaggaacag gtatcgatgt ctatacacat agcgaaatgc
     1321 tgcctgcaca ttattatcca gaattcaaga aatataggca ttttgtgggc aactatggcg
     1381 gttcgtggtg gaaacagaat gaagaattcg aaactttcaa cggtcctatt ctgtttacca
     1441 caaattgcat agtaccgcca cgttccactt ctacttattc caatcgggtt tatactactg
     1501 gtgcagctgg ccatcagggc ttcaagcata ttcctgatcg tatagccggg aagcccaaag
     1561 atttcggtga gattatcgaa catgcaaaaa attgcaaacc tcccgttgaa atcgaacaag
     1621 gtgaaattac aggaggtttt gctcatgaac aggtttttgc acttgccgat
//
LOCUS       DAORPD010000421         1670 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_13885_length_1670_cov_2.245820, whole genome shotgun sequence.
ACCESSION   DAORPD010000421 DAORPD010000000
VERSION     DAORPD010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13885_length_1670_cov_2.245820"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="PK976_05515"
     CDS             complement(<1..231)
                     /locus_tag="PK976_05515"
                     /inference="COORDINATES: protein motif:HMM:NF020977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HRC67377.1"
                     /translation="MSLEILINNDIKQAMLAKDTRKLEALRAIKAALLLEKTGKDISG
                     GEIPAEVEVKTLQRLIKQRKEAANIYTEKGRQD"
     gene            complement(258..>1670)
                     /gene="ftsZ"
                     /locus_tag="PK976_05520"
     CDS             complement(258..>1670)
                     /gene="ftsZ"
                     /locus_tag="PK976_05520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HRC67378.1"
                     /translation="QCNTDAQILEASPIPTRIQLGKSGLGAGNRPDIGRKAAEESIEQ
                     IRQILENNTKMLFITAGMGGGTGTGAAPVIASVARELDILTVGIVTIPFSFEGRKRKL
                     QAEQGIEELKKYVDTLLVINNDRLRDFYGNLKLREAFAHADDVLTRAAKGIAEIITVK
                     AYINVDFEDVKTVMQNSGTAIMGSSVASGENRALKAAEEALASPLLNDNDINGARNML
                     LYISSSKDNEITMDEVTEITAYIQNNAGNDVDVIWGVGYDENLGDAIGITIIATAFEA
                     PKTKQDPKAQVAKEKNIFNLNSEEPIPFVSNPSTEENNESIRLVSQSVPEKTPTANET
                     ISQEPSQLKKPAQRNIYPLFPEEENPTAHSPYTIPESSNEPEIAKPFTGTEPSYSISE
                     KVEAKNTERVNVLRGFSYPIKSLDQVEKLENEPAYKRRNKNLEPPPLSSESLAPKSAI
                     SDQPGGPEIRSNSYLFDNPD"
BASE COUNT          353 a          339 c          404 g          574 t
ORIGIN      
        1 gtcctgacgg cccttttctg tataaatatt tgctgcttct ttcctttgtt tgatgagtct
       61 ctggagtgtt ttcacctcta cttcggcagg tatttcgcct ccggaaatat ctttcccagt
      121 tttttctaaa agaagagcag ccttaatggc gcgaagcgct tcgagcttgc gtgtatcttt
      181 tgcaagcatg gcttgcttta tatcattatt gattagaatt tcgagcgaca ttttattgca
      241 aattcaaggg ttggaattca atcgggatta tcgaacaaat aagaattgga gcgtatttcc
      301 gggcctccgg gttggtcgga gatagcggat tttggtgcca gcgattctga tgaaagagga
      361 ggtggttcca gattcttgtt acgccgctta tatgcgggtt cattctccag tttttccacc
      421 tgatcgagtg atttgatagg atagctgaaa cccctcaata cattcactct ttctgtgttc
      481 ttagcttcga ctttttcaga aatcgagtag gagggttcgg tgcctgtgaa aggtttggct
      541 atttcgggtt catttgagga ttcgggtata gtataaggtg aatgagcagt ggggttttct
      601 tcctcgggga aaagaggata gatgtttcgt tgagctggct ttttaagttg tgatggttct
      661 tgtgaaattg tttcattcgc agtgggagtt ttttcgggta ccgattggct aaccaatctt
      721 atggattcat tgttttcctc tgttgaagga tttgaaacaa aaggaattgg ttcttcgctg
      781 ttgaggttga aaatattctt ttcctttgct acctgagctt tgggatcttg ttttgttttt
      841 ggagcctcga aagcagtagc aataatggta attccgatgg catcgcccag attttcgtcg
      901 taacctacac cccagatgac atctacgtcg ttgcctgcat tattctggat ataggcagta
      961 atttcggtca cctcgtccat agtaatttcg ttatcttttg acgaggagat gtaaagcagc
     1021 atattgcgtg ctccattgat gtcattgtca ttgagtaaag gtgaggcgag ggcttcttct
     1081 gcggctttga gggctctatt ttcacctgag gctacgctcg atcccatgat ggcggtaccg
     1141 ctgttttgca taactgtctt tacgtcctcg aagtcaacat tgatataggc tttgacggtt
     1201 atgatttcgg caattccctt agctgcacga gtcaagacat catcggcatg ggcaaaggcc
     1261 tctctgagtt ttagattgcc ataaaaatcc cttaacctgt cattgttgat cactaaaagt
     1321 gtgtcgacgt attttttcaa ttcttctatg ccttgttcgg cttgcagttt acgtttacgc
     1381 ccttcgaagc tgaagggtat ggttactatg cccacggtaa gtatatcgag ctcgcgagct
     1441 accgatgcaa ttacgggagc tgctcctgtt cctgttcctc ctcccatccc ggcagtaata
     1501 aacagcattt tggtgttgtt ttccaagatt tgccggattt gttcgatgga ttcttcggca
     1561 gcttttctgc caatatcggg tctgttgcca gccccaaggc ctgatttccc gagttgaatc
     1621 cttgtgggta ttggactggc ttccaatatt tgtgcatccg tgttgcattg
//
LOCUS       DAORPD010000422         1668 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_13910_length_1668_cov_2.488531, whole genome shotgun sequence.
ACCESSION   DAORPD010000422 DAORPD010000000
VERSION     DAORPD010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13910_length_1668_cov_2.488531"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="PK976_05525"
     CDS             complement(<1..404)
                     /locus_tag="PK976_05525"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HRC67379.1"
                     /translation="MANKLENEAMKFFRFVLLIGSLLLIFSCTHRNENNLKGDLVKNP
                     VSLKKSDSTQSPVMKFETLEHDFGRVFAGEKVSYSFKFYNKGKSDLLITNVTASCGCT
                     VPEWSKTPVAPGEEGHITVTFNTAGRSGFQQK"
     gene            484..999
                     /locus_tag="PK976_05530"
     CDS             484..999
                     /locus_tag="PK976_05530"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HRC67380.1"
                     /translation="MLIYLVGYMASGKSYTGSRLASKLGIRFIDLDHSIEEKYRISIK
                     NFFLKYGEDQFRIIENQMLHRTFQLADAVIATGGGTACYFDNMEQMNRHGLTIYLKQD
                     AEEVFQRLKRSKKPRPMLSASPDPELKEEILTQLRERVPYYEKAHIIFDIHKMNIHQL
                     ANEVLQYKNQF"
     gene            complement(990..>1668)
                     /gene="rlmD"
                     /locus_tag="PK976_05535"
     CDS             complement(990..>1668)
                     /gene="rlmD"
                     /locus_tag="PK976_05535"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HRC67381.1"
                     /translation="KETFTEIKSLYYLINTKANDSFSELEPILYAGQEVIIEEMDDLK
                     FRISPLSFFQTNILQTKCLYKVAMDFVELKAHEIVYDLYTGTGTIACFVAKHCQKVIG
                     IEYVERAIEDARMNARMNSIENVEFLSGDVLQIMNQDFIAKYGFPDVIITDPPRSGMH
                     PQVVKRMLDILPSRIVYISCNPATQARDVSLLSEKYHVRKVQPIDMFPHTSHVENVML
                     LERKDQN"
BASE COUNT          513 a          349 c          286 g          520 t
ORIGIN      
        1 tgcttttgct ggaatccact tcggccggct gtattaaagg taacagtaat atggccttcc
       61 tctccgggcg caacaggcgt tttggaccac tcaggcacag tgcatccgca acttgcagtc
      121 acattcgtaa ttaaaagatc agatttgccc ttgttataaa acttaaaact atacgaaact
      181 ttctctcccg caaatacacg gccaaaatcg tgttccaagg tttcgaattt catcactggc
      241 gattgagtag aatcgctttt cttcaaactt acaggattct tcaccagatc accttttaga
      301 ttattttcgt ttctatgggt acaggaaaaa atcaacagca aacttcctat caataagacg
      361 aaacggaaaa acttcatagc ttcgttttct aatttattgg ccacaaaatt acaacttctc
      421 tcgtaatttt ttcaatcatc aaatgcatcg acttattttt gcacctcctt aattcaccat
      481 aacatgctta tctatctggt aggttatatg gcaagcggta agtcatacac cggctctcga
      541 cttgcaagca aattgggcat tcggtttatc gatctcgatc actccattga agaaaaatac
      601 agaatcagta taaagaactt cttcctcaaa tatggagaag accaatttcg aatcatcgaa
      661 aatcaaatgc ttcatcgaac ctttcaactt gccgatgctg taattgctac cggaggtgga
      721 acggcttgct atttcgataa catggaacaa atgaatcggc acggacttac aatatattta
      781 aaacaagatg ccgaggaagt ttttcaacgg ttgaaacgat cgaagaaacc ccgtcctatg
      841 ctttcggctt cccccgatcc cgagttgaag gaagaaatat taacccagct aagagaaaga
      901 gttccttatt atgaaaaagc ccatattatt ttcgatattc ataaaatgaa cattcatcaa
      961 ctggctaatg aagttttaca atataaaaat cagttttgat cttttctttc gagaagcatt
     1021 acattctcga catgtgaagt gtgcggaaac atatctattg gctgaacctt tctaacatga
     1081 tatttttccg ataacaaact tacatctctt gcctgtgtgg ctggattgca actgatatat
     1141 acaatacgtg aaggcaaaat atcgagcatt cgttttacta cttgcggatg catcccactg
     1201 cgcggtggat ccgtaataat cacatcagga aagccatatt tcgcgataaa atcctgattc
     1261 attatctgaa gcacatcgcc tgatagaaat tctacattct ctatactatt catacgggca
     1321 ttcatgcgag catcttcaat ggctctttct acatattcta ttccaatcac tttttggcag
     1381 tgttttgcta cgaaacaagc aatcgtgcca gtgcctgtat aaagatcata aacaatttca
     1441 tgagctttta attcgacaaa atccattgca actttgtaaa gacattttgt ttgtagtata
     1501 tttgtctgaa aaaatgacag aggactgatt ctgaatttca aatcgtccat ttcttcgata
     1561 ataacttcct gacctgcata taaaatcggc tcgagttctg aaaaagaatc atttgccttt
     1621 gtattgatga ggtagtagag cgatttgatt tcggtaaaag tttctttt
//
LOCUS       DAORPD010000423         1667 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_13932_length_1667_cov_2.171836, whole genome shotgun sequence.
ACCESSION   DAORPD010000423 DAORPD010000000
VERSION     DAORPD010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13932_length_1667_cov_2.171836"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..929
                     /locus_tag="PK976_05540"
     CDS             <1..929
                     /locus_tag="PK976_05540"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HRC67382.1"
                     /translation="QLVAGPIERYDRLSPQLKSPHHPSYENFSNGLRLILFGLFIKMV
                     IADNLAPYVEEIYSQPSAYNTYHLLAGLGFYSFQIYSDFYGYSTIALGSALLLGVNLM
                     DNFKTPYLSANIREFWRRWHISLSTWFRDYVYIPLGGNRVKWSRWALNIFIVYTLSGL
                     WHGANWTFLIWGTIFGLMFIIERFCYERFGITSTQKWGIRRIFGVIITFTIVTLAWIF
                     FRSSSLSNALTMFEALWNNTALADSWRPDPFIMFWLSLFILLDILLFNNRFDKWCSKK
                     PLWLRWTIYTLLIFGITAFSGIDNQPFIYFQF"
     gene            929..>1667
                     /locus_tag="PK976_05545"
     CDS             929..>1667
                     /locus_tag="PK976_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67383.1"
                     /translation="MNSLATKILPRLFVLAIIAFLLNWIYSVAFYPSDLKKIDLIDSL
                     QKVIPRSDVIYFGESSNFSFVFEDDDQRRISDFISDYYPGLQFGTVNKGALHAGIYLK
                     LLKQIPTEAPVNTIILSMNLRSFSADWIYSKLESLLQRDMVLISDGPPLWNRFRLGLK
                     AYDNKTQKQRSMQVKNALKKDLLFLEPPCDFPNAVAWIENINNSPTSPWIEDSLAKSL
                     AISFIINYGFRIDTVNNPRIRDFDKIIE"
BASE COUNT          458 a          376 c          309 g          524 t
ORIGIN      
        1 ctcaactggt ggccggacca attgaacgct acgacaggct ttcacctcaa ttaaaaagcc
       61 cacatcatcc ttcttacgaa aacttttcta acggcttaag acttatcctt tttggattgt
      121 tcattaaaat ggtcattgcc gataatctgg ctccttatgt agaagaaatt tacagtcaac
      181 cctcggctta caatacatat caccttttgg caggacttgg attttattca tttcagattt
      241 attctgattt ttatggatat tcgaccatag cccttggctc tgctttactt ctgggagtca
      301 atttaatgga taatttcaag accccctatt tatcggcaaa tattcgggaa ttctggagac
      361 gctggcatat cagcctaagc acatggttcc gagattatgt atatattcct cttggaggca
      421 atcgtgtaaa atggagcaga tgggcactta atatctttat cgtttacaca ctaagcggat
      481 tatggcacgg agctaactgg acattcttga tctgggggac tatcttcgga ttgatgttca
      541 tcatcgaaag attttgctac gaacgctttg gaattactag tacgcaaaaa tggggcatca
      601 gaagaatttt tggtgtaatc ataactttta ccatagttac gcttgcctgg atttttttcc
      661 gcagcagctc tttatcgaat gccctgacca tgttcgaggc attatggaac aacaccgcat
      721 tagccgattc ttggcgaccc gatcctttta ttatgttctg gcttagcctg tttattcttc
      781 tcgatatact cctcttcaat aaccgttttg acaaatggtg ttcaaaaaaa ccattatggt
      841 tgcgatggac tatctatacc ttgctcattt tcggcattac agcctttagt ggaatcgata
      901 atcaaccctt tatatatttc cagttctgat gaattcgctt gccacgaaaa tattgccccg
      961 cctgttcgtt ttagccatta ttgcctttct attgaactgg atctattctg ttgcgttcta
     1021 tcccagcgac ctgaaaaaaa tcgacctcat cgattcttta cagaaagtta tacctcgatc
     1081 agatgtcatt tatttcggag aatcctccaa ttttagtttt gtttttgaag acgacgacca
     1141 acgccgcata agtgatttta tatccgatta ttatcccgga ctgcaatttg gcacagtcaa
     1201 caaaggagcc ttgcacgccg gtatttactt gaagctgctg aaacaaattc ccacggaggc
     1261 tcccgtaaac acaatcattc tgagcatgaa cctccgttcg ttttcggccg attggatcta
     1321 ttcgaaactc gaaagcctgt tgcagcgcga tatggtattg atcagcgatg ggcctcccct
     1381 ttggaatcgc ttccgactgg gactcaaagc ctacgataat aaaacccaga aacaacgtag
     1441 catgcaggtt aaaaatgcct taaaaaaaga cctactcttc ctcgagcctc catgcgattt
     1501 tcccaatgca gtcgcatgga tcgaaaacat caataattcg ccaacttctc catggatcga
     1561 ggatagttta gcaaaatcac tggcaatttc attcatcatt aattatggtt ttcgaatcga
     1621 tacagtcaat aatccaagaa tacgcgattt cgataaaatc atagaat
//
LOCUS       DAORPD010000424         1665 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_13959_length_1665_cov_2.366460, whole genome shotgun sequence.
ACCESSION   DAORPD010000424 DAORPD010000000
VERSION     DAORPD010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13959_length_1665_cov_2.366460"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(265..1500)
                     /locus_tag="PK976_05550"
     CDS             complement(265..1500)
                     /locus_tag="PK976_05550"
                     /inference="COORDINATES: protein motif:HMM:NF014135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HRC67384.1"
                     /translation="MGPFYIALIIFILTYTGIIFTRLPGVNIDRPSAAFFGAIAMVLF
                     GILSFDEAIAAIDFNTIGLLLGMMIIIATLQLDGFFAFVASQTIRIARTPFKLLITIT
                     LFTGITSAFLVNDAVVLFITPLIINLCKNGQLNPIPFLIAEILAANTGSLMTMTGNPQ
                     NMLIGINSGIPYTEFLLRLLPITLSGLFIIILVIRLLYNKTFSQKHEFTFDKNVFQYN
                     FRSMQWSVIILAGVIVMFFFQHLLHLSIPLISLTGAALILIFGKVKPSIVIKQIDWVL
                     LLFFASLFIVVHGVEKVGLFDRFITGNLLVENSRSLVFIHIISLVFSQLISNVPLTVV
                     MLPMMKTAGSEILWLGLASASTLAGNATIIGAMANLIVIESAQNKGVKISFMDFFKPG
                     IIVSLSTLLISFLVLIAQL"
BASE COUNT          582 a          298 c          325 g          460 t
ORIGIN      
        1 gaacctgaaa attgcctttc caccattttt tctctgaaat attcatgtag ggattataca
       61 aactgtcccc tttaaatggg tttggaggag aaaaagtgta aaccgggcaa cttgtataat
      121 accataacgt aatcaacaaa atcaggccaa tgaaaaataa taccagatat gcaatcattc
      181 tacccgcttt ttttatatat ctaaaaaacc tttgaggtct tctttttacc ataatctaca
      241 aaattaagga aaaagtgaat cctgttaaag ttgagctatc aaaaccagaa atgatatcaa
      301 taaggtggaa aggctaacta taatgccggg tttgaagaaa tccatgaatg atatttttac
      361 gcctttgttt tgggctgatt ctattacgat aaggttagcc attgcaccaa tgatagtagc
      421 atttccagcc aatgtagaag ctgaagccag cccaagccat aaaatttcgc taccggcagt
      481 tttcatcatg ggcagcatga ctaccgtcaa aggcacattg ctaattagct gtgaaaaaac
      541 taaactgata atatggataa agacgaggct tctactgttt tctacaagta aattccccgt
      601 aataaaacga tcaaaaaggc ctaccttttc tactccatga accacaatga acaaggaagc
      661 aaaaaaaagc agaagaaccc agtcgatttg cttgatcact atggatggct tgacttttcc
      721 aaaaatcaat ataagtgcag ccccagtcaa tgatatcaaa ggaatactga gatgaagcag
      781 atgttgaaag aaaaacatca cgataactcc agccagaata ataactgacc attgcatcga
      841 gcgaaaatta tattgaaaga catttttatc gaaagtgaac tcatgtttct gtgaaaaggt
      901 tttattgtat agtaacctga taaccaatat aatgataaaa aggcctgaaa gtgtaatagg
      961 cagaaggcga agcaggaatt cggtataagg aatcccagag tttatgccga tgagcatatt
     1021 ttgcgggttg cctgtcatag tcatcaaact tcctgtatta gccgccaaaa tttcggcaat
     1081 cagaaaggga atagggttga gctggccatt cttgcacaaa ttgataatta atggagtgat
     1141 aaaaagaact acggcgtcgt ttactaaaaa agcacttgtt atacccgtaa acagcgtgat
     1201 ggtgatgagt aatttaaagg gagttcgggc gattcggatt gtctgggagg cgacgaaagc
     1261 aaaaaaacca tctaattgca gagtagcaat gatgatcatc atacccagca gtaatccgat
     1321 ggtattgaaa tcgatggctg ctatcgcttc atcaaagctc agaataccaa agagcaccat
     1381 ggctattgcg ccaaagaagg ctgctgaggg tctatcgata tttactcctg gcaaacgggt
     1441 aaagatgatg cccgtatatg taagaataaa aataatgagt gcgatataga aaggtcccat
     1501 ccgtcagatt tccatagttc cgtaaatttg cacatccttg tttaatccca aaccctccaa
     1561 aattaggtaa tatattttga aaaagagtaa aataattgga tagatcgttt gtctattcga
     1621 tttttttgtt gtttctttgt attacctaaa taagtaatat atgga
//
LOCUS       DAORPD010000425         1663 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_13997_length_1663_cov_2.211443, whole genome shotgun sequence.
ACCESSION   DAORPD010000425 DAORPD010000000
VERSION     DAORPD010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13997_length_1663_cov_2.211443"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1452
                     /locus_tag="PK976_05555"
     CDS             <1..1452
                     /locus_tag="PK976_05555"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRC67385.1"
                     /translation="NPTIAGSHTTNGSGLGTFTSQITDLEPITTYYVRAYATNTYGTA
                     YGNEETFTTLTAPYPPIIDFVASSTNINVGNTVNFTDLSTNGPTSWSWSFVGGIPPSS
                     NQQNPSGILYSYPGEYTVCLTASNQYGSNTLCKDAYINVHAPIEAKIVITEIMYNPPE
                     TGTDSLEFIELFNNGASAIDLEGFYFSEGVTYTFPSYLLAPGAYAVVAVNSQAMQNTF
                     GISTLQWSSGALNNSGELIRLKDKYGYTVDSVYYDDALPWDTLADGWGPSLTLCNPDL
                     DNSLAENWTHSMEFVTVNANGDSIWATPGGPCIIPVLPVADFVGNPLSLMTGESVQFT
                     DLSTGPNITNWEWIFDGGTPATFSGQNPPPIYYYESGLYDVTLKVTNEFGTSTAFKPE
                     YITVLIDAIGDDISLIPISIQPNPSTGKVNIKHNQGKLLLEVFNLVGEKVYSQELNPL
                     TQDVDLTPLQKGIYFMRFTNDNGVKLTHKLILK"
BASE COUNT          478 a          362 c          304 g          519 t
ORIGIN      
        1 aaccctacta ttgccggttc ccataccacc aatggttcgg gattaggtac tttcacaagc
       61 cagattacag atttggagcc aattacaact tattatgtaa gagcttatgc taccaatacc
      121 tacggaacgg cttatgggaa tgaagagaca tttacaaccc tcaccgctcc ttatcctcct
      181 attattgatt ttgtggcttc cagcactaac atcaatgtgg ggaatacggt taattttacc
      241 gatttaagca caaatggacc tacttcgtgg tcatggtctt tcgtgggagg cattccaccc
      301 tcgtctaacc agcaaaatcc ttcaggaatt ctttattctt atcctggaga atataccgtt
      361 tgtttgaccg cttcgaatca atatggaagc aatactctgt gcaaggatgc ctatattaat
      421 gtacatgccc ctattgaggc aaaaattgtg ataaccgaaa tcatgtataa tcctcccgaa
      481 acaggtaccg atagcctcga atttatcgaa ctcttcaata atggagccag tgccatagat
      541 ctcgaaggat tctatttctc ggaaggagta acttatactt tcccttctta cttgcttgct
      601 ccgggtgcct atgcagttgt ggctgtaaac tctcaagcca tgcagaatac atttggtatt
      661 tcgaccttgc aatggagttc tggtgcattg aacaattcag gcgaactgat ccggctaaag
      721 gataaatacg ggtatactgt ggattcagtt tattatgacg acgccctgcc atgggatacc
      781 ctcgctgatg gctggggccc atctcttact ctctgtaatc cagacctcga caattccttg
      841 gcagaaaatt ggacgcacag tatggaattc gttaccgtaa atgccaatgg agatagcatc
      901 tgggctacac cgggtgggcc gtgcatcata cctgtccttc ctgttgccga tttcgttggt
      961 aacccacttt cattgatgac aggagaaagt gttcaattta ccgatctttc aactggcccc
     1021 aatattacaa actgggaatg gattttcgat ggaggaacac ctgctacctt ctcgggtcaa
     1081 aaccctccac ccatttacta ttacgaatct ggcctttatg atgttaccct taaagtaact
     1141 aacgaattcg gaacaagtac agctttcaaa cccgaatata tcactgtgtt aattgatgct
     1201 atcggcgatg acatttctct tatcccgatt tcaattcagc ctaatccttc tactggaaaa
     1261 gtaaatatta aacataacca aggcaaactc ttattggaag tatttaacct ggtaggagaa
     1321 aaagtttatt ctcaagaatt gaatccgcta actcaagacg tcgatcttac tccactccaa
     1381 aagggtatct attttatgcg attcactaat gacaatggag ttaaacttac tcacaaactc
     1441 atacttaaat aaatcaaatt ccatttattt ataaaaatag ccggggttcg gatggaactc
     1501 tggctatttt attcatttaa tcttttaaat tccagaaata ctattttctg ttcattacat
     1561 tttgatgaaa tttatcttta agtgaataaa ctctgaatag tatcatctac tgagttaaat
     1621 tttggttaag ctgtaaaaga attcagcttt tcgttttatt ttt
//
LOCUS       DAORPD010000426         1662 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_14010_length_1662_cov_2.031114, whole genome shotgun sequence.
ACCESSION   DAORPD010000426 DAORPD010000000
VERSION     DAORPD010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14010_length_1662_cov_2.031114"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(136..>1662)
                     /locus_tag="PK976_05560"
     CDS             complement(136..>1662)
                     /locus_tag="PK976_05560"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRC67386.1"
                     /translation="NQMAKHDYLSLHQADSIAQLPIDMSKYRLMDHKSGLATYFREIL
                     REELTDWCSKHYKSDGTPYNLYKDGLRIYTTINSHMQEYAEYAVRKHLSEELQPAFFR
                     QMKGQKNAPFLNDLTKEEINKIYEDAMKRSDRYIHMRQAGISKDSIRKAFQTKIPMKV
                     FSWKGDIDTVMSPMDSIRYSFGFLHAGLMSMETHTGFVKAYVGGIDYRYFKYDHVKMS
                     KRQVGSTFKPFLYTLAMQEGEFNPCSMVPNVPVTFELYDGTLWTPKNSDDAREGQMVP
                     LKWALANSVNYISAFLIKRYSPEAVIKIAQKMGITSEIPPVPSICLGTPEISLYEMTS
                     AFNTFANQGVWVEPIMLTRIEDKSGNVLESFVPRKEEAISQETAYLMVELMKGVVQSG
                     TGVRLRYKYGFDNPIAGKTGTTQNQSDGWFIGLVPQLTTGVWVGGELRSIRFRNISMG
                     QGASMALPIWAYYMKRVYADPSLGIRKDDFERPSSVPPFNFNCPAIDEDAAVYQQDEF
                     "
BASE COUNT          430 a          347 c          368 g          517 t
ORIGIN      
        1 taggatttcc atggatcttt aaataaaaag gtttgaatga aatttctggt tccatttttt
       61 taaattcttg tctctggagc atattttttc tccattttta aacaaggaat aaaaaaattt
      121 tctaattcga cttattcaga attcatcttg ttgataaact gcggcatctt cgtcgatagc
      181 ggggcagttg aagttgaagg gagggactga tgaagggcgt tcgaaatcgt ctttacggat
      241 accgagagaa ggatcggcat agacgcgttt catataataa gcccagatag gcaaagccat
      301 gcttgcgcct tgtcccatag aaatgttacg gaagcggatg ctgcgtaatt ctccacctac
      361 ccaaacgccc gtagtgagtt gaggaacgag gccaataaac catccgtcgc tttggttttg
      421 tgtggttcca gttttcccgg caatgggatt atcgaagcca tatttataac gcaggcgaac
      481 tcccgttcca ctctgtacga cacctttcat caattccacc attagatagg cagtttcctg
      541 gctaatagct tcttctttac ggggcacgaa actttcgagc acatttccgc tcttgtcttc
      601 aatgcgcgta agcatgatgg gttctaccca aactccctga ttggcaaaag tgttgaaagc
      661 acttgtcatt tcgtaaagag aaatttcggg tgttcccagg cagatggaag gaactggagg
      721 aatttcggaa gtaatcccca ttttttgtgc tattttgatt accgcttcgg ggctgtaacg
      781 ttttatcaga aaagccgaaa tataatttac ggaattagcc agagcccatt ttagaggaac
      841 catttgtcct tcgcgtgcgt cgtcactatt tttaggcgtc cagagggtcc cgtcatacag
      901 ttcgaaagtt accggaacat tgggcaccat cgagcaggga ttaaattcgc cttcctgcat
      961 ggccaaggta taaagaaagg gcttaaacgt agatcctacc tgtcgtttcg acatttttac
     1021 atgatcgtac ttgaaataac gataatctat acctcccaca taggctttca caaatcctgt
     1081 atgggtttcc atgctcatca atccggcatg cagaaaacca aacgaatagc gaatgctgtc
     1141 cattgggctc atcaccgtat ctatatcgcc tttccagcta aaaactttca tgggaatttt
     1201 tgtttggaag gcttttcgga tagaatcttt cgagatgcct gcctggcgca tgtgtatgta
     1261 acgatcggag cgtttcatgg catcttcgta aattttgtta atttcttcct ttgtcagatc
     1321 attcaaaaaa ggtgcatttt tttgtccttt catttgtcga aagaaggccg gttgcagttc
     1381 ttcactcagg tgtttcctga cggcatattc ggcatattcc tgcatgtgag aattgatggt
     1441 ggtatagatg cgcaagccat ctttatagag gttgtaaggt gtgccatcgc ttttgtaatg
     1501 tttcgagcac cagtcggtaa gttcttcccg caatatttct ctgaaatagg tagccagtcc
     1561 gcttttatgg tccatcaatc ggtatttcga catatcgatg ggcaattgag cgatggaatc
     1621 ggcttgatgt aaactaaggt aatcatgttt agccatttga tt
//
LOCUS       DAORPD010000427         1656 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_14098_length_1656_cov_2.048720, whole genome shotgun sequence.
ACCESSION   DAORPD010000427 DAORPD010000000
VERSION     DAORPD010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14098_length_1656_cov_2.048720"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1656)
                     /locus_tag="PK976_05565"
     CDS             complement(<1..>1656)
                     /locus_tag="PK976_05565"
                     /inference="COORDINATES: protein motif:HMM:NF014416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HRC67387.1"
                     /translation="ATSPKAQALAKQIAKGDKFRELLVYDSLVKAREKFFFDSIADQV
                     VYNIGVRKYTYKEVKERELNLGLDLKGGMNVTMEVSVPDILKALSNYSTDTTFNEALR
                     LAREKQKNSNADFVDLFYESFKELDPNGSLAAIFATVELKDKVKFNSSNEEVLQVLRE
                     ETKAAVDRTFNILRTRIDRFGVAQPNIQKLQTTGRILIELPGIKDPDRVRKLLQGSAR
                     LEFWPTYNFDEVYQFFDEADKRLMAIYKEEGLITPEDTIASAKNKESGITDSLQTTEA
                     DTGLLAKLADSARLQKSKQTSFEEYAKEHPLYAYLIPSYYPDENNQFHPAKTARVGSA
                     AIKDTARVNAMLKRVKDVFPSNMRLHWTVKPDPQRPGILDLVAIKVTRPDGQAPLGGE
                     VVIDARQDVNPTTGGVEVSMTMNREGAAEWKRITAENIGRQVAIVLDGYVYSYPVVQT
                     EISGGRSAISGGGMTVEEAKDLANILQSGRLPAPARIVQEEVVGPSLGKEAINAGMKS
                     FAAAFFIVLVYMFLYYNRGGLIADLALCLNLFFIFGVLASFGA"
BASE COUNT          403 a          373 c          388 g          492 t
ORIGIN      
        1 cggcaccaaa tgaggcaagt actccaaaga tgaagaaaag gttgagacac agggcaagat
       61 cggcaattaa tccgcctcgg ttgtagtaga ggaacatata aaccagaacg ataaaaaatg
      121 ctgcagcgaa ggatttcatt ccggcgttga tagcttcttt ccccaatgag ggccccacta
      181 cttcttcctg tacgatgcgt gcaggtgctg gcaaacgtcc cgactgcagg atattggcca
      241 ggtctttggc ttcttctacg gtcattccgc cgcccgaaat ggctgatctg cctcctgaga
      301 tttccgtctg aacaacggga taggaataaa catatccatc cagcacgata gccacttgcc
      361 gacctatatt ttcggctgta attcttttcc attcagcggc tccctcgcga ttcatggtca
      421 tgcttacttc cacacctccg gtggtaggat ttacgtcttg tcgtgcatct ataacgactt
      481 cgcctcccag aggagcctga ccatcaggtc tcgttacctt tatggctact aaatcgagta
      541 tgccagggcg ctgaggatcg ggtttcaccg tccagtgaag tcgcatgttc gaaggaaaaa
      601 catccttaac tctttttagc atagcattta cgcgtgcagt gtcttttatt gcagcacttc
      661 ccacgcgggc agtcttcgca ggatggaact gattgttctc gtcaggataa taagaaggga
      721 ttaaataggc atataaagga tgttctttag cgtattcttc aaaagaagtc tgttttgatt
      781 tttggagtct ggcactgtcg gctagtttgg caagcaagcc cgtatcggct tccgtggttt
      841 gcagggaatc agtgattccg ctttctttgt ttttagccga agcgattgta tcttccggtg
      901 taatcagacc ttcttcctta taaattgcca tcagtcgttt atcggcttcg tcgaagaatt
      961 gatagacttc atcgaaattg taggttggcc agaattcgag ccgggcactt ccctgaagta
     1021 attttctcac acgatcgggg tctttgatgc caggcaattc gataaggata cgccctgtag
     1081 tttgtaattt ttggatattg ggttgagcaa ccccaaagcg gtcgatacga gtgcgaagaa
     1141 tattgaaagt acggtcgacg gcagctttcg tttcctcgcg caaaacctga agaacttctt
     1201 cgttgctgga attgaacttc accttgtctt tcaactcaac cgtagcgaat atggctgcaa
     1261 ggcttccatt agggtctaat tccttaaatg attcgtagaa taagtcaaca aaatcggcat
     1321 tgctgttttt ttgtttttca cgagctaagc gcaaggcttc gttaaaagtc gtatctgtgc
     1381 tgtaattcga cagggctttc aagatgtcgg gtacagaaac ttccatggta acgttcatgc
     1441 cgcctttgag gtcgaggcca aggttgagtt cgcgttcctt tacttcctta taggtgtatt
     1501 ttctaactcc gatgttgtaa actacctgat cggcaataga atcgaagaaa aatttttctc
     1561 ttgcctttac aagcgagtca tatacaagaa gttcacgaaa tttatcgcct ttagctattt
     1621 gtttggcaag tgcctgggct tttgggctgg tggcat
//
LOCUS       DAORPD010000428         1653 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_14150_length_1653_cov_2.113892, whole genome shotgun sequence.
ACCESSION   DAORPD010000428 DAORPD010000000
VERSION     DAORPD010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14150_length_1653_cov_2.113892"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /gene="atpE"
                     /locus_tag="PK976_05570"
     CDS             complement(<1..192)
                     /gene="atpE"
                     /locus_tag="PK976_05570"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HRC67388.1"
                     /translation="MTLLSLILQAARDLSPFAKIGAGLVASIAAIGAAIGIGNIGARA
                     LEAIARQPEAANDIRSSMII"
     gene            complement(220..1323)
                     /gene="atpB"
                     /locus_tag="PK976_05575"
     CDS             complement(220..1323)
                     /gene="atpB"
                     /locus_tag="PK976_05575"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HRC67389.1"
                     /translation="MIIKEMIKKLFFFIFAIFFPFTLVKAENSPVKAAEFKPGEMIIE
                     HITDAHEWHILSIGNTHISIPLPIILWHEGKLHIFCYSKFHHGQSDYGGFRYMTDGAY
                     KNKIIYVGNNSLGNENKSPTIPLDFSITKNVASLFLSVIALIIIFLTVARAYKRNEKH
                     APKGLQSLLEPIIIFVRDDIAKSSLGEKHYERFTPYLLTVFFFIFFNNLLGIVPFFPG
                     GANVTGNITVTMMLALFTFLITNLSGNRHYWQDIFNTPGIPWWLKIPIPILPLIEFLG
                     LFTKPFVLMVRLFANMTAGHIIVLGFMSLIFVFGNINPAFGYGISLISILMSIFISLL
                     EILVSFLQAFVFTLLSALYFGMATEEIENSNHK"
     gene            complement(1468..>1653)
                     /locus_tag="PK976_05580"
     CDS             complement(1468..>1653)
                     /locus_tag="PK976_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67390.1"
                     /translation="VWGITGFVQQNLHYTDDNLDLMVQILLQNNAVNPDDILVKITGF
                     PMKERATSNMVVVTRVK"
BASE COUNT          519 a          301 c          337 g          496 t
ORIGIN      
        1 tattatcata ctagagcgaa tgtcgttggc agcctcaggt tgacgagcga tggcttccag
       61 ggctcgagct cctatgttgc caataccaat ggcggctcct atggctgcta tgctggcaac
      121 taatcccgca cctatctttg caaaaggact taaatccctt gctgcctgta atatcaatga
      181 aagtaatgtc ataattgtat tgttttaaga agatgaatgt tatttatgat tagaattttc
      241 aatttcttct gttgccatac caaagtataa tgctgataat aaagtaaaca cgaaagcttg
      301 taaaaaagaa accaggattt cgaggaggct gataaatatt gacattagaa ttgatatcag
      361 gcttattccg taaccgaagg cgggatttat gttgccaaag acgaagatca gactcatgaa
      421 acccagaaca atgatatgtc ctgccgtcat attggcaaac aaacgaacca tcaatacaaa
      481 aggttttgtg aataagccaa ggaattcgat cagaggcaaa ataggaattg gtattttcag
      541 ccaccaggga atgccgggag tgttgaaaat atcttgccaa taatggcgat tgccactcaa
      601 gttggtaatg agaaaggtaa ataaggccag catcatcgtt accgtaatat ttcctgttac
      661 attggcaccg ccgggaaaga atggaacaat gcccaaaagg ttattgaaaa aaatgaagaa
      721 aaatacggta agtagataag gcgtaaatct ttcgtaatgt ttttcaccca gcgatgattt
      781 tgcaatatcg tcgcgtacaa aaattataat gggttcgagg agtgattgta gccccttagg
      841 tgcatgcttt tcatttcgtt tgtatgccct cgcaactgtc aaaaaaatga ttatcaatgc
      901 tatgacactt aaaaataatg aagccacatt cttggtaatg gaaaaatcga gtggaatagt
      961 tggactttta ttttcattac ctaggctatt gttccccaca taaatgattt tattcttgta
     1021 agccccgtcg gtcatatatc tgaaacctcc gtaatcggat tggccatggt gaaatttcga
     1081 gtaacaaaaa atgtgcaatt tgccttcatg ccataatata attggcaaag ggatgcttat
     1141 atgggtattc cctatactta aaatatgcca ttcgtgcgca tcggtgatat gctcgataat
     1201 catttcaccc ggtttgaact cagcggcttt tacgggtgaa ttctctgctt ttaccaatgt
     1261 aaagggaaag aaaatagcaa agataaaaaa gaagagcttt ttaatcattt ctttgataat
     1321 caaataaata tcgagttttc ttcctcataa gaatgtaggt gcaaggataa tacttttttt
     1381 attatcatgc tatcatagcc aatggaatat gcatgaattc agcttttcag tagatttaac
     1441 cattgaattt caatttcaag gaatctgtta tttcactctg gtaaccacca ccatattcga
     1501 cgtggctctt tctttcatgg ggaatccggt aatcttcacg aggatatcgt cggggttaac
     1561 ggcattgttt tgaagcaata tctgcaccat gaggtcgagg ttgtcgtcgg tataatggag
     1621 gttttgctgt acgaaacccg taatgcccca aac
//
LOCUS       DAORPD010000429         1651 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_14185_length_1651_cov_1.904135, whole genome shotgun sequence.
ACCESSION   DAORPD010000429 DAORPD010000000
VERSION     DAORPD010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14185_length_1651_cov_1.904135"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /gene="xth"
                     /locus_tag="PK976_05585"
     CDS             <1..521
                     /gene="xth"
                     /locus_tag="PK976_05585"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008853 - exodeoxyribonuclease III
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HRC67391.1"
                     /translation="MGKTEYDREGRFLRADFGEISVISVYHPSGTTGEVRQDFKMQWL
                     EFFLQYVNNLRKERPYLIISGDFNICHKPIDIHDPIRNAHSSGFLPEEREWMDRFVAS
                     GFIDTFRVFNDQPHQYSWWSFRARAREKNLGWRIDYHFVTENLRPRLKNAWILPHVNH
                     SDHCPVGLEIEI"
     gene            complement(523..1140)
                     /gene="cysC"
                     /locus_tag="PK976_05590"
     CDS             complement(523..1140)
                     /gene="cysC"
                     /locus_tag="PK976_05590"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HRC67392.1"
                     /translation="MMPNTQQNLFPSSSSERNKMESLLGQKSVVIWFTGLSGAGKTTL
                     GRILRDELFSRGFIAQMLDGDNLRSGLNKDLGFSVEDRIENIRRAAEVSKLFMESGII
                     SINCFITPTEAIRAMARNIIGKENLLEVFVDCPIDICQQRDTKGLYLKAKEGLINDFT
                     GVNSPFEIPQYSDIHLFTHIMHPYRCLEVLMEYILPRITYHKTGK"
     gene            1331..>1651
                     /locus_tag="PK976_05595"
     CDS             1331..>1651
                     /locus_tag="PK976_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HRC67393.1"
                     /translation="MKLLKEKSIIWLLSIALLMPFFVKAQDTKTDSSYQFTLVKQLPY
                     TPVKNQFRSGTCWSFSAISFIESELLRKGKGEYDLSEMFCVRDAYEKKAEKYLRMGGT
                     TNFSG"
BASE COUNT          494 a          329 c          324 g          504 t
ORIGIN      
        1 gaatgggtaa aaccgaatat gatcgggaag gtcgttttct gagagccgat tttggggaga
       61 tctccgtcat aagcgtctat catccttcgg gtacaactgg cgaagtacgt caggatttca
      121 aaatgcaatg gctagaattt ttccttcaat atgtcaataa tcttcgaaaa gaaagacctt
      181 atctgattat ttcgggagat ttcaatatat gccataaacc catcgatatt cacgatccaa
      241 tcaggaatgc tcatagcagt ggattcctac ccgaagaaag agagtggatg gatcgctttg
      301 tggcttcggg ttttatcgat actttccggg ttttcaacga ccagccccat caatattcat
      361 ggtggagttt cagagcccga gcaagggaaa aaaaccttgg ctggcgcatc gattatcatt
      421 tcgttaccga aaatctcagg cctcgcttga aaaatgcatg gattcttcca cacgtgaatc
      481 atagcgacca ttgcccggta ggtctcgaaa tagagattta aatcatttgc cggttttatg
      541 atatgtgatt cgtggcagga tatattccat caaaacttca aggcatcgat aaggatgcat
      601 gatatgtgta aagagatgaa tatcggaata ctgagggatt tcaaatggtg aattcactcc
      661 agtaaaatca ttgatcagcc cctctttggc tttaagatac aatcctttgg tgtctctttg
      721 ctgacaaata tctattgggc aatcgacaaa aacttcgaga aggttttctt tcccaataat
      781 atttcttgcc atagcgcgaa tggcttcggt gggagtaata aaacaattta tgctaatgat
      841 tcctgattcc atgaagagtt tgctgacttc ggctgctcgc cggatatttt cgatgcggtc
      901 ttcgacactg aaacctaaat ccttattcaa tccgctacgt aaattatccc catctaacat
      961 ctgggcaata aatcctcttg aaaaaagctc atctctcaaa atcctcccca aagtcgtttt
     1021 gccagcaccc gaaagccctg taaaccaaat taccacgctt ttttgcccta acaggctttc
     1081 cattttattc ctctctgagg aagaggatgg gaaaagattt tgctgagtgt tcggcatcat
     1141 gatattttcc atcacaaaat tacataataa ttccaaagag tactcagagg gataaagaat
     1201 agatatttga caattatatg aaaagttttt gaatttcttt attcgcagcc aatttcatcg
     1261 tataaatttt tgtttgctga gttagagtgt ctatttttgc gaaaaatcat tattgctaaa
     1321 ttttacgacc atgaaattac tgaaagaaaa atcaatcatc tggttattga gtattgcctt
     1381 actcatgcca tttttcgtta aagctcagga cactaaaacc gatagttcct atcagtttac
     1441 tctcgtaaaa caattgcctt atactcccgt taaaaatcag tttcgttcgg gtacttgctg
     1501 gagtttctcg gctatttcat ttatcgaatc ggaactcttg cgaaaaggga aaggcgaata
     1561 cgacctttcg gaaatgtttt gtgtaagaga tgcctatgaa aaaaaagccg aaaaatatct
     1621 ccgtatggga ggaaccacca attttagcgg a
//
LOCUS       DAORPD010000430         1647 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_14235_length_1647_cov_2.856156, whole genome shotgun sequence.
ACCESSION   DAORPD010000430 DAORPD010000000
VERSION     DAORPD010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14235_length_1647_cov_2.856156"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            284..883
                     /locus_tag="PK976_05600"
     CDS             284..883
                     /locus_tag="PK976_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67394.1"
                     /translation="MAAEGGWSTRTAKIADDVPPDFKNYMKDLKTGFHFGMDAYYYFI
                     ENIGIGIKCNRYHSKNVLKNVYIQYANGSMEFGNMSDNIKIYFIGPFVSLRMLNKNKN
                     NNFYMNWGIGYTQYNDEAVFVYEFSTKGNNIGLFADWGYEIGLSKNFSLGFQLSIIAS
                     TLTKYKLITDFHTETVKLDKENYENLSHLNLSIGLRFYK"
     gene            1127..>1647
                     /locus_tag="PK976_05605"
     CDS             1127..>1647
                     /locus_tag="PK976_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67395.1"
                     /translation="MKAKIFCLSLLASLFFMAGCGEDDDGTEPTPNGDSTKISPSWTF
                     TEIPELKFAEISRNGKWAIVASDYRMALLDLKNKAELYEVATSESDLDYVVQVSISDQ
                     GYALVASANGIGVISATQGLIANFSEVYIGQARYYVGGYPSMIAPSGVSYYVHGNMKF
                     SLPDNLVWLNTTD"
BASE COUNT          555 a          244 c          284 g          564 t
ORIGIN      
        1 aaattttcga aatgttgttt gtatgatatt ccttatacct ttatgtaatt cgctgccttt
       61 aatagctcag gacttaattg ttacaataga caatgactcg ataaattgta aaataacgaa
      121 aataaaaccc gactttattt atttcacttt taagtataaa aatgaaataa ggaatacttt
      181 gttgccaaag agtcaggtaa aatatcatca atccaattac tttgccactt ccgaaatccc
      241 tcaagataaa ataaagataa tacgggattt ccctcgtttt aaattggctg ctgaaggagg
      301 atggagtaca cgtacagcaa aaatcgcaga tgatgtaccc cctgatttta agaattatat
      361 gaaggattta aaaactggtt ttcatttcgg catggatgca tattattact tcattgaaaa
      421 cattgggatc ggaataaaat gcaatcgtta tcactcaaaa aatgtattaa aaaatgttta
      481 catccaatac gctaatggaa gtatggagtt tggaaatatg agcgacaata ttaaaattta
      541 tttcataggc ccatttgtta gcctccgaat gttgaacaag aataaaaata ataatttcta
      601 tatgaattgg ggaataggat atactcaata taatgatgaa gctgtttttg tttatgaatt
      661 ttccactaaa ggaaataata taggcttatt tgcagattgg ggatatgaga ttggtttatc
      721 taagaatttt tcacttggat ttcagctctc gattatagcc agcaccttaa caaaatataa
      781 attaataact gattttcata ctgaaacggt taaactggat aaggaaaatt acgaaaattt
      841 atcccacctc aatttatcaa taggtctaag attttataaa tgaaacaggt agattttctt
      901 tccttactta caataaacct attttatttt ttctttttca ataataactg aatatataat
      961 tttctataat ttcttgagat aaagaaactt ttcaattgct taaaacttta ttttcattaa
     1021 ggtttcaaga agaaatcaaa atttttataa atttttttgt ctgtttgtaa aagtgaacaa
     1081 atctttgcca taattttacc attcaattca caaaaggagg tgcattatga aagcgaaaat
     1141 cttttgtctt agcctgttgg caagcttatt tttcatggct ggttgtggtg aggatgatga
     1201 tggcacagaa cctactccca atggagattc aactaaaata agtccatctt ggacatttac
     1261 tgaaattccc gaattaaaat ttgctgagat ttcacgtaat ggcaaatggg caatcgtagc
     1321 cagtgattat agaatggctt tattggattt gaaaaataaa gccgaacttt atgaagtggc
     1381 aaccagcgaa agcgatttag attatgttgt gcaagtttcc atttctgacc aaggttatgc
     1441 tttggtcgca agcgcgaatg gaatcggggt tatttctgca acccaaggct tgatagccaa
     1501 tttttcggaa gtttatattg gtcaggcacg ttattatgtg ggaggttatc catctatgat
     1561 tgccccttct ggggtttcat actatgtaca tggaaatatg aaattttctc ttcctgataa
     1621 tctggtgtgg ttaaatacta ccgacag
//
LOCUS       DAORPD010000431         1646 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_14262_length_1646_cov_2.440603, whole genome shotgun sequence.
ACCESSION   DAORPD010000431 DAORPD010000000
VERSION     DAORPD010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14262_length_1646_cov_2.440603"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            272..1105
                     /locus_tag="PK976_05610"
     CDS             272..1105
                     /locus_tag="PK976_05610"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HRC67396.1"
                     /translation="MKFTDFIKSKLFFRNLALMVVVTLILIWLILQSLKLYTRHGQFI
                     LLPDFTGKPLDELVQFADEKGLKVIITDSIYDENMPPRSIRVMDPQPNTGVKSGRKIY
                     VTIASGTPDLVPMPDIRELSLRQAVETLVLSGLRIDYISYVEGPFQNSVIGQRIGTRN
                     VNPGELLPRNSKIVIVVEKGDNPVPAVVPDVIGLHPYEAYNTLFRSGLNLGKISFPDG
                     IPQDSAMVIHQEPGIETRLTPGSYVSLVFGQIKDAKQLKSLRQHHQNRDSIIDENEEE
                     F"
     gene            1123..>1646
                     /locus_tag="PK976_05615"
     CDS             1123..>1646
                     /locus_tag="PK976_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67397.1"
                     /translation="MKIDIEHWFLRSRMLKIIRNILLLLLCFLLKPLIAQEILSGLEY
                     NPAVGRESKVLLLKKTNQETSSLLLPFFDDFSKSEVFPDPNLWDDHNVYVNQGFAFNP
                     PNYRVVTLDALDSTGKVYAHATHTPFEADRFTSRPIRLDSIFSPAPRALRKADSIYLS
                     FYFQPQGHGNPPQK"
BASE COUNT          494 a          338 c          312 g          502 t
ORIGIN      
        1 cccctcccac aaccgctata tttagtttag gcattgttct ataaaattta gcatgcgaaa
       61 ttacaaaaac attgttcagc tataggatta gaaattttct ttcgaaccta aagatcataa
      121 agaatgaaac ggccggaaaa ccttaaaaat gacagattat tgatactttt gcaaacctga
      181 gcaataaagt cattcggata aagtttctat gaaagtgaat gggaaatacc ctcttatatc
      241 gtcctatcaa ttataataag aacatcctgt gatgaaattt acggatttta tcaaatctaa
      301 gttgtttttc cggaatctgg ccttgatggt ggtagttaca ctgatactca tctggttgat
      361 tctgcaatcg ctgaagttat acacccgtca tggtcaattt attctattgc ctgattttac
      421 gggtaaacct ttggatgagc tagttcaatt tgccgatgag aaaggactca aggttattat
      481 tactgactcg atatatgacg aaaacatgcc accacgaagc attcgggtaa tggatcctca
      541 accgaataca ggtgtaaaat caggccgaaa aatatatgta accatagcgt caggtacacc
      601 cgatttagtt cccatgcccg atatcagaga gctgtcattg cgtcaagccg tcgaaacact
      661 tgttctttct ggtttacgaa tagattacat ttcctatgtg gaaggtcctt ttcaaaattc
      721 ggtaattggt caacgtatcg gcacgcgtaa tgtaaatcca ggcgaattgt tgccaagaaa
      781 ttccaagatc gtcattgtcg tcgaaaaagg ggataatcca gtacctgccg ttgttcccga
      841 tgttatcggc ttacaccctt acgaagctta taatactttg tttcgctcag gcctaaatct
      901 cggaaaaatt tcttttcccg atggcattcc acaggattca gccatggtaa tacatcagga
      961 gccaggtatt gagactcgac ttactcccgg ttcgtatgta agcctggttt ttggccagat
     1021 aaaagatgcc aaacaactga aatcacttcg tcaacatcat caaaacagag atagcatcat
     1081 tgatgaaaac gaggaagaat tttaaaattt gaagtcaaaa aaatgaaaat agatatcgaa
     1141 cactggtttt tgcgaagtcg aatgttgaaa ataatcagaa atattctgtt gctcttattg
     1201 tgttttttgt tgaagccatt gatagctcag gagattttat cgggcttgga atataatcct
     1261 gctgttggca gagaatcgaa ggtccttctt ttaaaaaaaa ccaatcaaga aacatcctcc
     1321 ttgcttctgc cttttttcga tgattttagt aagtcggaag ttttccccga tcctaatctg
     1381 tgggatgacc acaatgtcta tgtaaatcag ggctttgctt tcaatccacc taattacagg
     1441 gtggttacac tggatgcatt agattctacg ggaaaggttt atgctcatgc cacccatact
     1501 cctttcgaag ccgatcgatt tacttcgaga ccgattcgac tcgattctat tttttcaccc
     1561 gctccgcgtg ccttgcgtaa agccgattcc atttatttaa gcttctactt tcaacctcaa
     1621 ggccatggca atcctcctca aaaaaa
//
LOCUS       DAORPD010000432         1643 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_14323_length_1643_cov_1.701511, whole genome shotgun sequence.
ACCESSION   DAORPD010000432 DAORPD010000000
VERSION     DAORPD010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14323_length_1643_cov_1.701511"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="PK976_05620"
     CDS             complement(<1..693)
                     /locus_tag="PK976_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67398.1"
                     /translation="MKKLFLAIMAFSVNLITGFHLEAQNYQHFETPDNSSPKCYVDLI
                     ILENDNAQLVLDMPSSAELDFNSPTIFYGKIDKNTGEASLQSMNEKLWLNFLYTQKQL
                     KNPKLNQGQKQLYLPSFNLTWDRGNLPLKVIHQNKKQPLFSMKETPKAVVDIIFPLPQ
                     YSINPRINDSIMFHLFMSYFGQEYPKGKTILQAIDNQIDKFCKSYITENQEYYDRSLP
                     DAAFNWERTYSFQ"
     gene            complement(807..>1643)
                     /locus_tag="PK976_05625"
     CDS             complement(807..>1643)
                     /locus_tag="PK976_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC67399.1"
                     /translation="QKLGHVSKPILICLIMLCIGFISFFIFTFMDKKLDASQEAYDKD
                     NEVVANEESFKVSDILKIISNRGWWFIAILCVLFYSAVFPFLKYAADMMVNKFGIDQS
                     IAGTIPAVLPFGTILLTPLFGNLYDRKGKGATIMIIGAILLIFVHAIFSLPFINQWII
                     AVLLIIVLGIGFSLVPSAMWPSVPKIIPEHQLGTAYALIFWVQNWGLMGVPALIGWVL
                     DKYCVTGQKLVEGTLVNTYNYTLPMIIFTTLGVLALIFGFLLKAEDKRKGYGLQLPNI
                     QK"
BASE COUNT          542 a          265 c          331 g          505 t
ORIGIN      
        1 ttgaaatgaa taagttcttt cccaattaaa agcagcatcg ggtaaagatc tatcataata
       61 ttcctgattt tctgtaatat atgatttaca aaatttatct atttgattat caatggcttg
      121 taagatagtt ttaccttttg gatattcttg accaaaatag ctcatgaaca aatgaaacat
      181 gatagaatca tttattcgtg gatttatgga atactgaggc aaagggaaaa tgatatcgac
      241 aacggcctta ggggtttctt tcatgctaaa taatggttgc ttcttgtttt ggtggataac
      301 tttaagtggt aagttaccgc gatcccatgt caggttaaaa gaaggcagat acaactgttt
      361 ttgaccctgg tttaatttag gattcttcaa ttgtttttgg gtatataaaa aattcagcca
      421 aagtttttcg ttcattgatt gaagagaagc ttcacccgta tttttatcta tctttccata
      481 gaaaatggtt ggggagttga agtctaattc tgctgatgag ggcatatcta ggacgagttg
      541 ggcattatca ttctctagaa taatcaaatc aacataacat ttcggagatg agttatcagg
      601 tgtttcgaaa tgctgatagt tttgggcttc gagatgaaat cccgtaatta agttaacgct
      661 aaacgccata atggcaagaa ataatttttt cattgtcgta gcctccattt ggttattttg
      721 gaaataaggt ttctaaaaaa catatacatg aaaatggaga aagagagggg tcattcgatg
      781 aatacccctc tcctatttta tgatagttat ttttggatat ttggaagttg gagaccatag
      841 ccttttcgtt tgtcttcagc tttgaggaga aaaccgaaaa taagagctaa aacacccaga
      901 gtagtaaaga tgatcatggg gagagtataa ttgtatgtat ttaccaaagt tccttccact
      961 aatttttgac ctgtaacgca atatttatcg agtacccagc caattaatgc aggaacgccc
     1021 atcaagcccc agttttgaac ccagaatatc aaggcatagg ctgtacccag ttgatgttcg
     1081 gggatgattt taggaacgga aggccacata gctgaaggca ccaaagagaa acctattcca
     1141 agaacaatga ttaaaagcac ggcaatgatc cattggttaa tgaagggcaa tgaaaatatg
     1201 gcatgtacaa agataagtaa aattgcacca ataatcatga ttgtagcacc tttcccttta
     1261 cggtcgtata gattgccaaa taaaggtgtt agcaggattg tgccaaaagg taatactgct
     1321 ggaattgtgc cggcgataga ttgatctatt ccgaatttat ttaccatcat gtcggctgca
     1381 tatttaagga aagggaaaac tgccgaatag aagagcacgc agagaatagc aataaaccac
     1441 catccacggt tactgatgat tttcagaata tcagaaactt tgaatgattc ttcgttggct
     1501 actacctcat tatctttgtc ataggcttct tgagaagcat cgagtttttt atccatgaaa
     1561 gtaaagatga aaaaagagat aaaaccgatg caaagcataa tcaaacagat aagaataggt
     1621 ttcgaaacat gacccaattt ttg
//
LOCUS       DAORPD010000433         1642 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_14333_length_1642_cov_2.197227, whole genome shotgun sequence.
ACCESSION   DAORPD010000433 DAORPD010000000
VERSION     DAORPD010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14333_length_1642_cov_2.197227"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /locus_tag="PK976_05630"
     CDS             complement(<1..925)
                     /locus_tag="PK976_05630"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HRC67400.1"
                     /translation="MNSIIVAFHTLGCKLNFAETSTLIRKFRELGYQITDFDKEADIY
                     IIHSCSVTSVAEKKSRAAIYQAHRRNPKAFIAVIGCYSELEAKKLKAISGVQWVLGNQ
                     EKFRITELISRDFSTNLEVSVTSLDSEPSKNLQEFHPAYSSGDRTRSFLKIQDGCDHF
                     CSYCSIPYARGKSRSPSVQEALQWAQKAAADGVKEIVLTGVNIGDFGRKNGESLYDLL
                     QLFDKGRIVKRVRLSSIEPELLSDDIIRLIAESRVLMPHFHIPLQSGSDTILKRMKRK
                     YTTKVFESRVKYIKQLMPYACIACDIIVGFHG"
     gene            complement(970..>1642)
                     /locus_tag="PK976_05635"
     CDS             complement(970..>1642)
                     /locus_tag="PK976_05635"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRC67401.1"
                     /translation="LNPFLYYLILLKAYELLPAQEAGTLNYFWPVVLVLLSIPMLGQK
                     ISWMSIISVFISFIGIVIISTHGKPWLMQFSNPLGVVLALTCPFIWAIYWILNMKIAG
                     DEVEKLFIGFLSGTILIIIYSAIKHSLTFPPIKGLAGAIYVGIFEMGLTFIFWLKALK
                     YSKTTAHISNLVFLSPFLSLFYINFILKERIMLSTITGLILVVVGIIMQQYVPDIEKK
                     GRKAQ"
BASE COUNT          524 a          327 c          296 g          495 t
ORIGIN      
        1 cgccatggaa gcctacaatt atatcgcagg caatacatgc ataaggcatt aactgtttaa
       61 tgtatttaac ccgactctca aagactttgg tcgtgtattt tctcttcatt ctttttagta
      121 ttgtatccga gccggattgc aaaggaatat ggaaatgtgg cattaaaacc cgtgattcag
      181 ctattaatcg aatgatatca tcgctcaata actctggctc gatggacgaa aggcgaactc
      241 tctttacaat tcttccctta tcgaaaagtt gcagcaaatc atataatgat tctccatttt
      301 ttctaccaaa atcaccaata ttaacgcccg taagaacgat ttcctttact ccgtctgctg
      361 cggctttttg cgcccattga agagcttctt gtacagatgg actgcgcgac ttacctcgag
      421 cataaggtat ggaacaatag gaacagaaat ggtcgcaccc atcttgaatc ttcagaaagg
      481 aacgagtcct atcgcccgag gaataagcag gatgaaactc ttgcaagttt tttgaaggtt
      541 ctgaatctaa agatgtaacc gaaacctcga ggtttgttga aaaatcacgg gaaatcagtt
      601 cggtaatccg aaatttttcc tgatttccca aaacccactg cactccgctg atcgctttca
      661 atttttttgc ctcgagctcg gagtaacatc caatcacagc tataaaagcc tttggatttc
      721 gccggtgagc ctgatatatg gctgcacggc ttttcttttc agccaccgat gtaacactgc
      781 aggaatgtat gatatagata tcggcttctt tatcgaaatc agtaatttga taaccaagtt
      841 ctcgaaattt gcgtatcaaa gtggaggttt ctgcaaaatt caatttgcat cccaatgtat
      901 gaaaagcaac tattattgaa ttcatgcggc aaaattaata tattcctttc tatcgattct
      961 tctttatttt cactgagctt ttctcccttt tttctctata tcaggtacat attgctgcat
     1021 gatgatacct actacgacca aaatcaagcc tgtgatggta cttaacatga tcctttcctt
     1081 caatataaaa ttgatataga acaatgaaag aaatggagaa aggaaaacca gattgctgat
     1141 atgagctgtt gtttttgaat attttaaagc ttttagccaa aagatgaaag tgagtcccat
     1201 ttcgaaaatt cctacataaa tagctccagc caatccctta ataggaggga aagtcaagct
     1261 atgtttgata gccgaataaa tgataatgag aattgtacca cttaaaaaac caataaaaag
     1321 tttttcgacc tcatcgccag ctatcttcat atttagaatc caatatattg cccatataaa
     1381 aggacaggta agagctagta caacccctaa aggattactg aattgcataa gccaaggttt
     1441 tccatgggtg ctgatgatga caattcctat aaaactgatg aaaactgaaa ttatgctcat
     1501 ccagcttatt ttttgaccaa gcatgggaat ggaaagcaaa accagaacta caggccagaa
     1561 ataatttaag gttccggctt cttgagctgg cagtaactca taagctttta atagaattaa
     1621 ataataaagg aaagggttta at
//
LOCUS       DAORPD010000434         1641 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_14349_length_1641_cov_2.554224, whole genome shotgun sequence.
ACCESSION   DAORPD010000434 DAORPD010000000
VERSION     DAORPD010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14349_length_1641_cov_2.554224"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /locus_tag="PK976_05640"
     CDS             complement(<1..900)
                     /locus_tag="PK976_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018027989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HRC67402.1"
                     /translation="MNNTKKTTGKVSGIIANLVIVQVEGPVAQNEICYISHDGTDLMA
                     EVIKIIGNKAYTQVFESTRGLKIGSPAIFEGHMLEATLGPGILSSVYDGLMHDLHEMT
                     GIFLKPGEYTRALDPEKIWDFKPLAKVGDQVIAADWLGEVIENSIPHRIMVPFVFEGR
                     YTVKSIVPHGLHKIYDTIAVLTDESGKDHPVTMVQYWPVKQAIKAYKEKPRPFKLFQT
                     GVRTMDTLNPMAEGGTGFIPGPFGSGKTVLQHALSRQAEADIVIVAACGERANEVVEL
                     FVEFPQLEDPRTGRKLIERTTIIA"
     gene            complement(903..>1641)
                     /locus_tag="PK976_05645"
     CDS             complement(903..>1641)
                     /locus_tag="PK976_05645"
                     /inference="COORDINATES: protein motif:HMM:NF022415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2764 family protein"
                     /protein_id="HRC67403.1"
                     /translation="FKLELRHHLSAFDYKLVELILLPIDNKNLLHLLLKTNEEFIPNG
                     KYSLEILEEEMKNPKILPDYLRNFILHFKEEQPVYPELSWEDQLTAIYLEYAAQTKNK
                     FVSNWIKFERNVRNIMAALTARKFKLNARSFIIGNDEFSQTLIKSQARDFGLAQQFPY
                     IEKLIQIFETNNLLEREKSLDLLRWSYLDELNTFHYFSIEIILAFILKFQMVQRWLRL
                     DKEAGLAIFRRLLEDLENSYEFPKEFS"
BASE COUNT          466 a          342 c          329 g          504 t
ORIGIN      
        1 ggcaatgatg gtagtccttt cgatcagttt acgtcccgtt cttgggtctt ctaattgggg
       61 aaattctacg aagagctcga ccacttcatt ggcacgttcg ccacaagcgg cgactataac
      121 tatgtcggct tcggcttgac gcgacagcgc gtgttgcaat acggtcttgc cgctaccaaa
      181 gggtcccgga ataaaacccg tacctccttc ggccatagga ttcagagtat ccatggtacg
      241 aacgccggtt tggaaaagtt tgaaaggtcg gggtttctcc ttgtatgcct ttatagcctg
      301 ttttacaggc caatattgaa ccatggtaac gggatggtct ttcccacttt catccgtaag
      361 cacggcaatg gtatcataaa tcttatgaag accatgagga acaatggatt taacggtata
      421 tctcccttcg aaaacgaaag gcaccataat gcgatgaggg atggaatttt ctatcacttc
      481 gcccagccag tcggcagcta tcacctgatc tccgactttt gccagaggct tgaaatccca
      541 aatcttctca ggatctaaag cacgtgtata ttcaccgggt ttaagaaaaa tccctgtcat
      601 ttcatgtaaa tcgtgcatca atccatcata aacactcgaa aggattccag ggccaagggt
      661 ggcttcgagc atgtgtcctt cgaatattgc tggtgagccg attttcaatc cacgggtgct
      721 ttcgaaaact tgagtatatg ccttattacc gataattttt attacttctg ccataagatc
      781 ggttccatca tggctgatat aacaaatctc attttgtgca acaggccctt caacctgcac
      841 aatcaccaaa ttcgcaataa ttccggaaac ctttccggta gtttttttcg tgttattcat
      901 tatcatgaaa actctttagg aaattcatag ctattttcca aatcctcgag caaacgtcta
      961 aatatggcta agcctgcttc tttgtcgagc cgcagccatc tttgtaccat ttggaacttt
     1021 aatataaatg caaggattat ctctatactg aaataatgaa aagtattcaa ttcgtcaaga
     1081 taagaccagc gcaaaagatc gagcgatttt tccctttcta ataaattgtt ggtttcaaaa
     1141 atctgaataa gtttttcgat ataagggaat tgttgtgcaa gtccaaaatc tcttgcctga
     1201 cttttaatga gggtttgcga aaattcgtca ttgccaataa tgaacgaacg agcattgagt
     1261 ttaaattttc tggcagtaag cgccgccatg atatttctaa cattcctttc gaattttatc
     1321 caattcgaca cgaatttatt cttggtttga gcagcatact caagatatat ggccgtcaat
     1381 tgatcttccc agcttagctc gggataaaca ggctgctctt ctttaaaatg aagaataaag
     1441 tttcgaagat aatcggggag aatcttggga ttcttcattt cttcttcgag tatttctaaa
     1501 gaatactttc cgttaggaat aaattcttca ttggttttca aaaggagatg aagaagattt
     1561 ttattatcga tgggtaaaag aatcaattca acgagtttat agtcgaaagc cgagagatga
     1621 tgccgcaatt cgagtttgaa t
//
LOCUS       DAORPD010000435         1640 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_14369_length_1640_cov_2.372240, whole genome shotgun sequence.
ACCESSION   DAORPD010000435 DAORPD010000000
VERSION     DAORPD010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14369_length_1640_cov_2.372240"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1640)
                     /locus_tag="PK976_05650"
     CDS             complement(<1..>1640)
                     /locus_tag="PK976_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67404.1"
                     /translation="LTNMSDNDLEGNIRLEFFDLLSDKPLHLIAKGDSSVKIFSTRAG
                     LSDQYEWKIIVPSGTSVLRYRLTAQCESHSDALEDLIPVLSDQIWLGDAHPLFAAASQ
                     TTDFEISMNPEILHDSRTHITFEFTTNPAWIVLQALPSISLPERQSVDAWFASFYANA
                     IGKNIIEKNPSIRPVIEAWKRYQPSALQSNLQKNEDLKNIVLNETPWVGQAETESASK
                     NQLADFLDENKMNNDLQIAFQKISQMQSSNGGFNWFEGMPDSRYITLEIVKGLSRLLH
                     QQMIDIEDYDGLDKLLSSAVDYLDQRVLEDYKNELEHLVRDKNYQGQLSYTMAKYLYA
                     RSLLVNAFPIDKKYKESWDYYMNLARRDWIKQSLGTQALLAISFFENKEDRQARTILK
                     SLRDRALFDNEKGLYWVRKHNRWMAEGELETQAAMIEAFMYDESYYSDVENMKKWLIY
                     KKRTQNWGTSMATTEAVYALLLKGKPLLNENKPVKITVNGESLLANESGDLAIEAGSG
                     YFRKTWTNSQNIPNIMKIHIENLNDIPIWGGMYVQYFKP"
BASE COUNT          462 a          347 c          313 g          518 t
ORIGIN      
        1 ggcttgaaat attgaacgta cattcctccc caaataggta tatcattgag attttcgata
       61 tgaatcttca ttatgttggg aatattttga gaatttgtcc atgtcttcct gaaataccca
      121 cttccagctt caatggctaa atctcccgat tcgtttgcca aaagactttc accgttaaca
      181 gtaattttta ctggcttatt ttcattcaaa agaggtttcc ctttcaagag cagggcataa
      241 accgcctcag tcgtagccat ggaggttccc cagttttgag tccgtttttt ataaatcaac
      301 cattttttca tgttctctac atccgaataa tagctttcat catacataaa ggcttctatc
      361 atggcagctt gggtttccaa ttcaccttcg gccatccatc tattgtgttt tcgtacccag
      421 taaagtcctt tctcattgtc gaaaagagcc cggtcgcgga gcgatttcaa aattgttcgc
      481 gcttgcctat cttccttatt ttcgaagaaa gatatggcca gtaaggcttg ggtgcccaag
      541 gattgtttta tccagtcgcg cctggccaaa ttcatataat aatcccatga ttccttatat
      601 tttttatcaa taggaaaagc atttaccaat aaacttctgg catataaata tttagccata
      661 gtataactca actggccttg ataattttta tctctaacca aatgttcgag ttcattctta
      721 taatcttcta acactcgttg gtcgagataa tccactgccg acgaaagaag cttgtccaat
      781 ccatcataat cttcaatatc aatcatctgt tgatgtagga gcctgctcaa acctttcaca
      841 atttccaaag taatatatct tgaatcgggc ataccctcga accagttaaa tccgccattg
      901 gatgattgca tttgactaat cttctgaaaa gctatctgca agtcattgtt cattttattt
      961 tcatcgagaa aatcggctaa ttgattttta gaggcagatt cagtttcggc ttggcctacc
     1021 catggcgttt cattgagcac aatatttttc aaatcctcat ttttctgaag attcgactgc
     1081 aaagcagacg gctgatatct tttccatgct tcaataacag gccggatgga aggatttttc
     1141 tcgataatgt ttttcccgat ggcattggca taaaatgagg caaaccatgc atcaaccgat
     1201 tggcgttcgg gcaaagaaat ggagggcaag gcttgaagta caatccatgc aggatttgta
     1261 gtaaactcga aggttatatg ggttcgacta tcatgcaaaa tttcaggatt catagagatt
     1321 tcgaaatcgg tagtttgcga agcggcagca aataaaggat gcgcatcgcc aagccagatt
     1381 tggtcagaca gaacaggaat caggtcttcg agagcatcac tatggctttc acattgcgca
     1441 gtcaagcgat agcgcaaaac acttgtgcct gagggaacta ttatcttcca ttcgtattga
     1501 tcacttagtc ctgctctggt cgaaaaaatc ttgacagagc tgtcaccttt cgctattaaa
     1561 tgaagaggtt tgtccgaaag taaatcgaag aattcgagac gaatgttgcc ttccaagtca
     1621 ttatcggaca tgttggtgag
//
LOCUS       DAORPD010000436         1640 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_14370_length_1640_cov_2.203785, whole genome shotgun sequence.
ACCESSION   DAORPD010000436 DAORPD010000000
VERSION     DAORPD010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14370_length_1640_cov_2.203785"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..774)
                     /locus_tag="PK976_05655"
     CDS             complement(25..774)
                     /locus_tag="PK976_05655"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRC67405.1"
                     /translation="MSFNKVLLISFSFIFIFTIENSFAQNTGQGGQSNPSNTKGNLPS
                     QKTGVIKGRIIDQSSKMPLEYANIAIFRKRDSALINGTITNEKGDFIIEKLPFGRFFV
                     KLSFIGYATRSIDSVFITPKQPEVNLGTIRIKLVENKLQGVEITEKRAPVEYNLDKKV
                     YNVDQNIVSMGGTAIDIMQTIPAVQVDLDGNVSLRGSSNVNILIDGRPSNLVSLDQLP
                     SSMVERVEVVTNPSARYDPDGTTGIINIIGQ"
     gene            955..1110
                     /locus_tag="PK976_05660"
     CDS             955..1110
                     /locus_tag="PK976_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67406.1"
                     /translation="MSVPSDSLSWESKDKDSSPETWGNTSSPFGFLQVADKHALKRNL
                     VTCDKHL"
     gene            complement(1358..>1640)
                     /locus_tag="PK976_05665"
     CDS             complement(1358..>1640)
                     /locus_tag="PK976_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67407.1"
                     /translation="NELFTSLESSENFIPLSGYQSQLAFYFASHRTCIQTESHGIQNM
                     IPNPIDFAEIAGNRIHHQVCEAPRYLRCRLILLHATAKAADKASKWIIS"
BASE COUNT          451 a          295 c          340 g          554 t
ORIGIN      
        1 tattttggag atgccaattc cgttttattg cccaatgata ttgatgatgc cagtagttcc
       61 atcggggtca taacgagccg aagggttggt aacaacttcg acccgttcga ccatacttga
      121 gggtaattgg tcgaggctga ccaagttgct cggtctgccg tcgattaaga tattgacgtt
      181 actcgacccc ctcaggctta catttccgtc gagatcgacc tgcaccgcag gaatagtctg
      241 catgatatct atggctgttc ctcccatact tacaatgttc tgatcgacat tgtaaacctt
      301 tttatcgagg ttgtattcta cgggtgctct tttttctgtg atctcaaccc cttgaagctt
      361 attttcgacc agcttgattc ttatagttcc taaattgact tcgggttgct ttggagtaat
      421 aaatacagaa tcaatgctac gagtagcata tcctatgaat gaaagtttga cgaagaaacg
      481 gccgaatggg agtttttcga tgataaaatc gcccttttcg ttagtaatgg ttccattgat
      541 aagcgctgaa tcgcgtttgc gaaatatggc aatgttagca tattcgagtg gcatcttcga
      601 gctttgatct atgatacgtc ctttaatgac ccctgtcttc tgactgggaa gattcccttt
      661 cgtattactt ggattacttt gccctccttg ccctgtgttt tgtgcaaaag aattttcgat
      721 cgtaaaaata aaaatgaatg aaaagctgat taataatact ttgttgaatg acataaattt
      781 caatatttta atttcctcta tttgagggaa aaagaattaa ttggtttaat agagaaagta
      841 ataattttac tacactattg ctgattcatg acataaggca aatcgttttc ttcaaaaatt
      901 cttgttcaga gaacgaagta caattggctt tatattatcg atgaatttgt cttcatgtcg
      961 gttccttctg attctttatc ctgggaatcg aaggataaag atagtagccc tgaaacctgg
     1021 ggcaatacat ccagtccatt tggtttttta caggtggccg ataaacatgc cttgaaacga
     1081 aatcttgtga cttgtgataa gcatttgtaa tatctttttg tgaactaaca ttactcgagc
     1141 tttagtagta accttcgggg atattacaag ttgtcttctg ctcaaaaatc cgacttcacc
     1201 atgtttatct atttatattt aattgtgttt tagattgttt gactactcct gcattggtaa
     1261 aacgattgct tttacgatca acataatcgg actgggataa caaacgaagt tattatttgt
     1321 gagagaataa ccttggggtt ttcattagtg atttctttca ggagattatc cattttgaag
     1381 ccttatctgc tgctttagca gttgcatgca gcaagatcaa tctacatctg agatagcgag
     1441 gggcttcaca tacctgatga tgaatacgat tgccggctat ttcggcaaaa tcgattgggt
     1501 tcggaatcat attttgtatt ccgtgtgatt cagtttgaat gcaggttcga tgggatgcaa
     1561 aatagaaggc aagttgcgat tgatatccag aaaggggtat gaaattttcc gaagattcca
     1621 gggaagtgaa gagctcatta
//
LOCUS       DAORPD010000437         1638 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_14403_length_1638_cov_2.512318, whole genome shotgun sequence.
ACCESSION   DAORPD010000437 DAORPD010000000
VERSION     DAORPD010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14403_length_1638_cov_2.512318"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="PK976_05670"
     CDS             complement(<1..648)
                     /locus_tag="PK976_05670"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HRC67408.1"
                     /translation="MYSKEQILTALRQVIDPDLKNDIVTLGMVENLRIEENKVGFQLV
                     LTTPACPLKGKFQKECIEAIHQKVDPNLIVEVEMISRVTSTRKKAVESLSGVKNIIAI
                     ASGKGGVGKSTIAANLAVALARTGASTGLLDADIYGPSQVMMFGLNNIRPGVTQLNGR
                     DLIEPVEKYDVKILSIGFFVDPDKALIWRGPMASGALTQLFIDANWGELDYLVIDM"
     gene            complement(695..1240)
                     /locus_tag="PK976_05675"
     CDS             complement(695..1240)
                     /locus_tag="PK976_05675"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HRC67409.1"
                     /translation="MNALEEEKQQIRKFIKACKAKLTIEEIRLCSQLILKNIESLPAF
                     QKAETLMAYWALPDEVQNHDFILRWYQHKKIILPAVDGSHLRLKVFNGINNLAQGQCF
                     GIEEPIGEDFAHPEKIELVIVPGIAFDISNNRLGRGKAYYDKFLRSLQTLKIGICFPF
                     QLLPRIPVGPDDIKMDMVISC"
     gene            complement(1325..>1638)
                     /locus_tag="PK976_05680"
     CDS             complement(1325..>1638)
                     /locus_tag="PK976_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HRC67410.1"
                     /translation="SLGQGRVWSGVDAKNIGLIDDYGGLQYAIDEAARMAGIENYKVI
                     EYPETKHPLTQIMESFGKSKMQKAMKNQVGPYFNHFITWQNLTQIKGPIARLPYDIYF
                     E"
BASE COUNT          470 a          328 c          293 g          547 t
ORIGIN      
        1 catatcaata accagataat caagttcgcc ccaatttgca tcaatgaata actgcgtaag
       61 ggctcccgag gccataggcc ctctccagat caatgcctta tcggggtcaa caaaaaaacc
      121 aatggaaaga attttaacgt cgtatttttc gacaggctcg atcaaatcac gtccattgag
      181 ctgtgtaaca ccaggtctta tattattcaa tccaaacatc atcacttgtg aagggccata
      241 aatgtcggca tcaagaagac cggtgcttgc tcctgtacga gccagagcta ctgccagatt
      301 ggctgctatc gtacttttgc ccacaccacc tttccctgat gcaatggcta tgatattctt
      361 tacacccgac aaagattcta cggctttttt acgtgtactc gttaccctcg aaatcatctc
      421 gacttcaact atcaaattgg gatctacctt ttgatgaatt gcttcgatac attctttctg
      481 aaatttccct ttcaatggac aggctggagt tgtcaaaacc agttgaaatc caactttatt
      541 ttcttcaatt cggaggtttt caaccattcc gagggttaca atatcatttt taagatcagg
      601 atcaattacc tgacgtaagg cggtgagaat ttgttctttt gaatacataa aactttattt
      661 gatttaatca ttcttaatca gatttaggct gaattcagca tgaaattacc atatccatct
      721 taatatcatc aggtcctacc ggaattcgag gcaataattg aaaaggaaag caaatgccaa
      781 tcttaagagt ttgcaaagat cgaagaaatt tgtcataata agccttccca cgtccaaggc
      841 gattgtttga gatatcgaac gctattcctg gaacgatgac aagctcgatt ttttcgggat
      901 gtgcaaagtc ttcgccaatg ggttcttcaa tgccaaagca ttgaccttga gctaaattgt
      961 ttattccgtt aaatactttt agcctcagat gagaaccatc tactgcaggt agaatgattt
     1021 ttttgtgctg ataccatcga agaataaaat catggttttg cacttcatcg ggcagagccc
     1081 aatatgccat caatgtttca gctttttgga atgcaggcaa tgactcaata ttttttaaaa
     1141 taagttgact gcaaagtcgg atttcttcta tggttaattt tgccttacag gctttaatga
     1201 attttcttat ctgttgtttt tcttcttcga gtgcattcat aaatcttttt aaaagacgaa
     1261 actatgagga attaaggatt tattatttcg tatggttgtt ggtcaatata atttttgtcg
     1321 atgatcattc gaaataaata tcataaggta aacgagcaat agggcctttt atttgagtta
     1381 aattttgcca tgtaataaaa tgattgaagt aaggacctac ttgatttttc atggctttct
     1441 gcattttgct tttgccaaat gattccataa tttgtgttaa aggatgtttg gtctcaggat
     1501 attctatcac tttgtagttt tcgatacctg ccatacgggc ggcttcatct atggcatatt
     1561 gaagacctcc ataatcatcg atcaatccaa tgtttttggc atctactcca ctccatactc
     1621 ttccttgtcc gaggctat
//
LOCUS       DAORPD010000438         1636 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_14435_length_1636_cov_1.697027, whole genome shotgun sequence.
ACCESSION   DAORPD010000438 DAORPD010000000
VERSION     DAORPD010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14435_length_1636_cov_1.697027"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..1199)
                     /locus_tag="PK976_05685"
     CDS             complement(123..1199)
                     /locus_tag="PK976_05685"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HRC67411.1"
                     /translation="MGLIKIPGEVFVAFGLDKSCSMVRPFGSGHIHQTFRVDTDNQCF
                     IFQCINHKVFPDVAALMDNIFKVTRYISKNSSPEDLTVRIIQTLEGKSFYHDDQGQYW
                     RVMERVPNSKTYDMALSPSMAFEAGKVLGDFHYLLKDFPVEQLHTILPNFHNLDSRWR
                     EFLIAKGKASTHRLSQANALIQIVDIACDDELIFSRKIYSPHTRKRIVHNDPKINNIL
                     FDNHLRGICMIDLDTMMPGSFLHDFGDALRTTANPFYEDEPDSTSVHCDLDLLKAYCQ
                     GYLPCVKKIMNTDEIMGLPHAPAHMTFIIALRFLTDYLNDDIYFKIDHPQHNLQRAKA
                     QFALYYDFKEREPEIEQIISQYVK"
     gene            complement(1190..>1636)
                     /locus_tag="PK976_05690"
     CDS             complement(1190..>1636)
                     /locus_tag="PK976_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HRC67412.1"
                     /translation="SRTLSDYGGVSRGICQIDSNGYLKKIIETHQIHKTHGGYAYPNS
                     GGDLVFLPGDTIVSMNTWAFCPEIFQDFERLFLEFLKENHHSTSAEFYIPSVADHLIS
                     TGKARFKVLTVNEQWFGITYQEDRSKVVENLRKLVSEGYYPEPLWD"
BASE COUNT          495 a          322 c          327 g          492 t
ORIGIN      
        1 ctgaatttta attatgaaga caaaaatggt gatgggtttt gaaggtcgct ccctataata
       61 gttttcctaa agatttgaat gaagcaattg attaacagtt taatacccct aattcagtaa
      121 aatcatttta catactgaga tattatttgc tctatttcag gttctctttc tttaaaatca
      181 taatacagcg caaactgtgc ctttgctctt tgaaggttat gttgaggatg atctatctta
      241 aaataaatat cgtcattcag ataatcggta agaaaacgca atgcaataat aaatgtcatg
      301 tgagccgggg catgaggcaa acccatgatt tcgtcggtat tcataatttt tttaacacag
      361 ggcaaataac cttgacagta agccttcaat aaatccaaat cgcaatgaac cgatgtgctg
      421 tcgggttcat cttcgtagaa aggattggct gtggtgcgca aggcatcgcc aaaatcgtgt
      481 aaaaaactac ctggcatcat tgtatctaga tcgatcatgc agattcctct taaatgatta
      541 tcaaagagaa tattattgat tttagggtca ttgtgcacaa ttctttttcg agtatgggga
      601 ctgtagattt ttcttgagaa tataagttca tcatcgcagg caatgtcgac gatttgaatc
      661 aaagcgttgg cctggctcaa acgatgagtg gatgctttcc cctttgcaat caggaattcc
      721 ctccaccgac tgtcgagatt atgaaaattc ggtaaaatcg tatgaagctg ctcaaccgga
      781 aaatccttga gcaaataatg gaagtctccc agtactttac cggcttcaaa agccattgag
      841 ggagataatg ccatatcgta agtcttggaa ttgggcactc tttccattac cctccagtat
      901 tggccttgat catcgtgata gaaggatttt ccttccaaag tttgaattat ccgaacagtc
      961 aaatcttcgg gtgaggaatt tttcgaaata tatcgagtta ctttgaaaat attatccata
     1021 agggcagcca catcagggaa aaccttatgg ttgatgcatt gaaatatgaa gcattgatta
     1081 tccgtatcca cacgaaaagt ctggtggata tggcccgaac caaaaggccg aaccatcgag
     1141 cagcttttat cgaggccaaa agcaacgaat acctcgccgg gtatctttat taatcccata
     1201 gaggctctgg ataatacccc tcacttacca gttttcggag attttcgacg acttttgagc
     1261 gatcttcctg ataagtgata ccaaaccact gttcgttaac cgtgagtact ttgaatcgag
     1321 ccttcccggt cgagataaga tgatcggcta ctgagggaat ataaaattcc gcacttgttg
     1381 aatgatggtt ttcctttaag aattcgagga aaagcctttc gaaatcttga aatatctccg
     1441 ggcagaaagc ccatgtattc atcgaaacaa tggtatcgcc cggtaaaaac actaaatcac
     1501 ctcccgaatt ggggtaggca taacccccat gtgttttatg aatttggtga gtttcaataa
     1561 tttttttcag atagccattg gaatctatct ggcaaattcc tctggaaaca ccgccataat
     1621 ccgataatgt tcttga
//
LOCUS       DAORPD010000439         1632 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_14478_length_1632_cov_2.856056, whole genome shotgun sequence.
ACCESSION   DAORPD010000439 DAORPD010000000
VERSION     DAORPD010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14478_length_1632_cov_2.856056"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1380
                     /locus_tag="PK976_05695"
     CDS             <1..1380
                     /locus_tag="PK976_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRC67413.1"
                     /translation="IIIGTHRILSKDVKFKDLGLVIIDEEQKFGVAAKEKLKALRINI
                     DTLTLTATPIPRTLQFSLLGARDLSVINTPPPNRQPVQTEIRSFSEETIRDAIYYELR
                     RGGQVFFVHNRIQNIMEVAGMLSRFVPDARIAVAHGQMEGHKLEEIMLDFIEGDYDVL
                     VSTTIIESGLDIPNVNTIIINDAQHFGLSELHQLRGRVGRSNKKAFCYLLAPPLASLS
                     DDARRRLRAIEEFSDLGSGFNIAMRDLDIRGAGNLLGAEQSGFIADLGYETYQKILDE
                     AMQELRKTEYSDLFPSEESNTYVNECTFETDLEMRIPVEYIGNITERLALYKELDGME
                     NEDDLQEFALRLEDRFGPLPAPALDLLDSIRLRKMGKSLGMEKIVLKNGQMVCYFITD
                     SNSPFYKSNDFTHVLQYIQTHQRICQLKENKNKLLLTIHHVDMVQKAIQILSEFHEKE
                     KNEIREALK"
BASE COUNT          534 a          329 c          316 g          453 t
ORIGIN      
        1 atcatcattg gcactcatcg cattttgagc aaagacgtca agtttaaaga tttgggtctg
       61 gtaattatag atgaagaaca aaaatttggt gtagccgcta aagaaaaatt aaaagcctta
      121 cgaataaaca tcgatactct cacactcaca gctacaccta ttcctcgtac actccagttt
      181 tccctattgg gagccagaga tttatcggta atcaatactc ctccacctaa tcgccaacct
      241 gttcaaaccg aaatccgttc gtttagtgag gaaactattc gtgatgctat ttattacgaa
      301 ctaaggcgag gaggacaggt attttttgtt cataatcgca ttcaaaatat tatggaagtt
      361 gcaggtatgc tttcacgatt cgttcccgat gcaagaattg cagttgctca tggacagatg
      421 gaaggtcaca aactggaaga gatcatgctc gacttcatag aaggagatta tgatgtcttg
      481 gtgtctacaa cgattatcga atcggggctg gatattccta atgtgaatac gattattatc
      541 aatgatgctc agcattttgg cttgagtgaa ttgcaccagt taagaggtcg tgtaggacgc
      601 agcaataaga aggcattttg ttatctattg gcaccgcctt tggcttcact tagcgatgac
      661 gcccgtagac gtctcagggc tatagaggaa ttttccgatt taggcagtgg tttcaatatt
      721 gccatgcgcg atctcgatat ccgaggagca ggtaaccttc tcggagccga acaaagcggg
      781 tttatagccg atctgggtta tgaaacctat caaaagatcc tcgacgaagc catgcaagaa
      841 cttagaaaaa ctgaatactc cgatctcttc ccatccgaag aatcaaatac ttatgtaaat
      901 gaatgcacct tcgaaaccga tctggaaatg cgcatccccg tcgaatatat cggcaatatc
      961 accgaacgcc tcgcacttta taaagaactc gatggaatgg aaaacgaaga cgacctacag
     1021 gaatttgcac ttcgtctcga agaccgcttc ggcccactgc ctgctccagc cctcgacctt
     1081 ctcgatagta ttcgcctgag aaaaatgggg aaaagcctcg gcatggaaaa aatcgttctc
     1141 aaaaatggac aaatggtttg ttatttcatt accgactcca attcgccatt ttataaatcg
     1201 aacgacttta cccatgtgct tcagtacatt caaactcacc aacgaatttg tcaattgaaa
     1261 gaaaataaga ataaactcct cctcaccatt catcatgtag atatggttca aaaagccata
     1321 cagattctct ctgaatttca tgaaaaagaa aaaaatgaaa ttagagaggc attgaaatag
     1381 atcatcataa gatgcatgtt atttcgaaaa ataaattgtt agcggtaaaa gctaaaaata
     1441 ttttatttat ggaggaaaga tttttcaagg tcagaaaaat atgatttcac aaagttagat
     1501 agaaaaaacg ccctatgttt ctcaatgtaa aaaaatatta attaatagac gtacactgag
     1561 tttcataaag agaaagtctc gtggctcttt gtgaatgatt ttaaccccta aaatcccaat
     1621 ttatacgtaa tt
//
LOCUS       DAORPD010000440         1632 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_14484_length_1632_cov_2.064680, whole genome shotgun sequence.
ACCESSION   DAORPD010000440 DAORPD010000000
VERSION     DAORPD010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14484_length_1632_cov_2.064680"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="PK976_05700"
     CDS             <1..558
                     /locus_tag="PK976_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67414.1"
                     /translation="LFFYVIQLNAQKNNELGIHFGLMANSLVSNANLDGSGSFDGKGA
                     FVLGVKYVRNISNHFSFETGIGYSENKIKIIPAPGLGLVPSEKTIQLVSLPILGNFSF
                     SRNFYLNGGILMDLEIYHSNIQSTHKQSGIGVYLGVGGQYNIERFTLKLIPFVQRHAI
                     IPFEHEKYQQHLTEVGITLVLGYQF"
     gene            complement(606..>1632)
                     /locus_tag="PK976_05705"
     CDS             complement(606..>1632)
                     /locus_tag="PK976_05705"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HRC67415.1"
                     /translation="FEFLIIFRSVTGSLNNTKVEPKKMKKFGLLIFVILIGISFKAVS
                     EDNADSTRSWKLNGNSTLTFNQLHFSNWAEGGESSLSGSGWLNVKVGHYWKKLNSERN
                     LILGLGVLRTQERDLRKTEDKIDYTSIFGYKAFKNWNYTSLINFKSQFTNGYRYPDDS
                     TLISTFMAPGYLTLSLGMQYKPSDNFTIFISPASGRFTFVLNEQLANKGSFGVKPAVY
                     SNDSLHTLLTTGKKIKPEFGINVNAQLKREIFTNVSIESRLILYNNYLDEDIHNRWNI
                     DVNWDALINFHINKFLSSSLRLNLLYDHNIKIKQYAEVDGHQVVIGEGPRLQFKESFG
                     IGLNYKF"
BASE COUNT          509 a          277 c          288 g          558 t
ORIGIN      
        1 ttgttttttt atgttattca gcttaatgct caaaaaaaca atgaattagg cattcatttt
       61 ggtttaatgg ccaacagttt ggtatcgaat gcaaatttag atggaagtgg tagtttcgat
      121 ggtaaaggtg catttgtgct cggggttaaa tatgtgagaa atatttcgaa tcacttttct
      181 tttgaaacag gtattggcta ttctgaaaat aaaattaaaa tcatacctgc acctggttta
      241 ggtcttgttc catcagagaa gacaattcaa ttggtttcat tgccaatttt gggaaatttc
      301 agcttttcaa gaaattttta ccttaatggt ggaattttaa tggatttaga aatatatcat
      361 tccaacattc aatctacaca taaacagtct ggaatagggg tttacctggg agtaggtggt
      421 caatataata tagaaagatt tacattaaag ctgattccat ttgttcagcg acacgccatc
      481 attccattcg aacatgaaaa atatcagcaa catctcaccg aagttggaat tacgctcgtc
      541 cttggatatc aattttaatg aatggaacga aagaattttt tagctgaatt tctattcgga
      601 aaatcttaga atttataatt caatccaatg ccgaaacttt ctttgaattg caggcgtggt
      661 ccttctccta ttactacttg gtgaccatcc acttcggcat attgttttat tttgatatta
      721 tgatcgtaaa gcaagttaag ccttaaactt gagcttaaaa atttattgat atgaaaatta
      781 ataagtgcat cccaattaac atcgatattc catctgttat gaatgtcctc atcgagataa
      841 ttattataaa gaattagccg gctttcgatc gaaacattcg taaaaatttc acgtttgagt
      901 tgggcgttta catttatgcc aaattcaggt ttgatttttt tacctgttgt taataaagta
      961 tgaagggaat cgttggaata gacagcaggt tttactccaa atgaaccttt attggcgagt
     1021 tgttcattga gcacgaaagt aaatcggcca gatgctggag aaataaagat ggtgaaatta
     1081 tcggatggtt tgtactgcat tcccaaagat agggtaagat aaccaggtgc catgaaagta
     1141 gaaatcaaag tcgaatcgtc gggataacga tatccattgg taaactggct cttgaaattt
     1201 attaaagagg tgtaattcca attcttgaat gctttgtatc cgaagatgct agtatagtct
     1261 atcttatctt cggtttttct gaggtctctt tcttgtgtac gaagcacacc caatcccaat
     1321 attaaatttc gctccgaatt taattttttc caataatgtc caacttttac attgagccat
     1381 cccgaaccgc tcaaactgct ttcacctcct tcagcccaat tactgaaatg caattgatta
     1441 aaggtgagtg tgctgttgcc atttaatttc cacgaccttg tactgtcggc gttatcttct
     1501 gacactgcct taaaagaaat acctatcaag ataacaaaaa ttaacaatcc aaattttttc
     1561 attttttttg gttctacctt tgtattgttc aggctaccag ttactgaacg aaaaattatc
     1621 aaaaactcaa ac
//
LOCUS       DAORPD010000441         1630 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_14506_length_1630_cov_2.301587, whole genome shotgun sequence.
ACCESSION   DAORPD010000441 DAORPD010000000
VERSION     DAORPD010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14506_length_1630_cov_2.301587"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1610)
                     /locus_tag="PK976_05710"
     CDS             complement(<1..1610)
                     /locus_tag="PK976_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67416.1"
                     /translation="MSPQIFSFEDFIFEVTPFIQAEPFEKLITLYKAATNNPVLQSRD
                     FDGFLDWGQNVLRDFDEVDAYLIDPARLFPYLRNVKQLATWNLGETPPTEFQNNYLRF
                     YDSMFSLYMEYHRLSMKAKKVSTAMAARWLAENSNFAELFKKWDHIVFAGFYANTPAE
                     EKIISALKDSGKAEIFYDADLYYIKNKLQEAGLFLRDKIDPEKDKWILNRLRDENREI
                     KIIGVAGNVSQTTALQKELEEKLKNEGTAWLDHAAIVLANEDLLIPVLDALPLEIEEV
                     NVTMGLPLIATPVKQLVENFLNIFIQKKDDALPARLFYRLIHHPYIWAEMIDHFHEVN
                     ALRKKIMAGQAYLSHQQLNDVFKDNDTANQLVSLFFEVNNSIDIGNSLLALIDLVKDK
                     FEKGLPQQYLYVFSHILYGTLNKLKESDIDTNPQSFLKLMKRILASTKVPFYGEPLKG
                     LQIMGMLETRALDFDTVFLLSANDDILPGSNRGNSFIPFDVRIEKEFNLPVDAHQSAI
                     SAYHFYRLLQGCKQAFMFYNSEPGGLGGG"
BASE COUNT          503 a          329 c          308 g          490 t
ORIGIN      
        1 cctccgccca aacccccggg ttcgctgttg taaaacataa aggcttgctt gcagccctga
       61 agcaaacggt aaaaatgata ggccgaaata gccgattgat gagcatcgac aggcagattg
      121 aattcttttt ctattcttac atcgaaagga atgaaggaat taccgcggtt gctaccaggc
      181 agaatgtcat cgttggccga caacaaaaaa accgtatcga aatcgagtgc acgggtttcg
      241 agcatgccca taatctgtaa tcccttcaat ggttcaccgt aaaaaggaac tttggtggaa
      301 gccaaaatcc ttttcattag cttcaaaaaa gattgggggt tggtatcgat gtccgattct
      361 tttaatttgt tcaatgttcc atataaaata tggctgaaaa catagagata ttgttgagga
      421 aggcccttct caaatttatc cttaacgagg tcaatcaagg ctaagagcga atttcctata
      481 tcgattgaat tattcacctc aaaaaataag gaaacgagct gatttgcagt atcattatcc
      541 ttgaacacat cattcagttg ctgatgtgat aaataggctt ggccagccat gatttttttt
      601 cttaaagcat ttacttcatg aaaatgatcg atcatttcag cccaaatgta tggatgatgt
      661 ataaggcgat aaaatagcct ggctggcaag gcatcatctt tcttctggat gaagatgttt
      721 aaaaaattct cgacaagttg ttttacaggc gtggctataa gtggtaaacc cattgtaacg
      781 ttcacctcct caatttcaag aggtaaagca tcgagcacag gaatcaacaa atcttcattg
      841 gccaatacaa tagcggcatg atcgagccaa gcagttccct cattcttgag tttttcttcc
      901 aattcttttt gtaatgccgt cgtctgactc acattgcctg caacgccaat aatttttatt
      961 tccctgtttt catctcgtaa tctattgaga atccatttat ccttttctgg atcgatttta
     1021 tctctaagga aaaggccagc ttcttgaagt ttatttttaa tgtaatacaa gtcggcgtcg
     1081 taaaaaattt cggctttacc tgaatccttt aaagcagaaa taattttttc ttctgccggt
     1141 gtatttgcat aaaatccagc aaacacaata tgatcccatt tcttaaaaag ttcggcaaaa
     1201 tttgaattct cggctaacca tcgagcagcc atagctgttg aaaccttttt cgccttcatc
     1261 gacaggcgat gatattccat atagagacta aacatggaat catagaaacg cagatagtta
     1321 ttttgaaatt ctgtaggagg agtttctccc aaattccatg ttgccaattg ctttacattc
     1381 cgcagatagg ggaataatct ggctggatca atcaaatagg catccacttc atcaaaatct
     1441 ctcaaaacat tctgacccca gtcgagaaaa ccgtcgaaat ctcttgattg aagcaccgga
     1501 ttatttgttg cagccttata cagggtgatt aacttctcga aaggttcagc ctgaataaaa
     1561 ggagtaacct cgaagatgaa atcttcgaaa gaaaaaattt gaggagacaa aacaggtcta
     1621 tctatttttg
//
LOCUS       DAORPD010000442         1624 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_14591_length_1624_cov_2.752071, whole genome shotgun sequence.
ACCESSION   DAORPD010000442 DAORPD010000000
VERSION     DAORPD010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14591_length_1624_cov_2.752071"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            202..783
                     /locus_tag="PK976_05715"
     CDS             202..783
                     /locus_tag="PK976_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005640152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HRC67417.1"
                     /translation="MKKVQMEIAGMSFSQVQSGAYALILGEIGGKRRLPIIIGSAEAQ
                     AIAIELEKMKPARPLTHDLFKTFAETFGVRVIEVFINKFIEGVFHAVLVCDDGHNIVE
                     IDSRTSDAVALALRFNCPIYTTEEILDAAGIVIDENALIEEEHSHEAGEEENLLGWEA
                     YSDEELEQMMQEAIENEDYERASKIRDELKRRK"
     gene            811..>1624
                     /locus_tag="PK976_05720"
     CDS             811..>1624
                     /locus_tag="PK976_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+ dependent nucleoside transporter N-terminal
                     domain-containing protein"
                     /protein_id="HRC67418.1"
                     /translation="MKNPTIKLILATLSICFLPIIGMPQVINTPDTLNVQLSLADTAL
                     QGANSVITHAPEELKLGLSLTSVLRGLLGMVVLLAISYFLSMNRKAIEWKVVLTGLAI
                     QIFLAIGVLYIPAIRIIFEVGGKIFVEILNFTKAGSEFLLGKLLDANSFGFIFAFQVL
                     PTIIFFSALMSLLFYLGIVQIVVQGLAWLLAKALKISGAESLCAAGNIFLGQTEAPLM
                     IKPYLPSMSRSEIFLVMTAGMATMAGGVLAAYISFLGGDDPVQRLVFAKHLLS"
BASE COUNT          478 a          274 c          365 g          507 t
ORIGIN      
        1 gtatataaaa aatgtttagg aacttttaga attttgtcgg ttaatagtga aaaaaatata
       61 tataaccaat ttttcgtaag atttgcttta ttttgtatgc cagatataaa ttgatgagag
      121 catgaagttt ttaagggaat atacatatta aaatattata ttaatcgact gatttacaat
      181 aataaatata gtaaattaag gatgaagaaa gttcaaatgg agattgccgg aatgtcattt
      241 agccaagtac aaagtggtgc gtatgctttg attttaggtg aaattggagg caaaaggcgt
      301 ttacccatca ttataggaag tgccgaggct caagctatag ctatcgaact ggaaaaaatg
      361 aaacccgcca ggccattaac ccatgactta ttcaagactt ttgccgaaac ttttggcgta
      421 cgggtgatcg aggtgtttat caataaattt atcgaaggag tttttcatgc tgtcttggta
      481 tgcgacgacg ggcataatat tgttgaaatt gattcgagga cttccgatgc cgttgctttg
      541 gctctgcgat tcaattgccc tatctatact accgaagaaa ttctcgatgc cgcaggaata
      601 gtgatcgatg aaaatgcact gattgaagaa gagcactcac atgaagcagg tgaagaagaa
      661 aatttattgg gatgggaggc atattccgat gaagagttag aacaaatgat gcaggaagcc
      721 attgaaaatg aagattatga aagagcaagt aagattaggg atgaactgaa acgaagaaaa
      781 taagactaac ctaattgtca tataattcct atgaaaaatc ctaccatcaa actgattctc
      841 gctacattat cgatttgttt tttaccaatt ataggaatgc cgcaggtaat aaatacgcct
      901 gataccctga atgtacagct atctttagcc gatacagcct tgcaaggagc aaactcagta
      961 atcactcatg ctcctgagga actcaaactg ggtctttcac taacctccgt tctgagaggg
     1021 ctcctgggca tggttgtttt attggctata tcgtattttt tgagcatgaa tcggaaagcc
     1081 atcgaatgga aagtggttct taccggattg gccatacaga tttttttagc tattggtgtt
     1141 ttatatattc ctgctatacg tattatattc gaagtgggtg gtaagatatt tgtcgagatt
     1201 ttgaatttta ccaaagcagg gagtgaattt cttttaggga agctgctcga tgcaaattcc
     1261 ttcggtttta tttttgcttt tcaagtttta ccgaccatta ttttcttttc ggctttaatg
     1321 agccttttgt tttatttagg aattgtgcag atagtggtgc aagggcttgc atggttattg
     1381 gccaaagcct tgaagatttc gggtgccgag agcctgtgtg ctgctggcaa catatttctg
     1441 ggccaaaccg aagctcccct catgattaaa ccctatcttc cctcaatgag ccgttcggaa
     1501 atatttctgg tcatgacggc tgggatggct acgatggcgg gaggagtgct ggctgcttac
     1561 attagctttt taggaggcga tgacccggtc cagaggctcg tcttcgctaa acatctactt
     1621 tcgg
//
LOCUS       DAORPD010000443         1621 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_14632_length_1621_cov_2.392082, whole genome shotgun sequence.
ACCESSION   DAORPD010000443 DAORPD010000000
VERSION     DAORPD010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14632_length_1621_cov_2.392082"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(154..>1621)
                     /locus_tag="PK976_05725"
     CDS             complement(154..>1621)
                     /locus_tag="PK976_05725"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC67419.1"
                     /translation="GVNYTTQSGMTNYVWAVSSGGNITGGQGTNAITVNWNGSGAQTV
                     SVNYINASGCTASSPSVLNVTVNPTPTPSINGPANPCLSPNYYDYFTEAGMSNYQWTV
                     SSGGSIYSGQGTANLRVIWNTTGLQSISVSYVSPQGCMMPGPYSKTIFVQPLPNAAGA
                     ITGSGEVCAGESGVVYSVGNILNATSYQWTVPGGATIVSGQGSNTITVNFASNAISGE
                     VSVAGMNGCGLGVPSFLAVIVKPLPGVAGTIAGHPNVCQGATNVTYSVAPIANASGYV
                     WSLPVGASITAGANTNSIKVSFSENAVSGNISVYGTNACGNGISSPEFFVTVNPIPPT
                     PTITVSNYTLTSSAPEGNQWYLNGVPIPDATGQSYTATETGNYYVIVTLNGCSSAPSN
                     TILVIIEGMISLDHNSFKVYPIPNDGRFTIELVLQKSGIYTLEIFNSLGLRVYSSEIY
                     VDGKHILPMDLSYLPSGVYTVTLFNTQLNAVRRIVISK"
BASE COUNT          499 a          400 c          283 g          439 t
ORIGIN      
        1 acaagcaaac cacgataaca caatatttac aagaaacggt ttacttttta tttctttgtc
       61 atgttagcta ttcattaaaa cggccggata aaaaaactct ggcctgcggc agagcacaag
      121 ccagagtgta taggacttta gagcaaatct gccctatttg ctgatcacga ttcttctcac
      181 tgcattcaac tgcgtattga acaaagttac cgtataaaca cccgaaggca gataactcaa
      241 atccatcggc aaaatatgtt tcccatcgac ataaatctca ctgctataaa ccctcaatcc
      301 taaactattg aagatttcta aagtatagat tcctgatttc tgcaacacta actcgatggt
      361 aaatctccca tcattgggaa taggataaac cttaaatgaa ttatgatcga gcgaaatcat
      421 gccttctata attaccaaaa tggtattgga aggtgctgaa ctacaaccat tcaaggttac
      481 aattacatag taattgcccg tttccgttgc agtatagctt tgtccagtag cgtccggaat
      541 gggaactcca ttcaaatacc attgattgcc ttccggtgca ctgcttgtta aagtataatt
      601 cgacaccgta atggtaggag taggaggaat gggattcaca gtaacaaaga attcgggtga
      661 agaaatacca ttgccgcaag catttgtccc atataccgaa atattaccag aaacagcgtt
      721 ttcactaaaa cttaccttga tggaattggt atttgcccct gccgttatgc ttgcacccac
      781 cggcaaactc catacataac ccgaagcatt agctatagga gcaactgaat aggtaacatt
      841 tgtggcaccc tggcatacat tcggatgccc agcaattgta ccggctactc ctggtaaagg
      901 tttcacaatt acagccaaaa aagaaggcac acctaaacca caaccattca tccctgctac
      961 acttacttcg ccagaaatgg cattcgaagc aaaatttacc gtaatagtgt tgcttccctg
     1021 accagaaact atggttgctc ctcctggaac agtccattga tatgaagtag cattgagaat
     1081 attccctact gaataaacta ctccgctttc cccagcacaa acctcacctg aaccagttat
     1141 tgctcctgcc gcattaggca aaggctgtac aaatatcgtc ttactatatg gccctggcat
     1201 catgcacccc tgtggactta catacgaaac actgatggat tgtaatcccg tcgtattcca
     1261 tattactctt aaattcgccg tcccctgacc actatatatc gacccacctg aagaaacagt
     1321 ccactgataa ttgctcatac ctgcctcagt aaaataatca taataattcg gactcaaaca
     1381 gggattagcc ggtccattga tagaaggcgt cggagtagga tttaccgtaa cattcaaaac
     1441 actcggactc gatgccgtac atcccgaggc atttatgtaa ttcacactta cagtttgtgc
     1501 accactacca ttccaattca ctgttatagc attcgtacct tgacctccag taatattgcc
     1561 acctgacgat actgcccaaa cataattggt catccccgac tgtgtggtgt aatttactcc
     1621 t
//
LOCUS       DAORPD010000444         1621 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_14637_length_1621_cov_1.958493, whole genome shotgun sequence.
ACCESSION   DAORPD010000444 DAORPD010000000
VERSION     DAORPD010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14637_length_1621_cov_1.958493"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            116..823
                     /locus_tag="PK976_05730"
     CDS             116..823
                     /locus_tag="PK976_05730"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRC67420.1"
                     /translation="MKKNLSTLFLLLCILLLPILSMSQKFGLKAGYNFSSLGYDGLGK
                     DIYSNTSDIRTSTTFGGYINCKLIPLVSFQIECNYEPKGIKYGTPPLPYINASFDGIY
                     NEHFDYLCIPLLLRINLIAFQIEAGPYFGFLLKAEETKKGFQKTPGAQGEWIEEHFDE
                     VYDLKSNTKETDMGLTAGMGVSYSIGPMQILGGARYNYGFTNILKEPILNDKEEVWID
                     KKNNRNFNLYIGLGLAL"
     gene            complement(1020..>1621)
                     /locus_tag="PK976_05735"
     CDS             complement(1020..>1621)
                     /locus_tag="PK976_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67421.1"
                     /translation="RNIQARTEAGTERWSFDITDKNNQSNSVALNITTQPVVNIYTAI
                     LMGGQLNVTIGSFWSSVDNKVMKQAEAVLNQNKVDFLYFYGETNKATIAATDDDQAAI
                     AWNNIFDNWTVKNATRFKKVSGINWNAVTEYDDALIAENATNLVDSRANLLEVGDIVA
                     FQTATTSANPGKKGLFKVMEIAGTVGNDRTITLEVKIQK"
BASE COUNT          496 a          282 c          305 g          538 t
ORIGIN      
        1 acttaaaccg gctttagcaa taaatctttt cgaacaactg actcgtattt ttcagaatta
       61 agagagattt tcgtaatctt gcaaagtagc cgaatctatc gaaatttatt ttcgtatgaa
      121 aaaaaatctt tcgaccttat ttttgctttt atgcatatta ttactaccta ttttgagcat
      181 gtctcaaaaa tttgggctaa aagctggata taatttttcg agtcttggct atgatggatt
      241 gggtaaggat atttattcga atacaagtga tataaggact tctacgactt ttggaggata
      301 tatcaactgc aaactcattc cattggtttc ttttcagata gaatgtaact acgaacctaa
      361 agggataaag tatggaactc ctcccctgcc atatatcaat gccagttttg atggaattta
      421 caacgaacat tttgattatc tttgcattcc tttactttta cgaatcaacc ttattgcctt
      481 tcaaattgaa gccgggcctt attttggctt tttattaaag gcagaagaaa caaagaaggg
      541 ctttcaaaag acacccggtg ctcaaggcga atggatcgaa gagcatttcg acgaagtata
      601 tgatttaaag tccaatacaa aagaaactga tatgggtcta actgctggga tgggggtttc
      661 ttattccatt ggtcctatgc agattttagg aggagcaaga tacaattatg gttttactaa
      721 tatactgaaa gaacccattt tgaacgataa ggaagaagtt tggatcgaca agaagaataa
      781 ccgcaacttt aatctctaca taggattagg cttagctctc tagaaaaatt ctaactcatt
      841 agattaaata aaaataaata taaaggtttc cccgaagaaa tctaatatca aaagtttcta
      901 tgattttccc agggtatgaa ttgggataaa ataatacaag ttgtttatgt ggttatagaa
      961 aagggaggct cgatggaacc tcctctttgt ttttacagat tctaaagatg tattaaggat
     1021 tatttttgaa ttttaacctc gagggttata gtccggtcgt tgccaacagt accagcaatc
     1081 tccattactt tgaataatcc ctttttaccc ggattggctg aagtagtggc tgtttggaaa
     1141 gctacgatat cgcccacctc aaggagattt gctcttgaat cgacaagatt ggtagcattt
     1201 tcagcaatta atgcatcatc atattcggtt actgcattcc agttaatacc actaaccttt
     1261 ttaaaacgtg tggcattttt tacggtccaa ttatcgaata tattattcca ggcaatagca
     1321 gcctgatcgt cgtcggtagc ggctatagtc gccttgttgg tttctccgta aaagtagaga
     1381 aaatctactt tattctgatt taaaacagct tcggcttgtt tcattacttt attatcgact
     1441 gaactccaaa agcttccaat ggtaacattg agttgtccac ccatcaaaat agcggtataa
     1501 atattaacta ctggttgtgt tgtaatattc agagctactg aattactctg attgttttta
     1561 tcggtaatgt cgaaggacca gcgttcggtg ccggcttcgg tacgagcctg aatatttctt
     1621 g
//
LOCUS       DAORPD010000445         1616 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_14703_length_1616_cov_1.701473, whole genome shotgun sequence.
ACCESSION   DAORPD010000445 DAORPD010000000
VERSION     DAORPD010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14703_length_1616_cov_1.701473"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="PK976_05740"
     CDS             <1..516
                     /locus_tag="PK976_05740"
                     /inference="COORDINATES: protein motif:HMM:NF027879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MIT C-terminal domain-containing protein"
                     /protein_id="HRC67422.1"
                     /translation="ATDESSDAKDKHDELSAKTIHIREGETGYSYNNLFGPYLKGATK
                     IFVVDPYVRLEYQIRNFIVFAGIIDTSVGQVELKLTTSAEDAYQEKIQEQKLKEISAS
                     LAQHGIIFTFDFDPVIHDRSIRMDNGWGIYPGRGLDIYQKPDSKYELSEIDQTKRKCR
                     ETDIIFQQAKG"
     gene            521..>1616
                     /locus_tag="PK976_05745"
     CDS             521..>1616
                     /locus_tag="PK976_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67423.1"
                     /translation="MATWVEDIVQALKNLGGQAHRKQILEEVKRIRKEPLPERIAEVI
                     QRTIQDHSSDSTGFRGNDLFRKVGNGVWALRGQGDVETLSQAPQSFSSNIVKNEVTTT
                     WVEDIVRAITNLGGQAPLTQIYEEVKRIRQGPLPKMWQHIIQDTIYQHSSDTQKFQGN
                     DIFRKVDKGVWSLRNHVETSQPVLRALKQKATVPQNYLPPESFEEIANILRTIKQYRD
                     YQHPDSSTWKEYVDEFFHVLGFSTNEKNQRIMTLNLIGANHTPKAIVCYVKPGENFDE
                     ITPGLEWESYLFYAAKFHQVEWGILTDGLQLKVIQFIDQDKKEPSYWPDLDGIVCKEK
                     LDTFCTVYKVFSFIKGYNGKPTVQGHQKKSQ"
BASE COUNT          536 a          305 c          341 g          434 t
ORIGIN      
        1 gctactgatg aatcctcaga tgccaaagat aaacatgatg aactttctgc gaagactatc
       61 catattcgag aaggtgaaac tggttatagc tacaataatc tgttcggacc ttatctcaag
      121 ggcgcaacga aaatttttgt ggttgaccct tacgtccgtt tagaatatca gatcagaaat
      181 tttatcgttt ttgccgggat tattgatact tcagttggtc aggttgaatt aaagctcacc
      241 acttctgcag aagatgctta tcaagagaaa atccaagaac aaaagctcaa agagatcagc
      301 gccagccttg cacaacatgg aattattttt acctttgact tcgatccagt tatacatgat
      361 agaagcatac ggatggataa tggctggggt atttatccag ggcgcggttt agatatttat
      421 caaaagcctg attcgaagta cgagttgagc gagatcgatc agacaaaacg aaaatgtcgg
      481 gagacagata ttatcttcca acaagcaaag gggtgagtac atggcaacat gggtcgaaga
      541 tattgttcag gctttgaaaa acctcggtgg tcaagcccat aggaaacaga ttttagaaga
      601 agtaaaacgg attaggaaag agcctttacc agaaagaatt gcagaggtaa ttcaaaggac
      661 tattcaagat cattcttctg attcaaccgg atttagagga aatgatttgt tccgaaaagt
      721 tggaaatggt gtttgggcgc ttaggggcca aggcgacgta gaaactttga gccaggcacc
      781 tcaatctttt tcatccaaca tagtaaaaaa cgaagtaaca actacttggg ttgaagatat
      841 tgttagggct attacgaatt tgggtggtca ggctcccctt acccagatct acgaagaggt
      901 aaagcgtatt cggcaaggtc cgcttccgaa aatgtggcaa cacataattc aagacacgat
      961 ttatcaacac tcttctgata ctcaaaaatt tcaggggaat gacatatttc gaaaagtaga
     1021 taaaggagtt tggtcgcttc gaaaccatgt ggaaacctcg caacccgttc ttcgcgccct
     1081 aaaacaaaaa gctactgttc ctcaaaacta tcttcctcct gagtctttcg aggaaattgc
     1141 aaatattcta cggacgataa aacaatatcg agattaccaa catccagatt cgtcaacctg
     1201 gaaagaatat gtggatgagt ttttccatgt cctaggtttt tctacgaatg aaaaaaacca
     1261 aagaatcatg actttaaatc tcattggagc aaaccacacc cctaaagcaa tcgtttgtta
     1321 cgtaaagcca ggtgaaaact tcgatgaaat taccccaggg ttagaatggg aatcctatct
     1381 gttttatgct gccaaatttc atcaggttga atggggaata ttaacagatg gtctccagct
     1441 taaggttatt caatttatag atcaagacaa aaaagagcca tcctattggc cagatcttga
     1501 tggaattgtc tgtaaagaaa aacttgatac attttgcacg gtttacaagg tgttttcttt
     1561 tatcaaagga tataacggca agccaactgt gcaagggcat caaaaaaagt cacaac
//
LOCUS       DAORPD010000446         1615 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_14714_length_1615_cov_2.428846, whole genome shotgun sequence.
ACCESSION   DAORPD010000446 DAORPD010000000
VERSION     DAORPD010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14714_length_1615_cov_2.428846"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1608)
                     /locus_tag="PK976_05750"
     CDS             complement(<1..1608)
                     /locus_tag="PK976_05750"
                     /inference="COORDINATES: protein motif:HMM:NF012688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HRC67424.1"
                     /translation="MKKILDFLEFRAFSQRIFKDLNHDKPSEQLSFFPEETSSRHESD
                     TNLFKNIHTTPHQYLTLTSLEEIRSFADTLQKSSEFCFDTETTGIDPNNCDLVGMSFA
                     VVAHRAWYIPMPVQRDEILDILQILKPVFHNRKILKIGQNLKFDLIVLQWYGCNIYGP
                     FFDTMLAHYVIEPEQRHSMDYLARAYLNYDPVPIESLIGKKGNNQRNMQAVELETIKE
                     YAGEDADITLQLKHAIEPLLHQTSTYELFQEIEMPLMPVLVSMEIEGVKIDTEALDAY
                     GKQLENEIHRLEDEIYSLAGESFNISSPKQLGIILFEKLKISENPRLTSTKQYATGED
                     VLEKLAHRHPIVEKILDYRELTKLKSTYVDTLPQMINPRTQRVHTSYNQAVTATGRLS
                     SNNPNLQNIPVRTERGREIRRAFIPRNPDYILISADYSQIELRIIAHLSGDEAMKEAF
                     RQGLDIHAATASRIFNIPLDQVTKEQRRTAKSVNFGIVYGISAYGLSQNLDIAKKEAA
                     AIIEEYFRQYPGIKAYMEKSIAFAREHG"
BASE COUNT          437 a          322 c          343 g          513 t
ORIGIN      
        1 accgtgctcc cgtgcaaacg caatactttt ttccatatag gcctttatac cgggatattg
       61 cctaaaatat tcttctataa tagctgcggc ttccttttta gctatgtcga ggttttgtga
      121 aaggccataa gccgaaattc cataaacaat tccaaaattt accgattttg cagtgcgtcg
      181 ttgttctttg gtaacctgat cgagaggaat attaaaaatg cgtgatgccg tagccgcatg
      241 tatatcgagg ccttgcctga aagcttcttt catagcctcg tcgccactca ggtgagcaat
      301 gattctcaat tcgatctgag aataatcggc agaaatcaat atataatcag ggtttctggg
      361 gataaaggcc ctgcggattt cgcggccacg ttctgtccgc acgggaatat tctgcaaatt
      421 tgggttattg ctgctcaatc tccccgtagc agtcaccgcc tgattataac ttgtatgcac
      481 acgctgagtt cggggattga tcatctgggg cagtgtatct acataagtgg atttaagctt
      541 tgttaattct ctataatcga gaatcttttc gacaatagga tgacgatgtg ctaatttttc
      601 gagtacatct tctcctgtgg catattgttt ggtactcgtc agccgaggat tttcagaaat
      661 ttttaatttt tcgaataaga tgattcctaa ttgtttagga gaagaaatat tgaaagattc
      721 acccgccaga gagtatattt catcttcgag cctatgtatc tcattttcga gttgtttccc
      781 ataagcgtcg agggcttcgg tgtcaatttt tacgccttct atttccatgc tcacaagcac
      841 aggcatcaga ggcatttcta tttcctgaaa caactcgtaa gtgcttgtct ggtgtaaaag
      901 aggttcgatg gcatgcttca attgaagggt aatgtcggca tcttcgcctg catattcctt
      961 gattgtttcg agttccaccg cttgcatatt tctctgatta tttcctttct tcccaatgag
     1021 tgattcgata ggaactggat cgtaattgag ataggctcgt gcgagataat ccatgctatg
     1081 tcgttgttcg ggctctatta cataatgtgc cagcatggta tcgaaaaacg gcccgtatat
     1141 gttgcatcca taccattgta gcacaataag atcaaatttc aggttctggc caattttcaa
     1201 aatctttcga ttatggaaaa caggctttaa aatctggaga atatcaagga tttcatcgcg
     1261 ttgtacgggc ataggaatat accatgctcg atgagctacc acggcgaacg acatacccac
     1321 tagatcacaa ttattgggat cgatgccggt agtctctgta tcgaagcaaa attccgaaga
     1381 tttttgcaga gtatcggcaa aagaacggat ttcctctaaa gaagtaaggg tgagatattg
     1441 atgaggagtg gtatgaatat ttttaaacaa attcgtatcg gattcatgcc tgcttgaagt
     1501 ttcttctgga aaaaatgaaa gctgttcact gggtttatca tgattcaaat ccttgaatat
     1561 tcgctgcgaa aaagcacgaa attccagaaa atccaatatc ttcttcaagg tgttt
//
LOCUS       DAORPD010000447         1613 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_14746_length_1613_cov_2.716303, whole genome shotgun sequence.
ACCESSION   DAORPD010000447 DAORPD010000000
VERSION     DAORPD010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14746_length_1613_cov_2.716303"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1021
                     /gene="gldE"
                     /locus_tag="PK976_05755"
     CDS             <1..1021
                     /gene="gldE"
                     /locus_tag="PK976_05755"
                     /inference="COORDINATES: protein motif:HMM:TIGR03520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated protein GldE"
                     /protein_id="HRC67425.1"
                     /translation="YPLATFIIQSVVVTFIILFFGEISPKIYSTHKKESIIRLMATPL
                     FVLEKLFKPINFVLVKSTGIVDRRLKKREDDISLKDISEAINITKTSSDKHDEARMFK
                     GIVNFGNIQVREIMVPRVDAVAVNDNTPYDELLQIIRQSGYSRIPVYSGSPDSVVGIL
                     YIKDLLKHLNEGKDYPWQRHLRPAYYIPENKKIDTLLTEFQSQKIHIAIVVDEYGGTS
                     GIVTLEDILEEIFGDISDEFDEEEDEKNYVKVDENTYIFDGKILLNDFCRILQIREDI
                     FDEVSGEFDTLAGLILELEGRLPATNEVIFYKNFEFRIESADYKRIKGVRVTYHYDEE
                     EDKKK"
     gene            1021..>1613
                     /gene="gldD"
                     /locus_tag="PK976_05760"
     CDS             1021..>1613
                     /gene="gldD"
                     /locus_tag="PK976_05760"
                     /inference="COORDINATES: protein motif:HMM:TIGR03512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldD"
                     /protein_id="HRC67426.1"
                     /translation="MFKRFSLFMFEVLTMAVILTSCHHQPIPKPKGFLRIDLPQKAYK
                     DFDTTFPYAFQYPAYSLIKPARKPVENPYWIDIVFPIFKATVYLSYKPIHDNLQQYTE
                     DSRTFVMKHIPKASSIEEFVVQNPDANVYGLFWLIKGREAASPCQFYLTDSSKHFLRG
                     ALYYDFNPNNDSLAPVTNFLISDIQHLAQTLKWKNIG"
BASE COUNT          516 a          327 c          303 g          467 t
ORIGIN      
        1 ttatccactg gctaccttta ttatacagtc ggtagtagtc actttcataa tactgttttt
       61 cggagaaatc agcccaaaga tatatagtac gcacaagaaa gaatccatta tccgccttat
      121 ggctactcca ttgtttgtgt tagaaaagct attcaaacca attaactttg ttttggtaaa
      181 atctacgggc atagtggatc gaagattgaa gaaacgagaa gatgacattt cactcaaaga
      241 tatttcggag gccattaaca ttacgaaaac ctcatcagat aaacacgacg aagccagaat
      301 gttcaaaggc atcgtaaatt tcggaaacat tcaggtcaga gaaatcatgg ttccccgtgt
      361 ggatgctgta gccgtaaatg ataacactcc ttatgatgaa ttgttacaaa taatccgaca
      421 atcgggatat tcgcgaattc ctgtgtacag tggttcaccc gattcagtag ttggaattct
      481 gtatatcaaa gacctgttga aacatctcaa tgaagggaaa gattatccct ggcagcgaca
      541 tctccgccct gcatattata ttcccgaaaa taaaaaaata gatactctac ttactgaatt
      601 tcaaagtcag aaaattcata ttgctatcgt tgtagatgaa tatggtggaa cttcgggtat
      661 tgtgaccctc gaagacatcc tggaggaaat tttcggagat ataagcgatg aattcgatga
      721 ggaagaagat gaaaaaaact atgtgaaagt agatgaaaat acatacatct ttgatggaaa
      781 aatcttattg aatgattttt gccgtattct tcaaattcgt gaagacattt tcgatgaagt
      841 gagcggagaa ttcgatactc ttgctggatt gatccttgaa ctcgaaggca gactccctgc
      901 aaccaatgag gttattttct acaagaactt cgagtttaga atagaatcgg ccgattacaa
      961 acgcattaaa ggggtcaggg ttacttatca ttacgacgaa gaggaggata aaaaaaaata
     1021 atgtttaaac gtttctctct ttttatgttt gaggtattga ccatggcagt gattttgact
     1081 tcatgccatc atcaacctat tccgaagcca aaaggtttct tgcgcatcga ccttccgcaa
     1141 aaagcttaca aggattttga tactaccttt ccctatgctt ttcaataccc tgcatatagt
     1201 ttaataaaac cagcacgaaa acccgtcgaa aacccttact ggatcgacat cgtcttccct
     1261 atattcaagg caacggttta cctcagttat aaacctatac acgataattt gcaacaatac
     1321 accgaagact ctcgtacttt cgtgatgaaa cacattccca aagcctcttc cattgaagaa
     1381 ttcgtggtgc agaatcccga cgctaacgtt tatgggcttt tctggctcat caagggtcga
     1441 gaagcagctt cgccctgcca gttctatctc accgatagtt caaagcattt cttgagagga
     1501 gctttgtatt atgatttcaa tcccaacaat gattccttag caccggttac caatttcctg
     1561 atttccgata ttcaacattt agctcaaaca ctgaaatgga aaaacattgg cca
//
LOCUS       DAORPD010000448         1610 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_14791_length_1610_cov_2.172990, whole genome shotgun sequence.
ACCESSION   DAORPD010000448 DAORPD010000000
VERSION     DAORPD010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14791_length_1610_cov_2.172990"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="PK976_05765"
     CDS             <1..422
                     /locus_tag="PK976_05765"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HRC67427.1"
                     /translation="IGGKTYFQPELLYSARGGKFTGIPQGETKESQYTYSIDAIDIPF
                     LVGSKIINIPFVKVRIFAGPVASFFLDKEMKKDDNKEDQEQLKDGVWALTLGAGVDIL
                     MFTLDMRYELGLNNISNTPGQSIKNNLFNITLGWKIL"
     gene            528..1532
                     /locus_tag="PK976_05770"
     CDS             528..1532
                     /locus_tag="PK976_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67428.1"
                     /translation="MRKYIIISFAVVSLLAFSGLYCRNKMDKPELNKIEIQKVSIQRY
                     EQDLFRIPIDSLQELLKHLANKYPLFIGNEYDNPASLLQMRQYLNDPQIIEAYVQACK
                     TFPSLAQIEDGLTEAFRYIHYYFPSWHPPKSVYSYISGYDLAEGIFIDDSVLIIPLDN
                     YLGSNFPGYRTIGIPKYIAERMTPDYLIPDIIKTIAQQFLPPFPVDGALVDQMIAYGQ
                     LLFFAQNILPKTPPYFLMGYTAEKYKWCQDNEKEIWSFLLNQDLLFSKDKSIIMKFIQ
                     ESPTIQGFPKGSPGRIGQYMGLRIIESYARKHPDKNLKEIIQEEDYAVLFNQSGYKPR
                     "
BASE COUNT          517 a          295 c          310 g          488 t
ORIGIN      
        1 ggataggagg caagacttat tttcaacccg aacttttata ttctgccaga ggtggaaagt
       61 ttacgggcat tccccaagga gaaacaaaag aatcgcaata tacgtattcg attgatgcca
      121 ttgatattcc atttttggtg ggtagcaaaa tcatcaatat tccttttgta aaggtcagaa
      181 tatttgcggg acctgtggct tcgttcttcc tcgacaagga aatgaagaaa gacgataata
      241 aagaggatca agaacaattg aaggatggcg tttgggctct caccttaggt gcaggggtgg
      301 atatattgat gtttacactc gacatgaggt acgaattagg cttaaataat atctccaata
      361 ctcccggtca atctatcaag aacaatcttt ttaatattac attaggttgg aaaatacttt
      421 aaaagctcac atgaaggttt ataccccatg aagcccagca tgaatcatta ctgggcttct
      481 tttctatatg ctacaaatca tggtcttcta ttatttacat tacacctatg cggaaataca
      541 tcatcatttc atttgcagta gtctcacttt tagctttttc gggattatat tgcagaaata
      601 aaatggataa acccgaactt aataaaattg agattcaaaa agttagcatt caacgatacg
      661 aacaggattt atttcgtatt cctatcgata gcttgcaaga attgctgaag catttggcaa
      721 acaaatatcc cttatttata gggaatgagt atgataatcc ggcaagtttg ctgcaaatgc
      781 ggcaatattt gaatgatcct caaattatag aggcttatgt tcaagcctgt aaaacatttc
      841 cttccttggc gcaaatcgaa gatggtttaa ctgaagcttt tcggtatatc cattattatt
      901 tcccttcatg gcatccaccc aaatcggtat attcttacat cagtggctac gatttagccg
      961 aaggaatatt tatcgatgac tcggttttga ttattccgct cgacaattat ctaggaagta
     1021 attttccggg atatagaacc attggcatac ctaaatacat tgctgagaga atgacgccag
     1081 attatttgat tcccgacatt attaaaacga tagcacagca atttttacct ccttttcctg
     1141 tcgatggagc tctggttgat cagatgatag cctatggaca attattgttt tttgctcaaa
     1201 atattcttcc caaaactcca ccatattttc tcatggggta cactgccgaa aaatataaat
     1261 ggtgtcagga taacgaaaag gaaatctggt cgtttctatt gaaccaggat ctacttttta
     1321 gtaaagataa aagcataatc atgaaattta ttcaagaaag tcctacaatt caaggatttc
     1381 caaaaggaag tcccggtcgt attggtcagt atatgggttt gagaatcatt gaatcttatg
     1441 ccaggaaaca tcccgacaag aatctcaagg aaatcatcca ggaggaagat tatgcggtgc
     1501 tcttcaatca gtcaggatat aaaccacgtt gatagccaca atcaagaatt tggtttgcag
     1561 atcaagtcca agtaagctaa tgacgggaag ggaagtaggg aatccaaacc
//
LOCUS       DAORPD010000449         1604 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_14867_length_1604_cov_2.571982, whole genome shotgun sequence.
ACCESSION   DAORPD010000449 DAORPD010000000
VERSION     DAORPD010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14867_length_1604_cov_2.571982"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1604
                     /locus_tag="PK976_05775"
     CDS             <1..>1604
                     /locus_tag="PK976_05775"
                     /inference="COORDINATES: protein motif:HMM:TIGR04057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HRC67429.1"
                     /translation="DDIESISVLKGPAAAALYGSRAANGVILITTKKGKAGAVKGIGV
                     SINSGISIENVAYLPKYQNLYGGGTTFTGAGTKDGFLVQEINGIEYLLPDYATDESWG
                     PRYNPNIYYLPYWSLYDWEALGKPTTNADGTPYRLETAPWVAPKHDVEDFFETSVGYT
                     NNIALTSASENGSFRLSYTNFSSNGYMPNSTLNRNTFNFNGTSKFGKKLMAFTSFNYI
                     KTYALGRPVTGYDDNNVLQKFTQWGQRQLDMEKMKNYMNPDGTQRTWNRRAWFDPLPN
                     YSDNFYWTRFMNYEEDWRDRYYGNVGLTYQIFDWLKLTGKYNMDTYTFRINERVAIGS
                     QALSYYQEDVRQLTETNAEFLLEADKRFGDDFTTKLTLGGNRMHRQYELNGGITQGGL
                     VIRNFYNLRNCLSPALVHDETSQKSINSLYGSLNLGYKGLLYLDVTLRNDWSSTLPKN
                     NWSYPYPSVTLSYVFSEMPGLQNQNWFDFGKLRFGWAEVGNDTEPYRLITLYENIQNF
                     GSDIIYTLPGMLNNAKLRPESTNGWE"
BASE COUNT          472 a          321 c          338 g          473 t
ORIGIN      
        1 gatgatattg agtctatctc tgtattaaaa ggtccagcag ctgctgctct ttatggctca
       61 cgcgctgcca atggcgttat tcttattact acaaaaaaag ggaaagctgg agctgttaaa
      121 gggattggcg tttctatcaa ttccggcatt tcgatcgaaa atgtagctta tcttcctaaa
      181 taccaaaatc tttatggtgg aggtacaact tttacaggag cagggacaaa ggacggtttc
      241 ttagtacaag aaatcaatgg tattgagtat ttgcttcccg actatgctac tgatgaaagc
      301 tggggcccgc gttacaatcc caacatttat tatttgccct attggagtct ttatgactgg
      361 gaagccttgg gcaagcctac aaccaatgct gatggtacac cctatcgttt agaaaccgct
      421 ccctgggtag ctcctaaaca cgatgttgaa gacttcttcg aaacctcggt aggatatact
      481 aataacattg ctttgaccag cgccagcgaa aatggttcgt tccgcctgtc gtataccaat
      541 ttctcttcca atggatatat gcccaactct acccttaaca ggaatacctt caattttaac
      601 ggaacatcga agtttgggaa gaaacttatg gcattcactt catttaatta tatcaagact
      661 tatgctctgg gaaggcctgt tactggatac gacgataaca atgtactgca gaaatttacc
      721 cagtggggtc agcgtcaact cgatatggaa aaaatgaaaa actatatgaa ccccgatgga
      781 acccaacgta catggaaccg cagagcatgg tttgatccat tgcctaatta ttcggacaat
      841 ttttattgga cacgttttat gaattacgaa gaagactggc gtgatcgtta ttatgggaat
      901 gttggcctta cctatcaaat ttttgattgg ttaaaattga cgggtaaata taatatggac
      961 acttacacat ttcgtatcaa cgagagagta gccataggaa gtcaggcctt atcttactat
     1021 caggaagacg ttagacagct gaccgaaacc aatgctgagt ttttgctgga agccgataaa
     1081 cgttttggtg atgattttac cactaaactt accttaggag ggaatcgcat gcaccgccag
     1141 tacgagctca atggtggtat cacacagggt ggcttggtta tcaggaattt ctataacttg
     1201 agaaattgtc tttctccggc actcgttcat gacgaaacat cacaaaaatc cattaattct
     1261 ctctatggca gcttgaatct gggttataaa ggcctgttat atcttgatgt aaccttacgt
     1321 aatgactggt catctacatt gcctaaaaat aactggtcat atccttatcc ttccgtaacc
     1381 cttagttatg tattcagtga aatgccagga ttgcagaatc aaaactggtt cgattttggc
     1441 aagttgagat tcgggtgggc tgaagtaggt aatgatactg agccttatcg tttaatcacc
     1501 ttgtatgaaa acattcagaa ttttggaagt gatattattt acacacttcc cggcatgctc
     1561 aacaatgcta aattaaggcc tgaaagcacg aatggctggg aaat
//
LOCUS       DAORPD010000450         1603 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_14880_length_1603_cov_1.885659, whole genome shotgun sequence.
ACCESSION   DAORPD010000450 DAORPD010000000
VERSION     DAORPD010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14880_length_1603_cov_1.885659"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            7..969
                     /gene="pta"
                     /locus_tag="PK976_05780"
     CDS             7..969
                     /gene="pta"
                     /locus_tag="PK976_05780"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HRC67430.1"
                     /translation="MNKCIVLPEGEEERTLIAANEVLKENLARIILMGYPEVIRQKSK
                     DLNLEHISKAEIVFPDAYEKRQYYADLLYEIRKNKGMTPEQAYRLAADPLYLASLFIK
                     DGKADGEVAGAINTTGNVLRPAFQIVKTLPGVSVVSGAYIMIFKDSHWGENGMMIFAD
                     CAVNPDPTECQLTEIAVMSARTARILAGIEPRVAMLSFSTKGSATHTLIDKVSHATEI
                     AKEIDPSLMIDGELQADAAIVEEVAKRKCPNSPVGGKANVLIFPDLQSGNISYKLVQR
                     LGGADAIGPVLQGMAAPVNDLSRGCSSQDITNLIAITVIQAGGL"
BASE COUNT          455 a          299 c          339 g          510 t
ORIGIN      
        1 cgccagttga ataaatgcat agtgttgccc gaaggagagg aggaacgcac tttaattgca
       61 gcgaatgaag ttttaaaaga aaatttggct cgaattatac tcatgggcta tcctgaggta
      121 attcgtcaga aatcgaaaga cttaaatctt gagcatatat caaaggccga gattgttttt
      181 ccggatgcgt atgaaaagag gcagtattac gctgaccttt tgtatgaaat ccggaaaaat
      241 aagggaatga cacccgaaca ggcatatcgt ttagctgctg acccattata tttggcaagt
      301 ttatttataa aagatgggaa agccgacggt gaagttgcgg gtgccatcaa tactacaggc
      361 aatgttctgc ggcctgcctt tcagattgtt aaaactctcc caggtgttag tgtagtatcg
      421 ggagcttata taatgatatt taaagacagc cattggggtg aaaatgggat gatgattttt
      481 gccgattgtg ccgtaaatcc tgatcctaca gagtgccagt tgaccgagat tgccgtcatg
      541 tcggctcgca ctgcaagaat cctggctggc atcgaaccgc gggtagccat gttgagtttt
      601 agtacgaaag gaagtgccac gcatactctc atcgataaag tctctcatgc aaccgagatt
      661 gctaaggaaa ttgacccgtc cttgatgatc gatggagagc ttcaggccga tgctgcaatt
      721 gtagaagaag tagcgaaaag gaaatgtccc aatagtcctg tgggcgggaa agccaatgtt
      781 ctgatattcc ccgatcttca atcgggaaat atctcataca aattggtaca acgactcggc
      841 ggggctgatg caattggccc ggttttgcaa ggaatggccg ctccagtgaa tgacctttcc
      901 cgaggttgca gctctcaaga tattacaaat ctcatcgcta ttactgttat tcaggcaggt
      961 ggtttatagc ctttacaaag agatgcaatg gctatgaatt tatcctataa aattcttcca
     1021 aaatcacaat ttatatgaat ctttcgtttc aatgcaactg gtattttctc tgaactgcat
     1081 aatacgacga actctcattc ttttctttct cgatcggaat tatttttata gaaatgattc
     1141 ggttaataca aaatgccaac gtccaaattt gtttcgaaaa atttgatttt tgatttttta
     1201 gcaatataac caataaattt gtaattttgc tacgtatttc tttttttatc tatgtggcag
     1261 aagtattggc cgataagcac attatatcac aataacggga aaggtttgta ttcctgttgg
     1321 atgctctgaa aaatcatcaa ttacgattgt cagggtctgc tatcattgat tccttttcaa
     1381 tttacggttt tttttacaag actttgttcg acctttctat tcttgaagca gacttatctc
     1441 attttccgaa ttattatatc attcaatttg taaatattcc ttagtttttc tcgatctatt
     1501 ctcgataaat tccaatgaaa gctttgatat tcgatattcg acggttttgt gtcaatgatg
     1561 ggccgggcat tcgtacgacg gtttttttca aaggatgccc att
//
LOCUS       DAORPD010000451         1598 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_14955_length_1598_cov_2.875567, whole genome shotgun sequence.
ACCESSION   DAORPD010000451 DAORPD010000000
VERSION     DAORPD010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14955_length_1598_cov_2.875567"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="PK976_05785"
     CDS             complement(<1..541)
                     /locus_tag="PK976_05785"
                     /inference="COORDINATES: protein motif:HMM:NF013507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HRC67431.1"
                     /translation="MNSFSKITIAFCFLLMLFLQNVVLGQDTWIRKSDFAGSGRKGAV
                     ACTIGDKSYIGMGFDGIYHKDFWEYDPAIDSWIQLTDMPGQARAYAACFALGNKIYIT
                     TGYNGGFLLDTWVYNTTNHTWSAGINFPGGARQGCISTVIGDKAYVGLGYDGAYRKDF
                     YEFDPVSNSWTARNNFGGQG"
     gene            complement(1080..>1598)
                     /locus_tag="PK976_05790"
     CDS             complement(1080..>1598)
                     /locus_tag="PK976_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67432.1"
                     /translation="DGEREQITEKRYNNDKLIEENTYDDSGQFTSSKKWSYDDLGREV
                     KFENETEDQYILSESVYDDEGRLILRKVYGKNGNLFETWERIYNPNPYIEEYWETNRF
                     GRSRYHVNYDENNRELSRQVTNENGEIMLEVERLYRPDGLLDRQRIIIHGSGMRPSQF
                     EEYRNFYLLLNQ"
BASE COUNT          467 a          327 c          282 g          522 t
ORIGIN      
        1 tgccttgtcc tccaaaatta ttccttgcag tccaagaatt acttactgga tcgaattcgt
       61 aaaaatcctt cctgtaagcg ccatcgtatc cgagccccac ataagccttg tcgccaatta
      121 cggtagagat gcatccctga cgagccccac caggaaaatt tatacctgcc gaccatgtat
      181 gattcgttgt gttatacacc caagtgtcca ataaaaatcc gccattgtat cctgttgtga
      241 tgtaaatttt gtttcccaag gcaaaacaag cagcatatgc tctggcctgt cctggcatgt
      301 cggtaagctg gatccatgaa tctatagcag gatcgtattc ccaaaaatct ttatggtaaa
      361 ttccgtcgaa ccccatgcca atataagact tatcgccaat ggtacaagca acagctcctt
      421 ttctgccact acctgcaaaa tcactctttc taatccaagt atcctggcct aagaccacgt
      481 tctgaagaaa aagcatcaat aagaaacaga atgcaatggt aatcttgcta aaactattca
      541 tgggtcaatc aattaaaaac ttggggttta ggttaacttt cattcttcga gagccctgaa
      601 caagattctc tactttaaaa gcaactttgt cgagtagtag attataaatt tctaatagag
      661 taatttttat ggcatttact gtcacattaa taggaatata ggattctaat gatgaaataa
      721 ttggttgaga gttggtctta tggttctgtc tccatagctc gaaacttttt ccctttttgt
      781 aaatcgagtt ttggcaaata atcgattggt ggtgaaatga tagagccctt tcaccacctc
      841 tggtaccaga agaatgaaaa attaatggga agggcttcat catagctaaa tcattcatca
      901 tttataagct aattgcacat cttttcgtat tagtaaacaa agttcaaatt tatgaataaa
      961 attttgcaat gggctgaaac atcctcaaga tattttatat gaaagagaca atcagcgatc
     1021 gattcttgtt gtattgatgt catatttcag gagattcagc taaataggta caatgatcat
     1081 cattgattca ggagaaggta aaaattacgg tattcttcaa attgtgatgg gcgcatacct
     1141 gagccatgaa tgataattct ttgtctatcg agcaatccgt caggccggta aagacgttcg
     1201 acttcaagca tgatttctcc gttttcattg gtaacttgac gtgaaagttc tctgttgttc
     1261 tcatcataat tcacatgata tctcgagcgg ccaaatctgt tggtttccca atattcttct
     1321 atataagggt tgggattgta aattctttcc caagtttcga acagatttcc gttcttgcca
     1381 tatacctttc ggagaatgag gcggccttca tcatcataaa cagattcgct taaaatgtat
     1441 tgatcctctg tttcattttc aaattttact tcgcgtccaa gatcatcgta actccatttc
     1501 ttagaactgg tgaattgccc tgaatcatcg taagtatttt cctcgatcag cttatcatta
     1561 ttatatcttt tttcggtaat ctgttctctt tcgccgtc
//
LOCUS       DAORPD010000452         1598 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_14957_length_1598_cov_2.261180, whole genome shotgun sequence.
ACCESSION   DAORPD010000452 DAORPD010000000
VERSION     DAORPD010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14957_length_1598_cov_2.261180"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="PK976_05795"
     CDS             <1..181
                     /locus_tag="PK976_05795"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HRC67433.1"
                     /translation="DHKNLNTEVDFMYGRMTSIEMIAIAFWEQLKQPILAIGAQLYKI
                     RLEETENNFVEYFGQ"
     gene            273..890
                     /gene="folE"
                     /locus_tag="PK976_05800"
     CDS             273..890
                     /gene="folE"
                     /locus_tag="PK976_05800"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HRC67434.1"
                     /translation="MIDENSLGGYEKIDKYNYETLEKLSFHYYEILKLIGEDADREGL
                     LDTPMRVAKAMQFLTHGYDLEPKEILLSARFKEDYRQMVIVKDIEIYSLCEHHMIPFI
                     GKAHVAYIPNGYITGLSKIARVVDAYARRLQVQERLTTQIKECIQEALNPLGVAVVIE
                     AKHLCMAMRGVQKQNSVTTTSDFTGAFTRKETREEFIHLIGSELS"
     gene            complement(1149..1370)
                     /locus_tag="PK976_05805"
     CDS             complement(1149..1370)
                     /locus_tag="PK976_05805"
                     /inference="COORDINATES: protein motif:HMM:NF022596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HRC67435.1"
                     /translation="MKAGKWFIIIGLVLIIIGLMLYFLPSLFRWFGHLPGDIRIEKGN
                     TRIYFPLTSMIVISLVITVIINLFRFFKS"
BASE COUNT          521 a          265 c          333 g          479 t
ORIGIN      
        1 ggatcataaa aacctcaata cggaagtaga tttcatgtat ggcaggatga cttcgattga
       61 aatgatcgct attgcttttt gggaacaact aaagcaacct attttagcca taggagctca
      121 actttacaaa attcgacttg aagaaaccga aaataacttt gttgaatatt ttggacagta
      181 aatcaaacat tcctgatcaa gaaatgttac atcgccaata acccttagca tttaccaaca
      241 atatgaacaa gtccgacaag attattcacg atatgataga tgaaaattcg ttgggtggat
      301 atgaaaaaat cgacaaatat aattatgaaa cactcgagaa gctatcattt cattattatg
      361 agattttaaa actgatagga gaagatgccg atcgtgaggg tttgctcgat acccccatgc
      421 gggtagccaa agccatgcag tttcttacac acggctatga tctcgagccc aaggaaattt
      481 tgctctcggc tcgttttaag gaagattata ggcaaatggt gatcgtaaaa gacatcgaaa
      541 tttacagcct gtgcgagcac cacatgattc cgtttatcgg aaaagctcat gtggcttata
      601 tacctaatgg atacatcacg ggtttgagca agattgcccg agtggtcgat gcttatgctc
      661 gtcgcctgca ggttcaggaa aggctaacta cccaaatcaa ggaatgcata caagaagctt
      721 tgaatcccct aggtgtagct gtagttatcg aagctaaaca cttatgcatg gccatgcgtg
      781 gtgtacaaaa acagaattcc gtaaccacca cttccgattt tacaggtgct tttacacgga
      841 aagaaacacg tgaagaattt atccacctca ttggttctga gttatcatga gttttgaacg
      901 taatttgttg agggataccc cttacctgtg ataaagtgcg aggtgagagg gatttttgat
      961 aaatggcgaa aaccatagag gtgggtgatt ggaaagcatg aattgttgat aagttggaat
     1021 tagtaagatg cagggtcata aattcgagtt gcaacgctgg cgtagtgaat ctaaatggaa
     1081 ttcgaattct tagaatgtta tttttgtgtt taatagggcg atatatgata gcttctttgc
     1141 actctttttc aagatttgaa aaaccgaaat aaattaatga tgacggtaat aacaaggctt
     1201 atgacaatca ttgaagtaag agggaaataa attcgagtat ttcccttttc gatgcgaata
     1261 tcgcctggaa gatgtccaaa ccatcgaaat aatgacggta agaagtataa catcaatccg
     1321 atgataatca acacaagtcc tataataatg aaccatttgc ctgctttcat attttcaacc
     1381 aattcgctgc gatgataatt attttaattt acgtgataaa aaatgaaata atatttttta
     1441 atgagttttt tattgaaaca ttgcatcaga tttttgaact tgtctaacct tgatgctatt
     1501 atggaatttt gaggaattta tggacttgaa ggctgagtcg ccattgcgga aatttcttga
     1561 tataatcgat aatggcagga gttatttgtt gttgtcgg
//
LOCUS       DAORPD010000453         1598 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_14958_length_1598_cov_2.149060, whole genome shotgun sequence.
ACCESSION   DAORPD010000453 DAORPD010000000
VERSION     DAORPD010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14958_length_1598_cov_2.149060"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..953
                     /locus_tag="PK976_05810"
     CDS             <1..953
                     /locus_tag="PK976_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HRC67436.1"
                     /translation="RTQYGIVGCAAVDDYFNGVIKRHELTRKDKEEDRMKHVMITNAN
                     LEPVFFTYPAVNEIDKIVDKIVKSTEPVYDFIAPDGIGHHFWLIESDETIQEIVRLFA
                     KIPNTYVADGHHRTAAAALVGAEKRKMNPNHRGDEEYNFFMAVHFPDNQLRIIDYNRV
                     VKDLNGLSKEEFIEKIKQHFVIAEKGQQPYKPFHLHEFSMYLDGTWYSLEALGHTYNE
                     NDPIEVLDVTILSRYVLDEILGIKDLRTDKRIDFVGGIRGMQELSRRVDSGEMAVAFA
                     LYPVTLKQLIAIADSGKIMPPKTTWFEPKLRSGLVVHLLD"
     gene            1242..>1598
                     /locus_tag="PK976_05815"
     CDS             1242..>1598
                     /locus_tag="PK976_05815"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRC67437.1"
                     /translation="MNRLSDKVAIITGAARGMGKEEAKLFAKEGAKVVVADIIENEAI
                     EVAKEIAENGGEAMACKLDVSNAKNWERMVEEISNKWGKIDILVNNAGILSLAGVEEA
                     TEEQWDKVMAVNAKGNF"
BASE COUNT          530 a          326 c          326 g          416 t
ORIGIN      
        1 gtcgtacaca atatggaatt gtgggatgcg ccgccgttga cgattacttc aatggcgtta
       61 tcaaacggca cgaactcaca cgaaaagaca aggaagaaga ccgaatgaaa cacgtaatga
      121 tcacgaatgc taatctcgaa cccgtcttct ttacttatcc tgccgtaaat gaaatagata
      181 aaatcgtaga caaaatcgta aaatcaaccg agccagttta cgattttatt gcccccgatg
      241 gaattggaca tcatttttgg cttatcgaaa gcgacgaaac tattcaagaa atagttcggc
      301 tttttgccaa aattccgaat acctacgtcg ccgatgggca ccaccgtacg gctgctgctg
      361 ctctcgttgg agccgaaaaa agaaaaatga atcccaatca tagaggcgac gaagaatata
      421 acttcttcat ggcagttcat ttccccgaca atcaactgag aatcatcgac tacaaccgag
      481 tagtaaaaga tttgaacggt ttgtcgaaag aagaattcat tgaaaaaatc aaacaacatt
      541 ttgtaattgc cgagaaaggc cagcaacctt acaaaccttt tcaccttcat gaattcagca
      601 tgtatctcga cggaacatgg tattccttag aagccctcgg acatacttac aatgagaatg
      661 atccaatcga agttctcgac gtaaccattc taagccgcta tgttctcgac gaaatcttag
      721 gaatcaagga tctgcgtacc gataaacgca tcgactttgt gggaggcatc agaggaatgc
      781 aggaattgag ccggcgggtc gacagcggtg aaatggccgt cgcttttgcc ttatatcccg
      841 ttacgctaaa acaacttatt gctatagccg attcaggtaa gattatgcct ccaaaaacta
      901 cctggttcga acctaaatta cgctcaggcc tcgtagttca tctgctcgac tgatttttag
      961 cagaaatcct tgctcattat cctaatttat aagtaactca ttcgatctta taatacaact
     1021 aatagagtcc taaccctcca gtatgggctc tattttttat tcttattcat tatccctcct
     1081 cgattcttta tggttatttt tatgaatgga atatgaacct ttcaaacgtt gtattgttaa
     1141 ttcattaaca aaagaaaatc atcaatattt cttttaattt tcagtaaaaa gaatcagaaa
     1201 tcaattaatt ttagatttca attttaaaaa caggaggaaa tatgaacaga ttatcggata
     1261 aagtagccat aataacggga gctgccagag gcatgggcaa agaagaagcc aagttatttg
     1321 ccaaagaagg agccaaggta gttgtggccg acatcattga aaatgaagcc atagaagtag
     1381 ccaaagaaat cgccgagaat gggggcgaag ccatggcatg taaactcgac gtttccaatg
     1441 caaaaaactg ggaacgaatg gtagaggaga taagcaataa atggggcaag attgatattc
     1501 ttgtcaacaa tgcaggaatc cttagcctgg caggcgttga agaggctact gaagaacaat
     1561 gggacaaggt aatggccgtc aatgccaaag gcaatttc
//
LOCUS       DAORPD010000454         1598 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_14961_length_1598_cov_2.012962, whole genome shotgun sequence.
ACCESSION   DAORPD010000454 DAORPD010000000
VERSION     DAORPD010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14961_length_1598_cov_2.012962"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="PK976_05820"
     CDS             <1..614
                     /locus_tag="PK976_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67438.1"
                     /translation="QSNLINIASLKDQLGYDYMVMLRPDYRPSEKILCRVKKISKAKV
                     HLVSEVANPQWLSPPQEGLYTMKNIGIYSHPSGNRFCLAYHPIFGVDLIPLNSVVDAG
                     FEKSPFLQCFVYTDDYEIPRFEPVPAGLWPGKEKELYFDSYDSKMDCMRGPKTIMIVN
                     DGLGNEYEISLGNSKYITMPFGNPVKCRVKKIKKGRPVFELIP"
     gene            complement(1180..>1598)
                     /locus_tag="PK976_05825"
     CDS             complement(1180..>1598)
                     /locus_tag="PK976_05825"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HRC67439.1"
                     /translation="VMPLIGWLIGSSFEQTLKSYDHWIAFLLLSFLGVRMIVSSFRGK
                     KPPKPFNPLNLPTIMGLSVATSIDALIVGFSLSLIEANLIFAVFSIGFITLIAAMLGI
                     SIGKKTSPLLGKKMGAVGGVILIVIGLKILLSHILV"
BASE COUNT          493 a          305 c          314 g          486 t
ORIGIN      
        1 tccaatcgaa cctcatcaac atagccagtc taaaagatca attgggctac gattacatgg
       61 tgatgcttcg ccccgattat cgaccttcag agaaaatttt atgtcgagta aagaaaattt
      121 cgaaggcgaa agtacatctt gtttctgaag tggcaaatcc acagtggcta tcccctcctc
      181 aggagggttt atataccatg aagaatatag gaatctattc ccatccttct ggaaatcgat
      241 tttgtttggc ttatcatcca atttttggtg tagatttaat ccctttaaac tcagtagtag
      301 atgcaggctt tgagaaatcg ccatttttac aatgtttcgt atataccgat gattatgaaa
      361 ttcctcgttt tgagccagta cctgctggtt tatggccggg aaaggaaaag gaactatatt
      421 tcgattctta tgattcgaag atggattgta tgcgcggtcc taagactatc atgatagtaa
      481 atgatggact tggaaatgaa tatgaaattt cgttgggaaa ctcgaaatat attacaatgc
      541 cttttggaaa tccagtgaaa tgcagggtta agaagattaa aaaaggccga cccgtatttg
      601 aactcattcc ctgatctggc taaacttcgg tcgaaatgct ttttttactt gaataaatct
      661 tttcgcaggt tagggtttat ttagaaaact catcgttatg caaatgaatt tgagcccaat
      721 acccaggcaa taccttacag gctaattgaa tgagatgaaa attcaggctg agcttgtttt
      781 gaacagtttg ctatatcacc cagcggaggc ccttatcatt ggagaaccca gaccagatgc
      841 ctctgttttc tcggaggaga atgaacaagg aatggcttcg gcactgttaa aacctaacag
      901 gtctatgacc tattaggttt acacttatct gaaaatcttt acacaaaaat acgacaattg
      961 aaattccagc aatagcgttt tagagaagat tccttgttta cctcttttag aatagaattc
     1021 tggtatggga gagaaaaatc gcttgcatga tcgtttgcct tgcccacttg atgaaaattt
     1081 ctggatcatc tgtcgcatta atcctcaatt gtcgattttg tttcgtctcg atagatacgg
     1141 tgtgaattat gggattataa cttgaagtat ttgttttaac tagacaagaa tatgcgaaag
     1201 aagtattttt agtccaatga ctattagaat aacacctccc actgctccca tttttttccc
     1261 gagaagcgga ctggtctttt tccctattga aatcccgagc atagctgcta tgagagttat
     1321 gaaccctatc gaaaaaacag caaatattag attagcttcg atcaatgaca agctgaatcc
     1381 tacaatcaat gcatcaatac tcgtggcaac cgaaagcccc atgatagttg gcagattgag
     1441 aggattgaaa ggttttggcg gttttttccc cctaaaactt gaaacgatca ttcttactcc
     1501 taaaaagctc aacaatagga aggcgatcca gtgatcataa ctttttagag tttgctcgaa
     1561 agatgaacca atcaaccatc caatcaaagg catgacag
//
LOCUS       DAORPD010000455         1594 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_15013_length_1594_cov_2.154646, whole genome shotgun sequence.
ACCESSION   DAORPD010000455 DAORPD010000000
VERSION     DAORPD010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15013_length_1594_cov_2.154646"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="PK976_05830"
     CDS             complement(<1..213)
                     /locus_tag="PK976_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-methyltransferase TrmM"
                     /protein_id="HRC67440.1"
                     /translation="MFRFKQFEIEDNQSTMKVCTDSVILGAWVAIGEAERILDIGTGC
                     GLLSLMCAQRSKAWITGIDIHFPSIEQ"
     gene            complement(334..864)
                     /locus_tag="PK976_05835"
     CDS             complement(334..864)
                     /locus_tag="PK976_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67441.1"
                     /translation="MIEQEDYIKTGQILRTYGISGALAISVELNYNPFEHARIDFMFV
                     AIDGSFVPFHILNSEPGPSGRWIIYLEDIPDPGKASFLCKRSYYIHKSYFPSIWPTRF
                     PAQRIEGFTIVDIHRGKLGEVQRLGGTPDNPLVIFDFQNMEVLLPINEHFIKNIDFNN
                     KSIKTDIPDDLLFLNQ"
     gene            complement(943..1467)
                     /locus_tag="PK976_05840"
     CDS             complement(943..1467)
                     /locus_tag="PK976_05840"
                     /inference="COORDINATES: protein motif:HMM:NF011094.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HRC67442.1"
                     /translation="MAVRMRLQRFGKKGQPFYHIVVADGRAPRDGRVIEKLGTYNPLP
                     NPAEIQIDMDRAIYWLNVGAQPSDTVKALLSYKGVLLKRHLLRGVEKGALTAEQAEAK
                     FQEWSAEKEAKIKAKITSKEAFQRDHLKSKLEAERKIAEERARQIAKKRSTETLNEEL
                     SNSEEAEISPSTEE"
BASE COUNT          458 a          322 c          312 g          502 t
ORIGIN      
        1 ttgttcaatc gaagggaaat gaatatctat acctgtaatc catgccttgc ttctctgagc
       61 acacatcaaa gaaagcaatc cgcatcctgt tccaatatcc aagatccgct cagcttcacc
      121 tatagctacc catgcaccca atataaccga gtcggtgcat accttcatgg tactctggtt
      181 atcttctatc tcgaactgct tgaaacgaaa cattcttatg ataactatga taataacagt
      241 ctttattcaa ataggaacca aatttcgaaa gttttcggaa gataactttc cgcacttttt
      301 gtaggaaaac gatttcacgg ttttgaactt gtcttattga ttcaggaaaa gcaaatcatc
      361 aggaatatct gttttgattg atttattgtt aaagtcgata ttctttataa aatgctcatt
      421 gatagggagg aggacttcca tattctgaaa atcgaaaata accagagggt tatcgggagt
      481 gccgccaagg cgttgtacct cgccaagttt acctcgatga atgtcgacga ttgtaaatcc
      541 ctcaattcgt tgggcaggaa atcgtgttgg ccatatcgag ggaaaatagc ttttatgaat
      601 gtaataggaa cgtttgcata aaaaggaggc tttcccagga tcgggaatat cttccagata
      661 aataatccag cggccacttg gtccgggttc tgaattcagt atatgaaagg gaacaaagct
      721 accatcaatg gcaacgaaca taaagtctat tcgggcgtgt tcgaaagggt tatagtttaa
      781 ctcgactgag attgcaagtg cgcctgaaat gccataagtt cggagtattt gtccggtttt
      841 gatatagtct tcttgctcga tcatgaagtg aggattcaag cttctaaatt aacaaaaaac
      901 ccggtgatgg ccgaccgggt tcaaagtatc accgaaaaga tgttattctt cagttgaggg
      961 tgagatttca gcttcttccg aattcgaaag ttcctcgttg agggtttcag tactgcgttt
     1021 tttggcaatt tgccttgctc tttcttcggc aattttgcgt tcagcttcca gtttgctttt
     1081 cagatgatca cgctggaagg cttctttgct tgtaatctta gccttgattt tggcttcttt
     1141 ttcagcagac cattcctgaa atttagcttc ggcctgttcg gcagtcaaag ctcccttttc
     1201 aacacccctt aacaaatgtc ttttcagaag tacaccctta tatgacaata aggctttaac
     1261 cgtatcagaa ggttgagctc cgacgttcaa ccagtaaatt gctctatcca tatcgatctg
     1321 gatttcggca ggattaggaa gggggttata cgttcccagt ttttcgataa cacgtccatc
     1381 gcgtggtgca cgaccatctg ctactacaat atgataaaaa ggctgaccct ttttaccaaa
     1441 tcgttgtaat ctcattctaa cagccataaa ttattgtttt ttaaatggta ataaaatatc
     1501 ttgtttgcaa aaaacaaggg tgcaaatttg caaaaaaaat ttaaattctc ttaaataatc
     1561 ggtattttcc ttttaaaaat catcatccgt ctga
//
LOCUS       DAORPD010000456         1591 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_15063_length_1591_cov_1.918620, whole genome shotgun sequence.
ACCESSION   DAORPD010000456 DAORPD010000000
VERSION     DAORPD010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15063_length_1591_cov_1.918620"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="PK976_05845"
     CDS             <1..621
                     /locus_tag="PK976_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HRC67443.1"
                     /translation="GYETWFRLTGRFNAYNLTATYGAAILLGETSEEVLRVMSNLHPA
                     EGRLDILKSEEGINVIVDYAHTPDALQNVIETVRDIHAGAGRLIVVCGAGGDRDKSKR
                     PKMAAIAAENADHLILTSDNPRSEDPLQIIEDMKAGLNPSLSYRVLSIPDRREAIHIA
                     CMIAKTGDVILVAGKGHEKYQEINGVKYPFDDKEILSQYLHLIKPR"
     gene            622..>1591
                     /locus_tag="PK976_05850"
     CDS             622..>1591
                     /locus_tag="PK976_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HRC67444.1"
                     /translation="MLYYLFTYFDLAFDFPGAGLFQYISFRAASAVILSLIITLIFGG
                     TIIQYLKKRQIGEEIRDLGLEGQLQKRGTPTMGGLMIIAGIVIPTLLFARLDNVYIIL
                     MIITTLWLGFIGFVDDYIKVFKKNKEGLQKRTKLAGQVILGIVVGVTMYFSPDIVIRE
                     KTETHAFLETTNVLRESNYERAQEKFTAESTKSLKTTIPFVKNNEFDYAVLVNWIGKE
                     ARSYAWLVYIPIIILIITAVSNGVNLTDGLDGLAGGTSAVAASTLGILAWVSGNIIFA
                     DYLNIMYIPNTGELAIFISSLVGATIGFLWYNTYPAQVFMGDTGSLT"
BASE COUNT          455 a          334 c          351 g          451 t
ORIGIN      
        1 ggatatgaaa cttggttcag attaacgggt cgtttcaatg cctataatct gacggcaact
       61 tatggtgcag ctattttact gggagaaacc tctgaagagg ttttacgggt gatgagcaat
      121 ttgcatcccg ccgaaggccg attggacatt ttgaaaagtg aagaaggcat aaatgttatc
      181 gttgattatg cacacacacc cgatgcttta cagaatgtaa tcgaaaccgt gcgtgatata
      241 catgcaggtg ctggtcgctt gatcgttgtg tgcggcgccg gaggagaccg cgataagtca
      301 aaacgcccga agatggcagc catagccgcc gaaaatgccg accatttgat acttacctcc
      361 gataatcccc gcagcgaaga tcctttgcag attatcgagg atatgaaggc tggtttgaac
      421 ccctcacttt cctacagagt tcttagcatc cccgaccgcc gtgaagccat ccatatagcc
      481 tgcatgatag ccaaaactgg cgatgtaatc ctcgttgcag gtaaaggaca cgaaaaatat
      541 caggaaatca atggcgtgaa atatcctttc gatgataaag aaattctttc acaatacctc
      601 catctaataa aaccacgata aatgctatat tatctcttca catatttcga tcttgcattc
      661 gatttccccg gagccggcct tttccagtat atatcattca gggcagcgtc ggcggttatt
      721 ctttccttga ttattacact catcttcggt ggaacaatta tccaatatct aaagaaaaga
      781 caaattggag aagaaatacg cgatcttggt ttggaaggtc agcttcaaaa aagaggtact
      841 cctactatgg gcggtttaat gattattgct ggaattgtta ttccaaccct gctctttgca
      901 cgtttagaca atgtctatat tatcttaatg ataatcacta cattatggct tggtttcatc
      961 ggatttgtag atgattatat taaggtattc aagaaaaaca aggagggtct tcagaaaaga
     1021 accaagcttg ccggacaggt aattttagga atagtagtgg gagttaccat gtatttcagc
     1081 cctgacatag tgatacgtga aaaaactgaa actcatgcat ttctggaaac taccaatgtt
     1141 ctgcgtgaga gtaattatga acgtgcccaa gaaaaattta ctgccgaatc taccaaatcg
     1201 ctgaaaacaa ccataccttt tgtaaaaaac aacgaatttg attatgctgt attggtgaac
     1261 tggataggca aagaagcacg gagctatgcc tggttggtat atatccccat cataattctg
     1321 atcatcaccg cggtatcgaa cggcgtaaat ctcacggatg gtttggatgg cctggcaggc
     1381 ggcacttcgg ctgtggctgc ctccactctt ggtattcttg cgtgggtctc gggaaatatc
     1441 atttttgccg attatctcaa tatcatgtat atacccaata caggagagct ggctattttc
     1501 atcagttctc tggtaggtgc tacgataggc tttttgtggt acaacaccta tccggctcag
     1561 gtatttatgg gagatactgg ctcgctcact c
//
LOCUS       DAORPD010000457         1591 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_15067_length_1591_cov_1.855469, whole genome shotgun sequence.
ACCESSION   DAORPD010000457 DAORPD010000000
VERSION     DAORPD010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15067_length_1591_cov_1.855469"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1418)
                     /locus_tag="PK976_05855"
     CDS             complement(<1..1418)
                     /locus_tag="PK976_05855"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HRC67445.1"
                     /translation="MLFSIEVNNKVITAQRGETILECLKRNGVFVPTLCHIEGYSPTG
                     ACRICVVEVEGRPDLVPSCSHPVEEWMKIKTHSPRVIRARRAIAEMLLSNHPDDCLYC
                     GRNGNCELQNLAIDLNLREKRFSGSKNTLKNDYASVSIMREPSKCILCGRCVRLCETE
                     QVVAAVDFIGRGNQMTIGTSYNHGLSSRNCHHCGQCILICPTGALSEQQHIAKVQEAL
                     HNPEKHLVAQFSPALSVGLNENLGIKAGKTADHIIVAALSKIGFSKVFDTGLATDLNI
                     LENASLLAKQIREGESMPMFTSCCPSWLHYAETFHPSILPCISPVLSPQQIMSVLIKK
                     IYASEMRLRAEDIYTVGLMPCTAKKYEILRQEMLIDDLNMTDAVLTTRELARFIKLYG
                     IDVNNIEPMLPHSPFHIRSTAAKLYGVSGGVTEAILRTVYFLLTGVELEPYKIGEVRS
                     GSGPKFFTMEAGKYKVKTAVVSG"
BASE COUNT          461 a          376 c          298 g          456 t
ORIGIN      
        1 ccgcttacca cagcagtttt tactttgtat tttcctgcct ccatggtaaa aaatttgggc
       61 ccgctgcctg aacggacttc tccaatttta taaggttcaa gctccacgcc cgtcagtaag
      121 aaatacaccg tacgtaaaat ggcttcagtt actccacccg atactccata aagtttggca
      181 gccgtacttc gaatatgaaa cggactatgt ggcagcatgg gctcgatatt attcacatct
      241 attccataca acttgataaa cctggctaat tcacgcgtag taagcacggc atcagtcata
      301 ttaaggtcat ctatcaacat ttcctgcctc aatatctcat actttttagc tgtacaaggc
      361 atcaacccaa cagtgtaaat atcctccgct ctcagacgca tttcgctggc atagattttc
      421 ttgatcaaga cgctcattat ctgctggggc gacaatacag gactgataca aggtaaaatc
      481 gagggatgaa aggtttcggc ataatgcaac catgaaggac agcaggaagt aaacataggc
      541 atgctttctc cctcacgaat ttgtttggcc aacaaagaag cattttccaa aatattcaag
      601 tcggtagcta aacctgtatc gaaaacctta gaaaagccaa ttttactcag tgctgcaact
      661 ataatatgat ccgcagtttt acctgccttg attccaagat tttcgttaag ccccacgctc
      721 aatgcagggg aaaattgcgc taccagatgt ttctctggat tatgcaaagc ttcttgcact
      781 tttgcaatgt gttgctgttc cgagagagca cccgtaggac agatcaatat acattgcccg
      841 caatgatgac aatttctcga acttaatcca tgattatatg aggttcctat agtcatctgg
      901 ttgcctctgc caataaagtc aacggctgca accacctgct cggtttcgca aagtctgaca
      961 caacggccgc ataatatgca tttggatggt tcacgcatga tgctaacgct tgcataatca
     1021 ttctttaaag tattcttact tccgctaaaa cgtttttccc ttaaattcag atcgatagca
     1081 aggttttgta attcacagtt tccattgcgt ccacaataaa ggcaatcgtc gggatgatta
     1141 gagagaagca tttccgctat agctcttctt gctctgataa cacgaggtga atgggttttt
     1201 attttcatcc attcttccac cggatgggaa caagatggca ctaaatcggg ccggccttcc
     1261 acttctacca cacatatcct gcaagcaccc gttggagaat atccttcaat atgacaaagc
     1321 gtgggaacaa ataccccatt ccttttcaag cattcaagga tggtttcacc cctttgggca
     1381 gtaatgacct tattattaac ttctatcgaa aacaacatgg aacttctttt aattatagat
     1441 ataaaccctt gattcaatga acagaaactg catcgaattt gcaaacatca tagcatatac
     1501 cacagccaat acacttgtcc tgcacaatga agtgtggatt gcgctgagtg cctataatag
     1561 ccgaagtagg acacttgcgg tagcatattg t
//
LOCUS       DAORPD010000458         1590 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_15083_length_1590_cov_2.507492, whole genome shotgun sequence.
ACCESSION   DAORPD010000458 DAORPD010000000
VERSION     DAORPD010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15083_length_1590_cov_2.507492"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1094
                     /locus_tag="PK976_05860"
     CDS             <1..1094
                     /locus_tag="PK976_05860"
                     /inference="COORDINATES: protein motif:HMM:NF040069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6340 family protein"
                     /protein_id="HRC67446.1"
                     /translation="SYKTNFTCIFIMLKNCFMFSMNRFQWRFKLFGLLTIGLTLQSCI
                     TQRKISFSTLTPAEITLSPEISEVCLINHGISEPEDSNGVFYGFAGQLYYDSTKYDTL
                     ISWAVMDGLTDGFATSQRLVISGEPLLWLRSSIEKETFAFISDTMMDSFCPSNIADAS
                     LIIDKMSIFDNFDYFLMDNGLYYMQLTALAAIGFQFYDLQNKQLVDQYISIDTLSYST
                     YGYSWEKTIATLPGRAELYQDIAYDIGKKYAWRLTPGLKKHQRFYYLTNDKDIQKGHE
                     YANQGLWATAARYWIKLTDNKNKRKAYLSAFNMALASEMEGQLDIALYWIDRALEKYH
                     TAEAVKYKTILEQRQKDLDLLIKQMSINP"
     gene            1107..1541
                     /locus_tag="PK976_05865"
     CDS             1107..1541
                     /locus_tag="PK976_05865"
                     /inference="COORDINATES: protein motif:HMM:NF015444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HRC67447.1"
                     /translation="MEFEQDNELIVIRLWKDEEILSSLTEFCNSHQINAGWISGIGAA
                     EKIIAGYYDFIRKEYVWKEFEGVHEIVPLTGNISQIGEKVFLHLHVNFTDHDLRSYGG
                     HLKSAIVNPTLEVIVCKLTRPLVRFYDIETGLYLLKLSNNNI"
BASE COUNT          509 a          299 c          309 g          473 t
ORIGIN      
        1 cttcgtataa aacaaatttt acttgcatct tcattatgct taaaaattgt ttcatgtttt
       61 cgatgaatcg cttccaatgg agatttaagc ttttcggtct tctgaccatt gggcttactt
      121 tacaaagttg tattacacag cgcaaaatca gcttttcgac tttgacacct gccgaaatta
      181 ccctatctcc cgaaatatcg gaagtttgtc tgattaatca tggtatttct gaaccagagg
      241 attcgaatgg agttttttat ggatttgccg gacaactata ttatgattcg accaaatacg
      301 ataccttaat atcatgggcg gtaatggatg gtttgaccga tggttttgca acctcgcagc
      361 gattggtaat atcaggggaa cctcttttat ggctacgtag cagtattgaa aaggaaactt
      421 ttgcattcat ttccgataca atgatggatt ctttttgccc ttccaacatt gccgatgctt
      481 cattgatcat cgataagatg agtatattcg ataatttcga ctattttttg atggataacg
      541 gattgtatta tatgcaatta actgctctgg cagcaatagg tttccagttt tacgacttgc
      601 aaaacaaaca attagtcgac caatatattt cgatagacac tctttcatat tcgacttatg
      661 gttattcatg ggaaaaaacc atcgccactc tgccaggaag agcagaactt taccaagaca
      721 ttgcttacga tattgggaaa aaatatgcat ggcgtctcac gccaggacta aaaaagcatc
      781 aaagattcta ttacctcact aatgataaag acattcaaaa aggacacgaa tacgccaatc
      841 agggtctttg ggctaccgct gcccgatact ggataaagtt gaccgataat aaaaacaaac
      901 gcaaggcata tttatcagct tttaacatgg ctctggcaag cgaaatggaa ggtcagctcg
      961 atattgccct ctactggata gatagagccc tcgaaaaata ccacaccgca gaagctgtga
     1021 aatataagac catactcgaa caacgacaga aagatttaga tttattgatt aaacaaatga
     1081 gcattaaccc ttaattattt tcaaaaatgg aatttgaaca agataatgag ctaattgtga
     1141 taagactctg gaaggacgag gagattctat cgtccctgac tgagttttgt aacagccacc
     1201 aaatcaatgc aggttggatt tctggaattg gtgcagccga aaaaattatt gcaggttatt
     1261 atgattttat caggaaagaa tatgtttgga aagaattcga aggagtgcat gaaattgtcc
     1321 ccctaacggg aaatatcagt cagatagggg aaaaggtgtt tttgcattta cacgtcaatt
     1381 ttaccgacca cgatctaagg tcgtatggtg ggcatttaaa aagtgccatt gtgaatccca
     1441 ccctcgaagt aatagtttgc aagctcactc gtcctcttgt cagattctac gatatagaaa
     1501 caggtttata cttgctaaaa ttatccaata acaatattta atttatcgta agatgataaa
     1561 aacaaacccc cgagtttcgc aactgggggg
//
LOCUS       DAORPD010000459         1588 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_15119_length_1588_cov_2.327462, whole genome shotgun sequence.
ACCESSION   DAORPD010000459 DAORPD010000000
VERSION     DAORPD010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15119_length_1588_cov_2.327462"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="PK976_05870"
     CDS             complement(<1..391)
                     /locus_tag="PK976_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67448.1"
                     /translation="MLQKKSISFLCLVISFIVGKQVFSQEIPCSQIRDDTLWLCCGTK
                     ILLDNELVLISTDTIIIGDDISRVYFLKDPYARSRILYDSLKSKAGKTELTKLAYQLL
                     FPESGAVSKNNETLSEPPEENEYLPFAD"
     gene            complement(467..>1588)
                     /locus_tag="PK976_05875"
     CDS             complement(467..>1588)
                     /locus_tag="PK976_05875"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HRC67449.1"
                     /translation="VDRAYIAAIKNLSLYPEIIARYKNLKIVYTPIHGTGVRLAPWAL
                     QEFGFEQIFTVDEQMMVSGDFPTVHSPNPEEPAALDLAIKKARETGADLVMGTDPDGD
                     RVGIAVKDEKGEFVLLNGNQTASLLIYYILKAWKAKRRLKGNEFIVKTIVTSELIKEI
                     AQKEGVKCFDVLTGFKYIADLIRRLEGSMHFIAGGEESYGFLTGDFVRDKDAISTCCM
                     IAETAAWAANQGKSLYRLLKEIYLEYGFYKETLLSITNKGIAGAEEIRQMMENYRANL
                     PEQLAGIKVTVIKDYLCQTEINLITGEKIHIDLPKSDVLQFFLEDGSKITVRPSGTEP
                     KIKFYFGAKTILHHIEDYEKTNQFLSQKIEDIKHDMGLA"
BASE COUNT          434 a          315 c          334 g          505 t
ORIGIN      
        1 tatcggcaaa aggcaaatat tcattttctt cgggcggttc ggagagggtt tcattatttt
       61 ttgatacggc tcctgattct ggaaacagaa gttgataagc cagcttggtg agttcagtct
      121 tacctgcttt cgattttaaa gaatcataaa gtattcgtga acgtgcataa gggtctttca
      181 agaaataaac tcttgagatg tcatctccaa tgataatggt gtcggtagaa atcaatacca
      241 attcgttgtc gagcagaatt tttgtaccgc aacaaagcca caaagtatcg tcacggattt
      301 gcgaacatgg tatttcctgg ctaaaaactt gcttcccaac tataaacgag ataactaaac
      361 ataaaaatga tatggatttc ttttgcaaca catcatcatg aattacagag caaacgccag
      421 ctttgatttt ttatgatacg aaaaaagagg ataataaaac gatgcattac gcaagaccca
      481 tatcatgctt gatgtcttcg attttttggc ttagaaactg gttcgtcttt tcataatctt
      541 caatatggtg aagaatggtt ttggctccaa aatagaattt aatcttaggt tcggtccccg
      601 aaggtcttac ggtgattttg ctgccatctt cgaggaaaaa ttgaagcaca tccgatttgg
      661 gcaagtcaat gtggattttt tcgcctgtaa tcaggtttat ctctgtttgg catagataat
      721 cttttattac tgtaaccttg ataccggcga gctgttcagg aagatttgcc ctgtaatttt
      781 ccatcatttg acggatttct tcggctccgg caattccttt attagtaata gaaagaaggg
      841 tttccttata aaatccatat tcgagataga tttccttgag aaggcgatag agtgatttac
      901 cctgattagc agcccaggca gctgtttcgg ctatcatgca gcaggtgctt atggcatctt
      961 tatctctgac aaaatcgccc gtaaggaaac cataactttc ttctcctccg gcaatgaaat
     1021 gcatggaacc ttccaaacgt ctgattaaat cggcaatata cttaaaacct gtaagtacat
     1081 cgaagcattt cactccttcc ttttgggcga tttcctttat aagttcggag gttacgatgg
     1141 tttttacgat gaattcgttt cccttaaggc gtcttttggc tttccatgct ttcaaaatgt
     1201 aatagataag cagagaagca gtttgatttc cattgagaag cacgaattca cctttttcgt
     1261 ctttcacggc gatgcccacg cggtctccat cggggtcggt tcccatcacg aggtcggcac
     1321 cggtttcccg ggcttttttg atggcaagat cgagggctgc gggttcttca ggattggggc
     1381 tatgtacggt tggaaaatct cctgatacca tcatttgttc atctacggta aaaatttgtt
     1441 cgaaaccaaa ttcttgcaac gcccatggag caagtctcac tcctgtgcca tgaataggag
     1501 tatagactat tttgagattt ttgtaccggg caatgatctc tggatagagt gaaagatttt
     1561 ttatagcggc gatataagcc ctgtcaac
//
LOCUS       DAORPD010000460         1586 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_15158_length_1586_cov_1.919007, whole genome shotgun sequence.
ACCESSION   DAORPD010000460 DAORPD010000000
VERSION     DAORPD010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15158_length_1586_cov_1.919007"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..919)
                     /locus_tag="PK976_05880"
     CDS             complement(<1..919)
                     /locus_tag="PK976_05880"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HRC67450.1"
                     /translation="MKTDKILEVSFRCNECLATDTSVSVVLGDRTTPWRMMKDGEFAN
                     EDWEMMIRKQPFEIVFTYREKPAVVFSGYFASNESNRGFHFVIGDEKFITGGGMRALP
                     LNIKGRLLKLENRPWYGYGFEADQLNYSIPLLIMGSGYAIFFDNTAKGYADIGKTQSK
                     ILKFGTRGGRMAFYLIVGENLQEITQNFTLLTGHQPLPPLWTFGHLLSRMAYRTQAQT
                     DSIVRATIQNGYPLDAIILDLFWFGDTLFHTLGNLDWYRPNWPDPIQMMNSFREMGVK
                     TVVITEPYIVKGSHTFEEADRLGILGKNKD"
     gene            complement(1286..>1586)
                     /locus_tag="PK976_05885"
     CDS             complement(1286..>1586)
                     /locus_tag="PK976_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67451.1"
                     /translation="VNLVTSMGVSPWPGFPPIVPRIPDILLINAIYIFGQRYILFFTH
                     FTPFPFDFYNILIGNGIINFVPSLISILLITSYSLLFNSSLEFAFVSYIILTIGG"
BASE COUNT          528 a          315 c          280 g          463 t
ORIGIN      
        1 catctttatt tttcccaaga attcccaaac gatcggcttc ttcgaaagta tgcgagcctt
       61 ttacaatgta aggttcggta attaccaccg ttttcacgcc catttcacga aacgaattca
      121 tcatttgaat gggatcgggc caatttgggc ggtaccaatc gaggttgcca agagtgtgaa
      181 ataaggtatc gccaaaccaa aacaaatcga gaatgatggc atcgagagga tatccatttt
      241 ggatggtagc tctgacgata ctgtcggttt gagcctgagt tcggtaagcc atacggctta
      301 gtagatgacc aaatgtccaa agaggaggca aaggttggtg tccggtaagc aaggtaaaat
      361 tttgcgtaat ttcctgtaaa ttttcaccaa caatcagata aaaagccatc ctccctcctc
      421 ttgtgccaaa ttttaaaatt ttactttggg ttttgcctat atcagcatag ccctttgccg
      481 tattatcgaa aaaaatagca taacccgatc ccataatcaa taatggaatg ctgtaattga
      541 gctgatcggc ctcaaaaccg taaccatacc atggcctgtt ttccagtttc aataatcgtc
      601 ccttgatatt caaaggaagt gctctcatac ctcctccagt aataaatttt tcatctccta
      661 taacgaaatg gaaaccccga ttcgattcgt tcgaagcaaa ataacccgaa aaaacaaccg
      721 ccggtttctc tcgataagta aatactattt cgaagggttg ttttcgaatc atcatttccc
      781 aatcctcatt ggcaaattct ccgtctttca tcattctcca aggagtagtt cgatcgccaa
      841 gaactacact taccgaagta tctgttgcaa gacattcatt acaccgaaaa cttacttcca
      901 aaattttgtc ggttttcaca acaaaacgat attcgccttc cgtagtatgt ataaaaagct
      961 gatgcccatt gctattcgat gataaatatc tttctgtatt ctgagcatga agcacatcat
     1021 gtataagaaa gaatataaac aaccgacaga tgcagttaaa tatccaataa gattgtttca
     1081 tgtgatgtta tagctatgac ttttctttaa aaaccgagct aaagtaaaga tttctcttgt
     1141 aaattcgaag atggactgtt gcttattaac tattaatcta cagattttta aagataatgt
     1201 taatggcatt ccctttcgtt gatttacgct gattgaattt gctattccac gcagattttc
     1261 tgtgaacatc agctatggaa atgatttaac cccctattgt caggataata tatgagacaa
     1321 aagcaaattc caaagaagaa ttaaacaaaa gagaatagct cgtaatcaat aaaatactaa
     1381 taagagaagg aacaaaattg atgattccat tccctatcaa tatgttataa aaatcaaagg
     1441 gaaagggtgt aaagtgagta aaaaacaaaa tgtatctttg cccaaaaata taaatggcat
     1501 taatcaagag tatatcgggg atacgaggta caattggagg gaaacccggc caaggactca
     1561 cacccattga tgtaaccaga tttact
//
LOCUS       DAORPD010000461         1584 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_15186_length_1584_cov_1.877698, whole genome shotgun sequence.
ACCESSION   DAORPD010000461 DAORPD010000000
VERSION     DAORPD010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15186_length_1584_cov_1.877698"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="PK976_05890"
     CDS             complement(<1..401)
                     /locus_tag="PK976_05890"
                     /inference="COORDINATES: protein motif:HMM:NF014750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HRC67452.1"
                     /translation="MNIEDFRKTHRLLKEYVHEWHPIYRGYNNRTLYINVDTLEFKEK
                     PVNEEMKEKFTGGKGFGLKLLWEGTLPTTKWNDPENEVVISPGPIAGITQYSGTGKSL
                     VVSISPTNFVMDSNVGGFFGPFLKFCGFDAL"
     gene            709..888
                     /locus_tag="PK976_05895"
     CDS             709..888
                     /locus_tag="PK976_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRC67453.1"
                     /translation="MDTEEKLIETLTRAGKPMRSGEIAQATKLDKKEVEKAIKVLVKQ
                     GKLFSPVRCFYDIKK"
     gene            complement(898..1389)
                     /locus_tag="PK976_05900"
     CDS             complement(898..1389)
                     /locus_tag="PK976_05900"
                     /inference="COORDINATES: protein motif:HMM:NF025268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRC67454.1"
                     /translation="MNRLNLLFFIFLVLSIPSYSQHRVQIYHPEANALADLQQAIQKA
                     AKENKHVFVQIGGNWCPWCIKFHSFIHENAKVDSLLKADYIICLVNYSKENKNQEVLK
                     LLEYPQRFGFPVFLILDKNGQRIHTQDSGLLESGEGYDEGKVIHFLRNWSPRAFDPSL
                     YTL"
BASE COUNT          531 a          285 c          281 g          487 t
ORIGIN      
        1 tctaaagcat cgaagccaca aaatttgaga aagggaccga aaaaacctcc cacattgctg
       61 tccattacaa aattcgtagg cgaaatgctt accaccagtg atttccccgt acctgagtac
      121 tgtgtaatac ccgcaattgg gccaggtgaa ataacgactt cattttcagg atcattccat
      181 tttgtggtag gtaaagtgcc ttcccataag agttttaaac caaatccctt accaccggta
      241 aatttttctt tcatctcctc gtttacaggc ttttccttga attcgagagt atctacattg
      301 atatacaaag tgcgattgtt gtaacctcta taaatgggat gccattcgtg aacatattcc
      361 ttcaataatc tgtgcgtttt ccgaaaatct tcaatattca taactcgata tttttttaaa
      421 gaacttttat cctaaaaatt ttacaaatat ataagcaatt ttcctgtttt aaaatagaat
      481 aattaattaa agcatttatt tttttgaaaa attataattt aaaatatggc atagtgaact
      541 aaaacagtta gttataaatt tcgatttgaa aaatttttca acttgctttt tttaatttta
      601 tcattgaaaa acctgattcg acttaacata ttttatttcg aaagaacttt cagctttaaa
      661 accataaaaa cttatacctt tgcattaaac aataaataag aagactatat ggatacagag
      721 gaaaaactca ttgaaacctt aactcgagcg ggaaaaccca tgcgttctgg tgaaattgcc
      781 caggctacca aactcgataa aaaagaagta gaaaaggcca tcaaggtatt ggtgaagcaa
      841 ggcaagctat tctctccggt tcgttgcttc tatgatataa agaaatagaa tcatcgacta
      901 aagagtatag agagaaggat cgaatgcccg cggcgaccag tttcggagaa aatggatgac
      961 cttcccctca tcataacctt cacccgattc taaaaggccg gagtcttgcg tatgaattct
     1021 ctgaccattc ttgtcgagaa tcaagaacac cggaaatccg aaacgctgag ggtattcaag
     1081 aagctttaat acttcctgat ttttgttttc cttgctgtaa ttcaccagac aaattatgta
     1141 atcagccttc agaagtgagt caaccttggc attttcatga ataaagctat gaaattttat
     1201 acaccatgga caccagtttc caccaatttg aacaaaaaca tgtttattct ctttggctgc
     1261 cttttgaata gcttgctgaa gatcggccag ggcattggcc tcgggatgat atatctgaac
     1321 tctatgttgg gaataagatg ggatagacaa tactaaaaaa ataaaaaata acaggttcaa
     1381 tctgttcatg cgtaagattt ttcgagtttg ttattggcca caaaattagg acttcggttt
     1441 gaacgaagag atgatgcaaa ggaatgcaat tcatgcaatg aagaattcaa ttgttttttc
     1501 gaatgaaaat ggatgtaagt caacagatta gtaaatactt aaagaaagcc agagtcggga
     1561 ttgctggcgt aggtggctta ggtt
//
LOCUS       DAORPD010000462         1578 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_15277_length_1578_cov_2.115561, whole genome shotgun sequence.
ACCESSION   DAORPD010000462 DAORPD010000000
VERSION     DAORPD010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15277_length_1578_cov_2.115561"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="PK976_05905"
     CDS             complement(<1..527)
                     /locus_tag="PK976_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRC67455.1"
                     /translation="MITVSHISKTFGKTKVLSDISAQFETGRVHQVIGQSGSGKTVLM
                     KCLVGLLEVDEGEVLYDGRNFSQMTANQRKEIRKEMGMLFHGGALFDSMNVAENVAFP
                     MLMHSNLTTAEMKKRVDFCLERVNLAHSHKLIPSELSGGMTKRVAIARAIALNPHYLF
                     CDEPNSGLDPQTANM"
     gene            complement(524..1273)
                     /locus_tag="PK976_05910"
     CDS             complement(524..1273)
                     /locus_tag="PK976_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRC67456.1"
                     /translation="MRFIRIIEDIGHYVHLMKRVLTKPDKSLVLKRQFFVNVQSLGFD
                     SIGIVAIISLFIGAVATIQVAYNIDNPLIPLSLIGFTARQIIILEFSPTVVSLILAGK
                     IGSRIASEIGTMRVTQQIDALDVMGINSANYLILPKILASVFFNPILIVMSIAISLVG
                     GWIVSLTTSLLTPHDYIEGIRLFFDGFTVFYALIKTVVFAFIIASVSGFHGYRITGGA
                     HEVGQASTRAVVDSSIAIIISNLILTQLLLT"
BASE COUNT          483 a          304 c          371 g          420 t
ORIGIN      
        1 atcatgttgg cggtttgggg gtcgagaccc gagttaggtt catcgcaaaa aaggtaatga
       61 gggttaaggg ctatagcgcg cgcaatggcc actcttttgg tcattcctcc acttagttcg
      121 gatggtatta atttatgaga atgagcgaga ttgactcgtt ccagacagaa atccactctc
      181 tttttcattt cggcagtcgt cagattggaa tgcataagca ttgggaaggc cacattttcg
      241 gctacattca tcgaatcaaa cagggctcca ccatggaaca acatccccat ttccttcctg
      301 atttcttttc tctggtttgc tgtcatttgc gaaaaatttc ttccatcata caatacttca
      361 ccttcatcta cttcgagcaa gcccactaag cacttcatca aaaccgtctt gcccgatcca
      421 ctctggccaa taacctgatg aacccgcccg gtttcgaact gggcagaaat atcactcaat
      481 accttggttt taccaaatgt tttcgaaata tgagagactg tgatcatgta aggagaagtt
      541 gggtaagaat caaattggaa ataataatgg caatgctcga atcaactact gctctcgtac
      601 ttgcctgccc cacctcatgt gcacctcctg tgattctgta accgtgaaaa cccgaaacac
      661 tcgcaatgat aaaggcaaat accactgtct taattaatgc ataaaaaact gtaaaaccat
      721 cgaaaaaaag acgtattcct tctatgtaat cgtgaggagt aagaagagaa gtagtaagag
      781 atacaatcca gccaccaacc agacttatgg ctatgctcat gacaatgagg ataggattga
      841 aaaaaacgct ggccagtatc ttaggcaaaa tcaaataatt ggctgagttg atacccatta
      901 catcgagcgc atcgatttgt tgtgtaaccc gcatggtgcc aatttcggaa gcaattcgtg
      961 aaccaatttt cccggccagg ataagactga ctacagtggg agaaaactct aaaataatga
     1021 tttgccgtgc cgtgaaaccg atcaatgata atggaatcaa tggattatcg atattataag
     1081 ctacctgaat agtggctact gctccgataa acagactgat gatagctact ataccaatag
     1141 aatcgaatcc taatgattga acattgacaa agaactgacg cttaagtacc aatgatttat
     1201 cgggtttggt gagaaccctc ttcatcaagt gaacataatg ccctatgtct tcgatgattc
     1261 tgatgaatcg catgccaccc agctttggtg cgaaattacg ctttttctcg attgtcggaa
     1321 tgcaataatt tcgtataaaa atgaaaatgg gggatggtga atggagatta gtggtgtggt
     1381 gatcagggga cggggagagg ggggactggt ggataatgat tagtgattag aatttggaag
     1441 tggaaacgga ggaagggaga ctgcgggacg atgagaacgg gagattggtg gatagtggat
     1501 agtggatagt gaatggaaat tttgagagtg ggggacgaat agacgagggg actggaattt
     1561 gtaatttgga gattaaag
//
LOCUS       DAORPD010000463         1576 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_15306_length_1576_cov_2.269560, whole genome shotgun sequence.
ACCESSION   DAORPD010000463 DAORPD010000000
VERSION     DAORPD010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15306_length_1576_cov_2.269560"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1548)
                     /locus_tag="PK976_05915"
     CDS             complement(<1..1548)
                     /locus_tag="PK976_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
                     /protein_id="HRC67457.1"
                     /translation="MRKIANLLFVFLIFITFGLKAQEEGRLMRFPTIHGNQIVFSYAG
                     DLYTVDKSGGIARKLTSDANGYEMFARFSPNGKWIAFTGQYDGNTEVYVIPAEGGVPR
                     RLTYTATLQRDDISDRMGPNNIVMTWRDNESIVYRSRKQSFNDFKGQLFVVNIQGSLS
                     QEVPLPSGGFCSYSPDKSQLAFNQVFREFRTWKYYKGGMADDVWIYDFKTKKTQNITN
                     NIHQDIFPMWKDDFIYFLSDRDRTMNLFCYNIKTSQTEKLTNYTEFDIKFPSLGDDAI
                     IYENGGYIYVFDLKTRRAQRVNILLGDDFLLSRDTWKDASKSINSWDMMPDGKRAFFD
                     ARGDIFSVPAKEGVTFNLTSTSAIHERDIACSPDGKYIAYISDRNGEDEIYIQPADGS
                     GTAEQLTHLGDTYKYSMLWSPDSKKILWSDKLQRLQYIDVNTKKITLVDQTLEGEFNS
                     FTWSPDNRWIAYTLPSTRTSSRIFVYEVATGEKHFATDRWYDATSPAFSPDGKYLYFA
                     SNREFNPI"
BASE COUNT          465 a          316 c          322 g          473 t
ORIGIN      
        1 aatgggattg aactcacggt tggaagcgaa atacagatat ttcccgtcag ggctgaaagc
       61 tggagaggtg gcgtcgtacc aacgatcagt tgcaaaatgt ttttcgcccg tagcaacttc
      121 ataaacaaaa atacgagacg atgtgcgagt agaaggtaaa gtataggcaa tccagcgatt
      181 atcgggtgac catgtaaatg aattgaattc accttcaagg gtctggtcaa ccagcgtaat
      241 cttctttgta tttacatcga tgtattgcaa tcgctgcagt ttatccgacc ataaaatctt
      301 tttgctgtcg ggtgaccaaa gcatagaata tttataagta tcccctaaat gtgtaagttg
      361 ttcagctgtt ccacttccat cggccggttg aatatatatc tcgtcttcac cgtttctatc
      421 actgatataa gcgatgtatt tcccatcggg agagcaagca atatcacgtt cgtgaattgc
      481 cgaggtagaa gtaaggttga atgttacacc ttctttggca ggaacagaaa atatatcacc
      541 acgagcatcg aaaaatgctc tctttccgtc gggcatcatg tcccatgagt tgatgctttt
      601 cgaagcatct ttccaagtat cacgacttaa aagaaaatca tctcccagaa gaatatttac
      661 cctttgtgcc cggcgggttt tcaagtcaaa aacataaata tatcctccat tttcgtatat
      721 gatggcatcg tcgccaagac ttggaaattt aatatcaaat tccgtataat ttgtcaattt
      781 ctcggtttga ctggttttta tgttatagca gaaaagattc atagttctgt ctctatcgga
      841 gaggaaataa ataaaatcat ctttccacat ggggaagata tcctgatgga tattattagt
      901 aatattttga gtttttttag ttttaaagtc ataaatccag acatcatcgg ccatgccgcc
      961 cttataatat ttccaagttc tgaattctct aaatacctga ttgaaggcta attgtgactt
     1021 atcgggagag taggagcaaa agccacccga gggcagtgga acttcctggc tcaggcttcc
     1081 ttgaatattt accacgaaaa gctgtccttt aaaatcgttg aagctttgtt tgcgtgagcg
     1141 atatacaatg gattcgttat cgcgccatgt cattacgatg ttatttggcc ccatacggtc
     1201 agagatatca tcgcgctgta aagttgccgt ataagtcaag cgacggggaa cgccaccttc
     1261 ggcagggata acgtaaactt cggtatttcc gtcgtattgt ccggtaaagg cgatccattt
     1321 cccattgggt gaaaagcgtg caaacatttc atagccatta gcatcggaag taagtttacg
     1381 ggcaataccc ccagatttat ctacagtata aaggtcgcct gcatagctaa aaacaatctg
     1441 atttccatga atggttggaa atctcattaa tcttccttct tcctgagctt tcaatccaaa
     1501 tgtgatgaat atcaggaaaa cgaacaacag gttagcaatt tttctcattt caaaagcttt
     1561 aaaaatttga aggcat
//
LOCUS       DAORPD010000464         1574 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_15347_length_1574_cov_2.530612, whole genome shotgun sequence.
ACCESSION   DAORPD010000464 DAORPD010000000
VERSION     DAORPD010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15347_length_1574_cov_2.530612"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1559)
                     /locus_tag="PK976_05920"
     CDS             complement(<1..1559)
                     /locus_tag="PK976_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67458.1"
                     /translation="MITFTYGTNGPYSMLPADVDGTLPPEDEPAYFAYKSDPNQLVIY
                     KMNIDWTNPTASTFGLDQSILVAFYSSNISGIPQPGTTRKLDPITDRLMFRLQYRNFG
                     SYASMVTSHTVNVSGTAGVRWYEVRKTPTTSWSLYQQGTYAPDNVHRWMGSLAMDSFG
                     NIALGYSVSSSTVYPGIRYTGRLVNDPLGEMTIQETTIIDGGGSQTGGFMGNGRWGDY
                     SAMMVDPVNPSTFWYTQEYMQTTSSQGWKTRIASFSFENVFAVTLTASPDHICLGDSS
                     HLFALAMGGTGNYTYSWTSYPEGFISNLPNPWVSSIDTTIYICQVTSGNDTITDSVSV
                     FVNLPPVVWAGNDTVICANNSLQLDALAVNYDSLLWITSGDGTFSANNTLTSVYTPGA
                     GDIASGSVNLGLYATALTGCPGPAFDEIILNITPLPVVSAGTDTLICFWQLPFGCMGQ
                     STNTTSIYWTTYGDGTFDDPENLNAKYFAGSNDMNNGSVILKLTGQPLIPCTDNVSDE
                     MTLYLDPCVGIV"
BASE COUNT          464 a          324 c          376 g          410 t
ORIGIN      
        1 actataccca cgcaagggtc gagataaaga gtcatttcat cggatacatt gtcagtacaa
       61 ggaatcaggg gttgaccagt aagttttagg ataacgcttc cattgttcat atcattagag
      121 ccggcaaaat atttggcatt caagttttcg ggatcatcaa aggttccatc tccataagta
      181 gtccaataaa tcgaggtggt gttggtggat tgacccatac agccaaaagg aagttgccag
      241 aagcagatga gagtatcggt tcctgctgaa acaacaggca aaggagtaat attcaggatt
      301 atttcatcga aagctggacc ggggcagcct gtaagagccg ttgcataaag gccgagatta
      361 acgcttccgc tggcaatatc gcctgcaccg ggggtataga cagatgtaag tgtattatta
      421 gccgaaaaag tcccatctcc agaggttatc caaagcaggg aatcgtagtt tacggccaaa
      481 gcatcgagtt gaagactgtt gttagcgcat attactgtat cgttcccggc ccaaaccaca
      541 ggtggaaggt tcacaaaaac agaaacactg tcggtgattg tatcatttcc acttgttacc
      601 tggcaaatat atattgttgt gtcaatggaa cttacccagg gattaggaag attggaaatg
      661 aatccttcgg gataagatgt ccatgaataa gtataattac ccgttcctcc cattgctaaa
      721 gcaaacaaat gagatgaatc gcccaagcag atatgatcgg gggaagcggt gagtgtaacg
      781 gcaaatacat tttcgaagga aaaggaggca atacgtgttt tccagccttg agaactggtt
      841 gtctgcatat attcctgagt gtaccagaat gtagaaggat taacgggatc gaccatcatg
      901 gctgagtaat cgccccaacg gccattcccc atgaatcctc cagtttggga acctccaccg
      961 tcaatgattg tagtttcctg aatagtcatt tctcccaaag gatcattcac caagcgtcca
     1021 gtataacgta ttcccggata gacggtagaa ctggatacag aataacctaa ggcaatgttt
     1081 ccgaaagaat ccatggcaag agagcccatc cagcgatgta cattgtcggg cgcataagtt
     1141 ccttgctgat agagcgacca tgaggttgtg ggggtctttc ttacttcata ccatcttact
     1201 cccgccgtac cacttacatt aaccgtatgg ctggtaacca tgctggcata cgatccaaaa
     1261 tttctgtact gtaacctaaa catcaggcga tcggtaatag ggtcgagttt gcgagtggta
     1321 cctggctgtg gtattcccga aatattcgaa ctataaaatg caacaagtat ggattggtct
     1381 aaaccaaaag tcgaggcagt tggattagtc cagtcaatat tcattttata gattactaac
     1441 tggttgggat cgcttttata agcaaaatag gcgggttcat cttcgggagg taaagttcca
     1501 tcaacgtcgg caggaagcat gctataaggg ccatttgttc cgtaagtgaa ggtaatcatt
     1561 gttgcattgg gatc
//
LOCUS       DAORPD010000465         1574 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_15350_length_1574_cov_2.155365, whole genome shotgun sequence.
ACCESSION   DAORPD010000465 DAORPD010000000
VERSION     DAORPD010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15350_length_1574_cov_2.155365"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1574
                     /locus_tag="PK976_05925"
     CDS             <1..>1574
                     /locus_tag="PK976_05925"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRC67459.1"
                     /translation="ANNTQYIELINEKNKLQAEAAGQSFTPLDPANFPDYTDWYDEVM
                     RDQAFVMNHDIGICGGTENSAFYFGAGYFKQDGLLLNQTYERLNIRSSLDSKVNDALK
                     VGYSSTFSTFRTNDAPSVFYDAYVAPPVFKPKVNDTTFTNPVDLGLGNFSNPAASLYY
                     YNSKSNGIRLVGSVFAEVNFLKYFQFRSQYGTDLGYARNRNYTPYYWVSPTQKDTNRT
                     LSRQWTYDYKGVWDNTLTFEDNFGPHRLKVMAGMSAQQENTLGTYGSRMGVPYLGENT
                     LYINLGRAKTSQVNDWGSKITSLSYFGRVNYSLYDRYLLTTTLRYDGSSVFPKDDRWD
                     IFPSVGVGWIITGEEFMKDQNIFDFLKLRASWGVIGNNKIPANVYTLSVEEGGILNTA
                     FGSGQNGNTVIVEGANITSAVPPQLKWEKMNEFDIAIEGKSLDSRLSYEIDFYSRTTK
                     DAIFLLTLSATAGTSGSYYSNNADILNQGIEITLGWADKVGKFGYDLNLNYAYNHNEV
                     KKLREGTLGLFGGNLP"
BASE COUNT          463 a          333 c          338 g          440 t
ORIGIN      
        1 agctaacaat acacaatata tagaactaat caacgagaag aacaagttgc aggcggaggc
       61 agccggccaa tcattcaccc cactggatcc tgccaatttc cccgattaca ctgactggta
      121 tgatgaagta atgcgtgatc aggcctttgt aatgaaccat gatataggga tctgtggtgg
      181 aaccgaaaac agcgcatttt atttcggagc cggatatttc aaacaagatg gcttactctt
      241 gaatcaaacc tatgaaaggc tgaatattcg ttcttcatta gattcgaaag taaatgatgc
      301 cctcaaggtt ggatactctt ccaccttctc tacctttcgc accaatgacg ccccttcggt
      361 attctatgat gcttatgtgg ctcctccggt attcaagcca aaagtaaacg ataccacctt
      421 caccaatcct gttgatctgg gtctgggaaa ttttagcaac ccagccgcca gtctatatta
      481 ctataatagt aaaagcaatg gtataaggtt ggtaggaagt gtgtttgctg aagttaattt
      541 tctgaaatac ttccaattcc gttcgcaata tgggaccgac ctgggatatg ctcgtaaccg
      601 caattatacg ccctattatt gggtttctcc cactcagaag gatactaatc gtactttaag
      661 caggcaatgg acttacgatt acaaaggggt ttgggataac accctcacct ttgaagacaa
      721 ttttggacct catcgcttaa aggtaatggc aggcatgtcg gctcagcaag aaaatactct
      781 gggcacttat ggttctcgca tgggtgttcc ctatcttgga gaaaatacac tctatatcaa
      841 tcttggacgg gctaaaacca gtcaggtaaa cgactgggga agcaaaataa cttccctctc
      901 gtattttggc cgtgtaaatt attcacttta tgatcgttat ctgctaacta ccactcttcg
      961 ttacgatggc agttcggtat tccccaaaga tgacaggtgg gatattttcc cctctgtggg
     1021 tgtgggatgg atcataacgg gtgaagaatt catgaaggat cagaatatat tcgacttctt
     1081 gaagctaaga gccagttggg gtgtaattgg gaataacaag atccctgcca acgtctatac
     1141 cttatccgta gaagaaggtg gcattttgaa taccgctttt ggttcaggtc aaaatggaaa
     1201 taccgtcatt gttgaaggag ccaacatcac aagcgcagta cctcctcaac taaaatggga
     1261 gaagatgaat gaattcgata tagccatcga agggaaatca ttggatagtc gactttcgta
     1321 tgaaatcgat ttttattcga gaaccactaa ggatgctatt ttcctgctta ccctatcggc
     1381 tactgcaggt acttcgggtt catactactc caataatgcc gacattctta accagggaat
     1441 cgaaatcacc ttgggatggg cagataaagt cggaaaattt ggctatgacc tcaatttaaa
     1501 ctatgcatac aatcataatg aagtaaaaaa acttcgtgaa ggcacgctcg gtttatttgg
     1561 aggcaattta cctg
//
LOCUS       DAORPD010000466         1573 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_15370_length_1573_cov_2.254941, whole genome shotgun sequence.
ACCESSION   DAORPD010000466 DAORPD010000000
VERSION     DAORPD010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15370_length_1573_cov_2.254941"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1573
                     /locus_tag="PK976_05930"
     CDS             <1..>1573
                     /locus_tag="PK976_05930"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRC67460.1"
                     /translation="RGGDGMECIIDYQEPAYSYASLYYGDIARFYNNNGGYTIAAKGK
                     FGITEDGDWITPYTLHVTDPNRMFIGYKNIWRGQYIRTTPQWVKISDNLAGNNNTNIR
                     IVEQSQANPDILYFARWDNKLFRTDNANAGQPVWVNLTSLLPLSGQTPSALRPHPTKP
                     DIIYMALGGKIYKSQNRGQSWTDISGSLPNVEYSSIAFYIRAHEGLYVASDIGVFYKD
                     STLTDWIQFSNGLPVDASIREIEIAYNNQNNEYDRIRAGTYGRGVWSSSVYRTPPTAS
                     FTSDVIIVPVTGQVHFKDISSGIPSSWQWTFEGGQPATSTIQNPVITYNNPGSFKVKL
                     IVSNSLGIDSIEFQNYITVSNTILPLVGFYADNTIPCSGQIVRLYDTTLYGPISWHWQ
                     FQPNTIAYHNGTFYNSQNPVVQFNENTEYTITLTASNPNGTSSVTRQNYIHSGGYLMP
                     FYEDFETLNFADRSWTVENPDMGITWDMAQVESIHGPTVAARMQFFYYYLMNQRDRLI
                     SPPLNFSNENKVILS"
BASE COUNT          472 a          368 c          293 g          440 t
ORIGIN      
        1 caagaggcgg agatggaatg gaatgcatca tcgattatca agagccagcc tattcatacg
       61 cttcattata ttatggcgat attgcacgtt tttacaataa taatggaggt tacaccatag
      121 cagctaaagg taaatttgga attaccgaag atggcgactg gattactcct tacactttgc
      181 acgttactga tcccaacaga atgttcattg gatacaagaa catctggcgc ggtcaatata
      241 ttcgtactac cccacaatgg gttaaaattt ccgacaatct cgcaggtaac aacaatacaa
      301 atatccgtat agttgagcaa tcacaggcaa atcccgatat attatatttt gcccgctggg
      361 acaataagct cttccgtacc gacaatgcca acgcaggtca acccgtttgg gtgaatctta
      421 catcattatt acccctttca ggccagacac catctgcttt aaggcctcat cctaccaaac
      481 cagatatcat ttatatggcc ttgggtggta aaatctataa atcacaaaat cgcggacaaa
      541 gctggaccga catcagtggc agcttgccca acgtggaata ttcttccatt gccttttaca
      601 ttagggctca cgaaggactt tatgtagctt cagatatcgg ggtattttac aaggactcta
      661 ctctgaccga ctggatacaa ttcagcaatg gactcccagt ggatgcatcc attcgtgaga
      721 tcgaaattgc ctataacaat caaaataatg aatacgaccg cattagagca ggtacatatg
      781 gtcgaggcgt atggtcttcg agcgtttacc gtactccacc tacagcttct ttcacatccg
      841 atgtaatcat agtgcctgtt acaggccagg ttcacttcaa agatatttcg agtggcattc
      901 caagttcatg gcaatggact ttcgaaggag gtcaacccgc aacttctacc attcaaaatc
      961 cagtaataac ctacaacaac ccgggctctt tcaaagtaaa acttatcgta agcaattcat
     1021 taggaatcga ctctatcgaa tttcagaact atattaccgt aagtaatacc attttacctc
     1081 ttgttgggtt ttatgccgat aacactattc cttgttcggg acaaatcgtt cggctttacg
     1141 atactacact ctatgggcct atttcgtggc attggcaatt ccagccaaat accatagctt
     1201 atcacaatgg aactttctat aattcgcaaa accccgtggt tcaattcaac gaaaataccg
     1261 aatacaccat aacacttacg gcttccaatc cgaatggtac atcttccgta acgagacaaa
     1321 attatattca ctcaggaggt tatctcatgc ctttctatga agatttcgaa actttgaatt
     1381 ttgctgatcg ttcatggaca gtggaaaacc ccgatatggg aattacttgg gatatggctc
     1441 aggtcgaaag cattcacgga cccactgtgg ctgcccgaat gcagtttttc tactattatc
     1501 taatgaacca acgagaccgc ctgattagtc ctccactgaa tttctcgaat gaaaacaaag
     1561 taatcctatc gtt
//
LOCUS       DAORPD010000467         1572 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_15387_length_1572_cov_2.214898, whole genome shotgun sequence.
ACCESSION   DAORPD010000467 DAORPD010000000
VERSION     DAORPD010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15387_length_1572_cov_2.214898"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..797
                     /gene="miaA"
                     /locus_tag="PK976_05935"
     CDS             <1..797
                     /gene="miaA"
                     /locus_tag="PK976_05935"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HRC67461.1"
                     /translation="FYKELPIGSATPSEEQLAKVKHYFIGNRTIHHPCNVAEYEEEVI
                     ALLSRLFTSQPMVIMAGGSGLYIDAVCNGLDILPEPDLLLRHHLHALYHEQGIEALRT
                     QLRLLDTEFYHQVDLKNPIRLMRAIEVCMLSGQPYSSLRTGKSKLRSFHILKLALDVP
                     KPLLIERINHRVDEMMQRGLLEEAQRLLPFRNLQPLRSLGYKELFAYMEGQFTLEKAI
                     EKIKINTRKFAKRQITWFKKDPLYHWIPPNDLQHIDQLIENALQQS"
     gene            932..>1572
                     /locus_tag="PK976_05940"
     CDS             932..>1572
                     /locus_tag="PK976_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67462.1"
                     /translation="MKKIIINLLRHLTFWLLIFASGRIFFLIYYHGIIAHSHIKFTEI
                     AGLFFHALRLDVATFCYIASLFLFLYVIESITKWRAINQIVKYLNFLLIGIYFLIVAG
                     ETGLYDEWHTKLNMKALAYLKHPAEVIASAQTLTLVIMILFWLVSVFTACWLYNKFIF
                     QASTKQSSPKAWKAVITYIVLQPILIIGSRGGIQQIPINQSQSYFSKHAILNHA"
BASE COUNT          466 a          288 c          284 g          534 t
ORIGIN      
        1 agttttataa agaactccct ataggttcgg ctacgccttc tgaggaacaa ttggcaaaag
       61 ttaagcatta ttttattggc aaccggacta ttcatcatcc ttgtaatgta gctgaatatg
      121 aagaagaagt tatagctctt ttgagtagat tatttacatc ccagcctatg gtaatcatgg
      181 ctggaggttc gggtttatac atcgatgctg tttgcaatgg cctcgatatt ttgcctgaac
      241 ctgacctatt actccgccat catttacatg ctttgtacca cgaacagggg atcgaagccc
      301 tgagaaccca attacgcctc ctcgataccg aattctatca tcaggttgac ttgaaaaatc
      361 cgatacgatt gatgagagcg atagaagtgt gtatgttatc tggccagcct tattcgagcc
      421 ttcgtactgg aaaatcgaaa ttgcgtagtt ttcatatcct taagcttgcg cttgatgtac
      481 ccaagccttt attgatcgaa agaatcaatc atcgtgtcga tgaaatgatg caaagagggc
      541 tgctcgaaga agctcagaga ttgttaccct tcagaaatct tcagcctttg cgcagcctgg
      601 gttataagga attgtttgct tatatggaag gacaatttac tctggaaaaa gctatcgaaa
      661 aaataaaaat caatactcga aaattcgcta aaagacaaat cacttggttt aaaaaagacc
      721 cactctatca ttggattcct ccgaatgacc tccagcatat cgaccaattg atcgaaaatg
      781 cattgcaaca atcttgattt tttaccgaaa ataaattaaa aatcaatttt tttttatttt
      841 taaatgtgat ctccatatta taagtcgaaa aatatatttt ttgtgatttc tttttttata
      901 tttattcata tactttcaat ttgaatcaat tgtgaaaaaa ataatcatca atcttttacg
      961 tcatttaacc ttttggctgt tgatctttgc ttctggaaga atattttttc tgatttatta
     1021 tcatggaatt attgctcatt ctcacataaa atttacggag atagccggcc tgttctttca
     1081 tgctttgcgg cttgatgtgg ccacattttg ctatattgct tcattattcc tttttttata
     1141 tgtaattgaa agtataacta aatggagggc aattaatcaa attgtgaagt acttgaattt
     1201 tttattgatt ggaatttatt ttttgattgt tgccggtgaa acgggtttgt atgatgaatg
     1261 gcatacaaag ctcaacatga aggcgcttgc gtatctgaag catcctgcag aagtgatagc
     1321 ttcagcccaa acactgacgc tcgtgataat gatacttttc tggctagtat cggtttttac
     1381 tgcttgctgg ctatataata agtttatctt ccaggcttca acaaagcaat catctccaaa
     1441 ggcatggaag gcagttataa cttacattgt gttacaaccc attctcatta ttggttccag
     1501 aggtggcatt cagcaaatac ctatcaatca gagtcaaagc tatttttcga aacatgctat
     1561 cttgaatcat gc
//
LOCUS       DAORPD010000468         1572 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_15388_length_1572_cov_2.199736, whole genome shotgun sequence.
ACCESSION   DAORPD010000468 DAORPD010000000
VERSION     DAORPD010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15388_length_1572_cov_2.199736"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="PK976_05945"
     CDS             <1..425
                     /locus_tag="PK976_05945"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF014033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009235 - cobalamin metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP:cob(I)alamin adenosyltransferase"
                     /protein_id="HRC67463.1"
                     /translation="LVIDLCNDMPTKEFLMLIQQKLFAVESLFAAASSEFQKDLLGVY
                     ENDIEMLEREIDRMNEVLPQLNSFILPAGHPSISSAHVARTVCRRAERCMVRAFSGSE
                     QELAALKFINRLSDFFFVLARKFAFDLNIPEIPWPANK"
     gene            505..726
                     /locus_tag="PK976_05950"
     CDS             505..726
                     /locus_tag="PK976_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HRC67464.1"
                     /translation="MYWTLELASKLEDAPWPATKDELIDYAIRTGAPQEVIENLMEIE
                     DEGDVYESIEDIWPDYPTKEDFFFHEDEY"
     gene            874..>1572
                     /gene="secA"
                     /locus_tag="PK976_05955"
     CDS             874..>1572
                     /gene="secA"
                     /locus_tag="PK976_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540830.1"
                     /note="functions in protein export; can interact with
                     acidic membrane phospholipids and the SecYEG protein
                     complex; binds to preproteins; binds to ATP and undergoes
                     a conformational change to promote membrane insertion of
                     SecA/bound preprotein; ATP hydrolysis appears to drive
                     release of the preprotein from SecA and deinsertion of
                     SecA from the membrane; additional proteins SecD/F/YajC
                     aid SecA recycling; exists in an equilibrium between
                     monomers and dimers; may possibly form higher order
                     oligomers; in some organisms, there are paralogous
                     proteins that have been found to be nonessential but do
                     function in secretion of a subset of exported proteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HRC67465.1"
                     /translation="MLNFFKKFLGSKSERDIKAIMPIVNQIHVAYEEIQKLSNDELRA
                     KTKEFKTRIASYIAEEESKIAELKARIENEIDMDVEEKAEIYKQIDALSKLSYQKSQK
                     ILDDILPEAFAVMKDTARRFYENEQVIVTATQMDRDLAAHHENIRIEGDKAIYNKQWM
                     AGGNMITWDMVHYDVQLIGGIVLHQGKIAEMATGEGKTLVATLPVYLNALTGKGVHVV
                     TVNDYLAKRDSEWMG"
BASE COUNT          485 a          307 c          330 g          450 t
ORIGIN      
        1 gactcgtcat cgatctatgt aatgatatgc ctacaaaaga atttttgatg ctaatccagc
       61 agaaattatt tgcagtagaa agtctttttg ccgcagcttc atcagaattt caaaaggatc
      121 ttctcggggt ttacgaaaat gatatcgaaa tgcttgaaag agaaatcgac cgcatgaatg
      181 aagttttgcc tcaattgaat tcatttattc tacccgcagg gcatccttcg atttcgtctg
      241 cccatgtggc acgtacagtt tgtcgccgag ccgaacgttg catggtaaga gcgttttcgg
      301 gcagcgagca ggagctcgcg gctttgaagt tcatcaatag attatcggat ttctttttcg
      361 tacttgcccg caaatttgct ttcgatctga atatacccga aattccctgg cctgctaata
      421 aataattttt tcgaatttta cttatagaaa caaagtttgt tatatttttg caaacttttt
      481 tagttataaa taaattattt cattatgtac tggacgcttg aactggcatc gaaattagag
      541 gatgctccct ggcctgccac caaggacgaa ttgattgatt acgctattcg gacaggcgct
      601 ccacaagagg ttatcgagaa tctcatggaa attgaggacg aaggcgatgt ttatgaaagc
      661 atagaagata tttggcctga ctaccctact aaagaagact ttttctttca tgaagacgag
      721 tactaatttt tttcgaccca tctacatggc agggttctac cctgcttttt tcatcttcat
      781 aacgcctgca ttcccctcta tgtgtagatg attttggcta actttgcaaa ttatcttttt
      841 ataatccatt gaattattat caactaaata aaaatgttaa acttcttcaa gaaattcctc
      901 ggttctaaat ccgagcgcga tattaaagcc ataatgccta tcgtaaatca gattcatgtg
      961 gcttatgaag aaattcaaaa attatctaac gatgaactga gagccaagac aaaagaattt
     1021 aaaaccagga tcgccagcta tattgccgaa gaagaatcga aaattgcaga actgaaggct
     1081 cgcatagaga acgaaattga catggatgta gaagagaaag ccgagattta taagcaaata
     1141 gatgctcttt cgaaactttc ctatcaaaaa agccagaaaa ttctggatga tatcttgccc
     1201 gaagcctttg ctgtgatgaa agataccgcc agaagattct acgaaaacga acaggtaatc
     1261 gtcactgcca cacaaatgga tcgcgatctg gcagctcatc acgaaaatat tcgaatcgag
     1321 ggagacaagg ccatctacaa caagcaatgg atggccggcg gaaacatgat cacttgggac
     1381 atggtgcatt acgatgtaca actcatcgga ggtatagttt tgcatcaggg aaagattgcc
     1441 gaaatggcta ctggcgaagg aaaaaccctt gtagctaccc tgccggtcta tctgaatgcg
     1501 cttacaggta agggggttca tgtggttacg gtaaatgatt atttggccaa acgcgattcg
     1561 gaatggatgg gt
//
LOCUS       DAORPD010000469         1570 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_15412_length_1570_cov_2.931353, whole genome shotgun sequence.
ACCESSION   DAORPD010000469 DAORPD010000000
VERSION     DAORPD010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15412_length_1570_cov_2.931353"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1570)
                     /locus_tag="PK976_05960"
     CDS             complement(<1..>1570)
                     /locus_tag="PK976_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67466.1"
                     /translation="LMFQERYLYDLDDFNNSLYERHDLSWIKSAWIILLQSAWDHEWY
                     DALIHQSIYEETLSKYDRMVGGYDVFAIWPTWPRLGLDQRSQFELYEDLPGGLKQLKH
                     QAELAHEMGKKYFLSYNPWDKPEQEKFHRTNLKKLLEQTDADGLVLDTKGESDAWLQN
                     LADSVKPGIIMYSEGMAVPKNMTGIVAGRVHDALYMPPVLNLNKLIKPDFAIFRVLQV
                     ADGELHREIAAAFFNGYGVEINTMRPGRPASLQDDLFFLGQTTKILRDNQSVFFNYNY
                     LPLIHTQMDSIYVNKWETDEKIIYTIYSLRPTGFRSPLFGFDELQVTNSSITEEPYHF
                     VSLWNHEEIQAVKMDGKYYLPVNVASFDRAFLGTRSEAQVECIAVLPKLLEVDLEHDT
                     LYFGSSFGNRIEITAGNPSYSSRTFPFDMGKGFISLYETFGRYEGKIVIQLYGGNELL
                     DERIVKIDPGSPRLIVRQEQTNKPAIAPEDMIFIPGGRYDFVSYDDEDDDEPFISCPY
                     HNDTLHLIMKSFYM"
BASE COUNT          444 a          346 c          327 g          453 t
ORIGIN      
        1 tccatataaa aggatttcat gattaaatga agagtgtcat tgtggtaagg acaagaaata
       61 aaaggttcat catcatcttc atcatcatag gatacaaaat catatctccc tcccggaatg
      121 aaaatcatat cttccggagc aatggctggt ttattggttt gttcttggcg aacaatcaat
      181 cttggacttc ctgggtcgat tttcacaatc ctctcgtcta ataattcatt ccctccatag
      241 agctggatta cgattttccc ttcgtaacgc ccgaaggttt catagagtga aataaaacct
      301 ttccccatgt cgaaagggaa ggtcctgctc gaataagatg gattcccggc agtaatctcg
      361 atgcgatttc cgaaggaaga accaaaatac aaagtatcgt gttccaggtc gacttccaaa
      421 agcttaggca aaacggctat gcattctact tgagcctcac ttcgggttcc caaaaaggcc
      481 cgatcgaacg aagccacatt cacaggtaaa taatatttcc catccatttt tacggcttga
      541 atttcttcgt gattccaaag gctgacaaaa tgataaggtt cttctgtgat gcttgaattg
      601 gtgacctgca attcgtcgaa tccgaataag gggctacgga acccagtagg gcgtaaactg
      661 tagatggtat atatgatttt ttcgtcggtt tcccatttgt tgacatagat gctatccatc
      721 tgtgtatgaa tcaggggtaa ataattataa ttgaagaata cactctggtt gtctctcaga
      781 attttggtag tttgacccag aaagaagagg tcatcttgca gagaagcagg cctgcctgga
      841 cgcatggtgt tgatttctac tccatatcca ttaaaaaaag ccgcagcaat ttcgcgatgt
      901 aactctccgt cggcaacttg cagaacacga aatatggcaa aatcgggttt gatgagttta
      961 ttcagattca agacgggagg catatagagt gcatcgtgaa ctcttccggc aactattccc
     1021 gtcatattct tcggaacggc cattccttcc gaatacatga taatccctgg ctttacgctg
     1081 tcggcaagat tctgcaacca ggcgtccgac tcgccctttg tgtcgagtac caaaccatcg
     1141 gcatcggttt gttcgagcaa cttcttcaga ttggtcctgt gaaatttttc ctgttcaggt
     1201 ttatcccacg gattgtaaga aaggaagtat ttcttcccca tttcgtgagc gagttctgct
     1261 tgatgtttga gttgtttcaa tccaccagga agatcttcat agagctcgaa ttgtgatctc
     1321 tggtcgagtc caaggcgagg ccatgtaggc catatcgcaa aaacatcata gccacccacc
     1381 atccgatcgt atttcgaaag tgtttcttca taaatagatt gatggatcaa cgcatcatac
     1441 cattcgtgat cccaggccga ctgaagcagt ataatccatg cagattttat ccaagataga
     1501 tcatgacgct catagagcga attattaaag tcatccagat cgtacagata acgttcctga
     1561 aacatcaatt
//
LOCUS       DAORPD010000470         1570 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_15416_length_1570_cov_2.264686, whole genome shotgun sequence.
ACCESSION   DAORPD010000470 DAORPD010000000
VERSION     DAORPD010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15416_length_1570_cov_2.264686"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1570
                     /locus_tag="PK976_05965"
     CDS             <1..>1570
                     /locus_tag="PK976_05965"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HRC67467.1"
                     /translation="SLIYDDRLLNTLVKAEGINSELSGDLTAESTSLQTFTLIKDIMV
                     DYGGIRFLNKTECELKAEVDANLKDMIFTMKDNEVRINQLFLGFDGKVQIMEEGYGLD
                     LSFSSRQTSFKNLLSMVPSIYAKDFEKLTASGTMKFHGKIKGIYADTSFPSYDIVLEV
                     NEGRFQYPSLPSAVENVAIRANVVNPDGQPNNLTLDLSRFHLEMAHNPIDLKVLLKNP
                     ISDPWIKAVILGRLDLAEIKNVYPLGINLKGSIRADLSLEGNISTLEKGNFENFDAKG
                     SLVIDDLFLPDTSTKKDLNISTARLNFSPAYLELANCKINIGANDIAANGKVTNYLGY
                     LLRNTDLKANLNVSSNRLNLDEFVTSGSKSQNDKTQLGIIEVPKRIDFALNANLNQVV
                     FGKLEMNDIRGEIQVKNQQAFLDNLSLKTLGGLITLKGSYNTINPTHPCMELNLKIEN
                     IDIPLTASTFVTVQKITPILENVKGLASASLSLKTDLDPNMIPDLNTLSSSGTIQTGN
                     LKISNVAALQKLDDI"
BASE COUNT          517 a          322 c          299 g          432 t
ORIGIN      
        1 cagtttaatc tatgatgacc gcctgctgaa tactttggtc aaagcagaag gaataaatag
       61 tgagctttcg ggtgatttga ctgccgaaag cacttcatta caaactttta ccctgattaa
      121 agacattatg gtagattatg gcggtatacg tttcctaaat aaaacagaat gcgaattgaa
      181 ggcagaagtt gacgccaatc tcaaggacat gatttttaca atgaaagata atgaagttcg
      241 aataaatcaa ctgtttttag gattcgatgg taaagttcag attatggaag aagggtatgg
      301 cctcgacctc agtttttcga gccggcaaac gagtttcaaa aacctgcttt cgatggtgcc
      361 ttctatttat gccaaggatt ttgaaaaatt gaccgcttcg ggcacaatga aatttcatgg
      421 aaaaataaaa ggaatttacg ctgatacatc attcccctct tatgatattg tactcgaggt
      481 aaatgaaggc agatttcaat accccagctt gccttctgct gttgaaaacg tagctattcg
      541 agccaatgtg gtcaatcccg acgggcaacc caacaacctg actctcgatc tcagccgttt
      601 tcaccttgaa atggctcata accctatcga cctgaaagtt ctactaaaaa accctatcag
      661 cgatccatgg ataaaagccg tcatcttagg aagactcgat ctggcagaaa taaagaatgt
      721 ttatccactt ggcatcaatc ttaaaggtag tattagagcc gatctatcgc tcgaaggaaa
      781 tatttctact ctcgaaaaag gaaacttcga aaatttcgac gctaaaggca gtttggtaat
      841 agatgattta ttcctacccg atacgtctac caaaaaagat ctcaatatct caacggcacg
      901 gcttaacttt tcacccgcct atctggagct tgccaactgt aagataaata ttggagctaa
      961 cgacattgcc gcaaatggaa aggtaaccaa ctatctggga tacctccttc gaaataccga
     1021 cttgaaagcc aatctgaacg tcagttcaaa tcgccttaac cttgatgaat tcgtgacttc
     1081 aggctcaaaa agtcagaatg ataaaaccca attaggaatc atagaagtgc caaaacgcat
     1141 tgactttgca ttgaatgcca atctgaatca agtcgtgttc ggtaaactcg aaatgaatga
     1201 tataagaggt gagattcagg ttaaaaatca acaggctttt ctggataatc tttctttaaa
     1261 aacattggga ggcctcatta cattgaaggg ttcttacaat acgataaatc ctacccatcc
     1321 atgtatggaa ttgaacctca agatagaaaa tatcgatatt ccactcacgg cttcgacttt
     1381 tgtgactgtg cagaaaatca cccctatact cgaaaatgtg aagggattag ccagtgcttc
     1441 actcagtttg aaaaccgatt tagaccccaa tatgattcct gatttgaata cactttcttc
     1501 gagtggaacc attcaaacag gaaacctaaa aatttcgaac gttgcagctt tgcaaaaatt
     1561 agatgacata
//
LOCUS       DAORPD010000471         1568 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_15455_length_1568_cov_1.907469, whole genome shotgun sequence.
ACCESSION   DAORPD010000471 DAORPD010000000
VERSION     DAORPD010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15455_length_1568_cov_1.907469"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="PK976_05970"
     CDS             complement(<1..634)
                     /locus_tag="PK976_05970"
                     /inference="COORDINATES: protein motif:HMM:NF018899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HRC67468.1"
                     /translation="MKSNRFILPIAFTLFIYFVPFYVQAQITIGSPQVSIDYDNPKEY
                     EIGGITITGVQYLDASVLTMLTGLKIGDRIKVPGEEITLAIQKLWRQGLFDNVRIIAQ
                     RIEDNLIFLEIQLVERPRLSKFTFIGIKKGEADDLRDKLKLASGEVVTRSSLVRAEET
                     IKKYFTDKGFLNVQVDIQEIRDTTKLNNVILKFTIDKKQKVKINEIIIHGN"
     gene            complement(678..1442)
                     /locus_tag="PK976_05975"
     CDS             complement(678..1442)
                     /locus_tag="PK976_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HRC67469.1"
                     /translation="MSETSRLQEIKASLDMGKLPRHIAIIMDGNGRWARQRGMDRVMG
                     HQHAITAVRETAEAAAELGIRYLTLYTFSTENWKRPQCEIDAIMSLLVETIHNETDTL
                     TKNNIRLRAIGDLDQLEKSTRQALENAIEITSSFTRMDLVLAISYSARWEITEMVKKI
                     AREVENGTLLPSQITQEIISAYLNAPFLPDPELLIRTSGEYRISNFLLWQIAYTELYF
                     TPVLWPDFRKEDLFLAIADYQRRERRFGKISEQLSS"
BASE COUNT          420 a          294 c          334 g          520 t
ORIGIN      
        1 ggtttccatg aatgataatt tcgttgattt taaccttttg ttttttatcg atagtaaatt
       61 tcagaatgac attattgagt tttgtggtat ctctaatttc ttggatatca acctgaacat
      121 tcaaaaatcc tttatcggta aaatattttt taatagtttc ttcggctctg acgaggctgc
      181 tgcgggtaac aacttcacct gaggcgagtt ttagcttatc gcgcagatcg tcggcttcgc
      241 ctttcttgat tccgataaat gtgaatttgc tcaatcgggg tcgttcaacg agttgaattt
      301 cgaggaagat gaggttatct tcgatgcgtt gtgcaattat tctgacatta tcgaacaatc
      361 cttgtctcca taatttttgt atcgccaagg taatttcttc tccgggcact tttatcctgt
      421 cgcctatctt caagccggta agcatagtta acacggatgc atcgagatac tgcacaccgg
      481 ttattgtaat tcctcctatc tcgtattctt tgggattgtc gtaatcgatg gatacttgag
      541 gagaacctat tgtaatttgt gcttgaacat aaaatgggac gaaatagata aacagggtaa
      601 aagcaatagg cagaatgaat ctgttggact tcatggagaa tatttttccg attaatggag
      661 taagaatttt actttggtca tgacgacaat tgttcgctta ttttgccgaa gcggcgttca
      721 cggcgttgat aatcggctat ggccaaaaaa aggtcttctt tacgaaaatc gggccaaaga
      781 acaggtgtaa aatagagttc ggtgtaagca atttgccaaa ggaggaaatt gctgatgcga
      841 tattcgccgc tggtacgaat taagagttcg gggtcgggaa ggaaaggcgc attcaagtat
      901 gccgatataa tttcctgagt aatttggctg ggaagtaaag tgccattttc tacttcacgg
      961 gcaattttct tcaccatttc agtaatttcc catcttgcac tatagctgat ggcgagtacc
     1021 aaatccattc gtgtaaaaga agaagtaatt tcgatggcat tttccaaagc ttgacgggtc
     1081 gatttttcca attggtcaag atctccaatg gctctgagtc ggatgttatt ttttgtgagg
     1141 gtatcggttt cattatgaat ggtttcaacc agtaaactca taattgcatc tatttcacat
     1201 tgaggccttt tccagttttc ggtggaaaaa gtataaagag ttagataacg tatgcccaat
     1261 tcggcggctg cttcagcggt ttctctaacg gcagtaatag catgctgatg ccccatcacg
     1321 cggtccatgc ctctttgcct cgcccagcgt ccattcccat ccataataat ggcaatgtgt
     1381 cgaggaagct ttcccatgtc gagtgaagct ttgatttcct gtaatctgga tgtttctgac
     1441 attccttaag gcatttttcc cttcatcaag ggttgaatat gaatttttgg caaatttaca
     1501 ataatcaatt tatcgaagaa ggcaataacg atctatacta ttatttagct tctgatttca
     1561 taaatcat
//
LOCUS       DAORPD010000472         1566 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_15485_length_1566_cov_3.121774, whole genome shotgun sequence.
ACCESSION   DAORPD010000472 DAORPD010000000
VERSION     DAORPD010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15485_length_1566_cov_3.121774"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1210)
                     /locus_tag="PK976_05980"
     CDS             complement(<1..1210)
                     /locus_tag="PK976_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase"
                     /protein_id="HRC67470.1"
                     /translation="MQKLVLLGDEAIGQAAIDAGLSAIYAYPGTPSTEINEYVMASKE
                     AKEGRIIASWTVNEKTAVESALGMSYSGKRSMACMKHVGLNVAADPFINSAITGVNGG
                     MIVVSADDPSMHSSQNEQDSRFYGNFAMLPILEPSNQQEAYDMIHYGFDLSEKFHVPV
                     LFRITTRLAHSRSGIERKAVRTQNPISLPENLKQFILLPAIARKRYQVLLSIQDALEE
                     ESENSGNNVYIDGSDKSLGIITTGLAYNYLMENFPDRTVPHPVLKITQYPLPRKMLQK
                     IVSECKEILVIEEGMPVVEETLKGILDHGIKIKGRLDGSLPRSGELNPNLVAVALGKP
                     MIKGDDVPSLIVGRPPSLCEGCPHTDSYFALNEAMAEYGKGRVFSDIGCYTLAALPPL
                     EAINSCVDMGA"
     gene            complement(1375..>1566)
                     /locus_tag="PK976_05985"
     CDS             complement(1375..>1566)
                     /locus_tag="PK976_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRC67471.1"
                     /translation="LERRLRINGINLSVDKVLNIAKTITNLKIKLPKSGETMTMIMLI
                     TKKHKSIAPLFDEKFWKNF"
BASE COUNT          437 a          351 c          292 g          486 t
ORIGIN      
        1 aagctcccat atctacgcat gaattaatgg cttcaagcgg gggcaatgcc gcaagcgtat
       61 aacagccaat atcggaaaat actctccctt tgccatattc agccatggct tcatttaggg
      121 cgaagtaaga atcggtatgt gggcatcctt cacaaagcga aggaggacga ccaacaataa
      181 gcgagggaac atcatccccc ttgatcatgg gtttgcccaa tgccacagcc actaagtttg
      241 ggttcaattc tccactgcgg ggtaaacttc cgtccaaacg accctttatc tttataccat
      301 gatctaaaat ccctttcaga gtctcttcca caacgggcat gccttcttcg attactaaaa
      361 tttccttgca ttccgaaact attttttgca acatttttcg tggtaaagga tattgagtga
      421 ttttcaagac gggatgtggg actgttctat cggggaaatt ctccatcaga taattgtatg
      481 ccaaacccgt agtaataatt cccaatgatt tatccgagcc atctatataa acattatttc
      541 cagaattttc cgattcttct tccagggcat cctgaatgct taatagaact tgataacgtt
      601 tgcgggcgat ggcaggcaag agtataaatt gtttcagatt ttcgggcaag ctaatgggat
      661 tttgagttct taccgctttt cgttcaattc ccgaacggct atgcgccagt cgagttgtga
      721 tgcgaaacag tacgggtaca tgaaacttct ccgacaaatc gaaaccataa tgtatcatgt
      781 cataggcttc ctgttggttg gaaggttcta aaatcggaag catagcaaaa tttccgtaaa
      841 aacgagaatc ctgttcattt tgtgaactat gcatggaggg gtcgtcagcc gaaaccacaa
      901 tcatgcctcc attcactccc gtaattgctg aattgataaa ggggtctgcg gctacgttca
      961 aacctacatg cttcatacaa gccatactac gtttccctga atacgacatg cccaaagccg
     1021 attccactgc agttttttcg ttgactgtcc agcttgctat aattcttcct tctttggctt
     1081 ccttcgatgc catcacatat tcgttgatct cagtggaagg cgttcctgga taagcataaa
     1141 ttgccgataa tccagcgtcg atggctgcct gtcctatggc ttcatctcct aataatacaa
     1201 gtttttgcat atcttaaaat gattgaaaaa ttatcatttc cctcggccta caaatttaag
     1261 gatttttaat ttgctggaaa gaaaatcaag agaaactacc tacccaatca atgaaagatg
     1321 aatgtcagac cctactcatt gaatcttgtc tcctgacttc gacaatgcgt caccctaaaa
     1381 atttttccaa aatttttcat cgaaaagagg tgcaattgat ttgtgttttt tagttatcaa
     1441 cattatcatg gtcattgttt caccactctt gggtagttta attttcagat tagtaattgt
     1501 tttggctatg ttcaaaactt tatccacact caaattaatg ccgtttatcc ttaatctcct
     1561 ttcaag
//
LOCUS       DAORPD010000473         1561 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_15578_length_1561_cov_2.157371, whole genome shotgun sequence.
ACCESSION   DAORPD010000473 DAORPD010000000
VERSION     DAORPD010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15578_length_1561_cov_2.157371"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1177)
                     /locus_tag="PK976_05990"
     CDS             complement(<1..1177)
                     /locus_tag="PK976_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67472.1"
                     /translation="MNKGTQAFATTSKMSLTGENNVEKALISTQHVDAPIPTVIPPIG
                     VHLYGNIYDGGVGGGEAQGFPLYAQIHIYNNNFDKTIFTDPITGAYDIANLMPGDYHV
                     DTTAINNGYLTKKEFLRFPSNGNYPREISLFVNSDVCTTPGYQPDVPYFYTFEFSNEG
                     FTSGGTTSFAVGEFTSGPGEGHSGKKGIATNPTGNYNPNENGYMLSPVINLSSFNDNE
                     IAIQWWEWRQIEEYNIWDQASLQVTKDGGTNWQTVWGPVGGGNGYGYQHQMIVLDSSY
                     GVPNFQMRYFFKSDNANQYEGWYIDDIAIYPLNSSIITTVFSNDFEPDDGNFYVSGTN
                     PSWALGQPTTGPGNAASGEYAWATNLSGDYNSSESSAITSPPIDLSRQTGLTPTLSFM
                     "
     gene            complement(1260..>1561)
                     /locus_tag="PK976_05995"
     CDS             complement(1260..>1561)
                     /locus_tag="PK976_05995"
                     /inference="COORDINATES: protein motif:HMM:NF024941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4127 family protein"
                     /protein_id="HRC67473.1"
                     /translation="LNYGRTKHHLDILVTRYLEDIAFGGYVREIVWYEMLPNLPEYSY
                     YDTKDHQGFMSELVKTEIIKFVENNMPTLKKHYEIEKLSLPWKRLYEIDLKVKYK"
BASE COUNT          441 a          325 c          306 g          489 t
ORIGIN      
        1 gcataaaaga aagggttggc gttaatccag tttgtcgtga aagatcaatc gggggggagg
       61 taatcgcgct actttccgaa gagttgtagt cgccactaag atttgttgcc catgcatatt
      121 cacctgaggc agcatttcca ggaccagtgg ttggttgacc caatgcccag ctagggttag
      181 tgccactcac ataaaagttt ccatcgtcag gttcgaaatc attcgaaaaa acggttgtaa
      241 ttattgaaga attcaatggg tagatagcaa tatcatcgat ataccaacct tcatattgat
      301 tggcattatc actcttaaag aaataccgca tctgaaaatt cggaacaccg taacttgaat
      361 ccagcacaat catttgatgc tgatagccat aaccgttgcc acccccaaca ggcccccata
      421 ctgtctgcca gtttgttcca ccatctttag ttacctgcaa actggcttga tcccaaatat
      481 tgtactcttc aatttgcctc cattcccacc attggatagc tatctcatta tcattgaaac
      541 tagagagatt tatcactggc gaaagcatat agccattttc attagggttg taattcccgg
      601 taggattagt ggctataccc ttcttaccgc tatgaccctc gccaggtcca ctggtaaatt
      661 ctcccactgc aaagctggtt gttccgccag aggtaaagcc ttcattagag aattcgaaag
      721 tatagaagta aggaacatct ggctgatatc caggagtagt acaaacgtca ctatttacaa
      781 atagactaat ttcacgaggg tagtttccgt tacttggaaa acgaaggaat tcctttttag
      841 tcaagtaacc attattaatt gcggttgtgt ctacatggta gtcaccaggc atcaaatttg
      901 ctatatcata agcacctgta atgggatcag taaaaatagt tttatcaaaa ttattattat
      961 agatatggat ctgggcatag agaggaaagc cttgagcctc tccaccacct actcctccat
     1021 catagatgtt gccatataaa tgaactccta tggggggtat aacggttggt attggagcat
     1081 caacatgctg cgttgaaatt aaggcctttt caacattgtt ttctccagtg agggacatct
     1141 tcgaggtagt tgcaaatgct tgagtgcctt tattcatgcc tgtaaggcta acaaataagc
     1201 ttgccaataa gataagtagt aatgtttttt taggttcaat ttttttcatt ttttcctccc
     1261 tatttgtatt tgacttttaa atcaatttcg tacagtcttt tccaaggcag acttaatttt
     1321 tcgatttcgt aatgtttttt cagtgtcggc atgttgtttt ccacaaactt gatgatttcc
     1381 gttttaacta gttcgctcat gaagccttga tggtctttgg tatcgtaata cgaatattcc
     1441 ggcaaattcg gaagcatttc gtaccagacg atttcgcgga cgtaaccgcc gaaagcaatg
     1501 tcttccaaat atcttgtgac caaaatatcc aaatgatgtt tggtacgtcc gtaattcaga
     1561 t
//
LOCUS       DAORPD010000474         1559 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_15603_length_1559_cov_2.583112, whole genome shotgun sequence.
ACCESSION   DAORPD010000474 DAORPD010000000
VERSION     DAORPD010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15603_length_1559_cov_2.583112"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(28..972)
                     /locus_tag="PK976_06000"
     CDS             complement(28..972)
                     /locus_tag="PK976_06000"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HRC67474.1"
                     /translation="MSESAFGLSDSIKSCRAVYFPEAKINLDGIEEESEWKQTNACGN
                     FIQQRPYEKTSPSQTTEFKVIYDDEFIYFFIQAYDSMPKKIERRLARRDDIEGDWAGV
                     QLDTYNDRTTAFTFFVNAAGVKFDGIESETDNMEDVDPTWDAVWEAKTVVNDKGWSIE
                     MKISFSQLRFDKNNTEGWGLQVTRYIHRNEEMSLWQYIPRNAPGWVRYFGRLNGLEHI
                     KRVPQIEAAPYVALQHETYKKEEGNPYRSGKNTLFRAGIDGKVSLASNVILDYTINPD
                     FGQVEADPSVVNLTAFETYFSEKRPFFIEGKNILDFQN"
     gene            complement(1159..>1559)
                     /locus_tag="PK976_06005"
     CDS             complement(1159..>1559)
                     /locus_tag="PK976_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HRC67475.1"
                     /translation="NLLLNGFNSYPHSCIKADALKYLDDMNSEYDLIILDPPAFAKHT
                     HQKSSAVRAYRHSNRQAIRKAKPGSFLFTFSCSQVIDNSLFSGIVHSAAIEAEREVKI
                     LQQMTQGPDHPVSIFHPEGQYLKGILLYVF"
BASE COUNT          467 a          319 c          287 g          486 t
ORIGIN      
        1 cagatgcaat tgagaagatt tggattttca attctgaaaa tccaaaatat tttttccctc
       61 gataaaaaaa ggccgttttt ctgaaaaata tgtttcgaag gccgtcagat tcaccacgga
      121 agggtctgcc tcaacctgtc caaaatcagg attgatggta taatcaagta ttacattcga
      181 agccaaactc actttcccat ctatgccagc tctgaaaagt gtgttcttac ccgacctgta
      241 tggattccct tcttcctttt tataagtctc gtgctgaagt gctacataag gagctgcctc
      301 tatctgtggt acacgtttaa tatgttctaa tccgtttaaa cgtccaaaat aacgtaccca
      361 tcccggagca tttcttggaa tatattgcca tagcgacatt tcttcatttc gatgaatata
      421 tctggttact tgcaaacccc aaccctcagt attattctta tcgaatcgaa gctgagaaaa
      481 cgaaatcttc atttcaattg accagccttt atcattcact accgtttttg cttcccaaac
      541 tgcatcccag gtaggatcga catcttccat attatcggtc tctgattcta tgccatcaaa
      601 ttttacgcct gcagcattga caaaaaaagt aaaagccgtt gtgcgatcat tataagtatc
      661 taattgtacg cctgcccaat ccccttctat atcgtccctg cgtgcaagtc gcctttcgat
      721 ttttttaggc atactgtcgt aagcctgaat aaaaaagtat ataaattcat catcataaat
      781 tactttgaat tcagtagttt ggctgggtga agtcttctca taaggacgct gctgaataaa
      841 attaccacac gcattcgtct gcttccattc gctttcttct tctattccat ccaaattaat
      901 ttttgcttca ggaaaataaa ctgccctgca gctttttatt gaatcgctca atccaaaagc
      961 tgactctgac atcgtaaaat aaaacaaaaa aattgcaggg attctaagaa ccgccgattt
     1021 attaaataac cctttaagta aatgcatgaa ttatttgctg gtgattgtaa tattcagacg
     1081 gcctttttta taaaatgtta caaaacgaaa aatccgttcc tgtttttagc taagctttaa
     1141 ccgtaaaggt gaagagattt aaaagacata aaggagaatt cccttaagat attgaccttc
     1201 gggatggaag atgctgacag ggtgatcggg gccctgtgtc atttgctgaa gaatttttac
     1261 ttcacgctcg gcttcgatgg ctgccgaatg tacaattcca gaaaaaagtg aattgtcaat
     1321 aacctgtgag catgaaaacg taaaaagaaa tgagcctggt tttgcctttc gaattgcctg
     1381 acggttacta tgacgatagg cgcgaacggc actggatttt tgatgagtgt gttttgcaaa
     1441 agccggagga tcgagaataa tcagatcata ttcggagttc atatcatcaa gatattttaa
     1501 ggcatcggct tttatacagg aatgggggta agaattgaag ccattgagaa gcagatttc
//
LOCUS       DAORPD010000475         1556 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_15658_length_1556_cov_2.087275, whole genome shotgun sequence.
ACCESSION   DAORPD010000475 DAORPD010000000
VERSION     DAORPD010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15658_length_1556_cov_2.087275"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            180..902
                     /locus_tag="PK976_06010"
     CDS             180..902
                     /locus_tag="PK976_06010"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HRC67476.1"
                     /translation="MIHISSIDSTNNYAARLLSENQMSDNTLIIADYQEQGRGHGSNR
                     WFSRPRKNLLASLYLIHTDILAVDQFVVNCIVSSAIAVFLENELKREVKIKWPNDIYV
                     NRQKIAGLLIESFITGERLTSSIIGIGLNVNERDFPPDIPNPTSMFLQDNQLRNPSQV
                     GISITNKILEMHTDKNQYSRARNIYLDRLYQKGVEANYLLLGKKIEAVITGIDNFGRL
                     ILRKGTYEYHCNFKEVVYLHNV"
     gene            complement(912..1307)
                     /locus_tag="PK976_06015"
     CDS             complement(912..1307)
                     /locus_tag="PK976_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67477.1"
                     /translation="MMNLKSNTIEVRHCPEDLFAYLTDLNRLQQALPPQVINYHVEGD
                     ACSFTIEGMTDISLLISMKIPYTRVVYSNAVDKPFSFSLVFDISPAAENKSMVSVAFE
                     ARANSFLSGILKKPMQNFVDLVSQKLNEL"
     gene            complement(1304..>1556)
                     /locus_tag="PK976_06020"
     CDS             complement(1304..>1556)
                     /locus_tag="PK976_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HRC67478.1"
                     /translation="TGGTSLKAVETLRKAGCTVKGMVAIFSYNFAVSNEAFKEAKIRL
                     LTLTDYDILIQQALENDYITQKDMESLIAWRQRPEKWGE"
BASE COUNT          478 a          308 c          303 g          467 t
ORIGIN      
        1 actgcttttt tttcatgcat tgcctcgatg atggcctgca aaattgcttg gtcgtcagct
       61 aaccttttcc gttttgccat ttacatccct ttgtatgaaa atagtttgca aaattactga
      121 ttttttaaag agcattattt ttgccagata tcaaagataa gaattttatg aatttgaaaa
      181 tgattcatat ttcttcaatt gattccacca ataattatgc cgctcgatta ttgtcggaga
      241 atcaaatgtc agataatact cttattatag ccgattatca ggaacaaggg cgaggacacg
      301 gttcgaatcg ctggtttagc agaccgcgca aaaatctgct agccagctta tatttaatac
      361 ataccgacat tctggctgtt gatcaatttg tggtaaattg catcgtctcg tcggcaattg
      421 ccgttttcct cgaaaatgaa ctcaaaaggg aagtcaagat taaatggcca aacgatattt
      481 acgtgaatcg acaaaaaata gcaggattat tgatagagag ttttattacg ggtgaacggc
      541 tcacatcgag cataattggg ataggcctca acgtgaacga aagggatttt ccccccgata
      601 ttcccaaccc tacctcgatg tttcttcagg ataatcaatt aagaaatccc agtcaggtag
      661 gtatttcgat tacaaataag attctcgaga tgcataccga taaaaatcaa tattccagag
      721 cacgtaatat atatcttgac aggctttacc agaaaggcgt cgaggcgaat tatttgctat
      781 taggaaagaa aattgaagcg gtcatcactg gcatcgataa ttttggccgc ctgatacttc
      841 gcaaaggaac atacgaatat cattgcaact tcaaagaagt ggtttactta cacaatgttt
      901 agctaaatga atcagagctc gtttaatttt tgagacacca aatcaacgaa attttgcatg
      961 ggctttttca gaatgccaga tagaaatgaa ttggccctgg cttcgaaagc taccgaaacc
     1021 atagatttat tttcagcagc aggtgaaata tcgaaaacca agctaaagga gaaaggttta
     1081 tcgacagcgt tgctgtaaac cactctggta taaggtattt tcatactaat aagcaaggaa
     1141 atatcagtca ttccttcgat ggtaaatgaa caagcatcac cttccacatg atagttgatg
     1201 acctgcggtg gaagtgcctg ttgtaaacga tttaaatcgg ttaaatatgc gaacaaatct
     1261 tcagggcaat gcctgacctc aatggtgttg cttttcaaat tcatcattct ccccactttt
     1321 caggccgctg ccgccaggca atgagcgatt ccatatcttt ttgggtaatg taatcgtttt
     1381 cgagagcttg ttgtataaga atatcgtaat cggtcagagt gagcagtctg attttggctt
     1441 ctttaaatgc ttcattcgac acagcaaaat tgtatgaaaa aatagccacc atacctttta
     1501 ctgtgcagcc agccttacga agagtttcta ctgccttcag actcgtccct ccagtc
//
LOCUS       DAORPD010000476         1548 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_15759_length_1548_cov_2.127261, whole genome shotgun sequence.
ACCESSION   DAORPD010000476 DAORPD010000000
VERSION     DAORPD010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15759_length_1548_cov_2.127261"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(314..>1548)
                     /gene="glgP"
                     /locus_tag="PK976_06025"
     CDS             complement(314..>1548)
                     /gene="glgP"
                     /locus_tag="PK976_06025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HRC67479.1"
                     /translation="WEKIYEVSDENIWAERMHQKEELIKFVKKHVNDDMTRRQENPKL
                     IFRVLESIKVEPLTIGFARRFAYYKRAYLLFFNMERLAKIVNNPRYPVQFIFAGKAHP
                     ADQQGQDLIKKIYTVSKQPEFLGKIIFLENYGMEMAKSLIRGVDVWLNTPMRLMEASG
                     TSGEKAVMNGVVNFSVQDGWWADGYKPGAGWAIKEKPTYTNDQFQDELDAETIYSTLE
                     EEIIPTYYDTDWNGVSPRWVAHVKNTIANIAPHFTMKRQLDDYYKKFYTKLFDRGELM
                     KQNDFELAKNISAWKRRVTRLWKDILVLNMKFPGSEHPALRQGETFHAEVTLDIGELS
                     PEDIGLELIFGRKINGTVDKLLFKNEMNQEIIDYHIVKFFCHIPITKAGVYDFSFRLY
                     PKHPLLPHRQDFCLVRYF"
BASE COUNT          425 a          312 c          295 g          516 t
ORIGIN      
        1 tatcgtggtt ttgatgattg cgttatgtat tatctaaaaa accattctta tcactctctt
       61 gactccccat catctgcaac ttctaaccct ttttttaaat ttcttttctt gacgttaaaa
      121 tctaactgtc ttggagactt gttaggtttg gacaaaaccc tataggtctt tggcgtctcg
      181 acaaaaaaat taccgaagtg cgctaaggat ttgaagggat tattgagggc atttctaaga
      241 tagtattttt gtaaaatata gataggcaat tgaagggtca ggagttctga ttttttcgat
      301 caaattccct tttttaaaaa tatctgacca gacaaaaatc ttgacgatga ggcagtaaag
      361 gatgttttgg gtacaaacgg aatgagaaat catacactcc ggctttagta ataggaatat
      421 ggcagaagaa ttttacgata tggtaatcga tgatttcttg attcatttcg tttttaaaca
      481 gaagtttatc cacagtgcca ttgattttgc gtccaaaaat caactcaaga ccaatatctt
      541 caggtgaaag ttcgccaata tcgagggtaa cttctgcatg aaaagtttca ccctgacgca
      601 gggcaggatg ttctgagcca gggaatttca tgtttaaaac caggatatct ttccacaggc
      661 gagttacgcg ccgtttccag gccgatatat ttttagccaa ttcgaaatca ttttgtttca
      721 tcagttcacc tctatcgaag agctttgtat aaaatttttt gtagtaatca tccaattgcc
      781 ttttcatggt gaaatgagga gcaatattcg caatggtatt tttgacatgg gctacccatc
      841 gtggtgaaac tccgttccag tcggtatcat aatacgtagg tatgatttct tcttcgaggg
      901 tagaataaat ggtttcggca tccagttcat cctgaaattg atcattggta taggtgggtt
      961 tctctttaat agcccaacct gcgcctggtt tgtagccatc ggcccaccag ccatcctgaa
     1021 cgctgaaatt gactactcca ttcatgactg ctttttcacc actagtaccc gaagcctcca
     1081 tcaatcgcat gggtgtattc aaccacacat ctactcctct gatcaaactt ttggccattt
     1141 ccattccgta gttttcgagg aaaatgattt tcccaagaaa ttcaggttgt ttgctcaccg
     1201 tataaatctt tttgatgagg tcttgaccct gttggtcagc gggatgtgct ttccctgcaa
     1261 agatgaattg tacgggataa cgtggattat tcacaatttt ggccaatctt tccatattga
     1321 agaacaacag gtaagccctt ttgtaataag caaatctacg ggcaaagcca atggtgagag
     1381 gttctacctt aatggattca agaaccctga aaatcaactt aggattttcc tgccggcggg
     1441 tcatgtcatc gtttacatgt ttcttaacga attttatcag ttcttctttc tgatgcattc
     1501 tttcagccca tatattctca tcacttactt cataaatttt ttcccagt
//
LOCUS       DAORPD010000477         1543 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_15840_length_1543_cov_3.639113, whole genome shotgun sequence.
ACCESSION   DAORPD010000477 DAORPD010000000
VERSION     DAORPD010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15840_length_1543_cov_3.639113"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..604)
                     /locus_tag="PK976_06030"
     CDS             complement(110..604)
                     /locus_tag="PK976_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67480.1"
                     /translation="MMKNTAKILALGMLFLFNSCEKDEDSTQVFALNSEAYKGFLFES
                     MKIINFPNSQNIIPDFTVLAQTNGSGEVLSPYLSNPNLENRFFLFDELEDLKNAQEVF
                     DSIQTTEINTFQKFALDIKPYQIWLIKTNSGSVGKILITETKTENIEDNPFAEIKFKA
                     KKLQ"
     gene            complement(757..1407)
                     /locus_tag="PK976_06035"
     CDS             complement(757..1407)
                     /locus_tag="PK976_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67481.1"
                     /translation="MNSKIPWIELILDFFIIATIAMYTNTLCHEWGHSLAGTFFGVKS
                     HPFDIHYTPFLFGIDENVNYSKVAQLPGWQGVTIAAAGPFVNFLFASLSLIFLLKFHW
                     QSTTGHRALLFFLYSLAFFGIGGWFNYTIIRGIVPRGDIANIVRFGSIPSWVIWVPGI
                     ITSAVLLVFFFGPTRLEFCKVFNLITQRAQIVQAIMVAFFFVMYQGSVIYNYLFKY"
BASE COUNT          495 a          276 c          275 g          497 t
ORIGIN      
        1 ttcgaatttg cttcatcagc cccagtaggc taaaccacgt gttagcaatt gggcatttat
       61 ttctttaagt atcattcggg ttgtcatatt aatatcttgg aagaataaac tattgcaact
      121 tctttgcttt aaatttaatt tctgcaaagg gattatcctc aatattttca gttttcgtct
      181 cagtaattag tatcttccca acagaacctg aatttgtttt aattaaccat atttgataag
      241 gcttaatatc taaggcaaat ttttggaatg tgttaatctc tgttgtctgt attgagtcaa
      301 aaacttcttg tgcatttttt aagtcctcta attcatcgaa gagaaaaaac ctattttcta
      361 aatttggatt cgataggtag ggagataata cttctccaga accgttggtt tgagctaata
      421 ctgtaaaatc tggaattata ttttgtgaat tagggaagtt gataattttc atgctttcaa
      481 agaggaatcc tttatatgct tcagaattta aagcaaatac ttgtgtggaa tcctcatctt
      541 tttcacaaga attaaaaaga aaaagcattc ctaatgctaa tattttagct gtatttttca
      601 tcatattgtg tcgtgtaatt gccttattgc caacgttttc agcatatacg aagtttcggc
      661 gaagccgaaa tttgagcgaa cgaagtgaga cgtatatgct gtgttatcgg aagttgcgat
      721 ttcgactttc tctgatacat ttcaaaaaat attacctcag tacttaaaca agtagttata
      781 gataacacta ccctgataca tcacaaaaaa gaatgctacc ataatcgctt gaacaatctg
      841 tgcccgttga gttattaggt tgaaaacttt gcaaaattcc aaccgtgtgg gtccaaagaa
      901 aaacacaaga agtactgctg atgtaatgat tccaggaacc cagataaccc aggaaggtat
      961 agatccaaac cgcacgatat ttgcgatgtc acccctcggt acaatcccac ggataattgt
     1021 gtaattaaac caaccgccaa ttccaaaaaa agcgagacta taaaggaaaa acaacaaggc
     1081 tctgtggcca gttgtacttt gccaatgaaa ttttagtaga aagataagag ataaagacgc
     1141 aaaaaggaag ttgacaaaag gtccagctgc tgcaattgta actccttgcc aacctgggag
     1201 ttgtgcaacc ttactgtaat ttacgttttc gtcgatgcca aagaggaatg gtgtgtagtg
     1261 tatatcaaaa ggatgagatt taacaccgaa aaaagttcca gccagactat gtccccattc
     1321 atggcataag gtgttggtat acattgcaat tgtggcaatt ataaaaaagt ccaatattag
     1381 ttcaatccat ggaattttac tattcatgta aggttgcctc tataatcata ttatcttttc
     1441 tccaaaagca atttcggata actcgtttat aggcgccact ccatatcgcc ttatttccat
     1501 ttttggatgt ataggcgcca taaagttgcg tttaccattc tat
//
LOCUS       DAORPD010000478         1541 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_15876_length_1541_cov_2.560565, whole genome shotgun sequence.
ACCESSION   DAORPD010000478 DAORPD010000000
VERSION     DAORPD010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15876_length_1541_cov_2.560565"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="PK976_06040"
     CDS             complement(<1..438)
                     /locus_tag="PK976_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67482.1"
                     /translation="MKTKSLTLSLVLVSLMVVTASAQSRKERKILYSSQYNYEIEPVG
                     VGQDGTKVFKVWGYGKDVNAAVMNAKEAAVAACIFKGLPGGAGSAPTPPICSQPNAEV
                     TYADYFDEFFKIGGKYLQYIAVTNDGDPAAEDRIKLKKGYKVAI"
     gene            complement(463..>1541)
                     /locus_tag="PK976_06045"
     CDS             complement(463..>1541)
                     /locus_tag="PK976_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67483.1"
                     /translation="EIQAALYDCWIYPEDSPEVKAEKLQKFEQLEIPIEFVTKTTHSL
                     SASQANPDTQLGKLTKQKSDDELLMELVQSGYDETLYYLERKYEDFMVKATIYKVKPI
                     QVKIGKKEGLKCDHRYFVYEYLYDEKTNTVKPVYRGVIRATSKIADNRQLATGEMPTS
                     TFYQTAGRKLQTGYLVRQQNDIGIEILAGYEMGEIGGPYGRLDFRLGRFIGLKAFFIY
                     LEGGGQQKEYEYHYPSYTKATTEDVTEDVTFIHYGVGLAQGLMLMRNMELRPYIGIGL
                     ESASSDEIDKADKGNLSTLFLKFGGNLAINLRHNIQLTGGINYYALIGNASNKDNSDL
                     GIKWDEIFQDRKGLSGLVGLKIMF"
BASE COUNT          404 a          327 c          311 g          499 t
ORIGIN      
        1 aatggcaacc ttgtatcctt ttttgagttt gatgcggtct tcggctgcag ggtctccgtc
       61 gttggttacg gcgatgtatt gaaggtattt acctcctatt ttgaagaatt catcgaaata
      121 atcggcataa gtaacttcgg cattgggctg tgaacatatt ggaggtgtgg gtgccgaacc
      181 ggctccaccc ggcagacctt tgaagataca ggcagcaact gcagcttctt tggcattcat
      241 tactgctgca ttcacatctt ttccataacc ccataccttg aaaacttttg tgccatcttg
      301 gccaacgcca acgggctcaa tttcataatt gtactggctg ctgtaaagta tcttacgctc
      361 cttgcgcgat tgagcactcg cagtaacaac catcaaactg accaatacta gtgatagggt
      421 aagtgatttt gttttcataa gcataatccg tttatattta ggttagaaca tgattttaag
      481 tcccactaag ccagaaagac ctttcctatc ctgaaatatt tcatcccatt ttattccaag
      541 gtcgctatta tctttattgc ttgcattacc gatgagtgcg taataattta tgccgccggt
      601 taattgtatg ttatgccgta agttgatggc aagattaccg ccaaatttta gaaacaacgt
      661 ggagagatta cccttgtcgg ccttatcgat ttcatcgctc gaagctgatt ctaatccaat
      721 gccaatgtaa ggtcgtaatt ccatattgcg cataagcatc aatccctgtg ccaaacctac
      781 accataatgg ataaaggtaa catcctcagt aacatcctca gtggtggcct ttgtatagct
      841 tggataatga tattcgtatt ccttttgttg gccaccacct tctaaataaa taaagaaggc
      901 tttaagacca atgaagcgac ccagtctaaa atcgagcctt ccataaggtc caccaatttc
      961 tcccatttcg tatccagcta aaatttctat tccaatatca ttttgttggc gaaccaaata
     1021 acccgtctga agttttcggc ctgcagtctg ataaaaggta gaagtaggca tttcgccagt
     1081 agcaagctga cgattgtcgg caattttcga agtagctctg attactccac ggtaaacggg
     1141 tttaacggta ttagtttttt catcataaag gtattcataa acaaaatagc ggtgatcgca
     1201 tttgagccct tctttcttcc cgattttgac ctgaatgggt tttaccttat aaatggtggc
     1261 tttaaccata aaatcttcat attttctttc gaggtaatat aaggtttcgt catagccact
     1321 ctgaacgagt tccattaaaa gttcatcatc cgatttttgt ttggtgagtt tgcccagttg
     1381 ggtatcaggg ttagcttgcg acgcactgag cgaatgagta gttttggtga cgaattcgat
     1441 ggggatttcg agctgttcga atttttgaag tttttcggct ttcacttctg gactatcttc
     1501 tggatagatc cagcaatcgt atagagcggc ttgaatttct t
//
LOCUS       DAORPD010000479         1540 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_15893_length_1540_cov_2.750842, whole genome shotgun sequence.
ACCESSION   DAORPD010000479 DAORPD010000000
VERSION     DAORPD010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15893_length_1540_cov_2.750842"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(237..1190)
                     /locus_tag="PK976_06050"
     CDS             complement(237..1190)
                     /locus_tag="PK976_06050"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005846514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HRC67484.1"
                     /translation="MHVCEILSKTNKTLFSFEILPPLKGGSFNDIFNSIEPLLEFKPA
                     YINVTYHQEEVVYKDRGNGLVEKMVVRKRPGTVGIAAALQYTCNIQVVPHIICGGFTR
                     EETENALIDLSFLGINNVLVVRGDPDRSQKTFIPEPDGHIHSLDLVKQIVDLNHGKYL
                     EEDLQNSNPTNFCIGVAGYPEKHPEAPNKASDLRYLKMKIDAGASYIVTQMFFDNRKY
                     FDFVDNCHEIGINVPIIPGLKPVAICQHLHLLPRTFGIDLPDDLAKELEKCKTSQEAR
                     NLGIEWTIHQTRELIAAHVPAVHFYTMGRSDNIYRIASACF"
     gene            complement(1200..>1540)
                     /locus_tag="PK976_06055"
     CDS             complement(1200..>1540)
                     /locus_tag="PK976_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HRC67485.1"
                     /translation="DENLSMEDILKEKYQGIRPAPGYPACPDHSGKVTLFEMLDAEKQ
                     IGISLTSHYAMYPAASVCGYYFSHLQAKYFNVGKINPDQIQDYALRKQLSIREVEKLL
                     RTHLNYEPLT"
BASE COUNT          450 a          309 c          295 g          486 t
ORIGIN      
        1 gtactctgcg gttaaaataa tgaacgtggg tttggagact taaaagttaa aaattaaaag
       61 gctgaggttg tatatgttag tattaaaaat acttcacaaa atcttattcg tactttcttc
      121 ctatttccca ttctctcaag tcccccttcg actccttttc catcaatcgc ccagcattat
      181 agtctcccat ctaaccttct tctaataaca tgcacaatga ttccttcgac atttttttag
      241 aagcaagcgc tggctattcg ataaatattg tcggatcggc ccatggtata gaaatgaaca
      301 gcaggaacat gagctgctat caactctcgg gtttgatgaa tggtccattc tattcctaaa
      361 ttccgcgctt cctgactggt tttgcacttt tccaattctt ttgccaagtc gtcgggaagg
      421 tcgatgccaa aggttcgggg caataaatgc agatgttgac aaatggctac gggtttcagc
      481 cctgggatga tgggaacatt tatacctatc tcatggcaat tgtcgacaaa gtcgaaatat
      541 ttccgattgt cgaagaacat ttgcgtaacg atataacttg ctccagcatc tattttcatc
      601 tttagatatc taagatcgga tgccttattg ggggcttcgg gatgtttctc gggataacct
      661 gccacaccaa tacaaaaatt ggttggatta gaattttgca aatcctcttc gagatatttt
      721 ccatgattta aatcaacgat ctgttttacc aagtcgaggg aatgaatatg accatcaggc
      781 tcgggaatga aggttttttg gctacggtcg ggatcccccc tcactacaag tacattatta
      841 ataccaagaa aactaaggtc gatgagtgca ttttcggttt cttcacgtgt aaaaccacca
      901 caaataatat gaggaactac ctgaatgtta caagtatatt gtaaggcagc agcaatacca
      961 actgtcccgg gccgctttct tactaccatc ttttctacca agccattgcc acgatcttta
     1021 tagaccactt cttcctgatg ataagtaaca tttatgtaag caggtttaaa ttccaataaa
     1081 ggttcaatac tattaaaaat atcattgaat gaacctcctt taaggggagg tagaatttca
     1141 aatgaaaaaa gtgttttatt ggttttcgag agaatttcac aaacatgcat gggtaaaaat
     1201 caagttaagg gttcataatt caaatgggtt cgcagcaatt tttccacttc acgtatgcta
     1261 agttgttttc gcaaggcata gtcttgtatc tgatcgggat ttatttttcc cacattaaaa
     1321 tattttgctt gaagatgcga aaaataataa ccacaaaccg aagctgctgg gtacatagca
     1381 taatgggaag taagacttat acctatttgc ttttcggcat cgagcatttc gaaaagagtg
     1441 acttttccgg aatggtcagg acaggcagga tagcccggcg ctggtctaat tccctgatat
     1501 ttttctttca ggatatcttc cattgaaaga ttttcatcct
//
LOCUS       DAORPD010000480         1533 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_16005_length_1533_cov_2.669824, whole genome shotgun sequence.
ACCESSION   DAORPD010000480 DAORPD010000000
VERSION     DAORPD010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16005_length_1533_cov_2.669824"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1533)
                     /locus_tag="PK976_06060"
     CDS             complement(<1..>1533)
                     /locus_tag="PK976_06060"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HRC67486.1"
                     /translation="YMHMSLAFGWFLLIVIGHLEVMVDRRSLNFPFYYAVFYRYYQPH
                     PDFAGARVFGALMDLLLLIILSGVALAILKRIKKQIFGMKRTTKLKPFDKIALTSLWL
                     IFPLRLLAESLSAAYYHNGSFMSNTLGAWFIQNVRVSAIYDLSWWLYSCSIGFFFIAL
                     PFSRYMHIPTEIVFIFLRHAGIMPKKYWNSYAQIQMYSCSRCGICLDACQLYEAGVSN
                     VQSVYFLQYIRNNENVDKSLFGCLICGRCQEACPVHINLNDLRTALRIKASKPLTFSY
                     GYTQMALQQKTDIIYFAGCMTHLTPPIKFSMARIFNFLGEKVWYMDEDKSICCGGPLM
                     LAGQYDSASQLIQKNTELIMASGAKTLILSCPICYKAFREDYVLPGISIKFHAVYLND
                     MIRQGRLPKINLGFKTIYHDPCALGRGSKIYDEPRYVIDSYSQRMRLKKEKAEALCCG
                     AGLGGINLPIETKNKVTFEAMTYFARFKPNVLITACPLCKKTFARDRRIPVIDIAELT
                     ARA"
BASE COUNT          483 a          307 c          318 g          425 t
ORIGIN      
        1 gctcgagctg tgagttcggc gatatctatt actggaatac ggcgatcgcg ggcgaaggtt
       61 tttttgcata aagggcaagc cgtgataagt acattgggtt taaatcttgc aaaataagtc
      121 attgcttcga aagtaacttt atttttagtc tctataggta ggtttatccc tcctaaacca
      181 gctccacaac ataatgcctc ggctttttct tttttaagac gcattctttg agaataagaa
      241 tcgatcacat aacgcggttc atcatagatt ttacttcctc ttcctaaggc acagggatca
      301 tgataaatgg ttttaaaacc taaattaatt tttggtaacc gtccctgacg tatcatgtca
      361 ttcagataga ctgcgtgaaa cttgatggag atacccggca aaacataatc ttctctgaag
      421 gccttgtaac aaatagggca tgaaagtatt agggttttgg ctccagaagc cataattaac
      481 tcggtgtttt tctgaatcaa ttgcgaagcc gaatcatatt gacccgccaa catcaagggg
      541 cctccacaac agatggactt gtcctcgtcc atataccaga ctttttctcc taaaaaatta
      601 aaaattcggg ccatggaaaa tttaatcgga ggcgtaaggt gagtcataca tccagcaaaa
      661 taaatgatat cggtcttttg ttgcaaggcc atttgagtat atccgtaaga aaaagtaagc
      721 ggtttgctgg ctttaatgcg aagagctgtt ctcaaatcat taaggttgat atgaactgga
      781 caggcttcct gacatcggcc acaaattagg cacccaaata gagatttgtc gacattttca
      841 ttgtttctga tatattgaag gaaataaacc gactgtacat tcgatactcc ggcttcgtat
      901 aactggcagg catccaaaca aataccacac ctcgaacagg aatacatctg gatctgtgca
      961 taactgttcc agtatttttt gggcataatt ccggcatgac gaaggaaaat gaatacgatt
     1021 tcggtaggga tatgcatgta gcgcgaaaat ggaagtgcta tgaaaaagaa tcctatcgag
     1081 catgaataaa gccaccaact gagatcgtaa atagccgaaa ctcttacatt ttgaataaac
     1141 catgcaccta aagtattgct catgaagctg ccattatgat aataggctgc actcaggctt
     1201 tcagccaata aacgaagagg aaatatgagc catagagaag ttagtgcaat cttgtcgaat
     1261 ggtttgagct tagttgtcct tttcatgccg aatatctgtt tcttgatacg cttgaggatg
     1321 gctaatgcaa ctcccgataa aattatgaga agtaataaat ccatcagagc gccgaagacc
     1381 ctagccccgg caaagtcggg atgtggttga taataccggt aaaataccgc ataataaaaa
     1441 gggaagttta aactacggcg gtcaaccatc acttcgagat gtccgatcac aatcagtaaa
     1501 aaccatccaa aagccaggct catgtgcatg tat
//
LOCUS       DAORPD010000481         1528 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_16095_length_1528_cov_2.663951, whole genome shotgun sequence.
ACCESSION   DAORPD010000481 DAORPD010000000
VERSION     DAORPD010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16095_length_1528_cov_2.663951"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /locus_tag="PK976_06065"
     CDS             complement(<1..896)
                     /locus_tag="PK976_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67487.1"
                     /translation="MKNNHFKYSTALFLGIITQLTQISWLRSIFALFDGNEILSGICL
                     AIWMLFTGLGTKINGQQFFGKHSIFPSIAFVMQGFFPLIMDVLAIAFRNHIFIAGVMP
                     PIHWVAAFIFVWLAPFCFLSGMLYAWLVLQADGPKIYGMESIGGMLAGIFSIVLLLLF
                     PNNQISFYLIIALAFINTTLWLNIQYRFKFLFTLVLFILIIVPLIKVNFPEKANQKLF
                     EGQHLLSTLETPIGRLHLTEYYGDTNIYVNGQPYCSSYRGPEPTENLHFTMLQKPYAK
                     SLLILGEIVPRLLEETQSWNFD"
BASE COUNT          531 a          261 c          268 g          468 t
ORIGIN      
        1 tggtcgaaat tccatgattg ggtttcctcg agcagccgtg gaacgatttc ccctaaaatc
       61 aacagagatt ttgcataagg tttctgtagc atggtaaaat ggagattttc agtcggttcg
      121 gggcctcgat aagaactaca atagggttgc ccattcacat agatattcgt atctccgtag
      181 tactcggtaa gatgaagcct gcctatggga gtctcaagcg tcgaaagcaa atgctggcct
      241 tcgaaaagtt tttgattggc tttttctgga aaatttacct ttattaaagg cacaataatg
      301 agtataaaca gaaccaatgt aaacaggaat ttgaaacggt attgaatatt tagccaaagg
      361 gtagtattga tgaaagcaag tgcaatgatc aggtagaaac ttatctgatt attcgggaat
      421 aaaagcagca agacaataga aaaaatacct gccagcatgc caccaatgga ttccatcccg
      481 taaattttcg gaccatcggc ttgcagcaca agccatgcat atagcattcc cgacaagaag
      541 caaaaaggag cgagccagac gaaaataaat gcagctaccc aatgaattgg aggcatgacg
      601 cctgcaataa aaatatgatt acgaaatgca attgccaaaa catccataat taggggaaag
      661 aaaccctgca taacgaaggc aatggatgga aatatactat gtttgccaaa aaattgttgc
      721 ccatttattt ttgttcccaa acccgtaaac aacatccaga ttgcaaggca aattccagaa
      781 agaatctcat tcccgtcaaa aagcgcaaaa atgctcctta accaacttat ctgcgtaagc
      841 tgagttatta ttccaagaaa taatgccgta gagtatttga aatgattgtt tttcattcgc
      901 ttagaaattt tttacaaaat aattccttta gcattaattc taacctataa attttccaac
      961 gaaaatacat ttttttattc cataataaaa atatgatggt aagccttttt atatatactt
     1021 actaatcaat atattttggg atatcctctt ctgaatttag tgttttcttg aagatgaaat
     1081 ctctgctaag gtaaaattta atcttcaagt aaattttcga aacagagtaa tataatttca
     1141 atgacgaata ttttctgata aaattctggt gaaaggcatt acaattcttt cgtaaaagtc
     1201 gttatcagtc aatggaatga caattaacga aggcaaataa attccacctc aagttttatg
     1261 aacttataat ttatttttcg gttaatttat agatattcgc gttttacagg catacctaaa
     1321 tcagccattt catccaatac tcgatgaatg agttgaactt tttcggaatg gttgaggaag
     1381 gctgatttga atccattgat tattaatacg ataagatctt tatcagtaaa tcaaaaattt
     1441 ttgatgacaa tcatatattc gtttgtaaga gtagtatttg aaattaaacg attatcggta
     1501 ttcagtgtaa ctctcaagcc ataatcat
//
LOCUS       DAORPD010000482         1526 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_16128_length_1526_cov_2.340585, whole genome shotgun sequence.
ACCESSION   DAORPD010000482 DAORPD010000000
VERSION     DAORPD010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16128_length_1526_cov_2.340585"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="PK976_06070"
     CDS             <1..232
                     /locus_tag="PK976_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HRC67488.1"
                     /translation="GEGCNICKHTGWIEILGCGMVDPAVLENCDIHSETYTGFAFGLG
                     IERITMGLYQIKDLRLFFENDTRFLHQFKAAF"
     gene            complement(266..769)
                     /locus_tag="PK976_06075"
     CDS             complement(266..769)
                     /locus_tag="PK976_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67489.1"
                     /translation="MKNSNHQLEAIGLRLHKRIFVYEAILLILFIGSLFPYYIGKLWA
                     GTLILAITLILAIIYFFRGFRPCENNKDLMLYQKFTGLAEAMAILGIASRILYMQSDL
                     LFLGISGFIIVSLLIIHFIDVKKQNQKIRLERQDLIRLSILLVLTVIFMVWWPPLPRL
                     FYGEMIL"
     gene            1054..>1526
                     /locus_tag="PK976_06080"
     CDS             1054..>1526
                     /locus_tag="PK976_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67490.1"
                     /translation="MLHQIFVVRFRAYIHYLLHAKSRYSVHSPFVFDLMENVFRDRHY
                     HEYYYPAETVRKLMLHSDTTIQMMDYGMGGKKAKDYQVKVSQIARYSAKGKKNGRLLA
                     RISSYFSPAVIVELGTSLGISALYLKAASPSSIVHTIEGSTEVAALARQNFQQAG"
BASE COUNT          488 a          300 c          275 g          463 t
ORIGIN      
        1 aggcgaagga tgcaatattt gtaaacatac cggatggatc gaaattctgg gctgtggcat
       61 ggttgatccg gcagttcttg aaaattgcga catccacagc gaaacttaca cgggatttgc
      121 ttttggcctt ggcatcgaac gcattaccat gggcctctat caaattaaag acctccgcct
      181 gttcttcgaa aatgatacgc gttttttaca ccaattcaaa gcggcttttt aggttacaag
      241 tcagcctcga aatctttctg cttttctata aaatcatttc accgtaaaaa agtcgaggca
      301 atggcggcca ccacaccata aaaattacgg tgagcaccaa taaaatactc aaccgtatca
      361 aatcctggcg ctcgagtcgt atcttctggt tttgcttttt tacatcaata aagtgaatta
      421 taagcaaact gactataatg aaccccgaaa tacctaaaaa aagtaaatca ctttgcatat
      481 atagaattct cgatgcgatt ccaagaattg ccatagcctc ggccaaaccc gtaaattttt
      541 gatataacat gaggtcttta ttattttcac agggtctgaa tcctctgaaa aaataaataa
      601 tggcaagaat aagcgtaatg gctaatatca aagttcctgc ccataatttc ccaatataat
      661 aagggaataa tgaacctatg aataaaatga gcagtatggc ttcgtaaaca aaaattcttt
      721 tgtgtaacct gagccctatc gcctcgagct gatgattcga atttttcata aacaaaattt
      781 tcaattggcg aaattactca tttgatttaa attttaccca aaacattcaa aatcacctat
      841 aataataaca tcccgttttt gccttgcttt ttatccaata attctaatcg cgaattaaat
      901 aaacctatct gtgtatgaaa aaattcgcca aatgagaaaa ttatcttttg cttttcatcg
      961 agtcataaat taaatgacat atctcgatgt aatattgaaa ttggacgatt gacctggaga
     1021 aagaaattaa taattcattc aagcttttta gttttgcttc atcaaatttt tgtcgtgaga
     1081 ttcagagcat acattcatta tttgcttcat gcaaagtcac gttattcggt gcattcgccc
     1141 tttgtcttcg atttaatgga gaatgtattc agagacagac attaccacga atattactat
     1201 ccagccgaaa cagttcgtaa actcatgttg cattccgata caaccattca gatgatggat
     1261 tatggaatgg ggggcaagaa agccaaggac tatcaggtaa aagttagcca aatagctcgt
     1321 tattctgcaa aagggaaaaa gaatggtcgt ttattggcac ggatatcctc atatttttct
     1381 ccggctgtga tcgtggaatt aggcacttcg ctgggaatta gtgctttata tctgaaggct
     1441 gcctctccat catccattgt tcataccata gaaggaagta ccgaagtggc ggcacttgca
     1501 cggcagaatt ttcaacaggc cgggtt
//
LOCUS       DAORPD010000483         1518 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_16272_length_1518_cov_2.464115, whole genome shotgun sequence.
ACCESSION   DAORPD010000483 DAORPD010000000
VERSION     DAORPD010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16272_length_1518_cov_2.464115"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..680
                     /locus_tag="PK976_06085"
     CDS             39..680
                     /locus_tag="PK976_06085"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67491.1"
                     /translation="MLGDVLLITDKHREAAASILNKVLEIRKSKMIIAISGESGSGKS
                     EISHVLAKALRTNGILTKVMHSDNYYRIHPLERKEWRIQHGIETVVGYNEYDWNNIYR
                     NIDEFRQGKVANMPCVDLVTEQVDQLITDFENIDALIIDGLYAIKTEGTDLNIFIELT
                     YHETKKAQMVRGKEPQNEYRARVLEQEHKMVQALKPLSHLLITPEYKVVPARG"
     gene            complement(792..1004)
                     /locus_tag="PK976_06090"
     CDS             complement(792..1004)
                     /locus_tag="PK976_06090"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HRC67492.1"
                     /translation="MKTLRFKTNLHCQHCIQKITPLLNAEEDIASWHVDLETSDKVLT
                     VKGDINPEKIISELEKIGYRAQLINE"
     gene            complement(1001..>1518)
                     /locus_tag="PK976_06095"
     CDS             complement(1001..>1518)
                     /locus_tag="PK976_06095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRC67493.1"
                     /translation="KQGIRSIMLTGDHKTSAKIIAEQVGIPTFYAELLPDDKRKIIDD
                     YMKKGETVLMIGDGINDAEAMASAQISATLGNSTSLALDLAGITLTHRKLLHLPAAIQ
                     LSRTTRRIIRQNLMWAFLYNVLCIPIAAGLLYPFTGELLNPMIAAAAMSVSSVLVVSN
                     SLRIKKIKLAI"
BASE COUNT          454 a          281 c          328 g          455 t
ORIGIN      
        1 acaagttcta tatttttgtt tcgtaaaatt ttgaaaatat gttgggagat gtattattga
       61 tcaccgataa acaccgtgaa gctgcagcaa gtatattgaa caaagtcctc gagattcgta
      121 agtccaagat gataattgcc attagtggag aatcgggaag tggaaaatcg gagatttcgc
      181 atgttctggc aaaagcactc aggacgaatg gcattcttac caaggtaatg catagcgaca
      241 actattatcg tattcatccc ttagaacgta aggaatggag aatacaacac ggaatagaaa
      301 ccgttgtggg atataacgaa tacgactgga acaatattta ccgaaatatc gatgaattcc
      361 gacaaggtaa ggttgctaat atgccatgcg ttgatttagt taccgaacag gtcgatcaat
      421 tgattacaga tttcgaaaat attgatgcgc tgattattga cgggctttat gcaataaaga
      481 cagaaggaac cgacttgaat atttttatcg aacttaccta tcacgaaacg aaaaaggccc
      541 aaatggtgag aggcaaagag cctcagaacg aatatcgtgc gcgtgtactg gaacaagagc
      601 ataaaatggt acaagcactg aaacccctat ctcatttatt aattactcct gagtataaag
      661 ttgttcccgc cagaggttaa agagttcatt ttggcctcga atctgcattg tcagtatttc
      721 caaaaccaat tctggtaaat actttaaccg tacattctat acgatctcga atcctttatt
      781 agaaacaaag gtcattcatt tatgagctgg gctctatatc ctatcttctc caattcactg
      841 atgatttttt cggggttgat atcacctttt acagtcaaga ccttatcgga ggtttcgaga
      901 tcgacatgcc atgaagctat atcttcttcg gcattcaaaa gaggtgtaat cttttgtatg
      961 caatgctgac aatgaagatt ggttttaaat cttagcgtct tcatattgca agttttattt
     1021 ttttgattct taaactattg gatacaacca gcactgagct caccgacatc gctgcagcgg
     1081 caatcatagg gttaaggagc tcaccggtga agggatatag caatcctgcg gctatgggga
     1141 tacataaaac gttatagagg aatgcccaca ttaaattctg gcggattatt ctccgcgttg
     1201 ttcgagagag ttgaatggca gcaggtaggt gaaggagttt tctatgggta agtgtaattc
     1261 ctgcaaggtc gagggcgagt gaggtactat tccctaacgt tgcactgatt tgggcactag
     1321 ccatggcttc tgcatcattg atgccatcac caatcatcag cacggtttca ccctttttca
     1381 tataatcgtc gataattttt cgtttatcat cgggtagaag ttcagcatag aaggttggta
     1441 taccgacttg ctcggcaatg attttagctg aagttttatg gtcgccggta agcataatag
     1501 atcgtattcc ttgttttt
//
LOCUS       DAORPD010000484         1518 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_16276_length_1518_cov_2.132604, whole genome shotgun sequence.
ACCESSION   DAORPD010000484 DAORPD010000000
VERSION     DAORPD010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16276_length_1518_cov_2.132604"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..1265
                     /locus_tag="PK976_06100"
     CDS             18..1265
                     /locus_tag="PK976_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRC67494.1"
                     /translation="MRDIFEKIATNKGPLGQYGEDAFGYYMFPKLEGEIGHRMIFGGR
                     ERIIWSLNNYLALANHPEVRKVDAEAAARWGLAYPMGARMMSGQTIYHEQLERELAEF
                     VRKEDAFVLNYGYQGMISVIDSLVDRKDVIVYDSDAHACIIDGVRLHLGKRFVFPHNH
                     MENLEKHLQRAAHLAEENRGGILVITEGVYGMSGDLGKLCEIIELKKKYPFRLLVDDA
                     HGFGTMGETGAGTGQYLGVQEGIDIYFGTFAKAMAGIGGFVAGSKLVTDYLRFNMRSQ
                     TYAKSLPLAMVIGALKRLEIIRTHPELREKLWLIANELQKGLLNAGFNIGNTQSPVTP
                     VILSGSVSEATGLIYDMRESFDIFCSIVTYPVIPRGQILVRLIPTADHTLEDVSYTIE
                     AFSKLKDKLERGEYGHDNTVRSF"
BASE COUNT          429 a          256 c          392 g          441 t
ORIGIN      
        1 ttaagagata aacttatatg agagatatat tcgagaaaat agcaacaaac aaggggccac
       61 tcggacaata tggagaggat gcttttggat attacatgtt tccgaaactc gaaggggaga
      121 ttggtcatcg gatgattttt ggtggcaggg aaagaatcat ctggagtttg aataattatc
      181 ttgccttggc caatcatcct gaggttagga aggtggatgc cgaggctgct gccagatggg
      241 gtctggctta tcctatggga gcccggatga tgtcgggaca aacgatttat cacgaacaac
      301 tggaaaggga attagccgaa tttgttcgaa aagaagacgc ttttgtgctc aattatggtt
      361 atcagggaat gatttcggtg attgattcat tggttgaccg taaggatgta atcgtttacg
      421 atagcgatgc tcatgcctgt attatagatg gtgtaagact acatcttggc aaacgttttg
      481 tttttccaca taatcacatg gaaaatctcg aaaaacatct tcagcgggcg gctcatcttg
      541 ccgaagaaaa cagaggagga attttggtga ttaccgaagg agtgtacggg atgtcgggcg
      601 atttgggaaa actctgcgaa attatagagt taaaaaagaa ataccctttc agacttttgg
      661 tggatgacgc acatggtttt ggaacgatgg gagaaacagg ggctggtacg gggcagtatc
      721 tgggagttca ggagggcata gatatctatt ttggcacctt tgccaaggcc atggctggaa
      781 ttgggggatt tgtggcaggc tcgaaattgg ttaccgatta tctccgtttt aatatgcgtt
      841 cgcaaaccta tgcaaaatca ttgcctctgg ccatggtaat aggagcattg aaacgactcg
      901 aaattatccg tactcatccc gaattaagag aaaaactctg gcttattgca aatgaacttc
      961 aaaaaggttt attgaatgca ggtttcaata tcgggaatac tcaatcaccg gttactccgg
     1021 ttattttgtc gggaagcgta tccgaggcta ccggacttat ttacgatatg cgcgaatcgt
     1081 tcgatatttt ctgtagcatc gtaacttatc cggtgattcc ccgagggcag attctcgtcc
     1141 gccttattcc caccgctgac catacgctcg aagatgtatc ctataccata gaggcatttt
     1201 cgaaacttaa ggataagctc gagagaggtg aatatggcca cgataatacc gtgagaagct
     1261 tttagaaccc ctttttgtat ctatccgagt caaaaacatg gcttggattt ttttaaattt
     1321 atcatggatt ttgtgtagca aggtgaatga ccgttgcaga tagtttatag gtaacctcca
     1381 ctcaggagga tggattgaag ggggcaattc gatatttatt ttggtctatg tagatttttt
     1441 ggagaaggga taaaacttga atggttggta ttttattttg ggagaagata aaaaagttga
     1501 tgggtgtgga gctctgct
//
LOCUS       DAORPD010000485         1517 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_16295_length_1517_cov_1.848837, whole genome shotgun sequence.
ACCESSION   DAORPD010000485 DAORPD010000000
VERSION     DAORPD010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16295_length_1517_cov_1.848837"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1233)
                     /locus_tag="PK976_06105"
     CDS             complement(<1..1233)
                     /locus_tag="PK976_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008151057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRC67495.1"
                     /translation="MALSENFDEKSELKKKFHQRKGFYILLGFLVGIVVIASLYQTSV
                     YFSTNESCMICHVHPHATDSWKLSTHVNNASGVMTNCVDCHLPPIENTWDYYTSKAAL
                     GLRDVWGYLTKDTADFDWDQKSQLEYAVKYIPNESCVKCHQNLFPEGVTDDAVTAHLY
                     YEENAEKLDLQCISCHLDAGHYNPNYIHGKMTGIPIAANQVVDTGKFYKEPAIVTRFE
                     NFIEQIPGTAVSFKMIAIKGGKFNMGSPEKEPFHKPDESPVHEVTLSPFFMAEVETTW
                     DEYWAFYTQTMSEGRTPPEVVYENNLKALDVDAISGPTPPFGFPDQGWGSGDRPAITM
                     THYAAEVFCQWLSKKTGKKYRLPTEAEWEYAARGGTQMPYFFPGNPKDFSDRGFWRKF
                     FKAKTDPIASYVIYNKNSN"
BASE COUNT          403 a          322 c          319 g          473 t
ORIGIN      
        1 gttgctgttt ttattataaa ttacatacga tgcgatggga tctgttttgg ctttgaaaaa
       61 tttgcgccag aatcctcgat ccgaaaaatc tttaggattg cccgggaaga aataaggcat
      121 ttgagttcct cctcgtgcag catactccca ttcggcttcg gttggaaggc gatatttttt
      181 ccctgttttt ttcgatagcc actggcaaaa tacttcagct gcatagtgcg tcatcgtgat
      241 ggccggtctg tcacctgatc cccatccttg gtcaggaaat ccgaaaggtg gcgttggccc
      301 cgaaatagca tctacatcca atgctttcag attattttca taaaccactt ccggaggtgt
      361 gcgtccttcg ctcatcgttt gggtatagaa agcccaatat tcgtcccatg ttgtttctac
      421 ttcggccata aagaaagggc tgagggttac ttcgtgaaca ggcgactcgt ccggtttatg
      481 aaacggctct ttttcgggac ttcccatatt gaattttccg cctttaatgg caatcatctt
      541 aaaagaaacg gctgttccgg gtatctgttc gatgaaattt tcgaatctgg ttacaatggc
      601 aggctcttta tagaacttac cggtatccac aacctgattt gccgcaatgg gaattcccgt
      661 catcttaccg tgaatgtaat ttggattata gtggccggca tctaagtggc aactaatgca
      721 ttgcaaatcg agcttttctg cgttctcttc ataatacaga tgtgctgtaa ccgcatcgtc
      781 ggttactcct tcaggaaaca gattctgatg acatttcaca cacgattcgt tggggatgta
      841 tttaacggcg tattccaatt gcgatttttg atcccaatca aagtcggcag tgtccttagt
      901 caggtatccc caaacgtcgc gcaatccgag ggcagctttc gacgtgtaat aatcccaggt
      961 attttcgatg ggaggaagat gacagtcaac acaatttgtc atcacaccac tcgcattgtt
     1021 gacatgtgtg gagagtttcc atgaatctgt agcatgcgga tgaacatggc atatcatgca
     1081 cgattcgttt gtagaaaaat aaaccgatgt ctgataaagc gacgcaataa ccacgatacc
     1141 aactaaaaaa ccaaggagaa tataaaaacc ctttcgttga tgaaatttct ttttgagttc
     1201 actcttttcg tcgaaatttt ccgataatgc cattctcttg atgttgaaga tgtgaaggtc
     1261 aaaacttgtt tactttttta aactggctgt aaaaatagcc atttttatta tgattccgag
     1321 aaattatgag ataaaaatgc aattatctcg atgtattctt gcaacagaat tacatttgag
     1381 gcaattccca aggggctcga taggagggaa ttaagtagct gttagcttct tcatcatgga
     1441 taaattttgt attgttagcg tcccaatata gccttcttcc acacttcagt gctatgttcc
     1501 ccaagtggca tactcga
//
LOCUS       DAORPD010000486         1515 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_16324_length_1515_cov_2.530137, whole genome shotgun sequence.
ACCESSION   DAORPD010000486 DAORPD010000000
VERSION     DAORPD010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16324_length_1515_cov_2.530137"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            438..>1515
                     /locus_tag="PK976_06110"
     CDS             438..>1515
                     /locus_tag="PK976_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease Q family protein"
                     /protein_id="HRC67496.1"
                     /translation="MSFIADLHIHSHFSLATSKELTPEYLHLWARRKGIDVIGTGDCI
                     HPNWLSELQEKLEPAETGLYRLKTIYRLAEENIPESQEPLFLLTTEISNIYKKGGKVR
                     KVHNVLMIPDFRTASLIQQKLKLLSFNITSDGRPILGMDSYELFRLVLDINPQCVLVP
                     AHIWTPWFSVLGASSGFDSIEECFEDLTPHIHTLETGLSSDVPMNRVVGKLDRFHLIS
                     NSDAHSPDRLGRNANIFFCDKSFDAIIEAMASHRSETIDLFPQEGKYHFAGHRKCNVS
                     LPPDEAAYHQGICPVCYKKLTSGVLDRVFQLASKTLPDEESISPRFHYIIPLPEIIAE
                     IIHTKSASKKVMEKYFQILKILGPE"
BASE COUNT          452 a          337 c          280 g          446 t
ORIGIN      
        1 acttcgaaat atcagttcgg gaagagccaa ttattccatg gaattctttc aatacatgcc
       61 actgccgcaa actttacagg aagaagtgat taaaaaagcc atcgaaaaga acaaaaaata
      121 aacctccact gcttggctaa atcgttgttg gactaaattt cctcctacaa agcaaaatgt
      181 tcttctaacg aaacagccgg gatccatatt tagacataaa tctgcaattt ataggtatgg
      241 cttcgataat caccaaaggc agaatccaag ttgaagctgt attttttgaa gattctcatt
      301 caatggtttt tctgaacatt cgctcccaat caaataaccc atcctttcca atttgatgtt
      361 ggcaagttga gaattcaaga attcctctcc cttttgtttt ctcttaactt cgcaacaata
      421 attttaatac cattccgatg tcatttattg ctgacttgca tattcattct catttcagtc
      481 ttgccaccag taaggaactt acgccggagt atcttcatct ttgggctagg cgaaagggca
      541 tagatgtaat agggacaggc gattgcatac atcctaactg gctttcggaa ctccaggaaa
      601 aactcgaacc tgccgaaacg ggattatatc gattaaaaac aatttaccgc ttggccgaag
      661 aaaacatccc cgagtctcag gaacctctat ttcttctgac taccgaaatc agcaacatct
      721 ataaaaaggg aggcaaggta agaaaagtac acaatgtact gatgattccc gatttccgta
      781 ccgcaagcct catacagcaa aaactaaaac tcttgagctt taacattact tcggatggaa
      841 gacccatttt aggaatggat tcttatgaat tgtttcgttt agtgttggat attaatccac
      901 agtgtgtatt ggtacctgct catatctgga cgccatggtt ttcggttctt ggtgccagtt
      961 caggcttcga cagtatagaa gaatgtttcg aagatctaac cccacatatc cataccctcg
     1021 aaactggcct ttcgagtgat gtacccatga accgtgtggt aggtaagtta gatcggtttc
     1081 acctgatttc caattccgac gcccattctc ccgaccggct tggccgaaat gctaacatct
     1141 tcttctgcga taaatccttc gacgctatta ttgaggctat ggccagtcat cgatccgaaa
     1201 ccatcgatct tttcccgcag gaagggaaat atcattttgc aggtcataga aaatgcaatg
     1261 tttcattacc acccgatgaa gcagcttatc atcagggaat ttgtccggtt tgctacaaaa
     1321 aactcacttc aggcgtgctc gatagggtgt ttcaattagc ttcgaaaact ctccccgatg
     1381 aagaatctat ttcgccccgt tttcattata ttattcctct gcctgaaatt attgcagaaa
     1441 ttattcacac aaaatctgcg tcgaagaagg taatggagaa atattttcaa attttaaaaa
     1501 tcttaggtcc ggagc
//
LOCUS       DAORPD010000487         1513 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_16358_length_1513_cov_2.469136, whole genome shotgun sequence.
ACCESSION   DAORPD010000487 DAORPD010000000
VERSION     DAORPD010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16358_length_1513_cov_2.469136"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..775)
                     /locus_tag="PK976_06115"
     CDS             complement(<1..775)
                     /locus_tag="PK976_06115"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRC67497.1"
                     /translation="MHKFTRIFYLIFIYTVLSGIQSYSQNSLSENLSLGVMTFNIRLD
                     TREDGKNAWPYRKAAVIEMLQNTHPAIIGFQEVLHHQVEEIAKGLRGYVHFGVGRDDG
                     KQGGEYCPIFFDSKVFSLIDSKTLWLSETPDLPGSLGWDAGCKRLVSVVLLKEKSSGK
                     ELYVLNTHFDHQGEKARIESAKMISRLCQEMIQNAPVILLGDLNASSETEIYRILTEQ
                     SSLKDAQTIAERKDEVDNCTFTGFDGDSCEHIDFIWMPQN"
     gene            complement(787..1080)
                     /locus_tag="PK976_06120"
     CDS             complement(787..1080)
                     /locus_tag="PK976_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67498.1"
                     /translation="MDLIIFISVYLISCFVIAFMAYKKKINWWLALIISVIVTPVGGF
                     IYYQSNNPIKAYKEVRYKCQRCGFYFTEKATFCPFCEHDGHKIPLKKIYVDMT"
BASE COUNT          465 a          299 c          259 g          490 t
ORIGIN      
        1 cattctgggg catccatata aagtcgatat gctcgcatga atctccatcg aaacccgtaa
       61 atgtgcaatt gtcgacttca tctttccgtt ctgctatggt ttgggcatct ttcagtgaac
      121 tttgctctgt taaaattcta tagatttctg tttctgacga ggcattgaga tctcccagaa
      181 gaataaccgg tgcattttga atcatctcct ggcagagcct gcttatcatt ttggcgcttt
      241 ctattcgagc tttttctccc tgatgatcga aatgagtatt gagcacatac aattcttttc
      301 cacttgattt ttccttcaat aaaacgacgc tgactaatcg tttacagcct gcatcccatc
      361 ctaatgaacc gggtaaatca ggtgtttcac tcaaccataa ggtcttacta tctattaatg
      421 aaaaaacttt agaatcgaaa aaaatagggc aatactcgcc tccttgcttt ccatcgtcgc
      481 gtccaacgcc aaaatggaca taaccacgta aaccctttgc aatttcttca acctgatgat
      541 gtaagacctc ctgaaatcct ataatagctg gatgtgtgtt ttgcaacatc tcgataacag
      601 cagccttgcg atatggccat gcattcttac catcttctcg agtatccagc cttatgttga
      661 atgtcatcac tcccaatgaa aggttttcgg atagggaatt ttgcgaatat gactgaattc
      721 cacttaaaac tgtataaata aaaattagat agaaaattct tgtgaactta tgcatgaaaa
      781 aaagttttag gtcatatcaa catatatctt tttcaagggt attttgtgcc catcatgttc
      841 acaaaaaggg caaaaggtgg ctttttcggt aaagtaaaaa ccacatcgct ggcacttgta
      901 acgaacttct ttatatgcct tgattggatt gttgctttga taataaataa aacctcctac
      961 tggcgttact atcacactga taattaaagc aagccaccag ttgatctttt tcttataagc
     1021 catgaaggca atgacaaagc atgaaatcaa ataaacactt ataaatatga ttaaatccat
     1081 tgggaatgaa attcgataga ttagaaatcc gaatgcaaag ctatgaatgt ttttaattca
     1141 tcaatacccc ataattaatg gttggttcct tcgaccaaaa gacttgtgtt attagctcat
     1201 ctatacgctg accgtcaatt ttattcataa ttaacaatga ataaatttga aaatatcgtt
     1261 gtttagaatg agtcaaaaat atagattctt cataaaactg acagatgtaa acgatcctta
     1321 tggcgagagg atgaaaatgt tgaattattt tcgggaaacg atcttttcca tgatatgatg
     1381 ttcgggtgat ggacatggtc gatcatggtt taaatatatc gatcgggaag caaattcttt
     1441 acatatcgat cttttccact ttaccattca accggctttt ttcggccctg aaacccatga
     1501 tatggctttc gat
//
LOCUS       DAORPD010000488         1513 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_16361_length_1513_cov_2.252401, whole genome shotgun sequence.
ACCESSION   DAORPD010000488 DAORPD010000000
VERSION     DAORPD010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16361_length_1513_cov_2.252401"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="PK976_06125"
     CDS             complement(<1..532)
                     /locus_tag="PK976_06125"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRC67499.1"
                     /translation="MKTNERNILLILLGSSFLIRFLLSFWLELGNDEVYYYTYALYPA
                     WSYFDHPPMVGWVIRIFSLNLLVQGEWAVRFASVALGTLNTYLIYVLGKFIKNREAGI
                     YAALLYTSSIYCFVIFGLFILPDTPQSTFWLLAIFAFLKSIISSELNKNSRSWLLAAG
                     VFAGLAMLSKYTSAFLW"
     gene            complement(519..>1513)
                     /locus_tag="PK976_06130"
     CDS             complement(519..>1513)
                     /locus_tag="PK976_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67500.1"
                     /translation="GRGNSNEKIEKIYSSLGYDIIATSDYQRINRFGIRKPSYIPTYE
                     HGYGIHKTHQICLGSQKVLWRDYPFYQGLNHKQHILNCLGIQNEIIAIAHPDLRDGYL
                     KEEMKYLSGYHLMEVFNRARTSLAHWDSALSSGYPAFLLANDDAHNVTNPYEIGRYAT
                     FVQSDTLVARHILEALKKGRAYGVRIAYKEGETLEEKKLHALRLPSLKEVSFSNDTLK
                     IEVSPSASEFRFVGQNGVILKNVKETNFAYYVIKPSDTYIRTEISFPSQDIIFLNPIF
                     RYQGGKITWPIRYIINQQASFIHYILWGSGGLILLFMSGLLIKKHHFRKAHENQ"
BASE COUNT          475 a          279 c          310 g          449 t
ORIGIN      
        1 cccataagaa ggccgaggta tatttggaaa gcatggccaa acccgcgaag acgcctgctg
       61 caagtagcca tgaacgggag tttttgttca gttcggatga gataatactc ttcagaaatg
      121 caaatattgc cagcaaccag aaagtgcttt gaggagtgtc gggcagaata aataaaccga
      181 aaataacaaa acaataaata gaagatgtat aaagaagggc agcataaatg cctgcctctc
      241 tgttttttat aaatttaccg agaacgtaaa ttagataagt attgagtgtt ccaagagcta
      301 ccgaggcaaa tcgtactgcc cattcgcctt gaactaaaag attgagcgaa aaaattcgga
      361 taacccatcc aaccattggc ggatggtcga aatatgacca ggcaggataa agtgcatagg
      421 tgtaataata aacttcgtca ttgccaagct ctaaccaaaa cgacagtaaa aatcttatca
      481 gaaatgagct ccctaacaaa atgagaagta tatttcgttc attggttttc atgcgctttc
      541 ctgaaatggt gttttttgat taataaacct gacataaaaa gaagaattaa ccctccgctt
      601 ccccagagta tataatgtat aaaactggct tgctgattaa taatatatcg aatgggccat
      661 gtgatcttgc ctccctgata acgaaaaatg ggattcaaaa agattatatc ctgagaaggg
      721 aagcttattt cggttcgaat ataggtatcg gagggtttga ttacataata ggcgaaattg
      781 gtttctttta catttttgag aattactcca ttttgaccga caaacctgaa ctccgaagct
      841 gatggtgata cttctatttt tagggtatca ttcgaaaagc ttacttcttt tagtgaaggc
      901 aatcgaagag catggagttt tttttcttct aaagtctccc cttctttata ggcaattctt
      961 acgccataag ctctgccttt tttcaaggct tccaatatat gtcgagcaac gagtgtatct
     1021 gattgtacaa aggtggcata tcttccaatt tcgtaggggt tggttacatt gtgagcatcg
     1081 tcgttggcaa gaaggaaagc aggataaccc gaactaaggg cactatccca atgagcaaga
     1141 ctggttcgcg ctctgttgaa tacctccatc aggtgatacc ctgataaata tttcatttct
     1201 tcttttaggt acccatcacg taaatcagga tgggcaatgg cgatgatttc attttggatg
     1261 ccgaggcagt ttaggatatg ttgtttatgg ttcaaacctt gataaaacgg gtaatcacgc
     1321 caaagaacct tctggctacc taaacagatt tgatgtgttt tatgaattcc ataaccatgt
     1381 tcataggttg gaatatagga tggttttcga attccaaaac gattaattct ttgataatcg
     1441 gaggtagcta taatgtcata acctaaactg gaataaattt tttcaatctt ctcattggaa
     1501 ttgcctctcc ctg
//
LOCUS       DAORPD010000489         1513 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_16365_length_1513_cov_1.744856, whole genome shotgun sequence.
ACCESSION   DAORPD010000489 DAORPD010000000
VERSION     DAORPD010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16365_length_1513_cov_1.744856"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="PK976_06135"
     CDS             <1..544
                     /locus_tag="PK976_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67501.1"
                     /translation="QQYEGILKMIFLWVYVYGLVAFFGALAVGTIINKGFGFVVIYIY
                     LMDTAKLLISLCSLGALLFIGVLSRSLFINSANVYFEEINFRNVKYVIRYHIVYPALL
                     GSLVIILFKLPKITPYELFTLSTIIIFLLPVWTSPGIMGERIFEDSGNLTLRRKMLIF
                     SFSILVLFRLFTDPRLLASR"
BASE COUNT          419 a          245 c          309 g          540 t
ORIGIN      
        1 tcaacagtat gaaggtatcc ttaagatgat attcttatgg gtttatgttt atggtctggt
       61 tgctttcttt ggagctctgg ccgtgggtac catcatcaac aagggttttg gttttgtggt
      121 tatttatatt tatttgatgg atacagccaa actgcttatc tcgctttgtt ctctgggtgc
      181 tttgctcttc ataggggttc tgtcgagatc tttgttcata aattctgcca atgtgtattt
      241 cgaagaaatc aacttccgta atgtcaagta tgtcatcaga tatcatatag tttaccctgc
      301 cttattgggt agccttgtta tcatcctgtt caaacttccc aaaattactc cttacgaact
      361 ttttaccctg agcaccatca ttatttttct acttcctgta tggacatcgc caggaattat
      421 gggggagaga attttcgaag acagtggaaa tttgactctg agacgtaaaa tgttgatttt
      481 ttctttctcg atattggttc ttttccggct gtttaccgat cctcggttat tagcgtcaag
      541 atagtgtgga ttggcctacc ctgttttcga ttcagattga aggaaatttg ttttcagcac
      601 attttcgaca aaatccttta gttcgcccgg agtgaatggt ttagccagaa aatggaagaa
      661 accctctctc agcaatcttt ctggtttcga tttgaatgcg ttagcagcca taacaacgat
      721 agggcctcga attctatcgg aatatttata tcggaatgat gagaaaaacg ttcgaaacag
      781 atgttggcaa agttgcggtt gtttttctgt atcaacactc aggttttttc gatgtgggtt
      841 tttataaaat agagtagggt gattgaggga gtaaaatttt cggaaatgat cctgaagaaa
      901 aatttccctg cctatggaag gctgtttaat cctaaaaatt tttactcgga taataaaatg
      961 ccatagtagc tcatgactgg gctcatttcg attggaatct atttttattc acttcgatat
     1021 cccattaacg gtttcgagat tgtttacctc atgatgccga acaaacccta tgaaaatttt
     1081 caaacttaat ccgaaaatcc ttggcttatt aaaatcggat aaatcgtcga atttatttac
     1141 atgtatgggg tcggtatttc atgagtgaac ttctgatctt agttcaatat ttagaagggg
     1201 aaagggaaat aaaagtctct tccatacgtt tgttttgttg taaaatatgg atctgttggc
     1261 ttcatcatga atcgggaagc agaatattta tcgcattcag gcattttgta cgattgtaaa
     1321 taaatagaat aaataattta gttgagattt taaaggtttt atccggaatt gtgaagagaa
     1381 tcgataaaat atttaggaat agctttatca gccgtcactt tatagaaaat tttcgatgga
     1441 aatatatcat gcttaataat agtatggaaa gatgggggtt attgtccatt ttccggttag
     1501 ttgccatttc gag
//
LOCUS       DAORPD010000490         1512 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_16374_length_1512_cov_2.470830, whole genome shotgun sequence.
ACCESSION   DAORPD010000490 DAORPD010000000
VERSION     DAORPD010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16374_length_1512_cov_2.470830"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1512)
                     /gene="sprA"
                     /locus_tag="PK976_06140"
     CDS             complement(<1..>1512)
                     /gene="sprA"
                     /locus_tag="PK976_06140"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HRC67502.1"
                     /translation="EGFYKSSVSSEISLNALNIPQGSVKVTHGGIPLTENVDYTVDYT
                     IGRVKIINEALLSSGTPINISLESNTMFSIQSKTYLGTHIDYEISKDFILGATIINLS
                     EKPLTQKTILGEDPISNTIWGLNVAYQKESLWLTRLLDKLPLYSTSVPSKIAFTGEFA
                     HFIPGHSSAIGSTGTSYIDDFEAAKSTIDLRLPNNWFLASTPQGQTAPGMFPEAALGT
                     GLAYGFNRAAMSWYIIDPIFYDRTGTVRPKNVTKDELSRNLVRQVLENEVFPAKEIPN
                     GVPTNIAILNLDFYPSERGPYNYDVTGVPGFSHGIDAEGFLRNPNSRWGGMMRKIEST
                     DFEAANVEYIEFWMMDPFSEDPDNPGGEIYFNLGDISEDILRDGKKSFENGLPVGPEV
                     KDVDTTIWGRVPKKQSLVNAFDNDPQSRQYQDVGYDGLSDEDERSFFDEVFLQQIVNL
                     YGAGSQAYLKAYEDPSSDNFRYFRGAAMDNDEQYKSVQARYKRYQGPDGNSPTA"
BASE COUNT          457 a          339 c          290 g          426 t
ORIGIN      
        1 ggccgtgggt gaatttccat ccgggccttg atatcgttta tatcgtgctt gaacactctt
       61 atactgttca tcattgtcca tagcagcacc tctaaaatat cggaaattat cggaggaagg
      121 atcctcataa gctttcaaat aagcctgaga gccagcccca taaaggttga cgatttgttg
      181 taaaaatacc tcatcgaaaa acgagcgttc atcctcatca gaaaggccat catagccaac
      241 atcctgatat tgacggctct gtggatcatt gtcgaaggcg ttgaccaaag attgcttctt
      301 aggaacacga ccccaaatag tagtgtctac atcttttacc tcaggcccaa caggaagccc
      361 attttcgaaa ctcttcttac catctctcaa aatatcttcc gaaatatcgc ccaaattgaa
      421 ataaatttcc cctccgggat tatcagggtc ttcggagaaa ggatccatca tccagaattc
      481 aatatattct acattagcag cttcgaaatc ggtagattct atttttcgca tcattcctcc
      541 ccaacggctg ttaggattgc gtaaaaaccc ttcggcatct atgccgtgcg aaaaacccgg
      601 aactccagtt acatcataat tataaggtcc tcgctccgaa ggataaaaat caagattcaa
      661 aattgcaata tttgtaggta caccattggg aatttccttg gcgggaaaaa cttcattttc
      721 gagtacctgc ctgaccaaat tgcgcgaaag ttcatccttc gtaacatttt tcggcctaac
      781 cgttcccgta cgatcgtaaa aaattggatc gataatatac cacgacatgg ctgcacgatt
      841 gaaaccataa gccaaacccg ttcctaatgc agcttcggga aacattccgg gagcagtttg
      901 cccctgtgga gtactggcca aaaaccagtt gttaggcaaa cgaagatcaa tggtcgattt
      961 agcagcttcg aaatcatcga tataagaagt tcctgttgag ccaatggcac tcgaatgccc
     1021 tggaataaaa tgggcaaact caccagtaaa ggctatcttg gaaggtacac tggtcgaata
     1081 taacggaagc ttgtcgagta aacgcgtcaa ccataatgat tctttttgat aagccacatt
     1141 caaaccccat attgtattcg aaattgggtc ttctcccagg atagtttttt gggttaaggg
     1201 tttttccgat aaattgataa ttgtagctcc aagaatgaaa tctttactaa tctcataatc
     1261 aatatgagta cccagataag ttttgctctg gatgctgaac atggtgttgc tttcgagcga
     1321 gatgttgatg ggtgtacccg aactcaacaa agcttcattg ataattttta cccgtccaat
     1381 agtataatct acggtataat cgacattttc agtcaaagga ataccaccat gagttacttt
     1441 gaccgaacct tgaggaatat taagtgcatt aagcgaaatt tcactactta ctgaggattt
     1501 ataaaaaccc tc
//
LOCUS       DAORPD010000491         1509 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_16424_length_1509_cov_2.233150, whole genome shotgun sequence.
ACCESSION   DAORPD010000491 DAORPD010000000
VERSION     DAORPD010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16424_length_1509_cov_2.233150"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1509)
                     /locus_tag="PK976_06145"
     CDS             complement(<1..>1509)
                     /locus_tag="PK976_06145"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRC67503.1"
                     /translation="NPEVDLIEDSPILRALDSLANIKFFQDYYFTADASLTNVYQFPG
                     DFIPVYSDSVYQQRIENMKQNSPIDYAFNRYVKDYIELYAVKKRQLTQRLLGLSYIYF
                     PLFEGMLDKYELPLELKYLAVIESALNPIAGSRAGAKGLWQFMYGTGKVYGLKATSLV
                     DDRFDPYLSTDAACRHLKDLYDLYEDWFLVLAAYNSGVGNVNKAIRRAGGTKNYWAIW
                     PYLPAETRGYVPAFIAASYIMQYAPEHNLYPLHPGILYNGIDTVVVREPVSFDQISEV
                     LGVPIEDIKFLNPSYKLGIIPVAKDQKYYLQLPREFIGDFINNEYTIYNYTTTKGIEK
                     EKLLKQIKNIHEQQIHIVRSGESLSSIARKYHCSVSDIKKWNNLKSNTVKAGKRLIIY
                     SGTSSIASTSSSNNNQTTDTKPEISNSSTNKEQHPNYHIVKRGETLSQISIKYNCTIN
                     ELISWNNLKGYNIQVNQRLLIHPPAAKNDSASSEINKQVKYRYYTVKEGDTL"
BASE COUNT          441 a          298 c          284 g          486 t
ORIGIN      
        1 aaagtgtatc cccttccttc actgtgtaat atctatactt tacctgtttg tttatctcag
       61 aagaagctga atcattcttt gccgcaggag gatgaatcaa tagtctttga ttcacctgaa
      121 tattatatcc ctttaaatta ttccagctta tcagctcatt gatggtacaa ttatatttta
      181 tactaatctg agaaagggtt tctccccttt taacaatgtg gtagttcgga tgttgttctt
      241 tattcgtcga agaattcgat atttcaggct tggtatcggt tgtttgattg ttattcgagg
      301 aactagtaga ggctatcgat gaggtaccgg aataaataat gagtcgttta ccggctttca
      361 cggtatttga tttcaagtta ttccattttt taatatcact taccgaacaa tggtatttgc
      421 gagcaataga cgataaactt tctcctgacc gcacaatatg aatctgctgt tcgtgtatat
      481 ttttaatttg tttgaggagt ttttcctttt ctattccttt tgtagtagta taattgtaga
      541 tagtgtattc attgtttata aaatctccga taaactcacg gggtaactgc aagtaatatt
      601 tctgatcttt tgctacggga atgataccta acttgtagga tggattcaga aatttgatat
      661 cctcgatggg aacgcccaat acttcactaa tctgatcgaa tgagaccggt tcgcgaacaa
      721 cgactgtatc aataccgttg tataagattc cgggatgaag agggtagaga ttgtgttcgg
      781 gagcatactg cataatataa gaggctgcaa tgaaagccgg aacataccct cgggtttcgg
      841 ctggaagata tggccagatg gcccagtaat tttttgtccc tcctgccctt ctgattgcct
      901 tgttaacatt accaactcca gaattatagg ctgccagaac caaaaaccag tcttcataga
      961 gatcgtacaa atcttttaaa tgtcggcaag ctgcatcagt cgataaatat ggatcaaaac
     1021 gatcgtcaac caatgaagta gctttcagac catatacttt ccccgttcca tacataaatt
     1081 gccataatcc ttttgccccc gctcgtgaac cggctatagg attaagagct gactctatca
     1141 ccgctaagta tttcagttcg agaggcagct catacttgtc gagcattccc tcaaataacg
     1201 gaaaataaat atacgacagc cccaataaac gctgcgttaa ctgtcgtttt ttaaccgcat
     1261 acagttcgat ataatccttt acataacggt taaaggcata atctatgggt gaattctgct
     1321 tcatgttctc aattctctgc tgataaaccg aatccgaata tactggtata aaatctccgg
     1381 gaaactgata tacattggtt aaactcgcat cagcagtaaa ataataatcc tgaaaaaatt
     1441 taatattggc taagctatcg agcgcacgta aaatcggtga atcctctata aggtcaactt
     1501 cgggattaa
//
LOCUS       DAORPD010000492         1504 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_16514_length_1504_cov_2.189096, whole genome shotgun sequence.
ACCESSION   DAORPD010000492 DAORPD010000000
VERSION     DAORPD010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16514_length_1504_cov_2.189096"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="PK976_06150"
     CDS             complement(<1..460)
                     /locus_tag="PK976_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67504.1"
                     /translation="MKVSGLTIVRNAVQFDYPVVEAILSILPLCDEFIVGVGNSQDNT
                     LELVQQIASPKIRIFQSIWDENLRQGGRVLAVETDKAFQQISPDTDWCFYIQADEILH
                     ENDLDKVYENMRKNLSDRNVEGLLFRYLHFYGSYDYIGDSRKWYRHEVRIV"
     gene            complement(457..>1504)
                     /gene="purB"
                     /locus_tag="PK976_06155"
     CDS             complement(457..>1504)
                     /gene="purB"
                     /locus_tag="PK976_06155"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004338714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HRC67505.1"
                     /translation="DRLGFAEYKEFIHFGLTSQDINNTALPLALKEAISNVIRQPLID
                     ILSQLHTAARSWKNIPMLSHTHGQPASPTTLGKEIFVFVERLEKQITHLDYQSISAKF
                     GGANGNLNAHKVAYPEIDWITFANSFVNDVLGLERQQTTTQIEHYDDLASLFDNFARI
                     NTILIDLCRDLWLYISMGYFLQKINEQEVGSSTMPHKINPIDFENAEGNLGLANSLFE
                     HLARKLPISRLQRDLTDSTVTRNLGVPLAHSLIAYHSILKGFDKIYPNETKLKDDLDT
                     NWAVITEAIQTILRREGYPDAYETLKKLSRTEKPIDEQTIKEFIDSLEIAEPVKAELR
                     QITPHNYIGIISFE"
BASE COUNT          402 a          290 c          352 g          460 t
ORIGIN      
        1 tgacaattct cacttcgtgc ctataccact tccgtgaatc tcctatataa tcgtaggatc
       61 cgtaaaaatg gagatatcgg aacaaaagcc cctccacgtt tctatcggat aaatttttcc
      121 gcatgttttc gtaaacttta tcaagatcat tttcgtgcag aatttcatcg gcctgaatat
      181 aaaaacacca gtcagtatca ggactgattt gctggaaagc cttatcggtt tccacggcca
      241 atactcgccc tccctgacgt aagttttcat cccagatgct ttgaaatatt ctgattttag
      301 gtgaagctat ttgttgaacc aattcgagcg tattatcctg gctattgcca acacctacaa
      361 taaactcgtc gcatagaggt aagattgaaa gtatggcttc tactacggga tagtcgaatt
      421 gtacggcatt tctgacaatg gtgagtccgc tgactttcat tcgaatgaaa tgattccgat
      481 atagttatgg ggagtaattt gtcgcagttc ggcttttacc ggttcggcta tttcgagcga
      541 atcgatgaat tccttgatgg tctgttcatc gatgggtttc tcggtacggc tgagtttttt
      601 caaggtttca taagcatcag gatatccttc gcggcgtaat atggtttgaa tggcctcggt
      661 aatcacagcc cagttggtat cgaggtcatc cttcaatttt gtttcgttgg gataaatctt
      721 atcgaatccc ttgaggatgg aatggtaagc aatgagtgaa tgtgccagtg gaacgcccag
      781 atttcgggta actgttgaat cggtgagatc gcgctgaagc cgtgagattg gcagtttacg
      841 agccagatgt tcaaatagcg aattggccaa tcccaaattg ccttcggcat tttcgaaatc
      901 tatcggattg attttatgag gcatggttga cgagcctact tcctgttcat ttattttctg
      961 aagaaaatat cccatcgaaa tatacaacca gagatcacga cataaatcaa taagtattgt
     1021 attgattcgg gcaaaattat cgaaaagaga ggccagatca tcatagtgtt ctatttgtgt
     1081 agtggtttgc tggcgttcga gcccaagaac atcattcacg aaactgttgg caaaagttat
     1141 ccagtcgatt tcgggataag ccaccttgtg ggcgttaagg ttaccgttgg cgccgccgaa
     1201 ttttgcagaa atagattgat aatcgagatg agtgatttgt ttttcgagtc gttcgacaaa
     1261 tacaaagatt tctttgccca gtgtggtagg tgaagctggc tgaccgtgag tgtgtgaaag
     1321 catgggaatg tttttccagc ttcgggctgc cgtatgtagt tgagataaga tatctatcaa
     1381 aggttgtctg atgacattgg aaatggcttc tttcagagca agaggaagag cggtattgtt
     1441 gatatcctga gaggtaagcc caaagtgaat gaattcctta tattcggcaa aacccaatcg
     1501 gtcg
//
LOCUS       DAORPD010000493         1503 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_16526_length_1503_cov_2.473066, whole genome shotgun sequence.
ACCESSION   DAORPD010000493 DAORPD010000000
VERSION     DAORPD010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16526_length_1503_cov_2.473066"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..1342
                     /locus_tag="PK976_06160"
     CDS             113..1342
                     /locus_tag="PK976_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HRC67506.1"
                     /translation="MAPVGSYESLMAAIQGGANAVYFGAGKHNMRSRSSVNFTLSDLN
                     DISRICKEKGIRSYLTLNSIIYDEELQEMQNIIDTARESGVSAIIASDPAVINYAVEK
                     GIEVHLSTQCNVTNIEAVKWYSRYADVVVLARELNLQQIKQIVDAIKTYDIRGPSGDL
                     VAIEVFVYGALCMAISGKCYLSLHTMNSSANKGACLQICRRRYDVTDKETGTVLEVDN
                     EYIMSPKDLCTIGILDQILAAGVKILKIEGRGRSPEYVKTVTQVYHEGVEAVLNKTFT
                     QEKVAKWTERLRTVYNRGFWEGYYLGRELGEWTQQHGSQATIKKQYLGKVLNYFSKLG
                     VAEVKLETNDLKIGDKYSINGPTSGVVEGTIEEIRLEYDKIPLAPKGRICSFKVSAPV
                     RRGDKVFLLIENPQSLI"
BASE COUNT          488 a          261 c          320 g          434 t
ORIGIN      
        1 cgaatttatg tatattaaga agttttggaa tggttacagg gcaaatttat gtacctttgc
       61 accctgtaac cattaaaaca tcaagaagtt gagcgaaaaa gaatttgaaa taatggcgcc
      121 tgtcggttca tacgaatcat tgatggctgc tattcaggga ggtgccaatg cggtatattt
      181 tggggctggc aagcataata tgcgctcgag gtcttctgtc aattttactt tatccgattt
      241 gaatgatatt tcccgaattt gcaaagagaa aggcatacgt tcgtatttaa ccttaaactc
      301 aatcatttat gatgaagaat tgcaagaaat gcaaaacata atagatacgg ccagggaatc
      361 aggtgtttcg gccattatag cttccgatcc tgccgttata aattatgccg ttgaaaaagg
      421 aatagaggtt catctttcta ctcaatgtaa tgttaccaat atagaagccg taaaatggta
      481 ctcgcgttat gccgatgtag ttgtgctggc cagagaactg aatctccagc aaataaagca
      541 aattgtagat gcaataaaaa cgtatgatat tcgtggtcca tcgggagacc tcgttgccat
      601 tgaagttttt gtatatggag ccttatgtat ggccatatca ggcaaatgtt atctgagctt
      661 acatacaatg aattcatcgg ccaataaggg agcatgtctg caaatatgcc gtcgtagata
      721 tgatgttacc gacaaggaaa ccggaacagt tcttgaggtt gataatgaat acatcatgtc
      781 gccaaaagat ttatgcacca taggaatttt agatcaaatt ttggctgcag gagtcaaaat
      841 tttgaaaatt gaaggtcgtg gcagatcacc cgaatatgtc aaaacagtca ctcaggtata
      901 tcatgaagga gtagaggcag ttttgaataa aacctttaca caggaaaaag tagccaaatg
      961 gactgaacgt ttgcgcaccg tttataatcg tggtttttgg gaagggtact atctgggcag
     1021 agaactgggt gaatggactc aacaacacgg ttcgcaggct actataaaga aacaatatct
     1081 tggcaaagtg ctcaattatt tctctaaatt aggtgtagct gaagtaaaac tcgaaacaaa
     1141 cgatttgaag attggcgata aatattctat caatggtcct acttcaggag tagtagaagg
     1201 tacgattgag gaaattaggc tcgagtacga taaaatccct cttgcaccga aaggccggat
     1261 atgttccttc aaggtaagtg ctcccgtaag aagaggtgat aaggtctttc tgctgataga
     1321 aaatccacaa tcacttattt aaacaaataa agcagaaata gcgcaatccc caaaataatc
     1381 aaaaaataaa tccaatacga agtttcgctc atttcccctt tcgctttgct tgctttttta
     1441 cccgatgctg gagtaacatt tttgtttgta tagttcttcg atgtttttga cataaatata
     1501 aca
//
LOCUS       DAORPD010000494         1501 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_16564_length_1501_cov_2.635546, whole genome shotgun sequence.
ACCESSION   DAORPD010000494 DAORPD010000000
VERSION     DAORPD010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16564_length_1501_cov_2.635546"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1009)
                     /gene="rmuC"
                     /locus_tag="PK976_06165"
     CDS             complement(<1..1009)
                     /gene="rmuC"
                     /locus_tag="PK976_06165"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HRC67507.1"
                     /translation="MGINIIDIFLTGLGVLIGVLVTGFYFIRKQKKLREGFIEKQKEE
                     ARLQSELDIRNRWYEEIKQEYEKRMQSLSAELDSSRIEASNLKQNLVSLQEKNAYLTQ
                     MKDEAQALYEKFKAEFEALNTKFLKENAEALGEESRKTVKELLDPLKERLETFVRKIS
                     ETSALQSEKQGELKGIIEKLFEQNRNLSEEADRLAKAIKGDVKKLGNWGELVLERVLE
                     ISGLEKEREYVMQWQSSNIEGTALRPDAIILLPDNKQIIIDSKVSLSAFDQYLRAETE
                     DEASLHAQKHVVAVKTHIQNLSSKQYQNLNDLHSTDFVLMFIPIEPALALALSTEPDL
                     YS"
     gene            1053..>1501
                     /locus_tag="PK976_06170"
     CDS             1053..>1501
                     /locus_tag="PK976_06170"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRC67508.1"
                     /translation="MKSFRWLSLLTSLFFVWGKFLFAQQATPAMGKTELREFIKNQMI
                     YPEQALSLGLYGKVELLCVIDADGNLIKSRSIEMTDKDLIREGKRLARGIVWNPARER
                     NKPTEGEYILEIEFHPKKYSKWVKHRGWDRPDADWDTSMQIFSYRNLT"
BASE COUNT          431 a          300 c          293 g          477 t
ORIGIN      
        1 aagaatagag atcaggctcg gtggagagag ccagtgcaag agcgggttcg atgggaataa
       61 acatcaacac aaaatctgtc gaatgcaaat cattcaaatt ttggtattgt tttgagctta
      121 agttctgaat atgtgttttt acggccacaa catgtttctg ggcatgaagg gatgcttcat
      181 cctcggtttc ggcacgtaga tattgatcga aagcagaaag cgaaacttta ctatctataa
      241 taatttgttt attatcagga agcaggataa tggcatcagg ccgtaaggca gtgccttcta
      301 tattggacga ttgccattgc ataacatatt ctctctcttt ttcgagaccc gaaatttcca
      361 ggaccctttc taaaaccagt tcaccccaat tccccaattt ttttacatca cctttgatag
      421 ccttggccag tcggtcggct tcttcactca aatttctgtt ttgctcgaag agtttttcga
      481 taattccttt cagctctccc tgtttttcac tttgcagggc agaggtttcc gaaatcttcc
      541 gaacaaaggt ttccaaacgt tctttaagtg ggtccaaaag ttccttgacc gttttgcggc
      601 tttcttcgcc aagagcttcg gcgttttctt ttaaaaattt cgtattcagt gcttcaaatt
      661 ctgctttaaa cttttcatat aaagcctgtg cttcatcctt catttgtgtt aaatacgcat
      721 tcttctcctg taaactcacc aagttttgct tcagattgct tgcctcaatt cgggatgaat
      781 ccaattcagc cgaaagagat tgcattcgtt tttcatactc ctgcttaatt tcttcatacc
      841 aacgatttct tatatcgagt tcggattgta aacgcgcctc ctccttttgc ttttcgataa
      901 aaccttctct taacttcttt tgtttgcgga tgaaataaaa tcccgtaact aaaacgccaa
      961 tcagcacacc aaggcctgtt aaaaatatat cgataatatt tattcccatc acaaaaattg
     1021 actaatattg taagaggtaa aattaaataa aaatgaaatc atttcggtgg ttaagtttac
     1081 tgacaagcct tttttttgtt tggggcaagt ttttatttgc gcagcaagcc acaccagcga
     1141 tgggcaaaac agaattaagg gaattcatta aaaatcaaat gatttatccc gaacaggctc
     1201 tttcgttggg cttgtatggg aaagtggaat tgctttgtgt cattgatgcc gacgggaatc
     1261 tgataaaatc ccggtctatc gaaatgactg ataaagacct tataagagaa gggaagaggt
     1321 tggcaagagg aatagtttgg aatccagcgc gtgaaagaaa taaaccgact gaaggagaat
     1381 atattttaga gattgaattt catccaaaga aatattctaa atgggtgaaa catcgagggt
     1441 gggacaggcc cgatgccgat tgggacactt ccatgcagat tttttcttac agaaacctca
     1501 c
//
LOCUS       DAORPD010000495         1500 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_16582_length_1500_cov_2.999308, whole genome shotgun sequence.
ACCESSION   DAORPD010000495 DAORPD010000000
VERSION     DAORPD010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16582_length_1500_cov_2.999308"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="PK976_06175"
     CDS             complement(<1..388)
                     /locus_tag="PK976_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008150013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HRC67509.1"
                     /translation="MKLSQFKFYLPQELIANYPADDPDAARMMVLNRKTQTIEHKQFK
                     DVIDYFDEGDVFVINNTKVFPARLYGEKEKTGAKIEVFLLRELNRETRLWDVLVDPAR
                     KIRIGNKLYFGKNDELVAEVIDNTTSR"
     gene            794..>1500
                     /locus_tag="PK976_06180"
     CDS             794..>1500
                     /locus_tag="PK976_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67510.1"
                     /translation="MSLVLILSTRLIMGQITITYHDFPPIPYSTWEYVPTTLENTDIS
                     PNGANQIWDLSGLIAADSTPIQISDLANLPFAGQYPEGNLVFQKNNNYYILSRTPDKI
                     GIIGAVMPMDTALLLSIIYITPELIYTYPYTMGSIVESHPQSLTKIPYNDTISGYFVD
                     SLAINIRASVKHEVVGWGIMKLPYADIKVLKTLTNKTENTTILAYIMGTWFEVDSSIN
                     VYNSIEFQMHNLLYPLVT"
BASE COUNT          466 a          314 c          230 g          490 t
ORIGIN      
        1 ccctcgaagt agtattatcg atgacttcgg caaccagctc atcattttta ccaaaataga
       61 gcttgttgcc aatcctgatt tttcgagccg gatcgactaa aacatcccaa agacgagtct
      121 cacgattgag ttctctcaac aaaaacactt caatctttgc tcccgttttt tccttctcgc
      181 catacaatct ggcgggaaaa accttggtgt tgttgatcac aaaaacatca ccctcatcga
      241 aataatctat tacatctttg aattgcttat gctcgatggt ctgtgtcttt cgattcaata
      301 ccatcattcg cgctgcatca ggatcatcgg cagggtagtt tgcaatgagc tcttgtggta
      361 aataaaattt aaactgcgaa agtttcattg gttattccaa aattgattaa tctgtttgaa
      421 ttcttacgat aaaagatttc ttcttcgatg ataactccta tcaaatgact ttgtgataaa
      481 tcttaagcaa ttttcctgaa attcaatcat ctgaagaaat ggttgctatc gattattttc
      541 tcatcatcaa aaaaatttta aatcattgat tatgattata atggtttgca aaattacaac
      601 aattcgaggc ctttgtcaag agaaatttat aatttctttc gaaagaagaa aattattctc
      661 tatttcaaga gaaatctcca ttcatttatg acgatttctt tccaacaata tgttgaaatg
      721 gcctcattta tgattaaatt tgtatcatct ttcctaaaat tcaaaaccag ccatgaaaac
      781 taaaattctt ctgatgtcat tagtccttat attaagcaca aggctcataa tgggacagat
      841 tacgattact taccacgatt tccctcctat tccctattct acctgggaat acgtgccgac
      901 tactctcgaa aatactgata tttctcctaa tggcgccaat caaatctggg atctgtcggg
      961 tttgattgca gccgattcta ctccgattca aatctcagat ttagctaacc tgcctttcgc
     1021 aggccaatat cctgaaggaa atctggtttt tcaaaaaaat aataattatt acattctttc
     1081 gcgaactccc gataaaattg gcattatagg agctgtaatg cctatggata cagcattatt
     1141 actttctatc atctatatta cacccgaact catctacacc tatccttata cgatgggttc
     1201 aatcgttgaa agtcatccgc aaagcctgac caaaattcct tacaatgata ccatttctgg
     1261 ttattttgtc gatagccttg caatcaacat tagggccagc gtaaaacatg aagtcgttgg
     1321 atggggaatc atgaaactac cttacgccga tattaaggtt ttaaagacac tgaccaataa
     1381 aaccgaaaat actaccatat tggcatatat tatgggaacc tggttcgagg tagatagcag
     1441 catcaatgtt tacaattcca tagaattcca aatgcataat ctgctctatc ctcttgtaac
//
LOCUS       DAORPD010000496         1498 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_16611_length_1498_cov_2.595981, whole genome shotgun sequence.
ACCESSION   DAORPD010000496 DAORPD010000000
VERSION     DAORPD010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1498
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16611_length_1498_cov_2.595981"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1498)
                     /locus_tag="PK976_06185"
     CDS             complement(<1..>1498)
                     /locus_tag="PK976_06185"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HRC67511.1"
                     /translation="PALYPKGLSNLSWIPETDFYAFTNEKALIKKEATKETSDTLIFL
                     QQFNQLLVQNNEKAGKGFPRMTWINKNEFRFQKGNKFYLCNPESQRIVLLTQIPENAG
                     NVDISESSQHIAYTLDNNLYINLRGEQRIISDEKNSGILYGSERVYRNEWGISKGTFW
                     SPDGTKLAFYRMDETMVTDYPIIDITSRPAKVNNIKYPMAGMPIHHVTVGVYNILTQK
                     TVYLNTPEPSAHYLTNITWSPDNKSIYIAVLNREQNHLWLNCYDVSTGNLKRILFEET
                     SPRYVEPENGPWFMPANPAQFVWQSERDGYNHLYLYDTSGNLIKQLTKGNWEVQEISG
                     YSQKLHKFIILSTKDSPLESNIFTVDIRNNEVRRINEEGGSHSVKFNSYGDCFLDIYS
                     NSEVPLKIRLNKLNAKIDKIIFTAENPLKDYKLGETTVFTIKNDDNDDLYCRMITPPD
                     FDTSKIYPVIVYVYGGPHNQLIRNSWLSGAGIYLNYLSQRGYIVFSLDN"
BASE COUNT          425 a          290 c          278 g          505 t
ORIGIN      
        1 ggttgtccaa gctgaaaaca atataacccc tttggctgag atagtttaaa taaatgcccg
       61 ctccgctcaa ccacgaattc ctaattaact gattatgcgg gccaccataa acataaacaa
      121 ttacaggata tattttcgaa gtgtcaaaat cgggaggtgt aatcattcgg caatataaat
      181 catcattatc gtcattctta atcgtaaata cagtggtttc ccctagttta taatctttca
      241 aaggattctc ggccgtaaat atgatcttgt caattttagc attcagctta ttgagtctta
      301 tcttcaaagg aacttcgcta ttagaataaa tatctaaaaa acaatcgcca tagctgttga
      361 attttaccga gtggctccca ccttcttcat ttatgcgtct tacctcatta ttacgtatat
      421 ctactgtaaa aatattgctt tcgagtgggc tgtcctttgt ggaaaggata ataaatttgt
      481 gtaatttttg tgaatatcct gatatttcct gcacctccca attacctttt gtaagctgtt
      541 tgataagatt tcccgatgta tcgtataaat aaagatggtt atatccatct ctttcactct
      601 gccaaacaaa ctgtgcagga tttgccggca tgaaccatgg accattttca ggttctacgt
      661 aacgcggaga ggtttcctcg aaaagtatcc ttttcagatt tccagtagag acatcatagc
      721 aattgagcca caaatgattt tgttcccggt tcagaacagc tatataaatg ctcttattat
      781 cgggcgacca tgtaatattg gttaaataat gagccgatgg ttcgggtgtg tttagataaa
      841 ccgttttttg ggtcagtatg ttgtaaactc ccacagtaac atgatgaata ggcatgcccg
      901 ccataggata tttaatatta ttgacttttg caggcctcga cgtaatatct attatgggat
      961 aatcggtaac catagtttca tccatccggt aaaatgccag tttggttcca tcagggctcc
     1021 agaaagttcc cttactgatt ccccattcat tgcgataaac tctttcactg ccatataaaa
     1081 ttcccgaatt cttttcatca gagatgattc tctgttctcc ccttaaatta atataaagat
     1141 tattatcaag ggtataggct atatgctgag aagattccga tatatcgaca ttgcctgcgt
     1201 tttcgggtat ttgagttaaa agaacaattc tttgggattc aggattacat aaatagaact
     1261 tattcccttt ttgaaatcgg aattcatttt tattgatcca ggtcatgcga ggaaatccct
     1321 tcccggcttt ctcgttgttt tgcaccagaa gctggttgaa ttgctgcaaa aaaatgagag
     1381 tgtcggaagt ttctttggtg gcttcttttt taatcagggc tttttcattc gtgaaagcgt
     1441 aaaaatcagt ttcgggaatc catgatagat tgctcaatcc tttggggtat agtgccgg
//
LOCUS       DAORPD010000497         1498 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_16615_length_1498_cov_2.205128, whole genome shotgun sequence.
ACCESSION   DAORPD010000497 DAORPD010000000
VERSION     DAORPD010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1498
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16615_length_1498_cov_2.205128"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(381..860)
                     /locus_tag="PK976_06190"
     CDS             complement(381..860)
                     /locus_tag="PK976_06190"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HRC67512.1"
                     /translation="MIFKTYLESPIGLLEITGDKNGIHSVYFENKFEKPHNLSLTCQG
                     CPDPLLECRRQLREYFEGWRKTFNLPFILNGTDFQKKVWTQLSKIPYGSLISYSALAS
                     QLNEPMTVRAVAGAVAKNRINIILPCHRVVGAHGHLTGYGGELWRKKWLLGFENPHV"
     gene            complement(887..1255)
                     /locus_tag="PK976_06195"
     CDS             complement(887..1255)
                     /locus_tag="PK976_06195"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRC67513.1"
                     /translation="MHKANISKELRFAVGLVVIFTLSCEKDKPVNPYPNVFSPIASFD
                     YTMNVLENVVRVSFINTSQNADAYLWNFGDGTTSTLVNPVKDYPRPVAKPKSYNVVLT
                     AFDTVNDTYNRTSTTLLFEP"
BASE COUNT          488 a          279 c          271 g          460 t
ORIGIN      
        1 atgagtgttt tacgcataat tttttttgaa caaaattagt agaaaattct aatataatga
       61 tgttcaccct aaaaaatttt tattttaaaa ttgaaatcat tgatagttag ataattatat
      121 ttaaatgatt tttatatgaa tattgaagaa agttcaatat ctattctact ccctgtgtta
      181 aaatgctgtt attatccaaa gtggaatcga ttcaaggata aaaaaatttc gtcagaaacc
      241 gcaggagagt taaaagaaga tatggtgatc taccaaattt attggaataa gcgcatgaaa
      301 aagaactgaa gatttgatag atagcgatcg aaacccaagc tattgtatta cttattttaa
      361 cccattcatt ataattcgag ctaaacatga gggttttcga acccgagcaa ccatttttta
      421 cgccacaatt cgcctccgta acccgtaagg tggccatgtg ctcccactac cctgtggcaa
      481 ggcagaatga tgttgattct attctttgcc acggctccag ccactgcacg cacagtcatg
      541 ggctcattca gctggctggc caaggcggaa tagctgatga ggctgccata cggaattttc
      601 gaaagttggg tccaaacttt tttttgaaaa tccgttccat tcagaataaa gggaagattg
      661 aaggttttgc gccagccttc gaaatattcg cgtaattgac ggcgacattc caataaagga
      721 tcaggacaac cctggcaggt taagctcaaa ttgtggggtt tctcaaattt attttcgaaa
      781 tacaccgaat ggattccatt cttatcgccc gtgatttcta aaagtcctat gggcgattcg
      841 agataagtct tgaaaatcat aaaagattag ctaacaaaag aatacttcag ggttcgaata
      901 gcaaggtggt agatgttcgg ttgtaggtgt cattcaccgt atcgaaagct gtaagcacta
      961 cattataact ctttggtttt gcaactggcc ttggataatc cttcaccggg tttaccaatg
     1021 tgcttgtggt tccatcacca aaattccata aataggcatc ggcattttgc gaggtgttga
     1081 taaaactaac ccttacaaca ttttccaata cattcatagt atagtcgaaa ctggcaatgg
     1141 gactgaatac atttggataa gggttcacgg gtttatcctt ttcacacgat agagtaaaga
     1201 taaccaccaa acccactgca aatcgtaatt cctttgaaat gttcgcctta tgcatatcac
     1261 gaaattttaa ttttctaaac tattttcatc ttcaatcctc acaaattctg agcgatgcat
     1321 aatgttcatt ggcatcataa aaaaaggaag gagtttgcaa atataaccaa agagcaaaca
     1381 tgaacagccg atgtcgaaaa atttccaaat ttctatcgat tatcccaatc aatcttatga
     1441 tgaaatcttt taaaaagatc atgtgcctca tattatatcc attcattttg aaaagtca
//
LOCUS       DAORPD010000498         1497 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_16627_length_1497_cov_2.314840, whole genome shotgun sequence.
ACCESSION   DAORPD010000498 DAORPD010000000
VERSION     DAORPD010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16627_length_1497_cov_2.314840"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1497
                     /locus_tag="PK976_06200"
     CDS             <1..>1497
                     /locus_tag="PK976_06200"
                     /inference="COORDINATES: protein motif:HMM:NF015592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HRC67514.1"
                     /translation="GILNLFETIVDGTVSAELGLKGKPFPDIFITACKNLNVSPNKAV
                     VVEDAVSGVEAGKNGNFGFVLGVARENNHRELLIHGADRVVSDLEELGGIKGMMDWFT
                     NGLSTDNNSLIYYDLVPEKEKQRETLLTVGNGYFATRGSMEETLASICHYPATYMAGV
                     YNRLVSKVAGRDVENEDFVNCINWLPVTFKIGQGEWFDLDKAEIIEIQRELDFRSGIM
                     KRKLTVKFEGKTTRIESERFVSMHQRNIAAQKYTIKPIDYDDEITIKAGLDIDLINDG
                     VDRYRSLNQKHILPLEEKVESNSGYLVGCTTQSNIRIACTYILDANKEGSWQPLVYKG
                     KVFSLFTACIRSNEEVTLTKRIVLNNSLSNDDPLTLVLVEGKNLKSYEDLRELSEVAW
                     RNIWTRVDIELEGDRLAQKIFRLHLFHLFVTASPHNASLDAGIPARGLHGEAYRGHIF
                     WDELFILPLYNLSFPEISRSALMYRYRRLPAAREYARAHGYRGAMFPWQ"
BASE COUNT          426 a          255 c          363 g          453 t
ORIGIN      
        1 ggcattttaa acctattcga aaccattgta gatggaactg tttcggctga attaggatta
       61 aaaggtaaac cttttcccga tatttttatt acggcttgta agaatctgaa tgtttctcca
      121 aataaggcgg tcgttgttga agatgccgtg tcgggggtgg aagcgggcaa aaatggcaac
      181 ttcggatttg ttttaggtgt tgcccgcgaa aataatcatc gtgaattact gattcatggt
      241 gccgataggg tagtaagcga cctcgaagaa ttgggtggaa taaaaggaat gatggattgg
      301 tttaccaatg gtttgtcaac tgataacaat agcctcattt attacgatct ggttcccgag
      361 aaggaaaaac aaagggaaac tcttttgaca gttgggaatg gatattttgc aacccgtggc
      421 tcgatggagg agacgttagc cagtatatgc cattaccctg ctacttatat ggctggtgtt
      481 tataatcggc ttgtttcgaa agtagcggga agagatgtcg aaaatgaaga ttttgtaaac
      541 tgtatcaatt ggctgcctgt cactttcaag attggccaag gcgagtggtt tgatctagat
      601 aaggccgaaa tcatagaaat tcaacgtgag ttagattttc gttcaggtat tatgaaacgt
      661 aagttgacag tcaagtttga agggaaaacc actcgaatcg aatctgaacg ctttgtctcg
      721 atgcaccaga gaaatattgc tgctcagaaa tataccatta aaccaatcga ttatgatgat
      781 gagattacga taaaagcagg gctcgatatc gatttgatca atgatggtgt ggaccgctat
      841 cgttctttga accaaaaaca cattttgcct cttgaagaaa aggtagagag taattcgggt
      901 tatttggtgg gttgcactac tcagagcaat ataaggattg cctgtactta tattttggat
      961 gctaacaaag aagggagttg gcaacctctt gtttataaag gcaaggtttt ttcgttgttt
     1021 actgcctgca taagatctaa tgaagaagtt acgttgacta aacgtattgt attgaataat
     1081 agcctttcga atgatgatcc cctaacttta gttttagtag aaggcaaaaa tttgaaatcg
     1141 tatgaagatt tgcgagaact atcggaagtg gcttggcgaa atatctggac acgggtagat
     1201 attgaactcg aaggtgatcg tttggctcag aaaattttcc gactgcacct atttcattta
     1261 ttcgtaacgg catcacctca taatgcttcg cttgatgctg gtattcccgc acgtgggttg
     1321 catggtgaag cttaccgtgg acatattttt tgggatgaac tgttcatact tccgctctat
     1381 aatttgagtt ttccagaaat cagccgcagt gccttgatgt atcgttatcg tcggttgccg
     1441 gctgcacgag aatatgcccg tgcgcatggc tacagaggtg ccatgtttcc atggcaa
//
LOCUS       DAORPD010000499         1495 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_16664_length_1495_cov_2.146528, whole genome shotgun sequence.
ACCESSION   DAORPD010000499 DAORPD010000000
VERSION     DAORPD010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1495
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16664_length_1495_cov_2.146528"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1038
                     /locus_tag="PK976_06205"
     CDS             <1..1038
                     /locus_tag="PK976_06205"
                     /inference="COORDINATES: protein motif:HMM:NF025714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4403 family protein"
                     /protein_id="HRC67515.1"
                     /translation="LNFSDSRELNTEILLKFSTRVELQPDWTVLTQTQSDGYAWLKSP
                     TMKFGSYDIGITRLANILMQAGQQRLASAVDTLLMQELKIKFYASSLWNAIQQPIKIN
                     ANPVVWLQMKPLGFYALPFHAQNNNIDYRLGISCILTALVNENPPYQDSVSLPALSFD
                     FPQNRESIVHTRVEASMKTAEDLANQMLRGQKFKFGQKEFIVRSIKLYGSESKLAVEA
                     QIGGEINGKLYFTGYPYYDTTTQSIRLRDFQFDLKTDNILKDIASWVKRTNIENSLYK
                     LLVFDLSKQIAFLQDVFGSFLNELSRTNHIEINGKINSFSVDDIQISPEIITAYLTAK
                     GEIGVRLLPSS"
     gene            complement(1074..>1495)
                     /locus_tag="PK976_06210"
     CDS             complement(1074..>1495)
                     /locus_tag="PK976_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018668714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HRC67516.1"
                     /translation="IVTFLVHHYILIPLQITFLQTIAYILVIAALVQMVEIILKKISP
                     SLYQALGIFLPLITTNCAVLGVAILVIQKNFNLMESVVYTIATALGFGLALIIFSGLR
                     EQIELNQVPKGMRGTPIALVTAGLLSLAFMGFTGIVK"
BASE COUNT          471 a          282 c          309 g          433 t
ORIGIN      
        1 cttaattttt ccgattcccg agaattgaat actgaaattc ttttgaagtt ttctactcga
       61 gtagaattac aacccgactg gacagttctt acacaaacgc agtcagatgg ctatgcgtgg
      121 ctaaaatcgc ctacgatgaa atttggcagt tatgatattg gtataacaag gcttgccaac
      181 atcctcatgc aagcgggtca acaaaggctc gcttctgctg tcgatacatt gctcatgcag
      241 gagcttaaaa taaagtttta cgcttcgagc ttatggaatg caatacagca gcctataaaa
      301 attaatgcta atcccgtggt atggctgcaa atgaagccct tagggtttta tgctcttccg
      361 tttcatgcgc aaaacaacaa tattgattac cgattgggaa tttcgtgtat tctaacggct
      421 cttgtaaatg agaatccacc ttatcaagac tcggtttcac ttccagcctt aagtttcgat
      481 tttccccaaa atagagaatc gatagtgcat acacgtgttg aagccagcat gaaaaccgcc
      541 gaagatttgg ctaatcagat gttaagaggg cagaaattca aatttggcca gaaggaattc
      601 atcgttcgat ccatcaaatt atatggaagc gaaagcaagc ttgccgtgga agcgcagatc
      661 ggtggagaaa taaatgggaa actttatttt acgggatatc catattacga taccaccacc
      721 cagtctattc gtcttcgtga ttttcagttc gacctcaaga cggacaatat attgaaagac
      781 attgcttcat gggtaaaacg aacgaacata gaaaattcat tgtataaatt actggttttc
      841 gacttgagta agcaaatagc ttttttgcaa gatgtttttg gttcattcct caatgaactc
      901 tcaagaacga atcatatcga gataaatggg aaaataaatt ctttcagtgt agatgacata
      961 cagatttccc ctgaaatcat cacggcttat ttgacagcaa agggagaaat cggcgttcga
     1021 cttctccctt cttcatagca aaatttggaa tattccgtaa aagtttgaat tttttatttt
     1081 acaatgcccg tgaatcccat aaaggcaagt gaaagcagtc ctgcagtaac aagtgctatt
     1141 ggtgtgcctc tcattccttt gggaacctgg ttgagttcta tttgttcacg taaacccgag
     1201 aagatgatta aagccagtcc gaatcctagg gcggtagcaa ttgtataaac cacactttcc
     1261 atgaggttga aattcttctg gataaccaaa atggccacac ctaatactgc acaattagta
     1321 gtgattaaag gaagaaatat tcctaaagcc tgatagaggg aagggcttat tttcttgagg
     1381 ataatttcta ccatttgaac taaggctgca atgacgagaa tatatgctat ggtttgaagg
     1441 aaagtgattt gcagagggat gaggatataa tggtgcacta gaaaagtaac aatgg
//
LOCUS       DAORPD010000500         1490 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_16749_length_1490_cov_1.917770, whole genome shotgun sequence.
ACCESSION   DAORPD010000500 DAORPD010000000
VERSION     DAORPD010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16749_length_1490_cov_1.917770"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1248)
                     /locus_tag="PK976_06215"
     CDS             complement(<1..1248)
                     /locus_tag="PK976_06215"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRC67517.1"
                     /translation="MNKKTLILINPSNQYRRGFALRQQSRQVPLGLGIIAALTPPNWK
                     IIILDENIKPFRFREADLVGITTFTSTAQRAYEIAAVYRGKGIPVVFGGIHVSMLPDE
                     ALQYGDAVVIGEAETSWPNVMSDFEAGRLQRIYRGPHADLSQCPIPRHDLFHPSYLFA
                     SIQTSRGCPMDCDFCSVPVFNGHSYRLRPVEAILDEMEALPQKLIYFVDDNITGYSKA
                     SEKHAQELFEGMIRRGLKKEWFAQASLNISQNPELLKLAARSGCKMLLIGIEAENENA
                     LRDTNKHVNLTLGTKTYKHAFRTIHQAGIAVLGAFIYGMDSDTPSSLQARTKFIVHSS
                     VDVVQASAITPLPGTRLFKRLQQENRILANHPPHSWKYFHFSDVVFRPAEMQAHELAD
                     NLWMAYQKIFSLKTLRMKFLRTLW"
BASE COUNT          431 a          301 c          335 g          423 t
ORIGIN      
        1 ccagagtgta cgcagaaatt tcattctcaa tgttttcaac gagaaaattt tttggtaagc
       61 catccaaagg ttatcggcaa gttcatgagc ttgcatctct gccggccgaa aaactacgtc
      121 ggaaaaatga aagtatttcc acgaatgggg aggatgattg gccagaatcc tgttttcttg
      181 ctgaagtcgt ttaaaaagac gggttcctgg caatggagtt atggcactgg cctgcaccac
      241 atctaccgag gaatggacta tgaatttggt acgggcttga agcgaggaag gtgtgtcaga
      301 gtccatgcca tagataaagg cgcctaaaac ggctattcct gcctgatgta ttgtccgaaa
      361 agcatgcttg taagttttgg tccctaaggt taaatttaca tgtttatttg tatcacgaag
      421 ggcattttcg ttctcggctt cgataccaat caacaacatt ttacatccgc tgcgcgctgc
      481 cagtttcaaa agttctggat tttgcgaaat gtttaagctt gcctgcgcaa accattcttt
      541 ttttaatcct ctgcgtatca ttccttcgaa cagctcttgt gcgtgctttt cggatgcctt
      601 gctataaccg gtaatgttgt catctacgaa ataaataagt ttttgtggta gagcctccat
      661 ttcatccagt atggcctcta cgggtcgcag ccggtagcta tggccgttga aaaccggtac
      721 cgaacagaaa tcacaatcca tggggcagcc acggctggtt tgaatactgg cgaataggta
      781 agaaggatga aagaggtcgt gtcgtggaat ggggcattgt gaaagatcgg catgtgggcc
      841 gcgatagatt ctttgaagcc gtcccgcttc aaaatcactc ataacgtttg gccatgaagt
      901 ttctgcttcc ccaattacca cggcatcacc atactggaga gcttcatccg gaagcattga
      961 aacatgaatg cctccaaaaa ccactggaat tccctttcca cgataaactg cggcgatttc
     1021 ataggcgcgt tgagcagtgg aagtaaatgt tgtaatgcct acaagatcag cttcgcgaaa
     1081 acgaaatggt ttaatattct catccagaat aataattttc caattaggag gtgttaaagc
     1141 agcaataatc cccaatccga gtggcacctg acgagattgc tggcgcaagg caaatcctcg
     1201 cctgtactga ttggaaggat taatcaagat taaagtcttc ttattcaacg attaaatgaa
     1261 aatattacga ccgctaaaat tacgataatt tataaagatg agtcggtgga gtattttcta
     1321 ataagggagc attggcatgg ctacagcaaa tagtgttgta ttcatccttc attgtgagga
     1381 ataagagtaa tcttgataaa aaccttaaat ttgccaacca atttaatttt ctcacgatgg
     1441 ctttgaatat acatgatttt atagaaagaa tcgaagacga attcgatgaa
//
LOCUS       DAORPD010000501         1488 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_16784_length_1488_cov_1.960921, whole genome shotgun sequence.
ACCESSION   DAORPD010000501 DAORPD010000000
VERSION     DAORPD010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16784_length_1488_cov_1.960921"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1467)
                     /locus_tag="PK976_06220"
     CDS             complement(<1..1467)
                     /locus_tag="PK976_06220"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRC67518.1"
                     /translation="MSLSQIIMIGAWLFYGHYKIGLKQLLNKKIAWVFISFYLLHILG
                     LIYTTDFAYAIKDLRTKLPLLVLPFVIFTMPRPSNKFLQLIVWLHALAIFLASLIGIY
                     LAWQKGYVNYRLYSPGISHIRFGLNICLSAVLLLYDIIHRKKLIPRILASLLIAWYIV
                     YLSLFGSVTAIVILGILLVIGISIFIIRHSPVWGKIIYSFILTASILGFSFLIKETLQ
                     LILHHSKEKIDFANLEKFSADGHAYLHDTVYFGKEFGQYVGLYYCPDELPQAWAKRSK
                     LDYYGKDLAGQPLMATIVRYLNSKGLRKDARGLAQLSDEEIGWIEKGVANAQYMQSNA
                     LKRTIMEFALGYEHFKEGSGTRGNSIIQRFELWRASIEIIKKNPVIGVGTGDVPQAFK
                     QQLMAMHSDLSHTSLRSHNQYLSITIAFGIIGLLWFLWILLYPLKINQLYKDWRYVAF
                     LIIMAVSMINEDTIESQAGITYYVFFSLFLYLLSIEKET"
BASE COUNT          475 a          279 c          301 g          433 t
ORIGIN      
        1 ggtctccttt tctatcgaaa gaagatataa aaataaggaa aaaaatacat aataagtgat
       61 tccggcttgc gactcaatag tatcttcgtt gatcatagaa actgccatga tgattaaaaa
      121 ggccacataa cgccagtctt tgtataattg attgatttta agagggtaga gtagtatcca
      181 tagaaaccat agcaaaccaa taatgccaaa agcaatggta atgcttaaat attgattgtg
      241 cgaacgtaga ctggtatggc tcaagtcgga atgcatagcc atgagctgtt gtttgaatgc
      301 ttgtggaaca tcgccagttc cgactccgat cactggattt tttttgatga tttcgattga
      361 agctcgccag agttcgaatc tttgtataat actatttccc ctggtgccac ttccctcttt
      421 gaaatgttca tatccaaggg caaattccat aatcgtacgc tttagggcat tgctttgcat
      481 atattgagcg ttggccacgc ctttttctat ccatccgatt tcttcgtctg ataactgggc
      541 gagccctcgg gcatccttgc gtaaaccttt gctgttgaga tatcttacga tagtagccat
      601 caagggttgg cctgcaaggt ccttcccata gtaatcgagt ttgctacgtt ttgcccaggc
      661 ttgcggaagt tcgtcggggc aataatataa acccacatac tgcccaaatt ccttcccaaa
      721 gtaaaccgta tcatgtaagt aagcatgccc atcggcactg aacttttcga ggtttgcaaa
      781 gtcgattttt tcttttgaat gatggagtat gagttgcaaa gtttccttta tcaagaatga
      841 aaaacctaat attgaggcgg ttaaaatgaa ggaatagata atttttcccc atacagggct
      901 gtgacgtatt atgaaaatac tgatgcctat caccagaaga atacccagaa taacaatggc
      961 agttactgaa ccaaaaagac taagataaac gatataccat gcaatgagga gggaggctaa
     1021 aattctcgga atcaattttt ttcgatggat gatgtcgtat aatagcaata cagccgaaag
     1081 acaaatgttc aatccaaacc tgatatggga aattcctgga gaatagagcc tgtaatttac
     1141 atatcctttt tgccatgcca agtaaatgcc gatgaggctg gctaagaaaa ttgccaatgc
     1201 atgcaaccag acgataagct gtaaaaattt attcgaaggc ctgggcatgg taaatattac
     1261 aaaaggcaat accaacaaag gcaacttggt tcttaaatcc ttaatggcat aagcaaaatc
     1321 tgtcgtatag attagcccaa gtatatgaag aaggtaaaat gaaatgaata cccaagcaat
     1381 ttttttgttt agtagttgtt ttaagccgat tttataatgt ccatagaata gccatgcacc
     1441 aatcataata atctgggaaa gactcattac ataattggat gtgggtat
//
LOCUS       DAORPD010000502         1487 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_16798_length_1487_cov_2.465782, whole genome shotgun sequence.
ACCESSION   DAORPD010000502 DAORPD010000000
VERSION     DAORPD010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1487
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16798_length_1487_cov_2.465782"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="PK976_06225"
     CDS             complement(<1..392)
                     /locus_tag="PK976_06225"
                     /inference="COORDINATES: protein motif:HMM:NF013838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliG C-terminal domain-containing protein"
                     /protein_id="HRC67519.1"
                     /translation="MIRVEKNSVGKLEYEGWPSLKSVYEFNNISGPPLPFVTQAPHFV
                     RSDKKSSKLIAQPSDLIHFSDRKLKSALKNTDSEFIAKIIANSAEEVTDKILRNLTKQ
                     RGEEVKELAHEIKKVSKKALSEAVEEIL"
     gene            complement(375..1487)
                     /locus_tag="PK976_06230"
                     /pseudo
     CDS             complement(375..1487)
                     /locus_tag="PK976_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598764.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
BASE COUNT          427 a          285 c          298 g          477 t
ORIGIN      
        1 aagaggattt cttcaacagc ctcactcaag gcctttttcg aaaccttttt aatttcatga
       61 gccagttctt taacttcttc tcctctttgt ttggtcagat tacgaagaat tttatcggtt
      121 acttcttcag ccgagttggc tatgattttt gcgataaatt ccgaatcagt attttttagt
      181 gcacttttca gctttcgatc actaaaatga attaaatcgg aaggttgtgc tattaactta
      241 gatgattttt tatcgcttct aacaaaatga ggtgcttgag ttacaaaagg aagaggtggt
      301 ccggaaatat tgttgaattc atatacactt ttcagtgaag gccatccttc atattcgagt
      361 tttccaaccg aattcttttc tactctaata ataaaatcgg ataaaacatt catataatta
      421 atataggctt cgtaaggcga aggataagga ttgaagtatt tgtgaacttc accatcggag
      481 aaccattttg tacacatata atagaaatga tcgctggttt ggagatagag ccaatctttc
      541 cagatggaag ggtcatcgca ggcgtaaact ttagatacca gcgcttagag gttcgaaacg
      601 gcttcttcct gtagttcatt gcccagccag gcagtgaggt ctctttcttc gtcggcccag
      661 gatgtagcat gaggaacgtg aatgggagct actggctgat gtaggctggc aatttcggtt
      721 ggagtatgaa attcgaagtt tgattgggtt agtatggttt tcggcaatgc ttctaaaaat
      781 tcgaaaattc cagtttcttt ccagtgatgt tcaccaaaag tttcataatc gagaaaaata
      841 ttaactaatt cctgattggg atcgaaggca ttgagccaac taacatactt ttcggccgta
      901 agcggccatt ctgaccatgc tttgtttgaa aagcgaaaac caatgtcgtc gcttaatcga
      961 aaattacgta acaggactct aagcctggga ttcaaggcat tgtaatacaa ataatttgga
     1021 ctcctccatc ccagtatatg ttttgcccct tctgccagaa tagagtaaaa acccatttcg
     1081 gctatggttg ctccaatttc atccgaatag ataagctcag tattgcggaa tgaagtaggt
     1141 ttgatcccga aaagttcttc gactctttgt gagtgcatga ggatttgcct tttaaattct
     1201 tccttgtttt ttaagcctgc aagcgaatgt ccataagttt cggctaaaaa ctccacctgc
     1261 ccagttgcag caagctgctt aaaactatcc aaaacctcgg gggtatattt ttcgaactgt
     1321 tctattgcag tcccagaaat cgagaaacaa accctgaaag cttggccatg ccttcgaatg
     1381 agagttaaaa gcaatttatt tgctggtaaa tagcattttt cggcaactct gcgaatgata
     1441 aagcgatttt gatattcatc ataataattt gaatcattac ctatgtc
//
LOCUS       DAORPD010000503         1486 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_16828_length_1486_cov_1.825297, whole genome shotgun sequence.
ACCESSION   DAORPD010000503 DAORPD010000000
VERSION     DAORPD010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16828_length_1486_cov_1.825297"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="PK976_06235"
     CDS             complement(<1..749)
                     /locus_tag="PK976_06235"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRC67520.1"
                     /translation="MNERKNIIITGATGFIGSFLTEIGVEKGWQVTAWVRETSNTEHL
                     PSGVNLIKAGFSNRQKMMEALQMIERQNGSIDYIVHNAGVTKTTHPEDFDRVNAENTG
                     RFIEAIQESGISIAKFILMSSLAALGPGNPQTLEPLHPDDVPKPDTLYGKSKLKAEKI
                     VRQSSLPWNIMRPTGVYGPRETDYYLMLRTIKKGLAPVTGCKPHFLSFVYVKDLVKAV
                     FLALETPITQQTFHVSDGKTYTDREYRDLCC"
     gene            complement(767..>1486)
                     /locus_tag="PK976_06240"
     CDS             complement(767..>1486)
                     /locus_tag="PK976_06240"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF013845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dimethylallyltransferase"
                     /protein_id="HRC67521.1"
                     /translation="PGEEYSVWDFSCDFKKAYLEIRSRGRQVILCGGTGLYIEAGIGG
                     YYMPQVPEDKNLRNSLEKESIENLITRLASLRPLHNTTDITDRARLIRAIEIETWKLS
                     HPREETFVPPNFILGITLPREELRMRITQRLHKRIEQGMIEEVEQLKKLGISHENLDS
                     YGLEYRFISRYLKGQMSKNEMVESLNTAIHQFAKRQMTWFRRMERKGFRIHWLDGMQL
                     AEEVVKKAMQVLDSHPDQWKE"
BASE COUNT          387 a          324 c          289 g          486 t
ORIGIN      
        1 ttgcagcata aatccctata ttcacgatcg gtataggttt taccgtcaga aacatgaaat
       61 gtttgttgtg taataggtgt ttcgagagct aaaaaaacgg cttttaccaa gtcttttaca
      121 tagacaaaag aaaggaaatg aggcttgcag ccagtaactg gagccagtcc tttctttatt
      181 gtgcggagca tgaggtaata atcggtttct cgtgggccat acactcctgt gggtcgcatg
      241 atattccagg gtaaagacga ttggcgaaca attttttcag cctttaactt gctttttcca
      301 tacaaggtat cgggttttgg aacatcatca ggatggagtg gttcgagggt ttggggattg
      361 cctggcccca aggctgccaa actactcatc agaataaatt ttgcaattga aataccactt
      421 tcctgaatag cttcgataaa tctgccggta ttttctgcat tcaccctatc aaagtcctca
      481 ggatgagttg tttttgtaac tccagcatta tgaacgatat aatcgatgga accattctgg
      541 cgctcgatca tctgtaatgc ttccatcatc ttctgtcgat tagaaaaacc cgcttttatg
      601 agatttactc cagaagggag atgttcggta ttacttgttt cccttaccca ggcggttacc
      661 tgccatcctt tttcgacgcc aatttcggta agaaaacttc caataaaacc cgtagctcct
      721 gtaatgataa tattttttct ttcgttcata ttctttgtca ttttaatcat tccttccatt
      781 gatctggatg gctatcgaga acctgcatag cctttttcac gacttcttca gctaattgca
      841 ttccatctaa ccagtgaatc cgaaatccct tgcgttccat ccttctgaac cacgtcattt
      901 gccgtttcgc aaactgatga atagccgtat ttaacgattc taccatttca tttttcgaca
      961 tctgcccttt caggtaacgg cttataaagc gatattccaa tccataggaa tcgagatttt
     1021 cgtgacttat gccaagcttc ttgagctgct ctacttcttc tatcatgcct tgctctattc
     1081 gtttgtgaag tctttgggta atcctcattc gaagctcttc tcggggtaat gtaatgccaa
     1141 gaataaaatt gggaggtacg aaggtttctt ctcggggatg tgaaagtttc caagtttcta
     1201 tttcgatggc acgaattaaa cgagctcggt cggtaatatc ggtagtattg tgtaaaggtc
     1261 gcagggatgc aagccgagtg ataagatttt cgatagattc tttttcgaga gaatttctta
     1321 aatttttatc ctcgggtacc tgaggcatat aataccctcc aatccctgct tcgatatata
     1381 aacctgtacc accgcataga attacctgcc tgcctctact acgaatttcg agataagctt
     1441 tcttgaaatc acatgaaaaa tcccatacac tatattcttc gcctgg
//
LOCUS       DAORPD010000504         1485 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_16839_length_1485_cov_2.981818, whole genome shotgun sequence.
ACCESSION   DAORPD010000504 DAORPD010000000
VERSION     DAORPD010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16839_length_1485_cov_2.981818"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(732..1163)
                     /locus_tag="PK976_06245"
     CDS             complement(732..1163)
                     /locus_tag="PK976_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRC67522.1"
                     /translation="MITEESLRQFGVLIHEVRNVSSDEAVALLSDGAVILDVRPLFES
                     EYRRFDVKDVIYAPAEGYEYWVKNLPFDRCLIVADASGLKSRTIASRLLADGFRMVVN
                     LSGGLVDWERRGAPVIVDKSKQLMGSCLCQLKPFYYKKRRE"
     gene            complement(1304..>1485)
                     /locus_tag="PK976_06250"
     CDS             complement(1304..>1485)
                     /locus_tag="PK976_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67523.1"
                     /translation="NAQHGYGSSLEITFLPKLPVKGLRFNIHKPAIKFMLICDSEILI
                     FAMEILSVNYSPDSV"
BASE COUNT          473 a          279 c          219 g          514 t
ORIGIN      
        1 aagaagttta tattttactg aaaattttga tgactgtatg gacagactct aattatcaaa
       61 atagattatt gaatgctaaa ttaattttta gtggcagagt ttttccctta aaattataaa
      121 ccataaatga gcctctattt acaatttatt tttgaaacaa ttgaccaggt tgttaaaaag
      181 ctattactga aagtttaata ttatctattt gttcatcatg ttttaaatca actgtatata
      241 acatcatatc gttggaatta ttaaattgat aaggtttatg tttgttatta aaagtgtatc
      301 tcttaccttg ccaataattt gagtttttat ttcatcgggt ttatagtcga gcagtttgct
      361 tatgccagaa ccattttatt tataaataaa tacaaaagaa tcgacaattt aaatcacttg
      421 aagaaataaa atttattatt ggaatcataa ataaacttat ttgagtttct ttatggaact
      481 ccgttttatc tccaaaaatc ctaaatgtaa aagaatccgt cacatttaaa aatggttctg
      541 gcaatattga aaaataattt gtgtatttgg tatagaagat cgctcatatc ttgtattttc
      601 caaactctgt tcactattcc cttcacctcg cgtctttttt gacttttgtt cttctcgcgg
      661 ctcatgcctc gaaacaatta aaccataaca atacttgcta aaaaactatc ctgtataata
      721 atgaatttaa actattccct tcttttttta taatagaatg gtttaagctg gcaaaggcag
      781 gaacccataa gttgcttcga tttatccaca attaccggtg cacctcttct ttcccaatct
      841 accaatcctc cactaaggtt taccaccatc cggaatccat cggcaagtaa acgactggca
      901 atggttctgc tttttaggcc actggcgtcg gctacaatta agcaacgatc gaagggcaaa
      961 ttttttaccc aatattcgta tccttcggct ggtgcgtaaa ttacatcctt tacgtcgaat
     1021 ctacgatatt ccgattcgaa caatggcctt acatccagta taacagcccc atcagataga
     1081 agagccactg cctcatccga acttacattc ctcacctcgt gaatcaaaac tccaaattgt
     1141 cgaagacttt cttcagttat catttttcga tgtatatttc aaatcaaaat ttgctgttta
     1201 gctttacaat tgtaaaaatc tttcactcga tatgtataat aatccttcgt tcgggccagc
     1261 tcgtcgaata ctaacttgat gctcttttct tagagcaatt gaattataca gaatctgggc
     1321 tgtaattcac cgaaagaatc tccattgcaa aaataagaat ttcagaatca caaatcagca
     1381 taaatttaat agcaggctta tggatgttga agcgaagccc ttttacaggt agcttgggta
     1441 agaaagtaat ttccagtgaa gaaccgtacc catgctgtgc atttc
//
LOCUS       DAORPD010000505         1485 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_16843_length_1485_cov_2.723776, whole genome shotgun sequence.
ACCESSION   DAORPD010000505 DAORPD010000000
VERSION     DAORPD010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16843_length_1485_cov_2.723776"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..925
                     /locus_tag="PK976_06255"
     CDS             <1..925
                     /locus_tag="PK976_06255"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRC67524.1"
                     /translation="PEMEKTARKKVLGGVAISIAAIMWGLDGIVLTPRLYNLDVAFVV
                     FMLHLIPFILMQFFLFREYTYFKNLSAQSWLALILVALFGGAIGTLAMVKALFLVHFQ
                     KLTIVVLLQKLQPVFAITLATFLLKEKLKPNFFIWAAIAIIAGYFLTFGFQLPTISRD
                     PNMLYAAGFSLLAAFSFGSSTVFSKWALKEVRFETATFIRYGLTALILLPYILLTGRF
                     SELSRVTTLNWILFVIIGLTTGSGAIFLFYFGLRHVKASVSTICELFFPLSAVIFDYL
                     FNHVTLSLVQWISAIVMIFAIVILSLQEKTD"
     gene            complement(1028..>1485)
                     /locus_tag="PK976_06260"
     CDS             complement(1028..>1485)
                     /locus_tag="PK976_06260"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67525.1"
                     /translation="ISESPFVPVSGIFFGDYTNISVHNNIIRPIWTRLHNGQLSIWTD
                     ITPLENLLASTQNTYPTKENGTVEGYPNPSKGIFYVSFKLHAPALVSLDIYDQNGRLA
                     HSIFSQCQFDYGKHIIPIDTKKIGLKPGSYICSLKSKEWKEKQIVMIVL"
BASE COUNT          387 a          258 c          298 g          542 t
ORIGIN      
        1 gccagaaatg gagaaaacag cccgaaaaaa agttttagga ggagttgcca tcagcatagc
       61 tgctatcatg tggggattgg atggaattgt gctaactcct cgcctttata atctagatgt
      121 ggcttttgta gttttcatgc ttcatctgat tccttttatt ctcatgcaat tttttttatt
      181 tcgcgaatat acatacttta agaatctttc agctcaaagt tggcttgctc ttattttggt
      241 agctttgttt ggaggggcta ttggcacact ggctatggtg aaagctttat ttctggttca
      301 ttttcagaaa ctaactatag tagtgctatt acaaaagtta cagcctgttt ttgctattac
      361 cttagctacc ttcttactga aagagaaact taaaccgaat ttttttatct gggcagcaat
      421 tgctattata gcgggttatt ttttaacatt tggtttccaa ttgcctacaa taagtaggga
      481 tcctaatatg ctctatgccg ctgggttttc tttgctagct gcattttcat ttggcagcag
      541 cacagtattt agtaaatggg cacttaaaga agttcgtttc gaaaccgcta cttttatccg
      601 ttatggatta acagccctca ttcttcttcc ttatattctg ttaacaggtc gcttctctga
      661 actttccaga gttacaaccc tcaattggat tcttttcgtc atcattggac tgacaacggg
      721 ttcgggagct atcttcctct tctattttgg cttgcgtcat gtaaaggctt ccgtatctac
      781 tatatgtgag ttgtttttcc ctctatcggc agttattttc gattatttat ttaatcacgt
      841 tactctttca cttgttcaat ggattagtgc cattgtcatg atttttgcca ttgtcatact
      901 cagcctgcaa gaaaaaacag attaaaaaat ctgtattaaa attgcgataa caaaaaataa
      961 ttttattgga tatttttctc ctattaattt tatgccgaat ctagagttct ggagttattg
     1021 gtggttttta aagcacaatc atcacaattt gtttttcttt ccattccttc gattttaatg
     1081 aacaaatgta tgagccaggt ttaaggccga tttttttggt gtcaatagga atgatatgtt
     1141 tgccataatc gaattggcat tgggaaaaaa tggagtgggc gaggcgacca ttttggtcgt
     1201 aaatgtcgag tgaaaccaat gcgggagcat gaagtttgaa actaacataa aaaattccct
     1261 tgcttggatt aggataaccc tctactgttc cattttcttt tgttgggtag gtgttttgtg
     1321 ttgaggcaag aaggttttca aggggagtaa tatcggtcca gatgctgagt tgtccattgt
     1381 ggagtcgagt ccagataggt ctaatgatat tattatgtac cgaaatgttg gtataatctc
     1441 cgaagaagat tcccgaaacg ggtacaaaag ggctttcgct tattt
//
LOCUS       DAORPD010000506         1485 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_16850_length_1485_cov_2.009790, whole genome shotgun sequence.
ACCESSION   DAORPD010000506 DAORPD010000000
VERSION     DAORPD010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16850_length_1485_cov_2.009790"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            471..1334
                     /locus_tag="PK976_06265"
     CDS             471..1334
                     /locus_tag="PK976_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster domain-containing protein"
                     /protein_id="HRC67526.1"
                     /translation="MNPSILYAVITLAVIGAGSGAILYWVASKFKVEEDPRIDTVAEA
                     LPQANCGGCGYAGCRNFAEAIVKTGSLDNLLCPVGGDEVMKRIASILGLEAPTMQPMI
                     AVVRCNGSFQNSPSKFHYDGVKSCAFAHITFTGEGGCPFGCLGQGDCERACSFDAIHI
                     NPKTGLPEVNDKCTACGACVKACPRNIIEMRPKGKKDRRIFITCMNQEKGAIARKNCE
                     AACIGCNKCVKECRFDAITVSNNLAYIDPHKCTLCRKCVEVCPTNCILEVNFPPRKRT
                     TQSDSPAEITA"
     gene            1369..>1485
                     /locus_tag="PK976_06270"
     CDS             1369..>1485
                     /locus_tag="PK976_06270"
                     /inference="COORDINATES: protein motif:HMM:NF024768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67527.1"
                     /translation="MLKTFKKGGVHPHENKFSANSPIQPIDPPQKIFVPLSQS"
BASE COUNT          460 a          276 c          303 g          446 t
ORIGIN      
        1 tgtgatacca ttcatcgttt tagtggttgc attctccctc ctttatttcc tgacccacaa
       61 tgaactatac gcaggtctgg gttctatttt aagcctcatt ccctattatt tggttttatt
      121 tatactaaga aactggatgc acaaaaaatt ttcgtttaaa gttcatccat tggaataaaa
      181 tgaattattt tacaacattt catgttttct ttcctgcatt ttgaggaata cttaagaaat
      241 atctgtaatt tagaaagaat ttaaagaaag tttgttaaaa taaagtaaat caggacttag
      301 gctaatatgt aaacaatcgg tgttcaggaa attttatatt gataatcagt catttactat
      361 aaagataata ttttttagta gaagacatct taaaacaatg aatcttcagg gttttatact
      421 tttgcggtga aaattcagaa atctaactaa ggtcgaaaaa tctcaaagct gtgaatccat
      481 cgattttata tgcagtaatt acgttagcag ttataggagc tggctctgga gctatacttt
      541 attgggtagc cagtaaattc aaggtagaag aggaccctcg catcgatacg gtagcagagg
      601 ccttaccgca agcaaattgt ggaggttgtg gctatgcggg ttgcaggaat tttgccgaag
      661 ctattgtaaa aacaggttct ttggataatt tgctttgccc tgtggggggc gatgaagtca
      721 tgaaaagaat tgcaagcatc ttagggttag aggctccaac catgcaaccc atgattgctg
      781 ttgtacgatg caacggcagt tttcaaaaca gtccttccaa atttcattat gatggagtta
      841 agagttgtgc ctttgcccat attactttta ccggtgaagg aggctgcccc tttggttgct
      901 taggtcaagg ggattgtgaa agagcgtgta gtttcgatgc tattcatatc aatcctaaaa
      961 ccggacttcc cgaagtgaac gataagtgca cagcctgtgg cgcatgtgtg aaagcctgcc
     1021 cgaggaatat catcgaaatg aggccaaaag ggaaaaaaga cagacggatt tttataactt
     1081 gcatgaatca ggaaaaaggt gccattgctc gtaaaaattg tgaagcagct tgcattggat
     1141 gcaacaaatg cgtcaaggaa tgccgcttcg atgccatcac tgtcagcaat aatctagcct
     1201 atatcgatcc tcataaatgt accctttgca ggaaatgtgt cgaagtttgt cctactaact
     1261 gtatactcga ggtgaatttt cccccgcgta aacgtactac tcaatccgat tcacccgcag
     1321 aaattacagc atgatataaa ttttcaatcc tctaaaatag gagatcagat gttgaagaca
     1381 ttcaaaaaag gtggtgtaca tccacacgaa aacaaatttt cggcaaatag tcccattcag
     1441 cccattgatc ccccccaaaa aattttcgtt ccattgagcc agagc
//
LOCUS       DAORPD010000507         1484 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_16856_length_1484_cov_2.369489, whole genome shotgun sequence.
ACCESSION   DAORPD010000507 DAORPD010000000
VERSION     DAORPD010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16856_length_1484_cov_2.369489"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="PK976_06275"
     CDS             complement(<1..194)
                     /locus_tag="PK976_06275"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005318430.1"
                     /note="catalyzes the formation of ornithine and
                     carbamylphosphate from citrulline in the arginine
                     catabolic pathway; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase subunit F"
                     /protein_id="HRC67528.1"
                     /translation="MAFNLRNRSFLKLLDFTSEEIKYLLGLSKELKKAKYTGTEHQRL
                     KGKNIVLLFEKDSTRTRCAF"
     gene            complement(230..1174)
                     /gene="arcC"
                     /locus_tag="PK976_06280"
     CDS             complement(230..1174)
                     /gene="arcC"
                     /locus_tag="PK976_06280"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HRC67529.1"
                     /translation="MDKLAVIAFGGNALLRGDQKGTIDEQEANVYETCTYLVPLIKQG
                     YNFVIGHGNGPQVGNVILQHQAGEKMFGIPAQPIDFCVAETQGSIGYMIEQQLRNVLI
                     EEHLERDILTIITEVIVDKDDPAFHNPTKPVGPFYSKEEADKIAAETSAKFHEDPRGR
                     GWRKVVASPMPLQINNVKSIEKLARDGQIIIAVGGGGIPVFWVHSNKLQGIDAVIDKD
                     LASSLLAVEIKADIFIILTDIPKVYINFHKPDEKALDRMSITEARRYLSDNQFSEGSM
                     APKVRACIKFVEKSGKEALITMASELGKPGAGTIIYAE"
     gene            complement(1302..>1484)
                     /locus_tag="PK976_06285"
     CDS             complement(1302..>1484)
                     /locus_tag="PK976_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67530.1"
                     /translation="HIGFYQEIFESSFSVFNIYVGFSSHSLQNYPKCRYYVFSRNKKQ
                     RGIVELIFLHRFAVDF"
BASE COUNT          438 a          319 c          260 g          467 t
ORIGIN      
        1 tcaaatgcac aacgcgttcg tgtagaatcc ttttcgaaca acaacacaat attcttccct
       61 ttcaagcgct gatgctctgt gccggtatat ttcgccttct tcagctcctt tgaaagacca
      121 agcaaatatt tgatttcttc agatgtaaaa tcgagaagtt tcaaaaaact gcgatttctt
      181 aaattgaatg ccatacgtat atctcctttt tattttgatg gttgataatt tattcagcat
      241 aaataattgt tccggcacca ggtttaccaa gttcactggc catagtaatc aaggcttctt
      301 ttcctgattt ttcgacaaac ttgatgcagg ctcttacttt tggcgccata cttccttccg
      361 aaaattgatt atcagaaagg tatcttcttg cttcggttat gctcatccta tccagagcct
      421 tttcatcagg tttatgaaaa ttgatatata ccttaggaat atcggttaaa ataataaata
      481 tatcggcttt aatttcaaca gcgagtaatg aagaagctag atctttgtcg attacggcat
      541 cgattccttg aagcttattg gaatgaaccc aaaaaaccgg aatgcctcct ccacccaccg
      601 caataataat ctggccgtca cgtgctagct tttcgatcga ttttacattg ttgatttgca
      661 gaggcatggg cgaagctaca accttacgcc agcctctccc ccgaggatct tcatgaaatt
      721 ttgcactggt ttcggctgct atcttatcgg cctcctcttt cgaataaaac ggcccaacgg
      781 gtttcgtagg gttatgaaaa gccggatcat ccttatctac aattacttcg gtaatgatgg
      841 tcaaaatatc tcgctcgagg tgctcctcga tgagaacatt gcgtaattgc tgttctatca
      901 tatatccgat agatccttga gtttcagcca cacaaaaatc aatgggctgg gctgggatac
      961 caaacatctt ttctcccgct tgatgttgaa gaataacatt ccccacttgc ggcccattgc
     1021 catgcccaat cacaaaatta tatccctgct tgatgagagg cactaaatat gtacaggttt
     1081 catatacatt ggcttcctgc tcatcaatgg ttcccttttg atctcctcgt agaagagcat
     1141 tgcctccaaa ggctattact gcaagtttat ccataaaatg actttttcgc aaaataaatt
     1201 gaacaataac tgatgatttg ctattgtact cgatgctgaa aacaaaaaaa caagggccga
     1261 aattttgtcc ttgctcaatg cttacagcta atgaatcgat tttaaaaatc gactgcaaat
     1321 ctatgaagaa aaattagttc gacaattcct ctttgctttt tatttctgga aaaaacgtaa
     1381 taacggcatt ttggataatt ttgcaaggaa tgagaagaaa atccgacata aatattaaaa
     1441 accgaaaagc tggattcgaa tatttcctga tagaaaccta tgtg
//
LOCUS       DAORPD010000508         1481 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_16907_length_1481_cov_2.039972, whole genome shotgun sequence.
ACCESSION   DAORPD010000508 DAORPD010000000
VERSION     DAORPD010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16907_length_1481_cov_2.039972"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..>1481
                     /locus_tag="PK976_06290"
     CDS             62..>1481
                     /locus_tag="PK976_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67531.1"
                     /translation="MVFSTYSGSEADNWGFTGTYDEAGNVYSGGIVFNIGYPVSLGAY
                     QQSFGGGCDIGIIKYDSTGTNRIYATYLGGLYADLPHSLIVNTSNELVIFGTTGSPNF
                     PVTPSAYDTSFNGGDAVNYASLNFPLGSDIFIAKFSEDGSQLIASTFIGGSENDGLNF
                     RSRYASMLMTGNDTLYYNYGDGARGEVITDPQDNIYIGSTTFSNDFPITAATFQPSYK
                     GKQEGVVCKFDRTLSYLNWSSYLGGSNDEGIFSIDINSYGDLYVTGGTTSSDFPVTSG
                     AYRTQFNGGSADAFISCISANGVNLKGSTFWGSLSYDQGYFVRIGPDDEVYVFGQTKA
                     SDSSMMIDALYGQPNSGQFISKFSPLLDELRWSTVVGKGDGQPNISPTAFMVDVCGRI
                     YYSGWGRIWGNSYVNGNWYLWGTPFGTHGLAVTPDAVQPITDGQDFYIAILSSEAAGL
                     DYATFFGEQHYSGCSFSGHDHVD"
BASE COUNT          401 a          288 c          335 g          457 t
ORIGIN      
        1 gataaaattt catttagatc catcattcct caactctgac aaaattgtta ttgatccccg
       61 tttggtattt tccacgtatt caggaagcga ggccgacaat tggggtttta ctggtactta
      121 cgacgaggca gggaatgtat attccggagg aattgttttc aatattggat atccagtaag
      181 tttaggagct tatcaacaaa gttttggtgg tgggtgcgac ataggcatta tcaaatacga
      241 ttctacggga acaaatcgca tctatgctac ttatttgggt ggactttatg ccgatctgcc
      301 gcatagttta atcgtcaata catccaacga actggttatt tttggtacca ctggttcgcc
      361 caactttcca gttacgccat cggcttacga tacaagtttc aacggagggg atgctgttaa
      421 ttatgcatct ttaaattttc ctttaggctc cgatattttt attgctaaat tcagtgaaga
      481 cggctcacaa ttaatagctt ctacttttat aggaggcagc gaaaacgatg gcttaaattt
      541 tcgctcacgc tatgcatcca tgcttatgac aggcaacgat actttatact ataattatgg
      601 agatggtgcc cgtggcgaag taatcaccga cccgcaagat aacatttata ttggctccac
      661 cacattttcg aacgattttc cgataactgc cgcaacattt caaccctctt ataaagggaa
      721 acaggaaggt gtggtatgta aatttgacag gacattgagc tatttgaact ggagcagtta
      781 tttaggcgga tcgaacgatg aaggtatatt ttccatcgat atcaactctt atggcgattt
      841 atacgttact ggtggcacca ccagcagcga tttccccgta acatctggag cctatcgaac
      901 ccaatttaat ggaggaagtg ccgatgcgtt tatttcgtgc atttcggcta atggagtaaa
      961 tttaaaggga tcgacctttt ggggatcgct atcttatgat cagggatatt ttgtgcgtat
     1021 cgggcccgat gatgaagtat atgtattcgg gcaaactaaa gcctctgatt cgtccatgat
     1081 gatcgatgcc ctttatggcc agccaaacag tggacaattc atatcgaaat tttcacctct
     1141 actcgatgaa ctgagatgga gtacagtagt tggaaaaggc gatggccaac ccaatatttc
     1201 tccaacggca tttatggtcg atgtatgtgg gaggatttat tattcgggat ggggaagaat
     1261 atggggtaac agttatgtaa atggtaactg gtatttatgg ggtacccctt ttggaactca
     1321 cggccttgcg gttactcccg atgctgttca acccatcacc gatggacaag atttttatat
     1381 agccatatta tcatccgaag cggcaggatt agattatgct actttttttg gagaacagca
     1441 ctattcgggt tgttcattca gtggtcatga tcatgtggat g
//
LOCUS       DAORPD010000509         1481 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_16909_length_1481_cov_1.887097, whole genome shotgun sequence.
ACCESSION   DAORPD010000509 DAORPD010000000
VERSION     DAORPD010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16909_length_1481_cov_1.887097"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(453..>1481)
                     /locus_tag="PK976_06295"
     CDS             complement(453..>1481)
                     /locus_tag="PK976_06295"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HRC67532.1"
                     /translation="CYLVPAFIFKGFLPIVISEMTGLIAFLDTLVSLYIVFVFLITID
                     AFLNAVHDLYLTTPISKNKPIKGFIQILKIIIYFIGAVVVIAILLKRNPLAILGGLGA
                     ISAILLLIFRDSILGFVAGIQLSANNMVMIGDWITMQKYDVDGTVLEITLTTVKVENF
                     DKTISFVPAYTLVSDSFRNWRGMEEAGGRRIARSLQIDMGSVKFCTPEMLEQFAQIKL
                     IRDYVLKKEEELRLFNQQFGVDGSVVVNGRRQTNLGIFRAYIREYLKSNPDVHQGMTV
                     MVRHLAPTEKGIPLQVYCFAKTTRWETYEKIQADIFDHLIAVLPYFELKVFQNPTGSD
                     FRALVGQS"
BASE COUNT          536 a          281 c          230 g          434 t
ORIGIN      
        1 tagtatgagt gcagaagtga aataaaaaca atttaaaatt ataagataga gtataattaa
       61 ataatatttt aataattaaa aaagtaatta agtaagaaat catttataat tttttagttc
      121 ttgaatgttt atttaaaatt taatttttag tttaaaataa taatcgccat ttgaattgac
      181 gaaattacat attataattg agataccgaa aacgtttgca agaacttcgc ctcgactttc
      241 tactaattta aaataattct aaatgaaaaa tactcattca aaaaattcat ttatagttta
      301 ttgtaacgat acaagtgagc aatataaata ttttattttc tttctcatcc atcattccta
      361 aataataagg ccaatagaaa aacaaaacgc aaattccgtt caaaaaaatc aatgaaattt
      421 agccagcaaa tgaaccataa aaatggacat ccttacgatt ggcccaccaa agccctaaaa
      481 tcgcttccgg ttggattctg gaataccttc aattcgaaat agggtaaaac ggcaatcaaa
      541 tgatcgaaaa tatcggcctg aatcttttca taagtttccc aacgggtagt tttggcaaaa
      601 caataaacct gcaaggggat ccctttttca gtaggagcca gatgtcttac cataactgtc
      661 attccctgat gtacatcggg attgcttttc agatattcac ggatgtaagc tctgaaaatt
      721 ccaagattgg tctgccggcg gccattaact accacagaac catcaacacc aaattgctgg
      781 ttaaatagtc taagttcttc ttccttcttc aacacataat cccttattag tttgatttgc
      841 gcaaattgtt ctaacatttc gggagtacaa aattttacac ttcccatatc aatttgaaga
      901 gaacgagcaa tacgccttcc cccggcttct tccatgcctc tccagttgcg gaatgagtca
      961 gaaaccaatg tatatgcagg tacaaacgaa atcgtcttgt caaaattctc tacttttact
     1021 gtcgttagcg taatttccaa aacagttcca tccacatcat acttttgcat ggttatccaa
     1081 tcgccaatca tgaccatatt gttggccgat agctgaattc cagcaacgaa accaagtatg
     1141 ctatccctaa aaatcaataa caaaatagcc gaaatggcac ccagcccgcc aagaattgcc
     1201 agaggatttc gcttgagcag aatggcgata accaccactg cacctataaa ataaatgatg
     1261 attttcagaa tctggataaa ccccttgatg ggtttgtttt tcgatatcgg tgtagttaaa
     1321 tataaatcgt ggacagcatt taaaaaagca tctatagtga taagaaagac aaaaacaatg
     1381 taaagagata caagagtatc aagaaaggca ataagacccg tcatttcact tatgacgata
     1441 ggaagaaatc ccttgaaaat aaaggccggt actaaatagc a
//
LOCUS       DAORPD010000510         1481 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_16911_length_1481_cov_1.769986, whole genome shotgun sequence.
ACCESSION   DAORPD010000510 DAORPD010000000
VERSION     DAORPD010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16911_length_1481_cov_1.769986"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..1084)
                     /locus_tag="PK976_06300"
     CDS             complement(2..1084)
                     /locus_tag="PK976_06300"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF015647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HRC67533.1"
                     /translation="MFYNGKNHYKGLGMMSGTSLDGVDLAACDFNFENDNWNFEILQA
                     ETIPYPSFWVDRLNRVMEASAAELASLNMDLGKYYGKIAFEFLNHHKLEVDYIASHGH
                     TVFHDAEKGFTFQAGNGVAIAAATHQPVIYDFRTADIVRGGQGAPLVPIGDELLFNSY
                     SACINLGGFANISYNKDGKRIAFDLCPANMALNFLAQKANLPFDYNGELAASGRIIQP
                     LLFELLHLEYFSKNPPKSLGKEWFLQSFLPILNKYEGEQIQDLSRTVCELIAIVIMRS
                     LFDVQPKEKGVLITGGGAHHHFLLQRIKELSTLPIAIPSPCIIDFKEALVFAFLGLLF
                     LEQKPNILSSATGAETDSIGGCLYLP"
     gene            complement(1074..>1481)
                     /locus_tag="PK976_06305"
     CDS             complement(1074..>1481)
                     /locus_tag="PK976_06305"
                     /inference="COORDINATES: protein motif:HMM:NF014874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HRC67534.1"
                     /translation="NYRPSNFRSQFIKTQHNKLVMDAYNANPSSMEAAITNFVSRFES
                     PKAAILGEMLELGLESKKEHQAIVDKIASANIQKLFFVGKNFSDIALPPTAMHFENAT
                     LLKDYLRKEPLQQYFILIKGSRGNKLETLLDVL"
BASE COUNT          465 a          305 c          293 g          418 t
ORIGIN      
        1 atcatggcaa ataaagacat ccaccaatgc tatcagtttc agctcctgtg gccgatgaca
       61 aaatatttgg cttttgctcg agaaacaata atccgagaaa ggcaaaaacc aaagcctcct
      121 tgaagtcgat aatacaaggg gaaggaatgg caataggtaa agtagataat tctttaattc
      181 tctgtagaag gaaatgatga tgcgctccgc ctccggtaat taaaacgcct ttttcttttg
      241 gttggacatc aaataatgaa cgcataatca caatagcaat caactcacaa accgtacgcg
      301 aaagatcttg aatctgctcg ccttcgtact tatttaaaat gggaagaaaa gattgcaaga
      361 accactcctt tccaagtgat ttagggggat ttttcgagaa atactcgaga tgcagaagtt
      421 cgaagagcaa aggctgaatg atgcgaccag aggcggccaa ttctccatta tagtcgaagg
      481 gcagattcgc tttttgagca agaaagttca aagccatatt ggccggacat aaatcgaaag
      541 ctatgcgttt cccatcttta ttataagaaa tattggcaaa ccctcccaga ttgatgcaag
      601 cggaatagga attaaaaagc aattcatcgc caatgggtac cagaggtgca ccttgccctc
      661 ctctcacaat gtcggctgtt ctgaaatcat aaattaccgg ttgatgagtg gccgctgcaa
      721 tggctactcc attaccggcc tgaaaagtaa agcctttttc tgcatcatga aagacagtat
      781 gaccatgaga ggctatataa tcaacttcta atttatgatg attcaaaaat tcaaaggcta
      841 tttttccata atactttccc agatccatat tcagactggc aagttcggca gcagaggctt
      901 ccattacccg attcaggcga tcgacccaga aagaaggata tggtatggtt tcggcctgta
      961 aaatctcgaa attccagttg tcattctcaa aattaaaatc acaggctgcc aggtcaacgc
     1021 catccaagga tgtacccgac atcattccca gacctttgta atgatttttg ccgttataaa
     1081 acatcgagta aggtttctaa tttatttcct ctacttcctt taataagtat aaaatattgt
     1141 tgaaggggtt cttttcttaa ataatctttc aagagtgttg cattttcgaa atgcattgct
     1201 gtgggtggca aggctatatc cgagaaattc tttcccacaa aaaataattt ctgaatgtta
     1261 gccgaagcaa tcttatctac tattgcctga tgttctttct ttgattccaa tcccagttca
     1321 agcatttcac ccaaaattgc tgccttaggc gactcgaagc gagagacgaa attagtgatg
     1381 gcagcctcca tactcgaagg attggcatta taggcatcca tcactaactt attgtgttga
     1441 gttttgatga actgagaacg gaaattacta ggacgataat t
//
LOCUS       DAORPD010000511         1479 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_16947_length_1479_cov_3.196629, whole genome shotgun sequence.
ACCESSION   DAORPD010000511 DAORPD010000000
VERSION     DAORPD010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16947_length_1479_cov_3.196629"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1136)
                     /locus_tag="PK976_06310"
     CDS             complement(<1..1136)
                     /locus_tag="PK976_06310"
                     /inference="COORDINATES: protein motif:HMM:NF013614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HRC67535.1"
                     /translation="MEIVNQSLLDTSYWKEIFQQHRDAITHYDTPLLTKMRNEALENF
                     LSSGIPDLNHERWRNTDIRKFFRNDWNFLFQMPDNHGKIEDFFLCEVQGFDTLLITLF
                     NGWYTYKNSPLRILTDGTIIGGLRAALVEAPEIIERYLGRFADFRQNAFTSLCTAFAS
                     EGFFIYVPDGVQLQKPIQMVNVVNYSQPLFMQTRNLIIAGKGSSIQLLHCDDSNNNEP
                     AFTNSLTEIYVGEGASVDYYKLQNINDHSVIEHNAYIHQEKQSVCNTYSINLHGGLIR
                     NETYNHLAGHHAEGNHFGLYLMDKNQQADNLIFVDITASDCVSYELFKGVLDDYAHGV
                     FNGHILVHNDGQRTQAYQSNRNILLTDKALVNTKPFLEIYADDV"
     gene            complement(1155..>1479)
                     /gene="sufC"
                     /locus_tag="PK976_06315"
     CDS             complement(1155..>1479)
                     /gene="sufC"
                     /locus_tag="PK976_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005927288.1"
                     /note="part of SUF system involved in inserting
                     iron-sulfur clusters into proteins; in Escherichia coli
                     this protein forms a complex with SufBD; the SufBCD
                     complex stimulates the cysteine desulfurase SufS in
                     conjunction with SufE; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HRC67536.1"
                     /translation="GFSGGEKKKNEIFQMAMLEPTLAILDETDSGLDIDALRIVANGV
                     NKLRRPDNAFIVITHYQRLLNYVIPDFVHVLYDGKIVKSGKKELALELEEKGYEWIKK
                     EVASL"
BASE COUNT          424 a          296 c          312 g          447 t
ORIGIN      
        1 tttacatcat ctgcataaat ctcgagaaaa ggcttggtat tcaccaaagc cttatcggtt
       61 agaagaatat ttcggttact ttggtaagct tgtgtgcgtt ggccgtcgtt gtgaacgagg
      121 atatggccat tgaaaactcc atgtgcataa tcgtcgagca cacccttaaa taactcataa
      181 ctcacgcaat cggaagcggt aatatctaca aagatcagat tatcggcttg ctgattctta
      241 tccatgagat aaagaccgaa atgatttccc tcggcatgat ggccggccaa atgattgtaa
      301 gtttcattac ggataaggcc tccatgtaaa ttgatgctgt aagtattgca aacgctttgt
      361 ttttcctgat ggatataagc gttgtgttcg attacgctat gatcgttgat attttgtaat
      421 ttgtaataat caacactcgc cccttctcca acgtaaattt cggtaagaga gttggtaaag
      481 gcaggttcgt tattgttgga atcgtcacaa tgaagcaact gaatactgct tcccttcccg
      541 gcaatgatca gattacgagt ttgcataaac aatggctggg aataattcac tacattgacc
      601 atctgaatag gcttttgcaa ttgcacacca tcgggcacat aaatgaagaa tccttctgaa
      661 gcaaaggcgg tgcaaagaga agtgaaggcg ttctgtcgaa aatcggcaaa gcggccgaga
      721 tatctttcga tgatttcagg agcttccacc agggctgccc tcaggccgcc aataatggtc
      781 ccatcggtaa gaatccgtaa gggagaattc ttataagtgt accagccatt gaaaagggta
      841 ataagtaagg tatcgaatcc ttgcacttca cacaggaaga aatcttcgat cttaccatga
      901 ttatcgggca tttgaaataa aaaattccaa tcattcctga aaaattttct aatatcggtg
      961 tttcgccagc gttcgtggtt tagatcggga atacctgacg ataaaaaatt ctcgagagcc
     1021 tcattgcgca tttttgtgag cagaggagta tcgtaatgcg taattgcatc cctgtgttgt
     1081 tgaaagattt ctttccaata tgaagtatcg agtaacgatt gatttacgat ttccatatca
     1141 tcaatgcttg aaaattataa ggaagcaact tcttttttta tccattcgta gcctttttct
     1201 tcgagttcga gggcgagttc cttttttcct gattttacga tttttccatc gtaaagcaca
     1261 tggacaaaat cgggaattac ataattgagg agtcgctggt agtgtgtaat cacgataaag
     1321 gcattatcag ggcgtcgcaa tttattcacg ccattggcca ctatacgcaa agcatcgata
     1381 tcgaggcctg agtcggtttc atctaaaata gctaatgtag gttcgagcat ggccatttga
     1441 aaaatctcgt tctttttttt ctctcccccg ctaaaccct
//
LOCUS       DAORPD010000512         1479 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_16948_length_1479_cov_3.100421, whole genome shotgun sequence.
ACCESSION   DAORPD010000512 DAORPD010000000
VERSION     DAORPD010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16948_length_1479_cov_3.100421"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(751..>1479)
                     /locus_tag="PK976_06320"
     CDS             complement(751..>1479)
                     /locus_tag="PK976_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HRC67537.1"
                     /translation="GSGLPGKLADCSERDPVMAELFLVEGDSAGGTAKQGRDRRFQAI
                     LPLRGKILNVEKAMQHKIFDSEEIKNIFTALGVSIGTDEDSKALNLEKLRYHKIIIMT
                     DADVDGSHIATLILTFFFRYMKELIENGYVYIATPPLYLIKKGNIERYCWTEEERINI
                     VAELSKDGSDKGIHIQRYKGLGEMNAEQLWSTTMDPERRILRQVKIENGTEADRIFSM
                     LMGDDVAPRREFIERNARYANIDA"
BASE COUNT          423 a          292 c          291 g          473 t
ORIGIN      
        1 gtggtcaggc aacggttttc tatctcccac gtcctacacc taaacccata aaacatgatt
       61 gattcgatta aaaatattat ttaacataga acaagttctc aacttcagaa cttttgattg
      121 ggttttaatc aggttgtcgt ggttttgatg agagcgtttc agcattacct gctctaaata
      181 attacttctg tcatcctctg gaggtatact tctaccatct gcaatcatta attttttaaa
      241 tttcttttcc tatggttaaa atcttaaagg ttcttagccc gttagcgaaa aaacattgca
      301 gcagtggcac agaggccaca aaggaacaaa ggattgaaat gatttttctt cgagtccttg
      361 gcgtcttggc gagaaaaaca ttacacgcag gggtgcaaaa gccacaaagg aaattgaaac
      421 tcttctttta tcgtttctta ggccactgaa atttaaacaa aacccaatag atttttaagg
      481 tggtactttg tcacgtcagt tatgacgaga ttgatatgca attataggat caagagttct
      541 gaaaatattg ataagcaatt aaaaaatctc gagaccctat actattttct aatatattcg
      601 gtatttatca attaatttat ttatcgggca aaatattaaa gttgagtttt ttacagcaat
      661 gattttgaat tgataaaaca attcggagtt atgaaggcag ggatttgtca atgcaataga
      721 ggcattaata aaatgcgttt tcaatacgcc tcatgcatct atgttagcat atctggcgtt
      781 acgttcaata aattctcttc tgggtgccac atcatcaccc atcaacatag agaaaatgcg
      841 gtcggcttca gttccatttt cgattttaac ctggcgtaaa atacgccttt cggggtccat
      901 agtggtgctc cagagttgct cggcattcat ttcacccaaa cccttataac gctgtatatg
      961 aattccctta tccgacccat ccttcgagag ttcggctaca atatttattc gttcttcctc
     1021 ggtccagcag tacctttcta tatttccctt tttgatgaga taaagaggag gagtagcgat
     1081 gtaaacgtaa ccgttttcga tgagttcttt catataacgg aagaagaagg taaggataag
     1141 tgtagcgata tgactaccat ccacatcggc atcggtcatg atgatgattt tgtggtagcg
     1201 aagtttttcg agattcaggg ctttactgtc ttcatcggtt ccaatagata ctcccagagc
     1261 ggtaaaaata ttcttaattt cttcgctgtc gaatatttta tgttgcatgg ctttttcgac
     1321 gttaaggatt ttacctctaa gaggcagtat agcttggaag cggcggtcgc ggccttgttt
     1381 ggcggttccg ccggccgaat caccctcaac gaggaagagt tcggccataa cagggtctct
     1441 ttcactgcaa tcggctaatt ttccaggtaa acccgaacc
//
LOCUS       DAORPD010000513         1479 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_16953_length_1479_cov_1.960674, whole genome shotgun sequence.
ACCESSION   DAORPD010000513 DAORPD010000000
VERSION     DAORPD010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16953_length_1479_cov_1.960674"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            357..869
                     /locus_tag="PK976_06325"
     CDS             357..869
                     /locus_tag="PK976_06325"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRC67538.1"
                     /translation="MECPVCHRQGIDSLLQKCPECGADLTPLGRIQKLRQRVKRNYTL
                     MLSLIVILVIANLIGIFQYHSSTSLTKTKLLSDSVTQLIQVNQELSDSLNILKDSLQK
                     LFVAQKEYSKTEESTTLEHIVVKGESLWKIAEKYLGDGSLYSKIARDNFLSDPSQIAE
                     GQKIIIHKNP"
     gene            882..1403
                     /locus_tag="PK976_06330"
     CDS             882..1403
                     /locus_tag="PK976_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67539.1"
                     /translation="MKTLHVIDEILIETTNYLQEIWDLTTQNSELTNNLAQCQENLKS
                     TLETLKKREESLREKQAYIENLEKIKADLESQLLQSQNLCSDLRSQLLEKEASLNQIR
                     KENKNLSNEIAVLQNKIEQAENRIQELNSKNLELESLLHETQENLKNARFEIENLQQS
                     LPPEQLNNDETNE"
BASE COUNT          568 a          284 c          230 g          397 t
ORIGIN      
        1 aatattgaac ttgacatagg caataactca atatgcccga aatgcaaaaa tatagtggta
       61 tggcgtaaag gttatcatac ggctgcaaaa ggtttggatg aatcaggaaa ttgcaggttc
      121 tgcaaaacat ctctcccttt cgtagtttaa tgatgaaaaa tgcatcttcc cactaacggc
      181 aatcattctt ctatctaaat cttccaacct ccttaacaat cttattttta gaaaagcact
      241 cattaaaaaa agaatttttc ccgagtagta atttcttaga tatattgata aataatctat
      301 atctttatag ccgaatttaa ttttatcttc ctgatgttca actaaaaaaa tttaccatgg
      361 aatgcccggt ttgtcatcgc caagggatag actccctttt gcaaaaatgc cccgaatgcg
      421 gagctgacct tacccctctg ggtcgtattc aaaagctcag gcaaagggta aaaagaaact
      481 acaccttgat gctgtcgcta attgtaatat tagtaatagc caacctcatt ggaatttttc
      541 aatatcattc atcaacctcc ttaaccaaaa caaaattact gagcgattct gttacccaat
      601 taatacaggt aaaccaagaa ctaagtgatt cgttaaacat cctcaaggat tcgttacaaa
      661 aactttttgt tgcccaaaaa gaatactcta aaactgaaga atccactacc ctcgaacaca
      721 tcgtagtcaa gggcgaaagt ctatggaaga ttgcagagaa atatttagga gatggcagtc
      781 tttattcaaa aatagcccgc gataatttct tgagtgaccc ttcacaaata gcagaagggc
      841 aaaaaatcat cattcataaa aacccgtaaa atctaataac catgaaaact ctccatgtaa
      901 tagatgagat actgatagaa actacaaatt acttgcagga aatatgggat ttaaccacgc
      961 agaatagtga acttacaaat aatttggctc aatgtcagga aaacctgaaa tccacactcg
     1021 agaccttaaa aaagagagaa gaaagcctgc gagaaaaaca agcttatatt gaaaatctcg
     1081 aaaaaattaa agctgacttg gaaagccaac tcttgcaatc gcaaaacctc tgttccgatt
     1141 tgcgatcaca acttcttgaa aaggaagcat cattgaatca aataagaaaa gagaacaaaa
     1201 atctatccaa tgaaatcgca gttttacaaa acaagataga gcaagccgaa aacaggattc
     1261 aagaattaaa ctcgaaaaat ttagagctcg aatcattatt acacgaaact caagaaaatc
     1321 tcaaaaatgc tcggtttgaa atagaaaatc tccagcaatc tcttccccca gagcaattaa
     1381 ataatgacga aacaaatgaa taatttattc ttgttattaa ttgaggtttc atagaaaaca
     1441 atcgatagaa tcattattaa aaaagccggt tattctatc
//
LOCUS       DAORPD010000514         1478 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_16974_length_1478_cov_1.985242, whole genome shotgun sequence.
ACCESSION   DAORPD010000514 DAORPD010000000
VERSION     DAORPD010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16974_length_1478_cov_1.985242"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..905)
                     /locus_tag="PK976_06335"
     CDS             complement(69..905)
                     /locus_tag="PK976_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67540.1"
                     /translation="MSFSCNFTYKFISPFMNMTIAEKNLIIDEIFDMIEVLKEQLNSF
                     KTDEERIPQIELDLCMENVRRLYEKLKLLDSLNMGTRPKSQPAPSVSKQPASIRIRFE
                     GVEKNIPEKREPPSPTETSISHPSSTNFKEPEPFAKPQPSEEPAKKQHSKPKTVPFTP
                     DLFSQEIPIIADKLAEEKPTLIDKLAVHTDDKSIVDNLTNRPLADLRAAIGINEKFLF
                     VNELFGGSLQEYNQAVQALNQAGNLDNALEIFNSYQLRYRWNPKLPGVNKLKELIQRR
                     YL"
     gene            927..>1478
                     /locus_tag="PK976_06340"
     CDS             927..>1478
                     /locus_tag="PK976_06340"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HRC67541.1"
                     /translation="MFLELPLVKKQPSGWIEVITGSMFSGKTEELIRRLRRAHFAKQN
                     VEIYKPDIDTRYHEQDVVSHDANAIPCTPVVSSQQILLYVGDVDVIGIDEAQFFDMEI
                     VNVCQQLADEGKRIIVAGLDMDYKGQPFGPLPALMACAEFVTKVHAICMDCGNLAHYS
                     FRTVPDKKLVLLGETESYTPLCRN"
BASE COUNT          400 a          280 c          335 g          463 t
ORIGIN      
        1 tcaaaacaaa tcgaaccaga ggaggtgaat gctatctgga caggattcaa aatgaatgct
       61 caatagtttt ataaatatct tcgctgaatg agttctttta gtttattgac tcccggaagt
      121 tttggattcc aacggtaacg taattgatac gaattaaata tttcgagtgc attatcgaga
      181 tttcctgcct gattcaaagc ctgaacggct tgattgtatt cctgcaaact ccctccgaaa
      241 agctcgttta caaacaagaa tttctcattg ataccgatag cagctcgaag atcagccaaa
      301 ggccggtttg tcagattgtc tactatactc ttgtcgtcgg tatgtacggc aagcttgtcg
      361 atcaatgtcg gtttttcttc ggcaagttta tcggcaataa tgggtatttc ctgactgaac
      421 agatcaggag taaaaggcac ggtttttggc ttggaatgct gttttttggc gggttcttcg
      481 gaaggttgag gtttggcgaa aggttcgggt tctttaaaat ttgtagatga gggatgcgat
      541 atactggttt cagtaggaga aggtggttcc ctcttctcag gaatattttt ttctactcct
      601 tcgaaccgga tacgaatgga agccggttgc ttcgatacgg agggtgcggg ttgtgatttt
      661 ggccttgtgc ccatattgag gctatcgagc aattttaatt tttcataaag ccggcgtacg
      721 ttttccatgc aaagatcgag ctcgatttga ggaattcttt cttcatccgt tttgaatgaa
      781 ttcaattgtt ccttaagcac ttctatcatg tcgaaaattt catcgataat tagattcttt
      841 tctgctatcg tcatattcat gaaaggtgaa ataaatttgt aggtaaaatt acacgaaaaa
      901 ctcaagaaat tttattttta atcgaaatgt tcttagaatt acctcttgta aaaaaacaac
      961 cttcaggatg gatcgaagtg atcactggtt ccatgttttc tggaaagact gaagagttga
     1021 tccgtcgtct aagacgggcg cattttgcca aacaaaatgt cgagatatat aaaccagata
     1081 tcgatactcg ttatcatgag caggatgtcg tatctcacga tgctaatgct attccatgca
     1141 caccggttgt ttcgtctcag caaattctgc tgtatgtagg cgatgtggat gtaattggca
     1201 tagacgaagc ccagtttttc gatatggaaa ttgtaaatgt atgccaacag cttgccgacg
     1261 aaggcaagag gattattgtg gcaggtttgg atatggacta taaggggcaa ccatttggtc
     1321 cgttgcctgc cctgatggcc tgcgccgaat ttgtaaccaa ggtgcatgcc atttgcatgg
     1381 attgcggaaa tttggcccat tattccttca gaaccgttcc tgacaaaaaa ctcgtcctac
     1441 tgggtgaaac tgaaagctac actcccctgt gccgcaac
//
LOCUS       DAORPD010000515         1478 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_16975_length_1478_cov_1.825018, whole genome shotgun sequence.
ACCESSION   DAORPD010000515 DAORPD010000000
VERSION     DAORPD010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16975_length_1478_cov_1.825018"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /gene="argS"
                     /locus_tag="PK976_06345"
     CDS             complement(<1..794)
                     /gene="argS"
                     /locus_tag="PK976_06345"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HRC67542.1"
                     /translation="MFAQLLAQQTGYAIEALYHHIVQPNQVPVNKTKKEFTGDFTIIT
                     FPFAKISKKSPELTAGEIGEWLKNALPEIESFNVIKGYLNIILKDDFWMEFLDKNYRN
                     HSFGLNPQKQGQAPVLIEFSSPNTNKPLHLGHIRNNLLGHSVAEILEATGLPVVRVNL
                     VNDRGIHVCKSMLAWKKWSNGETPESSGLKGDHLVGNYYVLFEKKYKEEVNQLKANGL
                     DEETALRTAPLMLEAQEMLRKWEAGDAETHSLWEMMNSWVYEGFNET"
     gene            complement(834..>1478)
                     /locus_tag="PK976_06350"
     CDS             complement(834..>1478)
                     /locus_tag="PK976_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67543.1"
                     /translation="LNLLHLKHEIAVLSSEVIELFRFVISSVVPGDTSNLIQQNEDAE
                     RLYQKIQFHHTEILNYIQTISFDEMSADGQVRYRTMLLTLENFQVIVKVNQKLLQTIQ
                     EKSKEKIWFSPKQHRDLMGLLDEVDSAFQLLVNNLNGEYHKVNIAESQLIEEKINKMR
                     DKIKEKLPRKIEQGKINPLSVDYYKSLVDAAENLGDLMMEINRKLAECGKKTMP"
BASE COUNT          395 a          289 c          292 g          502 t
ORIGIN      
        1 gtttcattga agccctcata cacccaagag ttcatcattt cccaaagtga atgcgtttcg
       61 gcatctcccg cttcccattt tcttagcatt tcctgtgctt cgagcattaa tggagccgtg
      121 cgaagtgccg tttcttcgtc caaaccgttt gccttcagct gattaacttc ttctttatat
      181 tttttctcga aaagaacata ataattacct accaggtgat cgcctttaag gccactggat
      241 tcaggagttt caccattact ccattttttc catgccagca tggatttgca tacgtgaatg
      301 ccgcgatcgt ttaccaggtt tactcttact acgggcaaac ccgtagcttc gaggatttcg
      361 gcaaccgaat gccccaaaag attgttccga atatgaccaa gatgaagagg tttattggta
      421 ttgggcgatg agaattcgat caatactggt gcctgccctt gtttttgagg atttaaacca
      481 aaactgtgat ttcgataatt cttatcgaga aattccatcc agaaatcatc tttcagaata
      541 atattgagat aaccttttat tacgttgaaa ctttcgattt cgggaagagc atttttaagc
      601 cattcaccga tttcaccagc agtaagttcg ggagattttt tcgagatttt tgcaaaaggg
      661 aaagttataa tagtaaaatc tcctgtgaat tctttttttg ttttattcac ggggacttgg
      721 ttgggttgaa cgatgtggtg atatagggct tcgatagcat agcccgtttg ttgagcaaga
      781 agttgagcaa acatgtataa aagcttggag cgacaaaatt atgaaaatag gtcttatggc
      841 attgttttct tcccacattc ggccaacttc cgattgatct ccatcataag gtcccctaag
      901 ttttcggccg catcgaccag agatttataa taatcgaccg agagaggatt aatctttccc
      961 tgttcaattt ttcttgggag tttttccttg attttatctc tcattttatt gattttttct
     1021 tcaataagtt gagattcggc aatatttacc ttatgatatt ctccattgag gttattgaca
     1081 agcaattgga aggccgaatc tacttcatct aacaagccca tgaggtctct gtgttgtttg
     1141 ggtgagaacc atatcttttc tttcgatttt tcctgtatcg tttgcagcaa tttttggttg
     1201 acttttacaa ttacctgaaa attttccagt gtaaggagca tggttcggta acgcacctgt
     1261 ccatcagcac tcatttcatc gaaagaaatg gtttggatgt aatttaaaat ttcagtgtgg
     1321 tggaattgaa ttttctgata aagacgctca gcatcttcat tttgctgaat cagattcgaa
     1381 gtatctccgg ggacgaccga agagattacg aagcgaaaaa gctctattac ttcagaactt
     1441 aagacagcta tttcatgctt taagtgtaaa agattgag
//
LOCUS       DAORPD010000516         1474 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_17054_length_1474_cov_2.171952, whole genome shotgun sequence.
ACCESSION   DAORPD010000516 DAORPD010000000
VERSION     DAORPD010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17054_length_1474_cov_2.171952"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(171..746)
                     /locus_tag="PK976_06355"
     CDS             complement(171..746)
                     /locus_tag="PK976_06355"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007218055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HRC67544.1"
                     /translation="MKHELPPLPWAKNALEPYISQQTIEFHYGKHHQTYVNNLNNLIV
                     GTPFENSNLEDIILRAEGGIFNNGAQVWNHTFYWNCLMPNGRSEPMGRLGEAIKRDFG
                     SFEAFKENFTSAAVSLFGSGWAWLVKNPHGHLEIIQESNAGNPLRWGKTPLLTCDVWE
                     HAYYLDKQNRRADYVADFWKIIHWNAVEERL"
     gene            complement(834..>1474)
                     /locus_tag="PK976_06360"
     CDS             complement(834..>1474)
                     /locus_tag="PK976_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HRC67545.1"
                     /translation="LSDIAKRAIAGYLDLASSLTAFGNQNPTSYFRLVPHQEAPTNIC
                     WGDRNRSVLVRVPLGWLGKANMTIDANPLEPSGEYDFSQKQTVEFRSPDGSANVYYLL
                     AGLVVAARHGLEMPDALDYAERTYVDVNIFEEAHKDKLETLRHLPVSCWESAEQLLVQ
                     SDIYTRFGVFSPSFIQGLARQLKLYNDKNLRQEIENNSEAILKLVEKYFHCG"
BASE COUNT          417 a          325 c          287 g          445 t
ORIGIN      
        1 atatctttgc ccatggttgt taatttaggt ttactacaca aatttacaac tggggatgaa
       61 acttcggttt tgtccctcct tcattttatt caattttttt gtcggtcagt agtgaaaata
      121 aatctacatt tcagtcgaaa ccataaaagt taaaccagag tatacaggtc tcacaacctt
      181 tcttcgacgg cattccaatg gatgattttc cagaaatcgg caacataatc ggcacggcga
      241 ttctgtttgt cgagataata ggcgtgttcc catacatcac acgtcaaaag aggagtttta
      301 ccccatcgca gcggatttcc tgcattgctt tcctgaataa tctccagatg tccatgcggg
      361 tttttcacca accaagccca acccgaaccg aaaagtgaaa cagccgcact agtgaaattt
      421 tccttaaagg cttcgaatga accaaaatct cttttgatgg cttcgccaag cctccccatg
      481 ggttcgcttc taccattcgg catcaaacag ttccaataaa acgtatgatt ccatacctga
      541 gcaccattgt taaaaattcc tccttcagct cttagaatga tatcttcgag gttggaattt
      601 tcgaaaggtg ttcccacaat gagattattg aggttattta catacgtttg atggtgtttc
      661 ccatagtgaa actcaatggt ttgctgactg atatatggct ccagggcatt cttggcccat
      721 ggcagcggtg gtaattcatg tttcatggct catccttttt ttgatgatta gttagataac
      781 taattgtaaa ttttacttcc aaagatacat aaacttcttt agatggttat caatcatccg
      841 caatgaaaat atttttcgac aagtttcaag atggcttctg aattattttc gatttcctgc
      901 ctcaaatttt tatcattata taactttaat tgtcgggcaa gaccttggat aaaagaaggt
      961 gaaaatactc caaaccgtgt atagatatcg gattgtacaa gaagctgttc ggccgattcc
     1021 cagcacgaaa ccggaagatg cctgagtgtt tcgagtttat ccttatgggc ttcttcgaag
     1081 atattgacat ctacataagt acgttcggca taatccaaag catcgggcat ttcaaggcca
     1141 tgtcgggctg ctaccaccaa ccctgctaac agataatata cattggccga accatcgggg
     1201 cttcggaatt caacagtttg cttttgtgag aaatcatatt ctcccgaggg ttctaaggga
     1261 tttgcatcta tggtcatatt ggctttccct aaccatccca aaggcacgcg caccagaact
     1321 gagcggtttc tatcgcccca acaaatattg gtaggagcct cctggtgagg taccaaccga
     1381 aaataagaag tgggattctg attgccaaaa gccgtgagtg aagaagccag atctaaatac
     1441 cctgcaatgg cacgctttgc aatatcgctc aatt
//
LOCUS       DAORPD010000517         1473 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_17071_length_1473_cov_2.717913, whole genome shotgun sequence.
ACCESSION   DAORPD010000517 DAORPD010000000
VERSION     DAORPD010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1473
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17071_length_1473_cov_2.717913"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(382..>1082)
                     /locus_tag="PK976_06365"
     CDS             complement(382..>1082)
                     /locus_tag="PK976_06365"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HRC67546.1"
                     /translation="SSLVLLDDNFASIVEAIRMGRRISDNLQKALAYIFAIHVPIAGL
                     SLIPVLFANLPLILWPVHIVFMELIIDPACSIIFEAEKEEKNVMTRPPRPLDEPFFDT
                     KKILMSCAQGIGLLLMSLLVYFIGLRMGYSENAVRTFTFVTLIVSNVSVILCNRSWSS
                     SIIKIISTPNPTVKWIVGGAIFFLLLVLKIPALQDLFLFEEIGWFEIFICALAGISSI
                     IWFEIYKQLKKEKI"
     assembly_gap    1083..1092
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1093..>1473)
                     /locus_tag="PK976_06370"
     CDS             complement(<1093..>1473)
                     /locus_tag="PK976_06370"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRC67547.1"
                     /translation="GLMDPIRENIPQAIAQCYKAGIRIIMITGDYPVTAVNIGKEIGI
                     KDPELYLTGQDIEELTEEELSEKIRKVNIFARVVPEQKLKIVNALKRNQEIVAMTGDG
                     VNDAPALKAANIGIAMGEKGTDVAR"
BASE COUNT          481 a          303 c          253 g          426 t
ORIGIN      
        1 tttttaatgc gaaagtctta tctcttccgc tatgttgtac tttgttctaa atatttaata
       61 aatgtctatt ataagcccct tcagaaaaaa actttaggaa atttatagag aaaataaaag
      121 aaaatatctt ataagtaatc ctgttggtca aaaaaacgaa acattaacct cggctcatga
      181 acccagaagt cgaagatgaa tataaaaaac cataacgatt gacataaatg ggtaaaatca
      241 actaacttta gggatagttt ttaaaaaccc atccttgcaa ttttcaatcc atttcaatta
      301 aaattgtcga tgaacaaaag cacgataagc tcgcttcaac tttgaggagc gagatcaatc
      361 gataccttcg ttttctttgc tttatatttt ctccttcttc aattgtttat agatttcgaa
      421 ccaaataatg ctcgaaattc cggccaaagc gcaaataaaa atttcgaacc agccaatttc
      481 ctcgaagagg aataaatctt gtaaagcagg aattttcaat acaagaagta agaaaaagat
      541 ggcaccacct acgatccact ttacggttgg attgggtgtt gaaattattt tgattatgga
      601 ggaagaccac gaacgattgc aaagaataac agaaacattc gaaacgatga gtgttacaaa
      661 ggtgaaggtt cttacagcat tttccgaata ccccatcctt aggcctataa aatacaccag
      721 caggctcata aggagcaatc caataccttg agcgcaactc atcaatattt tttttgtatc
      781 gaaaaagggt tcatccaatg gtcggggagg acgcgtcatt acattttttt cctctttttc
      841 ggcttcaaaa atgatggaac aggcagggtc gatgatcaat tccataaaaa caatatgaac
      901 aggccaaaga ataagaggca aattggcaaa tagcactggg ataagcgaca aacccgcaat
      961 gggtacatga atggcgaaaa tataggcaag cgctttttgc agattatcgg aaattctccg
     1021 tcccatcctt atggcttcca caatagaggc aaagttatcg tcgagcaaaa ccaaagaaga
     1081 ggnnnnnnnn nnacgagcca catctgtacc tttttcaccc attgcaatcc caatatttgc
     1141 ggctttcaaa gctggtgcat catttacccc atcacccgtc atggccacta tctcctgatt
     1201 cctcttcaga gcatttacaa tctttaattt ttgttcgggc acaacacgcg caaatatgtt
     1261 aaccttccta attttttcac tgagttcttc ttctgtcagc tcttcaatat cctggccagt
     1321 cagataaagt tcaggatcct ttattccaat ttcctttcct atattgacag ccgtaacagg
     1381 atagtctcca gtaatcatga tgattcttat tccagccttg taacattggg ctatggcttg
     1441 aggaatattc tcacgaatag ggtccatcaa acc
//
LOCUS       DAORPD010000518         1468 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_17181_length_1468_cov_2.558386, whole genome shotgun sequence.
ACCESSION   DAORPD010000518 DAORPD010000000
VERSION     DAORPD010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17181_length_1468_cov_2.558386"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..784)
                     /gene="tal"
                     /locus_tag="PK976_06375"
                     /pseudo
     CDS             complement(1..784)
                     /gene="tal"
                     /locus_tag="PK976_06375"
                     /inference="COORDINATES: protein motif:HMM:NF013116.2"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
     gene            905..>1468
                     /locus_tag="PK976_06380"
     CDS             905..>1468
                     /locus_tag="PK976_06380"
                     /inference="COORDINATES: protein motif:HMM:NF039484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67548.1"
                     /translation="MQLKKWHLALLALLAGSLLALSWPSRGFPGFLFVAWVPLFFMEQ
                     YFLDCKDKNSSWSLFGWAFLAFLIWNTLTTWWIWNSTKIGSIAAVLLNSFFMAVVFDL
                     YHITRRKLWQGQQFGWLILFYWPAWEYLHLNWDFSWPWLSLGNGFASYIKWIQWYEYT
                     GILGGTFWVLVSNLLIFILLKNIFTHTI"
BASE COUNT          401 a          304 c          290 g          473 t
ORIGIN      
        1 gcctgcaaac aacctcccgc tccgtatttt cgagttcata tagtaatgca ggcgatatgg
       61 tcaaaagatc acaaccagct agggcaatta cctgatctat atttcgaaaa ctggctccca
      121 tgatttcggt aggataccca aatttcatat aataatggaa gatgtcacaa acacttttta
      181 ctccaggatc ctcttcgatt ggaacatacg taagcccttt ctcttttttg taccagtcga
      241 ggatacggcc gacaaacgga gaaataaggg tcacatgagc ttcagcgcat gccactgcct
      301 gaattttcga gaaaagtaag gtgagattgc aatgaatgcc atccttctca agggcttcgg
      361 cagctcttat cccttcccat gtactcgcaa tcttgataag aatacgtttt ctattcattc
      421 ccgcttcttc atatagatca atcaagtgcc ttgccttgga aatgcttctt tgtgtatcaa
      481 aagataatcg ggcatcaact tcggtactta ctcggccggg tacaattttt aaaatctcca
      541 gtcaaaacaa tagggctatt tcatccagac aaatactcaa ttgatgtttt ggatcaaacg
      601 actttcgttt ggctatagaa acagcttttt cgaccagata acggtacttt tcctgacagg
      661 ctgccacata gatcaatgaa ggatttgttg ttgcatctac agggcgataa gccaaaatag
      721 attcaaaatc acctatatcg gctactactt tgctgtattg tcgaagttgc tcaaggctgt
      781 tcattgcact tttaaccaaa attgagttat aattcgttgc aaaaataatg aacctctttc
      841 gattacttcg aatcattcct atcagtatgg aaaaccccat atctttgaac ccaaaaatag
      901 aaggatgcaa ctgaaaaaat ggcacttggc tctgctggct ctgctggcgg gtagtcttct
      961 tgcactttca tggccatcga ggggattccc gggatttctg tttgtggcat gggttccttt
     1021 attttttatg gaacaatatt ttcttgattg taaagataaa aatagcagct ggagcttatt
     1081 tggctgggca tttttggcct ttcttatttg gaataccctc actacatggt ggatttggaa
     1141 ttcaaccaaa ataggaagca ttgcagcagt tctactcaac agctttttta tggccgttgt
     1201 attcgatctt tatcatatta cacgtcggaa attatggcaa gggcaacagt ttggctggct
     1261 catcctgttt tactggcctg catgggaata ccttcactta aattgggatt tttcgtggcc
     1321 atggcttagt cttggtaatg gttttgcttc ttatataaaa tggatacaat ggtatgaata
     1381 tacgggtata ttggggggaa ccttctgggt cctggtctcc aatttattga tttttattct
     1441 gctgaaaaat atttttaccc acactatc
//
LOCUS       DAORPD010000519         1468 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_17189_length_1468_cov_1.773531, whole genome shotgun sequence.
ACCESSION   DAORPD010000519 DAORPD010000000
VERSION     DAORPD010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17189_length_1468_cov_1.773531"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="PK976_06385"
     CDS             <1..712
                     /locus_tag="PK976_06385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HRC67549.1"
                     /translation="LRCLWCHNPESQLNKPEPFLRMDKLEGKFFEHEEQIGYEIDLDE
                     LLEILFRDRAFMEESGGGVTLSGGEPLWQWKFSAALAQKLSESGIHVAVDTCGYVQLR
                     VFEAILPYTHLFLYDLKGYDNHRHKENTGASNSLILENLEYLIHQRANIILRYPVIPG
                     YTDSEDDVRGILSIMKRNSDILREIHLLPYHKLGQNKYRGLQASNPMPENIPDTDESV
                     GHIESIFKSNHFIVKIGG"
     gene            716..>1468
                     /locus_tag="PK976_06390"
     CDS             716..>1468
                     /locus_tag="PK976_06390"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HRC67550.1"
                     /translation="MNTKITQRIEKLREKGLRAVNRISAERALLITQFYRTAEPYRVS
                     VPVARAMAFDYILSHKKIYIDDGELIVGERGPEPKAVPTYPEVSLHSLEDLRILNTRP
                     KVSFRVDEDTFAAYRDVIIPFWKGRSNRERIMGLMDEDWLRSYEAGIFTEFQEQRAPG
                     HTVGGSKIFNRGMLDLIEDIHRVQLKLDLYEDYLAFEKNEELKAMEIAARALIHFANR
                     HADLLEELSKQEEDLQRKSELKELAQISRRVPA"
BASE COUNT          478 a          260 c          330 g          400 t
ORIGIN      
        1 attgcgatgt ttatggtgtc ataatcccga aagccagctt aacaagccgg agccattttt
       61 acggatggat aaactggaag gtaaattctt cgagcatgaa gaacaaatcg gttatgaaat
      121 cgatctggat gaattattag aaattttatt tagagatagg gcttttatgg aagaaagtgg
      181 aggtggggtt actctgagtg gaggagaacc tttgtggcaa tggaagtttt cagcagcttt
      241 agcacaaaaa ttgagcgagt caggtattca cgtagcagta gatacttgtg gttatgttca
      301 gttacgggtt ttcgaagcca tattgcctta tacccatctt tttctctatg atttaaaagg
      361 ctatgataac cacaggcaca aagaaaatac tggagcaagc aattctctca tcctcgaaaa
      421 tcttgaatat ctcattcacc agagagccaa tatcatcttg cgatatcctg taattcctgg
      481 ttataccgat agcgaagacg atgtgagagg aatactatcg atcatgaagc gaaactctga
      541 tatactgaga gaaatacatc ttttgcctta tcacaaatta ggacagaata aataccgagg
      601 cttacaagcc tcgaatccca tgcctgagaa tattcccgat acagatgaaa gtgttggtca
      661 tattgaatcg atctttaaat caaatcattt tattgtaaaa attggaggat aaaccatgaa
      721 cacaaaaata acacagcgga tcgagaaatt aagagaaaaa ggtcttagag ccgtaaacag
      781 aatctcagcc gaacgggctt tattgatcac ccagttttat cgaacggctg agccttacag
      841 agtatcggtt cctgtagccc gggctatggc tttcgattat attttaagtc acaagaagat
      901 ttatattgat gatggagaat taatcgtggg tgaacggggt ccagaaccaa aggctgtacc
      961 tacctatccc gaagtaagct tgcattctct cgaagacctt cgaattctga ataccaggcc
     1021 gaaggtatca tttcgagtgg atgaggatac ttttgcagct tatcgagatg taatcattcc
     1081 tttttggaaa ggaagaagca acagagaaag gataatggga ttaatggatg aagactggct
     1141 cagaagttat gaagctggta tttttaccga attccaggaa caaagagcac ctgggcatac
     1201 cgttggaggt tcgaaaattt tcaatagggg catgctcgat ctcattgaag acatccaccg
     1261 agtgcagttg aaactcgatt tatatgaaga ttacctggct tttgaaaaaa atgaagagtt
     1321 gaaggcaatg gaaatagccg caagggctct gattcatttt gctaatcgtc atgccgattt
     1381 actcgaagaa ttatcaaaac aagaagaaga tttacagcga aaatcagagt taaaggaatt
     1441 ggctcaaatt agccgacgcg ttcctgcc
//
LOCUS       DAORPD010000520         1466 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_17217_length_1466_cov_2.267186, whole genome shotgun sequence.
ACCESSION   DAORPD010000520 DAORPD010000000
VERSION     DAORPD010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17217_length_1466_cov_2.267186"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="PK976_06395"
     CDS             complement(<1..333)
                     /locus_tag="PK976_06395"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRC67551.1"
                     /translation="MKLVKYDIVLERLKEADIELVRQHRNSEQIRRTMEYREYITPEM
                     QREWFESVNKNIDQLYFIIHYRSEKIGLINAKNIDWEKQILESGIFLWESKYYETFLP
                     AIVSIMITD"
     gene            complement(344..1270)
                     /locus_tag="PK976_06400"
     CDS             complement(344..1270)
                     /locus_tag="PK976_06400"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRC67552.1"
                     /translation="MNQPEYSVVVPVFNSQESLLELFEGIAAAFGELNTEFETIFVDD
                     RSTDNSWEVLKNLKQEHPQHIKIIRLSRNYGQHNATLCGLMFAKGEAIITIDDDLQTP
                     PHEIITLVRSFEESSCDVIYGIPYGKKAHHFYRNLGSSVIKKSSRNLRNTPGEGSSFR
                     LLRRSITDKIVQHSLFFVFIDEILLWYTDDIRFVEVAHVPRKYRRSGYSFGKLWHLLT
                     NLVVFYTDAPLKIMVYGGLFASFVSFILGIIYIVKKLFFNVPLGYTSLIVAILFSTSI
                     ILFSLGIIGEYLSRIYKVQNRKPPFSIDTVIE"
BASE COUNT          457 a          291 c          299 g          419 t
ORIGIN      
        1 atccgtgatc atgatggaga cgatggcagg taggaaggtt tcataatatt tcgattccca
       61 aagaaaaata cccgattcaa ggatttgttt ctcccagtcg atattctttg cattaatcag
      121 gccgattttc tccgatcgat aatgaataat gaaatataac tggtcgatat tcttattgac
      181 gctttcgaac cactcacgtt gcatttcggg agtaatatat tcgcgatatt ccatagttcg
      241 acgaatctgc tcgctattgc gatgctgcct tactaactcg atatcggcct ccttcaggcg
      301 ttcgagtact atgtcgtatt ttaccaattt catcttaatt catttactct ataacggtat
      361 cgatagaaaa gggaggttta cgattttgaa ccttataaat acggctgaga tattccccaa
      421 taattcccaa actgaacagg ataatacttg ttgaaaataa aatagcaacg atcaatgagg
      481 tatatcccaa tgggacgttg aagaacaatt ttttgacgat atagataatt ccaaggataa
      541 aactcacgaa agaggcgaaa agtccaccat aaaccatgat tttgagtgga gcatcggtat
      601 aaaaaaccac cagattggtt aacaaatgcc ataattttcc aaaagagtat cccgaacgac
      661 gatattttcg aggaacatga gccacttcga caaaacgaat atcgtcggtg taccagagca
      721 agatttcatc tataaaaacg aaaaacaggg aatgttgaac aatcttatcg gtaatggaac
      781 ggcgaagcag acggaatgag gaaccttcac caggcgtgtt tcttaaattg cgtgaagatt
      841 ttttaataac agaactgcca agattcctat aaaaatgatg ggctttttta ccgtagggga
      901 taccataaat cacatcgcaa gaagattctt cgaaagatcg aaccagggta ataatttcat
      961 gaggtggagt ttgcaaatca tcgtctatgg tgataatggc ttcgcccttg gcaaacataa
     1021 gtccgcaaag tgtggcatta tgctgaccgt aatttcggga tagtcggatg attttgatat
     1081 gttgaggatg ctcttgtttg agatttttca gtacttccca gctattatcg gtgcttcggt
     1141 cgtccacgaa aatggtttca aattctgtat tcaactcacc aaaggctgcg gcaatcccct
     1201 caaagagctc caaaagagat tcctgactgt tgaaaactgg aactacgacg ctatattcag
     1261 gctgattcat aaattcgtta tcaaagatta accacctcca agaattaagc tagtagcagt
     1321 aatccaacgt gaagcatcac taaggaagaa aatgatgaca ttggccacgt ccaccggatc
     1381 acccagtcca agcggttgac gcgctttaat ttgttgaatg gtttcctcac ttgttgcttc
     1441 tcgggtttgt tcgagcatag gtcctt
//
LOCUS       DAORPD010000521         1464 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_17271_length_1464_cov_1.923350, whole genome shotgun sequence.
ACCESSION   DAORPD010000521 DAORPD010000000
VERSION     DAORPD010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1464
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17271_length_1464_cov_1.923350"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="PK976_06405"
     CDS             complement(<1..538)
                     /locus_tag="PK976_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRC67553.1"
                     /translation="MMSFLDLVKARHSTRNFENRPVEREKLECCLEAARLAPSASNSQ
                     PWTFIVVDDPGLKEEIARETYSPLVSFNKFVLKAGAIVVIVIEKPKMITQIGESIKKK
                     EFPLIDIGIAAEHFCLQAQEEGLGTCILGWFNEAPIRSLLRIPQSKTIGLLVAVGYPG
                     NEILKEKKRKPMEEFRKYN"
     gene            641..1444
                     /locus_tag="PK976_06410"
     CDS             641..1444
                     /locus_tag="PK976_06410"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HRC67554.1"
                     /translation="MCGRYILVQKLENIEKRFNVTAAFDTLLKPQYNISAGAWVPIIG
                     SDDPHHLQLMRFGITPFWSKTDKFFINARAEGDHNSENLPHYKGAKGIIEKPAFRRPI
                     RSQRCLVIADAFIEGTIQEKLNKPFVVYLRNKVRPFAMAGIWDEWKNPETGEIVRSFA
                     IITTVANELLLKLPHSRMPVILTPAEERKWLDLSLPLSDMTAMLEPYDASLMNAYPIS
                     PVIKSPKAEGAKLISPIGPPVVPEDDLQVKSYLQRSGMGNPKQNFSTKI"
BASE COUNT          409 a          322 c          282 g          451 t
ORIGIN      
        1 aattgtattt tctgaactcc tccataggct ttctcttctt ttctttaaga atttcattcc
       61 ctgggtaacc cacagctaca agaagtccaa tggttttcga ttgaggtatg cgtaaaagac
      121 ttcttatggg agcttcgttg aaccatccta aaatgcaagt tcctagtcct tcttcctgtg
      181 cttgcaagca aaaatgttcg gctgcaattc ctatatcgat cagcggaaat tctttctttt
      241 taatagattc gcctatctga gttatcattt tcggcttttc gattacaatc accacaatag
      301 ctcccgcctt caagacgaat ttattgaatg ataccagagg gctataagtt tctcttgcaa
      361 tctcttcctt caaaccagga tcatccacaa caataaaggt ccagggctgc gaattacttg
      421 ccgaaggagc gagccttgca gcttcgaggc aacattcgag cttttcacgt tccacagggc
      481 gattctcgaa attccgagtg ctatgtctcg cctttacaag atccaaaaaa ctcatcatgt
      541 ttttaatttt taatgattat cgaataaaaa tacaaattta atcataaact tttggctttt
      601 ggtttaattt tactttttca aattcaattc ttcggaaaga atgtgtggaa ggtatatttt
      661 ggtgcagaaa ctcgaaaata tcgaaaaaag gttcaatgta accgctgcat tcgatactct
      721 tttaaagcct caatacaata tttctgcagg tgcttgggtt cccattatcg gcagcgatga
      781 tcctcatcat ttgcaattga tgcgttttgg tataacgccg ttctggtcga agaccgataa
      841 atttttcatc aatgccagag ccgaaggaga tcacaatagc gaaaatttac cacattacaa
      901 gggtgcgaaa ggaattatag aaaaacctgc ctttcgcagg cctatccgct cacaacgctg
      961 cttggtgatt gccgatgctt ttatcgaagg aactatccag gaaaagttga ataagccatt
     1021 cgtagtatat ctccggaata aagttcgacc ttttgctatg gcaggtattt gggacgagtg
     1081 gaaaaatccg gaaactggag aaattgtgcg ttcttttgcc atcattacca ctgtagctaa
     1141 cgaactttta ttgaagcttc cccatagtcg catgccggtt attctaaccc ctgctgaaga
     1201 aaggaaatgg ctcgatctta gcttgccact ttccgacatg actgccatgc ttgaacccta
     1261 tgacgcttct ttgatgaatg cttatcctat aagtccggtt attaaatctc ccaaggctga
     1321 aggcgccaaa ctgatttccc caattgggcc gcctgtcgta cccgaagatg acctgcaggt
     1381 gaaatcttat ctccagcgct caggtatggg aaaccccaaa cagaattttt caacgaagat
     1441 ttgatatttt ggcattaatc ggta
//
LOCUS       DAORPD010000522         1459 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_17369_length_1459_cov_1.965812, whole genome shotgun sequence.
ACCESSION   DAORPD010000522 DAORPD010000000
VERSION     DAORPD010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17369_length_1459_cov_1.965812"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(330..911)
                     /locus_tag="PK976_06415"
     CDS             complement(330..911)
                     /locus_tag="PK976_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008774130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HRC67555.1"
                     /translation="MLSNLQDYILILASRSPRRQQLLAGLDLPFLVFTKETPEDYPLD
                     MHGKDVALMLARRKSLAFNNEDLPENFLLITADTIVWLNNHILNKPSSRQEAIIMLKQ
                     LSNNTHFVYTGICLRSSRKTETFVAESEVRFRKLEKEEIEYYVDYYSPYDKAGSYGVQ
                     EWIGYIGIEAIHGSFYNVMGLPTQILYKHLQTF"
     gene            complement(1006..>1459)
                     /gene="nadE"
                     /locus_tag="PK976_06420"
     CDS             complement(1006..>1459)
                     /gene="nadE"
                     /locus_tag="PK976_06420"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HRC67556.1"
                     /translation="HILLNTSNKSELAVGYGTLYGDMCGGLSVLGDVYKTDVYALAHY
                     INCKSPIIPESTLLKPPSAELRPNQKDSDSLPLYEILDPILRLYIEEQQGPTEIIAQG
                     HDSALVLRILKMVNSNEYKRYQAPPVLRVSKKAFGSGRRMPIVGKYLE"
BASE COUNT          443 a          276 c          285 g          455 t
ORIGIN      
        1 catgacccat gggccgcatc attcgtatca aggctttgat gcctggagca ttcactccat
       61 catcattggt aactaaaatg agtttattat ccataaacat tcctcttcaa aaataaaata
      121 atctccactt aaaataactt taccaaattt tttttcattt aaaaatgaaa ctaatgtcac
      181 catcattata aaaaagaaac atattaaata acacttcctt cggtaatttc atttttatga
      241 attaagtaat tagaaatatc gaggttcatc cttttgaatt gacaaagtta acagtattct
      301 atgataagga ttaaaattaa gttaaacaat cagaaggttt gaagatgctt gtataggatt
      361 tgagtaggca agcccatcac gttgtaaaac gaaccatgaa tagcttctat gccaatgtat
      421 ccgatccatt cctgaacgcc atacgaaccg gccttatcat atggagaata atagtcgaca
      481 taatattcga tttcttcttt ttcaagtttt ctaaatctta cctcactttc ggccacgaat
      541 gtttcggttt ttcttgatga acgcaagcaa attcccgtgt atacgaaatg agtattgtta
      601 gagagttgtt tgagcatgat gatggcctct tgtctcgaag agggtttgtt gaggatgtga
      661 ttattcagcc aaacaattgt atcggccgta atcagaagaa agttttcggg caggtcttca
      721 ttattgaaag ccaaactttt gcgtcgagca agcatcaaag cgacatcttt cccgtgcata
      781 tcgagagggt agtcttctgg agtttctttt gtaaagacca ggaagggaag gtcaagcccc
      841 gcaagcaatt gctgccttct gggtgagcgc gaagctaaaa taagaatgta atcttgtagg
      901 ttggatagca tttcgaaatt ttacctggat tgtttcaata tgattgaata tcaaaataat
      961 ggttgatcat ttctggtgaa aaacctcata aatctctcct attggctatt cgaggtattt
     1021 tccaacaata ggcattctgc gtccactacc gaacgccttc ttcgataccc tgaggacagg
     1081 aggcgcttga tatcgtttat actcgttgga atttaccatt tttagaattc gaagcaccaa
     1141 tgctgaatca tgaccctgag cgatgatttc tgtcggtcct tgctgttcct ctatatagag
     1201 acgaagaatg ggatccagaa tttcgtataa gggcaatgag tcactgtctt tctggttagg
     1261 gcgcaattct gccgaaggtg gttttaaaag ggtactctcg gggatgatag ggcttttgca
     1321 attgatataa tgagcaagag cataaacatc tgttttatag acatcgccca aaaccgataa
     1381 tcctccgcac atatcgccat aaagtgtacc ataacccacg gcaagttcgc ttttatttga
     1441 agtattgagc aaaatatgg
//
LOCUS       DAORPD010000523         1458 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_17392_length_1458_cov_2.059159, whole genome shotgun sequence.
ACCESSION   DAORPD010000523 DAORPD010000000
VERSION     DAORPD010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1458
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17392_length_1458_cov_2.059159"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(179..>1458)
                     /locus_tag="PK976_06425"
     CDS             complement(179..>1458)
                     /locus_tag="PK976_06425"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRC67557.1"
                     /translation="CILNKTIVKKNKLPKKYWASKSKNLISTFRGLFCLVMVASMIIS
                     LNDEVLALSKDGKIRTVVIDAGHGGKDPGALGKTSAEKNITLSVALKLGQYITKNHPE
                     TKVIYTRSDDSFVELYKRADIANKNKADLFISIHCNASKSRIHYGAETFVMGLHRSQA
                     NLEVAKLENAAVLYEENYQTRYDGFDPNTPETEIIFSMYQNLYRQQSLLLASNIQNQL
                     NTVASRFDRGVKEAGFLVLYRTTMPSVLVELGFISNENEENFLKSKAGQEKLATSLAS
                     AFNAYKMEVENIPAISSMAPVKPDITVDSELLSSTADQQNKTLQTSSFAQQKNTAQAI
                     SSSPTETIASTSSPIIYRVQITASTQPLSKNHSFFKGLKDIWEYKHKGYYKYTSGHFF
                     NEDDAIQYRDSLRKQGFKDAFVVPFRGDQRLNP"
BASE COUNT          391 a          280 c          308 g          479 t
ORIGIN      
        1 aatattccat ttccttttag aaaagaatat ccccaaataa atataatgat gaccccaata
       61 aaagttaatc ccgttttaaa ttctttcgat attttcaagg cagggtaatg gttttaattt
      121 taattctggt gcgaaattac aacaaattcg atcccgaata atttcattaa ctttttcatc
      181 agggattcaa tcgttgatct cctcgaaatg gaaccacgaa agcatctttg aaaccctgtt
      241 ttcgtaatga gtcacggtat tggattgcgt catcttcgtt gaaaaaatgg cccgaagtgt
      301 atttgtagta gcctttatgt ttatattccc agatatcttt taatcccttg aagaaggaat
      361 ggtttttact aagaggttgt gtagaggcgg ttatttgtac ccggtaaatt atgggcgaac
      421 ttgtggatgc gattgtttcg gttggactgg aagatatagc ttgtgcagta tttttttgct
      481 gagcgaagct cgaagtttgc aaggttttgt tctgctggtc ggccgtagat gaaagaagct
      541 ctgaatcgac cgtaatatca ggtttaacgg gggccatgct tgagatggca ggaatatttt
      601 cgacttccat tttgtaagcg ttgaaagcgc tggcaagcga cgtggccaat ttttcctgtc
      661 cggctttcga tttgaggaaa ttttcttcgt tttcgttgct gatgaatcct aattcaacta
      721 gcactgaggg catggtagta cgataaagca ccaaaaaacc agcttctttc accccacgat
      781 cgaaacgtga agctactgta ttgagctgat tttgaatgtt cgaggctaat aaaaggcttt
      841 gttgcctgta gagattctgg tacatcgaaa aaatgatttc ggtttctggg gtattagggt
      901 cgaaaccatc atatcgagtt tggtaatttt cttcgtacaa tacggcagcg ttttcgagtt
      961 tagccacctc gaggttggcc tggcttcgat gaaggcccat cacaaaagtc tcagccccgt
     1021 agtggatacg cgatttgctg gcattgcagt ggatgctgat aaacaaatca gccttatttt
     1081 tattggcaat atcggcccgt ttgtataatt ctacaaaact atcgtcgctt cttgtgtaaa
     1141 taactttcgt ctcgggatga tttttggtaa tatattgccc cagtttgaga gctaccgaca
     1201 gggtaatgtt cttttcggcg cttgtctttc ctaaagcgcc ggggtcttta ccgccatgcc
     1261 cggcatcaat gaccacagta cgaattttcc catctttcga taaggctaaa acttcatcat
     1321 tcagagaaat aatcatgctt gcaaccatca ccagacaaaa caagcccctg aaagttgaaa
     1381 ttaaattttt acttttggag gcccaatatt ttttaggcaa tttgtttttt ttcacaatcg
     1441 ttttattcaa aatacact
//
LOCUS       DAORPD010000524         1457 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_17420_length_1457_cov_1.835235, whole genome shotgun sequence.
ACCESSION   DAORPD010000524 DAORPD010000000
VERSION     DAORPD010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17420_length_1457_cov_1.835235"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="PK976_06430"
     CDS             <1..383
                     /locus_tag="PK976_06430"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC67558.1"
                     /translation="DGTSNTYPLTSISKIYFEGNNLIFNEANMPIALDQIRKITFTAS
                     SGINIVNSGNTSLVISPNPASDAIKLFNLPNETCTVYIFDLTGLLVLSCILNPSNTSI
                     NVNNLTKGIYLVKVMNSTAKFIKL"
     gene            466..>1457
                     /locus_tag="PK976_06435"
     CDS             466..>1457
                     /locus_tag="PK976_06435"
                     /inference="COORDINATES: protein motif:HMM:NF025620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HRC67559.1"
                     /translation="MKKIILLLSFLTLGTLGLFAKDYLFIFSSDKSVNAIDRADIDSI
                     KFTSNQTVLSIYKDNEVIASYPLSKIDSLTFGSIDVNEVMIIYSESGIVITNPFANKG
                     INISVNGDDVTVNSKLSNISVDYILSGTTSSGSLKLYSIYKYQLTLNGVNMTNNDGPA
                     INLQSSKMCTLVLSEGTTNILADGATYASSSEDQKATLFSEGQIIFIGDGTLNISSIS
                     KHAIASDDYISLKGGNINVSSAGKDAIHVNDYFTMSDGILQLTPSGDGIDSEGYIDIS
                     GGSITFTSSVANVKAISADSTINVCGGIINLTLKGNAAKGLKGKQDMTLSGGTFT"
BASE COUNT          464 a          296 c          269 g          428 t
ORIGIN      
        1 tggatggaac cagcaatact tatcctctta cctccatttc aaagatttat ttcgaaggca
       61 acaacctcat ttttaatgaa gccaatatgc ctattgcact cgatcaaata cgtaaaatta
      121 catttacggc ttcatcagga ataaacatcg taaattctgg taacacgagc ctcgtcatca
      181 gccccaaccc tgcgagtgat gccatcaaac tgttcaatct tcccaatgaa acatgcacgg
      241 tctatatatt tgatctgacc ggtttacttg tgttatcgtg tatcctcaat ccatccaaca
      301 catccataaa cgtaaacaat ttaaccaaag gaatttattt agtaaaagtc atgaattcga
      361 ccgctaagtt catcaaactt taaaatcttc caacaatcaa actaattaag attgtccaga
      421 acaataaagc cgataaataa tatccacatt tgaaaattct tatccatgaa gaaaataatc
      481 ctactcttat catttctcac attgggaact cttggtttat ttgctaaaga ctatttgttc
      541 atcttctcga gcgataaatc agtaaatgcg atcgatcggg cagatatcga cagcataaag
      601 tttacttcaa atcaaaccgt actttccatt tataaggata acgaagtaat tgcaagctat
      661 cccctttcga agattgacag tttgacattt ggcagtattg atgtcaatga agtgatgata
      721 atttattccg aatctggtat agtcataact aatccctttg ctaacaaagg cattaatata
      781 tcggtgaatg gtgatgatgt taccgttaat tccaaactct ccaatatctc agtcgattat
      841 attctttctg ggacaacctc cagcggttct ttgaaactct acagtattta caaataccaa
      901 cttacattaa atggggtcaa tatgaccaat aatgacggtc cggccatcaa tctgcaatcg
      961 agcaagatgt gtacactcgt gctttctgag ggcacgacaa atattctggc tgatggggca
     1021 acttatgcca gcagttcgga agatcagaaa gccacgttgt tttcggaggg tcaaattatt
     1081 tttattggag atgggacact gaatatatcg agtatttcga aacatgccat tgcaagcgat
     1141 gattacattt ccctgaaggg tggaaatatc aatgttagtt cagctgggaa agatgccatt
     1201 cacgtgaacg attatttcac aatgagtgat gggattcttc aactcactcc ttcgggtgat
     1261 ggaatcgatt cagaaggata catcgatata tcaggcggga gtattacttt cacgagttct
     1321 gtggcaaatg taaaagccat ttcagcagat agcactatca acgtttgcgg aggtatcatc
     1381 aatcttactt taaaggggaa tgcagccaag gggctaaagg gcaagcaaga catgacgctt
     1441 tcggggggaa cattcac
//
LOCUS       DAORPD010000525         1456 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_17439_length_1456_cov_2.443255, whole genome shotgun sequence.
ACCESSION   DAORPD010000525 DAORPD010000000
VERSION     DAORPD010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17439_length_1456_cov_2.443255"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..898
                     /locus_tag="PK976_06440"
     CDS             <1..898
                     /locus_tag="PK976_06440"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRC67560.1"
                     /translation="VSEPISSLPLSLKPLGYHSIFYHGGMNGTMHFDAFSRLSGFDEY
                     KGKNEYRGPEAFDGRWGIFDEPFFQFMAKDLSNQTQPFLVVFFSLSSHHPYVVPAKYM
                     NRFGPGKLKIHQSIRYADYALGQFFKMASQESWFKNTLFIITADHTSEAFSPYYLTPV
                     GQHVIPLIFYLPDNSFRGFSSQIAQQCDIYPSVMDFLNAEGSFVAFGRSVFDITSTPF
                     GISYLNQEYQLIYKNKCLRYFNEKVSGFYDLKNDSLQTNNLAAEQLPEQDEALRFLKA
                     IIQQYNNRIIYHRLRADQMVKP"
     gene            895..>1456
                     /locus_tag="PK976_06445"
     CDS             895..>1456
                     /locus_tag="PK976_06445"
                     /inference="COORDINATES: protein motif:HMM:NF012981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HRC67561.1"
                     /translation="MKKIYFIINPAAGIHHSRKQKLEEMIPEYFPAETFQTEIVRINE
                     KDRISDLTQKAVAKGYDIIVAVGGDGTINEIAQVLVDSSAVLGIIPAGSGNGLAHFLK
                     IPFKLEEALNLIKNGEAMCIDTIQLNDRIAVSIAGMGFDARIARQFALDNHRGFFTYF
                     KIAVKEYITFRPRLFQVITNNFIIKER"
BASE COUNT          433 a          305 c          291 g          427 t
ORIGIN      
        1 tgttagcgag cctatctcga gcttgccttt aagcctgaag cctctgggat atcattccat
       61 attttatcat ggaggaatga atggcaccat gcatttcgat gcattttcgc gtttgtctgg
      121 tttcgatgaa tataaaggta agaatgaata caggggacca gaagcctttg atggaagatg
      181 gggaattttt gacgagccct ttttccagtt catggcgaag gatctctcga atcaaacaca
      241 gccatttctg gtagtattct tcagtttgtc ttcgcatcat ccttatgtcg ttccagcaaa
      301 atatatgaat cgtttcggac ctgggaaact caagatacat caaagcatac gttatgccga
      361 ctatgcattg ggtcaattct tcaaaatggc ctcgcaggaa tcatggttca agaataccct
      421 tttcatcatt acagccgacc acacctccga ggcttttagc ccttattatc ttacaccggt
      481 tgggcaacat gtcattcctt tgatcttcta tctgcccgat aattctttcc gcggcttttc
      541 atcgcagatt gcgcagcaat gtgatattta tccttcggta atggatttct taaatgctga
      601 gggttcgttt gttgcctttg gtcgaagtgt tttcgatatc acctctaccc catttggtat
      661 cagctatcta aatcaagaat atcaactaat ttataagaat aaatgcctca gatatttcaa
      721 tgaaaaagta agtgggttct acgatttaaa aaacgacagt cttcaaacca ataaccttgc
      781 tgctgagcaa ttgcctgaac aggacgaagc tttacgtttt ctaaaagcta tcatacagca
      841 atataacaac cggataatat atcatcgact ccgcgcagat caaatggtaa aaccatgaaa
      901 aagatctact tcatcatcaa tcccgctgca ggcattcatc attcgcgtaa acaaaaatta
      961 gaagagatga tacccgaata ttttccggcc gaaacctttc aaacagaaat cgttagaatt
     1021 aatgaaaagg acagaatttc ggatcttacg caaaaagccg ttgcaaaagg ttatgatatc
     1081 atcgtagcgg taggaggaga tggaaccata aacgaaattg cacaagtact ggtagatagc
     1141 agtgctgttc ttggcatcat cccggctggt tcgggtaatg gtctggcaca ttttctcaag
     1201 attcctttca aacttgaaga agcattgaat ttgataaaaa atggagaagc catgtgcatc
     1261 gacaccattc agctaaacga tcgtattgcg gtaagcatag caggtatggg cttcgatgcg
     1321 cgtattgcaa ggcaatttgc cttagacaat catcgaggat tttttactta tttcaagatt
     1381 gcggtgaagg aatacatcac ttttcgcccg cgactcttcc aagttattac caacaatttc
     1441 atcatcaagg aaagag
//
LOCUS       DAORPD010000526         1456 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_17443_length_1456_cov_2.174875, whole genome shotgun sequence.
ACCESSION   DAORPD010000526 DAORPD010000000
VERSION     DAORPD010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17443_length_1456_cov_2.174875"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..1363
                     /locus_tag="PK976_06450"
     CDS             158..1363
                     /locus_tag="PK976_06450"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRC67562.1"
                     /translation="MDILFLCNKPPFPAREGGPIAMNMLIEGLIEQGHQVKVLTVSSY
                     KYPFRPELIPPDYMEATCIEAVEVDLHIRPFKAFINLFARKSYHVERFISKTFRRRLI
                     NILQEHEFDIVQLETVFLCPYLPDIRNHSMARIVLRTHNIEHLIWKRLWENSKPGPKK
                     VYLKHLWTTLRKYELGILDKADGIVAITRKDAEFLKPFTRVPIIDISFGIDSSQFPEP
                     TYDDCEIPSLFHIGSMDWIPNQEGIQWFLSEVWPKVYDNFPFLKFYLAGRNMPEWLLN
                     GFWPNVVVLGEVEDARKFMLSKWIMVVPLLAGSGIRVKIIEAMANGRPVIASPIGAEG
                     IEYTEGENILLASTPDQFLEAIRKCIDNPQFAIQLGRNAHLLVMKEHDRRKLITELTH
                     FYHRLVYTP"
BASE COUNT          427 a          306 c          294 g          429 t
ORIGIN      
        1 ggcttacatg gaaggctatt tccgacagtc ctattctcga catggatact tataaccgat
       61 ttttcaacca ttttattcca aaaaaatgga agctcccctt atcagaacaa gtataaactg
      121 agaatcatca acacttcagt atgcgaatag aataaaaatg gatatactct ttttatgcaa
      181 caagccaccg tttccagccc gtgagggagg tcccatagca atgaatatgc tcatagaagg
      241 actgatcgaa caggggcatc aggtgaaagt tttgacagtt agctcttata aatatccctt
      301 tcgtccggaa ttaattcctc ccgactatat ggaagctacc tgcatagagg cagtcgaagt
      361 ggatcttcat attcgcccct ttaaagcttt tatcaattta ttcgccagaa aatcttatca
      421 cgtagaacgt tttatttcga agactttccg ccggagactt atcaatattc tccaagagca
      481 tgaattcgac atcgtccaac tggaaaccgt ctttttatgt ccctacctcc cggatataag
      541 gaatcattcg atggcccgta tcgtattgcg gacgcacaac atcgaacacc ttatttggaa
      601 gcgattatgg gaaaacagca agcctggacc gaaaaaagtc tatcttaaac atctctggac
      661 aactttaagg aaatacgagt tggggattct agataaagca gatggaattg ttgccattac
      721 tcgcaaagat gccgaattcc ttaaaccatt tacacgggtt cccatcattg atatttcgtt
      781 tgggatagat agttcgcaat ttcctgaacc tacttatgac gattgtgaaa tcccttcgct
      841 ttttcatatt ggcagcatgg attggatacc gaatcaggaa ggtattcaat ggtttctgtc
      901 agaagtatgg ccaaaagtgt acgataattt tccttttttg aaattttatc ttgctggccg
      961 aaatatgccc gaatggcttt tgaatggttt ctggcctaat gttgtagttt tgggtgaagt
     1021 ggaagatgcc cgaaaattca tgctgtcgaa gtggattatg gttgttcctt tgctggccgg
     1081 tagtggaatt cgggtgaaaa tcatagaagc tatggctaat ggcaggccgg ttattgcctc
     1141 tcccattgga gccgaaggga tagaatatac agaaggggaa aatatcctgc tggcttctac
     1201 tcccgaccaa tttctcgaag ccattcgaaa atgcatagat aacccacagt ttgctatcca
     1261 gttgggacgt aatgcacact tactcgtcat gaaagaacat gacagacgaa aactcatcac
     1321 cgaactcact catttttacc atcgccttgt atatactcct taacctatat tcgccctttc
     1381 tgattttttc attcgaccaa ggactcgtaa ttttgcaaga atgcaacaat gaataaagga
     1441 tgaaattact ggtgat
//
LOCUS       DAORPD010000527         1455 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_17462_length_1455_cov_2.475000, whole genome shotgun sequence.
ACCESSION   DAORPD010000527 DAORPD010000000
VERSION     DAORPD010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17462_length_1455_cov_2.475000"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="PK976_06455"
     CDS             <1..745
                     /locus_tag="PK976_06455"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRC67563.1"
                     /translation="KILTDLQNRDDNIYRVIFETEPVPASIRQAGIGGANRYQNFEGS
                     DNFALLLETAKKLDRVKSQIYVQSKSFDEVFEMAKKKEAMVTAIPAIQPVSKRDLKYI
                     SSYFGYRIHPFYKTVMFHEGIDFTAPKGAKVYATGDGVVKEASLINLDGFGKKIIIEH
                     GFGYQTIYAHLNSFNVKPGQKVKRGDIIATVGTSGLSTGPHLHYEVIYNHQKVNPAYF
                     MFDLDPATFQEIIRQSSDYKESSNIPVGE"
     gene            777..1118
                     /locus_tag="PK976_06460"
     CDS             777..1118
                     /locus_tag="PK976_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRC67564.1"
                     /translation="MTSQNNQKKKQHLYYTIGEVSRMLDVNPSTLRYWEKEFDGIHPY
                     RNRKGTRFYHTEDVETLRLIKYLLKERGFTIAGARDYLKTSRSHVLRNMDIVETLKNI
                     REFLIQLRNNL"
     gene            1181..>1455
                     /locus_tag="PK976_06465"
     CDS             1181..>1455
                     /locus_tag="PK976_06465"
                     /inference="COORDINATES: protein motif:HMM:NF022317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 family protein"
                     /protein_id="HRC67565.1"
                     /translation="MIFNLIGWCFTKLRKLNLITLLLTASMWTIAGIWYGLGYCPLTD
                     WHYDVLRLLGYQNLPNSYLQFITHRLTGINLSTPVADIITVSGLILS"
BASE COUNT          450 a          300 c          276 g          429 t
ORIGIN      
        1 aaagattttg acagaccttc aaaaccgtga cgacaatatc taccgcgtaa ttttcgaaac
       61 agaaccagtt ccggccagta taagacaagc aggaataggc ggggcaaacc gttatcagaa
      121 tttcgaaggc agtgataatt ttgccttgct attagaaacc gctaaaaaac tcgaccgggt
      181 aaagagccag atctatgtgc agtcgaaatc cttcgatgag gtctttgaaa tggccaagaa
      241 aaaggaagct atggttacgg ctattcctgc aatacaaccg gtaagcaaac gtgatttgaa
      301 atatatttct tcatattttg gttacagaat ccatcctttc tataaaaccg tgatgtttca
      361 tgaaggcatt gactttaccg ctcccaaagg agccaaagtt tacgctacgg gtgatggagt
      421 ggtaaaagaa gcttctttaa tcaatctgga tggatttggc aaaaaaatta tcatcgaaca
      481 cggatttggt tatcaaacga tttatgctca tctgaattca ttcaatgtaa aacctggtca
      541 aaaggtaaaa cgcggtgata taattgccac tgtgggaacc agcgggcttt caactggacc
      601 acacctgcac tacgaagtca tttacaacca ccagaaagta aacccggcct attttatgtt
      661 cgacctcgat ccggctacct tccaggaaat tatccgtcaa tcatcggatt acaaagaatc
      721 ttcaaatata cctgtgggtg aataattccc tttcatcttt atctcttact ctattcatga
      781 cttcccaaaa caatcagaaa aaaaaacagc atctttatta tacgattggt gaagtaagca
      841 gaatgctcga cgtaaatcca agtacgctga ggtattggga aaaggaattc gatggtattc
      901 atccatatcg caaccgtaaa ggaactcgtt tctatcatac ggaagatgtg gaaacgcttc
      961 gattaattaa atatttactc aaagaaagag gatttactat tgcaggagca cgcgattacc
     1021 tgaaaaccag tcgaagccat gtcctaagaa acatggacat tgtcgaaacc ctgaaaaata
     1081 tcagggaatt tcttatccag cttcgcaata atctttaagg tgttctatgt ccttttatca
     1141 tctactcgac tggtttttta ccattttcca ttcgtctctg atgatattca atcttatagg
     1201 atggtgtttt actaaactgc gtaagctgaa cctcattact ttgttactta ctgcttcaat
     1261 gtggacaatt gcaggcattt ggtatggctt gggttactgc ccgcttaccg attggcatta
     1321 tgatgttttg agattactgg gttatcaaaa ccttccaaat tcttatctcc aattcattac
     1381 acatcgactt acaggcataa acctcagtac gcccgttgca gatatcatta ccgtgagtgg
     1441 tcttatttta tcttt
//
LOCUS       DAORPD010000528         1453 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_17508_length_1453_cov_2.020029, whole genome shotgun sequence.
ACCESSION   DAORPD010000528 DAORPD010000000
VERSION     DAORPD010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17508_length_1453_cov_2.020029"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..904
                     /locus_tag="PK976_06470"
     CDS             <1..904
                     /locus_tag="PK976_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67566.1"
                     /translation="QGNASIDFYDLDTKEVIHDVFFTANQRPLGDVAQSLNKVGNKIW
                     IVVNNSGKIEIVKSKNFESIMTLTGFASPRYLLFHKNGKAYLSDLYKESLYVIDTLTG
                     QTIKEISTGKSCEYLIEAGSHIFALNWSSLNDSLNNTLTIIDGLADSVVKSVILTKEP
                     NSAAIDKNGHIWILCSGGFFFEETPALYCMNPQGEILKKFEFSGNQTYPTRLCINPSH
                     DTLFYLNKDVYRMGIMENDLPQLPFIQSNNKNFYGLNAGNFILLSDPLDYQQNGNVYV
                     YSRKGQLLSTFQAGMVPSGFQWNE"
     gene            931..1347
                     /locus_tag="PK976_06475"
     CDS             931..1347
                     /locus_tag="PK976_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HRC67567.1"
                     /translation="MSDKVSLNWMEDLAFEANVNGHKIIMDAVESAGGRNRGPRPKPL
                     VLAALAGCTAMDVIPILRKMQVIPEGFGIEVEAETTEEHPKVYHTIKLIYIFKGQNLP
                     IDKLQKAIDLSQERCCGVSAMLRKAAHISYEMRIEN"
BASE COUNT          472 a          277 c          280 g          424 t
ORIGIN      
        1 gcaaggaaat gcaagtatcg atttttatga tttggacaca aaagaagtta ttcatgacgt
       61 atttttcact gccaatcagc gaccattggg tgatgtggca caaagcctga acaaagtggg
      121 aaataaaata tggattgtgg tgaataattc cggcaaaatc gaaatcgtaa agtcgaaaaa
      181 tttcgaatcg ataatgacac taactggttt cgcatcacct cgttatcttt tatttcacaa
      241 aaatggcaag gcctatctca gtgatctcta caaggaatct ctttacgtaa tcgatacctt
      301 gacgggacaa acaatcaagg aaatatccac cggaaaatcc tgtgaatatc tcatcgaagc
      361 tggtagtcat atttttgcac tcaactggtc atcattgaat gatagcctca acaatacgct
      421 tactattata gatggcttgg ccgatagtgt ggtaaaatcg gttatcctta ccaaggagcc
      481 caatagtgct gccatcgata aaaatggcca catctggatt ttatgcagtg gaggattttt
      541 tttcgaagaa actccggcac tatattgtat gaatcctcag ggtgaaattc tgaaaaaatt
      601 tgaattttcg ggtaatcaga cctatcctac tcgtttgtgc attaacccaa gccatgatac
      661 tttattttat ttgaacaaag atgtgtaccg tatgggaatt atggagaatg atcttccgca
      721 attaccattc attcaatcta ataacaagaa tttctacggt ttgaatgcag gaaacttcat
      781 tttactgtcc gatcctttgg attatcaaca aaacggcaat gtttacgttt attcgagaaa
      841 gggacaactt ttatccactt ttcaagctgg tatggttcct tcaggttttc aatggaatga
      901 ataactttgc aaaaaataaa atttagatct atgagtgata aagtctcttt gaactggatg
      961 gaagacttag ccttcgaagc caatgtaaac gggcataaaa tcattatgga tgcagtagaa
     1021 agcgcagggg gacgtaatcg tggaccccgc cctaaacccc ttgttctggc ggctctggca
     1081 ggatgtaccg ccatggacgt aattccaatt ttgcgcaaaa tgcaggtaat tcctgaagga
     1141 tttggaatcg aagtagaggc agaaactact gaagaacacc caaaagtata tcataccatc
     1201 aaattgattt acatattcaa aggtcaaaat ttgcccatcg ataaactcca gaaagccatc
     1261 gacctttcgc aagaacgctg ttgtggcgtt tcagccatgc tcagaaaagc tgcccacatt
     1321 tcctacgaaa tgcgtataga aaattaagcc ataaagaaaa caatttttct ttgctttgtt
     1381 ttgctaagac gtaataaaca ttacaggaaa ggatatgaat caataaattt cctatttcat
     1441 gattacctta tct
//
LOCUS       DAORPD010000529         1452 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_17524_length_1452_cov_2.312813, whole genome shotgun sequence.
ACCESSION   DAORPD010000529 DAORPD010000000
VERSION     DAORPD010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17524_length_1452_cov_2.312813"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1452
                     /gene="infB"
                     /locus_tag="PK976_06480"
     CDS             <1..>1452
                     /gene="infB"
                     /locus_tag="PK976_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009138014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HRC67568.1"
                     /translation="DLGLFVSINQRIDAETISIIASEFGYEVAFSEEEILEDSEQRFE
                     DRPEDYLPRHPIVTVMGHVDHGKTKLLDYIRNSNVIAGEYGGITQHIGAYEVTLSNGK
                     KITFLDTPGHEAFTAMRARGAKITDIAIIVVAADDGVMPQTKEAINHALAAGVPLVFA
                     INKMDKPTANPTRVKEELASLNILVEDWGGKYQCQEISAKTGQNVDLLLEKVLLEAEL
                     LDLKANPKRPAIGTVIESSLDKGRGYIAKILVQNGILHQGDIIVAGNVYGKIKAMYNE
                     RNQFVKEVGPSSPILILGLSGAPQAGDKFNVFLDDKEAKAVANKRQQLQREMEIRTQK
                     HITLDEIGRRIAIGDFKELNLIVKGDVDGSVEALSDALLKLSTDEVQVNVIHKSVGQV
                     TETDVLLASASNAIIVGFQVRPSANAKKLAEQEQIDIRIYSIIYQAIDEIKAAIEGMM
                     APKIEEKIVCNLEVKEIFNISKIGTVAGCLVLD"
BASE COUNT          457 a          299 c          326 g          370 t
ORIGIN      
        1 tcgacttagg tctgtttgtc tcgataaatc aaaggattga tgcagaaact atcagtatca
       61 tagcttctga atttggatat gaagtagcct tttcggaaga agagatctta gaagattcag
      121 agcaaagatt cgaagatcga cccgaagatt atttgccacg acaccctatc gttactgtga
      181 tgggccatgt tgaccacggc aaaaccaaac ttctcgacta tatccgcaac tcaaatgtaa
      241 ttgcaggaga atatggcggt ataactcaac acattggggc ttatgaagta acattaagca
      301 atgggaaaaa aattaccttt ctcgatactc ctggtcatga agcctttaca gccatgcgtg
      361 cacgtggtgc aaaaattacc gatattgcca ttatagttgt agctgccgac gacggggtaa
      421 tgcctcaaac caaagaagcc atcaaccatg cactagcagc aggtgttcct ttggtgttcg
      481 ctatcaataa gatggataaa cctacagcca atcctaccag agttaaagaa gaacttgcca
      541 gtttgaatat tcttgtagaa gattggggag ggaaatatca gtgtcaggaa atttctgcaa
      601 aaaccggtca aaatgttgac ttgcttctgg aaaaagtctt gcttgaagcc gagttactcg
      661 atctgaaggc caatcctaaa cggccggcta ttggtacggt cattgaatcc agcctcgata
      721 aaggccgtgg ctacatagcc aaaattcttg tccaaaatgg catacttcat cagggcgata
      781 ttattgttgc cggtaatgta tatggaaaaa taaaggcaat gtacaacgaa cgcaaccagt
      841 tcgtaaaaga agttgggccc tcctctccca ttctcattct tggactcagt ggtgccccac
      901 aggcaggcga caaattcaac gtgttcctcg atgataagga agctaaagca gttgctaata
      961 aacgtcagca acttcaacgt gaaatggaga ttaggacaca gaaacatatt accctcgatg
     1021 aaattggccg tagaatagcc attggcgatt tcaaagaact caacctcatt gtaaagggcg
     1081 atgtagatgg ctcggttgaa gctctttctg acgccttgtt gaaattatct accgatgagg
     1141 tgcaggtaaa tgtcattcat aaatcggtag gtcaggttac cgaaaccgac gttttgctgg
     1201 cgtcggcctc caatgcaatt attgtgggat ttcaggtacg tccttcggcc aatgctaaaa
     1261 aattagccga gcaggaacaa atagacatca ggatctattc cattatctat caagctatag
     1321 atgaaataaa agccgctatc gaaggaatga tggcacccaa aatcgaagaa aaaatagttt
     1381 gtaatctcga ggtaaaggaa attttcaata tcagcaagat agggacggta gccggatgcc
     1441 tcgtcctcga tg
//
LOCUS       DAORPD010000530         1452 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_17529_length_1452_cov_1.719399, whole genome shotgun sequence.
ACCESSION   DAORPD010000530 DAORPD010000000
VERSION     DAORPD010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17529_length_1452_cov_1.719399"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="PK976_06485"
     CDS             complement(<1..192)
                     /locus_tag="PK976_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HRC67569.1"
                     /translation="MKIIVLNCGSSSIKYQLFNMPSAEVHAKGLVDKIGLKGASIKHQ
                     RNDGLEFKVEGEILDHQKGI"
     gene            complement(205..1188)
                     /locus_tag="PK976_06490"
     CDS             complement(205..1188)
                     /locus_tag="PK976_06490"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HRC67570.1"
                     /translation="MDIKLEEFSLSKDESQKGLLQKVGIVGCGIMGQQIALQTSQYGI
                     DVIGLDVDQTRIEEITRCLDDELNRIINHWGMTAAEKKLILSRITLTTHYEDLNDCDI
                     AIETIKSKKPGTSLSMRQEVFHKLEEVLRHDAVIASNTATLMISDLSSVLKDPSRAVG
                     MHFISPVREVKIVETVRSMRTSKDTWDLACKYIRMIGKKVIPVNESPGNISTRMIIPF
                     INEACSILMEGVAMVDQIDETMREASGHMMGPFEMADTIGLDKIMRWMENLYNEFGEI
                     RYKPSPLLKRMVRAGMVGQRVGEGFYKWTPKGKQIKEGPVFTLGRESFFNT"
BASE COUNT          392 a          335 c          243 g          482 t
ORIGIN      
        1 gattcctttc tggtgatcga gtatctctcc ttccaccttg aattccaatc catcgttgcg
       61 ttggtgtttt atagaagctc ccttgagccc tatcttatct accaggcctt tggcgtgcac
      121 ttcggccgaa ggcatattga acagctgata tttaatggaa gaacttccgc aattcaaaac
      181 aattattttc ataaatataa ttcgttacgt attaaaaaat gattctcttc ccagagtaaa
      241 aactggccct tccttaattt gtttcccctt tggagtccat ttgtaaaatc cttcacccac
      301 gcgttgtcca accattccgg cacgaaccat tcgcttgagc aagggcgaag gtttatagcg
      361 aatttcacca aattcgttgt ataaattttc catccagcgc atgatcttgt ctaaacctat
      421 ggtatcggcc atttcgaaag ggcccatcat gtgtccgctg gcttcgcgca tggtttcatc
      481 gatttgatct accatggcta ccccctccat caaaatggaa caggcttcat tgataaacgg
      541 tatgatcatg cgagtgctga tattgccagg tgattcattc acgggaatta cttttttccc
      601 aatcatcctg atatacttgc aggccaaatc ccatgtatct ttcgaagtac gcatagaccg
      661 tacggtttct acaatcttta cttcccttac aggtgaaatg aaatgcatgc cgacagccct
      721 gctggggtct ttcagtacag atgaaagatc agaaatcatc aaggtggcag tattactcgc
      781 tataacagca tcatgacgca atacttcttc taatttgtga aatacctctt gcctcatact
      841 aaggctcgta ccaggctttt tcgatttaat cgtctcgatt gctatatcac agtcgtttaa
      901 atcttcgtaa tgggtagtaa gtgtaatcct cgacaaaatg agctttttct cggcagccgt
      961 cataccccaa tgattaatga ttcgattgag ctcatcatcc aaacatcgtg taatctcttc
     1021 aattcgagtc tgatcaacat ctaacccgat tacatcgatc ccatactgac tcgtttgtaa
     1081 agctatttgc tgccccatga tgccgcagcc tacaatccca actttctgta ataagccttt
     1141 ttgcgattca tctttgctca ggctaaattc ttctaacttt atatccatcg aaatcagttt
     1201 gaataagttg acagtaccat ctttttcact ggcaaaagta cagttttacc atggggtatc
     1261 aaaatgattt tataagtttc tatttcagta ctaactttgt cggttggctc tataaaatat
     1321 tctgaaattt aacattttcg attttcgaat aaatcataaa aaccttaaac ttatgtcgaa
     1381 tcatcgtttt acagtttttc tctttctttt ttgctcgatt ttttcacaaa atgtaaaaag
     1441 cgaagacatt cg
//
LOCUS       DAORPD010000531         1451 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_17545_length_1451_cov_2.312321, whole genome shotgun sequence.
ACCESSION   DAORPD010000531 DAORPD010000000
VERSION     DAORPD010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17545_length_1451_cov_2.312321"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="PK976_06495"
     CDS             <1..640
                     /locus_tag="PK976_06495"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HRC67571.1"
                     /translation="ISGGIAALQIEQPMLLWLFVLSFGLLWYYSAYLKRKPFWGNFLV
                     SFLSALVIITPWLFEFYALLLKPDEWIAFYRFYPHVNYIILSLSIFAFISTLIREIIK
                     DAEDMEGDQTYECQTLPIILGWETTHRILQALIALMMILLAMAQYWLRSHHYNLAFYW
                     YFTVQFLSIRLLTVLPADVNKNQLHRCSSLAKITMLAGILGILALIPPLFST"
     gene            690..1094
                     /locus_tag="PK976_06500"
     CDS             690..1094
                     /locus_tag="PK976_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HRC67572.1"
                     /translation="MIEIFNKALISRFIKFIIVGFSGLIVDYGITAFSKEILKIQRFI
                     SNAMGFICAASSNYYLNRIWTFSSTNPEVVREFGFFFVISLIGLGINSLILWFVLKKF
                     KLNFYLAKIFAIAGATLWNFFANAFITFQMPI"
BASE COUNT          416 a          276 c          249 g          510 t
ORIGIN      
        1 gatttcaggg ggaatagcag ctctacagat agagcaacct atgctcttat ggttgtttgt
       61 tttatcgttc ggtctgcttt ggtattactc ggcctatcta aaacgaaaac ccttctgggg
      121 aaattttctc gtgtctttcc tctcggcttt agtcattatt acaccttggc ttttcgaatt
      181 ttatgcactg ttgcttaagc ctgacgaatg gattgcgttt tatcgtttct atcctcatgt
      241 aaattatatt atactgtcgt tgagtatttt cgcttttatt agcacattga taagagaaat
      301 tatcaaggac gccgaagaca tggaaggcga tcaaacttat gaatgccaaa ccctgcccat
      361 tattcttgga tgggaaacta cgcacagaat actgcaggct ctcattgctt tgatgatgat
      421 tctgttagca atggcgcaat attggcttcg atcccaccat tataaccttg cattttactg
      481 gtatttcacg gttcaattct tatcgatccg tttgcttacg gtgctccccg ccgatgtcaa
      541 caaaaaccag ttgcatcgct gttcatccct tgctaagatt actatgctgg cgggaatact
      601 tgggatcctt gcactgatcc ctcctttgtt ttcgacataa cacactatct atgaataaat
      661 tatcaaatct ttgattaatc cgattaatta tgatagagat attcaacaaa gccttgatca
      721 gcagattcat aaagtttata atcgtgggat tttcaggact tattgttgac tatggtatta
      781 cagcattttc gaaggaaatt ttaaaaatac agcgcttcat atctaatgca atgggattta
      841 tttgtgcagc ctcatcgaac tactatctga accgaatctg gaccttttcg agcactaacc
      901 cagaagttgt tagagaattc ggcttctttt tcgttatttc attgataggt ttgggtatta
      961 atagccttat tctatggttt gtccttaaaa aattcaaact gaacttttac cttgcaaaga
     1021 tctttgctat tgcgggagca acactttgga attttttcgc caatgctttt attacctttc
     1081 agatgccaat ctaattaacc atctcctgcc cttccaaaat gttatatttt cttttacaac
     1141 gctgtcagat atgttcattt ctatatattt tacccggcta taactattta aataacacat
     1201 tttcgataag gatgagaaat cagatatgga tttttcctga attgttataa ataattcaaa
     1261 agaaatttca gtttaatatc tgtaattaaa aaaccgatac ttgttttctc ccaattcgat
     1321 ctttccctac ttattatgaa ttgaaaaaat tctaacggac atggttggag aaatgaaatg
     1381 agttggataa cagtaagtca tcagaagaaa aaacttcatt ctgttcttga attcaaaaaa
     1441 gaggcaggaa a
//
LOCUS       DAORPD010000532         1443 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_17723_length_1443_cov_2.197406, whole genome shotgun sequence.
ACCESSION   DAORPD010000532 DAORPD010000000
VERSION     DAORPD010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17723_length_1443_cov_2.197406"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="PK976_06505"
     CDS             complement(<1..315)
                     /locus_tag="PK976_06505"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRC67573.1"
                     /translation="MRKLYMLTLLLFGLSFGVMAQQVITIKGQVKNKKDGTTIPGVTV
                     LLKGTTTGTVTDIDGNYMLNAPNNGILVFSFVGMKVIEEPIQGRSRIDIEMEPMISQL
                     EEV"
     gene            740..>1443
                     /locus_tag="PK976_06510"
     CDS             740..>1443
                     /locus_tag="PK976_06510"
                     /inference="COORDINATES: protein motif:HMM:NF040269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutT"
                     /protein_id="HRC67574.1"
                     /translation="MDPNVIPNVSVDCVVIGYDLQDISILLIEREMVYNGKKYIDLKL
                     PGDLVRWDEDIDSSAYRILFDLSGVQISNLKQLQAFGEVDRLKKEPRDMEWLRAIDHP
                     EERVITVAYYTLVSLDLIHSQKFKPNPNARWVPISKIGKLAFDHNRIINKALDTLRQE
                     IKQTPVAFDLLPRKFTLSQMQKLYEVVMGTLFDKRNFRKKIAQIPCIVPINEKQVNVP
                     HKPARYYMFSRDVYEVT"
BASE COUNT          451 a          244 c          279 g          469 t
ORIGIN      
        1 cacttcttcc aactgagaaa tcatgggttc catttctata tcaatgcgac ttctcccctg
       61 gataggttct tctatgactt tcatccccac aaaactgaat accagaatcc cgttgttggg
      121 ggcattgagc atgtaattcc cgtcaatgtc agtaacagtt ccagttgtag tgccttttaa
      181 aagaaccgtt accccgggta tggtcgttcc atccttctta tttttgacct gtcccttgat
      241 ggtgataacc tgctgggcca tcaccccaaa actgaggcca aataacaaca gcgtaagcat
      301 gtagagcttt ctcatattcg tttaatttaa gtattaaagg tcgaatcgaa tcataaaaat
      361 aagcaatttt cggttttgaa taacatttca gaataaattt ttgttctttc atgcaatgga
      421 atgaatgtat tcgagtcaca tcgagtaagg gtcgatttaa aggattagtg taaattgaac
      481 acaaatacaa aaatagttta taatttgaat ttatcgatca tgatgttaaa aattcttttt
      541 aattcgaatg aaatgtggtg ctttagagaa ttttcatttc gatgaaatgt tataaatgta
      601 ttgtatgcaa tgtgttcgtt tgattgtttt tcgatcgaat aaattaaaat gatttgtttg
      661 gatagattga aaatctttat atttgtaggc tgagttttaa gcaagtgtat tagttacaca
      721 aaacatcagt catggcaata tggacccaaa tgtaatccct aatgtttcgg ttgattgtgt
      781 ggtaattgga tatgatctgc aggatatcag tattttactg atagaaagag aaatggttta
      841 taatgggaaa aagtatatcg atttaaaact tccgggtgat ctggtaagat gggatgaaga
      901 cattgatagc tcagcctatc gtattttatt tgatttatcg ggtgtacaaa tcagtaattt
      961 gaagcagctt caggcctttg gtgaggtaga tcgcttgaag aaagagccaa gagatatgga
     1021 atggttacgt gctatcgatc atcccgaaga aagggtaatt accgtagcct attacacttt
     1081 ggtaagtctt gatcttattc attctcaaaa attcaaacct aatcctaatg cgcgctgggt
     1141 gcctatatcg aaaatcggta agcttgcgtt cgatcataac cgtatcatta ataaagcctt
     1201 agatacattg cgtcaggaaa taaaacaaac tcccgtggcc ttcgatctat tgccgcgcaa
     1261 atttactctt tcgcaaatgc aaaaactcta tgaagtagtg atgggtacat tattcgacaa
     1321 acgcaatttc agaaagaaaa tagctcagat accctgtata gttcctatta atgaaaaaca
     1381 agtcaatgta cctcacaaac cagctcgtta ttatatgttt agccgcgatg tttatgaggt
     1441 gac
//
LOCUS       DAORPD010000533         1442 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_17743_length_1442_cov_2.431146, whole genome shotgun sequence.
ACCESSION   DAORPD010000533 DAORPD010000000
VERSION     DAORPD010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17743_length_1442_cov_2.431146"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..830
                     /locus_tag="PK976_06515"
     CDS             <1..830
                     /locus_tag="PK976_06515"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRC67575.1"
                     /translation="QTGAITPESRFYCNGKASLPIACSHSHYSPLELIGAIEQSCNPY
                     FWGVFKTTIEKTGGGDIHKGYNIWRQNVMSLGFGNYFDTDIPNQVKGNIPSSQYYDRY
                     FGEKGWKALTIRSLAIGQGEILITPLQLANQAVVIANKGYYYPPHVVKSVQGKPTRWN
                     KKQSSIDSQHFETITKGMYRVFQGVSGTGRWYKLENIDICGKTGTVQNPHGKDHSLCI
                     AFAPAENPKIAISVVIENAGFGATWAVPIASLMIEKYLTGKISRTELEERMSQANLL"
     gene            827..>1442
                     /gene="rodA"
                     /locus_tag="PK976_06520"
     CDS             827..>1442
                     /gene="rodA"
                     /locus_tag="PK976_06520"
                     /inference="COORDINATES: protein motif:HMM:NF037961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HRC67576.1"
                     /translation="MNTTRYKYHNPFAGIDRTLLWLYFTLVIFGWLNIYAAVYDEIHK
                     NIFDLQTNYGRQMIWIATSFVIAMAILLTDVKLFNSFAFFIYGLVIVSLILVLFVGKE
                     ISGSKSWLQIGPISIQPGEFAKFATNLALASYLGNIKRNFSKAGVKIAAFSIILIPAL
                     IILAQNETGSALVFLSFILVLYREGLSAKVLIAGAIIIALFVLAL"
BASE COUNT          400 a          289 c          318 g          435 t
ORIGIN      
        1 ttcaaactgg cgccattact ccagaaagcc gattctattg caatgggaaa gccagtctac
       61 ccattgcctg ttcgcatagt cattactctc ctctcgaatt gataggggct atagaacaat
      121 cgtgtaatcc ttatttttgg ggtgttttca aaactaccat agaaaaaacg ggagggggtg
      181 atattcataa agggtataat atatggcggc aaaacgtgat gagtcttggg tttggtaatt
      241 atttcgacac cgatattccc aatcaggtta agggaaatat tccaagttct cagtattatg
      301 atcgttattt tggagaaaaa ggctggaaag ctttgactat acgttcgctc gccattggtc
      361 agggagagat tcttataact ccgctgcaac tggcaaatca ggcggtggta atcgccaaca
      421 aaggttatta ttatcctcct catgtagtaa aatctgttca gggtaaaccc acgcgctgga
      481 ataagaaaca aagcagtatc gattcacaac atttcgagac cattactaaa ggaatgtata
      541 gagtatttca aggagtatcg ggcacggggc gctggtataa gctggagaac atcgatattt
      601 gtggtaaaac gggtactgtt cagaatcccc atgggaaaga tcatagcctt tgtattgctt
      661 ttgctccagc cgaaaatcct aagattgcca tttccgtggt gatcgaaaat gctggttttg
      721 gtgcaacctg ggcagtacct attgcctctc tgatgataga gaaatatctc accggaaaga
      781 ttagtcgtac cgaattagaa gaaagaatga gccaagccaa tttattatga acactactcg
      841 atacaaatat cataatcctt ttgctgggat cgacagaaca ctcttgtggc tctatttcac
      901 cctggtcatc tttggatggt tgaatattta tgcagctgtt tacgacgaga ttcacaaaaa
      961 catatttgat ttgcagacca actatggcag gcaaatgatt tggatagcca cttctttcgt
     1021 tattgccatg gctattttgc taaccgatgt aaagttattc aattcattcg cgttttttat
     1081 ttatggattg gtaatcgtat cgcttattct cgtcttattc gttggcaaag agatttcagg
     1141 atcgaagtcg tggttgcaga taggccccat ttccattcag ccgggtgaat ttgccaaatt
     1201 tgctaccaat ctggccttag cctcctatct tgggaacata aaacggaatt tcagcaaggc
     1261 aggggttaaa attgcagcct tctcgattat tctcatcccg gctctcatta tattagcaca
     1321 gaacgaaacc ggatcggcac tcgtgttttt gagtttcatt ctcgtattat accgggaagg
     1381 actttcggcg aaggttctca ttgcgggagc cattataatt gctctgttcg tactggcact
     1441 cg
//
LOCUS       DAORPD010000534         1441 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_17775_length_1441_cov_1.779221, whole genome shotgun sequence.
ACCESSION   DAORPD010000534 DAORPD010000000
VERSION     DAORPD010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17775_length_1441_cov_1.779221"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..996)
                     /locus_tag="PK976_06525"
     CDS             complement(<1..996)
                     /locus_tag="PK976_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67577.1"
                     /translation="MYIQHRNDDFIALRKKFPQFSFDSFAIHHEKDKIDFAYSFSISD
                     RYFFEPKIIIPIKPFFKIKELNPEHLNILAFHVGMIELISYWKASCSPLIKIKPFQLN
                     ADQIKWWKNLYYNGLGEFFYRNNIQVDSEDFVEIQCESQKDISSISFNGNEGYIIPIG
                     GGKDSVVTLELLKKLHLPLVPFIINPREATTETARQAGFRDENVFTVYRTIHPLLLQL
                     NDQGFLNGHTPFSALLAFYTLLASYISGIPRIALSNESSANESTVAGTLINHQYSKSF
                     QFENDFRNYVHKYLDPGLNYFSFLRPLNELQIAGIFSRFPHYHSIFRSCNVGSKTN"
     gene            1093..>1441
                     /locus_tag="PK976_06530"
     CDS             1093..>1441
                     /locus_tag="PK976_06530"
                     /inference="COORDINATES: protein motif:HMM:NF014659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HRC67578.1"
                     /translation="MNKLDKILTIRYNEVKPEAGKLLIAEPFLGDAYFGRSVVLLAGH
                     GEEGSFGLILNKQVQVQIHEIVKGMPTFGAKVFLGGPVHSTQIFYLHTIGEKLDGSLE
                     VIPGLWWGGDTDQL"
BASE COUNT          440 a          250 c          331 g          420 t
ORIGIN      
        1 attggttttg ctacctacat tgcagcttct gaaaatggaa tgataatgtg gaaatcgcga
       61 aaatatacct gcaatctgca gttcattcaa tggtcggaga aaactgaagt aattcagtcc
      121 aggatcaagg tatttatgaa cgtagtttct gaaatcattt tcgaactgaa acgatttgga
      181 atattggtga ttgataagtg ttccagcaac cgtgctctcg ttggcgctcg attcgttcga
      241 aagcgctatc cggggaattc cactgatata tgatgccagc aatgtataaa aagccaataa
      301 agccgaaaaa ggggtatgcc cattcagaaa gccctggtcg ttgagttgaa ggagcaaagg
      361 atgtatggta cgatagacgg taaaaacatt ttcatcgcgg aatcctgcct gacgtgcagt
      421 ttcggttgtg gcctcgcgag gattgatgat aaaggggaca aggggtagat gcagtttttt
      481 caatagttcg agcgttacga ccgaatcttt accgcctcct atagggatga tataaccctc
      541 attgccattg aaactgatgg aagaaatatc tttctgactt tcacattgta tttctacaaa
      601 atcctctgaa tcaacctgta tattattgcg atagaaaaat tctcccaatc cattgtaata
      661 aagattcttc caccatttaa tctgatcggc atttaattga aaaggcttta tctttatcaa
      721 aggagaacag cttgctttcc aataacttat cagttcgatc attccgacat gaaaggcaag
      781 aatattgaga tgttcgggat tcaattcctt gattttgaaa aatggcttga tggggattat
      841 aattttgggt tcgaaaaaat accgatcgct aatgctgaaa gaatatgcaa agtcgatttt
      901 atccttttca tgatgaatgg cgaatgaatc gaacgaaaac tgggggaatt ttttacgaag
      961 ggctatgaaa tcatcattgc gatgttgaat atacataaat tttggtatat tttttgaaaa
     1021 tttaacagcg aatcaggcaa ccttttttaa aatgagcgtc caaagatata aagaaaagcc
     1081 catcacacta agatgaacaa gttggataag atactgacta taaggtacaa tgaggtgaag
     1141 cccgaggcag ggaagctatt gatagccgaa cctttcctgg gggatgccta ttttggcagg
     1201 tcggtagtct tattggccgg acatggcgaa gaaggttcgt ttggattgat tttgaacaag
     1261 caggttcagg ttcaaattca tgaaattgtg aaagggatgc ctacgtttgg agctaaagtt
     1321 tttttaggtg gccctgtcca ctccacacag atattttatc ttcataccat cggagagaaa
     1381 ctggatggga gcctggaagt cattccggga ctgtggtggg gaggtgatac cgaccaactc
     1441 t
//
LOCUS       DAORPD010000535         1438 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_17836_length_1438_cov_3.057122, whole genome shotgun sequence.
ACCESSION   DAORPD010000535 DAORPD010000000
VERSION     DAORPD010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1438
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17836_length_1438_cov_3.057122"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="PK976_06535"
     CDS             <1..492
                     /locus_tag="PK976_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67579.1"
                     /translation="LSTRLGHRYFDHKTEISLNNIKIGVSKEGMDFIPEKKVLPNVMK
                     MRGRSPILEVNNRYDTEKIILYRKIIHLDREKLDEVSPYSTLGINEILRLNVDSFMQK
                     MDDPAVPLFIPDENKGYAIINGERIYYINMIMQMTYENETTYRRYRIVLNRKGIKAIE
                     SFS"
     gene            complement(489..1187)
                     /locus_tag="PK976_06540"
     CDS             complement(489..1187)
                     /locus_tag="PK976_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018464587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRC67580.1"
                     /translation="MKKLKILLAEDDKNLGTVLKAYLDAKGHDTILAIDGEEALKLFY
                     AEDFDFCILDVMMPLKDGFAVTKQIRKSNKTIPILFLTAKSQPHDIITGFNTGADDYI
                     TKPFRMEELMARIKAIMRRTTSGLQDDLTHQVYHIGNYSFDFTSQILKIGDTEFNLTA
                     KEAELLKILVENANEVIDRSIILQNVWKDDSYFTARSMDVYISRLRKYLQEDRRVQII
                     NSHKVGFRLVVPED"
     gene            complement(1240..>1438)
                     /locus_tag="PK976_06545"
     CDS             complement(1240..>1438)
                     /locus_tag="PK976_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009235478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRC67581.1"
                     /translation="SKENQGMLFDKLYRVPTGNIHNVKGFGLGLSYTKAIVEAHQGNI
                     SVSSELKKGTTFTVTLPNFEP"
BASE COUNT          420 a          287 c          281 g          450 t
ORIGIN      
        1 ttgtctacgc gcctgggaca tcgctatttc gatcataaaa ccgaaatttc cctcaacaac
       61 ataaagattg gcgtaagtaa agaaggcatg gattttattc ccgagaaaaa ggttctgcct
      121 aacgtaatga agatgagagg tagatcacct attctggagg ttaacaaccg ttatgatacc
      181 gaaaaaatca ttctgtaccg taaaataatt cacttagacc gtgaaaaact cgatgaagta
      241 agcccttatt ctactttggg tatcaacgaa atattgcgat tgaatgtaga tagtttcatg
      301 cagaagatgg atgatcctgc cgtgcctctt tttatacccg acgaaaataa gggttatgct
      361 atcattaatg gcgagcgaat atattatatc aatatgatca tgcaaatgac ctatgaaaat
      421 gaaactacct accgcagata ccgtatcgta ttgaatcgca aagggatcaa agccatcgaa
      481 agtttttcct aatcttcggg aaccacgagc cgaaaaccca ctttatggct attaatgatt
      541 tgcacacgac ggtcctcctg taaatatttc ctcaatcgtg aaatgtatac atccatgctg
      601 cgagcagtaa aataagaatc atctttccag acattttgca agatgatgct tctatcgata
      661 acttcattgg cattttcgac caggattttc agcagttctg cttccttggc cgtaagattg
      721 aattcagtat ctccaatttt tagaatctga ctggtaaagt cgaaactata gttacctata
      781 tgatatactt ggtgggtcag gtcgtcctga aggccagaag tagttcttcg catgatggct
      841 tttatgcgtg ccatcagttc ttccatccgg aatggcttgg taatataatc gtccgctcct
      901 gtattgaaac ccgttataat gtcgtgtggt tgagatttgg ccgttaaaaa taaaataggg
      961 atggttttgt tggatttgcg gatttgtttt gtcacagcaa atccatcttt caaaggcatc
     1021 atcacgtcca atatgcagaa gtcgaaatcc tctgcataaa acaatttgag ggcttcttcg
     1081 ccatctatgg caaggatagt gtcatgaccc ttggcatcca gataggcctt caatacggtt
     1141 cctaaattct tgtcgtcttc ggccagcagt atctttaact ttttcatttt cggataaaat
     1201 tgacattttc aaagcaaaag tcttctaagg gtggtgtagt tatggttcaa aattgggcaa
     1261 ggttaccgta aaggtagttc cttttttcaa ttcactgcta actgaaatat ttccctgatg
     1321 ggcctccacg atggcttttg tataactcaa gcccaatcca aatcctttca cattgtgaat
     1381 atttcctgta ggaacacggt ataatttgtc gaaaagcatt ccttgatttt ctttacta
//
LOCUS       DAORPD010000536         1438 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_17842_length_1438_cov_2.347795, whole genome shotgun sequence.
ACCESSION   DAORPD010000536 DAORPD010000000
VERSION     DAORPD010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1438
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17842_length_1438_cov_2.347795"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /locus_tag="PK976_06550"
     CDS             <1..870
                     /locus_tag="PK976_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67582.1"
                     /translation="ALDGFQNIHRAQFFHKSREDEPAFIEKLLRHASLFQARKQSSQM
                     SLFGEFEEFALPKIELPACEPWDQLTRLHYEKEVTGFYISGHPLDLFKLEIDNLCTHS
                     IKDFKNDLEAHAGKEIIFAAMVSEAKERYGKNNKIYCSIVLEDFDETYSVTLFSEQYL
                     KYKHLLTPGSNILIRATAKQRFNGSSYELGINQIGLLSDALDNWVEKTQVFIPLPIMD
                     DHLIQQLDQSIRENPGTSTVSFTVFDPADQLSLNFENDNLLINTSGFARELKKIDGIF
                     FKFFKKKYWSDSL"
     gene            915..1232
                     /gene="trxA"
                     /locus_tag="PK976_06555"
     CDS             915..1232
                     /gene="trxA"
                     /locus_tag="PK976_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009291563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRC67583.1"
                     /translation="MAKILTDQNFEEVVLKSDKPVIVDLWAEWCGPCRNLSPIIEDLA
                     KEYAGKVVVGKLDVDNNPQTTIRLKVRNIPTILFFKNGEIVDKQVGTVPKSVLVNKIE
                     KLL"
BASE COUNT          465 a          297 c          277 g          399 t
ORIGIN      
        1 gcactcgatg gcttccaaaa cattcacaga gctcaattct ttcacaaaag ccgggaagat
       61 gaacccgctt tcatcgaaaa gctcttacga catgcttcct tgtttcaagc aagaaagcaa
      121 tcgagccaga tgagtttgtt tggggaattc gaagaatttg ctcttcctaa aatcgagcta
      181 ccggcatgcg aaccttggga tcagcttaca aggcttcatt acgaaaaaga agtaaccggt
      241 ttttatatct caggccatcc cctggattta ttcaaattgg aaatcgacaa tttatgcacc
      301 catagtatca aggattttaa aaatgacctc gaagctcacg ccggcaagga aatcattttc
      361 gcagctatgg tctcggaagc caaggagaga tatggaaaaa acaataaaat ttactgctcg
      421 atagttttgg aggattttga tgaaacatac tctgttacct tgttttcaga acaatatctc
      481 aaatacaagc acttattgac gccaggaagc aatattctga taagagctac agcaaagcaa
      541 cgtttcaatg gttcttccta cgaattggga ataaaccaaa ttggtttact ttcggatgcg
      601 ctcgataact gggtggaaaa aactcaagtt ttcattccct tgccaataat ggatgaccat
      661 ttaattcaac agcttgacca atcaattcga gaaaaccccg gtacctcaac cgtatctttc
      721 acggtattcg atccagcaga tcagctatcg ctaaatttcg aaaacgataa cttgcttatc
      781 aatacatcag gctttgccag agaattgaag aaaatcgacg gtattttttt caaatttttc
      841 aaaaaaaaat actggtctga ttcactctga gagaaagaat taattttgtc atccatcaac
      901 aaaaatttac aattatggct aaaattctta cagatcagaa ttttgaagaa gtggttctga
      961 aatcagataa acctgtaatt gtcgacttat gggccgaatg gtgcggtcca tgccgaaact
     1021 tgtcccccat catcgaagat ctggccaagg aatatgccgg aaaggttgtt gtcggaaaac
     1081 tcgacgtcga taataatcca caaaccacta tccgattaaa agtcagaaat atccctacaa
     1141 ttctattttt caagaacgga gaaatagtgg acaaacaagt tggaaccgtt cctaaatcgg
     1201 tcctggtcaa taagattgaa aagcttttat gattgttgtc cgaaaaattg ccgttacgaa
     1261 gcaaaaggat gtaataagta aggtctaccc acacgaagag cgaatatgcc agttatcgat
     1321 cctttacgtt ttcagccctt aaccgggctg gaattttttg cccgggaagt agttgaaggc
     1381 tttatcactg gccttcacaa aagtccttat cacggctttt cggttgaatt tgccgaac
//
LOCUS       DAORPD010000537         1438 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_17845_length_1438_cov_2.070860, whole genome shotgun sequence.
ACCESSION   DAORPD010000537 DAORPD010000000
VERSION     DAORPD010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1438
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17845_length_1438_cov_2.070860"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..785)
                     /locus_tag="PK976_06560"
     CDS             complement(117..785)
                     /locus_tag="PK976_06560"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HRC67584.1"
                     /translation="MDEITDKEKLLKNIRNALIDKVENPFHDLDLDSSVYPPIEESPD
                     IYFVRRFIEVGGKFVYCANELELSENLSDLLVNENLFPVFTPDERIRYLLLQNGIDVI
                     HNEDDFYEMKTGITSCEYLVARTGSIVFSSRLSSGRRMITVPEVHIVIAHTSQIVVEI
                     KEALANIRLKYQKQLPSFITFVTGPSRTSDIEKTLVMGAHGPAKLYLFLIEEEIKPIF
                     ENLL"
     gene            complement(790..>1438)
                     /locus_tag="PK976_06565"
     CDS             complement(790..>1438)
                     /locus_tag="PK976_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HRC67585.1"
                     /translation="KVTIVPRGKALGAAWYLPEERQITTFEQMFDEITATLGGRASEK
                     INFGEISTGALNDLERVTKQAYNMVAYFGLNERLGNISYYDSTGQQEFAFVKPYSEET
                     ARIIDEEIKKLVDTAYQRALKILQDNKEKVDKLATRLLEKEVIFREDVEDIFGPRPEG
                     SMDIVIEPEKSVKKERETQPAESPMETKNIEEAPISNRTDIDTNNSSKSSNFKDR"
BASE COUNT          373 a          293 c          292 g          480 t
ORIGIN      
        1 tggcttctat tccattttct tctaagagtc ctttgaccag aagagcctga taattacggc
       61 tagccgtgta aaccagagca aattcatttt cttccatgca acaattattt tagattctat
      121 aacaaatttt caaagattgg ttttatttcc tcttcgataa ggaagagata aagtttagca
      181 ggcccatggg ctcccatgac gagggttttt tctatgtcgg atgtgcggct tggtccggta
      241 acaaaggtga taaaagaagg gagctgcttt tgatatttga gtcttatatt tgcaagggct
      301 tcctttattt cgacaactat ttgcgaagta tgggcaataa cgatatgcac ttctggtact
      361 gttatcattc ttctgcccga actcaacctc gatgaaaata ctatcgaacc tgtccgagcc
      421 acgagatatt cgcatgaagt aatacccgtt ttcatctcgt agaagtcatc ttcattgtga
      481 ataacatcaa ttccgttttg aagcaggaga taacggattc tttcatcggg cgtaaaaacg
      541 ggaaacaaat tttcgttgac caataaatcg gacaggtttt cggagagttc cagttcattg
      601 gcacaataga cgaattttcc cccaacttcg atgaaacggc gaacaaaata aatatcggga
      661 gattcttcaa tgggcggata taccgaagaa tccaagtcga ggtcatgaaa aggattttcc
      721 accttatcga tcagagcatt tctgatattt ttcaggagtt tttccttgtc tgtaatttcg
      781 tccatgaatt catcgatctt tgaagttgga agatttgcta ctgttgtttg tatcgatgtc
      841 agttcgattc gaaatgggag cctcttctat atttttcgtt tccatggggc tttcggctgg
      901 ctgggtttct ctttcttttt ttaccgattt ttcgggttca ataacaatat ccatgctacc
      961 ttcagggcga ggtccaaaga tatcttcgac atcttccctg aaaataactt ctttttcgag
     1021 aagacgggta gcaagtttat ctactttttc cttgttatcc tgaagaattt ttagagctcg
     1081 ttgataagcc gtatctacga gttttttaat ttcttcgtcg ataattcgag ccgtttcttc
     1141 actatagggt tttacgaaag caaattcttg ctgacctgta gaatcgtaat aagatatatt
     1201 tccgaggcgt tcgttcaggc caaaataagc caccatattg taagcctgtt ttgtaactct
     1261 ttcgaggtcg ttaagtgcgc cggtactaat ttcgccgaag tttatctttt ctgaagctct
     1321 accgcccaga gttgcagtaa tctcgtcgaa catttgttcg aaggtagtaa tctgcctttc
     1381 ttcgggcaga taccatgctg cacccagagc ctttccacga ggaactatgg ttaccttc
//
LOCUS       DAORPD010000538         1438 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_17850_length_1438_cov_1.820680, whole genome shotgun sequence.
ACCESSION   DAORPD010000538 DAORPD010000000
VERSION     DAORPD010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1438
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17850_length_1438_cov_1.820680"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /locus_tag="PK976_06570"
     CDS             complement(<1..748)
                     /locus_tag="PK976_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HRC67586.1"
                     /translation="MNENSSVLIIYTGGTIGMMHNPITGALEPLDFNHLYKHLPVLEN
                     LKCRIDTYSFDPVIDSSDMNPDFWIELAEVIEKNYESYDGFVVLHGTDTMAYSASVLS
                     FMLENLNKPVVFTGSQLPIGMIRSDGRENFITAVEIATAYKDGTPIVPEVSICFENKL
                     FRGNRTTKFNAEHFDAFLSGNYPPLAVVGVHIHFNENDILKPNFKKLKVHKKLDTHIM
                     ILKLFPGIHPQKVEEFLHIDGLKGVIMETFG"
     gene            complement(753..>1438)
                     /locus_tag="PK976_06575"
     CDS             complement(753..>1438)
                     /locus_tag="PK976_06575"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRC67587.1"
                     /translation="AIDSTTHKHLLSLTNSYPEHFYPMMGLHPTSVKENYMDELAIVH
                     HYLTNHSFVAIGEVGIDLYWDMTFEKQQVEAFKIQAQWAREMGLPIVVHSRNSTEVIL
                     QVLEEIGYKGLSGVFHCFNGTLEQALEIASKGFYLGIGGVITYKNSSLSSIVARVPRD
                     FVVVETDAPYLPPVPHRGKRNQPAWIKYTVNKLAEIWQISPEEAAGITTANAIRLFQK
                     NCDLSKLKL"
BASE COUNT          436 a          292 c          295 g          415 t
ORIGIN      
        1 ttccaaaggt ttccatgatg actcccttca atccatcgat atgaagaaac tcttccacct
       61 tttggggatg aatccctgga aaaagtttca ggatcataat atgggtatca agcttcttgt
      121 gaaccttcag tttcttaaaa ttcggtttga gaatatcatt ctcattgaaa tgaatatgga
      181 cgcctaccac agctaatggg ggatagtttc ccgataaaaa ggcatcaaaa tgttcagcat
      241 tgaatttagt cgttcgatta cccctgaaaa gtttgttctc gaaacaaata cttacttctg
      301 gtacaatagg agtaccatct ttataggctg tagcaatttc gacagccgta atgaaatttt
      361 cacgcccgtc ggaacgtatc atgccaatgg gcagctgaga tccagtaaat actacgggtt
      421 tattcaaatt ttcaagcatg aagctcaaaa ctgaggcaga gtaggccata gtatccgtac
      481 catgtaagac gacgaatcca tcataacttt cataattctt ttcgatgact tcggccagtt
      541 cgatccagaa atcgggattc atgtcggatg aatcaatgac tggatcgaaa gaataggtat
      601 caattctaca tttgaggttc tctaatacag gcaaatgctt atataaatgg ttgaaatcga
      661 gaggctcgag agctcctgta ataggattat gcatcatacc aatggttcca cctgtataaa
      721 tgatcaacac ggaactattt tcgttcataa ggttataatt ttaatttcga caaatcacaa
      781 tttttttgga aaagccggat tgcatttgca gtggtaatac cggcagcttc ttcgggcgag
      841 atttgccaga tttcggcaag tttgttcaca gtgtacttta tccatgcagg ttgattgcgt
      901 ttaccacggt gaggtacagg aggtaaatat ggtgcatcgg tctccacaac aacgaaatca
      961 cgtggcactc gggcaacgat agaggagagg gatgaattct tataggtaat gacacctcca
     1021 atacccagat aaaaaccttt tgaagcgatt tcaagagctt gttcaagagt accgttaaaa
     1081 caatggaaaa ctccactaag gcctttgtaa ccaatctctt cgagcacctg cagtatcact
     1141 tctgtcgaat ttcgtgaatg aacaacgata ggcaaaccca tctctcttgc ccattgcgct
     1201 tgaatcttga aagcttccac ttgctgcttc tcgaaggtca tgtcccagta taaatcgatt
     1261 cctacctctc caatggccac aaatgaatga tttgttaaat aatgatgaac aatggcaagt
     1321 tcatccatgt aattttcctt caccgaggtt ggatgcaaac ccatcatagg ataaaaatgc
     1381 tcgggataag aattcgtaag agatagaaga tgcttatggg tagtgctgtc gatggctg
//
LOCUS       DAORPD010000539         1436 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_17872_length_1436_cov_2.671977, whole genome shotgun sequence.
ACCESSION   DAORPD010000539 DAORPD010000000
VERSION     DAORPD010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17872_length_1436_cov_2.671977"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="PK976_06580"
     CDS             <1..236
                     /locus_tag="PK976_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67588.1"
                     /translation="KLNPKCGFVLMDKLEQMDLDTLSEFGQWLEQEGLQAIATRVSTG
                     PECSILIQDGYVVGAEQPVQETQEAPKWKAGEF"
     gene            240..794
                     /locus_tag="PK976_06585"
     CDS             240..794
                     /locus_tag="PK976_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67589.1"
                     /translation="MPPLVDLTGKRFGRLVVVKRNGSQHGHALWLCVCDCGNKSVASS
                     NDLRRGNTKSCGCLRNEITATRSKAAGIVRGKQLRKHGKAGTRLYNIWKSMRDRCNNP
                     QNRDYKDYGGRGISICKEWDNYESFYQWAMNNGYDPNATFGDCTIDRINVNGNYEPVN
                     CRWVNLKAQANNRRPRKEKDKWNS"
     gene            782..>1436
                     /locus_tag="PK976_06590"
     CDS             782..>1436
                     /locus_tag="PK976_06590"
                     /inference="COORDINATES: protein motif:HMM:NF024870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRC67590.1"
                     /translation="MELVKGPIQRAQKICLYGVEGIGKSYFASKFPNPIFIDTEDGTS
                     HMDVIRTPKPSSWTMLMSQVEYFRFHPSELDTLVIDTLDWAEQLCVEHICSKNKKDGL
                     ESFGYGRGYTYLAEEFGRLLNSLSDLVDLGINVMVVAHATMRKFEQPDELGAYDRWEM
                     KLQKKTAPMVKEWADIVLFANYKTYVVNVDGQGAEKGTNKARGGKRVMYTTHHPCWDA
                     "
BASE COUNT          460 a          272 c          363 g          341 t
ORIGIN      
        1 ggaaactcaa tccaaaatgc ggctttgtgt tgatggataa attagagcaa atggatctgg
       61 ataccttatc tgaattcggc cagtggttgg agcaggaagg gctgcaggct attgctacgc
      121 gggtcagcac tggcccggaa tgttcaattt taattcagga tgggtatgtc gtaggtgctg
      181 aacagccggt gcaggagaca caggaagccc cgaagtggaa ggcaggtgag ttttagaaaa
      241 tgcctcctct tgtagatttg acaggtaagc gttttggcag attagtagtt gtcaaacgta
      301 acggatcaca gcatggacat gcgttatggt tgtgcgtttg tgactgtggt aataaatcag
      361 tagcttcatc gaatgatttg cgccgcggta atacaaaatc atgtggttgc ttaagaaatg
      421 aaattacagc tactcgaagt aaggctgctg gcatcgttcg agggaaacaa ttgcgaaaac
      481 atggcaaggc tggaacaaga ctatataaca tctggaaatc tatgcgagat cgatgtaata
      541 acccccaaaa cagagattat aaagattatg gtggccgagg aatcagtatt tgtaaagaat
      601 gggacaatta cgagagtttc tatcaatggg ctatgaataa tggctatgac ccaaacgcta
      661 cctttggtga ttgcactatt gatcgcatca atgtaaacgg taactatgaa cccgtaaatt
      721 gtagatgggt caacttaaaa gctcaagcta ataaccgaag accaagaaag gagaaggata
      781 aatggaactc gtaaaagggc caattcaaag agctcaaaaa atatgcctat acggtgtgga
      841 aggaatagga aaatcatact tcgcttcaaa gtttccgaat cctattttta tcgataccga
      901 agatggcact tctcatatgg atgtaatcag aactcctaaa ccaagcagct ggacaatgtt
      961 aatgagccaa gtagagtatt tccggtttca tccaagtgaa ctagataccc tggtaatcga
     1021 tactttggat tgggcagaac agttatgcgt agagcatatt tgctcaaaaa acaaaaaaga
     1081 cggactggaa tcgttcggtt acggaagagg ctacacctac ctggcggaag agtttggccg
     1141 gctgttaaat agcttatcag acctggtgga tctgggaatc aatgtcatgg tcgtggccca
     1201 tgccaccatg cgaaagttcg agcagccgga cgaattaggc gcgtacgacc gctgggaaat
     1261 gaaactacaa aagaaaacag ctccaatggt aaaggaatgg gctgacatag tattattcgc
     1321 caattacaaa acctacgtag tcaatgtaga tggccaggga gcagaaaaag gtaccaacaa
     1381 agcccgtggc ggcaaacgcg ttatgtatac cacccatcac ccctgctggg acgcaa
//
LOCUS       DAORPD010000540         1433 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_17934_length_1433_cov_2.500726, whole genome shotgun sequence.
ACCESSION   DAORPD010000540 DAORPD010000000
VERSION     DAORPD010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17934_length_1433_cov_2.500726"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1023)
                     /locus_tag="PK976_06595"
     CDS             complement(<1..1023)
                     /locus_tag="PK976_06595"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HRC67591.1"
                     /translation="MKKIHILVVKSYLGPLVLTFFIALFVLLMQFLWKYVDDLVGKGL
                     EWFHIANLMVYASTTFVPLALPLAILLSSIMTFGNLGEHYELVAMKSAGISLGKAMRP
                     LIVLSVLISIGAFLFSNMVLPVANLKFKSLLYDIRKQKLALNIQPGLFYNGIDNYTIR
                     VDQKNDETNTVYGVIIYDHSAQLGNMKFTTASEGKMQLTPDEKFLIFTLHNGYNYEEL
                     FNEKDYRNRRQFQIVRFDENRQKFDMTGFGLQRTDEELFRKNYQMMNVSQLNYAIDSL
                     EKEYRIRVKEVSRSIYSRYSILQNADTLHKETSMRFSLGQLDASERERVITIALQNTR
                     NLKEYLS"
BASE COUNT          429 a          274 c          292 g          438 t
ORIGIN      
        1 cgataaatat tccttcaggt ttctcgtgtt ttgaagagca attgtgataa ccctttccct
       61 ttcgctagca tcgagctgcc ccagagaaaa tctcatggaa gtttctttgt gcaatgtatc
      121 tgcattttgc aaaatggaat atcgcgaata gatggaacgt gagacttcct ttacccgtat
      181 tctgtattct ttctcgagtg agtctatggc ataattcaac tggctcacat tcatcatctg
      241 gtagttttta cgaaataatt cctcatctgt tcgttgaagc ccaaacccgg tcatatcgaa
      301 tttctgccta ttctcatcga atcgtacgat ttggaactgc ctgcgattgc gataatcttt
      361 ctcgttgaac aattcttcat agttataacc attatgaagt gtaaaaataa ggaatttttc
      421 gtcaggggta agctgcattt taccttcact ggctgtggta aacttcatgt tgcccaactg
      481 agcactatga tcgtaaatga ttacgccgta aacggtgttg gtttcatcgt tcttttgatc
      541 gaccctgatg gtgtaattgt caattccgtt gtagaataag cccggctgta tattcaatgc
      601 cagcttttgt ttacggatat catacagtaa agatttgaat ttcaggttgg ccaccggaag
      661 aaccatattg ctgaataaaa aggctccaat gcttatcaat accgatagaa cgataagtgg
      721 acgcatggct ttgcccagac tgatgccggc cgatttcatg gccacgagtt catagtgttc
      781 gccaagattt ccgaaagtca tgattgaaga aagcaggata gccagaggta aagcaagagg
      841 aacgaaggtt gtagaagcgt aaaccattag attggcaata tggaaccatt cgagcccttt
      901 acctaccaga tcgtcgacgt atttccataa aaattgcatc aaaaggacga aaagtgcgat
      961 gaaaaatgta agcaccaatg gccctaaata cgatttaacc actaaaatgt gaatcttttt
     1021 caagacgctt gaattaactg tgcaaaatta ataagaaaca ctccattcgg cgatttcgta
     1081 tcgcaaaccc gtttcacaca tcgatgtgta aatatctgac attaaaaata tttgaaattt
     1141 taaacgggtt ttcaattatt tatcatgttg ggttttcagc tgaattgatt ttgaaggcat
     1201 aaatagtaaa aaatttaatt ttgccgaaat cgtaactcct atggaattga ttcgagtatc
     1261 agataagcga acctcacgga tgtttcttga tacggccaga ataatttata ggaatgatcc
     1321 tgtgtgggtt tgtccactcg atcagaccgt tgaagatatt ttcgatccaa ggcaaaatgt
     1381 atttttttcg catggagaag ccgaacgctg ggtgctgaaa gaaaaaggga cat
//
LOCUS       DAORPD010000541         1430 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_18002_length_1430_cov_2.226182, whole genome shotgun sequence.
ACCESSION   DAORPD010000541 DAORPD010000000
VERSION     DAORPD010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1430
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18002_length_1430_cov_2.226182"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1176)
                     /locus_tag="PK976_06600"
     CDS             complement(<1..1176)
                     /locus_tag="PK976_06600"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HRC67592.1"
                     /translation="MGFTNIFMDLQQDFMKNHISKFILISVISLLSYSCLGQTSISGK
                     IEGAAGMKLGIAYYYGENLRAFDSLRCNENGEVNLVVPKYIPPSQMCILTQQNDGFQF
                     FYDGKSLTFHTNISALQDSLNFIDSKGNTDFLKFTKYEVRYEKIKELLENMLDEYPHE
                     LPFYQEIINEYNLRQIDYEATLNKIITENQENLAGKYLMTRRKPFVSPNLLGEERKNF
                     LKQHFFDKISFDDKGLLYTNAYPRLVIEYLELYANPNFDKKKLEISLIEGIKIIMSIL
                     QPYAEAYDMITEYLLDGFQNFGFERVVQFISERYEQGNCINTNPHLTSRIEISKRLSP
                     GSPAPEIKGKDLRDHEIKLSNYKGNYVLLIFWGSWCNHCSALLPKIASLYARPHQKKW
                     "
BASE COUNT          439 a          242 c          274 g          475 t
ORIGIN      
        1 ccactttttt tgatgaggtc gagcataaag agaggctatt ttgggtaaaa gtgccgagca
       61 atggttacac cagctccccc agaatatcaa caatacatag ttacctttat agttgctgag
      121 ttttatttca tgatccctca aatcttttcc cttgatttcg ggggcaggcg agcctggtga
      181 aagacgcttc gaaatctcta ttcgggaggt aagatgggga ttggtattta tacaatttcc
      241 ctgttcgtaa cgttcggaaa tgaattgcac cacacgctcg aaaccgaagt tctgaaagcc
      301 atcgagcaaa tattctgtaa tcatatcata cgcttctgca taaggctgaa gtatactcat
      361 gattattttt attccttcta tcaatgagat ttctaatttc tttttatcga agttgggatt
      421 tgcatataat tccaaatatt ctatcacaag ccttggataa gcattggtat agagaagacc
      481 cttgtcgtcg aaagaaattt tatcgaaaaa atgttgtttc agaaaatttt tcctttcttc
      541 acctaagaga ttggggctaa cgaaaggttt tcgtcgagtc atcaaatatt taccagcgag
      601 attttcctgg ttctcggtaa tgattttgtt taaggttgcc tcgtagtcga tttgccgtaa
      661 attgtattca ttgataattt cttgatagaa aggaagttca tgaggatact catccaacat
      721 gttttccaag agttccttga ttttctcgta tctgacttcg tatttggtaa attttaggaa
      781 gtcggtattt cccttcgaat ctataaaatt caaactgtcc tgcaaggccg atatattcgt
      841 gtgaaaggta aggcttttgc catcgtaaaa aaattgaaaa ccatcgtttt gctgagtaag
      901 tatgcacatc tgggaaggag gaatatattt gggaacaacc agattaactt ctccattttc
      961 gttgcacctg agcgaatcga atgctcttag attttcacca taataataag caattcccaa
     1021 tttcataccg gcagcacctt ctatctttcc gctaattgat gtttggccta agcatgagta
     1081 ggagaggaga gatattacag aaatcaagat gaatttcgat atatggtttt tcataaaatc
     1141 ttgttgcaaa tccataaaaa tgttcgtaaa ccccacaatt aacaaattta tgccgtttta
     1201 gtcaatttat tatccaattt actacactta ttcatatttt atgaagtgaa taaacatata
     1261 atagaaagtg aatcaataag tagaatatat gaatgatgag ttttggtaat tctctatttg
     1321 gagtttaggg cgaagatgag ataagcggtt atctttgcac aaactgccaa aatatggacg
     1381 aaagcgaagt attgattttg agaaacaaga ttgagaattt gactgaggat
//
LOCUS       DAORPD010000542         1429 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_18018_length_1429_cov_2.538574, whole genome shotgun sequence.
ACCESSION   DAORPD010000542 DAORPD010000000
VERSION     DAORPD010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18018_length_1429_cov_2.538574"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1387
                     /locus_tag="PK976_06605"
     CDS             <1..1387
                     /locus_tag="PK976_06605"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRC67593.1"
                     /translation="LCRELYEKQNSHIYLKKSYLFYTLLVIIAMVSVWLANHQHNFAA
                     NFNGFTVVFCMLFLDFILWLHFFGVIRKYSKKKKCILCVLFWLPFMLFVFGAVYSFLQ
                     PFGLWPPTLQTWYLGSIFIVYSAKVLPVFIYVISDIIHWITGLKKLGKQKEKAFLDAL
                     LDMISSPLWVKFIARSGFSVVFIVLMFGAIVWPYRVEIKKLNFPLKDLPASFNGFRIV
                     QISDLHLGSWPSKKSLKKIVSKTNDLNPDLIVFTGDLVTFTTKEAYPFREILGKLHSE
                     EGVITILGNHDYGGYVKWASPNAKNKNLEDLFRLYQEMGWKLLLNESLVINKNGEQIA
                     ILGVENWGSQKRFPQRANLEKTLAQVENIPVKILLSHDPTYWEKIVNQRDISILLTLS
                     GHTHGMQIGIETGNFRWSPAKFIYTYWSGLYKDSKTPQYLYVNQGLGCIGYPGRMGIW
                     PEITLIELKVK"
BASE COUNT          420 a          243 c          286 g          480 t
ORIGIN      
        1 tttgtgccgg gagttatatg aaaaacaaaa ttctcatatc tatttgaaaa aatcatattt
       61 attttatact cttttggtta taattgccat ggtgagtgta tggctggcaa accatcaaca
      121 taatttcgca gctaatttca atggatttac ggtagttttc tgcatgctgt ttctcgattt
      181 tatcctctgg ttgcattttt tcggtgtcat tcgaaaatat agcaagaaga agaaatgtat
      241 cctgtgcgtt cttttctggc ttccttttat gctgttcgtt ttcggcgcag tttattcatt
      301 cctgcaacct ttcgggttat ggccgccaac gctgcaaaca tggtatctag gatcgatttt
      361 cattgtttat agtgcaaaag tattgccggt tttcatttat gtaatttctg acatcataca
      421 ctggattacc ggattaaaaa agttaggcaa acaaaaggag aaagcttttt tagatgcttt
      481 gctcgatatg atttcatcgc ccttatgggt aaagtttatt gcacgatccg gtttttcggt
      541 tgtttttatc gttttgatgt ttggggccat cgtctggcca tatcgagtcg aaataaaaaa
      601 actgaatttt ccccttaaag atttacctgc ctcattcaat ggatttcgca tcgtacagat
      661 ttcggacctg catctgggca gttggccttc aaagaaatca ttgaaaaaaa tagtaagcaa
      721 gacgaatgac ttgaatcctg atctcatcgt atttacgggt gatctggtta ccttcacaac
      781 taaagaagcc tatcctttcc gtgaaatttt gggaaaactc cattcagaag aaggcgtaat
      841 aaccatttta ggcaatcatg attacggtgg atatgtgaaa tgggcttcac ctaatgcaaa
      901 gaataaaaat ctggaagatt tattccgttt atatcaagaa atgggctgga aactgcttct
      961 taatgaatct ttggtcatta ataagaatgg ggaacagatt gcaattttgg gagtagaaaa
     1021 ctggggcagt cagaaaagat ttccccaaag agccaacctt gagaagactt tggctcaagt
     1081 tgaaaatatt cctgttaaga ttcttctttc acacgatcca acttattggg aaaaaatagt
     1141 gaatcagaga gatatatcta ttcttcttac gctttccgga catactcatg gaatgcaaat
     1201 tggtatcgaa accggaaatt tccgctggag ccctgcaaaa tttatttaca catattggtc
     1261 gggtttatat aaagatagta aaaccccgca atatctctat gttaatcaag gtttgggatg
     1321 tattggttat cccgggagga tggggatatg gcctgaaatc actttaattg aattgaaagt
     1381 taagtaagca tttaaatatc taatttattt tagccatttt gagtagtgt
//
LOCUS       DAORPD010000543         1428 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_18044_length_1428_cov_2.720320, whole genome shotgun sequence.
ACCESSION   DAORPD010000543 DAORPD010000000
VERSION     DAORPD010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18044_length_1428_cov_2.720320"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /locus_tag="PK976_06610"
     CDS             <1..779
                     /locus_tag="PK976_06610"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRC67594.1"
                     /translation="PCGMVKDSLKLTINPKPELVLTPPSPALCRDSVLILSASGSETF
                     KWTPNQDIDTTYGSLVKISPKHDMDYSLIGMNHSGCRDTLTIHVTVKPAPYLNLGEDL
                     YLFSCDPIILDAGGGDGSDFYEWQDGSTKRTLKVYGNGTYWVRVFNEGCAVVDTIKVQ
                     LCEGFIYVPYAFTPNNDGLNDMFKVITTDPTIHFHIYIYNRHGQLVFQTDDIEKGWDG
                     KIDGEMAPADVYVYLIEYRGNGTHSPGVTKTLKGSFILVR"
     gene            complement(1259..1330)
                     /locus_tag="PK976_06615"
     tRNA            complement(1259..1330)
                     /locus_tag="PK976_06615"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1295..1297),aa:Arg,seq:cct)
BASE COUNT          459 a          267 c          262 g          440 t
ORIGIN      
        1 atccatgtgg aatggtaaag gacagtttga aacttaccat caatcccaaa cctgaactgg
       61 ttctgactcc gccttcacca gccctttgcc gagattctgt actgattttg agtgcttcgg
      121 ggtccgagac gttcaaatgg acgccaaacc aggacattga tacaacttat ggctctcttg
      181 tcaagatttc accgaagcat gatatggatt attctctgat tgggatgaat cattcaggct
      241 gtcgtgatac actcactatt catgttaccg taaaacctgc gccatatctg aatctgggag
      301 aagacctcta tctattctca tgcgatccta ttattttgga tgcaggcgga ggagatggga
      361 gtgattttta tgaatggcag gacggttcta ctaagcgcac cttaaaagtg tatggaaatg
      421 gaacatattg ggtacgtgta ttcaatgaag gctgtgcagt tgtagacacc ataaaagtac
      481 agctctgtga gggttttatt tatgtgccct acgcctttac accaaataat gacggtttga
      541 acgatatgtt taaggtaatt accaccgatc ctacgattca tttccacatt tatatctata
      601 atcgccatgg tcaattggta tttcagacag atgatatcga aaaaggttgg gatggtaaaa
      661 ttgatggaga aatggctcca gccgacgttt acgtttatct gatcgaatat cgtggcaatg
      721 ggacacattc gccaggagtg accaaaaccc taaaagggag cttcattctt gtaagataga
      781 aactttgatg tctcttctcc ccgtattgct aatgatgttt tcgttattat agattcttga
      841 ggctttcaat ctattatcca atttttaatt gctaattgtt aataaaaatt tcaacattct
      901 caacaagctc acgaaaataa caatgatggc tattcctcct ctctaaggag aacaagtaaa
      961 catgcctact ttaaaaatac tacaaaaagt ttgaatccaa caaagaaaac attaaggaaa
     1021 taaatttcga taaaaacttc tatcgacgag ttgcttctca aagacttttt caaaaatatt
     1081 attccttatc tgtaattttt tgaatatcta aaaaataatt ttcacctgag aataaaatat
     1141 aatacaacct ttcccttttc ggcaaataaa agaaagaatt ttttggaaaa tttaatcttc
     1201 agcgaaaaac tctatttatt aataaatata tggaaggtta aggagttgac gatgtttgta
     1261 gccccaccag gaatcgaacc tggatttaaa gtttaggaaa cttccgttct atccgttgaa
     1321 ctatggggcc caaagtgggc ggcaaagata cagagttttt aacaaatctt atatcaatta
     1381 cccaagtaaa tatacttatg ataaaacaaa tcaactcgac tttgcctc
//
LOCUS       DAORPD010000544         1428 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_18046_length_1428_cov_2.474144, whole genome shotgun sequence.
ACCESSION   DAORPD010000544 DAORPD010000000
VERSION     DAORPD010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18046_length_1428_cov_2.474144"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            765..>1428
                     /locus_tag="PK976_06620"
     CDS             765..>1428
                     /locus_tag="PK976_06620"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HRC67595.1"
                     /translation="MRSKSFILLNVLIVVAALCHFTFAQEPLQCTSIVLTHWMTPDEF
                     LRRSEIGRGFTPTPPPPAPVTNLAEFNYNEGVLIRYPFGIPMALIKKMSEVGIVITIV
                     ANQSAQNTVYNQYVNNGVNLTNCSFLIAPTDSYWTRDYGPWFVINGENKVGIVDFPYN
                     RPRPSDDDIPVKMASFMDVPLYGMNIIHTGGNYMCDGMEAAASTNLVYSENPSLSPSQ
                     IHQ"
BASE COUNT          398 a          278 c          229 g          523 t
ORIGIN      
        1 gaagtatgta tagtatcatg aacgagtact ttctgagatt gtccttctaa tgtactaagt
       61 aaaataaata gaatgagtat tttcctgatt cctttcattt cttttattga gttacgtaaa
      121 gcccttttca agatatgaat gacgatatct ttgcaaaaat aatctataag ctcaaaattt
      181 tatcaatttt tgataagttt gtcaaatcgg gatcgaattc ttttcgattt aatagaccct
      241 catcgtattg agccgtttaa tttttctgga ttcattccat gccttttatt tttaatctat
      301 tgtaatataa tactttataa tcttctacca catgctttgc tatcagaatc ctgaaaatta
      361 atcacttaca atctcttaac cccaccccat tcatcattcc ttacaatgaa tcttagttgc
      421 ttaacaatgt caatcgagca ttttcagatc ttacaaagca tttatgattt gcctggcaac
      481 aatacccatg ataattccgc tcgactcagg agtgtatctc tttatttcct gtgtatgggt
      541 tgcttttcac tttcttagcg gtcatatcgt cccatttatg ctaatgatct tcaatcttat
      601 aaagaacaat ctgccactta tccattggga tttccgaaag aaattgctgg ataacctcat
      661 tttttatcta tctttacctg ctttttttct tttgtttttc tttaaagatg atccctggta
      721 tctctttcaa ttaacatcat tcgtaatcta taaaataggt ttttatgcgc tctaagagtt
      781 ttattcttct aaacgtttta atagtagtcg ctgccttgtg tcatttcacc tttgcacagg
      841 agcctttaca atgtacttcc atagttttaa cgcattggat gacacccgat gaattcttgc
      901 gtcgaagtga gattggtcga ggatttactc ctacccctcc cccacctgct ccagttacta
      961 atctggctga gtttaactac aatgaaggcg ttttaatacg ctatcctttt ggaataccta
     1021 tggcattaat aaaaaaaatg tctgaagttg gtattgttat taccattgtt gctaatcaat
     1081 cagcgcaaaa tacggtttat aaccagtatg ttaataatgg ggttaacttg actaattgca
     1141 gttttttgat tgctccaact gattcttatt ggacgagaga ttatgggccc tggtttgtaa
     1201 tcaacggaga aaacaaagtg ggtattgttg attttcctta caatcgtcca cgaccatcgg
     1261 atgatgatat accggtcaaa atggcaagtt ttatggatgt tcccctctat ggaatgaata
     1321 tcattcatac gggtggaaat tacatgtgcg acggaatgga agctgctgcc tctaccaatc
     1381 tggtatattc agagaatcct tccctaagtc cttcgcagat tcatcaaa
//
LOCUS       DAORPD010000545         1427 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_18067_length_1427_cov_2.184402, whole genome shotgun sequence.
ACCESSION   DAORPD010000545 DAORPD010000000
VERSION     DAORPD010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1427
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18067_length_1427_cov_2.184402"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="PK976_06625"
     CDS             complement(<1..568)
                     /locus_tag="PK976_06625"
                     /inference="COORDINATES: protein motif:HMM:NF017396.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S28 family serine protease"
                     /protein_id="HRC67596.1"
                     /translation="MLHRLILSITLPILMVLMLSYKPKPLSPEEVLSSIPLVDSFRVI
                     LSDKLFSETYEVWFRMPLDHSNPNGLSFPLRVYYSHNGFDKPMVVVVDGYTMYTSKAN
                     EVTCIVDGNQITIEHRFFSKSRPMDSIPWSYLNIRQAAADAHLVISAFKPFYHGKWIS
                     TGISKSGQATIYHRRFYPTDVNVSIPYVA"
     gene            complement(841..982)
                     /locus_tag="PK976_06630"
                     /pseudo
     CDS             complement(841..982)
                     /locus_tag="PK976_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018110133.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1099..>1427)
                     /locus_tag="PK976_06635"
     CDS             complement(1099..>1427)
                     /locus_tag="PK976_06635"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRC67597.1"
                     /translation="ETKIRLCSSDSVINSVVSKPSSKSNITREDISQASLGQYYLIFG
                     SYPNFQLARQAQIDLINDGFKNAFIFEKEGKFRTALDKYETMEAARAAKKQLPNKYKN
                     AWILKE"
BASE COUNT          408 a          273 c          301 g          445 t
ORIGIN      
        1 gggccacgta gggtatacta acgttaacat cggtgggata gaaacgccgg tgataaattg
       61 tggcctgccc gctcttgcta atacccgttg aaatccactt cccatggtag aaaggtttaa
      121 atgcagatat taccaaatgc gcatcagccg ctgcctgcct aatgttgagg tagctccatg
      181 gaatggagtc cattggcctg ctcttcgaaa aaaaacgatg ctcgatggtt atctggtttc
      241 cgtcaacaat gcaggttacc tcgttagcct tggaggtgta catggtatag ccgtcaacaa
      301 cgacaaccat tggtttgtca aaaccattat gagaatagta aacccgtaag ggaaatgata
      361 gaccattggg attggagtgg tcaagaggca ttctaaacca cacctcgtag gtttccgaaa
      421 atagtttgtc ggataggatt accctgaaac tgtctaccag cggtatggac gaaagaactt
      481 cttccggcga taagggttta ggtttgtacg aaagcataag caccatcaaa atcggcaggg
      541 taatgcttaa aattagcctg tgtaacatct tcaagcactt ttgttgtcgt tgttttcgtt
      601 tcatattatt catgcctaaa ttcaagtaat taatgcaact ttttggttag acgaattacg
      661 taataaaaat tcattcgaag atacaaagtt gagttttttt ggaggagacg gttgttaagt
      721 atgttcggca ccctaagcac atctttacct gcagtgccat cgaagggagt tttccccact
      781 cggcgaagtt cgctgaatgc cacctatgct taacaaatga tagaatattt ataattcaat
      841 tcataacgta ttaagtggac ttttaatgaa tgtatgattt ggcaatgtcg gtaatatata
      901 tatagatttc ggtggttttc aaggagttat gcccgagtag ttgctgaata tacctgatat
      961 ctgttcctgt ttcgagaaga tgtatgagca aaaccccctc ccccatcctt ccttatcgac
     1021 taggtcgatc tcacattatg gatatttcag tccaactttc tgatctaatc gagagctttt
     1081 cgttggggga ttttcttatt actctttcaa aatccaggcg tttttgtatt tattaggcaa
     1141 ttgctttttg gctgcacgag ctgcttccat ggtttcgtat ttatctaaag cagttctaaa
     1201 ctttccttct ttttcgaaaa taaacgcatt tttgaatcca tcatttatca aatcaatctg
     1261 ggcttgacga gctagttgaa aattcgggta actgccaaat atgagataat attgacctag
     1321 agaagcctga gaaatatctt cacgggtaat attcgattta ctggaaggtt tcgaaacaac
     1381 agaattaata actgaatcag aagagcaaag gcgtatcttt gtttcag
//
LOCUS       DAORPD010000546         1426 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_18097_length_1426_cov_2.225383, whole genome shotgun sequence.
ACCESSION   DAORPD010000546 DAORPD010000000
VERSION     DAORPD010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18097_length_1426_cov_2.225383"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="PK976_06640"
     CDS             complement(<1..314)
                     /locus_tag="PK976_06640"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HRC67598.1"
                     /translation="MKIYDTPYLLSDLIEGNVEFIPLLNGDSGSNLQNEPLPEELSIL
                     PLRNMVLFPGVVLPITVGRSKSIRLVKENYKQNRFIGVVAQRNADTEDPDFNDLYTTG
                     TLA"
     gene            complement(444..>1426)
                     /gene="murD"
                     /locus_tag="PK976_06645"
     CDS             complement(444..>1426)
                     /gene="murD"
                     /locus_tag="PK976_06645"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HRC67599.1"
                     /translation="AGKHSVLLGNIGTPAMSLFDKIRPETSVVFELSSHQLEDVGHSP
                     HISLLLNIFEEHLDHYSSFEEYRQAKYNITRYQKSDDVLIYHSDNSHLQNSVINIKSK
                     AILMPYSMEYKQGMAAWWEKDIICIIDGNETRRIVPEGPLMLRGEHNRSNILAACTAC
                     LLNKIPEKIIGHALPLFEPLPHRLEYVGHIDGKHFYNDSIATIPEATIAAIHTLGKVD
                     TLIVGGYNRGINYEHLIEFLAESEVFHLMLIGEVGKIIEEGLRSTRFNGEIRFYNDFE
                     QLVEDAILITPEGGTCLLSPAAASYDRFINFEERGNLFKNIVLSYSRGMK"
BASE COUNT          397 a          286 c          306 g          437 t
ORIGIN      
        1 gctagtgtgc ctgtagtata cagatcattg aagtcgggat cttcggtatc ggcatttcgt
       61 tgggctacga ccccaataaa cctgttttgc ttatagtttt cttttacaag ccgaatggat
      121 ttactacgtc ctacggtaat gggcagaaca acgccaggaa acagaaccat gttacgaagc
      181 ggaagtatgg ataattcttc gggcaatggt tcgttttgaa gattcgaccc tgaatcgcca
      241 tttaataatg gaataaactc tacattcccc tctattaaat cagaaagtaa gtaaggtgta
      301 tcatagattt tcatagaatt gccattttgt cagataaaaa cctgccccct cccgcaaatt
      361 ccggtagagt ggctggtatg cgttctttag agtgatagac tggtagtcgg ctggataatt
      421 tcaataaata tgccataaat attttatttc atccctctag aataagatag aacaatattt
      481 ttgaaaagat tccctctttc ttcgaaattt atgaatctat cgtaacttgc tgctgccggt
      541 gaaagaagac aagtgccgcc ttcgggtgta ataagaatag cgtcttcgac aagttgctcg
      601 aaatcattgt aaaatcttat ttctccgtta aaacgggttg atcttaagcc ttcctcgata
      661 atttttccca cttctcctat gagcattaaa tggaaaactt cggattcagc cagaaattcg
      721 atgaggtgtt cgtagttgat tcctcggttg taaccaccga cgatcaacgt atctactttt
      781 ccaagggtgt gaatagcggc aatggtagcc tctggaatag ttgcaataga atcgttgtaa
      841 aaatgttttc cgtcgatgtg gcctacatat tccaatctgt ggggcagagg ttcgaaaaga
      901 ggtaaagcat gcccgataat tttttcggga atcttattta acaggcaggc cgtgcacgca
      961 gccagaatat tcgagcggtt atgttcgccg cgtaacatca gtggcccttc cggaacgatt
     1021 cttcttgttt cgtttccatc aattatacag atgatatctt tttcccacca ggctgccatt
     1081 ccctgtttgt attccatgga ataaggcata agaatggctt tcgattttat attaatgacc
     1141 gaattctgca aatgactatt atctgaatgg tatataagaa catcatcaga cttttgataa
     1201 cgagtgatat tgtatttggc ttgacgatac tcttcgaaag acgaataatg gtcgaggtgc
     1261 tcctcgaaaa tattcaagag aaggcttata tgtggtgagt ggcctacgtc ttcgagctga
     1321 tgggatgaaa gttcgaaaac gactgaggtc tcgggtctta tcttgtcaaa caggctcatg
     1381 gccggcgtac caatattccc caataaaacg ctatgtttgc ccgccg
//
LOCUS       DAORPD010000547         1424 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_18144_length_1424_cov_2.693937, whole genome shotgun sequence.
ACCESSION   DAORPD010000547 DAORPD010000000
VERSION     DAORPD010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18144_length_1424_cov_2.693937"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="PK976_06650"
     CDS             complement(<1..632)
                     /locus_tag="PK976_06650"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HRC67600.1"
                     /translation="MEPKDNNYLYNYKELNADHDLKWYDYGARFYDAVIGRWHVVDPM
                     GEISSSWSPYNYVENNPIISIDPTGMASEDALFNAAMNDPNVRSLSGNTNNNQANSDD
                     GEESREIIRDENGMPIRVVIGKKKGNIPTDIEDAENESTTFNCNCGCQGKPPCENDNW
                     TVLSNKEWRNLVWNSKGPLLFLSGKKFNFLKPYGYLGSKTGSSFSSLILG"
     gene            complement(658..1317)
                     /locus_tag="PK976_06655"
     CDS             complement(658..1317)
                     /locus_tag="PK976_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67601.1"
                     /translation="MNNRTKCGDERKKKSKPVGLAYPRFKLYSVLFQNFKPQNLKLTY
                     NFLNLPSKIEWSTTKSIEFIYSASGEKLAKTVKTGATINYIQHYVGGIEYNSASGLNR
                     RVEAIYHGEGRFFNTNTGTTTPTYRTEFSIKDHLGNARVTFTDLDANGKIDVTNNATT
                     NEINQENHYYAFGMAHEGPWMMNDRLILPARQKSSATKALDREGTATNRIAASRNLLW
                     D"
BASE COUNT          342 a          307 c          276 g          499 t
ORIGIN      
        1 ttacccaata ttaaagaaga aaatgaagaa ccagttttgg aacctaaata cccataaggc
       61 ttaagaaagt taaatttttt gccagataaa aataacaatg gacctttact attccatact
      121 aagtttcgcc attctttgtt gcttaaaaca gtccaattat cattttcaca aggaggtttt
      181 ccttgacacc cacaattaca attaaaagtt gtactttcgt tttccgcatc ttcaatatct
      241 gttggtatat ttcccttttt ctttcctata acaactctga ttggcatacc attctcatct
      301 cttatgatct cacgagattc ttcaccatca tctgaatttg cttgattgtt atttgtattc
      361 ccgcttagac ttctaacatt cggatcattc atcgccgcat taaaaagtgc atcctcagat
      421 gccatgccag tagggtctat actaataatt gggttatttt ctacataatt atagggtgac
      481 catgaagatg aaatctcccc catcgggtcc accacatgcc acctgcctat caccgcatca
      541 taaaacctgg ctccgtaatc gtaccacttg aggtcgtggt cggcgttgag ttctttgtag
      601 ttgtagaggt agttgttatc ctttggttcc attggcatta gcagtaaaaa taagctttta
      661 gtcccaaagt agatttctcg atgctgcgat ccggttggtc gcagttccct ccctatcgag
      721 tgcctttgtg gcactcgatt tttgccttgc aggcaaaatc agtcggtcat tcatcatcca
      781 cggcccttca tgcgccatgc cgaaagcata gtagtggttc tcctggttga tttcattggt
      841 agttgcatta ttggttacgt caattttacc gtttgcgtcg agatctgtaa acgttaccct
      901 ggcgttacca agatggtcct ttatactaaa ttccgtgcgg taagtaggtg tagtggtacc
      961 tgtattggta ttaaaaaacc tgccttcacc atgataaatg gcttctaccc tgcggttcag
     1021 gccgctggcg ctgttgtact caatgcctcc tacgtagtgt tggatgtagt tgatggtagc
     1081 tcccgttttt actgtttttg ccaatttttc tcccgatgct gaataaataa attctatgct
     1141 tttggtagta gaccattcta tcttggatgg taaatttagg aaattgtagg tgagttttag
     1201 gttttgtggt ttgaaattct ggaaaagaac tgagtataac ttaaaacgag gatatgcaag
     1261 ccctacaggc ttggattttt tctttctttc atctccgcac ttcgtgcggt tattcacgtg
     1321 gaagcctttg caggcttctt catgtgcatt cgtttttatg taagcccttc aggcttggat
     1381 tttttctttc tttcatctcc gcacttcgtg cggttattca cgtg
//
LOCUS       DAORPD010000548         1420 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_18231_length_1420_cov_2.374359, whole genome shotgun sequence.
ACCESSION   DAORPD010000548 DAORPD010000000
VERSION     DAORPD010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18231_length_1420_cov_2.374359"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="PK976_06660"
     CDS             <1..717
                     /locus_tag="PK976_06660"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC67602.1"
                     /translation="SAFPPEICEGASTQLGTLTQGGTGNYSYIWHPGIYLNDSTLATP
                     TANPPASTEFTVEVFDGTSTANASTTVTVHPLPETPTIILQGNMLVSSSPSGNQWNNE
                     NGPILGATEQNFIPTETGTYYTIVTSEFGCVSSPSNSLYVEVVGITKIPQDAFFLVFP
                     NPFSNLLNISYTLQNSTEVKIEILNSLGQTIANPLIEQKSEAGSYQIQLNTTSFKPGI
                     YYIRFTTGTSSIVVKALLMK"
     gene            857..>1420
                     /locus_tag="PK976_06665"
     CDS             857..>1420
                     /locus_tag="PK976_06665"
                     /inference="COORDINATES: protein motif:HMM:NF019737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family peptidase propeptide
                     domain-containing protein"
                     /protein_id="HRC67603.1"
                     /translation="MKRLLLLFGLLFCMLASRAGEWIPLTSEEPQKAGIHLISSDITS
                     SVIEFQIKGFYLKPVQTPRGIENIVEVGNSSRILAAGAPDLPKLTASVIIPDEAMMGI
                     RILSSSYTDYTGIEVAPSKGNFTRDIDPASVPYTYGPEYQSNHFFPQTEGELREPYIL
                     RDFRGQTLIVYPFFYNPVTKVLRVFSEL"
BASE COUNT          415 a          349 c          241 g          415 t
ORIGIN      
        1 tcggcattcc ctcccgaaat ttgcgaagga gcttctacac agctaggaac tttgacacaa
       61 ggtggcacag gaaattattc ctacatctgg catccaggaa tttatcttaa cgactccacc
      121 cttgctactc caaccgctaa tcctcctgcc tcaaccgaat ttacggtcga agtattcgac
      181 ggaacctcaa ctgctaacgc ctcaacaact gttactgttc accctttgcc cgaaaccccc
      241 actattatct tacagggaaa tatgctggtt tcaagctctc catcagggaa tcaatggaat
      301 aatgaaaacg gccctatttt aggagctacc gaacagaact ttatcccaac cgaaactgga
      361 acctactata ccatagtaac cagtgaattt ggatgtgttt cttcacccag caattcactt
      421 tatgtcgaag tagtgggaat aacaaaaatt ccacaggatg ctttcttcct tgtctttcca
      481 aatcctttca gcaatctttt gaatatctcc tataccttac aaaattcaac tgaagtaaag
      541 atcgaaatcc tcaactccct gggacaaacc attgctaatc cacttattga acaaaaatca
      601 gaagccggat cgtatcaaat tcaattgaac accacctcct tcaagcctgg aatctattac
      661 atacgattta caacaggaac cagttcaatt gtggttaaag cccttttaat gaaataatcc
      721 tacaaacttt accttttaaa accctgagat ccattctcgg ggttttttat tccctaatgt
      781 tttcaaaaat taacaggatt aatgtaaatt tgtcgaatga aattttttta aactgaatta
      841 ccaaacttta caccccatga aacgtcttct tctactattt ggcttgttgt tttgcatgct
      901 ggcgagcaga gccggtgagt ggatacctct gacctccgag gaaccacaga aagcaggcat
      961 tcatctgatt agctctgata ttacttcatc tgtgatcgaa ttccagatca aaggtttcta
     1021 tctgaagcct gtacaaacac ctagaggtat cgaaaatatt gtcgaggtgg gcaattcttc
     1081 tcgcatactg gcggccggtg cgcccgatct tcccaagtta actgcttctg taatcatccc
     1141 cgatgaagct atgatgggaa ttagaattct ctcatcttct tataccgatt acacgggtat
     1201 agaagtggct ccgagcaaag gaaattttac tcgcgacatc gatccggcat ctgtgccata
     1261 tacctatgga cctgaatatc aatccaacca tttcttcccc caaacagaag gggaattgcg
     1321 tgagccttat atattacgcg attttcgtgg ccaaaccctc atagtttacc catttttcta
     1381 taatccggtt actaaagttt taagagtgtt ctccgaactt
//
LOCUS       DAORPD010000549         1414 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_18351_length_1414_cov_2.188374, whole genome shotgun sequence.
ACCESSION   DAORPD010000549 DAORPD010000000
VERSION     DAORPD010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18351_length_1414_cov_2.188374"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="PK976_06670"
     CDS             <1..222
                     /locus_tag="PK976_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67604.1"
                     /translation="PNTGFCSTYSISIDAGSGFKSYLWSTGASSQVINVTQGGSYQVT
                     VTDWNNCSNSDSIQVIEHPLPSPLQIKHK"
     gene            632..>1414
                     /locus_tag="PK976_06675"
     CDS             632..>1414
                     /locus_tag="PK976_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67605.1"
                     /translation="MDENFKIAPESINCTTDETKTACQQIIAVFDTIECIPVPQDTGL
                     FFYFRVCLGDSVFFKIKGIYPQNDNLYHQSDSLSIFTWNFGDGTKIVTDTPCIWHTYD
                     TAKGFEVSVYIIDTNDCLSLPLIGRVTVTSNPINQVKPMPEICLNDMVTLHASTFTTY
                     SSYSYSQITSQKFDSLMFIPDGPNCNPTNPCYNTDVFFTSFLPNQTITSADDILSICV
                     FMEHSYVGDLNFRIICPNGQNDTLKEYIHWGGADMGIPGTPDQ"
BASE COUNT          448 a          293 c          217 g          456 t
ORIGIN      
        1 ccaaatacgg gcttttgctc tacctattcg atttccattg atgcaggttc aggttttaaa
       61 tcctatttat ggagcacagg tgctagttcg caagtcatta atgttactca gggtggatcg
      121 tatcaagtaa cagttactga ttggaacaac tgctccaatt ccgattctat ccaagtaata
      181 gaacatcctc ttccgtctcc ccttcaaatc aaacacaaat gatatcttct cctcaagata
      241 tagcctcatt aatatccaat cttttgtaat tcaatgaaat aatttttatt aaagccatac
      301 ttcgtaattc agaaaaaata ctctattcag agggattata atcccccttc aatctaaaag
      361 ccatcgacaa ttattggaat agaaaatctc aaggaacaaa atgaaactta actaaccaaa
      421 tcattcgaca ttaaaataac attcgatgtt ttctcgtaat tttgaagcga aatatttgag
      481 aacacactat ttacctttct cttgtctttt ttctatgact cacttacaat caaatgatgc
      541 ggcaattcat ccaatcttaa tcgtaattct cattcttctt gcaagtaaag tttattctca
      601 ggacttaata ataaatggaa agcagccttt tgtggatgag aatttcaaaa ttgcaccgga
      661 aagcattaat tgtacaactg acgaaaccaa aaccgcttgc cagcaaatca tagcagtatt
      721 cgatacaata gagtgtatcc ctgttcctca agacactggt ttattctttt atttcagggt
      781 ttgtttgggt gatagtgtat tttttaaaat aaaaggtatc tatccccaaa acgataatct
      841 ttatcatcaa agcgacagcc tttctatctt tacctggaat tttggagatg gaactaaaat
      901 tgtaaccgat actccctgta tatggcacac atacgataca gccaagggat tcgaagtgag
      961 tgtatatatc atcgatacca atgattgcct tagtctgccc ttgattggac gggttactgt
     1021 gacttccaat cctatcaatc aagttaagcc aatgccagaa atttgtttaa atgatatggt
     1081 tacccttcat gcttctactt ttactactta ttcatcatac agttacagcc aaattacaag
     1141 ccagaaattc gatagcttga tgtttattcc cgatgggccc aactgcaatc ccacaaatcc
     1201 ttgctataat actgacgtat tttttacttc atttttaccc aatcaaacca taacaagtgc
     1261 cgatgatatt ctttccatct gtgtattcat ggaacattca tacgtaggcg acttgaattt
     1321 tcgaatcatc tgtcctaatg gacaaaatga tactttaaaa gaatatattc attggggggg
     1381 tgccgacatg ggaatacccg gaacccctga ccaa
//
LOCUS       DAORPD010000550         1413 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_18377_length_1413_cov_2.455817, whole genome shotgun sequence.
ACCESSION   DAORPD010000550 DAORPD010000000
VERSION     DAORPD010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1413
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18377_length_1413_cov_2.455817"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /gene="upp"
                     /locus_tag="PK976_06680"
     CDS             <1..587
                     /gene="upp"
                     /locus_tag="PK976_06680"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF001097.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HRC67606.1"
                     /translation="IQIDSLRFRTNMERASIIFAYEISKKFRYKKKEVTTVLGTAEVS
                     VPIDTLVITSILRAGLPMHNGMLHVFDHAENAFISAYRKYDRDGGFHVEVEYVSSPDL
                     THKILILCDPMLATGSSMVLCYKELLNHGKPLHTHFVSLISSVEGIEFLKQQIPPDNY
                     TLWTGAIDDELTAQAYIVPGLGDAGDLAYGKKVK"
     gene            609..>1413
                     /locus_tag="PK976_06685"
     CDS             609..>1413
                     /locus_tag="PK976_06685"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRC67607.1"
                     /translation="MKLKNAVFYENIRIALASVRSHLVRSFLTISIIAFGIMALVGIL
                     TSIDAIKNFFEENFMHMGANTFTIRNLRIQSESERGGRIGEPIRYNQAISFKERFEYP
                     AVVSVSVNASFSTTAKYKNIKTHPNLRVLGIDENYLITSGEELRVGRSFTSNEVQSGS
                     FVCIIGNDVANKLFKGINDPTGSVITIGPGKYTVVGVLAQSGSSFGFSGDRNIYLPLN
                     NVRQRYSLNNVSYLINIWVSSATELEAAIGETTGFFRQIRNLRLPESNNF"
BASE COUNT          407 a          262 c          318 g          426 t
ORIGIN      
        1 ccatacagat tgatagtctg cgttttcgta ccaatatgga aagagcttcg ataatttttg
       61 cctatgaaat cagtaagaaa ttcaggtaca aaaagaaaga ggttacaact gttttgggaa
      121 ctgctgaagt ttcggtgcca atcgatacat tggttataac gagcatactg agggccggct
      181 tgccaatgca taatggcatg ctgcatgttt tcgatcatgc cgaaaatgcc tttatttctg
      241 cttatcgcaa atatgatcgt gatggtgggt ttcatgtcga agtggagtat gtttcatcac
      301 ctgatctgac gcataagatt cttattcttt gcgatcccat gctggccaca ggatcgagca
      361 tggtattgtg ctataaagaa ctgctcaatc atggcaaacc attgcacacc cactttgtct
      421 ctctcatttc gagcgtggaa gggattgaat ttctaaagca gcaaattccg cccgataatt
      481 atactctatg gacaggtgca atagatgatg agcttactgc tcaagcctat attgtcccag
      541 gattaggcga tgcaggagat ctggcttatg gcaagaaagt aaaataaccc ggtaatttta
      601 cctgaagcat gaaattgaaa aatgctgtat tctatgaaaa tataaggatt gcgcttgctt
      661 ctgtacgcag ccatttggtt cgatcgttcc ttaccatcag cattattgcc tttggcatca
      721 tggctttggt cggaatcctt acttcgatcg atgccatcaa gaattttttt gaggaaaatt
      781 tcatgcatat gggcgctaat acttttacaa tacgaaatct gagaattcaa tcggaaagcg
      841 agcgtggagg ccgtattgga gaacctattc gctataatca ggccataagt tttaaagaaa
      901 ggttcgagta tccagccgtg gtctctgttt cagtaaatgc gtccttcagc acgactgcca
      961 aatataagaa cataaaaact catcctaatc tgagagtttt gggaatcgac gagaattatc
     1021 tcattacttc cggcgaagaa ctgcgagttg gaagaagttt taccagtaat gaagtacaat
     1081 ctggaagttt tgtttgtatt attggaaatg acgtggctaa taaacttttt aaagggatta
     1141 acgatcctac agggagcgtg attacgatag gacctggcaa atacacggtt gtgggggttc
     1201 tagcgcaaag tggttcgagc tttgggtttt cgggcgatcg aaatatttat ctccctttaa
     1261 ataatgtccg ccagcgttat tccttaaata atgtttctta tttgataaat atatgggttt
     1321 cgagtgctac agaattagag gcagctattg gagaaactac agggtttttc cgtcaaattc
     1381 gtaacttaag gctgccggaa agcaacaatt tcg
//
LOCUS       DAORPD010000551         1412 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_18402_length_1412_cov_1.929993, whole genome shotgun sequence.
ACCESSION   DAORPD010000551 DAORPD010000000
VERSION     DAORPD010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1412
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18402_length_1412_cov_1.929993"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="PK976_06690"
     CDS             complement(<1..724)
                     /locus_tag="PK976_06690"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRC67608.1"
                     /translation="MRRKTFGFIAVFVGLSLIICKTHAQNSSDPVLMTIDGQPVRVSE
                     FLNIYSKNNVQSEVADKKSMEEYLELFINFKLKVRDAEVNGLDTVKVLKDELAGYRKQ
                     LSASYFIDSVVFRKLVNEAYERMQWDLRASHILLRCDKDASPSDTLAPWKRMDEILRK
                     ACSGEPFERLAIQYSEDPSARDREATSQHPFIKGNRGDLGFFTVFNMVYPFETGAYRT
                     QPGKISDIVRTDFGYHIINVTDK"
     gene            complement(763..>1412)
                     /locus_tag="PK976_06695"
     CDS             complement(763..>1412)
                     /locus_tag="PK976_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HRC67609.1"
                     /translation="DIVVGNIATRQAAEDLLKLDIDAIKVGIGPGSICTTRIIAGIGV
                     PQLTAIMEVYDVIKDTPVPLIADGGIRFTGDIVKALAAGASTIMAGSLFAGVDESPGE
                     AVIYEGRKYKTYRGMGSIEAMQKGSKDRYFQDMEDDIKKLVPEGIVGRVPYKGSLNEV
                     VTQMVGGMRSGMGYTGCKNISELRKATFIRITQAGIIESHPHDVTIISEAPNYSR"
BASE COUNT          397 a          331 c          273 g          411 t
ORIGIN      
        1 gtttatcggt tacattgatg atgtgataac caaaatccgt tcgcacgata tcggagattt
       61 tgcctggttg agtgcggtat gcacctgttt cgaaaggata aaccatattg aaaacagtga
      121 agaaacccaa atcgccgcga ttgcccttga taaaaggatg ttgtgaagtg gcttcgcgat
      181 ctcgggccga aggatcttcg gaatattgaa tagcaagccg ctcgaaaggc tcgcctgaac
      241 aggcttttcg caatatttca tccattcttt tccagggagc aagcgtgtcg gaaggcgaag
      301 catccttgtc gcaacgaagc aggatatgac tggcccgcaa atcccattgc atacgttcgt
      361 aagcttcatt taccagtttc cggaaaacga cagaatcgat gaaataggac gccgataatt
      421 gtttcctata tccagccagc tcgtctttca aaacctttac cgtgtcgagt ccattaactt
      481 ctgcatctct aactttcaat ttgaaattga taaaaagttc gagatattct tccatcgatt
      541 ttttatcagc tacttctgac tgaacattat tctttgaata gatattgaga aattcactca
      601 ctctgacggg ttgtccgtca atggtcatca aaacaggatc actcgaattt tgagcatgtg
      661 ttttacaaat gatcaaggaa agacctacaa aaactgcaat aaacccaaaa gtttttcttc
      721 tcatatatat acaattttat ttattgattt tcttcatgaa gattaacggc tataattagg
      781 agcctcactt ataatagtta catcgtgagg atggctttcg atgattccgg cttgggtaat
      841 acggatgaag gtagcctttc gaagttccga tatatttttg caaccagtat aacccatacc
      901 cgatctcatg cctcccacca tttgagtaac cacttcattg agagaacctt tatagggaac
      961 tcttcccact ataccttcgg gaaccaattt cttaatatcg tcttccatat cctgaaaata
     1021 acgatccttc gaacctttct gcatagcctc aatggagccc atacctctgt aagtcttata
     1081 ttttcttccc tcgtaaatta cggcttcgcc gggtgattca tccacgcccg cgaacaaaga
     1141 acctgccatg attgtggacg cacctgctgc aagggccttt acgatatctc ccgtaaagcg
     1201 aatacctccg tcagcaatca aaggcacagg ggtatctttt atcacatcat aaacctccat
     1261 gatggcagta agttgaggaa cgcctattcc ggctataatt ctggtcgtgc agatggaacc
     1321 cggtccaatc cccaccttga tggcatcaat atctaacttc aaaaggtctt cggctgcttg
     1381 gcgagttgca atattaccta ccacaatgtc ga
//
LOCUS       DAORPD010000552         1410 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_18453_length_1410_cov_1.770480, whole genome shotgun sequence.
ACCESSION   DAORPD010000552 DAORPD010000000
VERSION     DAORPD010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1410
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18453_length_1410_cov_1.770480"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(446..634)
                     /locus_tag="PK976_06700"
     CDS             complement(446..634)
                     /locus_tag="PK976_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67610.1"
                     /translation="MVNPENEKKLDKITDSKTGRDSYTNLMPSVQSLHILRGSCTKPI
                     ACPFYQVPFIHCRKTAWN"
     gene            751..1170
                     /gene="rpsL"
                     /locus_tag="PK976_06705"
     CDS             751..1170
                     /gene="rpsL"
                     /locus_tag="PK976_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HRC67611.1"
                     /translation="MPTIQQLIRRGRQKLEYKSKSPALDSCPQRRGVCTRVYTTTPKK
                     PNSAMRKVARVRLTNGNEVTAYIPGEGHNLQEHSIVMIRGGRVKDLPGVRYHIIRGAL
                     DTAGVEGRMQRRSKYGAKRPKTKPGAASPKDTKAGKK"
     gene            1216..>1410
                     /locus_tag="PK976_06710"
     CDS             1216..>1410
                     /locus_tag="PK976_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HRC67612.1"
                     /translation="MRKAKPRKRIILPDPKYNDTMVTKFVNNIMLTGKKSIAFDLFYE
                     AVDIVSQKTGEDGLEVWKKAL"
BASE COUNT          467 a          229 c          297 g          417 t
ORIGIN      
        1 cgttggacga aaagtccttc cattggatct tctttgtggg ggtaccagcg cgaaacgaac
       61 aagatacgaa gtttttcttc agtcatcctt cttaggtgtc aattgctggc aaaactacca
      121 aaaatcaaga gagttgatat tttacagcct gattcggttc attttttaaa tattgagcaa
      181 tcggtacatt aacaataaaa attttttgta atttattata aatcaataat ctaattgaaa
      241 ttaataataa ttaacagtct atatttcgat agaaattctg ggaagcagat gaaatcttag
      301 cattatcaaa ttactgatgc ggttgatgtt ataattattt ttaatgttgg atcgatgaaa
      361 atcctataaa aaatcaaagg aatgattttg tagaatgaaa aaactatgta attttgcacc
      421 cgctttaaaa aagaagcttt tttgattaat tccatgccgt tttacggcaa tgaatgaaag
      481 gaacctgata aaacggacaa gctataggtt tcgtgcatga acctctcaga atgtgaagtg
      541 attgaacaga tggcataagg ttcgtgtaac tgtccctacc ggttttgcta tcggtaattt
      601 tatccagttt cttttcattt tcaggattaa ccaatctttt ttgagataaa ttttaatgaa
      661 gatcattggt cgtaaaagat ttaattgtac ttttgcactc cctttttgag agggtgctta
      721 aaaaccagat aaggaactaa taaagttgat atgcccacta tacaacagtt gattcgtaga
      781 ggacgtcaaa agcttgagta caagagcaaa agcccagctt tggattcatg tcctcaacgc
      841 agaggagttt gtacaagagt gtataccaca actcctaaga agcccaattc ggctatgcgt
      901 aaggtagcaa gggtgcgact gaccaatggt aatgaagtca cggcttacat tcctggcgag
      961 ggtcataatt tgcaggagca ctctattgta atgattcgcg gaggcagagt aaaggatcta
     1021 ccgggagtca ggtatcatat tattcgaggt gctttagata ctgcgggtgt tgaaggccgt
     1081 atgcaacgtc gttcgaaata tggtgcaaag cggcctaaaa ctaagcctgg tgctgcaagt
     1141 cctaaagata caaaagctgg caagaagtaa attcgttgtt tagaaaatac aattcgcaat
     1201 agattataat agattatgag gaaagcgaaa ccaagaaaaa gaatcatact tcccgacccc
     1261 aagtacaatg atacaatggt taccaagttt gtaaataata tcatgctcac ggggaagaaa
     1321 agtattgctt tcgacctttt ttacgaagca gtggatattg tgagtcagaa aacgggtgag
     1381 gatggcctcg aagtctggaa aaaagctttg
//
LOCUS       DAORPD010000553         1408 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_18498_length_1408_cov_1.940872, whole genome shotgun sequence.
ACCESSION   DAORPD010000553 DAORPD010000000
VERSION     DAORPD010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18498_length_1408_cov_1.940872"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            253..>1408
                     /locus_tag="PK976_06715"
     CDS             253..>1408
                     /locus_tag="PK976_06715"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HRC67613.1"
                     /translation="MKTDIEIAHEVKLKHINEIAGMLGINPEDLVLYGKYKAKIPLNY
                     INYDKIKGNKLVLVSAISPTPAGEGKTTVSIGLAQALNRIGKKTTAVLREPSLGPVFG
                     VKGGATGGGYSQVLPMEDINLHFTGDFAAIERAHNLLAALIDNNIQSKKRSLGIDPRT
                     VKWKRVMDMNDRSLRRIIVGLGGTTNGVPRETGFDITAASEVMAILCLAENLQELKNR
                     LGNIFIGYTYDKKPIFARDLNANGAMTALLKDAFMPNLVQTLEGTPAIIHGGPFANIA
                     QGTNTVVATKMGLSLSDFVVTEAGFGFDLGAEKFIDIKCQYAGLFPCSVVLVATVRAL
                     KYHGGKPLKDLSQPDIEALKKGIGNLEKHIENMKLFGIHPIIALNRFISDT"
BASE COUNT          457 a          294 c          290 g          367 t
ORIGIN      
        1 atcaactcga ctttgcctca caaattgtga attaagataa aagctatcat caggaccgtc
       61 tgaatgaata ctcttcttct gattccataa atcttataaa actaaaaaaa atctttctcg
      121 ttttgagcca aaaataagag tacttttgcc tctctaagat aaggatatct atacattgat
      181 aattaaaatg caaataattc cgaaactgag gggaaagtat taataaataa ataatattaa
      241 aactctatcc atatgaaaac agatattgaa attgctcatg aagtaaagtt gaaacacatc
      301 aatgaaattg caggaatgct aggaatcaat cccgaagatc tggttctcta cggtaaatac
      361 aaagcaaaaa tcccattaaa ttatatcaac tatgataaaa taaagggaaa caagctggtt
      421 ctggtatctg ccatttcgcc cacgcctgca ggcgaaggga aaactaccgt ctcgattggg
      481 ctggctcaag ccttgaaccg tattggcaaa aagaccactg cagtattgcg tgagccttca
      541 ttaggtccag tatttggtgt gaaaggagga gctacaggtg gggggtattc acaggtcctt
      601 cccatggaag atatcaatct gcattttacg ggggattttg ctgcaatcga aagagcacac
      661 aatcttcttg cagctttaat tgataataac atccaaagta aaaagcgctc gctgggaata
      721 gatccacgca cagtgaaatg gaaaagagta atggatatga acgaccgttc gctgcgacgt
      781 atcattgtgg gcttgggtgg gaccacgaat ggggtacctc gtgaaacggg cttcgatatt
      841 actgcagcct ccgaagtaat ggccatcctt tgcctggccg aaaatctcca ggaactgaaa
      901 aatagactcg gcaacatttt cattggttac acatacgata aaaaacccat ctttgctcgc
      961 gacttaaatg caaatggcgc aatgacagcc ttactgaaag atgcctttat gcctaacctc
     1021 gtgcaaactc tcgaaggcac tccagccatt attcatggag gaccatttgc caatattgcc
     1081 caaggaacca acacagtagt agctaccaaa atgggactat ccttaagtga ttttgtcgtt
     1141 acagaggctg gtttcggttt tgacttgggt gcggaaaaat ttatcgacat caaatgtcaa
     1201 tatgccggct tgttcccatg ctcggttgtt ctcgtggcta cggtgagagc actgaaatat
     1261 catggtggga aaccattgaa agacctttcc caacccgaca ttgaagcgct gaaaaaaggc
     1321 atcggaaatc tcgagaaaca catcgaaaac atgaaactgt tcggcatcca tcctattata
     1381 gctcttaacc gctttataag cgacactc
//
LOCUS       DAORPD010000554         1404 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_18582_length_1404_cov_2.074870, whole genome shotgun sequence.
ACCESSION   DAORPD010000554 DAORPD010000000
VERSION     DAORPD010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18582_length_1404_cov_2.074870"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="PK976_06720"
     CDS             <1..291
                     /locus_tag="PK976_06720"
                     /inference="COORDINATES: protein motif:HMM:NF024823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRC67614.1"
                     /translation="VCFTKVIQIEPKNMYAYYNRGIALSNMNKYKEAIADFDVALHID
                     STYSPALNNRGNAKFMSGDPDGACKDWKKSIRLGNIASEKAYNHYCLKNKIR"
     gene            483..>1404
                     /locus_tag="PK976_06725"
     CDS             483..>1404
                     /locus_tag="PK976_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67615.1"
                     /translation="MKKKHCLFIIFIAVFFPQLVFSQQMTVKRAAYFRKSQPLSEMAA
                     VLPGARDRSWKDSLVRNLAFDEHHPIMHQLTPAQLDPVRQISAPSIPNAGPILNFAGI
                     GNVNSVYPPDTEGDVGRDYYFQMINLSFAIYDKQGNLLFGPVNNSTLWNGFIGPWTGT
                     NDGDPIVLYDELANRWIASQFAVNTSNGTYWQLIAISETSDPLGSYYQYAFEFPAFND
                     YPKFGIWPDAYYASFNLFGSYVRVAAAAFERDAMLVGDSNARMILFDLPPNAGAWSML
                     PSDFDGNPPPTGAPNYFAYFTDDGLGALQDA"
BASE COUNT          399 a          281 c          280 g          444 t
ORIGIN      
        1 gtttgtttta cgaaagtcat ccagatcgag cctaagaata tgtatgccta ttataataga
       61 ggaattgcat tgagtaatat gaataaatac aaagaagcca tcgctgattt cgatgtagca
      121 ttgcatatcg attcgaccta ttctcctgcg ttgaataaca gaggaaacgc caaatttatg
      181 agtggcgatc cggatggtgc ttgcaaggat tggaaaaaat ctatacgcct gggaaacata
      241 gcttccgaaa aggcctataa tcattactgc ctgaaaaata aaattcgata gagcaagcaa
      301 agatgaatcg gaatatacat tcgatgatat caattgaatc tatccatgct tagaaagaat
      361 ctcctttgga aaggtaaata ataaacttct ccaccttttt gaaatcaacc taactttatt
      421 atatcttact gaaatgttta tgtaaatttg tgccggataa gaatttacca attttactac
      481 tcatgaaaaa gaaacattgc ttattcatca ttttcattgc tgtctttttt ccccaactgg
      541 ttttttcaca acaaatgacg gtaaagagag cagcatattt cagaaaatcg cagcctttgt
      601 ctgaaatggc agctgttttg cccggagcga gagatcgttc ctggaaagat tcactggtgc
      661 gcaatcttgc ttttgatgaa caccatccca tcatgcatca gctcacacct gctcagctcg
      721 atccggtaag gcagatatct gccccttcga ttccgaatgc gggtcccata ttgaattttg
      781 ctggaatagg caatgtaaat agtgtttatc cacctgacac cgaaggagat gtaggccgcg
      841 attattattt ccagatgata aatctttcat ttgctattta cgataaacaa ggtaatttgc
      901 tttttggtcc tgtaaacaat tcgaccctat ggaatggttt tattgggcct tggactggta
      961 ccaatgatgg cgatcctata gtcctatacg atgaattggc caatagatgg atagcctcac
     1021 aatttgccgt gaacacatcg aatggaactt attggcaact tattgccatt tcagaaactt
     1081 ccgatccttt aggttcatat tatcaatatg catttgaatt ccctgctttc aacgattatc
     1141 caaaatttgg catctggcct gatgcttatt atgcttcttt caatttgttt ggaagttatg
     1201 taagagttgc agctgctgct tttgaacggg atgctatgct ggtgggggat tccaacgctc
     1261 gaatgattct ttttgatttg ccacctaatg ctggggcatg gagcatgctt ccctccgatt
     1321 tcgatgggaa tcctcctcca acgggcgctc ccaattattt tgcttatttc accgatgatg
     1381 gtttgggagc tcttcaagat gcct
//
LOCUS       DAORPD010000555         1402 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_18616_length_1402_cov_2.530809, whole genome shotgun sequence.
ACCESSION   DAORPD010000555 DAORPD010000000
VERSION     DAORPD010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18616_length_1402_cov_2.530809"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="PK976_06730"
     CDS             <1..299
                     /locus_tag="PK976_06730"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRC67616.1"
                     /translation="REGEGPRIREGQEIALDYKMYLLTGDLVRESKPGNPLRFTVGKS
                     DIISGLDEAVRLFRKGTIAKIIIPSHLAYGATGDQQYIPPRATLVLDIKIVEPY"
     gene            311..1171
                     /locus_tag="PK976_06735"
     CDS             311..1171
                     /locus_tag="PK976_06735"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRC67617.1"
                     /translation="MKTKITYFFALTLIAVTLISCSKYPGYKKTDSGLYYKFYVKSGD
                     TAKPKVNDILKLYMSYGTKDSLLMDGKEEFLLQLTNPQFKGDINEALAMMSKGDSASF
                     IMPADSFFKKIVHVPLPDFIDSTEMLYFNVKMIDFFSQEEFIKRMNEENALKEQKELS
                     DLESYLSANNITVQPTESGLIYIEIVKGTGPQATQGKKAKVHYTGTLLDGTKFDSSYD
                     HPDKQPLEFIIGEQRIIPGFEEGVSLMKKGGKAKLIMPSKLAYGSRPAGPSIPAFSTL
                     VFEVELIDVQ"
     gene            1193..>1402
                     /locus_tag="PK976_06740"
     CDS             1193..>1402
                     /locus_tag="PK976_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67618.1"
                     /translation="MSIRHLRFILFIFVIGCIVYGCSRFSGYKKTSDGLYYKFYRQNE
                     GPHPDTSNIVQINLSYRYGDCVLFSS"
BASE COUNT          426 a          265 c          296 g          415 t
ORIGIN      
        1 taagggaagg agaaggccca aggattagag aagggcagga aattgctctt gattataaaa
       61 tgtatttgct cacaggcgat ctggttcgag aatcaaagcc aggaaatcct ttacgattta
      121 ccgtaggcaa atccgatata atttctggcc ttgatgaggc cgttagattg tttcgaaaag
      181 gcacaattgc aaaaattatt ataccttcgc acttggctta cggagctacg ggtgatcagc
      241 aatatatccc tccacgagcc actttagtgc tcgatattaa aattgttgaa ccttattaac
      301 acattacatc atgaaaacta aaattaccta tttttttgct cttaccttga tagctgttac
      361 cttgatttcg tgtagcaaat atcctggtta caagaaaacc gattcaggtt tgtattacaa
      421 gttttacgtc aagtcgggcg atactgccaa acctaaggtt aacgacatct tgaaacttta
      481 tatgtcgtat ggtactaaag acagtctttt gatggatggc aaagaggaat ttcttctgca
      541 gttaaccaac ccacaattca agggtgatat caatgaagcc cttgccatga tgtcgaaagg
      601 cgatagtgca agtttcatca tgcctgccga ttcatttttc aagaagatag tacatgttcc
      661 gttgcctgac tttatcgaca gcacagaaat gctttacttt aatgtaaaaa tgatcgattt
      721 tttcagtcag gaagaattca tcaagcgtat gaatgaggaa aatgcgctca aagaacagaa
      781 ggagctaagc gatctggaaa gttatcttag cgctaataat attacggtgc aacctaccga
      841 gagcgggctt atctacatcg agatcgttaa gggaactggt ccacaagcaa cccaagggaa
      901 gaaggccaag gttcattata caggaacctt gctcgatgga accaagttcg attcatcata
      961 cgatcatcct gataaacaac ctttagaatt tataataggt gaacagcgta ttattcctgg
     1021 tttcgaagaa ggtgtaagtt tgatgaaaaa aggaggcaaa gccaaattga ttatgccttc
     1081 caagcttgct tatggcagcc gtccggctgg gcctagtatt cctgcattct ctactctggt
     1141 atttgaggtt gaactcattg atgttcaata attttgaacg tttttgcatg atatgagcat
     1201 tcgccattta cgtttcattt tatttatttt tgtcatcggc tgcatcgttt atgggtgcag
     1261 ccgatttagt ggttataaga aaaccagcga cggtctatat tacaagtttt accgacaaaa
     1321 tgaaggcccg catccagata cgagtaatat cgtacagata aatctaagtt atcgctacgg
     1381 ggattgcgta ttatttagca gc
//
LOCUS       DAORPD010000556         1402 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_18619_length_1402_cov_2.474388, whole genome shotgun sequence.
ACCESSION   DAORPD010000556 DAORPD010000000
VERSION     DAORPD010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18619_length_1402_cov_2.474388"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1402
                     /locus_tag="PK976_06745"
     CDS             <1..>1402
                     /locus_tag="PK976_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005784705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRC67619.1"
                     /translation="KADADARPIMHIAIHSPKRSLLELSEIAELTFKEQLQTISGVSA
                     VNIWGEKRYAMRIWLDPSKLAGYQMTPLDVRNSLQRENIELPAGSIEGNNTELTIRVL
                     GLMSSPEEFNNLILKRSGDQLIRVKDIGRAELSPEDLRSIMKMNGIPMVATVIVPQPG
                     ANQIEIVDEVYKRLDFIKKDLPQDIGIEFGFDNTQYIRSSIKEVQTTIYVAFFLVVSI
                     IFLFLRDWRTTLIPILAIPVSLIGTFFIMYLAGFSINILTLLGIVLSVGLVVDDAIVM
                     MENIYVKIEQGMSPWEAAIKGSNEIFFAIIATTITLIAVFFPIVFLQGMTGRLFREFS
                     VVIAGAMAISAFVALTFTPMLSSKMLKARPSHGKLYDFTEIFFIRLNAGYKNSLDNFL
                     RRKYLPLTIVGGTIVLIIVLWKVIPAEMAPLEDRSQINLRMIFPEGSTYDYSFAYTEE
                     ISALVDKLVPEAEKSTM"
BASE COUNT          405 a          298 c          293 g          406 t
ORIGIN      
        1 gaaagccgat gccgatgccc gacctatcat gcatattgca atccatagtc ctaaacgatc
       61 gttgctcgag ctatctgaaa tagctgaact aacctttaag gagcagttgc aaaccatctc
      121 gggagtcagt gccgtaaata tttggggaga aaaacgatat gccatgcgta tatggctcga
      181 tccttccaaa ctggccggat atcaaatgac acccctcgat gttagaaatt ccttgcaaag
      241 agaaaatata gaattgccag cgggtagcat cgaaggaaat aataccgaac ttacaatacg
      301 tgttcttgga ttgatgagca gcccagaaga attcaataat cttatcctga aacgttcagg
      361 cgatcaactg attagagtaa aagacattgg ccgtgccgaa ctttcgcccg aagacctccg
      421 tagcatcatg aagatgaacg gaattcctat ggttgctacg gtaattgtgc ctcaacccgg
      481 cgctaaccaa atcgaaatcg tggatgaagt atacaaaaga ttggacttca tcaaaaaaga
      541 tcttcctcaa gacatcggaa tcgaatttgg tttcgataac acacaataca tacgctcatc
      601 catcaaagaa gtccaaacca ccatttatgt ggcatttttc ctcgtagtat ctattatttt
      661 tcttttcttg cgcgattggc gtactaccct tattcctatt ctcgcaatcc cagtttcact
      721 cattggtacg ttttttatca tgtatctggc tggctttagc attaatattc ttaccctgtt
      781 gggaatagta ctttcggtag gtttggtggt tgacgatgcc atagtgatga tggaaaatat
      841 ctatgtaaag attgaacaag gcatgagccc atgggaagca gccatcaagg gatcgaacga
      901 gattttcttc gccatcatag ctaccaccat tactttgatt gcagttttct ttcccatagt
      961 tttcctgcaa ggcatgacag gaaggctctt tcgtgaattc agtgtcgtca tagcaggtgc
     1021 aatggcaata tcggcatttg tagcgcttac atttactccc atgctttcgt cgaaaatgct
     1081 gaaagccagg ccgtcacatg gaaaactcta tgattttact gaaatattct ttattcgatt
     1141 gaatgctgga tataaaaatt cactggataa ttttctgcgc aggaaatatt taccattgac
     1201 gatagtgggg ggtacaattg tcctcataat cgtcctatgg aaagtgatac cggccgaaat
     1261 ggctcctctg gaagaccgtt cccagatcaa tctgagaatg attttccccg aaggttcgac
     1321 ttatgattat agttttgctt atactgaaga gatttcggct ttggtggata aactcgttcc
     1381 tgaagcagaa aagtcaacca tg
//
LOCUS       DAORPD010000557         1402 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_18623_length_1402_cov_1.888641, whole genome shotgun sequence.
ACCESSION   DAORPD010000557 DAORPD010000000
VERSION     DAORPD010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18623_length_1402_cov_1.888641"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="PK976_06750"
     CDS             complement(<1..759)
                     /locus_tag="PK976_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil-5-)-methyltransferase RumA"
                     /protein_id="HRC67620.1"
                     /translation="MRNRNLPIIERLEITDAGAEGMAVGRFQDMVIFVPWAVPGDIVD
                     VRVVRKRKRFWETRLIRIIKESENRRKPWCSHFGICGGCRWQNMDYSTQLFYKAKWVN
                     DTLLRLGKFEFPEPLPILGSPGKVSYRNKMEFTFSNRRFLDEGENADDPEITLQALGL
                     HLPDRFDRVLDIQHCYLQPESSNKIRLAVKRFALEHGLVFQDLVTHHGFLRNLIIRNN
                     QEGDYMVIMVFGEYQPEPIHLLMDFLKETFTEIKS"
     gene            955..>1402
                     /locus_tag="PK976_06755"
     CDS             955..>1402
                     /locus_tag="PK976_06755"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HRC67621.1"
                     /translation="MSIILGLGNSFCQAQTGGTKTYEFLNLPTSARAAALGYNVLSIS
                     DNDISLVNTNPSLLNPEMHNNLVLSFVDYFADINYGYATYARSFSKTGNFAATLQFID
                     YGTFTSADPTGSIQGSFGANELALNLSWGRSLDSNFSIGASLKGIYS"
BASE COUNT          414 a          310 c          262 g          416 t
ORIGIN      
        1 cgatttgatt tcggtaaaag tttcttttaa aaaatccatt aaaagatgaa taggttcagg
       61 ttgatattct ccaaagacca ttattaccat gtaatcgcct tcttgattat tacgaatgat
      121 gaggttgcgt aaaaatccgt ggtgggtaac cagatcctga aaaaccaaac catgttcgag
      181 ggcaaacctc ttcacggcca aacgaatctt attcgaggat tcaggctgaa gatagcaatg
      241 ctggatatcg agtacacggt cgaaccgatc aggtaaatgc aaacccaaag cctgcagggt
      301 aatttccgga tcatctgcat tttcgccctc atccaagaac cttcgattac tgaaggtaaa
      361 ctccatttta ttacgataag atacttttcc tggtgaaccg agaatgggaa gaggttctgg
      421 aaattcaaat ttacctaaac gcaacaaggt gtcattcacc catttggcct tatagaacaa
      481 ttgagtagaa tagtccatat tttgccagcg gcatccccca catataccaa aatggctgca
      541 ccaaggtttt ctcctgtttt cggattcctt gataattctt ataagacgag tttcccagaa
      601 tcgttttctt ttccttacta cccgaacatc gacaatatcg ccaggtacag cccatggaac
      661 aaatattacc atatcctgaa acctcccaac tgccatacct tcagcacctg catcggttat
      721 ctcaagccgt tcgataatgg gaagattcct attgcgcatt gctacaaaac ttcggcttac
      781 aaaattactt taaattcaac aacgggctcc atgtaataaa aaccactctc ctccgtttag
      841 gtgaaatcag acacggcaat tggttgtcat actgcttttg tttactttta cccgccgttt
      901 tataatatta aatttaatcc tgacacagtg agacatagaa aaaatatcct tgtattgagc
      961 ataattttag gcttggggaa ttctttctgc caggcgcaaa caggaggcac aaaaacttac
     1021 gaattcttga atttacccac atcggcgcgt gctgccgcct taggctataa tgtattgagt
     1081 atcagcgata acgacatttc tctggtcaat accaacccct cacttttgaa tccagaaatg
     1141 cacaataatt tggttttgag ctttgtggat tattttgcag atatcaatta tgggtatgcc
     1201 acctatgccc gttcattctc caaaaccgga aactttgcag ctaccctaca atttattgat
     1261 tatggcacct ttacctccgc cgaccctacg ggtagcattc aaggaagttt tggagcaaat
     1321 gagctggctt tgaacttaag ttggggtcga agtttggata gcaattttag tattggagca
     1381 agtttaaagg gaatttattc ct
//
LOCUS       DAORPD010000558         1398 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_18700_length_1398_cov_2.796724, whole genome shotgun sequence.
ACCESSION   DAORPD010000558 DAORPD010000000
VERSION     DAORPD010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18700_length_1398_cov_2.796724"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="PK976_06760"
     CDS             complement(<1..415)
                     /locus_tag="PK976_06760"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRC67622.1"
                     /translation="MKQRFFIIAFLLGFFFITSKAQEPAEVVVSQQKITENGMTFWLH
                     TVEAGQTLYRISLAYKVPISDILQYNPEAAKVLKTGQQLKIPATPSEISPYTNIDFIY
                     HVTKAGETLQSISNIYSVPIETIQKLNPGIGKDKLP"
     gene            complement(427..>1398)
                     /gene="guaA"
                     /locus_tag="PK976_06765"
     CDS             complement(427..>1398)
                     /gene="guaA"
                     /locus_tag="PK976_06765"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HRC67623.1"
                     /translation="ICHCSQDWTPASFVDETVQYLKNLIGNDEVIMGLSGGVDSTVAG
                     MLLHHAIGNHLHCIFIDNGLLRKNEFGHVLESYKRMHLNVKGVDASGRFLRALEGISD
                     PEAKRKIIGRTFIDTFDEEAAKLEGIKWLGQGTIYPDVIESVSVNGPSATIKSHHNVG
                     GLPDMMNLKIVEPLRNLFKDEVRLVGRELGIDKEFLSRHPFPGPGLGIRVIGEITRER
                     LEILREADAIFVDALKQAGLYDKVWQAFVVLLPVKAVGVMGDERTYENVLAIRSVQST
                     DGMTADWTHLPYDFLAQVSSSLINHVNGINRVVYDISSKPPATIEWE"
BASE COUNT          396 a          317 c          288 g          397 t
ORIGIN      
        1 caggaagttt gtctttacca ataccggggt tcaatttttg gatggtttcg atgggaacgc
       61 tataaatgtt cgatatactt tgcaaggttt cgcctgcttt ggttacatga tatataaaat
      121 caatattcgt gtaagggcta atttctgaag gcgtcgcagg aatcttcaat tgttgccctg
      181 ttttcaatac tttggctgct tcggggttat attgaagaat atcactaatt ggcaccttat
      241 aagcaagtga aatccggtaa agtgtttgac cggcttcgac ggtatgcagc cagaaagtca
      301 tgccattttc agtgattttc tgttgagata ccacaacctc agcaggttcc tgagcctttg
      361 aagtaatgaa aaagaatccc agaagaaaag cgataatgaa aaatctttgc ttcatgattt
      421 cgataattat tcccactcga tggtagcagg gggtttcgaa ctaatatcgt agaccacgcg
      481 attgatccca tttacgtgat tgattagaga gctcgagacc tgagccagaa aatcgtaggg
      541 aagatgcgtc cagtcggcgg tcataccgtc ggttgactgt actgaacgaa tagccagcac
      601 attttcgtat gttcgctcat ctcccattac gccaacggct tttacgggga gtaataccac
      661 aaaagcctgc caaactttgt cgtagagccc agcttgttta agcgcatcga cgaaaatggc
      721 atcggcttca cgtaaaatct caagcctctc acgggtaatt tcacctataa ctctaatacc
      781 cagtccaggc ccggggaaag gatggcgact cagaaattct ttatcaatgc caagctctct
      841 gcctaccaaa cgaacttcat ctttaaaaag gtttcgcaga ggttcgacta ttttcagatt
      901 catcatatcg ggaaggcctc caacgttatg atgcgactta atggtggccg aaggaccatt
      961 caccgaaacc gactcgatta catcgggata aatcgttcct tgtcctaacc attttattcc
     1021 ttctaactta gcggcttctt cgtcgaaagt gtctataaaa gtccttccaa tgattttgcg
     1081 ttttgcctca ggatcggaga ttccttcaag agctcgtaaa aatcttcccg aagcatctac
     1141 acctttaaca ttgagatgca tccgcttata ggattccaaa acatgaccaa actcattctt
     1201 tcgcaataat ccattatcga taaaaataca atgcagatga ttgcctattg catgatgtaa
     1261 aagcattccg gctactgtgg aatccactcc gcccgacaaa cccatgatga cttcatcatt
     1321 gccgataagg ttcttcaaat actgtacggt ttcatcgaca aaggacgccg gagtccaatc
     1381 ttgggagcaa tgacaaat
//
LOCUS       DAORPD010000559         1397 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_18721_length_1397_cov_2.605812, whole genome shotgun sequence.
ACCESSION   DAORPD010000559 DAORPD010000000
VERSION     DAORPD010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18721_length_1397_cov_2.605812"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="PK976_06770"
     CDS             complement(<1..356)
                     /locus_tag="PK976_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRC67624.1"
                     /translation="MSSIIESASYNEESIRSLDWREHIRMRPGMYIGKLGDGASHDDG
                     IYVLLKEIMDNAIDEFVMGFGKTIEVNIQDRRVNVRDYGRGIPLGRLLECVSKINTGA
                     KYDSKVFKKSVGLNGVG"
     gene            513..>1397
                     /locus_tag="PK976_06775"
     CDS             513..>1397
                     /locus_tag="PK976_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 92 protein"
                     /protein_id="HRC67625.1"
                     /translation="MKKFLLIINLLAFSWTFGQGNSKYVNPFIGTDKMGHTFPGATVP
                     FGFVQLSPDTDTIPYNDGNVYNPQVYQYCAGYQYNDPTIVGFSHTHLHGTGHSDLGDF
                     LLMPVSGKIKINPGIASKPGSGYRSRYDKSREKAEAGYYRVCLTDYDIDAELTATQRT
                     GVHRYTFHRADSCYLLLDMTHGIYNYDGKVVWSSLHVVNDTLVTGYRQTNGWARERFI
                     YFAMTFSKPVQEYNLLNLDKEIYTGFWRRWKMDHNFPERSGRKLKAIFKFDIRSGEQL
                     VVKMGISGVSEQGAMKNLL"
BASE COUNT          385 a          284 c          296 g          432 t
ORIGIN      
        1 ccaacaccgt tcagtcctac cgacttttta aaaaccttcg aatcatattt tgcgccggta
       61 ttgattttcg agacacattc aagcaggcgt cccaggggaa tacccctgcc atagtctctt
      121 acattcactc gccgatcctg aatgtttact tctatggttt tgccaaatcc catcacgaat
      181 tcatcaatgg cgttgtccat gatttctttc aggagaacat agatgccgtc gtcgtgtgaa
      241 gccccatcac ccagctttcc aatatacatt ccgggccgca tgcgaatatg ttcacgccag
      301 tcgagtgagc gaatactttc ttcattataa cttgctgatt ctataattga actcattatt
      361 tcatcccttt gttgcaaaga taaggctaat tcgcgcacgg aactatggat aaccgaaaag
      421 aattcgagat gcatttatgg aggaattcaa aacccttaga gacttttccg taatttcgtg
      481 ctgttttata tctaaacctt taaagatttt cgatgaaaaa atttttactc attatcaatt
      541 tattggcttt tagttggacc tttggtcagg gaaattcgaa atatgtgaat ccatttattg
      601 gaaccgacaa aatggggcat acttttccag gggctacggt tccatttgga tttgtgcagt
      661 tgagtcccga taccgataca attccttata atgatgggaa tgtctataat cctcaggttt
      721 atcagtattg tgcaggttat cagtataacg atccgaccat tgtggggttt agtcatactc
      781 atttgcatgg tacgggtcat tccgatttgg gcgatttttt attgatgcct gtttcaggca
      841 agataaaaat caatcccggt atagcttcaa aaccagggag tggctataga agccgatacg
      901 acaaaagtcg tgaaaaggcc gaagcgggtt attaccgggt ttgtcttacc gattatgaca
      961 tagatgcgga acttacggct acccagcgca ctggcgtaca tcgttacact tttcatcgtg
     1021 ccgacagctg ctacctttta ctcgatatga cgcatggaat ttataattac gacggcaaag
     1081 tcgtgtggag ctcattacat gtagtcaatg atacactggt tacgggttat cgtcaaacca
     1141 acggatgggc ccgtgagagg tttatctatt ttgccatgac attttctaaa cccgttcagg
     1201 aatataattt gctcaatctt gataaggaaa tctatacagg tttttggcgc agatggaaaa
     1261 tggaccataa ttttccagaa cgtagcggtc ggaaattaaa ggccattttt aagttcgata
     1321 ttcgatcggg tgaacaattg gttgtaaaaa tgggtatttc aggagtgagt gagcaaggtg
     1381 ccatgaaaaa tcttctt
//
LOCUS       DAORPD010000560         1397 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_18725_length_1397_cov_2.061848, whole genome shotgun sequence.
ACCESSION   DAORPD010000560 DAORPD010000000
VERSION     DAORPD010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18725_length_1397_cov_2.061848"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /gene="surE"
                     /locus_tag="PK976_06780"
     CDS             <1..687
                     /gene="surE"
                     /locus_tag="PK976_06780"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF001492.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HRC67626.1"
                     /translation="MGHVIVAAPDKPQSGMAHSITVNIPLRYQKISEEENYEEYGISG
                     TPVDCVKLAEKFLVPRKPDLLVSGINHGSNASINILYSGTMAAVLEGCMLDIPSIGFS
                     FCDYSYHANFSCLDEWVTRIARIILENGLPRGVCLNVNIPETGGEPIRGIHITHQGEG
                     RWEEEFDHRLDPRERDYFWIMGNFINYDGQPGSDLCALENNFISVMPVQYDMTAHYAL
                     DYLKKIFNNE"
     gene            680..970
                     /locus_tag="PK976_06785"
     CDS             680..970
                     /locus_tag="PK976_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67627.1"
                     /translation="MNKNTYWFGLLAGLLGMITGGILFWLIGLLLTAITGLDPFFQPW
                     QMYWLSLIIPIVLIRHFFMRRKFERTGRGVLTMVFVLVLGYFIYVRIKAGTI"
     gene            967..>1397
                     /locus_tag="PK976_06790"
     CDS             967..>1397
                     /locus_tag="PK976_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009011443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HRC67628.1"
                     /translation="MKYYLIVGEASGDLHASNLVKAIQELDSEAEFRGFGGDLMKKAG
                     VNIIKHHRDLSFMGFVEVAGHLPTIIKILNECKSDILNFQPHALILVDYPGFNLRIAK
                     FARKNNIKVIYYVSPQVWAWHQSRVRSIKKYVNRMITILPF"
BASE COUNT          404 a          274 c          299 g          420 t
ORIGIN      
        1 atgggtcatg ttattgtagc cgctcctgat aaacctcagt cggggatggc tcattccatc
       61 accgtaaata ttcctttgcg ttatcagaaa attagcgagg aagaaaatta tgaggaatac
      121 ggtataagtg gcacacctgt cgattgtgtg aaactggccg aaaaatttct cgtaccccgg
      181 aaacctgatc ttttggtttc gggcatcaat catggttcta atgcctctat caacattctg
      241 tattctggaa ctatggcagc tgtattggaa ggttgcatgt tagatattcc cagcataggt
      301 ttttcgtttt gtgattattc ataccatgcc aatttcagtt gcctcgatga atgggttacc
      361 agaattgcac gcattattct cgaaaatgga ttaccaagag gagtctgctt gaatgtaaac
      421 attcccgaaa caggaggtga accaatccgt ggaattcata ttacacatca gggtgaagga
      481 agatgggaag aagaattcga tcatcgcctc gatccacgcg aaagagatta tttctggata
      541 atggggaatt ttataaatta cgatgggcaa ccgggtagtg atctttgcgc cttggaaaac
      601 aacttcattt cggtaatgcc tgttcagtac gacatgacgg cacattatgc cttggattac
      661 ttgaagaaaa tattcaacaa tgaataagaa tacctattgg tttggacttt tagccggact
      721 gctcggaatg ataaccggag gaatactgtt ctggctcatc ggcttacttc ttactgcaat
      781 tacgggatta gatccatttt ttcaaccatg gcaaatgtat tggctttcac ttatcatccc
      841 aatcgttttg atccgacatt tttttatgcg acgaaaattc gaacgcacag gtagaggagt
      901 tttaaccatg gtctttgtcc ttgtattggg ctattttatt tatgtaagga taaaggcggg
      961 aactatatga aatactatct cattgtaggt gaagcctcgg gtgatttaca cgcttccaat
     1021 ctggtaaagg caatccaaga actcgacagc gaagccgaat tccgtggctt tggtggagat
     1081 ttgatgaaga aagctggggt aaacatcata aaacaccaca gagacctctc atttatgggt
     1141 tttgtcgaag tggccggtca tctccctacc attataaaaa ttctaaatga atgcaaatcc
     1201 gatatcttaa actttcaacc tcacgctctt attcttgtcg attatccggg ttttaatttg
     1261 aggatagcca aatttgcccg aaaaaataac atcaaagtca tctattatgt atcaccacag
     1321 gtttgggcat ggcatcaatc gcgggtacgt agcataaaga aatacgtcaa ccgcatgatt
     1381 actatacttc ctttcga
//
LOCUS       DAORPD010000561         1393 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_18808_length_1393_cov_2.940209, whole genome shotgun sequence.
ACCESSION   DAORPD010000561 DAORPD010000000
VERSION     DAORPD010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18808_length_1393_cov_2.940209"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1393)
                     /locus_tag="PK976_06795"
     CDS             complement(<1..>1393)
                     /locus_tag="PK976_06795"
                     /inference="COORDINATES: protein motif:HMM:NF028438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HRC67629.1"
                     /translation="KTNNYNFIYKQSVIKPALIGLIGAWISGGLEWNIPHHHRASTFL
                     PVQWSSEDNPDGSKTVWIGELEIRHRMRWAVGYTLRPGSSILECSVRIVNRTPVANSM
                     LCFANIAVHTNENYQVIFPPRTQWSTGHSKRSFTPWPVVNGLDVSWYKNNPRSASWFA
                     VNYEDDFVAGYDHGKNAGIMSVADHNIVPGKKFFTWGVGSMWDKILTDDDGPYLEIMV
                     GAYSDNQPDYSWMHPYEERAFKIYLYPFRGIEGAKNANHDAAVNLEVKDGKALFGFYT
                     TRSHSNAVVTLKAADKVLLEEKISINPENPYKKEISLPEGIDEHDLRASISAEGSELI
                     SYSPVRIIPEPMPMPYTDPKAPADIDNNEELFLAGLRIDQFHHPSLDADPYYLEVLRR
                     DSANVAANTEMGILSLKKARYAEAEKYLNMAIRRLTANHTTPKNAEPFYYLGLALKKQ
                     GRLDEAYTAFYKAA"
BASE COUNT          385 a          312 c          278 g          418 t
ORIGIN      
        1 atgcggcttt ataaaaagca gtataagctt catccagacg cccctgtttt ttaagagcta
       61 aaccgagata atagaatggc tcagcatttt ttggtgtagt atggttggct gtaaggcgtc
      121 ttatagccat attcagatat ttctccgcct cagcgtatct tgctttttta agactcaaaa
      181 ttcccatttc cgtatttgca gccacattgg ctgaatcgcg acgaagtact tccagataat
      241 acggatcggc gtcgagtgat ggatggtgaa actggtcaat gcgtaaaccg gcgagaaaga
      301 gttcttcatt attatcaatg tctgcaggcg cttttggatc agtgtaaggc attggcattg
      361 gctcgggtat tatgcgtacc ggagaatatg aaataagttc gctgccttca gccgatatag
      421 aagcacgaag atcatgctcg tcaatgccct cgggcaacga tatttctttt ttataaggat
      481 tctcaggatt gatagatatt ttctcctcga gaagaacttt atctgctgct ttaagagtaa
      541 caaccgcatt tgaatgcgac cttgttgtat aaaacccgaa taatgctttt ccatctttaa
      601 cctcgagatt tacagcagca tcatgattag catttttcgc accttcaatt cctctgaagg
      661 gatataagta tatcttaaaa gctctttcct cgtatgggtg catccatgaa taatctggct
      721 gattatcgga atatgcacct accattattt caaggtaagg gccgtcgtca tcggtaagaa
      781 ttttgtccca catagatcca acaccccaag taaagaattt tttccctggt acgatattat
      841 gatctgcaac actcattatg ccagcatttt tcccatggtc atagcctgcc acaaaatcgt
      901 cctcatagtt tactgcgaac cacgatgccg acctcgggtt gtttttatac caacttacat
      961 caagaccatt gacaacaggc catggcgtga aactacgttt actatgacct gtagaccatt
     1021 gtgtacgagg tggaaaaatt acttggtaat tttcgtttgt atgaacggca atatttgcaa
     1081 aacaaagcat cgaatttgcc acaggagtgc gattcacaat ccttactgag cactcgagta
     1141 tggaactgcc cggccggaga gtataaccta ccgcccatct catccgatgt cgaatctcaa
     1201 gttctccaat ccatactgtt ttacttccat cagggttatc ctcactgctc cattgaaccg
     1261 gaagaaatgt acttgcacgg tgatgatgcg gaatgttcca ttccagtcca ccggaaatcc
     1321 atgctccaat caacccgata agagcaggct tgataacact ttgcttgtaa ataaaattat
     1381 aattgttcgt ctt
//
LOCUS       DAORPD010000562         1390 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_18873_length_1390_cov_1.984270, whole genome shotgun sequence.
ACCESSION   DAORPD010000562 DAORPD010000000
VERSION     DAORPD010000562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18873_length_1390_cov_1.984270"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1157
                     /locus_tag="PK976_06800"
     CDS             <1..1157
                     /locus_tag="PK976_06800"
                     /inference="COORDINATES: protein motif:HMM:NF024896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRC67630.1"
                     /translation="PTLLECLENQSFQNFKLVVCVNQPDDYRFNPNKIHIYEDNQKCL
                     SLLKSSSYSFDIEIIDRSSTDMGWKGKHCGVGQARKTVMDAIAREAKNNDDIIVSLDA
                     DTLFGTDYLEVIAQTFLDYSYIAALSVPYYHPLTQDEITNRCILRYEIYMRVYALNML
                     FIHNPYAFTAIGSAMALRVDIYRKVGGITPHRAGEDFYFLQKLRKHGSVLTWCPAKIY
                     PAARFSDRVIFGTGPAMIRGREGHWEAYPIFHHSLFDTVKITFEAFSRLYLEKIKTPM
                     DVFLEDIFRTSDIWGPLRKNSSEIKSFVKACTQKIDALRILQFLKKYHPQIKKSDEIC
                     LKEFMMHYFPESHPALIESFDTCNINALQEIRDFLTMKEYSYRQHHPLLQ"
     gene            1180..>1390
                     /locus_tag="PK976_06805"
     CDS             1180..>1390
                     /locus_tag="PK976_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl transferase family protein"
                     /protein_id="HRC67631.1"
                     /translation="MITILGPTAVGKTRISAMIAARMDAEIISADSRQVFRGMDLGTG
                     KDLQDYFVDGSFIPYHLIDIRQPGEE"
BASE COUNT          397 a          265 c          288 g          440 t
ORIGIN      
        1 tgcctacttt gctcgaatgt ttggagaatc aaagtttcca gaatttcaag cttgtagttt
       61 gtgtaaatca acccgatgat tatcgcttca atcccaataa aatccacatt tatgaagaca
      121 atcagaaatg cttgagttta ctgaaatcca gttcatattc atttgatatc gagattatcg
      181 ataggtcttc taccgatatg gggtggaaag ggaagcattg tggggtaggg caagcccgca
      241 aaacagtgat ggatgctatt gcacgggaag ctaaaaataa cgatgatatc attgtgagtc
      301 tggatgctga tacgctattt gggacagatt atttagaggt tatagcccag acttttcttg
      361 attattctta tattgctgct ttatcggttc cttattatca tccgctcacc caagatgaaa
      421 ttaccaatcg ctgtattctc cgttatgaaa tatatatgcg ggtgtatgct ctgaatatgt
      481 tattcattca taatccctat gcttttacgg caattggttc ggctatggct ttaagagtcg
      541 atatttaccg aaaagtagga ggcattacac cgcatcgagc tggtgaagat ttctattttt
      601 tacaaaaact ccgtaaacat ggttcagtgc ttacgtggtg tccagcgaaa atctatcctg
      661 ctgcacggtt ttccgataga gttatctttg gcactggccc ggcaatgatt cgaggcaggg
      721 aaggccattg ggaggcctat cccatttttc accattcatt gttcgatacg gtgaaaatta
      781 ccttcgaggc attctcgaga ttatacttag aaaaaataaa aacgcctatg gatgtttttt
      841 tagaagatat ttttcgaact tccgacattt ggggaccact gcgtaaaaat tccagtgaga
      901 taaaatcgtt tgtaaaggct tgtacacaga aaattgatgc cttgcgcata cttcaatttc
      961 tgaaaaaata ccatcctcaa atcaaaaaat ctgacgagat ttgcctcaaa gaattcatga
     1021 tgcattattt ccctgagagt catccagcgc tgatcgaaag tttcgacact tgcaatataa
     1081 atgccttgca ggaaattcgt gattttctta ctatgaaaga atattcttat cgacagcatc
     1141 atcctttgct tcagtagttg cttttataca cttttataca tgattacaat acttggacct
     1201 acggcagttg ggaaaacacg catttcggct atgatagctg ctcggatgga tgccgagatt
     1261 atttcggccg attcacgtca agtgtttcgt ggaatggatt taggcacagg caaggattta
     1321 caggattatt tcgtagatgg cagttttatt ccctatcatt tgatcgacat ccgtcagcca
     1381 ggcgaagaat
//
LOCUS       DAORPD010000563         1389 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_18888_length_1389_cov_2.413043, whole genome shotgun sequence.
ACCESSION   DAORPD010000563 DAORPD010000000
VERSION     DAORPD010000563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18888_length_1389_cov_2.413043"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1362)
                     /locus_tag="PK976_06810"
     CDS             complement(<1..1362)
                     /locus_tag="PK976_06810"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HRC67632.1"
                     /translation="MGVIDFNRAKARFSISIHSSIPKLQPYSLIQWLGAYHPLLLLKN
                     QAQKKPVVPMDLILNEDQRILIISGPNAGGKSVCLRTAGLLQYMLQCGIPIPVKETST
                     AGIFWDIFLDIGDEQSIENDLSSYSSHLLNMKFFLKNATSQTLFLIDEMGEGTEPRVG
                     GAIAEAILEKLSQQKAFGVITTHYSNLKSLEGKVEGIVNGAMLFDIHRMQPLYRLQIG
                     KPGSSFAFEIAHILGFPEEVMQLAAEKTGETQLSFDHQILRIEDEKNQLEQKKAELQL
                     ADELLTRALKRYQDLYNQLDQSKKEILKKAREEAAELIRQSNRLIEKTIEEIKKSDAE
                     RKTTQKLRETLKEFQKVLEEESKELEPRPIHLVKQEINDNKESTNETKPLQVGDWVRI
                     KNLDASGELFSLNGQDAVVIINSVKLNIRADRLEPASKPVFEKKIRGHLLEDINQKLQ
                     DFRL"
BASE COUNT          362 a          276 c          288 g          463 t
ORIGIN      
        1 caatcgaaag tcttgaagtt tttggttgat atcttccaac agatggccac gtatcttttt
       61 ttcgaaaact ggtttcgaag caggttcaag acgatcggca cggatattga gttttactga
      121 attaataatt acaacagcat cctgaccatt caacgaaaag agttcccccg aggcatccag
      181 gtttttgatg cgtacccaat caccgacttg taatggcttt gtttcattcg tagattcttt
      241 attgtcattt atttcttgtt taacaagatg gataggtcga ggctcaagtt cttttgattc
      301 ttcttccaga actttctgga attctttgag tgtttctctt aatttttgcg tagttttcct
      361 ttcggcgtcc gattttttaa tttcctcgat ggttttctct attaaacggt tcgattgtct
      421 aattagttct gcggcttctt ctctggcttt tttaaggatt tcctttttcg actggtctaa
      481 ttgattatac aaatcctgat aacgtttaag ggctcgagtc agtaattcat cggcaagttg
      541 aagttcggct ttcttttgtt cgagttgatt tttctcatct tctattcgta aaatctgatg
      601 atcgaatgaa agctgagttt ctccggtctt ttcggctgcc aattgcatga cttcttcagg
      661 aaaccccaga atatgggcaa tctcgaaggc gaacgaggaa cccggtttgc caatctgtaa
      721 tcgataaaga ggttgcattc tatgaatgtc gaaaagcatt gcgccgttga ctatgccttc
      781 aactttccct tcaagcgatt tcaaattgga ataatgtgtg gtaataacgc caaaagcctt
      841 ttgctggctg agtttttcca atatggcttc ggcaatagcc cctcctactc ggggttcagt
      901 cccttcgccc atttcatcaa tcaagaatag agtttgtgaa gtagcatttt taaggaaaaa
      961 cttcatattc aataaatgag agctatagct gctgagatca ttttcgatgg attgttcgtc
     1021 tccgatgtcg aggaagatat cccaaaaaat tcctgctgtt gaagtttctt ttacgggtat
     1081 gggaatgccg cactgaagca tatattgaag caagcccgcc gtccgaagac ataccgattt
     1141 tccgcctgca ttagggcccg aaatgataag aatccgctgg tcctcattca ggataaggtc
     1201 cataggaact acaggttttt tttgagcctg attcttaagt agaagcaaag gatgataagc
     1261 tccgagccac tgtatcaatg aataaggttg gagcttggga atagaggaat ggatagaaat
     1321 cgagaaacgg gctttggccc tgttaaaatc aataactccc ataaatgtat atgcttctat
     1381 taaatcatc
//
LOCUS       DAORPD010000564         1389 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_18895_length_1389_cov_1.877811, whole genome shotgun sequence.
ACCESSION   DAORPD010000564 DAORPD010000000
VERSION     DAORPD010000564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18895_length_1389_cov_1.877811"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1389
                     /gene="secA"
                     /locus_tag="PK976_06815"
     CDS             <1..>1389
                     /gene="secA"
                     /locus_tag="PK976_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658948.1"
                     /note="functions in protein export; can interact with
                     acidic membrane phospholipids and the SecYEG protein
                     complex; binds to preproteins; binds to ATP and undergoes
                     a conformational change to promote membrane insertion of
                     SecA/bound preprotein; ATP hydrolysis appears to drive
                     release of the preprotein from SecA and deinsertion of
                     SecA from the membrane; additional proteins SecD/F/YajC
                     aid SecA recycling; exists in an equilibrium between
                     monomers and dimers; may possibly form higher order
                     oligomers; in some organisms, there are paralogous
                     proteins that have been found to be nonessential but do
                     function in secretion of a subset of exported proteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HRC67633.1"
                     /translation="LYFVIDEKNNTIDLTEKGIDALTESYQDPEFYLLPDVGSVIAEI
                     EKMNIPESEKEERKSELMRDYAIKSERVHTVNQLLRAYALFEKDVEYVIIDNKVKIVD
                     EQTGRILEGRRYSDGLHQAIEAKENVKVEAATQTYATITLQNYFRMYKKLAGMTGTAE
                     TEAGEFWDIYKLDVVVIPTNKPVIRTDREDMVYKTKREKYNSVIEEIENLIKIGRPVL
                     VGTTSVETSELLSRMLSLRKIKHNVLNAKQHAREAEIVAHAGLAGMITIATNMAGRGT
                     DIKLGPGVKEAGGLAIIGTERHESRRVDRQLRGRAGRQGDPGTSQFFVSLEDDLMRLF
                     GSERIARIMDRLGIQEGEVIQHPMITRSIERAQRKVEENNFGIRKRLLEYDDVMNAQR
                     EVIYSRRRNALHGERLSLDIRNMMEDVCEHLIEEYTDPRDYQGFRMELLRIFGMDSPV
                     NEDEFHSKSEKE"
BASE COUNT          443 a          310 c          326 g          310 t
ORIGIN      
        1 agctttattt tgtaattgat gaaaaaaaca ataccatcga ccttactgaa aagggaatcg
       61 acgctttgac tgaatcttat caggaccctg aattctacct ccttcccgac gtgggctcgg
      121 taattgcaga aatcgagaaa atgaacattc cagaatcgga aaaagaagag cgcaagagcg
      181 aacttatgag ggactacgcc atcaagtcag aacgcgttca tacagtaaat caattattga
      241 gagcttatgc acttttcgaa aaagacgtgg aatatgttat catcgataat aaagtaaaaa
      301 tcgttgacga gcaaacggga cgtatcctcg aaggccgacg ttattccgac ggtcttcatc
      361 aagctatcga agccaaagaa aatgtgaaag tggaagcagc tactcaaacc tacgctacca
      421 tcactcttca gaattacttc cgcatgtaca agaaacttgc gggtatgacg ggtacagccg
      481 aaacggaggc cggcgaattc tgggacatct acaaactcga tgtagtagtc attccaacaa
      541 ataagcctgt catccgtact gatagggaag acatggttta caagaccaaa cgtgaaaaat
      601 ataactcagt catcgaagag atcgagaatt taatcaagat aggacggcct gttttggtgg
      661 gtactacttc ggtagaaact tccgaattgc ttagcaggat gttatcgttg cgtaaaataa
      721 agcataatgt acttaatgcc aagcaacatg cccgagaagc cgaaatcgta gcacatgccg
      781 gtttggccgg aatgattacc atagcaacaa acatggctgg tcgtggtacg gatataaaat
      841 taggaccggg cgtaaaggaa gcaggaggtt tagcaatcat tggtacggaa cgtcacgaat
      901 caaggcgtgt tgaccgtcag cttcggggac gggctggccg tcagggtgac cctggcacct
      961 cccaattctt cgtctcgctc gaagacgacc tgatgcgcct cttcggctcg gaacgcattg
     1021 cgcgtatcat ggaccgcctt ggtatccagg aaggagaagt gattcaacat cctatgatta
     1081 cccgctccat cgaacgtgca caacgtaaag tagaggaaaa taacttcgga attcgtaaac
     1141 gtctgctaga atacgacgac gtaatgaacg cccagagaga agtgatctac agcagacgcc
     1201 gcaatgccct ccatggcgaa cgcctttcac tcgatatccg caacatgatg gaagatgtat
     1261 gtgaacatct catagaagaa tataccgacc cccgcgacta tcaaggcttc cgtatggaac
     1321 tccttagaat attcggcatg gatagcccgg taaacgaaga tgaatttcat agcaaatcag
     1381 aaaaagaac
//
LOCUS       DAORPD010000565         1387 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_18936_length_1387_cov_2.963213, whole genome shotgun sequence.
ACCESSION   DAORPD010000565 DAORPD010000000
VERSION     DAORPD010000565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18936_length_1387_cov_2.963213"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="PK976_06820"
     CDS             complement(<1..686)
                     /locus_tag="PK976_06820"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HRC67634.1"
                     /translation="MKGQDPTFSQFYANPLYLNPSLTGSAFSPRLTLNYRNQWPSLAD
                     NFVTYNASYDQYVPDINSSFGIIFNTDRAGSGMITTTTVSGLYSYNLKISRNSYINAG
                     LQGSYYQRKLDWLNLTFGDMFHPVYGQIYPTSEPSPDKYSISFLDFSAGLTYTYRDVF
                     FGGLSVNHLTQPYDGWYNDNSTVLPRKWTLHAGGIFDLEPRTRGRRSMEAMTLSPNVL
                     YQQQQNFKQL"
     gene            1024..>1387
                     /locus_tag="PK976_06825"
     CDS             1024..>1387
                     /locus_tag="PK976_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67635.1"
                     /translation="MTLRFNLLIFVCVCGLIFESFAQTSSSSQNITLKWDNYSLKDTL
                     SGNQRTILYFHDATNLYETGGLPIFIQRIPLKQLSDSIFVKIFPLISGEDNSTDVVSS
                     EFLNNEYQFFIKKLEINGK"
BASE COUNT          432 a          260 c          274 g          421 t
ORIGIN      
        1 ttgagctgtt tgaaattttg ttgctgttgg tacagaacat taggtgaaag cgtcatggct
       61 tccatacttc ttcggccgcg ggtgcggggt tcaagatcga agattccccc ggcatgaagc
      121 gtccatttgc ggggtaatac agtactgttg tcgttatacc agccgtcgta aggttgtgta
      181 aggtgattta ccgataagcc accaaaaaat acgtctcggt aggtataggt caaacctgca
      241 gaaaaatcga gaaaggaaat gctgtattta tcaggtgagg gctcacttgt gggatatatc
      301 tggccatata ccggatgaaa catatctcca aatgtcagat tcaaccaatc gagcttgcgc
      361 tgatagtaac tcccctgtaa gcctgcatta atgtatgaat ttcgcgatat tttcaggttg
      421 tacgaataaa ggccactcac cgttgtagtg gttatcatgc ccgatccagc tctgtcggta
      481 ttgaaaataa taccgaagct gctattgatg tcgggaacat attgatcgta agaggcatta
      541 taggttacaa aattatcagc caaagatggc cactgattcc gataattcaa agttagcctt
      601 ggactgaagg ccgaaccagt caaagaggga ttgagataca aaggattggc ataaaattgt
      661 gaaaacgtcg gatcttggcc cttcatgtca atacctatta tactgagaac cagaatcatg
      721 ctaaagatgt agcttttcct tctcataatc tttatgatat aaaaagtgat ttcttcggta
      781 cacaaaacga tccaacaata ttacgaattt tttttataag catcatttgt taaaaaaaat
      841 tcaaactttt tttcaacaat aaggctctgt aaagaacgtt aaaaaacctg aatagtatcc
      901 ctactggaaa gatttttaga gaagaatttc tttcgatcga taaaattttg aggtggaaat
      961 gcacaatttt gtctcgattt tgtttaaact ttcgaaggga tactttccca aacttatttg
     1021 cccatgacat taagattcaa cctattgatt tttgtttgtg tgtgtggact cattttcgaa
     1081 agtttcgccc aaacttcaag ttcgtcccaa aatatcacgc tgaaatggga taattatagt
     1141 ctgaaagata ctctttcggg aaatcaaaga accatcctgt attttcatga tgctactaac
     1201 ctttacgaaa ccggaggcct ccccatattc attcaaagga ttcctttgaa gcaattatcc
     1261 gacagcattt tcgtgaagat attcccttta atttcgggag aagacaactc cactgatgtc
     1321 gtctcgtcag agtttctaaa caatgaatat caattcttta taaaaaaatt agaaatcaat
     1381 ggaaagg
//
LOCUS       DAORPD010000566         1385 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_18992_length_1385_cov_1.868421, whole genome shotgun sequence.
ACCESSION   DAORPD010000566 DAORPD010000000
VERSION     DAORPD010000566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18992_length_1385_cov_1.868421"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1385)
                     /locus_tag="PK976_06830"
     CDS             complement(<1..>1385)
                     /locus_tag="PK976_06830"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HRC67636.1"
                     /translation="VNSRGTIYINRMISPKKIYYPRPFSKPDLPVIPMNIFQTSFPQY
                     PFQNEDEFDQWEKGEVVLSQGYDTKKDSILRINELGNWPSGVYRVSIYAIDTFGQPVE
                     NQRYFVLYDTHEKKPPYPTPLWFVAEQNSYEPGQTARFIIGSSEKNAKIFLQVVLPDS
                     VIMEDWIKLSNQQKLVEIPVKERYRGNFKINLLFFKNNHVFYESQTIEVPYSNKKIEF
                     TLSTFRSTLEPGAEETWQISLKEPSNKPVLAEVLANMYDASLDVFQNSAWNIPSYSPN
                     SIFSWWRIFNQNSVGGYYLNSNIGPYLPVKEIKFDRIDWFGYPVYARYKMYRGAVSAG
                     KTQQPNYELVVTQNAEELEADEGVMVYNKIYDDRTPKKVSIRKDFSETAFFMPHLTTD
                     SNGRLQLKFKMPESLTRWKFRLFAHTQDMKLGFIEKEIITAKQLMVVSNAPRFLREGD
                     TLIFPTKLTNM"
BASE COUNT          390 a          271 c          266 g          458 t
ORIGIN      
        1 catgttggtg agttttgtcg ggaaaatcag tgtatcgcct tcacgcaaga atcgaggagc
       61 attcgatacg accataagct gtttggcggt aataatttct ttctcgatga atccaagttt
      121 catgtcttgc gtatgagcaa acagccggaa tttccatctc gtgagcgatt ctggcatctt
      181 aaatttcaac tgtaagcgtc cattgctatc agtggtcaaa tgcggcatga aaaaggcggt
      241 ttcgctgaaa tccttacgaa ttgaaacttt ttttggtgtt ctatcatcat atattttgtt
      301 atagaccatc actccttcat cagcttctaa ctcctcagca ttttgtgtta ccacaagctc
      361 gtagtttggt tgttgtgttt ttccagccga aactgcacct ctatacattt tgtatcgtgc
      421 ataaactgga taaccaaacc aatctatcct gtcgaatttt atttctttaa caggtaagta
      481 tggcccaatg ttgctattca gataatatcc ccctacgcta ttttgattaa aaattctcca
      541 ccagctaaaa atagaatttg gggaataaga gggaatattc caggctgaat tttggaatac
      601 gtcgagggag gcatcataca tattggccaa cacttccgct aatactggct tgtttgaagg
      661 ttctttcagg ctaatttgcc aagtctcttc ggcaccgggt tccaacgtcg aacgaaaggt
      721 gctgagcgta aattcgattt tcttgttcga ataaggaact tcaatggttt ggctctcata
      781 aaaaacatga ttgttcttga aaaataagag attgatctta aaattccctc tatatctttc
      841 ttttacaggt atttcaacca acttttgctg attgcttagt ttgatccaat cttccatgat
      901 cacactgtca gggagtacaa cctgaagaaa aattttcgca tttttttcgg atgaacctat
      961 tatgaatcga gccgtttgtc ctggttcgta agagttttgt tcagcaacaa accacaaagg
     1021 tgtaggataa ggtggctttt tttcgtgagt gtcatataaa acaaagtacc tctgattttc
     1081 aactggctgg ccaaatgtat caatggcata aatactgacc ctgtaaacac ctgaaggcca
     1141 attccccaat tcatttattc ttagaatgct atctttcttt gtatcataac cttgtgatag
     1201 cacaacttct cctttttccc attgatcaaa ttcatcttca ttctgaaaag gatattgagg
     1261 aaaagatgtt tggaatatat tcatgggaat aacaggtagg tcgggtttac taaaaggtcg
     1321 gggataatag attttcttgg gagaaatcat ccgattgata taaatagttc cgcgcgaatt
     1381 caccg
//
LOCUS       DAORPD010000567         1384 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_19010_length_1384_cov_2.177577, whole genome shotgun sequence.
ACCESSION   DAORPD010000567 DAORPD010000000
VERSION     DAORPD010000567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19010_length_1384_cov_2.177577"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="PK976_06835"
     CDS             complement(<1..705)
                     /locus_tag="PK976_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67637.1"
                     /translation="MNAMKSFLLENHRIIALIALASSWFLIFPSCQRNIQQDEDSGFR
                     LDTIASPQYAGLFVAGYIHHHRAVWLKDPWDTTKIYSRFIILSHRLPNDSIPHGYQPI
                     ILPVNSIATTSSTLISHLSSLNILDHLIGVADTSYIFHPYIRQRIHDRQVQEIARGDR
                     IEAESLIACRPDLSFITLYAGQDYSRFQQMGLQIVPLADYMENHPLGRAEWIRFVGYF
                     VGKEKEADSIFRQIASE"
     gene            complement(677..1051)
                     /locus_tag="PK976_06840"
     CDS             complement(677..1051)
                     /locus_tag="PK976_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES family protein"
                     /protein_id="HRC67638.1"
                     /translation="MKPEKLKNIILIGDRVLIKAKSANSKTKGGLVLPPGYSEREEVQ
                     SGYVIKTGPGYPIPLPSESDIEPWKSKDEAPSYIPLQAKPGDLALFLQKGAIEIVIDS
                     EKYFIVSHQSLLLLERDEELFT"
     gene            1193..>1384
                     /locus_tag="PK976_06845"
     CDS             1193..>1384
                     /locus_tag="PK976_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67639.1"
                     /translation="MAFLYRPKPKKYDHKPIYYNPDEEKAKRWQEKKESSDPTSDKLR
                     RDIERQWALRQKKKKSGNIS"
BASE COUNT          454 a          264 c          284 g          382 t
ORIGIN      
        1 ctcagaggca atttgacgaa agatgctgtc ggcttccttt tcctttccca cgaaatatcc
       61 gacaaaacga atccattcgg ctcttcctaa ggggtgattt tccatataat cggctaaagg
      121 cacgatttga agccccattt gttgaaaacg actgtaatcc tgaccagcat acagcgtaat
      181 aaaagacaaa tcaggtcggc aggcaatcag tgattcggct tcgatacgat cgcctcgagc
      241 aatttcttga acttgtctat catggatacg ttgccttata taaggatgaa agatataaga
      301 agtatccgct acaccgatga gatggtccaa aatattcaga gaagaaagat gagagatgag
      361 ggtggatgaa gttgtagcaa ttgagttcac agggagaata atgggctgat atccatgagg
      421 aatggaatcg tttggcaaac gatgggacaa aatgatgaat ctggaataaa ttttcgtagt
      481 gtcccaggga tctttcaacc acacagccct atgatgatga atataaccag caacaaacaa
      541 tccagcatat tggggcgaag caatggtatc gagtcgaaaa cccgaatctt catcctgctg
      601 aatgtttctt tggcaggaag gaaaaattag aaaccatgat gaagctaagg caattaaagc
      661 aataatgcga tggttttcaa gtaaaaagct cttcatcgcg ttcaagcagt aacaaggatt
      721 gatgagaaac gataaaatat ttttcgctgt cgattacgat ttcgatagca cccttttgaa
      781 ggaacagggc taaatcgccg ggtttagcct gaagtggaat ataagatgga gcttcatctt
      841 tcgatttcca tggttctata tcactttcgc tgggtagagg aataggataa cctggcccgg
      901 ttttaatcac ataacccgat tgcacttctt ccctttcaga ataacccgga ggaagaacaa
      961 gaccaccttt agttttgcta tttgcactct tggcttttat caacacccta tcgccaatta
     1021 gaataatgtt ttttaatttt tcaggcttca ttgaatttgc ttgaaatgaa ttttatggca
     1081 aaattactat ttcctcccta agttcgtttt taatgtttaa ttcgaaccca aataaacgca
     1141 aatgaagtaa ttttgcaaaa tcgataacaa atttcaatca ttccgtaaca atatggcttt
     1201 tctgtatcgt cccaaaccca agaaatacga tcataaacct atttattaca atcctgatga
     1261 agaaaaggcg aagagatggc aggagaaaaa agagtcttcc gatcctactt ctgataaatt
     1321 gcgccgggat atagaaaggc aatgggcttt aagacaaaag aagaaaaaat cgggcaatat
     1381 ttcc
//
LOCUS       DAORPD010000568         1381 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_19081_length_1381_cov_2.395928, whole genome shotgun sequence.
ACCESSION   DAORPD010000568 DAORPD010000000
VERSION     DAORPD010000568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19081_length_1381_cov_2.395928"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="PK976_06850"
     CDS             complement(<1..192)
                     /locus_tag="PK976_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRC67640.1"
                     /translation="MNALILAAGRGTRLYPLTLRRPKALIEVGGISLLERAILKLSNE
                     GFQRIIINVHHFGDQIIAYL"
     gene            complement(264..>1381)
                     /locus_tag="PK976_06855"
     CDS             complement(264..>1381)
                     /locus_tag="PK976_06855"
                     /inference="COORDINATES: protein motif:HMM:NF015620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HRC67641.1"
                     /translation="EQLTEIYRQTLDALIRFQLAGKGFDFSKAYPRGCFDEQSILWDL
                     QYFKYYFLKLAGIFFDEQALETDFRSFAEFLTQAPSGYFMYRDFQSRNVMLHDDTLYF
                     IDYQGGREGPCSYDLASLLYDAKADIPHPIREILLRYYIEQMKQADSLDPEEFLSYWP
                     GFVYIRILQALGTYGYRGFFQQKTHFLLSIPYALNNLSYVLENYGFAKQMPELERVVR
                     VIANDQTFRSFGKQTQRLLVQIVSFSYRKGIPLDSSGNGGGFVFDCRALPNPGIYQQF
                     SKFTGNHTEVIEFFDKFPEIKEFIQNCTALIQQSIHSYLEKGYTQLLVCFGCTGGQHR
                     SVYCANALAEKISASNDYDVVLTHRELSFDKAIFMDE"
BASE COUNT          455 a          272 c          261 g          393 t
ORIGIN      
        1 caaatatgca attatttgat cgccaaagtg atgaacattg ataataattc tttgaaaacc
       61 ttcattcgat aattttagga tagccctttc gagcagactt attccgccca cttctatcaa
      121 agccttggga cgacgaagtg ttaaaggata aaggcgtgtt cctcttccgg ctgcaagaat
      181 caaggcattc attatattga actcgattag aaaattttaa aatatttggt tttttattca
      241 ctcatttcat taatagaaca aatttactca tccataaaaa ttgccttatc aaaagaaagt
      301 tcacgatggg tgagtacaac atcgtaatca ttagaagccg aaattttttc ggccaatgca
      361 ttggcacaat aaaccgaacg atgctgaccg cctgtacatc caaaacaaac caacagctgg
      421 gtataaccct tttcgagata ggaatgaatg ctttgctgaa tcagtgcagt acaattctga
      481 ataaactctt ttatctcagg aaacttatcg aaaaattcga tcacttcggt atgatttcct
      541 gtaaatttcg aaaattgctg atatataccc ggattaggca aggctctgca atcgaaaaca
      601 aaaccaccac cattgcctga ggagtcgagt ggaatacctt ttctgtacga aaaacttacg
      661 atttgtacca gcaatcgttg agtttgtttg ccaaaagatc tgaatgtttg atcattagca
      721 attactctta ccactctttc cagttctggc atttgcttgg caaagccata gttttcgagg
      781 acataggaca gattgttcaa ggcataaggt atgctgagga ggaaatgagt tttttgctgg
      841 aaaaatcccc gataaccata agtccccaat gcctgaagga ttctgatata cacaaagcca
      901 ggccagtagc taagaaattc ttcaggatca agagaatcag cttgtttcat ctgctctatg
      961 taataacgaa gtagaatttc acggatagga tggggtatat cggctttggc atcataaaga
     1021 agtgaagcca gatcatatga gcaaggacct tcccttccac cttgataatc tataaaatat
     1081 aatgtatcat catgaagcat gacgtttcgc gactggaaat cgcggtacat gaaatatccc
     1141 gaaggagcct gagtcaaaaa ctcagcaaaa gatctaaaat ctgtttcgag tgcctgctcg
     1201 tcgaaaaaaa ttccagcaag tttcaaaaaa taatatttga aatactgcaa atcccataag
     1261 atactttgct catcgaagca gccacgtgga taggctttcg aaaaatcgaa acctttacct
     1321 gcaagctgaa atcgaatgag agcatctaag gtttgccggt aaatttcggt aagttgttca
     1381 t
//
LOCUS       DAORPD010000569         1380 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_19119_length_1380_cov_2.132830, whole genome shotgun sequence.
ACCESSION   DAORPD010000569 DAORPD010000000
VERSION     DAORPD010000569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19119_length_1380_cov_2.132830"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="PK976_06860"
     CDS             complement(<1..280)
                     /locus_tag="PK976_06860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRC67642.1"
                     /translation="MDKLSFLILIIPCIYALMMIAFAFGWLWLPAYRNECRKSVSIII
                     AVRNEEENIPQLLQGLHLQSYPTHLLQIIIVDDHSTDRTFQLIENDPLV"
     gene            complement(287..1312)
                     /locus_tag="PK976_06865"
     CDS             complement(287..1312)
                     /locus_tag="PK976_06865"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HRC67643.1"
                     /translation="MTKVTKRNINIFLQILILIGAYGYLAWKLFIAPSTRLTAQNISQ
                     LFHISNFWLVISIVLLLMLVNWSIEAAKWKYLISKIEEVSFPLALKAIFTGVTVSIFT
                     PNRVGEFFGRVFLLKNRQPMRGILITILGSFSQIVIYMICGITGGIIMFYLFLLPQNP
                     SLTKLQWIIISLAVIILAVTILIFLNISFLGSILQNLIPKQWHRIKRWFAILSYYSKR
                     ELTKVLIMSFLRYCVFSFQYIILLRMFNVPLSVGNLLVALMVIFALLVIIPSVALSEL
                     GVRSTVAVFVIGMMLPVGLRNDPMVNLGIIGAASTLWLINVALPAIIGSFFVFKLKFF
                     KGNGPKS"
BASE COUNT          485 a          247 c          280 g          368 t
ORIGIN      
        1 gaaccaaagg gtcattctcg atcaattgaa aggtacgatc tgtagaatgg tcatcaacaa
       61 tgatgatttg aaggagatga gtaggatacg attgcaaatg gaggccttgc agaagttgag
      121 gaatgttttc ttcttcattt cttacagcga taatgatgct tacacttttt cgacattcgt
      181 tccgataagc cggaagccac aaccacccaa aagcaaaggc aatcatcatc agggcataaa
      241 tgcaaggaat aatcaagatc aagaatgata acttatccat attgccttat gatttgggac
      301 cattgccttt gaagaatttg agtttgaaca cgaaaaagct gcctatgata gcaggcaagg
      361 ctacattgat aagccataaa gtgctggctg cacctattat ccccagattc accatgggat
      421 cgttgcgaag gcctacaggc aacatcatac caataacaaa aacggctacc gtacttctta
      481 ctcccaattc ggaaagcgca acagaaggaa tgatgactaa taaggcaaag ataaccataa
      541 gtgccacgag taagttcccc acgctcaaag ggacattgaa cattcgaagt aaaataatat
      601 actggaaaga aaagacgcaa taacgtagaa agctcatgat gagaaccttg gtcaattctc
      661 ttttggaata atacgatagt atagcaaacc agcgttttat tctgtgccat tgtttgggaa
      721 tgaggttctg aagaatactt cccaaaaatg aaatattcag gaagatgagt atggttacgg
      781 ccaagataat aaccgctaac gatataatga tccattgaag ttttgttaga gaaggatttt
      841 gaggcagcag gaaaagataa aacatgatga ttccacctgt aatgccgcaa atcatgtaaa
      901 ttacgatttg agaaaaactt cctaaaatag taatgagaat gcctctcatg ggttgtctat
      961 tcttaagaag gaaaactctt ccaaaaaatt ctcctacacg gttaggagta aaaatactta
     1021 cggtaactcc cgtaaagata gctttcaaag ctaggggaaa gcttacttct tcgattttcg
     1081 aaattaaata tttccattta gcggcttcta tgctccaatt gacgagcatg agcaacaaga
     1141 cgatactgat gaccagccag aaattcgaga tatgaaaaag ttgagaaata ttttgtgcag
     1201 tgagacgagt agaaggagct ataaataatt tccacgccaa ataaccataa gctcctatca
     1261 ggattaaaat ctgaagaaaa atgttgatgt ttcttttggt tacttttgtc attactaaag
     1321 tttgaaattt tgacagggca agatagaatc attttaggga tagacccggg tactactatt
//
LOCUS       DAORPD010000570         1378 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_19160_length_1378_cov_2.718065, whole genome shotgun sequence.
ACCESSION   DAORPD010000570 DAORPD010000000
VERSION     DAORPD010000570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19160_length_1378_cov_2.718065"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(280..>1378)
                     /locus_tag="PK976_06870"
     CDS             complement(280..>1378)
                     /locus_tag="PK976_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="decatenates newly replicated chromosomal DNA and
                     relaxes positive and negative DNA supercoiling; Derived by
                     automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HRC67644.1"
                     /translation="FMVTRVAEKVFVGQNVVHINVFRRNDERTIYNMIYQDGKTGPIY
                     VKRFAVVSITRDKEYNLTPGKPGTKVLYFTANPNGEAEIVKLFLKPKPKLKKLQFEFD
                     FASLSIKGRNSKGNILTHHLVKSVIKKEAGVSTLGARNIWWDDTVNRLNVEGRGMLLG
                     AFMPEDRIITFTQSGYYRITGNDISAHFDDDLIYIAKFDEEKIITAVYRNENDGFFYI
                     KRFRPEPSDKKISFLGEEANHQLVAFSIDYLPQLKVEFEQTNRRKPREPLILNVSEFI
                     DIKSYKSKGKRISTHSIHSAVFIEPLPYIAEGIEEAEMILAEANDELIPNGNNGDEGL
                     ENNSPTSDTSTSDSFDKPQPMPKGIQLDLDF"
BASE COUNT          383 a          243 c          298 g          454 t
ORIGIN      
        1 ggaaagaatt tgtgttcccc gaaagttgaa attcaaaagg tcaacttgct gtataaaagg
       61 aagttacaac cctaaggcgc tcaagttggg ttaagtctgt gactaaataa ctgaaatata
      121 attgtttata atggatgggc gcaagggtta ataattctcc ctcattagcc attcgtgaaa
      181 tgaatggttc gaataaattt ggaatggatt gcattttata ctcttacaat aacagtaaag
      241 agcgatttga ttaagaatat aaagaagggg aatttatatt taaaaatcca gatcgagctg
      301 tataccttta ggcatgggct gaggtttgtc gaaagagtca gaggtggaag tatcactggt
      361 gggcgaattg ttttctaaac cttcgtctcc attgtttcca tttggaatga gttcgtcgtt
      421 ggcttcggca agtatcattt cggcttcttc gatgccttcg gcaatataag gtaaaggttc
      481 gataaagacg gccgaatgga tggaatgagt cgaaattctt tttcctttcg atttataact
      541 ttttatatca ataaactcgg aaacgttcag aataaggggt tctctgggtt tacgcctgtt
      601 ggtttgctcg aattcgactt ttagttgagg caggtaatct attgaaaatg ccacgagttg
      661 gtggttggct tcttcaccga ggaatgagat ttttttgtcg gaaggttcgg gtctaaagcg
      721 tttgatgtaa aaaaatccat cattctcatt tctgtaaaca gcagtgatta ttttttcttc
      781 atcgaatttt gcaatataga tcaggtcatc atcaaaatgt gcggaaatgt cattcccggt
      841 aatgcggtaa tacccgcttt gggtaaaagt aatgatcctg tcttcgggca taaatgcacc
      901 cagaagcatt cccctgcctt cgacattgag acgattcacc gtatcgtccc accaaatatt
      961 tcgtgccccc agagtcgaaa cacctgcttc ttttttaata accgatttta ccagatgatg
     1021 ggtaagtata ttgcctttac tgtttcgtcc tttaattgat agtgaagcaa aatcgaattc
     1081 aaactgaagc ttcttgagtt tgggtttagg tttcaggaat aattttacga tttcggcttc
     1141 tccattggga ttggcagtaa aatagagcac tttggtgcct ggttttcccg gtgtaaggtt
     1201 atattcctta tctcttgtta tgcttactac agcaaagcgt ttcacataaa tgggacctgt
     1261 ctttccatct tgatagatca tattgtaaat ggttctttcg tcgttgcggc gaaatacatt
     1321 gatatggacc acattttggc caacgaaaac tttttcggct acacgtgtaa ccataaag
//
LOCUS       DAORPD010000571         1378 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_19169_length_1378_cov_1.984883, whole genome shotgun sequence.
ACCESSION   DAORPD010000571 DAORPD010000000
VERSION     DAORPD010000571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19169_length_1378_cov_1.984883"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="PK976_06875"
     CDS             <1..541
                     /locus_tag="PK976_06875"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HRC67645.1"
                     /translation="YLEGLAAHWEILLFIIGLILIAIEIFAIPGFGVTGISGIILVVA
                     GLTLSLVGNFNAIPAGQIVQDILKAFFTVIIAIFISLISSFYGSKRLFSHPVPFLGSL
                     ALDTTQDKELGYTIANEDYSSMVGKTGFALTILRPAGKIMIEEEVFDAVAINGYISKG
                     QEVEVVRYENAQLYVKVKN"
     gene            553..1086
                     /locus_tag="PK976_06880"
     CDS             553..1086
                     /locus_tag="PK976_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018027668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HRC67646.1"
                     /translation="MENLEKIKERIEVAQGDITRCEVDAIVNAANTSLRGGGGVDGAI
                     HRAAGPLLLEACKKLDGCPTGDAVITPGFNLKARWVIHTVGPVWRGGKHDEAHLLASC
                     YRKCLSLSFENKFGQVAFPNISTGIYGFPKKDAAQIAIHTVIQFLPSHEFPQKVIFVC
                     YDNENYSIYQEILSSLF"
BASE COUNT          400 a          277 c          306 g          395 t
ORIGIN      
        1 atacctggaa ggattggcag cacattggga aatcttgctt ttcattatcg gtcttatctt
       61 gattgctatc gagatatttg ccattcccgg cttcggagtg acgggaatta gcgggattat
      121 tctcgtggta gccggtctta cactcagcct cgtgggaaat ttcaatgcca tacctgccgg
      181 ccaaatcgtt caggatattc ttaaggcatt ctttacggta atcatagcca tttttatttc
      241 gttgatctct tcattctatg gcagtaagcg cctcttcagt catcctgtcc ctttcctggg
      301 aagtctggcc ctcgatacta ctcaggacaa agaactcgga tataccattg ccaatgagga
      361 ttattccagc atggttggga aaaccggttt tgcacttacc atacttcgac ctgcaggtaa
      421 aatcatgatc gaagaagaag tattcgatgc tgttgctata aatggatata tctcgaaggg
      481 acaggaagtg gaggtggtgc gttacgaaaa tgctcagctt tatgttaaag ttaaaaacta
      541 atgattttaa gcatggaaaa tttagaaaaa ataaaagaaa ggatagaggt cgcacaggga
      601 gatattactc gatgtgaggt agatgccatc gtcaatgcag ccaatacttc cttgagaggt
      661 ggaggcggag tagatggagc aattcatcga gccgcaggcc ctctcttgct cgaggcttgc
      721 aagaaactgg acggttgtcc gacaggtgat gcagtaatca caccaggttt taacttaaag
      781 gcaaggtggg taatacatac cgtaggccca gtttggagag gtggtaagca cgacgaagct
      841 catcttttag catcatgtta tcggaaatgt ttaagcctga gtttcgaaaa caaattcggg
      901 caggtggctt tccccaatat cagtacgggc atttatggct tccccaaaaa agatgcagct
      961 caaattgcca tccatacagt tatccaattc ttaccatctc atgagtttcc tcaaaaggtc
     1021 atcttcgtgt gttatgataa cgaaaattat tcgatctatc aagaaatatt gtcctcactc
     1081 ttttaaatac ctatctccat ggctttttta gaggatttag cgggattcca tgcatcaaga
     1141 taggatgatg ctccataaat caataaatca gttttttgca tacacaacat gaggcaaaaa
     1201 ctttatttta cttatggata cgacttctga tcttattttt ctaaaatttt ggaaggaaga
     1261 cgagttaaaa aacggaaacg ctaagcatga tgcaataaaa aaaggccttt cggccatttt
     1321 ttgggtggac ggtgggattc gaacccacga catccggaac cacaatccga cgctctaa
//
LOCUS       DAORPD010000572         1378 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_19172_length_1378_cov_1.788360, whole genome shotgun sequence.
ACCESSION   DAORPD010000572 DAORPD010000000
VERSION     DAORPD010000572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19172_length_1378_cov_1.788360"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1076
                     /locus_tag="PK976_06885"
     CDS             <1..1076
                     /locus_tag="PK976_06885"
                     /inference="COORDINATES: protein motif:HMM:NF019583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 92 protein"
                     /protein_id="HRC67647.1"
                     /translation="DGLIGNEDCGQMSAWLILSAMGFYPVTPGSDIYVLGTPWFKEME
                     VQFENGKTFTITTDHPSPKSFYVQKVLLNGQSYDKSFIRHQDIKSGSHLHFKMGKHPN
                     ETWGNPPESRPKSIIQDHLLLPMPYINSPDKRIRRSIQISMGAPIAGSEIYYTLGGTM
                     PDKTSLVYSQPIQLTESAQVIAIALHPEMGFSYPAMAEFVKIDTNLKIRLLSNYHPNY
                     SAGGPDALIDGLRGPDNWRLGGWQGYQGTDFEAIVDMGSKKTIHHCALGCLQDAGSWI
                     WMPSKVEFFLSDDGKNFQKIGEVAPNIATDDYRKQVKDLGITINRSGRYIKVRAINLG
                     TIPSWHPGAGDKTFIFVDEIVVK"
BASE COUNT          411 a          279 c          320 g          368 t
ORIGIN      
        1 ccgatggttt gattggcaac gaagattgcg gccaaatgtc ggcatggctt atattgagtg
       61 ctatgggatt ttatcccgtc actcccggca gcgacattta tgtattggga acaccttggt
      121 tcaaggaaat ggaagtccag tttgaaaatg ggaaaacctt caccattaca accgatcacc
      181 cttctccaaa atctttctac gtgcagaaag tactcctgaa tggtcagtcc tacgataaat
      241 cctttatccg gcaccaagat ataaaatccg gaagccattt acatttcaaa atgggaaaac
      301 atcccaacga aacctggggt aatccgcccg aaagccgtcc caaatcaatc atacaggatc
      361 atcttctttt gcctatgcct tacattaatt cgcccgacaa aagaatccgc cgaagcattc
      421 aaataagtat gggagctccc atcgcaggga gtgaaattta ttataccctc ggtggaacaa
      481 tgcctgataa gacctcattg gtttacagcc aacctataca actgactgaa tcggctcagg
      541 taatagccat tgccttgcat cccgaaatgg gattcagtta tccagccatg gctgaattcg
      601 taaaaataga tacgaattta aaaatacggc tgttatcgaa ttatcatccc aattattctg
      661 caggcgggcc tgatgccttg attgatggct tacgcggacc cgataactgg cgtttaggcg
      721 gctggcaagg ttatcaagga acggatttcg aagccattgt ggacatgggc agcaaaaaaa
      781 ccatacatca ttgtgccctt ggatgtttac aagacgcagg ttcatggatc tggatgccct
      841 cgaaggtcga gttttttctt tctgatgatg ggaagaattt ccagaagatt ggcgaagttg
      901 ctccaaatat tgccaccgac gattatcgga aacaggtgaa ggatcttgga atcactataa
      961 atcgcagcgg acgctatatc aaggtaagag ccatcaattt aggaactatt ccttcatggc
     1021 accccggcgc aggcgacaaa accttcattt ttgtggatga aattgtcgtg aaatgatggg
     1081 aattagtgga tggtgaatgg agacgaaggg acaacgagac tgggagactg ccacaaggag
     1141 accaggagac gtggagacga ggagacaagg tggatggtga ttggtgatta gaatttgtaa
     1201 tttataattt gttttttata ggctgtcagt tgttagttat caagtttgca gtgctcaacg
     1261 aggaatagcg gacttcaatg tatctggtta ttaaaaaata aaccctattc aagcgtaaca
     1321 gattaaattt gcgaaaaata tcatcatgcg tgtacatact gattttttag tgattggt
//
LOCUS       DAORPD010000573         1377 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_19184_length_1377_cov_2.152799, whole genome shotgun sequence.
ACCESSION   DAORPD010000573 DAORPD010000000
VERSION     DAORPD010000573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19184_length_1377_cov_2.152799"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /locus_tag="PK976_06890"
     CDS             <1..809
                     /locus_tag="PK976_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67648.1"
                     /translation="VPISSPLTVWTYLWQNLYPEQIALNTSGWIGPLILALVLGPPLG
                     FFVLFGFLRLRKETIRIAAPALVCFLIGTFLQNRYHQLFIAFPFIVLTGSHGWTDFIE
                     KSKSWSSRYRSLYYAMLGYFWTANSILLIWISTVAPHQPLIDALEYIRKNPKVNVIVA
                     VEPTSDFFEPVPVYYAAKSIQVKYISNAKDIDSLRNHIRMHSRDSLPGYVLFLGEKNL
                     KQRIKTVSEIMPSLSVNAIFKAGFTERNIRRILHQPTSGSVIVYKNDYFY"
     gene            825..>1377
                     /locus_tag="PK976_06895"
     CDS             825..>1377
                     /locus_tag="PK976_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HRC67649.1"
                     /translation="MELEHARTRIEELTEMIEQHNYNYYVLNAPTISDYEFDKLMAEL
                     IKLEKQFPQLASPYSPTQRVGGEITKEFQTVRHRTPMLSLSNTYTDTEIRDFDQRVRK
                     TIGNEVEYVCELKFDGVAISLIYEDGKLIRGVTRGDGTQGDDVTANVKTIRSIPLRLK
                     GDFPSQLEMRGEIYMTHAEFNRLN"
BASE COUNT          417 a          264 c          269 g          427 t
ORIGIN      
        1 aggttcctat aagttctcct ctaacggttt ggacttattt atggcaaaat ctttaccccg
       61 aacaaattgc gttgaatact tccggatgga ttggaccact tattcttgcc cttgttttgg
      121 gtcctccttt gggttttttt gtattattcg gatttttacg cctcagaaag gaaactattc
      181 gtatcgctgc tcctgctttg gtttgttttc tgataggcac ctttcttcag aatcgatacc
      241 atcaactttt tattgccttc ccatttattg ttctaaccgg cagtcatggc tggactgatt
      301 ttatcgaaaa gagcaaatca tggtcgagcc gttaccgatc cttatactat gccatgctag
      361 gatatttctg gacagcaaac agtattttat tgatctggat ttcgacggtt gcacctcatc
      421 aacctcttat cgatgcctta gaatatatta gaaaaaatcc aaaagtgaat gttattgtcg
      481 ccgtagaacc aacatctgat ttcttcgaac ctgttccagt gtattatgcc gctaagtcta
      541 ttcaagtaaa atatatttcg aatgccaaag acattgattc gttgagaaat cacattcgaa
      601 tgcattcgag agattctttg cctggctatg ttttgttttt aggagaaaaa aatttgaaac
      661 aaagaattaa aaccgtttct gaaataatgc cttcattgag tgttaatgca attttcaagg
      721 caggatttac cgaacgaaat attcgccgaa ttttacatca acctacttcg ggaagcgtta
      781 tcgtttataa gaatgattat ttttattaaa aattcgaaat tatcatggaa ctcgaacacg
      841 cacgaactcg tatcgaagag cttacagaga tgatcgaaca gcataactac aattattatg
      901 tattgaatgc tccaaccatc agtgattatg agtttgacaa gctaatggcc gaattgatca
      961 aactcgagaa gcaattccct caattggcct ctccctattc gcccacacaa agagtgggag
     1021 gagagataac caaagaattt caaacagtaa ggcatcgcac acctatgctg tcgctttcca
     1081 atacatatac cgatactgaa attagggatt ttgaccaaag agttagaaaa acgataggca
     1141 atgaagtgga atatgtttgc gagttaaaat ttgatggagt ggcaatcagt ttgatatatg
     1201 aagatgggaa actgataagg ggagtaaccc gtggtgatgg tactcaggga gatgatgtaa
     1261 ctgcaaacgt aaaaaccatt cgcagcattc ctttgcgatt aaaaggggat tttccttcac
     1321 aactcgaaat gcgtggcgag atctatatga cacatgccga attcaaccgt ttgaatc
//
LOCUS       DAORPD010000574         1376 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_19208_length_1376_cov_2.173354, whole genome shotgun sequence.
ACCESSION   DAORPD010000574 DAORPD010000000
VERSION     DAORPD010000574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1376
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19208_length_1376_cov_2.173354"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="PK976_06900"
     CDS             <1..416
                     /locus_tag="PK976_06900"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HRC67650.1"
                     /translation="AWLYSSFGSNFVKTIRRNALQKQNLDVVSDQVGSPTWAADLAEM
                     ILHVIIHRIEPEGVEILHYTNEGVCSWYDFAISIVEHSDISCTIHPISSEQYPLPAPR
                     PFYSVLDKTNTRQRLNIEIPHWNASLKKCIQLLNE"
     gene            409..795
                     /locus_tag="PK976_06905"
     CDS             409..795
                     /locus_tag="PK976_06905"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HRC67651.1"
                     /translation="MNNTDRNRIIAQIESMMNQIWEKLGEGLPLATYRECLIFELRSA
                     MLSFRENVVIPVTYKYFKPENQLKVDLLIENEIPVEIEMESSEAHRKMMAVLRHSGKP
                     IGLLITLQVKPVRFILNKITYPQKIR"
     gene            798..>1376
                     /locus_tag="PK976_06910"
     CDS             798..>1376
                     /locus_tag="PK976_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HRC67652.1"
                     /translation="MNKINPAILEKAHHWTQPPFDEQTRQKVQYLLDNDPAELIESFY
                     TDLEFGTGGLRGIMGPGTNRMNIYTVGMATQGLANYLKMNFPDKNPIKVVIAHDCRNN
                     GRLFAQTTANVLSANGIKVFLFDDLRPTPELSFAVRFLGCQSGVVITASHNPKEYNGY
                     KAYWEDGGQLVSPHDKNVMAEVSKIKGIEEVKF"
BASE COUNT          448 a          294 c          279 g          355 t
ORIGIN      
        1 cagcttggtt gtattcgagt ttcggaagca attttgtaaa aaccattcgc cgcaatgctt
       61 tacaaaaaca aaacctcgat gtagtctccg accaggttgg ttcgcctact tgggcagccg
      121 atctggccga aatgatcctc catgttatta ttcatcggat agaacccgaa ggagttgaaa
      181 ttctgcatta taccaatgag ggggtgtgca gttggtacga ctttgccatc agcattgtag
      241 aacattcaga tatcagttgt acaattcatc cgatatcatc cgaacaatat cccttaccag
      301 ctccacgacc attttacagc gttctcgata aaacgaatac acgtcaacgc ctgaatatcg
      361 aaattccgca ttggaacgcc agtttgaaaa aatgtataca attgctgaat gaataatacc
      421 gatcgaaatc gtatcattgc tcaaatcgag agcatgatga atcaaatatg ggaaaaattg
      481 ggagaaggcc ttcctcttgc cacttacagg gaatgcttga tttttgaatt gcgctcagca
      541 atgttatcat tccgtgaaaa tgtggtcatc ccagtaacct acaaatattt caagccagaa
      601 aaccaactaa aggtcgatct tttgatagaa aatgaaatac ctgtagaaat tgaaatggaa
      661 tcaagcgaag cacacagaaa gatgatggcc gtactccgcc actcgggcaa acctattgga
      721 ttgttaataa ctttgcaggt taaacctgta cgattcattc ttaataaaat tacttatcct
      781 caaaaaatac ggtaaccatg aacaagataa atccagctat tctcgaaaaa gcccatcact
      841 ggacacaacc gccttttgac gaacaaacgc gccaaaaggt tcaatatctt ttagataacg
      901 atccagcaga actcatcgaa agtttttata ccgacctgga atttggtaca ggtggactgc
      961 gaggtattat gggacccggg actaaccgaa tgaatatcta tacggtagga atggccacac
     1021 aaggcttggc aaattattta aaaatgaact tccccgataa gaatcccata aaagtagtca
     1081 tagctcatga ttgccgcaac aacggacgcc tttttgcaca aactacggca aacgtattat
     1141 cggccaatgg aatcaaagtt ttccttttcg acgatttaag gcccacaccc gagctttcgt
     1201 ttgctgtgag atttcttgga tgccagagtg gcgtagttat tactgcttca cacaatccta
     1261 aagaatataa tggatacaag gcttattggg aagacggcgg ccagcttgtg agcccccacg
     1321 acaagaatgt catggcagaa gttagcaaga taaagggaat agaagaagtg aaattc
//
LOCUS       DAORPD010000575         1371 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_19333_length_1371_cov_2.126900, whole genome shotgun sequence.
ACCESSION   DAORPD010000575 DAORPD010000000
VERSION     DAORPD010000575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1371
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19333_length_1371_cov_2.126900"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="PK976_06915"
     CDS             complement(<1..889)
                     /locus_tag="PK976_06915"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HRC67653.1"
                     /translation="MIKTLRTQSLLSYLALSLLLMSCSRKPEKTIITGEAQGTYYQVT
                     YLDVAKRNFSKEIDSLLHAFDRSVSLWDDSSIICRINRNETGVKTDSVFRFVFTQSME
                     VAKRTDGAFNPCVGPLVKAWGFHRKQGKMPDSLLIIELLKHIDYRNIWLQGDSVVKSD
                     TSIELDFNAIAQGYSADLLASFLRQKGITSYLVDIGGEVVTGDPKPDGNQWRVGIEKP
                     SDSASSEREIEAVVEIAHKAIATSGSYRKYRIENGRRYSHTIDPKTGYPVNHNLLSVS
                     VVAKDGITADAYATAFMVMG"
     gene            967..1140
                     /locus_tag="PK976_06920"
     CDS             967..1140
                     /locus_tag="PK976_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HRC67654.1"
                     /translation="MDSGIYKCGLCEYMYRPQKGNPENGIAPGTPFDELPDDCICPRC
                     GNPKTYFYSTDDE"
     gene            complement(1119..>1371)
                     /locus_tag="PK976_06925"
     CDS             complement(1119..>1371)
                     /locus_tag="PK976_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67655.1"
                     /translation="TEAYSASIQAAQKCDVLIVIGTTGEVMPANLIPGLAKNNGARII
                     EINPHISEHTQVVTDVFLPIKASEAMMALDEAIYSSSVE"
BASE COUNT          376 a          318 c          272 g          405 t
ORIGIN      
        1 ctcccattac cataaatgct gttgcataag catcggctgt aatgccatct tttgccacaa
       61 cacttacact taacaaatta tgattgacag gataacctgt tttggggtca atcgtatgag
      121 aatatcgcct tccgttctct atacgatatt tcctgtaact tcccgaagta gcaatggctt
      181 tgtgggctat ttccaccacg gcttcaattt ccctttctga gctcgcactg tcggatggtt
      241 tttctattcc aactctccat tgatttccat cgggtttcgg atctcccgta actacttccc
      301 cgccaatatc aaccaaatag gaagtgattc ccttttgccg taaaaatgaa gccagcaaat
      361 cggcagaata accttgtgct atggcgttga aatcgagttc tatgcttgta tccgatttca
      421 caacactatc gccttgcaac cagatgttcc gataatcgat atgtttcaac aattcaataa
      481 tcaagaggct atcgggcatt tttccctgct tgcgatgaaa tccccaagcc ttcaccaacg
      541 gccctacgca cggattgaaa gctccatcag ttctcttggc tacttccatc gattgggtaa
      601 acacaaagcg gaaaacacta tcggttttta cacctgtttc gttacggttg atacggcaaa
      661 tgatgctact atcgtcccat aacgagaccg aacggtcgaa ggcatgcaaa agggaatcta
      721 tttccttgct aaagttcctc ttcgcaacat ctaaataagt tacctgataa tatgttcctt
      781 gagcctcacc ggtgatgatt gtcttctcag gtttgcgact gcatgacatc aataatagcg
      841 ataaggcgag atatgacaaa agagactgag ttcgaagcgt tttaatcata atttctcgaa
      901 tgattaacat ttatccgcgt gcaaaattcg gtatttttgc tcgagattta aattttgaag
      961 cgtattatgg attctggtat ttataagtgt ggattgtgcg aatatatgta caggccacag
     1021 aaaggcaatc ccgagaatgg cattgcaccg ggtaccccct tcgacgaact tcccgacgac
     1081 tgcatttgcc ctcgttgtgg caatccgaaa acatattttt attcaacaga tgacgaataa
     1141 atggcttcat caagcgccat catggcttca gaggccttaa tgggaaggaa aacatcggta
     1201 acgacctgtg tgtgttcaga aatgtgtgga ttgatttcga taatccttgc tccattattc
     1261 ttcgctaacc caggaataag attagcgggc ataacttcgc ccgtagtgcc aataacaatc
     1321 aatacatcgc atttttgcgc tgcctgtata gaagcagaat aggcttcagt g
//
LOCUS       DAORPD010000576         1371 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_19341_length_1371_cov_1.904255, whole genome shotgun sequence.
ACCESSION   DAORPD010000576 DAORPD010000000
VERSION     DAORPD010000576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1371
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19341_length_1371_cov_1.904255"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..>1371
                     /locus_tag="PK976_06930"
     CDS             78..>1371
                     /locus_tag="PK976_06930"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRC67656.1"
                     /translation="MAFVFRSGVTIGGSYKEGKDVGNADIFVDVYPPDLSINITSPQG
                     NHLIEAEGDTILVHAISPQADSMWVFENGSLRKAVAGVFMDDTLVASPINGYWNDHHL
                     IFVAKNSQQIVRDTLFYVVIPQPTVVELPDGIYDGINYVDDSTVILCLQAPGKKNVFA
                     IGDFSNWEILPEYYMNKTADGERFWLEISSLIPAKEYIYQYLVDGKIRISDPYCDKIS
                     DPWNDKYIPTSVYPAMLIYPSGKTTGIASVLQTAQEPFAWQIEQFSPPSREKLVIYEL
                     LIRDFTTKHTYQSVIDTLNYLVKLGVNAIELMPVMEFEGNSSWGYNPMTYFAPDKYYG
                     PKNDLKKLIDECHKRGIAVILDIVLNHAYGNNPLVKLYWDDVKQIPAADNPWFNQTSP
                     NPSYSWGYDFNHESPYTKQFVYRVTRYWLTEYKADGFRF"
BASE COUNT          428 a          252 c          289 g          402 t
ORIGIN      
        1 ccaattatta gagacttaca atttctcctg acatcaggtc ttattatggt gttccggcaa
       61 atgaacaaat tctgaaaatg gcttttgtct tcagaagtgg cgtcacaata gggggttctt
      121 ataaggaagg aaaagatgta ggaaatgccg atatttttgt ggatgtctat ccgcctgact
      181 tatccattaa cattacttct ccacagggga atcatttaat cgaagcggaa ggagatacca
      241 ttttggttca tgccatcagc cctcaggctg attcaatgtg ggtatttgaa aatggcagtc
      301 ttcgaaaagc cgtagcagga gtttttatgg atgataccct tgtggcgagt ccaataaatg
      361 gatattggaa cgatcatcat cttatttttg tagctaaaaa ttctcagcag atagtaagag
      421 ataccttgtt ttatgttgta attccgcaac ccacagtggt ggagttaccc gatggaatat
      481 acgacggcat aaattacgtt gacgattcaa cggttatatt atgtttgcag gctccgggga
      541 agaaaaatgt attcgcaatt ggcgatttca gcaattggga gattttgcca gaatattata
      601 tgaataaaac tgcagatgga gaaagattct ggttagagat aagcagtttg attcccgcca
      661 aagaatatat ctatcaatat ttagtggatg gaaaaatcag gatatccgat ccttattgtg
      721 ataaaatcag tgatccatgg aacgataaat atatccctac gtcggtttat cccgcaatgc
      781 taatttaccc aagtggaaaa actacaggca tagcgtcggt tcttcaaact gctcaagaac
      841 catttgcatg gcaaatcgaa caattttcgc ctccatcgag ggagaaactc gttatttatg
      901 aattgcttat ccgcgatttt acaacaaaac atacttatca atcggtcatc gacactttaa
      961 attaccttgt caaactggga gtcaatgcca tcgagctgat gccagtgatg gaattcgagg
     1021 ggaattcgag ctggggttat aatcccatga cttattttgc ccctgacaaa tactatggcc
     1081 ccaagaacga tttaaaaaaa ttgatagatg aatgtcataa aagaggaatt gctgttattt
     1141 tggacatagt gctgaatcat gcatacggga ataatcctct agtaaaacta tactgggatg
     1201 atgtaaaaca gattccggct gctgataatc catggttcaa tcagacttcg cccaatccaa
     1261 gctatagctg gggttacgat ttcaaccacg aaagtcctta tacaaaacaa tttgtatatc
     1321 gagtaacacg ctactggctt acagaatata aggcagatgg ttttcgtttc g
//
LOCUS       DAORPD010000577         1367 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_19411_length_1367_cov_2.808689, whole genome shotgun sequence.
ACCESSION   DAORPD010000577 DAORPD010000000
VERSION     DAORPD010000577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19411_length_1367_cov_2.808689"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..896
                     /locus_tag="PK976_06935"
     CDS             <1..896
                     /locus_tag="PK976_06935"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HRC67657.1"
                     /translation="IGTLKWHHILNPSEQDFDYLLEHFHFHPLDIEDCRSLVNQRPKI
                     DIYDDYYFLILHFPYFDKTRNFVRTREEKIFWGKDYVITMGKIHSEVSEFFLKTRQKI
                     EQHIDLDFATSDALLYRILEYLMKESYTLVRYVGDQVDQAGRMLFERKTLQTIESISV
                     TRRNIIILNTIFKPQLPVFNKFESGEIQSFAESMEEYWGNILDYYQKIWDLIEDYSEL
                     IEGYSKTFDSLQTNRTNEIVKTLTLVSTIVLPLTFITSVYGMNILLPLQENKYAFHII
                     TAVMVALAGFMLFYFKKRHWM"
     gene            complement(926..>1367)
                     /locus_tag="PK976_06940"
     CDS             complement(926..>1367)
                     /locus_tag="PK976_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67658.1"
                     /translation="RANALPAEFREPFGKIKYKTKQTRLNNYLNIFSSSRHIRSNKSK
                     RLWAKLFPTHFKHIRVVFINVSKHLDKIDLEIRQDLFERKRINEDGNTGKHHHKNKKD
                     HIDLVIHLDIILLNDELAQKILDFLAFRCELNDFPIPFEEAIYY"
BASE COUNT          379 a          252 c          295 g          441 t
ORIGIN      
        1 ggattggaac gctcaaatgg catcatatcc ttaatccctc ggaacaggat ttcgactatc
       61 ttcttgagca tttccatttt catccactgg atattgaaga ctgcagaagt ttggtgaatc
      121 agcggcccaa aatcgatatc tacgacgact attactttct tattttacat tttccctatt
      181 tcgacaagac acggaatttt gtacgtactc gcgaagaaaa gatattctgg gggaaggatt
      241 atgtgatcac aatggggaag attcattccg aagtatcgga gtttttcctg aaaacacgtc
      301 agaaaatcga acagcatatc gatcttgatt tcgcaaccag tgatgctttg ctttatcgca
      361 ttttagaata tttaatgaaa gaaagttata ccctggttcg atacgtgggt gatcaggtag
      421 atcaggccgg gcggatgctt ttcgaacgaa agactctgca gactattgaa tctatttcag
      481 taacgcgtcg taatattatc atcctgaata cgatttttaa gccccaactc ccggttttca
      541 acaaattcga atcgggtgaa atacaaagct ttgccgaaag catggaagaa tattggggca
      601 atatactcga ctattatcag aaaatctggg acttaatcga agattattcg gaattgattg
      661 aaggctattc caaaactttc gattcgttac aaaccaatcg taccaatgag attgtaaaaa
      721 cgcttaccct tgtttcgacc attgttctgc ctttaacctt cattaccagt gtttatggca
      781 tgaacattct tttgccccta caggaaaaca aatatgcttt tcatatcatt actgctgtaa
      841 tggtggcttt agcaggtttt atgttgtttt atttcaagaa acggcattgg atgtagacag
      901 ttcggggaaa tggttctata gagaattaat aataaatggc ttcctcgaag gggatgggaa
      961 aatcgttcag ttcgcatctg aaggcgagga aatcgaggat tttctgggcg agttcgtcgt
     1021 ttaacagtat aatgtcgaga tggatgacaa ggtcgatatg atcctttttg ttcttatgat
     1081 gatgttttcc cgtatttcca tcttcgttga tgcgttttcg ctcgaatagg tcctgcctga
     1141 tttcgaggtc gattttgtcg agatgtttcg aaacgtttat aaaaaccacc ctgatgtgct
     1201 tgaagtgtgt ggggaataat ttagcccata gtcttttgct tttattgcta cggatgtgcc
     1261 gactgctcga aaagatgttc aaataattat tgaggcgagt ttgttttgtc ttgtatttga
     1321 tttttccaaa aggctcacga aattcggcag gcagcgcatt agccctg
//
LOCUS       DAORPD010000578         1367 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_19420_length_1367_cov_1.839939, whole genome shotgun sequence.
ACCESSION   DAORPD010000578 DAORPD010000000
VERSION     DAORPD010000578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19420_length_1367_cov_1.839939"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..>1367
                     /locus_tag="PK976_06945"
     CDS             52..>1367
                     /locus_tag="PK976_06945"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HRC67659.1"
                     /translation="MYNTLKLYRFNILIPLRANILRNWDYIRKWRLRKISDRQLVNIL
                     GVIVGFLSGLSAIILKNTVYYTHAFITSDFGFGNNSFLLFFSPLIGILLTVLFIRYFI
                     KDDISHGISKVLYAISKNNSLLKPHNTFSSMIASTLTIAFGGSVGAEAPIVLTGASIG
                     SNLGRIAKVSYKHRTLLIGCGAAGAIAGIFKAPIAGIVFTIEVIMIDLTMATLIPLLL
                     SAITASVISFFFLGDDVLFSFTLESSFDLSRLPFYAILGLFCGFVSLYFTRASDYTES
                     KLQRIGNPFHRLLVGGVILGIFIYIFPSLYGEGYDALMLMLNGHGTEVVNQSLFGNLQ
                     MSSFMFPLILLVIMLTKVIAMALTNAAGGIGGVFAPSLFMGGMSGLFFTRTLDLLTGI
                     RMPENNFVLVGMAGVMAGVMHAPLTAIFLIAEITGGYQLLTPLMLTA"
BASE COUNT          325 a          248 c          300 g          494 t
ORIGIN      
        1 gaaatgcaat cagattctcg ttaataaaat gggattattc ctaccaaaat tatgtataat
       61 acattaaaat tatacaggtt caatatcctt attccattaa gggctaatat tttaaggaat
      121 tgggattaca tcaggaaatg gcggctgaga aaaatttctg accgtcagtt ggtcaatatt
      181 cttggagtta ttgtaggctt tttgagtggg ttgagtgcta ttattctgaa aaatacggtt
      241 tattatacgc atgcttttat tacgagtgat tttggctttg gtaacaacag cttcctattg
      301 ttcttttcgc ccttaatagg gatattgctt acagtgcttt ttattcgtta ttttataaaa
      361 gatgatatat cacatggaat aagtaaagtg ttatatgcta tttcgaagaa taacagtttg
      421 ttgaagcctc ataatacttt ttcttcgatg atagccagca ctttaacgat tgcctttgga
      481 ggttcggtag gggctgaggc tcccatagtg ctcacaggtg cttctatagg aagcaatttg
      541 gggcggattg caaaggtttc atacaaacat cgaaccttac tgattggatg cggagcggct
      601 ggagctatag ccggtatttt taaggccccc atcgctggta ttgtttttac catcgaggtt
      661 atcatgatcg acctgactat ggctacctta atacctcttc ttttatcggc tatcacggcc
      721 tctgtcattt cttttttctt tcttggggat gatgttttgt tttcatttac cctcgaaagt
      781 tcgttcgatc tctcacgtct tccattttat gctatattgg gcttattttg tggctttgtt
      841 tccttatatt ttacgagggc ttccgattat acggaatcca agctgcagcg catcggtaat
      901 ccttttcatc gacttttagt gggaggtgtt atactgggaa tttttatcta tatatttcct
      961 tcactttatg gggaaggata tgatgctttg atgctgatgc tcaatggcca tggcaccgaa
     1021 gtggtcaacc agagtttgtt tggaaatctg cagatgtcgt ccttcatgtt tccattgatc
     1081 ttgcttgtaa tcatgttgac caaagtaata gccatggctc tgacaaatgc tgcaggtggt
     1141 attggtgggg tttttgcgcc ttctctgttt atgggtggca tgagtggttt gttttttact
     1201 cgcacgctcg atcttcttac gggaattcga atgcccgaaa acaacttcgt tctggtagga
     1261 atggctggtg taatggcagg agttatgcac gctcctctta cggctatatt cctgattgcc
     1321 gaaattacag gaggttatca attgcttacc cctttaatgc tgactgc
//
LOCUS       DAORPD010000579         1365 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_19466_length_1365_cov_1.668702, whole genome shotgun sequence.
ACCESSION   DAORPD010000579 DAORPD010000000
VERSION     DAORPD010000579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19466_length_1365_cov_1.668702"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="PK976_06950"
     CDS             complement(<1..712)
                     /locus_tag="PK976_06950"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HRC67660.1"
                     /translation="MKKQKSIVILFFTFVLSMMLDACTKEENGGCITSTGKIVYEERS
                     LLASPDTLIIEGEMDVYITMDRKPRLYFQSGENLLAGVVSTLIGKKLTLEDFNTCDFL
                     RNMGVRTKVFLTVTPQSFLFIEFESSGVISSTNTLSLDSIRIECRNGGGTVNLDIETA
                     KAWFIQHSGTGSVDFHIHGKSGYTFVYCMGYGPFDLRDLCTTGYLHFVHLGSNNCWVN
                     AGGILQAEIWSKGDLYYTG"
     gene            complement(717..1133)
                     /locus_tag="PK976_06955"
     CDS             complement(717..1133)
                     /locus_tag="PK976_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67661.1"
                     /translation="MSGALKEIFPVGGKQYYALNLSLNGLTPVSRYFISGLGLDITWD
                     QSDKELLARQGIYVDNEAEIINYGVNLFGGLRIGKLDLCLHAGTYLYRKEKSDNIIYD
                     KIVASYTIFEKMQLYLTLKTHFAKADYFAAGLGYQF"
     gene            1132..>1365
                     /locus_tag="PK976_06960"
     CDS             1132..>1365
                     /locus_tag="PK976_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67662.1"
                     /translation="MATAKQAPMQSPEQLFADIHPAQILIVEDNAELAHYLRSVIAPH
                     YHTELAINGQDGIEKALESIPDLIISDVMMPVKD"
BASE COUNT          400 a          317 c          259 g          389 t
ORIGIN      
        1 agcccgtata atataagtcg cctttgctcc agatttccgc ttgcagtatg ccaccagcat
       61 tcacccagca attgttcgaa cctaaatgta caaaatgcaa ataacctgtg gtgcacaaat
      121 cacgtaaatc gaaggggcca taacccatac aataaacgaa ggtatatccc gatttcccat
      181 ggatatgaaa atcaaccgag cccgtgccgc tgtgctgaat aaaccatgct ttcgcggttt
      241 ctatgtctaa atttacggtt cctcctccgt ttctgcattc tattcgtatt gaatccaacg
      301 atagagtgtt ggtcgaagat atcacacctg aactttcaaa ttcgataaat aggaatgatt
      361 gaggcgttac tgtaaggaat accttggttc taacgcccat attgcgcaaa aaatcacaag
      421 tattgaagtc ttcgagggtt agttttttcc cgattagagt ggatactaca ccagctaaca
      481 gattttctcc cgattgaaaa taaagccgtg gttttctgtc catagtaata tatacgtcca
      541 tttctccttc gatgatcaat gtatcgggag atgcaagcaa ggaacgttct tcataaacaa
      601 tctttcctgt cgaagtgata cagccgccat tctcttcctt tgtacaggca tccaacatca
      661 tactgagtac gaaagtaaaa aataagataa cgatggattt ctgttttttc attggtttaa
      721 aattgatagc ccaggccggc tgcaaaataa tcggctttgg caaagtgggt tttcaaggtg
      781 agatacagtt gcattttttc aaagatagtg tatgaggcta ctattttgtc gtagatgatg
      841 ttatccgatt tttcctttct gtagagataa gttcccgcat gcaaacaaag atcgagttta
      901 ccaattcgca aaccgccgaa caaatttact ccataattga tgatttcggc ttcattgtct
      961 acgtatattc cctgtcgtgc caataattcc ttatcagatt ggtcccaagt aatatccagt
     1021 ccaagcccgc tgataaaata tctcgaaaca ggtgtcaatc cgttcaacga caaattcaat
     1081 gcataatatt gttttccacc taccgggaaa atctctttca acgctcctga catggctacc
     1141 gccaaacagg cgcctatgca aagccccgaa caactttttg cggatattca cccggcgcaa
     1201 atcctgatcg tggaagacaa tgccgagctg gcccattacc ttcgatctgt aattgcacct
     1261 cattaccata cagaattggc cattaacgga caggatggca tagaaaaagc ccttgaatcc
     1321 atccccgacc tgatcataag cgacgtgatg atgcccgtta aggac
//
LOCUS       DAORPD010000580         1364 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_19485_length_1364_cov_2.585180, whole genome shotgun sequence.
ACCESSION   DAORPD010000580 DAORPD010000000
VERSION     DAORPD010000580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19485_length_1364_cov_2.585180"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..839
                     /gene="panB"
                     /locus_tag="PK976_06965"
     CDS             15..839
                     /gene="panB"
                     /locus_tag="PK976_06965"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HRC67663.1"
                     /translation="MNESFGSQRYTKITTHVLQEMKLKGEKIAMLTAYDYSMAGILDA
                     AGIDVILVGDSASNVMAGYTTTLPITLDEMIYHASSVVRAVQRALVVVDLPFGTYQGN
                     SKMALASAIRIMKESGANAIKIEGGKEIIESVERILSAGIPVMGHLGLTPQSIHKFGT
                     YVVRAKEETEANKLKEDAQLLEKAGCFAIVLEKIPAALGAEVTQLVKCPIIGIGAGSG
                     VDGQVLVLHDMLGITIKFSPRFLRRYHNLYEEIKGAIQTYIEDVKNLDFPNAREQY"
     gene            860..>1364
                     /locus_tag="PK976_06970"
     CDS             860..>1364
                     /locus_tag="PK976_06970"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HRC67664.1"
                     /translation="MAKFIDLSQRILYEDNHLLVINKLPGEIVQGDKTGDKPLVEIAK
                     DYIAQKYDKPGNVFLGVVHRLDRPVSGAIILARTSKALERLNAMLQERKIMKTYWAIV
                     KNKPPKENDTLVNYVWRNKQKNKTIVFDHPIKKSSEASLTYRIVAQSVNYYLLEIGLH
                     TGRHHQIR"
BASE COUNT          420 a          265 c          301 g          378 t
ORIGIN      
        1 caaagaatct gattatgaat gaaagttttg gttcacagcg atatacgaag atcaccactc
       61 acgtacttca ggagatgaaa ctaaagggcg agaagatagc catgcttacg gcctatgatt
      121 attcgatggc tggaattctc gatgctgccg ggatagatgt gattctggtt ggcgatagtg
      181 cttccaatgt aatggcaggt tatacaacta ctcttcctat tacgcttgac gaaatgattt
      241 atcatgccag ctcggtagtg cgtgccgtac aaagagcatt agtcgttgta gatttgcctt
      301 ttggtactta tcagggaaat tctaagatgg ctttggcttc ggccattcgc atcatgaagg
      361 aaagtggtgc caatgccatt aagattgagg gaggcaaaga aatcatagaa agtgtggaaa
      421 gaatactttc ggctggcatt cccgtgatgg gccatttagg attgacgcct caatctattc
      481 ataagtttgg tacttatgta gttagggcca aagaagaaac cgaagccaac aaactgaaag
      541 aagatgccca attgctcgaa aaagcgggtt gctttgctat cgtgctcgaa aaaattcctg
      601 ctgctttggg tgccgaagtt actcaattgg taaaatgccc gattattggc attggtgctg
      661 gttctggcgt ggatggacag gtattggttt tgcatgatat gttgggtatt actatcaaat
      721 tttctcctcg ttttctgcgt cgctaccaca acttgtatga agaaataaaa ggagccattc
      781 aaacctatat agaagacgtg aaaaacctcg actttcccaa tgcccgtgaa caatactgag
      841 tttgcaagga ttttcatcta tggcgaaatt tatcgatctt tcgcaacgta tcttatatga
      901 agataaccac ctgcttgtta tcaacaaact tcctggcgaa atcgtacagg gagataaaac
      961 aggagataaa cccctcgttg aaattgccaa agattatata gcccagaaat acgataagcc
     1021 aggaaatgta tttcttgggg ttgttcatag gctcgaccgt cccgtaagtg gagctatcat
     1081 tttggcccgc acaagcaaag ccctcgaacg gctcaatgct atgcttcagg aaaggaaaat
     1141 tatgaaaacg tattgggcta ttgttaaaaa taaacctcca aaagaaaatg atacactcgt
     1201 caactatgtt tggagaaata aacagaaaaa caaaaccatc gtattcgatc atcctattaa
     1261 aaaaagttct gaagcatctc taacttatcg tattgtggca caaagtgtca attattatct
     1321 tctcgaaata gggctccata ctggccgcca tcatcaaatc agag
//
LOCUS       DAORPD010000581         1363 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_19506_length_1363_cov_2.051988, whole genome shotgun sequence.
ACCESSION   DAORPD010000581 DAORPD010000000
VERSION     DAORPD010000581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19506_length_1363_cov_2.051988"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1038
                     /locus_tag="PK976_06975"
     CDS             <1..1038
                     /locus_tag="PK976_06975"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HRC67665.1"
                     /translation="HQKGIEYVLGILIHPEDGSLKGIHEVDAVGHRVVHAGEKFGGSV
                     RINHDVVGALEECIDLAPLHNPPNLEGIYAITRLLPQIPQVGVFDTAFHQTMPDYVYL
                     YGLPYSLYEKYKIRRYGFHGSSHKYVSLRAAKILGQDYDKLRLITCHLGNGASVAAIK
                     NGKSFDTSMGMTPIEGLIMGTRTGDVDAGALLHIAEKEGIGFQTLNKLINKFSGLLGI
                     SGISSDMRDLEEAAAKGHERAIIALNMFRYRVKKYVGAYTAAMGGVDAIVFTGGIGEN
                     DAASREEIMKDMEWLGIDFDFEKNRSVKSQENIITREDSKVKVMVIPTNEELVIASDT
                     YEIVTRNLEMI"
     gene            1101..>1363
                     /locus_tag="PK976_06980"
     CDS             1101..>1363
                     /locus_tag="PK976_06980"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HRC67666.1"
                     /translation="MRRLTVWLIVCGGLFHLNCPAQNLPSDKHFHHNLLTIDSHPDTP
                     LMLLRPGTDFMQDNSANPYSRVDYPRMQKGGLDAVFFAVFIPQG"
BASE COUNT          388 a          278 c          326 g          371 t
ORIGIN      
        1 caccagaaag gaatcgaata tgttcttgga atcttgattc accccgagga tggtagcttg
       61 aaaggaatac acgaagtgga tgctgtcggg catagggtgg ttcatgcggg tgaaaagttt
      121 ggtggaagtg tacgtataaa tcacgacgta gtcggtgcat tggaagaatg cattgatctg
      181 gcacccttgc acaatcctcc caatctcgaa ggaatttatg ccattacccg acttcttccg
      241 caaattccac aggttggcgt tttcgacacg gctttccatc aaaccatgcc cgattatgtc
      301 tatctttatg gcttacccta ttcgctctac gaaaagtata aaatcagacg atacggattt
      361 catggtagca gtcataaata tgtgtcctta cgtgctgcga agattttggg acaggattat
      421 gataaattgc ggctgattac ctgtcattta ggaaatggag ccagtgtagc ggctatcaag
      481 aacgggaaat cattcgatac ttctatggga atgacaccta tcgaaggcct gatcatgggt
      541 acacgtacgg gtgatgtcga tgcaggggca ttgcttcata tagccgaaaa ggaaggaata
      601 gggtttcaaa ccctcaacaa actcattaat aaatttagtg gtttgctggg aatttcaggc
      661 atttcgagcg atatgcgcga tttggaagaa gctgccgcca aaggccatga aagagccata
      721 atcgccctga atatgttccg ctatcgcgtg aaaaaatatg taggagcata taccgctgct
      781 atgggtggcg tcgacgctat cgtctttacg ggtggaatcg gagaaaatga tgctgcttcg
      841 cgcgaagaaa ttatgaaaga catggagtgg ctgggaatag atttcgactt cgagaaaaat
      901 cgtagcgtga agagccaaga aaacataatt acccgtgagg attcgaaggt aaaagtcatg
      961 gtaattccaa ccaacgagga actcgtcatt gcctccgata cttatgaaat tgttacccga
     1021 aatctcgaaa tgatctgacc ttcttcgagg attaaggcat catttccttc gatgatcatc
     1081 taactcggaa atatattcac atgcgacgac ttactgtatg gctgatagtt tgcgggggac
     1141 ttttccattt gaattgtccg gctcagaact taccttctga caaacatttc catcataact
     1201 tattaaccat tgattcccac ccagatactc ccttaatgtt attaaggccg ggaactgatt
     1261 tcatgcagga taatagcgct aatccttata gcagagtgga ttatcctagg atgcagaaag
     1321 gcggactcga tgccgtgttt tttgcggttt ttatacccca ggg
//
LOCUS       DAORPD010000582         1360 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_19565_length_1360_cov_1.946360, whole genome shotgun sequence.
ACCESSION   DAORPD010000582 DAORPD010000000
VERSION     DAORPD010000582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19565_length_1360_cov_1.946360"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /gene="infC"
                     /locus_tag="PK976_06985"
     CDS             <1..526
                     /gene="infC"
                     /locus_tag="PK976_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HRC67667.1"
                     /translation="PAAKNQEEPYRVNEKIKAPIVRVVGDNFEQRIVTIKEALEIADQ
                     MELDLVEISPNANPPVCKLIDYSKFMYELKRKQKEAKAKAAKVILKEIRLGPNTDEHD
                     FNFKLNHARKFLEEGAKVKVEVLFKGRTIVYKEKGEIILLEFAQALEDIGKLESLPKL
                     EGKRMFITIAPKKS"
     gene            576..770
                     /gene="rpmI"
                     /locus_tag="PK976_06990"
     CDS             576..770
                     /gene="rpmI"
                     /locus_tag="PK976_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HRC67668.1"
                     /translation="MPKMKSKSSAKKRFTYTGTGKIKRKHAFKSHILTKKDTKRKRNL
                     THSTLVSKADYSTVKKMLAC"
     gene            853..1194
                     /gene="rplT"
                     /locus_tag="PK976_06995"
     CDS             853..1194
                     /gene="rplT"
                     /locus_tag="PK976_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HRC67669.1"
                     /translation="MPRSVNVVTSHRKRRKFLRRTRGQFGRRKNVWTVARNSWEKGQL
                     YAYRDRRAKKRSFRALWITRINAAVRQYGMSYSEFMGKLDKANIRLNRKVLADLAMNH
                     PDAFKALVDQL"
     gene            1267..>1360
                     /locus_tag="PK976_07000"
     CDS             1267..>1360
                     /locus_tag="PK976_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005678424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HRC67670.1"
                     /translation="MTTKERYKRFIAWFSENMPEASTEPVYQNPY"
BASE COUNT          500 a          285 c          255 g          320 t
ORIGIN      
        1 cccggccgca aaaaatcagg aagaacccta tagggtaaac gaaaagataa aagcgcccat
       61 tgttcgggta gtaggtgata atttcgaaca aagaatcgtt accataaagg aagccctcga
      121 aatagccgat caaatggagc tggatctggt agaaatttca cccaatgcca acccacctgt
      181 ttgcaaactc atcgattaca gtaaatttat gtatgaactc aaacgcaagc aaaaagaggc
      241 aaaggcaaaa gcggccaagg taatattaaa ggaaatacgt ttaggaccta ataccgacga
      301 gcatgatttc aactttaaac tcaatcatgc ccgtaaattc ctcgaagaag gcgcaaaagt
      361 aaaggtagaa gttcttttta agggtagaac catcgtttat aaggaaaaag gagaaattat
      421 ccttctcgaa tttgcccaag cactcgaaga tattggcaaa ctcgagagcc ttccaaaact
      481 cgaaggaaaa cgaatgttta tcaccatagc acctaaaaaa tcttaaagaa taagaaataa
      541 ataaaaaaac cgttcataat aataaaagtc aaaagatgcc taaaatgaaa tcgaaatcca
      601 gtgctaagaa acgttttact tacactggaa caggaaaaat caagcgcaaa catgctttta
      661 agagtcatat cctgactaaa aaggacacca agcgaaaacg taatcttact cattccactc
      721 tggtatccaa agccgattac tcaacagtaa aaaagatgct cgcttgctaa taatttactt
      781 tatgtctaac cttacgttca gctcatcaag attctgaaac cgtcagaacg ctgacgtaaa
      841 aaaaacaaaa aaatgccaag atcagttaat gtagttacat cacacagaaa aagaaggaag
      901 ttcctcagaa gaactcgtgg tcaatttggc cgtcgtaaaa atgtgtggac cgttgcaagg
      961 aactcatggg agaaaggcca attgtatgct taccgcgacc gacgtgccaa gaaacgctct
     1021 ttcagggcat tatggattac tcgtatcaat gcagccgtac gccagtatgg aatgtcttat
     1081 tccgaattta tgggtaaact cgataaagcc aatattcgcc tcaacagaaa agtattggcc
     1141 gaccttgcca tgaatcatcc cgacgccttt aaagcattgg tcgatcaatt ataggcagca
     1201 attaccttaa acctgaaagc ggcccttgat gtattacaag cgccgctttc tttttactta
     1261 ttcagtatga caaccaaaga acgctacaaa agattcattg catggttttc ggaaaatatg
     1321 ccagaagctt cgactgaacc ggtatatcaa aatccctacc
//
LOCUS       DAORPD010000583         1358 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_19593_length_1358_cov_2.783576, whole genome shotgun sequence.
ACCESSION   DAORPD010000583 DAORPD010000000
VERSION     DAORPD010000583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19593_length_1358_cov_2.783576"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..141)
                     /locus_tag="PK976_07005"
     CDS             complement(<1..141)
                     /locus_tag="PK976_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018694643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRC67671.1"
                     /translation="MLCRLSVKNYALIEELEFEPGPGFNIITGETGAGKSILLGALGL
                     ILG"
     gene            complement(203..1099)
                     /locus_tag="PK976_07010"
     CDS             complement(203..1099)
                     /locus_tag="PK976_07010"
                     /inference="COORDINATES: protein motif:HMM:NF027445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HRC67672.1"
                     /translation="MRRFGIFIGILLLSIASIGQELDCQISVSTRQVEGTDRQVYNTL
                     QTALFEFMNNKRWTNYQFKLEERIECTMLIQISERLSVDEFKGTMNVVLRRPVYKSSY
                     NSILLNHVDKDIQFNYVEFEPLDFQESAITSNLTAILAYYAYILIGLDFDSFSLYGGT
                     PFYEKAQTIVNMAQNLPQKGWKSFESMRNRYWLAENFNNTSYNKIRQFIYQYHRQGLD
                     LMAENVDQGRTNIIESLELMRQAYRDKPNSFLLQLILDAKRDEFINILTGAPDQDKVK
                     AVNLLKEIDPANSTKYQTVLTK"
     gene            complement(1114..>1358)
                     /locus_tag="PK976_07015"
     CDS             complement(1114..>1358)
                     /locus_tag="PK976_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018074703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HRC67673.1"
                     /translation="ILVGFALETNNEKDNALFKLRNKNADIIVLNSTRDKGATFGFAT
                     NRVTIFHKNGDRAQSELLPKTEIAQMILDFIIQHHF"
BASE COUNT          383 a          253 c          291 g          431 t
ORIGIN      
        1 tccaagaata agccccagag ctccgagtaa gatagatttt cctgcaccgg tttcgcctgt
       61 aataatattg aatcccggcc cgggttcgaa ctcgagttct tcgattaaag cataattttt
      121 gacgctcagc ctgcaaagca tagtgttatt cctaattgtt caaaattacg aatagcattt
      181 tgaaattcga attgtggcaa gattattttg tgaggacagt ttggtatttt gtagaattgg
      241 cagggtcgat ttctttgaga agattgactg ctttcacctt atcctgatcg ggagctccgg
      301 tcaatatatt gataaattcg tcacgcttgg catctaggat taattgcaag aggaaagaat
      361 tgggtttatc gcggtaagcc tgacgcataa gttcgaggct ctcgataata ttcgttctgc
      421 cctgatccac attttcggcc atcaagtcga gtccctgacg gtgatattga taaatgaact
      481 gcctaatttt attataactt gtattgttga aattctctgc cagccaataa cgattgcgca
      541 tgctttcgaa cgatttccat cctttttgtg gaaggttctg ggccatgttg acaatggtct
      601 gagctttttc ataaaagggg gttcctccat ataacgaaaa cgaatcgaaa tcgagaccaa
      661 taaggatgta ggcgtaataa gccaatatag ccgttagatt cgaagtaatg gcactttcct
      721 gaaaatcaag aggttcgaat tcgacataat tgaattgaat atctttatct acatgattga
      781 gtaatatgga attgtaactg cttttatata ctggcctgcg gagaaccaca ttcatggttc
      841 ctttgaattc atctacagac agcctttcag agatttgaat gagcatggtg cattcgatac
      901 gttcctccaa tttaaattga tagttggtcc atcgtttgtt gttcatgaat tcgaacaaag
      961 ccgtttgcaa ggtgttatag acctgacggt cggtgccttc tacctgacgt gtcgagacag
     1021 aaatctggca atcgagctcc tgaccaattg aagcaatgga taaaagtaaa atgccgataa
     1081 aaattccaaa acgacgcatt tgacgcaata gttttaaaaa tgatgttgaa tgataaaatc
     1141 gagaatcatt tgagcaattt ctgttttggg tagaagttct gattgtgccc tatcaccatt
     1201 tttatgaaag atagttacac gattggtggc aaacccaaag gttgcccctt tgtcgcgtgt
     1261 cgagttcaaa acgatgatat cggcattttt atttcttagt ttgaagaggg cattgtcttt
     1321 ttcgttgttt gtttcgagag caaaccccac taggatct
//
LOCUS       DAORPD010000584         1358 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_19602_length_1358_cov_1.946278, whole genome shotgun sequence.
ACCESSION   DAORPD010000584 DAORPD010000000
VERSION     DAORPD010000584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19602_length_1358_cov_1.946278"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="PK976_07020"
     CDS             complement(<1..553)
                     /locus_tag="PK976_07020"
                     /inference="COORDINATES: protein motif:HMM:NF014862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HRC67674.1"
                     /translation="MEAKFSQRVKDVLSYSREEAIRLGNDYIGVEHFFLGMVREGEGM
                     AVKIMQYYGADLNEMKAMIESSILNTSSRPVNTDNIPLIRQAERALKLTYLEAKDLSS
                     EVIGTEHLLLAILKVGGNLVEKALQKYDIGYDEIKAEVKLLSTSDENHLSRKDLPYSD
                     LRRGGQSDDDSDDDREFTNLKRNA"
     gene            878..1327
                     /locus_tag="PK976_07025"
                     /pseudo
     CDS             878..1327
                     /locus_tag="PK976_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843967.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
BASE COUNT          401 a          279 c          255 g          423 t
ORIGIN      
        1 cggcattccg tttcaggttc gtaaattctc tgtcgtcgtc ggaatcgtca tccgattggc
       61 caccccgccg taagtcggaa taaggtaaat ctttcctgga tagatgattc tcatcggaag
      121 tcgataacaa ctttacttca gccttgatct catcatatcc aatatcatat ttttgtaaag
      181 ccttttctac aaggttgccc ccaactttca aaatggccag taatagatgc tctgtgccaa
      241 tcacttcgct gcttaagtcc ttagcctcga gataggtgag tttcaaagcg cgttcggctt
      301 gtctgatcaa tggaatatta tccgtattca caggccttga agaagtattt aggatggatg
      361 attctatcat ggccttcatc tcgttaagat cagccccata atattgcatg atcttaactg
      421 ccattccttc gccctcacgt accatcccaa gaaaaaaatg ttcaacccca atatagtcat
      481 tacctaatcg aatcgcttcc tcacgactat atgacaaaac gtctttaact ctttgtgaaa
      541 attttgcttc catataaaac ttaaacctct actttctttt aacaatattc ataagcaatt
      601 gtttcgcaaa attatagctt gttcttttaa aatcgtaatg gttaactgca aattttctac
      661 caaactttca gtatttcaaa aattttttca aaattaatca ttttgatctc tggttttcat
      721 tgtgaaagat tttatttctc agtttggaac tatttatcag gcaaacagat aggtaagtat
      781 gtttcgtacg ggcctcttcg atacgaagaa aatgactaat tttgcacatc ctttttttag
      841 aggataaaaa tcattaaatg aaaagtttag aaaattaatg gaagaaaaac aaggaaaggt
      901 tattgcggtt aatattgaga accagatgaa atcggcctac atcgattatt cgatgtccgt
      961 cattgtttca agagccttac ccgatgtgag agacggattg aagcccgttc atcgcagaat
     1021 attatatgga atgtatgatt tgggcatact ctccaaccga ccatataaaa aatcggcccg
     1081 tatagtcggt gaagtattgg ggaaatatca tcctcatgga gattcgtcgg tttacgacgc
     1141 catggtgagg atggctcagg attggtcgct tcgttatcca ctgattgatg gtcagggaaa
     1201 tttcggaagt atagaccagg atccaccagc cgccatgcgt tatacagaag cccggcttcg
     1261 caagattgcc gaagaaatgt tggtcgacat cgaaaaagat accgttgatt ttcaaaataa
     1321 tttcgaatga ccccgagaat tgttgcccga cgttgcaa
//
LOCUS       DAORPD010000585         1357 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_19618_length_1357_cov_2.877880, whole genome shotgun sequence.
ACCESSION   DAORPD010000585 DAORPD010000000
VERSION     DAORPD010000585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19618_length_1357_cov_2.877880"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            183..758
                     /locus_tag="PK976_07030"
     CDS             183..758
                     /locus_tag="PK976_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009131087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HRC67675.1"
                     /translation="MKNDIIIAGVGGQGILSIAAIIATAAVNNNLYIKQSEVHGMSQR
                     GGEVQSHLRISSSPIASDLIPTGAADMIISLEPMEALRYIPWLKPDGWIITNTEPFLN
                     IPNYPPIEDVLAEIKKRPHHIMMNADEVARELGSEKAANMVILGAATRCLDIPYEEIE
                     KAIRNFFGRKGEEIVKMNLSALAKGREMALL"
     gene            complement(866..>1357)
                     /locus_tag="PK976_07035"
     CDS             complement(866..>1357)
                     /locus_tag="PK976_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67676.1"
                     /translation="MHSRAKIIEKSYSNPDLYLEQPIIIHMMYSIPNYALIGDSNILF
                     QPLLASGIFQRAQPQLGMNLDIDERIYPFRDRCSRKVEVEETIEIPEGFALLKPDYQI
                     NIIGDASSIKASMQTEGKRISISQVALYNKRIYQAEEWSNFKEVVKAQRKFKDSPLIL
                     KKL"
BASE COUNT          408 a          275 c          298 g          376 t
ORIGIN      
        1 caaacaacat accgagaatg ttcgagccat taaggaagaa ttgtcctacc aaggcgtttc
       61 ggtcatcatt cctacacgtg aatgcataca aacactcaat cgtcgcatgc gcgaaaaatt
      121 caaaaacaag aaaaccgaat agacgatttt aaacattact caaaacttaa acagaattca
      181 atatgaaaaa cgatatcatc atagctggtg tgggcggaca aggtattctt tccatcgcag
      241 ccattattgc cactgccgct gttaataaca acctctacat caaacaatcg gaagttcatg
      301 gaatgagcca gcgcggggga gaagtacagt cacatctgcg tatttcgtct tctcccattg
      361 cctctgactt gatccctact ggagctgccg acatgatcat ttcactcgaa ccgatggaag
      421 ccctccgtta tattccctgg ttaaaaccag atggctggat catcaccaat actgagcctt
      481 tcctgaatat tcccaattat cctcccatcg aggatgttct ggctgaaatc aagaaaaggc
      541 ctcatcacat catgatgaat gccgacgagg tagctcgtga actgggctca gaaaaagcag
      601 ccaatatggt gattctggga gctgcaacac gatgcttgga tattccttac gaagaaatcg
      661 aaaaagctat tcggaacttc ttcggaagaa aaggagaaga aatcgtaaaa atgaacttat
      721 cggctcttgc aaaaggcaga gaaatggcat tattataaat agtggatata aggttggtgg
      781 ttgaaaagag actggaggac gaaagggtaa tctgttttcc aacctccaat caccttctca
      841 cgggcgttct tttatcgagt gatttttaca gttttttcaa aatcaggggt gaatctttga
      901 attttctttg ggcctttaca acttccttaa aattgctcca ttcttcggct tggtagattc
      961 ttttgttgta aagagcaact tgagagatag aaatgcgttt gccttcggtt tgcatggaag
     1021 cctttatact ggatgcatca ccaatgatat ttatctgata atcgggttta aggagtgcaa
     1081 atccttccgg gatctcaatg gtttcttcca cttctacctt gcgcgaacag cggtcgcgga
     1141 aagggtagat acgttcatca atatccagat tcataccaag ttgaggttga gctcgttgga
     1201 aaattccgga agccaataat ggctggaaga ggatgttgga atctccaatg agggcgtagt
     1261 tgggaatgct atacatcatg tgaataataa tgggttgttc cagataaaga tcgggattgg
     1321 aataggattt ttcaatgatt ttagctctgg aatgcat
//
LOCUS       DAORPD010000586         1352 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_19732_length_1352_cov_4.195066, whole genome shotgun sequence.
ACCESSION   DAORPD010000586 DAORPD010000000
VERSION     DAORPD010000586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19732_length_1352_cov_4.195066"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="PK976_07040"
     CDS             <1..293
                     /locus_tag="PK976_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRC67677.1"
                     /translation="EFASWALKRGIAIQYIRPGKPTDNSFIESFNGKFRNECLNEHWF
                     MSIQESKQVIEEWRIKYNSYRPHSSLGKCTPYEFAMKQRTMVNNETLALQLA"
     gene            complement(638..982)
                     /locus_tag="PK976_07045"
     CDS             complement(638..982)
                     /locus_tag="PK976_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67678.1"
                     /translation="MHLFGTLFALALGLLVLQILVPADDEEPPILFTRNNEGDPLSFP
                     EIPLDQLILQIDLANLFRCLPPGKKSPNKQSIANQVREYHDGNASHVRTSSPESFKTI
                     ESAFGYQNRCGG"
BASE COUNT          387 a          287 c          330 g          348 t
ORIGIN      
        1 gggaatttgc ctcatgggcc ttaaagagag ggatagccat ccagtacatc aggcctggaa
       61 aaccgacgga taattctttt atcgagagct tcaacgggaa gttccggaac gaatgcctaa
      121 acgaacattg gttcatgagt attcaggaaa gcaaacaggt cattgaagaa tggaggatca
      181 aatataactc gtataggccg cacagttctc tcgggaaatg taccccttat gagtttgcca
      241 tgaaacaaag gaccatggta aataacgaaa cactcgcctt acaattggcc taaagaatgg
      301 ggtaaggcca gaactacatg cttataagcg acatccagaa acattttttg gagtgccctt
      361 gcccttacag aaaaaagcta atacgccaat ggaccttttc gatttctttc atgattcaac
      421 gaggaagatg ccacaagaga aaatacttga gcaaatgaag ggtgccctcg acattatcca
      481 gttacgagag ctctcttatg aggaattagc ttttacttat gctgagagat gtacatattc
      541 actgctcaaa cgagtttagg gttgattatc atcccaatgt tgtagcgatc gggaagccaa
      601 gtgatgttgc caagccattt atcccaccat cctataccta tccgccacat cggttttggt
      661 aaccaaaggc actctcaatg gtcttgaaac tttctggact gcttgtcctt acgtggctgg
      721 cattgccatc gtggtattcg cgaacctgat tggctatgct ttgcttgttc ggagatttct
      781 tcccaggggg caggcatcgg aagaggttgg caaggtcgat ctggaggatc aactgatcaa
      841 gcggaatctc agggaaggag aggggatctc cctcattatt ccgggtgaaa agaatggggg
      901 gttcttcatc gtcggccggg acgagtattt ggagaaccag taggcccaaa gcgagcgcaa
      961 atagtgtccc gaaaaggtgc atccaatctg gcttatcatg ttacggtatc cgttggatgg
     1021 ataccttata aaaccacaat caggatggga gtaatcggct atcagccatg ctcctgataa
     1081 ctattcataa tatggcaggc aaacatcggt gatatgttga tgaatctgca aaaattcaga
     1141 gataggatat tagcctcgga ttgaaaacgc cccttgaata tgccgaggca gagcttggtg
     1201 cccgggaagt cgaagagctt ttgtatcgga ttgaatacca cttgctatca tgaacaagta
     1261 tcactttggt agcaccatgt ccggaaatcc tcatgaggcc taatggtaca ggggtgcggt
     1321 ttcctccacg ttccatatac aaattgtttt aa
//
LOCUS       DAORPD010000587         1352 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_19740_length_1352_cov_2.284503, whole genome shotgun sequence.
ACCESSION   DAORPD010000587 DAORPD010000000
VERSION     DAORPD010000587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19740_length_1352_cov_2.284503"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1352
                     /locus_tag="PK976_07050"
     CDS             <1..>1352
                     /locus_tag="PK976_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67679.1"
                     /translation="VPSGLQCPNIEIRLNGSESGVSYTLWHNGIMGPTMPGTGTLGFI
                     SFGHQTDPGIYKIEAVNNTTGCSSFMLGSVIIQIPPAIYNVIPSGILCANQEVMLSHS
                     DVGIMYQLRLFDTINISSPLAGNGDTLNFGHQSIPGIYRVYAYDPISLCGAWMNNSAY
                     LDSIPQIFSITPIGDTCGSIEIGLNGSQPNFTYYLYRDNIEPPIDSAIGNGSAISFGI
                     YYTSGLYTIKALDENRFCYTEMYGSLQLFQTPYTYNLLPVGNLCVGVSLTLDGSELGT
                     EYQLIKDDSIYINPPILGSGSSISFGIINTPGNYKVIARNLLTSCSSFMNGTAAIHAL
                     PTVYTLSPTGDHCSGTEITLNSSQIGVFYHLNISPPQPGFPLILEGTGDQLNFGVQNS
                     EGTYTITAYGSFGCSNMMQGNTIIHSRPLAYNLIPQGNSCTPVSIGLDNSQLGIIYHL
                     "
BASE COUNT          396 a          268 c          239 g          449 t
ORIGIN      
        1 ggttccttca ggccttcaat gccctaatat tgaaatccgt ttgaacggta gcgaatccgg
       61 agtaagttat actttatggc ataatggtat tatgggtccc actatgcctg ggactggaac
      121 acttggtttt attagctttg gccatcaaac cgaccccgga atttataaaa tcgaagctgt
      181 aaataataca acgggttgtt cttcctttat gcttggctcg gtaatcatcc aaatccctcc
      241 tgctatctat aatgtaattc catcaggaat tttgtgtgca aatcaggaag ttatgttgag
      301 tcattctgac gttggtatta tgtatcaact acggcttttc gatactatta atatatcttc
      361 acccttagcg ggaaatggtg atactttaaa ttttggccat caatcaattc ctggaattta
      421 ccgagtttat gcctatgatc ccatctccct ttgtggagca tggatgaaca attctgctta
      481 tttagacagt attccacaaa ttttcagtat aacacccata ggagatactt gtgggagtat
      541 tgaaattggc ctaaatggga gccaacctaa ttttacttat tatctttata gagataatat
      601 agaaccacct attgattcag ccataggaaa tggatcagct atttcttttg gcatttatta
      661 tacctcggga ttatatacca taaaagcact cgatgaaaat cgattttgtt atacagaaat
      721 gtatggctcg ttacaattat tccaaacacc ttatacctat aatcttttac ctgttggaaa
      781 tctatgtgta ggcgtttcgc ttacattaga tggttcagaa ttagggacag aatatcaact
      841 aataaaagat gattcaattt atatcaatcc tcccattttg ggaagcggca gttcaatctc
      901 ctttggaata atcaacactc caggtaatta taaagtaata gccagaaatc ttcttacttc
      961 ttgttcttcc tttatgaatg gtactgctgc tattcacgca ttgcctacag tttataccct
     1021 gtcaccaaca ggtgatcatt gttctggtac tgaaataaca cttaattcat cgcagatagg
     1081 ggtattttat catttaaaca tatcccctcc tcagcctggt tttccattaa ttttagaagg
     1141 cacgggagat cagttgaatt ttggagtaca aaatagtgaa ggaacttata ccatcacagc
     1201 ttatggaagc tttggttgca gcaatatgat gcaaggaaac actattattc attcccgacc
     1261 tcttgcctat aacctgattc ctcaaggaaa tagctgcacg ccagtttcca tagggctcga
     1321 caatagtcaa cttggaatta tctaccattt at
//
LOCUS       DAORPD010000588         1349 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_19826_length_1349_cov_2.901082, whole genome shotgun sequence.
ACCESSION   DAORPD010000588 DAORPD010000000
VERSION     DAORPD010000588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19826_length_1349_cov_2.901082"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="PK976_07055"
     CDS             complement(<1..477)
                     /locus_tag="PK976_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005834007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HRC67680.1"
                     /translation="MGYLFTSESVSEGHPDKVADQISDALLDEFLRWDPDSKVAVETF
                     VTTGLVVVGGEVKTSGYVDVQEIARQTIRRLGYTKAEYRFDSDSCGVISAIHEQSPDI
                     FQGVAKSNPLEQGAGDQGMMFGYACKEMDNYMPMPIELAHILVQQLAAFRREGKEMP"
     gene            complement(798..>1349)
                     /locus_tag="PK976_07060"
     CDS             complement(798..>1349)
                     /locus_tag="PK976_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRC67681.1"
                     /translation="GLEPEISKTIGKLSKGFRQRVGLAQALIPDPEVLILDEPTSGLD
                     PNQIIEIREVIKKASANKTIIFSTHIMQEVEALCQRVIIINKGKIVADDLTANLSNLV
                     SSSETILAGFDRKVEIHLIRQINGVIAVKAKENNVFEIKAKPNTDIRPALFKFAVDHH
                     FTVLSLQQSEKSLEEIFHEITQK"
BASE COUNT          387 a          284 c          259 g          419 t
ORIGIN      
        1 gggcatttct ttaccttccc ttcgaaatgc tgccagttgc tgtacaagaa tatgcgccag
       61 ttcaattggc atgggcatat aattgtccat ttccttgcaa gcatagccaa acatcattcc
      121 ttggtcgcca gcaccctgct caagtgggtt tgatttggct actccctgaa aaatatcagg
      181 tgattgctca tgaattgcac taatcactcc gcaagagtcg gaatcaaaac ggtactcggc
      241 tttggtatat cccaaacggc gaatagtttg ccgtgctatc tcctggacat ccacataacc
      301 cgaggttttt acttcgcctc ccaccactac caaaccggta gttacaaagg tttcgacggc
      361 aactttcgaa tcgggatccc aacgtaaaaa ttcatcgagc aatgcatccg aaatttgatc
      421 ggctacctta tcgggatggc cttctgacac tgattctgaa gtaaataaat atcccattat
      481 gtctttaatt tattggttga acatgtaaat ggggaaattg attgagaagg aaaaaaatat
      541 tgttttagca tttttttctg tggttgcaat caatcaaatc tctccacttg cttttaataa
      601 atgaactgca aaaatagaat aaatctttcg acaaaaaata tcccaaccaa agattttact
      661 ctttctaaac ataaaatatt gatatatggg gaaatatcct attcatcgaa aaaacacaaa
      721 ttcaaaagtt gttgacttgt gctgtaaccc tgaaaagaaa agggtgaatt taaaatcatc
      781 taactaatgg atgagaatta cttttgggta atttcatgaa atatttcttc gagactcttt
      841 tcgctctgtt gcaatgataa caccgtaaaa tgatgatcga cggcaaactt gaacagtgca
      901 ggccgaatat ccgtattggg tttcgccttt atttcaaaca cattgttttc ttttgccttc
      961 accgcaatta ctccattgat ttgacgaatg aggtgaattt cgacttttcg gtcgaatccg
     1021 gcaaggatcg tttcactgct cgatacaaga ttcgacagat tagccgtaag atcatcggct
     1081 acaattttgc ctttgttgat gatgatgacc ctctggcata atgcttccac ctcctgcatg
     1141 atgtgtgtgg aaaagatgat ggttttattg gcgcttgcct ttttaatgac ttccctgatt
     1201 tcgataattt gattaggatc gaggcctgaa gtaggttcat ccaggattaa gacttcaggg
     1261 tcgggaatca aagcttgtgc cagtccaact ctctgccgga aacctttcga caattttcca
     1321 atagtcttcg aaatttcggg ttcgagccc
//
LOCUS       DAORPD010000589         1348 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_19865_length_1348_cov_1.887858, whole genome shotgun sequence.
ACCESSION   DAORPD010000589 DAORPD010000000
VERSION     DAORPD010000589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19865_length_1348_cov_1.887858"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="PK976_07065"
     CDS             <1..273
                     /locus_tag="PK976_07065"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC67682.1"
                     /translation="GIVENNSPNFQLYIHPNPNNGNFFVTLNASKSAEIVLCITDNKG
                     NEIYRNTLSSSKIQHYPINLGNPSSGVYLLKVESKTGKSTAKIIVK"
     gene            509..>1348
                     /gene="rlmN"
                     /locus_tag="PK976_07070"
     CDS             509..>1348
                     /gene="rlmN"
                     /locus_tag="PK976_07070"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HRC67683.1"
                     /translation="MQRLYGKTLAELGEIAQALSFPSYTAKQIAQWLYQHHINHIDEM
                     NNLSKTARDKLKERFTLGLSAPVRVQISKDGTKKYLYSLAGGRFIEAAYIPDNDRGTL
                     CVSSQMGCKMGCLFCMTGKQGFQANLTVNEILNQIRCLPEWHELTNIVFMGMGEPLDN
                     IDNLMKSLEILTSDWGYARSPKRITVSTIGIIPTMKTFLENSHCHLAVSLHSPFNEER
                     KKLMPVENVYPINEIVQLLKNYDWTGQRRLSFEYIMFKGLNDSLTHARALLKLLNGLN
                     CRLN"
BASE COUNT          435 a          277 c          235 g          401 t
ORIGIN      
        1 ggtatagtcg aaaataattc tcccaatttc caattatata tacatcccaa ccctaacaat
       61 ggaaatttct ttgttacctt gaatgcatca aaatctgccg agatcgtttt atgtattacc
      121 gacaataaag ggaatgagat ttatcgtaat accttgtctt cttctaagat tcaacattat
      181 cctataaatc ttggaaatcc atcttcgggt gtttatttgt tgaaggttga aagtaaaact
      241 ggcaagtcca cagccaaaat tatagttaaa taaataacca ttcttttcaa tgcagcctcg
      301 tcagttttta attgatgagg ctgcttcacg tttagaatat aatttatgtt cattcaaccc
      361 ttaggagttt ctacatacaa ataaatttat tacttatttt tgcgaatatt accgaaatgg
      421 atttttgcag cacaagatac cctaattcat tgcaaaagcc ctaaatttaa ataacccagc
      481 tcggaaatat atttattcac tccaggatat gcaacgcctt tatggaaaaa cattggccga
      541 attgggcgaa attgcccaag cattgagctt tccttcctac acagcaaaac aaatagccca
      601 atggctttat caacatcaca taaaccacat cgacgagatg aataatcttt cgaaaaccgc
      661 tcgtgataaa ttaaaggaaa ggtttacttt gggactctcg gctcctgtac gagttcaaat
      721 ttcgaaggat gggaccaaaa aatacttgta tagcttggca ggagggcgat ttattgaagc
      781 agcttatatt cccgataatg accgtggtac gctttgcgtt tcctctcaga tgggttgcaa
      841 gatgggttgt ttattctgca tgacgggaaa acaaggtttt caggcaaacc taactgtaaa
      901 tgaaatcctg aatcaaatta gatgcctacc cgaatggcat gaactgacca atattgtgtt
      961 catgggaatg ggcgaacctc tcgataatat agataattta atgaaaagtc ttgaaatact
     1021 tacctccgac tggggttatg ccagaagccc gaagcgtatc accgtttcga caataggaat
     1081 aatccctacc atgaaaactt tcctcgaaaa cagccactgc catctagctg tcagcctcca
     1141 ttctcctttc aatgaagaac gtaaaaaact catgcctgtc gaaaatgtct accctatcaa
     1201 tgaaatcgtt caacttctca aaaattacga ttggacaggt cagcgcagac tgtcattcga
     1261 atatatcatg ttcaaaggat taaacgattc acttactcat gccagagccc tgttaaaatt
     1321 gctcaatgga ctgaattgcc gtttgaat
//
LOCUS       DAORPD010000590         1346 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_19921_length_1346_cov_2.155693, whole genome shotgun sequence.
ACCESSION   DAORPD010000590 DAORPD010000000
VERSION     DAORPD010000590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19921_length_1346_cov_2.155693"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="PK976_07075"
     CDS             <1..485
                     /locus_tag="PK976_07075"
                     /inference="COORDINATES: protein motif:HMM:NF014907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HRC67684.1"
                     /translation="EKETIQKLQRLLAIQGKYSVATLHKKLGKIMWENVGMARDEARL
                     KQAIEAIRSLKQEFWKEVYVPGSLTGVNDELLKANRLADYLELAELIAHDALLRNESC
                     GCHFRVEYQSEDGEALRNDEEYKYVSCWEYQGEGVEPQLHKEPLEYEFVELKSRNYKV
                     "
     gene            516..1301
                     /locus_tag="PK976_07080"
     CDS             516..1301
                     /locus_tag="PK976_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HRC67685.1"
                     /translation="MNFTLKIWRQENSKAKGRFKTYKIENISPDCSFLEMLDILNEKL
                     IHDKIELPVDPVAFDHDCREGICGMCSLYINGHPHGPEQGTTVCQVHMRKFQDGDAIV
                     IEPWRAKAFPIIKDLMVDRSAFDKIIQEGGYISASTGGVPDANSILVSKEYADMAMDA
                     AACIGCGACVAACKNASAMLFVAAKVSQFALLPQGHPEAKRRVQAMVSKMDELGFGSC
                     TNTYACEAECPKNISVEHIARMNREFLKSKLTHAEKKRIGGFG"
BASE COUNT          420 a          259 c          306 g          361 t
ORIGIN      
        1 ccgaaaaaga aacgattcaa aagctgcaac gtttgcttgc tattcaggga aaatactcgg
       61 tcgctacact ccataagaaa ttgggtaaga ttatgtggga aaatgttgga atggctcgcg
      121 atgaggctag gcttaagcag gccatagagg caattcgctc attaaaacaa gaattctgga
      181 aagaagtata tgtgcccggc tcccttacag gagtaaatga tgaacttttg aaagccaatc
      241 gcttggccga ttaccttgaa ctcgcagagc tgatcgccca cgatgcctta cttagaaatg
      301 aatcatgcgg atgccatttc agagtggaat atcaatctga agatggagaa gcgctccgaa
      361 atgacgaaga atataaatat gtatcctgct gggaatatca aggagaagga gttgaacccc
      421 aacttcataa ggaacccctc gaatatgagt ttgttgaatt gaaatcccgt aattataaag
      481 tttaactcat ttcctgaacc taaaaattta acattatgaa ctttacattg aaaatctggc
      541 gtcaggaaaa ttccaaagcc aaaggaagat tcaagacata taaaattgag aatatctcac
      601 ccgattgttc ttttctcgag atgctcgata tcttgaacga aaaattgatc catgataaaa
      661 tagagcttcc ggtcgatcct gttgccttcg atcatgactg ccgtgaagga atttgcggaa
      721 tgtgcagcct ttatatcaat ggacatcctc atggacctga acagggcact acagtttgtc
      781 aggttcacat gcggaaattc caggatggag acgccatcgt aatagaaccc tggcgagcaa
      841 aggctttccc aataattaaa gatttaatgg tagatcgaag cgctttcgat aagattattc
      901 aagaaggagg ttatatatcg gcttctactg gtggtgtgcc tgatgcaaat tccattcttg
      961 tttcgaaaga atatgccgat atggccatgg atgcggctgc atgtattggg tgtggagcat
     1021 gtgtggcagc atgtaagaat gcctcggcca tgttgtttgt tgccgcaaag gtttctcagt
     1081 ttgccctcct tccacaaggc catcctgaag caaaaagaag agtgcaggca atggtctcca
     1141 aaatggatga attaggattc ggaagctgta ccaatactta tgcttgtgaa gccgaatgcc
     1201 ccaagaacat ttcggtagaa catatcgctc gcatgaatag agaatttttg aaatcgaaac
     1261 ttactcatgc cgaaaagaaa agaattggag gctttggctg ataatgtttt tttgattact
     1321 acccgtctta accaatacaa ggttgt
//
LOCUS       DAORPD010000591         1343 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_20012_length_1343_cov_2.333851, whole genome shotgun sequence.
ACCESSION   DAORPD010000591 DAORPD010000000
VERSION     DAORPD010000591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20012_length_1343_cov_2.333851"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1343)
                     /locus_tag="PK976_07085"
     CDS             complement(<1..>1343)
                     /locus_tag="PK976_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HRC67686.1"
                     /translation="HIKKYMAENNISFYIINATAIAEEIGLGHRTNTIMQSAFFKVSK
                     VISYDLAVEKMKEMIKKSYGRQGEDIVRMNYAAVDRGGDVIKVEIPAEWANIQIEVEK
                     DERPLPEFIKKVVEPMNAQKGDDLPVSVFLDREDGTFPAGTTKYEKRGIAVTVPEWIP
                     ENCIQCNQCAYICPHAAIRPFLLTAEEAASAPEGTTTLKAVGKGMGEMKYRLQVSVLD
                     CTGCGNCVEECPSKNKALIMKPLDNQHSEISRWEFMDTKIGYKEQYFDKTKNVKNSQF
                     AQPLFEFSGACSGCGETPYIKLITQLYGERMIIANATGCSSIYGGSAPSTPYTSNYDS
                     GHGVAWANSLFEDNAEYGFGIATGIQKLRNRIAMRMKEAMEKDISPELKEAYGLWIEG
                     MNDYDKSNEATKLMIPLLEKADDEISKEILALHQYLIKKSIWVIGGDGWAYDIGY"
BASE COUNT          329 a          310 c          272 g          432 t
ORIGIN      
        1 atagccaatg tcgtaagccc aaccatcgcc tccaatcacc cagatggatt tcttgatgag
       61 atattgatgg agtgccagaa tttctttcga aatctcgtca tcggcctttt ccagcaaagg
      121 aatcataagt tttgtggctt cattcgactt atcataatcg ttcatgcctt ctatccataa
      181 accataagcc tccttcagtt cgggactgat atctttttcc atggcttctt tcatcctcat
      241 ggcaatccgg ttgcgaagct tctggattcc tgtggcaatt ccaaatccat attctgcatt
      301 gtcttcgaag agcgaattag cccaggcaac accatgtcct gaatcataat tcgatgtgta
      361 aggagtactt ggagccgatc ctccatagat agaagaacac cctgtagcat tggcgatgat
      421 cattctttcg ccataaagtt gagtaatcag tttaatgtat ggagtttcac cacatcctga
      481 gcatgctcct gagaattcga ataaaggttg ggcaaactgg ctgttcttta cgttcttggt
      541 tttatcgaaa tattgctctt tatatccaat tttggtatcc atgaattccc agcggctgat
      601 ttctgaatgc tggttatcga gaggtttcat tattagggct ttgttcttcg aaggacattc
      661 ttcgacgcaa ttaccgcaac ccgtgcaatc gagcacactc acctgaaggc ggtatttcat
      721 ttcacccatt cccttgccta cagccttaag ggttgtggtt ccttcgggtg cacttgcagc
      781 ttcttcagca gtcaataaaa agggacggat ggcagcatgt ggacaaatat atgcacactg
      841 gttacattga atgcagtttt cggggatcca ctcgggcacg gttacggcta tgccgcgttt
      901 ttcgtatttg gttgtaccgg ccgggaaagt accatcttct ctatctaaga atacacttac
      961 gggaaggtcg tctccctttt gggcattcat aggttctacc acttttttga tgaattcagg
     1021 cagaggacgt tcgtctttct cgacttcaat ttgaatgttt gcccattcag caggtatttc
     1081 tactttaatg acgtctccac cgcggtcaac ggctgcataa ttcatcctga ctatatcctc
     1141 tccctgtcgg ccatacgact ttttgatcat ttccttcatc ttctcaacag ccagatcgta
     1201 agaaatgacc ttggatacct taaagaaagc gctttgcatg atggtattgg tacgatgtcc
     1261 taatccaatt tcttcggcaa ttgccgtagc attaatgata taaaaagaga tattattttc
     1321 ggccatatat tttttaatat ggt
//
LOCUS       DAORPD010000592         1342 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_20041_length_1342_cov_2.070707, whole genome shotgun sequence.
ACCESSION   DAORPD010000592 DAORPD010000000
VERSION     DAORPD010000592.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20041_length_1342_cov_2.070707"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="PK976_07090"
     CDS             <1..671
                     /locus_tag="PK976_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67687.1"
                     /translation="AIFSVGGIGMAMSAPALLGVTAAGVFVGTGYYLISNWWSGIKKN
                     GQDDKLGEIVVASGKIGDPIPTTMLSNGASLTVAISGYAPVYIPAFILPSQGIKKTLE
                     ITPIKLEDATPTTKVEVCTLEEEFTSENCEDVLYVTATPSPIDPGPGEGVTVTATIFP
                     PIAGCQISFSIVGTDGYTKSETHETDANGKASFFIPGGAEGVFDIVTITTSNGHTYTV
                     TYTF"
     gene            771..>1342
                     /locus_tag="PK976_07095"
     CDS             771..>1342
                     /locus_tag="PK976_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67688.1"
                     /translation="MKTRHLLRKTFIIGLVVFSLPRLYSQNGFTENKGQWDSNVLFLL
                     QPQADVAVFIEAGAITFNIQLLRHDETPNFENISAKTNHEGVKGHAFRIIFEDADFSN
                     ITALRPKPELLNYFLGKNPEKWSTGVKIFNELYIYDAYKNIDLKIISDIGGIKYEFII
                     HRGGRVKDIAIRYEGLENISLSNGQLILSTS"
BASE COUNT          419 a          257 c          259 g          407 t
ORIGIN      
        1 cagctatttt tagtgtaggg ggaatcggta tggccatgtc tgcgcctgcc ctgctgggag
       61 taacagccgc aggagttttt gttggtacgg gatattattt gatatccaat tggtggtcag
      121 gaattaagaa aaatggacag gatgacaaat taggcgagat tgtagtggct tcaggaaaaa
      181 ttggggatcc tattcctaca actatgctca gtaatggcgc cagtctaact gttgctattt
      241 caggttacgc tccggtttat attccagcat tcatcctacc ttcgcaagga ataaagaaaa
      301 ccctcgaaat tactccgatt aaattagaag acgccactcc tacaacaaag gtagaagtat
      361 gcaccctaga agaagaattt acatctgaaa actgtgaaga tgtactttat gttactgcca
      421 caccctcacc tatcgaccca ggcccaggcg aaggcgttac agtaactgcc accattttcc
      481 ctcccatagc aggatgccaa atttcattca gtattgttgg aacagatgga tacactaaaa
      541 gcgaaacgca tgaaaccgat gctaacggaa aagcatcttt cttcatccct ggtggcgccg
      601 aaggagtttt tgatatcgtg accattacta cttcgaatgg ccatacttat actgtaactt
      661 atacatttta aaattatcta attgtcttat tataaaaaag ccttcgttgc cgaaggcttt
      721 tttattgcct tgttcacttg ttcaattatt taacttatat ttgtttctct atgaaaacaa
      781 ggcatttatt aagaaagaca tttattattg ggctggtggt ttttagtctt cccagattat
      841 attcccaaaa tggcttcact gagaataaag gacaatggga ttctaatgtt ctcttcttgt
      901 tgcaacccca agcagatgtc gcagtattta ttgaagcagg tgccataacc ttcaatattc
      961 aactattacg gcatgacgaa acaccaaatt ttgaaaatat tagcgctaaa actaatcacg
     1021 aaggtgttaa aggacatgca tttcgaatta ttttcgaaga tgctgatttt tcaaatatca
     1081 ctgctttaag accaaaaccc gaactactca attattttct tggaaaaaat cccgaaaaat
     1141 ggagtaccgg tgtaaaaatc tttaatgaat tatacatcta tgatgcatac aagaatatag
     1201 accttaaaat tatttccgat attggaggta ttaaatacga attcatcatt catcgaggag
     1261 ggcgggtcaa agatattgcc attcggtatg agggattgga gaatatatca ttgagcaatg
     1321 gccaacttat tttgagtaca tc
//
LOCUS       DAORPD010000593         1342 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_20043_length_1342_cov_2.038850, whole genome shotgun sequence.
ACCESSION   DAORPD010000593 DAORPD010000000
VERSION     DAORPD010000593.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20043_length_1342_cov_2.038850"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(333..1214)
                     /locus_tag="PK976_07100"
     CDS             complement(333..1214)
                     /locus_tag="PK976_07100"
                     /EC_number="1.8.4.11"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methionine sulfoxide reductase B/A
                     protein"
                     /protein_id="HRC67689.1"
                     /translation="MNDSTSRDTSKQYNALSPEEERIIVHKGTERPFSGKYYDFWEEG
                     TYVCKRCNASLFRSAAKFDAGCGWPSFDEEIPGAVIRQPDTDENRTEILCAKCGAHLG
                     HVFTGEDFTPKNIRHCVNSLSLRFIPQKSSQTSDTAIFAGGCFWGVEYHFKNVKGIIS
                     TRAGYTGGTLSHPTYQQVCSGSTGHAEAVEVIFDPEQLSFEDLAKLFFEIHDPTQLNR
                     QGPDVGLQYRSAIFYFDDEQKAITEKLISILKSKGFDVLTELKPASTFWPAEDYHQDY
                     YEKTSKKPYCHIYTKRF"
BASE COUNT          410 a          264 c          269 g          399 t
ORIGIN      
        1 gttgagaaaa tagaacagaa gcagcatcat caggctgggc taatttcaga taacccctcc
       61 ttacctcgaa tcttggcctt aattcggcat cgaccctaaa ttcatcttgg gctgcaactc
      121 tcgctaatcc aataaaaata aataatccga aaaacaagag tttttgcttc atacagatcg
      181 agtattaatg aatattgtcc tcatttatag acaagatatc tcacctttcc ttactatatc
      241 gggcaagaaa ttgtgattta tagtcaaaat gagataatag aatcaatagg gctcaaaaat
      301 acgggaaaat tgtcaggtta ttcccatcta cttcaaaatc gtttggtata aatatgacaa
      361 taaggctttt tgctggtttt ttcgtaataa tcctgatgat aatcttcggc tggccagaag
      421 gttgaagcag gtttcagctc tgtaagcacg tcaaatcctt tcgatttcag aatagagata
      481 agtttttcgg taatggcttt ttgttcgtca tcgaaataaa aaatagccga gcggtattga
      541 agtcccacat ctggtccctg tcggttcagt tgcgtgggat catggatttc gaaaaataat
      601 ttggctaagt cttcaaaaga aagctgttca ggatcgaaga ttacttcaac agcctcggca
      661 tggccagtac ttcccgaaca tacctgctgg taggttggat ggctgagtgt acctccagtg
      721 tatccagctc tagttgaaat tattcctttt acatttttga aatgatattc tacgccccaa
      781 aagcagccac cagcaaaaat ggctgtgtca gaggtttgag acgatttttg aggaataaat
      841 cgaagcgaaa gactattgac acaatgacgg atatttttag gcgtaaaatc ctcacccgta
      901 aatacatgcc cgagatgcgc tccacacttc gcacataaaa tttcggtacg attttcatcg
      961 gtatcgggtt gtcgaatgac ggctccggga atttcttcat caaagcttgg ccagccacaa
     1021 ccagcatcga attttgctgc cgaacgaaac aaagaagcat tgcatctttt gcatacataa
     1081 gtcccttctt cccaaaaatc gtaatatttc ccactgaagg gtctttcggt gcctttgtgc
     1141 actataattc tttcttcctc gggagaaagg gcgttatact gttttgaagt atccctactg
     1201 gtagagtcat tcatattggt atggctagag ttacaagata gtgagagtaa gagcatagac
     1261 aaaattactg gaaatgaatt catttcgagg ttttctaaaa gaacaaaaaa gttggcagaa
     1321 tgtttatgac ttttcaaaat ga
//
LOCUS       DAORPD010000594         1339 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_20118_length_1339_cov_2.225857, whole genome shotgun sequence.
ACCESSION   DAORPD010000594 DAORPD010000000
VERSION     DAORPD010000594.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20118_length_1339_cov_2.225857"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..174)
                     /locus_tag="PK976_07105"
     CDS             complement(<1..174)
                     /locus_tag="PK976_07105"
                     /inference="COORDINATES: protein motif:HMM:NF012601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HRC67690.1"
                     /translation="MDEQKNIRPSWDEYFIEVANTVANRATCDRGRSGCVIVRDKQIL
                     VTGYVGSPVGFPHC"
     gene            complement(179..913)
                     /gene="gldF"
                     /locus_tag="PK976_07110"
     CDS             complement(179..913)
                     /gene="gldF"
                     /locus_tag="PK976_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     permease subunit GldF"
                     /protein_id="HRC67691.1"
                     /translation="MFTLFKKEINAFFNSIIGYIVLAVFLVVNGLFLWVFPLDFNILD
                     FGYANMDGLFILTPFVFLFLIPAITMRTFADEKSKGTIETLFTKPLTDMQIILAKYFA
                     SFLLVVLSLLPTLIYFFSVYQLGMPRGNLDSGAIWGSYIGLLFLGASFTAIGVFSSSI
                     TDNQIIAFIVGAGLCAFVYLGFEFIYGLALFGKIDLFIKSLGMNAHYASMSRGVIDTR
                     DVIYFFSVIVIFLLLTQFVLERRKWK"
     gene            1145..>1339
                     /locus_tag="PK976_07115"
     CDS             1145..>1339
                     /locus_tag="PK976_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005820352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HRC67692.1"
                     /translation="MKAEIITIGDEILIGQIIDTNSAWIANELNLIGIDVCRMISIAD
                     QADEIIRAVDDSFQRVDVIVM"
BASE COUNT          496 a          255 c          253 g          335 t
ORIGIN      
        1 gcaatgagga aaacctacag gtgagcctac ataacctgta accagaattt gtttgtcgcg
       61 tacgatgaca catcccgaac ggcctctgtc gcaagtagcc cgattagcta ccgtattggc
      121 aacttctatg aaatattcgt cccagcttgg acgtatattc ttctgttcat ccataagatt
      181 atttccattt tcgacgttca agaacaaatt gcgtaagaag taaaaaaatt acgattacac
      241 tgaaaaaata gattacatca cgagtatcga tgacacctcg gctcatcgaa gcataatgag
      301 cattcatacc cagagatttg ataaataaat cgattttccc aaacaaagcc aaaccataga
      361 taaactcgaa accaaggtag acaaatgcac ataaaccagc cccgactata aaggcaataa
      421 tttgattgtc ggtgatgctc gatgaaaata cgccaattgc cgtaaaagat gcacctaaaa
      481 acaataaacc aatataggaa ccccagatgg ctccactatc caaattgccc ctgggcatac
      541 ccagctgata taccgaaaag aaatagataa gtgtgggcaa aagggaaaga acaactaata
      601 agaaacttgc aaaatatttt gctaaaataa tttgcatgtc cgtaagcggt tttgtaaata
      661 aggtttcgat ggtgcccttg cttttctcat cggcaaatgt acgcatggta atagccggaa
      721 tcaggaatag aaagacaaag ggtgtcaaaa tgaataagcc atccatattg gcataaccaa
      781 aatcaagaat attgaaatcg agcggaaata cccataaaaa caagccattc accaccagaa
      841 agacggctaa aacaatgtag ccgatgatgg aattgaagaa agcattgatt tcctttttaa
      901 acaatgtaaa cataaacttt cagcctctta aagtattaca cgattccttt ctcgattggc
      961 aggattcaat taatcagtca ggtgcaaaac tataaaattt gcataagtaa agaaaaaatg
     1021 tatccattta atgaccaaga tccgacacaa cagaaatgaa tgggaggatt gacttgttta
     1081 gtgaaaaaca gcaatatgat aaagcattgt agatataaat ttgcacatcg aattataatg
     1141 aaccatgaaa gcagaaatta ttaccatagg cgatgaaata ttgataggtc agatcatcga
     1201 cacgaattcg gcatggatag ctaacgaatt gaatttgatt ggtatagacg tttgtcgcat
     1261 gataagtata gccgaccagg cagatgaaat cattcgagcc gtcgacgata gtttccagcg
     1321 agtcgatgta attgttatg
//
LOCUS       DAORPD010000595         1337 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_20180_length_1337_cov_2.328393, whole genome shotgun sequence.
ACCESSION   DAORPD010000595 DAORPD010000000
VERSION     DAORPD010000595.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20180_length_1337_cov_2.328393"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="PK976_07120"
     CDS             complement(<1..405)
                     /locus_tag="PK976_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007210510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid aminotransferase"
                     /protein_id="HRC67693.1"
                     /translation="MPDIKWSELPFGYLKTDFNVRAYYRNGAWSELEVSDSEYINLHM
                     AATALHYGQEAFEGMKAFRGADGKIRVFRWIENAKRLAYSARGIMMAEVPIELFKEAL
                     WKVIKLNERFIPPYGTGASLYIRPLLIGSGAQV"
     gene            729..>1337
                     /locus_tag="PK976_07125"
     CDS             729..>1337
                     /locus_tag="PK976_07125"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HRC67694.1"
                     /translation="MCSICLFSCNHHEIITIGVSRLNPAYHQWLLQIDSNLKIIDLYS
                     LPFDSVMLALDECDGLLITGGEDIYPLYYGKEFDTSICEAFDRHRDTLEFMAFEKALH
                     RGLPIFGICRGLQLINVALGGSLIADIPSELGTEVIHRCDNRPDCLHPVTVNGNSLLY
                     SITGAKEVYVNSIHHQGIDRLADELKPAAFAPDQLIEVIEWRD"
BASE COUNT          380 a          270 c          245 g          442 t
ORIGIN      
        1 tacctgagct ccactaccaa tgagcaatgg ccggatgtat aatgatgctc cagtaccata
       61 gggtggaatg aaacgttcat tcaatttaat tactttccac aaagcttctt tgaaaagctc
      121 gattggaact tcggccatca ttattccacg agccgaataa gccaaacgct tggcattttc
      181 gatccacctg aataccctga tcttaccatc agcaccacga aaagccttca ttccctcgaa
      241 ggcttcctgc ccgtaatgta aagctgtggc agccatatgc aaattaatgt attccgaatc
      301 ggatacctcc aattcactcc atgcgccatt gcgataatag gctctgacgt tgaaatcggt
      361 ttttaaataa ccaaatggaa gttcactcca tttaatgtct ggtatattac tcatgatgta
      421 ttgattttaa gtttccgaaa acgtttgcaa gatacaaata aatatctgaa ccaaggtgca
      481 taatatatct ctcttttctc ttaaattctc gtattctatt ttacttcatc gacttattca
      541 gaaaataatt tttttacatg tagaattttg catatttaac tcgtctaatt tctttcatcc
      601 tatgaatcga aattaggctt gttacccagt tgtttttaat tttgtcaaag cttagaaaca
      661 atagtcaatg aatcgattta tttaatgcta tgaaaaccct tcgcctttca aacggaattt
      721 tattgtttat gtgttcgata tgtttattct catgtaatca tcatgaaatt attaccattg
      781 gtgttagccg gttaaaccct gcttatcatc agtggttatt acaaatcgat tcgaatctta
      841 aaataattga tttatattct ttacctttcg attcggttat gttggccttg gatgagtgtg
      901 acggcctgtt aatcaccggg ggtgaggata tttatccgct ttattatggg aaagaattcg
      961 acacctcgat atgtgaggcc ttcgatcggc atagggatac cctcgaattt atggctttcg
     1021 aaaaagccct gcaccgaggt ttacccatct ttggaatctg cagaggatta cagctaatca
     1081 atgtagcttt agggggaagt ctcattgccg atattccttc tgagctagga accgaagtga
     1141 tccatcgctg cgataatagg ccagattgtc tacatccggt tactgtgaat ggtaattctt
     1201 tattatattc cattacggga gccaaggaag tttatgtaaa ttcaattcat catcaaggta
     1261 tcgacagatt agccgatgaa ctgaaacctg ctgcttttgc tcccgatcag ttaatcgagg
     1321 taatcgaatg gagagac
//
LOCUS       DAORPD010000596         1337 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_20181_length_1337_cov_2.311232, whole genome shotgun sequence.
ACCESSION   DAORPD010000596 DAORPD010000000
VERSION     DAORPD010000596.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20181_length_1337_cov_2.311232"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1337
                     /locus_tag="PK976_07130"
     CDS             <1..>1337
                     /locus_tag="PK976_07130"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HRC67695.1"
                     /translation="FIKFGQILRTRPDFLPPDYIRELEKLQDTVAPIEVKKIQEIIET
                     ELHRPIAQAFKEFDEQPVASASLSQVHRAVLQNGNVVAVKVQKPDIQNTIELDISILE
                     TFANIIEKRFPEGPFYHPMAMVNELKRTIRKELDFLNEAHNFEKFRTNFKDVNCIKIP
                     EVYWDLTTERLLVMEFIDGIKISEITHEKYRSRFDLKKVAHQAAEALLKQILLDGFFH
                     ADPHPGNLFVIPPNTIVMLDVGMTGHLDDQSLLSIAKILRAINHRDFDQIIRGLQELG
                     IVMQEVDKTLLRHDIKELLDIYTDRPFKTISLARMNQDILDIAMRHRLAIPSNFVMMI
                     RAMSIAETIGKQLESDFDILEMVKPFTRKIYLKLYSPRSWKKRTANMLDASVELIEHL
                     PQNIYDLLEKLKEGQFEIHLEHHRLENLANEIKSSSNRIAVSLIIAALIVGSS"
BASE COUNT          416 a          253 c          278 g          390 t
ORIGIN      
        1 ttttatcaag tttggtcaaa tattgaggac ccgtcccgat tttttacctc ccgactatat
       61 ccgtgagttg gaaaagcttc aggatactgt ggcgccgata gaagtaaaaa aaattcaaga
      121 aattatagaa actgagcttc atcgaccaat tgctcaggca ttcaaggaat tcgacgagca
      181 acccgttgct tcggcttcat taagtcaggt ccatagagcc gtattacaga atgggaatgt
      241 tgttgcggta aaagtccaaa aacctgatat tcaaaatact attgaactgg atatttctat
      301 tttggaaact tttgccaaca taatcgagaa acgtttcccg gaaggtccat tttatcatcc
      361 catggctatg gtaaatgaat tgaaaagaac cataagaaaa gaactcgatt ttttgaatga
      421 ggcacataat ttcgaaaaat tcaggactaa tttcaaagat gtcaattgta taaaaattcc
      481 agaagtttat tgggatttaa ctaccgaacg ccttcttgtg atggaattca tcgatgggat
      541 aaagatttcg gagattaccc atgaaaagta tcgctctcgc ttcgatttga aaaaagtggc
      601 tcatcaggcg gcagaggctt tattgaaaca aattctgttg gatggatttt tccatgccga
      661 tccgcatccc ggtaacctct tcgtgattcc acccaatact attgtgatgc tggatgttgg
      721 catgacgggg catttggatg atcaatcact attgagtatt gctaaaattt tacgagccat
      781 caatcataga gattttgacc aaattattag aggtttacag gaattgggaa ttgtaatgca
      841 ggaagtggat aaaaccttgc ttcggcatga tattaaagaa cttctggata tttataccga
      901 ccgacctttc aagacaatca gcctggcacg tatgaatcag gatatcttgg atattgcaat
      961 gcggcatcgt ttggctatac cttccaactt cgtgatgatg atcagagcca tgtcgattgc
     1021 cgaaaccatt ggcaagcagt tagaatctga tttcgacatc ctggagatgg tgaagccatt
     1081 tactaggaaa atttatctta aactctattc cccccgaagc tggaaaaaac gtactgccaa
     1141 tatgttggat gccagtgtcg aactcattga acatttacca caaaatatct atgatctgtt
     1201 ggagaagttg aaagaaggac aattcgagat tcatttggag catcaccgct tagaaaacct
     1261 tgccaacgag attaaaagct cgagcaaccg tattgcagtg agtttgatta ttgcagcctt
     1321 aatcgttggc tcttcaa
//
LOCUS       DAORPD010000597         1336 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_20203_length_1336_cov_2.602654, whole genome shotgun sequence.
ACCESSION   DAORPD010000597 DAORPD010000000
VERSION     DAORPD010000597.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20203_length_1336_cov_2.602654"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="PK976_07135"
     CDS             <1..612
                     /locus_tag="PK976_07135"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRC67696.1"
                     /translation="ALIYGRLERDFPNHPLGEEAKLEYAKLMYQAGEHKWALLHLEIL
                     RGAIHKPVANDAQELYLFIQEALNEDTTGRILHYFGSSELLSIRKQYAMALRTLDSVS
                     MFFGNSWGQDYALLRKARIYKEMREYNIADSIYSTLVRIFPRSLVADDALLELASLRE
                     ITHQPEKAYKTYEDLILNYPNSYMVETARKKLQILQNVKTGLQ"
     gene            590..>1336
                     /gene="rsmA"
                     /locus_tag="PK976_07140"
     CDS             590..>1336
                     /gene="rsmA"
                     /locus_tag="PK976_07140"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005468060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HRC67697.1"
                     /translation="MSKQDYNSQKVSPKKFLGQHFLHDKNIARKIVESLSLQTHNILE
                     IGPGMGMLTQYLLQKPNINLKLVEIDNESVVYLKERFPQIAENIIFSDFLQLDIHSIF
                     NEPFAIIGNFPYNISSQILFKVYEHVEMITELSGMFQAEVARRIISGPGNKDYGILSV
                     LIQTFYDGKYLFTVNENVFTPPPSVKSGVIHLKRNPGKHLPCPLEDFTLLVKTAFNQR
                     RKVLRNALSRFQFNFDAHVEVLLQKRAEQLN"
BASE COUNT          424 a          269 c          267 g          376 t
ORIGIN      
        1 gctttaatct atggacgttt ggaaagagat tttcccaatc atccccttgg agaagaagcc
       61 aagttagaat atgccaaact aatgtatcag gctggggagc ataaatgggc tttgcttcat
      121 cttgaaattc taaggggagc tattcataaa cctgtggcca acgatgccca ggaattgtat
      181 ctattcatac aggaggcttt gaacgaggat accaccggtc ggatattgca ttactttggc
      241 agcagtgaat tgcttagtat tagaaaacaa tacgccatgg cacttcgaac gctcgatagc
      301 gtttcgatgt tctttggaaa tagctggggc caggattatg cattgttgag aaaagcaagg
      361 atttataagg aaatgaggga atacaatata gccgattcca tttattctac tcttgtgaga
      421 atttttcctc gaagcctggt tgccgacgat gctctattag agcttgcttc cttaagagaa
      481 ataacccatc aacccgaaaa ggcctataaa acttatgagg acttgatttt aaactatcca
      541 aacagttata tggtcgaaac tgctcgcaaa aaacttcaga ttctgcaaaa tgtcaaaaca
      601 ggattacaat agccaaaagg taagtccaaa aaagtttctc ggacagcatt tcttacatga
      661 taagaacatt gcccggaaga ttgtagagag tttgagcctc caaacgcata acattcttga
      721 gatcggcccg ggcatgggta tgttgaccca atatttattg caaaagccaa atataaacct
      781 caaactcgtc gaaatcgaca atgaatcggt agtttatctc aaagaaagat ttccccaaat
      841 agccgaaaac atcatctttt ccgattttct tcaactcgat attcatagta tatttaatga
      901 acctttcgcc atcataggaa attttcccta taatatatct tcacaaattc ttttcaaagt
      961 ttatgaacat gtagaaatga tcaccgagct tagcggaatg tttcaggccg aagtggctag
     1021 gcgcatcatc agcggtccgg gaaacaagga ttacgggatt ttaagtgttt taatacaaac
     1081 attttacgat gggaaatatc tttttacggt aaatgaaaat gtatttactc ctccaccgtc
     1141 agtgaaatcg ggtgtgattc atttgaaacg caatcctggc aaacatctcc catgtccgct
     1201 cgaggatttc accctactgg tcaaaacggc cttcaaccaa agaagaaaag ttctgcgcaa
     1261 tgcactttcg agattccagt ttaatttcga tgcgcatgtc gaagtattgt tgcaaaaacg
     1321 tgccgagcaa ctcaat
//
LOCUS       DAORPD010000598         1334 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_20266_length_1334_cov_2.000000, whole genome shotgun sequence.
ACCESSION   DAORPD010000598 DAORPD010000000
VERSION     DAORPD010000598.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20266_length_1334_cov_2.000000"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            16..1278
                     /locus_tag="PK976_07145"
     CDS             16..1278
                     /locus_tag="PK976_07145"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HRC67698.1"
                     /translation="MTESFVHYLWKNNLLKPELQTTSGQPITIIQPGIHNEDAGPDFI
                     QAIIKIEDEIWAGNVEIHLHTSDWYKHGHEKDKAYQNIILHAVFENDLSGQEPKEFPT
                     LELKPFTDPCLWNRYQTFLMSKDWIPCARNFHEAFPLTTHSMLEISLVMRLERKIEEI
                     SKIVTLNKNNLSEAFYIILTRNFGLKANIQPFELLARSLPLNLLAKHKGNLFQIEALM
                     FGQAGMLAEASDDYQRKLKEEFHFLKNKYYLTPIPVYLWKFLRMRPASFPTIRIAQLA
                     ALINTSSHLLSKLSECNSLIEMEELLACRASNYWDTHYRFGEISAVRPKNAGKDFIHS
                     IIINSVIPFIHIQAVYTGKSGHQKHIENLLSDLPPENNQILRGWETLGYKAKDAFESQ
                     ALIELKTHYCNLKRCLDCRIGHSLLTQK"
BASE COUNT          424 a          296 c          237 g          377 t
ORIGIN      
        1 taatataaaa atggaatgac cgaatccttt gtgcattatt tatggaaaaa caacctgttg
       61 aaaccagaac tgcaaaccac atcgggtcaa cctattacca ttattcaacc aggaatacac
      121 aatgaagatg cagggcccga ttttattcaa gccataataa aaatcgaaga tgaaatatgg
      181 gccggaaacg tcgaaatcca tttgcataca tctgattggt acaagcatgg gcacgagaag
      241 gacaaggcct atcaaaacat cattctgcat gctgttttcg aaaatgatct cagtgggcaa
      301 gaacccaaag aatttcccac tctcgagctg aaacccttta ctgatccatg cttatggaat
      361 cgttatcaaa cttttttaat gtcaaaagac tggatcccct gtgcaagaaa ttttcacgaa
      421 gcatttccac ttaccacaca ttccatgctc gaaatttccc tcgttatgcg cctcgaacgg
      481 aagatcgaag agatttcaaa aatcgtgacc ctgaataaaa acaatctttc agaagccttt
      541 tatatcattt tgacacgtaa ctttggttta aaagccaata ttcaaccatt tgaactttta
      601 gccaggtcat tgccattgaa tttattagcc aaacacaaag gcaatctttt tcagatcgaa
      661 gccctcatgt tcggtcaggc aggcatgctt gccgaggctt ctgacgatta ccaaaggaaa
      721 ttaaaagaag aatttcattt cctgaagaac aaatattatc tcactcctat tcccgtctat
      781 ttgtggaaat ttttaagaat gaggcctgca agttttccta ccattcggat tgcacaattg
      841 gccgcgctca tcaatacttc atcacatctt ttgtcgaaac tctcagaatg caattctctc
      901 atcgaaatgg aagaactttt ggcttgccgg gcttcgaatt attgggatac acattatcgt
      961 tttggagaaa ttagtgccgt cagaccaaaa aatgccggaa aggattttat ccattctatc
     1021 attatcaact ctgtcattcc tttcattcat attcaggccg tttatacggg caaaagcggc
     1081 catcagaaac atatcgaaaa tcttttatcg gacttgcctc ccgaaaacaa tcaaatttta
     1141 agaggctggg aaacgcttgg gtataaagcg aaagacgcat tcgaaagtca ggctcttatc
     1201 gaactcaaaa cgcattattg caacctgaaa cgttgcctcg attgcagaat cggacattct
     1261 ttgcttaccc aaaaataacg atgacatcgg ataaatcagg ctaatttctt cccatacggt
     1321 tttcctgtcg cgtg
//
LOCUS       DAORPD010000599         1332 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_20313_length_1332_cov_2.182459, whole genome shotgun sequence.
ACCESSION   DAORPD010000599 DAORPD010000000
VERSION     DAORPD010000599.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20313_length_1332_cov_2.182459"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      complement(326..426)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(993..>1332)
                     /locus_tag="PK976_07150"
     CDS             complement(993..>1332)
                     /locus_tag="PK976_07150"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRC67699.1"
                     /translation="AFASSQIRVMAGALGFPILSFALHQSNSLKKTFHDKVAMKSILI
                     GSIFGPFLGVSFSLIAIQHTKTGIASTLMALTPVMILLPSAILFHQKIVWKEIVGAII
                     SVSGASLFFL"
BASE COUNT          450 a          220 c          257 g          405 t
ORIGIN      
        1 tttaatcatt cacttatata taatgaaaaa atgcttttat ttaaggcatt atcgtattaa
       61 aaatgacttt cttaaagaga aaaaaacggc aataacgaat tgattttaac gaggtttcat
      121 cagcttttta taattgtacc aaaaataata atgtattttg gatgctgtct ttaaaatttt
      181 attgatttaa agtcgagatc tgttttctca cgaaggtgct atctaatata aagaaggatg
      241 tggagatgtg cataaaaaaa ctccctactg ggtaaatgcc caacagggag ttttgagtcg
      301 tatagaatta ctcattacct tattgaactt atctttccca gttttagggc aggagttggc
      361 accttaccac gagggcaggt tgccaaggtt tcacagggcc attccctcca cctttctcga
      421 taagccactt catgcaaaga acagactgca aatataaaga actttttcga gtataataat
      481 accagagtac tgaagacctt tcgaaaaaat gtgtcagctt tctttggtcg acattttatt
      541 atccagcaga cgcattcttt ttagaataac cctgaaaata tgcctttgtt tgcatgttgg
      601 cataaagaat gttctcattt cttgtcttgc ggtgcttcaa gaaagatatg agaaaagctt
      661 aggcgagttt ggaaccaagt cccacacagc aatatatttt tgatttttta gataagacca
      721 actacatcga atatccattc atcgaaagag ccattagact gttgcctgaa attttcgatt
      781 aaggacaaca atacacttaa gataaattga agataaagct gccatcgatc acaaaatctt
      841 tcttattttg atttcaaaga atacatagat gaatagcaaa cgaatgtatg aatgcttctt
      901 aatttgagtg atattttaaa aatctttgct gacttgatga atgatttaat atttaggaat
      961 aaagacaaaa ggaaaataga atcagttgct ttttatagga agaacaatga agcaccgctc
     1021 acgctgataa tagctccgac aatttctttc cagacgattt tttggtgaaa caggatggcc
     1081 gaagggagaa ggatcatgac tggcgtaagg gccatgaggg tagatgcaat tccggttttg
     1141 gtatgttgga tagcgatgag agaaaatgat acgcccaaga aaggtccaaa aatagagcct
     1201 atcaagatgc ttttcatggc taccttatcg tgaaaggttt ttttcaagga atttgactga
     1261 tgcaatgcaa acgaaagaat gggaaaaccc aatgcaccgg ccataactct tatttgcgaa
     1321 gaggcgaaag ca
//
LOCUS       DAORPD010000600         1332 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_20317_length_1332_cov_2.070478, whole genome shotgun sequence.
ACCESSION   DAORPD010000600 DAORPD010000000
VERSION     DAORPD010000600.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20317_length_1332_cov_2.070478"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(148..843)
                     /locus_tag="PK976_07155"
     CDS             complement(148..843)
                     /locus_tag="PK976_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HRC67700.1"
                     /translation="MKFIIIGLGNFGTSLAIKLTDMGHEVIGVDKQMEKVDEIKDKIT
                     MAFCLDCKYQEAIASLPLKNTDVAIVGIGEDEGANLLTTALLKKAGVARLISRAVSPI
                     HATILEAMEISEIVRPEEETAERWAKKLSTSGIVDSFELTDKYSIIEVKAPKSFWGKT
                     IEQIGFSKNYNVIVLTIMKNVKEKNILGIFRRVTKPQIQGIAKADSVILEGDILVLYG
                     HNDDIHNLLKISR"
     gene            complement(857..>1332)
                     /locus_tag="PK976_07160"
     CDS             complement(857..>1332)
                     /locus_tag="PK976_07160"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HRC67701.1"
                     /translation="ISTLLITILLMWIGASPASTGGGIKTSSIAVAWLNILSIIRGKQ
                     RVEAFKREISPVSIHRAFAVIFLSIFVIFLSIFFVSIFETDKGILNIAVECISAFGTV
                     GLSTGITPHLCGASKIVLILTMFVGRINMLTILIAFFKKSSTWVGRYPSESILIN"
BASE COUNT          406 a          267 c          241 g          418 t
ORIGIN      
        1 atatttgact ccatcgaaaa cttcgaatgc ttcatcgtca tttttttgtt cgattttttc
       61 ctcttacaaa acttctggga agtttgacat cggaaataaa gaaaggatta aagggcgatg
      121 cacggagggg atcgcctttt gatttgttta tcgactgatt tttagcaggt tgtgaatatc
      181 atcattatgt ccataaagaa caagaatatc gccttccaaa attaccgaat cggctttagc
      241 aatcccttga atctgaggct tggtgacacg tctgaaaata cccagaatgt ttttttcttt
      301 cacatttttc ataatggtaa ggacaataac gttataattc ttgctaaaac caatttgttc
      361 tatcgttttt ccccagaagc ttttaggagc ttttacttcg atgatactgt acttatctgt
      421 taattcgaat gagtcgacta taccgctggt ggaaagtttt ttagcccagc gttcggcagt
      481 ttcttcttct ggtctgacaa tttctgatat ttccattgcc tccagaatag ttgcatgtat
      541 aggagataca gcccggctga tcaaacgtgc aactcctgct ttttttaata gagcggttgt
      601 aagcaaattg gccccttcat cttctccaat gccaacaatc gccacatctg tatttttaag
      661 aggtaggctg gctatggctt cctggtattt acaatccaaa caaaaagcca tggtaatttt
      721 atccttgatt tcgtctactt tttccatttg cttatccaca ccaattactt catgacccat
      781 atcagtaagt tttatagcca gagaagttcc aaaatttccc aatccaatga tgataaattt
      841 cataatttaa acgcatttag ttgattaaga tactttcaga agggtaacgt ccaacccatg
      901 tagatgattt tttgaagaat gcgatcaaaa tagtaagcat atttattcta ccaacaaaca
      961 tggttaaaat taatacaatt ttgctagctc cgcacaaatg aggcgtaatt ccggtactga
     1021 ggccaacagt gccaaaggcc gatatgcatt cgacggcaat attcaggatg cctttatcgg
     1081 tctcgaatat tgatacaaag aatatggata ggaaaataac aaaaatggat aagaaaatga
     1141 cggcgaaggc ccgatggatc gaaacaggag aaatttcacg tttaaaagcc tccactcgtt
     1201 gtttccctcg aataatactt aaaatattca accatgccac ggcaatagaa ctcgttttaa
     1261 ttccaccgcc tgtcgaagct ggcgaagctc ctatccacat caacaaaatg gtaatgagga
     1321 gagtagaaat at
//
LOCUS       DAORPD010000601         1330 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_20368_length_1330_cov_2.214118, whole genome shotgun sequence.
ACCESSION   DAORPD010000601 DAORPD010000000
VERSION     DAORPD010000601.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20368_length_1330_cov_2.214118"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /gene="tpx"
                     /locus_tag="PK976_07165"
     CDS             complement(<1..492)
                     /gene="tpx"
                     /locus_tag="PK976_07165"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HRC67702.1"
                     /translation="MKKNKGITTFMGKPVTLLGDMVQPGEIAPNFTALAADSKPVNLS
                     DFKGKVIIISSVPSVDTGVCAAQTRRFNTEAASLGEVVILTISCDLPFALGRFCAAEG
                     IDKVITLSDHKDTDFGLKYGFLIEELRLLNRGIVVIDREGIIRYIEYVKENTHHPDYD
                     AAIA"
     gene            747..>1330
                     /locus_tag="PK976_07170"
     CDS             747..>1330
                     /locus_tag="PK976_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRC67703.1"
                     /translation="MATQQKGKINVQTENIFPIIKKFLYSEHEIFLRELISNAVDATT
                     KLKALASIGEFKGELGDLRIEVKVDKKKKTLTVSDKGIGMTAEEIDKYINQIAFSSAE
                     EFISRFKDKNDTEVSQLIGHFGLGFYSAFMVADKVRIESLSWTEGAEAVVWECDGSPE
                     YAMKPSNKKTRGTDVTLYINKDNEEFLDEYRVLEL"
BASE COUNT          440 a          261 c          282 g          347 t
ORIGIN      
        1 agctattgcc gcatcatagt caggatgatg ggtattttcc ttcacgtact ctatataacg
       61 gatgatgccc tccctatcga tcacaacgat tccacggttg agtaaacgca gctcttctat
      121 caaaaaacca tatttcaagc cgaaatcggt atctttatga tcggatagag tgatgacctt
      181 atcgatgcct tctgcggcac aaaatcttcc caaagcgaaa ggaaggtcgc aggagatggt
      241 aaggataaca acctcgccca aagaagcagc ttcggtattg aaacgtcggg tctgggcagc
      301 acatacacct gtatctaccg agggtacact gctgatgata atcactttac ccttgaaatc
      361 gctcagattt acaggttttg aatcggcggc taaagcagtg aaattaggag caatctcacc
      421 tggctgcacc atatcgccca gtagtgtcac cggcttgccc atgaaagtag tgatcccttt
      481 attctttttc atgatgggtc aaattaaatt gatattatta agatatatga aataaacaaa
      541 aatttccaat gaaagttcag gtaaaaaaac aaaatatagg cacattcctt taatccacga
      601 aatcgaaatc ctcgtcattc ttaaaatatc gaataagaac ctaattcgtc tgatgacatc
      661 caacaaaaca accttgccat tttgtcagag tatatcgggc tggcacaggc tttgccaatg
      721 gaatagcgtt tcaaaaaatg tcaattatgg ctacacagca aaagggtaaa atcaacgtac
      781 aaaccgagaa tatttttccc atcattaaga agtttctgta ttcggaacat gagatttttt
      841 tacgtgaact tatctcgaat gcagtagatg cgaccacgaa attgaaggca ctggcttcga
      901 taggcgaatt taaaggggag ttgggcgatc ttcgtataga agtaaaagta gataaaaaga
      961 aaaaaactct caccgtatcc gataaaggca taggcatgac agccgaagaa atagacaagt
     1021 atattaatca aattgcattt tcgagtgccg aagaatttat cagccgcttc aaggataaga
     1081 acgataccga agtcagtcag ttaatcgggc attttggtct gggattttat tcggcattta
     1141 tggtagccga taaggtaagg atagaaagtt tatcgtggac agaaggagct gaagctgtgg
     1201 tctgggaatg cgacggttcg cccgaatatg cgatgaaacc ctcaaataaa aaaactcgtg
     1261 ggaccgatgt aaccctatat ataaataaag ataacgaaga atttctcgac gagtacaggg
     1321 ttctggaact
//
LOCUS       DAORPD010000602         1328 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_20403_length_1328_cov_2.058130, whole genome shotgun sequence.
ACCESSION   DAORPD010000602 DAORPD010000000
VERSION     DAORPD010000602.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20403_length_1328_cov_2.058130"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            345..>1328
                     /locus_tag="PK976_07175"
     CDS             345..>1328
                     /locus_tag="PK976_07175"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HRC67704.1"
                     /translation="MKNFLFLSIPMIVLPIILSISSCSKDDETPKQPKPIVLPEKSTE
                     VISCTNDFGIELFSLVAQGNDDNLMLSPLSASIALTMALNGSKGNTWQQISDLLGFHD
                     LTQEEVNQIYHQLNTQLLTIDPEVELSLANAVWYRNSFTVKQEFIRTMQEVFDAQTQS
                     LDFNNPEALKTINQWASDCTKGKITKVLTEINPYEVMFLMNALYFKGTWTYSFDKKLT
                     TDAFFYPEGQVPLKVSMMNGEIPLKIYGTNDFTAVEMPYNQQNFVMDILLPTGKLSEF
                     LSNFTSDTYNSLMTSFESIEPSKIHLSMPKFAFSYEKKLNEQLKTMGMTDAF"
BASE COUNT          448 a          248 c          225 g          407 t
ORIGIN      
        1 agcaaaaata agtaataaga taaagatttg agaaatgata aggcaattca tcaaaaaaat
       61 tagcctatat cattcttacg aagtttgtag aatacttagc gtcgcgaaaa ctattgtttg
      121 tagtaggaca taatttgttt attaaggcaa agtattgaag cgtcaaaagc aaaagggtga
      181 tttagaaaag cccttttggg cgattcgatg gctaagcaat ccaaattttc caatatttac
      241 ccgaaataat ggttctgaat taaaataact catttaatcc tatttcaaat atttatggtt
      301 tgattattgc tattttttct tatacatttc aaacatcatc aagtatgaaa aacttcttat
      361 tcctctccat tcccatgatt gttttgccaa taattcttag tataagttca tgcagtaagg
      421 atgacgaaac tccaaaacaa cccaaaccaa tcgttttacc tgaaaaatca accgaagtca
      481 tttcttgcac caacgatttc ggcatagaac tattcagttt ggtagcacag ggcaatgatg
      541 acaatttgat gttatctccc ttgagtgcaa gcatagccct tactatggca ctgaatggct
      601 ctaaaggaaa cacctggcaa caaatcagcg acctgttggg ttttcatgat ttgacgcagg
      661 aagaagtaaa tcaaatatat caccaactca acacccaatt attgactatc gaccctgagg
      721 ttgagctttc gttggctaat gcagtctggt atagaaatag cttcactgta aaacaagaat
      781 ttattcgaac catgcaagag gtttttgatg cacaaactca atcgctcgat tttaataatc
      841 ctgaagcact aaaaaccatc aatcaatggg catccgactg cacgaaagga aaaattacca
      901 aagtgcttac cgagatcaat ccctacgagg tgatgttttt aatgaatgcc ctctatttta
      961 agggtacttg gacttattct tttgataaaa aactcactac tgatgcattc ttttatcctg
     1021 aaggacaagt tcccctgaaa gtttctatga tgaatggaga gattccttta aaaatttatg
     1081 gcacaaacga tttcacagcg gtggaaatgc cttataatca gcagaatttt gtgatggaca
     1141 tacttttacc aacaggaaaa ttatcggaat ttttaagcaa tttcacttct gatacatata
     1201 attcactgat gacctctttt gaatctatag agccttcgaa aattcatctt tcgatgccta
     1261 aatttgcttt cagttacgag aagaaactca atgagcagct caaaacgatg ggtatgaccg
     1321 atgccttc
//
LOCUS       DAORPD010000603         1328 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_20407_length_1328_cov_1.794973, whole genome shotgun sequence.
ACCESSION   DAORPD010000603 DAORPD010000000
VERSION     DAORPD010000603.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20407_length_1328_cov_1.794973"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1328)
                     /locus_tag="PK976_07180"
     CDS             complement(<1..>1328)
                     /locus_tag="PK976_07180"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016744 - transketolase or transaldolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HRC67705.1"
                     /translation="EKLQKDAVNTIRFLSADAVQKANSGHAGLPMGAAAIGYTLYKRH
                     LRFNPKNPNWPNRDRFILSAGHGSMLLYSLLYLTGYDLPFEELKQFRRWGSLTPGHPE
                     YGITKGVETTTGPLGQGLATAVGMAIAEAHLAAVYNRLGFQIIDHYTYVLASDGDLME
                     GVTSEACSLAGHLQLGKLIVFYDDNRVTIDGGTEISFTEDRAKRFEAYQWQVLHVEDG
                     NNVEELDKAIKLAKVDPRPSIIICRTHLGYGYPTWQDKSIAHSGIPSKEELDGAKRAL
                     GFPTEPAFYVSDEVLAHFREAVQTGAQLETAWKSLFYRYAEAYPQLASELKRILNGEL
                     PAGWEESLPFFAADAKGMPTRSASNKVINALAKLMPELMGGSADLTTSNSTMIENTLD
                     FQPGQYEGRYIYFGVREHAMGAILNGLVVHGGVVPFAGTFLVFSDYLRPA"
BASE COUNT          351 a          331 c          291 g          355 t
ORIGIN      
        1 cgctggcctc aaataatccg aaaagaccaa gaaggttccc gcaaaaggaa ctactccacc
       61 atgcaccacc aagccgttca agatggcacc catcgcatgt tcacgcaccc caaaataaat
      121 atatctgcct tcatattggc caggctgaaa atcaagggta ttctcgatca tggtagagtt
      181 cgaggtagtt aaatcagcag agcctcccat gagttccggc attagcttcg ccaaggcatt
      241 gatcacctta ttggaagcac ttcgtgttgg cattcctttg gcatctgctg caaagaaagg
      301 caggctttct tcccagcctg ctggcaattc tccattgagg atgcgcttta attctgatgc
      361 gagttgaggg tatgcttcag cgtaacggta aaatagggat ttccaagcgg tttcgagttg
      421 agcgccagtt tggactgctt ccctaaaatg cgctaagact tcatcgctga cgtagaatgc
      481 aggctccgtc ggaaacccta aagccctttt tgcgccatct aattcttctt tgctcggtat
      541 gccgctatgg gcaatggatt tatcttgcca ggtaggataa ccgtatccaa gatgcgtccg
      601 gcaaatgata atagaggggc gaggatctac tttcgctaat ttgatggctt tgtctaactc
      661 ctccacatta ttgccatctt ccacatgcaa aacctgccac tgataggctt caaatctctt
      721 ggcacgatcc tccgtgaagc taatctctgt acccccatcg atcgtaaccc ggttatcatc
      781 ataaaatact atcagttttc ctaattgcaa atggcctgca agggagcatg cttcagaggt
      841 tacaccttcc attagatcac catccgaagc taaaacgtat gtatagtggt caataatttg
      901 aaaacctaag cggttataaa cagccgctaa atgcgcttcg gcaatagcca tgccgacggc
      961 agtagctaag ccttgcccta aaggaccagt agttgtctct acccctttgg ttattccata
     1021 ttccggatgg ccaggagtca aacttcccca acggcggaac tgctttagct cttcgaaggg
     1081 taaatcatag ccggttaaat aaagaaggct atagagtagc atcgacccat gcccggcaga
     1141 taatataaac cggtcgcgat taggccaatt tgggtttttg gggttgaacc taagatgcct
     1201 tttatagagg gtataaccaa tagctgcggc tcccatgggc aaacctgcat gcccagaatt
     1261 cgctttttgc acggcatctg cggataaaaa tcgaattgta ttgaccgcat ctttctgtaa
     1321 tttttcct
//
LOCUS       DAORPD010000604         1326 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_20468_length_1326_cov_1.912667, whole genome shotgun sequence.
ACCESSION   DAORPD010000604 DAORPD010000000
VERSION     DAORPD010000604.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20468_length_1326_cov_1.912667"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="PK976_07185"
     CDS             complement(<1..614)
                     /locus_tag="PK976_07185"
                     /inference="COORDINATES: protein motif:HMM:NF014923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HRC67706.1"
                     /translation="MLTFAKKFIPLIPPNTHNFVAKTLAGLEEVLEQELKELGATHTQ
                     ILTRAVSFEGTRETVYRTNYHCRTAIKILVPLIQFKASNNEELYEGVRSFQWDQIMNF
                     RDTFMVETVLVHSAFQHSHFISLKTKDAIADWFTNKYKRRPNVDRENPDIRLSLYIHR
                     DECTLYLDSSGDPLFKRGYRMAQGPAPMSEVLAAGLIKLSGWNG"
BASE COUNT          402 a          238 c          329 g          357 t
ORIGIN      
        1 tccccattcc accccgatag cttaatcagg ccggcggcca gaacttcgct catgggtgca
       61 ggtccttgag ccatgcggta acctcgtttg aacagggggt cgcccgaact atcgagatat
      121 agcgtgcact cgtcgcgatg aatatataaa ctcagacgaa tgtcgggatt ttcacgatct
      181 acattcggtc ttcttttgta tttgttggta aaccaatcgg ctatggcatc tttggttttc
      241 agcgagatga agtgcgagtg ctggaaggct gaatgaacca ggacggtttc caccataaaa
      301 gtatctctga aattcatgat ctggtcccat tggaaagaac gcacgccttc gtataattct
      361 tcgttgttgc ttgctttgaa ttgaatgaga ggaacaagga tcttaatagc cgtccggcag
      421 tggtaattcg tgcgatagac ggtttcacgc gtgccttcga aagaaactgc tcgggtaaga
      481 atttgagtat gggtggctcc aagctctttt agttcctgtt ccagaacctc ttcgaggccg
      541 gcaagagttt tggctacaaa attatgggtg ttagggggta tcaaaggaat gaattttttt
      601 gcaaaggtaa gcacttatgc ctgatagaat ttttttcatt tctctcatag cggtttcatg
      661 catcgatatt gcttcactca ttttgtggat ggtttttttc aggatcaaaa tcaaaacaaa
      721 aatggaaacg agtataaaaa tgaggataaa gatttgatgg gagttctata aggatggtga
      781 ttcgatgaaa acagcgaggc tacgaggttg gtttacagcc agaaaaaata gaaaaattcg
      841 tgagaatatg cctgacattg taccggcaaa gtaatttgca agatgaatct ccatgcataa
      901 caaagcaaag gtggtcatgg caaaggcgag gcaggagaga ggcaacagaa gaaaaattga
      961 agaaattgtt gattggaaag atagtcaaag aatctataaa gtgctggatc gataaaagta
     1021 gtggaaccca ttgccagttt attggttcct ttgccaaaag cgatatctat ccaggcagcc
     1081 tatataccca catcatggca atccagtcca tccaaaatag caacccgaaa ttacggcgat
     1141 aaaagttctg agagcaagag cgaactacat agtaaatgaa gagaagagat agcatagggt
     1201 tgcattgcag atgttagagg tgatggaagg cgtgctactg tttaaggcca agaaaaatga
     1261 aggagaaatg atgtcgggta atacgacggg aatgcctatt gaaccaaact ctgttttttg
     1321 gttttt
//
LOCUS       DAORPD010000605         1321 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_20587_length_1321_cov_2.227488, whole genome shotgun sequence.
ACCESSION   DAORPD010000605 DAORPD010000000
VERSION     DAORPD010000605.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20587_length_1321_cov_2.227488"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1314)
                     /gene="atpA"
                     /locus_tag="PK976_07190"
     CDS             complement(<1..1314)
                     /gene="atpA"
                     /locus_tag="PK976_07190"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005787491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HRC67707.1"
                     /translation="MKPAEVSAILRQQLAGHRTTAELNEVGSVLQVGDGIARIYGLKN
                     AQSGELVEFENTGVKAIVLNLEEDNVGVVLLGESNLVKEGDIVKRTGEVTSVEVGEGM
                     LGRVINTLGESIDGKGDIPGPLYRMPLERKAPGVIYRRPVNEPLQTGIKAIDAMIPIG
                     RGQRELIIGDRQTGKSSIALDTIINQKEFYEKGEPVYCIYVAVGQKGSSVANFVKTLE
                     DFGAMPYTIVVMATASDPAAMQFYAPFTGAAIGEYFRDTGRPALIIYDDLSKQAVAYR
                     EVSLLLRRPPGREAYPGDIFYLHSRLLERAAKIIDSDQIAAQMNDLPESIRDKVKGGG
                     SLTALPIIETQAGDVAAYIPTNVISITDGQIFLETNLFNSGIRPAINVGISVSRVGGN
                     AQIKGMKKIAGTLKIDQAQYRELEAFSKFGSDLDPATIAILEKGKR"
BASE COUNT          312 a          316 c          286 g          407 t
ORIGIN      
        1 tcgttttcct ttttccagta tggcaatggt tgctggatcg aggtcagaac caaatttgga
       61 gaaagcctct agttcgcggt attgtgcctg gtcgattttc aaggtgcctg caattttttt
      121 catgcctttg atctgtgcat ttccacctac tctcgaaacg gagattccaa cgttaattgc
      181 cggtcggatt cctgaattga aaagattggt ttcgaggaaa atctgtccat cggtaatgga
      241 aatcacattg gtagggatat aagctgctac gtcaccggct tgagtttcga ttataggaag
      301 ggctgtcagt gagcctcctc cttttacttt atcgcggata gattcgggaa ggtcgttcat
      361 ttgggctgca atttggtcgc tatcaatgat ttttgctgca cgttcgagca aacgagaatg
      421 caggtagaag atatctcctg gataggcttc acgccctgga ggtctgcgca ataaaagtga
      481 aacttcacga taggctactg cctgtttgga gaggtcatca taaataataa gagctggacg
      541 tcctgtgtct ctaaaatatt ctcctatggc cgctcctgtg aaaggagcat aaaattgcat
      601 ggctgccgga tcactggcag tagccattac tacaatcgta taaggcattg ctccgaaatc
      661 ttcgagtgtt ttcacaaaat tggccactga cgagcctttt tgccccactg ctacataaat
      721 acagtaaacc ggttcgcctt tctcataaaa ttctttttga ttgatgatag tgtcaagggc
      781 aatagatgat tttcctgtct ggcggtcgcc aataataagt tctctttgcc ctctcccaat
      841 agggatcatt gcatcgattg ctttaatccc ggtttgaagg ggttcattta caggccgacg
      901 gtaaattact ccgggagctt ttctttcgag aggcattcta tagagaggcc ctggtatatc
      961 tcctttccca tcgatggatt cgccaagtgt gttgatcacc cgtcccagca tgccttctcc
     1021 tacttctacc gaagtaactt ctccagtccg ttttacaatg tcgccttcct ttaccaagtt
     1081 cgattccccc aatagcacaa cccctacatt atcttcttcg agattaagaa caatggcttt
     1141 tactccagtg ttctcgaatt ctaccagttc gcctgattgt gcattcttca atccataaat
     1201 acgggctatg ccatcgccta cctgcaatac acttcccact tcgttgagtt cggcagtggt
     1261 cctgtggcca gctaactgct ggcgtaaaat cgcagaaact tccgccggtt ttatttctac
     1321 c
//
LOCUS       DAORPD010000606         1320 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_20609_length_1320_cov_2.569960, whole genome shotgun sequence.
ACCESSION   DAORPD010000606 DAORPD010000000
VERSION     DAORPD010000606.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20609_length_1320_cov_2.569960"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="PK976_07195"
     CDS             complement(<1..394)
                     /locus_tag="PK976_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67708.1"
                     /translation="MNKTFPLYLLSFLILLAACKTGRQTTQQPQQTAYSQTPAPLMVY
                     KTRYNYVNLVPIVLSQNKDSILHYPHPQDLKYGNSFRYPVLLEQGYLLDKKGIQPGTA
                     FLRLTYEEYAAFKDLPSQHQLLQMIADTN"
     gene            complement(387..>1320)
                     /locus_tag="PK976_07200"
     CDS             complement(387..>1320)
                     /locus_tag="PK976_07200"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRC67709.1"
                     /translation="VSALPERILVPVANPQSLQSLLELAIVLKHPGSDEPIYPISIVE
                     DNDQVQQNILKSRKNLDNIIQHISLSEVKIQPLVRVDVNIVSGIIRGIKETMASMIVM
                     GWGGSLKTSERIFGSILQKIISETEQTTLVSRIKQPVFIFKNINVLCPPNAEYEVGFI
                     YWVSKILIMAYRIQATLEFKTSKDLLPVFSTIARRWKINPSLKNTIYSDWETGFESSS
                     YSKTDDLFVIVAARKDTISFLPVFDSLPQRLNHLPENLNFILLFPEQVEKMKGTGVIP
                     VSEIHISPIMSQMENLKKISKKVMKSVQKKSSHE"
BASE COUNT          367 a          228 c          298 g          427 t
ORIGIN      
        1 gattggtgtc tgcaatcatt tgaagcaact gatgttgcga gggcaaatct ttaaaagcgg
       61 catattcttc ataagtaagc cttaagaatg ccgtgccggg ttgaattccc tttttgtcga
      121 gcaggtagcc ttgttccagt aaaacaggat agcggaaaga atttccatat tttaggtctt
      181 gggggtgagg gtaatgtaga atggaatctt tattttggct tagcacaatg ggaactaaat
      241 tcacgtagtt ataacgtgtt ttatatacca tgagaggagc aggcgtttgc gaataagctg
      301 tttgttgagg ttgctgagtg gtttgccggc ctgttttaca agctgccaac agaatgagaa
      361 acgaaagaag ataaagagga aaggttttat tcatgacttg attttttttg gacagatttc
      421 attacttttt tactgatttt tttgaggttt tccatttgcg acatgatggg agaaatatga
      481 atttcgctta ctgggattac tccagtgcct ttcatttttt cgacttgttc ggggaacaga
      541 agaatgaagt tgaggttttc gggtaagtga ttcaatcgtt gaggcaaact atcgaaaacg
      601 ggtaaaaacg atatggtatc tttccgggct gcgactataa caaagagatc gtcggttttt
      661 gaatagctac tcgactcaaa acctgtttcc cagtcggaat agatggtatt tttaagagat
      721 gggttgattt tccatcttct ggctatggtt gaaaaaaccg gcaacaggtc tttcgaagtc
      781 ttgaactcaa gggttgcttg aattctgtaa gccattatca aaattttcga gacccagtaa
      841 atgaatccca cttcatattc tgcattaggt gggcataata cgttgatatt cttgaaaata
      901 aaaacaggct gcttgattct gcttactaaa gtagtttgct cagtttcgga aatgattttc
      961 tgaagaatac ttccaaagat acgttcactt gttttcaagc ttcctcccca gcccataacg
     1021 atcatactgg ccatggtttc cttgattcct ctaataatcc cactaacaat atttacatct
     1081 accctgacca gtggttgaat cttgacttca ctcagtgaaa tatgctggat gatattatcc
     1141 aagttttttc ggcttttaag gatgttttgc tgtacttggt cattatcttc aacaatggat
     1201 ataggataga tgggttcatc cgatccggga tgtttcaaaa cgattgccaa ttctaacaaa
     1261 gattgaagcg attgggggtt ggctacggga acaagaatcc tctcgggcaa ggcagagact
//
LOCUS       DAORPD010000607         1314 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_20766_length_1314_cov_2.326450, whole genome shotgun sequence.
ACCESSION   DAORPD010000607 DAORPD010000000
VERSION     DAORPD010000607.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20766_length_1314_cov_2.326450"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..525
                     /locus_tag="PK976_07205"
     CDS             145..525
                     /locus_tag="PK976_07205"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HRC67710.1"
                     /translation="MAKNTIIESPIVNIIGSMTSIEGDVSCGGDIRIEGQLKGKLECK
                     GKVVIGESGVVEGEIYCRNGDFAGKIKGSVNTYELLTLKSTVVLQGDITCSKLAIEPG
                     ARFSGKCTMELPENVQAAGQNEKE"
     gene            512..766
                     /locus_tag="PK976_07210"
     CDS             512..766
                     /locus_tag="PK976_07210"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HRC67711.1"
                     /translation="MKKNDIKPSLFNYAKYSSLAFQMLVVILIGVLGGIKLDQYLQLK
                     FPVFTILFSIAAVFIATYLGMKDFIKRPPKPSQKIDHNDE"
     gene            756..1145
                     /locus_tag="PK976_07215"
     CDS             756..1145
                     /locus_tag="PK976_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67712.1"
                     /translation="MTNEFKHYLRKLIILSVFLAILGTILKFVLPKGIITPAMPFILI
                     FFMAHTLVYNNMAVNMIHQNPKKFVNFYMLSTVGRLILFVLLLVLYAFLVPKDFKAFT
                     VCFFIFYLIYTFFELSTLLPQLRKKKP"
BASE COUNT          400 a          250 c          242 g          422 t
ORIGIN      
        1 atttccacga aaaactatca aaccttttat caggatagag atatttcaaa atataagtct
       61 ttctttaata aggaatatct gaagaaaaat taaggatttc gataaaatca gtgattttta
      121 gtgtaaattt gaaaaaaata tactatggct aaaaatacta ttatagagag ccccattgtg
      181 aatatcatcg gttcgatgac atctatcgaa ggcgatgtga gctgtggagg cgatattcgt
      241 atcgaaggtc aattaaaggg caaactcgaa tgcaaaggca aagtcgtgat tggggaatcg
      301 ggagtcgtag aaggggagat atattgccgc aatggtgatt ttgccgggaa aatcaaagga
      361 agcgtcaata cttacgaact cttaacctta aaatcaaccg ttgtgcttca aggagatatt
      421 acttgtagta aattggctat agaacctggt gcgcgtttta gtgggaaatg caccatggag
      481 cttcccgaaa acgttcaagc cgctggccaa aatgaaaaag aatgatatta aaccctcact
      541 gttcaactac gccaaatact cttcacttgc ttttcaaatg cttgtggtca tccttattgg
      601 tgttttgggt ggaataaaac tcgatcaata tctgcaattg aagttcccgg tctttactat
      661 cctgttttcg attgcagcag tgttcatagc tacttattta ggcatgaagg acttcataaa
      721 acgacctccc aaaccttctc aaaaaattga tcataatgac gaatgagttc aaacattatt
      781 tgcgcaaatt aataatcctt tctgtatttc tggctatact tggtactata ttgaagtttg
      841 tacttcccaa aggcattatt acaccggcca tgccttttat ccttatcttt tttatggctc
      901 atacgctggt ttataataat atggcagtga atatgattca tcaaaaccct aaaaaatttg
      961 tcaattttta tatgctttct actgtcggcc ggttgatttt attcgtgttg ctacttgtcc
     1021 tctatgcctt tcttgtccca aaggatttca aggccttcac cgtatgcttc ttcatttttt
     1081 acttgattta cacttttttt gaactttcta cccttttgcc tcagcttcgc aagaaaaaac
     1141 cataagtatt gtcgcgatga tctaccttac tcgccgtgaa actttcaatg cagctcacag
     1201 gcttttcagg cccgaatata gcgatgaaaa aaacctcgaa atttttggaa agtgcagcaa
     1261 tccaaactgg cacgggcata attatacctt gtatattact gtgaaaggaa tacc
//
LOCUS       DAORPD010000608         1313 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_20798_length_1313_cov_2.330684, whole genome shotgun sequence.
ACCESSION   DAORPD010000608 DAORPD010000000
VERSION     DAORPD010000608.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20798_length_1313_cov_2.330684"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /gene="coaD"
                     /locus_tag="PK976_07220"
     CDS             <1..470
                     /gene="coaD"
                     /locus_tag="PK976_07220"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HRC67713.1"
                     /translation="AVFPGSFDPITIGHHGIILRGLDLFETIIIAIGSNADKKSFFSL
                     ESRMQMISLLYDDQPRIQVEVFSGLTIDFCRSHGVRFLLRGLRTSADFEYERVVAHAN
                     RKLYPDIETVFLLAAPEHSYISSSLVKEILAYGGDASSFLPPGLKLDACLPGK"
     gene            566..>1313
                     /locus_tag="PK976_07225"
     CDS             566..>1313
                     /locus_tag="PK976_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67714.1"
                     /translation="MSDILLYGGEVVITGNFSKAAGEQVQVFTYSDFLSLNETLLAQT
                     TIDTQGYFTLEFQIEALQPILLDIAFYRQIIYVEPQKTYHIQSHRFHVLENGNPYIPR
                     YFIDAEIFSESPSDSIFRGLEYHIYNFYDTTTTRLLNQPRTEFVELFVQKILKNIPES
                     IPENYEKAILFRLASLFPKAQLPSGYSTLDEIPIDYNNYEYFRWMEDFFRKKLLIENS
                     LTASSKVSRALIMAVNKSVTYHQLQDTISKF"
BASE COUNT          362 a          276 c          239 g          436 t
ORIGIN      
        1 ctgctgtttt tcctggttcg ttcgatccga ttacaattgg ccatcacgga attattcttc
       61 gcggactcga tttgttcgaa accatcataa ttgccatagg atcgaatgca gataagaaat
      121 cattctttag cctcgagtca agaatgcaaa tgattagcct gctttatgat gatcagcccc
      181 gaatacaagt cgaagttttt tcgggcctta ctattgattt ttgccgttca catggggtga
      241 ggttcctttt gagaggctta cgaacctcgg ccgacttcga gtatgaacgt gtcgttgccc
      301 atgccaatcg aaaactttat cccgatatcg aaaccgtatt tttgcttgcc gcacctgaac
      361 actcctatat tagttcttca ctggtgaaag agatactggc ctatggcggt gatgcaagct
      421 cgtttctccc tcctggtttg aaattggacg cttgtctacc cggcaaatga atttttttaa
      481 aatttctatt ttcgaaaatt tcattcatct tcatcggaaa gcgagaaaac caaaaagaat
      541 tttattggtt ttagggctta tttttttgtc tgatatatta ctttatggcg gggaagtcgt
      601 cattacagga aattttagca aagcagccgg tgaacaggtg caagttttta cttattccga
      661 tttcctgagt ttgaatgaaa cccttctggc ccaaactacc attgatacgc aaggttattt
      721 tacactcgaa tttcagatag aagcccttca gcctatcctg ctcgatatcg cgttttatcg
      781 tcagatcatt tatgttgaac ctcaaaaaac ttaccacatt caatcccatc ggtttcatgt
      841 tctcgaaaat gggaatccct atatccctcg atacttcatc gatgctgaaa ttttttctga
      901 aagcccttct gattcaatat ttcgtggatt ggaatatcat atctataatt tttacgatac
      961 gactactact agattattga atcagcctcg aactgaattt gtcgaacttt ttgttcagaa
     1021 gattttgaaa aacatccccg aaagcattcc tgaaaattac gaaaaggcta ttctttttag
     1081 attagcatca ttgtttccca aggctcaact tccttccgga tattccacct tagacgaaat
     1141 tccaatcgat tataacaatt atgaatattt tcgatggatg gaagattttt ttcgaaagaa
     1201 gcttctcata gaaaattcac tgacagccag ttcgaaggtt tcccgggctt tgattatggc
     1261 ggtaaataaa tcggtaactt atcaccagct tcaggatacc atatcgaaat tct
//
LOCUS       DAORPD010000609         1311 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_20869_length_1311_cov_2.609873, whole genome shotgun sequence.
ACCESSION   DAORPD010000609 DAORPD010000000
VERSION     DAORPD010000609.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20869_length_1311_cov_2.609873"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1311)
                     /locus_tag="PK976_07230"
     CDS             complement(<1..>1311)
                     /locus_tag="PK976_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HRC67715.1"
                     /translation="DIQVIGATTLDEYRQYIEKDGALERRFQKVMVDPTTPEETVEIL
                     MNIKEKYEDHHLVKYTDEAIHACVYLTMRYITDRYLPDKAIDALDEAGARVHIKNIHV
                     PEEILNIEKNIEEVKQRKSDAINAQKFEEAANFRDLERKLTIELEKAKKRWEENSRIH
                     REIVSEENVAEVVAMMTGIPIQRVAQNEGARLLNMEEDLKRSIIGQDEAIRKVVRAIR
                     RNRAGLKDPNKPIGNFIFLGPTGVGKTHLAKVLAKYLFDSEDALIRIDMSEYMEKFAV
                     SRLVGAPPGYVGYEEGGQLTEKVRRKPYSIVLLDEIEKAHPDVFHLLLQVLDDGVLTD
                     SLGRRVDFKNTIIIMTSNIGSRQLKDFGQGVGFATSAKISQQNENTRSVIENALKRTF
                     SPEFLNRIDDIIIFNNLERDDIHKIIDIEMKQLYERVRNLNYEI"
BASE COUNT          323 a          295 c          271 g          422 t
ORIGIN      
        1 caatctcata attcaagtta cgaacccttt catagagctg tttcatttcg atgtcgataa
       61 tcttgtgaat atcatcgcgt tcaagattat tgaatatgat gatatcgtct atacggttga
      121 ggaattcggg tgagaatgtt cttttcagtg cattttcgat gacgctgcgt gtattctcgt
      181 tttgctgaga aattttagca ctggtagcaa atcccactcc ctggccaaaa tctttcaact
      241 gacgcgaacc aatattagaa gtcatgataa taatagtatt cttgaagtcg actcttctac
      301 ccaaactgtc ggtgagcacg ccatcatcta aaacttggag caggagatga aatacatcgg
      361 gatgagcctt ttcgatttcg tcgagcagga caatggaata aggtttacga cgaactttct
      421 cggttaactg acctccttct tcataaccaa cataacccgg aggcgcacca accaagcgtg
      481 ataccgcaaa tttctccata tattcactca tatctatacg gatgagagca tcttccgaat
      541 cgaacagata tttagccaga acctttgcca gatgggtttt cccaactccc gtgggaccca
      601 ggaatataaa gtttcctata ggtttatttg gatccttgag tccggcgcga ttgcgacgga
      661 tggcacgcac tacctttcgt atggcttcat cctgtccgat gatgcttctc ttgagatctt
      721 cctccatatt taaaagacgc gcaccctcat tttgagccac tctttgaata ggaatgcccg
      781 tcatcatggc tacgacttct gcaacatttt cctcgctaac aatttcacgg tggatgcggc
      841 tattttcttc ccagcgtttt ttagcttttt cgagctcgat agttagtttg cgttccagat
      901 ctctgaaatt ggccgcttct tcgaactttt gggcattaat ggcatccgat ttacgctgct
      961 taacttcttc gatgttcttt tcgatattga gaatttcttc gggcacgtgt atgtttttaa
     1021 tatgcacgcg tgcaccggct tcgtcgaggg catcgatggc cttatcggga agataacggt
     1081 cggtgatata ccgcatggta agataaacac aggcgtgaat agcttcatcg gtgtatttta
     1141 ccagatgatg atcctcgtat ttttctttga tgttcattaa aatctcgacg gtttcttcgg
     1201 gtgtggtagg atcgaccatc actttttgga aacgtctttc gagagcccca tctttttcga
     1261 tatattgacg atattcatct aaggtagtgg ctccaattac ttgtatatca c
//
LOCUS       DAORPD010000610         1309 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_20940_length_1309_cov_1.942584, whole genome shotgun sequence.
ACCESSION   DAORPD010000610 DAORPD010000000
VERSION     DAORPD010000610.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20940_length_1309_cov_1.942584"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            50..430
                     /gene="rpsM"
                     /locus_tag="PK976_07235"
     CDS             50..430
                     /gene="rpsM"
                     /locus_tag="PK976_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HRC67716.1"
                     /translation="MARIAGIDLPKNKRGEIGLTYIYGIGRSTSRKILSEAGIDYDKK
                     VAEWNDEELSKVRNIITNHYKVEGALRSEVQMNIKRLMDIGCYRGIRHRLGLPVRGQA
                     TKNNARTRKGKKKTVANKKKATKG"
     gene            473..871
                     /gene="rpsK"
                     /locus_tag="PK976_07240"
     CDS             473..871
                     /gene="rpsK"
                     /locus_tag="PK976_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007567382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HRC67717.1"
                     /translation="MAKSTKTVKSTKKRVVKVDATGRVYIHSSFNNIIISLTNNNGEV
                     ISWASAGKMGFRGSKKNTPYAAQIAAQECAKVAYDLGLRKVKAYVKGPGAGRESAIRT
                     IHSSGIEVTEIRDITPLPHNGCRPPKRRRV"
     gene            909..>1309
                     /gene="rpsD"
                     /locus_tag="PK976_07245"
     CDS             909..>1309
                     /gene="rpsD"
                     /locus_tag="PK976_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HRC67718.1"
                     /translation="MARYIGPKTKIARRFRDPIYGPDRYFEKKNYPPGQHGQSRRRGK
                     QSEYGMQLAEKQKAKYTYGILERQFRNIFQKAARMRGITGEKLLQLIESRLDNVVFRL
                     GIAPTRASARQLVSHRHIMVNGKIVNIPSYL"
BASE COUNT          460 a          247 c          294 g          308 t
ORIGIN      
        1 gtcagggata gacattaaac atataacata ataaaatcaa gaataaagta tggcaagaat
       61 tgcgggtatc gatttaccga aaaacaaaag aggcgaaatt ggcctcacct atatttatgg
      121 tattggtcgc tctacttcgc gaaagattct gagcgaggct ggaatcgact atgataaaaa
      181 agtggctgag tggaacgacg aagaactttc caaagtacgg aacataatta ccaatcatta
      241 taaggtggaa ggagctcttc gttcggaagt gcaaatgaat ataaaacggc taatggatat
      301 tggttgttac agaggcattc gtcatcgcct gggattgccc gtacgcggcc aggcaaccaa
      361 aaacaatgcc cgcacacgca aaggcaagaa aaaaaccgtt gccaataaga aaaaagctac
      421 aaaaggatga tatcataatg gaaattgatt ataataaagt aaaataaagg aaatggcaaa
      481 gagcactaaa actgtgaaat ccaccaagaa aagagtcgta aaggtggatg caaccggaag
      541 agtatacatt cattcgtcgt tcaacaacat tattattagc cttaccaata acaatggtga
      601 ggttatatcg tgggcttcgg cggggaagat ggggtttaga ggttcgaaaa aaaatacacc
      661 ctatgctgct caaatagctg cacaggagtg tgccaaagta gcttacgatt tgggattacg
      721 aaaggtaaaa gcttatgtaa aaggaccggg tgcaggtcga gaatctgcca tacgaaccat
      781 tcattcttcg ggcatcgaag ttaccgaaat ccgcgatatt actccattgc cacataatgg
      841 ctgccggcct cctaaaagaa gacgcgtttg attcttcgaa tataaaaata tgttaaaata
      901 taatccttat ggcaagatac attggtccaa agactaaaat agcaagaaga ttcagggacc
      961 cgatctatgg tcctgataga tatttcgaga agaagaatta tcctcctggc cagcatggtc
     1021 aaagccgacg ccgtggaaaa caaagtgaat atggtatgca attagccgaa aaacaaaagg
     1081 caaagtatac ctatggaatt ctcgagcgcc agttcagaaa tatatttcaa aaagcagccc
     1141 gtatgagagg aattaccggc gagaaattgc ttcagctcat agagtcacgt ctcgataacg
     1201 ttgtattcag attaggcatt gcacctacga gggcttcggc tcgccaactg gtttcgcacc
     1261 gtcatatcat ggtaaatggc aaaatcgtga atatcccttc ctatttatt
//
LOCUS       DAORPD010000611         1307 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_20985_length_1307_cov_2.204473, whole genome shotgun sequence.
ACCESSION   DAORPD010000611 DAORPD010000000
VERSION     DAORPD010000611.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20985_length_1307_cov_2.204473"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /gene="tsaB"
                     /locus_tag="PK976_07250"
     CDS             <1..633
                     /gene="tsaB"
                     /locus_tag="PK976_07250"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HRC67719.1"
                     /translation="QLMGLEETEDKNAHSARLTVLIEKLLKDNNLKMSQLDAVAMSRG
                     PGSYTGLRIGASVAKGLCYGLNIPLIAVSTLQAMALQAARILQISPQEDVVFIPMIDA
                     RRMEVYSAVYNFRGEELREVRAEIITADLFEDIHSRIVICGDGAAKCRQLFENDLRFI
                     IMERLLASAAWLVPLAWENFVKGKFENTAYFEPYYLKDFIAGIPHIKGLK"
     gene            630..1235
                     /locus_tag="PK976_07255"
     CDS             630..1235
                     /locus_tag="PK976_07255"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HRC67720.1"
                     /translation="MNIEKNVNLAIFASGSGTNAENIIKYFKDHGYIRVIRVYCNNPQ
                     AYVINRVKPYEIPVTLFGKEDFVENGLVHQKLKEELIDYIILAGFLWKIPDFLIQQFS
                     GRILNIHPALLPKYGGKGMYGERVHQAVLAAGETESGISIHRVDEAYDHGEIVFQFKC
                     PVLPEDTPETLANRIHKLEYEYYPKIIEEVIVSDGKRQTTL"
BASE COUNT          386 a          213 c          297 g          411 t
ORIGIN      
        1 cagcttatgg gattggaaga gaccgaagac aagaatgctc attcggcgcg gcttacggtt
       61 ttaattgaaa aattattgaa agataataac ttaaagatga gtcaattgga tgcagttgcc
      121 atgagtcgtg gtccgggttc ttatactgga ttgcgtattg gggcttctgt tgcaaagggt
      181 ttgtgctatg gtttgaacat tcccttgatt gccgtgagta ctttacaggc tatggctttg
      241 caagccgcaa gaattttaca gatttctcct caagaggatg tagtttttat tcccatgata
      301 gatgcgagaa ggatggaagt atattctgct gtttataact ttaggggtga agaactaaga
      361 gaagtaaggg cagaaattat tactgcagat ttatttgagg atattcattc cagaattgtt
      421 atttgtggag atggagcagc aaagtgccgg caactttttg aaaacgatct gcggttcatc
      481 attatggaaa ggcttctcgc ctctgctgct tggttagttc ctctggcctg ggaaaatttc
      541 gtgaagggaa agttcgaaaa taccgcttat ttcgaacctt attatttaaa ggattttatt
      601 gccggaatac ctcatatcaa agggctgaaa tgaatataga gaaaaatgtt aatctggcta
      661 tatttgcttc gggcagtggt actaatgccg aaaatatcat aaaatacttc aaagaccatg
      721 gatatattcg ggtaataagg gtttattgca acaatcctca agcttatgtg ataaatcgtg
      781 taaaaccata cgagattccc gttaccctct ttggtaagga agatttcgtc gaaaatggct
      841 tagtgcacca aaaactgaaa gaagaactca tcgattatat cattcttgca ggttttttat
      901 ggaaaattcc tgattttctc attcaacaat tttctgggcg cattcttaat attcatcctg
      961 ctcttttgcc taagtatggc ggcaaaggta tgtatggcga aagggttcac caggcggttt
     1021 tggctgctgg agaaacggaa agtggcatta gcattcatcg tgtcgatgaa gcgtatgatc
     1081 atggagagat agtatttcag tttaaatgcc ctgttttgcc agaagatacg cctgaaaccc
     1141 ttgcaaatag aatccataag ctggaatatg aatattatcc gaaaattatc gaagaagtaa
     1201 ttgtctcaga tgggaagcgc caaaccacac tttaatattc cttgagattc ttgttaattt
     1261 tttgaggtat aattggttta tcaggcacat ttatttcaac gaaggat
//
LOCUS       DAORPD010000612         1306 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_21018_length_1306_cov_2.254197, whole genome shotgun sequence.
ACCESSION   DAORPD010000612 DAORPD010000000
VERSION     DAORPD010000612.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21018_length_1306_cov_2.254197"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="PK976_07260"
     CDS             complement(<1..188)
                     /locus_tag="PK976_07260"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HRC67721.1"
                     /translation="MNNDDAAPLKKVIEELLETYNLKSGFTQVRIQRIWSQIVGRHIA
                     KRTSEIRLKNDTLFVRLNS"
     gene            complement(273..>1306)
                     /locus_tag="PK976_07265"
     CDS             complement(273..>1306)
                     /locus_tag="PK976_07265"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRC67722.1"
                     /translation="GTLDDFRALVKNAHELGMYVIVDWVANHSAFDCPLASEHPEWYR
                     HAQDGSFISPYDWTDVIAFDYSNLHLRKYMTEAMKWWLKETSIDGFRCDVAGLIPVDF
                     WEDTRKALDSIKPVFMLAEADTAALMFNAFNAGYNWGLLDRMIRIYQGKIKANQILAF
                     IKKYPDSYPQGSFKMNFTSNHDINSWTDTEQELFGEADLTMAVLTATVPGMPLVYSGQ
                     EEPADKRLRFFDKDTIQFNNYSNQEFYRCLLELKKRNPALWHGGNHDRIIPVQSSDSI
                     HALVFMRENKNNKILVVLNLSDQPLTLTITDKKAEGKYNEIFLNTSVNISRTEPLELQ
                     PWGYRVLEK"
BASE COUNT          382 a          262 c          259 g          403 t
ORIGIN      
        1 gaattcagcc taacgaataa agtatcgttt ttcaagcgga tttcgcttgt tctttttgca
       61 atatgccgtc ctactatctg gctccagatt cgttgaattc tcacttgggt aaaacccgat
      121 ttcagattgt atgtttcaag aagttcttct atcacctttt tgagaggtgc agcatcatca
      181 ttattcatcg gtacgattgg aaaataattc tattcaacat tcggttcata agaaagcaca
      241 ctcagaagca aaaagggcat acacaaaact tattattttt cgagtactct gtatccccag
      301 ggctgaagtt ctaagggttc ggtccttgaa atgtttacag atgtatttaa aaaaatttca
      361 ttatacttac cctcagcctt tttatcggtg atagttagag taagcggttg gtcactaagg
      421 tttaatacca ccagaatctt attgttttta ttttcacgca taaataccag agcatgaata
      481 gaatcggatg attgaacagg gatgatccta tcgtgatttc cgccatgcca gagagctgga
      541 tttcgctttt tcaattctaa gagacaacga taaaattcct gatttgaata attattgaat
      601 tgaatggtgt ctttatcgaa aaaacgtaag cgtttgtctg caggttcttc ttgaccgcta
      661 tatacgagag gcatgcctgg gacagtagct gtaagtacag ccatagttaa gtctgcttct
      721 ccaaatagtt cctgttcggt gtctgtccat gaattgatat catgattcga agtaaaattc
      781 attttgaaac taccttgagg gtagctgtca gggtatttct ttataaatgc aagaatttga
      841 ttagctttaa tttttccctg ataaattcga atcattctat cgagcaagcc ccaattgtat
      901 ccggcattga aagcattgaa catcaaagca gcggtatcgg cttctgcgag cataaataca
      961 ggtttgatgc tatcgagggc ttttcgtgta tcttcccaga aatcaactgg aataaggcca
     1021 gccacatcgc agcgaaaacc atcgatcgaa gtttccttca gccaccattt catggcttcc
     1081 gtcatatatt tccgtaaatg caggttggaa taatcgaagg caattacatc tgtccagtcg
     1141 tagggagaaa taaaactacc atcctgagca tgcctatacc attcgggatg ttctgaggca
     1201 agaggacaat cgaaagccga gtggttggca acccagtcaa caatgacata catacccagt
     1261 tcatgggcat tttttaccag agccctgaaa tcgtctaaag tgccaa
//
LOCUS       DAORPD010000613         1303 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_21096_length_1303_cov_2.129006, whole genome shotgun sequence.
ACCESSION   DAORPD010000613 DAORPD010000000
VERSION     DAORPD010000613.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21096_length_1303_cov_2.129006"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            385..729
                     /locus_tag="PK976_07270"
     CDS             385..729
                     /locus_tag="PK976_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67723.1"
                     /translation="MVLIPGYVFMRTNEVAICSDEFHLPDSRDRLIQDEKPALLSHDN
                     AMNLNRLCNLDLAPEITTIFGCEQQITIDSGSLKGISGKVIRTDGKQYLIVENGIKDI
                     LLKVNLEQNALI"
BASE COUNT          404 a          241 c          272 g          386 t
ORIGIN      
        1 gttgcatgaa taaacaccca tacaatgttg aggttcttcc ttttgtgatg ttttatccct
       61 tgcccttttt ctccattgat ggaagaaaaa ttttcatcac agtttgattt gaaaattcta
      121 tttatatttc ctcgttgaag atgtatcaca ttgtgagcag gcaaaaatat atcctcttgt
      181 tgggatgtac aattccaaca taaagcggag ttataagccc ggcacatacc ccgatgattc
      241 tgtgccaaag tcatggctca tcctgcgttc tctggcaaag aaagagagaa ttttggtagt
      301 acagcttcta cgaaaggttt cgatgcctaa ctacccgaaa ttaaacattg gcgttttcgt
      361 gcgggaaaga aagaaaatat tattatggta ctcattccgg gctatgtatt tatgaggact
      421 aatgaggtgg ctatttgttc cgacgagttc catctgccgg atagcagaga ccggttgata
      481 caggatgaga aacctgcact gttgtctcac gataatgcaa tgaatctgaa tcgtttatgt
      541 aacctcgatt tagcccctga aataaccact attttcggtt gtgaacaaca gattacgatt
      601 gattccgggt ctttgaaggg aatttcgggc aaggtcattc gtaccgatgg caagcaatat
      661 ttgatagtgg aaaacggcat caaggatata ctgctgaaag tgaatctgga gcagaatgcg
      721 ttgatctaag aaattagcat acaatgaaag tattagggca gaaaataata atcctttgcg
      781 aattaagtac ccacagaaat aacgatgtag tatttctgaa tttcgaattt gataaaactc
      841 tgaatgaaat agtgcgaagg cttggctgtg ccagatggag ccagacaaac cttaaatgct
      901 atcttccctg ccaggaattt aaccttgcca ggcttcggaa ggcgatggac ggtttggcag
      961 atatagatgt ttcctaatta aatgaaaaca tggagacact tcagaaacca aaaggctccc
     1021 cttcgtataa tattcaggac gagttgaata aattcaatgc gtggcttttg tacaggcggt
     1081 atagccataa ttcgattaaa cttatattga tgcactaaaa aactttctac tattcctcca
     1141 acccaaaaca gcttccaatg caaccactaa cgacatggtg cgctttgtta acgattacat
     1201 tttaaaaaac ggatatagtc tttcgtttca gaatcaagtt gtaaatgcgt gcaagcttta
     1261 ctttaaagaa gtcgcgcata gcgagttgga tgttaataat ttg
//
LOCUS       DAORPD010000614         1302 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_21125_length_1302_cov_2.797915, whole genome shotgun sequence.
ACCESSION   DAORPD010000614 DAORPD010000000
VERSION     DAORPD010000614.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21125_length_1302_cov_2.797915"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /gene="cysD"
                     /locus_tag="PK976_07275"
     CDS             complement(<1..783)
                     /gene="cysD"
                     /locus_tag="PK976_07275"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006283813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009336 - sulfate adenylyltransferase
                     complex (ATP) [Evidence IEA];
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA];
                     GO_process: GO:0070814 - hydrogen sulfide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HRC67724.1"
                     /translation="MGEYKALFTIKMNHLRELEAEAIYIIREVAAQFERPVILFSGGK
                     DSIVVVHLAYKAFWPAPLPFPLLHIDTGHNFHETIEYRDALVKKTGVRLIVGSVQETI
                     DNGKVKEETGYNASRNVLQTVTLLETIEEYKIDAAIGGGRRDEEKARAKERVFSHRDE
                     FGQWDPKNQRPELWNIFNGKKHLGEHFRVFPISNWTELDVWQYIYYERIEMPSLYFTH
                     KRKVFCRDGVWLAYAPFMKLKPDERVEEREVRCRTIGDITCTG"
     gene            complement(1110..>1302)
                     /locus_tag="PK976_07280"
     CDS             complement(1110..>1302)
                     /locus_tag="PK976_07280"
                     /inference="COORDINATES: protein motif:HMM:NF020922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2461 family protein"
                     /protein_id="HRC67725.1"
                     /translation="PKGFPENFADIEILKNKHFTVARLLSDEEIIESDFPDKVLEIFK
                     ALQPLNHFFNGPVEDIILS"
BASE COUNT          390 a          291 c          232 g          389 t
ORIGIN      
        1 ccccgtacag gtaatatcac caatagtgcg gcagcgaact tcacgttctt cgacccgttc
       61 gtcgggtttc agtttcataa atggcgcata ggccaaccaa actccatcac gacaaaaaac
      121 ttttctttta tgcgtaaaat ataaactggg catttctatt ctctcgtaat aaatatactg
      181 ccacacatcc aattcagtcc aattgctgat ggggaaaacc ctgaaatgtt cacccagatg
      241 ttttttccca ttgaagatat tccaaagctc gggtcgttga ttttttggat cccattggcc
      301 aaactcatcg cgatgtgaaa aaacgcgttc cttggctcgg gctttttctt cgtcacggcg
      361 cccccctcct atggcagcgt caattttata ttcctcgatg gtttccagta gggtaacggt
      421 ttgaagcaca ttgcgactgg cattataacc tgtttcttcc ttaacctttc cattgtcaat
      481 ggtttcctgc accgacccaa caatcaaacg aacacccgtt ttttttacaa gggcatccct
      541 atattctatt gtttcgtgaa aattatgtcc cgtgtcgata tgcagaagag gaaaaggcag
      601 aggagccggc cagaaggctt tataagcaag atggactaca acgatagagt ctttcccacc
      661 tgaaaaaagg atcacaggtc tttcgaattg agcagcaact tcgcgaatga tatagatggc
      721 ttcagcttcg agctccctca aatgattcat cttaattgta aataaggcct tatattctcc
      781 cataataaaa tttcgtatgc aaatttacga aaccaaaagg cttcaaaagt atatgatgat
      841 tcagaaatgg gttataggta attttaaaga caagagattg gaagaaatgc caatgcctga
      901 acatggttag ccgttaaaga caaatctaat ttttttggat gtagaggccc tcgaagcctc
      961 catcttatca ttcccatcca aaatctattt ctatcataaa atgccttgta aatcaattct
     1021 atagctaaag tcggaaaact tccccaaagg tctatttcac tcattatcaa cactccaaac
     1081 gcaccacgcc gatcaaggta atccttcgtt cagctcagaa tgatgtcttc aacaggtcca
     1141 ttgaaaaaat gattcaatgg ctgtaaggct ttgaatattt ctaatacttt atcaggaaaa
     1201 tctgattcaa taatttcttc gtcagaaagc aggcgagcca ccgtaaaatg tttattcttt
     1261 aaaatttcta tatctgcaaa attttctgga aatcctttgg gt
//
LOCUS       DAORPD010000615         1301 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_21164_length_1301_cov_2.090690, whole genome shotgun sequence.
ACCESSION   DAORPD010000615 DAORPD010000000
VERSION     DAORPD010000615.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21164_length_1301_cov_2.090690"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(297..>1301)
                     /locus_tag="PK976_07285"
     CDS             complement(297..>1301)
                     /locus_tag="PK976_07285"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HRC67726.1"
                     /translation="NVYADGVYVGLHPLGPFQYQDLNPFSYKPGGFARGAGHGATFCD
                     YNQQWWHISTITIGVKNNFERRIGLWPAYFDPDGILYAETAFGDFPHYYNGKFTGWML
                     LNYQKPVTVSSTLGGYYPNFAVDEDIRTYWSAKSGKAGEWICTDLSDTCVVYAFQINY
                     ADQDVPFLGKTSGLNYQYRIWGSLNGKDWTMICDKSDNRTDVPHDYVALESPVELRYL
                     KLENLHIPGGKFALSGLRIFGKANILPPTQVEYFTILRGHSEPRNAWLKWNRKDDATA
                     YLIRFGLEPDKLYGSIMVFGENEYYFTGMDRNKTYYFTIQAFNEGGCGPQSQTVESSP
                     "
BASE COUNT          371 a          295 c          240 g          395 t
ORIGIN      
        1 tcagctccca ctcaggatat tttacaacta tccctcaaaa ccaattccat cttgataatt
       61 ttcccgatat gccttcttta ccttgctttc ataccataaa cagggaatat ctcgataagg
      121 cttgtgaaat ggtaataggc ttgctttaat aaagggaaaa ccgccttttc gatttttcat
      181 ttctccagag ttggattcaa tacctttctc gcagcttcat atcgaataga taaacaagat
      241 ttatacgaag cgatcgaaag tttcggcaga agaaccttga aaagtaatct ttatctttag
      301 ggagatgatt ctaccgtttg gctttgtggt ccacaacctc cttcattaaa ggcctgaatc
      361 gtaaaataat aggttttgtt tctgtccatc ccggtgaaat agtattcgtt ttcaccaaat
      421 accatgatgc ttccatataa tttatccggt tccagtccga aacgaatgag ataagctgtt
      481 gcgtcatcct tgcgattcca tttcaaccat gcattgcggg gttcggaatg accccttaga
      541 attgtaaaat attctacctg agtgggtggt aaaatattag ccttcccaaa tattcgcaag
      601 ccactcaagg caaattttcc tccaggtatg tgaagatttt cgagcttgag atatcgtagt
      661 tcgacgggcg attcgagtgc gacataatcg tgaggcacat cagttcgatt atcagattta
      721 tcgcatatca tcgtccaatc ttttccattc aggctgcccc atattctata ctgataattc
      781 aagcccgaag tttttcctaa aaatggtaca tcctgatcgg cataatttat ttgaaaggca
      841 taaaccacac aggtatcgct caaatctgta cagatccatt ctcctgcttt tccgcttttg
      901 gcactccaat aggtacgaat gtcctcatca acggcaaaat tgggataata gccgcctaaa
      961 gtactcgata cggtcacggg tttctgataa ttcaacaaca tccatccagt aaattttcca
     1021 ttgtaataat gaggaaaatc tccaaaagct gtttcggcat aaaggattcc atcggggtcg
     1081 aagtaggctg gccataagcc tattcttcgt tcgaaattgt tctttacccc gatggtgatg
     1141 gtcgaaatat gccaccattg ctggttataa tcgcaaaatg tggctccatg cccggctccc
     1201 cttgcaaaac ctcctggttt ataactaaat ggattgagat cctgatactg aaatgggccc
     1261 aagggatgca atcccacata aactccatcg gcataaacat t
//
LOCUS       DAORPD010000616         1299 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_21233_length_1299_cov_2.299035, whole genome shotgun sequence.
ACCESSION   DAORPD010000616 DAORPD010000000
VERSION     DAORPD010000616.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21233_length_1299_cov_2.299035"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            138..263
                     /locus_tag="PK976_07290"
     CDS             138..263
                     /locus_tag="PK976_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67727.1"
                     /translation="MKVLNEEFKKEITAADYKAATIDAAMDFEMRTIKIYSSRAK"
     gene            complement(475..1176)
                     /locus_tag="PK976_07295"
     CDS             complement(475..1176)
                     /locus_tag="PK976_07295"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HRC67728.1"
                     /translation="MKQKKISRLFFILIISFSVFISKESEAQVFRGILIGGFNLSQVD
                     GDETYGFHKLGANIGAGVMLPFTRKWSVTLETFLSQKGSHQDAFYVDTLPTGNILTGE
                     YDLRMNYVEIPLLIHFTDKETVSAGVGVSYGRLIGVTEKEHGKKVESTSVNNGPYKKD
                     DWDIIGDIKLPLYKNLKINLRYSYSLAKIRTRDFYDRNGEYLKTRDQYHNLFSIRFLW
                     VINEKTRTLQEQKNE"
BASE COUNT          394 a          255 c          247 g          403 t
ORIGIN      
        1 ggtaaaaata attacggcaa tattggaagt gggtatgagc atttacatta tctctcaact
       61 cattttcacc cttttgccaa atctccgaat ttttaaaacc cgaagttatt tccaccaata
      121 aggaagaaga tttagccatg aaggttctga atgaagaatt caagaaagag ataactgctg
      181 ccgactataa agcagccact attgatgcgg caatggattt cgaaatgcga accataaaga
      241 tttattcaag ccgtgctaaa tagttggacg agcctcatga agaagaattg taccgaattc
      301 atgctggttg caggaaaggc catcatgagt tgctcgaacg gattaatgat gaagtgaaaa
      361 gtaccaatat agaattataa ccgattctgg ccattctgaa aaactttcac actcgattca
      421 ttcttatttt acttgccttt gcttgttatc ctgatgcagc cctcactgat taggctactc
      481 attcttctgt tcctgcaagg tacgtgtttt ttcgttgatg acccacaaaa aacgtattga
      541 gaacaggttg tggtactggt cacgggtttt gaggtattcg ccattacgat cgtagaaatc
      601 ccgtgtgcga atcttggcaa gcgaatacga atatcttaga ttgattttta gatttttata
      661 aagtggtaac ttaatatctc caataatatc ccagtcatct tttttataag ggccattatt
      721 tacagaagta gattctactt ttttgccatg ttccttttcc gtaacgccta tcaaacggcc
      781 atagctaaca cccacgccgg cacttacagt ttccttatcc gtaaaatgaa taagtaaggg
      841 tatttcgacg taattcattc tcaaatcata ttctcctgtc agaatattac ctgtggggag
      901 cgtatccaca taaaacgcat cttgatgaga tcctttctgt gaaagaaacg tttcgagggt
      961 caccgaccat tttcgggtaa agggcaacat tacccctgct cctatatttg ctcctaactt
     1021 gtggaagccg taggtttcat ctccatcaac ttggcttagg ttgaagcctc ctataagaat
     1081 acccctgaat acttgggctt ctgattcttt tgaaatgaac accgaaaaac taataattag
     1141 gataaagaat aaccgggaaa tttttttttg cttcatagaa agacggcttt gatagcaaca
     1201 aattttatta gtgatgtagc gaaaattttt gcaatcgagt tgttcgttcg acgaataaat
     1261 tcgttggcaa aagtaccgaa ttaaaaccat taggccgaa
//
LOCUS       DAORPD010000617         1298 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_21257_length_1298_cov_2.423170, whole genome shotgun sequence.
ACCESSION   DAORPD010000617 DAORPD010000000
VERSION     DAORPD010000617.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21257_length_1298_cov_2.423170"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(331..939)
                     /locus_tag="PK976_07300"
     CDS             complement(331..939)
                     /locus_tag="PK976_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67729.1"
                     /translation="MKKINLAVCILCLGALSICSSSCKQNPKANSSGEVEKTNQQKSP
                     EFKYDDLDRIVQRKDFSYNRDGSVRYKNQYDYKYDDRGNRIEEITQSWDLSGKRVVYT
                     IARFHFNDLNLKIEAIFQSFDPEGREILWVKNVYDYDDNGYEIRDQQFDKQGRPLSLV
                     TRKRDEKGRILEETTTIYNPDGSIKEHKGAQYDINGRILKTW"
     gene            complement(1002..>1298)
                     /locus_tag="PK976_07305"
     CDS             complement(1002..>1298)
                     /locus_tag="PK976_07305"
                     /inference="COORDINATES: protein motif:HMM:NF024687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HRC67730.1"
                     /translation="SKHAYRVVEARWFEKAGEGPYQALLRITGDDRIGLLADITHVLT
                     NELNVNILSVNLDSKDGFFDGKMKVQVTNVAHLDSVIHRLMKVKSVARVKRFYD"
BASE COUNT          399 a          257 c          220 g          422 t
ORIGIN      
        1 ttattcatga aattattttg ttatcggaaa aaatctttca tgccatttca agcatcaatc
       61 atcatgaatt caaacgacca tgttgttcga atgataatga aggaacatgc catgcttctc
      121 ataccagatt agcaggtaat tcaacaaaat gaatcaaaaa tagaatcata aatttatttt
      181 tatgataggt aatttaatga agttaatatt ttcatttcct gaaatacaac ataaccacaa
      241 gaaaagtcaa ttaattcatt cattgatgtt gaatatttca tttcgtttga tttttgcagt
      301 tatttaggaa caggtgtaaa aaaatttatg ttaccaagtt ttcaaaattc taccattgat
      361 gtcgtactgg gcacctttat gttcctttat gcttccatca ggattataaa tagtagtagt
      421 ctcttcgaga attctgccct tttcgtcgcg ttttcgggtc actaatgaaa gaggccgccc
      481 ttgtttatcg aattgttggt cacgaatttc atatccatta tcatcataat catatacatt
      541 tttcacccat aagatttcac ggccttcggg atcgaatgac tgaaaaatgg cctcaatttt
      601 cagattcaag tcattgaagt gaaaacgggc aatggtatag accacgcgtt taccactcaa
      661 atcccacgat tgcgtaattt cttcgattcg gttgcctcga tcgtcgtact tataatcgta
      721 ctggttctta taacgcacag aaccatcacg gttataggaa aaatcctttc tttgcacaat
      781 acggtcaagg tcgtcgtatt tgaattcggg agatttttgt tggttcgttt tctctacttc
      841 acccgatgag tttgccttag ggttttgttt acatgaagaa gaacaaatac ttaatgcacc
      901 taagcacaaa atacatacag ccaggtttat ttttttcata gcccacatac aatataagtg
      961 tttgaaggcg caaaattaaa aaattaagcc ttttaaaatt attaatcata gaagcgtttc
     1021 accctagcca cactttttac cttcataagc cgatgaataa ccgaatcgag atgagcaaca
     1081 tttgtcacct gcactttcat ctttccatcg aagaatccat ctttcgaatc gaggtttaca
     1141 gagaggatat tgacattcag ttcattggtc agaacatggg tgatatcggc cagaaggccg
     1201 attcgatcgt cgcccgtaat acgaagcaaa gcctgataag ggccttcacc agctttttcg
     1261 aaccagcgag cctcaactac cctgtaagca tgtttcga
//
LOCUS       DAORPD010000618         1298 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_21259_length_1298_cov_2.341110, whole genome shotgun sequence.
ACCESSION   DAORPD010000618 DAORPD010000000
VERSION     DAORPD010000618.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21259_length_1298_cov_2.341110"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="PK976_07310"
     CDS             <1..223
                     /locus_tag="PK976_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HRC67731.1"
                     /translation="GPQIGIKKSCAQLTVNYSLKDLIGKLVLCVVNIQARQIGHFISE
                     VLTLGVPDIKGECILITPDRNVPLGSRLY"
     gene            complement(518..>1298)
                     /locus_tag="PK976_07315"
     CDS             complement(518..>1298)
                     /locus_tag="PK976_07315"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRC67732.1"
                     /translation="LGSSSKVDLLQSIVYLNSDSSRFSKQNEVLRTSQIRLKKLMGEN
                     QIEGEFLVIDTSIPINANLDYDELLDNTLSHNTALQIASKNKTITGLDYQIISSRSYP
                     YLNLTAGYNYSYNGYETGSLKNQQVYGFNYGLTLGMNVFDGSNQKREKANARLEVENK
                     EHNYDDILLQIKADLVTIYYAYQNNLKLLQLEQQNLAVARENLEIAMERYKLGNLSGL
                     ELREVQKSLLDAEERLISVKYQTKLAEISLFQISGKLLEYL"
BASE COUNT          382 a          245 c          223 g          448 t
ORIGIN      
        1 gggtcctcag attggaataa agaagtcgtg tgcccaattg acggtaaact actcattgaa
       61 agacctcata ggaaagctgg tgttatgtgt ggtaaacata caagctcggc aaatcgggca
      121 tttcatttca gaagtattga cattaggcgt tcccgatata aaaggagagt gcattctcat
      181 tactccagat aggaatgttc ctttgggttc gaggctttac taaatgaagc tgattaatta
      241 cgttatttgg aatagaaatt cattttttta tttttctgaa aataaatcac ttaaacacta
      301 taatcaccct tcatggagtc tgaagaaggt gggtttatgt cgaaattatt aataatccat
      361 cctaagaccg gctcaatcag aaattttatc ctgtttgcat aatttacctt cctcaaatcc
      421 tttcctccat ttctccgttt ttttaattta taagctttta actttcaact ttttaaattc
      481 tattcatcat tcaccatttt tctcttagac ttacttacta caagtactca agtaattttc
      541 ctgaaatctg gaataaggaa atttcggcca gcttggtttg atatttcacc gaaatgagcc
      601 gctcttcggc atcgaggagg cttttttgaa cctctcttaa ttcgagcccc gataaattgc
      661 ccaatttata tcgttccatg gcaatttcga ggttttcacg ggctacagct aaattttgct
      721 gctcgagctg gagcaatttt aaattattct gataagcata gtaaattgta actaaatcgg
      781 cctttatctg aagcaaaata tcatcgtaat tatgctcctt attttcgacc tcgagtcgtg
      841 cattggcttt ttcacgtttt tgattggaac catcgaacac attcatgccc aacgtcaatc
      901 catagttaaa tccgtaaacc tgttgattct tcaaactacc tgtttcgtac ccattatagg
      961 aataattata tccagccgta agattcaaat aaggatatga acgtgaagag atgatttgat
     1021 aatcaaggcc ggtaatagtt ttattcttcg aggcgatttg cagagctgta ttatggctta
     1081 aagtattatc gaggagttca tcataatcaa gattggcatt tataggaata cttgtatcta
     1141 ttacgagaaa ttcaccttca atttggtttt ctcccatcaa tttcttcaat cggatttgag
     1201 aagttctcaa tacttcgttt tgttttgaaa acctcgaact atcggaattc aaatacacga
     1261 tggattgcaa gagatcaact tttgacgaag atcctaat
//
LOCUS       DAORPD010000619         1295 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_21328_length_1295_cov_2.185484, whole genome shotgun sequence.
ACCESSION   DAORPD010000619 DAORPD010000000
VERSION     DAORPD010000619.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1295
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21328_length_1295_cov_2.185484"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1295
                     /locus_tag="PK976_07320"
     CDS             <1..>1295
                     /locus_tag="PK976_07320"
                     /inference="COORDINATES: protein motif:HMM:NF024724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRC67733.1"
                     /translation="GEPYSYHPLEVARICAEEIGLGATSIAAALLHDVVEDRSDLYSV
                     EDIHRLFGDKIARICEGLTKIKEIFDHTQPSDQAEHYRKLLLTLSDDVRVILIKLADR
                     LHNMRTLQYMRPEKQLKIAAETTYLFAPLAHRLGLYSIKSEMEDLALKFTEPEIYKEI
                     SRRMAESDPVRANFAEEFIKPIEKDLKEKGFRYRIMRRNKSVYSIWTKMKKKHIPFDE
                     VYDVFAIRIILDVPINDERSACWQAYSIVTSHYRPKPDRLRDWISIPRANGYQALHTT
                     VMSKEGKWVEVQIRSERMDEIAEKGYAAHWKYKGQYYDDKNIDNWLDRIRDMLQNGEA
                     GALDFLEDVQGYLFSSEIFVFKLDGELRILPAGSTVLDFAYAINPDEGNHCIGANVNH
                     KLQNRFYELKSGDQIEILTSKIQEPSEDWLVHVKTSRAK"
BASE COUNT          400 a          301 c          270 g          324 t
ORIGIN      
        1 gtggcgagcc ttatagttat catcccttgg aagttgcccg catttgtgcc gaagaaatcg
       61 gactgggagc tacttctatt gctgctgcct tgctccacga cgttgtggaa gaccgctccg
      121 acctatattc cgttgaagat atccatcgac tcttcggaga taaaatagct cgcatctgcg
      181 aaggtttaac caaaatcaag gaaattttcg atcacaccca accttccgac caagccgaac
      241 attatcgcaa acttctgctt actctttccg atgatgtaag agtaatcctc atcaaattgg
      301 ccgaccggct tcataatatg cgcacccttc aatatatgcg gcctgaaaaa caattgaaga
      361 ttgctgccga aaccacttac ctctttgcac cattggctca tcgactaggc ctttacagca
      421 ttaagagcga aatggaagac ctcgccctca aatttaccga acccgaaata tataaagaaa
      481 tttcacgacg catggccgaa tccgaccccg tccgagccaa cttcgccgaa gaatttatca
      541 aacccatcga aaaagacctc aaagaaaaag ggttcagata tagaatcatg agacgaaaca
      601 aatcagtcta ttctatctgg accaagatga aaaaaaaaca tatccccttc gatgaagttt
      661 acgatgtatt cgccatacgc attattctgg atgtgcccat taatgacgaa agatctgcat
      721 gctggcaggc ttattccatt gttacttctc attaccgtcc gaaacccgac cgtttgagag
      781 actggatttc cattccacgc gccaatggct atcaagcatt gcatacaaca gtgatgagca
      841 aagagggcaa atgggtcgaa gtacagattc gtagcgaacg tatggacgaa attgctgaaa
      901 aaggatatgc cgcacattgg aaatataaag gacagtatta cgatgataag aacatcgaca
      961 attggctcga tcgtatacgt gatatgcttc aaaatggaga agcaggagct cttgattttc
     1021 tcgaagatgt gcaaggttac cttttttcga gcgaaatctt cgtgttcaaa ttggatggag
     1081 aacttcgtat tttgcccgcc ggcagcaccg tccttgactt tgcatatgca atcaatcccg
     1141 atgagggaaa ccattgcata ggggccaatg taaatcataa actccagaac cgtttttatg
     1201 agctaaagag tggcgaccaa atcgagattt tgacttcgaa aattcaggaa ccatcggaag
     1261 attggttagt ccacgtaaag acttcaaggg caaaa
//
LOCUS       DAORPD010000620         1294 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_21367_length_1294_cov_1.931396, whole genome shotgun sequence.
ACCESSION   DAORPD010000620 DAORPD010000000
VERSION     DAORPD010000620.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21367_length_1294_cov_1.931396"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="PK976_07325"
     CDS             complement(<1..466)
                     /locus_tag="PK976_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HRC67734.1"
                     /translation="MDENIQNEELFPEDSIKENETSASSEYRTITLRGMYENWFLDYA
                     TYVIMERAIPEMADGLKPVQRRLLHAMYEMDDGRYNKVANIIGQTMKYHPHGDQSIGD
                     ALIQLGQKELLIDCQGNWGNIFTGDQAAAPRYIEARLSKFSLEVVFNPKTTQW"
     gene            complement(499..>1294)
                     /locus_tag="PK976_07330"
     CDS             complement(499..>1294)
                     /locus_tag="PK976_07330"
                     /inference="COORDINATES: protein motif:HMM:NF012481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HRC67735.1"
                     /translation="WPETMMTYESTEKILFSADAFGSYGTLDGGIFDDELNLSFYEDE
                     MRRYYSNIVGKYGLQVQKALAKLESLDLRMIASTHGPIWRLHIPKVVEFYRNWSTYKT
                     EQGVVIAFGSMYGNTEKMADIIARAIAEEDIRNVRIYDVSKTHSSYIISDIWKYRGVI
                     LGSCAYNGGIFTPMRDLLMQLEHIMPRNHFLSIFGSMSWGGGGVSTLKKFTENIKWEL
                     IGEAIEVKHSPSDKDIVSLIELGKQMARRLKENDSSFSENNKPEMI"
BASE COUNT          335 a          300 c          245 g          414 t
ORIGIN      
        1 tccattgagt cgttttagga ttgaatacga cttcgaggga aaattttgag agacgcgctt
       61 cgatgtatcg tggtgcggca gcctgatcac ccgtgaaaat atttccccaa ttcccttgac
      121 aatcgattaa aagctctttt tgccccagtt gtatcagtgc gtcgccaata gattgatcgc
      181 catgaggatg atatttcatg gtttggccta tgatgttggc caccttatta tagcgtccgt
      241 cgtccatttc gtacatggca tggagaaggc ggcgttgtac cggtttaagg ccatcggcca
      301 tctcggggat ggctctttcc atgatcacat aggtggcgta atcgagaaac cagttctcgt
      361 acattcccct caaagtaatt gttctatact ctgagcttgc tgaggtttca ttttccttga
      421 tggaatcctc gggaaataat tcttcgtttt gaatattttc gtccatgcct tacaccccgt
      481 ccgtaaaagt aggatgggtc aaatcatttc cggtttattg ttttcactga acgacgaatc
      541 gttttctttt aatcttctgg ccatttgttt acccagttct atcaatgata caatatcttt
      601 gtcacttggg ctgtgcttaa cttcgatagc ctcgccaatc agttcccatt tgatattttc
      661 ggtaaatttt tttaaagtac ttactccacc gcctccccag ctcatcgaac caaaaataga
      721 tagaaagtga ttcctgggca taatatgttc caattgcatc aggaggtcgc gcatgggagt
      781 aaatattcca ccattatatg cacagctgcc cagaataacc cctcgatatt tccatatatc
      841 gctaatgata taactgctat gcgttttcga tacatcgtaa attctgacat tgcgaatatc
      901 ttcttcggct attgctctcg caataatatc ggccatcttt tcagtattgc catacatcga
      961 accaaatgct atcactactc cttgttcagt tttataagta ctccagtttc tataaaattc
     1021 cacaactttc ggaatatgta aacgccagat agggccatga gtcgaagcga tcatgcgcaa
     1081 gtcgagcgac tcgagtttag cgagggcttt ctgtacctgt aatccatatt tacccacaat
     1141 attcgaataa tatcttctca tttcgtcttc ataaaacgaa agattcaatt catcatcaaa
     1201 tatgcctccg tccaaggtac catagcttcc gaaagcatcg gccgaaaaaa gtattttctc
     1261 tgtcgattca taagtcatca tggtttcggg ccaa
//
LOCUS       DAORPD010000621         1293 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_21392_length_1293_cov_2.807754, whole genome shotgun sequence.
ACCESSION   DAORPD010000621 DAORPD010000000
VERSION     DAORPD010000621.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21392_length_1293_cov_2.807754"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            262..483
                     /locus_tag="PK976_07335"
     CDS             262..483
                     /locus_tag="PK976_07335"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC67736.1"
                     /translation="MYPNPVKDYQIIESGPQISEAILLYNLSGKLVLRSWLQTQTQRL
                     STSFLPSELYLWNIQYHGKTIEQGKWTKE"
     gene            719..1054
                     /gene="rbfA"
                     /locus_tag="PK976_07340"
     CDS             719..1054
                     /gene="rbfA"
                     /locus_tag="PK976_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HRC67737.1"
                     /translation="MEKTRLEKVARLIQKEMSLMLQDAVPYTGGGILTVTRVSVTPDL
                     QIARIHISIFGLPDKKAALEQLKLHGPELRYQLGVKIRHQVRIIPQLQFFLDDSLDYI
                     ERIDKLLNQ"
     gene            1060..>1293
                     /locus_tag="PK976_07345"
     CDS             1060..>1293
                     /locus_tag="PK976_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67738.1"
                     /translation="MQYDPIKSDLGRFFNRTPRLRILFYRLLNLLLLRTWHVKKELRI
                     WESQHRETAKILDAGMGFGQYSYLLAQHHPNWEI"
BASE COUNT          401 a          264 c          267 g          361 t
ORIGIN      
        1 catcaaagcc ctcatggtat tgtatcgcca tgttaaatga tacgctcgga ttggaatggc
       61 aaaggtttta tggagcagat gtatattatg gcctttggga tatcctagcg accagtgacg
      121 gtgggtgttt gctgggagga actcaatatg aatggtggaa taatgattac caccaagaaa
      181 tcgtatctat caaaaccgat tccttagggt tgattccatc atcagatgaa ggggtaagaa
      241 tactcgtccc acgatgccat agtgtatccc aatcccgtca aggattatca aatcatcgaa
      301 agcgggcctc agattagcga agccattttg ctctacaatt tgtcaggtaa gctcgtgtta
      361 cgcagctggc tacaaaccca aacacaacgg ctctccactt catttctacc ttctgaattg
      421 tatttatgga atatccaata tcacggcaaa accatagagc aggggaaatg gacgaaggag
      481 taatggttgg agccttcgaa taagataatt ctcactacag tattcgcagg tgaattttcc
      541 tgaagctaaa tgctccgcta atctagccat atttctaact attcccatat tttcaatgat
      601 agaagatgca aagccaaaaa tttgtagtat tttttcgata attttgcaaa aattccgaaa
      661 atcgtcagat gattaaaaat gatctaagcc aaagcttatt gaacagggaa gttagaaaat
      721 ggaaaagaca agattggaaa aggtagccag gctcatacaa aaggaaatga gtcttatgct
      781 gcaggatgct gtcccttata caggaggagg catacttacg gtaacccgtg taagtgttac
      841 acccgatttg cagattgcac gtatccacat tagtatattt ggtctgcccg ataagaaagc
      901 cgctctcgaa cagctcaaat tgcacgggcc agaattacgc tatcagcttg gagtcaaaat
      961 caggcatcag gtacgtatca tccctcaact tcaatttttt ctcgacgata gtttagatta
     1021 tattgaacgc attgataaac ttttgaacca ataaacctta tgcaatacga cccgattaaa
     1081 tcggatttgg gaaggttttt caatcgaact cctcgcttac gcattctttt ttaccgattg
     1141 ttgaacttac ttttattacg tacctggcat gtaaaaaaag agcttcgaat ttgggaatcg
     1201 caacatcgag aaacggccaa gatacttgat gcaggcatgg gcttcgggca atattcctat
     1261 cttctggctc aacatcaccc caattgggaa atc
//
LOCUS       DAORPD010000622         1293 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_21397_length_1293_cov_2.252827, whole genome shotgun sequence.
ACCESSION   DAORPD010000622 DAORPD010000000
VERSION     DAORPD010000622.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21397_length_1293_cov_2.252827"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            43..885
                     /locus_tag="PK976_07350"
     CDS             43..885
                     /locus_tag="PK976_07350"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRC67739.1"
                     /translation="MKIISIIAENWKADGGAAFGVVPKTIWGKLYPADDDNLQKITSR
                     CLLVDTGSRVILFDTGMGNKQDEKFFRYRYRFGDDSLFENLKYAGYSADDITDAVFTH
                     LHFDHCGGASCWNSEGSKVVRVFPNATYHVSKAQAQWALYPNKREAASYLKENLEPIF
                     EAGPLNLIEKPGPFIQQIDLRFYNGHTSGQIIPFIHLNNQTVVFVSDFIPSSAHLPLV
                     YIPAYDIQPLITLTEKEEFLHEAIEKQYILVFGHDYFNEAATVIIKGKGFQAEKIGKL
                     SEFI"
     gene            complement(1028..>1293)
                     /locus_tag="PK976_07355"
     CDS             complement(1028..>1293)
                     /locus_tag="PK976_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67740.1"
                     /translation="KKWEIIPVVIDTNLRAVEDSVRNNHYTWPVIADGKGWDTPPAID
                     YGINVTPAMFFIGPDLNIIAKPSDFSEVLDLALTYGLIKINSH"
BASE COUNT          395 a          248 c          261 g          389 t
ORIGIN      
        1 tggctggcca aatttaaaag attcttacaa taaactgaac caatgaagat catctcaatc
       61 attgccgaga actggaaagc cgatggagga gccgcatttg gcgttgttcc caagacaatt
      121 tgggggaaat tatatcctgc tgatgacgat aatctgcaaa agattaccag caggtgctta
      181 ctggttgaca cgggttcgag ggtaattttg ttcgatacag gcatgggaaa caaacaggac
      241 gaaaagtttt tccggtaccg ctatcgtttt ggagatgata gtttgttcga aaacctgaaa
      301 tatgcaggtt attcggccga tgacatcacc gatgcggtct ttacacattt gcacttcgat
      361 cattgtggag gagcctcatg ttggaatagc gaaggttcga aggtagtacg tgtttttccg
      421 aatgccacct atcatgtttc gaaagctcaa gcccaatggg ccttgtatcc caataaaaga
      481 gaagctgctt cgtatctgaa agaaaatctc gaacctattt tcgaagctgg ccctttgaac
      541 cttatagaaa agcccgggcc attcattcag caaattgatt tgcgtttcta taatggacat
      601 accagcggtc agataatccc tttcatccat cttaacaacc aaaccgtcgt ttttgtgagc
      661 gattttatcc catcttcggc tcatctgcct ttggtatata ttcctgctta tgatatccaa
      721 cctttgatta cccttaccga aaaggaagaa ttccttcacg aagccattga aaagcagtat
      781 attttagttt ttggtcatga ttacttcaac gaagctgcca ccgtaatcat caaaggaaag
      841 ggttttcagg ccgagaaaat aggtaaacta tccgaattta tttgaaaatt cagttgaaaa
      901 taatttatct tattgaattt taaagtattg ctgaataaac ttttgaaaga aatagtaaaa
      961 aacaaaaaag tggtataaat cattcatata caatatttta caaacaaact ttcaaaactg
     1021 taatttgtta gtgcgaattt atttttatta gcccgtaggt aagagcaagg tcaagtactt
     1081 ctgaaaagtc agagggctta gcgatgatat tgaggtcggg gccgataaaa aacatggcag
     1141 gtgtaacatt aattccatag tcgattgccg gcggtgtatc ccatcctttt ccatctgcaa
     1201 ttacaggcca tgtgtaatga ttatttcgta ccgaatcttc aacggctcgt aaattagtat
     1261 cgatcacaac ggggattatt tcccactttt ttt
//
LOCUS       DAORPD010000623         1293 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_21410_length_1293_cov_1.411955, whole genome shotgun sequence.
ACCESSION   DAORPD010000623 DAORPD010000000
VERSION     DAORPD010000623.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21410_length_1293_cov_1.411955"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(278..>1293)
                     /locus_tag="PK976_07360"
     CDS             complement(278..>1293)
                     /locus_tag="PK976_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HRC67741.1"
                     /translation="ALRRLGFDSVLDTDFTADLTIMEEGTELLTRLKKVLVDKDTSMA
                     LPMFTSCSPGWIKFIEHTFPDLLPHLSTCKSPQQMFGALAKTYYAQKKGINPKDIVSV
                     SIMPCTAKKFEATRPEMRDSGYQDVDYVLTTRELAIMIKQAGIEFMKLDDEDYDRFMG
                     ESSGAGVIFGATGGVMEAALRTAYEIVTGREVPFQNLNITPVRGMEGIKEAEVKIENV
                     KPDWSFLEGVTLRVAIAHGLANAKRLMTAIRDGKVVYHFIEIMACPGGCLGGGGQPIP
                     TNLEIRKKRAEAIYAEDENLPIRKSHENPEIIQIYQEFLGEPLSHTSHELLHTHYVPR
                     KRY"
BASE COUNT          370 a          299 c          231 g          393 t
ORIGIN      
        1 tcatcatcac gtcgagtatg gccagatcgg gtttctcggc tcttgctctt tcaaagcctg
       61 caaacttatc acctgccgta atcacctgaa aaccttcttt ctctaaaaaa gctttctgga
      121 taaatacaaa atctatatca tcatctacca acaatatttt aatgtctttc ataatgactg
      181 aaattttaaa tgtttcgact tttgttgaat tcttattttc tcgataaaat taagaaattt
      241 cacgatactt cattactaaa tccagttatt gtctaaatta ataacgtttt cgaggaacat
      301 aatgcgtatg cagtaattca tgcgatgtat ggctcaaggg ttcacccaaa aattcctgat
      361 agatttgaat aatttcagga ttttcgtggg atttacgaat aggcaggttt tcatcttctg
      421 cataaattgc ctctgcacgt ttctttctga tttccagatt ggttggtatc ggttggccac
      481 caccacccag gcatcctcca ggacatgcca tgatttcgat aaaatgataa acaaccttgc
      541 catcacgtat agcagtcatc agcctcttgg cattggccaa accatgagca atagcaaccc
      601 tcaacgtaac tccttcgagg aaactccagt caggttttac attttcgatt ttgacctcgg
      661 cttctttaat gccttccatg ccccttacag gggtaatatt caaattttga aatgggactt
      721 cacgacctgt gacaatttcg taagccgtac gcaaagctgc ctccatcact ccaccagttg
      781 ctccaaaaat aacaccagct cccgatgatt cacccataaa acgatcataa tcttcgtcat
      841 cgagcttcat aaattctatg ccggcttgtt ttatcataat agccagctcg cgggtggtta
      901 agacataatc tacatcctga tagccactat cgcgcatttc gggcctcgta gcctcgaatt
      961 tcttggctgt acagggcata atggaaactg atacaatgtc cttgggatta atccctttct
     1021 tttgagcata gtaagtttta gccaatgcgc caaacatctg ctggggagat ttgcaggttg
     1081 aaagatgagg cagaaggtcg gggaaagtat gttctataaa cttgatccat ccaggcgaac
     1141 aggaagtaaa cataggtaga gccatcgatg tatccttatc aaccaatact tttttcaaac
     1201 gagtaagcag ttctgttcct tcttccataa tcgttaaatc agccgtaaaa tcggtatcca
     1261 acaccgaatc gaatcccagt ctacgaagag ctg
//
LOCUS       DAORPD010000624         1290 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_21459_length_1290_cov_2.404049, whole genome shotgun sequence.
ACCESSION   DAORPD010000624 DAORPD010000000
VERSION     DAORPD010000624.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1290
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21459_length_1290_cov_2.404049"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="PK976_07365"
     CDS             complement(<1..219)
                     /locus_tag="PK976_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HRC67742.1"
                     /translation="MQNGKKIFFNSDQHFGAPTAKESKVRERIFIEWLEHIAPEAEEI
                     YLMGDLFDFWFDYKAVVPKGYTLLLGALA"
     gene            complement(382..>1290)
                     /locus_tag="PK976_07370"
     CDS             complement(382..>1290)
                     /locus_tag="PK976_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018360874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HRC67743.1"
                     /translation="ARLNLTYTIMSKRKLLELVKEGYVKGWDDPRMPTICGLRRRGYT
                     PASIRNFAEKIGVARRDNIIDVSLLEFSLREDLNKTAQRRLAVLNPLHLIIENFPQDE
                     TELLPAANNPEDAGAGIRKIPFSRELWIERDDFMIDPPKKYYRLYPGSEVRLKYGYII
                     KCQRYETDPESGEVKTVYCTYDPESKTGGATANRKIKGTIHWVSKKHALKAEIRLYDR
                     LFIKPDPDDVAEGESFINYLNPDSLKIMNAWCEPALMDATTDKHYQFERIGYFYLDPD
                     SCPGYLVFNRTVSLKDSWSKMSQNEE"
BASE COUNT          344 a          248 c          279 g          419 t
ORIGIN      
        1 ggcaagagct cctaataaaa gtgtgtaacc ctttggaacg actgctttat aatcgaacca
       61 gaaatcgaac aaatcgccca tcaaataaat ttcttcggct tcgggtgcaa tgtgctcgag
      121 ccattcgata aaaattcttt ccctcacttt actttctttg gcagtgggtg caccaaaatg
      181 ctggtcggag ttgaaaaata tttttttacc gttttgcatc ttcatgcaaa tttatcaaga
      241 aatgttttgg ccattattga ttttggccga gtcatctcgg gctggctgaa aaataatcgg
      301 attattatgt gaagatgtta gtgcaaaaaa aaataatgat aaggaagatt tgttaatctt
      361 gttttgtttt atgcaatgtg attattcttc gttctgactc attttgctcc atgaatcttt
      421 caaagatacg gttctgttaa acacgaggta tcctggacat gaatcaggat cgagatagaa
      481 atatccaatt ctttcgaact gataatgttt gtctgtggtg gcatccatta atgcgggttc
      541 gcaccaggca ttcattattt tgagtgaatc ggggttcaga taattgataa aactttcgcc
      601 ttcagctaca tcgtcgggat cgggttttat gaaaagcctg tcataaagtc tgatttcggc
      661 ctttagcgca tgttttttcg atacccaatg aatagttccc ttgatttttc ggttggctgt
      721 tgctccaccg gttttcgatt cagggtcata agtgcaatat acggttttta cctcccctga
      781 ttcaggatca gtttcgtaac gctgacattt gataatatat ccgtatttta gtctaacttc
      841 cgaaccggga taaagacggt aatatttttt gggaggatcg atcatgaagt cgtctctttc
      901 gatccacaac tcgcgtgaaa atgggatttt gcgaatgcct gctcctgcgt cttctggatt
      961 attggcagcc ggaagaagtt cggtttcatc ttggggaaaa ttttcaatga taagatgtag
     1021 aggattcaat acagcgagtc ttcgttgagc ggttttgttc aagtcttcac gcaagctaaa
     1081 ttccaatagc gaaacatcga tgatattgtc tcggcgggcc acaccgattt tttcggcaaa
     1141 attgcgaata gatgctgggg tataaccgcg gcggcgtaaa ccacaaatgg taggcatgcg
     1201 tggatcatcc catcctttta cataaccttc ttttacaagc tccaaaagct tacgtttact
     1261 catgatagta taagtaaggt tcaggcgggc
//
LOCUS       DAORPD010000625         1290 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_21465_length_1290_cov_1.923887, whole genome shotgun sequence.
ACCESSION   DAORPD010000625 DAORPD010000000
VERSION     DAORPD010000625.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1290
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21465_length_1290_cov_1.923887"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1290)
                     /locus_tag="PK976_07375"
     CDS             complement(<1..>1290)
                     /locus_tag="PK976_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67744.1"
                     /translation="QVFVNELKANWQSHYELDEMIPQVNSLVEKMLERRLKPYEHIAP
                     FLTTIVNLAILNAEQGQVKPFLDGVSYLLDQPGPSQLNDFYHISNSLLRDSLLSRYKN
                     INWKVSQIRFEIINYPTPRFKFKDIDLICQMGSSRRKIYQTLGIYDPFSREWKGVGGQ
                     TPWVNNNIPSDSVYVEFDRYGIFLQGTDIQADSAMLYHKTYFPSGVMGKFEDNFKPGN
                     KPGLPRFVSYDRNIKIPGISQNVDFEGGVKLETHQLNGIGESGRPARLFFYTPQKNRV
                     VIKSQQFFFKNPFISALDANATIRYRFDSIFHPAISFVYDENKRQLNLYQGNSILSSL
                     PFFSSYQRMEIQANTLLWNIDDSLMIFKKGAGLVRENDAVFISENYFRGEDFRTLQGI
                     DPVNPLIRLYQLAKQLHKSQFGLNEYASAIHLSADQAE"
BASE COUNT          398 a          244 c          267 g          381 t
ORIGIN      
        1 ctcggcttga tcggccgaaa gatgaatggc acttgcatac tcattcaatc cgaattgtga
       61 tttatgaagt tgtttagcaa gttgatacag acgtatgaga gggtttacgg ggtctatgcc
      121 ctgaagggtt ctaaaatctt ctcccctgaa ataattttct gagataaaaa cggcatcatt
      181 ttctcgcaca agtccggctc cttttttaaa aatcatgagc gaatcatcga tattccataa
      241 aagtgtatta gcctgaattt ccattctttg ataggagcta aaaaagggca aagatgatag
      301 aatgctattg ccttgataaa gattcaattg tcgtttgttc tcgtcataaa caaatgaaat
      361 ggcgggatga aagatagaat cgaaccgata tcgaatagtc gcattggcgt cgagagccga
      421 tataaaagga tttttgaaaa agaattgctg agattttatg acgactctat ttttctgtgg
      481 agtataaaaa aaaagccggg ctggtcttcc ggattcacca attccgttaa gctggtgggt
      541 ctctaatttt accccccctt caaaatcgac attctgagaa attccgggga ttttaatatt
      601 ccgatcataa gatacaaaac gcggcaaacc aggtttatta ccaggtttga aattatcttc
      661 gaatttcccc attactcccg aagggaaata ggttttatga tacaacatcg ccgaatcggc
      721 ctggatatca gtcccttgta gaaatattcc atatcggtcg aactcaacat aaacagaatc
      781 agatggtata ttgttattta cccagggagt ttgtccaccc acacctttcc attcgcgtga
      841 aaagggatca taaatgccta aagtctggta aattttacgc cgtgaagaac ccatctggca
      901 aatcaggtcg atgtctttaa atttgaaacg aggtgttgga taattaatga tttcgaatcg
      961 tatttgactg actttccaat tgatgttctt atagcggcta agcaagctat ctcgaagcaa
     1021 ggaattggaa atgtgataaa aatcgttcaa ttgagaagga ccgggctgat caagaagata
     1081 agaaactcca tcaaggaatg gttttacctg cccctgctca gcattgagaa tggcaagatt
     1141 tacgatagtc gtaagaaaag gagcaatatg ttcataaggc ttcaagcgtc gttccaacat
     1201 cttttcgacc agagaattca cttgtgggat catttcatcg agttcgtaat gtgattgcca
     1261 atttgccttt aactcgttta caaatacctg
//
LOCUS       DAORPD010000626         1290 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_21468_length_1290_cov_1.896356, whole genome shotgun sequence.
ACCESSION   DAORPD010000626 DAORPD010000000
VERSION     DAORPD010000626.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1290
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21468_length_1290_cov_1.896356"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1173)
                     /locus_tag="PK976_07380"
     CDS             complement(<1..1173)
                     /locus_tag="PK976_07380"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRC67745.1"
                     /translation="MQLHKLRNIGIAAHIDAGKTTVTERILYFTGANRKMGEVHNGEA
                     TTDFMKQEQERGITIASAAITCKWKEHHINLIDTPGHVDFTVEVERSLRVLDGMIAVF
                     CAVGGVEPQSETVWNQANRYKVPRIAFINKMDRQGADFENVVAQMNKYLDANPIPFQI
                     PIGNEEDFKGIIDLIKMKALFFENFESTEAEIPAEYKGSASRARQYMIEKLADFNEEI
                     LEKYLNEEPIPEELIIEASRYTTLHLLATPVLCGAAYKNKGITQLLDAVIHFLPSPLD
                     VGAVVGMDRTDPEKTHSRYPSPKDPFSALAFKIIYDHYVGQQTFIRIFSGRLSTGMQV
                     LNSSKDKNERIGRIMRIHAKMREDIPEAGPGDIVALVGLKFTKTGDTLCDPDNPLLL"
BASE COUNT          359 a          278 c          245 g          408 t
ORIGIN      
        1 gagcaacaaa ggattgtcag ggtcgcagag ggtatcgccg gttttagtaa acttaagccc
       61 gaccaatgca acaatatcac ccggcccagc ttcaggaatg tcttctcgca tcttggcatg
      121 aattcgcata atccgtccta ttcgctcgtt tttatctttt gaactattta gcacctgcat
      181 gcctgtactc aatctcccac taaaaattct gataaaagtc tgctgcccta cataatgatc
      241 gtaaataatc ttgaaggcaa gtgctgagaa aggatcttta ggagaagggt agcgcgagtg
      301 tgttttttcg ggatctgttc gatccatacc cacaacagca cctacatcga gaggactggg
      361 caagaaatga atgactgcat ccagcaattg tgtaatcccc ttgtttttat aggcagctcc
      421 acaaagtacg ggtgtagcta agaggtgcaa agttgtgtaa cgactagcct ctataattaa
      481 ttcttcaggg atgggttcct cgttgagata tttctccaaa atttcttcat tgaaatcggc
      541 gagtttttct atcatatatt gtctggctcg gctggccgat cccttgtatt cagcaggtat
      601 ttcggcttct gtgctttcga aattctcaaa aaaaagagcc ttcatcttta ttaaatcgat
      661 gattccctta aaatcttctt cattcccaat aggaatctgg aaaggaatag ggttggcatc
      721 aaggtattta ttcatttgtg ccactacatt ttcgaaatcg gctccctgcc tgtccatttt
      781 attgatgaaa gcgattcgtg gtactttata gcggttagct tgattccaaa ctgtttcact
      841 ttgtggctct accccaccta cggcgcaaaa aaccgcaatc ataccatcca aaacacgcag
      901 agatcgttca acttcaaccg taaaatcgac atgtccgggt gtatctatca gattgatatg
      961 atgttctttc catttacagg tgatggctgc cgaagctatg gttatccctc tttcctgttc
     1021 ctgcttcatg aaatcggtag tggcctctcc attgtgcacc tcgcccatct ttcgatttgc
     1081 acctgtaaaa tataaaattc tttcggtaac tgtggttttt cctgcatcta tatgggctgc
     1141 aatgccaatg tttcttaatt tatgtaattg cataaaaatg attaatcttg aaataattaa
     1201 ttaaaataca atattttata ttttaattcg ataaaagggg gcaaatctac aaacattcta
     1261 tcagaaaatg gaaaatcagc aaaaattctc
//
LOCUS       DAORPD010000627         1289 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_21495_length_1289_cov_2.316045, whole genome shotgun sequence.
ACCESSION   DAORPD010000627 DAORPD010000000
VERSION     DAORPD010000627.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21495_length_1289_cov_2.316045"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..>1289)
                     /locus_tag="PK976_07385"
     CDS             complement(113..>1289)
                     /locus_tag="PK976_07385"
                     /inference="COORDINATES: protein motif:HMM:NF015588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HRC67746.1"
                     /translation="GREETQVVHLNPVSGEWGDDYSSIQRHVSLAIAYNIWEYYHSTL
                     DQQFLNDYGAELFLDICMFWTGLCHKDAEDGRYHIRGVMGPDEFHEKYPNAKRGGLDD
                     NAYSNLMTVWALQKAYQMRNIIGEECFRNVARKIGLNEQEWQRWKNIRQNLALCINEE
                     GIIAQFDGYFELKELDWEYFKKKYGNVYRMDRLLKAEGLSPDEFKVAKQADVLMIFYN
                     LLPEEVNRLLQEMGYDLPKDYLKKNFWYYLNRTSHGSTLSRVVHAKLAHLVGEEKLAW
                     QLFREALISDYIDIQGGTTAEGIHASVMAGTVWLALTVYAGLSLCEDIPSFQPDLPYD
                     WEAMKFNFIFRGIRYYVEIDRQKIVLTAGAPHNIEVKAKVWGKEYILLPGIAKTIEI"
BASE COUNT          371 a          290 c          215 g          413 t
ORIGIN      
        1 aatacctttt caaggatcgt tttagctgcc tttttatgct ttttgttgat gatgagaaca
       61 ttctcgtaca ttttccttca attttccgtt tcgaaacgta caacaaaact aattagattt
      121 cgattgtttt tgcaatgcca ggcaacaaaa tatattcctt tccccacact ttcgccttga
      181 cttcgatatt atgtggtgca ccagctgtaa gaactatttt ttgcctatct atttctacat
      241 agtatctgat gcctcgaaaa ataaaattaa atttcattgc ttcccaatcg tatggtaaat
      301 caggttgaaa cgaagggata tcttcgcaca ggctcagtcc agcataaaca gttaaggcaa
      361 gccagacggt tccggccatt acactcgcat gaattccttc ggctgtagtc ccgccctgta
      421 tatcgatata atcactgatt aaagcctcac gaaacagttg ccatgccaat ttctcctcac
      481 ctaccaaatg agccagtttg gcatgaacta ctctgctaag cgtgctgcca tgtgaggtac
      541 gatttaaata ataccaaaaa tttttcttta aataatcttt gggtaaatca taacccattt
      601 cttgaagcaa acggttcact tcttcgggca aaagattata gaatatcatc aaaacatcgg
      661 cctgcttggc caccttgaat tcatcgggcg ataagccttc ggcttttaat aaacgatcca
      721 tgcgataaac attgccatat ttcttcttaa aatattccca atcgagttcc ttgagttcga
      781 aatatccatc gaattgagcg ataataccct cttcattaat acaaagtgca agattttgcc
      841 ggatattttt ccatctttgc cattcttgtt cgtttaaacc gatttttctg gcaacgtttc
      901 gaaaacattc ttcaccaatg atatttctca tttgataggc cttttgtaga gcccagactg
      961 tcattaagtt ggaataagca ttatcgtcga ggcctccacg cttagcatta ggatatttct
     1021 catgaaactc atctggtccc attacaccgc gaatgtggta tcttccatct tcggcatcct
     1081 tatggcataa gccagtccag aacatgcaga tatctaaaaa taattcggct ccataatcat
     1141 tcagaaattg ttgatcgaga gtagaatgat aatattccca tatattataa gctatagcca
     1201 gtgaaacatg gcgctgaatg gagctgtaat cgtcgcccca ttcaccagaa acaggattga
     1261 gatgtactac ctgagtttct tctctacct
//
LOCUS       DAORPD010000628         1286 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_21566_length_1286_cov_2.548335, whole genome shotgun sequence.
ACCESSION   DAORPD010000628 DAORPD010000000
VERSION     DAORPD010000628.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21566_length_1286_cov_2.548335"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(301..>1286)
                     /locus_tag="PK976_07390"
     CDS             complement(301..>1286)
                     /locus_tag="PK976_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005778254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRC67747.1"
                     /translation="GMYGGDRSRKQVLVDYGFRLPSALDNRPLKFDEFEKLTNQIIYV
                     SATPAEYELKQAEGIVVEQFIRPTGLLDPKIEVRPSTNQIDDLLSEIQKNVERNERVL
                     VTTLTKRMAEELSKYLQSINVKTRYIHSDIETLNRVEIMRDLRLGKFDVLVGVNLLRE
                     GLDLPEVSLVAILDADKEGFLRSERSLTQTAGRAARNINSRVIMYADKITDSMRLTIE
                     ETNRRRSMQTEYNQMHGITPTPIIKSTSEILGQTSVVDVKKPQSNYYVEKEEIEVAAD
                     PLVKYLNKEQIQKIIQQTRKNMEAAVKELDFINAARFRDEINQLKQLLETK"
BASE COUNT          330 a          270 c          250 g          436 t
ORIGIN      
        1 gattttaacc atcctctggc cggaatgacc ttatttttta cgggttccat tctcgatgta
       61 agaccagcct ctcccgaaga actggcccat ggacatgttc attgagaagg caaccacggc
      121 tgttgattcc ataataattc atctgaatta aattttttcg gactacctga agattcaacc
      181 cccttgattt tatatttggc aaattttcaa tagttggagc taattttata acttactatt
      241 atacaaaata ttacaaaata gaatcaacaa tatttgttga taagaactta taaaaatgtg
      301 ttatttagtt tcgagaagtt gtttaagctg gttaatttcg tctcgaaaac gggctgcgtt
      361 gataaaatcg agttctttta cggctgcttc catatttttt cgggtttgct gaatgatttt
      421 ttgtatctgc tctttgttta gatatttcac aagagggtcg gcagcgactt caatctcttc
      481 tttttcaaca taataattgg actgaggctt tttcacatct accaccgagg tttgcccaag
      541 aatttcactc gtcgatttga tgatgggggt aggagtgatg ccgtgcatct ggttgtattc
      601 cgtttgcatc gatcgccttc tatttgtttc ctcgatagtc aaccgcatag aatcggtaat
      661 cttatcagca tacataatga cccggctgtt gatatttcgg gcggcacgtc cggctgtttg
      721 cgttaacgat cgttccgagc gcaagaaacc ttccttgtca gcatccagga tagctaccaa
      781 tgaaacttca ggcaggtcga gcccttctct tagaagattt actcccacca atacatcgaa
      841 tttcccaagt cgtaaatcac gcatgatttc gaccctgttg agggtctcga tatcagaatg
      901 aatgtagcga gttttaacat tgatactttg caaatattta ctgagttcct ctgccattct
      961 tttagttaag gttgtaacca gaactctttc atttctttcc acattttttt gaatttccga
     1021 caataagtca tcgatctggt tggttgaagg tctgacttct attttagggt cgagaagtcc
     1081 ggttggtctg atgaattgtt ccactactat tccttcggcc tgttttaatt catactccgc
     1141 tggagtagca ctcacataaa ttatttgatt ggttagtttt tcgaactcat cgaatttcag
     1201 aggtcggtta tcgagggcag aagggagccg aaacccataa tctaccagaa cctgcttgcg
     1261 tgaacgatca cctccataca ttccac
//
LOCUS       DAORPD010000629         1284 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_21648_length_1284_cov_2.301871, whole genome shotgun sequence.
ACCESSION   DAORPD010000629 DAORPD010000000
VERSION     DAORPD010000629.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21648_length_1284_cov_2.301871"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="PK976_07395"
     CDS             <1..366
                     /locus_tag="PK976_07395"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRC67748.1"
                     /translation="DKGAAAIGTTRCPNAQAFHIYFLKTDSLGLVTGEKEISLPKTEE
                     AELSIYPNPVRESCHIHFGLGARQIQLFNLQGQVTREWLVPQGCTFMDVSLKNLPTGI
                     YLFRVTGNEGVKSMRVVKM"
     gene            720..>1284
                     /locus_tag="PK976_07400"
     CDS             720..>1284
                     /locus_tag="PK976_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008617076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRC67749.1"
                     /translation="MSDEEKMQIAAETYTADNIQVLEGLEAVRKRPAMYIGDVDVRGL
                     HHLVYEVIDNSIDEAMAGYCNDIKVIIHPDNSITVRDNGRGIPVDMHEKEKRSALEVV
                     MTVLHAGGKFDKDSYKVSGGLHGVGVSCVNALSSNLWVKIYREGKIWYQEYERGKPIE
                     PVKIIGESTMTGTETHFKPDPNIFNAIV"
BASE COUNT          432 a          222 c          288 g          342 t
ORIGIN      
        1 gataaaggag ccgctgctat aggaactacc cgctgcccaa atgcacaagc atttcatatc
       61 tattttttaa aaaccgatag cctgggtttg gttacggggg aaaaagaaat ttcccttcct
      121 aaaaccgaag aagcagagct cagtatatat cccaatccag ttagagaaag ttgccatatt
      181 cattttggcc ttggagcaag gcaaattcaa ctattcaacc ttcagggcca ggtaacaaga
      241 gaatggcttg tgccccaagg ttgtaccttc atggatgtat cgttgaaaaa tcttccaaca
      301 gggatttatc tattcagagt aactggaaat gagggagtga aaagcatgag agtagtaaaa
      361 atgtagataa gcttaaacac gcggatagga taaattggta actattggaa acatgccaaa
      421 atatgggtaa tttttttaat ttcaatggct gggataagct ggtattttct ttcccacaaa
      481 aacaaattta agaattaagc ctttgcttgc tgccctgcag gtctaaaata tttcctcttt
      541 tcatgataga aacttataaa catacttaga acaaaatcgg gcagatagcc gattcattga
      601 aataaaattt accgaacagc cttatctttt gaaaagattt attcataaat tttcgtcctg
      661 ggtaagagaa aaaatagtat ctttgcaaac gtaaatgatt gaaaaataat tatttgatta
      721 tgagtgacga agaaaaaatg cagatagcag ccgagaccta tacggccgac aacattcagg
      781 tattggaagg gttggaagcg gtacgcaaaa ggccggcgat gtacattggc gatgttgatg
      841 taagaggatt gcatcatctg gtttacgaag tgattgacaa ttcgatagac gaagccatgg
      901 ctggctactg caacgatatt aaggtgatca ttcacccaga caattccatc accgttaggg
      961 ataatggcag aggaatcccc gtagatatgc atgaaaaaga aaagcgttcg gcacttgaag
     1021 tggtcatgac ggttttacat gcgggaggga aattcgacaa ggattcgtat aaagtatcgg
     1081 gcggcttgca tggcgtgggg gtatcgtgtg taaatgctct ttcatcgaat ctttgggtaa
     1141 aaatatatag agaaggtaaa atctggtatc aggaatatga aagaggcaaa cccatagaac
     1201 ctgtcaaaat tataggagaa agtaccatga cagggaccga aacgcatttc aagcccgatc
     1261 ccaatatttt taatgccatt gtct
//
LOCUS       DAORPD010000630         1282 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_21712_length_1282_cov_2.145069, whole genome shotgun sequence.
ACCESSION   DAORPD010000630 DAORPD010000000
VERSION     DAORPD010000630.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21712_length_1282_cov_2.145069"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="PK976_07405"
     CDS             <1..247
                     /locus_tag="PK976_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67750.1"
                     /translation="RENDNLMQTIDNIFYKPRKYNTIELWKDVKEEQWNDVNWQRRNS
                     IRSVAQLKKIIKLNEVFVKISKNSFLRCKKNLILWNH"
     gene            235..>1282
                     /locus_tag="PK976_07410"
     CDS             235..>1282
                     /locus_tag="PK976_07410"
                     /inference="COORDINATES: protein motif:HMM:NF012447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HRC67751.1"
                     /translation="MESLIVNGEGLTIEDIVAVARHHQKVVLHPEAIEKIVQCRAMLE
                     KKIEAREIMYGVNTGIGEFSEVVLTDDQVKDFQKYLIYNHAAGIGEPMPEEWVRGAML
                     GRINVHAHGNSGSRPEITLTMVEMLNKGVTPYVCMKGSVGACGDLAPMAQIALLLMGE
                     GKAYYGGELLDGKEAMRRAGIEIPGLKARDGLAAINGSNVLTAMSAIFLYDANRWLKQ
                     AEIATAMSLEALKANLKPYHELIHQVRGFPGAMRSARAIRKMVEGGDLAEGRLKCKVQ
                     DAYSMRSTPQVIGAAHDALAYARSQVEIELNGVGDNPIFFPELNLQLSGANFQGSPVS
                     LPMDMAGAAITMVCV"
BASE COUNT          389 a          234 c          333 g          326 t
ORIGIN      
        1 cagagaaaat gataatctta tgcagacaat agacaacatt ttttacaaac ctagaaaata
       61 caacacaatc gaattatgga aggatgttaa agaagagcaa tggaatgacg ttaattggca
      121 gagaagaaac tcaatacgaa gtgttgcaca gttaaaaaaa attataaaac ttaatgaagt
      181 atttgtaaag atttcgaaaa attcgtttct tcgctgtaag aaaaacctaa ttctatggaa
      241 tcattaattg taaatggcga ggggttaact atagaagata tagtagctgt agctcgacat
      301 caccagaagg tagttttgca tcccgaagca attgagaaga tcgtgcaatg ccgcgccatg
      361 ctcgaaaaaa agatcgaagc tcgcgaaatc atgtatggag tgaatactgg aattggcgag
      421 ttttctgaag tggtactgac cgatgatcag gtaaaggatt ttcaaaaata tttgatttac
      481 aatcatgctg cggggatagg cgagcccatg cccgaggaat gggtgagagg tgccatgttg
      541 ggccgtatta acgttcatgc acatggcaat tcgggaagtc gtcctgaaat cactctcaca
      601 atggtggaga tgctcaacaa gggtgttact ccttatgtct gtatgaaagg ttcggtaggt
      661 gcctgtggcg atctggcacc tatggctcaa atcgctcttt tgctgatggg tgagggcaaa
      721 gcctattatg gaggggagtt attagacggg aaggaggcca tgcgaagggc aggcatagaa
      781 atacctggtt taaaggcaag ggacgggctg gcggctataa atgggagcaa tgtacttacg
      841 gccatgagtg caatcttttt atatgatgcc aatcgctggc tgaagcaggc cgaaattgcc
      901 actgccatgt cgcttgaagc gctaaaagcc aacctgaaac cttatcatga attgatacat
      961 caggtaagag gtttcccggg tgccatgaga agtgccagag ccattcgaaa aatggtcgaa
     1021 ggaggcgatc tggccgaagg ccgattaaaa tgtaaggtac aggatgctta ttcgatgcgt
     1081 tcgacgccac aagtgattgg agcggctcat gatgcacttg cttatgcacg ttcacaagtc
     1141 gaaatcgaat tgaatggtgt aggtgataat cccattttct ttcccgaact caatcttcag
     1201 ctttcgggag ctaactttca gggcagtccg gtttcattac ccatggatat ggcaggtgca
     1261 gccatcacaa tggtttgtgt gc
//
LOCUS       DAORPD010000631         1282 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_21714_length_1282_cov_2.088020, whole genome shotgun sequence.
ACCESSION   DAORPD010000631 DAORPD010000000
VERSION     DAORPD010000631.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21714_length_1282_cov_2.088020"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..>1282
                     /locus_tag="PK976_07415"
     CDS             102..>1282
                     /locus_tag="PK976_07415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HRC67752.1"
                     /translation="MEFIYPWYLVGLAAISIPIVIHLFNFHRYKKVYFTNVRFLQAIQ
                     SETHRSSRLRNLILLLLRILFIITLVLAFAQPYIPASNNIKQGNPFAVSIYLDNSQSM
                     ETGMGNENLLHQAREKALEIVNSFSSTDKFQVLTNDLEGKYQHFVPANEASIWINDVK
                     SSLAQVSTSTLLQTQANLLRYEKNYNHLAFFISDFARSTFSLPTNLDTNITWIFIPMQ
                     SPLPLNVSIDTCWFDTPIIAPGLESVLRVNVTNHSANQSRELMVKLSTNEIQRGLKPL
                     SLTPKTTFTLSFPLFNADTGTIMGSLQINDNGFTFDDELYFAYTVHPKLEILIVNNSE
                     GENPHLNQLFHSDSLMWVDNQPIHQLIYSRLEKSSLTILNELTEYPQGLILSVRHSLN
                     AG"
BASE COUNT          363 a          268 c          228 g          423 t
ORIGIN      
        1 cctcaaggcc ttgcaaatgg gagcaaattt gattcaatat gtgtttactc aggtaattca
       61 atgaattttt acgtttcaat tgattttgtt tatattcaat catggaattt atctatcctt
      121 ggtatttagt cggattggcc gctatttcaa tccctatcgt tattcatctt ttcaattttc
      181 atcggtataa aaaagtatat tttaccaatg taaggttttt acaggctatc caatcagaga
      241 cgcatagatc gtcgcgtctt cgaaatttga ttttgttgct cttacgaata cttttcatca
      301 ttaccttggt gttagcattt gcccagcctt atattccggc ttccaacaat ataaagcagg
      361 ggaatccctt tgccgtgagc atttatttag ataactcaca gagcatggaa acaggtatgg
      421 gtaatgaaaa tctgttgcac caggccagag aaaaggccct cgaaatcgtg aacagttttt
      481 cttcgaccga caagtttcaa gtgcttacca acgatttgga agggaaatat cagcattttg
      541 tacctgccaa tgaggcgagt atctggataa atgatgttaa atcgagtctg gcacaggtaa
      601 gcacttctac tttattgcaa actcaggcta acttgcttcg ctatgaaaaa aactacaatc
      661 atttggcttt ttttatttcg gattttgcca gaagcacttt tagtttacca actaatcttg
      721 atacgaatat tacttggatt tttatcccca tgcaatcgcc tcttccctta aatgtcagta
      781 ttgatacttg ctggttcgat acaccaatta ttgctcccgg tctcgaatcc gtgcttcgtg
      841 tcaatgttac gaatcatagt gccaaccaaa gccgtgagct gatggtaaaa ctcagcacaa
      901 atgaaataca acgcggactc aaacccttaa gcttgactcc caaaactact ttcactcttt
      961 cattcccgct gtttaatgcc gatactggta ctataatggg atcgctacaa attaacgata
     1021 atggttttac tttcgacgat gagctttatt ttgcctatac tgttcatcca aagctcgaaa
     1081 tccttattgt caataatagt gaaggagaaa atcctcatct caatcagtta tttcattccg
     1141 acagtttgat gtgggttgat aatcagccta ttcatcagct tatttattcc cggctcgaaa
     1201 aatcctctct aaccattttg aacgagttga ccgaatatcc tcagggtctg atattgagcg
     1261 taagacacag tctcaatgca gg
//
LOCUS       DAORPD010000632         1279 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_21811_length_1279_cov_2.250000, whole genome shotgun sequence.
ACCESSION   DAORPD010000632 DAORPD010000000
VERSION     DAORPD010000632.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21811_length_1279_cov_2.250000"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="PK976_07420"
     CDS             complement(<1..262)
                     /locus_tag="PK976_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HRC67753.1"
                     /translation="MSEPIKHECGIAFIRLLKPLEYYLGKYGTSLYGLNKLQLLMEKQ
                     HNRGQDGAGVACIKLGLLPGKKYINRVRSNATTSIKDVFDQIF"
     gene            573..935
                     /locus_tag="PK976_07425"
     CDS             573..935
                     /locus_tag="PK976_07425"
                     /inference="COORDINATES: protein motif:HMM:NF039385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6232 family protein"
                     /protein_id="HRC67754.1"
                     /translation="MAEEKIYFDEGNVKVTNARFLTYGKMHSMANVTSVAKYVIKPKR
                     TGPIVLGIIGLICFAFKWWLAVLLLVGAIAWWMTQKNKYLVSLASASGSEEALTSADE
                     AFIDRVVSALNEAIAERG"
BASE COUNT          407 a          205 c          258 g          409 t
ORIGIN      
        1 caaaaatctg gtcgaagaca tcctttatgg atgtagttgc attggaacga actctgttga
       61 tatatttttt accaggtagt aaaccgagtt ttatacaagc cacacccgct ccatcctgtc
      121 cgcggttgtg ctgcttttcc attaacaatt gaagtttatt taaaccatag agagaagtac
      181 catatttccc aagataatat tctaagggtt taaggagtcg aataaaggca atgccgcatt
      241 catgcttgat cggttcgctc atgatagagg atacattaca caaaatcaag ctgcaaaatt
      301 acgtatttta acccctcaat aattcccaat gatagaagaa tatcaaatgg tcacaatcat
      361 cgaaggaatg ttccttccga agaaaaatga gatttcatga aaatttttca taagaaattt
      421 ggcctgctat aactgcttca taagattttt gtgaaatttt aataataaat tcttgtatta
      481 tcagaaaaac tttacctttg tatccgataa atctcaaatt ttttgaggca gtatcaaaaa
      541 caccattatt cacattcaaa aataaaattt ttatggcaga agaaaaaatt tactttgatg
      601 agggaaatgt aaaagttacg aatgcgcgtt tcctgactta tggcaaaatg cactcaatgg
      661 ctaatgtgac ttcggtggcc aaatacgtta tcaagcctaa gcgtacgggt cctattgttt
      721 taggaataat tggcctaatc tgttttgcgt tcaaatggtg gcttgccgta ttgttgttgg
      781 ttggagcaat tgcatggtgg atgacgcaaa agaataaata tttggtttct ttggcgagtg
      841 cctcaggaag cgaagaggct ttaaccagtg ctgatgaggc cttcatcgac agggttgtca
      901 gtgcgctgaa tgaagccatt gcagaaagag gttgaagatg ttttacaaac ctgccggtaa
      961 ttgatttact ggcaggttcc tttaattgta aatttccgat cgaaaatcat tagtcgtttc
     1021 gtgttgtatt tagttccttt ggtaaaaaag gaagatagag agttatgtct ttatttgttc
     1081 ttcaagaatt atgagaacga aggggaatta tgcgaatagg gtttcttgcg gtattagttt
     1141 gtttatcaag agattataaa ttcaataaaa aatagttaaa ctatggccgg aaaaaatttt
     1201 caatttttca aagcctttcc cgaggatccc atatccaagg catgggataa ggaagaattg
     1261 atatatagcc ttgcttatg
//
LOCUS       DAORPD010000633         1276 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_21889_length_1276_cov_2.143325, whole genome shotgun sequence.
ACCESSION   DAORPD010000633 DAORPD010000000
VERSION     DAORPD010000633.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21889_length_1276_cov_2.143325"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1211)
                     /locus_tag="PK976_07430"
     CDS             complement(<1..1211)
                     /locus_tag="PK976_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007211715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HRC67755.1"
                     /translation="MINQQLLQPESIAIIGGSNDVTKPGGKVLKNLLDHHFKGKLYVV
                     NPKETKVQGLPCYNDARDLPPTDMAILAVPARLCPESVKILAETKSTKAFIILSAGFH
                     EESQEGARLEEEIVETVNKTGGCLIGPNCIGVMTPYHTSVFTTPIPELVPDGVDFISG
                     SGATAVFIMETAITNGLRFSSVFSVGNSAQIGVEEVLEYLDTHFDPLQSSKIKLLYIE
                     SIAKPDKLLKHASSLIEKGCKIAAIKAGTSAAGSRAASSHTGALSSSDTAVDALLRKA
                     GIVRCHSRQELATVGGIFTHPPLPGNRMAIITHAGGPAVMLTDALSNNGIEIPPLESP
                     ELLSKLYPGSSVANPIDFLATGTASQLAEIIDYCENRFDQIDAMAVIFGSPGLFEVYD
                     VYKVLDEKMRS"
BASE COUNT          343 a          288 c          283 g          362 t
ORIGIN      
        1 caactgcgca ttttttcatc caataccttg taaacgtcgt aaacttcaaa aaggcctgga
       61 ctaccaaata tcacagccat agcatcgatc tgatcgaaac ggttttcgca gtagtcgata
      121 atctcggcaa gctgactggc tgtgcctgtg gcaaggaaat cgataggatt ggctaccgat
      181 gaacctggat aaagtttcga gagaagctcg gggctttcga ggggcggaat ttctatacca
      241 ttgttggaga gcgcatctgt gagcataacc gccggaccac cggcatgtgt gatgatggcc
      301 atacgatttc ccggaagcgg tggatgagta aatattcctc ccacggtggc caattcttgc
      361 cggctatggc atcttacgat tccagcttta cgtaaaagtg catcaacagc cgtatcgcta
      421 ctggaaagag cacccgtatg tgaagatgct gcacgacttc cagcggcgct tgtccctgcc
      481 ttgattgccg ctattttgca tcctttctct atgagtgatg aagcgtgttt taatagctta
      541 tcaggcttag caatgctttc aatatataat agtttgattt tcgaactctg caaaggatcg
      601 aaatgtgtgt ccaaatattc cagaacctct tcaacgccaa tttgtgcact gtttcctacc
      661 gaaaaaacac ttgaaaatcg caatccattg gtaatagcag tttccataat gaaaactgcg
      721 gtagctccgc tgcccgagat aaaatctact ccatcgggta ccagttcagg aataggtgta
      781 gtaaagaccg atgtatgata gggtgtcatt acgcctatac agttgggccc aattaaacag
      841 cctccagttt tatttacggt ttcaacaatt tcctcttcca gccttgcgcc ttcctgactc
      901 tcttcgtgaa aacccgctga aagaatgatg aatgccttgg tgcttttcgt ttcagccaga
      961 atttttaccg attccggaca aagacgtgct ggaacggcca aaatagccat atcggtgggg
     1021 ggaaggtccc gggcatcatt ataacatggc aagccttgta ccttagtttc cttcggattc
     1081 acgacataga gcttcccttt aaaatggtga tcgaggaggt ttttcaatac ctttccaccg
     1141 ggtttggtaa catcgttgct tccaccaata atagcaatgc tctcaggctg aagtaattgt
     1201 tgattaatca taaattttac agttaagaag atttctcgat gaataataaa atgattcttt
     1261 ggcggcgaaa ttatga
//
LOCUS       DAORPD010000634         1276 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_21890_length_1276_cov_2.105651, whole genome shotgun sequence.
ACCESSION   DAORPD010000634 DAORPD010000000
VERSION     DAORPD010000634.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21890_length_1276_cov_2.105651"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="PK976_07435"
     CDS             <1..499
                     /locus_tag="PK976_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016276471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HRC67756.1"
                     /translation="QKNRPLVERVVTVTGTNVLHPSNFLVKIGTPVSALLEKAGWQPE
                     KAGKVIAGGPMMGKALLNADVPIVKGTSGILVLNGKESLRPKVQPCIRCAQCVSACPM
                     GLEPYYLARVSEVEKFDLAENGAILDCIECGSCQYICPSARPLLDHIRLGKLKVNQII
                     RSRNQ"
     gene            504..>1276
                     /locus_tag="PK976_07440"
     CDS             504..>1276
                     /locus_tag="PK976_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HRC67757.1"
                     /translation="MNPLIVSGSPHIHGEMTIKSIMWGVVIALLPAWLAGVWFFGIGA
                     VIVTLVSIASCVLFEFLIQKFVLKQEPTVNDGSAVVTGLLLAMNVPSSLPWWQVVIGS
                     LVAIGIVKMAFGGLGKNLFNPALIARVFLLISFPVTMTTWPLPIESRANVFNFDGVTG
                     PTPLGTLKTQLAMGQTVAQIKLPSYTNLLWGNIGGSFGEVSAIALIIGGIYMLYRKII
                     TWHIPVSFILTAFIFSGILCLVNPNRYADPVFHILAGGL"
BASE COUNT          342 a          246 c          287 g          401 t
ORIGIN      
        1 tcaaaaaaac aggcctttag tggaacgcgt ggttacggtt acaggaacca atgtgcttca
       61 tccatctaat tttttggtga aaatagggac tcccgtttcg gcattgcttg aaaaggctgg
      121 ttggcagcct gaaaaggccg gaaaggtaat agcaggtggg cctatgatgg gtaaagcctt
      181 attaaatgca gacgtgccca ttgttaaggg aacttcgggt attcttgtgt tgaacggtaa
      241 agagtcttta cgaccaaagg ttcagccttg tatccgctgc gctcagtgtg taagtgcctg
      301 ccctatgggg ctcgaacctt attatctggc aagagtttca gaagtagaaa aattcgactt
      361 ggctgaaaat ggagctatac ttgattgtat tgaatgtggc tcatgtcagt atatctgtcc
      421 ctcagcccga ccacttctcg accatatccg attgggaaaa ttgaaagtca atcaaattat
      481 tcgatctcga aatcaataac aatatgaatc cattgattgt atctggttca ccacatatac
      541 atggtgaaat gacgataaaa tccatcatgt ggggagtagt aattgcactt cttcccgcat
      601 ggctggcagg cgtatggttc tttggaattg gtgcagtcat tgtaaccctg gtatcaatag
      661 cctcatgtgt attgttcgaa tttcttatac agaaatttgt cttgaaacag gaacctaccg
      721 ttaatgatgg ttcagccgtt gttacaggcc tgcttttagc catgaatgtt cccagctcat
      781 taccttggtg gcaggtcgta ataggtagtt tagtagctat tggaattgta aagatggcct
      841 ttggcggctt gggtaaaaat ttattcaatc ccgccttgat cgcaagggtg tttttactga
      901 tttcatttcc ggtaaccatg actacttggc cattgcccat cgaaagccga gccaatgtat
      961 ttaatttcga tggagttact ggccctacac ctttaggaac tttaaagacc caattggcta
     1021 tggggcaaac agtagctcaa atcaaacttc cttcttatac gaatttatta tggggtaaca
     1081 ttggaggatc gtttggcgaa gtatcggcca ttgcactgat tattggagga atttatatgc
     1141 tctaccgcaa aatcattact tggcatattc cagtaagttt tatactgaca gcatttatct
     1201 tttctggaat tctatgctta gtaaatccaa atcgatatgc tgatcctgta tttcatatat
     1261 tagccggtgg tttatt
//
LOCUS       DAORPD010000635         1270 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_22065_length_1270_cov_2.888066, whole genome shotgun sequence.
ACCESSION   DAORPD010000635 DAORPD010000000
VERSION     DAORPD010000635.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22065_length_1270_cov_2.888066"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="PK976_07445"
     CDS             <1..497
                     /locus_tag="PK976_07445"
                     /inference="COORDINATES: protein motif:HMM:TIGR03511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility lipoprotein GldH"
                     /protein_id="HRC67758.1"
                     /translation="FNKTTPQMRIYSFLCMSLFSILMISCQTNDVFNGMEKIPNAKWN
                     NNHQVTFQTFIQDTLIPLDVYVQLRNNQYYPYSNIYLFLTTISPDRTTYRDTLECILA
                     SPEGQWLGKRTGTVFESRFLIKHNVLFARSGPYVFKISHGMRESEITGIIGVGFRISK
                     PKTN"
     gene            592..>1270
                     /locus_tag="PK976_07450"
     CDS             592..>1270
                     /locus_tag="PK976_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HRC67759.1"
                     /translation="MDFYIKRMWQIYAGLIAVILLFFILLSAGVFGEMPSFEDLENPK
                     SNLASQVISADGKVLGTYYIQNRSNIHFNELSPYLIQALIATEDARFEKHAGIDYKAL
                     LRVSAGVLTGRYAGGGSTITQQLAKNLFPRGQNLSTPAFVLKKFKEWVTAVKLERNYT
                     KEEILAMYLNTVDFGSNAYGVKSAANTFFSKEPRELNAEESALLIAIVNAPTRYSPVR
                     NPENALKR"
BASE COUNT          389 a          274 c          234 g          373 t
ORIGIN      
        1 tatttaataa gacaaccccc cagatgagaa tttattcatt tttatgcatg agccttttta
       61 gcattttgat gatatcatgt caaacaaatg acgtttttaa cggcatggaa aaaattccta
      121 atgctaaatg gaacaataat catcaggtca ccttccaaac ctttattcag gatactctca
      181 ttccgctcga tgtttatgtg cagttgcgca ataatcaata ttatccctat tccaacattt
      241 atcttttttt aactaccatt tcccccgatc ggacaaccta ccgtgatacc ttggaatgca
      301 tacttgctag ccccgaagga caatggctgg gcaaaagaac aggaacggtt ttcgaaagcc
      361 gttttctcat caagcataat gtacttttcg cacgaagcgg accttatgtt ttcaaaattt
      421 ctcatggcat gcgtgagtca gaaataaccg gaatcatcgg agtgggattc cgaatatcaa
      481 agcctaaaac caactaaata tcatttatca tttttaattg atgtttcaat ggcttcgaaa
      541 gaaaattttt ttaggaaata ttttaccaat caagggaaat atccaaaaga aatggatttt
      601 tacattaaac gaatgtggca gatatatgca ggcttgattg cagtgattct gctttttttt
      661 attttacttt cagccggtgt ttttggggag atgccctcgt ttgaagacct cgaaaatcct
      721 aaaagcaatc tggcttctca ggtaatttca gccgacggga aagttttggg aacttattat
      781 atacaaaacc gttcaaatat ccatttcaat gaattatcgc cctatctcat tcaagcactt
      841 atagctactg aagatgctcg tttcgaaaaa catgccggga ttgactataa agcccttcta
      901 agggttagtg caggagttct tacaggacgc tatgccggag gaggaagcac cattacacaa
      961 caattggcta agaatctttt ccctcgcgga caaaacctct ctaccccagc atttgttctg
     1021 aaaaaattca aggaatgggt tacagccgtt aagctcgagc gtaactacac aaaagaagag
     1081 atccttgcta tgtatttaaa taccgtggat ttcggaagca acgcttatgg cgtaaaatcg
     1141 gccgccaata ccttcttctc gaaggaacct cgtgaactga atgcagaaga atcggcctta
     1201 ttgatcgcaa tcgtgaatgc ccctactcgt tacagccctg tccgaaatcc cgaaaatgcc
     1261 ctcaaacgcc
//
LOCUS       DAORPD010000636         1269 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_22105_length_1269_cov_2.481878, whole genome shotgun sequence.
ACCESSION   DAORPD010000636 DAORPD010000000
VERSION     DAORPD010000636.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22105_length_1269_cov_2.481878"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..112)
                     /locus_tag="PK976_07455"
     CDS             complement(<1..112)
                     /locus_tag="PK976_07455"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009235 - cobalamin metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP:cob(I)alamin adenosyltransferase"
                     /protein_id="HRC67760.1"
                     /translation="MGKGKKIYTHTGDQGETSLIGGQRVPKSHEKIEAYGT"
     gene            complement(233..964)
                     /locus_tag="PK976_07460"
     CDS             complement(233..964)
                     /locus_tag="PK976_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005786909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC67761.1"
                     /translation="MMNEEIIRLINIKKYYKVGAEIVKALQSITMSVNRNEYLALMGA
                     SGSGKSTLMNIIGCLDTPTDGQYFLEGRDVSKMDDNQLAEIRNKKIGFVFQTFNLLPR
                     SNALDNVVLPLIYAGVSKEERIARGKEVLEQVHLADRMMHKPNELSGGQRQRVAIARA
                     LVNRPSIILADEPTGNLDSKTSLEILALFQEIHEAGNTIILVTHEEDIAKHAHRIVKL
                     RDGLIESDYPNLEIHRLPSTFASAL"
     gene            complement(1023..>1269)
                     /locus_tag="PK976_07465"
     CDS             complement(1023..>1269)
                     /locus_tag="PK976_07465"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRC67762.1"
                     /translation="LVFIDGNHNKESTLNYFGGILPSLTPQSILIFDDIHWSKPMEEA
                     WENVQQNPLVRVCIDLYWMGLVFVNTALSKQNYILRI"
BASE COUNT          346 a          254 c          269 g          400 t
ORIGIN      
        1 gtgtaccgta agcttcgatt ttttcgtgcg atttaggtac tcgctgtccg ccgatgagag
       61 atgtttcacc ttggtcgcct gtatgtgtat aaattttttt cccttttccc atggaataat
      121 tttatttaag gcaatcgaga aatctttcga tgtcagatta tttcatgcat gtttcattta
      181 aaaaaacgag tcttcagttc atttattaga attataatct acgatataga aactataaag
      241 ccgaggcgaa ggtagatggc aggcggtgta tttcgagatt gggataatcg gattcgatga
      301 gtccatcgcg cagttttacg atacgatggg cgtgtttggc aatatcctct tcgtgggtaa
      361 ccaaaattat cgtattgcct gcttcgtgta tttcctggaa aagagccaaa atttcgagag
      421 aggttttcga atcgaggttt ccggtgggtt cgtcggcaag aataatagaa ggcctgttga
      481 ccagagctct ggcgatggct accctttgtc gttgtcctcc cgaaagctcg ttgggtttat
      541 gcatcatacg gtcggccaaa tgtacctgtt cgagcacttc cttgccgcgg gcaattcgtt
      601 cttctttcga aacgcctgca taaatcaaag gcaatacaac attatcgagt gcgttgctac
      661 gaggcaaaag attgaaagtt tgaaacacaa aaccaatctt cttgtttctg atttcagcaa
      721 gctggttgtc gtccatcttg cttacatcgc ggccttccag aaaatactga ccatcggttg
      781 gcgtatcgag gcatccgatt atgttcatca aggttgattt accagaaccc gaagcaccca
      841 tcaaggctaa atattcgttc ctgttcacac tcatggtgat ggattgcaat gccttaacta
      901 tctcggctcc taccttgtag tatttcttta tattgattag tcttataatt tcttcgttca
      961 tcatttaagc ggattaagct acaaaagtaa ccattatcat tgtgaaagaa acaatcgaaa
     1021 aactatatcc ggagaatata attttgttta ctaagagctg tattaacaaa aaccaatccc
     1081 atccaataaa ggtcgataca aactctaacc agaggatttt gttggacgtt ttcccaggct
     1141 tcttccatag gtttcgacca gtggatatca tcgaaaatta gaatgctttg tggagtgagt
     1201 gaaggcaaaa ttccaccgaa ataattcagg gttgattctt tgttgtgatt cccgtcaatg
     1261 aatacgagg
//
LOCUS       DAORPD010000637         1266 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_22191_length_1266_cov_2.317093, whole genome shotgun sequence.
ACCESSION   DAORPD010000637 DAORPD010000000
VERSION     DAORPD010000637.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22191_length_1266_cov_2.317093"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1266
                     /locus_tag="PK976_07470"
     CDS             <1..>1266
                     /locus_tag="PK976_07470"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HRC67763.1"
                     /translation="AFNTQTKRFQFDKHPYSDFAYCYITINNSKGKRIQLNPLPEVEA
                     TQTITTFTAYIHHEEDLINLGKSGRQWYGESFDIQNTMNFAISLEGVVNGSRVLLESA
                     VVASSYSISNMKISVGETPFLVQTISPIPPPSLDSDYGKRSIDTASFKANSTTVSLSY
                     KYIKPLNSSQAWLDYFTINYQRNLSFSGSQMSFRKPMHYSSTQVVRYKIGNASQNIQI
                     WEVTNALRPMRMQTQLAGNTLTFDSKNDTLREFIAFDGSKFLNPTFIGKVPNQNLHGM
                     IDADYIIISHPDFLSQAQELANIHESENQFNVKIVTPEMVYNEFSSGIQDPSAIRDFM
                     RHLRLSSGNIRKPAYLLLFGDASYDYKDRIQGNTNFVPTYQSDESLNPSYSFATDDYF
                     TFLDQSEGKGGGGTPDLCVGRIPVFTQQQ"
BASE COUNT          383 a          276 c          232 g          375 t
ORIGIN      
        1 gggctttcaa tacccaaaca aaacgttttc agttcgataa acatccttat tccgatttcg
       61 cttattgtta tattaccatc aacaattcta aagggaaaag aatacaatta aatccacttc
      121 cagaggtcga agccactcaa accataacca cttttacggc ttatattcat catgaagaag
      181 acttgatcaa tcttggtaaa agtggaaggc aatggtatgg tgaatccttc gatatacaaa
      241 acacgatgaa tttcgcgatt tccctcgagg gagtggttaa cggaagccgt gttttactcg
      301 aatcggctgt ggtcgcaagt tcttattcta tcagcaatat gaagatttca gtgggagaaa
      361 ctcctttcct tgttcaaact atttctccta ttcctccccc ttctctcgat tcagattatg
      421 ggaaaagaag catagacaca gcctcattta aagccaatag tacaacggtt tcactttcct
      481 ataaatatat caagcctttg aattcttctc aggcctggct cgattatttt accattaact
      541 atcaacgtaa tctgagtttt tcgggttcac agatgagctt tcgtaaaccc atgcattatt
      601 cgagtaccca agttgtacga tataagatag gaaatgccag ccagaacatt cagatatggg
      661 aggtgaccaa tgccttacgg cctatgagga tgcaaaccca gcttgcggga aatacgctta
      721 ctttcgattc gaaaaacgat accttgcgtg aatttatcgc ttttgacgga agcaaatttc
      781 taaacccgac ttttattggt aaagttccaa atcaaaatct tcatgggatg atcgatgcag
      841 attatatcat cataagccat cccgatttct tgtcacaagc tcaggaactt gctaatatcc
      901 atgaatcaga aaatcaattc aatgtaaaaa ttgtaactcc cgagatggtt tacaatgaat
      961 tttcgtcggg catacaagat ccaagcgcca ttcgtgattt tatgaggcat ttgaggttga
     1021 gttcgggcaa cattcgtaaa ccagcatatc ttctactatt tggagacgcc tcttacgatt
     1081 ataaagaccg cattcaggga aataccaact ttgtacccac ttaccaatcg gacgaatcct
     1141 taaatccttc ttattctttt gctacagatg attattttac ctttctcgac caaagcgaag
     1201 ggaaaggtgg aggtggaact cccgatctat gtgttggccg aatacccgtt ttcacccagc
     1261 aacaag
//
LOCUS       DAORPD010000638         1263 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_22275_length_1263_cov_2.009106, whole genome shotgun sequence.
ACCESSION   DAORPD010000638 DAORPD010000000
VERSION     DAORPD010000638.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22275_length_1263_cov_2.009106"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..880
                     /locus_tag="PK976_07475"
     CDS             <1..880
                     /locus_tag="PK976_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67764.1"
                     /translation="YHPHAYHAGSVWPLFTGWHAMAAYKANAFETGFRLIMANMNIFR
                     HWGYGYSEEVLNGDVYEPAGVCRHQCWSETMAIQPVAEGMLGFEPNALQNILRIAPAL
                     PPHWNSIHIHNLRFKESLIHFTMHTDDFKQSWQFNIHGNCSPQVIFAPYFPVGSRINS
                     IAINGQSAHFEEVINERYTQIVIYFKLDNSTRIEINYEPGISIIPEINLPEPGDKADG
                     LRIIQSYIVDSVYVIEIEAPQNTQHTIKIKTSGERQYLIDGGYLKSCKDRIIEIGVNF
                     QDSEKPYVRKKIRLKI"
     gene            complement(947..>1263)
                     /locus_tag="PK976_07480"
     CDS             complement(947..>1263)
                     /locus_tag="PK976_07480"
                     /inference="COORDINATES: protein motif:HMM:NF027726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:2001070 - starch binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SusF/SusE family outer membrane protein"
                     /protein_id="HRC67765.1"
                     /translation="LYILGDGTPAAWDNNNAIPMTKLAPGIFEISIELGGGGKWLKFI
                     EVLGQWAPQYGTNETGTSTGGPLVLRPTESEPDPLSIPCPPTAGTYTVKVDLATMTYS
                     IK"
BASE COUNT          397 a          255 c          269 g          342 t
ORIGIN      
        1 ctatcatccc catgcttatc acgccggctc ggtatggccg ctctttaccg gatggcatgc
       61 tatggcagct tacaaagcga atgctttcga aactggattt aggcttatca tggcgaacat
      121 gaatattttt cgtcattggg gctacggata ttcagaggag gtattgaacg gagatgtata
      181 tgaacctgca ggtgtatgtc ggcatcaatg ctggtcggag acaatggcca ttcagcctgt
      241 ggcagaagga atgctgggct tcgaaccaaa tgccttgcaa aatatccttc ggattgcacc
      301 agcattgcct ccccactgga attcaatcca tatccataat ctccgattca aagaatcgct
      361 cattcatttt actatgcata ccgatgactt caaacaaagc tggcaattca atatccatgg
      421 aaattgttcg ccccaagtta tttttgctcc ttatttccca gtaggaagcc ggataaattc
      481 gatagcaata aatggacaat cggctcattt cgaagaggtt ataaatgaaa gatatactca
      541 aattgtaatt tatttcaagc tggataattc tactcggata gaaataaatt atgaaccagg
      601 gatttctatt atcccagaaa ttaacttacc cgagccagga gacaaagcag atggactgag
      661 aatcatacaa tcttatatag ttgattcggt ttatgttata gaaattgaag caccacaaaa
      721 cactcaacat actattaaaa taaagacatc aggcgaaagg cagtatttga ttgacggtgg
      781 gtatttaaag agttgcaaag atagaattat agaaatcgga gtcaactttc aggattccga
      841 aaaaccttat gtaagaaaaa aaatcaggct taagatataa tagagacaaa aaagccgtcc
      901 agtcttattg attggacggc taagctcaat aatcaagcta tcggaattac ttgatagaat
      961 atgtcatagt agccaggtcg accttaacgg tgtaggtacc tgctgtagga ggacaaggga
     1021 tagaaagtgg gtcaggttca ctttcagtag gtctaagaac aagtggccca ccagttgaag
     1081 ttccagtttc attggttcca tactgtggag cccattgtcc gagaacctca atgaatttca
     1141 gccatttacc accaccgcca agctctatgc ttatctcgaa gatgcccgga gccagtttgg
     1201 tcattgggat agcattattg ttatcccaag cggcaggagt accatctcca aggatataaa
     1261 gct
//
LOCUS       DAORPD010000639         1262 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_22316_length_1262_cov_2.096935, whole genome shotgun sequence.
ACCESSION   DAORPD010000639 DAORPD010000000
VERSION     DAORPD010000639.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22316_length_1262_cov_2.096935"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            38..706
                     /locus_tag="PK976_07485"
     CDS             38..706
                     /locus_tag="PK976_07485"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HRC67766.1"
                     /translation="MPVNRIQVLNNGNILGLWQITESSEELIPFACLDPDEFVQFSKF
                     KSPLRRLHWLAWRALLRKMIPESNFSLIYDDNGKPFPLDHSFQLSVSHSGNWATCFID
                     ANHPVGLDLEAVHERVLKVKERFLSPLELEYFSPSANDPFVFTVLWSAKEAVFKYIGQ
                     KGINFRQQITIEPFSPYESHSFFVNIHHNSLAKKLVLKWQNIDSKYLLVHTQCQEKHS
                     IMSC"
     gene            706..>1262
                     /locus_tag="PK976_07490"
     CDS             706..>1262
                     /locus_tag="PK976_07490"
                     /inference="COORDINATES: protein motif:HMM:NF025647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4369 domain-containing protein"
                     /protein_id="HRC67767.1"
                     /translation="MTIALFSGFLIFESCHPSSAEQPSPLVIQGFLQDELQSKIVLHE
                     MDIIEMHPVDSVMTGKDGSFVFSLFITEPVFYQMSIGKNDPLVLIVNPGEKIQLKGKA
                     DSLIFADIQGSEESRLMQEYLIHTQRNLRLSESLATLLAVKSQEKDFLTVRDSILKVF
                     DSIYTDQKQFVKNFILHHPGSLASL"
BASE COUNT          371 a          270 c          235 g          386 t
ORIGIN      
        1 ttacttttgc agagtcgatt catgaaatta tgcagcaatg ccagtaaaca ggattcaagt
       61 acttaataat ggaaacattc tgggtttatg gcaaatcaca gaatcatcag aggaactgat
      121 tccgtttgct tgtttagatc ctgatgagtt cgtgcaattc agtaaattca aatcaccctt
      181 acgcaggctt cactggctgg catggcgggc cttacttcgc aagatgattc ccgaatccaa
      241 tttttcttta atttatgatg acaatgggaa acccttcccc cttgatcatt cttttcaact
      301 ttcggtaagt cattccggga attgggctac ctgctttata gatgccaacc atcctgttgg
      361 gctcgacctc gaggctgttc atgaaagagt gctcaaagtg aaagaacgct tcttatcacc
      421 attagaactc gaatatttct cgccatcagc caatgacccc ttcgtattta ctgttttatg
      481 gtcggctaaa gaagctgtct tcaaatatat cggacaaaaa ggtatcaatt tccgacaaca
      541 aatcactatc gaaccctttt ctccatacga atctcattca ttcttcgtaa atatccatca
      601 caattcattg gcaaagaagc tcgttttaaa atggcaaaac atagatagca aatatctctt
      661 agttcatacc caatgtcaag aaaaacattc cataatgagc tgttgatgac tatagcccta
      721 ttctcgggtt ttctgatttt tgaatcctgc catccaagct cggctgagca accatcccct
      781 ttggtaattc aaggatttct ccaagatgag ttgcagtcca aaatcgtgct tcatgaaatg
      841 gatattatcg aaatgcatcc tgtcgattcc gtgatgacag ggaaagatgg cagttttgtg
      901 ttctccttat tcattaccga gcctgttttt tatcagatga gtattgggaa aaacgatcct
      961 cttgttctga ttgtgaatcc tggtgagaaa attcaattga aaggcaaagc cgacagtttg
     1021 atttttgccg atatacaggg ttcggaagaa agcaggctta tgcaggaata ccttattcat
     1081 acacaacgga atttacgatt atcggaatca ttagctacat tgctggcagt aaaaagccaa
     1141 gagaaggatt ttctaacggt acgtgattcc atcctcaaag tgtttgattc aatatatacc
     1201 gatcaaaaac aattcgtaaa aaacttcatt ctgcatcatc ccggctcact ggcttcgcta
     1261 at
//
LOCUS       DAORPD010000640         1261 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_22342_length_1261_cov_2.803483, whole genome shotgun sequence.
ACCESSION   DAORPD010000640 DAORPD010000000
VERSION     DAORPD010000640.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22342_length_1261_cov_2.803483"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="PK976_07495"
     CDS             <1..374
                     /locus_tag="PK976_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HRC67768.1"
                     /translation="VANLNNTYKTIKMWNYPQSRFIDTGLQFCGLVLGDHSKTGINTT
                     FNTGSVVGISSNVFGAGYQRNFVPSFVWGGPSTGYSGYDFEKAIETAKEVYKRRGIEL
                     TDVDFKILQHVYEITKNNIRL"
     gene            complement(466..630)
                     /locus_tag="PK976_07500"
     CDS             complement(466..630)
                     /locus_tag="PK976_07500"
                     /inference="COORDINATES: protein motif:HMM:NF028380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein THX"
                     /protein_id="HRC67769.1"
                     /translation="MGKGDKKSRRGKIVIGTYGVRRPKKKKKHAITNPIEVESVLSDK
                     LQKKSKKKAE"
     gene            complement(680..>1261)
                     /locus_tag="PK976_07505"
     CDS             complement(680..>1261)
                     /locus_tag="PK976_07505"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRC67770.1"
                     /translation="RHFAVVLENIFQSHNISAVLRSCDCFGVQDVHIIENFNPYRVNP
                     EIALGSSKWLTIYHHGDGVNAVVKAFDDIHDKGYQIIAASPHAGGYDIDSLNIENKTA
                     LIFGTEKDGLSPEALQRADGYLKIPMYGFTESLNISVSAGISLYTLMSRLHKTSIPWQ
                     LSEPEALEIKLSWAIQSIKHAEYLIYQFLSKYE"
BASE COUNT          369 a          249 c          237 g          406 t
ORIGIN      
        1 atgtggctaa tctcaataat acctataaaa ctattaagat gtggaattat cctcaatctc
       61 gctttataga tactggatta cagttttgcg ggcttgtatt gggcgatcat tccaaaacag
      121 gaatcaacac tacctttaat acgggttcgg tagtgggcat tagctcgaat gtgtttggtg
      181 ccggatatca acgcaatttt gtaccatctt tcgtatgggg aggccctagt acaggttatt
      241 caggctacga tttcgaaaag gccatagaaa ccgcaaagga agtctataaa cgacgcggta
      301 ttgaactcac cgatgtcgac ttcaaaatcc tccaacatgt atacgaaatc acaaagaata
      361 atataaggct ttaatttcac acccaaataa aaatgaaatc ggtatctctg aaagtcttct
      421 gccgagatac cgattctttt gtaaagcgaa atatgatctc aggtcttatt ctgctttttt
      481 cttgcttttt ttttgaagct tgtcagaaag aactgattcg acttcaatgg ggtttgtaat
      541 agcgtgcttc tttttctttt taggccggcg cactccatac gttccaatga cgatttttcc
      601 tcttctgctt ttcttatccc cttttcccat aatttagatt ttaagaattg attcgaattt
      661 gagggtaaaa ttaagaagtt tattcgtact ttgataaaaa ttgataaata aggtattcgg
      721 catgctttat ggactgaatt gcccaactca gtttgatttc taaagcttcg ggttcgctca
      781 gttgccatgg aatgctcgtc ttgtgtaatc gagacatcaa ggtataaagt gaaattcctg
      841 ccgaaacgct aatattcaaa ctttcggtaa atccatacat ggggatcttc aggtaaccat
      901 cggctctctg gagagcttct ggacttaaac catctttctc ggtgccaaaa attaatgcag
      961 ttttgttttc gatgtttagt gaatcaatat cataaccccc tgcatgaggc gaagccgcta
     1021 tgatttgata acctttgtca tgaatatcat cgaaagcttt aacaaccgca ttgactccat
     1081 caccgtgatg gtatatggtc aaccattttg atgatcccag cgctatttcg ggattaacac
     1141 gatacggatt aaaattttcg ataatatgga catcctgaac tccaaaacaa tcacatgatc
     1201 ttaaaactgc gctaatatta tgcgactgga aaatattttc gagtacaacg gcaaaatgtc
     1261 t
//
LOCUS       DAORPD010000641         1260 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_22362_length_1260_cov_2.004149, whole genome shotgun sequence.
ACCESSION   DAORPD010000641 DAORPD010000000
VERSION     DAORPD010000641.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22362_length_1260_cov_2.004149"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..>1260)
                     /locus_tag="PK976_07510"
     CDS             complement(67..>1260)
                     /locus_tag="PK976_07510"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase"
                     /protein_id="HRC67771.1"
                     /translation="IIYDSDIDRSFFRHPLSILIIIHLAWMLITCLSSELPLVSFKYL
                     LSQLWFIVPMYFMGILYFKNEKNIRRFIRVYIASLLIVIVYTTIRHAKVGFDEEIGHW
                     VMYPFYNDHTAYGAITALLIPVSFSLSLDKSHGRFWQWFAIISTIMLSIGLYLSFSRA
                     AWIGLLGGVIVFILVLLKIPFKWILTTLILVIGLFFAFQQQIIDRLEKNKQDSSANFV
                     EHVKSIANIRTDASNLERINRWQAALRLYKERPIVGWGPGTYQFIYAPYQLSKEKTII
                     STNAGDKGNAHSEYIGPLAERGLLGTILIVLIFSFSIYYGFRVYYKASSRHHRQLAIG
                     IIIGLITYYIHGFMNNFLDTDKLSVPFWAFYAMIVSLDMYYTNKKNSPRSDISTPPLE
                     KPTDS"
BASE COUNT          421 a          224 c          288 g          327 t
ORIGIN      
        1 acgagggcga tcaaaaacaa aaggatggtt ttttttacgg gcataaggtt tacagagaga
       61 taattcttaa gaatcggtag gtttttccaa aggaggggtc gagatatccg atcgagggga
      121 atttttttta ttggtataat acatatcgag tgaaacgatc atagcataga aggcccagaa
      181 tggcacgctc agtttatcag tatcgaggaa attattcatg aagccatgaa tataataagt
      241 aatcaagccg atgatgatgc cgatagccag ttgtcggtga tgtcgtgagc tagctttgta
      301 gtaaactctg aaaccatagt agatagaaaa agaaaatata agaacaatga gaattgtacc
      361 taagagtcct ctttcagcaa gtggcccgat atattcgctg tgggcattgc ctttatcgcc
      421 agcattggtc gaaatgatgg ttttttcttt agaaagttga taaggagcat aaataaactg
      481 ataagttccc ggaccccatc caacgattgg cctttccttg tacaaacgaa gtgcagcttg
      541 ccaacgattg atacgttcga gattggatgc atcggttcgg atattggcta tggatttaac
      601 gtgttctaca aaattggccg aagaatcttg tttgttcttt tcgaggcgat cgatgatttg
      661 ctgctgaaag gcgaagaaga gcccgatcac gaggatgagt gtggttaaaa tccatttgaa
      721 cggaattttt aaaagaacca gaataaaaac gataactccg cctaaaagtc caatccatgc
      781 agcgcggcta aacgaaaggt acaatccaat tgaaagcatg atggtggaga taatggcaaa
      841 ccattgccag aagcggccat ggctcttatc gagtgagagg gaaaaactta cagggattag
      901 taaagcagta atggctccat aggccgtatg gtcattgtaa aaaggataca tcacccagtg
      961 ccctatttct tcgtcgaaac ccaccttggc atggcgaatg gtcgtataaa ctatcacaat
     1021 caataaagaa gcgatatata ctcgaatgaa tcttcgaata ttcttctcgt tcttaaagta
     1081 tagaattccc ataaaataca tgggcacaat aaaccacaat tgagagagaa ggtatttgaa
     1141 tgatactaaa ggtaactcgc tcgacaaaca ggtaatcaac atccacgcca gatggataat
     1201 aatgagtatg ctcaaaggat gacggaaaaa cgagcggtca atgtcgctat cataaatgat
//
LOCUS       DAORPD010000642         1259 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_22398_length_1259_cov_2.107973, whole genome shotgun sequence.
ACCESSION   DAORPD010000642 DAORPD010000000
VERSION     DAORPD010000642.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22398_length_1259_cov_2.107973"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="PK976_07515"
     CDS             <1..431
                     /locus_tag="PK976_07515"
                     /inference="COORDINATES: protein motif:HMM:NF025710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4402 domain-containing protein"
                     /protein_id="HRC67772.1"
                     /translation="RTSELKVHVSQELSFGSFYSGRSGGTITISPDGNRWVTGEIILL
                     VNSSGCPALLEIEAPPNRTIHVEFPVSAEIKQAGGQQSLTINNFTTDKLNNSLLTSSR
                     DLPYGNQVRVGATLTVPGSVTLSSGNYIGNFRINVVCIQE"
     gene            438..>1259
                     /locus_tag="PK976_07520"
     CDS             438..>1259
                     /locus_tag="PK976_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67773.1"
                     /translation="MHNNKSFFCYLHFLLGKAIHQFPILWILWLAFFISNPLKINCQN
                     NQSEELPAIEEIPVSIRIESVGAGMMNSLYEYESEKLFLPVLELFRFLQIKAEPSVTL
                     DSISGFIGKEEYRYLIDNHYKKIFFRGKDFSIAENQSMNTGDDLYIDSRLFGEIFGLH
                     CKFNFRNLSVTIKPDFELPVIRKMRIEQFRKNIKQLRGEVEADTTLKHQHELWKFGMM
                     DWAATSTQSTDSKTSSQFWLRTGAEALYGETNLQLYYSSLNEFTNRNLQYYWRWVD"
BASE COUNT          393 a          242 c          254 g          370 t
ORIGIN      
        1 aaaggaccag cgaattaaag gttcatgtaa gtcaagaatt atcattcggt tcattttata
       61 gtggacgtag tggtggaact attactattt ctccagatgg caatcgatgg gtcacgggtg
      121 aaattatctt attagtcaat tcgagtggat gtcctgcttt attagaaatt gaagcacctc
      181 ctaaccgtac cattcatgtt gaatttcccg tttctgcaga aataaagcag gcagggggtc
      241 agcaaagcct tactatcaat aattttacca ccgataaact caacaatagt ttattaactt
      301 cctccagaga ccttccttac ggaaatcagg ttagggtcgg ggcaacgctt acagtaccag
      361 gctcagtcac cctttcttcc ggaaattata ttggcaattt cagaataaat gtagtatgta
      421 ttcaggaata gattaatgtg cataacaaca aatccttctt ttgctacctt catttcttat
      481 tgggaaaagc catacatcaa tttcctatcc tgtggatttt atggctagca ttttttattt
      541 cgaatcctct caaaataaat tgtcaaaaca atcaaagcga agaactacca gcgatagaag
      601 aaattcctgt cagcatacgg atagaaagtg taggggcagg tatgatgaat tccctctatg
      661 agtatgaatc ggagaagctg tttttgcccg tattagaatt attccgattt cttcagataa
      721 aggcagaacc ttcggttaca ctcgattcta tcagcggttt cataggaaaa gaggagtacc
      781 gctatcttat tgataaccat tacaaaaaga tctttttcag ggggaaagat ttttccatag
      841 ctgaaaatca gtcgatgaat acgggagacg atctttacat agattctcgt ctttttggtg
      901 aaatattcgg tttgcattgc aagttcaact ttcgcaatct ttctgttacg ataaaacccg
      961 atttcgaatt gcctgtcata aggaaaatga ggatcgaaca attcagaaag aacatcaaac
     1021 agttaagagg tgaggtagaa gcagatacta ctttaaagca ccagcatgag ctttggaaat
     1081 tcggtatgat ggattgggct gccacttcga cacaaagcac cgacagcaaa accagcagcc
     1141 agttttggtt aagaaccggt gccgaagcat tatatggcga aaccaatttg cagctttatt
     1201 attcctcgct taatgaattt acgaaccgca acttacaata ctactggcga tgggtggac
//
LOCUS       DAORPD010000643         1259 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_22404_length_1259_cov_1.686047, whole genome shotgun sequence.
ACCESSION   DAORPD010000643 DAORPD010000000
VERSION     DAORPD010000643.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22404_length_1259_cov_1.686047"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1259)
                     /locus_tag="PK976_07525"
     CDS             complement(<1..>1259)
                     /locus_tag="PK976_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009126558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HRC67774.1"
                     /translation="RVVEGPKATIKRVIISGNDRLYEDVIRRELRTKPGAVYSQEDVI
                     RSIRELAQTGHFDPENLQPDINPNFDDGTVDIGYPLTSKGNDQIEFSAGWGVTGLVGK
                     LSLKLNNFSVRNLLNPGSYKGFIPQGDGQTLILSAQTNGQYYQSYQFSFIEPWFEGKR
                     PNNFSVGAYYSRYTGMNSNYYSSSMANYGMYNPYGYGYGGYGYGYGGYGYEDMAQYAA
                     DPDKVFSIFGVSAGYGKRLEWPDDFFMLQGELAYQRYSLKNWYGYNSFPFLTGVSNSI
                     SLNLTLARNSIDNPIYTRTGSQFTLSANLTPPFSLWDGKDYANMKTDAMGYEDPSKYT
                     WNEYHKWKLKIRTFTPLTSLTVKRTPVIATRVELGLVGHYNKHKITPFETFDVGGDGM
                     SGYSSSFATENIALRGYENNSIVMEGRA"
BASE COUNT          320 a          278 c          278 g          383 t
ORIGIN      
        1 gctcgacctt ccataacgat tgaattgttt tcgtatcctc tcagagcaat attctccgtc
       61 gcaaaagagc ttgagtatcc ggacataccg tcacctccaa cgtcaaaggt ttcgaaaggc
      121 gttattttat gcttgttata atgccccacg agtcccaatt ctacacgcgt ggcaatgacg
      181 ggtgttcgtt ttaccgtaag cgatgtgaga ggcgtgaatg tgcgtatttt gagtttccac
      241 ttatgatatt cattccacgt atatttcgaa ggatcttcat atcccattgc atctgttttc
      301 atgttagcat aatctttccc gtcccataac gaaaatgggg gcgttaggtt tgccgaaagt
      361 gtaaattgcg aaccggtacg tgtgtaaata gggttatcga tcgaatttcg agctaatgtg
      421 aggtttagtg atatgctatt gctcacgccg gtcaggaaag gaaaagagtt gtaaccatac
      481 cagtttttga ggctatatcg ttgataagcc agttctcctt gcagcatgaa gaaatcatcc
      541 ggccattcaa gtcttttccc atagcctgcc gaaacaccga aaatgctgaa tactttatcg
      601 gggtcggctg catattgtgc catatcttca tacccatagc ctccgtatcc gtatccataa
      661 cctccatacc catatccata gggattatac atgccataat tggccatgct gctggaataa
      721 tagttggagt tcatgccggt gtaacgtgaa tagtaggccc cgacagagaa gttgtttgga
      781 cgttttcctt caaaccatgg ttcgatgaat gaaaattggt atgattgata atattgaccg
      841 ttagtttgtg cactcaggat gagcgtttgt ccatcgccct gtgggataaa tcctttgtat
      901 gaacccggat tgagaaggtt tcttacggaa aagttgttca gttttaaact tagtttgcca
      961 accaatccgg taacacccca tccggcagaa aattcgattt gatcatttcc tttcgaagta
     1021 agaggataac caatgtccac ggtcccgtcg tcgaaattgg gattgatgtc cggttgaaga
     1081 ttttcgggat cgaaatgtcc ggtttgagcc agctcgcgga tcgatctgat tacgtcttcc
     1141 tgactgtaca ccgctccagg ttttgtcctt aattctctac gaattacgtc ttcgtaaagg
     1201 cgatcgtttc cggaaataat cacacgttta atagtggctt tcggcccttc tacaacacg
//
LOCUS       DAORPD010000644         1256 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_22482_length_1256_cov_2.427977, whole genome shotgun sequence.
ACCESSION   DAORPD010000644 DAORPD010000000
VERSION     DAORPD010000644.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22482_length_1256_cov_2.427977"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..728
                     /locus_tag="PK976_07530"
     CDS             <1..728
                     /locus_tag="PK976_07530"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HRC67775.1"
                     /translation="SQDFNDRTFGSWTAVSVIGDQQWTIDTIHGIGNTPCARISGYAT
                     TSNANEDWLISPKLNFANYTSEIIQFYSAKNYTGPDIQVMVSTDYSGLGNPNLAYWES
                     LDAVLSPGGWAWTFSENVDIANFASGNLYTAFKYTSTATESATWEIDDIKIWGHQEIG
                     IEQTKIHQISISPNPAREFVKISIQHPSELLLYSLTGEKLLQIHLSQGTNSLNLQDYP
                     SGIYIIKLVPEGYPTEVYKLIIR"
     gene            complement(811..>1256)
                     /locus_tag="PK976_07535"
     CDS             complement(811..>1256)
                     /locus_tag="PK976_07535"
                     /inference="COORDINATES: protein motif:HMM:NF024490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HRC67776.1"
                     /translation="QAGVSIKLLIRGICVIKPGIPNLSDNIEALSIVDRFLEHSRVFI
                     FCNGGRNKYFISSADLMPRNLDHRIEVVCPIFDKGIQKELMQMIQIELNANVKARSLH
                     DGHINEYRKVNSDQRIRSQYEKYYMFRKMLKGNHFKNKTHDRSTE"
BASE COUNT          365 a          243 c          258 g          390 t
ORIGIN      
        1 attctcaaga tttcaacgat cgtacttttg gctcttggac ggcggttagt gttataggtg
       61 atcagcaatg gaccatagat accattcatg gaattgggaa caccccttgt gctagaataa
      121 gtggatatgc tactacaagc aacgctaatg aagattggct tatcagcccg aaattgaatt
      181 ttgcaaacta cacgagcgag atcatacaat tttatagtgc gaaaaattac acaggccctg
      241 atatccaagt aatggtatct accgattatt cgggtttggg taatcccaat ctcgcttatt
      301 gggaaagtct cgatgcagtg ctctcgcctg gaggatgggc atggacattt tcagaaaacg
      361 tcgatattgc taatttcgcc tcaggtaatc tatataccgc ctttaaatac accagtacgg
      421 ctaccgaaag tgcaacttgg gaaatagatg acatcaagat atggggtcat caggagatag
      481 gaatagagca gactaaaatt caccaaatta gcatttcgcc taaccctgct agagaatttg
      541 taaaaatatc aatacaacat ccttccgaac ttcttttata ttctcttaca ggagaaaaat
      601 tactgcaaat ccatttatct cagggaacaa actctctgaa tttgcaagat tatccatcgg
      661 gcatttacat tattaaactt gttccggaag gttatccaac cgaagtatat aaattaatca
      721 ttcgataaaa tatttgaagg gaagccgatc tttgtgataa actaggtcgg tttttctttc
      781 tggtacgata cgtgcgtaat aatggctgtt ttattctgta ctgcggtcgt gtgtcttatt
      841 tttaaaatgg ttccctttga gcatttttcg aaacatataa tatttttcgt actgcgatct
      901 tattctctga tcggaattta ctttgcggta ttcgttaata tgtccatcat gcaatgatcg
      961 agcttttaca ttggcgttca gttcgatctg gatcatttgc atgagttcct tttgaatgcc
     1021 tttgtcgaag atggggcaaa ccacttcgat acgatggtcg aggttacggg gcatcaggtc
     1081 ggctgagctg atgaagtatt tgttgcggcc accattgcag aaaataaata cgcgtgaatg
     1141 ttccagaaaa cgatctacaa tgctcagagc ttcgatgtta tcgctcaaat ttgggatacc
     1201 gggtttgatc acacaaatac ctcttattaa aagtttgata ctgacacctg cctgac
//
LOCUS       DAORPD010000645         1255 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_22519_length_1255_cov_1.751667, whole genome shotgun sequence.
ACCESSION   DAORPD010000645 DAORPD010000000
VERSION     DAORPD010000645.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22519_length_1255_cov_1.751667"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1255)
                     /locus_tag="PK976_07540"
     CDS             complement(<1..>1255)
                     /locus_tag="PK976_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67777.1"
                     /translation="ATLSMPVRFPKFSAYSRLSYRRNVYKTTTYNIASITLSSYIKKL
                     NASISAYANWMDGDDPFIYANIGLGLPQWKGFTSRLQSQINFSDNRVISLRAEIEKRI
                     LRQGYLSLMGEENFQSKYRAISLSFRWTVPFSQVNLATRLSNNEFMITEGAQGSFAFG
                     SGKGYVHAEDRPSVGMGGLSIISFIDLNHNYKRDSGERLVKGLAMRINGGRVIQGEKD
                     SIIRVVGLIPYTDYILTIDEKSLEEISYRIAHKVIRIYAEPNQFKKIEIPVLPMGEIN
                     GWVLRKDGTGQRGEGHIIIDIYSSIGKKIASTITERDGAFSYLGLPPGHYIARLDSVQ
                     VAKLGIVSSPSEIPFEIKPDVLGDVVYDLNFFVERPPTEDKKITETPKDTLKVTEIRI
                     PLVQEFYTLQTGAFKHKHNAEHLSET"
BASE COUNT          345 a          262 c          266 g          382 t
ORIGIN      
        1 ggtttcggaa agatgttcgg cattatgctt atgtttgaaa gcccccgttt gaagagtgta
       61 aaactcttga acaagcggta ttcttatttc tgtaactttt agagtgtctt ttggggtttc
      121 ggtaattttt ttgtcctctg ttggaggtct ttcgacgaaa aagttcaaat cgtaaaccac
      181 atcgcccaga acgtcgggtt tgatttcgaa aggtatttcg gaaggagatg acactatgcc
      241 aagttttgcc acttgaaccg aatctaagcg ggctatgtaa tggccaggag gtaagccaag
      301 ataactaaat gcgccatctc tttcggtaat cgtagaagct atctttttcc ctatggacga
      361 ataaatatcg atgatgatgt gtccttcgcc tctttggccg gtaccgtctt tcctcaatac
      421 ccagccgttt atttctccca tgggcagtac aggaatctct atttttttga attgattagg
      481 ctcggcataa atgcgaatga ctttatgcgc aatacgataa gaaatctctt cgagactttt
      541 ttcgtctatg gtaaggatat agtcggtata gggaattaaa cccaccacac ggataataga
      601 atctttttcc ccctgtatga cacgaccgcc attgatgcgc atggcaagcc ccttaaccaa
      661 gcgttcgcca ctgtctcttt tgtaattgtg attgaggtct ataaaagaaa tgatggataa
      721 tcctcccatt cctacggaag gacgatcttc ggcatgcaca tagcctttcc cgcttccaaa
      781 ggcaaaactg ccctgagctc cttcggtaat cataaattcg ttgttcgaca agcgagtagc
      841 taaatttacc tgcgaaaaag gaacagtcca tcgaaaagaa aggctaattg cacgatattt
      901 cgattggaaa ttttcctcgc ccattaggga aaggtatccc tgccgcaata tccttttctc
      961 tatttcggct ctgagagaaa taaccctatt gtcgctaaaa ttgatttgcg attgtaagcg
     1021 ggaggtaaat cctttccatt gaggcaaacc gagtccgata tttgcataaa tgaatggatc
     1081 gtctccatcc atccaattgg catacgcact tatactggca ttcagttttt ttatatatga
     1141 cgaaagtgta atactggcaa tgttgtaagt agttgtttta tatacatttc gcctatacga
     1201 tagacgagaa taagcactga attttgggaa acgaaccggc atcgaaagag tggct
//
LOCUS       DAORPD010000646         1254 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_22536_length_1254_cov_2.180150, whole genome shotgun sequence.
ACCESSION   DAORPD010000646 DAORPD010000000
VERSION     DAORPD010000646.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22536_length_1254_cov_2.180150"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(435..>1254)
                     /locus_tag="PK976_07545"
     CDS             complement(435..>1254)
                     /locus_tag="PK976_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HRC67778.1"
                     /translation="GVHRLYQMAEQGKLLVPAINVNDSVTKSKFDNLYGCRESLADGI
                     KRATDVMIAGKVVVVCGYGDVGKGCAHSMRSFGARVLITEIDPICALQAAMEGFQVTT
                     VEDALAQGNIFITATGNRDIITLEHMEHMKDQVIVCNIGHFDNEIQVDRLVARPGIQH
                     INIKPQVDQFIFPDGHSIILLAEGRLVNLGCATGHPSFVMSNSFTNQTLAQIELWQKK
                     LELKVYRLPKHLDEEVARLHLNQLGAKLTRLTPEQAAYIGVNIDGPYKADHYHY"
BASE COUNT          366 a          253 c          265 g          370 t
ORIGIN      
        1 ttcaaaactg cgacaacctg attaacaccg aatcagaagt tctgagtata aagaatttgt
       61 tccccttgag ttcttaataa aactttcctt gggttgaatg atggggttat aaaaaacaac
      121 tttggaagga gaaactacaa cgattatccg acttccgcaa agtccaaaac gaacccaggg
      181 ggtgatgcaa gttcagattt tacagctgtt gaatatgaat ttttgttgcg acatggatga
      241 ggcctcgaag gcagcattta agctaaaccg gaatcaaatt tatttgaatg atgtggagcg
      301 caactgcaac actcaaatta cttttttgta tcaagcctaa atgcattata ggtttgatgc
      361 aaagtccata caaatcaatt tagtatagtt tgtaaacaaa gcaaatttat atcgatgtgc
      421 ctcatttaaa aatcttagta gtgataatgg tcagctttat aaggtccatc gatgttaacg
      481 ccgatgtagg ctgcttgttc gggggtcagg cgtgtgagtt tggctccgag ttggttgaga
      541 tgcaaacggg caacttcttc gtcgagatgt ttaggaagtc ggtaaacttt tagttcgagt
      601 tttttctgcc acagttctat ttgagccagg gtttggttag taaaagagtt gctcattacg
      661 aaagaaggat ggccggtagc acagcccaaa ttgactagac gtccttcggc cagaagaatg
      721 atggaatgtc catcagggaa gataaactgg tcaacctgag gtttaatatt gatatgttgg
      781 ataccaggtc gtgcaactaa tctatctact tgaatttcat tgtcgaaatg cccaatattg
      841 catacaatta cctgatcctt catgtgttcc atgtgctcga gggttatgat atcgcgattg
      901 ccagtagcag taataaatat gtttccctgg gccaatgcat cttcaactgt agttacctga
      961 aaaccttcca tagcagcttg taaggcacag atggggtcaa tttcggtaat cagtactctt
     1021 gctccaaagc ttcgcatgga atgggcacaa cctttcccca catcaccata tccgcaaacc
     1081 acaacgactt ttcccgctat catcacatca gtagcacgtt ttatgccatc ggctaaagat
     1141 tctctacagc cataaagatt gtcgaatttt gatttcgtaa cactatcgtt cacattaatt
     1201 gcaggcacca aaagttttcc ctgttcggcc atttgataaa ggcggtgaac cccc
//
LOCUS       DAORPD010000647         1254 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_22541_length_1254_cov_1.878232, whole genome shotgun sequence.
ACCESSION   DAORPD010000647 DAORPD010000000
VERSION     DAORPD010000647.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22541_length_1254_cov_1.878232"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..>1254)
                     /gene="gldG"
                     /locus_tag="PK976_07550"
     CDS             complement(5..>1254)
                     /gene="gldG"
                     /locus_tag="PK976_07550"
                     /inference="COORDINATES: protein motif:HMM:TIGR03521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     substrate-binding protein GldG"
                     /protein_id="HRC67779.1"
                     /translation="KGNKTLVNLLKNLPGNSGEQNINISIENLEFEITDAIRRLTSTQ
                     VNKIAFLEGHGELSEAETYDISKALSRYFQIDRGIIGTDASILDDYKVLIVAKPMFPF
                     SEKDKFIIDQYIMKGGRILWLIDGVQLLKENLSTVGFSPAWALDINLNDQLFRYGVRV
                     NPVLLQDVQCVLVPVNVASQNQSPHFEPMPWYFAPLLLTSYQHPITRNITEIKTDFCS
                     SVDAVGDNPHTKSTVLLATSNNTHIIPTPSTIDLRALPKANDKEYFNLGYVPVAILVE
                     GSFESVFRNRMLPAGLININKVQYQSKPTRQIFVADGDIIRNETSGLASDSTTLPLGF
                     DRYMNRQFGNKDFIQNAVLYLADEEGWMQLRTKTFKLRLLNKNAVRDKKNICQVINIS
                     LPIILLALFGIIYQWIRERKYSE"
BASE COUNT          406 a          229 c          196 g          423 t
ORIGIN      
        1 ccctttattc gctatatttt ctttccctta tccactgata gataattcca aaaagagcta
       61 ataaaattat tggaagtgag atattgatta cttgacaaat atttttttta tcacgtacag
      121 catttttatt tagtaaacgc aacttaaaag ttttggtacg taattgcatc caaccttcct
      181 catcggctaa atataacact gcgttttgaa taaaatcttt attaccaaat tgccgattca
      241 tatatctgtc aaaacctaag ggtaatgttg tactatcgct tgccagccca gaagtttcgt
      301 ttctaatgat gtccccatct gctacaaaaa tctgacgagt tggtttgctt tgatattgaa
      361 ccttatttat atttatcaaa cccgcaggaa gcattctgtt cctgaaaacc gattcaaaac
      421 ttccttccac cagaatagct actggcacat accctaaatt gaaatattcc ttatcattgg
      481 cttttggtaa tgcccttaaa tcaatggtag aaggtgtagg aataatatga gtattattag
      541 aagtagcaag caataccgtt gattttgtat gtggattgtc acctactgcg tcaacactag
      601 aacaaaaatc tgttttaatt tctgttatat ttctggtaat aggatgttga tatgaagtaa
      661 gaagcaaagg tgcaaaatac caaggcattg gttcgaagtg aggagattgg ttttgagaag
      721 caacatttac aggcactaag acacattgta catcttgcaa tagaacagga ttgacacgaa
      781 caccatatct aaatagttga tcgtttaaat ttatatccaa tgcccatgca ggagaaaatc
      841 caacagttga aagattttct tttaaaagtt gcactccgtc aatcaaccaa agaatacgac
      901 ctcctttcat aatgtattga tctataatga atttatcttt ttcactgaaa ggaaacattg
      961 gctttgcaac aatcaaaact ttataatcat ctaatatcga agcatctgtg ccaattatac
     1021 ctcgatcaat ttgaaaatat cgacttaaag ctttactgat atcataagtt tctgcttctg
     1081 agagttcgcc atgaccctcg agaaaagcaa ttttatttac ttgggtactt gtcaatcggc
     1141 gaatagcatc tgtaatttca aattcgagat tttctatgga aatattgatg ttctgctcac
     1201 cagaattacc tggtaaattc ttcaataaat ttaccaatgt tttattccct ttat
//
LOCUS       DAORPD010000648         1251 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_22646_length_1251_cov_1.941472, whole genome shotgun sequence.
ACCESSION   DAORPD010000648 DAORPD010000000
VERSION     DAORPD010000648.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22646_length_1251_cov_1.941472"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..>1251)
                     /locus_tag="PK976_07555"
     CDS             complement(118..>1251)
                     /locus_tag="PK976_07555"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRC67780.1"
                     /translation="QNFGSDIIYTLPGMLNNAELKPESTNGWEIGTELRFLNNRIGLD
                     FTYYYKKTTDQIFNTPISATSGFTSAMINAGSISNKGIEFLLTLTPVKVKDFTWDILL
                     NFAKNTNKVEELIEGVETLRLANGPFNISVEARVGEAYGQLVGTDFVYDAKGNKVVGT
                     NGRYLKTTVVQSLGSVLPDWNAGITNAFKFKGFDFSALIDIQHGGKIFSTSYMWGIYS
                     GMLEETAAINANGKNIRDAVADGGGVLIDGVYGKLNADGSVTYLDKDGNPVDSPVKNE
                     TYISGVRWAADHYSGPAAQNVFDASYVKLRELRLGYTIPNRYTGPIQNLRISIYGRNL
                     AIWGRDNKHIDPEYTTSSGNTQGIEGGQLPSLRSFGINLTCNF"
BASE COUNT          355 a          263 c          270 g          363 t
ORIGIN      
        1 ttgatttctt cgaaatcctt tgtacaacct gcgagaatga ataaaggcag gttgaaaaca
       61 acggcaattt tatgtaatgt tttcataatc atttgaattt aaatttttta gaaacagtta
      121 gaaattgcat gttaggttaa ttccaaagga gcgaagcgag gggagttgcc caccttcaat
      181 gccttgagta ttacctgagc tggtagtata ttccggatct atgtgtttat tgtctctgcc
      241 ccaaatggcg aggttgcgac catagatcga aattcgaaga ttctgtatgg gccccgtgta
      301 tctgttagga atagtgtaac ctaagcgcag ttcacggagt tttacataag aggcatcgaa
      361 aacattctgt gcagcaggac ccgagtaatg gtcagcagcc cagcgtacgc cagagatata
      421 ggtttcgttc tttacaggag aatccacagg gttgccatct ttatcgagat aagtaacgct
      481 tccgtcggca ttgagttttc cataaacccc gtctatcaga accccaccgc catcggcaac
      541 cgcatcgcgg atgtttttcc cattggcatt gatagcagcc gtttcttcga gcataccact
      601 ataaataccc cacatgtatg aggtggagaa tattttgcca ccatgctgga tgtctatcaa
      661 agcgctgaag tcgaagcctt tgaatttgaa agcgttggtt atgccagcat tccaatcggg
      721 gagaacgctt cccagagatt gaactaccgt agttttaaga taacgtccat tggttccaac
      781 caccttattt ccctttgcat catagacaaa atcagtacct acaagctgtc cataagcttc
      841 acctacgcga gcttcaactg aaatgttgaa gggcccattg gctaaacgaa gggtttcgac
      901 accttcgatg agttcttcaa ccttgttggt gttcttggca aaatttaaaa gaatatccca
      961 tgtaaaatct ttcaccttta caggggtaag cgtgagtaag aattctatcc ctttgttgga
     1021 aatactgcca gcattaatca tagcggaggt aaaaccactg gtagcagaaa tgggagtatt
     1081 aaatatctgg tcggtagttt tcttgtaata gtaagtgaaa tcgaggccaa tacgattgtt
     1141 aaggaacctc aattcagtac caatttccca gccattcgtg ctttcaggct ttaattcagc
     1201 attgttgagc atgccgggaa gtgtgtaaat aatatcactt ccaaaattct g
//
LOCUS       DAORPD010000649         1251 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_22653_length_1251_cov_1.766722, whole genome shotgun sequence.
ACCESSION   DAORPD010000649 DAORPD010000000
VERSION     DAORPD010000649.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22653_length_1251_cov_1.766722"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="PK976_07560"
     CDS             <1..324
                     /locus_tag="PK976_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67781.1"
                     /translation="FLQYGPPDVINDYPFDAGLISTLSDNEINKDVGGSVPYQIWHYY
                     QIKDQRNKFFVFYNPHLIPNNYQLLHSDVKGEIYNPVWQSELHRIPFINIDAGNPSSN
                     PNPNQ"
     gene            358..>1251
                     /locus_tag="PK976_07565"
     CDS             358..>1251
                     /locus_tag="PK976_07565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRC67782.1"
                     /translation="MPARIAIAILNWNGKEFLVKFLPSLGQFGKLGVEIYVIDNASSD
                     GSVDFLRENFKEVNIIENSENYGFAGGYNHGLKYIDAEYYIILNSDIQLTEGWIDPMV
                     EYLDGHAEVAACQPKIRSYDEPERFEYAGASGGYIDFWGYPFCRGRLFQAIEKDYGQY
                     DDITEIFWASGACLFIRKDCFWEVGGFDEDFFAHMEEIDLCWRLKNKGYKIVVIPQSV
                     VFHVGGGTLPKKSWRKTFFNMRNNALMLIKNLPKKWLPIVLFARLFLDFAAALKFLVD
                     GGFKNFWAVFKAYFSLIRSFGR"
BASE COUNT          350 a          209 c          271 g          421 t
ORIGIN      
        1 ttcttacagt atggcccgcc cgatgtgatt aacgattatc ccttcgatgc aggattaata
       61 tctacgctat cggataatga aatcaataaa gacgttggcg ggagcgttcc ttaccagata
      121 tggcattatt accaaattaa ggatcagcgc aataaatttt ttgtatttta taaccctcat
      181 ttgattccta ataattatca actcttgcac agtgatgtca agggtgagat ctataatcct
      241 gtatggcaaa gtgagttaca tagaattccg tttataaata tcgatgcagg caatcctagt
      301 tcaaatccaa acccaaacca gtaatcgcat gagaatttta tctttaatat aaaccttatg
      361 cctgcgagga ttgctattgc tatactcaac tggaatggga aggaatttct tgtcaaattc
      421 ttgccttccc ttggtcagtt tggtaagctt ggtgttgaaa tttacgtgat cgataatgct
      481 tcttctgacg gttcggttga ttttttaaga gaaaatttca aagaagtaaa tatcatcgaa
      541 aattccgaga actatggatt tgccggaggt tacaatcacg gactcaaata catagacgca
      601 gaatattata tcattttgaa ttccgatatt cagcttaccg aaggttggat tgatccaatg
      661 gttgaatatt tagatggcca tgccgaggtt gctgcatgcc agcctaagat tcgatcttat
      721 gacgaacctg agagatttga atatgccggt gcctcgggtg gctatatcga tttttggggt
      781 tatccgtttt gtcgtggccg tttgtttcag gctatcgaga aagattatgg ccaatacgac
      841 gatattacag aaatattttg ggctagtggg gcttgtttgt ttatcagaaa agattgtttt
      901 tgggaagttg gtgggtttga tgaagatttt ttcgcacaca tggaagaaat cgatctttgc
      961 tggcgattga agaataaagg ctataaaatt gtggttatac cccagtcggt agtatttcat
     1021 gtaggaggcg gcactttgcc aaaaaaatct tggagaaaaa cttttttcaa tatgcgcaac
     1081 aatgcgttaa tgttgataaa aaatctgcct aaaaaatggc tacccatagt tttgtttgct
     1141 cgtttgtttc ttgattttgc cgctgctttg aagtttctgg tcgatggtgg ttttaaaaat
     1201 ttttgggcag tttttaaagc ctattttagc ttgattcgat cctttggccg t
//
LOCUS       DAORPD010000650         1245 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_22836_length_1245_cov_2.440336, whole genome shotgun sequence.
ACCESSION   DAORPD010000650 DAORPD010000000
VERSION     DAORPD010000650.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22836_length_1245_cov_2.440336"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1245)
                     /locus_tag="PK976_07570"
     CDS             complement(<1..>1245)
                     /locus_tag="PK976_07570"
                     /inference="COORDINATES: protein motif:HMM:NF012350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRC67783.1"
                     /translation="LKKLNVDPSAGLSEEEAKKRLEEFGPNKLQEQKKKSIFQLFVAQ
                     LNDWLIYVLFAAVIITLLMGEYIDAIIIMAVILLNAILGVVQEVRASNAIEALQRMTR
                     HKALVKREGQIREVDSEGLVPGDILILDTGRFVAADLRLIETINLQIEESALTGESVP
                     SEKEASLILDPKTPLGDRENMAFMSSLVTYGRGEGVVIATGMNTEVGKIVGIINKEEP
                     LKTPLEIRLNQLGKVLGKLAIGICIFIFIVGILQGRDLAEMFLISVSLAVASIPEGLA
                     AIVAVVLSVGVTAMSKRNAIIRRLPAVETLGSVNIICTDKTGTLTQNKMTVLQSFTLD
                     ERKDIQRGQINYSKDSTILLAKAMILSSDATWDGQMGTGDPTEIALLMFGDDVGLDRT
                     KLEKDTRRIGELAFDSERKLMST"
BASE COUNT          342 a          285 c          241 g          377 t
ORIGIN      
        1 ggttgacata agttttctct cggaatcgaa agccaattcg ccaatgcgcc gagtgtcttt
       61 ttcaagcttg gttctatcga ggccaacatc atctccaaac attagcaagg caatctcggt
      121 aggatcaccc gtgcccatct gtccgtccca ggtagcgtcg gaagaaagaa tcatagcctt
      181 ggcaagcagt atagtcgaat cttttgaata attgatctgt cccctttgga tgtctttcct
      241 ttcgtcaagg gtaaacgatt gaagaaccgt cattttgttt tgagtaagtg tacctgtttt
      301 gtcagtacag atgatattga cagaaccaag ggtttcaact gcaggcaatc ttcgaataat
      361 ggcattacgt ttcgacattg ccgtaactcc aactgagaga acaactgcta ctatggctgc
      421 aagtccttcg ggaatggagg ccacggccaa tgaaacagaa atcagaaaca tctcggcaag
      481 atcgcggcct tgtaaaatgc cgacaataaa aataaaaata catattccta ttgctaattt
      541 tccaagcact tttccaagct ggttcaagcg aatttcgagg ggtgttttga ggggttcttc
      601 tttattgatg attccgacaa tctttccaac ttcggtattc attccagtag ctataacaac
      661 gccttctcca cgaccataag ttaccagact gctcatgaat gccatgtttt cgcgatcgcc
      721 taatggcgtt ttaggatcga gtataagaga agcctctttt tcggagggaa ccgattctcc
      781 tgttaatgcc gattcttcga tttgaagatt gatggtttca attaaccgaa ggtcggctgc
      841 cacaaatcga ccagtgtcta aaatcaagat atcgcccgga accaaaccct cagaatcaac
      901 ttccctgatt tgtccctctc tttttactaa ggccttgtga cgggtcattc tctgcaaggc
      961 ttcgatggcg ttacttgccc taacttcctg aacaactcca agaatggcat ttagtaaaat
     1021 cactgccatg attataattg cgtcgatata ttcacccatc aataaggtga tgataacggc
     1081 agcaaataat acatagatca accaatcatt taattgagct acaaataatt gaaaaatgct
     1141 ttttttcttt tgctcctgaa gtttatttgg cccaaattct tccagccttt tttttgcctc
     1201 ttcttcgctt aatcccgccg aaggatctac atttaatttt ttcag
//
LOCUS       DAORPD010000651         1245 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_22847_length_1245_cov_2.040336, whole genome shotgun sequence.
ACCESSION   DAORPD010000651 DAORPD010000000
VERSION     DAORPD010000651.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22847_length_1245_cov_2.040336"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="PK976_07575"
     CDS             <1..269
                     /locus_tag="PK976_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008620231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HRC67784.1"
                     /translation="EGFSRLKAFNYGQLSGLAEPIAGLIGVYLVLAITPILPYALSFA
                     AGAMIYVVVEELIPESQIGDETDLSTVGTIIGFALMMLLDVAFG"
     gene            complement(397..>1245)
                     /locus_tag="PK976_07580"
     CDS             complement(397..>1245)
                     /locus_tag="PK976_07580"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HRC67785.1"
                     /translation="GMSKYWEYVKGNIEFDPVTGYGSNRMPLWVKPQKKVRVRDMMDF
                     MRDHLEGTELDMSKDLGAGPYGNPYRWRPLTFEVDGQIYLNERATATQQTGFSFVSQM
                     RSWLPREIGGIFWFSVDDADNTVYYPVYCSSTRAADKYARGYGSMMEFRDDAAFWVFN
                     QVSNFVYTRYRDIHPEVRVMQDKLENFYIDFTPTIDSYALKLYGTDKSKAIDFLTDYS
                     VNIGNNLVMQWKNFYAYLFTKYMDGNIKVKDGNKKNPKVSQPGYGEQWYRYIVEKTGD
                     KLKYKQ"
BASE COUNT          342 a          257 c          258 g          388 t
ORIGIN      
        1 gggaaggttt ttcgagatta aaagccttta actatggcca gctttctggt cttgctgaac
       61 ccatagccgg attaatcggt gtctatttag ttttagctat cactcctatt ttgccctatg
      121 ccttatcgtt tgctgctggt gcgatgatct atgtggtagt ggaagaattg attcccgaat
      181 cgcaaattgg agatgagacc gatctttcga ctgtaggtac aattataggt tttgcgttga
      241 tgatgctttt ggatgtagct tttggataag gtaaaagaag taagatttag gaaattcata
      301 caggatcggc cggagtggag gataatgtag gaggtggcct acggctacct cctacattat
      361 ttctaaagac aattttatcg aacaaaagcg aatgatttat tgcttgtact tcagtttgtc
      421 gccggttttt tcaacgatat aacgatacca ttgctcacca tagccaggtt gactgacttt
      481 gggattcttc ttattgccat cttttacttt aatatttcca tccatatatt tagtaaacaa
      541 ataggcataa aaattcttcc attgcattac aaggttattc cctatattaa cactgtaatc
      601 ggtgaggaaa tcaatagcct tcgatttgtc ggttccgtat agtttcaggg cataagaatc
      661 tatagtgggg gtaaaatcaa tatagaaatt ttcgagctta tcctgcatga cccttacttc
      721 agggtgtata tcgcgataac gggtatagac gaagttactt acttgattga atacccagaa
      781 ggctgcatca tctctaaatt ccatcatact tccatatccg cgggcatatt tatcggctgc
      841 acgagtagac gagcaataaa caggataata aacggtattg tcggcgtcgt cgacactgaa
      901 ccagaatatt ccaccgatct cacgaggaag ccatgaacgc atttgcgaaa cgaaagagaa
      961 gcctgtctgc tgtgtcgcag tagctctttc gttcaaataa atttggccat ctacctcaaa
     1021 agttaatgga cgccatcggt aaggattgcc ataagggccc gccccaaggt ctttgctcat
     1081 atcgagctcg gtgccttcca gatggtcgcg cataaaatcc atcatatctc ttactctgac
     1141 ctttttttgg ggtttaaccc aaagaggcat acgattgctc ccataacccg taactggatc
     1201 gaattctata ttgcctttaa catattccca atatttactc atgcc
//
LOCUS       DAORPD010000652         1244 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_22884_length_1244_cov_2.091674, whole genome shotgun sequence.
ACCESSION   DAORPD010000652 DAORPD010000000
VERSION     DAORPD010000652.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22884_length_1244_cov_2.091674"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1244)
                     /locus_tag="PK976_07585"
     CDS             complement(<1..>1244)
                     /locus_tag="PK976_07585"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRC67786.1"
                     /translation="SSVGVDIALSPKQGHYAFQIKACRIPGNNFYDEGHTWAALGEQD
                     GINYSLGKNGWIILDMYDTIYNGNGNDILVVEGDSSPESYSVYAAITQDGPWKLMGSA
                     SGTHEFDLTSANLNKARYLKIVDGGTGSYFGEDAGFDLDGVKVLYIPVSANFTASNTM
                     PCIDNSIDFADNSLGNPTIWQWLFQGGNPSSSNSQNPSGIMYPNPGIYDVTLTVGDGV
                     TSSSLTKSGYIHVYGTPLPPLTPLGDSSFCQGLSQSDYTTAGAPNATSYEWVLTPTDA
                     GIITGNWIIATVNWNPNFNGQAMVKARETSPCGTGQWSLPKVVDVMERPIVDLGNDTI
                     IAASASLLLDAGNPGCSYLWNTNETTQTIIADTNGIGAGIHEYWVRVTSPNLCSSSDT
                     ILINFSIDIGMPDFSVSSIKVFP"
BASE COUNT          344 a          284 c          272 g          344 t
ORIGIN      
        1 ggaaagactt taattgaaga aaccgaaaaa tcaggcattc ctatgtcgat gctgaagttg
       61 ataagaattg tatcggagct cgaacaaaga tttggagaag ttactcgaac ccaatattca
      121 tgaattcccg ctccaatgcc attggtatcg gcaataatag tttgggtagt ttcatttgta
      181 ttccatagat atgagcatcc cggattgcct gcatcgagca acagagaagc tgaagctgca
      241 ataatggtat cgtttccgag atcgactatg ggtctttcca tcacatcgac tactttaggt
      301 aatgaccatt ggccagtacc gcaaggactt gtctcccttg cttttaccat tgcctgacca
      361 ttgaaattgg gattccagtt tacggtagcg atgatccagt tgcccgtaat gatgcctgca
      421 tcggttgggg tcagcaccca ttcataagag gtggcattag gtgctccagc ggtagtgtaa
      481 tcactttgtg aaagaccctg acaaaatgag ctatctccca gaggagtcaa tggaggcaag
      541 ggcgttccat aaacatggat atatcctgat tttgtaaggg aagaggaagt aacgccatca
      601 cctacggtga gggttacgtc atagattccc ggatttggat acatgattcc tgatggattt
      661 tgggagttag aggaagatgg atttccgcct tggaagagcc actgccagat cgttgggtta
      721 cccaaagaat tatcggcgaa atcgatgcta ttgtcaatgc acggcatggt attggaagcg
      781 gtaaaattgg cagaaaccgg aatgtatagc acttttactc catcaaggtc gaaaccggca
      841 tcttcgccaa aatacgaacc cgtcccacca tccacaatct ttagatacct tgctttgttc
      901 aggttggctg aagttagatc gaattcatga gttccgctag cactgcccat caatttccaa
      961 ggcccatcct gagttatagc tgcatacaca ctatacgatt cggggcttga atctccttct
     1021 accactaata tatcgttgcc attgccatta taaatcgtat catacatatc caaaataatc
     1081 caaccattct tcccaaggga atagtttatt ccatcctgtt ctccgagagc cgcccaggta
     1141 tgtccttcat cgtagaaatt attcccggga atcctgcaag cctttatctg aaatgcataa
     1201 tggccttgct ttggactcaa tgctatatct acccccaccg aact
//
LOCUS       DAORPD010000653         1241 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_23010_length_1241_cov_1.984823, whole genome shotgun sequence.
ACCESSION   DAORPD010000653 DAORPD010000000
VERSION     DAORPD010000653.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23010_length_1241_cov_1.984823"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..448)
                     /locus_tag="PK976_07590"
     CDS             complement(29..448)
                     /locus_tag="PK976_07590"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HRC67787.1"
                     /translation="MTIAIINGPNLNLLGKRQPHIYGSERFEDYLRQLETTYPYINFI
                     YRQHNMEGEIINTLHEFGFSTDGIILNPGGFTHTSVAIADAIRSIYCPVVEVHISMLF
                     GREPYRQKSLTASACKGFICGFGLESYRLAVESFLQK"
     regulatory      532..630
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            715..>1241
                     /locus_tag="PK976_07595"
     CDS             715..>1241
                     /locus_tag="PK976_07595"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRC67788.1"
                     /translation="MVLVPYVAMTQYILSGTVIDATSGQRLAGANVVILNSPYGAVTN
                     DAGYFSIKKLPWGNYPLKISYVGYKSYIDTIKIDRNLYLKIELQTVAHMQEEVMVKAT
                     RVDISVPTTFSELHRKEIRKLNTGRDLPYVLQMSPSVVASSDAGTGIGYTSLRIRGTD
                     MTRINVMVNGIPMKDT"
BASE COUNT          384 a          215 c          255 g          387 t
ORIGIN      
        1 taattctcta ttagagagat gtttattttt atttttggag aaagctttct acagctaacc
       61 gatagctttc taatccaaag ccacaaataa aacctttgca ggccgaagca gtaagcgatt
      121 tttgtcggta aggttcacgg ccaaagagca tgctgatgtg cacttctacc acagggcagt
      181 aaatagaacg tatagcatct gctattgcta ccgaagtatg tgtaaaaccg ccagggttta
      241 gtataatccc atcggtggaa aatccaaatt catgtaaggt attgatgatt tcaccctcca
      301 tattatgttg tcgatagatg aaattgatat aaggataagt tgtttcgagc tgacggagat
      361 agtcttcgaa tcgttcagaa ccatagatat gaggttgtcg ttttcctaaa aggtttagat
      421 ttggtccatt gataatcgcg atagtcatct tttatcaaat catttttcat caaaaatagc
      481 aaattatttt atcaaagtgt ttattagata aaatattcta attttgccgt agatacctta
      541 taggggtgtc ccgctataac ggggctgaga tcataccctt tgaacctgat ccggatcata
      601 ccggcgtagg gaaatataat tcattcatga ccttttcccc tatatggttt taatacattg
      661 ttaaccaatt aaaaccatta ttttatgttc aaaaaaactt caattttcat tttcatggtg
      721 cttgtcccct atgtggccat gactcagtat atattgagtg ggacggttat tgatgccacg
      781 agtgggcaaa ggctcgcggg cgccaatgtt gttatattga attctcctta tggagccgta
      841 accaacgatg ccggatattt ttccattaaa aaacttcctt ggggaaatta tcctttaaaa
      901 atcagttatg tgggatataa gtcttatatt gataccatta aaatagacag gaatctctat
      961 ttaaagatag aattacagac tgtagctcac atgcaggaag aggtaatggt gaaggccaca
     1021 agggtagata tttcggttcc cactactttt agcgaactcc atcggaagga gataaggaaa
     1081 ttgaacactg gacgagattt accttatgtg ttgcaaatga gcccttcagt ggtagcaagt
     1141 tccgatgcag gtacagggat tggatatacc agtttaagaa ttcggggcac agatatgact
     1201 cgaataaatg taatggtaaa tggaattcca atgaaagata c
//
LOCUS       DAORPD010000654         1238 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_23101_length_1238_cov_2.304311, whole genome shotgun sequence.
ACCESSION   DAORPD010000654 DAORPD010000000
VERSION     DAORPD010000654.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23101_length_1238_cov_2.304311"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /gene="lgt"
                     /locus_tag="PK976_07600"
     CDS             <1..650
                     /gene="lgt"
                     /locus_tag="PK976_07600"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HRC67789.1"
                     /translation="TLLTYVVLGAIIGARLGHCLFYEPEVYLRDPVRILKIWEGGLAS
                     HGGALGVILALWLYSCKTHQSFWWTTDRVAILVGMAGASIRLGNVMNSEIIGKPTDLP
                     WAFIFARVDKIPRHPTQLYESLAYLLIFFLMLYLYHRFLYKNRIIPPGYFVGLFLILV
                     FTVRFFIEFLKEPQVGFEQNLPLNMGQLLSIPFIIFGLWLVLRKHKTVANKIQSS"
     gene            647..>1238
                     /locus_tag="PK976_07605"
     CDS             647..>1238
                     /locus_tag="PK976_07605"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HRC67790.1"
                     /translation="MIFDLTHPIAEGMPVWPGTPSPKLEKECTIEKDGFLETHLGLSS
                     HTGTHIDAPAHILPGSYTLDKFRPEYFIGRAIKIDLTHCHHTILEEEFLFLEDFPEAF
                     DFVLLQTGWSRYWGHPAYFRNFPVLSLQSARFLSSSQIKGIGLDAISFDLAESNTFPN
                     HHIFMGKGILLIENLTGLEPLPLGKSFELFALPLYFR"
BASE COUNT          313 a          259 c          269 g          397 t
ORIGIN      
        1 atactctgtt gacttatgta gtcttgggtg caattatcgg ggcgcgtttg gggcattgtt
       61 tgttctatga gcccgaggtt tatcttcgcg atcctgttag aatccttaaa atatgggaag
      121 gaggcttagc cagtcatggc ggtgcacttg gagtcattct cgccttatgg ctttattcct
      181 gtaaaacaca tcagagtttc tggtggacta ccgatcgtgt ggctatttta gtcggcatgg
      241 caggagcgag catacgtttg ggtaatgtca tgaattccga gataataggc aaacccaccg
      301 acttaccttg ggctttcatt tttgcgagag tggataaaat accccgacac cccacccagt
      361 tatacgaatc tttggcttat ctgctgatct tcttcttgat gttgtatctc tatcatcgtt
      421 tcctgtataa aaatagaatc attccaccgg gatatttcgt cggactgttt ctaattttgg
      481 tatttacagt tcgtttcttc atagagtttt tgaaggaacc tcaggtaggc ttcgagcaga
      541 atctgccctt gaatatggga caattgctca gtatcccgtt tattatcttt ggattatggc
      601 ttgtgcttcg aaaacacaaa acggtggcaa ataaaattca atcctcatga tattcgatct
      661 tactcatcca attgcagaag gtatgccggt atggccagga acgccatcac caaagctcga
      721 aaaagaatgt acaatagaga aagacggttt tctggaaacc catctcggtc tgagttcgca
      781 tacaggaacg cacatagatg cacctgctca tattttgccc gggagctaca ctttagataa
      841 gtttcggcct gaatatttca tcggcagagc aatcaaaatc gatcttacac attgccatca
      901 taccattttg gaagaagaat ttttatttct cgaagacttt cccgaagctt tcgattttgt
      961 attattacaa acggggtgga gccggtattg gggtcatccg gcctatttca ggaattttcc
     1021 ggtactttcg ctccagtcag ccaggtttct cagttcttca caaataaaag ggataggttt
     1081 ggatgccatt tctttcgatc tggctgaaag taacaccttc cctaatcatc atattttcat
     1141 gggaaaaggt atacttttga tagaaaatct cacagggctg gagcctttac ctttgggaaa
     1201 gagtttcgaa ttgtttgctc ttcctctgta ttttcgct
//
LOCUS       DAORPD010000655         1237 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_23141_length_1237_cov_1.879865, whole genome shotgun sequence.
ACCESSION   DAORPD010000655 DAORPD010000000
VERSION     DAORPD010000655.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23141_length_1237_cov_1.879865"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..797
                     /locus_tag="PK976_07610"
     CDS             <1..797
                     /locus_tag="PK976_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67791.1"
                     /translation="YDLLYLYFNPRFKSAFLSSEKQSFSTSEKALTAQILHRLLATFL
                     VLMREERESFGQLMNNTRQLANRFQNERNKTELLEKQAGQSLVEYSKDILNELACKYN
                     RKALLSPEAETLIRNFSGKLSHLKELLTNAFHFSLILNEPNQELMIQDWHMDIEKSPL
                     KSDKRRKEKYEHLGRSGDLLNRLEFAARSVLNDRLPLTGKNVGSHCDKPISAPAISDV
                     LKKHRSEIIALFEQYPDQWPIIRKDFAPIRNILIASGEKGKNLRVG"
     gene            complement(967..>1237)
                     /locus_tag="PK976_07615"
     CDS             complement(967..>1237)
                     /locus_tag="PK976_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67792.1"
                     /translation="GYIENSIKVLETIADEISTNVYISLMSQYNPQFYQKMDVALQRP
                     IDLQEYEMVVQRFYELGFHNGWIQELESHKTYNPDFEQEHPFEMS"
BASE COUNT          390 a          242 c          237 g          368 t
ORIGIN      
        1 gatatgacct gctctatttg tattttaatc caagattcaa atccgccttt ttatcttctg
       61 aaaaacaatc ttttagcact agcgagaaag cacttactgc acaaatcctc catcgtttgt
      121 tagcaacatt tttagtattg atgagagaag aaagggaatc attcgggcaa ttgatgaata
      181 atactcgtca gttggccaat agatttcaga atgagcgaaa taagacagaa ctgcttgaaa
      241 agcaggctgg gcagagcctc gtggaatatt cgaaggatat attgaatgaa ttagcctgca
      301 agtataatag gaaagcctta ctttctcctg aagccgagac attaatcagg aatttttccg
      361 gaaagctttc tcacctcaaa gaattattaa ccaacgcatt tcatttttca ctgattttaa
      421 atgagcctaa tcaggagcta atgattcagg attggcacat ggatatagaa aagtcgccat
      481 taaaatccga taagcgaaga aaggaaaaat atgaacattt gggacgctcg ggtgatttgc
      541 tgaatcgttt ggaatttgct gctcgaagcg tgttgaatga tcgattgcct ctaacgggga
      601 aaaatgttgg cagccattgt gataaaccca tatcggctcc ggctataagc gatgtattaa
      661 aaaaacaccg ttcagaaatc atcgccttat tcgagcaata tcctgatcaa tggcccatca
      721 tacgcaaaga ttttgctcct ataagaaaca ttctcatagc gtcgggcgag aaagggaaaa
      781 acctaagagt cgggtgaacc atatcctgtg tcaacgtaaa agtgtcaggc cataaaacgg
      841 agcagatcga atacttatca tgtattacca actcataggg ctcaagcaaa aattgacgat
      901 tttcttgaat tgacctctcg tcaatactat tctggcattg gtactcacat tctccatttt
      961 ctctactcag ctcatttcaa aaggatgttc ttgttcaaaa tctggattgt aggttttatg
     1021 actctccaat tcctgtatcc agccattatg aaatccaagc tcataaaatc gctgtactac
     1081 catttcgtat tcttgcaaat cgattggacg ttgtaaagcc acatccattt tctggtaaaa
     1141 ttgaggattg tattgtgaca tcaggcttat ataaacattc gtagaaattt cgtcggctat
     1201 ggtttcgagc acttttatac tattttcaat ataaccc
//
LOCUS       DAORPD010000656         1236 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_23167_length_1236_cov_2.418290, whole genome shotgun sequence.
ACCESSION   DAORPD010000656 DAORPD010000000
VERSION     DAORPD010000656.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23167_length_1236_cov_2.418290"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1236)
                     /locus_tag="PK976_07620"
     CDS             complement(<1..>1236)
                     /locus_tag="PK976_07620"
                     /inference="COORDINATES: protein motif:HMM:NF012999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRC67793.1"
                     /translation="TSRFDPRGNIVQSVCASCGGCQQFPTYPNPGAWSNTNHSTNCNN
                     AIFKIHLKSDIATANFIIPQSGCAPYEVQFQNTSVGLSFLWDFGDTISGAANYSSLKN
                     PSHTYHQAGLYTITLISFLPGSCNSSDTIQKNLLILNDTTMWLDSLVICPNNSVQIGI
                     SPSPDTNVCYQWIPDTCLSNPAISNPFAFPQTTTTYMCLVTHPGCTDTLLQTLTVIPF
                     IISAGEDTVSCSKWFTLQGSSESAGTLFHWSSLKNFLDTLNLSPFDSTAFIDLTSYSG
                     NTFYLKAYNSIGCESIDSVKVIYGGASISTSSDRLACYNDTLHLHAYNLYPNNILTWA
                     WQPEAYILTGAHSPDPIVKPDTSTWFFVSASSLYDCQTTDSIYIQVSHILADLSVHPT
                     SCYDTCDGWAKVNPLGGFSP"
BASE COUNT          376 a          215 c          300 g          345 t
ORIGIN      
        1 ggagaaaatc cacctaaagg attaactttt gcccatccgt cgcaagtatc gtaacagctt
       61 gtcggatgga cggataagtc agccaatata tggctaacct gaatataaat actatccgta
      121 gtttggcaat catagagaga tgaggcactc acaaagaacc atgtgcttgt atcgggcttg
      181 actatggggt ccggactgtg agcgcctgta agaatatagg cttcgggttg ccatgcccat
      241 gttaatatat tgttaggata taaattatag gcatgtagat gtaaagtgtc gttatagcaa
      301 gctaaacggt ctgaactggt cgaaatgctt gcccctccat aaattacttt tacactatcg
      361 atagattcac atccaattga attataagct tttagataaa atgtatttcc tgagtagctg
      421 gttagatcga tgaaagcagt gctatcaaag ggagataagt tcaatgtatc gagaaaattt
      481 tttaaagatg accaatggaa tagagtacct gcactttccg atgaaccttg tagggtaaac
      541 catttggaac aacttacggt atcttcgcct gccgaaataa tgaatggaat cactgtgagg
      601 gtttgaagga gggtatcggt gcatccggga tgtgtaacca agcacatgta agttgtggta
      661 gtttgcggga atgcgaaagg attggaaata gcagggttac tgaggcaagt atcaggaatc
      721 cattgatagc atacattcgt atctggggaa ggagaaattc caatctgaac agaattattc
      781 gggcaaatca ccaagctatc gagccacatt gtagtatcgt ttagaataag caagtttttc
      841 tgaatcgtat ccgatgaatt gcaactgcct ggtaaaaagc ttatgagggt aatagtgtat
      901 aatccggctt ggtgataagt atgagatgga tttttcaaac tggagtaatt ggcggctccc
      961 gagatcgtat ctccaaaatc ccacaaaaaa gacaaaccaa cgctggtatt ttggaattga
     1021 acttcgtatg gagcgcaacc actttgaggg atgatgaaat tggctgtggc aatatcactt
     1081 ttcaaatgaa tcttaaatat ggcattgttg cagttggttg aatggtttgt attcgaccat
     1141 gcaccaggat tgggataagt tgggaattgc tgacagcctc cgcatgaggc acaaaccgat
     1201 tgaacgatgt ttcctctcgg atcgaagcgg cttgtg
//
LOCUS       DAORPD010000657         1235 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_23214_length_1235_cov_2.050000, whole genome shotgun sequence.
ACCESSION   DAORPD010000657 DAORPD010000000
VERSION     DAORPD010000657.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23214_length_1235_cov_2.050000"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /gene="atpD"
                     /locus_tag="PK976_07625"
     CDS             <1..816
                     /gene="atpD"
                     /locus_tag="PK976_07625"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005676098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HRC67794.1"
                     /translation="KSQATLVFGQMNEPPGARARVALSGLTLAEYFRDGDDQSGGRDI
                     LFFIDNIFRFTQAGSEVSALLGRMPSAVGYQPTLATEMGLLQERITSTKRGSITSVQA
                     IYVPADDLTDPAPATTFAHLDATTVLSRKIAELGIYPAVDPLESTSRILTPENVGEEH
                     YNTAQNVKRILQRYKELQDIIAILGMDELSEEDRLIVQRARKVQRFLSQPFHVAEQFT
                     GVKGTMVGIEDTIKGFNMIMNGEVDEFPEAAFNLVGTIEEAIEKGKKFVEQSK"
     gene            822..1061
                     /locus_tag="PK976_07630"
     CDS             822..1061
                     /locus_tag="PK976_07630"
                     /inference="COORDINATES: protein motif:HMM:NF014838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HRC67795.1"
                     /translation="MKVELISPQKRIFHSDDIILVQLPGIDGLFEILNRHAPMIALLK
                     KGNIKVKDSQNKVTFFRIEKGFVEVKNNVVTIFSQ"
BASE COUNT          406 a          238 c          255 g          336 t
ORIGIN      
        1 aaatcgcaag ctacattagt ttttggtcaa atgaacgaac cccctggagc acgcgctcgt
       61 gttgctcttt caggactgac cttggccgag tactttagag atggggatga ccaatcgggg
      121 ggtcgagata ttttattttt tatagataat atttttagat ttactcaggc cggaagcgaa
      181 gtttcggccc ttttaggccg catgccttca gccgtgggat accaacctac tctggcaacc
      241 gaaatgggtt tgcttcaaga aaggattacc tctacaaaaa gaggttctat aacttctgtt
      301 caggctattt acgttccggc cgacgatctt acagatcctg cacccgctac aacatttgcc
      361 caccttgatg ccacgacagt tttaagccga aaaattgccg aacttggaat ttatccggca
      421 gtagatccct tagaatccac ttctcgaatt ttaacccctg aaaatgtagg agaagaacat
      481 tataacactg cccaaaatgt aaaaagaatc cttcaacgat ataaagaatt gcaggacata
      541 attgccattc taggaatgga tgaactgtcg gaagaagata gattaatcgt tcaaagagca
      601 cgcaaagtac aaagattcct ttcgcaacct ttccacgtag cagaacaatt tacgggtgta
      661 aaaggcacta tggtgggcat tgaggatact atcaagggct tcaacatgat aatgaacggc
      721 gaagtcgatg aatttccaga agccgcattt aaccttgtgg gaacaatcga agaagcaata
      781 gaaaaaggga aaaaattcgt cgagcaaagt aaatgattct tatgaaagtt gaactaattt
      841 cgccgcagaa gagaattttt cacagtgatg atataatatt ggtacaacta ccaggaatcg
      901 atggattatt tgagatactg aatcgccatg ctcctatgat tgctcttctg aaaaaaggaa
      961 acatcaaagt gaaagatagc cagaataagg ttacattttt tagaatcgaa aaaggatttg
     1021 ttgaggtgaa gaacaacgtg gtaaccattt tttcacaatg atttttataa gtaataattg
     1081 tctccttgag aatcttcgaa aagtaataca acttatttta aatcaactct tttaataata
     1141 tatgaaacaa aaaaggctca tcattgagcc tttaccattt catcgccgaa tgagtggtgt
     1201 cggcgggaat cgaaccagcg acacacggat tttca
//
LOCUS       DAORPD010000658         1233 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_23292_length_1233_cov_2.202037, whole genome shotgun sequence.
ACCESSION   DAORPD010000658 DAORPD010000000
VERSION     DAORPD010000658.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23292_length_1233_cov_2.202037"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /gene="murF"
                     /locus_tag="PK976_07635"
     CDS             complement(<1..808)
                     /gene="murF"
                     /locus_tag="PK976_07635"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HRC67796.1"
                     /translation="MENMEELYRLYLSSKGICTDSRKLEVGQLFFALRGENFDGNDFA
                     EIALENGAIAVVIDDPEKIALRNFYYVPDALTALQQLAQFHRKHFQIPVIAITGSNGK
                     TTTKELISSVLSGKYRIKATSGNLNNHIGVPLTLLSIQADTEFAIIEMGANHLGEITF
                     LCEIVQPDYGLITNIGKAHLEGFGNLEGVIRAKTELYKFLALSNGLAFVNGKDELLLT
                     KSSNLRRILYNSDSSAVKGDIVDTTGYLKVSLRILNRDYLLHTQLVGAYNL"
BASE COUNT          397 a          207 c          221 g          408 t
ORIGIN      
        1 gaagattgta ggcaccaact aactgggtgt gcagcaaata atccctgttt aaaattctca
       61 ggctcacttt cagataacct gtggtatcta ctatatctcc ttttacagcg cttgagtcag
      121 aattgtacaa tattcgacga agattgctgc ttttggtcaa aagcagttca tccttgccgt
      181 ttacaaaagc caatccattg gatagggcta aaaatttata taattcggtt tttgccctaa
      241 taaccccttc taaattccca aatccttcca gatgtgcctt gccaatattc gtgataaggc
      301 cataatcggg ctgcactatc tcacataaaa aggtaatttc gcctaaatga tttgctccca
      361 tttcgatgat ggcaaattct gtgtcggctt gaatggataa taaagtcaat ggaaccccaa
      421 tgtgattatt taaattcccg gaggtagcct ttatccgata ttttcccgat agaacactcg
      481 aaatcaattc tttggtggtt gttttcccat tagaacccgt aatggcaatt actggaatct
      541 gaaaatgctt gcggtgaaac tgtgcaagtt gttgtaaggc ggttaaggca tcgggaacat
      601 aataaaaatt tcgaagagct attttttcgg gatcatctat aactacagca atggcaccat
      661 tttcaagagc aatttcggca aaatcgttac catcgaagtt ttctcccctc aaggcaaaaa
      721 acaattgacc cacttccaat tttctcgagt cggtacaaat acccttcgaa gacagatata
      781 agcgatagag ttcttccatg ttttccatgc cgcaaatata tttagaatac attggatgaa
      841 tatgatgttt agaataatga tgaattattt aaaagagaaa tgatattgga atgttaagct
      901 gctcaaaata aaaaaagttg atgaatttat tttaatcttc aaagaaattg tttttgttgg
      961 atttgattta ttcatttaag agtaattcat gaaaggttaa attttaaaga tgattaactc
     1021 ataaaataat gaaatttagt tacttatggt gaatttgagc tgattaacat tcggtttaag
     1081 tttgagcaat ttactggagt tatttacggg tataaaactt tccatcagga aatgttaatt
     1141 ggttagaaga aatttttcag cattcagaat tttttaagtt ctacaaacgg caccatataa
     1201 atggccgttg gaatgttttt gttaataaat aat
//
LOCUS       DAORPD010000659         1233 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_23298_length_1233_cov_1.727504, whole genome shotgun sequence.
ACCESSION   DAORPD010000659 DAORPD010000000
VERSION     DAORPD010000659.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23298_length_1233_cov_1.727504"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /locus_tag="PK976_07640"
     CDS             complement(<1..804)
                     /locus_tag="PK976_07640"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67797.1"
                     /translation="MSKETKKNRNPYIADILLIPPDQYSHQTAERVALLRREKVERPT
                     IFVGAGTCGMIAGASQTMLSIQMYLNDHLMKADVVEVGCIGLCQFEPIVDVQLPGKNR
                     ISFKNITPEKVYSLLDAVFNNTIDSVDVLGQFESRHLVPWEDVPFINRLPFFKKQKRL
                     VLENCGLINPSSIEEYLARGGYKAYAKAIQNYTSTEICEIIEQSGLRGRGGGGYATGK
                     KWMIARQTQSDQKYLICNADESDPGAFMDRALIEGDPHKLIEGIAIAAYA"
     gene            complement(808..>1233)
                     /gene="nuoE"
                     /locus_tag="PK976_07645"
     CDS             complement(808..>1233)
                     /gene="nuoE"
                     /locus_tag="PK976_07645"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HRC67798.1"
                     /translation="GAGKEALLSVLQEIQEEEGYLNAESIHKVSYCLGIPENRIYSIA
                     TFYDQFKFSPRARKTIRICEGTACHIMGSSLVAREIKKQIGIEPGQRTKDNEYELEFV
                     ECMGACSQAPAMEINDRYYNKVTPEKLRQILEHLSNFEE"
BASE COUNT          314 a          304 c          227 g          388 t
ORIGIN      
        1 tgcatatgca gctatggcaa ttccttcgat aagtttatgt ggatcgccct caataagcgc
       61 cctgtccatg aatgcgccag gatcgctttc atcggcattg caaatcagat atttctgatc
      121 actttgggtt tgacgggcaa tcatccattt tttacctgta gcataacccc cgcctccgcg
      181 tccccttaat ccactctgct cgataatctc gcaaatctcc gtagacgtat agttctgaat
      241 tgcttttgca taagccttgt atccgcccct tgccagatat tcttcaatgc tcgaaggatt
      301 gatcaaacca cagttttcca aaaccaaacg tttctgtttc ttgaaaaatg gcaagcgatt
      361 gataaaagga acatcttccc agggaactaa atgccgagat tcgaattgcc ccaaaacatc
      421 gactgaatca atagtattgt tgaatacagc gtcgagcaaa gaatagacct tctccggcgt
      481 aatattctta aaggatatcc tattcttacc tggtaattga acatcaacaa tgggttcgaa
      541 ctggcataaa cctatgcaac cgacctctac cacatcggcc ttcatcaaat gatcattcag
      601 gtacatttga atgcttagca tggtttgact tgctccagca atcattccgc atgttccagc
      661 acctacgaaa atagtcggcc tttcaacctt ttcacgcctc agcaaggcca ctctttctgc
      721 cgtttggtga ctgtattgat ctggtggaat caacaatatg tcggcaatat atgggttccg
      781 attttttttt gtttccttgc tcatggtcta ttcttcaaaa ttggataagt gttccagaat
      841 ctgacgcaat ttttcgggcg tcactttatt gtaatatcga tcgttgattt ccatggccgg
      901 ggcttggctg catgcaccca tacactccac aaactctagt tcgtactcgt tgtccttcgt
      961 cctttggccg ggttcaatac cgatttgttt tttaatctca cgggctacta aagatgaacc
     1021 cataatatgg catgctgttc cttcgcaaat tcttatcgtc tttcgtgctc gtggtgaaaa
     1081 cttgaattga tcgtaaaatg tagctatcga ataaattctg ttctcgggaa tacccaaaca
     1141 ataactgact ttatgtatag attcagcgtt caaatacccc tcttcctcct gtatttcctg
     1201 cagaactgag agtaaggctt ctttccctgc tcc
//
LOCUS       DAORPD010000660         1231 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_23349_length_1231_cov_3.123299, whole genome shotgun sequence.
ACCESSION   DAORPD010000660 DAORPD010000000
VERSION     DAORPD010000660.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23349_length_1231_cov_3.123299"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="PK976_07650"
     CDS             <1..204
                     /locus_tag="PK976_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67799.1"
                     /translation="TLRNNTQNSDANSFYQIIKLLTGTIFIIQNQNFFIFIPAGEIEF
                     LLSNIKPSKTFEEVPSPPPRCII"
     gene            324..>1231
                     /locus_tag="PK976_07655"
     CDS             324..>1231
                     /locus_tag="PK976_07655"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRC67800.1"
                     /translation="MKKLSLIILFFFNIAWLWGQKIQVIDRSTLQPIAGASVFVVHDG
                     QKIGVVTNQRGMADISGIISSNLPVHITAIGYKEQAVSVEALEHTDYKVMLIERPYDL
                     NEIVVSASRFEEKRANVPQQMMMIKQKDIKFLSQPTSAELLQSSGQVLVQKSQLGGGS
                     PILRGFEANKVLLVVDGIRMNNAIYRGGHLQNIISIDNSMLEKVEVIYGPGSVVYGSD
                     ALGGVIHFHTLKPILAENGKTLTKTHAFTRYSTAYEEKTAHVDFNIGTRRFASLSSIS
                     VSDFGDLRQGDVRNPFYGDWGKRLFYV"
BASE COUNT          368 a          242 c          243 g          378 t
ORIGIN      
        1 actctccgaa acaacaccca aaactctgat gcgaattcat tctatcaaat aattaaatta
       61 cttacgggca ctatttttat cattcaaaat caaaatttct tcatttttat tccagcagga
      121 gaaatagaat ttttgctctc caatattaaa ccttcgaaga cttttgaaga agtgccctct
      181 ccacctccac gttgtatcat ttgaatctca tacctcgatc ttatcttttt tcttatcact
      241 cttatgctct tatcacatat taaggacaaa aaaagagtga tattacaatt tatttacttt
      301 cactattaaa taacttattg aatatgaaaa aattatcttt aatcatcttg ttttttttca
      361 atattgcctg gctttgggga cagaagattc aggtcatcga tagaagtacg ctccaaccaa
      421 ttgcaggagc ctcggtattc gttgttcacg atggacaaaa gatcggtgtg gttaccaatc
      481 agagaggcat ggccgatatt tcaggaatca tttcatcgaa tcttcctgta catataacgg
      541 caataggtta taaagaacag gctgtaagcg tcgaagcact cgagcatacg gattataaag
      601 tgatgctgat cgaaaggcct tacgatttga acgagattgt ggtttcggcc agccgtttcg
      661 aggaaaaaag agccaatgtt ccccaacaga tgatgatgat caagcaaaag gatattaaat
      721 ttctttctca gcccacttcg gccgaattat tgcaaagttc tggacaagta ttggtgcaaa
      781 agagccagtt gggcggtggt agtcccatat tgaggggttt cgaggctaac aaggttcttt
      841 tggtagtaga tggcatccgc atgaataatg ctatttatcg tggaggccat ttgcaaaata
      901 tcataagcat cgacaattct atgctcgaaa aagtggaagt gatttatggc cctggctcag
      961 tagtttatgg gagtgatgca cttggcggtg tcatccattt ccatactcta aaacccatct
     1021 tagcggagaa tgggaaaact cttacaaaaa cccatgcatt tacccgatat tccaccgctt
     1081 atgaagaaaa aactgctcat gtcgatttca atatcggtac cagaagattt gcttcgctga
     1141 gttcgattag cgtttccgat tttggcgact tgcgacaggg cgacgttaga aatccatttt
     1201 atggagattg gggcaaacgt cttttttacg t
//
LOCUS       DAORPD010000661         1230 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_23399_length_1230_cov_2.279149, whole genome shotgun sequence.
ACCESSION   DAORPD010000661 DAORPD010000000
VERSION     DAORPD010000661.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23399_length_1230_cov_2.279149"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="PK976_07660"
     CDS             complement(<1..390)
                     /locus_tag="PK976_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HRC67801.1"
                     /translation="MQNTSNKTLHLISFDMPYPPNYGGIIDVFYKIRALHRVGVNIIL
                     HVFDHGKASSDELKEYCQQIYYYERKTGIDSQLSTLPYIVKSRRQPDLLKNLMKDDWP
                     IFFEGLHTTYYLSHPFLKNRLKIYRESN"
     gene            complement(371..>1230)
                     /locus_tag="PK976_07665"
     CDS             complement(371..>1230)
                     /locus_tag="PK976_07665"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRC67802.1"
                     /translation="NDLDTLLANYLIHLLRKTPLVYDSHEFFTETPEVIHRPLVRGIW
                     LTIERSILPRLEIMITVNQSLADIFHHKYKIPVVSSRNVPELKPLEVKTDRTSLSLPD
                     DKNILILQGSGINIHRGAEELIASLRFLDNVLLLIIGGGDVMPQLKIMAKNLHVNDKI
                     QFFDRMPAEELQNYTMMADIGLSIDKDICPNYHFSLPNKLFDYIHAGLPVLASPLPEI
                     RKIVEHYQVGRIIHGHEPEILANDIQSMLDDKAALNFYRHNCLKARDKLNWENEKQIL
                     IPIYAKYLE"
BASE COUNT          335 a          220 c          277 g          398 t
ORIGIN      
        1 attgctttca cgataaattt tcagacggtt tttcaagaaa ggatggctga gataataagt
       61 ggtatgtaat ccttcgaaaa agataggcca atcgtccttc atcaggttct tcaataaatc
      121 gggttggcgg cggctcttta cgatatatgg aagtgtgctg agttgggaat ctattcctgt
      181 ttttcgttca taataataaa tctgttgaca atattccttc agctcatcgc tcgatgcttt
      241 cccatgatcg aaaacatgaa ggatgatatt tacgccgact ctatgtaagg ccctgatttt
      301 atagaacacg tcgattatgc ctccataatt tggcggataa ggcatatcga aagagataag
      361 gtgaagtgtt ttattcgagg tattttgcat aaataggtat taaaatctgt ttttcatttt
      421 cccaattaag tttgtctctg gcctttaggc aattatgtct ataaaagttt agtgcagcct
      481 tgtcgtcgag catcgactga atgtcgttag ccagaatttc gggttcatga ccatgaatga
      541 ttcgtcccac ctgataatgt tcgacaattt ttcggatttc tggcagggga ctggctaaga
      601 caggcaaacc ggcatgtatg tagtcgaaca gtttattggg caggctgaag tgatagtttg
      661 gacaaatatc cttatcgatg ctcaacccaa tatcggccat catggtgtaa ttttggagtt
      721 cttcggccgg cattctgtcg aagaattgga tcttgtcatt gacgtgtaaa ttttttgcca
      781 tgatctttag ttgaggcatc acatctccgc cgccaataat aagcaaaaga acattatcga
      841 gaaatctcag cgatgcgatg agttcttcgg ctcctctgtg aatattgatg ccactgcctt
      901 gtaatataag aatattctta tcgtcgggca aactcaaaga tgttctgtcg gtcttaactt
      961 caaggggttt taattcagga acatttcttg agctaactac cggtattttg tatttatgat
     1021 ggaatatgtc ggctaatgac tgattcacgg taatcatgat ttcgagacga ggaagaatag
     1081 atcgttcgat ggttagccaa attcctctca ccaggggccg gtgaatgact tcgggtgttt
     1141 cggtgaaaaa ttcatgactg tcatatacca atggtgtctt tctgaggaga tgaattaaat
     1201 aattggctaa gagtgtatcg agatcattgg
//
LOCUS       DAORPD010000662         1227 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_23489_length_1227_cov_3.626280, whole genome shotgun sequence.
ACCESSION   DAORPD010000662 DAORPD010000000
VERSION     DAORPD010000662.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23489_length_1227_cov_3.626280"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1227)
                     /locus_tag="PK976_07670"
     CDS             complement(<1..>1227)
                     /locus_tag="PK976_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67803.1"
                     /translation="YSLTQEINYTFDFSYDHDWRLTGTTFTMNGISKRLNTLEYNELS
                     QLKAKYFNGTATAYLQRQLYHYNARGWLTSINNPYECPNDIFAMKLYYDNVPTYASSL
                     ATGCYNGNIAMIEWFTQNQNTYVNGYGFKYDGLNRLKAGRFFSRFDASAVVEPMPTYY
                     ADGIVSGIGSEKEITYDKNGNILTLNRYGHHSGGTVLFDQLSYQYNGNRLMRVIDANP
                     NSFLSGEFCNTAAPNLQNSYDANGNLTFDVDRNMLLFYNDDNLPTNISFSNWAMTNGY
                     RSDGVKMSKSIYNAEGRTVLQEEYFGNLVLNFGKPVRILHADGVIELNASLQPTFYYY
                     LKDHLGNIRAVVSPTATNLVHIDQTSEYYPFGMNISKNFTSTTINKYKYNGKEEQEMP
                     GHWLDYGARFYDPQMG"
BASE COUNT          356 a          266 c          248 g          357 t
ORIGIN      
        1 cttcccatct gggggtcgta gaaccttgcc ccgtaatcga gccaatggcc tggcatttcc
       61 tgctcttcct tgccattgta cttgtattta ttaatagtcg tgcttgtgaa attcttactt
      121 atattcatgc caaaggggta gtattcactg gtctgatcga tatgaaccaa attggtagcc
      181 gttggcgata ctaccgcccg gatattgccc aaatgatcct ttaaataata ataaaacgta
      241 ggctgaaggc tggcgttcag ttctataaca ccatcggcat gaagtatgcg cacgggctta
      301 ccaaagttca acacaagatt gccaaaatat tcctcctgca gaacagtacg gccttcggca
      361 ttataaatac tcttagacat ttttactcca tcggagcggt aaccattggt catagcccag
      421 ttcgaaaagc taatattcgt aggcaggtta tcatcgttgt aaaacaaaag catgttgcga
      481 tccacgtcga acgttaagtt accgttcgca tcataactgt tctgaagatt aggtgctgct
      541 gtattacaaa actcgccaga aaggaaacta ttgggattgg catctattac acgcatcaag
      601 cgattgccat tatattgata gctcaactgg tcgaacaaca cggtgccgcc actatgatgg
      661 ccataacgat tgagcgtgag tatatttcca tttttatcgt aagtaatctc cttttcagaa
      721 cctatgcccg agacgatgcc atcagcataa taggtaggca ttggctcaac aacagccgag
      781 gcatcaaatc tggagaaaaa tctgcctgct ttcagacggt tgaggccatc atacttaaaa
      841 ccatatccat tcacatacgt gttctggttt tgcgtaaacc attctatcat ggctatattg
      901 ccattataac agcctgtagc caaactactg gcataagtgg gcacattgtc gtagtaaagt
      961 ttcatggcaa atatatcgtt ggggcattcg taaggattat ttatggaagt aagccagcca
     1021 cgagcattgt aatggtataa ttgccgctgc aaataagccg tagccgtacc attgaaatat
     1081 tttgccttga gttgcgataa ctcattatat tccaacgtat tcaaacgttt cgatattccg
     1141 ttcatggtaa aagttgtacc cgtaagacgc cagtcatgat cgtatgaaaa gtcaaaagta
     1201 taattaattt cttgagtcaa actatat
//
LOCUS       DAORPD010000663         1227 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_23504_length_1227_cov_1.833618, whole genome shotgun sequence.
ACCESSION   DAORPD010000663 DAORPD010000000
VERSION     DAORPD010000663.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23504_length_1227_cov_1.833618"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..755
                     /locus_tag="PK976_07675"
     CDS             <1..755
                     /locus_tag="PK976_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018107827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC67804.1"
                     /translation="FFARDYTMSNIPAAWTMLFNLFPLLSLIVFLYGIYLSTIGLLGS
                     KPNRKIGILLVLFGIAGMAGYYFTSIYGIDKMTKITPQIFQQFNPMFIIILTPLTVAL
                     FGYLASKGKEPSAPRKIGIGMFLAAVGYAIMMICSIGLPAPAEISGISDKLVSPSWLI
                     GTYFVLTIAELFLSPMGLSFVSKVAPPQYSGIMQGGWLAATAIGNLLVGVVGTFWEKL
                     PLVGFWSILIVCCLLSGIFIFSIMKKLETATE"
     gene            830..>1227
                     /locus_tag="PK976_07680"
     CDS             830..>1227
                     /locus_tag="PK976_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HRC67805.1"
                     /translation="MKYPWGDDNPYYSYSTYSKRIFGGRIQKLSIKAGFTCPNRDGTK
                     GIGGCYYCNNDAFNPSYCRSDKSITQQIEEGIAFHAWRYRRATKYLAYFQAFSNTYAP
                     LSHLQKLYGEALSHPAVVGLVISTRPDCLP"
BASE COUNT          317 a          269 c          253 g          388 t
ORIGIN      
        1 ccttctttgc ccgcgattat accatgagca atattccggc cgcatggact atgctgttca
       61 atctattccc actgctatct ctaattgtat ttttatatgg catttatctt tcgaccatag
      121 gattgttggg cagcaaaccc aatagaaaaa tcggaatttt acttgttctt tttggtattg
      181 caggcatggc aggttattac tttacaagta tttatggcat agataagatg accaagataa
      241 cccctcaaat cttccagcag ttcaatccga tgtttatcat cattcttacg cctcttacag
      301 tcgctttgtt tggatatctt gccagtaaag ggaaagaacc ctcagcaccc cgaaaaatag
      361 gtattgggat gtttctggca gccgttggtt atgccattat gatgatttgc tccataggtt
      421 tgcccgcccc agccgaaatt tcgggtatat ccgataaatt agtatctccc tcatggctta
      481 tcggtactta ttttgtgcta actattgccg aattattcct tagccctatg ggactgtcgt
      541 tcgtgtcgaa agttgcacca ccccaatata gtgggatcat gcagggtggc tggctggccg
      601 ctacggccat cggaaacctg ctggtgggtg tagtgggtac cttctgggaa aaacttcctc
      661 tggttggatt ttggtcaatc ttgattgtct gttgtctcct ttcgggcatt ttcatcttct
      721 cgatcatgaa aaagctcgaa accgctacag agtaatttct tctcagtcga agaattattt
      781 accacatgaa ttctgatgta tataaaccgg aattcgaaaa gtgaatgtaa tgaaatatcc
      841 ctggggtgat gataatcctt attattctta ttcgacttat tccaaacgga tttttggagg
      901 aaggattcaa aaactctcta taaaggcagg ttttacttgc ccaaaccgtg atggaacaaa
      961 aggaatagga ggttgctatt actgtaataa cgatgcattc aacccttctt attgtcgttc
     1021 tgacaagagc atcactcagc aaatcgaaga aggaatagcc tttcatgcgt ggagataccg
     1081 gagggctacc aaatatctgg cttattttca agccttttcg aatacttatg cacctttgtc
     1141 tcatttacag aaattatacg gcgaagccct cagtcatccc gcagtagttg gtctggtaat
     1201 ctctacccgt cccgactgcc tgccaga
//
LOCUS       DAORPD010000664         1222 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_23649_length_1222_cov_2.209083, whole genome shotgun sequence.
ACCESSION   DAORPD010000664 DAORPD010000000
VERSION     DAORPD010000664.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23649_length_1222_cov_2.209083"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(33..980)
                     /gene="fmt"
                     /locus_tag="PK976_07685"
     CDS             complement(33..980)
                     /gene="fmt"
                     /locus_tag="PK976_07685"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HRC67806.1"
                     /translation="MGTPEFAVAPLKALITNGYRVEAVVTAPDKPAGRGQRMQSSPVK
                     SYAQSVGLPLFQPYFLKDPQFIAQLENLTPDIILVVAFRLIPPEIWKLTRIGAINLHA
                     SLLPDYRGAAPIQRAIMNGETITGITTFFIDEGIDKGKIILQEVIPIGETETAGSLHF
                     KMMQQGAELVLKTLDTIARGNYQLKDQGKLICEKKLNLHYAPKISKEDCKIIWHNSAK
                     NIFNQIRALNPHPGADTLLMAPDQTAQILKVYYATPIFEPLGTITPGSINTDGKSFLQ
                     VACGDGFIELTEIQLSGKKRMDIASFLRGFKLDNRWTLI"
BASE COUNT          341 a          243 c          249 g          389 t
ORIGIN      
        1 aaaacttcca accccaagac agatgagata ctttaaatca gagtccagcg gttatcgagt
       61 ttaaatcctc gtaaaaacga ggcaatatcc attctttttt taccacttaa ctgtatctcg
      121 gtcaattcga tgaaaccatc gccacacgca acttgtaaga aggatttacc atcggtattg
      181 atgcttccgg gagttatggt accaagaggt tcaaagatgg gggttgcata atagactttg
      241 aggatttggg cagtttggtc aggtgccatt agcagtgtat ctgctcctgg atgaggattc
      301 aaggctctga tttgattaaa aatattttta gccgaattgt gccagataat tttacaatct
      361 tctttgctaa ttttcggagc ataatgcagg ttcaatttct tttcgcagat gagtttgcct
      421 tgatctttaa gctgataatt tcctctggca atggtatcga gagttttcag aacaagttcg
      481 gctccttgtt gcatcatttt gaagtgcagg ctgccagcgg tttctgtttc tccaatggga
      541 ataacttcct gaaggatgat ttttcccttg tctattcctt cgtctataaa aaaagtggta
      601 atacccgtta tggtttctcc attcattatg gctctttgta taggtgccgc acctcgatag
      661 tcgggcagaa gagatgcatg aagattgata gctcctatcc gggttaattt ccatatttcg
      721 ggtggaatta atcgaaaagc cacgaccaga ataatatcag gagtcaaatt ctcgagctgt
      781 gctataaatt gtggatcttt caagaaatag ggttgaaaca agggcaagcc aaccgattgt
      841 gcataagatt ttacggggct cgactgcata cgttgacctc ttcctgctgg tttatcaggg
      901 gccgtaacaa cagcttcgac tctataacca tttgtgatta gggcctttaa cggagccaca
      961 gcaaattcgg gcgtccccat gtaaatcagt ttcaaatttt tctccatcta ccaggtataa
     1021 aaatgaaaaa agcctgatca taatttttga atcaggctca atatgaatta taaaaaatta
     1081 tctgagcata cctttggctt tggcaataaa acggtctata tcacgtgctt catagctttt
     1141 tggatagttc atccgaattt cttcataaac tttcagtgca ttctgataat cattcaggag
     1201 ttcataactc aaacctgcct tc
//
LOCUS       DAORPD010000665         1222 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_23652_length_1222_cov_2.032562, whole genome shotgun sequence.
ACCESSION   DAORPD010000665 DAORPD010000000
VERSION     DAORPD010000665.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23652_length_1222_cov_2.032562"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1222
                     /locus_tag="PK976_07690"
     CDS             <1..>1222
                     /locus_tag="PK976_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007563673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HRC67807.1"
                     /translation="GGKHFHADLSDFQPEADIEKTGKISEVLAPNQPVIVQIAKEPIS
                     TKGPRVTSEISFAGRYLVLVPFSNRISVSQKIKSQEERNRLKRLVTSIRPKNFGIIIR
                     TVAEGKLVADLDADLKSLMAKWEMVMKKLSEAKPPQKIISEIDRTSALLRDLLNESFN
                     NIYINDPGLYEETRSYIQTIAPDKVNIVKLYKGKLPIFEYFGIDKQIKNSFGRTVSIS
                     SGIYLIIERTEAFHVIDVNSGHRAKKNNDQETNALEVNLEAATEVARQLRLRDLGGII
                     VVDFIDMANPQNRKKLYEHLKEEMAKDRAKHTILPPSKFGLIQITRQRVRPETNVEVL
                     ELCPACMGTGQVKPSIILVDEIENKIRYLIHEQNEKQIKMSVHPYVYAYLTKGFPSIQ
                     LRWVFKFKKWISISA"
BASE COUNT          407 a          269 c          238 g          308 t
ORIGIN      
        1 tgggtgggaa gcattttcat gctgatttat ctgatttcca gccagaagcc gatatagaaa
       61 aaacaggaaa gatttcagaa gttcttgctc ctaatcaacc tgtcatcgtt caaattgcga
      121 aagagcccat atctacaaaa gggcctcggg ttacttccga aatttcattt gccggaaggt
      181 atctggtatt ggttccattt tccaatcgta tttctgtttc acaaaaaatt aaaagccagg
      241 aagaacgcaa ccggcttaaa cgtttggtta ccagcattcg ccccaaaaat ttcggcataa
      301 tcattcgtac cgttgccgaa ggaaagttgg tggccgacct cgatgcagac ttaaagtccc
      361 tgatggcgaa atgggaaatg gtgatgaaaa aactctccga agccaaacct ccacaaaaaa
      421 tcatcagtga gattgaccgt acatctgcct tattgcgcga tctgctcaac gaatccttca
      481 ataatattta catcaacgac cctggccttt atgaagaaac ccgtagctat atacagacaa
      541 tagctcccga taaggtgaat atcgtcaagt tatacaaggg taaattacct atattcgaat
      601 atttcggaat agataagcag ataaaaaact ctttcggccg caccgtaagc attagtagcg
      661 gtatttatct gattatcgaa cgcaccgaag cctttcatgt gatcgatgta aatagcggtc
      721 atcgagcaaa aaaaaataac gatcaggaaa ccaatgcctt ggaagtaaac ctggaagcag
      781 caacggaagt tgcccgccaa ctccgccttc gcgatctggg aggtattatt gtggttgatt
      841 tcattgatat ggccaatcca caaaaccgta aaaaattata tgaacatctc aaagaagaaa
      901 tggccaaaga cagggcaaaa cataccatac tccctccaag caaattcgga ctcatacaaa
      961 ttaccaggca gcgcgtcaga cccgaaacca atgtcgaagt actcgaactc tgccccgcct
     1021 gtatgggaac cggacaggta aaacccagca tcatcctcgt agatgaaata gaaaacaaaa
     1081 tacgttatct tattcacgaa cagaatgaaa aacaaataaa aatgagtgtt catccttatg
     1141 tatatgcgta tcttacaaaa gggttccctt ctattcaact gcgctgggtt tttaaattta
     1201 agaaatggat ttctatatct gc
//
LOCUS       DAORPD010000666         1220 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_23721_length_1220_cov_2.148498, whole genome shotgun sequence.
ACCESSION   DAORPD010000666 DAORPD010000000
VERSION     DAORPD010000666.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23721_length_1220_cov_2.148498"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            185..>1220
                     /locus_tag="PK976_07695"
     CDS             185..>1220
                     /locus_tag="PK976_07695"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HRC67808.1"
                     /translation="MKKFYYLVFLVVLVTLSKSLHSQTKELTLDAILKDRGLATKSSY
                     WITPLNDGKNYLKKENDTLKIYDYKTGAYVGYLIALKDLLDAQGEKISMEDFSLSNDG
                     KKMLIATAIEPIYRHSYYAKYYIYDLQQKKLSALSDKGQQRLAEFSPDGSKVAFVREN
                     NLFIKNLDDGSEMPLTTDGKINEIINGTTDWVYEEEFGFTKGFFWSPDGSKIAYYRFD
                     ETRVKQYCFPKYGKLYPENYCYKYPKAGEENSIVDVYVYNIEEKKALKLNTGSENDQY
                     LPRMQWAGNSGMLAIQRLNRLQNHLEILLYNPSSALGNIIYSEENKYYIEITDDLMFT
                     PDGNQFFITSQ"
BASE COUNT          405 a          225 c          225 g          365 t
ORIGIN      
        1 attcggccgc agttcttatt ctttcctcct gattcgtcat attctatttt tttattattt
       61 cgaaagttac tcccgattat aattctactt tctcatcaag aaatatagaa aataatctgt
      121 cttcattgaa aatccgcgaa aaatcaataa ttttgaccac acatatttct taaatgcaaa
      181 tatcatgaaa aaattttatt atcttgtgtt tttggtggtt ttggttacct tatcgaaatc
      241 tctacattcg caaaccaaag aacttacact cgatgctata ttgaaggacc gaggtttggc
      301 aaccaaaagt tcttactgga ttaccccttt aaacgatgga aaaaattacc taaaaaagga
      361 aaacgacact ttaaagattt atgattacaa aactggggct tatgttggct atcttatagc
      421 tctcaaagac ctgctcgatg cgcaaggcga aaaaatttcg atggaagatt ttagcctcag
      481 taacgatggc aaaaaaatgc tgattgcaac ggcaattgaa cccatttacc gacattctta
      541 ttatgcaaaa tattatattt atgatctaca gcaaaaaaaa ttgagtgctt tgtcggacaa
      601 ggggcaacag cggctggccg agttttcacc cgatggcagt aaggttgctt ttgttagaga
      661 gaacaacctg tttattaaaa atctggacga tggaagcgag atgccattga caaccgacgg
      721 aaaaatcaat gaaatcatca atggcacgac cgactgggtg tatgaggaag aatttggttt
      781 tactaaagga tttttctggt cgcccgatgg cagcaaaata gcttattatc gattcgacga
      841 aactcgtgta aaacaatatt gcttcccaaa atatggaaaa ttgtatccgg agaattactg
      901 ttataaatat cccaaggctg gcgaagaaaa ctccatcgtt gatgtctatg tttataacat
      961 tgaagaaaag aaagcattaa aattgaatac gggttcagaa aacgatcagt atttacctcg
     1021 catgcaatgg gctggaaatt caggtatgct ggccattcag cgcctcaatc gcttgcagaa
     1081 tcatctcgag attcttcttt ataatccttc tagcgcatta ggaaatatca tctatagtga
     1141 agagaacaaa tactatatag aaataaccga cgacctcatg tttactcctg atgggaatca
     1201 atttttcatc acgagtcagg
//
LOCUS       DAORPD010000667         1220 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_23741_length_1220_cov_1.638627, whole genome shotgun sequence.
ACCESSION   DAORPD010000667 DAORPD010000000
VERSION     DAORPD010000667.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23741_length_1220_cov_1.638627"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1220)
                     /locus_tag="PK976_07700"
     CDS             complement(<1..>1220)
                     /locus_tag="PK976_07700"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRC67809.1"
                     /translation="LALTATATSMVINDIQEKLQFKEKNVLSRSFYRPNLIYVVQKEG
                     DKARRLLKICSNLKGSGIVYVRNRRRTVEIAKFLVRNHHSAGVYHAGMSMKQRNNVQN
                     RWMNDELKVMVATNAFGMGIDKPNVRFVVHMDIPDSPEAYFQEAGRAGRDGKLSYAIL
                     LYDESDMSDASENFNNSWPSYGVIKNVYNALGNYYNLAIGSGKDISFDFDLNDFCNRF
                     NLKPLPTFNALKIIEKEGYIALNDALDSPSQLMFRLSGDGMYNFQVANKKFDGFIKII
                     SRMYGGALYTSHQRIIEEAIAKYIHITTDQVIQLLHELDHLEVVSYMPRKNKPQLVFV
                     IERLDLSNFYLSPENYRLRKEAAQKRLEAMLHYIKDENHCRSQILLSYFGEKTRMRCG
                     ACDYCVKHHSLPLG"
BASE COUNT          337 a          252 c          238 g          393 t
ORIGIN      
        1 tcgcccaagg gaagtgaatg gtgttttacg caataatcac atgccccaca acgcattctg
       61 gttttttctc caaaataaga taaaagaatt tggcttcggc aatgattttc gtcttttata
      121 taatggagca tggcttcgag ccttttttga gcagcttctt tccttaaacg ataattttct
      181 ggcgataggt aaaaattgga aaggtctaat ctttctataa caaaaacgag ctggggctta
      241 tttttgcgag gcatataaga gaccacttcg aggtgatcga gttcatgcaa aagctgaatc
      301 acctggtcag ttgttatatg gatgtatttt gcaatggctt cttcgatgat acgctgatgg
      361 ctggtgtata gagcccctcc atacatgcgt gaaataatct ttatgaaacc atcgaatttc
      421 ttgttggcta cctgaaaatt gtacattcca tctcccgaaa gtcgaaacat aagctgggag
      481 ggagagtcca aggcatcatt caaagctata taaccttctt tttcgatgat ttttaatgcg
      541 ttgaaagtag gtaatggctt tagattgaat cgattgcaaa aatcattcaa gtcgaaatcg
      601 aacgagatat cttttccaga tccaatggcg aggttataat agtttcccag agcattgtaa
      661 acatttttaa ttactccata gcttggccat gaattattaa aattttcgct ggcatcactc
      721 atatcggatt catcataaag caatatggca tacgataatt ttccatctct tccggcgcgc
      781 cctgcttcct gaaaatatgc ttcgggagaa tcgggaatat ccatgtgaac gacaaacctt
      841 acattgggtt tgtctattcc cataccaaat gcatttgttg ctaccattac ttttaattca
      901 tcgttcatcc agcggttttg tacattattc ctttgcttca tggacatccc tgcatgatag
      961 acgccggccg aatgatggtt ccgaaccagg aattttgcta tttcaacggt tctgcgtctg
     1021 ttccgaacgt aaacgatccc cgagcctttt aaattgctgc agattttcaa taaacgtctc
     1081 gccttatcac cttccttttg taccacatat attagattgg gacgataaaa gctacgactc
     1141 aatacatttt tttccttaaa ctgcagcttc tcctgtatgt cgttgatgac catcgaagtg
     1201 gctgttgcag taagggcaag
//
LOCUS       DAORPD010000668         1219 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_23779_length_1219_cov_1.719072, whole genome shotgun sequence.
ACCESSION   DAORPD010000668 DAORPD010000000
VERSION     DAORPD010000668.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23779_length_1219_cov_1.719072"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1159)
                     /locus_tag="PK976_07705"
     CDS             complement(<1..1159)
                     /locus_tag="PK976_07705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRC67810.1"
                     /translation="MLNTLDPYTILIPEDQIQNYRFFMSGEYGGIGITSLQRANGDCT
                     IIDVKEDSPASRAGLKPGDLIVEINNIDLKDKTDEDIDILIKGEVGSPISLSIMKYKS
                     NQIEKLTLLRENIQLPNISYSGMIGDDIGCIRLEQFTDKSSKEFYEAFQKLRQSKNLK
                     GLIIDLRGNGGGLLDQAVTIVNQFVDKGLLIVSTRGRNIDRNRNYYTTNPPLDLSIPL
                     VVLVDKYTASASEILAGALQDLDRAVVIGEKTFGKGLVQNILPLPYNNQLKITIAKYY
                     IPSGRCIQAIDYFKHDQNRTSVQTKPVFYTHNGRMVFEQGGIEPDLPSDSVSNNPLIN
                     SLFQNFIIFDFVNEFAYQHPHIDPLEKFTLDNKIFNDFIQFTTDRYKDWDYE"
BASE COUNT          322 a          223 c          270 g          404 t
ORIGIN      
        1 tttcatagtc ccaatcttta tagcgatcgg tagtaaattg aatgaaatca ttaaagatct
       61 tgttatccag agtaaacttt tccaaaggat cgatgtgcgg atgttgataa gcgaactcat
      121 tcacaaaatc gaaaattata aaattctgaa ataatgaatt gataagagga ttgttagata
      181 ctgaatcaga aggaaggtcg ggttcaattc ctccttgttc gaaaaccata cggccgttgt
      241 gggtatagaa tacaggtttg gtttgcacag atgttcgatt ttggtcgtgt ttgaaataat
      301 cgatggcttg aatacatcgc ccacttggaa tgtaatattt ggcaatggta atcttcaatt
      361 gattattgta tgggagaggc aagatgtttt gaaccaaccc ttttccgaag gttttttctc
      421 ctataactac tgccctgtca aggtcttgca aggcacctgc gaggatttca gaagccgatg
      481 cagtatattt atctacaagt actaccagtg ggatagagag atcgagggga gggttggtcg
      541 tataataatt tcggtttcgg tcgatattac ggccgcgtgt acttacgatc aataatcctt
      601 tatcgacgaa ttgattgaca atggttacgg cctgatcgag caaaccgcct ccattccctc
      661 taagatcgat gattaatccc ttgagatttt tgctttgacg aagtttttgg aaagcctcgt
      721 aaaattcttt ggatgattta tcagtaaatt gttccagacg aatgcagccg atgtcgtcgc
      781 ctatcattcc ggaataggaa atgttgggaa gttgaatgtt ttctctcagc agagtcaatt
      841 tctcgatttg attactttta tatttcatta tggagagaga tattgggctt cctacttctc
      901 cttttataag aatgtcgatg tcttcgtcgg ttttatcttt caggtcgata ttgttgattt
      961 ccacaatcaa atcgcctggt ttcaagccag ctcgtgaagc cgggctgtct tctttcacgt
     1021 caatgatggt acaatcgcca ttggctcttt gcaagcttgt gatgccaatt cctccatatt
     1081 ctccactcat gaagaatctg taattctgta tctggtcctc tggtattaat attgtataag
     1141 ggtccagagt atttagcatg gatttgatcg cagtagaggt cagcttaccg gggtcgatct
     1201 ggtcggcata gttgatctg
//
LOCUS       DAORPD010000669         1214 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_23933_length_1214_cov_2.644521, whole genome shotgun sequence.
ACCESSION   DAORPD010000669 DAORPD010000000
VERSION     DAORPD010000669.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23933_length_1214_cov_2.644521"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1069
                     /locus_tag="PK976_07710"
     CDS             <1..1069
                     /locus_tag="PK976_07710"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRC67811.1"
                     /translation="ANVSVLYAHPIINKKILSPRWEFSTFRDVDELRIYYSGLSRNFP
                     GSQIIKASQYLFHLIAGYLWLQKRTKRFKLAHVNILTRTAIFASVLKNLTGLPYLITE
                     HWSRYLPITNTYRGILRKIVTRYVVRRAGAITVVTENLKNAMQRHGLHHKRFYIIPNV
                     VDTNIFTPTFLNHSSKKLLHVSCFEDKSKNISGLLRATQKLSHLRNDFELVLVGDGQD
                     FQKIKEYSQNLHLEAYVTFTGLIEKEEKLAECYRQSCCLVMFSNYENMPVVINESLAC
                     GKPVIATRVGGIPEIIDSAKGILIDPKNEDSLCEAMNYMLDHASEYDSQALRNYAVEN
                     FSFSKVGECFLKIYQEIKPIQ"
BASE COUNT          387 a          215 c          242 g          370 t
ORIGIN      
        1 tgcaaatgtt tcggttttat atgctcatcc tattataaac aaaaaaattc tttctccgag
       61 atgggaattc tcgacttttc gagatgtgga tgagttacga atctattatt ctggcttatc
      121 acgaaatttt cctggcagtc aaattataaa agcgtcgcaa tatttgtttc atttgatagc
      181 aggttattta tggttgcaaa aacgaacaaa gcgtttcaaa ctggctcatg ttaatattct
      241 tacccgaacg gctattttcg cctctgtgtt aaaaaacctt acaggattgc cctacctgat
      301 taccgaacac tggtcgaggt acttgcctat tacaaataca tataggggaa ttctgcggaa
      361 aatcgtgact cgctatgtag tgagaagagc tggtgcaatt actgttgtaa ccgaaaatct
      421 caaaaacgcc atgcagcgtc atggacttca tcataaaaga ttctacataa ttcccaacgt
      481 tgttgatacg aacatcttca cacctacttt cctaaatcat tcaagtaaaa agttgctgca
      541 tgtttcctgc ttcgaagaca aatcgaagaa tataagtggg ctacttagag caacacaaaa
      601 actctctcat cttcgtaatg attttgagtt agtccttgtg ggtgatgggc aggattttca
      661 aaaaataaaa gaatacagcc agaatcttca tttagaagct tatgtaacct ttactggatt
      721 aatagaaaaa gaggagaaat tagccgaatg ttatcgtcaa agttgttgtt tggtaatgtt
      781 tagcaattat gaaaatatgc cagtagtcat caacgaaagt ttggcctgtg gtaaacctgt
      841 gattgcaacg cgagttggtg gaattcctga aattatagat tctgccaaag gtattttgat
      901 cgatcctaag aatgaagatt cgctctgtga agctatgaat tatatgttgg accatgcatc
      961 ggaatatgat agtcaggctt tacgaaatta tgcggtcgag aactttagct tttcgaaggt
     1021 gggagaatgt tttttaaaaa tttatcagga aataaaaccc atacaatgac attaggagaa
     1081 agaaaagggc tgaaatttgg cattttatgc gatacactgc atcttcagca gtggcaaact
     1141 ttagtcatag aacagctgat tttggaaggg aatgcccttg ctctcatcat tctcaacaaa
     1201 gaagactcgc cacc
//
LOCUS       DAORPD010000670         1213 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_23977_length_1213_cov_1.876511, whole genome shotgun sequence.
ACCESSION   DAORPD010000670 DAORPD010000000
VERSION     DAORPD010000670.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23977_length_1213_cov_1.876511"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="PK976_07715"
     CDS             complement(<1..388)
                     /locus_tag="PK976_07715"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HRC67812.1"
                     /translation="MKTKILVTGSNGQLGNELRLLSAHLNHLSFVFTDVTELDITQPE
                     AVNEIMESLQPQYLINCAAYTAVDKAESEKESAWKINAIAPEILANSARKTGTCLIHI
                     SSDYVFDGQNYRPYLPSHPTHPLSWYG"
     gene            complement(432..>1213)
                     /locus_tag="PK976_07720"
     CDS             complement(432..>1213)
                     /locus_tag="PK976_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRC67813.1"
                     /translation="LIRHDVTMPFFIEADEIYNLACPASPIHYQYNPIKTIKTSVMGA
                     INMLGLAKRIRVKILQASTSEVYGDPDVHPQTEDYWGKVNPIGPRACYDEGKRCAEAL
                     FMNYYRQNNVRIKIIRIFNTYGPKMHPNDGRVVSNFIVQALKNQNITIYGDGSQTRSF
                     QYVDDLIEGAIRMMDTPDDVTGPVNIGNPDEFTILELAEKVIRLTGSRSKIIFQPLPP
                     DDPKQRQPDITLAKNLLNWEPKVALEDGLQMTIEYFRKLIR"
BASE COUNT          317 a          231 c          274 g          391 t
ORIGIN      
        1 tcccatacca cgacaaagga tgagtggggt gagaagggag gtaaggtcta taattttgcc
       61 catcaaaaac atagtcactg gaaatgtgaa taaggcaagt accggtttta cgtgctgaat
      121 tggcgagtat ttctggagct atggcattga ttttccaagc ggattctttt tcactttcgg
      181 ctttatcgac tgctgtataa gctgcacaat tgatgagata ttgaggctgg aggctttcca
      241 ttatttcatt tactgcctcg ggttgggtaa tatccaattc ggtaacatcg gtaaacacaa
      301 aagataaatg attcagatgt gcactcaaaa gcctcagctc atttccaagc tgaccattgc
      361 tacctgttac gagtattttg gtcttcattt caataatttt gtttgctttt cttctattat
      421 tttatccagt ttcagcgtat aagcttgcgg aaatattcta tagtcatttg gaggccatct
      481 tcaagtgcga ctttgggttc ccaattcaat agatttttag ccagggtaat atcgggttgg
      541 cgttgtttgg gatcatcggg cggaagaggt tgaaatataa tctttgagcg gctaccagtg
      601 agccgaatga ctttttcagc tagctcgaga atggtaaatt catctggatt tccaatgtta
      661 acaggaccgg ttacatcgtc aggcgtatcc atcattctaa tggctccttc gatcagatcg
      721 tctacgtact gaaaagaccg tgtttgagaa ccatcaccat agatggtgat attttgattt
      781 tttaatgctt gaacgatgaa atttgaaacc accctgccat cgttggggtg catttttggt
      841 ccataagtat taaagatgcg gataattttt atgcgaacgt tgttctgacg gtagtaattc
      901 ataaataaag cttcggcgca tcttttccct tcgtcgtaac atgctcttgg ccctatggga
      961 tttacctttc cccaataatc ttctgtttga ggatgaacat cgggatcgcc ataaacttcg
     1021 ctggtggaag cctgaagaat tttgacacgg attcgcttgg ccaagcctag catgttgata
     1081 gcccccatta cggaagtctt tattgtttta atgggattgt actgataatg gataggagaa
     1141 gcagggcatg ccagattata aatttcatct gcttcgataa aaaagggcat ggttacgtcg
     1201 tgcctgatca gct
//
LOCUS       DAORPD010000671         1212 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_24002_length_1212_cov_3.022472, whole genome shotgun sequence.
ACCESSION   DAORPD010000671 DAORPD010000000
VERSION     DAORPD010000671.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24002_length_1212_cov_3.022472"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..858
                     /gene="fabG"
                     /locus_tag="PK976_07725"
     CDS             112..858
                     /gene="fabG"
                     /locus_tag="PK976_07725"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007652512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HRC67814.1"
                     /translation="MKLLEGKTALITGAARGIGRSIALKFASEGAHIAFSDLRLDEAT
                     QILEEEIRGMGVEAMAYASDASSFSDSEILVTQVIEDFGTIEILVNNAGITRDNLLMR
                     MSEADWDLVMKVNLKSVFNLTKAVQKIMLKNRFGSIINMSSVVGVKGNAGQANYSASK
                     AGIIGFTKSMAQELGSRNIRCNAVAPGFIDTEMTAKLSEEVKREWAEKIPLRRAGLPE
                     DVANSCLFLASDLSSYITGQVIHVCGGMVM"
     gene            877..>1212
                     /locus_tag="PK976_07730"
     CDS             877..>1212
                     /locus_tag="PK976_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67815.1"
                     /translation="MSFIFENSGWWLVLAPVVGFVYAFVLYRNTHDIFDRKIQILLGI
                     LRFVVVTILVIVLMGVLIKLTSHRFEKPLVVLALDNSQSMAQLPDSTEIRKNLPGLLK
                     NLESKLAPEF"
BASE COUNT          335 a          209 c          287 g          381 t
ORIGIN      
        1 ttagtcgagg gttttctttt ggctttgatt tttcgagctg gatgacagaa aagactcaac
       61 aaccagtttt attttaaatt tgcagtggaa acggaataaa atcattttcg catgaaatta
      121 ttggaaggta aaactgcttt gattactgga gcggccagag gtataggtcg ttctattgcc
      181 ttgaagtttg cttcggaagg ggctcacatt gccttttccg atcttcgttt ggacgaagca
      241 acgcaaattt tggaagaaga aatccgagga atgggtgtcg aagctatggc ttatgcttcg
      301 gatgcttctt ctttcagtga cagtgagata ttggttacgc aggttataga ggattttggt
      361 acgattgaaa ttttagtgaa caatgcaggc attacgcgtg ataatttgtt aatgcgcatg
      421 agtgaagctg actgggactt ggtaatgaaa gtaaatttga aatcggtttt caatctaacc
      481 aaagctgttc aaaaaattat gttaaaaaac agattcggaa gcatcatcaa catgtcgtca
      541 gttgttggag tgaaagggaa tgctgggcaa gccaattatt cagcttcaaa ggctggtatc
      601 attggtttta cgaagtcaat ggcacaagaa ctgggttcgc gtaatattag atgtaacgca
      661 gtggctcctg gttttattga tactgaaatg acggcgaaac tttcggagga agtaaaaagg
      721 gaatgggccg aaaaaattcc tttgaggcgt gctggtctgc ccgaagatgt tgccaattcc
      781 tgccttttcc ttgcttctga tttgtcatct tatataaccg gccaagtgat acatgtttgt
      841 ggcggaatgg ttatgtaaac aacatcactc attaagatgt cgtttatttt cgaaaattcc
      901 ggctggtggt tggtgttagc ccctgtggtg ggcttcgttt atgcttttgt gttatatcgc
      961 aacacacacg atatcttcga ccgaaaaatc cagattctgc tcggaatatt acgttttgta
     1021 gtggttacaa ttctggttat cgttttgatg ggagtactca taaaacttac ctcacatcgt
     1081 ttcgaaaagc ctttggtggt tttggcgctc gacaatagcc aatcaatggc tcaattacct
     1141 gattcgaccg aaatcaggaa gaatttacct ggacttttga aaaatctcga atcaaaactc
     1201 gcacccgaat tt
//
LOCUS       DAORPD010000672         1212 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_24004_length_1212_cov_2.535004, whole genome shotgun sequence.
ACCESSION   DAORPD010000672 DAORPD010000000
VERSION     DAORPD010000672.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24004_length_1212_cov_2.535004"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1178)
                     /locus_tag="PK976_07735"
     CDS             complement(<1..1178)
                     /locus_tag="PK976_07735"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HRC67816.1"
                     /translation="MNIWLHFIAIGGSAMHNLALALAEKGYTITGSDDVIFEPSRSRL
                     ARKGLLPEREGWFPEKLDPNIDAVILGMHAKADNPELLRAKELGLKIYSYPEYLYEQS
                     KDKMRIVIGGSHGKTTITAMVLHAFRQAGIDTDYMVGARLEGFDVMVKLSHNAHFMVI
                     EGDEYLTSPIDPRPKFHLYHPHIALLSGIAWDHFNVFPSFEIYRKQFEIFVSLIEPAG
                     ALVYCKEDESVRRIASNAPNNIQKISYSFPEFKIQDHTTLIKAIDEWFPLQIFGKHNL
                     LNLSGAMKVCQLIGIDTLTFMKAMTSFRGASNRLELIAQNELTAVYRDFAHSPSKVKA
                     TIEALVGQYPERNLVACLELHTFSSLNKDFLPQYKGSMEMAHKRIIYFNPHSLQFKNL
                     P"
BASE COUNT          348 a          231 c          261 g          372 t
ORIGIN      
        1 gggaggtttt tgaactgcaa ggaatgagga ttaaaataga ttattctttt atgagccatt
       61 tccatgcttc ccttatactg aggaagaaaa tctttgttga ggctgctaaa ggtatgcaat
      121 tcaagacaag ccacgagatt tcgttcgggg tactggccaa caagggcttc tattgtggct
      181 ttaactttgg aaggtgaatg tgcaaagtcg cgataaactg ctgtaagttc gttttgggct
      241 atcagttcca gacggtttga tgcaccacga aaacttgtca tggctttcat gaaagtgaga
      301 gtatcgattc ctattaattg gcaaaccttc atagccccac tcaaattcaa aagattatgt
      361 ttcccgaata tttgcaaggg gaaccattcg tcgatggctt ttatcaaggt tgtatggtct
      421 tgaatcttaa attctgggaa agaataggaa attttttgga tgttattggg agcattcgag
      481 gcaattcgtc taaccgattc atcttctttg caatacacaa gagcgccagc aggttcgatt
      541 aaagaaacga agatttcgaa ttgttttcta taaatttcga atgaagggaa cacattgaaa
      601 tgatcccatg ctattccact cagcaatgca atatgaggat gatagagatg aaatttgggg
      661 cgtgggtcaa taggtgaagt aagatattca tctccttcaa tcaccataaa atgagcatta
      721 tggcttagtt ttaccattac atcgaaacct tcgaggcgag ctccaaccat ataatcggta
      781 tctatgcccg cttgtcggaa agcatgcaag accatggcag taatggtggt tttgccatgg
      841 cttcccccta tcactattct cattttatct ttcgattgct cataaagata ttcgggatag
      901 gaatagattt tcaatccaag ttcttttgcc ctgagcaatt ctggattgtc ggccttggca
      961 tgcattccaa ggataactgc atcaatattg ggatcgagtt tctctggaaa ccatccttct
     1021 ctttcaggga gtaaaccttt tcgggcaaga cgtgaacgtg aaggttcgaa aattacatca
     1081 tcgctgcctg ttattgtgta tcctttttca gcaagagcca aagccagatt gtgcatggca
     1141 ctgcctccaa ttgcaatgaa atgtagccaa atgttcattc aagatttctt tcggtaaatt
     1201 aaaactacga aa
//
LOCUS       DAORPD010000673         1210 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_24067_length_1210_cov_2.128139, whole genome shotgun sequence.
ACCESSION   DAORPD010000673 DAORPD010000000
VERSION     DAORPD010000673.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24067_length_1210_cov_2.128139"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="PK976_07740"
     CDS             <1..572
                     /locus_tag="PK976_07740"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRC67817.1"
                     /translation="AKIESGAPDIAIQAAAEDFQADLIVLGAVGLGRKDHFSGGTAEY
                     LMKHSQKPVLAIPVNCGYKGLNKAIYAVDPAHFHKAELNRIQEFFKPFHAEIHYLVLC
                     YNPKNRPNDSTIAEWQNLVSNRASFQIVDCDHPQEGLHLSIRNIQPDIIAFHFFRESL
                     FSRWFKPALSRKDLYKANLPLLSFPEIEE"
     gene            569..1075
                     /locus_tag="PK976_07745"
     CDS             569..1075
                     /locus_tag="PK976_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018709531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HRC67818.1"
                     /translation="MIQIGLLSDTHGVLLPFVIKALDHVDEIWHAGDIGHVEVYEQLR
                     KMKPLRAVHGNIDSWTLRCQCPEYLSFMIEDVKVLMIHKAGSPGKYSPACRKLIISEK
                     PGLLIGGHSHILKVMQDNSNNLLFLNPGAAGLEGIHKVMTLLRFSLDKGKVVNMEIIE
                     KERSPSRI"
BASE COUNT          358 a          243 c          247 g          362 t
ORIGIN      
        1 atgcgaagat agagtcgggt gcacccgata tagctattca agctgctgcc gaagattttc
       61 aggccgacct cattgtttta ggagcagttg gtttgggacg taaagaccat ttttcgggag
      121 gtacggccga atatcttatg aaacattctc aaaaacccgt tttggccatt ccagttaatt
      181 gtggttacaa aggattgaac aaagcaatct atgctgtcga cccggcacat tttcataaag
      241 ctgaactcaa ccgaattcag gaattcttca aaccatttca tgccgaaatc cattatcttg
      301 tattgtgtta taatcccaag aatcgtccta atgactctac catagctgag tggcaaaatt
      361 tagtgagtaa tcgggcttcg ttccagatcg tcgattgcga tcatcctcag gaaggtcttc
      421 atctttctat tcgaaacata caacccgaca tcattgcatt tcattttttc agagaaagcc
      481 tcttttcacg ctggtttaaa ccagccctct cgcgtaaaga tctatataag gcaaatcttc
      541 cgcttttgtc attccccgaa attgaagaat gatacaaata ggtttacttt ccgatacgca
      601 tggtgtttta ttgccatttg taatcaaagc tctcgatcat gtagatgaaa tttggcatgc
      661 cggcgatatt ggtcacgtag aggtttatga acagcttcga aaaatgaagc ctttaagggc
      721 agttcatgga aatattgata gttggactct tcgttgtcaa tgccccgaat atctttcatt
      781 tatgattgag gatgtaaaag tattaatgat acacaaagct ggaagtccag gaaagtattc
      841 tcctgcatgc cgtaaactta ttatttcgga gaaacctggt ttgctgattg gtggtcactc
      901 tcacatcttg aaagtaatgc aggataattc gaacaattta ctattcctta atcctggtgc
      961 tgcaggactc gaggggatcc ataaagtaat gactctgctc agattttcct tggataaagg
     1021 gaaggttgtg aacatggaaa ttatcgaaaa agaacgatct ccctcgagaa tataatctta
     1081 tgagctaaga taaaaaataa ggaatcaatc ggtgttttat gggttgaagt gaaaccgaca
     1141 aagtcaggaa agcatgacct gaacactttc ctcacacttg catagcccag atttcaccta
     1201 cgttattgcc
//
LOCUS       DAORPD010000674         1206 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_24199_length_1206_cov_2.349262, whole genome shotgun sequence.
ACCESSION   DAORPD010000674 DAORPD010000000
VERSION     DAORPD010000674.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24199_length_1206_cov_2.349262"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1047
                     /locus_tag="PK976_07750"
     CDS             <1..1047
                     /locus_tag="PK976_07750"
                     /inference="COORDINATES: protein motif:HMM:NF027451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4837 family protein"
                     /protein_id="HRC67819.1"
                     /translation="AMKKLLTVIIVFLSLITATSCSKSSSKKSSTGGTLEVLVATDSH
                     EQWNGKIGDTIRSLFARYIPCLPMPEESFTPLPILSKTLYENDMYKAHHNIFIVSIDP
                     KLTKPSINLERNYWAEPQLIIHFSCPSDTSFFCLFNQYYPSLYELYREVEKDRIQKLF
                     TSNPAKEIITKLQSKYGIDMLIPGGFTVAREEENFIWIRQTIHRRKQDTETGFLVYYR
                     PYTDTAQFNGKNIIRTRDSITRKYIPGAVEGSYMATSVDVIPPQFKRTSKFSTGFAVE
                     TRGLWKVVNDFMGGPFISYTFADTKRQRIVTVEGYLYHPNSEQRKYMLQIESLLNTAK
                     VYDEQEQQNVPTKK"
BASE COUNT          393 a          246 c          202 g          365 t
ORIGIN      
        1 gccatgaaaa aattattaac ggttattata gtttttctat cattgataac tgctacatcc
       61 tgttccaaaa gcagttcaaa aaaatcttcg accggtggta cactcgaagt tttggttgcc
      121 accgatagtc atgagcaatg gaatggtaaa atcggggata ccatacgttc tctgttcgct
      181 cgatatattc cctgtcttcc tatgcctgag gaatcgttta ctcctttacc aatactttcc
      241 aagactcttt acgaaaatga tatgtataag gctcatcaca atatctttat cgtaagtata
      301 gacccaaaat taacaaaacc atccataaat ctggaaagga attattgggc tgaaccccaa
      361 ttgatcattc acttttcttg tccatccgat acttcctttt tttgcttgtt taatcaatac
      421 tacccttctt tatatgaact ttacagggag gtagaaaaag atcgtattca gaaacttttt
      481 acctcaaatc cggccaaaga gattattact aagcttcaat ccaaatatgg aatagatatg
      541 cttattcccg gcggattcac agttgccaga gaagaagaaa attttatttg gataagacaa
      601 actattcacc gtcgcaagca agacacagaa acgggatttc tggtatatta caggccatat
      661 accgatacgg ctcagtttaa tggtaaaaac attattcgta cacgagattc tattacccga
      721 aaatatattc ctggagcagt cgaaggttca tatatggcca cttcggtaga cgttattcct
      781 ccacaattta agcgaacctc taaattttcg acgggttttg ccgtcgaaac acgtggactc
      841 tggaaagtag taaacgattt tatgggtgga ccctttatca gctatacttt tgccgataca
      901 aaacgtcaac gaatcgttac tgtagaaggt tatctatatc atcccaattc ggaacagaga
      961 aaatacatgc ttcagatcga aagccttctc aatactgcta aggtttatga tgaacaggaa
     1021 caacaaaatg tcccaactaa aaaataatct ataaactata atgaactata catctttatc
     1081 tttgtcgaaa aagaaattac tggtactgtt tttattgctg aattatgtgc tcatttcgca
     1141 agctcaggaa aaacacttta ctgctgaaga tgcttcatac aataatccgg cactataccc
     1201 caaagg
//
LOCUS       DAORPD010000675         1204 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_24274_length_1204_cov_2.273281, whole genome shotgun sequence.
ACCESSION   DAORPD010000675 DAORPD010000000
VERSION     DAORPD010000675.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24274_length_1204_cov_2.273281"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="PK976_07755"
     CDS             <1..341
                     /locus_tag="PK976_07755"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRC67820.1"
                     /translation="WSQEQLGEMAEISRNYVSQIERGEADSISIKVINKLAIALGVSL
                     TELGESSSPSTVMIPPSLREFALQDNLPYEVIDKLVNIPRRGKEPKTVAEWRVLYEAI
                     SSYIDGNRKE"
     gene            338..505
                     /locus_tag="PK976_07760"
     CDS             338..505
                     /locus_tag="PK976_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67821.1"
                     /translation="MTFIDPGEIFVKTFGKIETENDVFAYVDLAWYQTQNLPFNLEDE
                     SQVLSLIEYLG"
     gene            514..993
                     /gene="brxF"
                     /locus_tag="PK976_07765"
     CDS             514..993
                     /gene="brxF"
                     /locus_tag="PK976_07765"
                     /inference="COORDINATES: protein motif:HMM:NF033453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-3 system P-loop-containing protein BrxF"
                     /protein_id="HRC67822.1"
                     /translation="MAIDTLAKLLSQPYDCLILVHPYIQILEKLSTEIQGLGLLHLNI
                     SKELSTFLMTISASERSRFSQKWLLDTLITFQNGPVLCTCPDLLFDPSLKIDPLSLIR
                     QAARIKQLIVLWPGEYSANTLSYAVPEHHHFRTWKVTDSFLRQPVVLIRQISTSQGA"
     gene            998..>1204
                     /locus_tag="PK976_07770"
     CDS             998..>1204
                     /locus_tag="PK976_07770"
                     /inference="COORDINATES: protein motif:HMM:NF039983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6079 family protein"
                     /protein_id="HRC67823.1"
                     /translation="MLYRDLVQFTPIETIIQLRDAEKESAAKNLVQTYVISDAMANKL
                     VDMAFPQLQIDRPQDNKGILIVGNY"
BASE COUNT          360 a          258 c          233 g          353 t
ORIGIN      
        1 ggtggagtca agaacagctt ggtgagatgg cagaaatctc acgaaattat gtatcccaaa
       61 ttgagcgagg cgaagctgat agtatttcca tcaaagtgat taacaagtta gcaatcgctt
      121 taggcgtctc acttactgaa ctcggcgaat cttcatctcc atccactgtt atgattccac
      181 cttctttacg ggagtttgct cttcaagata atctgcctta tgaagtaatt gataaattgg
      241 tgaatattcc aagacgaggg aaagaaccaa aaaccgttgc cgaatggcga gtattatatg
      301 aggcaataag ctcatatatt gatgggaata gaaaagaatg acttttatcg atccgggtga
      361 aatatttgta aagacattcg gtaagattga gacagaaaat gatgtctttg cctatgtcga
      421 tttggcctgg tatcaaaccc aaaatcttcc tttcaacctt gaagatgaat cacaagtatt
      481 atcattgata gaatatctgg gttaggggaa accatggcga ttgacacttt agctaagtta
      541 ctttcacaac cctatgattg tcttattctc gttcatcctt atatccaaat tcttgaaaaa
      601 ctatcaactg aaattcaagg actggggctt ttacacttaa atattagtaa agagttgagt
      661 acttttctca tgaccatttc agcgagtgag cgcagccgtt tttcgcaaaa atggttgctt
      721 gacaccctta ttacattcca aaacggtcca gttttatgca cgtgccctga tttattgttt
      781 gatccctctc tcaaaattga tccactgtca ctaatccgtc aggctgcccg aatcaagcaa
      841 ttgatagtcc tgtggcctgg agaatattct gcaaacactc tttcctatgc tgtcccggaa
      901 catcatcatt tccgaacctg gaaagtaacc gattcatttc ttcggcagcc cgtagtgttg
      961 attcgccaaa tttccacttc acaaggagca taaagccatg ctatatcgag acctcgttca
     1021 atttacaccc atcgaaacaa ttattcaact gcgagatgct gaaaaagaat ctgccgcaaa
     1081 gaacctggtg cagacctatg tcatttcaga tgccatggcg aataagctgg tcgatatggc
     1141 cttcccccag cttcaaattg accgacctca agataacaaa ggcattttaa tcgttggcaa
     1201 ttat
//
LOCUS       DAORPD010000676         1202 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_24348_length_1202_cov_2.578901, whole genome shotgun sequence.
ACCESSION   DAORPD010000676 DAORPD010000000
VERSION     DAORPD010000676.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24348_length_1202_cov_2.578901"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1061
                     /locus_tag="PK976_07775"
     CDS             <1..1061
                     /locus_tag="PK976_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67824.1"
                     /translation="GNIMKNRLITVIGVLVVVLGACETDIDVNADWKDITTVYSILDP
                     AEKTQYVRINKAFLGEGNALEYAQIADSINYDTSYIEARIDEYLKGELVQSMKLKPLW
                     IPRKNDPNSPFYNPNFPYVLVYMAQPKNFYKMSGNDTIWLNPDSDYHLEIFNRHSGKT
                     VTASTIIVKDFNISRPLYNPSKPFLKILNTDGQNTIKWRAAVNGRRYQVIYRFYYKES
                     PNPGDTVIKTTDYHLGVIKSQYTTGSEDMDVSFMGTSFYKYLGSSIPANPNVKRIAYQ
                     IELIIAVIPEEFNTYLEVNEPSSSIVQDRPEYTNIENGYGLFSSRYIKSRLYFVDSEM
                     AAGIKTYFQTIDGSFIVP"
BASE COUNT          401 a          228 c          225 g          348 t
ORIGIN      
        1 gaggaaatat tatgaaaaat agattgatta cggtgatagg tgttttagta gtcgtcctgg
       61 gtgcatgtga aaccgacata gatgtaaatg ccgattggaa agatataact actgtttata
      121 gtattctcga cccggctgaa aaaacgcagt atgttcgcat caacaaggcc tttctgggcg
      181 aaggcaatgc attggaatat gctcagatag cggattctat caattatgat acttcttaca
      241 tagaggcacg tatagatgaa tatcttaaag gtgagctcgt tcagtcaatg aaactgaagc
      301 ccttatggat tccacgaaaa aatgacccca atagcccctt ttacaatccc aatttccctt
      361 atgttctggt ttatatggct caaccaaaaa atttctataa gatgtctgga aacgatacca
      421 tctggctaaa tcccgatagc gactatcatc tggaaatctt caatcgccat tcgggaaaaa
      481 ctgttactgc ttccaccatt atagtgaagg atttcaacat ttcgcgtcct ttgtataatc
      541 ccagcaaacc ctttttgaaa attctgaata ccgatggcca gaacaccatc aaatggagag
      601 ctgcagtgaa tgggagaaga tatcaggtga tttacaggtt ttattacaaa gaaagcccaa
      661 atccaggcga tacggtaata aaaactaccg actatcatct cggagttatt aaaagtcaat
      721 atactacagg aagtgaagat atggacgtta gtttcatggg tacatctttc tataaatatt
      781 taggatcctc tattcctgcc aatcccaatg tgaaaaggat agcatatcag atagagctga
      841 ttattgcagt aattcccgaa gaattcaaca cctatctgga agtgaacgaa cccagttcga
      901 gtatcgttca agacaggccc gaatatacca atattgaaaa tggatatggt ttattttcaa
      961 gccgttatat aaaatcacgt ttatattttg tagattccga aatggcagca gggataaaga
     1021 cgtacttcca aactattgat ggcagtttta ttgtcccctg atttttccat ttcgaataaa
     1081 aacctcttaa acaaactgcc agaatgacag agcgaacagt ttcgatatca ttcttatttc
     1141 atattaacaa tcaatcgaat aataattttt ttaccacaaa atcggtgaaa gaaggcagta
     1201 ct
//
LOCUS       DAORPD010000677         1202 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_24350_length_1202_cov_2.284220, whole genome shotgun sequence.
ACCESSION   DAORPD010000677 DAORPD010000000
VERSION     DAORPD010000677.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24350_length_1202_cov_2.284220"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="PK976_07780"
     CDS             complement(<1..355)
                     /locus_tag="PK976_07780"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRC67825.1"
                     /translation="MKTIADQIKEISENIHPSLIQIRRQLHQYPELSGEEFGTSAFIS
                     HTLNEWGIDQRTGIAGTGIMAILRGKIPGNACVALRADMDALPIDEQNEVEYCSKNPG
                     IMHACGHDVHVSSLLG"
     gene            complement(367..>1202)
                     /locus_tag="PK976_07785"
     CDS             complement(367..>1202)
                     /locus_tag="PK976_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRC67826.1"
                     /translation="FYNIQKGAASVDRINQILKAEEVIVEKPNAFPKKSFEQRIEYRN
                     VSFRYEEELVLRNINLIIEKGQTVAIVGQSGSGKSTLVDLLPRFYDCTEGCILLDGID
                     IRDYRIDDLRGLMGIVSQETILFNDTVFNNIALGMKKVSQHEVEKAAKIANAHEFIMQ
                     MPEGYQTLIGDRGTKLSGGQRQRLSIARAVLKNPPILILDEATSALDTESERLVQQAL
                     DKLMENRTSIVIAHRLSTIRFADMIVVLHQGEIVEKGKHETLYTQGEFYRKLCDMQSF
                     L"
BASE COUNT          305 a          272 c          246 g          379 t
ORIGIN      
        1 tgccaagtag ggaacttaca tgtacatcgt gcccacaggc atgcattatg cccggatttt
       61 tcgaacagta ttccacctca ttttgttcgt ctatgggcag ggcatccatg tcggcacgca
      121 aggctacgca agcatttcca ggaattttcc ctcttaaaat ggccatgatt cctgtgcctg
      181 ctattcctgt gcgttgatct atgccccatt cattcagggt gtggctgatg aaggcagaag
      241 ttccaaattc ctcgcccgat agttccggat attgatgaag ttgccttctt atctgaatga
      301 gagaaggatg aatattttcg gaaatctcct ttatttggtc ggcaatagtc ttcatgtttc
      361 gatttattat agaaacgact gcatatcaca taattttcga tagaattcgc cttgggtata
      421 tagcgtttca tgctttcctt tttccactat ttctccctga tggagcacta cgatcatatc
      481 ggcaaaacgt atggttgaaa gacggtgggc aattacaatc gatgtacggt tttccattag
      541 tttgtcgaga gcctgctgaa ccagtcgttc actttcagta tcgagagcgg atgtagcttc
      601 atcgaggatg aggatgggag gattctttaa aacggctctg gctatactca ggcgctgccg
      661 ttgtccaccc gaaagttttg taccccgatc accaattaga gtttgatatc cctcaggcat
      721 ctgcatgatg aattcatgag cgttagctat tttcgcagct ttttctacct catgctggct
      781 tacctttttc atcccaaggg ctatattatt gaaaaccgta tcattaaata atatggtttc
      841 ctggctgaca attcccatca aaccccttaa atcatctatc cgataatcgc gaatgtcgat
      901 accatccaga agtatgcagc cttcagtaca atcgtaaaaa cgaggtaaaa gatcgaccaa
      961 ggtggatttc ccactgcccg attgcccaac gatggcaacg gtttgtcctt tttcgataat
     1021 caagttgata ttacgcaaaa ccaattcctc ttcgtatctg aaggaaacgt tccgatattc
     1081 gattctctgt tcaaaggatt tcttgggaaa tgcattgggt ttttcgacaa tcacttcttc
     1141 tgccttcaaa atctggttga ttctatcaac cgaagcagct cctttttgga tattgtaaaa
     1201 tg
//
LOCUS       DAORPD010000678         1200 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_24406_length_1200_cov_2.377293, whole genome shotgun sequence.
ACCESSION   DAORPD010000678 DAORPD010000000
VERSION     DAORPD010000678.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24406_length_1200_cov_2.377293"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            96..398
                     /locus_tag="PK976_07790"
     CDS             96..398
                     /locus_tag="PK976_07790"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRC67827.1"
                     /translation="MSKQKKLDIARLELAASRLRALAHPMRIAIIDMLTSTPKMNVTE
                     IYEKLGIEQAAASHHLNILKNKGILVSKRDGKKIYYSLKNDALTQIVDCLDRCDSH"
     gene            783..>1200
                     /locus_tag="PK976_07795"
     CDS             783..>1200
                     /locus_tag="PK976_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRC67828.1"
                     /translation="MSEFVHLHVHSHYSLLDGAAKISELVKKAKDLGMPAIALTDHGN
                     LYGAFEMHEAASNEGIKPILGAEFYIAKKSRKDHDNRGYHLILLAKNLTGYHNICILS
                     TLAFKEGIYYVPRIDKELLQEYHEGIIASSACLGGEI"
BASE COUNT          393 a          196 c          248 g          363 t
ORIGIN      
        1 ggcattaccc ggaagaataa atcgcttatt tatatatcta cttgtttgaa atatgggaat
       61 gattattttt gcaaaaactt aaagccttaa aaaagatgtc gaaacagaaa aaattagata
      121 ttgctcggct ggaattggcg gcaagcagat tgcgtgcatt ggctcatcct atgcgtatcg
      181 ccatcatcga tatgttgacg agtactccta aaatgaacgt taccgagatt tatgaaaaat
      241 tagggataga acaagccgct gcttctcatc atttgaacat cctgaaaaac aaaggaatat
      301 tggtttcgaa aagagatggg aaaaaaattt actactcttt gaagaatgat gctctaaccc
      361 aaattgtcga ctgcctagac cgttgcgaca gtcattaagt caactccgaa aagatcgtac
      421 ttcctttaga gggttgtagt ctttttgtgt ttcgaaaatg gaaataaaaa aagaatctga
      481 aaaaataggc ttaagggaaa gaattagggg tatattttgt tgaaatataa ttttttcaat
      541 aacgaatagt gagcttccct gtaaaggaag gagattatcc cccatcttaa ttatactcgc
      601 cgtattttta cgtctgagaa gttcttgcct tatcgtacga aggggcattg ttccctcaaa
      661 ttctaaagaa aagttcgagg atttatggct attttttgat attctgtcat tcataatgcc
      721 ggggattggc taattttgga aaccacaatt caattcagaa taaataaaat attgtcttta
      781 taatgagtga gtttgtccac ttgcatgtgc attcccatta ttcgctatta gatggggctg
      841 caaagatttc tgagcttgta aagaaggcta aagatttagg catgcctgcc attgcattga
      901 ccgatcatgg gaatttatat ggtgccttcg agatgcatga agcagcctca aatgaaggaa
      961 taaaacctat tcttggagct gaattctata tcgctaaaaa aagccgtaaa gaccatgata
     1021 atagaggtta ccacttgatt ttacttgcga aaaatttaac aggataccat aatatttgca
     1081 ttttgagtac actggcattc aaagagggca tatattacgt tccaagaatc gacaaggaat
     1141 tgctccagga atatcatgag gggatcatag cttcttcggc atgtctggga ggagagattg
//
LOCUS       DAORPD010000679         1200 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_24414_length_1200_cov_1.863755, whole genome shotgun sequence.
ACCESSION   DAORPD010000679 DAORPD010000000
VERSION     DAORPD010000679.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24414_length_1200_cov_1.863755"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1197)
                     /locus_tag="PK976_07800"
     CDS             complement(<1..1197)
                     /locus_tag="PK976_07800"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HRC67829.1"
                     /translation="MLLYMFNKPLDHAYHFIEKKFSKKPLVVEMNKRVLKAGYDYADT
                     VEILPSRIHVPPAKMPKGKYRNITGNIATAWGLMAASEKSARPLFLGSYPITPASEIL
                     IELAKHRNLGVKTFQAEDEIAGINSAIGASYAGSIGVTSTSGPGLSLKVEALGLAVMT
                     ELPLVVVDVQRAGPSTGMPTKPEQSDLNLALFGRNGEAPLIILSASSPADCFYAAFEA
                     AKLSLEHMTPAILLSDGYIGLGSELFRIPKMADLPEIKPPIAQPNTPYKPYARDPETL
                     VRKWAIPGTEGLRHRIGGLEKTNIDGLVSTDPINHETMVKLRAEKVKRIENFIPLQEV
                     YGESEGRLLVVSWGGTYGAVRNAVDHCQKDGYKIAHAHFRYINPLPRNTAEIFKRYEK
                     VLVCELN"
BASE COUNT          301 a          251 c          309 g          339 t
ORIGIN      
        1 attgagttcg cataccagaa ctttttcgta acgtttgaaa atttctgctg tgttgcgagg
       61 aagaggatta atgtaacgga aatgtgcatg agctattttg taaccatctt tttggcagtg
      121 atcgacggca tttcgcacgg caccataagt tccgccccag ctaactacta aaaggcgtcc
      181 ttcggactct ccgtaaactt cctgcaaggg tataaagttt tcgatacgtt ttactttttc
      241 ggcacgtaat tttaccatgg tttcgtgatt gataggatcg gtcgagacca ggccatctat
      301 gttggttttt tcgaggcctc ctatgcggtg acggagtcct tccgtccccg gtatagccca
      361 tttacgaacg agggtttcgg ggtcgcgggc ataaggttta tacggtgtat tgggttgggc
      421 tatgggaggt tttatttcgg gaaggtcggc cattttgggg attcggaaaa gctccgaacc
      481 aaggccaata tagccatcac ttaataagat agctggagtc atgtgttcaa gggaaagttt
      541 ggcagcttcg aatgctgcat agaagcagtc ggccggagag gaagcagaga gtatgataag
      601 cggggcttcg ccattgcgtc cgaaaagtgc aaggttcaag tcggattgtt cgggtttggt
      661 aggcattcct gtggaaggtc cggcacgctg tacatccacc acaacaagtg gaagctctgt
      721 catgacggcc aggccaagtg cttcgacctt gagcgacaag ccggggcccg aagttgaagt
      781 tactcctatt gacccggcat aactggctcc aattgctgag ttaatcccgg ctatttcatc
      841 ttcagcctga aaggttttta ctcctaaatt gcgatgtttt gcaagctcga tgagtatttc
      901 agaagcaggg gtaataggat atgaacctaa aaaaagtggc cgagccgatt tttcggaagc
      961 agccatcaat ccccaagcag tggctatatt tcccgtaata ttgcgatatt ttcctttggg
     1021 cattttggcc ggaggaacgt ggatacgaga gggtaaaatc tctacggtat cggcataatc
     1081 ataacctgct ttcaaaacac gtttgttcat ttcgaccact aaaggtttct tgctgaattt
     1141 tttctcgatg aaatgataag catgatccag gggtttattg aacatatata gcaacatacc
//
LOCUS       DAORPD010000680         1193 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_24659_length_1193_cov_2.571178, whole genome shotgun sequence.
ACCESSION   DAORPD010000680 DAORPD010000000
VERSION     DAORPD010000680.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24659_length_1193_cov_2.571178"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..>1193)
                     /locus_tag="PK976_07805"
     CDS             complement(105..>1193)
                     /locus_tag="PK976_07805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HRC67830.1"
                     /translation="DVLQVLDQYAPFVYQEEYDNSGFQLGDKDEEVHGILLCLDLTTE
                     IIDEAIQNRFNLILTHHPFFFKGLRYLDFSKSEGRIIRKLTENRISLISFHTNFDNVA
                     KGTNEALANKLGLKDTMILCPKKGALRKLVTFCPVGFSEKVRSALFSVGGGYIGNYDS
                     CSFNAEGYGTFRAQEGSHPYVGEINQIHKELETRIEVIFPVHLTDTIIRLLLEVHPYE
                     EVAYDIIPLDNVYTSVGEGIIGNLPQNFELMDFLAFLKSSLECKSIRYNTLSNNKIIS
                     KVALCGGSGSFLIEEAVKLNADAFVTSDLKYHNFLDSAIENMLLIDAGHYETEIGGLN
                     LLKEFLLQKFPNFAVEISQKGLNPVNYL"
BASE COUNT          380 a          221 c          200 g          392 t
ORIGIN      
        1 gattgaaagt tcgacattct cttctgtttg tggattctga aggttggcgt ttgtgtccat
       61 aaatttattt tcctgattaa gatattacag tgcaacttat tatatcacaa ataattaaca
      121 ggattcaatc ctttttgtga aatttccact gcaaaattag gaaatttttg taagagaaat
      181 tctttcagaa ggttgaggcc tccaatttct gtttcataat gacctgcatc aataagtagc
      241 atattttcaa ttgctgaatc gagaaaattg tgatatttga gatctgaagt tacaaaagcg
      301 tcggcattca attttactgc ttcttcgatg agaaacgaac cagaacctcc acataaggca
      361 actttagaaa ttatcttgtt gttgcttaag gtattatatc gaatcgactt acattctaat
      421 gatgatttta gaaaagccaa aaaatccata agttcaaaat tctgaggtaa atttccgata
      481 atcccctctc ctactgatgt ataaacatta tctaaaggaa taatatcata agcaacttct
      541 tcatagggat gaacttccaa taataatcgt attatcgtgt cggttaggtg aactgggaaa
      601 atcacttcta tacgagtttc caattcctta tgtatctgat tgatttctcc cacgtatgga
      661 tgtgaaccct cctgagctcg aaaagtacca tatccttcgg cattgaagct gcaactatcg
      721 taatttccaa tatatcctcc accgacactg aataaagccg aacgaacttt ttccgaaaaa
      781 ccaactggac aaaatgtaac caactttctc aaagctcctt tcttaggaca taaaatcata
      841 gtatctttta atccgagttt gttagcaagt gcttcattcg ttccttttgc cacattatcg
      901 aaattagtat gaaaggaaat gagagaaatt ctattttcgg taagttttcg tatgattctt
      961 ccttcggact ttgaaaagtc gagataacga aggcctttga agaaaaaagg atgatgagta
     1021 agaatcagat tgaatcgatt ttgtatggct tcatcaataa tttctgtagt cagatcgagg
     1081 caaagaagta ttccatgaac ctcctcatcc ttatccccca actgaaatcc tgaattgtca
     1141 tattcttctt gatagacgaa tggtgcatat tggtcgagca cttgcaatac atc
//
LOCUS       DAORPD010000681         1192 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_24702_length_1192_cov_2.598065, whole genome shotgun sequence.
ACCESSION   DAORPD010000681 DAORPD010000000
VERSION     DAORPD010000681.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24702_length_1192_cov_2.598065"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1192
                     /gene="rseP"
                     /locus_tag="PK976_07810"
     CDS             <1..>1192
                     /gene="rseP"
                     /locus_tag="PK976_07810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HRC67831.1"
                     /translation="YLFFDAGNFKLFSFKKGETEYGVGWLPLGGYVKIAGMIDESMDK
                     EQLKSPPQPWEFRSKPAWQRLIVMLAGVFVNLILGILIFGFTLLHYEKSYLPLNEINK
                     DGIYASAKGKEIGFEAGDKILRINGKPVERFKDVHSVDLLFGSTFEVERNGKIITIEI
                     PEDFYRREASSRGVRFIEPLNFPFRIDSVFPGLPAANAGLTKSDKILKIDSIEIKSYG
                     QFKELITNSAGRLVAIQTLRGNDTLNMQLTVDSSGIIGILASIPYAQAPYSFGKALSY
                     GYKDAMDMLSANIKGLGKIISGEEKASDSLQGPIGIAQIYGSQWIWAKFWYITGLLSL
                     ILAFMNILPIPALDGGHVLFLLIEIVTRRKPSEKVLEYAQIAGMIILLGIMVFAIGND
                     IMRIL"
BASE COUNT          335 a          267 c          254 g          336 t
ORIGIN      
        1 tttatttgtt ttttgacgcc ggaaacttca aattattcag tttcaagaaa ggggaaaccg
       61 agtatggtgt gggatggctt cctttgggtg gctatgtaaa gattgccggc atgatcgacg
      121 aatcaatgga taaggaacaa ctgaaatctc ctccacaacc ctgggaattt cgttcaaaac
      181 ctgcctggca gcgcctcatt gtaatgctcg caggtgtttt cgtcaatctt atcttgggta
      241 tcctcatatt tggcttcaca cttttacatt atgaaaaatc atatctccct ttgaatgaaa
      301 taaataaaga tgggatatat gcctcggcaa aaggcaaaga aattggtttc gaggccggcg
      361 acaaaatcct acgcatcaat ggcaaacccg ttgagcgctt caaagatgta cattcagtcg
      421 acttgctctt cggcagcact ttcgaagtag aacgaaacgg taaaattatt accatcgaga
      481 tcccggaaga tttctaccgt cgtgaagcca gtagccgtgg cgtaagattc atcgaacctc
      541 tcaactttcc gtttcgtata gatagcgttt tccccggatt gcctgcggca aatgcaggtt
      601 taacaaagtc tgacaagatt ctcaaaatcg actctataga aataaaatct tacgggcaat
      661 tcaaggaact gatcactaat tctgcaggtc gtctggtagc aattcaaaca ctaagaggaa
      721 acgacactct gaacatgcaa ctcacagtcg attcatcggg tattattggt attttggctt
      781 ccatacctta tgcacaggca ccttatagtt ttgggaaagc cctttcttat ggctataagg
      841 acgctatgga tatgctttcg gccaatatca agggccttgg gaaaatcatc agcggtgagg
      901 aaaaagccag cgacagcctt caagggccta ttggcatagc ccaaatctat ggctctcaat
      961 ggatctgggc aaaattttgg tacattacag gcctgctttc gcttatcctc gccttcatga
     1021 atatcctccc tatccccgct ctcgatggcg gacatgtact tttcttgctc atcgaaatag
     1081 taactcgccg caaacccagc gaaaaagtcc tcgaatatgc tcaaatcgct ggtatgatta
     1141 ttcttttggg gattatggta tttgctatag gtaacgacat catgagaatc ct
//
LOCUS       DAORPD010000682         1189 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_24809_length_1189_cov_2.747795, whole genome shotgun sequence.
ACCESSION   DAORPD010000682 DAORPD010000000
VERSION     DAORPD010000682.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24809_length_1189_cov_2.747795"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..1060)
                     /locus_tag="PK976_07815"
     CDS             complement(176..1060)
                     /locus_tag="PK976_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014775862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRC67832.1"
                     /translation="MKQAVALVLSSGGARGLAHIGVIEELEQAGFEIKSVAGTSMGAL
                     VGGIFATGKLKECTEWICSLDKKSVLSMFDFTISSTGLVKGQKVLDQIQQRIPDLPIE
                     DLLVHFCAVATDIKNQKENVFEKGSLFEAIRASISIPGVFIPYKSDGKILVDGGVVNP
                     LPLNHVKRPENSILVAVDVNAAHALHHSKRKKIRESQNNHELKFLELLRTKSKNLKLQ
                     KNEEEQYNYYQLLTLSAQMMLQRITNLTLAIYPPDMLIRIPMDSFGTFHFYKAKEIIE
                     SGRNAAKKALEEYRKNHC"
BASE COUNT          338 a          243 c          200 g          408 t
ORIGIN      
        1 ttaacaattt tcagtttata gacagactct aattaaaagc aaatcagtag ttctgagatt
       61 ttttattcaa tctttagcta aggaggctat tttgaattag ccacataaac ttacatctgt
      121 ggaagacaag atttcggttt tgacccatct tctttttatt cgattttttt gtcggtcagc
      181 agtgattctt tctgtattct tccaaggcct tttttgcggc atttcttcct gattcaataa
      241 tttcttttgc cttgtagaaa tgaaaggttc caaatgaatc cattgggatc cgaataagca
      301 tatcgggagg ataaatcgcc agagttagat tggtaattct ctgaagcatc atctgagccg
      361 aaagggtaag taattgataa taattatatt gttcttcttc atttttttga agtttgagat
      421 ttttcgattt tgttcttaaa agttcaagaa atttcagttc atgattattt tggctttctc
      481 gaattttttt ccgcttcgaa tgatgtaaag catgcgcagc gtttacatca acggcaacca
      541 gaatcgaatt ctcgggtcgt ttcacatggt ttagcggcaa aggattaaca actccaccat
      601 ccacaagtat tttcccatcc gatttgtaag ggataaacac acccggaatg gaaatggaag
      661 ctctaatggc ttcaaaaagg cttcctttct caaaaacatt ttctttttga tttttaatat
      721 cggtggccac agcacaaaaa tgtaccagta aatcttctat gggcaaatcg ggaatccttt
      781 gctgaatttg gtcgaggact ttttgacctt ttaccagtcc cgtcgagctt atcgtaaagt
      841 cgaacatact caaaaccgat tttttatcga gcgaacaaat ccattctgta cattctttaa
      901 gtttgcccgt ggcaaaaatt ccccccacca gtgcacccat cgaagtgccc gcaaccgact
      961 ttatctcaaa acctgcctgc tcgagttcct ctatgactcc tatatgagcc aatcctcttg
     1021 ctcctccgct cgataatacc aatgccactg cttgtttcat tccccgaaaa ataaagttct
     1081 ttctttctat ttattatgtg acttcctaac tcttgaaaat tattgaagct tattagaaac
     1141 gacaaagtta tataagattc gcacagtggt aattcataaa tagatatat
//
LOCUS       DAORPD010000683         1189 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_24811_length_1189_cov_2.705467, whole genome shotgun sequence.
ACCESSION   DAORPD010000683 DAORPD010000000
VERSION     DAORPD010000683.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24811_length_1189_cov_2.705467"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /gene="glnS"
                     /locus_tag="PK976_07820"
     CDS             complement(<1..920)
                     /gene="glnS"
                     /locus_tag="PK976_07820"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HRC67833.1"
                     /translation="MNDRRTNHFEENSPNEPDKTGLNFIEEIIEEDLRKGKNRGRVHT
                     RFPPEPNGYLHIGHAKSICLNFGLAQKYGGFTNLRFDDTNPTKEGEEYVDAIKEDIRW
                     LGFSWDDRDYYASDYFDQLYEWAEKLILEGKAYVDDQSAEEISRQRGTITTPGIESPY
                     RNRSVEENLNLFRRMRAGEFPDGHCVLRAKIDMSHPNMLMRDPIMYRIIHTSHHRTGD
                     KWCIYPMYDYAHGQSDYIEGITHSICTLEFEIHRPLYDWFLDQIVTTEYRPRQIEFAR
                     LNLSYTVLSKRKLLKLVKEGFVSGWDDPRMP"
BASE COUNT          303 a          251 c          270 g          365 t
ORIGIN      
        1 ggcatgcgcg gatcgtccca gccgctgaca aagccttcct tgaccagttt gaggagcttg
       61 cgcttgctga gcaccgtgta gctcaggttc aggcgggcga actcgatttg tcgaggccga
      121 tattcagtag tgactatctg atctaagaac caatcgtata aagggcgatg aatctcgaat
      181 tcgagtgtgc agatggaatg agttatgcct tcgatgtagt cgctttgacc atgtgcatag
      241 tcatacatgg gatatatgca ccatttgtca cctgttcgat gatgggaagt atgaatgatc
      301 cgatacatga taggatcgcg catgagcatg ttgggatgcg acatgtcgat ttttgccctc
      361 aaaacacaat gtccatcagg aaattcacct gccctcattc ggcgaaacag gtttaaattc
      421 tcttccacac tacggtttct gtaagggctt tctatcccag gtgtcgtgat cgttcctctc
      481 tgacggctga tttcttcggc cgattgatca tccacataag ctttcccttc aaggatgagt
      541 ttttcggccc attcatatag ctgatcgaaa tagtcagaag cataataatc tctatcgtcc
      601 catgaaaatc caagccagcg aatgtcttcc ttaatggcat ccacgtattc ttcgccttct
      661 ttggtgggat tggtatcgtc gaaacggaga ttggtaaacc ctccgtattt ttgagccaac
      721 ccaaagttaa gacaaatgga tttagcatgc ccaatatgaa ggtacccgtt gggttcgggt
      781 ggaaagcgcg tatgcactcg gcctcgattt ttccctttgc gtaaatcttc ttctatgatt
      841 tcttctataa agttcaatcc tgttttgtct ggctcgttag gagaattctc ctcgaaatga
      901 ttcgtcctgc ggtcgttcat ccaaaaattg ttgttttgat ggctgtaaag ctttgtacga
      961 gtggaagtaa aactatagat ttcgcttgca aaagtaatta tacttgaagg attggacaag
     1021 gaaattacaa tggaataaaa tatatcttat agatattcaa tagattatga ttataacgca
     1081 aagtatgatt ttgttaatcg agtggtttgt cctaattttg tccttccata tttaaggtct
     1141 cgtggccgag tggctaggca caggtctgca aaacctcgta cagcggttc
//
LOCUS       DAORPD010000684         1188 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_24853_length_1188_cov_2.718447, whole genome shotgun sequence.
ACCESSION   DAORPD010000684 DAORPD010000000
VERSION     DAORPD010000684.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24853_length_1188_cov_2.718447"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1188
                     /locus_tag="PK976_07825"
     CDS             <1..>1188
                     /locus_tag="PK976_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016695 - oxidoreductase activity, acting
                     on hydrogen as donor [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="[FeFe] hydrogenase, group A"
                     /protein_id="HRC67834.1"
                     /translation="QGEQSTYRKDTSPSIIRDVDKCILCRRCEMMCNDIQTVGALSAI
                     NRGFMAVVAPAFEMNLEKSPCTYCGQCVAVCPTGALTEVDNTRQVISALADPTKTVIV
                     QTAPAVRAALGEEFGLEPGSLVTGKMVSALRELGFDYVFDTDFAADLTIMEEGSELLD
                     RLTRYLSGDRNVRLPILTSCCPAWVNFFEHNFPELKDVPSTARSPQQMFGSIAKTYFA
                     KKLGIDRNNLVVVSIMPCLAKKYECQRSEFLTDGNPDVDFSLSTRELANFIKLANIDF
                     NSLPESDFDRPLGESTGAAVIFGTTGGVIEAAVRTAYELYTKKPLPRVDFEELRGLEG
                     VRAATVNFDGLELRIGIAHGLGNARQLLEEVRDGKSQYHAIEIMACPGGCIGGGGQPL
                     HHG"
BASE COUNT          310 a          259 c          300 g          319 t
ORIGIN      
        1 atcaaggcga acaatctact taccgcaagg atacatctcc aagcataatt cgcgatgtgg
       61 ataaatgcat cctgtgcaga cggtgcgaaa tgatgtgcaa cgatattcaa accgtaggtg
      121 ccctttcggc cattaacaga gggtttatgg cagttgtagc accggctttc gaaatgaatc
      181 tcgaaaaatc tccatgtacc tattgtggcc aatgtgtagc agtttgtcca acgggtgcac
      241 ttaccgaggt ggacaacacc cgtcaggtaa tttcggcttt ggctgatcca accaaaactg
      301 tgatagtaca aactgctccg gctgttaggg cggctttagg agaagaattt ggcctcgaac
      361 ccggatccct ggtgacaggg aaaatggttt ccgcactgcg cgaattagga ttcgattatg
      421 ttttcgacac cgatttcgcc gctgatctta cgattatgga agaaggcagt gagctcctcg
      481 accgtctcac cagatattta tcaggcgaca gaaatgtacg gcttcctata cttacttctt
      541 gttgtcctgc atgggtaaat ttttttgaac ataatttccc tgaattgaag gatgttcctt
      601 ccacggcacg ttcacctcag caaatgtttg gttcaatagc aaaaacttat ttcgccaaaa
      661 aactgggcat cgatcgtaat aatctggtag tagtatccat tatgccttgt ttggctaaga
      721 aatatgaatg ccagaggtca gaatttttga ccgatggaaa tcccgatgtt gatttttcgc
      781 tttctactcg tgaactggct aatttcatta aactggccaa tattgatttc aactcgcttc
      841 ccgaaagcga tttcgaccgt cctttggggg aatcgacggg agctgcagtc atttttggaa
      901 ccactggagg agtgatcgaa gcggccgtta gaacagctta tgaactttat acgaaaaagc
      961 ctttacccag agtcgacttc gaggagctga gagggctcga aggagtgaga gccgctaccg
     1021 tgaatttcga cggtttggaa ctgcgtatag gcattgccca tggattgggg aatgcacgcc
     1081 aactgctcga ggaagtaaga gacggtaagt cgcaatatca tgccatcgag atcatggctt
     1141 gcccgggcgg atgtattgga gggggggggc aacctcttca tcatggtg
//
LOCUS       DAORPD010000685         1186 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_24933_length_1186_cov_2.263484, whole genome shotgun sequence.
ACCESSION   DAORPD010000685 DAORPD010000000
VERSION     DAORPD010000685.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24933_length_1186_cov_2.263484"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="PK976_07830"
     CDS             complement(<1..225)
                     /locus_tag="PK976_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67835.1"
                     /translation="MEVKKNIISKGTIKGKFLGFKNNYTIFEFTDGQKWRQNESKFWH
                     HFAINPEAEISYHDGKYYLGIKGLEKTVEVR"
     gene            complement(452..>1186)
                     /locus_tag="PK976_07835"
     CDS             complement(452..>1186)
                     /locus_tag="PK976_07835"
                     /inference="COORDINATES: protein motif:HMM:NF024246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRC67836.1"
                     /translation="KSMDNEFSLMFEEYLPYFRLAHKQFRVIPVDSSIGALNSVAPYN
                     KIKELVKNQDLISLTECICRKEQKLVGKGCDSPKETCLAFGTLAQFYIDNKIGRIITA
                     EEALKVLDMAEEAGLVLSPTNAQQLEFVCCCCTCCCAYLRAAKHSSKASRMITSYYQS
                     KINPGLCSFCGLCIERCPMDAIAENDECCEIIEEKCIGCGLCVGVCPEGAITLVAKSG
                     VEPPPLDFDETLSKIETERRSKKSLS"
BASE COUNT          327 a          252 c          228 g          379 t
ORIGIN      
        1 tctgacctca acggtctttt cgaggccttt gatccccaag taatattttc catcatgata
       61 gctgatttcc gcttctggat taatggcgaa gtgatgccag aacttgcttt cgttttggcg
      121 ccatttctgg ccgtcggtaa actcaaatat tgtgtaattg tttttgaaac ccaggaattt
      181 accttttatt gtacctttag atataatatt tttttttact tccatcaggg tagcctttat
      241 gccaaaaaaa caaagcacac aacgaagttt aatttttata taccttatat agatgcgatt
      301 tgtcatgctg aatttgtttt gaacaaatat tattgtttat atattccaag tagcgctcca
      361 aatctgttct tttgaggata tcccttacgg attctacagg aaaatcaggc attcgaatat
      421 agagttggaa ttcaatatcc tactcagaat atcaggatag ggactttttg ctgcggcgct
      481 cggtctcgat tttcgaaaga gtctcgtcaa agtccaaagg agggggttcc actcctgatt
      541 tggctactaa agtaatagcc ccttctgggc aaacaccaac acataatccg cagccaatac
      601 atttttcttc tataatttcg caacattcat cattttctgc gattgcgtcc attggacatc
      661 gctcgatgca taaaccgcag aacgaacaca aaccgggatt aattttagac tggtaatacg
      721 atgtaatcat acgactggct tttgaagaat gtttggctgc tctgagataa gcgcaacaac
      781 acgtacaaca gcaacaaaca aattctaact gctgagcgtt ggtaggggag agcactaacc
      841 cggcctcttc ggccatgtcc agaaccttca gtgcttcttc tgccgtgatt atccttccta
      901 tcttgttgtc gatatagaat tgggcaagtg ttccgaatgc aaggcatgtt tctttggggc
      961 tatcacaccc ttttcccact agtttttgtt ctttacggca aatgcattct gtcagagaaa
     1021 tcaaatcctg atttttgact aattccttta tcttgttata aggagcaacg ctattgaggg
     1081 caccgataga cgagtcgacc ggaatgacac gaaactgttt gtgagccaac ctgaaataag
     1141 gcaggtattc ttcgaacata agtgaaaatt cattatccat gctttt
//
LOCUS       DAORPD010000686         1184 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_24991_length_1184_cov_2.921169, whole genome shotgun sequence.
ACCESSION   DAORPD010000686 DAORPD010000000
VERSION     DAORPD010000686.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24991_length_1184_cov_2.921169"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1050
                     /locus_tag="PK976_07840"
     CDS             <1..1050
                     /locus_tag="PK976_07840"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRC67837.1"
                     /translation="GDTLKLGNELFYEGKSSGQFIIPNASNEGCDSIVFVNILFNTPS
                     RIDTLRLAAMQESEIFTFGEFEFSIDKKEGLVKLSETNVLGCDSLIYVSVSFPTDFRN
                     LNWKVLPATCLEQNNGSFTLINAEGCEPEKLTINNVVYDIDKLPFDVLNLSPGVYNWK
                     LESNAKCNSAGEFIIEGISAVGFEVEPTTVEVPIRSETLVNFLVSPEPFSAQWLDASG
                     LSCNDCLSPIIEAPGSLLKYTLLLTDTNGCESKYNITVIPLETIKVWPNIIYPDGGGI
                     NSVFNVANEDNTLNAVFIYDRWGNALVSKNNFGNGESFTWDGTAANNRVSQGLYLISI
                     QYKDGSTEWVEVTVL"
BASE COUNT          339 a          219 c          259 g          367 t
ORIGIN      
        1 ggtgataccc tcaaattggg gaatgaattg ttttatgagg gcaaaagttc gggacagttc
       61 attataccta atgcatctaa tgaaggttgt gattctatcg tctttgtaaa cattcttttc
      121 aatacacctt ctcgaatcga cacccttaga ttagccgcta tgcaagaatc agaaattttt
      181 acattcggtg aatttgaatt ttctattgac aaaaaggaag gtttggtgaa attatctgaa
      241 accaatgttc taggttgtga ttcgttaatt tacgtatctg ttagctttcc taccgacttt
      301 cgcaatttaa attggaaggt attaccagct acatgtttag aacaaaataa tggaagtttt
      361 acactaatca atgctgaagg atgtgaaccg gaaaaattga ccattaataa tgttgtgtat
      421 gatatcgaca aattgccatt tgatgtattg aacttaagcc ccggtgtata caactggaaa
      481 ctggaaagta atgcaaagtg caattctgcg ggtgaattta ttatcgaagg aattagtgca
      541 gttggatttg aagttgaacc aaccaccgtt gaagttccca taagatcgga aacgctcgtg
      601 aattttttgg taagtccgga acctttttca gctcaatggt tggatgccag tggcttaagt
      661 tgcaacgatt gtttatcacc catcatagaa gcaccgggtt cattgcttaa gtatacctta
      721 ttgctgacag atacaaacgg ctgtgaatcg aaatacaata ttaccgttat ccctctcgaa
      781 accataaaag tatggcccaa tatcatttat ccggatggag gaggcatcaa cagtgtcttt
      841 aatgtggcca atgaggacaa tacgcttaat gctgttttta tttacgatcg atggggcaac
      901 gccctcgtga gtaaaaacaa ttttggaaat ggagaatcat ttacctggga tggaacagca
      961 gccaataata gagtaagtca agggctttat cttatttcca tccagtataa agatggaagc
     1021 actgagtggg ttgaggtcac ggttttgtaa atgttcacgc ctccttgcag tcagctaaat
     1081 gtttgcgcct ccctttggtc ggctaaatgg tttcgcctcc cggtggtcgg ctaaatgttt
     1141 gcgcctccct ttggtcggct aaatggtttc gcctcccggt ggtc
//
LOCUS       DAORPD010000687         1173 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_25402_length_1173_cov_1.940966, whole genome shotgun sequence.
ACCESSION   DAORPD010000687 DAORPD010000000
VERSION     DAORPD010000687.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25402_length_1173_cov_1.940966"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="PK976_07845"
     CDS             complement(<1..849)
                     /locus_tag="PK976_07845"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HRC67838.1"
                     /translation="MLKSLKVLRYAIPYWSSALLNIIFNLLSVIFSLVSFALFIPVLQ
                     IIFDRTKRLVPGVDFNPDWHSIHDTDSLKQFFYGSTSQMIEIQGEMKALLYISILIVV
                     LYLLKNLFRYLAMFFLATVRNGVVKDLRNALYYKALILPLSFYTQQRKGDLMARMGGD
                     VQEVEWSIMSSLEMLFRDPITIIVYLITLFIIQPVLTGFVLVLLPISGFLIAQVGKSL
                     KRTSQKSQKKMGYLMSLIEETLSGIRIIKAFNAIDESREHFEKENHAYTRMMTRLYRK
                     RDLASPL"
BASE COUNT          387 a          229 c          214 g          343 t
ORIGIN      
        1 caagggtgaa gccaaatcac gcttacgata caatcgggtc atcatacgtg tgtaggcatg
       61 attttccttc tcgaaatgtt cccgtgattc atctattgca ttaaaagcct taatgatacg
      121 aattccagat agggtttctt caatcaacga catgagatat cccatttttt tctggctttt
      181 ttgggatgtt cgttttaagc ttttcccaac ctgagcaatt aaaaatccgc tgatgggaag
      241 caaaaccagt acgaaacccg tcagcaccgg ctgaataata aaaagcgtta ttaaataaac
      301 gataatggta atgggatcgc gaaacaacat ttcgagcgag ctcatgatcg accattccac
      361 ttcctgaaca tcgcccccca ttctggccat taaatcaccc ttgcgctgct gagtgtagaa
      421 cgataaggga agaatcaagg ccttgtaata taacgcattg cgaagatcct tcacgacccc
      481 gttgcgtacc gtggctaaaa aaaacatggc cagatagcga aacagattct ttaaaaggta
      541 taaaaccaca atgagaatgg aaatatatag aagggccttc atctcaccct gaatctctat
      601 catctggctc gtactgccgt aaaaaaactg cttgagtgaa tccgtgtcat gaatactatg
      661 ccaatcggga ttgaaatcaa caccaggcac caatcttttg gttctgtcga aaataatctg
      721 taatacgggt atgaacagag caaatgatac caaactaaaa attacggata aaagattgaa
      781 tatgatatta agcaaagccg aactccaata tggaatcgca taacgaagaa cttttaggct
      841 tttcaacatt gtgcttataa taataaattg caaaggtaaa atgaactttc attcatagat
      901 gaacctttca ttttaattgc cagactggcg ggttctggga aagaaaacag ggaatgtcga
      961 tggaattttc atgatgcctc tttgccgatg tccaaaataa acacctggat ttacagctct
     1021 tcctaagctt tgcttatatt cataaaataa ttatcttttg atcttttttt actgaattat
     1081 ggaattttga cgtatctttg caaaaaatta aggttactaa gttcgtatta ttttatttta
     1141 ataatttaat tttcagatga ctgaaaacaa gcc
//
LOCUS       DAORPD010000688         1172 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_25431_length_1172_cov_2.617726, whole genome shotgun sequence.
ACCESSION   DAORPD010000688 DAORPD010000000
VERSION     DAORPD010000688.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25431_length_1172_cov_2.617726"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="PK976_07850"
     CDS             complement(<1..598)
                     /locus_tag="PK976_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67839.1"
                     /translation="MQNKPLSDIHVMVSMLHSDNIVGFDITTDEGQFRIHLRCVGDSV
                     RIKTSSVSHEDFKVMIACKSQHLDIILKESVKDLKEITVKARHVIKKGDTLSYLVKPF
                     AREQDKTIAEVIRRIPGIEVETSGRILYEGTPISNLYVEGMDLMEDRYSLLTDNLPYD
                     KVSTVEVITNHEPVKILQKYSTSGKVALNLKLRNKTTLT"
     gene            complement(710..>1172)
                     /locus_tag="PK976_07855"
     CDS             complement(710..>1172)
                     /locus_tag="PK976_07855"
                     /inference="COORDINATES: protein motif:HMM:TIGR01200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GLPGLI family protein"
                     /protein_id="HRC67840.1"
                     /translation="YDYILMSGHYRYEETPHLIWKLESDTANILGYTTQHAWTYYGGR
                     IWHAWFTSAIPISDGPYKFMGLPGLILKVEDSKQHYIFEAIAIEQPAEGENITYEENQ
                     YIETTREKFLEANRNLRLDIINRAQEAGLDAGAQQTAARNMRSHNNPIELK"
BASE COUNT          325 a          252 c          231 g          364 t
ORIGIN      
        1 ctgtgagggt tgttttattc cttaatttca gattgagagc aaccttcccc gacgttgaat
       61 atttctgaag aatttttact ggctcatgat tagtaatcac ctcaacggta gaaaccttat
      121 cataaggcag attgtccgtt agaagagaat aacggtcttc catcaaatcc atgccttcta
      181 cataaagatt cgatataggt gtaccctcgt aaagtatcct gccagatgtc tccacttcaa
      241 ttcccggaat acgacgtatt acctcggcaa tagtcttatc ctgttcgcgt gcaaaaggtt
      301 ttaccaaata actcaaggta tcgccttttt taatgacgtg cctggctttt acggtaatct
      361 ccttgaggtc tttcaccgac tctttcagaa tgatatcaag atgctgactt ttacatgcta
      421 tcataacctt gaaatcttca tgactgaccg aagaggtttt tatacgtaca gaatctccca
      481 cacaacgcag atggatacga aattgtccct catcagtggt aatatcgaaa ccaacgatat
      541 tatcggaatg taacatagaa accattacat gaatatcgct caagggttta ttttgcacgt
      601 ccttgacttt cccttcgatt attacctgcg aaaaaagagg taaaaaagtc gttagacaaa
      661 ataagaatat aataagcctg cgcattttca taatgtggag gaaggcttat tatttaagct
      721 ctattggatt gttatgcgaa cgcatatttc gagctgcagt ttgctgtgct ccggcatcca
      781 aaccagcttc ctgtgccctg ttgatgatgt cgagacgtaa attgcgattg gcttccagaa
      841 atttctctct ggtggtttct atgtattgat tctcttcgta tgtaatattt tctccctcgg
      901 ctggctgttc tatggcaata gcctcgaata tataatgctg tttcgaatcc tcgaccttta
      961 gaatgaggcc gggtaaaccc atgaatttat agggaccatc gctgatggga atggcagacg
     1021 taaaccatgc atgccaaatg cgaccgccat aatatgtcca ggcatgctgt gtggtatatc
     1081 caagaatatt ggccgtatcc gactcgagtt tccagatcag atgtggagtt tcttcatacc
     1141 tgtaatgccc gctcatcaaa atataatcgt ag
//
LOCUS       DAORPD010000689         1171 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_25474_length_1171_cov_2.497312, whole genome shotgun sequence.
ACCESSION   DAORPD010000689 DAORPD010000000
VERSION     DAORPD010000689.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25474_length_1171_cov_2.497312"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1074)
                     /locus_tag="PK976_07860"
     CDS             complement(<1..1074)
                     /locus_tag="PK976_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67841.1"
                     /translation="MSVSTASVSPYYNPWFSQDGEKLYFAYYYPPEESKTDTLHLPEE
                     IARLDIWSWSDPLLQSEQLKNLENEKKRSYVCMYDIKKEKIILAGRDTISQISIYMKG
                     NSRYALGSNPLPYSQLKSWDGSYYDYYLIDLQNGKAKLIERKVADYVSLSPYGYFYIR
                     YHSNDSCWYATNLKNNECIPLTKGLSACFALEEYDMPSPPPSYGIAGWATNDEFVLIY
                     DKYDIWKIDPKGKQAPENLTRFYGRKNKLKLRYLKTNPEEIHIDLEKPLLLAGYSYID
                     KDIYILTLSPRLKREPSILTGGKCRISNLIKSRNSDTFLFTKETFQQFPDLWYAQNDF
                     NIMEQISQANPQQSEYQWGTVEVV"
BASE COUNT          328 a          212 c          238 g          393 t
ORIGIN      
        1 caccacttcc acagttcccc attgatattc tgattgctgt ggattggctt gtgaaatctg
       61 ctccataata ttaaagtcgt tttgggcata ccatagatcg gggaattgtt gaaatgtttc
      121 ctttgtaaat agaaaagtgt ctgaattcct tgattttatc aaattgctta tccggcattt
      181 gcctcctgtt aatatggaag gttcacgttt taatcgagga gaaagtgtta aaatgtatat
      241 gtctttatcg atatagctat acccggcaag caatagaggc ttttcgaggt caatatgaat
      301 ttcttctggg ttggttttca aatacctcaa ttttaattta ttttttcttc catagaagcg
      361 agtgagattt tcgggagctt gtttcccctt aggatctatt ttccatatat catatttatc
      421 gtaaatcaac acaaattcgt cattagttgc ccatcctgca ataccatagg agggaggagg
      481 ggaaggcatg tcgtattctt cgagagcgaa acatgctgac aaccctttgg taagcgggat
      541 gcattcgttg ttctttaaat ttgtggcata ccagcatgaa tcattcgaat gatatcgaat
      601 ataaaagtag ccataaggcg aaaggctgac ataatcggca acctttcttt cgatgagttt
      661 tgccttgcca ttctgtaaat ctattaaata gtagtcataa tagctgccat cccacgattt
      721 cagctgagaa taggggaggg gattgcttcc cagagcgtat ctcgagtttc ccttcatata
      781 aatggaaatt tgagagatgg tgtcccttcc tgctaaaatt atcttttctt ttttgatatc
      841 atacatgcaa acataggagc gcttcttttc attttcgaga tttttgagtt gttctgattg
      901 cagcaaggga tcactccagc tccagatgtc caaccttgca atttcttcgg gaagatgaag
      961 cgtgtctgtt ttagactctt ctggtggata gtaatatgcg aaatacaatt tttctccatc
     1021 ttgagaaaac cagggattgt aatatggact aacgctggca gtagaaactg acaaaattgc
     1081 agaatctgct ataagttgac tctcattttt atttactcca tacaagtata agcaaatatt
     1141 cgagcgaaaa gaagtgtctg ttgaatatga g
//
LOCUS       DAORPD010000690         1171 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_25479_length_1171_cov_2.096774, whole genome shotgun sequence.
ACCESSION   DAORPD010000690 DAORPD010000000
VERSION     DAORPD010000690.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25479_length_1171_cov_2.096774"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            160..>1171
                     /locus_tag="PK976_07865"
     CDS             160..>1171
                     /locus_tag="PK976_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006283814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRC67842.1"
                     /translation="MEHLKKDMYLDMELLRFTTAGSVDDGKSTLIGRLLYDSKSIFED
                     QLEAVKNVSNQKGDHYLDLALFTDGLRSERQQGITIDVAYRYFYTPKRKFIIADTPGH
                     IQYTRNMVTGASTANAAVILVDARNGITEQTLRHYYIAYLLGIKSIIVCINKMDLVNY
                     SSEPYFLIQEEIIRISQKLPVANLIFIPISALKGDNVVKPSANTPWYSGQTLMQTLEA
                     IPIYNHSHRGPLRVPVQYVIRPMLDEFHDYRGYAGRIESGTIRKGDKVMIMPSYMLSE
                     VTSVHLGENFLEEAYAPLSVALTLKDDLDLDRGCFICQPEQLPQTSSILEVMICWFNM
                     IPM"
BASE COUNT          337 a          266 c          246 g          322 t
ORIGIN      
        1 tgtacggggc tttccctgtc gaaagccagt agtttggagg atattatctc agagattgcc
       61 tcttcgagag ttaccgaaag aggctcacgc tgggacgaca agcgttcgga tgctgccatg
      121 gaagaccgaa aacgcgaggg ctatttttaa ttttacgcta tggaacactt aaagaaagat
      181 atgtatcttg acatggaact gcttcgtttt acgacggcag gcagtgtaga tgatgggaaa
      241 agtactctca tagggcgctt attatacgac agtaaatcta ttttcgaaga tcagctggaa
      301 gccgttaaaa atgttagcaa ccagaaaggc gatcattacc tcgatttggc tctctttact
      361 gatggtctgc gatccgaacg ccaacaagga attactattg atgtagctta tcgttatttt
      421 tataccccca aacgcaaatt catcatagcc gatacaccag gccatataca atatacacgc
      481 aacatggtaa caggagcatc aaccgctaat gctgccgtta tactcgtcga tgccagaaat
      541 ggcatcaccg aacaaactct ccgacattac tatatcgcct accttctggg tataaaaagc
      601 ataatcgtat gcatcaataa gatggacctg gtaaattatt cgtctgagcc ttatttcctg
      661 attcaagaag aaataatacg tatcagccag aaattacctg ttgctaacct gatttttatc
      721 cccatcagtg cactaaaggg agacaatgta gtaaaacctt ctgccaacac cccctggtat
      781 tccggtcaaa ccctgatgca aaccttggaa gctattccca tatacaatca ttctcatcgg
      841 gggcccttga gagttcctgt gcagtatgtc atccggccta tgctcgatga atttcatgat
      901 taccgtgggt acgccggaag aatagaatct ggtactatca gaaaaggtga taaagttatg
      961 attatgccat cgtacatgtt gtcggaggta acctctgttc atttaggcga aaactttctc
     1021 gaagaagctt atgctccgct ttcggtggca cttacgctca aagatgatct cgatctcgat
     1081 aggggctgct tcatctgcca acccgagcaa ctaccccaaa cttcctctat tctcgaggtt
     1141 atgatatgtt ggttcaatat gatacctatg a
//
LOCUS       DAORPD010000691         1169 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_25561_length_1169_cov_2.392280, whole genome shotgun sequence.
ACCESSION   DAORPD010000691 DAORPD010000000
VERSION     DAORPD010000691.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25561_length_1169_cov_2.392280"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1169)
                     /locus_tag="PK976_07870"
     CDS             complement(<1..>1169)
                     /locus_tag="PK976_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HRC67843.1"
                     /translation="IPNMYKMAGELLPAVFHVSARTIATHALSIFGDHSDVMATRQTG
                     FALLASGSVQEIMDIAPVAHLTTVKARIPFLHFFDGFRTSHEIQKIEVFGEAEVASLL
                     DKQALAAFRSRALNPEHPVTRGTAQNPDIFFQAREASNRFYDAVPDLVEHYMNEMTRI
                     TGREHHPFDYYGALDAENIIIAMGSVTETIKEVIDYKLKQGEKIGLICVHLYRPFSSK
                     YFFKVLPSSVKRIAVLDRTKEPGADGEPLYQDIRSMFYGMDNTPLIVGGRYGLSSKDT
                     TPSQILAVYDNLRMPEPKNDFTIGIVDDVTFKSLPLLPEQSFAPEGTYEAKFYGLGSD
                     GTVGANKNTIKIIGEETDLYCQAYFAYDSKKSGGFTASHLRFGKHPIRSTYLVTN"
BASE COUNT          329 a          236 c          279 g          325 t
ORIGIN      
        1 gattggtaac cagataggta gaacgaatgg ggtgctttcc aaatcgtaga tgggaggctg
       61 taaatccacc tgatttttta gagtcgtagg caaaataggc ttggcaatat aaatcggttt
      121 cttcaccaat aattttgata gtgttcttgt tagctccaac tgtaccgtcc gagcctaacc
      181 catagaattt agcttcataa gttccttctg gtgcaaaact ttgctctggg agcaaaggca
      241 acgatttaaa tgtaacatca tctacgatgc caatcgtaaa atcgttttta ggttcgggca
      301 tccgtagatt atcgtaaacg gcaagaattt gagaaggtgt agtgtctttc gagctcaatc
      361 catagcgccc acccacaatc aagggtgtat tatccatgcc atagaacatg ctgcggatgt
      421 cttgatacaa tggttcacca tcggcaccgg gttcttttgt gcggtcgaga actgctatac
      481 gttttacgga tgagggaaga accttgaaga aatattttga gctgaatgga cgataaagat
      541 gaacgcaaat gaggccgatc ttttcgccct gtttcaattt ataatcgatg acttccttaa
      601 tagtttcagt aaccgaaccc atggcgatga taatattttc ggcatcgagg gcgccataat
      661 aatcgaaggg atgatgttcg cggccagtaa ttctggtcat ttcattcata taatgttcta
      721 ccagatcggg cacggcatca taaaaacgat tggaggcttc acgagcctga aagaaaatat
      781 cgggattttg tgctgtaccg cgggtaacgg gatgttcagg attaagagcc cggctacgga
      841 aggcagctaa ggcttgtttg tcgagaagac ttgcaacttc ggcttcaccg aatacttcta
      901 ttttttgaat ttcgtgactg gtacgaaaac catcgaagaa atgaaggaaa ggaatacgtg
      961 ccttgacagt cgttaaatgt gcaacaggag caatatccat gatttcctga acgcttccac
     1021 tggccaatag agcaaaaccc gtttggcgtg tggccatcac atcactgtga tctccaaata
     1081 tagagagtgc atgagtggca atggtgcgtg cgcttacgtg gaataccgct ggaagtaatt
     1141 cacctgccat tttgtacatg ttcggaatc
//
LOCUS       DAORPD010000692         1169 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_25579_length_1169_cov_1.628366, whole genome shotgun sequence.
ACCESSION   DAORPD010000692 DAORPD010000000
VERSION     DAORPD010000692.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25579_length_1169_cov_1.628366"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            66..>1169
                     /locus_tag="PK976_07875"
     CDS             66..>1169
                     /locus_tag="PK976_07875"
                     /inference="COORDINATES: protein motif:HMM:NF017504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HRC67844.1"
                     /translation="MAANEIIKPDYLFEVSWEVCNKIGGIFTVLATKAGTIKKELDDN
                     YIVIGPDVWMETHTNPYFEEDHLLYRAWRMQATSEGLKVRLGRFKGKKGAVAILVDFT
                     SLFPQKDKILAHLWEKFGLDSITGGWDYIEPLLFGYAAGKVIESFYEYEVFPQEKVVA
                     HCHEWMSGGTILYLKENLPQIATVFTTHATVLGRSISGHNWPLYRNLEFYNPETIARE
                     LGVVAKYSLEKTAALNTDILATVSNISAAEARKFFGRDVDYITPNGFDVSLIPDSDFE
                     TQRIEARKILIHTARTLLGQDIRDDAFMVVTSGRYEFRNKGLDAFIDALGYLDKNYRG
                     DRQIIAWLMVPFTNKGPNPVLKNRLEEKEPIVPT"
BASE COUNT          347 a          228 c          256 g          338 t
ORIGIN      
        1 ttgcactaag ggagtatctt tgttgtcgat tcatttatca aacattccta actaaaagaa
       61 ttgcgatggc tgctaatgaa ataataaaac ccgattatct ttttgaggtc agttgggaag
      121 tatgtaacaa gattggtgga atttttactg ttttagccac caaggctggt acaataaaaa
      181 aggaactgga tgataactat attgtaattg gacccgacgt atggatggaa acccatacga
      241 atccgtattt cgaggaagac caccttttgt acagagcgtg gcgtatgcag gcaacttctg
      301 aaggattgaa agtaagactt ggtagattta agggaaagaa gggtgccgtg gctattttgg
      361 tagatttcac ttccttgttc cctcaaaaag acaaaatatt ggctcattta tgggaaaaat
      421 tcggactcga ttccattaca ggaggttggg attacataga acctctgttg tttggatatg
      481 cggctgggaa agtcatcgag agtttttacg aatatgaagt attccctcag gagaaagtag
      541 tcgctcattg tcatgaatgg atgagtggag gtacaattct ttatttaaaa gaaaatcttc
      601 cacaaattgc tacagttttt accactcatg ccactgtttt aggcagatcc atttcggggc
      661 ataactggcc tttataccgc aatctggagt tctataatcc tgaaaccata gcacgtgaat
      721 taggtgttgt tgcaaaatat tcactcgaaa aaaccgctgc tttgaatacc gatattcttg
      781 caaccgtaag caatatttcg gcagccgaag cgcgtaagtt tttcggccga gatgtagatt
      841 atattacacc caatggtttc gatgtttcat tgattccaga ttctgatttc gagacccagc
      901 gcatcgaagc tcgcaaaatt ctcatccaca cggccagaac acttttaggc caggacataa
      961 gggatgatgc tttcatggtg gttacgagtg gccgctacga atttcgtaac aaaggcctcg
     1021 atgctttcat cgatgccttg ggttatctcg ataaaaacta tcgaggcgac cgacaaatca
     1081 ttgcatggct tatggtaccc tttaccaata aaggacccaa tcctgtcttg aaaaaccgtc
     1141 tggaagaaaa agaacctatc gtccctacc
//
LOCUS       DAORPD010000693         1168 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_25605_length_1168_cov_1.751123, whole genome shotgun sequence.
ACCESSION   DAORPD010000693 DAORPD010000000
VERSION     DAORPD010000693.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1168
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25605_length_1168_cov_1.751123"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..489)
                     /gene="rpsH"
                     /locus_tag="PK976_07880"
     CDS             complement(91..489)
                     /gene="rpsH"
                     /locus_tag="PK976_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HRC67845.1"
                     /translation="MTTDPIADYLTRLRNAIHANHRIVEIPSSTIKKSMTQILFEKGY
                     IINYKFEDDKFPSTIKIALKYHPVTKACAIKELKRISRPGRRIYTSADKMPRVLNGLG
                     IAIISTSQGLMTDKEARKLNIGGEVICYIS"
     gene            complement(521..790)
                     /gene="rpsN"
                     /locus_tag="PK976_07885"
     CDS             complement(521..790)
                     /gene="rpsN"
                     /locus_tag="PK976_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HRC67846.1"
                     /translation="MAKESMKAREVKRKMLNEKYAAKREALKKAGDYVALQKLPRNAS
                     PVRLHNRCKITGRPKGYMRDFGLSRINFREMASAGLIPGVKKASW"
     gene            complement(799..>1168)
                     /gene="rplE"
                     /locus_tag="PK976_07890"
     CDS             complement(799..>1168)
                     /gene="rplE"
                     /locus_tag="PK976_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009432221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HRC67847.1"
                     /translation="IAGQRAIATKSKKDISNFKLRRNMPVGVKVTLRGARMYEFLDRL
                     ISIAIPRIRDFHGISDKGFDGRGNFSFGISEQIIFPEINIDKVNKIMGMDITFVTSAE
                     TDAEAYALLKAFGFPFKNEK"
BASE COUNT          310 a          231 c          243 g          384 t
ORIGIN      
        1 aatgaaatga tcggggctga gcgaaacttt cactcctgaa ggtatttcaa taggtttttt
       61 accaattctc gacattttaa ctggttttaa ttagctaata taacaaatca cttcaccgcc
      121 aatatttaat ttgcgggctt ccttatctgt catcaaacct tgtgaggtag agataattgc
      181 tatgccaagg ccattgagaa cacgaggcat tttatcggca gaggtataaa ttcgacgtcc
      241 aggacggctt atgcgtttga gttcctttat agcgcaagcc tttgttacag gatggtattt
      301 caaggcgatt ttaatggtag aagggaattt atcgtcttcg aacttatagt tgattatata
      361 tcctttttcg aagagtattt gtgtcatgct tttctttatc gttgaagaag ggatttcaac
      421 aatcctatgg ttggcgtgga ttgcgtttct caatcgggtc agataatctg caatgggatc
      481 ggtagtcata tatctattta tttttattac aaattgatgg ttaccagctt gcctttttca
      541 cacctgggat aagaccagcc gaagccattt ctcgaaagtt gatgcgcgaa aggccaaaat
      601 cgcgcatata gcctttaggg cgtccggtaa tcttacaacg gttgtgcagc cttacgggag
      661 aagcattgcg aggaagtttc tgaagggcta cataatcgcc ggccttcttc aaagcttctc
      721 gcttggcagc atacttttca ttcaacattt ttcgttttac ttctcgcgct ttcattgatt
      781 cttttgccat aagcctgatt atttttcatt tttaaatggg aatccaaatg cttttaacaa
      841 tgcataagct tcggcatcgg tttcggcaga agtaacaaac gtaatatcca tgcccataat
      901 cttattgact ttatcaatat ttatttcggg aaagatgatt tgttcagaaa tgccgaagct
      961 aaaatttcct ctcccatcga agcctttatc gctaatgccg tgaaaatcac gaatacgtgg
     1021 aatggcaatg gagatcaagc gatccaggaa ctcatacata cgagcgcctc taagggttac
     1081 tttcactcca acgggcatat tgcgacgcag cttgaagttg gagatatctt ttttcgattt
     1141 ggtggctata gctctttggc cagctatt
//
LOCUS       DAORPD010000694         1165 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_25727_length_1165_cov_2.057658, whole genome shotgun sequence.
ACCESSION   DAORPD010000694 DAORPD010000000
VERSION     DAORPD010000694.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25727_length_1165_cov_2.057658"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1165
                     /locus_tag="PK976_07895"
     CDS             <1..>1165
                     /locus_tag="PK976_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HRC67848.1"
                     /translation="KWNKNSECNVSDYEVYRSTDNGSSFQLAGTTKKTDTIFNDYITS
                     KLSVTKLTYGVKALNDMGQPSAMSELVETYLKNLSDEELLDMVQEATFRYFWEYGHPV
                     SGMARERLGSGEIVTTGGTGFGIMGILCGIHRNFITRQQGLERILKIVDFLSQTDRFH
                     GAWPHWLNGTSGKVIPFSNYDNGGDLVETAFLIQGLLTARSFFDGEYLEDTLRQKITQ
                     LWESVEWDWYTRNGSNVLYWHWSPNYQWIMNMPIRGFNETMIVYILAIASPTHPVAAS
                     LYHMGWASGNYLNGKSFYGYPLAVGWDYGGPLFFTHYSFLGFDPRSKKDAYANYFNNN
                     RNIALIHHAYCIDNPLNHLGYGDSCWGLTASDDPDGYLAHQPHTNNDNGTISPT"
BASE COUNT          346 a          238 c          250 g          331 t
ORIGIN      
        1 caaatggaac aagaattctg aatgcaatgt cagtgactat gaggtttatc gttcgaccga
       61 caacggctca agttttcaat tggcaggaac caccaagaaa accgacacaa tatttaatga
      121 ttatattact tccaaacttt cggttaccaa attaacttat ggtgtaaaag ccttgaatga
      181 tatgggacaa ccttcggcaa tgtcggagct ggtcgaaact tatctcaaaa acctgagcga
      241 tgaggaactt ctcgatatgg ttcaggaagc caccttcaga tatttctggg aatatggaca
      301 cccggtgtca ggtatggcac gcgaaaggct tggatcgggt gaaatcgtta ctactggtgg
      361 aacaggtttt ggaattatgg gcatattatg cggtattcat agaaatttta taaccagaca
      421 gcaaggattg gaaagaattt tgaaaatcgt tgattttctc tcccaaaccg acagatttca
      481 tggtgcatgg cctcactggc tgaatggaac cagcgggaag gttatcccgt tttcaaatta
      541 cgataatgga ggtgatctgg tggaaactgc cttcttgatt caaggacttt taaccgctcg
      601 tagcttcttt gatggagaat atctcgaaga taccttacgt caaaaaatta ctcaattatg
      661 ggaaagcgtc gaatgggatt ggtacacccg aaatggaagt aatgtacttt actggcactg
      721 gtcgccaaat taccaatgga taatgaatat gcccatacgg ggcttcaacg aaactatgat
      781 tgtttatatt ctggccatcg cctcccctac tcatccagtg gcagcttcat tatatcacat
      841 gggatgggca agtggaaatt acctcaatgg aaaatcattc tatggatatc ccctcgcagt
      901 aggttgggac tatggaggac cgcttttctt cactcattat tcctttttag gtttcgaccc
      961 aagaagtaaa aaagatgcct atgccaatta tttcaataat aatcgcaata tagccttaat
     1021 ccaccatgct tattgtatcg ataaccccct gaatcatttg ggttatggag attcatgctg
     1081 gggattgaca gctagcgatg atcctgacgg atatttagcc catcagcctc atactaataa
     1141 cgacaatggc actatttctc caacc
//
LOCUS       DAORPD010000695         1162 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_25864_length_1162_cov_2.216802, whole genome shotgun sequence.
ACCESSION   DAORPD010000695 DAORPD010000000
VERSION     DAORPD010000695.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25864_length_1162_cov_2.216802"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1162)
                     /locus_tag="PK976_07900"
     CDS             complement(<1..>1162)
                     /locus_tag="PK976_07900"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRC67849.1"
                     /translation="DALFNYAKLSYELASDPYNESISALKKYLTEFPESHRTDEANQY
                     LINLALISKNYKQALEAIENIKFKDIWIKTAQQRILYYLGIESFNENKYQEAIHYLQE
                     AAGMDFDKTIRLQALYWTAEAIYRTGDYVSALKAYHKFLLSAGAVDIPEYTSVHYNIG
                     YAQFKLSQYDEASVSFRKFLSTYQGNDKAMINDAYLRIGDCFFIGRNYTEAIKYYQKA
                     YNNGSTEADYALYQIALSQGVQKQFQEKIATLKNLISKYPRSPYNPAAYYELALTSLL
                     INKNNDALGYLDQIINKYPKSNYVSKSMLKKGMIYYNDNQNEKALVELKAVVNKYPGT
                     SDAQEALVAISNIYVEMNRVDEYLSYLKTVPNAELLMSGQDSLIYVAAENQY"
BASE COUNT          326 a          214 c          244 g          378 t
ORIGIN      
        1 tgatattgat tttcggccgc tacataaatc agggagtctt gtcccgacat taaaagttca
       61 gcattgggca cagttttcag atacgacagg tattcatcta cccgattcat ttctacataa
      121 atattactga tggccaccag tgcttcctga gcatcagacg taccgggata tttattgact
      181 actgccttta attctaccag ggctttttca ttctgattgt cgttataata aatcatccct
      241 ttctttagca tggatttgct cacataattg ctcttaggat atttattgat aatctggtcg
      301 aggtaaccta aagcatcatt gttcttgttg ataagaagag aggtgagggc taattcgtag
      361 taagccgctg gattgtatgg agagcgcgga tatttggaga ttaaattttt cagcgtagca
      421 attttttcct gaaactgttt ttgaacacct tgcgaaagag caatctgata cagggcataa
      481 tcggcttcag tacttccatt gttataagct ttctgataat atttaatggc ttcggtataa
      541 tttcgaccaa taaaaaagca atccccaatt ctcaggtaag catcattgat catggcttta
      601 tcgttgcctt gataagtaga aaggaatttt ctaaacgaga ccgaagcctc atcgtattga
      661 ctcaatttaa actgtgcata cccgatgtta tagtgcacgg aggtgtattc aggaatatcg
      721 acggcgcccg cgcttagtaa aaatttatga taagccttga gagccgaaac ataatcgcct
      781 gttcgataaa tggcttcggc agtccaatat agagcctgaa gccgtatggt tttgtcgaaa
      841 tccataccag cagcttcttg cagataatga atggcttcct gatacttatt ttcattgaac
      901 gattctatgc caagataata aaggatgcgt tgctgagcag ttttaatcca tatgtcttta
      961 aacttaatgt tttcgatggc ttcgagcgct tgcttgtaat ttttgctgat gagagcaagg
     1021 tttatgagat attgattggc ctcatcggtg cgatgcgatt cggggaactc ggtcagatat
     1081 ttttttaatg ccgagatgga ttcattatat ggatcggaag ccaattcata agaaagttta
     1141 gcataattga acagagcatc tt
//
LOCUS       DAORPD010000696         1161 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_25904_length_1161_cov_2.397830, whole genome shotgun sequence.
ACCESSION   DAORPD010000696 DAORPD010000000
VERSION     DAORPD010000696.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25904_length_1161_cov_2.397830"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /gene="pyrH"
                     /locus_tag="PK976_07905"
     CDS             <1..379
                     /gene="pyrH"
                     /locus_tag="PK976_07905"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859761.1"
                     /note="Catalyzes the phosphorylation of UMP to UDP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HRC67850.1"
                     /translation="LQAIAYLEAGKVVIISGGTGNPYFTTDTASALRGIEVKADILLK
                     GTRVDGVYTADPEKSPNAVKYSRLTFQEAYSKGLKILDLTAFSLCMENNLPIYVFNMD
                     VKGNLIRLIKGEHIGTLISNNFQ"
     gene            385..>1161
                     /locus_tag="PK976_07910"
     CDS             385..>1161
                     /locus_tag="PK976_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HRC67851.1"
                     /translation="MNQFPPSIPKGTRDFVPVEMHRRDFIFSTIRQVFQLYGFQPIET
                     PAMENLQTLLGKYGEEGDKLLFRILNSGNYLQNVPIECLHSGEPQKILPFIAEKGLRY
                     DLTVPFARYVVQHHDEITFPFKRYQIQPVWRADRPQKGRYREFYQCDADVIGSKSLLN
                     EGEFINIIDEVFSRLNIKVSIRLNNRKILAGIAEYIGHGKQLIDITTSIDKLDKIGLD
                     EVDNELSKRGISNKAIEKLHPLLFLIGENEEKLNELQSYFT"
BASE COUNT          362 a          271 c          217 g          311 t
ORIGIN      
        1 tcttcaagcc attgcttacc tcgaagcagg taaagttgta attatcagcg gaggtactgg
       61 aaacccatac tttactaccg atacggcttc agcacttaga ggaatcgaag tcaaagccga
      121 tatcctcctg aaaggcacac gtgtggatgg agtttatacc gccgatcccg aaaaaagccc
      181 taatgcggta aaatattcaa ggcttacctt tcaggaagca tatagcaaag gcctcaaaat
      241 actcgatctt accgccttca gtctttgcat ggaaaacaac ctccctatct atgtcttcaa
      301 catggatgtg aaaggcaacc tgattcgact catcaagggc gaacatatcg gaacccttat
      361 ttccaataat tttcagtaaa ttccatgaat caatttcctc catccatacc taaagggact
      421 cgtgattttg tccctgtaga aatgcacagg cgcgatttca tctttagtac cattcgtcaa
      481 gtttttcagc tttacggttt tcagcccatc gaaactccag ctatggaaaa cctccaaacc
      541 cttctgggta aatatggaga agaaggcgat aaactactgt tccgaatcct caattcagga
      601 aattatcttc agaacgtacc tatcgagtgt ttgcattcgg gcgaacctca gaaaattctc
      661 cccttcatcg ccgaaaaagg attgcgttat gacctcaccg taccttttgc acgttatgtg
      721 gttcagcacc acgatgaaat taccttccca tttaaaagat atcaaattca acccgtttgg
      781 cgagccgaca ggcctcaaaa aggaagatac agagaattct accaatgcga tgccgatgtg
      841 attggaagca aatctttgct caacgaaggc gaattcatca acatcatcga tgaagtcttt
      901 tcacgattaa atatcaaggt ttccatacgg ctgaacaacc gtaaaatttt ggctggcatt
      961 gctgaatata taggccatgg taagcaactg atcgacatta ccacttccat agataagctc
     1021 gacaaaattg ggcttgatga agttgacaac gaactttcaa aacgtggcat atccaacaag
     1081 gctattgaaa agctccatcc tctgcttttt ctcataggag aaaatgagga aaaactgaat
     1141 gaacttcaat catattttac t
//
LOCUS       DAORPD010000697         1160 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_25941_length_1160_cov_2.532127, whole genome shotgun sequence.
ACCESSION   DAORPD010000697 DAORPD010000000
VERSION     DAORPD010000697.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25941_length_1160_cov_2.532127"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /gene="rmuC"
                     /locus_tag="PK976_07915"
     CDS             <1..375
                     /gene="rmuC"
                     /locus_tag="PK976_07915"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HRC67852.1"
                     /translation="EPDLYSYALEKHIILVSPTTLLATLRTVEMIWRQEKQQKNIKEI
                     IRLASNMYDKLALLVKKLEELGRNIRKSSDLYDDVMRTLRTGKGNLMQQAFKMQELGT
                     PVTKKLSPPNETLSFDEEETEN"
     gene            353..>1160
                     /locus_tag="PK976_07920"
     CDS             353..>1160
                     /locus_tag="PK976_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67853.1"
                     /translation="MKKKRKIKVSVISSILLLILQGSCTSSKLLQNNNFASDMLAGSM
                     KALTGIQVYGANPDSIHLYLEIDPSLLKPQALPDGLEFLSLSLRWMIFSSFSEKSIID
                     SSTFRTGPLSPHQSEKLIFEHSLKAPRGKSYYLCYKPSSDRYRNWNIMWIDRTNEQSH
                     NHFLITLSDSTLIFRSEELFDKPLYIYHSVPTDNTNLFLRRINLCSCTPSPPFALKVG
                     ACKIRFDSIKTIRIHHGKSEEFLLENPGLYFIQIDTNLIGAGSFLVLNPEF"
BASE COUNT          367 a          256 c          200 g          337 t
ORIGIN      
        1 gagcctgatc tctattctta tgctctcgaa aaacatatta ttctggtaag ccccaccacc
       61 ctcttagcca cgctaagaac agtggaaatg atctggcgtc aagaaaaaca acaaaaaaat
      121 ataaaagaaa ttatccgctt agcctcgaac atgtacgata aacttgcctt attggtaaag
      181 aaactcgaag aattgggcag aaacattcgg aaatcttccg acttatacga tgatgtgatg
      241 cgaactttga gaactgggaa gggaaattta atgcaacagg cttttaaaat gcaggaatta
      301 ggaaccccag ttacgaaaaa attaagtcca cctaacgaaa cgctctcgtt cgatgaagaa
      361 gaaacggaaa attaaggtaa gtgtaatttc atccatcttg ttattgattt tgcaaggatc
      421 gtgcacatct tccaaactct tacaaaacaa caactttgct tcggatatgc tggccgggtc
      481 tatgaaggca ttaacaggta tacaggtcta tggtgccaat ccagatagta tacaccttta
      541 tctcgaaatc gatccatctt tgttgaaacc gcaagcctta cctgatggct tagaatttct
      601 ttcgctcagc cttcgatgga tgattttttc atctttctcg gaaaaatcca ttatcgattc
      661 ttccactttt cgaacaggcc ctctgagtcc ccatcaaagt gaaaaactca tattcgaaca
      721 ttccttgaaa gcgcctcgag gaaaatccta ttatttatgc tataaacctt cttccgatag
      781 gtacagaaat tggaatatta tgtggattga ccgaactaac gagcaaagtc ataatcattt
      841 tctcatcacc ctatccgata gcactttgat attccgttcg gaagaattat tcgataaacc
      901 cttgtatatc taccattccg ttcctacaga taatacaaat ttattcctca ggaggataaa
      961 tttatgttca tgcactccct ctccaccctt tgcactcaaa gtcggcgctt gcaagattcg
     1021 gttcgactcg ataaaaacca taagaattca tcacggcaaa tcggaagaat tcttacttga
     1081 aaatccaggg ttatatttta tccagataga caccaatctc attggagccg gctctttcct
     1141 tgtattaaat cctgagtttc
//
LOCUS       DAORPD010000698         1157 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_26042_length_1157_cov_2.557169, whole genome shotgun sequence.
ACCESSION   DAORPD010000698 DAORPD010000000
VERSION     DAORPD010000698.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26042_length_1157_cov_2.557169"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..1120
                     /locus_tag="PK976_07925"
     CDS             89..1120
                     /locus_tag="PK976_07925"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRC67854.1"
                     /translation="MFRFENPEYFYFLLIIPISVALFVVGRIIREKMLARFGEKSLLS
                     RLMPDYSNVRPILKLILWNIAMIILTIAMANPQIGTKLVKAKQKGIDMMICLDVSNSM
                     LAEDVKPNRLENAKLAIRKLVEQLQGDRIGIIVFAGKAYIQLPITSDYAAAEMFINNI
                     NTRLIPTQGTALAQAISMAKASLQSETRSKAIILITDGEDHEGDVLTEAEKAAAEGIR
                     IYTIGIGTPEGSPIPIVNEKGEITGYKKDAQGNTIITRLDEITLQKVASTGQGIYIRA
                     QNTNQALRKIFEEINKLEKTEFDTQLFSEYESWYPYFLWIAFVLLVLEMLIPARKSHW
                     AKRINLFGQ"
BASE COUNT          380 a          206 c          233 g          338 t
ORIGIN      
        1 gctataatcc tcctcatcat tgaattcatc ttgtcgcata cttttttaag ggtttatcct
       61 taaagttatt tttaaaattt tgaataatat gttcagattt gaaaatccag aatattttta
      121 ttttttattg ataattccaa tttcggtggc tctttttgtg gtaggaagaa tcatacgcga
      181 aaagatgcta gcgcggtttg gagagaaaag ccttttgagc cggttgatgc ctgattattc
      241 gaatgtgcgt cctattttaa aacttatttt atggaatata gccatgatta tattgaccat
      301 agctatggcc aaccctcaaa taggaacaaa acttgtaaaa gccaaacaga agggcatcga
      361 catgatgatt tgcttggatg tttcgaacag tatgcttgcc gaagacgtaa aacctaatcg
      421 gctggagaat gccaaactgg ccatacgcaa attagtcgaa caattgcagg gcgataggat
      481 aggtattatt gtctttgcag gcaaggctta tattcagttg cccatcacta gtgattatgc
      541 tgccgcagaa atgtttatca acaacatcaa cacacgactg attcctactc aaggaacggc
      601 tcttgctcaa gccattagca tggcaaaggc ttcattacaa tcagaaacac gtagcaaagc
      661 cattatattg attactgacg gcgaagacca tgagggagat gttttgacag aagccgaaaa
      721 agcagctgct gaaggcattc gtatttacac cattggaatt ggtacacctg aaggttcacc
      781 tatcccaatc gtcaatgaaa aaggagaaat aactgggtac aagaaagatg ctcagggcaa
      841 tacgattatt acacggctcg atgaaattac acttcaaaaa gtagcatcga ccggacaagg
      901 catttatatc agggcacaaa ataccaatca agccttaaga aaaattttcg aggaaattaa
      961 caagctcgaa aaaaccgaat tcgatactca gttattttcg gaatatgaaa gctggtatcc
     1021 ttacttttta tggattgcgt ttgtgctttt agttctggaa atgttgattc cagcacgaaa
     1081 aagccattgg gcaaaacgga taaatctttt tggacaataa actatttata gtgatggaaa
     1141 aaagatattt atgtttg
//
LOCUS       DAORPD010000699         1157 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_26059_length_1157_cov_1.616152, whole genome shotgun sequence.
ACCESSION   DAORPD010000699 DAORPD010000000
VERSION     DAORPD010000699.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26059_length_1157_cov_1.616152"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..>1157
                     /locus_tag="PK976_07930"
     CDS             24..>1157
                     /locus_tag="PK976_07930"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRC67855.1"
                     /translation="MKRILIILLLTCCALRLPAQILISFKTDSTCKTDTIIVPLKVQE
                     LRQCASFRIEFSFQDDIVHYDSLYFRNPALAGLTAFLNGNNRLVLEWTSASALPFGTG
                     NIAKIQFTALQTGTSLLSFDAAQTYFKDEQGNPLTYLYNDGILKVHPRGIQYNLTQLM
                     MGCSKEDKGRYGISIHSGTPPYQIDWGDGFLMPGIDTVVLGVSPGQHKITIVDGNGCI
                     YRDNYFVKVLKAPPIHFTFDPDTVYLQKPSVQFYSNIDSLLNVGIDVYNFQWNFGEPD
                     SSRSVEKNPLHTFTSAWQFFQQQIEEYRVRLYAINNDNCDTSIAKFLPLRRPKVEVPN
                     VFTPNADGINDVLIIKVDGKTETEQPSLLRYYERMELVILNRWG"
BASE COUNT          320 a          238 c          230 g          369 t
ORIGIN      
        1 atcaagtggt aaaaatttct cagatgaaac gtattctcat tattctactt ctaacatgtt
       61 gtgctttacg gcttcctgcc caaattttaa tttctttcaa aaccgatagt acttgtaaaa
      121 ccgacactat tattgttcca ttaaaggttc aggaattaag gcaatgtgcc tcctttcgga
      181 ttgaattttc atttcaggat gatattgttc attatgacag tctttatttt cgtaaccctg
      241 cactggccgg tcttaccgct ttcttaaacg gaaataatcg tctggttctg gagtggacat
      301 cggcctccgc tttacccttt ggaacgggca atattgcgaa gattcaattt acagccctgc
      361 aaactggtac ttctttactt tcttttgatg ctgctcaaac ctacttcaaa gatgagcaag
      421 gcaatcctct aacttattta tataacgatg gtatcctgaa ggttcatcca cggggcatac
      481 aatataattt aacccagttg atgatgggct gcagcaagga ggataaggga cgttatggca
      541 tttccattca ttcggggact cctccctatc aaatcgattg gggcgatggc ttcttgatgc
      601 ctggaataga tactgtggtt ctgggcgtta gcccaggtca gcataaaata accattgtag
      661 atgggaatgg ttgtatttat cgcgacaatt acttcgtaaa agtcctaaaa gcacctccta
      721 ttcattttac tttcgatccc gatacggtct atctgcagaa accttctgtg caattctatt
      781 ctaatatcga ttcgcttctt aatgttggga tagatgtata taattttcaa tggaattttg
      841 gtgaacccga ttcgagccgc tctgtcgaaa aaaatccctt acataccttt accagcgctt
      901 ggcaattttt tcagcaacaa atcgaagaat accgtgttcg actctatgct attaataatg
      961 ataattgcga tacaagtatt gcgaaattct tgccgctgcg acgccctaaa gtggaagtgc
     1021 ctaatgtttt tactcccaat gccgacggaa tcaatgatgt tttaattatt aaagtggatg
     1081 ggaaaacgga aaccgagcaa ccttcattgc tgcgatatta tgaacgaatg gaactggtga
     1141 tcctgaacag atgggga
//
LOCUS       DAORPD010000700         1156 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_26080_length_1156_cov_2.924614, whole genome shotgun sequence.
ACCESSION   DAORPD010000700 DAORPD010000000
VERSION     DAORPD010000700.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26080_length_1156_cov_2.924614"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1156)
                     /locus_tag="PK976_07935"
     CDS             complement(<1..>1156)
                     /locus_tag="PK976_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HRC67856.1"
                     /translation="TGHSSTSISAALGMAIAAKLRGDARLQHIAVIGDGAMTAGMAFE
                     AMNHAGSTRANILIILNDNGIAIDRNIGALKEYLAQFTASQTYNKFRNRIWNLLGGDS
                     KYGTNTRHIVKQVGNALKSSLLNRSNLFEAMGFRYFGPVDGHDVVKLSHLLHDLKNIP
                     GPKLLHVITKKGKGLEKAELDPTTYHSPGTFNKETGELDKSSCEEKLPPKYQNVFGKT
                     IIELARNNPRIVGITPAMSTGCSLNLMMEVMPERTFDVGIAEQHAVTFSAGLAAAGMI
                     PFCNIYSSFMQRAFDQAIHDVCLQKLHVVFCLDRAGIVGEDGATHHGAFDLAYFRTIP
                     NLIVSAPLNEKELRNMMYTAQLNGIGPFSIRYPRGRGVLNEWRTPFEAIPI"
BASE COUNT          299 a          250 c          267 g          340 t
ORIGIN      
        1 caatgggtat ggcttcgaaa ggggttctcc attcgtttaa tactccgcgg cctcgcggat
       61 atctgataga aaaagggcct attccattga gttgagctgt gtacatcatg ttgcgaagtt
      121 ctttttcgtt taagggtgca gaaacgatga gattgggaat tgttctaaaa taggccagat
      181 cgaaggctcc atgatgcgtt gctccgtctt cacctactat tccagctcta tcaagacaaa
      241 aaaccacatg gagtttttgc agacaaacat catgaattgc ctgatcgaaa gctctttgca
      301 taaacgaaga atagatattg cagaaaggaa tcataccagc agcagcaagc cctgccgaaa
      361 acgtaacggc atgttgttcg gctatgccga catcgaaagt tctttcgggc attacttcca
      421 tcatcaagtt gagagagcaa cccgtagaca tggcaggcgt aatccctaca attcggggat
      481 tgttgcgggc aagttcgatg atagtctttc cgaatacatt ttgatattta gggggtagtt
      541 tttcctcgca gctcgattta tcgagctcgc cggtttcctt gttgaatgtc cctggggaat
      601 gataagtagt aggatctaat tctgcctttt cgagtccttt gccctttttt gtgattacat
      661 gcaatagttt aggtcctgga atatttttta aatcatggag taaatgtgat aattttacca
      721 cgtcgtgtcc atcgaccgga ccaaaatatc tgaatcccat agcctcgaaa agattgctgc
      781 ggttcaaaag agatgatttc agagcattac ctacctgttt gacgatgtgg cgagtattcg
      841 tgccatattt actatctccg cccaataggt tccaaatcct gtttctaaac ttattataag
      901 tctgtgaggc agtgaattga gcgagatatt ccttcagtgc tccgatgttt ctatcaatcg
      961 caatgccatt gtcgttgagg atgatcagaa tgtttgctcg ggtgcttccg gcgtgattca
     1021 tagcttcaaa ggccattcct gcagtcatgg caccatcgcc gatcacggct atatgctgaa
     1081 ggcgtgcgtc acctcgaagt tttgcagcaa tagccattcc aagagcagcc gagatactgg
     1141 tcgaagaatg ccctgt
//
LOCUS       DAORPD010000701         1154 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_26179_length_1154_cov_2.131028, whole genome shotgun sequence.
ACCESSION   DAORPD010000701 DAORPD010000000
VERSION     DAORPD010000701.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26179_length_1154_cov_2.131028"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..110)
                     /locus_tag="PK976_07940"
     CDS             complement(<1..110)
                     /locus_tag="PK976_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HRC67857.1"
                     /translation="MNQRIRIKLKSYDYNLVDKSAEKIVKTVKQTGAVVSG"
     gene            complement(136..>1154)
                     /gene="fusA"
                     /locus_tag="PK976_07945"
     CDS             complement(136..>1154)
                     /gene="fusA"
                     /locus_tag="PK976_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130823.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRC67858.1"
                     /translation="NKQNPLEKIEAGDIGAAAGFKQIRTGDTLADEKHPILLESITFP
                     EPVISMAVEPHTQDELEKMMNALQKISEEDPTFFVGIDEETGQTLISGMGELHLDIIL
                     DRLHREYNVECNHGKPQVAYKEALTRNVRHHEIYKKQTGGRGRFADIEFEIGPADIGV
                     KGLQFINEIHGQNLPKEYIPAIEKGFRSAMSNGPLLGFPIESMKVRLIDGQAHPVDSD
                     ALSFEIAAISGFREAIRKAECILLEPIMKLEVVTPDEYVGDITSDLNRRRGQLLKIDA
                     RISYQIVDALVPLSEMFGYVTVLRSISSGRATSSMEFSHYATCPPEIQEQVILIIKGY
                     IIRQ"
BASE COUNT          296 a          246 c          228 g          384 t
ORIGIN      
        1 ccgctcacta ctgcacctgt ttgttttact gttttcacaa tcttttcggc tgatttatct
       61 accagattgt aatcgtacga tttcaactta attctaatcc tttggttcac gtttatagag
      121 aattaaaaat cattttcatt gtcttataat atagcctttt attattaata tgacctgttc
      181 ctgaatttca gggggacaag ttgcataatg cgaaaattcc atgctcgacg tagctcttcc
      241 actcgaaatc gacctcaata cagtaacata accgaacatt tcggaaagag gtacaagtgc
      301 atccacaatc tgataactaa ttcttgcatc aattttcaat aattgtcctc ttcttcggtt
      361 taaatcgctg gtaatatcgc caacgtattc atcaggagtt acgacttcga gtttcataat
      421 aggttcgaga agaatacatt ctgctttgcg aattgcttcc ctaaatccac tgatggcagc
      481 aatttcgaac gaaagggcgt cgctatccac tggatgagct tggccatcta tcaaacgaac
      541 tttcatgctt tcgatgggaa atcccaataa aggtccattc gacatggcag aacgaaatcc
      601 cttttcgatg gcaggaatat attctttcgg gaggttttgc ccatggattt cgtttatgaa
      661 ttgtaaacct ttgacgccta tgtcggctgg tccaatttca aattcaatat cggcaaaacg
      721 gcctcggcca ccggtttgtt ttttatagat ttcgtgatgg cgaacattac gagtaagtgc
      781 ttctttgtat gccacctgag gtttgccatg attgcattcc acattatact cgcggtgtag
      841 cctatcgaga ataatatcga gatgcagttc acccattcca ctaatgagtg tttggccggt
      901 ttcttcatcg atacctacga aaaatgtggg gtcttcttca ctgatttttt gcagggcatt
      961 catcatcttt tcgagctcgt cctgtgtatg tggttctact gccatactga tgacaggttc
     1021 aggaaaagta atggattcga gcagtatagg atgtttttca tcggcaaggg tgtcgcctgt
     1081 ccgaatttgt ttaaaacctg ctgcggctcc aatatcgcca gcttcaattt tctcgagagg
     1141 attttgttta ttgg
//
LOCUS       DAORPD010000702         1151 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_26279_length_1151_cov_2.245438, whole genome shotgun sequence.
ACCESSION   DAORPD010000702 DAORPD010000000
VERSION     DAORPD010000702.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26279_length_1151_cov_2.245438"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /locus_tag="PK976_07950"
     CDS             <1..885
                     /locus_tag="PK976_07950"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006952199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isomerizing glutamine--fructose-6-phosphate
                     transaminase"
                     /protein_id="HRC67859.1"
                     /translation="EDLAHIPVEVEYSSEFRYRNPVIYPDDIVIAISQSGETADTIAA
                     IQMAKQKGAFLYGICNVVGSSIARYVDAGSYTHAGPEIGVASTKAFTAQVTVLALMAL
                     ALGQKKGTISNQKVREVLTEMETIPDKVRQILDKAEMIRDFSKVFTYARNFLYLGRGY
                     NFPVALEGALKLKEISYIHAEGYPAAEMKHGPIALIDQEMPVVVVATNRGTYEKVISN
                     IEEVKSRKGRIISVITEGDTRVRKLSDYYIEIPETEEMLVPILATIPLQLMAYYIAVL
                     RGCNVDQPRNLAKSVTVE"
     gene            921..1046
                     /locus_tag="PK976_07955"
     CDS             921..1046
                     /locus_tag="PK976_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67860.1"
                     /translation="MLLRNRKGELYQTGQFLSNQTLLSMVVLAQFDLLTFGGNIK"
BASE COUNT          360 a          259 c          223 g          309 t
ORIGIN      
        1 gaagatttag cccatattcc tgtcgaagtt gaatattcct cagaatttcg ttatcgcaat
       61 cccgtcattt atcctgacga cattgtgatt gccatcagtc aatcgggtga aactgccgat
      121 accattgccg ccattcaaat ggcgaaacaa aaaggagcat ttctctatgg aatatgcaat
      181 gttgtgggta gttcgattgc ccgctatgta gatgcaggct catataccca tgcgggtccc
      241 gaaattgggg ttgcctcaac caaggctttt accgcacagg taaccgtgct tgcacttatg
      301 gcactggcac tgggtcaaaa gaaagggacc atctccaacc aaaaggttag ggaagtactt
      361 accgaaatgg aaactattcc cgataaggta cgacaaatac tcgataaggc tgaaatgatt
      421 agagattttt cgaaagtatt tacttatgca cgtaatttcc tttatcttgg cagaggatac
      481 aatttcccgg ttgccctcga aggagcctta aaacttaaag aaatttccta tattcatgcc
      541 gaaggttatc ctgcagcaga aatgaagcac ggccccattg cactgatcga ccaggaaatg
      601 ccagtggtag tcgttgctac aaatagaggt acctacgaaa aagtgatctc caacatcgaa
      661 gaagttaaat cacgaaaagg acgcattatc tcggtcatca ccgaaggcga tacaagagta
      721 agaaagcttt ccgattatta tatcgaaata cccgaaaccg aagaaatgct cgttcccata
      781 ctggcaacca ttccattgca actgatggct tattatatcg ccgttcttcg tggatgcaat
      841 gtcgatcaac cacgaaatct ggctaaatcg gttacagtgg aataatcccc cggactactt
      901 acaaaagaaa aaccttctcg atgcttcttc gcaatcgaaa aggagaatta tatcaaacag
      961 gccaattcct atccaatcaa accttacttt cgatggtggt attggctcaa ttcgatttgc
     1021 ttacttttgg gggaaacata aaataaatca ctattaaccg acaaaattct aaaaagctcc
     1081 taaacatttt ttatatacca cttattcagt tagtcctgac ttcgacaatg cgtcacccta
     1141 aaaatttttc c
//
LOCUS       DAORPD010000703         1146 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_26503_length_1146_cov_2.961503, whole genome shotgun sequence.
ACCESSION   DAORPD010000703 DAORPD010000000
VERSION     DAORPD010000703.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26503_length_1146_cov_2.961503"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..>1146)
                     /locus_tag="PK976_07960"
     CDS             complement(64..>1146)
                     /locus_tag="PK976_07960"
                     /inference="COORDINATES: protein motif:HMM:NF021554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2279 domain-containing protein"
                     /protein_id="HRC67861.1"
                     /translation="TDWIFIRLCVNRKFSCKFGILSLNNSRVPKPFIAIFTVTLVYFP
                     VFLFPLALEESLYDSQDSIIAADTLKTLRWWESAIEYSPQRMRYIVGGGSLMYGVTLV
                     GLNYLWYEDYPRSSFHFFDDSGEWLQVDKAGHVVTPYLEAYYLQYIFRWAGMKPKPAA
                     IYAGITAFMLQNTIEVFDGFSAKWGASWSDVAANFVGSALMTSQELLWNEQRMRLKII
                     HHFPDYPQGELSQRARDLYGKTLPERLFKDYNALTVWLSMNPSSFGLSLGQPEWLNLA
                     MGYGAANMYGGYNNIWTDENGILHDRSDIERYRRYFISPDIDFSRIKTKSRFLRIFFE
                     TLNIIKVPAPTLEINSSGQIIFHLLY"
BASE COUNT          368 a          251 c          214 g          313 t
ORIGIN      
        1 ttgtccctat tgcacaatta tcgaaatgga aacaagttac agagaaattc catcaatatt
       61 actttaatat agaagatgaa aaattatctg tccacttgaa ttgatttcga gagttggtgc
      121 aggaactttg atgatattca aggtttcgaa aaaaatcctt aaaaaccttg atttggtttt
      181 tattcggctg aaatcgatat cgggagaaat aaaatacctt cggtagcgct ctatatcgct
      241 tctatcgtgc aatattccat tctcatccgt ccatatattg ttataaccgc catacatatt
      301 ggcagctcca tagcccatgg ctaaattcag ccattcaggt tgccctaagg atagcccaaa
      361 cgaagaggga ttcatgctca gccaaacggt caatgcatta taatctttga acaagcgttc
      421 tggaagtgtt tttccataaa gatcacgggc tctttgagag agttcaccct gtgggtaatc
      481 gggaaaatga tggatgattt tgagccgcat gcgttgctca ttccataaca attcctgtga
      541 agtcatcaaa gctgagccca caaaatttgc tgcgacatcg ctccacgatg ccccccattt
      601 tgccgaaaaa ccatcgaata cttcaatggt attctgaagc ataaatgcgg taattcctgc
      661 atatattgca gcaggcttcg gtttcattcc cgcccagcgg aaaatatact gaagataata
      721 tgcctctaag taaggtgtaa ccacatgacc cgccttatct acctgaagcc attccccaga
      781 atcatcgaaa aaatgaaacg atgaccgagg ataatcctca taccataaat aatttaaacc
      841 gacaagcgtc accccataca tcaaagaccc tcctccaaca atatatctca tccgttgagg
      901 gctatattcg atagctgact cccaccatcg caatgtttta agcgtgtcgg ctgcgataat
      961 ggaatcctga gaatcatata aagattcttc caaggcgagt gggaataaaa aaacaggaaa
     1021 atagaccaag gttacggtga atatagcaat gaatggcttt ggcactctgc tgttatttag
     1081 agataatatg ccaaatttac atgaaaactt tcgattgaca cacagacgaa taaaaatcca
     1141 gtccgt
//
LOCUS       DAORPD010000704         1146 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_26504_length_1146_cov_2.784601, whole genome shotgun sequence.
ACCESSION   DAORPD010000704 DAORPD010000000
VERSION     DAORPD010000704.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26504_length_1146_cov_2.784601"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            165..>1146
                     /locus_tag="PK976_07965"
     CDS             165..>1146
                     /locus_tag="PK976_07965"
                     /inference="COORDINATES: protein motif:HMM:NF014754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HRC67862.1"
                     /translation="MENRKGDSIEKQLNYIDGRALYYGFLAGANKIFENQHQLNKINV
                     FPVADADTGTNFASTMRSIIDMVEPNRDVKTTVDSLANAALIGARGNSGIIFAQFLYG
                     FSTGIGPTESLSVANFAMALKESVKYAYQAVTNPVEGTILTVIREWADFIYQIKDSIN
                     DFSILLENSIDKARISLEQTKIKLQVLARANVVDAGAKAFVLFLEGIIDLLKKGIKIL
                     LTSHQASKVEELSPIFHDEITFRYCTEALISGNGIDQQKIKKEMSFMGDSLVVAGSSS
                     KTRIHIHTDNPSGVFKRLMNHGQIIYQKVDDMAFQNTVASSQRSKVAILTD"
BASE COUNT          374 a          231 c          225 g          316 t
ORIGIN      
        1 aatcgttcca tacaaaatat tgtatctcaa gtttggaatt tattttcttg ggaatgcact
       61 ttctcttatt accgaagaac gcaaagatcc tcagcctaaa gaattttatc cataatgagg
      121 ttctatatag agtgaaggaa taaaatttta atcaagacta atttatggaa aataggaagg
      181 gcgactcgat cgaaaaacag ttgaattata tcgatgggag ggcattatat tacggttttt
      241 tggcgggtgc caataaaata tttgaaaatc aacatcagct caataaaatc aatgttttcc
      301 ctgtagccga tgccgacaca ggcacaaatt ttgcctctac catgcgctct atcatcgata
      361 tggtagaacc caaccgcgac gtaaaaacca ctgtagattc gttagccaat gccgcattaa
      421 tcggagcaag agggaattca gggatcatat tcgcacagtt tctctatggc ttcagtacag
      481 gcatagggcc caccgagtcc ctgagcgttg caaactttgc catggccttg aaagaatcgg
      541 taaagtatgc atatcaggcc gtcaccaatc ctgttgaagg aactatttta accgtcatca
      601 gagaatgggc tgattttatc tatcaaatta aagattccat caatgatttc agcatattgt
      661 tagaaaattc gatcgacaag gccagaatat ctctggaaca aacaaaaata aaattgcagg
      721 tactggcaag agcaaacgtc gtagatgccg gtgccaaggc ctttgtattg tttttagaag
      781 gaattatcga tcttttaaaa aaaggaatca aaattctact tacttctcat caagccagta
      841 aggttgaaga attatcgcct atctttcacg acgaaataac cttcagatac tgtaccgaag
      901 ctctaattag tggtaatggc atcgatcagc aaaaaatcaa gaaggagatg tcttttatgg
      961 gagattcgtt agttgttgcc ggctcttcga gtaaaacccg tatccacatc cataccgaca
     1021 acccctccgg agtctttaag cgcctgatga atcatggaca aattatttat caaaaagtcg
     1081 acgacatggc gtttcaaaat accgtcgctt cgagccaaag gtcaaaagta gccatactta
     1141 ccgatt
//
LOCUS       DAORPD010000705         1145 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_26559_length_1145_cov_1.985321, whole genome shotgun sequence.
ACCESSION   DAORPD010000705 DAORPD010000000
VERSION     DAORPD010000705.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26559_length_1145_cov_1.985321"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(298..429)
                     /locus_tag="PK976_07970"
     CDS             complement(298..429)
                     /locus_tag="PK976_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67863.1"
                     /translation="MNKLPGNILLGVKAGIFFSGSQLQINELKPTPLLPQLGYPVIL"
BASE COUNT          368 a          221 c          204 g          352 t
ORIGIN      
        1 ggtatctgca gataattttg taaatattta taagattcga gatggatcac ttttataaca
       61 ggatcggcta caaaactttt tcgtgtgagg ataccaatca agaacttcag aaagacaagt
      121 aagaatgggg aggagatcac tgattcttta tagacttctt tcgtcaatgc tatcatgttc
      181 tatggtttcg atgattcaaa gtgagccagg aatgcagatt aggaaacgga gtgccagatc
      241 caaagtgaca ttggcaaaat tcagaacagc tttgagatca tttgcatgac ctgttagtta
      301 caaaataaca ggataaccca gctgaggaag aagtggagtg ggtttgagtt cgttgatctg
      361 caattggctt ccgctgaaga aaattcctgc ttttactcct aaaagaatat ttcccggcaa
      421 cttattcaaa aacagaaaaa gtcgaaactg tgctgagcaa gtaaattttt tgtcttttct
      481 ttgactatgg gcgaaaaatt aaaatattca tcacaaattc tgtaaataga aattaatttt
      541 cttcagtatt gggtaggaat atgaattttc ccactttctt attcaaattt ctggtcggtt
      601 agtactgatt tgaaatatgg caaacatgat gaactactta gtaaacatga tcgtccccag
      661 ttgcttactt gccttcgtat ttttaaaacc atctacatag aagcgaatta tttcgctaaa
      721 cataacctgt tttctaactt ctaccatcga ttcgagtaag gtctattagt tcgatattga
      781 ttttgagggc aaaggataga acacattcgc ttgccatgta attatcttaa tgaatcatta
      841 tgaatttttt acgaattttt tgataagtac tcagaaaacg aggccttatt tgattttaaa
      901 gaatgaatcc ccataaataa agcgtcctaa gaggagtgat tcaatataag attcaaaatt
      961 tcagtaaggg aaggcgaata aattttttac cagcttcccg aagcgcctcc accaccgaag
     1021 ctacctcctc caaatccccc gaagctgctc cctccgccac caaaccatcc acttcccgat
     1081 gaaaaattgt cccatttccc cttatgacct cctcggccaa acatgttcat caaaacaatc
     1141 atcgg
//
LOCUS       DAORPD010000706         1143 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_26636_length_1143_cov_1.927390, whole genome shotgun sequence.
ACCESSION   DAORPD010000706 DAORPD010000000
VERSION     DAORPD010000706.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26636_length_1143_cov_1.927390"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1129)
                     /locus_tag="PK976_07975"
     CDS             complement(<1..1129)
                     /locus_tag="PK976_07975"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HRC67864.1"
                     /translation="MEHAANIALPVSTLSSGYEWLYAHELSHMWFGDKITCSSAEDMW
                     LNEGWAVFNESLYREGIYGYPAYRSNMNSKLANVLQYCHIKDNGYRALYGIPNEYTYG
                     ETVYQKGGVVVHTLRNYLGDSLFFPAISNFLQDYAFQPVSSFQLRDYLTQYTGIDMTP
                     FFDGWVFSPGFPCFVIDSCQMVPSGQNFLTTVFVHQKLKGAPEYYHNNRLFISFIDSL
                     WNTHDFMMEFSGEFGSQTFVLPFKPTLCLADYYDRIADATTDASLRIHSSGDYDFPNT
                     FFRLSITSLADSAFFRVTHNWAAPDSLKTPLPGLTLSDYRYWRIEGIYHVPFQAKGRF
                     FYSRPSHLDDSLLQNLNDSLVILYRKNASEEWQGIAFTRTGT"
BASE COUNT          342 a          226 c          285 g          290 t
ORIGIN      
        1 gagtgcctgt gcgagtaaag gctattccct gccattcttc ggaggcattt tttcgataaa
       61 ggatgacgag agaatcgttg aggttttgca gcagagaatc gtcgaggtgg gaaggacggg
      121 agtagaagaa tcgtcccttg gcttgaaaag gtacgtggta aataccttcg atgcgccaat
      181 aacgataatc ggaaagagtc aatcccggca gaggagtctt aaggctatcg ggagctgccc
      241 agttgtgtgt tactctgaaa aatgccgaat cggcaaggga ggttatgcta agcctgaaaa
      301 aagtatttgg aaaatcgtaa tctccggaac tatgtattcg taaactagca tcggtggttg
      361 catcggcaat tctatcgtaa taatcggcaa gacatagtgt gggtttaaaa ggtaggacaa
      421 acgtctgact gccaaattct cccgaaaatt ccatcataaa atcatgcgta ttccacaaag
      481 aatcaataaa gctgatgaac aaacggttgt tatgataata ttcaggcgct cctttcagct
      541 tttgatgaac aaaaaccgta gtaagaaaat tttgtcctga aggaaccatc tgacaggagt
      601 cgatcacaaa gcagggaaat cctggggaga acacccatcc atcaaaaaaa ggtgtcatgt
      661 ctatcccggt atattgggta agataatctc tcaattggaa agaagaaacg ggttggaagg
      721 cataatcctg tagaaaattg ctaatggcag ggaagaaaag gctatcgccg agatagtttc
      781 tgagcgtatg aacaacaacg ccaccttttt ggtaaacggt ttctccgtag gtatattcat
      841 tgggaattcc ataaagagct ctatagccgt tgtccttgat atggcaatat tgcagtacgt
      901 ttgcaagttt ggagttcatg ttgctgcgat aggccggata gccatatatt ccttcacgat
      961 acagagattc gttgaagact gcccatcctt cgttgagcca catatcctcg gccgatgagc
     1021 aggtaatctt gtcgccgaac cacatgtgcg agagttcatg cgcataaagc cattcatagc
     1081 ccgacgaaag ggtgctcacc ggaagggcaa tattagcagc gtgttccata gctccttgta
     1141 tgg
//
LOCUS       DAORPD010000707         1142 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_26677_length_1142_cov_2.304508, whole genome shotgun sequence.
ACCESSION   DAORPD010000707 DAORPD010000000
VERSION     DAORPD010000707.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26677_length_1142_cov_2.304508"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1142)
                     /locus_tag="PK976_07980"
     CDS             complement(<1..>1142)
                     /locus_tag="PK976_07980"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRC67865.1"
                     /translation="LITDPSSPYSGQRWSTRTEFPKDVISTGWNDGLDISWIGYGEGV
                     WFVVMSGNTGIEDQRWITNGDFPSGEIKEAIKAGESVLQLVYGNDRWAAVFGKTEHYG
                     QQIFGLYSDFPAKTIKEYWSKGYYITSISPCKGKWGLVMTKDAPFENQFYVSDKKFPE
                     AEVKSKIAEGYEITDIGYGNGIWLVVMSVPIETRIPGEEEEFTYDPDEAEPSQRAAPQ
                     KEIEVNPKAIRYFQTGMEYFNRKEYQKAISYFEDALKITPEFPSALNGIGAAWSWLGD
                     MDKALKYYEDSYRLDHSNPVVFSNLVYTLNDMGKTEEIYQTALECPSATLKEVEDAMP
                     LYHAGKALLEKGKAKRALEYFKLASKLEPDESLFKDAIAEAEANPE"
BASE COUNT          292 a          300 c          212 g          338 t
ORIGIN      
        1 cctcgggatt ggcttctgcc tcggctatgg cgtctttgaa aagggattca tcgggctcga
       61 gcttcgatgc cagcttgaaa tattccaatg ccctcttggc cttgcctttt tcgagaagcg
      121 cttttccggc atgatacaac ggcatggcgt cttccacttc cttcaaggta gccgatgggc
      181 attccaaggc agtctgataa atttcttccg tcttccccat atcattcaac gtatagacta
      241 aattgctgaa aacaaccgga ttggagtgat cgagtcgata cgaatcttca taatatttca
      301 aggctttatc catatctcca agccacgacc aggcagctcc tatgccattt agagccgaag
      361 gaaattctgg agtaatcttc agggcgtctt caaagtacga aatagccttt tgatattcct
      421 ttcgattgaa atactccatg cccgtttgga aatatcgaat ggccttcgga ttgacttcta
      481 tctccttttg gggagcagcc ctctgagatg gttcagcttc atcgggatca taagtaaatt
      541 cttcctcttc tccagggatt cgagtttcta taggtaccga cataactacc agccaaatcc
      601 cattcccata accgatatct gtaatttcat aaccctcggc aattttcgat ttgacttcgg
      661 cctcggggaa ttttttatcc gatacataaa attgattctc gaaaggagca tccttagtca
      721 ttaccaaacc ccatttccct ttgcatggac ttatcgaagt aatataatat cccttcgacc
      781 agtactcctt tatggttttt gcaggaaaat cactgtaaag gccgaaaatt tgctgtccat
      841 aatgttcggt cttcccaaac actgcggccc atctatcgtt tccgtaaacc agctgtaaaa
      901 ctgactctcc cgccttaata gcttctttaa tctcgccaga cgggaaatcc ccattggtta
      961 tccatctttg gtcttcaata ccggtgttac ctgacatcac cacaaaccaa actccttctc
     1021 catagccaat ccagctaata tcgagaccat cattccatcc agtagaaata acatccttcg
     1081 gaaattcggt tcttgtgctc catcgctgcc ccgagtaagg actcgaagga tcggtaatca
     1141 ac
//
LOCUS       DAORPD010000708         1140 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_26750_length_1140_cov_2.131797, whole genome shotgun sequence.
ACCESSION   DAORPD010000708 DAORPD010000000
VERSION     DAORPD010000708.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26750_length_1140_cov_2.131797"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="PK976_07985"
     CDS             complement(<1..507)
                     /locus_tag="PK976_07985"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRC67866.1"
                     /translation="MLAKNILNSLILPLEIHQTGYDALNRMDDYHIGQLPVISNGEYF
                     NLIREDEIFEYNLPDTPLKEFTFSSRIPPALSNQHLLEVLEIMAEYNLEIIAVVNEKN
                     MYQGCISLYDILKALYHLDMIQNPGGILVLELNLHDYSLTQIAQILEANDAKVLGLYT
                     ISPPDSTKV"
     gene            complement(519..>1140)
                     /gene="rsgA"
                     /locus_tag="PK976_07990"
     CDS             complement(519..>1140)
                     /gene="rsgA"
                     /locus_tag="PK976_07990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HRC67867.1"
                     /translation="TGFIDRFLLTAEAYEIPVYLVFNKMDLYNDDDLELLDTFAKYYA
                     SCGYGIIFTSVVMHQGLDELKEVLHNRTTLLAGHSGVGKSALIHAIEPSLNIKIGEIS
                     AYHKKGKHTTTFAQMHPLSHGGFIVDTPGIKEFGIIDFKKEEVALYFPEMRKLLPQCQ
                     FYNCTHDHEPGCAVKQAVKDGEIASWRYHNYLSVIHGEEIENAFSS"
BASE COUNT          322 a          211 c          269 g          338 t
ORIGIN      
        1 cactttggtg ctatcgggag gtgaaatggt ataaagtccg aggacttttg catcgttggc
       61 ttccaagatt tgagctatct gtgtcaggct ataatcgtgc agattaagtt cgagcacaag
      121 aattcctcct gggttttgaa tcatgtcgag atgatagagt gcttttagga tgtcgtacag
      181 actgatgcag ccttgataca tatttttttc gttgaccacg gcaataattt cgagattata
      241 ttcggccatg atctccaaga cttcgaggag atgctgattg gataatgcag gagggatacg
      301 cgaagaaaag gtaaattctt tgagtggtgt atcgggcagg ttgtattcaa aaatttcatc
      361 ttcacgaata agattgaaat attctccgtt agaaattacg ggaagctgcc ctatgtgata
      421 atcatccatt cgattgagag catcgtatcc ggtttgatgg atttcgagag ggagaatgag
      481 tgaattgagt atattcttgg caagcataaa caagggtttt aagatgaaaa ggcgttttcg
      541 atttcttcgc catgaatcac gctgagataa ttatggtagc gccatgaggc aatctcacca
      601 tctttgacgg cttgttttac ggcacagccg ggctcatgat cgtgagtaca gttgtaaaat
      661 tggcattgcg gcaatagttt acgcatctca ggaaaataca aagccacttc ttctttcttg
      721 aaatcgatga tgccgaattc tttaatgcct ggggtgtcga caataaagcc accatgagaa
      781 agaggatgca tctgggcaaa ggttgtagtg tgtttccctt ttttatgata ggccgatatt
      841 tcgccaatct ttatgttcaa cgagggttcg atggcgtgaa taagtgccga tttccctaca
      901 ccagaatgcc ctgccagtaa agtggttcta ttgtgaagca cttccttgag ttcgtccaat
      961 ccttgatgca taacgacaga ggtaaaaatg atcccataac cgcaactggc ataatatttt
     1021 gcaaaagtat ccaatagctc gagatcatca tcgttgtata ggtccatttt gttaaaaacc
     1081 agataaactg gaatctcata ggcttctgcc gtcaaaagga aacggtcgat aaatccagtg
//
LOCUS       DAORPD010000709         1139 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_26795_length_1139_cov_2.166052, whole genome shotgun sequence.
ACCESSION   DAORPD010000709 DAORPD010000000
VERSION     DAORPD010000709.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26795_length_1139_cov_2.166052"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="PK976_07995"
     CDS             complement(<1..308)
                     /locus_tag="PK976_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67868.1"
                     /translation="MKKPIGLFMIILLIFSCTNRISKQEFSLEDLQKRTFRYFWVSAD
                     PVTNLVPDRWPTPSFSSIAAQGFGYPAWIIGIENGWVTREDVALKVKNSLKFLYNLPQ
                     G"
     gene            447..>1139
                     /gene="tsaD"
                     /locus_tag="PK976_08000"
     CDS             447..>1139
                     /gene="tsaD"
                     /locus_tag="PK976_08000"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HRC67869.1"
                     /translation="MSIYILGIESSCDDTSAAVLRDDLLLSNVIANQEIHYKFGGVVP
                     ELASRAHQQSIIPVVDQAIWQAKLTPQQIQLVAFTQGPGLLGSLLVGVSFAKAFALAL
                     GIPMTGCDHMQAHILSHFIRKPGCKHRVPSFPFLCLTVSGGHTQIVKVDDYDKMKIMG
                     RTIDDAAGETFDKAAKIMGLPYPGGPVIDRHARKGNPMAFSFAKPLIAGLDFSFSGLK
                     TSFLYFLRDRLKE"
BASE COUNT          300 a          224 c          262 g          353 t
ORIGIN      
        1 ccttggggaa gattatagag aaatttcaat gagtttttta ctttgagggc tacgtcttca
       61 cgcgttaccc agccattttc tatcccaata atccatgccg gataaccaaa tccctgggct
      121 gctatgctcg aaaaggatgg ggtgggccaa cgatcgggta caagattggt taccggatcg
      181 gcacttaccc aaaaatacct gaaggttctt ttttgaaggt cctcgagtga gaattcttgt
      241 ttcgagattc tattcgtaca cgagaaaatc agcagaataa tcataaaaag accgataggt
      301 tttttcatat acttatgctt aggaagtgca aaataataac aactgcattc gtaaagcaat
      361 gttttatggt agaactatta aattttgtta ggtagatatg ttctaatttt gtaggtttaa
      421 tgataaaaac agattaatga tgagcgatga gtatttatat attggggatt gaatcttcgt
      481 gtgatgatac ttcggctgct gtgctccgtg acgatttatt gctaagtaat gtaattgcca
      541 atcaggagat ccattacaag tttggtggtg tggtacccga attggcttcg agggcacatc
      601 agcagagcat tattcctgtt gtggatcagg ctatttggca ggctaaactt actccacagc
      661 aaattcagct tgtagcattt acgcaagggc caggtttgtt gggttcactt ctggtggggg
      721 tttcttttgc caaagccttt gcgttggccc ttggtattcc tatgacgggt tgcgaccaca
      781 tgcaggcaca tatcctatct catttcatcc ggaaacctgg ctgcaaacat cgagtcccat
      841 cttttccctt tctatgtctt accgtttcgg gaggtcatac gcaaatcgta aaagtcgacg
      901 attacgataa gatgaaaata atgggacgta cgatcgacga tgccgcaggt gaaactttcg
      961 ataaggccgc taaaatcatg ggattgcctt atccgggggg gcctgtaatc gatcgacatg
     1021 ctcgtaaggg aaatcctatg gctttttctt ttgctaaacc cctgattgct ggtctcgatt
     1081 ttagctttag cggcttgaaa acttccttcc tgtatttttt acgcgaccgt ctgaaagaa
//
LOCUS       DAORPD010000710         1138 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_26853_length_1138_cov_1.438596, whole genome shotgun sequence.
ACCESSION   DAORPD010000710 DAORPD010000000
VERSION     DAORPD010000710.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26853_length_1138_cov_1.438596"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="PK976_08005"
     CDS             <1..547
                     /locus_tag="PK976_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67870.1"
                     /translation="MEISKMYFDLSELDSALKFASEAALAPGPKELNFRWELYMLLGE
                     ILQAQNNLDQAKEHILLALKLRQEKGWKEITALSSLTAELQVDANDGRSTAQIQKNLM
                     GFWKGIVHVDKKQISGKIKNLVGEGRSGFIIGDDGKDYYFKVKSFQHRQSEVKPGMRV
                     RFFIQSSNDQRCDSAVDIELQ"
     gene            655..>1138
                     /locus_tag="PK976_08010"
     CDS             655..>1138
                     /locus_tag="PK976_08010"
                     /inference="COORDINATES: protein motif:HMM:NF012534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HRC67871.1"
                     /translation="MTQKIRGIVDWYDEEKGYGVIRGENGNDYFIHHSNLSLSLKKGF
                     HVIFQTRPRSHNKQGFEAYDVSLESYDDFSKEKKNDSRILPINGKKFTEDKSKINYAN
                     KLINEKQTDVSSTKVNTIQPQSFKSLIFTKLHRLIHIENGYITDRVKGTYHYQKTVSR
                     L"
BASE COUNT          415 a          181 c          231 g          311 t
ORIGIN      
        1 gatggaaatt tcaaagatgt atttcgattt atctgagttg gattcagctt tgaaatttgc
       61 atccgaggct gcgcttgcgc caggaccaaa agagctcaat tttcgctggg agctatatat
      121 gcttttagga gaaatcctcc aagcacaaaa taacttagat caagccaaag aacatatttt
      181 attagcattg aaattaaggc aggaaaaagg atggaaggag ataacagccc tttcttcact
      241 aactgccgag ttgcaggtgg atgcgaacga cgggcgttca actgctcaga ttcagaaaaa
      301 tctgatgggc ttttggaaag gaatagttca tgttgacaaa aagcaaattt ctggaaagat
      361 taagaattta gtcggagaag ggcggtcagg gttcataatt ggggatgatg gaaaagatta
      421 ttattttaag gtcaaatcat ttcagcatcg acaatctgag gtaaaacctg gtatgagagt
      481 taggttcttt attcaatcta gtaatgatca gcgatgtgat tcagctgttg acattgaact
      541 tcaatgaaaa attcagaatt gaacaaaatg ccgagaaaaa acagataaaa tggtattatg
      601 tcataattcc aatatcgtaa aatacacttc attcactgga agatgaagtc gaaaatgact
      661 caaaaaattc ggggaattgt tgattggtac gacgaagaaa aaggttatgg ggtaatccga
      721 ggggaaaacg gaaatgatta cttcatccat cactcaaatt tgtcattatc tcttaagaag
      781 ggattccatg taatatttca aacaagacct cgttcccata acaaacaagg gtttgaggca
      841 tatgatgtgt cactcgagtc atatgatgat ttttctaaag aaaaaaagaa cgattcgagg
      901 atattgccaa taaatggaaa aaaatttacc gaggataaat ccaagatcaa ttatgcgaat
      961 aagcttatca atgaaaaaca aaccgatgtt tctagcacaa aagtcaacac catccaaccg
     1021 caaagtttca aatccttaat ttttacaaaa ctacaccgat taattcatat agagaatggt
     1081 tatataactg atcgagttaa agggacgtat cactatcaaa aaacagtttc acggcttt
//
LOCUS       DAORPD010000711         1137 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_26877_length_1137_cov_1.715342, whole genome shotgun sequence.
ACCESSION   DAORPD010000711 DAORPD010000000
VERSION     DAORPD010000711.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26877_length_1137_cov_1.715342"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="PK976_08015"
     CDS             complement(<1..265)
                     /locus_tag="PK976_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HRC67872.1"
                     /translation="MNKNLQIAKYVVADYLTALLAWSLFFFYRKYYVDPYFLEHYTVV
                     FDDPKFFYGIIGIPFFWLILYAISGQYRKIYRKSRLKELGHTLI"
     gene            complement(269..>1137)
                     /locus_tag="PK976_08020"
     CDS             complement(269..>1137)
                     /locus_tag="PK976_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67873.1"
                     /translation="IAFLSCEDFEGEQTIPAYLKIDSIALKIVSPSQGSASSKITDAW
                     VYIDEQLIGAFELPATIPLLYTGKHRLTIIPGIRMDGMVQLRAYYPLYNQIRREIFLS
                     PDSVTIIKGNLINGKNTIYTTYKDLVQFSWSENFEDENLALDTTKKSNVNFMLTPEDD
                     PATFEGLHSGKIILTLTDTIFEAMTSEAISLPRNGSPVFLEMNYRCTNTFTFGIIALG
                     SDQYIQSPILDLNPSASWNKIYINLAPNVSSSDDALKFKLFWGAMLDIGQEQTEIYFD
                     NMKLVYLNNQSK"
BASE COUNT          356 a          198 c          238 g          345 t
ORIGIN      
        1 tgatcaaagt atgacccaat tccttcaacc tcgatttacg ataaatttta cgatattggc
       61 ctgatatagc ataaagaatt agccagaaaa agggaattcc aataatgccg taaaagaatt
      121 tgggatcatc gaaaaccacg gtataatgtt cgagaaaata aggatcgaca taatatttgc
      181 ggtaaaagaa aaacaagctc catgctagta gtgcagtaag ataatcagca acaacatatt
      241 tagcaatttg taaattttta ttcataaact acttcgactg attatttaaa taaaccaatt
      301 tcatattatc gaaatatatc tcggtttgct cctgcccaat atcgagcatg gctccccaga
      361 agagtttaaa cttaagggca tcatcagagc tactaacgtt tggagccagg tttatataaa
      421 ttttattcca acttgcagat gggttcagat cgaggattgg agattgaata tattgatcgc
      481 ttcccagtgc aatgattccg aaagtaaatg tgtttgtgca acgatagttc atttcgagga
      541 acactggtga gccattacgg ggcagactta tggcttcgga agtcatggct tcgaaaatgg
      601 tatcggtcaa tgtaagaata attttccctg aatgtaagcc ttcgaatgtg gctggatcat
      661 cttcgggagt gagcataaaa ttgacattgc ttttttttgt agtatcaagg gcaaggtttt
      721 catcttcgaa attttcactc caggaaaatt gaaccaaatc cttgtatgtg gtatagattg
      781 tatttttccc gttgattaaa tttcctttta tgatggtaac cgaatcaggg gaaagaaaga
      841 tttcgcggcg tatttgattg tagagggggt aataagctcg cagttgcacc attccgtcca
      901 ttctgatgcc aggaataata gtaagacggt gtttgccggt ataaagcaag ggaatagttg
      961 caggcagttc gaaagcacca attagctgtt catcgatata aacccatgca tctgtaattt
     1021 tgctcgaagc acttccctgt gaaggtgata cgatttttaa agctatgctg tcaattttta
     1081 aataagccgg aatggtttgt tcgccttcaa aatcctcgca tgacaagaac gcgattg
//
LOCUS       DAORPD010000712         1135 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_26965_length_1135_cov_1.339815, whole genome shotgun sequence.
ACCESSION   DAORPD010000712 DAORPD010000000
VERSION     DAORPD010000712.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26965_length_1135_cov_1.339815"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="PK976_08025"
     CDS             <1..218
                     /locus_tag="PK976_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67874.1"
                     /translation="LLSKQVTGKSGGDFGMLDASGMGNYFDTRFCKLRWKIAICIPIA
                     CGIFHGYGSEKHGGEKTALLKITFCYF"
     gene            complement(274..558)
                     /locus_tag="PK976_08030"
     CDS             complement(274..558)
                     /locus_tag="PK976_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67875.1"
                     /translation="MSAVQDLRHCRHTLYNLGNGWDRLLYLFGFLKRIRGETMLGKLI
                     IRGAYNLSIAVDKAPKIMGKLATILGVAVAEVAILLFFLLLSFRLEALLK"
     gene            594..1049
                     /locus_tag="PK976_08035"
     CDS             594..1049
                     /locus_tag="PK976_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67876.1"
                     /translation="MALVDDLLLIHADATFYTSYGATIDAVNRALHDLLHSDDPPAWD
                     RLLDQFGERKAFLDDDPDGAARAVQMLADFGGHQIIHGHTPISYVLRCFPEEVIEPLV
                     YANGLCVNVDGGMYLDGPGFIYRPADGWVLHGPLPSRDTVASAEDDLSA"
BASE COUNT          268 a          301 c          286 g          280 t
ORIGIN      
        1 aacttctatc caagcaagtg accggtaaat cgggtgggga cttcggaatg ctcgatgcga
       61 gtggcatggg caactacttc gatacccgct tctgcaagct cagatggaag atagcgatct
      121 gcatcccaat cgcctgcggc atttttcatg gctacggctc cgaaaaacat ggcggcgaaa
      181 aaaccgccct gctaaaaatc actttttgct acttctaaag tgaattatag cagagcggtt
      241 ttcgctttca agagtttttt cggaccgcaa tgattatttc aggagggcct ccaaacggaa
      301 gctaagaagc aggaagaaca gcagtatagc gacctctgct acggcaactc cgagaatcgt
      361 tgctaacttc cccattattt tcggggcctt atcaaccgct atgcttagat tataagcacc
      421 tcggattatc aattttccta gcatagtttc tcctctgatt ctcttcaaaa aaccgaacag
      481 ataaagtaag cgatcccacc cgttaccgag gttatagaga gtatggcggc aatgacgaag
      541 atcttgcacg gctgacaccc aagcatgctg aatggcttag tagcttgcca gcaatggcgc
      601 tggttgacga cctgctgctt attcatgccg atgcaacctt ctacacgagc tatggcgcca
      661 caatcgatgc tgtgaaccgt gcacttcacg atttactcca tagcgatgac ccacccgcct
      721 gggatcggct gctcgaccag tttggtgaac gtaaggcatt cctcgatgat gaccccgatg
      781 gtgcggcgcg cgccgtccag atgctggccg atttcggcgg gcatcagatt atccacggcc
      841 atacccccat cagctatgtg ctgcgctgct ttcctgagga agtgattgag ccgctggtat
      901 atgcgaatgg cctgtgcgtg aatgtcgatg gtggcatgta cctcgacggc ccgggcttca
      961 tctatcgccc cgccgatggc tgggtattgc acggcccgct ccccagccgc gataccgtag
     1021 ccagcgccga agatgatctg agcgcttgac gtcgtttggt atactgtaaa gcagcctaac
     1081 ggaaaatagt gttaggactt tcgcttacgc aagcaaattc tttctgttct tggtg
//
LOCUS       DAORPD010000713         1134 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_26999_length_1134_cov_1.879518, whole genome shotgun sequence.
ACCESSION   DAORPD010000713 DAORPD010000000
VERSION     DAORPD010000713.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26999_length_1134_cov_1.879518"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1101
                     /gene="nusB"
                     /locus_tag="PK976_08040"
     CDS             <1..1101
                     /gene="nusB"
                     /locus_tag="PK976_08040"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HRC67877.1"
                     /translation="IKVLQALYAYFQSDNHEIDLAEKQLFSSIQHIYDLAIYQISFLL
                     NLSRFAEERLEDAKLKFRPNEEDLNPNTRFIENAFLKKLESNQSLMKHIQKIKISWTE
                     YEDVTRRIYKTLRSSKEYQEYMAKSQISFREDKDFIVHIFNRLIVSDIGLQAIFEEKN
                     IFWTNDIHEETILLNKEILASENSDHLADREILLKIYRKEGNENEDIEVVCHEQSIYE
                     ANDYSIAAWLANRILNHLKEKLDESAPLPPLLKTSDITEENDREFLLGLFRKTILHDE
                     DYDEYISEKIINWDLERIAIMDAIILKMAITEILEFPYIPIKVSMNEYIELAKIYSTP
                     RSSQFINGMLDKIVDSLKEKNLIKKSGRGLLE"
BASE COUNT          389 a          203 c          220 g          322 t
ORIGIN      
        1 atcaaggttt tgcaagcttt atatgcctat ttccagtctg ataatcatga gatagacctt
       61 gctgagaagc aattattcag cagcatccag catatttacg atcttgctat ttatcaaatt
      121 tctttcttat tgaatttatc gagatttgct gaagaacgtc tggaagatgc caaattaaaa
      181 ttcagaccca atgaggaaga tttgaacccc aatactcgtt ttatcgaaaa tgctttctta
      241 aaaaaactcg agagcaatca gagcctgatg aagcatattc agaagataaa aatttcatgg
      301 acagagtatg aagatgttac cagaagaatt tacaaaacct tgagatcgtc gaaagaatat
      361 caggaatata tggcaaaatc tcaaatcagt tttcgtgagg ataaagattt cattgttcat
      421 atatttaatc ggttgattgt ttctgatatt ggattgcaag ccattttcga agaaaagaac
      481 attttctgga ccaatgatat ccacgaagag accattttac tcaacaaaga aattctggct
      541 tcggaaaatt ccgatcatct ggccgataga gaaatacttc tgaaaattta tcgtaaggaa
      601 gggaatgaaa acgaggacat tgaggtggta tgccatgaac aaagtattta cgaagccaat
      661 gattattcca tagctgcatg gcttgccaat cgcattctaa atcatttaaa agagaaattg
      721 gacgaatcgg ctccgcttcc acccttgctg aaaacttctg acattaccga ggaaaacgac
      781 agggagttcc tgttgggtct ttttcgaaaa acaattcttc atgatgaaga ttacgatgaa
      841 tatattagtg aaaagattat caactgggat ctcgaacgaa ttgccatcat ggatgccata
      901 atattgaaaa tggctattac cgaaatcctc gaattcccct atattccaat caaggtgagt
      961 atgaacgaat acattgaact ggcaaaaatt tacagtacac ctcgcagcag ccagtttatc
     1021 aatgggatgc tcgataagat cgtcgattcg ctgaaggaaa agaatctgat aaaaaaatca
     1081 ggccggggac ttttggagta aaccttcagg aggccttcta ctttcgaccg aaat
//
LOCUS       DAORPD010000714         1133 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_27026_length_1133_cov_2.174397, whole genome shotgun sequence.
ACCESSION   DAORPD010000714 DAORPD010000000
VERSION     DAORPD010000714.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27026_length_1133_cov_2.174397"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1133
                     /locus_tag="PK976_08045"
     CDS             <1..>1133
                     /locus_tag="PK976_08045"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRC67878.1"
                     /translation="EFSESLCMGLMALGFVQGDKIATITANRPEWNFVDMGLALGGFV
                     HVPIYPTLNVEEYYYILEHSDTRLVFVGDKSLYAKIKPIADALPQIQMVVTFNEVEGA
                     PHWMEIVETGRKMREKYDGPLKKIKNSIKPDDLVTIIYTSGTTGKPKGVMLSHWNIMS
                     NAIATSKINPLTHEDKVLSFLPLSHIYERMMNYDFQYLGIGIYYAENMGSIVANLNEI
                     QANEFNAVPRLLENVYDRIVAQGKDLNGIKKAIFFWALHLAERFEFNNANGWFYDLQL
                     KWADKLVFVKWREALGGHIKVIVSGGSSLQERLCRIFWGAGLPVVEGYGLTETSPVIA
                     VGNFKKNGVKFGTVGPILPGVQVDFAHDGEILCKGPNVMLGYY"
BASE COUNT          338 a          176 c          282 g          337 t
ORIGIN      
        1 aagaattttc ggagagtttg tgtatgggac taatggcact tggattcgtg caaggagata
       61 aaattgctac cattacagca aatcggcctg aatggaattt tgtggatatg ggccttgcac
      121 ttggcggatt tgtgcatgtt cccatttatc caactctcaa tgttgaagag tattattata
      181 ttctcgaaca ttcggataca aggctggttt ttgtaggcga taaatcactc tatgccaaga
      241 taaaacccat tgccgatgct ttgccacaaa ttcaaatggt ggttactttc aatgaagtgg
      301 aaggtgcgcc tcattggatg gaaattgtag aaacaggaag gaaaatgaga gaaaaatatg
      361 atgggccatt gaagaaaata aaaaacagta taaagcccga tgatttagtt actattattt
      421 acacatcggg tacgacgggc aagcccaaag gcgtcatgct gagccattgg aacattatgt
      481 cgaatgccat agccacttct aagatcaatc cattgacgca cgaggacaag gttttgagtt
      541 ttcttcctct ttcgcatatc tatgaaagga tgatgaatta tgattttcaa tatttgggca
      601 tagggattta ttatgcagag aatatgggca gtattgtggc gaatttaaat gaaattcagg
      661 ccaatgagtt caatgctgtt ccgcgattgc tcgagaatgt ttacgacagg attgtagctc
      721 aggggaaaga tttaaatggc atcaaaaagg caatattttt ttgggcattg catctggccg
      781 agcgttttga gtttaacaat gccaatggat ggttttatga tttgcagtta aaatgggctg
      841 ataaactggt atttgtgaaa tggagagaag ccttaggcgg ccatatcaag gtaatagttt
      901 caggaggttc gtcgttgcag gaaaggttat gtagaatttt ttggggtgcg ggtttgcccg
      961 tagtagaagg atatggattg acagaaactt caccggtaat tgctgttggc aattttaaaa
     1021 aaaacggagt taaatttggt accgttggcc ctattttgcc aggtgtacag gtagattttg
     1081 cccacgacgg agaaatcctg tgcaaaggac ctaacgtaat gttaggttat tac
//
LOCUS       DAORPD010000715         1129 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_27205_length_1129_cov_2.389199, whole genome shotgun sequence.
ACCESSION   DAORPD010000715 DAORPD010000000
VERSION     DAORPD010000715.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1129
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27205_length_1129_cov_2.389199"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="PK976_08050"
     CDS             <1..316
                     /locus_tag="PK976_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67879.1"
                     /translation="KEFGIITRLTQSEAAAEAQYHLSLITFNRNDLSNAEKMTFQLIN
                     NYPSADYWRVKGFILLSDIYAANGNIFQARQTLQSIIDNYKGEDLRKEAQNRLNNLPP
                     VQ"
     gene            366..>1129
                     /locus_tag="PK976_08055"
     CDS             366..>1129
                     /locus_tag="PK976_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67880.1"
                     /translation="MKLSTTIGVLLWLPIVEKSFGQHPQIEEVTIIGSFTPHISDANK
                     ILRNPMLRDTSIILKPMEYQIISRPLHFSFPLYSIEAQKVQAESDAMYLKNYLRLGMG
                     SLLNPYADFYANIPQSKRTAFGIHYRHLSAQADLEDYGESSFSDNLAEIYGKTIGKNL
                     AWNGGINFQRNMLHWYGFNPAKFSLPANSDTSITKQVYTRLCGNIELKKNVSNHDPFY
                     FDSRLDALWMEDKYGTSELNLSYFGNIQFTNSILSAR"
BASE COUNT          361 a          235 c          196 g          337 t
ORIGIN      
        1 aaaagaattt ggtatcataa cacggcttac acagtcggaa gccgcagcag aagctcaata
       61 tcatctttcc ttgataacat tcaaccgcaa cgatttatcc aatgcagaaa aaatgacttt
      121 tcagcttatc aataattatc cttccgctga ttattggaga gtgaagggat ttattctcct
      181 ttcagatatt tatgctgcaa atggtaatat ctttcaggct aggcaaactc tgcaaagcat
      241 catcgataat tataagggag aagacttgcg caaagaggct caaaaccgct tgaataatct
      301 cccccctgtt caataattct catttcgaca aacccattga aaatatgata agtaaaaaac
      361 ttttcttgaa attaagtacg accattgggg ttcttttgtg gcttcccatc gtcgaaaaaa
      421 gctttggcca gcatcctcag attgaagaag taaccatcat tggaagtttt actccccata
      481 tctccgatgc caataagatt ttgagaaatc ccatgttaag agatacgagt ataattctca
      541 aacccatgga atatcaaatt atctcgaggc ctttgcattt ttcctttccg ctttattcta
      601 tcgaggcgca aaaagttcaa gccgaaagcg atgcaatgta tctaaaaaat tatcttcgat
      661 taggtatggg aagcctcctc aacccttatg ccgattttta tgccaatatt ccgcaatcca
      721 agcgtactgc cttcggaatt cattacaggc atctttccgc ccaagccgat ttagaagatt
      781 atggtgaatc gtcatttagc gataatctgg ccgaaatcta tggaaaaacc atagggaaaa
      841 atcttgcatg gaatggagga ataaattttc aaaggaacat gcttcactgg tatggattta
      901 atccggccaa attttcactt ccggcaaatt cagatacaag tattacaaaa caagtatata
      961 cacgactatg tggaaatatt gaactaaaga aaaatgtttc taaccacgac cctttctatt
     1021 ttgattcgag gctcgatgct ttgtggatgg aagataaata tggcacttcc gaactgaatc
     1081 tctcatattt tggcaatatt caatttacca attccattct ttctgcccg
//
LOCUS       DAORPD010000716         1122 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_27489_length_1122_cov_1.865042, whole genome shotgun sequence.
ACCESSION   DAORPD010000716 DAORPD010000000
VERSION     DAORPD010000716.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27489_length_1122_cov_1.865042"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..849
                     /gene="deoD"
                     /locus_tag="PK976_08060"
     CDS             139..849
                     /gene="deoD"
                     /locus_tag="PK976_08060"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF004489.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0043096 - purine nucleobase salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HRC67881.1"
                     /translation="MGIHLDARVGEIAESVLLPGDPLRAKFLAEKYFDNPVCHNQIRG
                     MFGYTGYVNGKKISVQGTGMGVPSISIYVTELIQTYGCKNLVRIGTCGAIQPELKLGD
                     TVLAITASTDSHVNTLTFGGREYAPHADFELLKMAYNSGKALGHQVKVGSVFTTDSFF
                     VDDLEKEYEIWRRHGVLGVEMETSALYTLAARFRCRALSILTVSDQLVTGERATAQER
                     LTAFTAMMEIALACLKSL"
     gene            complement(937..>1122)
                     /locus_tag="PK976_08065"
     CDS             complement(937..>1122)
                     /locus_tag="PK976_08065"
                     /inference="COORDINATES: protein motif:HMM:NF014787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HRC67882.1"
                     /translation="YDGEDIEIGFNAKFLLEMLSNIDTPNIRIETSAPNRAGVIFPET
                     DENEVEDLLMLIMPVMI"
BASE COUNT          332 a          177 c          279 g          334 t
ORIGIN      
        1 cgaaataagc ccttggatga tggctttcgt gaatgcccga aaatggatta aaaataaaaa
       61 caagggataa tcctatacag tacagataaa attttgatga aataaaatcg aattgttaat
      121 cagataaatg aagattctat gggaatacat ttagatgcaa gggtaggtga gattgccgaa
      181 agtgtcttat tgcccggtga tccattaagg gctaaatttt tagccgaaaa atatttcgat
      241 aatccggttt gtcacaatca gataagagga atgtttggct atacaggtta tgtaaatgga
      301 aaaaagattt cggtacaggg aacaggaatg ggagttcctt ctataagcat atacgttacg
      361 gagcttatac agacttatgg gtgcaagaat ttagttcgca ttggtacatg tggtgccatt
      421 cagcctgaac tgaaattagg cgatactgta ttagccatta ctgcaagtac cgatagtcat
      481 gtaaatactt tgacctttgg agggagggaa tatgctcctc atgctgattt cgaattgttg
      541 aaaatggctt ataatagtgg aaaagcatta gggcatcaag tgaaggttgg aagtgtattt
      601 acaacggatt ctttttttgt ggatgatttg gagaaggaat acgagatttg gcgcagacac
      661 ggagttttgg gtgtagagat ggaaaccagt gcgctttaca cgctggcagc acgttttcgc
      721 tgtagagcct tgtcgatttt gacggtcagt gatcagttgg ttacgggcga acgtgccacg
      781 gctcaggaaa gacttactgc atttacagct atgatggaaa ttgccttggc ctgtcttaaa
      841 agcttatagg aatacgaaaa accccttatc attttttcga taaggggctg gagaattcag
      901 cagtttgtta ttgctgatgg gagagaggct aatttcttag atcattacag gcataatcaa
      961 catcagaagg tcttctactt cattttcgtc ggtttcggga aagataacac ctgcgcggtt
     1021 tggagccgac gtttcgattc tgatgttggg tgtatcgata ttggatagca tttcgagcag
     1081 aaacttggcg ttgaatccaa tttcgatgtc ttcgccatca ta
//
LOCUS       DAORPD010000717         1119 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_27602_length_1119_cov_2.384398, whole genome shotgun sequence.
ACCESSION   DAORPD010000717 DAORPD010000000
VERSION     DAORPD010000717.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27602_length_1119_cov_2.384398"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="PK976_08070"
     CDS             <1..396
                     /locus_tag="PK976_08070"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HRC67883.1"
                     /translation="VISEGARPIGGDFARKETGEVGYHSVKLSGAAERLMQELKEAGC
                     PYDMRATVLGHVQRGGVPIAYDRVLATQFGVKAMELVMEGKFGQMVSYRHPYITSVPL
                     KDAIKRYNFVDMDTALIKTARGVGICFGD"
     gene            complement(495..>1119)
                     /locus_tag="PK976_08075"
     CDS             complement(495..>1119)
                     /locus_tag="PK976_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRC67884.1"
                     /translation="QQALLKLLEGSIVNVPPQGGRKHPEQKFIQVNTQNILFIAGGAF
                     DGIENKIASRLRTNIVGFKKEPFTEIEKDNLLQYVSPPDLRAYGLIPELVGRFPVVCH
                     LSPLDANALKRILTEPKNALVKQYKKIFEMDKIQLSFDDEVLDFVVQKSIEFKLGARG
                     LRSIMESILKDLMFEAPSGKLAPTLHITLDYAQEKFETSGISRLKVA"
BASE COUNT          313 a          226 c          260 g          320 t
ORIGIN      
        1 gtgatttcgg aaggtgctcg tcctatagga ggcgattttg caagaaagga aaccggcgaa
       61 gtgggctatc acagcgttaa actttcggga gcagccgaac gcctcatgca agaattgaag
      121 gaagcaggct gcccttatga tatgcgagct accgttttag gccatgtgca gcgaggaggt
      181 gtgcctattg cctacgatcg cgtcctagct actcagtttg gagtgaaagc catggaactg
      241 gtaatggaag gaaaatttgg acaaatggtg tcgtaccgtc atccttacat tacctccgtt
      301 cccttgaaag atgccattaa aagatacaat ttcgtagata tggataccgc attaataaaa
      361 actgcgcgtg gtgtgggtat atgttttggc gattgaatca taggaaaata ttttctccaa
      421 atttaaagcc ctgagtctta ccgatcaggg ctttaaattt ctttgtcaga ataaacgtga
      481 atattcaatc caattcaggc caccttcaat ctgctgatgc cagaggtttc gaatttttct
      541 tgggcataat caagcgtaat gtgcagagta ggagcaagtt ttccagaagg cgcctcgaac
      601 attaaatcct taagaatgct ttccatgatg gaacgtaacc ctctggcacc aagtttgaat
      661 tctatggact tttgaacgac gaaatcaagt acctcatcat cgaagctcag ttggattttg
      721 tccatttcga atatcttctt atactgtttt accagagcat ttttaggttc ggtgaggata
      781 cgcttcaaag cattggcatc taagggtgat agatggcaaa ccacaggaaa acgacctaca
      841 agttccggaa taaggccata agctctcaaa tcaggaggag aaacatactg cagcaaattg
      901 tctttttcga tttcggtaaa tggctctttt ttaaagccta cgatattggt tctgagcctc
      961 gaggcaattt tattctcgat tccatcgaag gcgcctccgg caataaagag aatattctga
     1021 gtgttcacct gaatgaattt ttgctcggga tgtttgcgac ctccctgtgg aggaacattc
     1081 acaatgcttc cttcgaggag tttgagcaaa gcttgttga
//
LOCUS       DAORPD010000718         1119 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_27606_length_1119_cov_2.283835, whole genome shotgun sequence.
ACCESSION   DAORPD010000718 DAORPD010000000
VERSION     DAORPD010000718.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27606_length_1119_cov_2.283835"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..>1119
                     /locus_tag="PK976_08080"
     CDS             145..>1119
                     /locus_tag="PK976_08080"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC67885.1"
                     /translation="MLELFNIKKRLGDFLLEIPSYCLEEGEYFVFAGPSGAGKSQLLE
                     LIAGLSFPDEGKIIFDGIDITSLPTQKRKIGMVFQDMALFPHLTVKQNISFPMRAKKK
                     EPIYVKNEIMKLAHAMSIEKLLDHYPHQLSGGEARRVALARTLASQPRLLLLDEPLSE
                     VDTSLKVALRRLLRTINQQGVTIIHVSHDYEDALTLASKMAIINDGKIIQVGQPSEIF
                     LKPASPFVAQFAGIRNFFHCKFRSLHNSSLSLAVINDEKQIFITGTISSTHGSIIIPA
                     RDIVLSEQKLESSARNNFPGIVKEIIPTREGLEVIVDCSFPLAAIVSTA"
BASE COUNT          338 a          250 c          203 g          328 t
ORIGIN      
        1 atcaccctat gaccgctcct gtcttaatct ttgaaaggtt caacaattat ggactcaatt
       61 atgccaatcc cttagccgcc attttgattc ttgtaagcct tgttttcttc atccttttga
      121 gattgctcgc taaacccaca aaaaatgctt gaacttttta atattaaaaa aagattagga
      181 gattttcttc tggaaattcc atcctactgc ctcgaagaag gtgaatactt cgtatttgca
      241 ggcccttcgg gtgcaggtaa atcacaactg ctcgaactaa ttgcaggttt atcatttccc
      301 gatgaaggca aaatcatctt cgatggtatc gatattacca gcttgccaac ccaaaaacgt
      361 aaaattggaa tggtttttca ggatatggct cttttcccac atcttactgt aaaacaaaat
      421 atttcatttc ccatgagggc gaaaaaaaaa gaacccattt atgtgaaaaa tgaaataatg
      481 aaattagctc atgccatgag catagaaaag ctactcgacc attatcctca tcagctttcg
      541 ggtggagaag cccgtagagt tgctttggca agaaccctgg cttcacaacc acgcttactt
      601 ctactcgatg aacccctaag tgaagtggat acctctctga aggtagctct gcgaagattg
      661 ttacgaacta tcaaccagca aggggttacc atcatccatg taagtcacga ttatgaagat
      721 gctttgacgc ttgccagtaa aatggccatc attaacgatg gtaaaattat tcaagtgggg
      781 caaccttcag agatattttt aaagccagct tctcctttcg tagcccaatt tgctggaatt
      841 cgcaatttct tccattgcaa gtttcgatct ttacataact catcactttc cttggctgtc
      901 atcaatgatg aaaagcaaat atttattacc ggcacaatct cctcaacaca tggaagtatc
      961 attatacccg ccagagatat agtgttgtcg gaacaaaagc ttgaatcatc ggcacgcaat
     1021 aatttccctg gcattgtaaa ggaaataatt cctacacgtg aaggcctcga agtgatagta
     1081 gactgtagct ttccattagc agcgatcgta agcaccgct
//
LOCUS       DAORPD010000719         1119 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_27611_length_1119_cov_2.109962, whole genome shotgun sequence.
ACCESSION   DAORPD010000719 DAORPD010000000
VERSION     DAORPD010000719.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27611_length_1119_cov_2.109962"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(181..1011)
                     /locus_tag="PK976_08085"
     CDS             complement(181..1011)
                     /locus_tag="PK976_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67886.1"
                     /translation="MKHQHLALLLLFLSPLMGIAQKKYNPEVKLNKGDSITYLIQTNV
                     DMTQSMMGQEMKMNVSTQSKNIIAVSDIDKDKYYHLSQLSKEMTVKMKMDQMDSTMLV
                     PDITNLPVLVMSKKGTILRKSVSTNEKGQTMGNDFGDVTASSPFIEFPDRKISEGESW
                     ETLQNDTVNFMGGDLITNSKTVYTLIGTDEKEGMKCLKINHKTTADNEGTTTMQGMDF
                     LIEGTTIMEGTTWIDLKTAIPVFIDGTAESQMTLLLSGQQNMTIPLTYKTTFTKKLLK
                     "
BASE COUNT          319 a          195 c          208 g          397 t
ORIGIN      
        1 gattgataaa aatatttaca ggtaaagata gaaaattatc ggacaatgaa taaggtccat
       61 aaaaatatcg aaaactgaaa ccattattcc catatattac cagtgaattt gttctcaata
      121 aaaacaaaaa gaggccacac ttttggtaag agcctctttt caatatcaat agcgaacagc
      181 ttattttaag agtttcttag taaaagtggt tttgtaagtt aaggggatgg tcatgttttg
      241 ctggccgctg agtaaaagtg tcatctgact ttcggccgtg ccatctataa aaactggtat
      301 ggccgttttt aaatcaatcc atgttgttcc ttccatgatg gtagttcctt ctatcaaaaa
      361 atccattcct tgcatcgtag ttgtcccttc attgtcggcg gttgttttat gattgatctt
      421 caagcatttc attccctctt tttcatcggt tccaataaga gtatatacgg tttttgagtt
      481 ggtaatcaaa tcgccaccca taaagttcac ggtatcattt tgaagcgttt cccaactttc
      541 gccttccgaa atttttctgt cgggaaattc aataaaaggc gaactggctg taacatctcc
      601 aaaatcatta cccattgttt gacctttttc attggtcgaa accgatttgc gtaaaatggt
      661 tcccttttta ctcattacta atactggaag attggtaata tcggggacga gcatggtgct
      721 atccatctga tccattttca ttttaaccgt catttctttg cttaactggc ttaaatggta
      781 atatttatcc ttatcgatat cacttacggc aatgatgttt ttcgactgag tggaaacgtt
      841 cattttcatt tcctgtccca tcatgctttg agtcatatcg acattggttt gaattagata
      901 ggttattgaa tcgcctttgt tgagtttgac ctcgggattg tatttctttt gggcaatacc
      961 catcagagga ctaagaaaca atagaagcag agcaagatgt tggtgtttca taaattttag
     1021 gttttatgtg aaaagatcgt gtgaaacaat tcatgaatcg agggtgttgt tttaattcta
     1081 atgaaagata agttttcgaa tgattttgga cctttaatt
//
LOCUS       DAORPD010000720         1118 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_27653_length_1118_cov_2.415804, whole genome shotgun sequence.
ACCESSION   DAORPD010000720 DAORPD010000000
VERSION     DAORPD010000720.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27653_length_1118_cov_2.415804"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1112)
                     /locus_tag="PK976_08090"
     CDS             complement(<1..1112)
                     /locus_tag="PK976_08090"
                     /inference="COORDINATES: protein motif:HMM:NF020249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PglZ domain-containing protein"
                     /protein_id="HRC67887.1"
                     /translation="MVSQKTSTAYQQAFSEIGMEISSSLDFEGWMEIYRRIINWELRL
                     QTTRDEGMAEVLRMQKEEANQVFSRFYETNYPVWLTGKSTSKPVLSHTLIRQKLFPLL
                     EHGSPLFFILIDNLRYDQWKTIQPVIEEFYRVERDELYYSIIPTTTQFARNALFSGLL
                     PSEIEKKYPKWWLNDWEEGTKNQYEEELFGELLKRFGRDVKFSFNKILNLSAGRKLVD
                     NLPNLLSNRLNIIIYNFVDMLSHARTEMEIIRELANDEPAYRSLTLSWFQHSPLHDIL
                     RFLSMKKIDVAITTDHGSIRVQNAVKVVGDRETNTNLRFKAGKNLNFNKKECFYMRNP
                     AEIFLPRQNVSTSYIFARSSDFFAYPNNYNYFANYYR"
BASE COUNT          326 a          206 c          244 g          342 t
ORIGIN      
        1 cggtaataat tggcgaaata attatagttg ttaggatagg caaaaaaatc gctgcttcgt
       61 gcgaatatat aggaagtgct cacattttgg cgagggagga aaatttctgc aggattgcgc
      121 atataaaagc attctttttt attgaaattc aaattttttc cagctttgaa tcgcagattg
      181 gtattggttt ccctgtcgcc cactacttta acagcatttt gaaccctgat agaaccatga
      241 tctgtagtga tggccacgtc gatttttttc atggataaga atctcagaat atcgtggagg
      301 ggagaatgtt ggaaccatga aagagtgagg gagcggtaag cgggttcatc gttggcaagt
      361 tcacgtataa tttccatttc ggtacgggca tgagaaagca tatctacaaa attgtaaata
      421 atgatattca agcgattgga aaggagatta gggagattat ccacgagttt gcgtccagcc
      481 gacagattaa ggattttatt aaacgaaaac ttgacatcgc gaccgaatcg tttgagcagc
      541 tcaccgaaaa gctcttcctc atattgattt ttggtgcctt cttcccagtc gttgagccac
      601 catttaggat attttttttc gatttcggat ggtaaaagtc ctgaaaataa ggcattacgg
      661 gcaaattgtg tagtggtggg aataatactg tagtatagct catcacgttc cactctgtaa
      721 aattcttcaa ttacaggctg aatggttttc cattgatcat atcgtaaatt atcgatcaat
      781 atgaagaaaa gtggagagcc gtgttccaga agagggaaga gtttctgcct aataagcgta
      841 tgcgaaagaa caggtttgct ggttgatttg ccggttaacc acacaggata atttgtttcg
      901 taaaaacgag aaaacacttg atttgcttct tctttctgca ttctcagaac ctccgccatg
      961 ccttcatcac gagtagtttg tagacgaagc tcccaattga tgattcggcg ataaatctcc
     1021 atccaacctt caaaatcgag cgaactactt atttccatac ctatttcgct aaaagcctgc
     1081 tgataagccg ttgaggtctt ttgacttacc aagtcttt
//
LOCUS       DAORPD010000721         1117 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_27695_length_1117_cov_2.597928, whole genome shotgun sequence.
ACCESSION   DAORPD010000721 DAORPD010000000
VERSION     DAORPD010000721.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27695_length_1117_cov_2.597928"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="PK976_08095"
     CDS             complement(<1..571)
                     /locus_tag="PK976_08095"
                     /inference="COORDINATES: protein motif:HMM:NF012274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HRC67888.1"
                     /translation="MAKARIAINGFGRIGRLTFRALLEKEEVEVVAINDLTDSKTLAH
                     LLKYDSVHRRFPGTIEVDGDYLVVNGNKIRIYAEKDPLNLPWKDLSIDVVVESTGVFR
                     TREKMSKHLTAGAKKVALSVPADNAEDVDITVVIGVNDHQIRPEHKLISNASCTTNCL
                     APVAKILHEKFGIVRGLMNTVHSYTNDQVI"
     gene            802..>1117
                     /locus_tag="PK976_08100"
     CDS             802..>1117
                     /locus_tag="PK976_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67889.1"
                     /translation="MLRENPILKLFIPLITGILLSIYFSRIRFSPILCGIIFLIAAIY
                     LLFFYIRLSYKNQWIYGLMIHILMFLSGVSVVSLRELSFKHEHFSSDKTSEYFQAIIE
                     DYP"
BASE COUNT          326 a          229 c          169 g          393 t
ORIGIN      
        1 gaataacctg atcattcgtg taagaatgaa ctgtattcat caatcctctt acgataccga
       61 atttttcatg cagaatctta gccacggggg caaggcagtt cgtcgtgcac gaagcattcg
      121 aaatgagttt atgctcaggc ctgatttgat gatcattcac tcctattacc acggtaatat
      181 cgacatcttc cgcattgtcg gctggtaccg ataaagccac tttcttagcg cccgcagtga
      241 gatgtttcga catcttttca cgtgtacgaa atacacctgt cgattcaaca actacatcga
      301 tgctcaaatc tttccatggc aaattcaagg ggtctttttc agcatatatg cgtatcttgt
      361 ttccattaac tacaagataa tcaccatcca cttcgattgt accaggaaac ctgcgatgaa
      421 ctgaatcgta tttcaacaag tgggcaagtg tttttgaatc agtcaggtcg tttatagcta
      481 caacttctac ttcttctttt tcgagcaaag ctctgaaagt gagccttccg atacggccaa
      541 atccattaat tgcaattctt gctttagcca tttttatatg attttaattt tttcgctgca
      601 aagataacaa cttcatttct atcgttcatg tgattttgcc tctctttcat aaataaatga
      661 ttaacaatac cttaatttca ggttccttca aatttaacct tttcatttat taaaaaatat
      721 ctctcttaaa aacaaaattt tacgccctct gaatatttaa acaaaaaatt catactttcg
      781 ttgatgtatt aacctgaaac catgctaaga gagaatccta tattaaagct ttttatccct
      841 ctgataacag gaattctttt aagtatttat ttctcgagaa taagattctc tcccatttta
      901 tgtggaataa tattcttaat tgcagctata tatctgcttt tcttctacat ccggctttct
      961 tacaagaatc agtggatcta tggacttatg attcatattt tgatgtttct atctggagta
     1021 tccgtagtta gccttaggga attatcattc aaacatgaac atttttcatc ggacaaaaca
     1081 tcggaatatt ttcaggctat aattgaagat tatcctc
//
LOCUS       DAORPD010000722         1117 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_27696_length_1117_cov_2.452919, whole genome shotgun sequence.
ACCESSION   DAORPD010000722 DAORPD010000000
VERSION     DAORPD010000722.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27696_length_1117_cov_2.452919"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1117
                     /gene="feoB"
                     /locus_tag="PK976_08105"
     CDS             <1..>1117
                     /gene="feoB"
                     /locus_tag="PK976_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HRC67890.1"
                     /translation="SPEELFVRVYLTENMPDIVLNVVDASNLERNLFLTTQLIDMDIK
                     VVIALNMFDELEKKGDRLDYKYLGKMLGIPIVPTVATKGEGIEELLQKIIDVYEDKDP
                     IVRHIHINYGSIVEKAIKQIQDVIWQNPAITDKLSSRYLAIKLLEGDKTILSELEKYD
                     NYPQIESTTRQAIQSLEKEYGDSCETVITQLKYGFIEGALKETYHLSKQKHKEKQQTL
                     DNLFLHKFWGYPIFLFFVWLMFQSTFSLGKYPTQWLESGINLLGSWLGRIMVPGPLCD
                     LLVNGIIGGVGNVLVFFPNILILFFFVSILEDTGYMSRAAFLMDKLMHKIGLHGQSFI
                     PLVIGFGCNVPAILATRTLKNRKDRILTIIIIPFMSC"
BASE COUNT          345 a          200 c          227 g          345 t
ORIGIN      
        1 tttcgcctga agaactcttt gtcagagttt atttgacgga aaatatgcct gatattgtgc
       61 tcaatgtggt agatgcttcc aatctggaac gtaatttatt ccttaccact cagctcatcg
      121 acatggatat aaaagttgta attgctttga atatgtttga tgagctcgaa aaaaaaggtg
      181 ataggcttga ttacaaatat ttagggaaaa tgcttggaat ccccattgtt ccgactgttg
      241 ccaccaaagg tgaaggcatc gaagagctcc tgcaaaaaat aatagatgtt tatgaagata
      301 aagatcccat tgtcaggcac atccacatta attatggatc tatagtcgag aaagccatca
      361 aacaaattca ggatgtgatc tggcaaaatc cagccattac ggataaatta tcttcccgtt
      421 atctggctat caaacttttg gaaggtgata aaaccattct ctccgaactg gaaaaatatg
      481 acaattatcc tcaaatcgaa tctactacga ggcaagctat tcaaagcctc gaaaaagaat
      541 atggcgatag ttgcgagacc gttattactc aactaaaata tggattcatc gaaggagcct
      601 taaaagaaac ctatcatcta tcgaagcaaa aacataagga gaagcaacaa actttagata
      661 atttgtttct gcataagttc tggggttatc ctatttttct gttcttcgta tggttaatgt
      721 ttcaatcgac cttcagcctt ggaaaatatc ctacccaatg gctggaaagt ggaattaact
      781 tactgggtag ctggctgggg aggataatgg tcccaggacc attgtgtgac ttattggtga
      841 atggaatcat tggtggagta ggaaatgtgc tcgtcttctt tccgaatatt cttattttgt
      901 ttttcttcgt ttccatcctc gaggatacag gctatatgtc gagagcggca tttttaatgg
      961 ataaattgat gcacaaaatt ggattgcatg ggcaatcatt tattccactg gtaataggat
     1021 ttggttgcaa tgtgccggct attttggcca cgcgaacatt aaaaaatcgt aaagaccgaa
     1081 ttttaaccat tatcattatt ccgtttatgt cttgcag
//
LOCUS       DAORPD010000723         1117 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_27701_length_1117_cov_2.137476, whole genome shotgun sequence.
ACCESSION   DAORPD010000723 DAORPD010000000
VERSION     DAORPD010000723.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27701_length_1117_cov_2.137476"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="PK976_08110"
     CDS             <1..441
                     /locus_tag="PK976_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HRC67891.1"
                     /translation="KDIQIYLNTPGGSVYAGLGIYDTMQYIAADVATICTGMAASMGA
                     VILCAGAKGKRTALKHSRILIHQPMGGAEGQASDIEITAREIQKLKKELYEIIAYHTG
                     QPFEKIWQDADRDFWMTSYEAKEYGMIDEVLFRERNTKDSSNQA"
     gene            491..>1117
                     /locus_tag="PK976_08115"
     CDS             491..>1117
                     /locus_tag="PK976_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRC67892.1"
                     /translation="MAKTLEYCSFCGRSKKEVNLLISGINANICDSCIEQARDIVLQE
                     ITSARKKKVHSKKIYKPAEIKAYLDQYIIGQEEAKKVLSVAVYNHYKRISQPISQNNI
                     DDVEIEKSNIIFVGETGTGKTLLAQTIARLLNVPFCIADATVLTEAGYVGEDVESILA
                     RLLQAADYDIATTEMGIVFIDEIDKISRKSDNPSITRDVSGEGVQQALL"
BASE COUNT          369 a          214 c          253 g          281 t
ORIGIN      
        1 aaagacattc aaatctattt gaacacgcct ggaggttcgg tatatgcggg attagggatt
       61 tatgatacca tgcaatatat tgcagccgat gtggccacta tctgtacagg catggctgcc
      121 tcgatgggtg cagtaatcct atgtgctggt gccaaaggca aaagaacagc cctcaagcac
      181 tcacgcatcc tgattcatca acctatggga ggtgccgaag gacaagcttc cgatatagaa
      241 atcactgcca gagaaattca aaaattaaaa aaggaactgt atgaaattat tgcctatcat
      301 acaggccaac ctttcgaaaa aatctggcaa gacgccgacc gtgatttctg gatgacttca
      361 tatgaagcca aggaatatgg aatgatcgac gaagtattgt tcagggaaag gaatacgaaa
      421 gatagttcaa atcaagccta aggattcttt ttctttggct cgagatattt tgtacaaaag
      481 gaagaaatcg atggcaaaaa cattggaata ctgttcattt tgcgggcgaa gtaaaaaaga
      541 agtcaatctg ctcatcagtg gaataaatgc caatatttgc gatagttgta tagaacaggc
      601 ccgtgatatt gttttgcagg aaataacttc cgcacgaaag aagaaggttc attcgaagaa
      661 aatctataaa cctgcagaaa tcaaagccta cctcgatcaa tacattatcg ggcaggaaga
      721 ggccaagaaa gtgctctcgg ttgcggttta caatcattat aagagaattt cacaacctat
      781 cagccagaac aatatagatg atgtagaaat cgaaaaatcg aatatcatct ttgtagggga
      841 aaccggtaca gggaagacac ttctggctca aaccatagcg cgcttgctca atgtaccttt
      901 ttgcatagct gatgccactg ttttaaccga agccggttat gtgggagaag atgtcgagag
      961 catattagct cggttgctgc aagccgccga ttacgatata gctaccacag aaatgggtat
     1021 tgtttttatt gacgaaatcg ataaaatctc tcgtaaaagc gacaatccaa gcattacacg
     1081 tgatgtatcg ggtgaagggg ttcaacaagc tttgctc
//
LOCUS       DAORPD010000724         1117 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_27704_length_1117_cov_2.009416, whole genome shotgun sequence.
ACCESSION   DAORPD010000724 DAORPD010000000
VERSION     DAORPD010000724.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27704_length_1117_cov_2.009416"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(186..>1117)
                     /locus_tag="PK976_08120"
     CDS             complement(186..>1117)
                     /locus_tag="PK976_08120"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HRC67893.1"
                     /translation="PFFFYGAGKITDKITAGLGIYTPYGSKTDWPDDWSGRFLIQNIA
                     LSSIYFQPSISFQILDNLSIGAGLAYVMGSVDLQRALPYNDRSFVKLNGNASAFGFNI
                     GVLFKPAESMHIGIDYRSKIEVKLDDGDANFRIPASLVSIITIENKFSSSLPLPANLD
                     CGLSWQITQKLLFSFEINWVLWNCYDSLTFTFIENGDMLNSINPRLYSNSIIPRFGLE
                     YRIQPKLKIRTGAYYDNSPANDEYFTPETVTLNTLGFTFGLSYHPIENLNFDFSFVQL
                     MGIEGEKKYSPGNFVGKYRSSASIPGIGISYSF"
BASE COUNT          375 a          221 c          211 g          310 t
ORIGIN      
        1 ttatataaag ccccatcggc ataacccgaa gtaagggaat tcccaagtgc tacataatgg
       61 tagaaatctg cttcacctct gctgggagca aattcttcgg ttttgggttc acacgccccc
      121 atcaaaactg aaaagaaaaa aatggagtaa agagcttgta ttttcatcgt ggagaaattt
      181 ttaatttaaa aactataaga gattcctata cccggtatag aggccgatga tcgatacttg
      241 cctacaaaat tacccggact atacttcttt tcgccttcta tacccattaa ttgtacaaac
      301 gaaaaatcaa aattcaaatt ttcaattgga tgataactga gcccaaaagt aaatccaaga
      361 gtattcagtg ttacggtttc gggagtaaag tactcatcgt tggctggaga attgtcgtaa
      421 taagcacccg tacgaatttt taatttgggc tgaatccgat attccaatcc aaatcgggga
      481 atgattgaat tactatataa acggggattt atactattca acatatcacc attttcgata
      541 aaagtgaatg tcagtgaatc gtaacaattc cacaacaccc agttgatttc gaatgaaaaa
      601 agtaacttct gtgttatctg ccatgataat ccacaatcga gattcgctgg cagggggaga
      661 gaggaagaaa atttattttc tattgtgata atagaaacaa gagaagcagg tattcgaaag
      721 tttgcatcgc catcgtccaa tttaacctca atctttgaac ggtaatctat tccaatatgc
      781 attgattctg caggcttgaa tagaacgcca atgttaaatc caaaagccga agcatttcca
      841 ttgagcttga caaatgatcg atcgttatat ggcagtgctc tctgcaggtc aaccgaaccc
      901 atcacatagg ccaggccagc gccaatactt agattatcta gaatctgaaa acttatagaa
      961 ggttgaaaat aaatggagct cagtgcaata ttttgaatga gaaagcgacc gctccaatca
     1021 tcaggccagt ctgtcttgct gccgtaagga gtataaatac ctaaccctgc agttatctta
     1081 tcggtaatct taccagctcc atagaaaaaa aatggca
//
LOCUS       DAORPD010000725         1116 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_27739_length_1116_cov_2.508954, whole genome shotgun sequence.
ACCESSION   DAORPD010000725 DAORPD010000000
VERSION     DAORPD010000725.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27739_length_1116_cov_2.508954"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="PK976_08125"
     CDS             complement(<1..329)
                     /locus_tag="PK976_08125"
                     /inference="COORDINATES: protein motif:HMM:NF024790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HRC67894.1"
                     /translation="MRKESFIFYAMLCAAILLARVPCIGPFFRVINKMVHETGHALIV
                     LLFSGKVYKIELFADLSGTTVSSTKSRFSRIIVSLTGYLFSVLMAWVMFYLLYHQQQR
                     AVIILFTS"
     gene            515..>1116
                     /locus_tag="PK976_08130"
     CDS             515..>1116
                     /locus_tag="PK976_08130"
                     /inference="COORDINATES: protein motif:HMM:NF014930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67895.1"
                     /translation="MSKQRFQNQENNKSTLHFTFSAIDAKANYEETRRQVGYVNRKQA
                     TQEDYDRIGFKSGLEVHQQLFTEKKLFCNCPAGLYHADDDYDAEVIRHMRPTLSELGE
                     YDGTALMEFKTRKNIIYRIKYDSACTYEVDDTPPFPMNRQALRIAIEISLLSKLNIVG
                     EVHITRKQYLDGSIPTGFQRTAIVGVEGEIPLRNKKIRLI"
BASE COUNT          371 a          223 c          218 g          304 t
ORIGIN      
        1 gatgtaaaaa gtatgattac agccctttgc tgctgatgat acaataaata gaacattacc
       61 catgccatca aaacagaaaa aagatatcct gtcagcgaca caatgattcg tgaaaaccta
      121 ctctttgtgc tcgagacggt agttcccgat aaatctgcaa ataattcgat cttatagact
      181 ttgccactga ataaaagcac tatcagggca tgacccgttt cgtgtaccat cttattgata
      241 actctgaaaa aaggtccgat gcagggaaca cgagcgagaa ggatggctgc gcaaagcatt
      301 gcataaaaaa tgaaagattc ttttctcatg atcgaacttt ctatacgcaa aattaaggag
      361 agctcaaaaa aaatctcgag gaaaaggtaa aagaaatgaa attcataatg tattttctgt
      421 gagtgttttc gatcggcttc tcatgctttt cattgcgaat tccttgtggt cagattgaga
      481 attcctacat ttgaagtctc aaacgtcttt tctaatgtca aaacaacgtt ttcagaatca
      541 agaaaataac aaaagtacgc tgcattttac tttttcagcc atcgatgcaa aagccaatta
      601 tgaagaaact cgtcgacagg ttggttatgt aaaccgtaag caagccactc aggaagacta
      661 cgatcgcatt ggattcaaat cgggtctcga agttcatcaa caattgttca ccgaaaaaaa
      721 attattctgc aattgcccgg caggccttta tcatgccgat gatgattatg atgccgaagt
      781 gattcggcac atgcggccaa ccttgagtga acttggtgaa tatgatggta ctgccttaat
      841 ggaattcaaa acgcgaaaaa atattattta tcgaatcaaa tatgacagtg cctgtacgta
      901 tgaggtggac gatactcctc ctttcccaat gaaccgtcag gctctgagaa ttgccatcga
      961 aatttcactt ctatccaaat taaatatcgt tggcgaggta catattacac gaaaacaata
     1021 tctcgatggt tccataccca caggatttca gcgcacggcc attgtgggag tggaaggcga
     1081 aattccttta cgtaacaaga aaatcaggct tataca
//
LOCUS       DAORPD010000726         1115 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_27797_length_1115_cov_2.019811, whole genome shotgun sequence.
ACCESSION   DAORPD010000726 DAORPD010000000
VERSION     DAORPD010000726.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27797_length_1115_cov_2.019811"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="PK976_08135"
     CDS             <1..513
                     /locus_tag="PK976_08135"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HRC67896.1"
                     /translation="GIEKARLYTQPEHVAYAYEQLLKISPNFTIAASFGNVHGVYKPG
                     NVILRPIILHNSQQYIEKKLNTPPKPVNFVFHGGSGSSQDDIREAISYGVVKMNIDTD
                     TQWAQWCGILNYYKKYSGYLQSQIGNPEGEDKPNKKYYDPRKWMREAEISMVNRLKQA
                     FDDLNALNRF"
BASE COUNT          358 a          192 c          240 g          325 t
ORIGIN      
        1 ggcattgaga aggcccgcct ttatacccaa cccgaacacg tcgcctatgc ttatgagcag
       61 cttctaaaaa tcagtccgaa ttttaccata gccgcttcat tcggtaatgt gcatggcgtt
      121 tataagccgg gtaatgttat attgaggcct atcatccttc ataattctca acaatacatc
      181 gagaaaaaac tcaatactcc gcccaaaccc gtaaattttg tgtttcatgg tggctctggt
      241 tcgagtcagg acgacattcg tgaagccatt agctatggtg tagtcaaaat gaacatcgat
      301 accgatactc aatgggcgca atggtgtggt atcttgaatt attataagaa atatagcggg
      361 tatttgcaaa gtcagattgg aaatcccgaa ggtgaggata aacccaataa gaagtactac
      421 gatccacgca aatggatgcg cgaggccgaa atatctatgg taaaccggtt gaaacaagct
      481 ttcgacgacc ttaatgcatt gaatagattt tgaaaattgt tttgaaagct aagcttctaa
      541 atataaaata gcaaaattat ttgcttcctg ctcttcttcc atatatcgta caagaggaaa
      601 gaagacattt tacgagttat aaaagggcag agacaatggc aaagtgtgat ggaaaggttg
      661 ttttaatggt cgtggatttg ccttataatt atgaacttct cataaatatt attagatttc
      721 ggcgaggatt tagatagtat gggttgagaa tggccctaag gccactcttt atgtagaaga
      781 tgaaaacagc tcctttagat atttttcgag gaatattgat ttacctattg aatgtttatg
      841 ttggcatgaa aacgataaag cacttgaacc cgcaattgtc tattatcgct cgaatggctt
      901 atgtttatga aggtgaacag gaatgaagtc gtgaaaaagg cttgcatgat tatatcctcg
      961 caattatgcg aagggaaatc cttttcgaaa agatggaaaa ttttcagagg tgatcgcaat
     1021 tagaagaatc ttatctcgac ataacactaa tcaagtcttt tgggcgaata ctcataacag
     1081 gaataggaga acggtgaatc atttgttcgg catag
//
LOCUS       DAORPD010000727         1114 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_27841_length_1114_cov_1.844193, whole genome shotgun sequence.
ACCESSION   DAORPD010000727 DAORPD010000000
VERSION     DAORPD010000727.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27841_length_1114_cov_1.844193"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..>1114)
                     /locus_tag="PK976_08140"
     CDS             complement(86..>1114)
                     /locus_tag="PK976_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67897.1"
                     /translation="ELENYQQHVVAIRSWGAVTFLVKKSPFSTEEIINVHRFCENRMF
                     DPLILPETQAIDRDKFNMLQDMTFFTNVDQLLSPHKNTFIRQYPYRIYPTTDDRPFFF
                     QFIRFKQIPALISSIQEKNFPFLDIGYVLVILTFIQIIILAAIFILFPLSFRKWKGKN
                     KKWVFVYFSILGLAYMFLEIVFIQQFTFYFGEPTYATAATLTILLFTSGLGSYYSSGL
                     QNTPKIRMALPIIVAAILILYTFVLSPILSATIGAVLPLKILIATILLGILGFFLGMP
                     FPIGIDYLSGKRDDNIPWAWAFNGYFSVISTVLATIISVEAGYLWLVWIAAFLYASAG
                     ISNLWLKE"
BASE COUNT          390 a          201 c          227 g          296 t
ORIGIN      
        1 gtcttatcaa atgtggaaat tttttaacga ctgaatgatg atcttgcttt ccgcgaggca
       61 acagcctaaa aatttcaaaa tcaaatcatt ccttgagcca taaattggag atgcctgccg
      121 aagcatagag gaaagctgca atccatacta accataggta acccgcttca actgaaataa
      181 tggttgctaa aacggtactg attaccgaaa agtaaccgtt aaaagcccac gcccaaggga
      241 tattatcatc cctttttccc gataaatagt caatgccgat agggaaaggc attcctaaga
      301 agaaacccaa gatgcctaat aaaatcgttg caatcagtat tttaagaggt aaaacagctc
      361 cgatggtagc cgacaaaata ggagataaga caaacgtata taaaatgagt attgcagcaa
      421 ctatgatggg taatgccata cgtatttttg gggtattttg cagaccagat gaataataac
      481 tacctaatcc tgaggtaaat agcaatatag tgagagttgc agctgttgca taggtaggct
      541 cgccaaaata gaatgtaaat tgttgaataa aaactatttc cagaaacatg tatgccaagc
      601 ccaatatgct gaaatacacg aaaacccatt tcttgttttt acctttccat tttctgaacg
      661 atagggggaa aagaataaaa atagcggcga gaatgataat ttgaataaag gtaagaatta
      721 ccaggacata gccgatatcc aaaaaaggga aatttttttc ttgaatggaa gaaatcagcg
      781 ccggaatttg cttaaaacgg ataaactgaa agaaaaacgg ccgatcgtcg gtggtcgggt
      841 atatccgata aggatattgt cggataaatg tatttttatg gggagatagc agttggtcaa
      901 cgttggtaaa aaaagtcata tcctgaagca tgttaaattt atcacgatcg atcgcttgag
      961 tttcgggcaa gatcagcgga tcaaacatcc tgttttcgca aaacctatga acgttaatta
     1021 tctcttccgt gctaaatggc gattttttta ccagaaaagt aaccgcaccc caacttcgaa
     1081 tagcaacaac atgttgctgg taattctcga gttc
//
LOCUS       DAORPD010000728         1112 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_27911_length_1112_cov_3.307474, whole genome shotgun sequence.
ACCESSION   DAORPD010000728 DAORPD010000000
VERSION     DAORPD010000728.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27911_length_1112_cov_3.307474"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1112)
                     /locus_tag="PK976_08145"
     CDS             complement(<1..>1112)
                     /locus_tag="PK976_08145"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HRC67898.1"
                     /translation="KLTVYTKGAIDNLLGICTHVVANGEVLPMTDQWKEEFNQAVIRM
                     TASALRTLGAAYKPVDKPTENSEMEKDLILIGIVGLMDPPRPEIKHSIEKAKKAGITT
                     VMITGDHKNTAFAIAKELGIAETIQQVITGLEIDEMSEEVFSEKIMQYRVFARVSPEH
                     KVKIVHAFQSHGYIVSMTGDGVNDAPSLNAADIGIAMGVTGTDVAKDSSDMILTDDNF
                     STIVLAIEQGRNIYNNIRKAVLFLLTCNLGEVITMFVALISGMAAPFLATQLLWINLI
                     TDTLPALALGIDPPDPDIMEEKPRDPNESFFAHGEGLRIILGGILIGSISIIAFWFGY
                     FEHGFSPFSQNVPQKVIEYARTLAFITIIACQLIYSL"
BASE COUNT          310 a          253 c          216 g          333 t
ORIGIN      
        1 agggaataaa tcaactgaca ggcaatgatt gttataaaag ccaaggttcg tgcatattct
       61 ataacttttt gaggcacgtt ttgactgaat gggctgaagc catgttcaaa atatccgaac
      121 cagaaagcta tgatagaaat cgatcctata aggatgcctc ccagaatgat gcgcaagcct
      181 tccccatgag cgaagaaact ttcgttagga tcacggggtt tttcttccat tatatcggga
      241 tcgggcggat ctattcctaa agccagcgca ggcaatgtat ccgtaataag attgatccac
      301 aaaagttgag tagcaagaaa aggtgcagcc atccccgaaa ttaatgctac aaacattgta
      361 atcacctctc caagattaca tgtcaataaa aacagaacag cctttctgat attattgtaa
      421 atattacggc cttgctcgat tgctaatact atggtagaaa aattgtcatc cgtcagtatc
      481 atatccgaag aatccttggc tacgtcagtt cctgtaactc ccatggcaat gccaatatcc
      541 gccgcattca acgaaggtgc atcatttacg ccgtcgcctg tcatcgatac aatgtaacca
      601 tgtgattgga aagcatgtac aattttcacc ttatgttcgg gcgaaaccct agcaaatact
      661 cggtattgca taattttttc cgagaatacc tcttccgaca tttcgtctat ttcgaggcca
      721 gttatcactt gttgtattgt ttcggctata cctaattctt tggctattgc gaaggctgta
      781 tttttatggt cacccgttat cataactgtg gtaattccag cctttttggc cttttcgata
      841 gaatgtttta tttctggacg aggcggatcc atcaacccaa cgataccaat cagaatcaaa
      901 tctttctcca tttccgaatt ttctgtgggc ttatcaacgg gcttataggc tgcacccaag
      961 gttctcaaag cagatgccgt catccttatc acagcctgat tgaattcttc tttccattgg
     1021 tcggtcatag gcaatacttc cccattggca accacgtggg tgcagattcc taacaaatta
     1081 tctatggcgc ctttggtata cacagtgagc tt
//
LOCUS       DAORPD010000729         1112 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_27924_length_1112_cov_1.924314, whole genome shotgun sequence.
ACCESSION   DAORPD010000729 DAORPD010000000
VERSION     DAORPD010000729.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27924_length_1112_cov_1.924314"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..867)
                     /locus_tag="PK976_08150"
     CDS             complement(<1..867)
                     /locus_tag="PK976_08150"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008765 -
                     UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HRC67899.1"
                     /translation="MNKTLKDIIVNISAESIIGVLDQPVNRVVLDSREADEGSVFVAI
                     KGQKTDGHEFIEKAIENGATVIVCQSLPIVLDPRVTYLQVKNTSLAVGPIASAFFDYP
                     SRRLKLVGVTGTNGKTTITTLLYRLFKSLGYTSGLISTIENRIDNEILPAHLTTPNAV
                     SLNALLNSMAKAGCSHVFMEVSSHAIAQGRIYGQEYTGGIFTNLTHDHLDYHKTFSEY
                     LNAKKMFFDNLGNTAFALSNADDPNGEVMLQNTKARKYFYGLKNLADFHSRILEMDLE
                     SMLLHIDGYETWF"
     gene            complement(869..>1112)
                     /locus_tag="PK976_08155"
     CDS             complement(869..>1112)
                     /locus_tag="PK976_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HRC67900.1"
                     /translation="TNEFGYCERHNHRVSTKILSFSDKSIPNLKGMPAKDAVYLLESM
                     GLKVILEGKGKVNAQSLPAGTQVKTGSVITLTLSHS"
BASE COUNT          305 a          220 c          227 g          360 t
ORIGIN      
        1 gaaccaagtt tcatatccat cgatatggag cagcatgctt tccaaatcca tttcgagtat
       61 gcggctgtgg aaatcggcta aattttttag accgtagaaa tacttccgag ctttcgtatt
      121 ttgcaacata acttctccat tcggatcatc ggcattactc aatgcaaaag ccgtgttgcc
      181 caaattgtcg aaaaacatct ttttggcatt cagatattcc gaaaaggtct tgtgataatc
      241 cagatgatcg tgtgtgagat tggtaaaaat gcctccagta tattcttgtc cgtaaatacg
      301 tccctgagct atggcatgcg aacttacttc catgaataca tggctgcaac cggcttttgc
      361 catactgttc aataaagcgt tcaatgaaac ggcgttgggt gtagtgagat gggcaggtag
      421 aatttcattg tcgatcctgt tttctatggt cgagataagc ccactagtat atccgaggct
      481 cttgaacaag cgatataaca gagtggttat tgtggtttta ccattagttc cggttacacc
      541 tacaagtttg agccttctgg aaggataatc gaaaaatgct gaagctattg gcccaactgc
      601 caatgaagta tttttaactt gaagataggt tactcgaggg tctaaaacga taggcaaaga
      661 ttgacaaaca atgaccgttg ctccgttttc tatggccttt tcgataaatt catgcccgtc
      721 tgttttttgt cctttaattg ctacaaaaac acttccttca tcggcttctc gcgaatctaa
      781 tactacccgg tttacaggct ggtctaaaac tccaataatg gattctgcac ttatgttaac
      841 aataatatct tttagtgttt tattcatctt aagagtgtga taaggtgagg gtgattacac
      901 ttcctgtctt tacttgggtg cctgcgggta atgattgtgc attaaccttg cctttgcctt
      961 cgagaattac ttttagtccc atagattcga gtagataaac agcatctttt gctggcatcc
     1021 ctttcaaatt gggtatggat ttatctgaaa aagataaaat cttagttgaa acacgatgat
     1081 tatggcgctc gcaatagcca aattcattgg tt
//
LOCUS       DAORPD010000730         1111 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_27954_length_1111_cov_2.716856, whole genome shotgun sequence.
ACCESSION   DAORPD010000730 DAORPD010000000
VERSION     DAORPD010000730.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27954_length_1111_cov_2.716856"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(259..>1111)
                     /gene="secF"
                     /locus_tag="PK976_08160"
     CDS             complement(259..>1111)
                     /gene="secF"
                     /locus_tag="PK976_08160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HRC67901.1"
                     /translation="DFTGGRTYVVRFDRDVNVPEIRELLANEFGEAPEVKTYGPSNQV
                     KITTKYLIDEEGREVDSLIDRKIYNALRGLYANEINFSHFASDLNQSGKLIGILTSQK
                     VGPTIAYDIKRNAIMAVIFALLGIFIYIAARFNKWQFGLGGVISLFHDTIIIIGLFSL
                     FHGILPFNLEVDQSFIAAILTIIGYSINDTVVIFDRIREYNKLYPKRDLKINMNAAIN
                     STLARTMNTSGTTLVTLLMMFIFGGETLRGFLFALILGIVFGTYSSVFNASAIAYDTM
                     KNKWKKK"
BASE COUNT          359 a          215 c          225 g          312 t
ORIGIN      
        1 agcggggatt tgcttaggcc cgaacaatat aagggcaacc agtaaaataa gaataagctc
       61 tcccgaacta atatcgaaaa tcagaaaagg gacttgcata agtattttca ttaaagaatc
      121 gaaaataaat tacaaagcta caacatacct caaataaatg gaagatttat atttgattcg
      181 tcgagcattc tggtcgataa aaagcaaaat acccgatgac atcagccagc gggtattttc
      241 caagatatga attaatagtt attttttctt ccatttgttt ttcatggtat cgtaagcaat
      301 ggcactggca ttgaacactg aagaataggt tccgaaaacg attccaagaa ttagggcaaa
      361 caggaaacct ctcagtgttt cacctccaaa gataaacatc ataagcagag ttaccaatgt
      421 agtacccgaa gtattcatgg ttcgggcaag agtagaattg atggcggcat tcatgtttat
      481 cttcaaatcg cgttttggat agagcttgtt atactctcgt attcgatcga agatgaccac
      541 tgtatcatta atggaatatc cgattatagt aagaatggcc gctatgaacg attggtctac
      601 ttccagattg aaaggcaaga taccatggaa gagcgagaat agtccgatga tgatgatggt
      661 atcgtgaaaa agactgatta cacctcccag accgaattgc catttattga agcgggcagc
      721 gatatagatg aatatgccca gtaaggcaaa aattaccgcc atgatggcat ttcgtttgat
      781 atcgtaagct atagttggcc ccactttttg tgaagtaagt attccaatga gtttaccgct
      841 ctgattaagg tcggaggcaa aatgagaaaa gttgatctca ttggcataca aacccctaag
      901 agcattataa attttacgat cgatcaatga atccacctcg cgtccttctt catcgattaa
      961 atacttggta gtaatcttca cctggttgct gggtccatag gtttttactt caggtgcctc
     1021 gccaaattca ttcgcaagca gttctcttat ttcgggcaca tttacatcac ggtcgaaacg
     1081 gactacataa gtacgacctc cggtaaaatc t
//
LOCUS       DAORPD010000731         1106 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_28172_length_1106_cov_1.998097, whole genome shotgun sequence.
ACCESSION   DAORPD010000731 DAORPD010000000
VERSION     DAORPD010000731.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28172_length_1106_cov_1.998097"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            686..>1106
                     /locus_tag="PK976_08165"
     CDS             686..>1106
                     /locus_tag="PK976_08165"
                     /inference="COORDINATES: protein motif:HMM:NF025652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4372 domain-containing protein"
                     /protein_id="HRC67902.1"
                     /translation="MNRFSSIFGHILHLFPRREFFEAVEATRADRDAKGFSCWDQFVA
                     MMFCQLGQAHSLREICGSLASCFGKARHLGIQTVPKRSTLAYANEHRPWQLYEKLFYQ
                     ILGRCRQLEFGKRKFRFKNRLFSLDASIIPLCASLFDW"
BASE COUNT          271 a          251 c          270 g          314 t
ORIGIN      
        1 gcactcgagg taaagtagac gtaattttgc gtcgacatct tttccaagaa gcttccatct
       61 ctggtatcta tcaaaaatgt cggatcccgt aatcactgca cttcttaaaa tagagacaat
      121 gcttgtgaag ggcatgggtg tatctcctac gcggccaacc ttaatctttg caatatgcgt
      181 gccctccgaa tgtgatttag agaagcataa gaagagatcg atttattatg cttcgaagaa
      241 tatttgccaa tgcactgctt aattgtcgct ttgatgattg tttgttccgt gcgagatggt
      301 gatttactac cgggctatct tgatagtttc gagaacgatc gacgattaat ataatgtatt
      361 ttcaactgct aatttgtgga ttggcatctt ttggagcact cgcgctccgc agggcgccat
      421 gtcatgacac caacatactc tggtaacgaa ttgtgaatta aaattttgca tattgcacgc
      481 acggtgattg tgaggttaaa atactgtgag caaaaaagta gtgaagaatg aagcacacat
      541 tgctttgtga ctttctttct gtatttttat tctttatact tgaacacatc tgtccaaaat
      601 aggtttttgt aattgaaaag ggtgcacacg atcaggtagt agtttctctg cgagaagaaa
      661 ttcaatcctg aaaggcggca ccctcatgaa cagatttagc agcattttcg gtcatattct
      721 acacctattt ccgcgacgcg agttctttga agccgtggag gccacccgag cggatcgaga
      781 cgccaaaggg ttcagttgct gggaccagtt cgtggccatg atgttctgcc agctggggca
      841 ggcccattcc cttcgggaga tctgcgggag cctggccagt tgtttcggca aggccaggca
      901 tttgggcatt caaacggtcc cgaaacgctc gaccctggcc tacgcgaacg agcatcgtcc
      961 ctggcagctc tacgagaagt tgttttatca gatcctgggc cgctgccggc aactggagtt
     1021 cggcaaacgg aagttccggt tcaagaaccg gctcttcagc ctcgatgcct cgatcatccc
     1081 gctgtgcgcc tctctgtttg actggg
//
LOCUS       DAORPD010000732         1105 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_28217_length_1105_cov_1.980952, whole genome shotgun sequence.
ACCESSION   DAORPD010000732 DAORPD010000000
VERSION     DAORPD010000732.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28217_length_1105_cov_1.980952"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="PK976_08170"
     CDS             complement(<1..423)
                     /locus_tag="PK976_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67903.1"
                     /translation="MDRTAHLFCLLIMISIAACKTPSSPDEKEYYDQILEKQNTLADA
                     IFDLNRYLEHADTAGLQRAYNKTLDYAKNSLQEIEKIGDYQGDTILLKATLSLLKVYC
                     EVLENEIQEMIAIVKKPGEISDADDARIMRLRKNIYKKL"
     gene            complement(416..877)
                     /locus_tag="PK976_08175"
     CDS             complement(416..877)
                     /locus_tag="PK976_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HRC67904.1"
                     /translation="MKIQNQEIRYQHIYGQLETLLTKTKVPEARMSTIAALLHHKMPN
                     FFWTGFYHLVDDELIVWCYQGPLACQILEKNKGVCWAAIQQQKTIIVPDVEQFPGHIA
                     CDSRSRSEIAVPLKDAGGNIKGVLDIDSQLIDNFNETDAKWLEKIIDMVYG"
BASE COUNT          318 a          215 c          196 g          376 t
ORIGIN      
        1 aagtttttta tatatgtttt tcctcaatcg catgatacga gcatcatcag catccgaaat
       61 ctcgcctggt tttttaacaa tagcaatcat ttcctgaatt tcattttcca atacttcaca
      121 ataaaccttc aaaagggaaa gagttgcctt taaaagaatt gtatcgccct gataatcacc
      181 aattttttct atttcctgta gagaattttt cgcataatcg agagttttat tataggctcg
      241 ttggaggccg gcagtatcgg cgtgttcgag ataacggttg agatcgaaaa tggcatcggc
      301 caaagtgttt tgtttttcta atatttgatc ataatattct ttttcatcgg gcgatgatgg
      361 agttttacat gccgcaatac ttatcattat gagcaagcaa aataggtggg cagttctatc
      421 catagaccat atctataatt ttttcgagcc atttggcatc ggtttcgtta aagttgtcaa
      481 taagctggct atcaatgtcc aatactccct tgatgtttcc ccctgcatct ttaaggggga
      541 cagcaatttc ggatctcgaa cggctatcac aggcaatatg accgggaaat tgctctacgt
      601 caggtacaat gatagttttt tgctgctgga tggcagccca gcatactccc ttgttttttt
      661 cgagtatctg acatgccagt ggcccctgat aacaccatac aatcaattca tcatctacaa
      721 gatgataaaa tcctgtccag aaaaaattcg gcatcttatg atgcagtaaa gctgctattg
      781 tgctcattcg agcctcagga accttcgtct tggtcaataa agtttctaat tgaccataga
      841 tgtgttgata acgtatttct tgattctgaa ttttcatcac aaatcaaata atgatttcaa
      901 ttacaatagt ttaactttta aacttcatca ctggcttcca aaattaccga aaatttggaa
      961 atctctcaaa aatttatatt atgaatttat tcgaaattca tcgatttaca aatcgtaagg
     1021 cttctttttt gcttaaaggc tttaagggtt ggtgagctac aaaatgcatc acttcttcgg
     1081 gaaatgtttt cgacacttgg cgtaa
//
LOCUS       DAORPD010000733         1103 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_28300_length_1103_cov_2.326336, whole genome shotgun sequence.
ACCESSION   DAORPD010000733 DAORPD010000000
VERSION     DAORPD010000733.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28300_length_1103_cov_2.326336"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="PK976_08180"
     CDS             complement(<1..695)
                     /locus_tag="PK976_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67905.1"
                     /translation="MQKRKTIIFLILISSIFTFQKAEGQVNIADSMIRIFGVSARLGS
                     HLPAADMAERFGISYTVGPAFTYKTNKNWIWDAGFDYIFGNDVKIKDSLFRLISTSHQ
                     FIIDQEGVPADVAVQERGYHFYVSSGKLFPIWGPNPNSGLIVEFGMGYLLHKVNISVL
                     NNSAPQLRGDYKKGYDRLTGGFSLNQFIGYQHLSSKRIYNFFVGLELIEGFTSSLREY
                     DFDRMAPAEGNRF"
BASE COUNT          361 a          217 c          209 g          316 t
ORIGIN      
        1 tcgaagcggt ttccctcggc aggagccata cggtcgaaat cgtattcgcg aagcgaagag
       61 gtaaatcctt cgattaattc gagacctaca aaaaaattat aaatcctttt cgaacttaga
      121 tgttgatatc caatgaactg attcaacgaa aaaccaccgg tcaatctatc ataacctttt
      181 ttataatcac ctctcagttg aggtgccgaa ttattgagaa ccgaaatatt gaccttatga
      241 agaagatagc ccatcccaaa ttccacaatc aaacccgaat taggattagg cccccatata
      301 gggaataatt ttcccgagga gacataaaaa tgataccctc tttcctggac tgcaacatcg
      361 gccggcacgc cttcctggtc gatgataaat tgatgcgagg tcgaaataag tcgaaatagc
      421 gaatccttta tcttgacgtc gtttccgaaa atataatcga agccagcatc ccatatccag
      481 tttttgtttg ttttataagt aaatgcaggc cctaccgtat agctgattcc aaaacgctca
      541 gccatatcgg cagctggaag atgacttccc aaacgggccg aaactccaaa gattcgaatc
      601 atagaatcag caatatttac ttgtccttca gctttttgaa aagtaaaaat cgagctgatg
      661 agaattaaaa aaataatagt tttccttttt tgcattcagt cttctttttt tctgtgaaga
      721 ttaaaataag cagcctatgt cgaatgaaac gcaaaagtaa gtaaaaacgt atctaatgca
      781 cgagaatgat ttattggttg aaattttaat tttattatat atcattcttg acatcgaatt
      841 cctgaatccg atcgaatggg gcaggaataa atcgagtgta tcggatagca gtaccggttc
      901 aaatgataat gcatatatat tctattggct tcgcgctggg ttgtataaat tctctacctt
      961 tgcgcaatgc aatgtaaaaa caattagaaa ttcatgagaa agatacaaat ggtggattta
     1021 tactctcagt atgtgaagat caaaccggag attgaccagg ccattcagga ggtaatagat
     1081 tccacggcgt ttattaaggg ccc
//
LOCUS       DAORPD010000734         1102 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_28356_length_1102_cov_2.155683, whole genome shotgun sequence.
ACCESSION   DAORPD010000734 DAORPD010000000
VERSION     DAORPD010000734.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28356_length_1102_cov_2.155683"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1102)
                     /locus_tag="PK976_08185"
     CDS             complement(<1..>1102)
                     /locus_tag="PK976_08185"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HRC67906.1"
                     /translation="ASLNYATFYSPVDGPYIETYFSVNGNSVVFAWNENGLFRATIEI
                     AMIFRQDEKIVNFKKYELLSPEIRDTTSVAVDFIDQQRIPLPVGKYDFELSITDKNNR
                     KESYVHHIPIEIYYSDTSLVISGIQLVESYNKSEKESILNKNGYELVPKVSTFFSSDQ
                     NKLIFYAEIYNAVEIFGEGQKFLVRTYISNYESGFHFNQFSKVKRMDAENVNVVFNEF
                     NITRLPSGNFNLVIEARDRQNRLVSINTLFFQRSNPGYIPDLTDIDSLSLRKSFASHI
                     KNPDTLREYIKCLRPIAADYEKFFIDKGMKDAPVEVMQSYLYSFWKNRSETDPEGAFK
                     AYLEEVAKVQTLFGTSYLRGYNTDRGRIFLQYG"
BASE COUNT          349 a          218 c          198 g          337 t
ORIGIN      
        1 ccatactgta agaaaatacg accacgatcg gtattatatc ctcttagata agaagtgcca
       61 aataaagtct ggaccttggc tacttcttcc agataggcct taaatgcacc ttcaggatcg
      121 gtttccgaac gattcttcca gaaagaatac aaataagact gcatcacttc cacgggtgca
      181 tctttcattc ccttgtcgat gaaaaatttt tcataatcgg cagcaatggg gcgcaggcat
      241 ttaatatatt ctcttagggt atcagggttc ttaatatgcg aggcaaagga tttacgcaac
      301 gataaactgt caatatctgt taagtcggga atataacccg gattgctcct ttgaaagaat
      361 aaggtgttaa tactgaccaa acgattttgc cgatctcggg cttcgatcac taagttgaaa
      421 tttccgctgg gcaaacgagt aatattaaat tcattgaaca ctacattgac attttcggca
      481 tccattctct ttaccttact aaactgattg aaatgaaatc ccgactcata attggagata
      541 taagttcgca cgagaaattt ctggccttca ccaaaaattt ccaccgcatt gtaaatctcg
      601 gcataaaaaa tcagtttgtt ctgatcggaa gagaaaaatg tacttacctt aggaacaagc
      661 tcatatccat tcttattaag aatcgactct ttttcacttt tattgtatga ttcaactaac
      721 tggattccag aaataaccaa gctggtatcg ctgtaataaa tttctatagg aatatgatga
      781 acataagact cttttctatt attcttatcg gtaatgctca gttcaaaatc gtacttaccc
      841 acgggaaggg gtatgcgctg ctgatcgata aagtcaactg ctactgaagt ggtatcgcgt
      901 atttcagggc taagtagttc atatttcttg aaattaacta ttttttcatc ttgtctgaaa
      961 atcatagcta tttcgatagt agcccgaaac aatccgtttt cattccaagc aaaaactaca
     1021 gaatttccat tgactgaaaa ataggtttca atataagggc catccactgg agaatagaag
     1081 gtagcataat tgaggctagc ct
//
LOCUS       DAORPD010000735         1101 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_28402_length_1101_cov_2.410134, whole genome shotgun sequence.
ACCESSION   DAORPD010000735 DAORPD010000000
VERSION     DAORPD010000735.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28402_length_1101_cov_2.410134"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /gene="leuD"
                     /locus_tag="PK976_08190"
     CDS             complement(<1..500)
                     /gene="leuD"
                     /locus_tag="PK976_08190"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HRC67907.1"
                     /translation="MPGVKFTTLESTCVPLPVENIDTDQIIPARYLSATSREGFGEKL
                     FYDWRYDRDGKPVMSFVLNNPLYSGSILVAGRNFGCGSSREHAVWALYDYGFRVVISS
                     FFADIFKNNALNNGLLPIVIPDEDLENLFMLIQKDPGMKVKITLEHQLLEIPKIGKAI
                     RFDINP"
     gene            complement(520..>1101)
                     /locus_tag="PK976_08195"
     CDS             complement(520..>1101)
                     /locus_tag="PK976_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HRC67908.1"
                     /translation="NFDREVEFDGGEIKPMITFGTNPGMAISVNAIIPEPPENEKEAI
                     SFMKALEYMGFKPGMKLEGHKIDYVFLGSCTNGRIEDFRAFASIIKGRKKSPDVTALI
                     VPGSKRVEAQIFEEGLDKIYREAGFEIRQPGCSSCLAMNDDKIPAGKYSVSTSNRNFE
                     GRQGPGARTILASPLTAAACAITGEITDPRNFL"
BASE COUNT          276 a          222 c          235 g          368 t
ORIGIN      
        1 taaggattta tgtcgaacct gattgccttt ccgatttttg gtatctcgag gagttgatgt
       61 tcaagagtga tttttacttt catgccggga tctttctgga tgagcataaa gagattttcg
      121 agatcttcgt ctggaatgac aattggcaga agtccgttgt tgagggcatt gtttttgaag
      181 atgtcggcaa aaaaactcga tattactact ctgaagccgt agtcgtataa tgcccatacg
      241 gcatgttcac gacttgagcc gcatccgaaa ttccttcctg ccacaagaat ggaacctgaa
      301 taaagagggt tattaaggac gaagctcatt acaggttttc catctctgtc gtagcgccag
      361 tcgtagaaaa gcttttctcc gaagccttct ctggatgtgg cactcagata tcttgccgga
      421 attatctggt ctgtatcaat gttttcaacc ggaagaggca cgcatgttga ttcgagtgtt
      481 gtaaatttta ctcctggcat tgttttctga ttaaaattgt tataagaaat ttcgtggatc
      541 ggttatttca ccggttatgg cgcatgcggc tgcagtaaga gggcttgcaa gtattgttct
      601 ggcgccgggg ccctgcctgc cctcaaaatt tctgtttgaa gttgaaacag aatattttcc
      661 tgcaggtatt ttatcatcgt tcatcgcaag gcatgatgaa catccgggtt ggcgtatttc
      721 aaaaccggct tctctgtata ttttatcaag gccttcttcg aaaatttgag cttcaactct
      781 ttttgagccc gggactatca atgcagttac atcgggagat ttttttcttc ctttgataat
      841 cgaggcaaat gctctgaaat cctcaatacg tccattggta caactaccga ggaaaacata
      901 atctatctta tgaccttcga gtttcatgcc tggtttaaag cccatatatt caagagcttt
      961 catgaatgat attgcttctt tttcattttc gggaggttca gggataatag cattaactga
     1021 tattgccatt cctgggtttg ttccaaatgt aatcataggt tttatttcgc ctccgtcaaa
     1081 ttccacttcc ctatcaaaat t
//
LOCUS       DAORPD010000736         1100 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_28451_length_1100_cov_2.288038, whole genome shotgun sequence.
ACCESSION   DAORPD010000736 DAORPD010000000
VERSION     DAORPD010000736.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28451_length_1100_cov_2.288038"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="PK976_08200"
     CDS             <1..495
                     /locus_tag="PK976_08200"
                     /inference="COORDINATES: protein motif:HMM:NF015291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HRC67909.1"
                     /translation="DTIIFTRQNPYKFRVTGRTCHFINAFGEEVIAENVERAFEIACK
                     ECNAQLSEYTGAPVYFSQNRNGAHQWLVEFEKTPEDFECFVETFDKALQSVNSDYEAK
                     RFHDMVLSSPIIQQVPRGTFYQWMKNRNKLGGQNKVPRLANERNIIEEILEMLQQPTT
                     KTNH"
     gene            535..>1100
                     /locus_tag="PK976_08205"
     CDS             535..>1100
                     /locus_tag="PK976_08205"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007134449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HRC67910.1"
                     /translation="MHIAIAGNIGSGKSTLTALLAKHFQWKPHFENVDTNPYLMSFYE
                     DMQRWSFNLQIYFLNSRFRQIVEIRKSGENVVQDRTIYEDAYIFAPNLHDMGLMDTRD
                     FQNYQSLFELMISFIQPPDLLVYLRATVPTLVRQIQKRGRPYEESIRLDYLKGLNERY
                     EDWISQYKAGKLLIIDVDHTDFASNTEDL"
BASE COUNT          357 a          236 c          206 g          301 t
ORIGIN      
        1 gatactataa ttttcacccg tcagaatcca tacaaattta gagttacagg ccgcacctgc
       61 cattttatca atgccttcgg agaagaagta atagccgaaa atgttgaacg tgcctttgaa
      121 attgcttgta aagaatgtaa tgctcaattg agcgaatata cgggcgcacc ggtctatttt
      181 agccaaaata gaaatggcgc tcaccagtgg ctggtagaat tcgaaaaaac acccgaagat
      241 ttcgagtgct tcgtcgaaac tttcgataaa gcccttcaat cggtgaattc cgattacgag
      301 gctaagagat tccatgatat ggtgttatcc agtcctatca ttcagcaagt ccctcgagga
      361 acattctatc aatggatgaa aaatcgcaac aagttaggtg gacaaaacaa ggttcctcgc
      421 ttagccaacg agagaaatat catcgaagaa attctcgaaa tgcttcagca acctaccact
      481 aaaacaaacc attaactcat tctatagaat ttatcttatt aaaaatttcg ccttatgcac
      541 atagctattg caggaaatat cggttcggga aaaagtacac tcactgcatt gcttgcaaaa
      601 cattttcaat ggaaacctca tttcgaaaat gtagatacaa acccttattt gatgagtttt
      661 tatgaagata tgcagcgttg gtcattcaat ctgcaaatct attttctcaa cagccgcttt
      721 cgtcaaatcg tcgaaattag aaaatcgggt gaaaatgtgg ttcaggacag aacaatatac
      781 gaagatgcct acatttttgc ccccaatctg catgacatgg ggcttatgga taccagagac
      841 ttccaaaatt atcaaagcct cttcgaactg atgatttcat tcattcaacc ccctgacctt
      901 ttagtgtatt taagagctac cgttccgact cttgtgcgtc agattcagaa acgaggccgc
      961 ccttacgagg aaagcatacg cctggattac ctcaaaggat tgaatgaacg gtatgaagac
     1021 tggatttcac aatataaagc cggaaaactt ctcatcatag atgtagatca taccgatttt
     1081 gcctctaata ccgaagacct
//
LOCUS       DAORPD010000737         1099 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_28492_length_1099_cov_2.836207, whole genome shotgun sequence.
ACCESSION   DAORPD010000737 DAORPD010000000
VERSION     DAORPD010000737.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28492_length_1099_cov_2.836207"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1095
                     /locus_tag="PK976_08210"
     CDS             <1..1095
                     /locus_tag="PK976_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HRC67911.1"
                     /translation="LGASGDLAPLAHLSLPLLGLGEVTYKGEKMPASKALFEAGLSPV
                     DLKSKEGLALLNGTQFMGAYGVYCLLRSFRLSTLADLIGALSLDAFDGRIEPFYLPLH
                     EIRNHKGQISTAKGIANLLESSELMNKPKNHVQDPYSFRCIPQVHGASKDAIEYVAKV
                     FSCEMNAVTDNPTIFPYEDLIISGGNFHGQPLALALDFLAIALSELGNISERRTYQLI
                     SGKRNLPHFLVVNPGLHSGFMIPQYTAASIVNQNKQLCTPSSVDSIESSQGQEDHVSM
                     GANAATKALKVVNNLQRILAIELFNAAQALEFRRPAKSSPLIENFVHKYRQYVPFIQY
                     DEVMHPHIENSIRFLSEVKLPISDLFIPNL"
BASE COUNT          288 a          257 c          228 g          326 t
ORIGIN      
        1 ttgggagcgt cgggcgatct ggcgcctctg gctcatttgt cgctaccctt attaggattg
       61 ggtgaagtaa cttataaagg ggaaaaaatg cctgccagca aagccctgtt cgaagccggt
      121 ttgagtcctg ttgatttgaa atcgaaggaa gggctggctc ttttgaacgg tactcagttt
      181 atgggagctt atggagttta ttgtcttttg agaagttttc gcctttctac tcttgctgac
      241 ttgattgggg cactttcgct cgatgccttc gatggcagaa tagaaccttt ttatttgcct
      301 ttgcatgaaa ttagaaacca taaagggcag atttccactg caaaaggaat tgccaatctt
      361 ctcgagagca gtgagctgat gaacaaaccc aagaatcatg tgcaggatcc ttatagcttc
      421 cgttgtattc cgcaagtaca cggcgccagt aaagatgcca ttgaatatgt tgcaaaggtt
      481 ttttcgtgcg aaatgaatgc agttaccgat aaccctacca tttttcccta tgaagacctt
      541 atcatttcag ggggaaattt tcacggacaa cctcttgccc ttgcgctcga ttttttagct
      601 attgcccttt ccgaattagg aaatatctcg gaacgtcgca cttatcagct catttcaggg
      661 aaacgaaatc taccgcactt ccttgtagta aatcctggcc tgcattctgg ctttatgatt
      721 ccacaatata ccgctgcaag catagtcaat cagaataaac agctttgcac tccttcttcg
      781 gtcgattcca tcgaaagttc gcaaggtcag gaagaccatg taagcatggg ggctaatgca
      841 gccaccaagg ctttgaaagt ggtaaataat ttgcagcgca ttctggccat cgaactcttc
      901 aatgcagcac aagcactcga atttcgccgt cctgcaaaaa gttctcccct catcgaaaac
      961 tttgtccata aataccgcca gtatgttcct ttcattcaat atgatgaagt gatgcatccc
     1021 catatcgaaa attcgatacg ttttctttct gaagtaaagc tcccaatttc tgaccttttc
     1081 atccccaacc tttaaatcc
//
LOCUS       DAORPD010000738         1099 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_28498_length_1099_cov_2.268199, whole genome shotgun sequence.
ACCESSION   DAORPD010000738 DAORPD010000000
VERSION     DAORPD010000738.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28498_length_1099_cov_2.268199"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="PK976_08215"
     CDS             complement(<1..213)
                     /locus_tag="PK976_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67912.1"
                     /translation="MSRKQKKRKIARHLEIMGIHPSGKGWTLFQGRKVVIPNTITGEI
                     ISGEIVYGGSEFLSGILIEIEKQHPYR"
     gene            complement(311..604)
                     /locus_tag="PK976_08220"
     CDS             complement(311..604)
                     /locus_tag="PK976_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67913.1"
                     /translation="MKAVFIVFNQAITDKVENALELAQIKGFSKWNEMAGSGSNEGEP
                     HLGSHTWPALNSGILIVLEDEKVKTLLNLVEQINKIAETQGIHAFVWNIETMV"
     gene            complement(674..>1099)
                     /locus_tag="PK976_08225"
     CDS             complement(674..>1099)
                     /locus_tag="PK976_08225"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRC67914.1"
                     /translation="TGVLIALAITNTSINIISGIGIVMLVGIAVKNSIVLVDFINLLV
                     SRGYSIKDAIIAGGRSRLRPILMTSFTTLLGMLPLGISQGEGSEIWRPLGISIIGGLF
                     FSMLISLIIVPVIYSLFAHAKKRREENNKAAQELFLPSE"
BASE COUNT          330 a          217 c          195 g          357 t
ORIGIN      
        1 cctgtagggg tgctgttttt ctatttctat tagaattccg cttaaaaatt ccgaacctcc
       61 atatacaatc tctcccgaaa tgatttcgcc agttatggtg tttgggataa caaccttcct
      121 cccttgaaat aatgtccaac cttttcctga aggatgtatg cccattattt ccaaatggcg
      181 tgcaattttt cgtttcttct gtttacggct catggaattt caaaaatata acaaaccacc
      241 ttagcatgta tagctatagg tggtttgtta gaatgttgat aaagtttagt ttgatttgtt
      301 aactaaacca ctaaaccata gtttctatat tccagacgaa ggcatgtata ccttgagttt
      361 ctgctatttt attgatttgt tcgactaaat tgagcagagt tttaactttt tcgtcttcta
      421 aaacgataag aatacctgaa tttagggccg gccaagtatg agaacccaag tgaggctcac
      481 cttcattaga acccgagcca gccatttcgt tccatttact aaagccttta atttgagcta
      541 attctaaggc attctccact ttatcggtaa ttgcctgatt aaaaacgata aatactgctt
      601 tcattttttt tattttttaa tgagaaaagg tgtcagtgct cgaaaaataa gcaccaacac
      661 ctgaaattag actttattcg gaagggagga ataattcctg ggcggcttta ttgttttctt
      721 ctcttctctt tttcgcatga gcaaataaac tgtaaattac tggcacgatg atcagagaga
      781 taagcataga gaaaaacaat cctccgataa tagaaattcc taatggacgc cagatttcag
      841 aaccctcacc ttgagaaata ccgagaggaa gcatacccag aagggtggta aatgaagtca
      901 ttagaatagg tcttaacctt gatcttccac cagctataat ggcgtctttt atggaatatc
      961 ctctgctaac gagcaaattg atgaaatcga ccaatacgat ggagtttttc actgcaattc
     1021 cgactagcat tactatccca atacccgaaa tgatattgat actggtgttt gtaatggcca
     1081 atgcgattaa tacacctgt
//
LOCUS       DAORPD010000739         1099 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_28500_length_1099_cov_2.203065, whole genome shotgun sequence.
ACCESSION   DAORPD010000739 DAORPD010000000
VERSION     DAORPD010000739.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28500_length_1099_cov_2.203065"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="PK976_08230"
     CDS             <1..807
                     /locus_tag="PK976_08230"
                     /inference="COORDINATES: protein motif:HMM:NF023280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3372 domain-containing protein"
                     /protein_id="HRC67915.1"
                     /translation="QMPRIAVFSDELRDGVKGPWYNSTESGFIGGVTGLEESIKFGIV
                     AATKHPQIDYQKVNYSKAPYAAQPDQCINYVSCHDNHTLWDKIRLTVKNASEEELVKM
                     NMTASAIVLTSQGIPFLHNGVEMMRSKKGVENSFNSPDSINQIDWNWKYLYSRAVKFH
                     QQLIELRKQHPAFRMKTSNEISENLHFLSIPKPCIIAYTLNGKAVGDSWRKILVIFNG
                     NRHPENITIPDEHWKIIVNGDRFLPTPVPYTEKGHLTLPPSSVIILAVTE"
     gene            824..>1099
                     /locus_tag="PK976_08235"
     CDS             824..>1099
                     /locus_tag="PK976_08235"
                     /inference="COORDINATES: protein motif:HMM:NF014919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan-branching enzyme"
                     /protein_id="HRC67916.1"
                     /translation="MEYKKLKIVKEDPWLEPVEKEVFDRYQRYKNRLNEITSQWGSLL
                     KFANAHNYLGIHYSKKDKGWYYREWAPRAFDLYLMGDFNNWDRFSHHL"
BASE COUNT          364 a          220 c          217 g          298 t
ORIGIN      
        1 caaatgccaa ggatagcggt tttttcggat gaattgcgtg atggtgtaaa aggaccatgg
       61 tacaatagta cggaatccgg ttttatagga ggagtaaccg gactggaaga aagtatcaag
      121 tttggtattg tggcggctac aaaacacccc caaatagact atcagaaggt aaattattcc
      181 aaagctcctt atgcggctca acccgatcag tgtatcaatt atgtatcttg ccacgataat
      241 catacactct gggataaaat acgcttaacg gtaaaaaatg cttccgaaga agaactcgta
      301 aaaatgaaca tgactgccag tgccatagtg ctcacttcac agggaatacc ctttttgcat
      361 aatggcgtag aaatgatgcg ttcgaaaaaa ggagttgaaa actcatttaa ttcgcccgat
      421 agcatcaacc aaatcgactg gaactggaaa tatctttaca gccgtgcagt aaaattccat
      481 caacagctca tcgaacttag aaaacagcat cctgcattta gaatgaaaac ctcgaatgaa
      541 atctcggaaa atctccattt cttgtccatt ccgaaaccat gcataatagc ttataccttg
      601 aatggaaaag cagtaggcga tagttggcgt aaaattctgg tcatcttcaa tggaaatcgc
      661 catcccgaaa atattaccat acccgatgaa cattggaaaa taatcgtaaa tggcgataga
      721 tttttaccta ctccagttcc ttataccgaa aaaggtcatc ttactttacc cccatcttct
      781 gtcattatac ttgcagttac tgaataatac tctttatttc tctatggaat acaaaaaatt
      841 aaagatagtc aaagaagacc cttggttgga gccagtcgaa aaagaggttt ttgatcgtta
      901 tcaacgttat aaaaatcgtt tgaacgaaat aaccagccag tggggtagcc ttctgaaatt
      961 tgccaatgca cacaattatc tgggcattca ctacagcaaa aaggacaagg ggtggtatta
     1021 ccgggaatgg gctccacgtg cattcgatct ttatttaatg ggcgacttca ataattggga
     1081 caggttttcg catcatttg
//
LOCUS       DAORPD010000740         1091 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_28844_length_1091_cov_2.269305, whole genome shotgun sequence.
ACCESSION   DAORPD010000740 DAORPD010000000
VERSION     DAORPD010000740.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28844_length_1091_cov_2.269305"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            445..>1091
                     /locus_tag="PK976_08240"
     CDS             445..>1091
                     /locus_tag="PK976_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008774254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylase"
                     /protein_id="HRC67917.1"
                     /translation="MTKRRILIRDLTLRDGQQSLFATRMTQEQIDELLPLFKEAGFYA
                     MEVWGGAVPDSIMRYLKEDPWERLEKISKAIGNSSKLTALSRGRNLFGYNPYPDEVIE
                     GFLRNAVESGIRIMRVFDALNDTNNIESSIRFLKKYGATIDAALCYTVDPKFSTRARV
                     KAFLHGKSLPNRIFTDDYFVNLARELEAMGADMLTIKDMAGLITPMRTYRLISRL"
BASE COUNT          345 a          188 c          206 g          352 t
ORIGIN      
        1 aaaaaaatcc catacttcct acatggatgg taatccataa tgcaagctta tcaaggactg
       61 ataatttttc cctgtattgc tcattgacat tatgaatcgg atgatactgc cttttgattt
      121 cctcgagaga tttgatttcg tgatgtttca ttttcaatca ttttttaatg aaaacaatat
      181 tataaagatg taactcgttt ttcgataaag ggagaaaagt gatttagggc tatgaaatta
      241 taactttttc acgctttgcg aatatgcacc tttagaattt tgattaaaac cttattgaca
      301 ttataatatg cagataatca aaatttattc atcaatcaaa taacaatcga ccattgcgtt
      361 ccggaattcc ctttaccttt gaagacagaa atagatagtg atttttattt tttaagctat
      421 tattaatcag caacataata ttttatgacg aagagaagaa ttttaatcag ggatttaacc
      481 ttacgcgatg gacaacagtc attatttgct acgcggatga cgcaagaaca aatagatgag
      541 ttacttccgt tgttcaagga agcaggtttc tatgccatgg aagtatgggg aggtgctgtt
      601 cctgattcaa ttatgcgtta tttgaaagaa gatccatggg agagactcga aaaaataagt
      661 aaggcgatcg gaaattcttc taagctcact gctttgtcga gaggaagaaa tctcttcggt
      721 tataatcctt atcccgatga agtcattgag ggatttttac gaaatgctgt cgaatcggga
      781 attcgaatca tgagggtttt cgatgccctg aatgacacga ataatattga atcatccatt
      841 cgatttttaa aaaaatatgg tgcaacgata gatgccgctc tatgttatac ggtagatccc
      901 aaattctcga cacgtgcacg tgttaaagct tttttacatg gcaaatcttt acccaatcgc
      961 atctttaccg acgattattt tgtcaacctg gccagggaac tcgaagctat gggtgccgac
     1021 atgcttacca taaaagatat ggcaggctta atcactccta tgagaactta tcgtctgatt
     1081 agccgattga a
//
LOCUS       DAORPD010000741         1088 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_28992_length_1088_cov_2.058083, whole genome shotgun sequence.
ACCESSION   DAORPD010000741 DAORPD010000000
VERSION     DAORPD010000741.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28992_length_1088_cov_2.058083"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1088
                     /locus_tag="PK976_08245"
     CDS             <1..>1088
                     /locus_tag="PK976_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HRC67918.1"
                     /translation="DVQWLKENIDFTGEIFLGHLRYGTYGKNSIQNLHPFLRENNWKT
                     RNLVLAGNFNLTNVDELFKTLVDLGQYPVETSDTVTVLERIGHFVDEENERLFKAFRA
                     KGYSKKEITDFIITHFDLKSVLTRAASKWDGGYVMAGIIGHGDAFIMRDPAGIRPAYF
                     YADDEVVIATSERPVIQTTLNVKAHQIDEIPPAHFLQVKSNGEWSLQQFAEPIRTASC
                     SFERIYFSRGTDQEIYHERKQLGKMLLPSILKAIDYDLENTVFSYIPNTAAVAFQGLM
                     EALAEFCDEKRKEKLLALDYTPTKNEIDEIFNIKPRFERIAVKDIKIRTFIAEDEQRD
                     DLVAHVYDVTYGAIRAGIDNLVVLDDSI"
BASE COUNT          351 a          199 c          236 g          302 t
ORIGIN      
        1 tgatgtgcag tggctgaagg aaaatattga ttttaccgga gaaattttcc tgggacattt
       61 gcgctatggt acatacggaa aaaattccat acagaatctt catccgtttt tacgggaaaa
      121 taactggaaa accagaaatc ttgttttagc gggcaatttc aacctcacca acgtagatga
      181 acttttcaag actttagtcg atttggggca atatcctgtc gagacttctg acacggttac
      241 tgttctcgaa agaattggac attttgtgga tgaagaaaat gaaaggttat tcaaagcatt
      301 tagagctaaa ggctattcaa agaaagagat taccgacttt atcatcactc atttcgattt
      361 gaagagtgtt ttaaccagag cagcttcgaa atgggatgga ggctatgtaa tggccggcat
      421 aataggtcat ggagatgcct ttattatgcg tgacccggca ggaatacggc cggcctactt
      481 ttatgccgat gatgaagtag taatagcaac ttccgaaagg cctgtcatac aaaccactct
      541 gaatgtcaaa gcccatcaga tagatgaaat accaccagct cattttttgc aggtaaaaag
      601 caatggagaa tggtcattac agcaatttgc tgaaccgata cgaactgcct catgctcatt
      661 cgaacgaatt tatttttcga gaggaaccga ccaagaaata tatcacgaaa ggaaacaatt
      721 gggtaaaatg ctcctgcctt ctattctgaa ggcaatagat tatgatctgg aaaatacagt
      781 tttttcatat atacccaata ctgccgctgt tgcatttcag ggtttaatgg aagcccttgc
      841 cgaattttgt gatgaaaaac ggaaggaaaa attactggca ctcgattata ctcccactaa
      901 gaatgaaata gatgagattt tcaatatcaa gcctcgattc gaaagaattg cagtaaagga
      961 tatcaaaatc aggaccttta ttgccgaaga cgaacaaagg gatgatctgg tagctcatgt
     1021 ttacgatgtg acttatggag ctatacgtgc aggaatcgac aatctggtag ttctggacga
     1081 ttccattg
//
LOCUS       DAORPD010000742         1086 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_29077_length_1086_cov_1.956353, whole genome shotgun sequence.
ACCESSION   DAORPD010000742 DAORPD010000000
VERSION     DAORPD010000742.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29077_length_1086_cov_1.956353"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..298)
                     /locus_tag="PK976_08250"
     CDS             complement(74..298)
                     /locus_tag="PK976_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67919.1"
                     /translation="MQKCITSVLQEMESALEMCGIRITSYVSNISSKSIQKIIDSIVD
                     GSDDMDQLKELAHSRIGNKHRERKEEMELL"
BASE COUNT          299 a          230 c          172 g          385 t
ORIGIN      
        1 cgaatttact cattctgcct ccatttcggc gatgatactc atggaagatg atttttccca
       61 tcgcacggat ggtttacaat agttccatct cttcttttcg ttccctatgc ttattgccaa
      121 tccgactgtg tgccaattct ttcaattgat ccatatcatc actcccgtca acaatactgt
      181 caattatctt ttgaatactt ttcgacgata tattgcttac atagctggta atacgaatgc
      241 cacacatttc caaggcagat tccatttcct gcagaacact tgtgatacac ttttgcaata
      301 tgccttattt tcatgtataa gttcgcaatt ccctgatatg cttatccgga accaaacttg
      361 ggttaaaaca tacctctctg taaaaacacg acaatccatt gcacatcttt tacatcgctc
      421 gttctatctg gcatttgttt tatccgatat ggattcacta aaaagcaact caaatcccat
      481 ttcttcgaat aggttcccta gtggtatccc ataaattcat ggactctcca tggcaattct
      541 gttcgctttc atctctctca aatacttgcc catagaatgc cacaccttag ggtcgaaaat
      601 ttcttcactt caaaagtgtt cttcatgttg tttattcctc aaaatattgt aattttgtac
      661 aagtcaagac cgactgctat cacatgtact ttccgattaa aagttaaaca tgaagtcgac
      721 caattgagat atgcctttaa ggctaagctc gttcgaactt acacattagc agcggtcttg
      781 attctttcat attcttattc gtcaacatat tcatgggtaa ttatcttatc aattcgtaaa
      841 actttctgta tgattatccg aatagacaga ggttcgctcg agtgtattag attttggctg
      901 tttcgtttta tttcattcga agatggtcaa atttgattgg aatcgatagt gtatgtttgt
      961 gggtttgact aaaaattcga tcgagacaga tgtacgaaca taaaaaccat aaattgagca
     1021 aatatctgaa agtggctggc ctcatcacag gaatcgttgg tttattattt ttcctggtat
     1081 tttatt
//
LOCUS       DAORPD010000743         1085 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_29112_length_1085_cov_2.666019, whole genome shotgun sequence.
ACCESSION   DAORPD010000743 DAORPD010000000
VERSION     DAORPD010000743.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29112_length_1085_cov_2.666019"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..>1085
                     /locus_tag="PK976_08255"
     CDS             131..>1085
                     /locus_tag="PK976_08255"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HRC67920.1"
                     /translation="MPLKQDIPIFFIIGRPRSGTTLIRTLLDAHPNVIVPPEYPVIPD
                     LIHRFGLYPRWTPKLKNELLQAFRQPQTFEFWNYDYLQIDEQSLMKDLDEMQENTPIT
                     SVIKLFYLHSKSVFPKKEIQLIGDKNPVYALYTRLLMKWFPEAKFLFIMRDPRDNFVS
                     MKKFDWEAPNPSIQAWRWKYITKMMFELNRKYPERTFLFLYEDLVRKPQTLFKKICDF
                     LDIVYDKNVFEFYKISSHENLGFIERETFLKYHESLTKPINTETIGKWEKELQSDEIK
                     TIDYVAGQWAQKVGYSLKYTRKSIVLPIKTWPFEIYGWLLYK"
BASE COUNT          380 a          218 c          181 g          306 t
ORIGIN      
        1 taccaccgaa agagttacca ttacgaaata atcgtatctg tctttctaaa aaagggcctc
       61 caaaacgggg gctctttttt tataccattt agcttcccta actttgatag tgaaaatgaa
      121 caacccattt atgccactaa aacaagatat tcctattttt tttattattg gcaggccacg
      181 atcaggaact acattgataa ggaccttact ggatgctcat cctaatgtta ttgtacctcc
      241 cgaatatcca gtaatacctg atctgatcca tcgtttcggc ttatacccac gatggacacc
      301 aaaattgaag aatgaacttc tgcaagcttt caggcaacct caaacttttg aattctggaa
      361 ttacgattac ctccaaatag atgaacaaag cttgatgaaa gacctggatg aaatgcaaga
      421 aaacaccccc ataaccagtg taataaaatt attctatctg cacagtaaaa gtgttttccc
      481 caaaaaagag attcaattga ttggagacaa aaatcccgta tatgctctct acactcgcct
      541 actcatgaaa tggtttcccg aggcaaagtt tcttttcatt atgcgtgatc cacgcgataa
      601 tttcgtgtca atgaaaaaat tcgactggga agcgccaaac ccatcaattc aggcatggcg
      661 atggaaatat atcacaaaaa tgatgttcga actcaacaga aaatatccag aaagaacctt
      721 tctgtttctt tatgaagacc tggtcagaaa gcctcaaacc ctgttcaaaa aaatctgtga
      781 ttttctcgat atcgtatatg ataaaaacgt ttttgaattc tataaaataa gtagtcatga
      841 aaatttagga ttcatcgaaa gagaaacttt tctgaaatat catgaaagcc tcactaagcc
      901 aatcaataca gagactatag ggaaatggga aaaggaactc caatccgacg aaatcaaaac
      961 aatagattat gtcgctggtc aatgggctca aaaagtgggt tattccctaa aatatactcg
     1021 aaaatcaatt gttttgccaa taaagacatg gccctttgaa atttatggat ggctgctgta
     1081 taaat
//
LOCUS       DAORPD010000744         1084 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_29177_length_1084_cov_1.623907, whole genome shotgun sequence.
ACCESSION   DAORPD010000744 DAORPD010000000
VERSION     DAORPD010000744.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29177_length_1084_cov_1.623907"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="PK976_08260"
     CDS             <1..299
                     /locus_tag="PK976_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67921.1"
                     /translation="GYIQLKHQENAREDQLRILLRPDEDHYFYIDYGRGNVLRVYSTN
                     KEFTDLVVKERESFNKQMEKDNPAKNWQPFRVAPVNRAEAIDFRAEMEKVKLFN"
     gene            309..968
                     /gene="plsY"
                     /locus_tag="PK976_08265"
     CDS             309..968
                     /gene="plsY"
                     /locus_tag="PK976_08265"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HRC67922.1"
                     /translation="MLITIVLIIIAYSIGSFSSAVWIGKWFYGIDVRRYGSMNAGATN
                     TLRVLGWKAGIPVLLLDALKGWIAVKLAAWLMPKPLGELQQELLLIACGIAAVLGHIF
                     PLYSRFKGGKGVATLLGIGLALFPYPTLGAVVVFIIFMILSCIVSLSSILAGISFPFL
                     VVFLPYQPRPHWPFLVLSILVAIFIPFTHLSNIRRLLKGEEKKFSIAKNAPRKEINLE
                     K"
BASE COUNT          299 a          194 c          243 g          348 t
ORIGIN      
        1 cgggatacat tcagcttaag catcaggaga atgcccgtga agatcagttg aggatattgc
       61 tgagacccga tgaagatcat tatttttata ttgattatgg cagaggtaat gtgttgaggg
      121 tttacagtac gaataaggaa tttaccgacc ttgtggtgaa agaacgagaa agtttcaata
      181 aacaaatgga gaaggacaac ccggccaaga attggcagcc ctttcgtgtg gccccggtga
      241 atagagccga agccattgat tttagagccg aaatggaaaa ggtaaagcta ttcaattaat
      301 attttttaat gttgataacc attgttttaa ttattattgc ttattcaatt gggagttttt
      361 catcggcagt atggattggg aaatggtttt atggcattga tgtgcgccga tatggcagca
      421 tgaatgcagg agcaaccaat actttgcggg ttcttggatg gaaggcaggc attccggttt
      481 tgcttttgga tgcactgaag ggatggatag ctgtgaaatt agccgcatgg cttatgccta
      541 agcctttagg tgaactgcaa caggagctct tattgattgc ttgcggcatt gcggctgttt
      601 taggccatat cttcccatta tattcaagat ttaaaggggg caagggtgtg gctactttac
      661 tgggaatcgg cctcgcttta ttcccttatc ctactctggg cgccgttgtt gtctttatca
      721 tctttatgat cctttcctgc atcgtatccc tttcgtcaat tctggcagga atcagtttcc
      781 cattccttgt ggtttttctt ccttatcaac ctcgaccaca ctggcctttt ctcgtcctga
      841 gtattttagt ggctattttt atccctttca ctcatctatc caatatccga agacttctta
      901 aaggagaaga gaagaaattt tccatagcca aaaatgcacc tcgaaaagaa attaatctgg
      961 aaaaataaga atagaaagtc ttggtagttt tcgaattaaa aaatattgca tgactggaat
     1021 gacaataata tgcaagggtt taagttatta ttcacacgag tatgttaaca acatttctat
     1081 tgtt
//
LOCUS       DAORPD010000745         1081 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_29298_length_1081_cov_1.991228, whole genome shotgun sequence.
ACCESSION   DAORPD010000745 DAORPD010000000
VERSION     DAORPD010000745.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29298_length_1081_cov_1.991228"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..783)
                     /locus_tag="PK976_08270"
     CDS             complement(79..783)
                     /locus_tag="PK976_08270"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HRC67923.1"
                     /translation="MILIPFVILSNGQDLKPGTQIPRAGKGIFVKKAQAYPYLLFLPN
                     NFVADHQHSYPLLLFLHGSGERGNDLELVKKNGTPGFVESMEDFPFVMLTPQCPQGET
                     WDSERLMALVNEAIENYSIDTNRMYITGLSMGGYGTFDLACNYPGKFAAILPVCCGGQ
                     KLKAEKLIHTPIWAFHGADDTIVPVSESKNMVEIVNRLGGNAHITILPGVGHNAWEYV
                     YIRPEIYQWLLSHQLK"
BASE COUNT          311 a          197 c          228 g          345 t
ORIGIN      
        1 gtaggcgttt ttgtgcatgc aaaagcactg aatatcacga aaaatataag aaaattttta
       61 gttttcttca ttttaatctt attttaattg atgtgataat aaccattgat atatttctgg
      121 acgaatgtaa acgtattccc aagcattgtg gccaacacca ggtagaatgg taatatgtgc
      181 atttccacca agtctgttca ctatttccac catgtttttc gattcactca ccggaacgat
      241 agtatcgtca gcaccgtgaa atgcccagat gggtgtgtgt atgagttttt cggcttttaa
      301 tttttgacct ccacagcaca cgggcagtat agcagcaaat tttccgggat aattgcaggc
      361 aaggtcgaaa gttccataac cgcccatact taaaccagtt atatacattc tattggtatc
      421 gatagaatag ttttcgatcg cttcgttgac taatgccatc aggcgttcac tgtcccatgt
      481 ttctccttgg gggcattgtg gtgttagcat tacaaaagga aaatcttcca tggattcgac
      541 gaatcctggc gtgccatttt ttttgaccaa ctcgagatca ttgcctcttt cgcccgaacc
      601 gtgaagaaaa agtaagagag gataagaatg ttgatgatcg gcgacgaagt tattgggtaa
      661 aaatagaaga tatgggtagg cctgagcctt tttgacgaaa atgcctttac ccgctcgagg
      721 aatttgagtt ccgggtttca agtcttggcc attcgataga atgacaaaag ggattagaat
      781 cataagaaga aaggtgattt ttaccatatc atgcaaatat taactgtaaa atctatttcg
      841 tcggcattaa gatacgaccg ggcagttttg cttatccaat gaattgaatg tttagctatc
      901 cataccgtct gaaatttcgg agaattttca gttaagtttc agaatagttc gatcgaaaat
      961 ctgctccttg gattcgctgc attcgcggca tggtgtatat ggcatgtgat aaaaagttgt
     1021 tgatagaggg ttttatgtaa atttagttct cgaagtcaaa tatccatttc gtcccattat
     1081 g
//
LOCUS       DAORPD010000746         1079 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_29394_length_1079_cov_2.500000, whole genome shotgun sequence.
ACCESSION   DAORPD010000746 DAORPD010000000
VERSION     DAORPD010000746.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29394_length_1079_cov_2.500000"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1079
                     /locus_tag="PK976_08275"
     CDS             <1..>1079
                     /locus_tag="PK976_08275"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HRC67924.1"
                     /translation="IHQMMNDYLGIINYHVRPDPNGTYIDHIDCWAKFLDVDKILVRQ
                     VPSSHPQYNLIEQAAAYWQSAISSYGTPYQVFRVNTPNNQPYTNSFILKDHVFVPFTG
                     NTLYDNEALQAYQNAMPGYQIIGVYQNPSTPWESTDALHCRTHEVADKYMLFIKHLPI
                     VHTQPCDEDYLITTYIKSLCDSAIIADSTRVFYSIDNGPYQIIPLSYQSNDQYTALIP
                     RQPAGSSIRYFLQAAEKSGRNMYHPFIGVADPHNFMVGEGIHPHITLSTHSVDTSSNP
                     GQVVNFPISITNVGYADLAWILQIDSASSSWLYSSDIMGTLSPGSTQDITLTLDATFL
                     SPALYESKISIQSNDPDQPVDNILV"
BASE COUNT          296 a          276 c          185 g          322 t
ORIGIN      
        1 agattcatca aatgatgaac gattatctgg gaataatcaa ttatcacgtt cggcccgatc
       61 ctaatgggac ttacatcgac catatcgatt gctgggcaaa atttctcgac gtcgataaaa
      121 ttcttgtccg tcaggttcca tcttctcatc cacaatataa tctgatagag caagctgcag
      181 cctattggca atctgctata agcagttacg gcactccata tcaggtattt cgtgtaaaca
      241 caccgaacaa tcaaccatat accaactcgt ttatcctcaa ggatcatgtc ttcgttccct
      301 tcaccgggaa taccctgtat gataatgagg ccttgcaagc ataccaaaac gcaatgcccg
      361 gttatcagat cattggggtt tatcaaaatc cttctactcc atgggaatct accgatgctt
      421 tacattgccg cactcacgaa gttgccgata aatatatgct ttttataaaa catcttccta
      481 tcgtgcatac tcagccttgc gacgaagatt atcttatcac gacatatatt aaatctttgt
      541 gtgattccgc catcattgcc gattctaccc gggtattcta tagcatagat aatggtcctt
      601 atcagatcat acctctcagc tatcaatcca atgatcaata tacagcactt attccacggc
      661 agcctgccgg aagcagtatt cgttacttct tgcaagccgc tgagaaaagt ggccgtaata
      721 tgtatcaccc cttcattgga gtcgccgatc cccataattt tatggtaggc gaaggaattc
      781 atccacatat tacactttct acgcatagtg tagatacttc ctccaatccg ggtcaggtag
      841 taaatttccc tatttccata accaatgttg gttatgctga ccttgcctgg attttacaaa
      901 tcgattcggc ttcatcgtcc tggctctatt cttccgatat catggggaca ttatctcctg
      961 gatcaactca agatattacc ctgactctcg atgcaacttt cctcagtccc gcgctttatg
     1021 aaagcaagat atccatccaa tcgaacgacc ccgatcagcc tgtagataac attctcgtt
//
LOCUS       DAORPD010000747         1078 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_29440_length_1078_cov_2.556207, whole genome shotgun sequence.
ACCESSION   DAORPD010000747 DAORPD010000000
VERSION     DAORPD010000747.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29440_length_1078_cov_2.556207"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="PK976_08280"
     CDS             <1..374
                     /locus_tag="PK976_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67925.1"
                     /translation="IMITDMCFQLLGWDVIYAHILRTNDKAIKYNKSLGYVLCEDQED
                     KENQLYRLRPESFHRKTQKLRKAISNLYSEKDEAYLIIEPSDIANDILSQLEPFFQLV
                     RRQRGDFESYFNADGSITFTF"
     gene            complement(364..>1078)
                     /locus_tag="PK976_08285"
     CDS             complement(364..>1078)
                     /locus_tag="PK976_08285"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HRC67926.1"
                     /translation="VVSGKEIITHIGDKNPIFATHAFRLRKIFPKAKFIFIFRDYRDN
                     FLSVKKFRFEAPIIALQAYRWKYVARLAYQFEKKFPEQTLIIRYEDLTRKPREILSHT
                     CTFLGLPYDETMLDFWTYSSKYSEFVDPELLNQFHSGLQSPIQTSSSGKWMKEMKQKE
                     IKTADQVVGRYAERLGYQRVDKKFHPITWLKTLPWQLYGFILYRMMSLAEFLPASLRQ
                     KFSMTLPKLTNIYHKWFKK"
BASE COUNT          339 a          189 c          202 g          348 t
ORIGIN      
        1 ccatcatgat cacggatatg tgttttcagc ttttgggatg ggatgtgatt tatgctcata
       61 ttttaaggac aaatgataag gccataaagt acaataagag tctgggatat gttttgtgtg
      121 aagatcagga agataaagaa aatcagttat atcggctgag gccagaatcc ttccatcgaa
      181 aaacgcaaaa gctccgaaaa gccatttcga atctttattc tgagaaagat gaggcatatt
      241 tgattatcga accttctgat attgccaatg acatcctttc ccagctcgag ccatttttcc
      301 aattggttcg acgtcaaaga ggggatttcg agtcgtattt caatgctgat ggtagcatta
      361 cttttacttt ttaaaccatt tatgataaat attggttagt tttggcaatg tcatagaaaa
      421 tttttgcctt aaagaagctg gtagaaattc ggctaaagac atcattcgat ataagatgaa
      481 accatataat tgccaaggca aagtctttag ccaggtaatc ggatgaaatt ttttatctac
      541 tctttgatat cccaatcttt cggcatatct tccaactact tgatcggcag ttttgatttc
      601 cttttgtttc atttctttca tccatttacc actcgaagag gtttgaatag gagattgaag
      661 tcctgaatga aattgattta ataactcagg gtcaacaaat tcggaatact tcgatgaata
      721 agtccaaaaa tcgagcatgg tttcatcata aggcagtcca agaaaagtac atgtatggct
      781 taagatttcc cgaggctttc tagttaaatc ttcatatctt atgatgagag tttgctctgg
      841 aaattttttt tcgaactgat aagccaaccg agccacgtat ttccatctat aagcctgcaa
      901 ggctataata ggagcctcaa atcggaattt tttaaccgaa agaaaattat cccgataatc
      961 cctaaaaata aaaataaatt tggcctttgg aaatatcttc ctcaaccgaa aagcatgggt
     1021 agcaaaaatg gggtttttat cacctatatg ggttatgatt tccttgccgg atactact
//
LOCUS       DAORPD010000748         1078 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_29455_length_1078_cov_1.991202, whole genome shotgun sequence.
ACCESSION   DAORPD010000748 DAORPD010000000
VERSION     DAORPD010000748.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29455_length_1078_cov_1.991202"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="PK976_08290"
     CDS             complement(<1..410)
                     /locus_tag="PK976_08290"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HRC67927.1"
                     /translation="MRKKVFSLVAALGFIMVIIPAFGQSNVISEIRQVGSFQTIQNNS
                     SVDILLLQGNSFEVKIEADSEIISYIDTEERNSTLYIDVKKRPGSRKIKVMRAIITVP
                     NLNVIRINGSGDVVSMNQLNFPRFSITLNGSGDA"
     gene            535..>1078
                     /locus_tag="PK976_08295"
     CDS             535..>1078
                     /locus_tag="PK976_08295"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HRC67928.1"
                     /translation="MALRHSFEKTGNFFFRYRGQIPVIIFISLIPAMFFTDYSNLDPS
                     IAKCLTAISIVLAATGFLVRIITIGTTPAGTSGRNTKSQVAEKLNTSGIYSIVRHPLY
                     LGNYLIWAGLTLFVFNFWYFLVISLVFWIYYERIMFAEEQFLRRKFGSAYTDWADKTP
                     AFIPNFGKYIPSNPHCRYYGV"
BASE COUNT          322 a          229 c          174 g          353 t
ORIGIN      
        1 tcggcatctc cacttccatt gagtgttata ctgaaacgag gaaaattcaa ttgattcatg
       61 ctcaccacat cacccgagcc attgattcta atcacattca aattaggcac agtaataatt
      121 gcacgcatca cctttatctt tctcgacccg ggcctttttt ttacatcaat gtaaagtgtg
      181 gaatttcttt cttcggtatc gatataagaa atgatttcgg agtcggcttc aatcttgact
      241 tcgaaactat tgccctgtaa aagaagaata tctaccgatg aattgttttg aatggtttga
      301 aatgaaccca cctggcgtat ttccgaaatt acattcgatt gcccaaaagc aggaattatt
      361 accataataa aacctaaggc cgccaccaac gaaaaaactt tttttctcat agcttttaat
      421 ttatccagtt taaacaaaat tacgactcca taaacctgca aaagttacac atgagacaaa
      481 atattttaca atttgcccag atcgaaatca ttacttttgt acaaaattag agttatggct
      541 ctgcgacatt ctttcgagaa aacaggtaat ttctttttcc gataccgtgg gcaaataccc
      601 gtaattattt ttatttcgtt aattccggcg atgttcttta ccgattatag taacctcgat
      661 ccttcaattg ccaaatgttt gaccgcaatt tcaattgttt tagctgcaac cgggttttta
      721 gtcagaataa taactattgg cacaactccg gcaggaactt cgggtagaaa tactaaatcg
      781 caagtagccg aaaaattgaa tacctcaggc atttattcca tcgtacgcca tcccttatat
      841 ctcggaaact atctcatatg ggccggactc accctgttcg ttttcaattt ctggtatttt
      901 ctggttattt cactcgtttt ctggatttat tacgaaagaa ttatgtttgc cgaagaacag
      961 tttcttcgcc gaaaatttgg ttctgcatat accgattggg cagacaaaac accggctttt
     1021 attccaaatt ttggaaaata cattccttct aacccacatt gcaggtatta tggagtta
//
LOCUS       DAORPD010000749         1077 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_29504_length_1077_cov_2.110568, whole genome shotgun sequence.
ACCESSION   DAORPD010000749 DAORPD010000000
VERSION     DAORPD010000749.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29504_length_1077_cov_2.110568"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..>1077)
                     /locus_tag="PK976_08300"
     CDS             complement(59..>1077)
                     /locus_tag="PK976_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxylase"
                     /protein_id="HRC67929.1"
                     /translation="LANIDILDTSILNFAGGPGAPAFEIIQIFCNKLNIETDLNLKVI
                     GNINEKLGEIRKSLSTYDTAPYLPRPFHIATDDIPLEIHNLFDKAIAAAQNKNQDELL
                     ESCHAIEDYFQLPPPDEKIREAEIPGGMYTNMLSQLKQVRLENLLPRTLELIPEVRLD
                     AGLPPLVTPTSQIIGSQAVNCALDETKGQPMYTNRSLQFVNLVKGIYGKTPYPIDPEF
                     RYKIAGVREETPYDFRFYKKQPNPVFEEYGGIKLANNEKEELLLELFPNVAADFLKNR
                     VEQIYMAKIHQIEEEKHRKLEEEKAAYEKLTPEEKRQRLLNGLYNYKWTSYQDDEEEA
                     LGHS"
BASE COUNT          281 a          192 c          229 g          375 t
ORIGIN      
        1 tggtactttt gtaaaatatt gatatgcaat tataggatca ggagttctga aatttcaatt
       61 atgagtggcc taaagcttct tcttcatcat cctgataaga agtccattta taattatata
      121 acccattaag caatctttgg cgcttttctt cgggagttaa tttttcgtaa gctgcttttt
      181 cttcttctaa tttcctatgt ttctcttctt caatctgatg aatttttgcc atataaattt
      241 gttcgacccg gtttttcagg aaatcggcag ctacgttggg aaaaagttct agtaataatt
      301 cttccttttc attatttgcc agttttattc cgccatattc ttcgaatacg ggattaggtt
      361 gttttttata aaatcgaaaa tcgtaaggtg tttcttctct aacgccggca attttatatc
      421 tgaattcagg atctattgga taaggcgttt tcccataaat gcctttcacc agattgacga
      481 attgcaatga gcggtttgtg tacataggtt ggcctttagt ttcgtcgagc gcgcaattta
      541 cggcttgcga accgataatt tggctggtag gcgtaaccag agggggcagc cctgcatcca
      601 acctaacttc gggaattaat tcgagtgtac gtggtaaaag attttcgagt cttacctgtt
      661 ttaactgcga taacatattg gtgtacattc cgccaggaat ttctgcttct ctaatttttt
      721 cgtcgggagg aggtagttga aaataatctt cgatggcatg gcaggattcg agaagctcat
      781 cctgattttt attctgggca gccgctatcg ctttatcgaa aaggttatga atttcgagag
      841 ggatatcatc ggtagcaata tggaaaggcc gagggagata aggagcagta tcgtaagtcg
      901 aaagcgattt tctgatttca ccgagtttct cgttgatatt acctataact ttcaaattca
      961 aatctgtttc tatgtttaat ttgttgcaga agatttggat gatttcgaag gctggggctc
     1021 ctggacctcc ggcaaaattc agaatcgagg tatcgagaat atcgatattg gccaaaa
//
LOCUS       DAORPD010000750         1075 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_29611_length_1075_cov_2.061765, whole genome shotgun sequence.
ACCESSION   DAORPD010000750 DAORPD010000000
VERSION     DAORPD010000750.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29611_length_1075_cov_2.061765"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1075)
                     /gene="dnaB"
                     /locus_tag="PK976_08305"
     CDS             complement(<1..>1075)
                     /gene="dnaB"
                     /locus_tag="PK976_08305"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HRC67930.1"
                     /translation="STEIIHDAYEDSMDALDLLDKAEMALFSISEENFRRDYEKMDSL
                     VRKAIDEIRKAYEQEDALTGVPSGFTDLDRITGGWQKSDLIVIASRPGMGKTAFILSM
                     ARNIALMKKRPVAIFSLEMSAIQLVTRLISAESGLSAEKLRSGQLKDFEWEQLNVKIT
                     PLLEAPIYIDDTPSLSIFELRAKCRRLKEQYDISIVMIDYIQLMSTGVENRGNREQEI
                     ALISRSLKALAKELDIPVIILSQLNRSVETRGGTKKPILSDLRESGAIEQDADLVLFI
                     YRPEYYKIETDEKGSTHNKADIIIAKHRNGKTDEVRLQFIDKFARFTDDVDITQITSN
                     LSMGVNYQGPTLTLPSKMNDDHP"
BASE COUNT          260 a          231 c          255 g          329 t
ORIGIN      
        1 tcgggatgat catcattcat tttagagggc agggtcaggg taggcccttg atagtttact
       61 cccatggaga ggttggaagt aatctgagtg atgtcgacat cgtcagtaaa tctggcaaat
      121 ttatcgataa attgcaatcg aacttcgtcg gttttcccgt ttcgatgctt ggcaatgatg
      181 atatcggcct tgttgtgtgt agagcctttt tcgtcggttt cgattttata atattcagga
      241 cgatagataa acagtacgag atcggcgtct tgttcgatag ctcctgattc acgcaagtcg
      301 ctcaagatgg gctttttggt tccgccacgg gtttcgacgc tgcgattcaa ttgtgaaaga
      361 atgatgacag gaatgtccaa ttctttagct aaggctttta gagaacggct tatcaatgca
      421 atttcctgtt cgcggtttcc gcgattttcc actcctgtgc tcatcaactg gatgtaatct
      481 atcattacta tactaatgtc gtattgttct ttcagccggc ggcattttgc ccttagttcg
      541 aatatggaga gggagggggt gtcgtcgata taaatgggag cttcgagcag aggagtaatt
      601 tttacgttga gttgttccca ttcgaaatcc ttaagctgtc ccgatcggag tttttcggct
      661 gaaagacccg attcggccga gataagacgt gtaaccagtt gtatggccga catttccaaa
      721 gagaatatgg ccaccggacg ttttttcatc aaagctatgt tgcgagccat gcttaggata
      781 aatgccgttt ttcccatgcc aggccttgag gcgattacaa tgaggtcaga tttttgccaa
      841 cctccggtga ttctatccag atcggtaaat cccgaaggaa ctcccgttag ggcatcctcc
      901 tgttcatagg cttttctaat ttcatcgatg gccttgcgca ccaaagaatc cattttttcg
      961 taatcgcgcc ggaaattttc ctcgctgatt gaaaacaagg ccatttcggc cttatcaagc
     1021 aaatcgagcg catccatcga atcttcataa gcatcgtgaa taatctcggt cgaaa
//
LOCUS       DAORPD010000751         1074 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_29651_length_1074_cov_2.888126, whole genome shotgun sequence.
ACCESSION   DAORPD010000751 DAORPD010000000
VERSION     DAORPD010000751.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29651_length_1074_cov_2.888126"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="PK976_08310"
     CDS             <1..488
                     /locus_tag="PK976_08310"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HRC67931.1"
                     /translation="GVSAQKFAYVDSEYILQNIPEYNDALAQIDEFAAQWQQELEAKF
                     AAIDKMYKDYQAEAVLLPEEMKRKREDEIMKAEKAAKDLQKQRFGKDGDLFKKRQELV
                     KPIQDKIYSAIERIAQAKNYAMIFDKAGNVTVMYADAKYDISDEVLEEMGYGFNTRKN
                     K"
     gene            640..>1074
                     /locus_tag="PK976_08315"
     CDS             640..>1074
                     /locus_tag="PK976_08315"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HRC67932.1"
                     /translation="MKKTFVKFFVLATCVFAFGGVSAQKFGHIDFAKLFVVMPGQDTI
                     QEKYRQYADGLRVQMEAMQTEWETKITDYQAKSSSMTDLIRRTKEKELQDLQSRIEAF
                     QQQASIDLQNKETELTSPLIERAKKAIQDVAKENGYTYVFNSA"
BASE COUNT          382 a          200 c          225 g          267 t
ORIGIN      
        1 ttggagtcag tgctcaaaaa tttgcctatg tagattcgga atatattctt caaaatatcc
       61 ccgaatataa tgacgccctt gctcaaatcg atgaatttgc cgcccaatgg cagcaggaac
      121 tcgaagctaa atttgcagcc atcgataaga tgtataaaga ttaccaggcc gaggctgttt
      181 tattgcccga agagatgaaa cgcaaacgtg aagacgaaat tatgaaggcc gaaaaagccg
      241 caaaagatct gcaaaaacaa cgcttcggta aagacggcga cttattcaag aaacgtcaag
      301 agctggttaa acccattcaa gataaaatct attcggctat cgaaagaatc gctcaagcga
      361 aaaattatgc catgatattc gataaggctg gaaatgttac agtgatgtat gccgatgcca
      421 aatatgatat cagcgacgaa gtactcgaag aaatgggtta tggcttcaat acacgaaaga
      481 ataagtaaaa accactttgt aacacaataa atattacatg ctatctgtcc tgaacttttt
      541 tgcgaaaaga agaatgtttt tattaaaata ggaactcgag gaaatagtct gctcgaaatt
      601 atttaatttt ggcgcataat cttaaaaaga acattaagaa tgaagaagac ttttgttaaa
      661 tttttcgtac ttgctacatg tgtatttgct tttggaggag tttcggctca aaaattcggc
      721 cacattgatt ttgccaaact tttcgtggtg atgccaggac aggacaccat acaggaaaag
      781 tatcgccaat atgccgatgg gttgagggtg cagatggaag ccatgcaaac ggaatgggaa
      841 accaaaataa cagattatca ggcaaaatct tccagcatga ctgatttgat ccgcaggaca
      901 aaggaaaagg aacttcaaga tctgcaaagc cgtatcgaag cttttcagca gcaagccagc
      961 atcgatttac agaataaaga aaccgaactt acttctcctc tgatcgagag agcaaaaaaa
     1021 gccattcagg acgtggctaa agaaaacgga tacacttacg tattcaatag tgcc
//
LOCUS       DAORPD010000752         1074 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_29655_length_1074_cov_2.450442, whole genome shotgun sequence.
ACCESSION   DAORPD010000752 DAORPD010000000
VERSION     DAORPD010000752.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29655_length_1074_cov_2.450442"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /locus_tag="PK976_08320"
     CDS             <1..847
                     /locus_tag="PK976_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRC67933.1"
                     /translation="PQGDSLDGLLYKPENFDSTRQYPMIVYFYEKSSQDLYSYFSPLP
                     SRSIINPTYLVSNGYLVFVPDIVYKSDNPGNDAYDCVMSGVLSLSSSGFVDKDRIGLQ
                     GHSWGGYQVAYIITRTDLFACAMAGAPVANMISAYGGIRGESGKSRMIQYERSQSRIG
                     GSLWEKPLKFINNSPLFFADNIHTPLLILHNDNDGAVPWSQGLELFSAMRRLQKPCWM
                     LVYNKEDHNLKEKSWGNRMDLTQRMYQFFDHYLKGSPAPQWMKKGIPAIRKSIDNGLK
                     FDEND"
     gene            859..>1074
                     /locus_tag="PK976_08325"
     CDS             859..>1074
                     /locus_tag="PK976_08325"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRC67934.1"
                     /translation="MYEHVTNLRVRYVEVDSMGYVYHGNYASYYEVGRTELMRNLGSS
                     YKKLEDEGVLMPVIHLECHYIRPARYDD"
BASE COUNT          319 a          198 c          239 g          318 t
ORIGIN      
        1 acctcaaggt gattctttag atggtttact ctataaacct gaaaatttcg attcgacaag
       61 gcaatatcca atgattgtct atttctatga gaagagttcg caagatctat acagctattt
      121 ttcaccttta ccatcgcggt ctattattaa tcctacttat ctcgttagca atggatatct
      181 ggtatttgtg cccgatatag tatataagag cgacaatccg ggaaatgatg cttacgactg
      241 tgtaatgagc ggagttctat ccttatcgtc ctcaggcttt gtggataagg atcgtattgg
      301 attacagggt catagctggg gtggatatca agtggcttat attattacac gcaccgacct
      361 attcgcctgt gccatggctg gtgcaccagt tgccaatatg attagtgctt acggtggaat
      421 acgaggggaa tcgggaaaaa gtcggatgat acaatacgaa agatcacaaa gcaggattgg
      481 aggtagcctt tgggagaaac ctttgaaatt tatcaataat tctcctttat tttttgccga
      541 taacattcat actccccttc tcatcttgca taacgataat gatggagctg taccctggtc
      601 gcaggggctg gagttattta gcgctatgag gcgtttacag aaaccatgct ggatgcttgt
      661 atacaataaa gaagaccaca acctgaagga aaaatcatgg ggaaaccgca tggatttgac
      721 tcaacgaatg taccagtttt tcgatcatta tttaaaggga agtcctgctc ctcagtggat
      781 gaagaaaggc attccagcta ttcgaaaaag tatcgataat ggattgaaat tcgatgaaaa
      841 tgattaatac tcgctattat gtatgaacac gtcaccaacc ttagagttag atatgtcgaa
      901 gtagattcga tgggttatgt ttatcatggg aattatgcct cctattatga agtcggacgc
      961 accgaactta tgcgcaacct tggaagttcc tataaaaaat tggaagatga aggagtattg
     1021 atgcctgtta tccatctcga atgccattac ataaggccag ctaggtatga cgat
//
LOCUS       DAORPD010000753         1074 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_29668_length_1074_cov_1.870461, whole genome shotgun sequence.
ACCESSION   DAORPD010000753 DAORPD010000000
VERSION     DAORPD010000753.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29668_length_1074_cov_1.870461"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="PK976_08330"
     CDS             complement(<1..752)
                     /locus_tag="PK976_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008147311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRC67935.1"
                     /translation="MTLSQKIADLKQRIRVASEGGGPKAIESQKAKGKLTARERILAL
                     IDPKSFHEYDLFVANAGKDFDMDKKFLPGDGVVTGTGTINGFPICIFAQDFTVAGGSL
                     GYAHAKKITKIMDHAMKLKVPLIGINDSGGARIQEGVNALAGYGDIFYRNTMASGVIP
                     QISVILGPCAGGAVYSPALTDFVFVVDKISQMFITGPEVIKTVLGEEISKEDLGGAKV
                     QAQITGNAHFYAKSEEECFAQIKELMSYIPWN"
     gene            940..>1074
                     /locus_tag="PK976_08335"
     CDS             940..>1074
                     /locus_tag="PK976_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HRC67936.1"
                     /translation="MNIEELREYCLSKNQAEECLPFDDNSLVFKVKDKIFAILSLTQP
                     F"
BASE COUNT          278 a          242 c          201 g          353 t
ORIGIN      
        1 ttgttccatg ggatatagct catcagttcc ttgatttggg caaagcattc ttcttcgctt
       61 ttagcataga aatgagcatt gccagtgatt tgagcctgca ctttagcccc tccaagatct
      121 tcctttgaga tttcttctcc taaaacagtt ttaattacct cgggcccagt gatgaacatt
      181 tgagagattt tgtctacaac aaatacaaaa tcggtgaggg caggcgaata tactgcgcct
      241 ccagcgcaag gacccaatat aaccgagatt tgtggaataa cccccgatgc catggtattg
      301 cgatagaaaa tatcgccata gccggctagt gcattcacac cttcctgaat gcgcgctcct
      361 cccgaatcgt tgatgccaat caaggggact tttaacttca tggcgtgatc catgatttta
      421 gttattttct tcgcatgggc ataccctaac gaccccccag ctaccgtaaa gtcctgagca
      481 aaaatacaaa tagggaaacc attaatggta cccgtccctg ttactactcc atcgccggga
      541 agaaatttct tgtccatgtc aaaatctttc ccggcattgg ctacaaaaag atcatattcg
      601 tgaaatgact ttggatctat tagagctaat attcgctccc gtgcagtcaa tttacccttc
      661 gctttctgac tttctatagc cttggggccg cctccctccg atgctaccct tatcctttgc
      721 ttgaggtcgg caatcttttg gcttaatgtc attccttcgt tgttcaattg tgtgaggttt
      781 tagtaagatt tttccaaagg gaagcaaatt tataggaaaa ccacctttct tcaaacgcta
      841 tttttattta ctctcttttg gtcgaaaata atttttactg tttcgactgg ttatgtgtat
      901 gttatatgat tttcttactt ttgttaataa atgttaatca tgaatatcga agaattgcgt
      961 gagtattgcc tttcgaagaa tcaagccgaa gaatgcctgc ctttcgatga taactctttg
     1021 gtttttaaag ttaaagataa gatttttgct atcttgagcc tcacccaacc tttc
//
LOCUS       DAORPD010000754         1073 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_29708_length_1073_cov_2.919450, whole genome shotgun sequence.
ACCESSION   DAORPD010000754 DAORPD010000000
VERSION     DAORPD010000754.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29708_length_1073_cov_2.919450"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="PK976_08340"
     CDS             <1..634
                     /locus_tag="PK976_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67937.1"
                     /translation="NLELVDSSHYIDKKWPTEHYDEYGRKMVARQIALAMRNIYPAHF
                     SDVFMKSDFTCDFEAPSPFFVTLGRDSTCSHSGKFAEKLDTNNAFGARFVRHYFEVKT
                     LNPRKITASGWFTGIYRPREVKLIISIENDKKQPKLWTATVSKDIIEYGKWDSLQVNA
                     NIPENFGDNDVIRIYFWYTGKNKVWIDDVNILLQTENLNAASDTILLIKN"
     gene            704..>1073
                     /locus_tag="PK976_08345"
     CDS             704..>1073
                     /locus_tag="PK976_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67938.1"
                     /translation="MKKIKHPISAASLLLCVIVLISVRLSHPPKNILSYDTFGYYIYL
                     PARHIYHDPGIHNFEWVKEINQKYNNTPTFYQFSEGINGQKVIRFNRGISYLLAPGFY
                     VGHAWAKLSGAPQDGFSKPYQ"
BASE COUNT          347 a          229 c          192 g          305 t
ORIGIN      
        1 taatctcgag ttggtagatt cttcacacta catcgacaaa aaatggccta ccgagcatta
       61 cgatgaatat ggtcgaaaaa tggtagcccg tcagatagct ctggccatga gaaatattta
      121 tcctgcccat ttttccgatg tattcatgaa atcggatttc acttgcgatt tcgaagcccc
      181 atcacctttt tttgttacac ttggaagaga ttcgacttgc tctcactcag gaaaatttgc
      241 cgaaaaactc gataccaata acgcatttgg agccagattc gttagacatt atttcgaagt
      301 gaagactctt aaccctcgaa aaattaccgc cagcggatgg tttacgggca tctatcggcc
      361 tcgtgaagta aaactcatca tcagcatcga aaatgataaa aaacagccca aactctggac
      421 tgcaaccgtt tcgaaagata tcatcgaata tgggaaatgg gattcgctgc aagtgaatgc
      481 taatatcccc gaaaactttg gcgataatga cgtaataaga atatatttct ggtacacggg
      541 aaaaaataaa gtctggatag atgacgtgaa cattctgttg caaactgaga atctgaatgc
      601 tgcatccgat acaattttat tgattaaaaa ttaactactc taaatatatt tcctatcttt
      661 cgaaaataaa tccttatcat tatttgagtt aaaacttcat cctatgaaaa agataaaaca
      721 tcccatatcg gcagcaagtc tgcttttgtg cgtgatcgta ttaattagcg ttcgattgag
      781 ccatcctcca aagaatatat tatcgtacga cactttcggc tattatattt atttacccgc
      841 ccgccatata taccacgatc cggggataca caacttcgaa tgggtaaaag aaataaacca
      901 aaaatataat aatactccaa cattctatca gttttcagaa ggaataaatg ggcagaaagt
      961 catacgtttt aaccgtggta tttcctacct tctggctccc ggattctatg tcggtcatgc
     1021 ctgggcaaaa ttaagcggag cccctcagga tggcttctcc aaaccctatc aac
//
LOCUS       DAORPD010000755         1071 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_29798_length_1071_cov_2.390748, whole genome shotgun sequence.
ACCESSION   DAORPD010000755 DAORPD010000000
VERSION     DAORPD010000755.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29798_length_1071_cov_2.390748"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="PK976_08350"
     CDS             <1..718
                     /locus_tag="PK976_08350"
                     /inference="COORDINATES: protein motif:HMM:NF014307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HRC67939.1"
                     /translation="VAAAMGGFLYGNRAMLTGGILVGSAGTILTILMCKAMNRSIINV
                     IAGAFGGKSKEGAADIDRTVREINLSDAAILLCYSQKVYIVPGYGLAVAQAQHLCHEL
                     DLLLSSKGVEVKYGIHPVAGRMPGHMNVLLAEADVPYEKLVEAEDANNEMPNTDVVIV
                     IGANDVVNPAAEEDPSSPIYGMPVIRVWEAKNVIVMKRSMAKGYAAIENNLFYHPRTR
                     MLFGDAKVSLQKLIAEIKSL"
BASE COUNT          315 a          195 c          241 g          320 t
ORIGIN      
        1 ggttgctgcg gccatgggag gatttcttta cggcaaccga gccatgctca ccggtggtat
       61 actcgttgga tcggctggta ccattctcac aattttaatg tgcaaggcta tgaatcgttc
      121 tataataaat gtcattgcgg gggctttcgg aggcaaaagc aaagaaggcg ctgcagacat
      181 cgatagaacc gttagagaaa tcaacttaag tgatgcggct attcttcttt gctattccca
      241 aaaggtatat atcgttccgg gttatggact ggccgttgct caggctcagc atctctgtca
      301 tgaactggat cttttgttat ctagtaaggg ggtggaagtg aaatatggaa ttcatcccgt
      361 tgctggacgt atgcccgggc acatgaacgt tttactggcc gaggcagatg ttccttacga
      421 aaaattagtc gaggccgaag atgccaacaa cgaaatgcct aataccgatg tggtaattgt
      481 aataggagct aatgatgttg taaatcccgc agccgaagaa gatccttcga gccctattta
      541 tggtatgcct gtaatcaggg tttgggaagc aaaaaatgtc attgttatga aacgttcgat
      601 ggcaaaagga tatgccgcta ttgaaaataa tcttttttat catcctcgca ctcgaatgct
      661 ttttggtgat gccaaagtaa gcttgcaaaa actcatcgct gaaattaaaa gtctgtaatc
      721 tttacgcttt ggattgaaaa gtcggccatg tgggccgact tttcgcctct cgatatactc
      781 cttccataac aagtataatg gctccgattt gcgtaaattc agtttcaatg attatttatt
      841 atatccattc gtcgaaattc gatgctactt ttgtcccgtc ataactaacg ggattgatat
      901 gcaattatag agtcaggagt tctaaggata caaagcgaaa taaatatggc aaaatgaaga
      961 agataaaata agacactggc tcgaaatgcg tttaaaattt ttaatctgaa agtatttttc
     1021 gatgtggttc cttaaatgta aaatattaaa ggattgcaaa atataagttt t
//
LOCUS       DAORPD010000756         1069 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_29903_length_1069_cov_2.453649, whole genome shotgun sequence.
ACCESSION   DAORPD010000756 DAORPD010000000
VERSION     DAORPD010000756.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29903_length_1069_cov_2.453649"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /locus_tag="PK976_08355"
     CDS             <1..978
                     /locus_tag="PK976_08355"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HRC67940.1"
                     /translation="FVPPSFYPIFLSVSSVKQLYLLSTLITYRTIMALIFIVRRIKII
                     FLCKFAPRYLTYKPLKEISMMNIAVMQLDLKWEEPLMNRHKVENLLTSLPNETDLILL
                     PEFFTTGFSVKNIGLAEPMNGPTVSWMQKIAKEMNCILAGTIPIIDHGKYYNRALYVS
                     REGVMASYNKRHLFSLGSEHQLYTPGNKNVIVEYKNAKIQPQICYDLRFPVWSRNRYS
                     NFQFAYDILIYHANWPDPRGCVWEQLLIARAIENQAYLVGINRCGQDGEGLSYSAPSM
                     IINPKGNIIAQMEPAKEGVLSANIDLGELKRFREKFFIAPDWDNFDVSP"
BASE COUNT          325 a          172 c          207 g          365 t
ORIGIN      
        1 tttgtccctc cttcttttta tccaattttt ttgtcggtca gtagtgttaa acaattgtac
       61 cttttgagta cgttgataac ttatagaaca attatggcct tgatttttat tgtgcggcgt
      121 ataaaaatta tttttttatg taagtttgcc ccaagatatc tcacatacaa acctctaaag
      181 gaaatatcca tgatgaatat tgccgtaatg caattagatt tgaaatggga ggagccttta
      241 atgaataggc ataaagtaga aaatttactt acaagtctcc ctaatgaaac tgatttgatt
      301 ttattaccag aattttttac tacgggattt tcagttaaaa atatcggttt ggctgaaccc
      361 atgaatggac ctactgtttc atggatgcag aaaatagcaa aagaaatgaa ttgtatactg
      421 gcaggcacta ttcccattat agatcatgga aaatactaca atagggctct ttatgtatct
      481 agagagggtg ttatggccag ttataacaag cgccatcttt tcagtttagg gagtgaacac
      541 caattatata cccctggcaa taaaaatgtg attgttgaat ataaaaatgc gaagattcag
      601 ccgcagatat gttatgacct tcgatttcct gtttggagtc gcaaccgata ttctaatttc
      661 cagtttgctt atgatatttt gatttatcat gccaattggc cagatccacg aggatgtgtt
      721 tgggaacaat tattaattgc gcgtgctata gaaaatcagg cttatttggt gggcataaat
      781 cgttgtggac aagatggtga gggcctttcg tattcagctc cttctatgat tattaacccc
      841 aaggggaata ttattgctca aatggaacct gccaaggaag gtgttttgtc ggctaatatc
      901 gatttgggtg agctgaaacg ttttcgcgaa aaattcttca tcgcacccga ttgggataat
      961 tttgatgttt ccccttgaag attttttagt taaagacatt ttttagaaca acggaaaaga
     1021 atctaatctt ctaaaatttg gttcgttaac aaaatgactt taaatctta
//
LOCUS       DAORPD010000757         1069 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_29905_length_1069_cov_2.364892, whole genome shotgun sequence.
ACCESSION   DAORPD010000757 DAORPD010000000
VERSION     DAORPD010000757.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29905_length_1069_cov_2.364892"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1069)
                     /locus_tag="PK976_08360"
     CDS             complement(<1..>1069)
                     /locus_tag="PK976_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family peptidase"
                     /protein_id="HRC67941.1"
                     /translation="PLDPLLFIGKILQDTRKPEIRGIAFYPVKGKGVVNNTSDPSHIY
                     ISHKKTGEALPLSKQIEAWGLIGIGVKAYDRMNGQNNIYGVKQIRLFVDSMLIYSSKI
                     DRFSFEKTRMINSFVDFEEWRLRNSFFMKSYIEPGNNLPVYNKVINHGYIDIDQERNY
                     MMRYELEDYYGNVLTYPFVVKGKNQNIKPILPCKNYMAWNLDNSFVDYDFTLRIPARN
                     LYSDFCFVHTSTPSTTYYSAIHHVHPSPVPLDDQAYLWIKLNADTLDRRENLGIVEIK
                     KTGKSSWIGGDYKNGGVEVSVKELGGKFAIDTDTVAPKITPLQPEKWVSSGKIRLQLS
                     DDKSGITSFRGEIDGKYALFTH"
BASE COUNT          290 a          211 c          221 g          347 t
ORIGIN      
        1 gtgggtaaat aaagcgtact ttccatcaat ttctcctcga aaagaggtta ttccgctctt
       61 atcatcagat agctgaaggc gtatttttcc ggaagaaacc catttttcgg gttggagtgg
      121 ggtaattttg ggtgcaacgg tgtctgtatc gatggcgaat tttcccccta attccttgac
      181 agatacttcc acgcctccat ttttataatc acctccaatc catgaacttt ttcccgtttt
      241 ttttatttcc actatgccta aattttctct gcgatcgagt gtgtcagcat tgagtttgat
      301 ccacaagtat gcctgatcat cgagtggaac gggcgatgga tgaacgtgat gaatggccga
      361 ataataagtt gtggaaggtg tcgaagtgtg gacaaaacaa aaatcggaat agagatttct
      421 tgcaggaatt cgaagagtga agtcataatc gacgaacgaa ttgtcgagat tccaagccat
      481 atagttttta cagggaagta tgggctttat attttggttt ttccctttca ccacaaatgg
      541 gtaagtgagc acattaccat aataatcttc cagttcgtaa cgcatcatgt agttgcgttc
      601 ctgatcgatg tcgatgtatc cgtggttaat aaccttgttg tacacaggca ggttgttgcc
      661 aggttcgata tatgatttca tgaaaaacga atttctcaat cgccattctt caaaatcgac
      721 aaaagaattg atcattcgtg ttttttcgaa cgagaaccga tctatcttac tactataaat
      781 caacatgctg tccacaaaaa gtctgatctg cttcactccg taaatattgt tttgtccatt
      841 catcctgtcg taagctttca ctccaatgcc tataagtccc cacgcttcaa tttgtttgga
      901 tagtggaagc gcttctcctg tttttttgtg gctaatgtag atatgagaag gatcagaagt
      961 gttattcacg acgccttttc cctttacggg gtaaaaggct ataccacgaa tttcaggttt
     1021 tcgagtatct tgtaatattt ttccgataaa aagaagagga tccaacggg
//
LOCUS       DAORPD010000758         1069 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_29931_length_1069_cov_1.696252, whole genome shotgun sequence.
ACCESSION   DAORPD010000758 DAORPD010000000
VERSION     DAORPD010000758.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29931_length_1069_cov_1.696252"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="PK976_08365"
     CDS             complement(<1..448)
                     /locus_tag="PK976_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HRC67942.1"
                     /translation="MDLEYLIQKKVFDADSIELQRLLAYWRFKNFKIVFTNGCFDLVH
                     LGHIDYLIRAADLGDILVIGLNSNESARRIKGEGRPIMDQKSRSSLLASFIFVDAVII
                     FEEDTPIELIRTIQPDILVKGADYKPEKIIGSDIVTENGGEVVTLPL"
     gene            complement(411..>1069)
                     /locus_tag="PK976_08370"
     CDS             complement(411..>1069)
                     /locus_tag="PK976_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67943.1"
                     /translation="TLDLIVFILLFILNLVLQIDLIRQYVNEKIYTPLMHSLGSVENV
                     GLLKWAILGAFVLLIVLLIVFRKKFYRFRFVRKLKDMGKGVWDGIWSILKLNRPIEFI
                     FHTLFIWVAYFFMSWIVFLSLPETSGLGLGAGLAVLVFGSIGIILVQGGIGVYPAIVA
                     ETLVIYGISSIIGYAMGWLLWATQTIMLIIAGLSSLILLPVKNKVSYGFRIFNTEESI
                     "
BASE COUNT          367 a          232 c          193 g          277 t
ORIGIN      
        1 caagaggaag cgtaaccact tcaccgccat tttcggtaac tatgtcggaa ccaataattt
       61 tttcgggttt atagtcggct ccttttacca gaatatcggg ttgaatcgtt cgtataagtt
      121 cgataggagt atcttcctcg aaaataatta cagcatcgac gaatataaaa ctcgccaaga
      181 gcgatgacct gcttttctgg tccataatgg gtcggccttc gcctttaata cgccgggcac
      241 tttcattgga gttgaggcca ataacgagaa tatctcccag atcggcggcc cggataagat
      301 aatcgatgtg gcccagatga accagatcga aacagccatt agtaaaaacg atcttaaagt
      361 tcttgaatcg ccagtaagct aatagacgct gcagctcgat cgagtcagca tcaaatactt
      421 tcttctgtat taaatattct aaatccatac gatactttat ttttaacggg taatagaatc
      481 aatgatgata aaccagctat tatgagcata atagtttgcg ttgcccataa taaccagccc
      541 atggcataac caataatgga actgatgccg taaattacca gagtttcggc tacgatagct
      601 ggatatacac ctatgccgcc ctgtaccaga ataataccta tcgaaccaaa taccagcact
      661 gccaaaccag cacctaagcc taaaccggaa gtttcgggta agcttagaaa aactatccac
      721 gacataaaaa aatatgctac ccagataaac agagtatgaa aaataaattc gataggccgg
      781 tttaatttta agatagacca aatgccatcc catacccctt tacccatatc cttgagtttt
      841 cttacaaatc taaaacgata gaattttttt cgaaaaacaa tcagcaaaac gattaaaagc
      901 acaaaagcgc ctaaaatagc ccatttcaac aaaccaacgt tttcgaccga tcctaatgaa
      961 tgcatcaatg gagtataaat tttttcgttg acatactgac gaatgaggtc gatttgcaga
     1021 acaaggttta gaatgaacaa aagaataaaa acgatcagat cgagggttc
//
LOCUS       DAORPD010000759         1068 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_29970_length_1068_cov_3.084896, whole genome shotgun sequence.
ACCESSION   DAORPD010000759 DAORPD010000000
VERSION     DAORPD010000759.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29970_length_1068_cov_3.084896"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            165..>1068
                     /locus_tag="PK976_08375"
     CDS             165..>1068
                     /locus_tag="PK976_08375"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HRC67944.1"
                     /translation="MLHVHKILVLRFSSIGDIVLTTPVIRCMHRQLENAEIHYLTRTV
                     YMPLLEHNPYIHRLMGFDHSPQELLPRLKAEHYDFIVDLHRNFRTVALRLRLLRPSAS
                     FPKLNIRKWLMVNFHINTLPKIHIVDRYFMAIKRLGVLNDGKGLELFISQKDEVALSE
                     LPANFWNGYGVIAIGAKHYTKQIPVELATEIVSLAPLPFLLVGGIEDYDKGEQINHKI
                     PLKSYNICGKYSLPQTASIVKNAKIVITSDTSIMHIAAAFHKPIISIWGNTLPEFGMY
                     PYMPGNEEKSILIENKELKCRPCSK"
BASE COUNT          328 a          226 c          198 g          316 t
ORIGIN      
        1 gtcgatggct gggagtaaaa atttgcttac tcccactcga atggcctcct cgacaacttt
       61 cgacaaatcc tcttcaaatt cggacagata aagatgtgta tgggtatcta taattttcat
      121 ggcacaaaat taccagaaaa tcaaattttc taaatttgcg tcaaatgcta catgtgcata
      181 aaattcttgt gcttcgcttc agttcaattg gcgatatcgt tctcaccacg ccagttatcc
      241 gttgcatgca tcgtcaactc gaaaatgcag aaatacacta tttgacccga acagtataca
      301 tgcctttgct tgaacacaat ccttacattc atcggttaat gggcttcgat catagtcctc
      361 aggaattatt gcctcggctt aaagccgaac attacgattt catcgtcgat cttcaccgca
      421 attttcgcac tgtcgctctt aggcttcgtc ttctgaggcc atcggcaagc tttcctaaat
      481 taaacattcg aaaatggctg atggtgaatt ttcatatcaa tacactgcca aagattcata
      541 ttgtggatag atacttcatg gcaattaaac gtttgggagt tttgaatgat gggaaaggac
      601 tcgaattatt catttcacag aaagatgaag tagctctttc ggaattaccc gctaattttt
      661 ggaatggcta tggagtgatt gctatcggag caaagcatta taccaaacaa attcctgtcg
      721 aactggccac agaaatcgtt agcctggcgc ctttaccgtt tttgcttgtc ggtggaatcg
      781 aagattatga caaaggtgag caaatcaacc acaaaattcc attgaaatca tacaatatct
      841 gtggaaaata ttctctgccg caaactgcat ctattgttaa aaatgccaaa atcgtcatta
      901 cctctgatac aagcattatg catattgcag cagcttttca caaacccatc attagcatct
      961 ggggaaatac actgcctgaa tttggaatgt atccctacat gcctggaaac gaggaaaaat
     1021 ctattttgat agaaaataag gagttaaaat gccgaccatg cagtaaat
//
LOCUS       DAORPD010000760         1066 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_30084_length_1066_cov_2.893175, whole genome shotgun sequence.
ACCESSION   DAORPD010000760 DAORPD010000000
VERSION     DAORPD010000760.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30084_length_1066_cov_2.893175"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..868)
                     /locus_tag="PK976_08380"
     CDS             complement(77..868)
                     /locus_tag="PK976_08380"
                     /inference="COORDINATES: protein motif:HMM:NF033580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HRC67945.1"
                     /translation="MGNLGANFKKADPYTTGRPRKVDLREVINAIFYLNKTGCQWRYL
                     PKDFPHYTLISYYYHKWVDNNTIEQINTAIHQQLRKEMGRNEHPSAGIIDSQTVKGTP
                     ESTRESGFDGGKLIQGRKRHIVVDTTGYLIVALVHAANIYDGHAARQVLTTLFSIVDS
                     VKKIWADGGYRGEEFIQWVKEQFNCIFEVVEKKKTDKGFQVLPRRWVVERTFAWLGRS
                     RRLSKDYERKPTSSTGHVYMASIRLMLRRICKERVLLQGTSLEVA"
BASE COUNT          296 a          246 c          200 g          324 t
ORIGIN      
        1 agaagtaccg gattaaattt ttaaagatca taagctggtg ggttgaaact aagaagctgt
       61 acaaaaactc aattacctaa gctacctcta aagaagtccc ttgtaataat actcgctctt
      121 tacaaattct tcgcagcatg agtcgaattg aggccatata aacatgaccc gtactcgatg
      181 tgggtttccg ttcgtaatct ttgcttaagc ggcgagatcg cccgagccaa gcaaaggttc
      241 gctctacaac ccatcgccga ggtaaaacct gaaatccctt atccgttttc tttttttcaa
      301 caacttcaaa aatgcagtta aactgttcct tgacccattg aataaactct tcacctctat
      361 aaccaccatc cgcccagatt tttttgacgc tatccacgat tgaaaacaag gtggtcaaga
      421 cttggcgagc cgcatgacca tcgtagatat ttgccgcatg aaccagagca acaatgagat
      481 aacccgttgt atctacgaca atatgccgct tgcgaccctg aatcaactta ccaccgtcga
      541 acccggactc ccgggttgat tccggtgtcc ccttaacggt ttggctatcg ataatccctg
      601 cactcggatg ctcatttcga cccatttctt ttcggagttg ctgatgaatc gctgtgttga
      661 tttgctcgat agtattattg tcaacccatt tgtgataata gtaactaatt aaggtatagt
      721 ggggaaaatc tttaggcaaa taccgccatt gacaacccgt cttgttcagg taaaaaatgg
      781 catttattac ttcacggaga tcgactttac gtggccgacc tgtcgtataa ggatcggctt
      841 ttttaaaatt ggctccaaga tttcccattc ttcatctgtc aaatcactgg gatacctctt
      901 accaggaatt tcttttggcc aagccatgca gcacctcgaa tagaatctct gaaattaaaa
      961 taatcgccgg attttggctt attttatcaa aaatactata ataatatcaa tgtgttagtt
     1021 tttgtacggc ttcttattgg tcgctgttat ttctaattga tcgcgg
//
LOCUS       DAORPD010000761         1061 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_30368_length_1061_cov_2.390656, whole genome shotgun sequence.
ACCESSION   DAORPD010000761 DAORPD010000000
VERSION     DAORPD010000761.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30368_length_1061_cov_2.390656"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1061
                     /locus_tag="PK976_08385"
     CDS             <1..>1061
                     /locus_tag="PK976_08385"
                     /inference="COORDINATES: protein motif:HMM:NF013781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA mutase large subunit family protein"
                     /protein_id="HRC67946.1"
                     /translation="IIETTKDFPKVKSLTISGYLFGNSGSSIVQELAFSLSAAVEYFH
                     RLTDLGLSVSDISNKMRFIFSIGSNYFFEIAKLRAARMLWARVVNEFRPSDMDSCKMN
                     IHCVTSAYNKTLYDPYVNMLRTTTEAMAATLGGTDSLEVLPFDFPSGKTTEFSERIAR
                     NQQLILKEEAGFDKIADPSAGAYYIENLTDNIAREAWKLFLEIENQGGYYQALRVGKI
                     QEMISGNALKREAAIASRKETLVGTNQYPDLNEYIDKELDPSIYTKPQIPTAAYTIQP
                     LIPYRGSMPMEELRYKTDFYSKNHKRPAVFLLLIGNVAMRQARAQFSSNFFGCAGFQI
                     ITGNPVASVDEGIRAAHES"
BASE COUNT          317 a          239 c          218 g          287 t
ORIGIN      
        1 aattatcgaa accactaaag attttccaaa agtaaaatct cttactatat ctggatactt
       61 attcggaaat tcaggaagca gtattgtaca ggaactcgcc tttagtttgt cggcagcagt
      121 agaatatttc catcgtttaa cagatttggg attgagtgtt tctgatattt cgaacaaaat
      181 gcgctttatt ttctccattg gttcgaatta tttctttgag atagccaagt tgcgagctgc
      241 acgcatgtta tgggcaagag tcgtaaatga atttcgaccc agcgacatgg attcctgcaa
      301 gatgaatata cactgtgtta catcagccta taacaaaacc ctgtatgatc cttatgtaaa
      361 catgttgcgt acaacaaccg aagccatggc agcaacgctg ggcggaaccg attcgctcga
      421 ggttttgcct ttcgattttc cttcgggtaa aaccactgaa tttagcgaac gtattgcccg
      481 caatcaacaa ttgattctga aagaagaagc cggcttcgac aaaattgccg acccctccgc
      541 aggcgcttat tatatcgaaa accttaccga taacatagct cgtgaggctt ggaaactttt
      601 cctcgaaatc gaaaatcaag gaggttatta tcaagctctt agggtaggaa agattcagga
      661 aatgatttcg ggaaatgccc tcaaacgcga agccgctatt gcctcaagaa aagaaaccct
      721 ggttggcacc aatcagtatc ccgatctgaa cgaatatatc gacaaggagc ttgatccatc
      781 catctataca aaacctcaga ttccaactgc tgcttatacc atacaacctc ttatccctta
      841 ccgtggcagc atgcccatgg aagaattaag atacaaaacc gatttctatt ccaagaatca
      901 taagcgtccg gcagtatttc tcctgctcat tgggaatgtg gccatgcgac aggcaagagc
      961 ccaattttcg agtaattttt tcggatgtgc cggctttcaa atcataacgg gcaatcccgt
     1021 agcgagtgtt gacgaaggca ttcgagctgc ccatgaaagt a
//
LOCUS       DAORPD010000762         1061 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_30384_length_1061_cov_1.781312, whole genome shotgun sequence.
ACCESSION   DAORPD010000762 DAORPD010000000
VERSION     DAORPD010000762.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30384_length_1061_cov_1.781312"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..568
                     /locus_tag="PK976_08390"
     CDS             56..568
                     /locus_tag="PK976_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HRC67947.1"
                     /translation="MKPKCENDLYPILDQFGIQYHIYRHPPAPTIQIAREFWKDIPSS
                     HCKNIFLRNHKGNQHYLVVLEHTREFSIHSLEKRLHQGKLTFASAKRLMQYLGVTPGN
                     VSPYALINDVEHHVHLFLDGTLLSAKSISFHPLTNTASLVTSFEDFLRFLNWTQNTYE
                     FINLTEDYHE"
     gene            561..>1061
                     /locus_tag="PK976_08395"
     CDS             561..>1061
                     /locus_tag="PK976_08395"
                     /inference="COORDINATES: protein motif:HMM:NF015039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HRC67948.1"
                     /translation="MNKKLWIGILLLIPSLLFSQKPVDELRYQSITPKFYRKILADTG
                     LRMTPSSVAFARMVAITQKSMPTNIDLYQRLWHQPSLSQGESGTCWCFAAVSFLESEC
                     NRLTGKEIKLSEMFFVYHEYLERAAQFVKTRGETDFGEGSETSSIPILLKRYGCMPES
                     AYPGKPP"
BASE COUNT          311 a          235 c          188 g          327 t
ORIGIN      
        1 atttaatttc tcgagaaaag attaattttg aaagctattg gaatttcatt caagaatgaa
       61 gcctaaatgt gaaaatgatc tttatcccat tctcgaccaa tttggaattc aataccatat
      121 ttaccggcat cctcctgctc ctaccataca gattgctcgt gaattctgga aagacattcc
      181 cagttctcat tgcaaaaaca tcttcctgag aaatcataag gggaatcagc actatctggt
      241 tgttttggaa catacccggg aattttcgat acactctctc gaaaaacgct tgcatcaggg
      301 aaaacttacc tttgcctcag ccaaacgact gatgcagtac ctgggcgtta cccctggcaa
      361 cgtttctcct tatgccttaa tcaatgatgt ggaacatcat gttcatcttt ttttagatgg
      421 aactctcctt tcggctaaaa gcattagctt tcatccgctc accaatacag cttcactggt
      481 tacttctttc gaagattttc tgcgattttt aaactggact caaaacacct atgaatttat
      541 caacctaacc gaagattatc atgaataaga aattatggat aggtatctta ttgcttatcc
      601 catccttgtt attttcacag aaacccgtcg atgaattgag atatcaaagc atcaccccta
      661 aattttaccg aaaaatacta gccgatacag gtttgaggat gactccttca tctgtggcct
      721 ttgcacgtat ggtggctatt acacaaaaat ccatgcccac aaacattgat ttgtatcaga
      781 ggttatggca ccaaccttct ctaagccaag gcgaatcggg aacatgttgg tgttttgcag
      841 cagtatcttt tcttgaatca gaatgtaatc ggttgacagg caaagaaatt aagctttcgg
      901 aaatgttttt cgtttatcat gaatatctcg aacgagccgc tcagttcgta aaaacacgtg
      961 gagaaactga ttttggtgaa ggctctgaaa catccagtat tcccattctt ctgaaacgtt
     1021 atggctgcat gcctgaatcg gcttaccctg gaaaacctcc t
//
LOCUS       DAORPD010000763         1060 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_30411_length_1060_cov_2.921393, whole genome shotgun sequence.
ACCESSION   DAORPD010000763 DAORPD010000000
VERSION     DAORPD010000763.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30411_length_1060_cov_2.921393"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(563..>1060)
                     /locus_tag="PK976_08400"
     CDS             complement(563..>1060)
                     /locus_tag="PK976_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67949.1"
                     /translation="FTSKDTLWVKFEFADSLKRKALVKYPLDYNSSYQLIIPSKTFVD
                     IFGLANDSITARFTTRAADAYGSFHLKLISPASNNQYLIQLLDEKENLVRQDIINDLV
                     CEIDYGMLLPKKFILKAVLDCNRNRKWDTGNYRKKRQPEKVQYFKAPIEIRANWDLRE
                     EWILE"
BASE COUNT          343 a          198 c          209 g          310 t
ORIGIN      
        1 ccggatttcc gattcttatg gggatgttcc tgaaggggaa attgtagctc tattttcgag
       61 tacagggctt ttgcagattg ccattaatca tggaaacgcc tcaggattgc ttggccttag
      121 cattgacgac agcatcagaa tcgttttcga ataagtccgt cgtaaatgtt accgcttctg
      181 agttgcggga gaaatggtaa tagtaaattg aaaggtaaaa gcgaaaaaaa taagataaaa
      241 aaatggaatg agatacaggg taccaataga caagatgaac ggaaaattga gaggcaagaa
      301 tacaggaaaa tgaagcgaca ttttcatagt aattcatcat tcgcaaattt cgggttatcc
      361 caaattatct atgaagaact gcaaaagaaa tcgtttcgat ctatcctgac aaacagttgg
      421 caaatgggaa agaaaagtgg ccgagaaggg cttctccctt ctcggccacc aaaaataaaa
      481 tttcatgttt catgctattt ccgaacactt cttccctctt tgcaataatc tatctcgata
      541 aaaaaaccag gcaagaactg cattattcca aaatccattc ttcacgtaaa tcccaattgg
      601 cccttatttc tattggtgcc ttaaagtatt gtactttttc gggttgacgt ttttttctgt
      661 aatttcctgt atcccatttc ctatttcgat tgcaatccaa gacagctttc aaaataaatt
      721 tttttggcag caacattcca tagtctattt cacaaactaa atcattgata atatcctggc
      781 ggacaaggtt ttctttttca tcgagcaatt gaataaggta ttgattattg ctggcaggag
      841 aaatcaactt caggtgaaaa ctcccataag catcggcagc acgcgtagta aagcgtgcgg
      901 taatgctatc gttggccagt ccgaaaatat ccacaaaagt cttggaagga ataatcaact
      961 gataagaaga attataatcg agaggatatt tgaccaaagc cttgcgtttg aggctatcgg
     1021 caaattcgaa tttaacccat aaggtatctt tgcttgtgaa
//
LOCUS       DAORPD010000764         1060 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_30424_length_1060_cov_2.067662, whole genome shotgun sequence.
ACCESSION   DAORPD010000764 DAORPD010000000
VERSION     DAORPD010000764.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30424_length_1060_cov_2.067662"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="PK976_08405"
     CDS             complement(<1..478)
                     /locus_tag="PK976_08405"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HRC67950.1"
                     /translation="MDIIEQTFDPLDMNQYKIEKLPKREKNKTEKFLSFIGPPLALIV
                     FLLLAFVLKPSFLQSFDPEKLTKGAKEVFDRVGSKVFYRNNCYMLAIFAASLILWITG
                     AIPNYLTSLIVIISIVLTGVLPEKEAFAQLGHPVMWLNIMSFVLASMLVSTGVAKRF"
     gene            complement(598..>1060)
                     /locus_tag="PK976_08410"
     CDS             complement(598..>1060)
                     /locus_tag="PK976_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008767136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HRC67951.1"
                     /translation="QEIEKKFLVKGDFKPFAHQATRITQGYLSSVPERTVRVRIKGNK
                     GYITVKGIGNESGASRFEWEMEIPLEDAEQLMKICEPGVIDKTRYLIKAGQHTYEVDE
                     FYEENQGLVLAEVELQREDEPFEKPEWLGEEVTGDPRYFNSMLMKNPYSCW"
BASE COUNT          311 a          239 c          204 g          306 t
ORIGIN      
        1 caaacctctt ggcaaccccc gtacttacaa gcatactggc caagacaaac gacatgatgt
       61 tcagccacat cacaggatga cccaactgcg caaaagcctc cttttcgggc aaaaccccag
      121 taagaactat agaaatgatg actatgagcg aagtaaggta atttggaata gctccagtaa
      181 tccataaaat aagactggca gcaaaaatag ccagcatata gcagttgttc ctgtaaaaaa
      241 ccttactccc caccctatcg aaaacttcct tagcaccttt tgtgagtttc tccgggtcga
      301 agctttgaag aaatgagggc ttcaacacga aagccaataa taggaaaacg atcaaagcca
      361 aaggtggacc tataaacgat aaaaattttt cggtcttatt tttttcacgt ttggggagtt
      421 tttcgatctt atactgattc atatcgagcg gatcgaaagt ctgctctatg atatccattt
      481 ctgagtgatt aagaattcaa cgaatcaatg aatacatttt aaaaacttag ataaaagtga
      541 aagatccagt ccgaagagac attcctctcc ggactggatc ttcgagggtg gagtggttta
      601 ccagcacgaa taaggatttt tcatcagcat ggaattgaag taacgagggt cgccggttac
      661 ttcttcgcct aaccattcgg gcttttcgaa gggttcgtct tcacgctgca attcaacctc
      721 agctaaaacc aatccttggt tttcttcata aaactcatct acctcatagg tatgctgccc
      781 agccttgatt agatagcggg ttttatctat taccccaggt tcgcatatct tcatcaattg
      841 ctcggcatcc tcgagtggaa tttccatttc ccactcaaaa cggctagctc ctgattcatt
      901 gccaattcct tttacggtga tatagccttt gtttcctttg atcctcactc gtactgttcg
      961 ctcaggtact gaagaaagat aaccctgagt gatacgggta gcttgatgag caaagggttt
     1021 aaaatcacct tttacaagga attttttttc gatttcttgt
//
LOCUS       DAORPD010000765         1059 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_30474_length_1059_cov_2.158367, whole genome shotgun sequence.
ACCESSION   DAORPD010000765 DAORPD010000000
VERSION     DAORPD010000765.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30474_length_1059_cov_2.158367"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            444..>1059
                     /locus_tag="PK976_08415"
     CDS             444..>1059
                     /locus_tag="PK976_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67952.1"
                     /translation="MNSKLLFVLATSLVPLIGVQAQSIDQAKVIPSTYDRSSLTCLYM
                     KFPGENHVTEIASKFQQVVFSDKYYNNNLANVIFEAPYSRTNTEIVPEVAIKDYLTNQ
                     KIAKSVISKWYNRKEDGTMTMDLIFERGMFNASDAEYIKAQTTKRGNALLQDYGNRLI
                     QRSYILVFDFANVKNMSEAKVADQHGWEATVTAYLYKIDFNEEIQ"
BASE COUNT          365 a          199 c          187 g          308 t
ORIGIN      
        1 gctctctcgc tcccttttta atgttaggtc gagaccatta tcatcaatac aagggagcag
       61 tgaaaggact caaaatagat ttctcaaaaa aattattcta tagcccggaa agtggataac
      121 caaacttcga aatataattt ctatttttac cttgcttttg aaaattcaac ttttcgtatt
      181 tcgaaacaga gtcggagagt atagattttt aaggagagga tgaaaaattt tctactgata
      241 ttttgaaatt taataaagct gacttcttta cgaaaaaaac aatggaatga aattcagttt
      301 attagcaaaa atagaatcgc tttctcttga tagattacag gatatgattc ttcagaagtt
      361 tttccagatt ttatctttca ttcgaaaaat tttgttcttt taccaaaaat ttcaacatca
      421 atccaccaaa accttaatga actatgaatt caaagctatt attcgtgctg gccacatcat
      481 tggtgccatt aattggcgtt caagcccaga gtatcgatca ggccaaagta atcccttcga
      541 cttacgacag aagctctctt acctgcctgt acatgaaatt tccaggcgag aaccatgtaa
      601 cagaaatcgc gtcaaaattc caacaggtcg tgttttctga taaatattac aacaacaacc
      661 ttgcgaatgt aatctttgag gcgccttatt cgagaaccaa caccgaaatt gttcccgaag
      721 tagcaatcaa ggattatctg acaaatcaaa agatagccaa gtcggttatc agcaaatggt
      781 ataatagaaa agaagatggt accatgacga tggaccttat ctttgaaaga ggcatgttta
      841 atgcgagcga tgccgaatac atcaaggcac aaaccacaaa acgtggtaat gccttattgc
      901 aagattatgg caacagactt attcagcgca gttatatctt agtattcgac tttgccaatg
      961 taaaaaacat gagcgaagcc aaagtagccg atcaacatgg ctgggaggcc actgttacgg
     1021 cttatttata caagatcgat ttcaatgaag aaattcaag
//
LOCUS       DAORPD010000766         1059 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_30475_length_1059_cov_2.107570, whole genome shotgun sequence.
ACCESSION   DAORPD010000766 DAORPD010000000
VERSION     DAORPD010000766.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30475_length_1059_cov_2.107570"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1059)
                     /locus_tag="PK976_08420"
     CDS             complement(<1..>1059)
                     /locus_tag="PK976_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HRC67953.1"
                     /translation="FNALKEVSIRKPVELDTPGINISDMFATNVFDLPKMEKYLPREA
                     FKSVKTSIETGVPITRQVADEVATGLKAWAIENGATHYTHWFHPLTDATAEKHDAFLE
                     RTNGTNMIETFSGELLIQQEPDASSFPSGGIRNTFEARGYTAWDVSSPAFIVGTTLCI
                     PTIFVSYTGEALDYKTPLLKALTALDKAAVKVCRYFNKSVTKVIATLGWEQEYFLIDE
                     ALYYARPDLMLADRTLMGHQSSKDQQLSDHYFGSIPERVMAFMRDFELEAYKLGIPIK
                     TRHNEVAPNQFECAPIYEEINIAVDHNQLLMDVMRRTARRHGFRVLFHEKPFAGINGS
                     GKHNNWSIETNTGVNLLSP"
BASE COUNT          301 a          220 c          211 g          327 t
ORIGIN      
        1 ggtgaaagca agttcacacc tgtatttgtt tctattgacc aattattatg tttaccagag
       61 ccgttaatac ctgcaaaggg tttttcgtgg aaaagtactc taaaaccatg tcggcgtgca
      121 gtacgccgca ttacatccat aagcaactga ttatggtcaa cagcaatatt aatttcctca
      181 taaattggcg cacattcgaa ttgatttggt gcaacctcgt tatgccgcgt ttttattggg
      241 attccaagtt tataggcttc gagctcaaaa tctctcataa atgccatcac tctttcgggt
      301 atagagccaa aataatggtc ggaaagttgc tggtctttcg atgactggtg ccccattaaa
      361 gtacggtcgg ccagcatcag gtcgggccgg gcataataaa gtgcctcgtc aatcaaaaaa
      421 tattcctgct cccatccgag tgttgcgatt acttttgtaa cactcttatt aaaataccgg
      481 catactttta cagcagcttt atcaagtgct gtcagagctt ttaataaagg agttttataa
      541 tcaagagctt ctccggtata agaaacaaaa atagtaggaa tacatagcgt ggttcctaca
      601 ataaaagcag gagaagaaac atcccatgcc gtgtaacctc tggcttcgaa tgtatttctt
      661 atacctccac ttggaaagct tgaagcatca ggctcttgct gaataagaag ctcacctgag
      721 aatgtttcta tcatattagt accgttcgtc cgttccagaa aagcatcatg tttttctgct
      781 gtagcatcgg taagcggatg aaaccaatga gtatagtgtg tcgcaccatt ttcaattgcc
      841 catgccttga ggcctgtagc tacttcgtcg gctacctgcc ttgtaatggg aacacctgtt
      901 tcaatcgatg tctttacgga tttaaatgct tcacgtggta aatatttttc cattttggga
      961 agatcgaaaa cattcgttgc aaacatatcg gaaatattaa tacctggagt gtcaagttct
     1021 acaggctttc ttatggaaac ttcttttaat gcattaaat
//
LOCUS       DAORPD010000767         1059 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_30476_length_1059_cov_2.105578, whole genome shotgun sequence.
ACCESSION   DAORPD010000767 DAORPD010000000
VERSION     DAORPD010000767.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30476_length_1059_cov_2.105578"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            144..>1059
                     /gene="murG"
                     /locus_tag="PK976_08425"
     CDS             144..>1059
                     /gene="murG"
                     /locus_tag="PK976_08425"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HRC67954.1"
                     /translation="MHGGSTGMNILISGGGTGGHIFPAIAIAKALQKLRPDADILFVG
                     AKGKMEMEKVPQAGFIIKGLWISGFSRSFSISNLLFPLKLMVSSISAYYILRRFRPVV
                     VIGVGGFASGPVMRVASYLKIPIVIQEQNSYPGVTNRILAKKASRIFTAYPNMEAFFP
                     AEKTLLTGNPVRKEIINTEHKRPEAIAYFELKEKVTTILVIGGSQGALSINEAVKTCL
                     SLRHTQWMWQTGRIYFEKAFAFINENHLENVKTRAFIERMDLAYAAADIIISRAGAIA
                     ISELCIIGKPTILVPYPYAAANHQMHNAR"
BASE COUNT          323 a          194 c          251 g          291 t
ORIGIN      
        1 gtcagtatgg gaggcacttc tatctggttc acgtgcatca tgtttggaat gttgcaaagt
       61 attgccagag gcgataaaaa gatagatatc gtacaggaag aaaaacttgt agaaaatacg
      121 gaagagaaaa tagaagaaaa tatttgcatg gaggaagtac tggcatgaat atcctgataa
      181 gtggtggagg aactggaggt catatttttc cggccattgc aattgcaaag gctctgcaaa
      241 agctaaggcc tgatgccgac attctttttg ttggggcaaa aggcaaaatg gaaatggaaa
      301 aagtgcctca agcaggtttc atcatcaaag gattgtggat tagtggtttc agccgttcat
      361 tttcgatttc taacttgctt ttccctttga aactaatggt aagcagcata agtgcttact
      421 atattcttcg aaggtttcgt ccggtcgtag tgataggtgt tggaggattt gcaagtggac
      481 cagtcatgcg agtcgcttct tatcttaaga ttcccattgt aattcaagaa caaaattcct
      541 atccaggggt aactaataga atacttgcga agaaagcctc acgaattttc acggcttatc
      601 ccaatatgga agcatttttc ccggctgaaa aaaccttgct tactggtaat cctgtacgca
      661 aggaaattat taataccgaa cataagcgac ctgaagccat tgcttatttc gaactgaaag
      721 agaaggttac aacgatttta gtgattggag gaagtcaggg tgccttatcc atcaatgagg
      781 cagttaaaac ctgtctttcg ctgagacata cccagtggat gtggcaaacg ggcagaattt
      841 atttcgagaa ggccttcgcg tttataaacg aaaaccatct ggagaatgta aagacaagag
      901 cctttataga aaggatggat ctagcctatg cagcggctga cattattatt tcgagggctg
      961 gtgccatagc tataagcgaa ttatgtatca ttgggaagcc gactatactg gttccgtatc
     1021 catacgctgc agccaatcat cagatgcata atgcccgcc
//
LOCUS       DAORPD010000768         1059 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_30479_length_1059_cov_1.937251, whole genome shotgun sequence.
ACCESSION   DAORPD010000768 DAORPD010000000
VERSION     DAORPD010000768.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30479_length_1059_cov_1.937251"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            123..>1059
                     /locus_tag="PK976_08430"
     CDS             123..>1059
                     /locus_tag="PK976_08430"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HRC67955.1"
                     /translation="MNLIYLDYNATTPLATEVIEAMKPFMEVYFGNPSSAYVLGQKTR
                     LAVEEARRQVSTLLGCQPDEVIFTSGGTESNNMAIKGAALANRSKGNHIITSCIEHPS
                     VLEVCHWLESHGFRITYLPVDEFGRVDLDAFRAAISEETIIVSIMHSNNETGSLQPVQ
                     EIGMIAKQHGILFHVDAAQSIGKIPVKVDDFCVDLLSVAGHKFYAPKGIGALYIRRGV
                     KIEKIIHGASQEFGWRPGTENVIHIAGLGMACKLVNERLENDHVHLKSLRDRLQAGLF
                     QRIDGVKLNGHPELRLPNTLNVSIAGIEANALISEL"
BASE COUNT          319 a          190 c          254 g          296 t
ORIGIN      
        1 cattattcga aacttaatgt cttagattca gatttcctga tttggcttaa aatgcaaagg
       61 taaaattatt taactatcga tagataatat tttcgagact gtctaatttt gccgaaaaaa
      121 cgatgaatct catttatctg gattataatg ccactacacc tttggccaca gaagtaatcg
      181 aggccatgaa accatttatg gaggtttatt ttggaaaccc ttccagtgct tatgttctgg
      241 ggcaaaaaac caggttggcc gtcgaagaag cgcgaagaca agtttcgact ttattgggat
      301 gccaacccga tgaagtcatt tttaccagcg gtggaaccga gtcgaacaac atggctatca
      361 aaggtgcagc cttagccaat cgttcgaaag gcaatcatat cattacttcc tgtatcgagc
      421 acccatcggt gcttgaagtc tgccattggc tcgaaagtca tggattcagg attacctatt
      481 tacccgtaga tgagtttggc cgggtggatt tagatgcatt cagggctgct ataagcgaag
      541 aaaccattat cgtgagcatt atgcattcca acaatgagac aggtagtctg caacccgtgc
      601 aggaaatcgg aatgatagca aagcagcacg ggattttatt ccatgtagat gccgctcaaa
      661 gcatagggaa aatacctgtt aaggtggatg atttttgcgt tgatttattg tcggtagccg
      721 ggcataagtt ttatgcacca aagggaatag gggctttata cattcgaaga ggagtaaaga
      781 tagaaaaaat catacatggt gcatctcaag agtttggatg gaggccaggg acggaaaatg
      841 tgatacatat tgctggattg gggatggcct gtaaactggt taacgagcga ttggaaaatg
      901 atcatgttca tctgaaatca ttgagagatc gtttgcaagc tggattattt cagagaatcg
      961 atggcgtcaa actgaatgga catcccgaac tcaggcttcc caatactttg aatgttagta
     1021 ttgcaggtat agaagcgaat gctttgattt cggaactga
//
LOCUS       DAORPD010000769         1056 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_30630_length_1056_cov_2.296703, whole genome shotgun sequence.
ACCESSION   DAORPD010000769 DAORPD010000000
VERSION     DAORPD010000769.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30630_length_1056_cov_2.296703"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..>1056)
                     /locus_tag="PK976_08435"
     CDS             complement(2..>1056)
                     /locus_tag="PK976_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67956.1"
                     /translation="CYKFVSKINSPKTLPQAMKTNFFTKIVVSAFLLIIIAYGCTTTT
                     YLIVSEGESFNALTKITDNENPCFDSDGGSEGTNLVFTVREKDGSYNIYMKDNVLSRA
                     TIQKTYGTNLNVSPAVCVAKGKIAFQYYDKNNFDIYYVDAYKGKAITQVTYTDENEYN
                     PSWNPEGTIIIFEKGAQPRSFIAVSEKNNKAAIYNLVTVTKNQIWLKNLVTGELKMIG
                     EGSFPSISPDGNMIAFIKYDLNKQKNAEVGTLWIMTIDGDSPRQITTSDLGYAIRPKW
                     SPDGTQIVFQLTKKNKTDSDIYTIDVNGENLKQHTNNPADDFSPFWSSDGYIYFSSDR
                     GAKKGEYQIWRFKIEE"
BASE COUNT          309 a          188 c          187 g          372 t
ORIGIN      
        1 gttattcttc tattttgaat ctccatattt gatactcacc ttttttagcg ccacggtcgg
       61 aactaaaata aatatatcca tcacttgacc agaaaggaga aaaatcgtca gcagggttat
      121 tagtatgttg ttttaaattt tcaccattta catctatggt ataaatgtcg gagtcggttt
      181 tgtttttctt ggtgagttgg aagacaattt gcgttccatc ggggctccat ttaggccgaa
      241 tagcatatcc caaatctgaa gtagtaattt ggcgaggaga atctccatca atagtcatga
      301 tccacaaagt tcccacttct gcattctttt gtttgttcaa atcgtattta atgaatgcaa
      361 tcatgtttcc atcgggtgat attgaaggga aacttccttc acctatcatt ttcaattctc
      421 ctgttacgag atttttaagc caaatctggt tttttgtaac agtaaccaag ttataaatgg
      481 cagctttatt attcttttca gatactgcta taaaagaacg tggttgtgcc cccttttcaa
      541 aaataatgat tgtaccttca ggattccacg aaggattgta ctcattttca tcagtatagg
      601 taacctgcgt aatggctttg cctttataag catctacata gtaaatatcg aaattattct
      661 tatcgtagta ctgaaaagct attttccctt tagctacaca aactgctggc gatacgttta
      721 ggtttgttcc gtaagttttt tgaatggtgg ctctcgacaa aacattatct ttcatgtaaa
      781 tgttataaga accatccttt tctctaacgg taaaaaccaa attagttcct tcacttcccc
      841 cgtcagaatc aaaacagggg ttttcgttgt cagtgatttt cgtcagggca ttgaaagatt
      901 ctccttcgct aacgattaaa taggttgtag tggtacatcc ataagcaata attatcagga
      961 gaaaagcaga aacaacgatt ttagtaaaaa agtttgtttt catggcttgg ggtaatgttt
     1021 ttggtgaatt gattttagaa acaaatttat aacaat
//
LOCUS       DAORPD010000770         1056 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_30641_length_1056_cov_1.994006, whole genome shotgun sequence.
ACCESSION   DAORPD010000770 DAORPD010000000
VERSION     DAORPD010000770.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30641_length_1056_cov_1.994006"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="PK976_08440"
     CDS             complement(<1..508)
                     /locus_tag="PK976_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007900567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HRC67957.1"
                     /translation="MDNTDKQAKLKALEATISNIEKNYGKGAIMRLGDQPTEAIPVIS
                     TGSIGLDTALGVGGLPKGRVIEIFGPESSGKTTLALHAIAEAQKQGGIAAFIDAEHAF
                     DRTYAQKLGVDTDNLLVSQPDYGEQALEISDNLIRSNAIDIIVIDSVAALTPKSEIEG
                     EMGDSKMGL"
BASE COUNT          291 a          217 c          208 g          340 t
ORIGIN      
        1 gaaggcccat ttttgaatct cccatttcgc cttctatttc gcttttaggc gtaagggccg
       61 ctacggagtc gatcacgatg atatctatgg cattgcttct gatgaggtta tcgctgattt
      121 cgagggcttg ttctccataa tcgggctgtg aaaccagaag gttatcggta tctaccccta
      181 gtttttgagc atacgtacgg tcgaaagcat gctcggcatc tataaaagca gctatacctc
      241 cctgtttttg agcttcagca atagcatgaa gcgccagcgt ggttttacct gaagattcag
      301 gaccaaatat ttctattact ctccccttgg gtaaaccccc aacaccaaga gcagtatcca
      361 ggccaatgga acccgtcgag ataacaggaa tggcctcggt gggctgatcc cccaaacgca
      421 taatggctcc cttgccataa ttcttttcaa tattactgat agtggcttcc agagccttta
      481 attttgcttg tttatcggta ttatccatga tgatgtgatt gaattatttg agtaattttc
      541 ttcttatcgt ttttccctat aagtccatca aaggtaaaat aaatttcgat aatcgaacta
      601 caataaaaac ccgagtcatt aaaatttagc ttacactgag aaatgtcata ttctccccgt
      661 tggattttct aaaaaagact ttcaaacagt atacagcgat aaattgactg ctatggaaga
      721 ttcgacggct attttcatta ctaagtcgtc taatcattac taccactcag gaccaggccg
      781 tcttcgaaag tcaggataat atcgtcaccg gatgtgtcat agaataactg tctttgttgc
      841 taatctccat gaagattggg cttcttgtcg atatgaggaa tgtaatagct gtcagccagg
      901 cctttaatgt aagaggatga tatgtctcga agcaacgacc tttttttgct tggaatttct
      961 attcaaaaat gggaaagatt agctttttgt ccatatagca atattttaaa aatctttttc
     1021 aagaatctgt gagctgtgat gtttagtatc gatttg
//
LOCUS       DAORPD010000771         1055 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_30679_length_1055_cov_3.141000, whole genome shotgun sequence.
ACCESSION   DAORPD010000771 DAORPD010000000
VERSION     DAORPD010000771.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30679_length_1055_cov_3.141000"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="PK976_08445"
     CDS             <1..516
                     /locus_tag="PK976_08445"
                     /inference="COORDINATES: protein motif:HMM:NF019903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRC67958.1"
                     /translation="PDFETRVAILQKKIYNEGLVIPEDVVEYIAANITTNVRELEGAL
                     TSVVAQSALNKKAISLELARKIIDKFVICTTKEISMDYIQKVVCDYFNISIEQLKSKS
                     RKRSIVQARQLVMYFAKEYTTSSLAAIGLQCGNKDHSTVLHACRTVKNLFETDRTFKQ
                     YYTDIERKIKI"
     gene            528..>1055
                     /locus_tag="PK976_08450"
     CDS             528..>1055
                     /locus_tag="PK976_08450"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRC67959.1"
                     /translation="MLLESIPQPVDIYEAHKRISPYIHRTPVITSKTLDELSQCNLFF
                     KCENFQKAGAFKSRGACNAVFSLDDDESAGGVATHSSGNHAQALARAALLRGIKSYIV
                     MPRTAPIVKIAAVKHYQGKITFCEPTLAAREETLKNLLQQTNAIEIHPYNNYSVIAGQ
                     ATATMEIFEEIGWPDF"
BASE COUNT          332 a          235 c          211 g          277 t
ORIGIN      
        1 cctgattttg aaacacgtgt ggcaatactt cagaaaaaaa tatataatga aggccttgta
       61 atacccgaag atgtagtcga atacattgcc gccaacatta ccaccaatgt gcgtgaactc
      121 gagggagctc ttacatcggt tgttgcccag tcggcactca ataaaaaggc gatttcgctc
      181 gagttggcac gaaaaatcat cgataaattc gtgatttgca ctaccaagga aatctctatg
      241 gattacattc aaaaagtggt atgcgattat ttcaatatca gtatcgaaca gctcaagagc
      301 aagtcacgca agcgctccat cgttcaggca cgccagcttg tcatgtattt tgcaaaggaa
      361 tatacaacat cttctttagc agctattggg cttcaatgtg gcaacaaaga ccactcgact
      421 gtattgcatg cctgccgaac tgtaaaaaat ctgtttgaaa ccgatagaac ctttaaacaa
      481 tattatactg atattgaaag gaaaataaag atttaaaccg aaaccgtatg cttctcgaaa
      541 gcattcctca acctgttgat atatatgaag ctcacaagcg tatttctccg tacattcacc
      601 gaacacctgt aattaccagc aaaactctgg atgaactaag ccaatgtaat ctttttttca
      661 aatgcgagaa ttttcagaag gcaggggcgt ttaaatcgcg gggagcgtgc aatgctgttt
      721 tttcgctcga tgatgatgaa agtgcagggg gagtagcaac ccattcgtcg gggaatcacg
      781 ctcaggccct tgccagagcc gcattgcttc gcggaataaa atcctatatc gttatgccac
      841 gtacggcccc aatagttaaa attgctgccg taaaacatta tcagggcaaa ataaccttct
      901 gtgaacccac tcttgctgct cgtgaagaaa ccttgaaaaa cttgcttcaa caaaccaatg
      961 ccatcgaaat ccatccctac aacaattata gtgttattgc tgggcaagcc accgctacaa
     1021 tggaaatttt cgaagaaata ggttggcctg acttt
//
LOCUS       DAORPD010000772         1054 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_30734_length_1054_cov_2.087087, whole genome shotgun sequence.
ACCESSION   DAORPD010000772 DAORPD010000000
VERSION     DAORPD010000772.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30734_length_1054_cov_2.087087"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1054
                     /locus_tag="PK976_08455"
     CDS             <1..>1054
                     /locus_tag="PK976_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67960.1"
                     /translation="FNYWNPDFFVDTHTTDGADYQYVLTYSLETGPNTDSSLGKYLRE
                     NYIPTMEAYMSEKGYKVFPYVNFKQWHDPRSALYSLPASAMLSHGYVAARNRPALLIE
                     THMLKPYKLRVSSTLELLFFTLKHLNDHYRDYLQINRQADTYTSSADFRPFPLKFNFI
                     TNNTDSVMVDFEGYDYRIEKSDLTGGNWFIYLPRPKTFRLPNFNHAIAQDSILLPDYY
                     IIPPEWTTIIERLRLHGIEMNFLTYDTILKVESYRFSNPQWANFPFEGRNRLSVKTEK
                     YVERRAYPAGSALISLQQPTARIIALLLEPQSSDSFLTWGFFNAIFEQKEYAETYVME
                     KIARQMIEKRPDLLPQ"
BASE COUNT          308 a          261 c          188 g          297 t
ORIGIN      
        1 tattcaacta ttggaatccc gattttttcg tcgatacaca cactaccgat ggtgccgact
       61 atcaatatgt cttgacctat tcacttgaaa ccggacccaa taccgattct tctctgggta
      121 aatatctccg ggaaaattat attccaacta tggaagccta catgagcgaa aaaggataca
      181 aagtctttcc ttatgtgaat ttcaaacaat ggcatgatcc acgctcggcg ttatactctt
      241 tgccggcttc ggctatgtta tcgcacggat atgtcgcagc ccgcaatcgc cccgctctgc
      301 tcatcgaaac ccacatgctg aaaccctata aattacgagt atcgagcacc ctcgaactct
      361 tattttttac attgaaacat ctgaacgatc actatcgcga ctatttgcaa ataaaccgcc
      421 aagccgatac gtatacatct tcggccgatt tccgtccgtt tcctctgaaa ttcaatttta
      481 taaccaacaa taccgatagt gtgatggtag atttcgaagg ttatgactac agaattgaaa
      541 agagtgattt aaccggtgga aactggttta tctacttacc tcgacctaaa acatttcgct
      601 tgccaaattt caaccatgcc atagctcagg attctatact gctgcccgat tattacatca
      661 ttccgcccga gtggactaca atcatcgaaa gacttcggct tcatggaatt gaaatgaatt
      721 ttttaaccta cgacacaatt cttaaagttg aatcatatcg cttcagcaat cctcaatggg
      781 caaacttccc attcgaaggg cggaacaggc tttctgtaaa aacggaaaaa tatgtcgaaa
      841 gacgagctta cccggctggc agcgcactga ttagtcttca gcagccgact gctcgcatta
      901 tagctctctt gctcgaaccc caatcttccg attcattcct tacctggggt ttcttcaatg
      961 ccattttcga acagaaagaa tacgccgaaa cttatgttat ggaaaaaata gccaggcaaa
     1021 tgattgaaaa acgacctgac ttattgcctc agtt
//
LOCUS       DAORPD010000773         1051 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_30895_length_1051_cov_1.743976, whole genome shotgun sequence.
ACCESSION   DAORPD010000773 DAORPD010000000
VERSION     DAORPD010000773.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30895_length_1051_cov_1.743976"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1051
                     /locus_tag="PK976_08460"
     CDS             <1..>1051
                     /locus_tag="PK976_08460"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HRC67961.1"
                     /translation="AARIAAIEATAEGLNWTFAPMVDIARDPRWGRISEGAGEDTYLG
                     MAMAKARVKGFQDNDLSSPYTMMACAKHYAAYGAAQAGRDYNTVDVSDRTLREVYLPP
                     FKACVDQGVATFMTSFNEIAGVPCTGSKYLLTDILRKEWGFEGFVVTDYTSINEMIPH
                     GFAADEKHAGELALNAGVDMDMQGAIYYNYLKKSLDEGKVKMADIDAAVKRILIMKFK
                     LGLFDDPYLYCNKEREQQDIMRPEHLTFAREMAKKSIVLLKNEQNLLPLSKNIGTIAV
                     IGPVANNRRELIGSWSAAGDWTKAITLLEGIRQSISPETKILYAKGCSITDDSTQYIQ
                     EAINLAKKAEVVILAV"
BASE COUNT          318 a          252 c          236 g          245 t
ORIGIN      
        1 gagccgcacg aattgctgcc atcgaggcta cagccgaagg cctcaactgg acttttgctc
       61 caatggtgga tattgctcgc gatccacgtt ggggaagaat atcagaaggt gccggtgaag
      121 atacttacct cggaatggct atggccaaag ccagagtgaa gggattccag gataacgact
      181 tgtccagccc ttatacaatg atggcctgtg caaaacatta tgccgcttat ggggcagctc
      241 aggccggccg ggattataat actgtcgatg tctccgaccg taccctgcgc gaagtctatc
      301 tgcctccctt caaagcctgc gttgaccaag gtgtggcaac cttcatgact tcattcaacg
      361 aaattgccgg agttccttgc actggcagca aatatctact caccgacatc ctgcgaaaag
      421 aatggggctt cgaaggattc gtcgtaaccg attatacttc catcaatgaa atgatcccac
      481 atggttttgc tgccgacgaa aaacatgccg gtgaacttgc ccttaatgct ggcgtagata
      541 tggacatgca aggagccatt tattacaact acctgaaaaa atcactcgac gaaggaaaag
      601 tgaaaatggc agacatcgat gcagccgtaa aaagaatcct tatcatgaaa ttcaaattag
      661 gccttttcga tgatccctat ctatattgca ataaagaacg tgaacaacaa gatattatga
      721 ggcccgaaca tcttaccttt gcaagagaaa tggcaaaaaa atccattgtt cttctaaaga
      781 atgaacaaaa tctcttaccc ctgtcgaaga acattggcac tatcgccgta ataggccctg
      841 tagccaataa tcgtcgtgaa ctcattgggt cgtggtcagc ggcaggagac tggacaaaag
      901 ccattacgtt gcttgaagga attcgtcaga gcatttcacc cgagacgaaa attctctatg
      961 ccaaaggatg tagcatcaca gacgattcca cccagtacat acaagaagcc atcaacctgg
     1021 ccaaaaaagc cgaagtagta attcttgcag t
//
LOCUS       DAORPD010000774         1050 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_30930_length_1050_cov_2.223116, whole genome shotgun sequence.
ACCESSION   DAORPD010000774 DAORPD010000000
VERSION     DAORPD010000774.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30930_length_1050_cov_2.223116"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            5..>1050
                     /locus_tag="PK976_08465"
     CDS             5..>1050
                     /locus_tag="PK976_08465"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HRC67962.1"
                     /translation="MTTYLEKNSNRIMEELFGLLKIPSISSLSEHKDDMYRAAEYYKN
                     ALLKAGMDKVEIFDTPGHPVVYAEKILSPDFPTVLVYAHADVMPVDPIELWQSPPFEP
                     EIREGKIYARGADDDKGQGFMHVKAIEYLVNSRQLQCNVKFMIEGEEEIGSPHLEDFC
                     QEHKDMLKADIILLSDTSMIAPDTPSITVGLRGLAYIEVEVAGPNRDLHSGLYGGIVH
                     NPIHVLSRMIGNLHDDHGRIIIPGFYDDVRELSEEERIEFNLAPFDEESYKKSLGINC
                     LWGESGFTPLERIGIRPSLDVCGIWGGYTGEGSKTILPSKASAKVSMRLVPDQDPKKI
                     ANLFENYLHEMVPNT"
BASE COUNT          324 a          216 c          234 g          276 t
ORIGIN      
        1 agatattacc acatacctcg aaaaaaattc aaaccgaatc atggaagaat tattcgggct
       61 tctgaagatt ccatccatca gcagcctgag cgaacataaa gatgatatgt atcgcgcagc
      121 cgagtattac aaaaatgctc ttttgaaagc cggcatggat aaagtcgaaa tcttcgacac
      181 acctggccat ccggtagttt atgcggaaaa aattttatca ccggattttc caactgtgct
      241 ggtatatgcc catgccgatg tcatgccagt tgatcccatc gagttatggc aatcacctcc
      301 tttcgagcct gaaatcaggg aagggaagat ttatgcacga ggtgccgatg acgacaaagg
      361 acagggattc atgcacgtaa aagccataga ataccttgta aattcccgtc aattgcagtg
      421 caatgtaaaa tttatgatag agggtgaaga agaaattggt tccccgcatt tagaagactt
      481 ttgccaagaa cataaagaca tgcttaaggc agatatcata ttgctttccg ataccagcat
      541 gattgctccg gatactcctt ctattacagt tggattaaga ggccttgctt acatagaagt
      601 tgaagttgcg gggccaaatc gtgatttgca ttccggactt tatggaggaa tcgtacacaa
      661 tcccattcat gttttatcca gaatgatagg caaccttcac gacgaccatg gccgtataat
      721 cattccggga ttttacgacg atgtaaggga actgagcgaa gaagaacgaa tagagttcaa
      781 tcttgctcct ttcgatgagg aatcttacaa aaaatcattg ggaattaact gcctctgggg
      841 tgaaagtgga tttacacctc tggagcgtat aggaattagg ccctctcttg atgtttgtgg
      901 aatatgggga ggatatacag gcgaaggatc gaaaactata ttaccgtcga aggcctcggc
      961 taaagtctcc atgcgtctgg tgcctgatca ggatcccaaa aaaatcgcca atttattcga
     1021 aaactattta catgaaatgg ttcccaatac
//
LOCUS       DAORPD010000775         1049 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_30999_length_1049_cov_1.754527, whole genome shotgun sequence.
ACCESSION   DAORPD010000775 DAORPD010000000
VERSION     DAORPD010000775.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30999_length_1049_cov_1.754527"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(290..>1049)
                     /locus_tag="PK976_08470"
     CDS             complement(290..>1049)
                     /locus_tag="PK976_08470"
                     /inference="COORDINATES: protein motif:HMM:NF025930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67963.1"
                     /translation="IELPCVNKSRVVTSIEGNTIYIGFIHIENLERDFSQLIVSERER
                     NGLYLDLADFIERTQAKLEQTLLLIRTGALRFTGKTKANLMWEAHIFLNKKNSQASSQ
                     PPLFKISARPFQLPALPSNSLEDAYDEIELLGFPVTVSYFDMLQTRFRGEVISRNMIQ
                     HVGKTVRMLGYLVTLKYVRTVKNELMHFGTFVDAEGELFETVHFPNSLKQYPFHGKAM
                     YLLAGTITEEFQHPSLTITKMAKLPFQPDPRHKN"
BASE COUNT          293 a          180 c          261 g          315 t
ORIGIN      
        1 tgtccttaga tgacaaataa actgattctt cctcaatcag ttagttgtgc gaaaatttta
       61 tgtataatta atcctgttag gggattcgat agtggcaggt agatgaccta atgatattat
      121 tgaaacaccg tcaagagata tgaaatgttg ggaaggtcag gttttagtta taacacatag
      181 ttctattgtt aagcgaagaa agtagctgcg aaaaatcatg aagatacagc ttgccagggt
      241 ggttcgaccc aggcggatgc tgaagcgaac ccgggtttcc ttcgatagtt taatttttat
      301 gtcgaggatc gggctgaaaa ggcaattttg ccattttagt aatggtgagc gagggatgtt
      361 gaaattcttc ggtaatggtt cctgcgagca gatacatggc ctttccatga aagggatatt
      421 gcttgagcga gttggggaag tggacggttt cgaagagttc accctcggca tctacaaagg
      481 ttccaaaatg catcaattcg tttttaacgg tacgtacata tttgagtgta actaaatatc
      541 cgagcattct tacggttttg ccgacatgct gtatcatgtt tcggcttatt acttctccac
      601 gaaaacgggt ttgtagcata tcgaaataag acacggtaac ggggaaacca agaagttcta
      661 tttcgtcgta ggcatcttca agcgaattgg aaggaagagc aggaagctgg aaaggtcgtg
      721 cagaaatttt gaataaggga ggttgagaag aagcctgaga atttttttta ttcaggaaga
      781 tatgtgcttc ccacatcaga ttggctttcg tttttccggt aaatctaaga gctcctgtgc
      841 ggattagcag aagggtttgc tccagtttgg cctgagtgcg ttcgataaaa tcggccaggt
      901 caagataaag gccatttctt tctctttcgc ttacgataag ttgcgaaaaa tcacgttcga
      961 ggttttcgat atggatgaat ccaatgtaaa tggtgtttcc ctctatcgag gtaaccactc
     1021 gactcttgtt tacgcagggc agttcaatt
//
LOCUS       DAORPD010000776         1046 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_31164_length_1046_cov_2.029263, whole genome shotgun sequence.
ACCESSION   DAORPD010000776 DAORPD010000000
VERSION     DAORPD010000776.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31164_length_1046_cov_2.029263"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            83..205
                     /locus_tag="PK976_08475"
     CDS             83..205
                     /locus_tag="PK976_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67964.1"
                     /translation="MPSIVEMIDEVISHIDDDSVIEKVGHKVKEMMSAYPLFAY"
     gene            complement(285..>1046)
                     /gene="selD"
                     /locus_tag="PK976_08480"
     CDS             complement(285..>1046)
                     /gene="selD"
                     /locus_tag="PK976_08480"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HRC67965.1"
                     /translation="MGAKPLFALNIVAFPSHRLPLAVLENILKGATDKAAEAGIPIMG
                     GHTIDDAELKFGLAVTGTIHPNKIMRNCGAKPGDRIILTKPIGSGILSTALKQGLLQK
                     STEEIFLKNLLTLNKKASEIMLSFPVTACTDVTGFGLLGHLMEMCKPGHLGAILYRRA
                     IPLLPEVEIFAMAGIVPGGSKGNLEYVKNDVIFTEDIPLPFQYIYSDAQTNGGLLIAV
                     MSEEAENLLESLHEAGIHQASLIGEFTDGKKIEII"
BASE COUNT          300 a          216 c          205 g          325 t
ORIGIN      
        1 ttcaagtttg aaatgttcag ctttgccagc gtctggcgta acttggacga tgttataatg
       61 tccatcttcc caagttgaga caatgccttc gattgtcgaa atgatagatg aagttatctc
      121 tcatatcgac gatgattctg ttatcgaaaa ggtcggccat aaggttaaag aaatgatgag
      181 cgcttaccct ttattcgctt attgatgctt gcgtaatcgc tgtatgaaga gactctcggt
      241 gataagctaa gattgaaaca gaagaactca gagaaaatgc gaattcatat aatttcgatt
      301 ttcttaccat cggtaaattc tcctataagg cttgcctgat gaatgccagc ttcatgcaaa
      361 gattctaata gattttcagc ctcttcgctc ataacagcaa tcaacaatcc tccgttggtt
      421 tgagcatcgg aataaatata ctgaaacggc aagggaatat cttctgtaaa aatgacatca
      481 ttctttacat attcaagatt tcccttcgat cctccgggaa ctattcctgc cattgcaaaa
      541 atttctacct caggcaaaag aggtattgct cttctataca atatagcacc cagatgaccg
      601 ggtttgcaca tttccatcag gtgtcccagc aggccaaatc cagttacatc agtacatgct
      661 gttacaggga aggaaagcat aatttcggag gctttcttat tcaatgtaag taaattttta
      721 aggaaaatct cttcggtaga tttttgtaat aatccttgtt ttaaagcggt tgacagtatt
      781 cctgaaccta ttggtttggt caagataatt cgatcaccag gtttagctcc acaatttctc
      841 ataatcttat tgggatgaat cgttcctgtt actgccagtc caaatttgag ttcggcatca
      901 tctattgtat gcccccccat aataggtatg cctgcttcgg ccgccttatc ggttgctcct
      961 ttgaggatat tctcgagcac tgcgagaggt aaacgatgag atggaaaagc aactatattc
     1021 aatgcaaaaa gcggtttggc tcccat
//
LOCUS       DAORPD010000777         1046 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_31165_length_1046_cov_2.024218, whole genome shotgun sequence.
ACCESSION   DAORPD010000777 DAORPD010000000
VERSION     DAORPD010000777.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31165_length_1046_cov_2.024218"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1022)
                     /locus_tag="PK976_08485"
     CDS             complement(<1..1022)
                     /locus_tag="PK976_08485"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRC67966.1"
                     /translation="MNKILKPLGLYFAPETSTSNRCMLGGMVSNNSCGAHSLIYGSTR
                     DHLLEIKAMLSDGSEVEFKDLSPAEFQAKLSGNQLENKLYRQIYHILSDPENQEEIRK
                     EYPDPSVRRRNTGYAIDSLLECQPFKPDGAPFNFCRLIAGSEGTLALITEIKLSLVPL
                     PPPVSGLVCIHLENVKDALYANLIALKYHPVSVELMDKTVLDLTHENREQDKNRFFVQ
                     GNPGAILIVEFYENTLSEMEVKVEAMKTEMIQAGYGYAFPLITGKDIQRVWALRKAGL
                     GVLSNMPGDAKPVPVIEDTAVNPELLPAYIEEFDRILQRLNLSCVYYAHIGSGELHLR
                     PVLNLK"
BASE COUNT          302 a          228 c          208 g          308 t
ORIGIN      
        1 tcttttaggt tcaaaacagg tcttagatgt agttcaccac tgccgatatg tgcataataa
       61 acacaggata aattcaaacg ctggaggatt cgatcgaatt cctcgatata agctggaagt
      121 aattcgggat ttactgcggt atcttctatg acaggaacgg gtttagcatc gccgggcata
      181 ttcgataaaa ctcccagccc ggcttttcgc aaagcccaaa cgcgttgaat atcctttccc
      241 gtgatgagtg gaaaagcata accataccca gcctgtatca tctccgtttt catggcctca
      301 acttttactt ccatctccga gagagtattt tcataaaatt ctacaattag aatagcccca
      361 ggattacctt gcacaaaaaa acgattcttg tcctgctcac ggttttcatg ggttaaatcc
      421 aaaaccgttt tatccattag ttcaaccgaa acgggatgat atttcagtgc aatgagattt
      481 gcatataatg cgtcttttac attttctaaa tgaatgcaaa caagccctga aactggaggc
      541 ggcaagggaa ccaaacttag cttaatttcc gttattagag caagtgttcc ttccgagcct
      601 gctattaaac ggcagaagtt gaatggagca ccatcgggtt taaatggttg acactcgagc
      661 aaagagtcga tggcataacc tgtatttcga cgacgaacac taggatcggg atattctttt
      721 cggatttctt cctggttttc aggatcggat aaaatatgat aaatttgacg atataattta
      781 ttttcgagct gattgccact tagtttggcc tggaattccg caggagaaag atctttgaat
      841 tccacttcgc taccatcgct caacatagcc tttatctcca gcaaatggtc tcttgtgctg
      901 ccatagatta atgaatgagc accacatgaa ttgttactca ccatgcctcc aagcatgcaa
      961 cgattagaag ttgaggtttc tggagcaaaa tataatccta aaggcttcaa tattttgttc
     1021 atctcatcga gtatcacgcc gggctc
//
LOCUS       DAORPD010000778         1046 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_31177_length_1046_cov_1.606458, whole genome shotgun sequence.
ACCESSION   DAORPD010000778 DAORPD010000000
VERSION     DAORPD010000778.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31177_length_1046_cov_1.606458"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(73..927)
                     /locus_tag="PK976_08490"
     CDS             complement(73..927)
                     /locus_tag="PK976_08490"
                     /inference="COORDINATES: protein motif:HMM:NF025034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HRC67967.1"
                     /translation="MKRIEIPFQIFKILEQGYHIAIAVKVNGMPAHWIIDSGASHSVF
                     DKARISCFLPEFQLKESPMMAMGLGNELEPFLLKVSDFEIEKRKISKYQATLLDLSPL
                     NQIYSRYFNDPIHGVLGCDLLVKLKAKLNYKKKTIELKKEKKPMQILSIMPDSEHLMV
                     TLKVRKQKANMLIDTGASSTIFDLNLFKQFYPYEASQLERTDQPSAGIANDAQNFGAA
                     LIPELCIGPVKMTNYEMLLIDLEHINSLYSKLDLPPIDGLLGNDLLFKLKASLSFKSH
                     SLRLPFPH"
BASE COUNT          312 a          176 c          254 g          304 t
ORIGIN      
        1 ttcaacatag aattcttacg agacaagcca aaactcgaca aaatgatttt gcggggaggg
       61 catgggagaa aatcagtgag ggaatggcaa tcgaagtgaa tgagatttga agctaagtga
      121 ggctttcaat ttgaacagca ggtcattgcc cagtaggcca tcaataggag gcaagtccaa
      181 tttggaatat aatgaattaa tatgttcgag atcgataagc agcatttcgt aattcgtcat
      241 ttttaccggg ccaatgcaaa gttcagggat cagagcagca ccaaagtttt gagcatcatt
      301 agcaatccca gcggagggct ggtcggttcg ctcgagctgg ctggcttcat aaggatagaa
      361 ctgtttgaaa aggtttaaat cgaaaatggt tgaagaagct ccggtatcga ttagcatatt
      421 tgccttttgt tttcgaacct tcagtgtaac catcagatgt tcgctgtcgg gcataatgct
      481 gagaatttgc attggttttt tttctttttt gagttcgata gttttctttt tgtaattcaa
      541 ttttgccttt agcttaacca gcaggtcgca gccaagtact ccatgaattg gatcattgaa
      601 ataccgggaa taaatttggt tgagaggtga gagatcgagg agtgtagcct gatatttcga
      661 aatttttcgt ttttctattt cgaaatcgga aactttcagt aaaaagggtt ccagctcatt
      721 gccgaggccc atagccatca tgggggactc tttcagttga aattccggta aaaaacatga
      781 gattctcgct ttgtcgaaaa ccgaatgaga ggctccagaa tcgatgatcc aatgagcggg
      841 cataccattt acttttacag cgatagcaat atgataacct tgttcgagga ttttaaaaat
      901 ctggaaaggg atttcgattc gtttcatgat aagggattaa tagatgaagc tgctgccgcc
      961 caagaattcg cgaagaatgc ttgtaggagg aatttcacgg gaagaaattg gttgaaaata
     1021 agaaaggaat ttcataagtc tcaggg
//
LOCUS       DAORPD010000779         1046 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_31193_length_1046_cov_1.407669, whole genome shotgun sequence.
ACCESSION   DAORPD010000779 DAORPD010000000
VERSION     DAORPD010000779.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31193_length_1046_cov_1.407669"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="PK976_08495"
     CDS             complement(<1..470)
                     /locus_tag="PK976_08495"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HRC67968.1"
                     /translation="MLITGIIGQDDKEKTANIINSILHNSQKRISILDSKNLSSLNAK
                     QVKSYLAELERNNIDILILKIDLTDIMSEIYDFLRFDIIVFTDKADEINGEMEQNYLE
                     LMKRAFSLLNEKGIAIVNADDNDLSKFFKDIKHYIVTYGFNLKASMTTSSIGDLV"
     gene            complement(599..>1046)
                     /locus_tag="PK976_08500"
     CDS             complement(599..>1046)
                     /locus_tag="PK976_08500"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRC67969.1"
                     /translation="DVANHSYSHFRMGSIDNGKIRTEISKCDSVLEKLTGKKVDLFRA
                     PYGDYNNNVVRIARELNHFTIQWDVDSLDWRPGIKPEEIKSRILKNVKNGSILLFHND
                     TPHTAKILPDIITALKDRGYGFIPVSELIMRENYEIDHTGRQKRKD"
BASE COUNT          296 a          204 c          152 g          394 t
ORIGIN      
        1 accaaatcgc ctatgcttga tgtggtcata ctggccttga gattaaaacc gtaagttaca
       61 atatagtgct taatatcctt aaaaaactta ctaagatcat tatcatcggc atttactatt
      121 gctatacctt tttcgtttaa taaggaaaaa gctcttttca ttaattccaa ataattttgt
      181 tccatttctc cattaatttc atcggcttta tcggtaaaaa caataatatc aaatcttaaa
      241 aaatcataaa tttcgctcat gatgtcagtt aaatcaatct ttaatataag tatatcgata
      301 ttatttctct caagctcagc cagataactc ttaacctgct ttgcattcag gctggacaag
      361 ttctttgagt caagaatgct gattcttttt tgagaattgt gcaatatcga attaattata
      421 ttagctgttt tttccttgtc atcttgcccg attatacctg ttatcagcac cgattcaacc
      481 ccatttcttt tgatagctta agtttataat gtaccctttt tatgaatttt atgtagagga
      541 attataacac tttagttcaa agcaaatttt tcgtttatag tataattttc atgaatcttc
      601 aatcttttct tttttgtctt ccggtatggt caatttcgta attctctctc ataataagtt
      661 ccgatacggg aataaatcca tagcctctgt ctttaagagc agttattatg tcggggagta
      721 tttttgcagt atggggcgta tcattgtgaa acagcaagat tgacccgttt tttacatttt
      781 tcaatatgcg gctctttatc tcttcgggtt tgatgcccgg ccgccagtcc aaagaatcca
      841 catcccattg aatggtaaaa tggtttaatt ccctcgcaat tcttacaaca ttgttgttat
      901 aatccccata gggtgctctg aacaaatcga ccttttttcc tgttaatttc tccaaaacgc
      961 tgtcgcattt cgatatttcc gttctaatct taccattgtc aatactcccc attctaaaat
     1021 gcgagtaaga atggtttgca acatcg
//
LOCUS       DAORPD010000780         1043 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_31344_length_1043_cov_1.802632, whole genome shotgun sequence.
ACCESSION   DAORPD010000780 DAORPD010000000
VERSION     DAORPD010000780.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31344_length_1043_cov_1.802632"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..141
                     /locus_tag="PK976_08505"
     CDS             <1..141
                     /locus_tag="PK976_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004338653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HRC67970.1"
                     /translation="RHYPVMEYIQPEIFSELKKIALEKGFKAAECGPLVRSSYHASEY
                     KI"
     gene            143..832
                     /gene="lipB"
                     /locus_tag="PK976_08510"
     CDS             143..832
                     /gene="lipB"
                     /locus_tag="PK976_08510"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HRC67971.1"
                     /translation="MTLHHKTLCIDLGLAEYKQSWDYQEQMCRQLQKAVLNGSYANAC
                     NYLLFVEHPHVYTLGKNGKPGNLLVNSKFLNKIGASFCMTNRGGDITYHGPGQIVVYP
                     LFYLEAFRMGVRQYIENLEEAVIRMLNDYQIKAMLMPGAIGVWLDADTPAARKICAIG
                     VRVSRGISMHGLALNVNTRLEYFNYIHPCGFVDKGVTSMEKELHSNLNIHEVKENLRG
                     KLAKAFDLEFV"
BASE COUNT          319 a          174 c          230 g          320 t
ORIGIN      
        1 cggcattatc ctgtgatgga atatattcaa cctgagattt ttagtgagct aaaaaaaatt
       61 gctctcgaaa agggattcaa ggctgctgaa tgtggacctt tggtgcgttc atcctatcat
      121 gcatctgagt ataaaatttg aaatgacact gcatcacaaa acactttgta tcgatctggg
      181 attggctgaa tataaacaaa gctgggacta tcaggaacaa atgtgccgcc aattgcaaaa
      241 ggctgtttta aatggttcgt atgccaatgc ttgcaattac ttgctgtttg ttgaacatcc
      301 ccatgtttat acactcggga aaaatggaaa accaggcaat ttgctggtaa atagtaaatt
      361 tttaaacaaa atcggagcaa gtttttgtat gaccaatagg ggaggggata ttacatatca
      421 tggaccgggt caaattgtgg tttacccttt attttacctc gaagcctttc ggatgggtgt
      481 gaggcagtat atcgaaaatc tcgaagaagc cgtgatccgt atgctgaatg attaccaaat
      541 aaaagccatg cttatgcctg gagctattgg tgtatggctg gatgctgata cacccgctgc
      601 acgtaaaatc tgtgcaatag gggttagggt tagcagagga attagcatgc atgggctggc
      661 acttaatgta aatactcgac tcgaatattt taactacatt catccttgcg gatttgtaga
      721 taagggtgtg acctccatgg aaaaagaact ccattccaat cttaatatcc acgaagtgaa
      781 agaaaatcta agaggaaaac ttgccaaggc ctttgacctg gaatttgttt gaagtttctt
      841 tattactcga aaattttaaa cttaccgcga tatagtggaa gttttttagc tcgaaccttg
      901 tctgcttgtg tcgctcgatc gaatgacgat tgatcggatt attttatcag gagtaaacaa
      961 atcgaaatga agtattttat tggttttgaa atatttatgc taaaataaat ggatttgaag
     1021 tctttctgtg aacaagcagt tgt
//
LOCUS       DAORPD010000781         1042 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_31374_length_1042_cov_2.678825, whole genome shotgun sequence.
ACCESSION   DAORPD010000781 DAORPD010000000
VERSION     DAORPD010000781.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31374_length_1042_cov_2.678825"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="PK976_08515"
     CDS             <1..537
                     /locus_tag="PK976_08515"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HRC67972.1"
                     /translation="DFIQSLSNVNDGSAMLLGPVLDPLRSSSILKNLHTLHIRMQKHS
                     SNAMYLARKFRETGLKTIYPGFEDDPSYPVFNKIMNPDYGFGGMLAVDLETSEKAAAF
                     MEKMELAGVGYLAVSLGYFKTLFSNSGKSTSSEVPPEYQQKMGLSEGLVRFSVGLDHD
                     IAETWSKIETCLKELRII"
     gene            712..>1042
                     /locus_tag="PK976_08520"
     CDS             712..>1042
                     /locus_tag="PK976_08520"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006044284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HRC67973.1"
                     /translation="MLKVGDKAPDFLGIDQNENTISLKDYEGKKVVLYFYPKANTPGC
                     TAEACNLNDNLERLKAKGYEIIGVSADSVKTQKSFAEKYHLNFPLIADTDKNIIKSYG
                     AWGKKKMY"
BASE COUNT          334 a          210 c          209 g          289 t
ORIGIN      
        1 gattttattc aatccctgag caatgtaaac gacggaagtg ccatgcttct cggccctgtt
       61 ctcgatccct tacgctcatc ttctattttg aaaaacctcc atacattgca tatccgtatg
      121 caaaaacata gcagcaatgc catgtatctt gcccgaaaat ttcgtgaaac cggtttgaaa
      181 accatctatc ccggcttcga agacgatccc agctatccgg tattcaataa aatcatgaat
      241 cctgattatg gttttggagg tatgctggcc gtcgatttag aaaccagcga aaaagctgct
      301 gcttttatgg aaaagatgga attggccggg gtgggatatc tggccgtgag cttgggatat
      361 tttaaaactc ttttcagtaa ttcagggaaa agtacatcga gtgaagtccc tccagaatat
      421 caacaaaaaa tggggctctc cgaaggctta gttcgcttct cggtgggcct cgatcacgac
      481 attgcagaaa cctggtcgaa aatcgaaacc tgcctcaaag aacttcgaat catctgagat
      541 gtatttgccc tatgcggttt cagtttttat atggaaagtt cataatcgca ttttcaacgc
      601 atttcacccc atgagcaatg actttttgaa caaataagga tcaaattatg ttattttcag
      661 taaaagtcat aatatttcat ccaaattcaa ccatttaaaa tcaaaatagt tatgctaaaa
      721 gttggagata aagccccgga ttttttgggc atagatcaaa atgagaatac aatttcctta
      781 aaagattacg aaggaaagaa agtagtgctt tacttttacc ccaaagccaa tactcctggt
      841 tgtacagccg aggcctgcaa tctgaacgac aatttagaac gattaaaagc caaaggttat
      901 gagattattg gcgtaagtgc cgatagcgtg aaaacccaaa agagttttgc cgaaaaatat
      961 catctgaact tccctttgat agctgatacc gacaagaata tcatcaaaag ttatggggca
     1021 tggggcaaaa agaagatgta cg
//
LOCUS       DAORPD010000782         1041 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_31416_length_1041_cov_2.835700, whole genome shotgun sequence.
ACCESSION   DAORPD010000782 DAORPD010000000
VERSION     DAORPD010000782.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31416_length_1041_cov_2.835700"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="PK976_08525"
     CDS             <1..468
                     /locus_tag="PK976_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRC67974.1"
                     /translation="ALGFGMKTYAFRRTPMDEIIKAEGIIPLSKAEDIYKNCQYVTLH
                     IPANEQTKKSINYDLLSLMPKGATLVNTARKEIIDEESLLKMFAERPDFKYLSDIEPA
                     CKAEIIEKYSKRYFFTPKKMGAQTLEANNNAGIAAAQQIIDFFEKGDVRFKVN"
     gene            747..>1041
                     /locus_tag="PK976_08530"
     CDS             747..>1041
                     /locus_tag="PK976_08530"
                     /inference="COORDINATES: protein motif:HMM:NF017451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HRC67975.1"
                     /translation="MTVFRFQDLRVYQKTLEFAEWASRIAEGHSSNGQGQIISNKLLD
                     SAVGIVFCIAEGSASPRPVFIEQLKKAKSHVRECIVMSELMLSLKLLNERNYEY"
BASE COUNT          346 a          214 c          196 g          285 t
ORIGIN      
        1 gctttaggct ttggaatgaa aacctatgct ttccgacgta cccctatgga cgaaatcata
       61 aaagcagaag gaatcattcc cttatcaaaa gctgaagaca tttacaaaaa ctgtcagtat
      121 gttacccttc atatcccggc caacgagcaa acaaaaaaat caatcaatta tgatttgcta
      181 tcgctaatgc caaaaggagc cactttagtt aacacagccc gcaaagaaat catcgacgaa
      241 gaaagccttt tgaaaatgtt tgccgaaagg ccagatttca agtacctaag tgatattgag
      301 cccgcctgca aggctgagat catcgagaaa tactccaaac gttacttttt tactccaaaa
      361 aagatgggtg ctcaaaccct cgaagccaac aataatgcag gcatagccgc tgctcaacaa
      421 attatcgatt tcttcgaaaa gggtgacgtt cgctttaagg taaattgatc cattccttca
      481 ttacatcata acaaaactaa agccgttctt tgaaattggg taaaggtatt ccccgtattt
      541 tacggatgaa agttctataa atcctttcaa atctgcatca tttactaccg aaaggttttg
      601 atacaataaa acagaactct cataaggaag tgcctttaat catataacga caacatgaat
      661 tgaaatcctg aagccaaagg tttggaatca ggaagttatc ctcgacagta tttcatagta
      721 ttcacttcat ttaagaattt acaccaatga cagtttttag gtttcaagat ttgagagttt
      781 atcaaaaaac gctggagttc gcagagtggg cttcacggat tgccgaagga cattcatcca
      841 atgggcaagg gcaaatcatc agcaacaagc tacttgattc ggcagtgggt atagtttttt
      901 gcatagccga gggttcggct tcgccccgac ctgttttcat cgaacaattg aaaaaggcaa
      961 agtctcatgt gagagaatgt atcgttatgt cggaactcat gttatctctt aaactgctaa
     1021 atgaacgcaa ttacgagtat t
//
LOCUS       DAORPD010000783         1041 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_31418_length_1041_cov_2.663286, whole genome shotgun sequence.
ACCESSION   DAORPD010000783 DAORPD010000000
VERSION     DAORPD010000783.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31418_length_1041_cov_2.663286"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..961
                     /locus_tag="PK976_08535"
     CDS             <1..961
                     /locus_tag="PK976_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67976.1"
                     /translation="TLSLLLAGIQLRAQTGEAKATVQIDSTRLMIGQQVRMNISFTHP
                     PKAKIGWGIRGDTLSRQIEIIKKSAIDSTVTPDGMVKRSQNLVITSFDTGYIVVQPIR
                     FYWQLPQDTITQIAETEPILLEVMAMPVDTTQDIRDIKGIQKIGYSWKEILPWIGYAL
                     LIAIVAFLIYRYVQYRRGKRKYFLIAPKPKIPAWQIALEALDTLRNKQLWQSGHYKQF
                     YSELTDIFRDYLDNQFQINAPEMVSEEIIIALENAGFDTKLISDCRRMLQTADLVKFA
                     RYIPLPDENSFSFDVCRRFVEETMPKPENASEPHPSSPSEMQQ"
BASE COUNT          330 a          215 c          201 g          295 t
ORIGIN      
        1 aacattatcg ctcttgcttg ctgggataca attaagagct caaacaggcg aggccaaagc
       61 aactgtccaa attgattcaa ctcgcttgat gattggccaa caggtgagaa tgaatatcag
      121 ttttacccat ccacctaagg ctaagatagg atggggaatt agaggagata cactaagtcg
      181 ccaaatcgaa attattaaaa aatctgctat tgattcgaca gttacacccg atggaatggt
      241 taaaagaagc cagaatcttg tcattacatc ttttgatacg ggatacattg tagtacaacc
      301 cattagattc tactggcaac ttccccagga cacaattacg caaatagccg aaaccgaacc
      361 aattttgctg gaggtaatgg ctatgccagt cgatacgact caagacatcc gcgacattaa
      421 aggcatacag aaaatcggat atagttggaa agaaatcttg ccatggatag ggtatgccct
      481 cttgattgct attgtggcat ttctaattta ccgctatgtt cagtaccgaa gaggcaagcg
      541 aaaatatttc ctcattgctc ctaagcccaa aattccggct tggcaaattg cgctggaagc
      601 attagatacg ctcagaaaca aacaattatg gcagtcaggt cattataagc aattctattc
      661 tgaacttaca gatatcttta gggattatct ggacaatcaa tttcaaataa atgctcccga
      721 aatggttagc gaagaaatta tcatagcttt agaaaatgcg ggctttgata cgaaattgat
      781 cagtgattgc cgaagaatgt tgcaaaccgc tgatctggtt aaatttgcac gttacatacc
      841 tttacccgat gagaattcat tttcatttga tgtttgtcga aggtttgtgg aagaaaccat
      901 gcccaaacct gaaaatgctt cggaacccca tccttcttct ccatctgaaa tgcaacaata
      961 aaccactatg attggattca ttcataatat cacttttgcg catccgtggt tgttactctt
     1021 attattgact attcccctac t
//
LOCUS       DAORPD010000784         1041 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_31428_length_1041_cov_2.384381, whole genome shotgun sequence.
ACCESSION   DAORPD010000784 DAORPD010000000
VERSION     DAORPD010000784.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31428_length_1041_cov_2.384381"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1034)
                     /locus_tag="PK976_08540"
     CDS             complement(<1..1034)
                     /locus_tag="PK976_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRC67977.1"
                     /translation="MAEKFSRRDVLKVLGLTLGGTLLPKISWPRDEPIYPLILPEEPA
                     VPLSKPITAITLGAGSRGNVYGNYALYYPEQLKIIGVAEPIPIRNERYSKLHHITDEN
                     RFVTWEQVFERPKFADAVLITTPDRLHYGPAMAALKMGYDLLLEKPIAPSEKECLDIL
                     ELTEKTGQIVAVCHVLRYSPYFIKLKELVQKKAIGELISIQHMEPIEHIHMSHSYVRG
                     NWHNSKESTPIILAKSCHDLDILRWIVVKPSKRIQAFGDLSWFKKENAPRGATPRCMD
                     GCAIERNCPYSALKIYYRERGWLYVFDLPDEKEKEGDKRKKILVVDDEEFIGEMFALV
                     SERIGVDVVY"
BASE COUNT          286 a          242 c          213 g          300 t
ORIGIN      
        1 caataaacca catctacccc tatcctctct gataccaagg caaacatctc tcctataaac
       61 tcctcgtcat caacaacgag tatctttttt ctcttatctc cttctttttc cttttcgtcg
      121 ggtaaatcga atacataaag ccagcctcgt tcacggtagt aaatcttcag ggcagaataa
      181 ggacaattgc gttcgatggc gcatccatcc atacagcgcg gagttgcacc ccggggagca
      241 ttttcttttt taaaccaact aagatcgcca aatgcttgaa tacgtttaga aggcttaacc
      301 acgatccatc gtaaaatatc gagatcgtga catgatttgg caagaataat aggcgtagat
      361 tctttcgaat tatgccagtt acctcttaca tacgaatgac tcatgtgaat atgttcgatg
      421 ggttccatgt gctgaatgct gatcaattcc ccaatggcct tcttctgcac aagctctttt
      481 aatttgatga aatagggaga atatctcaaa acatgacaaa cagcaacaat ttgacccgtt
      541 ttttcggtga gttccaaaat gtccaaacat tctttttccg aaggagctat gggtttctcc
      601 aagagcaaat catagcccat tttcagggcg gccatggccg gaccataatg caatcgatcg
      661 ggggttgtaa tgaggacggc atcggcaaat tttggacgct cgaatacctg ctcccaggtt
      721 acaaaccggt tttcgtcggt aatatgatgc agtttgctgt atctttcgtt acgaatggga
      781 atgggttcgg ccacgccaat aatcttcaac tgttcaggat aataaagggc ataattgcca
      841 tacacattcc cccgtgaacc tgctccaagg gttatagccg taatgggttt ggaaagaggc
      901 actgcgggtt cttcaggcag gataagcgga taaatcggct cgtcccttgg ccatgaaatt
      961 ttaggtagta aggtcccgcc taaggtgaga cccaatactt tcaaaacatc tcttctcgaa
     1021 aatttttctg ccatgttatt t
//
LOCUS       DAORPD010000785         1040 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_31502_length_1040_cov_2.024365, whole genome shotgun sequence.
ACCESSION   DAORPD010000785 DAORPD010000000
VERSION     DAORPD010000785.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31502_length_1040_cov_2.024365"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..>1040
                     /locus_tag="PK976_08545"
     CDS             149..>1040
                     /locus_tag="PK976_08545"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HRC67978.1"
                     /translation="MNKFSKFFLIFAGMSFVLASCTKDLDTVPIDKDVVTSAQVYENP
                     KAYKQILAKIYAGLAVSGQQGPSGMPDISGIDEGFGQYLRGYWYHQELTTDEAIIGWN
                     DQTIKDFHWQTWGAGDVFISAFYYRIFYQISLCNEFIRETTDAKLDERNVTGSLRTDI
                     EHYRAEAQFMRALSYYHALDMFGTVPFVTEKDPVGSFFPPQISRKDLFDYIEKELLEI
                     EPLMIDARQNEYGRADKAAVWMLLAKLYLNSEVYTGVKHYDEVVTYTKKIIDAGYTLE
                     PNFEHLFMADNDRSNEVIFSI"
BASE COUNT          294 a          217 c          212 g          317 t
ORIGIN      
        1 ctacggtaaa taacgtgttt accattacca attacaaagg tctcgatcct gaaatattca
       61 gcgggattga ctatagcatg tatcctcgta ccagggtatt cgttctgggt cttaatttaa
      121 tattttaacc gataaaaaaa cagctaatat gaataagttc agtaaattct ttttaatttt
      181 tgcaggtatg agctttgttt tggcctcctg caccaaggac ctcgatacag ttccaattga
      241 caaggatgtt gttacttcag cccaggtata tgaaaatcct aaagcctaca agcagattct
      301 tgctaaaatt tatgccggct tggctgtctc cggtcaacag ggcccttcgg gcatgcccga
      361 tattagtggt attgacgaag gatttggcca atatctgcgt gggtattggt atcaccagga
      421 actcactacc gatgaagcca ttattggatg gaacgaccag acaataaagg atttccattg
      481 gcagacatgg ggtgccggcg atgtttttat ctccgccttc tactatcgta tattctatca
      541 aatttctctt tgtaatgaat ttatcagaga aacaaccgat gctaaactcg atgaacgcaa
      601 tgttactggc agccttcgta ccgatatcga acattatcgt gctgaagccc agtttatgcg
      661 tgctttgagc tattatcatg ctctcgatat gtttgggaca gttccttttg tgaccgaaaa
      721 agaccctgta ggctcattct tcccacctca aattagccgt aaagatcttt tcgattacat
      781 cgaaaaggaa ttgcttgaga tagaaccttt gatgatagat gctcgccaaa atgaatatgg
      841 acgcgccgat aaagccgctg tttggatgct tttggctaaa ttgtacctca actctgaagt
      901 atatactggc gtgaaacatt acgatgaggt agtaacctat accaaaaaaa tcatcgatgc
      961 gggttatacc ctcgagccaa attttgaaca tctctttatg gctgataatg atcgttccaa
     1021 cgaggtcatt ttctcgatca
//
LOCUS       DAORPD010000786         1038 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_31609_length_1038_cov_2.238047, whole genome shotgun sequence.
ACCESSION   DAORPD010000786 DAORPD010000000
VERSION     DAORPD010000786.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31609_length_1038_cov_2.238047"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="PK976_08550"
     CDS             complement(<1..542)
                     /locus_tag="PK976_08550"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HRC67979.1"
                     /translation="MMIPTQDIHSYVSALSSIFEEEGNRETASGAKAYMRNQFEFYGL
                     PSPLRRRLQKGFLLQNGYLSIEQWPELIFQCWNSAYREMQYFAMEYSQHFIPHCTEEH
                     IVLLEWMMTNKAWWDTVDFISPKLCGKFFIHFPDIIENTTSKWIDSQNLWLQRAALIF
                     QLNYKEKTHLDLLFRSIQCL"
     gene            complement(539..907)
                     /locus_tag="PK976_08555"
     CDS             complement(539..907)
                     /locus_tag="PK976_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HRC67980.1"
                     /translation="MMAEHNEMGKEGEELAAEYLVKQGYTIEARNWRYGHEEIDIIAR
                     KDDLLLIVEVKTRRSNYFGQPESFVSFAKQKALIRCANRYIYQHNFEGDTRFDIISII
                     MTPKGNQINHIPNAFYPTLR"
BASE COUNT          301 a          204 c          210 g          323 t
ORIGIN      
        1 cttaaacact gaatggatcg aaacaataaa tcaagatggg ttttttcttt gtaatttaat
       61 tgaaaaatca gtgcagctcg ctgtagccat agattttgac tatcaatcca ttttgaagtt
      121 gtattttcga tgatatccgg aaaatgaata aaaaattttc cgcaaagttt aggactgata
      181 aaatctactg tatcccacca tgccttgttc gtcatcatcc attcgagcag tacaatatgt
      241 tcttcggtgc aatgaggaat aaaatgttga gaatactcca ttgcaaaata ttgcatttca
      301 cgataggcag agttccaaca ctgaaatatg agttcaggcc attgctcgat ggagagatat
      361 ccattttgta gaaggaagcc tttttgaagc cgacgacgca ggggtgaagg aagtccgtaa
      421 aactcgaact gatttcgcat ataagctttg gcgccgcttg ccgtttctct attgccctcc
      481 tcttcgaata tggatgatag agccgaaaca taagaatgaa tgtcttgagt cggaatcatc
      541 atcttaaggt tggataaaag gcattgggaa tatgattgat ttgatttccc ttgggtgtca
      601 taatgatgga aataatatcg aaacgggtat caccttcgaa gttgtgttga taaatataac
      661 ggtttgcaca gcgtatgagg gctttttgct tggcaaaact cacaaaactc tcgggttgtc
      721 caaaataatt ggaacgtctc gtcttaactt cgacgatgag caaaaggtcg tccttgcggg
      781 caataatgtc gatttcttcg tgcccatatc gccaattacg agcttctatg gtataacctt
      841 gtttcaccag atattcggca gccagctctt cgccttcttt tcccatttcg ttatgttcgg
      901 ccatcattgt aagatttttt taatacaaat aaatcatttc agtaccttca tccgtgattt
      961 ctattctaat ttccgcaccc agcataagag gttcattgtg tggttgatgc ccagccggaa
     1021 atccaaaaca aaccggat
//
LOCUS       DAORPD010000787         1036 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_31714_length_1036_cov_3.081549, whole genome shotgun sequence.
ACCESSION   DAORPD010000787 DAORPD010000000
VERSION     DAORPD010000787.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31714_length_1036_cov_3.081549"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1024
                     /locus_tag="PK976_08560"
     CDS             <1..1024
                     /locus_tag="PK976_08560"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67981.1"
                     /translation="ENLSGNRAQAAKNVLNTDFDKMRKDKKNPVAVPTDLKITTQGNG
                     PDWDGFMKLVEASDLKEKGTILNIVNMADPKKKEQEIRNMILIYPQLEKDLLPPLRRA
                     EIVITAFEPSKTDAEILSSATTEPEKLTEKELLYAATLTQDLKTQQKIYRSATQLHPN
                     SWIAFNNAGVVEIKLGNINEAGADIEKANSLAPDQAEVINNLGALAMLKGDNAKAKQM
                     FEKAKKLGANENYNLGVLMIPEGKYSEAVASMKGEKCDYNLALALMLSGNNGEAVKAL
                     ECAEKNAQVYYLTAVLGARTGNITLMLDNLGMAIKADGKMKQEAKNDREFLKFANTPE
                     FKKLVE"
BASE COUNT          362 a          200 c          225 g          249 t
ORIGIN      
        1 cgaaaacctt tcgggcaatc gtgcccaggc ggctaaaaat gtactgaata ccgattttga
       61 taagatgaga aaagataaga aaaatcctgt tgccgtacct accgacttaa aaattactac
      121 gcaagggaat ggtccggatt gggatggatt catgaaacta gttgaagcca gcgacctgaa
      181 agaaaaggga acaatcctga atatcgttaa tatggccgat ccaaagaaaa aagaacagga
      241 aatccgcaat atgatcctga tttatcctca actcgaaaaa gatcttttgc ctcctttgag
      301 aagagccgaa atcgtaatta cagcattcga accttcgaag acagatgccg aaatcctgag
      361 ctcggctact actgaacccg aaaaacttac cgaaaaggaa cttctttatg ctgctacact
      421 cactcaagac cttaaaaccc agcaaaagat atatcgttcg gctacacaat tgcatcccaa
      481 tagctggata gccttcaaca atgctggggt agtcgaaatt aaactcggta acatcaatga
      541 agccggagca gacattgaaa aggccaatag cttagctcct gatcaggctg aagttatcaa
      601 caatttaggg gcattagcca tgctgaaggg cgataatgca aaagccaaac agatgttcga
      661 aaaagcgaaa aaattaggag ccaatgaaaa ctataacctt ggtgtgttga tgattcccga
      721 agggaaatac agtgaagccg tggcttcgat gaaaggtgaa aagtgcgatt acaatcttgc
      781 actcgccctt atgctttcgg gaaataatgg tgaagcagtg aaagctttag aatgtgccga
      841 aaagaatgct caggtttatt atcttactgc tgttttaggt gctcgcacag gtaatattac
      901 cttaatgctc gataatttgg gaatggctat taaggctgat ggtaaaatga aacaagaagc
      961 caagaatgat agagaattcc ttaagtttgc caatactccc gaattcaaaa aattagtcga
     1021 atagagatat tttagg
//
LOCUS       DAORPD010000788         1035 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_31760_length_1035_cov_2.339796, whole genome shotgun sequence.
ACCESSION   DAORPD010000788 DAORPD010000000
VERSION     DAORPD010000788.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31760_length_1035_cov_2.339796"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1007)
                     /locus_tag="PK976_08565"
     CDS             complement(<1..1007)
                     /locus_tag="PK976_08565"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HRC67982.1"
                     /translation="MKRFREVAQQTTLLVKKYRGSLSGEHGDGRLRGEFIPLMVGERN
                     YRHLKEIKNTWDPEGIFNDRKIIDTPPMDTSLRYEPGKATKDIETIFDFSNDRGIIRA
                     VERCNGSGDCRKTEVMGGTMCPSYMATRDENASTRARANLLREMLTHSEQKNPFNHKE
                     LYEIMDLCLSCKACKSECPSNVDMAKLKAEFLQHYYDANSIPFRTWAVANISTLYRLG
                     SLIPDFTNFLLANKFTSSLLKRILNFAPQRQIQTLSGVNIKKQITRYQNSKMFPKGKV
                     YFFNDEFTHYTEAHIVIKAIMLLNRLGYEVIIPQHKPSGRSFISKGLLRRAKKLAISN
                     I"
BASE COUNT          272 a          208 c          213 g          342 t
ORIGIN      
        1 aaaatgttac taatagctaa tttttttgct ctgcggagga gtcctttcga aataaaggaa
       61 cggccgctcg gtttatgctg tgggataatc acttcatagc ccagacgatt cagtaacatg
      121 atggctttta taacgatatg ggcttcggta taatgggtaa attcatcgtt aaaaaaatag
      181 accttaccct tgggaaacat ctttgaattc tgataacgag ttatttgctt cttgatattt
      241 acaccactga gtgtttgtat ttgtctttga ggggcaaaat tcaggattct cttcaaaaga
      301 gaggaggtaa atttattggc aagtaagaag ttagtgaaat caggaatcag gctacccaat
      361 cgataaagag tcgaaatatt ggctacagcc catgttctga agggaatgct gtttgcgtcg
      421 taataatgct gaagaaattc ggcttttaat tttgccatgt ctacattcga tgggcattct
      481 gatttgcatg ccttgcacga taagcataaa tccattatct catagagttc tttgtggttg
      541 aatggatttt tctgttcgga atgggtaagc atttcacgca gtaaatttgc tcgggcgcga
      601 gtgcttgcat tttcatcgcg agtagccata taacttgggc acatggttcc tcccatcact
      661 tctgtctttc ggcaatcgcc cgaaccgttg catctctcaa ctgccctgat aattcctcga
      721 tcgttcgaaa aatcgaatat ggtttcgata tctttggttg ccttccctgg ttcgtaacga
      781 agcgaagtat ccataggagg agtatcgatg atctttctat cattgaatat tccttcagga
      841 tcccatgtgt ttttaatttc ttttaaatgt ctgtaattac gttccccaac cattagagga
      901 atgaattcac cccgtaaccg tccgtcacca tgttctccac tcaatgaacc tcggtatttt
      961 tttactagca aagttgtttg ctgagccact tctcgaaagc gtttcacatc ttccgggtct
     1021 tttaggttca aaaca
//
LOCUS       DAORPD010000789         1035 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_31778_length_1035_cov_1.794898, whole genome shotgun sequence.
ACCESSION   DAORPD010000789 DAORPD010000000
VERSION     DAORPD010000789.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31778_length_1035_cov_1.794898"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..821
                     /locus_tag="PK976_08570"
     CDS             <1..821
                     /locus_tag="PK976_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67983.1"
                     /translation="FHMSGYNQQPAGAVIPPIECTGSNEIVFTRSSVTGSQFGLILFT
                     RSGNQGSFSVNPPTFTISSSNFQTVPGTAGEWVYARMGNMSGVNSNTAYRISNSSGLF
                     HLGVLYGNSSNNARYGFFSNFASLNLGPDRTICYGDSTYLDAGAGRESYLWSTGETTS
                     SIWVKDAGTYWVDVTEDLCILSDAIIISHYPSTPVNLGQDVEICEGQTATFDAGAGYV
                     NYLWVPGNVHDQYYTTGTAGTYSVTVEDENGCHYSDTIQLTVHPLPPPKPIKHN"
     gene            843..>1035
                     /locus_tag="PK976_08575"
     CDS             843..>1035
                     /locus_tag="PK976_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67984.1"
                     /translation="MITPMILTGCKKLFTTQILILFLLLSTNLLAEGTKQIMPTTSSF
                     GELQLMPSFSNFAWFDCGVD"
BASE COUNT          284 a          214 c          211 g          326 t
ORIGIN      
        1 tttttcacat gtcgggctac aatcagcagc cagccggagc agttattcca cctatagaat
       61 gcacgggttc taacgaaatt gtatttacaa gaagcagtgt tacaggttca cagtttggct
      121 tgatcttatt tactcgttca gggaatcagg gatctttttc tgtcaaccct ccaactttta
      181 ccatatcttc gagtaatttt caaacagttc cgggtactgc aggcgaatgg gtttatgcac
      241 gcatgggaaa tatgtcgggt gtcaattcca acacagctta tcgcatttcg aattcttcgg
      301 gattattcca tctcggcgta ttgtatggca attcctccaa taatgcccgt tatggctttt
      361 tcagcaattt tgcgtcttta aatttaggcc cagataggac tatttgctat ggtgatagca
      421 cttacttaga tgcaggagcg ggaagggaaa gctatctatg gagtacaggc gaaacaactt
      481 cttctatctg ggtgaaggat gcaggaacct attgggttga tgttacagaa gacctttgca
      541 ttctgtctga tgccataatc atttctcact acccatctac tccagttaat ttgggacaag
      601 atgtcgaaat ttgtgagggg caaactgcta cattcgatgc cggtgcgggc tatgtgaatt
      661 atctttgggt gccaggaaat gttcatgatc aatattatac tacgggaaca gcgggaactt
      721 attcggtgac cgttgaagat gaaaatggat gccattatag cgatacgatt caacttacgg
      781 ttcatcctct acctcctccc aaacctatta agcacaactg aattttttaa attaactctc
      841 tattgataac tcctatgata ctaactggct gcaaaaagct atttactaca caaattttaa
      901 tcctgtttct tctcttatct acaaatttat tagccgaagg taccaaacaa attatgccta
      961 caaccagcag ctttggagaa ctacaactta tgccatcatt tagcaatttt gcatggttcg
     1021 attgtggagt tgatg
//
LOCUS       DAORPD010000790         1033 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_31896_length_1033_cov_2.096115, whole genome shotgun sequence.
ACCESSION   DAORPD010000790 DAORPD010000000
VERSION     DAORPD010000790.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31896_length_1033_cov_2.096115"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..946
                     /locus_tag="PK976_08580"
     CDS             140..946
                     /locus_tag="PK976_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67985.1"
                     /translation="MHIYRFRITSDSIEDFVREIDISPNQTFEDFQRVFLQAAPLSAE
                     EKTYFYKANARWVKQYEIAEQPLLSISKEKEGDDVRLEEHRLKIPYTHISLARFRDFV
                     DDPHQKFIIEYRGPDIYLFFMEMVKILETDSLANYPKCVTQKGEIPVKPIIIPEEIQE
                     KTRKQREERLTITSLNDDIPEEMIEEQMKELMEDEVFGAIMSGKEVPPSSNISRKMTA
                     REIAEEFGPEFADMMSTDADEKFDDEDITDEDIDDEFADDKSGEDFDDDF"
BASE COUNT          342 a          177 c          201 g          313 t
ORIGIN      
        1 ctaagatata aactccagac caagagatta ataattttat attttgattt tattgaggtt
       61 tgattcattc ccttaacttt gcacgctatt ttctataaat gtttttattg atttttacct
      121 tcttttaaat caatcagtta tgcatattta tcgttttcgg attacttctg attccatcga
      181 agattttgta agagagatcg atatttcgcc caatcagact ttcgaagatt ttcaaagggt
      241 ttttctccaa gctgctcctt tatcggcaga agagaaaaca tatttctata aagccaatgc
      301 acgttgggta aaacaatatg agatagcaga acaacccctg ttatccattt cgaaagaaaa
      361 agaaggcgat gatgttagac tcgaagagca tcgtttgaaa attccctata cgcatatcag
      421 tcttgcacgt tttagggatt ttgtggatga ccctcatcaa aagtttatca tcgaatatcg
      481 aggtcctgat atttatttgt tttttatgga aatggtaaaa atactggaaa ccgattcttt
      541 agcaaactat ccaaagtgtg taacacaaaa aggagaaata cccgtaaaac ccatcatcat
      601 acctgaagaa attcaggaaa agacacgtaa acaacgagaa gaaaggctta ccattacttc
      661 tttgaatgat gatattccgg aagaaatgat agaggaacag atgaaagaat taatggaaga
      721 tgaggttttt ggtgcgataa tgagtgggaa ggaagtccct ccatcttcca atatttcacg
      781 caagatgaca gccagagaaa ttgccgaaga atttggaccc gagtttgccg atatgatgtc
      841 gactgatgca gatgaaaaat tcgatgacga agatattacc gatgaagaca tagatgacga
      901 atttgccgac gataaatcgg gcgaagactt cgatgacgat ttctaatgtg tcttcaattt
      961 tctctacatc caaatcatga aacttcagaa acctttattg gttgtgattg cagggccgac
     1021 tgcttcgggg aaa
//
LOCUS       DAORPD010000791         1031 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_32004_length_1031_cov_2.097336, whole genome shotgun sequence.
ACCESSION   DAORPD010000791 DAORPD010000000
VERSION     DAORPD010000791.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32004_length_1031_cov_2.097336"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(277..>1031)
                     /locus_tag="PK976_08585"
     CDS             complement(277..>1031)
                     /locus_tag="PK976_08585"
                     /inference="COORDINATES: protein motif:HMM:NF017396.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HRC67986.1"
                     /translation="LNFSSEDERVYKFLDSVGTPECRERIHDFQVQLFERKNEILPLF
                     EELAASKGWNFTMGLERAYILSILEYSFSFWQWGFSCSSIPASSSTAKELFKHWLDVN
                     PFTFFEENEVDKVRPFFYQAMTEIGMYGYRVKPFSRYLSDTTNITFSFTMPKGHQAVF
                     NASTMRDIDRWIKDSGNYMLYIYGQNDAWSATAVDPGGKTNAVKMVCPGGSHSSRIKD
                     FPPELQDSIYRVLGRWLDLRLPANGDIKLNPD"
BASE COUNT          311 a          226 c          216 g          278 t
ORIGIN      
        1 aagccaaacc aaatcgactt aggcaatccc atcgcaaatt gcagttatat ggttaaccca
       61 agacatcaag gacttgggat ttgttggcac ttacataatg tgtaaagatt tgacaaggaa
      121 tgaataaacc tatcgctaac ggtagaaaaa atagataatt aaagttgcat atctgctcaa
      181 gaatacgata atttgaagta tgaggaaact tttgtaaaaa atcgatttgc aataaaagga
      241 tcaggggttc tgaaatttca tcatgataaa tgattactaa tcagggttta gtttaatgtc
      301 gccattagcc ggcaatctaa ggtcgagcca tctgcccagg actctgtaaa tgctatcctg
      361 aagttcggga ggaaaatcct taatccttga cgagtgcgaa ccgccgggac aaaccatttt
      421 caccgcatta gtctttcccc ctggatcaac agccgtagcc gaccatgcat cgttttgtcc
      481 gtatatgtag agcatgtaat tacccgaatc cttaatccaa cgatcaatgt cgcgcattgt
      541 ggaggcgttg aaaaccgctt gatgcccctt tggcatagta aaactaaagg ttatattggt
      601 ggtatcgctg aggtagcgac tgaacggttt caccctgtag ccgtacattc caatctcggt
      661 catagcttgg taaaagaaag ggcgaacctt gtccacttca ttctcttcga aaaaggtaaa
      721 ggggttaaca tcgagccaat gcttaaaaag ctcctttgcg gttgaactac ttgcgggaat
      781 ggacgagcag ctaaatcccc actgccagaa tgaaaaagag tactccagaa tgctcaggat
      841 ataggccctt tcgagtccca tcgtgaaatt ccaccccttg cttgctgcaa gctcctcgaa
      901 aagtggaaga atttcatttt tcctttcgaa aagttgcacc tgaaaatcgt gtatcctttc
      961 acggcattca ggcgtcccaa cactatcaag aaatttgtac accctttcgt cttcgcttga
     1021 aaagttcagt g
//
LOCUS       DAORPD010000792         1030 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_32064_length_1030_cov_2.104615, whole genome shotgun sequence.
ACCESSION   DAORPD010000792 DAORPD010000000
VERSION     DAORPD010000792.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32064_length_1030_cov_2.104615"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="PK976_08590"
     CDS             complement(<1..420)
                     /locus_tag="PK976_08590"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HRC67987.1"
                     /translation="MLVSTREYKASLKALEIEEYFDLWIYRPLGYIVAKLVQSISVTP
                     NQLTVLAMFCGIASGYVFSLGTQQAFILGALLYFMFNILDCSDGQLARIKGTSSVAGK
                     ILDGAADYIAGIAIYIGIGIGYVEYVSNPVGWWILLAL"
     gene            complement(618..>1030)
                     /locus_tag="PK976_08595"
     CDS             complement(618..>1030)
                     /locus_tag="PK976_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HRC67988.1"
                     /translation="IDYYIKMYFSFIVLDFISVVVDEHDKVAAFGITMPSLSKAVQKS
                     GGKILPFGFIHILRAMKKNDTVDFYLTAVRPELQGKGVNALLFIDLIPKYRKFGIKKA
                     ESNVELENNQMVRRQWDMFPKRQHKRRRAYIKHI"
BASE COUNT          292 a          211 c          197 g          330 t
ORIGIN      
        1 cagtgctagc agtatccacc atccaactgg attagaaaca tattcgacat agcctattcc
       61 tataccaata tagattgcga tgccggcgat ataatcggcg gcaccatcca gaattttacc
      121 tgcaaccgat gatgttcctt ttattcttgc caattgtcca tcgctgcaat ctaatatatt
      181 gaacataaaa tagagcaagg cacccagaat aaatgcctgc tgggttccca gggagaagac
      241 atatccgctg gcgataccgc aaaacatggc taaaaccgta agctggttgg gtgttacact
      301 tattgactga accaattttg ccactatata tcctaaaggc cgataaatcc ataaatcgaa
      361 atactcctca atttctaaag ccttgagtga agccttatat tctcgggtac ttacaagcat
      421 tgtttatggt tcgaattgat gtggtctaca aaaatataaa atttcacaaa atctcattat
      481 tcgtcagctc ttggggaaac atcatctcgt cgatatttca ttgtaaactg atgattatta
      541 tttgaaaagg aattgatttt ttgtttggca aatctgttca ctgaagtttt gtttcgattc
      601 attggtcgta caggtgatca gatgtgcttt atataggctc gtcttcgctt gtgttgtctt
      661 ttggggaaca tatcccactg ccgccttacc atttggttat tctcgagctc aacattgctt
      721 tcggcttttt ttatcccaaa ttttctgtat ttgggaataa ggtcaataaa caatagtgca
      781 tttactcctt ttccctgaag ttctggtctt acggctgtca agtaaaaatc gaccgtgtcg
      841 tttttcttca tggctcgtaa aatatggatg aagccaaaag gcagaatctt gccacctgac
      901 ttttgtacgg ccttgctcag tgatggcatg gtaatgccaa aagctgcaac tttgtcgtgt
      961 tcgtcaacga caaccgaaat aaaatcgagt acgatgaacg agaaatacat tttgatgtaa
     1021 tagtcgattt
//
LOCUS       DAORPD010000793         1030 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_32079_length_1030_cov_1.664615, whole genome shotgun sequence.
ACCESSION   DAORPD010000793 DAORPD010000000
VERSION     DAORPD010000793.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32079_length_1030_cov_1.664615"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="PK976_08600"
     CDS             <1..552
                     /locus_tag="PK976_08600"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HRC67989.1"
                     /translation="GWFEKYKVTMQSMGALALGLVYISLTLGLFLHLRLSEDIRVLND
                     GEEWLGRSNGFYIPILIILALWVNDTMAYLVGSAIGKTPFSKISPKKTIEGTVGGALL
                     CILALTLILKPWFRWPVLLGISVIVAIFGTMGDLLESKLKRMANVKDSGKIMPGHGGF
                     LDRFDSLLGSIIFIWLFLNLVKF"
     gene            complement(549..>1030)
                     /locus_tag="PK976_08605"
     CDS             complement(549..>1030)
                     /locus_tag="PK976_08605"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRC67990.1"
                     /translation="PMNVYGTQPTLDRVITEYDYAFHDFRYPGVPSIQLHTLNEEDDF
                     TIGDIKISPIPVWHLNMLVLGFRFGDFTYITDANKIDEPSKEKIKGSSVLVLNALRKE
                     KHISHFTLQEAIDLADELEIPQVYFTHVSHQMGLHEDTNRQLPRSRQLAFDGLRIEI"
BASE COUNT          275 a          197 c          236 g          322 t
ORIGIN      
        1 ggttggtttg aaaagtataa agtaacgatg caatcaatgg gagcactggc tttaggattg
       61 gtatatattt cgctcacatt gggccttttt ctgcatctgc gtctgtcaga agacatcaga
      121 gtactgaatg atggggaaga atggttaggc aggtcaaatg gtttttatat ccccatcctc
      181 attattcttg cgctatgggt aaatgataca atggcttact tagtaggttc tgccatcggc
      241 aaaacgcctt tttcaaaaat ttcacccaaa aagactatcg aaggaactgt gggaggggca
      301 ttactttgca tactggcatt gacccttatt ttaaagccct ggtttcgctg gcctgtgcta
      361 ttaggcatct cggtgatagt ggcaatcttt ggtactatgg gtgatctgct tgaatctaaa
      421 ctaaaaagga tggcaaatgt gaaagacagt ggaaaaatca tgcccgggca cggtggtttt
      481 ttagaccggt tcgactcact tttgggtagt attattttta tctggctgtt tctgaactta
      541 gtgaaattct aaatttcaat ccgcaatcca tcaaaagcaa gttgtcggct ccggggcagt
      601 tgtcggtttg tatcttcgtg caaacccatc tgatgcgaaa catgggtaaa atacacctgc
      661 ggaatctcca actcatctgc cagatcaatg gcttcttgta gcgtaaagtg ggaaatatgt
      721 ttttcttttc gaagtgcatt taaaaccagc acactgcttc cttttatttt ttcttttgac
      781 ggctcgtcta ttttattggc atcggtaatg taggtaaaat cgccaaaacg gaagccgagt
      841 accagcatat tgagatgcca gaccggaatt ggagaaattt taatgtcgcc gattgtaaaa
      901 tcatcttctt cgttaagagt atgcaattga atacttggca cgccgggata cctgaaatca
      961 tgaaaggcgt aatcgtactc ggtaataact ctgtcgagcg ttggttgtgt tccatacaca
     1021 ttcattggct
//
LOCUS       DAORPD010000794         1029 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_32112_length_1029_cov_2.303901, whole genome shotgun sequence.
ACCESSION   DAORPD010000794 DAORPD010000000
VERSION     DAORPD010000794.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32112_length_1029_cov_2.303901"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1029
                     /gene="sppA"
                     /locus_tag="PK976_08610"
     CDS             <1..>1029
                     /gene="sppA"
                     /locus_tag="PK976_08610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HRC67991.1"
                     /translation="LASLADQIWLNPEGSIELKGLMVDAVFLKKMLEKLDIEAQIIRH
                     GKYKSAVEPFMLDKMSPENREQTFQYVSTIWKNIASEIAEGRNLSVEKLDKITDSLLA
                     INPQKALELGIVDRLVYKDEIEDLLLSQQNIDTTSKINYLDFKDYFSAPEPVKKTIDR
                     SKRIAVIYALGDVIDAKGDEQSIGTKNIPEAIQKAREDSKVKAVVLRVNSPGGSALTA
                     DLIWREIELTKVIKPVVASFGDVAASGGYYIACNATKIIASPNTITGSIGVFGVIPNA
                     QKFFNNRLGLTFDAVKTNENSDFAGINRPLTAYQKQVILDEIEEIYSTFVSHVSDGRG
                     MNEVTVDSL"
BASE COUNT          326 a          204 c          222 g          277 t
ORIGIN      
        1 ttggcctcgc tggctgatca aatctggttg aatcctgaag gaagcataga gttaaaagga
       61 ttaatggtgg acgcagtgtt tctgaaaaaa atgctggaaa agcttgatat cgaagcacaa
      121 atcatacgtc acggaaaata caagagtgcc gtagaaccgt ttatgctcga taaaatgagc
      181 cctgagaaca gagaacaaac ctttcagtat gtaagtacca tctggaaaaa tatcgcctcc
      241 gaaattgccg agggtcgaaa tctttctgta gagaagctcg ataaaattac cgacagtctt
      301 ttggctataa acccgcaaaa agccttagaa cttggcattg ttgaccgttt ggtctataaa
      361 gatgaaatcg aagatctttt attgtcgcaa cagaacatag atactacttc caaaatcaat
      421 tatttagact ttaaagatta tttcagtgct cctgaacccg taaagaaaac catagatcgt
      481 tccaaaagaa ttgctgtaat ttatgcctta ggcgatgtaa ttgatgcaaa aggtgatgag
      541 cagagtattg gtactaaaaa catacccgaa gccattcaga aggcacgaga agatagtaag
      601 gtaaaggcag tggtgctacg agtgaacagt cccggtggaa gtgccctcac ggccgatctc
      661 atctggcgcg aaattgaact cacgaaagtc atcaaacctg ttgtggcttc atttggcgat
      721 gtagctgcct caggaggata ttatattgcc tgcaatgcca ctaagatcat tgcttcaccc
      781 aatactatta caggctcaat tggagttttt ggcgtgattc ccaatgcaca gaaattcttc
      841 aataatcgct tgggcctcac tttcgatgca gtaaaaacca atgaaaattc cgatttcgcc
      901 ggtattaacc gccccttgac tgcttatcaa aaacaagtta ttcttgatga aattgaagag
      961 atttactcga cttttgtgtc tcatgtttcc gatggccgtg gaatgaatga agttacagtg
     1021 gatagcctc
//
LOCUS       DAORPD010000795         1029 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_32114_length_1029_cov_2.275154, whole genome shotgun sequence.
ACCESSION   DAORPD010000795 DAORPD010000000
VERSION     DAORPD010000795.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32114_length_1029_cov_2.275154"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="PK976_08615"
     CDS             complement(<1..480)
                     /locus_tag="PK976_08615"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRC67992.1"
                     /translation="MNNKNIYVLSLALIVVVVIWGCSNKQEHLKNEITNIEKQLFNDT
                     TGIIMPQKAQKTIELYSKYIHNYPHDSLSAEYIFRAAQLCTAIGNFEEAIKFYDSLII
                     SFPEIPKAAEAMFMKAFVFDNYLYRYDKARENYMLFIQKFPNHDLRRDAEKSIQFLGM
                     "
     gene            complement(499..>1029)
                     /gene="def"
                     /locus_tag="PK976_08620"
     CDS             complement(499..>1029)
                     /gene="def"
                     /locus_tag="PK976_08620"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HRC67993.1"
                     /translation="QKAADIKSDFPDLQEFIKNLWETMYATDGIGLAAPQVNRAIRIF
                     VIDATVLAKNYPETTGFKKTFINAYKINEGGDAWLFNEGCLSIPDIHEDVSRPKRILL
                     HYLDENFDEHEEWFEGAVARTIQHEYDHLEGILFTDYLSPMRKTMLARKLNNIALGNI
                     ETKYRMIFPRSKKQKR"
BASE COUNT          273 a          186 c          215 g          355 t
ORIGIN      
        1 catccccaaa aactgtatcg atttttcggc atctcttctc aggtcgtgat tggggaattt
       61 ttggatgaat aacatataat tttcacgggc tttgtcataa cgataaagat agttgtcgaa
      121 gacgaaggct ttcataaaca tggcttcggc agctttagga atctcgggaa atgaaatgat
      181 caggctgtcg taaaacttga tagcctcttc gaagttgccg atggcggtac acaactgagc
      241 cgctctgaaa atatactcgg cagaaaggct gtcgtgtgga taattgtgta tatatttaga
      301 gtataactcg atggtttttt gtgctttttg tggcattatt atgccagtag tgtcattaaa
      361 gagctgtttt tcgatgttgg tgatttcgtt ttttaaatgc tcctgtttgt ttgaacagcc
      421 ccatataact acaacaacga tcagggctaa tgacaatacg taaatatttt tattattcat
      481 ggctttgtga attaatggtt atctcttctg tttttttgat ctcgggaaaa tcatcctgta
      541 ttttgtctct atgttaccta atgcaatatt gttgagcttt cttgcaagca tggtttttcg
      601 cataggactt aggtaatcgg taaagagaat tccttctaaa tgatcatatt catgctgaat
      661 ggtacgtgct acagcacctt cgaaccattc ttcgtgttcg tcgaagtttt cgtcgaggta
      721 gtgcaaaagg atgcgttttg gacggcttac atcttcatgt atgtcaggaa tgctcaagca
      781 accctcgttg aatagccagg cgtctcctcc ttcatttatt ttataggcat ttatgaaagt
      841 tttcttaaat ccagtggttt cgggatagtt tttagccaga acagtggcat cgatcacaaa
      901 tatgcgaatt gcacgattca cttgaggtgc agccaaacca attccatcag tggcatacat
      961 ggtttcccac aaatttttaa taaattcttg caaatcggga aaatcggatt taatatctgc
     1021 agctttttg
//
LOCUS       DAORPD010000796         1028 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_32195_length_1028_cov_1.550874, whole genome shotgun sequence.
ACCESSION   DAORPD010000796 DAORPD010000000
VERSION     DAORPD010000796.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32195_length_1028_cov_1.550874"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..>1028
                     /locus_tag="PK976_08625"
     CDS             62..>1028
                     /locus_tag="PK976_08625"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HRC67994.1"
                     /translation="MSFLVINVVLSAFISATTAKAAILLPIFMVIAAIYGARGGDQRN
                     NFGRSIVLQNLLYINVGASGFLTGSGANLLAVSIIAGATGARIFFSDWLMAMFPMAIG
                     LMLIGYLLAMRVFFPLKPEERLPQIEGGMDRLREVYNELGPMSRKEKKAIILFITILA
                     FWSTDRLHGISPTAVAFLGAIVALLPRIGILTWNEVDIPWHLMLFSAGAYTLGAGFSA
                     TDLPAISVNAFFDHLGMTQNTPFWALYLLLTGVMVYSALLFESKTMRAMIFVPIAIGV
                     ATRFGYSIESLALPVAFMTEHVYVLPFNSKPAALLYETGHYSLTDT"
BASE COUNT          231 a          228 c          239 g          330 t
ORIGIN      
        1 tgccaagagg tttgctctgt ggtttctact gaaatttgga caaagtgcat cgggaatttt
       61 tctgagtttt ttggtaatca acgtggtttt atcggccttc atatcagcca ctacggccaa
      121 ggctgccatt ttattgccta ttttcatggt tatagctgca atttacggag ctcgaggagg
      181 agatcagcgc aataacttcg gccgaagtat agtgttgcaa aacctcttat acattaatgt
      241 aggggctagt ggatttctta cgggctcggg agccaatcta ctggcagttt ctattattgc
      301 gggtgcaacg ggcgcaagga tatttttctc cgattggctt atggcgatgt ttcccatggc
      361 cattggtttg atgctcatag gatatttatt ggctatgaga gtatttttcc ccttgaaacc
      421 cgaagaacgt ctccctcaaa tcgaaggtgg gatggatcgt ttgcgagagg tttataatga
      481 acttggccct atgagccgaa aagaaaaaaa agctatcatt ctctttataa ccattctggc
      541 tttttggtca accgatcggc tgcatgggat tagccctacg gcagttgcat ttttaggtgc
      601 catcgttgct ctattgcccc gtattggtat ccttacatgg aatgaggtcg atattccctg
      661 gcatctgatg cttttctcgg ccggagctta tactcttgga gccggatttt ctgctaccga
      721 tcttcctgcc attagcgtaa atgctttttt cgatcatctg ggcatgacac aaaatactcc
      781 tttctgggct ctttatcttt tgctgacggg cgttatggta tacagcgccc tcttattcga
      841 atcaaaaacc atgcgggcta tgattttcgt tcctatcgct atcggagtag ctacccgctt
      901 cggatacagc atcgaaagtc ttgcattgcc agtggccttt atgactgaac atgtttatgt
      961 actcccgttt aactcgaaac cggcggcact gctctatgaa accgggcatt acagcttgac
     1021 cgatactt
//
LOCUS       DAORPD010000797         1026 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_32295_length_1026_cov_2.173018, whole genome shotgun sequence.
ACCESSION   DAORPD010000797 DAORPD010000000
VERSION     DAORPD010000797.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32295_length_1026_cov_2.173018"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1026)
                     /locus_tag="PK976_08630"
     CDS             complement(<1..>1026)
                     /locus_tag="PK976_08630"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HRC67995.1"
                     /translation="QRKARYGEALELIQTSLHNQQPYVLPITYVGEAVLQGPEIILWA
                     MRSSNLPQLMEKIEKAKGTEKEELNKKFQNTLEKLSKRAEDFYKDYNPSTDKKLFAQM
                     LQLYYRDISPEMRADIFKDLEKKYKGDFDKWSDDIYKKSIFADEKRFMAFLQNPSLKV
                     LKKDPALEIGTEVRNHFILLYSQVENAQKEEHKGSRLYIAGLREMNPGKLFAPDANST
                     LRMTYGSILDYYPADAVHYDLQTTLTGVMEKEDPDNPEFIVDKKLKDLYEGKDFGRYG
                     TQIHGRTDIVTCFLSNTDITGGNSGSPVMNANGELIGLAFDGNWEAMSGDIAFEPNLQ
                     RTISVDIR"
BASE COUNT          278 a          215 c          188 g          345 t
ORIGIN      
        1 ccgaatatca accgaaatag tgcgctgtaa attaggctcg aaggcaatat ctccactcat
       61 ggcttcccaa ttaccatcga aagccaagcc aatgagctcg ccgtttgcat tcattaccgg
      121 gcttcctgaa ttgccccccg ttatatcggt attggaaaga aaacaggtta caatatcggt
      181 acgcccatga atctgagtgc catatctccc aaaatccttt ccctcgtata aatcctttaa
      241 ttttttgtcg actataaatt ctggattatc ggggtcttct ttttccatta ctccagttaa
      301 agttgtttgc aaatcgtaat gaacagcatc ggcaggataa taatcaagaa tactgccata
      361 ggtcattcgc aaagtactat tggcatcggg tgcaaaaagt ttcccgggat tcatttccct
      421 aagacctgca atataaagtc tgcttccttt atgttcttcc ttttgtgcat tttccacttg
      481 gctgtaaagc aaaataaaat gattcctcac ctctgtgcca atttccagtg caggatcttt
      541 tttcagcact tttaaagaag gattttgcag aaatgccata aaccgcttct catcggcaaa
      601 aatgcttttt ttataaatgt cgtcagacca tttatcgaag tcacccttgt attttttctc
      661 taagtcttta aaaatatccg cccgcatctc gggtgaaata tcacgataat acagttgcaa
      721 catttgagca aaaagttttt tatcggttga aggattgtag tctttataaa aatcttcagc
      781 tcttttagaa agtttttcga gagtgttttg aaattttttg ttgagttctt ctttttcagt
      841 tcccttagct ttctctattt tttccattaa ctgaggcaga ttggaacttc gcatagccca
      901 aaggataatt tcaggtcctt gtaaaacagc ttctcccacg taagtgatag gtaggacata
      961 gggctgttga ttatgaagag atgtttggat tagttcgagg gcttctccat agcgggcttt
     1021 acgttg
//
LOCUS       DAORPD010000798         1023 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_32468_length_1023_cov_2.150826, whole genome shotgun sequence.
ACCESSION   DAORPD010000798 DAORPD010000000
VERSION     DAORPD010000798.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32468_length_1023_cov_2.150826"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="PK976_08635"
     CDS             <1..482
                     /locus_tag="PK976_08635"
                     /inference="COORDINATES: protein motif:HMM:NF021439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2148 domain-containing protein"
                     /protein_id="HRC67996.1"
                     /translation="RLMMAAARTAPKGRGRDSLFIALASDDEMQQIAKTMKIFAEEWE
                     TQFFARDARNLLHSDALVLIGTKIEPLDLRKCGFCGFENCSEKNLHPNTPCAYNNIDL
                     GIAVGSAVAVAAQHHADNRVMYSVGKTALYMGLAKPDVKILLGIPLSIRSKNIYFDR"
     gene            complement(479..715)
                     /locus_tag="PK976_08640"
     CDS             complement(479..715)
                     /locus_tag="PK976_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67997.1"
                     /translation="MLSNGVSCFFNPAIMLHSSEAKKKAIELAKIVVSCCAKKVSSRL
                     DDNTAEAILAGIFVLKLREKNLVLPSEVKKLMRF"
     gene            complement(684..959)
                     /locus_tag="PK976_08645"
     CDS             complement(684..959)
                     /locus_tag="PK976_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC67998.1"
                     /translation="MGTLISIDTGVKLGMAVFNFEGKLLFYGSHNYGDINRLKRNIPR
                     ILKRYETVKLLVLEGGGELAEIWLLEAVRQQAEVLMIHAQQWRKLFF"
BASE COUNT          305 a          216 c          198 g          304 t
ORIGIN      
        1 ctcgtttaat gatggctgca gcgcgcacag ctcctaaagg cagaggtcgc gattcattat
       61 tcattgcttt agccagtgat gacgaaatgc agcaaattgc aaaaaccatg aaaatatttg
      121 ccgaagaatg ggaaacacaa ttttttgcac gtgatgcccg aaatcttttg cattccgacg
      181 cgcttgtact gatagggaca aaaatcgaac ctctcgattt gagaaaatgt ggattttgtg
      241 gtttcgaaaa ttgcagtgaa aaaaatctac accccaatac tccttgtgcc tataacaata
      301 tcgatctggg aattgcagtg ggttcggctg tagctgtggc agctcagcat cacgccgaca
      361 acagggtgat gtattcggtt gggaaaacgg ctttgtacat gggtctggcg aaacctgatg
      421 taaaaatact tctgggaatc cctctcagta ttcgttctaa aaatatctac ttcgatcgtt
      481 agaaacgcat caacttttta acttctgaag gaagaaccaa atttttctct cttaatttca
      541 gcacgaaaat tccagccaaa atggcttcgg cagtattatc gtcaagacgt gatgaaacct
      601 ttttggcaca gcatgaaacc acaattttgg ccaattctat ggcctttttc ttggcttcgc
      661 tgctatgcag cataatggcg ggattaaaaa aacaacttac gccattgctg agcatgaatc
      721 ataagaactt cggcttgttg ccttacagct tccaacagcc agatctcggc taattctccc
      781 cctccttcca gaacaagcag ttttaccgtt tcatatcttt tcaaaattcg aggtatattt
      841 ctttttaaac gatttatatc accataatta tgtgatccat agaaaagtag cttgccttca
      901 aagttaaaaa ctgccattcc aagttttaca ccagtatcga tggagatcag ggtacccatg
      961 tctaagttta aaaatttagg taaaaatctt cggtaagtct cctatattct tgagtataag
     1021 aat
//
LOCUS       DAORPD010000799         1022 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_32521_length_1022_cov_2.341262, whole genome shotgun sequence.
ACCESSION   DAORPD010000799 DAORPD010000000
VERSION     DAORPD010000799.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32521_length_1022_cov_2.341262"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1022
                     /locus_tag="PK976_08650"
     CDS             <1..>1022
                     /locus_tag="PK976_08650"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HRC67999.1"
                     /translation="IGKVKWYENITMSYSMNAENRINTYDSLLFKHETLKNMQNGIKH
                     SIPISSSIKVLKYFNLTNSINYTERWYFRTIHKAWVDTTDWSHDTVASGYVRTDTAYG
                     FKCARDFTFSSSLNTRIYGMFTFMKGPIVAVRHVVSPSVSFSYNPDFSSSFWGYYKPY
                     QKNAKGTIDKYSIFEGGIYGSPIGSKAGRISFSISNNLEMKVRNRKDTLTGVKKIPLI
                     DNLTISTSYDFMRDSLRWSKVQMSGRSTLFKNITLNYSSSWDPYILDSTGNKNLNQYE
                     WKVNRRPLRLDNTNWNLSLNWNFSSKSKGSGGKPPLNPNDPEYQDYLLNPDSYIDFDI
                     PWKLNLA"
BASE COUNT          328 a          195 c          202 g          297 t
ORIGIN      
        1 attgggaaag taaagtggta tgaaaatatt accatgtcgt attctatgaa tgcggaaaac
       61 cgtatcaata cttacgattc gttgcttttt aagcacgaga cattgaaaaa tatgcaaaat
      121 ggcattaaac atagtatacc cataagcagt tctataaagg ttctcaaata tttcaacttg
      181 actaattcaa tcaattatac cgaaagatgg tatttcagaa ccattcacaa agcctgggtc
      241 gatactaccg actggagcca cgacacggtt gcttcaggat atgttcgtac cgatacggcc
      301 tatggattca agtgtgctcg tgatttcact tttagcagca gtttgaatac ccgtatttat
      361 ggtatgttta ctttcatgaa gggacctatc gttgcagtgc gtcatgtggt gagccccagt
      421 gtgagttttt cgtacaatcc cgattttagc agttcattct ggggttatta caaaccttac
      481 cagaaaaatg cgaaaggtac tatcgataaa tattccatat ttgagggcgg aatatacgga
      541 agccctatag gatcaaaggc aggccgaatt tcattttcaa ttagcaataa cctcgaaatg
      601 aaagttagaa accgtaaaga tacccttacg ggggtaaaaa agataccctt gatcgataat
      661 cttaccatca gtacttctta tgattttatg cgagatagcc tgcggtggtc gaaagtgcag
      721 atgagcggcc gcagtacact ttttaaaaac attaccttga attattccag ttcgtgggac
      781 ccttatattc tggatagtac cggaaacaaa aatcttaacc aatacgaatg gaaagttaat
      841 cgaagaccct tacgactgga taatacaaac tggaatttat cattaaactg gaatttttct
      901 tccaaatcga agggtagtgg aggaaaacct ccgttaaatc ccaatgaccc cgaatatcag
      961 gattatttgc tcaatccaga tagttatatc gatttcgaca ttccatggaa actcaatttg
     1021 gc
//
LOCUS       DAORPD010000800         1021 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_32595_length_1021_cov_2.027950, whole genome shotgun sequence.
ACCESSION   DAORPD010000800 DAORPD010000000
VERSION     DAORPD010000800.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32595_length_1021_cov_2.027950"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /locus_tag="PK976_08655"
     CDS             <1..889
                     /locus_tag="PK976_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium/glucose cotransporter"
                     /protein_id="HRC68000.1"
                     /translation="GFNQYIIQRTLAAKSLKEAQHGIIFAAFLKILIPLIVVIPGIIA
                     FVLNADPTGHITPQSADPTFFNAMGAVDNDRAYPWLVKTFIPTGLKGLVVAALVAAIV
                     SSLASMLNSTSTIFTLDIYKPYFSKNASEKELVTVGRITSAIALIIAIFIAPLLGNIK
                     QAFQYIQEYTGIVSPGILAIFLLGLFYKKATNRSAIFGALFSIVFAFYLKIGQNGWMA
                     GTPLAGFFPSFPWMDQMLIVCLGTMLVMVIYSYFEARGVNDPKGIPLKKSTFHTDSVF
                     NILATVVILILTAIYAIFW"
BASE COUNT          270 a          208 c          201 g          342 t
ORIGIN      
        1 gggattcaat cagtatatca ttcaaagaac cttagcggcc aaatcgctaa aagaagcaca
       61 gcacggaata attttcgccg cttttctcaa aattcttatt cctttgattg tagttattcc
      121 gggaattatt gcctttgtac tgaatgctga tcccactgga catattaccc cccaaagtgc
      181 cgatcctacc ttttttaatg caatgggagc tgttgataat gaccgggcgt atccctggtt
      241 ggttaagaca tttattccaa caggactgaa gggtcttgtg gttgctgcgt tggtggccgc
      301 catagtttct tccctcgcct ctatgctgaa ttctaccagt acaattttta cactcgatat
      361 ctataaaccc tatttctcga agaatgcttc cgaaaaggaa ttggtgactg taggtcgaat
      421 tacttctgcg attgctctta tcattgcaat atttatagca cccttactgg gaaatattaa
      481 gcaagcattt caatatatac aggaatatac cggtattgtg agtccgggaa ttctggccat
      541 tttcctttta ggattattct ataagaaggc taccaatcgt tctgcaatct ttggagctct
      601 cttcagtatc gtgtttgctt tctatttaaa aataggacag aacggatgga tggcgggtac
      661 accacttgca ggatttttcc catccttccc atggatggat caaatgctga tcgtatgttt
      721 gggcaccatg ctggtgatgg ttatttatag ttattttgag gcacgaggtg tgaatgatcc
      781 aaagggaatt cctttgaaaa agagcacttt ccataccgat tcggtattca acattttagc
      841 tactgtggta attcttattt tgacggccat ttatgccata ttctggtaag gtttgtaaaa
      901 agaatgtttt aatatggcga aggatcgaac ttccttcgcc atattaaaat ctccctcaat
      961 ttatgaataa cctcttacct ttgccatccg tttaatcatt catgcgagtt gaatcatcaa
     1021 t
//
LOCUS       DAORPD010000801         1019 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_32705_length_1019_cov_2.379668, whole genome shotgun sequence.
ACCESSION   DAORPD010000801 DAORPD010000000
VERSION     DAORPD010000801.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32705_length_1019_cov_2.379668"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            391..>1019
                     /locus_tag="PK976_08660"
     CDS             391..>1019
                     /locus_tag="PK976_08660"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HRC68001.1"
                     /translation="MIKRIPLYVKILAGLAIGIFVGIIAVITNSGGFIIDYVKPFGTI
                     FLNLLKLIAIPLIFTSLVSGIAGLSNIKKFSTLGVQTFVLYIVTTVIAVCIGLGLVNL
                     IRPGDYFSEGKRSEMMAKYGQSISIDEKNVQNLGHRSPLQFLVDMVPENIVDAASSNT
                     SMLQIIVFAVLFGLALISINEQFSEPVKKFIVGLNEVILKIVDFIMLIAP"
BASE COUNT          288 a          172 c          191 g          368 t
ORIGIN      
        1 tgacactgga cctattccag ccggttcgac tctggtcttc gatgtagaag tagtggatat
       61 aaaataggga taaccctgtt catattgaat cgctttgcag cttgtcattc gattcgatgt
      121 tttcttgtaa aatattcaat aataatttcg gtgctcgaaa gaagttaacc gtgctcaata
      181 tttgtacaac tttcaatttc atatttatct ttcaccgatc ttcatcctct cgtttctatg
      241 taaaacaaat ccacttttgt tatagtttgg ccaagttcct acttttgcgg atgaatagga
      301 cagaaactga ttcgaacctt aattttttgg gttgaagcat tgaaaattct ataataagta
      361 ttatcggatt gtattgatca ggaaaaagtt atgataaaac gaattcctct atatgtcaaa
      421 atattagcag gtctcgccat tggaattttt gtcggtatta ttgctgtaat taccaatagc
      481 ggtgggttta ttatcgatta tgtaaaacca tttggaacga tctttttaaa tcttttgaaa
      541 ttgattgcca ttccattgat ttttacttca ttggtttcag gtatagccgg attatccaat
      601 attaagaagt tttctacatt aggagtacaa accttcgttc tatatattgt aactactgtt
      661 attgcagtat gtatcgggtt gggattggtg aatttgatcc gtcctggtga ttatttttcg
      721 gaaggcaaac gttctgaaat gatggcaaaa tatggccagt ctatttccat cgatgagaag
      781 aatgttcaaa atctcggaca cagatcccct ttacaatttt tagtggatat ggtacctgaa
      841 aacatcgtgg atgcagcttc aagcaatact tccatgttgc aaatcatcgt atttgccgtg
      901 ctttttggat tggccttgat cagcattaat gagcaatttt ccgaacctgt aaagaagttt
      961 attgttgggc tcaatgaagt catacttaaa attgtcgatt ttatcatgct gatagcccc
//
LOCUS       DAORPD010000802         1017 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_32787_length_1017_cov_2.822245, whole genome shotgun sequence.
ACCESSION   DAORPD010000802 DAORPD010000000
VERSION     DAORPD010000802.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32787_length_1017_cov_2.822245"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..>1017)
                     /gene="ychF"
                     /locus_tag="PK976_08665"
     CDS             complement(60..>1017)
                     /gene="ychF"
                     /locus_tag="PK976_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HRC68002.1"
                     /translation="DDRLQKIDALIKSARIVPTTVDIVDIPGLVRGAGKGEGVGSKFL
                     ADIRNTDALIHVLRCFDEPSVPHVESSIDPVRDIEMVELELIVRDLESIEKKLQRLEK
                     QAKSGDREARTGIEVLEVYKHHLEDFLPARTAPVGKEFQRYLTDLFLLSAKPVMYVCN
                     VDDDSAITGNAYSNAVRDFFDDQNVEILTIAGKLESEIAELDDPADRRAFLEEVGLNE
                     PGVNKLIQSAYRLLNLQTFFTAGPKEVRAWTIRKGITAPEAAGVIHSDLERGFIRAEV
                     MKYDDFIRLGSEHACREAGKLFIEGKNYVVQDGDILHIRFNV"
BASE COUNT          265 a          256 c          200 g          296 t
ORIGIN      
        1 ggaagattct gcgaataatt ttcgattcct atgaaggcag tttacgcctc gatgccacct
       61 tatacattaa accggatatg caggatatcg ccatcttgaa ccacataatt ctttccttcg
      121 atgaatagtt taccggcctc acggcatgcg tgttcagagc ccaatcgaat aaaatcgtcg
      181 tatttcatca cttcagctct tatgaatccg cgttcgaggt cggaatgaat gacgccagca
      241 gcttctggtg ctgtaattcc tttgcgaatg gtccatgctc ttacttcctt gggtcctgcc
      301 gtgaaaaaag tctggagatt cagcaggcga tatgccgatt gtatcaattt attaacgcca
      361 ggttcattca agcctacctc ctctaaaaat gcccgacgat cggccgggtc atccaattcg
      421 gcaatttctg attccaattt tcctgcaata gtcaatattt caacattctg atcgtcgaag
      481 aaatctctta cggcattact gtaagcattt cccgtaatag cagaatcgtc atcgacatta
      541 cacacataca tgacgggttt tgcactcaag agaaaaaggt cggttaaata acgttggaat
      601 tcttttccta caggagctgt gcgtgcagga agaaaatcct ccaaatgatg tttatatact
      661 tcgagcactt cgataccagt tcgagcctcc ctgtcgccgc tctttgcctg tttctcgagc
      721 cgctgtagtt ttttctcgat cgattcgagg tcacgaacga taagttcgag ttctaccatc
      781 tcgatgtctc ttacagggtc gatgctgctt tccacatgag gcaccgaagg ttcatcgaaa
      841 caacgcaaaa catgaatcaa agcatcggta tttcgtatat cggccagaaa tttactccct
      901 acaccttcgc ctttaccagc tcctctcacc aaacccggta tatctacgat atctaccgtg
      961 gtaggaacaa tcctggccga ttttatcaaa gcgtcgatct tttgtaaacg atcatcg
//
LOCUS       DAORPD010000803         1017 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_32789_length_1017_cov_2.692308, whole genome shotgun sequence.
ACCESSION   DAORPD010000803 DAORPD010000000
VERSION     DAORPD010000803.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32789_length_1017_cov_2.692308"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="PK976_08670"
     CDS             <1..197
                     /locus_tag="PK976_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC68003.1"
                     /translation="YLMRWFLPMIMRWWTRHFFNQMGMQDAPSKKKKNKVEIKSAPRK
                     IPNKSIMDEIGEYTDYEEIE"
     gene            225..458
                     /locus_tag="PK976_08675"
     CDS             225..458
                     /locus_tag="PK976_08675"
                     /inference="COORDINATES: protein motif:HMM:NF036808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC68004.1"
                     /translation="MAYSSIILKKGKQEPLLRKHPWIFSGAIHHHEGEVNVGDIVAVY
                     SFDRQLMGYGLFEEGSLAVKMISFGTSPDEEDF"
     gene            498..788
                     /locus_tag="PK976_08680"
     CDS             498..788
                     /locus_tag="PK976_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC68005.1"
                     /translation="MGLIDNPTTNAYRFINSEGDGMPGLVADYYDSMILFQAQSSGMQ
                     MLIPTLAESLRSIFQNHLGIIYSKAAEYTNHFFQEQPPQRRIFMGECRKCSD"
BASE COUNT          332 a          173 c          222 g          290 t
ORIGIN      
        1 ggtatctcat gcgttggttc ctcccaatga taatgcgctg gtggacaagg cattttttta
       61 accaaatggg catgcaagat gctccttcaa aaaagaagaa aaataaagtg gaaataaagt
      121 cagctccccg aaagatccct aataaatcta tcatggatga aataggagaa tatacagatt
      181 atgaagagat agagtaaaag ctatccaata agaaaactgg ggaaatggcc tattcgtcga
      241 tcattttgaa aaaaggaaaa caagagcctt tattgcgaaa acatccatgg attttttcgg
      301 gtgccattca ccatcacgag ggtgaagtaa atgtgggtga tattgttgct gtttattcat
      361 tcgaccggca gcttatggga tatggactat tcgaagaggg ttcgcttgcc gtaaaaatga
      421 tctcttttgg aacatcgccc gatgaagaag atttctagca aaaaaaattc gaaacagcct
      481 tctctttaag aaaagctttg ggtttgatcg ataatccaac taccaatgct taccgtttta
      541 ttaattctga aggcgatgga atgccagggt tagtagccga ctattacgat agtatgatat
      601 tatttcaagc tcaatcatca ggcatgcaga tgcttatccc tactcttgcc gaatcacttc
      661 gatcgatttt ccaaaatcat ctgggaataa tttattcaaa agcagccgaa tatacgaacc
      721 attttttcca agaacagcca cctcaacgaa ggatttttat gggggaatgc agaaaatgta
      781 gtgattaaag aacatggatt ttaattcgaa gttgatttta ttaatggtca aaagacaggc
      841 ttttttctcg atcaaaagga aaataggcaa aaagtgagca gatatgccga tggacgttca
      901 gtgttgaatt gttttagtta caccggtgca ttcagtgttt acgcttttgg aggtggtgca
      961 cttagtgtag attctgtgga tagcagtggc aatgccatcg aaaaatgcga acgaaat
//
LOCUS       DAORPD010000804         1017 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_32804_length_1017_cov_2.129938, whole genome shotgun sequence.
ACCESSION   DAORPD010000804 DAORPD010000000
VERSION     DAORPD010000804.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32804_length_1017_cov_2.129938"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1017
                     /locus_tag="PK976_08685"
     CDS             <1..>1017
                     /locus_tag="PK976_08685"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HRC68006.1"
                     /translation="TIEKQIKKIEGVQEVSANLALENVSISYDPDKTSPAEFVEKILK
                     AGYQTPLSSSQSANSSSVTFEEMNVSKLRQVWIAALPILIFGIFFNNFRPGQFLSFFI
                     TVYLMVFPARYIYKKAWRQLKSLTPAMESLVAISTLAALIFSAVILWIPAMHGGHLWF
                     ETPAMIIAFVMTGKFLEERAKRQSAASLQKLKEIQPNKARVIRNGQEIEIEVASAMTN
                     DRVLVKAYEMIPVDGKILIGSSHIDESSITGESMPRFKTKGDEVYAGTRCLEGALTLL
                     VTRSPSETVLANVIAAVTRALSTKAPAQRLADRIAGVFVPVALLISLISFAAWMFFQK
                     EGQF"
BASE COUNT          301 a          220 c          220 g          276 t
ORIGIN      
        1 ccaccatcga aaaacaaatc aagaaaattg agggagtaca agaagtgagc gccaatctgg
       61 cactcgaaaa tgtgagcatt agctacgacc ccgataaaac cagccctgcc gagtttgttg
      121 aaaaaatcct gaaggctgga tatcaaactc ctctctcttc gagccagagt gcaaattcat
      181 cctcagtaac tttcgaagaa atgaatgtga gtaaattacg ccaggtatgg attgcagccc
      241 tacctatcct aatattcgga atatttttca ataattttcg tcctggacaa tttctctcct
      301 tctttattac cgtttatctg atggttttcc ctgcccgata tatttacaaa aaggcttgga
      361 gacaactgaa atctcttact ccggctatgg aatctctggt agccataagc actttagccg
      421 cactcatctt ctcagcagtt attctatgga ttcctgcgat gcatggtggc catctttggt
      481 tcgaaactcc agctatgatt atagctttcg taatgacagg aaaatttctc gaagaaaggg
      541 ctaaacgaca aagtgcagct tcacttcaga aactcaaaga aattcaaccc aataaggcga
      601 gagtaattcg aaacggtcag gaaatagaaa tagaggtagc cagtgcgatg acaaatgata
      661 gagttttagt gaaggcctac gaaatgattc ctgtcgacgg gaaaattctc ataggaagct
      721 cacacataga tgaaagttcg attaccggcg agtcaatgcc aagattcaag accaaaggag
      781 atgaggttta tgccggaaca agatgcctgg aaggagcatt gacgttgttg gttactcgta
      841 gcccttctga gactgtttta gcgaatgtta ttgcagccgt aacacgtgcg ctctcgacaa
      901 aagcacccgc tcaacgcctt gccgaccgta ttgcaggagt tttcgtgccc gttgcccttt
      961 tgatttcttt gataagtttt gctgcttgga tgttttttca aaaagaggga caattcg
//
LOCUS       DAORPD010000805         1017 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_32812_length_1017_cov_1.831601, whole genome shotgun sequence.
ACCESSION   DAORPD010000805 DAORPD010000000
VERSION     DAORPD010000805.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32812_length_1017_cov_1.831601"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="PK976_08690"
     CDS             complement(<1..407)
                     /locus_tag="PK976_08690"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRC68007.1"
                     /translation="MKFPIKILYAEDERLFREMMTDFLKDLAEEVHVADNGNSGMQLF
                     HKHGADLVVADIAMPGLSGLKMIRSIKREKPSIRSIITTSYEETSYFIDAIEIGVDKF
                     LLKPFDRDLLNKTIHELANIILNEQKLLKEEEAR"
     gene            538..>1017
                     /gene="trmD"
                     /locus_tag="PK976_08695"
     CDS             538..>1017
                     /gene="trmD"
                     /locus_tag="PK976_08695"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005787862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HRC68008.1"
                     /translation="MRIDILTLFPGMFEGPFSESILKRATEKKLVEIHVHNIRDYSTD
                     KHRRVDDYPFGGDAGMVMMIEPIYKAIKTLKKERAYDRVIFTTPDGELFDQKMANYFS
                     TLQNIIILCGHYKGIDQRIRDHLIDQEVSIGNYVLTGGELAAAVMTDAIVRLIPGVLH
                     "
BASE COUNT          306 a          196 c          196 g          319 t
ORIGIN      
        1 cgggcttctt cttccttcag cagcttctgc tcattcaata tgatgtttgc taattcatga
       61 atggttttgt tcaacaaatc cctatcgaaa ggtttcaata aaaacttatc cactcctatt
      121 tcaatggcgt cgataaaata actcgtttct tcgtaggagg tggtaatgat agatcttatg
      181 gaaggttttt ctctttttat gcttcgaatc atctttaagc cgcttaggcc aggcatggca
      241 atatcggcta ctactaagtc ggctccgtgt ttatgaaata gttgcatacc cgaatttcca
      301 ttatcggcca catgaacttc ttcggccaaa tccttcagaa aatcggtcat catctcacga
      361 aacaaacgct catcttctgc atacaatatt tttataggga acttcacgag ctaaatttca
      421 attatttcgt catatatgag tgggtttgat aaatttgcag gttctactaa aagagcaaaa
      481 tttttacaaa aataaaactt tcattccaat tatggttatt tttcgaaaaa tttttttatg
      541 cgaatagata ttctaacctt atttcccgga atgttcgaag ggcctttctc cgaatccatt
      601 ctcaaaagag caaccgaaaa aaagttggta gaaattcatg tacacaacat acgagactac
      661 tcgactgata aacaccggcg tgtggatgat tatccctttg ggggtgatgc gggtatggta
      721 atgatgatcg aaccaattta caaagcaata aaaactttga agaaggaaag agcatacgac
      781 agggtcattt ttactacacc cgatggtgaa cttttcgatc aaaagatggc caattatttt
      841 tcgaccttgc agaacatcat cattttatgt gggcattaca agggaatcga tcaaaggata
      901 cgagatcatt tgatcgacca ggaagtttcg ataggaaatt atgtattgac tggtggagag
      961 cttgctgctg cagtaatgac cgatgccatc gtcagactga ttccgggagt tttgcac
//
LOCUS       DAORPD010000806         1017 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_32828_length_1017_cov_1.480249, whole genome shotgun sequence.
ACCESSION   DAORPD010000806 DAORPD010000000
VERSION     DAORPD010000806.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32828_length_1017_cov_1.480249"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(186..971)
                     /locus_tag="PK976_08700"
     CDS             complement(186..971)
                     /locus_tag="PK976_08700"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC68009.1"
                     /translation="MKLLEKPILIVQLIFFLFSSVIALSQGINQIDAQGRKQGVWRKS
                     YENGQIRYEGNFQNDQPVGEFRHYHPNGKLKAIIIHTGDGHSSAAEFYNENEILMARG
                     FFYDEWKDKTWEYFNQKNGKLISIENYQQGIPHGEWIVYYKEDTVVSENFTYDHGQKE
                     GPWKQFFADGKLKLDASYLHHQLDGPYSLYSPEGKEVVKGFYQNGKKMGEWEFYTNDG
                     QLKRIEWYDQNEKIKEEIFIPEEPIKDVPLDPLLDPENQIRIP"
BASE COUNT          267 a          203 c          186 g          361 t
ORIGIN      
        1 caaatttacg aatttcctcc aatttagtat tctcttttat gaaaaattat tttggagatt
       61 ttcgactgct atgtgatcgg agatgttatc atacttttta ttacgtcttg gatgaagtat
      121 agtaaggttc agaatcgatc atccattttc gaggaagcaa aaaattaatc tggcgaattg
      181 aatatctatg gtatcctaat ttgattttca gggtcgagca agggatctaa tggaacatcc
      241 ttgatgggct cttcgggtat gaatatttct tcttttatct tttcgttctg atcataccac
      301 tcgatgcgtt tcagttgccc atcgttggta tagaattccc attctcccat cttttttcca
      361 ttctggtaaa atccttttac aacttccttg ccttccggag aatataggga gtatgggcca
      421 tctaactgat gatgcaaata gctagcatcg agtttcaatt tcccgtcagc gaaaaattgt
      481 ttccatgggc cttctttctg tccatggtcg taagtgaagt tttcacttac gacagtgtct
      541 tccttatagt aaacaatcca ctctccgtga ggaataccct gttgataatt ttcgatggaa
      601 atgagtttcc cgtttttctg gttaaaatat tcccatgttt tgtctttcca ttcatcgtag
      661 aagaaacctc tggccataag aatttcattt tcgttataaa actcggccgc cgaagaatgt
      721 ccatcgcctg tatgaataat aatggctttc aactttccat tggggtgata atgccggaat
      781 tcacccacgg gctgatcatt ctgaaagttt ccctcataac gaatctgtcc attttcgtac
      841 gactttctcc ataccccctg ttttcggcct tgagcatcta tctggttgat tccttgcgat
      901 aaagctatta cagaagaaaa aagaaaaaaa attagctgga caataagaat aggtttctcg
      961 agtagtttca tttcgaattc atttatacgt tctgcgatgg cattttatag tcttggt
//
LOCUS       DAORPD010000807         1016 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_32854_length_1016_cov_2.287201, whole genome shotgun sequence.
ACCESSION   DAORPD010000807 DAORPD010000000
VERSION     DAORPD010000807.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32854_length_1016_cov_2.287201"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="PK976_08705"
     CDS             <1..213
                     /locus_tag="PK976_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006953298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L17 family ribosomal protein"
                     /protein_id="HRC68010.1"
                     /translation="GYTRILKLGFRKGDGAEIAMMELVDFNELMLTGKTPKTGKTQRT
                     RRGGRKRGTTGTSDEPKTPKKKETIK"
     gene            294..>1016
                     /gene="eno"
                     /locus_tag="PK976_08710"
     CDS             294..>1016
                     /gene="eno"
                     /locus_tag="PK976_08710"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001079.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HRC68011.1"
                     /translation="MSNIAFISARQILDSRGNPTVEVDVITDDGVLSRAAVPSGASTG
                     IHEAVELRDEDPSYYLGKSVLKAVENVNTVLRDELKGMYIFDQNEIDARMIEIDGTPN
                     KGKIGANAILGVSLAVAKAAAIEANLPLFHYLGGPNANTLPIPMMNILNGGSHADNSI
                     DFQEFMIMPVGAMSFNDALRMGSEVFHHLAKVLKKAGYSTNVGDEGGFAPNLKSNEEA
                     IQLILQAIEQAGYKPGEDICIAL"
BASE COUNT          309 a          212 c          235 g          260 t
ORIGIN      
        1 ggttataccc gtattttgaa attaggtttc agaaagggcg atggagcaga aattgccatg
       61 atggaactgg ttgatttcaa cgaattgatg ttgacaggaa aaactccaaa aaccggcaaa
      121 actcagcgta cacgccgagg aggccgcaaa agaggtacta ccggcacatc tgacgagcca
      181 aaaactccga aaaaaaagga aaccattaag taaaaccgat cgaaattaaa tgccagatat
      241 ctccatcttt gtgaattttt tagggtattc aaacataaaa taaattaaaa acaatgagta
      301 atattgcctt tatcagtgct cgtcaaatcc tcgattcgag agggaatcca actgttgagg
      361 tagatgtgat taccgatgat ggagttttaa gccgagctgc cgtcccttca ggagcatcta
      421 ctggaattca tgaggctgtc gaactgcgcg atgaggatcc ttcttattat cttggcaaaa
      481 gtgttctgaa ggcggtcgaa aatgtgaata ctgttttacg cgatgaattg aaaggaatgt
      541 acattttcga tcagaatgaa atcgatgccc gaatgatcga aatagatgga acgcccaaca
      601 aaggaaagat aggagcaaat gccattctgg gtgtttcact ggctgtggcg aaggctgccg
      661 ccatcgaggc caatctcccc cttttccatt atctcggtgg cccgaatgca aataccctcc
      721 ctatccccat gatgaatatt ctaaatggag gctctcatgc cgataattcc atcgattttc
      781 aggaattcat gatcatgccg gtgggggcga tgagttttaa tgatgcactc cgcatgggct
      841 ccgaagtctt tcatcatctg gccaaagttc tgaagaaggc aggttattct accaatgtag
      901 gcgatgaggg cggtttcgca cctaatttga aatcaaacga agaagctatt cagcttatcc
      961 ttcaggctat cgaacaagct ggctataaac ccggtgaaga tatttgcata gccctc
//
LOCUS       DAORPD010000808         1012 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_33116_length_1012_cov_2.339603, whole genome shotgun sequence.
ACCESSION   DAORPD010000808 DAORPD010000000
VERSION     DAORPD010000808.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33116_length_1012_cov_2.339603"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..989)
                     /locus_tag="PK976_08715"
     CDS             complement(<1..989)
                     /locus_tag="PK976_08715"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 92 protein"
                     /protein_id="HRC68012.1"
                     /translation="MKGGTKNQLTIFYTALYHAMLQPNIFMDVDRRYRGIDRKVDTAD
                     DFDNYTVFSLWDTYRAWHPLMTIIDSGRTVNYIRTMLNHFDKSGALPVWELAGNETWC
                     MIGNHSIPVIADAWVKGIRGFDPDRALEAMIRSVNSDRFGMDIYRRMGCLLGDKEHES
                     VSKTLEYAYDDWCIAQIAKDLGNDTVYSEFIKRAQFYKNLYDPSTGFIRPKINGGWLT
                     PFDATKVDWNYTEANAWQYNFHIPQDVNGHIALMGSDVAYLTKLDELFNTSQKVSGRD
                     MKDISGLIGQYAHGNEPSHHVAYLFSYAGQPWKTQEKVRQIMDDFYTNAPDGL"
BASE COUNT          276 a          222 c          212 g          302 t
ORIGIN      
        1 atcaaaccat cgggagcgtt ggtatagaaa tcatccataa tttgtctgac tttttcttga
       61 gttttccaag gttgaccggc ataggaaaag agataggcca cgtgatggct gggttcgtta
      121 ccgtgggcat attgtccgat taatccactg atatctttca tatcgcggcc tgaaactttc
      181 tggcttgtgt tgaaaagctc gtccagtttg gttagatatg ccacgtcact tcccataagg
      241 gcaatatggc cattcacatc ttgaggaatg tgaaaattat attgccaggc attggcttcg
      301 gtataattcc aatccacttt cgtagcgtcg aagggagtaa gccaaccacc gtttatttta
      361 ggtcggatga aacctgtaga aggatcatag agattcttat agaactgtgc tcttttaata
      421 aattcagaat agaccgtatc atttcctaag tcttttgcta tttgagcaat acaccagtca
      481 tcgtaagcat attcaagcgt tttggagaca gattcatgtt ctttatctcc tagcaggcat
      541 cccatgcgac gatagatatc cataccaaaa cgatcggaat taacgcttcg aatcatggct
      601 tccaaggcac gatcagggtc gaagcctcta atgcctttta cccatgcatc agcaattacc
      661 ggaatggaat gattgccaat catgcaccat gtttcgttgc ccgcaagttc ccaaaccggc
      721 aaggcaccgc ttttatcgaa atgatttagc atagttcgaa tgtaattgac cgtgcggccc
      781 gaatctatta tcgtcatgag tggatgccat gccctgtatg tatcccaaag tgaaaatacc
      841 gtataattat cgaaatcgtc agccgtatca acctttcgat ctattccacg ataacgacga
      901 tcgacatcca tgaagatgtt gggctgaagc atagcatggt aaagtgcagt ataaaaaatc
      961 gtcagttggt ttttcgttcc ccctttcatc ttgattttgg cgagttcctt at
//
LOCUS       DAORPD010000809         1012 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_33119_length_1012_cov_2.179728, whole genome shotgun sequence.
ACCESSION   DAORPD010000809 DAORPD010000000
VERSION     DAORPD010000809.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33119_length_1012_cov_2.179728"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1012)
                     /locus_tag="PK976_08720"
     CDS             complement(<1..>1012)
                     /locus_tag="PK976_08720"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HRC68013.1"
                     /translation="NKPLLLYAIPVCMVPLIARIFFDTRVALYVHIITIILIGFLVPN
                     SFEFIFLQLISGLITIFSVANFHRRQQFVITAIYVFLAYSAVYFGLYLIQEGHFSGLK
                     SFNFALFGGASALLLLSYPVVYVLEKAFGMLTDITLLEISDVNYKPLKELSSRAPGTF
                     HHSIQVANLAEEVIHQIGGNPLLVRAGALYHDIGKMDMPMYFIENQYTGFNPHEELSP
                     EESAEIILNHVRNGIEKARKYRLPEQIIDFIRTHHGTRRVEYFYRISQQMELESEVNE
                     QAFTYPGPVPFSKETCVLMMADSVEAASRSLHQTDETHIETLVEQLIDSMMKAHQFDN
                     SNI"
BASE COUNT          312 a          204 c          214 g          282 t
ORIGIN      
        1 gatgttggaa ttatcgaatt gatgcgcttt catcatcgaa tcgatcaatt gttcaacgag
       61 ggtttctatg tgggtttcat cggtttggtg taaacttctc gaagcggctt cgacgctgtc
      121 ggccatcatc aatacgcagg tttctttcga aaaaggcacg ggacccgggt aagtaaatgc
      181 ctgttcattt acttccgatt ctaattccat ttgctggctt attctataga aatattcaac
      241 tcttcgtgtt ccgtgatggg ttcggataaa atcaatgatc tgctcaggca gcctgtattt
      301 gcgtgctttt tctattccat ttcgaacatg attcaaaata atttcagcac tctcttcggg
      361 tgaaagttct tcgtgaggat taaaaccagt gtattgattt tcgataaaat acatcggcat
      421 atccattttc ccaatatcgt gatacaaagc ccctgccctc accagaagag gattcccacc
      481 gatttgatga atcacctcct cggccagatt ggctacctgt atggaatggt ggaatgttcc
      541 gggtgcacgt gaggaaagct cttttaaagg tttataattc acatcgctga tctcgagcaa
      601 ggtaatatcg gtgagcattc caaaagcctt ttcgagaaca tataccactg ggtaagaaag
      661 aagtaataaa gcagaagctc cgccaaaaag agcaaagttg aatgacttca agcctgaaaa
      721 atgcccttcc tgaatgagat acagaccaaa atatactgca gagtatgcca gaaatacata
      781 aatggcagta attacaaatt gctgccgcct gtgaaagttg gccacactaa atatggtaat
      841 caatcctgag ataagctgca gaaagataaa ttcaaaacta ttgggcacaa gaaatccgat
      901 caggataatg gtaataatat gtacataaag agctacacgc gtatcgaaaa atattcttgc
      961 aatcaatggc accatacaaa ccggaatggc atagagcaga aggggtttgt tg
//
LOCUS       DAORPD010000810         1011 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_33201_length_1011_cov_1.837866, whole genome shotgun sequence.
ACCESSION   DAORPD010000810 DAORPD010000000
VERSION     DAORPD010000810.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33201_length_1011_cov_1.837866"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /gene="guaA"
                     /locus_tag="PK976_08725"
     CDS             complement(<1..574)
                     /gene="guaA"
                     /locus_tag="PK976_08725"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HRC68014.1"
                     /translation="MHETIVILDFGSQYTQLIARRIRELNVYCEIFPFHIIPKLNPSI
                     KGVILSGSPYSVNQSGAPSPDLSGIRGKVPILGICYSAQYISKISGGCVEVCDTREYG
                     RANLHYINPNNPLMRGLSPNTQVWMSHGDTILEVPDNFEIIASTADVKVAGFEIRNEK
                     IWGIQFHPEVIHTVEGKTLLKNFVVDICHCS"
     gene            677..>1011
                     /locus_tag="PK976_08730"
     CDS             677..>1011
                     /locus_tag="PK976_08730"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HRC68015.1"
                     /translation="MNESDKILLPPSHMIYPLPAVLVSCGTLQHPNAITIAWTGTICS
                     KPPMCYISVRPERYSHHLIKETGDFVINLCDESMLEMLDWCGIHSGRKVDKFKEMNLS
                     ALRAVKVEAP"
BASE COUNT          309 a          203 c          193 g          306 t
ORIGIN      
        1 gggagcaatg acaaatatca actacaaaat ttttcagcaa agttttgcct tcaaccgtat
       61 gaataacttc tggatgaaac tgtatgcccc aaattttttc attccttatt tcaaaaccag
      121 ctacctttac atcggctgtc gatgcaatga tttcgaaatt gtcagggact tcgagtattg
      181 tgtctccatg cgacatccag acctgagtat ttgggcttag gccacgcatt aaaggattat
      241 tcgggttgat ataatgaaga tttgccctcc catattcacg agtatcgcat acttcgacgc
      301 agcctcccga gatttttgag atgtattgag cactataaca tattcccaga ataggaacct
      361 tgcctctgat ccccgataaa tcgggtgagg gtgcaccaga ttgattcacc gaatatggac
      421 taccactgag aattacgcct tttatagaag ggttcagctt tggaattata tggaaaggaa
      481 aaatttcaca atatacattc aattcacgta ttcgcctcgc aatcaattga gtatattgtg
      541 aaccaaaatc cagaatgact atggtttcgt gcatgtaaaa taattaattg agaaacccga
      601 aatttgcgca aaattacata tcttgtggtt gaaaaaccaa ttcattttat attgtactat
      661 ctaaaacttc gatgcaatga atgaatctga taaaatctta cttccacctt cgcacatgat
      721 ctatcctctt cctgcagttt tggtttcatg tggtacattg caacatccaa atgcgattac
      781 gattgcatgg acaggaacga tttgctctaa accaccaatg tgttatatct ctgtgcgacc
      841 cgaaagatat tctcaccatt taattaagga aacaggggat tttgtgatca acctttgcga
      901 tgaatccatg ctggaaatgc tggattggtg tggcattcat tcaggacgaa aagtagataa
      961 gttcaaggaa atgaatctaa gtgctttacg agccgtcaag gttgaagctc c
//
LOCUS       DAORPD010000811         1009 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_33293_length_1009_cov_2.545073, whole genome shotgun sequence.
ACCESSION   DAORPD010000811 DAORPD010000000
VERSION     DAORPD010000811.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33293_length_1009_cov_2.545073"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="PK976_08735"
     CDS             <1..529
                     /locus_tag="PK976_08735"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HRC68016.1"
                     /translation="GMPGPESLLRCHYGTQIAKRYPEAKIIIALPTISEDIYKSHAHK
                     MLEEMVIHGIDSSRFLFETEGTNTREQAKNIAKLKGVGAKDCFLIITSPEHVFRSVKV
                     FRKVGLENCGGLPSFSKELPTELLLSEDENLQKQLPPDRSPSLRYNMWNYLKLEISVL
                     REYVAIVWYWLNGWI"
     gene            complement(533..>1009)
                     /locus_tag="PK976_08740"
     CDS             complement(533..>1009)
                     /locus_tag="PK976_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC68017.1"
                     /translation="IIGPGDWQKGSSQLFTEIYHGLKYYTEGVTGYVDVRDVADTLIL
                     LMKSEIINERFILNSENLCFKDLFDMMADALGKPRPMVRINKFTAEIAWRLFTLKSLV
                     TGKPSAITAETARSAFVSYYYSNQKFKETFPNYSFIPIRNSIADTAKIFITEHSLS"
BASE COUNT          316 a          184 c          209 g          300 t
ORIGIN      
        1 tggaatgcct gggcccgaga gtcttttgcg atgtcattat ggaacgcaaa tcgcaaaaag
       61 ataccctgag gcaaaaatca tcatcgcact ccccaccata tctgaagata tttataagag
      121 ccatgctcac aaaatgcttg aagaaatggt tattcatggc atcgatagca gccgtttcct
      181 atttgaaaca gaaggaacca ataccagaga acaagcaaag aacatagcaa aacttaaggg
      241 tgtgggtgcc aaagattgct ttcttataat tacttctcct gaacacgtat ttcgtagcgt
      301 taaagtcttt cgcaaggtgg gactcgaaaa ttgtggaggg cttccttcat tttctaaaga
      361 actacctaca gaacttttgc tttcagaaga tgaaaatttg cagaaacagc tcccaccaga
      421 tcgttctcct tctcttcgat ataatatgtg gaactatttg aagttagaaa tatctgtttt
      481 gagagaatat gtagctattg tttggtattg gctgaatggg tggatataag attcaagaca
      541 aagaatgttc agtgataaag attttggctg tatcggctat agaatttcta atgggtatga
      601 atgaatagtt tgggaaggtc tctttgaatt tttgattgga ataataataa gacacgaagg
      661 ccgagcgagc ggtttctgcg gtgatagccg aaggtttgcc agtaacaaga ctcttgagtg
      721 tgaataaacg ccaggcaatc tcagctgtaa atttattgat gcgcaccata ggacgaggct
      781 tgcctaatgc atcagccatc atatcgaata aatctttaaa gcataaattt tctgagttga
      841 ggataaaacg ttcgttaatt atttcacttt tcatcaataa aatgagtgta tcggccacat
      901 ctcgaacatc tacatatcct gtaactcctt cagtataata ctttaagcca tgatagattt
      961 cggtgaataa ttgcgaacta cctttttgcc agtcgcctgg tccaataat
//
LOCUS       DAORPD010000812         1008 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_33360_length_1008_cov_2.614900, whole genome shotgun sequence.
ACCESSION   DAORPD010000812 DAORPD010000000
VERSION     DAORPD010000812.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33360_length_1008_cov_2.614900"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..>1008
                     /locus_tag="PK976_08745"
     CDS             71..>1008
                     /locus_tag="PK976_08745"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HRC68018.1"
                     /translation="MKPDELKSVYFLGIGGIGMSSIARYFHQKGISVSGYDRCPSRLT
                     EELMAEGIEIHFEERPACIPSEIELVIYTPAIPADNLEFRYAAEKGIPMMKRSQVLGL
                     LTCEKPTLAVAGTHGKTTISTMAAHILFNSHLGCTAFLGGISKNHQSNFITKNGSNWV
                     VIEADEYDHSFLELKPQIAIITAIDPDHLDIYHDYQNLVNAFKDFAGLISANGLLILK
                     KGLENLLSGSYLCRKATYSFNDTSADYFARDILLEKMKYSYSIQTPEGIIGPVQLKVP
                     AYVNIENSIAAFAAALAAGCSAEEIIRALSDFEGIRR"
BASE COUNT          306 a          227 c          208 g          267 t
ORIGIN      
        1 tcccagatgc tgacgaacga attgcccgag aaattattca aatggtcgaa aattcaaaac
       61 ctgcaaagcc atgaaacctg acgaactaaa atcggtatat ttcctgggca taggaggtat
      121 tggcatgagc tcaattgccc gatactttca tcaaaaagga ataagtgtat cgggctacga
      181 ccgatgccct tcacgcctca ccgaagaact tatggccgaa ggtatagaaa ttcatttcga
      241 agaacgcccc gcttgcatac cttccgagat cgaattggta atctatactc cggctatccc
      301 tgccgataac ctcgaatttc gttatgccgc cgaaaaaggc attcccatga tgaaacgatc
      361 acaggttctg ggattactta cctgcgaaaa acctacactg gcagttgcag gtacacatgg
      421 gaaaaccact atttctacaa tggcggccca tatccttttt aattcacatc tgggttgtac
      481 ggcgtttctg ggaggcataa gcaaaaatca tcaatcgaat ttcattacaa aaaatggaag
      541 caattgggtg gtaatcgaag ccgacgaata tgaccactcg ttccttgagt tgaaaccaca
      601 aattgccata ataacggcta tcgaccccga tcatctggat atatatcacg attatcagaa
      661 ccttgtgaat gcttttaagg attttgcggg cctgatatct gccaatggat tgcttatcct
      721 gaaaaaagga ctcgaaaatc ttctatctgg cagttattta tgccgcaaag cgacttactc
      781 attcaatgat acttcggccg attattttgc cagagatatt ttgctcgaga aaatgaaata
      841 ttcctactct atacaaacac ccgaaggaat tattggccca gtacaattaa aagtgcccgc
      901 ttatgtaaat atcgaaaaca gtattgcagc ctttgctgca gcccttgcag cgggatgcag
      961 tgcagaagag attattcggg ctttgtcaga tttcgaagga attcgtcg
//
LOCUS       DAORPD010000813         1008 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_33398_length_1008_cov_1.436516, whole genome shotgun sequence.
ACCESSION   DAORPD010000813 DAORPD010000000
VERSION     DAORPD010000813.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33398_length_1008_cov_1.436516"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(179..436)
                     /locus_tag="PK976_08750"
     CDS             complement(179..436)
                     /locus_tag="PK976_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC68019.1"
                     /translation="MKEIRILGILITDRIKEAGQTQKVLSEFAHIIKSRLGFHEVTEA
                     VCSRLGIIMLQLVGSIDEQDKLESALNQIGGIQVRRMQFEV"
     gene            complement(542..>1008)
                     /locus_tag="PK976_08755"
     CDS             complement(542..>1008)
                     /locus_tag="PK976_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541400.1"
                     /note="in Escherichia coli this enzyme functions in
                     thiamine biosynthesis along with thiFSGI and IscS; with
                     ThiFSG catalyzes the formation of thiazole phosphate from
                     tyrosine, cysteine and 1-deoxy-D-xylulose-5-phosphate;
                     forms a complex with ThiG; contains an iron-sulfur center;
                     in Thermotoga this enzyme has an extra C-terminal domain;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HRC68020.1"
                     /translation="ENPRLRREVIPFGISQIDGGTRLELGTYSQNLNAEQDLNREQFK
                     INDDRSLNEIIEELIQNDFIPSFCTACYRIGRTGEHFMEFSVPGFIKRFCTPNALLTL
                     SEYLVDYAPQDTAQMGWKAIEQNLAHLGDTANTQEIRRRIERIKQGERDLYF"
BASE COUNT          289 a          201 c          194 g          324 t
ORIGIN      
        1 caaaaattcc tggatgagga tggctggatt tatgtagaaa tcaacgaaag atttcccgct
       61 atgatacaaa aaatattttc ttcgcataaa tatcgtaata tcgaaacaag aaaagacttc
      121 aatggaaaat acaggatgat gagagcccaa aaacccgaat gagcatcttc tggtagattt
      181 atacctcgaa ttgcatccgc ctgacttgaa tacccccaat ttgattcaaa gcactttcca
      241 gtttatcttg ttcatctatg gaaccaacaa gttggagcat aatgatcccc agcctcgaac
      301 agactgcttc tgtaacttca tggaagccta aacgcgattt gattatatga gcaaattcgg
      361 ataatacttt ctgggtctgc cctgcttcct ttatgcggtc ggttataaga attcccagga
      421 ttctgatttc tttcatctgt caaagatttt caaatttaat acaaaatatt cataatgaaa
      481 cacttatact aatcttggaa ggctcattcg attcctattc tgtatatagt attttaaaat
      541 tttaaaaata caggtctctt tcgccctgtt tgattctttc gattcgtcgg cgtatttcct
      601 gagtattagc tgtatcgccc aaatgagcaa gattttgttc tatagctttc cagcccattt
      661 gtgcagtatc ttgaggagca taatccacta aatattccga aagcgttagc agcgcatttg
      721 gtgtacaaaa tcttttaatg aatccgggaa cgctgaattc catgaaatgc tcgcccgttc
      781 gccctattcg atagcaagcc gtacagaatg aggggatgaa gtcgttttga atgagttctt
      841 cgatgatttc attgagtgaa cggtcgtcgt taattttgaa ttgttcacgg ttaaggtctt
      901 gttctgcatt aagattttga gaataggtac ccaattccaa gcgtgttccg ccatcgatct
      961 gagaaatgcc gaaggggatt acttctctgc gtagccttgg gttttctc
//
LOCUS       DAORPD010000814         1007 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_33429_length_1007_cov_2.227941, whole genome shotgun sequence.
ACCESSION   DAORPD010000814 DAORPD010000000
VERSION     DAORPD010000814.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33429_length_1007_cov_2.227941"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..>1007)
                     /locus_tag="PK976_08760"
     CDS             complement(140..>1007)
                     /locus_tag="PK976_08760"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HRC68021.1"
                     /translation="KGNGIFHNKKFYYVVYDQAVGLAPTSPVFIRGLQVGKVSDVFFH
                     KNMNGSVVVKLNIVNQFPIPNNSVATIINRDLMGAKAIEIILGDSPHRAQPGDTLAGA
                     IDAGLLEGLNRQLTPVMQKAELALNSIDSTMKVLQVSLNHENRENISKSLQSIQFSLR
                     NIKQITATLDTLILSNKSRVSFIIQNIESISANLESNNEQLTAVISNIHTISDTLAQV
                     HIEQTFKKLDYNLELLSEILIKIERGEGSAGLLLSSDSLYDNLSRSTKELELLIRDIR
                     ENPSRYLKFSVF"
BASE COUNT          291 a          194 c          201 g          321 t
ORIGIN      
        1 tatcatttat ttgataaaac ctgaaggtgg cacgtatccc gagaaacagt ttccggccga
       61 tagattgaaa ctgaaaggct ttgaatggca cgaacaacgt cgcccccgaa aacctgatga
      121 tatcttcatc tggtgagttt caaaaaactg aaaatttaag gtaacgactc gggttttcgc
      181 gaatatcgcg aatcaatagc tccaattcct tggtagagcg cgaaagattg tcgtaaaggc
      241 tgtcactgct tagcaataat cctgccgatc cttctcctcg ttcgatcttt attagtattt
      301 cggaaagcaa ctccagatta taatcgagtt ttttgaaggt ttgctcaatg tgtacttgtg
      361 ccagcgtatc gcttatggtg tgaatattcg agatgacggc tgtaagctgt tcgttgttgc
      421 tttcgagatt tgccgaaata gattctatgt tttgaataat aaaagaaacc cttgatttat
      481 tggaaaggat taaagtatcg agtgtagcgg taatctgctt gatgtttcgt aacgaaaatt
      541 gaatgctttg aagacttttc gaaatatttt ccctgttctc atgattcaat gaaacttgta
      601 gaactttcat tgttgaatca attgaattta aggccagttc ggctttttgc atcacgggtg
      661 tcaattgcct gttcaatcct tccaataaac ccgcatctat agcaccagca agcgtatcac
      721 ctggctgagc acgatgtgga gaatctccaa gaatgatttc aatggctttt gcacccatta
      781 gatcacgatt gataattgta gccactgaat tattgggtat gggaaattga tttacgatgt
      841 tgagttttac tactaccgaa ccattcatat ttttatgaaa aaacacgtcg gaaaccttac
      901 ctacctgcaa ccctcgaatg aagaccggtg atgttggtgc aagcccaaca gcctgatcat
      961 aaactacata gtaaaatttt ttattatgaa atattccatt tcctttt
//
LOCUS       DAORPD010000815         1006 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_33479_length_1006_cov_2.847529, whole genome shotgun sequence.
ACCESSION   DAORPD010000815 DAORPD010000000
VERSION     DAORPD010000815.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33479_length_1006_cov_2.847529"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="PK976_08765"
     CDS             complement(<1..572)
                     /locus_tag="PK976_08765"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRC68022.1"
                     /translation="MRKSFFVIQAFISFLFIGIITLQAQQTQGKKKAVCIAFYNTENL
                     FDTIDDPLTDDKEFLPDGANQWNTSKYMEKLNHLAEVISQIGDDYVKGGPTVLGLSEI
                     ENRRVLEDLVKMPELSKQGYAIIHFDSPDNRGVDVALIYKYHVFKVSSARAVPLHIPD
                     MPNFKTRDQLVVSGMIEGEMLHFIVNHWPSR"
BASE COUNT          329 a          197 c          162 g          318 t
ORIGIN      
        1 cgtgagggcc aatgatttac gataaaatgc aacatttctc cttcaatcat tcccgaaaca
       61 actaattgat cacgagtttt gaaattgggc atgtcgggaa tatgcaaggg aacagcccgt
      121 gcagagctaa ctttgaatac atgatatttg taaattaaag ccacatcaac tcccctatta
      181 tctggtgagt cgaaatggat aatggcataa ccctgttttg aaagctctgg cattttgacc
      241 aaatcttcaa gtacacgacg gttttctatc tccgataaac ccaaaacagt aggaccacct
      301 tttacataat catctcctat ttgagaaatt acttcggcaa gatgattcaa tttctccatg
      361 tatttcgatg tattccactg gttggcacca tcgggtaaaa attccttatc gtcagtaagg
      421 ggatcatcaa tagtatcgaa aagattttca gtattataga aggctataca cactgctttt
      481 ttcttccctt gggtttgctg agcttgtaat gtaattatcc caataaatag gaatgagata
      541 aaagcctgaa tcacaaaaaa ggacttacgc atttgttata ataataattt gcgccaaaaa
      601 tatcaataat ttagatgagt agatttattt aatgtctttg caataagttg atttctgcca
      661 atgtggctta tatttcgaaa cccgacgaat atcgtcattt ttcgagctaa attacggata
      721 tattttatca ttataaaaaa ctcaccgcaa aaaccttctc tctttcacca atatttccat
      781 atttttcgtt cctttgtaac cccaaatttc gaacattcga ttgatgggaa agcataatgt
      841 aaactcacta aggatgaaaa gattcatact tacactttgc ttgggatttt gtttactttc
      901 actcaaggca cagcaaatac aactaaaagg cattctcgtt gatgaaacca cacaatctcc
      961 cattgctaat gcaattatta agattgagga caaaaccatt agcacg
//
LOCUS       DAORPD010000816         1006 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_33511_length_1006_cov_1.726604, whole genome shotgun sequence.
ACCESSION   DAORPD010000816 DAORPD010000000
VERSION     DAORPD010000816.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33511_length_1006_cov_1.726604"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1006)
                     /locus_tag="PK976_08770"
     CDS             complement(<1..>1006)
                     /locus_tag="PK976_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC68023.1"
                     /translation="SIFLVKIRYDGITEWVKWASSVYQGAGAARIFPGDLHLQNPTSI
                     QWFFSSRDSILFNNQSFAPAAYLMGLQADGNLISMVPVMQGVDAIEFSSSEMEHLFVA
                     ESWNDTIFLGDTTFVSIASNNAIYCKFDTAFQLIWSKVVGETGLAIPANGTLDLDGSS
                     YCTGMFMNSILIGNDTFQTLNPSIPDLFLCKFDSLGELMWVKRSESNGTSGASVIGLT
                     SVRSGSLYATGYFSGSARFGTSQITAGTSVDAYIAKYDTAGNCRSLLHFGEASGRSIG
                     WSDDQHLIIGGLFTGDIILGADTISSFNAWDALVVSVDTVLTGIELPANRTAKSLVVY
                     A"
BASE COUNT          311 a          256 c          201 g          238 t
ORIGIN      
        1 tagcatacac cacaagtgac ttcgcagttc tattagctgg caattcgatc ccggtcaaaa
       61 cagtgtctac ggataccacc aacgcatccc atgcgttgaa actggaaatg gtatctgccc
      121 caagaataat atctcccgta aacaagcctc cgataatcag atgttgatcg tctgaccacc
      181 caatgctcct acccgaggcc tcaccaaaat gaagaaggct acgacaattt cctgccgtat
      241 catacttggc tatataagca tctaccgagg ttccagcggt aatttgtgaa gtcccaaacc
      301 ttgctgagcc actgaaatat cctgtagcat ataagctgcc ggatcgaaca gacgtcaagc
      361 ctattacgct tgccccagat gtcccgttcg attctgatcg cttaacccac atcaactcgc
      421 caaggctatc aaacttacat aaaaaaaggt cagggatgga agggttgagg gtttgaaaag
      481 tgtcgttccc aataagaata ctattcatga acattccagt gcaataggaa gatccatcca
      541 aatccaaagt accatttgct ggaattgcaa ggcctgtttc acccaccacc ttactccaaa
      601 tcaattgaaa agcagtatcg aacttacaat aaatcgcatt gttcgatgca attgaaacaa
      661 aagtggtatc acctaagaaa atagtatcgt tccaggattc cgctacaaac aaatgctcca
      721 tttcggaaga agaaaattca attgcgtcga ccccttgcat aactggaacc atgcttatca
      781 gattcccgtc tgcctgcagc cccatcaaat atgcggcagg agcgaatgac tgattattaa
      841 ataggatgga atcccgcgaa gaaaagaacc attggattga cgttgggttt tgcaaatgca
      901 gatcgcccgg aaatattcga gcggcgccgg cgccctggta caccgaactg gcccatttca
      961 cccattcggt aatgccatca taccggatct ttacaaggaa aatgga
//
LOCUS       DAORPD010000817         1003 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_33708_length_1003_cov_1.889241, whole genome shotgun sequence.
ACCESSION   DAORPD010000817 DAORPD010000000
VERSION     DAORPD010000817.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33708_length_1003_cov_1.889241"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            320..>1003
                     /locus_tag="PK976_08775"
     CDS             320..>1003
                     /locus_tag="PK976_08775"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC68024.1"
                     /translation="MSEKELPTRHRNLILTLILITSFANPFMGSSINLALPAIGSHFG
                     LHAVMMSWVAMSFLLSSAVLMVPLGKIADIIGRKRVFLSGTILFSLSSIFCAMSPSVG
                     LLLASRVLQGAGGAMMFGTNMAIITDIFPANERGKAIGTNVMAVYLGLSLGPLIAGFL
                     VKNWGWQSIFYLTAIPVAFVAISMPLLVKTEWKSPHSEQFDWKGGILYIIAMSAFMYG
                     FSHLPNFWAI"
BASE COUNT          259 a          185 c          209 g          350 t
ORIGIN      
        1 caaagtaggc agatattcat attctgccat aacaggcatg gcaacgtata ttttcgatga
       61 agtttttgaa ttcattcagt tatgcatcat ttttgcaaat ttacatcggc gcctctttat
      121 atttcgaact ttgatttttc agcctaatca caatagtgat aacaggtatc gatttgcact
      181 atgataatat aaaaatgctc ttctttgtat ttttgttatg aattgataaa taatgcatta
      241 cgaataaaat taaaggtaaa gactataaga taaagtaaac cattggctca aagcttgtaa
      301 ttttatcaaa acctttgtca tgagtgagaa agaacttccg actcggcatc gcaatttgat
      361 tcttacgctt atactgatta cctcgtttgc aaatcctttt atgggatcat ccattaatct
      421 tgctttacct gcaattggtt cccacttcgg gctccatgct gtgatgatga gttgggtagc
      481 catgtcgttt ctattgtcat cggctgtttt aatggttcct ttggggaaaa tagccgatat
      541 cattggacga aagagagttt tccttagtgg gaccatcttg ttctcattga gttctatttt
      601 ttgtgccatg agtccgagtg tggggctttt attggcttct cgtgtgcttc agggtgctgg
      661 gggtgcaatg atgtttggaa cgaatatggc cattattacg gatattttcc ctgccaacga
      721 aagaggcaaa gccattggta ctaacgtaat ggcggtttat ttgggactgt cgcttggtcc
      781 tcttattgcc ggtttcctcg ttaaaaactg gggctggcaa agcatattct atttaactgc
      841 tattccagtg gctttcgtgg ctatttccat gcctcttctc gtaaaaacgg aatggaaaag
      901 cccacattcg gaacaatttg attggaaagg aggaattcta tatatcatcg ccatgtcggc
      961 atttatgtat ggttttagcc atctcccgaa tttctgggct atc
//
LOCUS       DAORPD010000818         1002 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_33749_length_1002_cov_3.610348, whole genome shotgun sequence.
ACCESSION   DAORPD010000818 DAORPD010000000
VERSION     DAORPD010000818.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33749_length_1002_cov_3.610348"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /gene="folB"
                     /locus_tag="PK976_08780"
     CDS             complement(<1..337)
                     /gene="folB"
                     /locus_tag="PK976_08780"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HRC68025.1"
                     /translation="MAWIALEQMEFWAYHGCFTEEQVIGTRFMIDLYIETDTSKAELS
                     DDLNDTINYQSVYKVVKNEMQIPSKLLEHLGRRILTSVFTRFPKISYAKIKISKLNPA
                     LGGKIGSVSV"
     gene            complement(341..>1002)
                     /locus_tag="PK976_08785"
     CDS             complement(341..>1002)
                     /locus_tag="PK976_08785"
                     /inference="COORDINATES: protein motif:HMM:NF039902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HRC68026.1"
                     /translation="MGYRESGYYPEAFINMLALLGWNPGTEQEIFSLDELIDAFSLER
                     VGKSGSRFDPEKAKWFNQQYLRKRSDDELTQEFIPILKNLGVDCSWEYVKESIHLVRE
                     RVSFVKEIWDQGWFFFKPPEKYDEEVIKKRWKPETSQILQKFSDTLLHLDTFDQVHLD
                     ETIQNFLQENAVEMGALMSPLRICLTGGAIGPHLTEIMALIGKEETLHRIETALKKIN
                     H"
BASE COUNT          273 a          219 c          194 g          316 t
ORIGIN      
        1 taacgctcac actgccaatt ttccctccca aggcgggatt cagtttcgat atctttattt
       61 tagcataaga aatcttaggg aaacgtgtga atacacttgt aaggattctt ctgccaagat
      121 gttcgaggag tttcgatgga atttgcattt cgttcttcac tactttgtac acactctgat
      181 aattgattgt gtcgtttaag tcgtcggaaa gttcggcttt tgatgtgtcg gtttctatat
      241 agaggtctat catgaagcga gtgcctataa cctgttcctc ggtgaagcaa ccatggtagg
      301 cccaaaattc catttgttcg agggcaatcc aagccatgac ttaatggttt atctttttca
      361 gtgcggtttc aattctatgt aaggtttctt ccttgccaat caaggccatt atttcggtaa
      421 gatgagggcc aatagcacct ccagtaagac aaatcctcaa aggactcatc aaagcaccca
      481 tctcaacagc gttttcctga aggaaatttt gtatcgtttc atcgagatgg acttgatcga
      541 atgtatcgag atgcaaaaga gtgtcggaaa atttttgaag aatctgtgaa gtttcgggtt
      601 tccatctttt cttgatgact tcttcatcat acttttcagg aggtttaaaa aagaaccatc
      661 cttggtccca gatttctttc acaaaactta ctctttcacg caccagatgt atgctttcct
      721 tcacatattc ccaagaacaa tctacgccaa gattcttcaa aatgggaatg aactcctggg
      781 ttaattcatc atcagaacgt tttctcaaat attgctggtt gaaccatttg gccttttcag
      841 gatcaaaacg tgaacccgat ttccccactc tttccagtga aaaggcatcg atgagttcat
      901 ccagggaaaa gatttcctgt tcggtacctg gattccagcc aagcaaggcc aacatattga
      961 tgaaagcctc tggataatat cccgattcac gatatcccat cg
//
LOCUS       DAORPD010000819         1001 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_5404
            NODE_33811_length_1001_cov_2.601480, whole genome shotgun sequence.
ACCESSION   DAORPD010000819 DAORPD010000000
VERSION     DAORPD010000819.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867621
            Sequence Read Archive: SRR1022352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,737
            Genes (coding)                    :: 1,726
            CDSs (with protein)               :: 1,726
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 3 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33811_length_1001_cov_2.601480"
                     /isolate="AMR_MDS_5404"
                     /isolation_source="Aquaculture farm sediments"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="PK976_08790"
     CDS             <1..771
                     /locus_tag="PK976_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRC68027.1"
                     /translation="ELARALAEFLFNDENNMVRIDMSEYQERHSVSRLIGAPPGYVGY
                     EESGQLTEAVRRKPYSVILLDEIEKAHPDVFNILLQVLDDGRLTDNKGRTANFKNTVI
                     IMTSNIGSHLIQERIPENAKEIDPKILEQTREEVMDLLKKTIRPEFLNRVDEIIMFKP
                     LTFDEVIQIVRLQFEHLAETLAANEIQLKYTEKAVEYIAKAGFDAQFGARPIKRAMQR
                     EILNVLSKKILAGDVDKDLIIVADATAEGIVFRNEPNK"
BASE COUNT          316 a          204 c          216 g          265 t
ORIGIN      
        1 gaattggcta gggctctggc cgaatttctg ttcaacgacg aaaataacat ggtaagaatt
       61 gacatgtcgg agtatcagga acgtcattcg gtttcgcgct tgattggtgc tccaccagga
      121 tatgtgggtt acgaagaaag tggccagctt accgaagccg tgcgtcgcaa accttattcg
      181 gtcattctgc tcgatgaaat cgaaaaggca catcccgatg tgttcaatat actcttacag
      241 gtactcgatg acggaaggct taccgataat aaaggacgta cagccaattt caagaatacc
      301 gtcatcatca tgacctctaa tattggctcg catttgattc aggaacgtat tcccgaaaat
      361 gctaaggaaa tcgatcctaa gattttagaa caaacccgag aagaagtcat ggatttgctc
      421 aaaaagacga ttcggcccga attcctcaac agggtggatg aaatcatcat gtttaaaccc
      481 ttgacgttcg acgaggtaat tcaaattgtt agattacagt tcgagcattt ggccgaaaca
      541 ctggcagcca atgaaataca gttgaaatat accgaaaagg ctgtcgaata tattgcaaag
      601 gctggttttg atgcacagtt tggcgctcga cctattaaaa gagccatgca gcgtgagatt
      661 ctcaacgtgc tgtcgaagaa aattctcgcc ggcgatgtgg ataaagacct cattattgtg
      721 gccgatgcta cagcagaagg aatagtgttc cgtaatgaac cgaacaaata aaaccaataa
      781 aatttaatct cttggttgtg aggatagtat gtgttaggcg tgctatcctc atttttttat
      841 ttcgaattct catccctcga agaggctttc ttgaaaaacg aaaacggggg agggttcccc
      901 cgtttctcca aactttaacc aaaaacaatt tacaaaccaa tgaaaacaaa aatcaatgtt
      961 tctgtttcat tcgatctgca aagtaactaa aaaacattcg a
//
